DAGLA cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	DAGLA
Entrez Gene ID	569398
Full Name	diacylglycerol lipase, alpha
General protein information	Preferred Names diacylglycerol lipase, alpha Names sn1-specific diacylglycerol lipase alpha nsddr
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021477715.1	XP_021333390.1	sn1-specific diacylglycerol lipase alpha isoform X1
XM_009302951.3	XP_009301226.2	sn1-specific diacylglycerol lipase alpha isoform X2
XM_692781.7	XP_697873.5	sn1-specific diacylglycerol lipase alpha isoform X1
XM_692781.8	XP_697873.6	sn1-specific diacylglycerol lipase alpha isoform X1
XM_009302951.2	XP_009301226.1	sn1-specific diacylglycerol lipase alpha isoform X2
XM_009302952.2	XP_009301227.1	sn1-specific diacylglycerol lipase alpha isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	dagla	XP_697873.5
Homo sapiens (human)	DAGLA	NP_006124.1
Canis lupus familiaris (dog)	DAGLA	XP_005631813.1
Mus musculus (house mouse)	Dagla	NP_932782.2
Gallus gallus (chicken)	DAGLA	XP_423696.3
Xenopus tropicalis (tropical clawed frog)	dagla	XP_004913466.1
Rattus norvegicus (Norway rat)	Dagla	NP_001005886.1
Pan troglodytes (chimpanzee)	DAGLA	XP_508479.4
Bos taurus (cattle)	DAGLA	NP_001179512.1

Gene Ontology

The following DAGLA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAGLA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa45733	XM_009302951.2 Latest version!	Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa45734	XM_009302952.2 Latest version!	Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa144786	XM_009302951.3 Latest version!	Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa144785	XM_692781.8 Latest version!	Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa12691	XM_692781.7 Latest version!	Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa12691	XM_021477715.1 Latest version!	Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DAGLA ( XM_009302951.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301226.1 Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa45733
Clone ID Related Accession (Same CDS sequence)	XM_009302951.2
Accession Version	XM_009302951.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sn1-specific diacylglycerol lipase alpha isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302951.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGCA TGGTACTGTT TCGGCGACGC TGGTCAGTCG GCAGTGATGA CCTGGTGTTG 
CCGGCTTTCT TCCTGTTTGT ACTACACTGC ATATGGCTGG TGGTTCTGTC CGTGGTTCTC 
TTTGGGCTGC CGTACAGTTC AGAGCAGTCA TGTTCAGTCA CGCTGGTGGA TCATGGTCGC 
GGTTACCTCG GCATCTTGGT CAGTTGTCTG ATCTGCGAGA GCGCCATCAT GTGGCTGAGC 
ATGAGGGGCA GCATCCTCTA CACACAACCC AGAGAGGCGG TGCAGTATGT GCTCTATATA 
CGCCTAGCCA TCCTGCTCGT GGAGCTGGTG TATGCAGTGG TGGGCATCGC CTGGTTGGTG 
CAGTACTACC AGCCCTGCCC AGACATCACC GCTAAGAACC TCGCCCTCGG CATTGTGGCA 
TGTAACTGGT TGGTCATCTT CAGTGTGTGT TTCACCATGA TGTGCACCTT TGACCCCACT 
GGACGGACCT TTGTCAAGCT GAAAGCAACT CGTCGACGTC AGCGTAACCT TACAACATAC 
ACACTCAGAC ACAGGCTAGA AGAGGGTCAA GCCAGCAGCT GGAGCAGAAG ACTCAAATTC 
TTCATGTGCT GCACCAGAGC AAAAGACACA CAATCTGACG CTTACTCTGA GGTTGCCAGC 
TTGTTCGCCG AGTTTTTCCG GGATCTGGAT ATCGTGCCGA GTGACATCAT TGCTGGTTTG 
GTGCTGCTCC GACAGAGACA ACGGGCTAAA AGATCAGCCA TCTTGGATCA GGCAAACAAT 
GATGTTTTAG CCTTTCTGTC TGGGATGCCT GTGACACGCA ACACTAAATA CTTGGACCTC 
AAGAACTCGA CTGAGATGGC TATGTATAAG GAAGTGTGCT ACTACATGTT GTTTGCCATG 
GCGGCATATG GCTGGCCCGT TTATCTTTTG AGAAAACCAG CATGTGGACT CTGCAGACTT 
GTCAGCACCT GCTCCTGCAA TACTTCAGTT TCAGGCTCTC GCCTCTCTCA ATCCGTCACC 
GTTGAGGAAG ATAACTGTTG CGGCTGTAAC GTTCTTGCCA TCCGACGCCA GTTTCTAGAC 
CGAGACCTCA AAGAAGTCCA GATTGTCTAC ACATCCTGCC ATGACGCGGT TTATGAGACG 
CCCTTCTTTG TAGCAGTGGA TCACGCAAAG AAGAAAGTCG TGATCAGCAT CAGAGGAACG 
TTGTCTCCTA AGGACGCTTT AACAGATCTC ACTGGCGATT CGGAGCGTTT GCCTGTCGAG 
GAGCAGCACG GCACGTGGCT CGGACACAAG GGAATGGTTT ATTCAGCCGA ATACATCAAG 
AAGAAACTGG AGCAAGAGAT GATCCTCTCT CAAGCCTTTG GGAGAGATTT GAGTAAAGGG 
ACGATGCACT ACGGGCTGGT GATAGTGGGT CATTCTCTGG GAGCGGGAAC AGCCGCCATC 
CTGTCCTTCC TCCTTCGGCC GCAGTATCCT TCACTGCAAT GCTATTCCTA CTCTCCTCCC 
GGCGGCCTGC TTAGTGAGGA TGCAATGGAG TACTCGAAAG AGTTTGTCAC CTCAGTGGTG 
TTGGGAAAAG ATCTGGTGCC CAGGATCGGC CTCTCTCAGC TTGAGGGTTT CCGTCGTCAC 
CTGCTTGAAG TCTTACAGAA GAGCGATAAA CCAAAGTGGA GGATCATTGC TGGAGGAACA 
AAGTGCATCC CTAAATCGGA GCTGCCCATG GATGACGAAG CCCCTGTATC TCAGGGCGTA 
ACACCCTCCA GTTCCCGCCT ATGGCTTCAT CCCAGTGATC TGAGCATCGC CCTGTCAGCC 
TCCACACCGC TGTTTCCCCC GGGACGGGTC ATTCATGTGG TGCACAATCA CCCCCCTGAG 
ATGTGTTGTG GTCAGGAAGA GCCCACGTAC TCTGCTCTGT GGGGCGATAA CAAGGCCTTT 
GATGAGGTCA TCATTTCTCC AGCCATGCTG AATGAACACA TGCCCCATGT GGTGATGGAT 
GGCCTCAACA AGGTGCTGGA AAACCCACAT TTAATTTCAG ACCTGATTGC TGCACAGGTT 
GGAAGCCGGA AAGCTAGCAA TGAAGTGCAT AATTCTGTCC CTAACCCATC CTCAGATCCC 
CCTTTTACAC CACTGTGTCC AGATGAATGC TTTATAGTTC CCAAAACTCC CACTGAAACA 
CTTTCTGTTC AACCACGGGG TTCAGACACT CAAAGGAACG GTTTATCATT CAAAAATTCG 
TCCACTGAAA ATACTGTAAC AGAAACTCCC ACTGCGATCC CATCACCCAC AAAGCCTGTT 
ACTGTAGATA CATCTTCCAT TAGGTCAAAT ACAAAACCTG AAGGCGCTTC TGATGACGTT 
CAGCAATCAT CAACATGTCC ATCTCAGTCC AACACTACAT TGAGCTCACC GAAGTTAGAC 
TTTCCAACAT TAGCAATGCC CAAAACGGTC CCATTGAATG GCTTTTTACG TTCTGAAATT 
TCACGTACTT CCTCTGATGT GTCTGAGTCC TGCAAACCAT TCCCAATTAC ACCAGAGTTG 
CACTCTTCCC CGGAGTTCCT GCTAAAAACC AATCTGAATC CCGAGGTCAG CTGTATTGAA 
AACACATCGG AGAGCTGTTT GATAAAGCCC TCGGTATTTG ATAATGCATC TTTAACTTCT 
CCCCAGGACA GGTTCAGTTC TTCGAGTGTG CCATCTGAAG GAACGACCTC TAGCGCTTCA 
TGTTATATGT CAAGCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301226.1
CDS	744..3464
Translation	MPGMVLFRRRWSVGSDDLVLPAFFLFVLHCIWLVVLSVVLFGLPYSSEQSCSVTLVDHGRGYLGILVSCLICESAIMWLSMRGSILYTQPREAVQYVLYIRLAILLVELVYAVVGIAWLVQYYQPCPDITAKNLALGIVACNWLVIFSVCFTMMCTFDPTGRTFVKLKATRRRQRNLTTYTLRHRLEEGQASSWSRRLKFFMCCTRAKDTQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRSAILDQANNDVLAFLSGMPVTRNTKYLDLKNSTEMAMYKEVCYYMLFAMAAYGWPVYLLRKPACGLCRLVSTCSCNTSVSGSRLSQSVTVEEDNCCGCNVLAIRRQFLDRDLKEVQIVYTSCHDAVYETPFFVAVDHAKKKVVISIRGTLSPKDALTDLTGDSERLPVEEQHGTWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLSKGTMHYGLVIVGHSLGAGTAAILSFLLRPQYPSLQCYSYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLEVLQKSDKPKWRIIAGGTKCIPKSELPMDDEAPVSQGVTPSSSRLWLHPSDLSIALSASTPLFPPGRVIHVVHNHPPEMCCGQEEPTYSALWGDNKAFDEVIISPAMLNEHMPHVVMDGLNKVLENPHLISDLIAAQVGSRKASNEVHNSVPNPSSDPPFTPLCPDECFIVPKTPTETLSVQPRGSDTQRNGLSFKNSSTENTVTETPTAIPSPTKPVTVDTSSIRSNTKPEGASDDVQQSSTCPSQSNTTLSSPKLDFPTLAMPKTVPLNGFLRSEISRTSSDVSESCKPFPITPELHSSPEFLLKTNLNPEVSCIENTSESCLIKPSVFDNASLTSPQDRFSSSSVPSEGTTSSASCYMSSL

Target ORF information:

RefSeq Version	XM_009302951.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302951.2

ORF Insert Sequence:

ATGCCGGGCA TGGTACTGTT TCGGCGACGC TGGTCAGTCG GCAGTGATGA CCTGGTGTTG 
CCGGCTTTCT TCCTGTTTGT ACTACACTGC ATATGGCTGG TGGTTCTGTC CGTGGTTCTC 
TTTGGGCTGC CGTACAGTTC AGAGCAGTCA TGTTCAGTCA CGCTGGTGGA TCATGGTCGC 
GGTTACCTCG GCATCTTGGT CAGTTGTCTG ATCTGCGAGA GCGCCATCAT GTGGCTGAGC 
ATGAGGGGCA GCATCCTCTA CACACAACCC AGAGAGGCGG TGCAGTATGT GCTCTATATA 
CGCCTAGCCA TCCTGCTCGT GGAGCTGGTG TATGCAGTGG TGGGCATCGC CTGGTTGGTG 
CAGTACTACC AGCCCTGCCC AGACATCACC GCTAAGAACC TCGCCCTCGG CATTGTGGCA 
TGTAACTGGT TGGTCATCTT CAGTGTGTGT TTCACCATGA TGTGCACCTT TGACCCCACT 
GGACGGACCT TTGTCAAGCT GAAAGCAACT CGTCGACGTC AGCGTAACCT TACAACATAC 
ACACTCAGAC ACAGGCTAGA AGAGGGTCAA GCCAGCAGCT GGAGCAGAAG ACTCAAATTC 
TTCATGTGCT GCACCAGAGC AAAAGACACA CAATCTGACG CTTACTCTGA GGTTGCCAGC 
TTGTTCGCCG AGTTTTTCCG GGATCTGGAT ATCGTGCCGA GTGACATCAT TGCTGGTTTG 
GTGCTGCTCC GACAGAGACA ACGGGCTAAA AGATCAGCCA TCTTGGATCA GGCAAACAAT 
GATGTTTTAG CCTTTCTGTC TGGGATGCCT GTGACACGCA ACACTAAATA CTTGGACCTC 
AAGAACTCGA CTGAGATGGC TATGTATAAG GAAGTGTGCT ACTACATGTT GTTTGCCATG 
GCGGCATATG GCTGGCCCGT TTATCTTTTG AGAAAACCAG CATGTGGACT CTGCAGACTT 
GTCAGCACCT GCTCCTGCAA TACTTCAGTT TCAGGCTCTC GCCTCTCTCA ATCCGTCACC 
GTTGAGGAAG ATAACTGTTG CGGCTGTAAC GTTCTTGCCA TCCGACGCCA GTTTCTAGAC 
CGAGACCTCA AAGAAGTCCA GATTGTCTAC ACATCCTGCC ATGACGCGGT TTATGAGACG 
CCCTTCTTTG TAGCAGTGGA TCACGCAAAG AAGAAAGTCG TGATCAGCAT CAGAGGAACG 
TTGTCTCCTA AGGACGCTTT AACAGATCTC ACTGGCGATT CGGAGCGTTT GCCTGTCGAG 
GAGCAGCACG GCACGTGGCT CGGACACAAG GGAATGGTTT ATTCAGCCGA ATACATCAAG 
AAGAAACTGG AGCAAGAGAT GATCCTCTCT CAAGCCTTTG GGAGAGATTT GAGTAAAGGG 
ACGATGCACT ACGGGCTGGT GATAGTGGGT CATTCTCTGG GAGCGGGAAC AGCCGCCATC 
CTGTCCTTCC TCCTTCGGCC GCAGTATCCT TCACTGCAAT GCTATTCCTA CTCTCCTCCC 
GGCGGCCTGC TTAGTGAGGA TGCAATGGAG TACTCGAAAG AGTTTGTCAC CTCAGTGGTG 
TTGGGAAAAG ATCTGGTGCC CAGGATCGGC CTCTCTCAGC TTGAGGGTTT CCGTCGTCAC 
CTGCTTGAAG TCTTACAGAA GAGCGATAAA CCAAAGTGGA GGATCATTGC TGGAGGAACA 
AAGTGCATCC CTAAATCGGA GCTGCCCATG GATGACGAAG CCCCTGTATC TCAGGGCGTA 
ACACCCTCCA GTTCCCGCCT ATGGCTTCAT CCCAGTGATC TGAGCATCGC CCTGTCAGCC 
TCCACACCGC TGTTTCCCCC GGGACGGGTC ATTCATGTGG TGCACAATCA CCCCCCTGAG 
ATGTGTTGTG GTCAGGAAGA GCCCACGTAC TCTGCTCTGT GGGGCGATAA CAAGGCCTTT 
GATGAGGTCA TCATTTCTCC AGCCATGCTG AATGAACACA TGCCCCATGT GGTGATGGAT 
GGCCTCAACA AGGTGCTGGA AAACCCACAT TTAATTTCAG ACCTGATTGC TGCACAGGTT 
GGAAGCCGGA AAGCTAGCAA TGAAGTGCAT AATTCTGTCC CTAACCCATC CTCAGATCCC 
CCTTTTACAC CACTGTGTCC AGATGAATGC TTTATAGTTC CCAAAACTCC CACTGAAACA 
CTTTCTGTTC AACCACGGGG TTCAGACACT CAAAGGAACG GTTTATCATT CAAAAATTCG 
TCCACTGAAA ATACTGTAAC AGAAACTCCC ACTGCGATCC CATCACCCAC AAAGCCTGTT 
ACTGTAGATA CATCTTCCAT TAGGTCAAAT ACAAAACCTG AAGGCGCTTC TGATGACGTT 
CAGCAATCAT CAACATGTCC ATCTCAGTCC AACACTACAT TGAGCTCACC GAAGTTAGAC 
TTTCCAACAT TAGCAATGCC CAAAACGGTC CCATTGAATG GCTTTTTACG TTCTGAAATT 
TCACGTACTT CCTCTGATGT GTCTGAGTCC TGCAAACCAT TCCCAATTAC ACCAGAGTTG 
CACTCTTCCC CGGAGTTCCT GCTAAAAACC AATCTGAATC CCGAGGTCAG CTGTATTGAA 
AACACATCGG AGAGCTGTTT GATAAAGCCC TCGGTATTTG ATAATGCATC TTTAACTTCT 
CCCCAGGACA GGTTCAGTTC TTCGAGTGTG CCATCTGAAG GAACGACCTC TAGCGCTTCA 
TGTTATATGT CAAGCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_009302952.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301227.1 Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa45734
Clone ID Related Accession (Same CDS sequence)	XM_009302952.2
Accession Version	XM_009302952.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sn1-specific diacylglycerol lipase alpha isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGCA TGGTACTGTT TCGGCGACGC TGGTCAGTCG GCAGTGATGA CCTGGTGTTG 
CCGGCTTTCT TCCTGTTTGT ACTACACTGC ATATGGCTGG TGGTTCTGTC CGTGGTTCTC 
TTTGGGCTGC CGTACAGTTC AGAGCAGTCA TGTTCAGTCA CGCTGGTGGA TCATGGTCGC 
GGTTACCTCG GCATCTTGGT CAGTTGTCTG ATCTGCGAGA GCGCCATCAT GTGGCTGAGC 
ATGAGGGGCA GCATCCTCTA CACACAACCC AGAGAGGCGG TGCAGTATGT GCTCTATATA 
CGCCTAGCCA TCCTGCTCGT GGAGCTGGTG TATGCAGTGG TGGGCATCGC CTGGTTGGTG 
CAGTACTACC AGCCCTGCCC AGACATCACC GCTAAGAACC TCGCCCTCGG CATTGTGGCA 
TGTAACTGGT TGGTCATCTT CAGTGTGTGT TTCACCATGA TGTGCACCTT TGACCCCACT 
GGACGGACCT TTGTCAAGCT GAAAGCAACT CGTCGACGTC AGCGTAACCT TACAACATAC 
ACACTCAGAC ACAGGCTAGA AGAGGGTCAA GCCAGCAGCT GGAGCAGAAG ACTCAAATTC 
TTCATGTGCT GCACCAGAGC AAAAGACACA CAATCTGACG CTTACTCTGA GGTTGCCAGC 
TTGTTCGCCG AGTTTTTCCG GGATCTGGAT ATCGTGCCGA GTGACATCAT TGCTGGTTTG 
GTGCTGCTCC GACAGAGACA ACGGGCTAAA AGATCAGCCA TCTTGGATCA GGCAAACAAT 
GATGTTTTAG CCTTTCTGTC TGGGATGCCT GTGACACGCA ACACTAAATA CTTGGACCTC 
AAGAACTCGA CTGAGATGGC TATGTATAAG GAAGTGTGCT ACTACATGTT GTTTGCCATG 
GCGGCATATG GCTGGCCCGT TTATCTTTTG AGAAAACCAG CATGTGGACT CTGCAGACTT 
GTCAGCACCT GCTCCTGCAA TACTTCAGTT TCAGGCTCTC GCCTCTCTCA ATCCGTCACC 
GTTGAGGAAG ATAACTGTTG CGGCTGTAAC GTTCTTGCCA TCCGACGCCA GTTTCTAGAC 
CGAGACCTCA AAGAAGTCCA GATTGTCTAC ACATCCTGCC ATGACGCGGT TTATGAGACG 
CCCTTCTTTG TAGCAGTGGA TCACGCAAAG AAGAAAGTCG TGATCAGCAT CAGAGGAACG 
TTGTCTCCTA AGGACGCTTT AACAGATCTC ACTGGCGATT CGGAGCGTTT GCCTGTCGAG 
GAGCAGCACG GCACGTGGCT CGGACACAAG GGAATGGTTT ATTCAGCCGA ATACATCAAG 
AAGAAACTGG AGCAAGAGAT GATCCTCTCT CAAGCCTTTG GGAGAGATTT GAGTAAAGGG 
ACGATGCACT ACGGGCTGGT GATAGTGGGT CATTCTCTGG GAGCGGGAAC AGCCGCCATC 
CTGTCCTTCC TCCTTCGGCC GCAGTATCCT TCACTGCAAT GCTATTCCTA CTCTCCTCCC 
GGCGGCCTGC TTAGTGAGGA TGCAATGGAG TACTCGAAAG AGTTTGTCAC CTCAGTGGTG 
TTGGGAAAAG ATCTGGTGCC CAGGATCGGC CTCTCTCAGC TTGAGGGTTT CCGTCGTCAC 
CTGCTTGAAG TCTTACAGAA GAGCGATAAA CCAAAGTGGA GGATCATTGC TGGAGGAACA 
AAGTGCATCC CTAAATCGGA GCTGCCCATG GATGACGAAG CCCCTGTATC TCAGGGCGTA 
ACACCCTCCA GTTCCCGCCT ATGGCTTCAT CCCAGTGATC TGAGCATCGC CCTGTCAGCC 
TCCACACCGC TGTTTCCCCC GGGACGGGTC ATTCATGTGG TGCACAATCA CCCCCCTGAG 
ATGTGTTGTG GTCAGGAAGA GCCCACGTAC TCTGCTCTGT GGGGCGATAA CAAGGCCTTT 
GATGAGGTCA TCATTTCTCC AGCCATGCTG AATGAACACA TGCCCCATGT GGTGATGGAT 
GGCCTCAACA AGGTGTTGGA AAACTACAAT AAAGGGAAGA CAGCTTTACT GTCTGCTGCT 
AAGATAATGG TCAGTCCAAC AGAAGTGGAC CTGAATCCAG AAACCATCTT CCTGGACACC 
TCAAATTCGC CACAGCCTAC GCCAGCCACA CACCACCGCA GAAACAGCAG TGTCCGAGGT 
ACTGTGTACG GTACTATTCA GTTCCCCCCT CGTCCTCTCT GGTTTAGTAG AAACGCAGAG 
TCTAGATGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301227.1
CDS	743..2974
Translation	MPGMVLFRRRWSVGSDDLVLPAFFLFVLHCIWLVVLSVVLFGLPYSSEQSCSVTLVDHGRGYLGILVSCLICESAIMWLSMRGSILYTQPREAVQYVLYIRLAILLVELVYAVVGIAWLVQYYQPCPDITAKNLALGIVACNWLVIFSVCFTMMCTFDPTGRTFVKLKATRRRQRNLTTYTLRHRLEEGQASSWSRRLKFFMCCTRAKDTQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRSAILDQANNDVLAFLSGMPVTRNTKYLDLKNSTEMAMYKEVCYYMLFAMAAYGWPVYLLRKPACGLCRLVSTCSCNTSVSGSRLSQSVTVEEDNCCGCNVLAIRRQFLDRDLKEVQIVYTSCHDAVYETPFFVAVDHAKKKVVISIRGTLSPKDALTDLTGDSERLPVEEQHGTWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLSKGTMHYGLVIVGHSLGAGTAAILSFLLRPQYPSLQCYSYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLEVLQKSDKPKWRIIAGGTKCIPKSELPMDDEAPVSQGVTPSSSRLWLHPSDLSIALSASTPLFPPGRVIHVVHNHPPEMCCGQEEPTYSALWGDNKAFDEVIISPAMLNEHMPHVVMDGLNKVLENYNKGKTALLSAAKIMVSPTEVDLNPETIFLDTSNSPQPTPATHHRRNSSVRGTVYGTIQFPPRPLWFSRNAESRC

Target ORF information:

RefSeq Version	XM_009302952.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302952.2

ORF Insert Sequence:

ATGCCGGGCA TGGTACTGTT TCGGCGACGC TGGTCAGTCG GCAGTGATGA CCTGGTGTTG 
CCGGCTTTCT TCCTGTTTGT ACTACACTGC ATATGGCTGG TGGTTCTGTC CGTGGTTCTC 
TTTGGGCTGC CGTACAGTTC AGAGCAGTCA TGTTCAGTCA CGCTGGTGGA TCATGGTCGC 
GGTTACCTCG GCATCTTGGT CAGTTGTCTG ATCTGCGAGA GCGCCATCAT GTGGCTGAGC 
ATGAGGGGCA GCATCCTCTA CACACAACCC AGAGAGGCGG TGCAGTATGT GCTCTATATA 
CGCCTAGCCA TCCTGCTCGT GGAGCTGGTG TATGCAGTGG TGGGCATCGC CTGGTTGGTG 
CAGTACTACC AGCCCTGCCC AGACATCACC GCTAAGAACC TCGCCCTCGG CATTGTGGCA 
TGTAACTGGT TGGTCATCTT CAGTGTGTGT TTCACCATGA TGTGCACCTT TGACCCCACT 
GGACGGACCT TTGTCAAGCT GAAAGCAACT CGTCGACGTC AGCGTAACCT TACAACATAC 
ACACTCAGAC ACAGGCTAGA AGAGGGTCAA GCCAGCAGCT GGAGCAGAAG ACTCAAATTC 
TTCATGTGCT GCACCAGAGC AAAAGACACA CAATCTGACG CTTACTCTGA GGTTGCCAGC 
TTGTTCGCCG AGTTTTTCCG GGATCTGGAT ATCGTGCCGA GTGACATCAT TGCTGGTTTG 
GTGCTGCTCC GACAGAGACA ACGGGCTAAA AGATCAGCCA TCTTGGATCA GGCAAACAAT 
GATGTTTTAG CCTTTCTGTC TGGGATGCCT GTGACACGCA ACACTAAATA CTTGGACCTC 
AAGAACTCGA CTGAGATGGC TATGTATAAG GAAGTGTGCT ACTACATGTT GTTTGCCATG 
GCGGCATATG GCTGGCCCGT TTATCTTTTG AGAAAACCAG CATGTGGACT CTGCAGACTT 
GTCAGCACCT GCTCCTGCAA TACTTCAGTT TCAGGCTCTC GCCTCTCTCA ATCCGTCACC 
GTTGAGGAAG ATAACTGTTG CGGCTGTAAC GTTCTTGCCA TCCGACGCCA GTTTCTAGAC 
CGAGACCTCA AAGAAGTCCA GATTGTCTAC ACATCCTGCC ATGACGCGGT TTATGAGACG 
CCCTTCTTTG TAGCAGTGGA TCACGCAAAG AAGAAAGTCG TGATCAGCAT CAGAGGAACG 
TTGTCTCCTA AGGACGCTTT AACAGATCTC ACTGGCGATT CGGAGCGTTT GCCTGTCGAG 
GAGCAGCACG GCACGTGGCT CGGACACAAG GGAATGGTTT ATTCAGCCGA ATACATCAAG 
AAGAAACTGG AGCAAGAGAT GATCCTCTCT CAAGCCTTTG GGAGAGATTT GAGTAAAGGG 
ACGATGCACT ACGGGCTGGT GATAGTGGGT CATTCTCTGG GAGCGGGAAC AGCCGCCATC 
CTGTCCTTCC TCCTTCGGCC GCAGTATCCT TCACTGCAAT GCTATTCCTA CTCTCCTCCC 
GGCGGCCTGC TTAGTGAGGA TGCAATGGAG TACTCGAAAG AGTTTGTCAC CTCAGTGGTG 
TTGGGAAAAG ATCTGGTGCC CAGGATCGGC CTCTCTCAGC TTGAGGGTTT CCGTCGTCAC 
CTGCTTGAAG TCTTACAGAA GAGCGATAAA CCAAAGTGGA GGATCATTGC TGGAGGAACA 
AAGTGCATCC CTAAATCGGA GCTGCCCATG GATGACGAAG CCCCTGTATC TCAGGGCGTA 
ACACCCTCCA GTTCCCGCCT ATGGCTTCAT CCCAGTGATC TGAGCATCGC CCTGTCAGCC 
TCCACACCGC TGTTTCCCCC GGGACGGGTC ATTCATGTGG TGCACAATCA CCCCCCTGAG 
ATGTGTTGTG GTCAGGAAGA GCCCACGTAC TCTGCTCTGT GGGGCGATAA CAAGGCCTTT 
GATGAGGTCA TCATTTCTCC AGCCATGCTG AATGAACACA TGCCCCATGT GGTGATGGAT 
GGCCTCAACA AGGTGTTGGA AAACTACAAT AAAGGGAAGA CAGCTTTACT GTCTGCTGCT 
AAGATAATGG TCAGTCCAAC AGAAGTGGAC CTGAATCCAG AAACCATCTT CCTGGACACC 
TCAAATTCGC CACAGCCTAC GCCAGCCACA CACCACCGCA GAAACAGCAG TGTCCGAGGT 
ACTGTGTACG GTACTATTCA GTTCCCCCCT CGTCCTCTCT GGTTTAGTAG AAACGCAGAG 
TCTAGATGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_009302951.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301226.2 Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa144786
Clone ID Related Accession (Same CDS sequence)	XM_009302951.3
Accession Version	XM_009302951.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sn1-specific diacylglycerol lipase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394675.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302951.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGCA TGGTACTGTT TCGGCGACGC TGGTCAGTCG GCAGTGATGA CCTTGTGTTG 
CCGGCTTTCT TCCTGTTCGT ACTACACTGC ATATGGCTGG TGGTTCTGTC TGTGGTTCTC 
TTTGGGCTGC CGTACAGTTC AGAGCAGTCA TGTTCAGTCA CGCTGGTGGA TCATGGTCGC 
GGTTACCTCG GCATCTTGGT CAGTTGTCTG ATCTGCGAGA GCGCCATCAT GTGGCTGAGC 
ATGAGGGGCA GCATCCTCTA CACACAACCC AGAGAGGCAG TGCAGTATGT GCTCTATATA 
CGCCTAGCCA TCCTGCTCGT GGAGCTGGTG TATGCAGTGG TGGGCATCGC CTGGTTGGTG 
CAGTACTACC AGCCCTGCCC AGACATCACC GCTAAGAACC TCGCCCTCGG GATTGTGGCA 
TGTAACTGGT TGGTCATCTT CAGTGTGTGT TTCACCATGA TGTGCACCTT TGACCCCACC 
GGACGGACCT TTGTCAAGCT GAAAGCAACT CGTCGACGTC AGCGTAACCT TACAACATAC 
ACACTCAGAC ACAGGCTAGA AGAGGGTCAA GCCAGCAGCT GGAGCAGAAG ACTCAAATTC 
TTCATGTGCT GCACCAGAGC AAAAGACACA CAATCTGACG CGTACTCTGA GGTTGCCAGC 
TTGTTCGCCG AGTTTTTCAG AGATCTGGAT ATCGTGCCGA GTGACATCAT TGCTGGTTTG 
GTGCTGCTCC GACAGAGACA ACGGGCTAAA AGATCAGCCA TCTTGGATCA GGCAAACAAT 
GATGTTTTAG CCTTTCTGTC TGGGATGCCT GTGACACGCA ACACTAAATA CTTGGACCTC 
AAGAACTCGA CTGAGATGGC CATGTATAAG GAAGTGTGCT ACTACATGTT GTTTGCCATG 
GCGGCATATG GCTGGCCCGT TTATCTTTTG AGAAAACCAG CATGTGGACT CTGCAGACTT 
GTCAGCACCT GCTCCTGCAA TACTTCAGTT TCAAGCTCTC GCCTCTCTCA GTCCGTCACC 
GTTGAGGAAG ATAACTGCTG CGGCTGTAAC GTTCTTGCCA TCCGACGCCA GTTTCTAGAC 
CGAGACCTCA AAGAAGTCCA GATTGTCTAC ACATCCTGCC ATGACGCGGT TTATGAGACG 
CCCTTCTTTG TAGCAGTGGA TCACGCAAAG AAGAAAGTCG TGATCAGCAT CAGAGGAACG 
TTGTCTCCTA AGGACGCTTT AACAGATCTC ACTGGCGATT CGGAGCGTTT GCCTGTCGAG 
GAGCAGCACG GCACGTGGCT CGGACACAAG GGAATGGTTT ATTCAGCCGA ATACATCAAG 
AAGAAACTGG AGCAAGAGAT GATCCTCTCT CAAGCCTTTG GGAGAGATTT GAGTAAAGGG 
ACGATGCACT ACGGGCTGGT GATTGTGGGT CATTCTCTGG GAGCGGGAAC AGCCGCCATC 
CTGTCCTTCC TCCTTCGGCC GCAGTATCCT TCACTGCAAT GCTATTCCTA CTCTCCTCCC 
GGCGGCCTGC TTAGTGAGGA TGCAATGGAG TACTCGAAAG AGTTCGTCAC CTCAGTGGTG 
TTGGGAAAAG ATCTGGTGCC CAGGATCGGC CTCTCTCAGC TTGAGGGTTT CCGTCGTCAC 
CTGCTTGAAG TCTTACAGAA GAGCAATAAA CCAAAGTGGA GGATCATTGC TGGAGGAACA 
AAGTGCATCC CTAAATCGGA GCTGCCCATG GATGACGAAG CCCCTGTATC TCAGGGCGTA 
ACACCCTCCA GTTCCCGCCT ATGGCTTCAT CCCAGTGATC TGAGCATCGC CCTGTCAGCC 
TCCACACCGC TGTTTCCCCC GGGTCGGGTC ATTCATGTGG TGCACAATCA CCCCCCTGAG 
ATGTGTTGTG GTCAGGAAGA GCCCACCTAC TCTGCTCTGT GGGGCGATAA CAAGGCCTTT 
GATGAGGTCA TCATTTCTCC AGCCATGCTG AATGAACACA TGCCCCATGT GGTGATGGAT 
GGCCTCAACA AGGTGCTGGA AAACCCACAT TTAATTTCAG ACCTGATTGC TGCACAGGTT 
GGAAGCCGGA AAGCTAGCAA TGAAGTGCAT AATTCTGTCC CTAACCCATC CTCAGATCCC 
CCTTTTACAC CACTGTGTCC AGATGAATGC TTTATAGTTC CCAAAACTCC CACTGAAACA 
CTTTCTGTTC AACCACGGGG TTCAGACACT CAAAGGAACG GTTTATCATT CAAAAATTCG 
TCCACTGAAA ATACTGTAAC AGAAACTCCC ACTGCGATCC CATCACCCAC AAAGCCTGTT 
ACTGTAGATA CATCTTCCAT TAGGTCAAAT ACAAAACCTG AAGGCGCTTC TGATGACGTT 
CAGCAATCAT CAACATGTCC ATCTCAGTCC AACACTACAT TGAGCTCACC GAAGTTAGAC 
TTTCCAACAT TAGCAATGCC CAAAACGGTC CCATTGAATG GCTTTTTACG TTCTGAAATT 
TCACGTACTT CCTCTGATGT GTCTGAGTCC TGCAAACCAT TCCCAATTAC ACCAGAGTTG 
CACTCTTCCC CGGAGTTCCT GCTAAAAACC AATCTGAATC CCGAGGTCAG CTGTATTGAA 
AACACATCGG AGAGCTGTTT GATAAAGCCC TCGGTATTTG ATAATGCATC TTTAACTTCT 
CCCCAGGACA GGTTCAGTTC TTCGAGTGTG CCATCTGAAG GAACGACCTC TAGCGCTTCA 
TGTTATATGT CAAGCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301226.2
CDS	749..3469
Translation	MPGMVLFRRRWSVGSDDLVLPAFFLFVLHCIWLVVLSVVLFGLPYSSEQSCSVTLVDHGRGYLGILVSCLICESAIMWLSMRGSILYTQPREAVQYVLYIRLAILLVELVYAVVGIAWLVQYYQPCPDITAKNLALGIVACNWLVIFSVCFTMMCTFDPTGRTFVKLKATRRRQRNLTTYTLRHRLEEGQASSWSRRLKFFMCCTRAKDTQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRSAILDQANNDVLAFLSGMPVTRNTKYLDLKNSTEMAMYKEVCYYMLFAMAAYGWPVYLLRKPACGLCRLVSTCSCNTSVSSSRLSQSVTVEEDNCCGCNVLAIRRQFLDRDLKEVQIVYTSCHDAVYETPFFVAVDHAKKKVVISIRGTLSPKDALTDLTGDSERLPVEEQHGTWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLSKGTMHYGLVIVGHSLGAGTAAILSFLLRPQYPSLQCYSYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLEVLQKSNKPKWRIIAGGTKCIPKSELPMDDEAPVSQGVTPSSSRLWLHPSDLSIALSASTPLFPPGRVIHVVHNHPPEMCCGQEEPTYSALWGDNKAFDEVIISPAMLNEHMPHVVMDGLNKVLENPHLISDLIAAQVGSRKASNEVHNSVPNPSSDPPFTPLCPDECFIVPKTPTETLSVQPRGSDTQRNGLSFKNSSTENTVTETPTAIPSPTKPVTVDTSSIRSNTKPEGASDDVQQSSTCPSQSNTTLSSPKLDFPTLAMPKTVPLNGFLRSEISRTSSDVSESCKPFPITPELHSSPEFLLKTNLNPEVSCIENTSESCLIKPSVFDNASLTSPQDRFSSSSVPSEGTTSSASCYMSSL

Target ORF information:

RefSeq Version	XM_009302951.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302951.3

ORF Insert Sequence:

ATGCCGGGCA TGGTACTGTT TCGGCGACGC TGGTCAGTCG GCAGTGATGA CCTTGTGTTG 
CCGGCTTTCT TCCTGTTCGT ACTACACTGC ATATGGCTGG TGGTTCTGTC TGTGGTTCTC 
TTTGGGCTGC CGTACAGTTC AGAGCAGTCA TGTTCAGTCA CGCTGGTGGA TCATGGTCGC 
GGTTACCTCG GCATCTTGGT CAGTTGTCTG ATCTGCGAGA GCGCCATCAT GTGGCTGAGC 
ATGAGGGGCA GCATCCTCTA CACACAACCC AGAGAGGCAG TGCAGTATGT GCTCTATATA 
CGCCTAGCCA TCCTGCTCGT GGAGCTGGTG TATGCAGTGG TGGGCATCGC CTGGTTGGTG 
CAGTACTACC AGCCCTGCCC AGACATCACC GCTAAGAACC TCGCCCTCGG GATTGTGGCA 
TGTAACTGGT TGGTCATCTT CAGTGTGTGT TTCACCATGA TGTGCACCTT TGACCCCACC 
GGACGGACCT TTGTCAAGCT GAAAGCAACT CGTCGACGTC AGCGTAACCT TACAACATAC 
ACACTCAGAC ACAGGCTAGA AGAGGGTCAA GCCAGCAGCT GGAGCAGAAG ACTCAAATTC 
TTCATGTGCT GCACCAGAGC AAAAGACACA CAATCTGACG CGTACTCTGA GGTTGCCAGC 
TTGTTCGCCG AGTTTTTCAG AGATCTGGAT ATCGTGCCGA GTGACATCAT TGCTGGTTTG 
GTGCTGCTCC GACAGAGACA ACGGGCTAAA AGATCAGCCA TCTTGGATCA GGCAAACAAT 
GATGTTTTAG CCTTTCTGTC TGGGATGCCT GTGACACGCA ACACTAAATA CTTGGACCTC 
AAGAACTCGA CTGAGATGGC CATGTATAAG GAAGTGTGCT ACTACATGTT GTTTGCCATG 
GCGGCATATG GCTGGCCCGT TTATCTTTTG AGAAAACCAG CATGTGGACT CTGCAGACTT 
GTCAGCACCT GCTCCTGCAA TACTTCAGTT TCAAGCTCTC GCCTCTCTCA GTCCGTCACC 
GTTGAGGAAG ATAACTGCTG CGGCTGTAAC GTTCTTGCCA TCCGACGCCA GTTTCTAGAC 
CGAGACCTCA AAGAAGTCCA GATTGTCTAC ACATCCTGCC ATGACGCGGT TTATGAGACG 
CCCTTCTTTG TAGCAGTGGA TCACGCAAAG AAGAAAGTCG TGATCAGCAT CAGAGGAACG 
TTGTCTCCTA AGGACGCTTT AACAGATCTC ACTGGCGATT CGGAGCGTTT GCCTGTCGAG 
GAGCAGCACG GCACGTGGCT CGGACACAAG GGAATGGTTT ATTCAGCCGA ATACATCAAG 
AAGAAACTGG AGCAAGAGAT GATCCTCTCT CAAGCCTTTG GGAGAGATTT GAGTAAAGGG 
ACGATGCACT ACGGGCTGGT GATTGTGGGT CATTCTCTGG GAGCGGGAAC AGCCGCCATC 
CTGTCCTTCC TCCTTCGGCC GCAGTATCCT TCACTGCAAT GCTATTCCTA CTCTCCTCCC 
GGCGGCCTGC TTAGTGAGGA TGCAATGGAG TACTCGAAAG AGTTCGTCAC CTCAGTGGTG 
TTGGGAAAAG ATCTGGTGCC CAGGATCGGC CTCTCTCAGC TTGAGGGTTT CCGTCGTCAC 
CTGCTTGAAG TCTTACAGAA GAGCAATAAA CCAAAGTGGA GGATCATTGC TGGAGGAACA 
AAGTGCATCC CTAAATCGGA GCTGCCCATG GATGACGAAG CCCCTGTATC TCAGGGCGTA 
ACACCCTCCA GTTCCCGCCT ATGGCTTCAT CCCAGTGATC TGAGCATCGC CCTGTCAGCC 
TCCACACCGC TGTTTCCCCC GGGTCGGGTC ATTCATGTGG TGCACAATCA CCCCCCTGAG 
ATGTGTTGTG GTCAGGAAGA GCCCACCTAC TCTGCTCTGT GGGGCGATAA CAAGGCCTTT 
GATGAGGTCA TCATTTCTCC AGCCATGCTG AATGAACACA TGCCCCATGT GGTGATGGAT 
GGCCTCAACA AGGTGCTGGA AAACCCACAT TTAATTTCAG ACCTGATTGC TGCACAGGTT 
GGAAGCCGGA AAGCTAGCAA TGAAGTGCAT AATTCTGTCC CTAACCCATC CTCAGATCCC 
CCTTTTACAC CACTGTGTCC AGATGAATGC TTTATAGTTC CCAAAACTCC CACTGAAACA 
CTTTCTGTTC AACCACGGGG TTCAGACACT CAAAGGAACG GTTTATCATT CAAAAATTCG 
TCCACTGAAA ATACTGTAAC AGAAACTCCC ACTGCGATCC CATCACCCAC AAAGCCTGTT 
ACTGTAGATA CATCTTCCAT TAGGTCAAAT ACAAAACCTG AAGGCGCTTC TGATGACGTT 
CAGCAATCAT CAACATGTCC ATCTCAGTCC AACACTACAT TGAGCTCACC GAAGTTAGAC 
TTTCCAACAT TAGCAATGCC CAAAACGGTC CCATTGAATG GCTTTTTACG TTCTGAAATT 
TCACGTACTT CCTCTGATGT GTCTGAGTCC TGCAAACCAT TCCCAATTAC ACCAGAGTTG 
CACTCTTCCC CGGAGTTCCT GCTAAAAACC AATCTGAATC CCGAGGTCAG CTGTATTGAA 
AACACATCGG AGAGCTGTTT GATAAAGCCC TCGGTATTTG ATAATGCATC TTTAACTTCT 
CCCCAGGACA GGTTCAGTTC TTCGAGTGTG CCATCTGAAG GAACGACCTC TAGCGCTTCA 
TGTTATATGT CAAGCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_692781.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_697873.6 Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa144785
Clone ID Related Accession (Same CDS sequence)	XM_692781.8
Accession Version	XM_692781.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394675.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_692781.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGCA TGGTACTGTT TCGGCGACGC TGGTCAGTCG GCAGTGATGA CCTTGTGTTG 
CCGGCTTTCT TCCTGTTCGT ACTACACTGC ATATGGCTGG TGGTTCTGTC TGTGGTTCTC 
TTTGGGCTGC CGTACAGTTC AGAGCAGTCA TGTTCAGTCA CGCTGGTGGA TCATGGTCGC 
GGTTACCTCG GCATCTTGGT CAGTTGTCTG ATCTGCGAGA GCGCCATCAT GTGGCTGAGC 
ATGAGGGGCA GCATCCTCTA CACACAACCC AGAGAGGCAG TGCAGTATGT GCTCTATATA 
CGCCTAGCCA TCCTGCTCGT GGAGCTGGTG TATGCAGTGG TGGGCATCGC CTGGTTGGTG 
CAGTACTACC AGCCCTGCCC AGACATCACC GCTAAGAACC TCGCCCTCGG GATTGTGGCA 
TGTAACTGGT TGGTCATCTT CAGTGTGTGT TTCACCATGA TGTGCACCTT TGACCCCACC 
GGACGGACCT TTGTCAAGCT GAAAGCAACT CGTCGACGTC AGCGTAACCT TACAACATAC 
ACACTCAGAC ACAGGCTAGA AGAGGGTCAA GCCAGCAGCT GGAGCAGAAG ACTCAAATTC 
TTCATGTGCT GCACCAGAGC AAAAGACACA CAATCTGACG CGTACTCTGA GGTTGCCAGC 
TTGTTCGCCG AGTTTTTCAG AGATCTGGAT ATCGTGCCGA GTGACATCAT TGCTGGTTTG 
GTGCTGCTCC GACAGAGACA ACGGGCTAAA AGATCAGCCA TCTTGGATCA GGCAAACAAT 
GATGTTTTAG CCTTTCTGTC TGGGATGCCT GTGACACGCA ACACTAAATA CTTGGACCTC 
AAGAACTCGA CTGAGATGGC CATGTATAAG GAAGTGTGCT ACTACATGTT GTTTGCCATG 
GCGGCATATG GCTGGCCCGT TTATCTTTTG AGAAAACCAG CATGTGGACT CTGCAGACTT 
GTCAGCACCT GCTCCTGCAA TACTTCAGTT TCAAGCTCTC GCCTCTCTCA GTCCGTCACC 
GTTGAGGAAG ATAACTGCTG CGGCTGTAAC GTTCTTGCCA TCCGACGCCA GTTTCTAGAC 
CGAGACCTCA AAGAAGTCCA GATTGTCTAC ACATCCTGCC ATGACGCGGT TTATGAGACG 
CCCTTCTTTG TAGCAGTGGA TCACGCAAAG AAGAAAGTCG TGATCAGCAT CAGAGGAACG 
TTGTCTCCTA AGGACGCTTT AACAGATCTC ACTGGCGATT CGGAGCGTTT GCCTGTCGAG 
GAGCAGCACG GCACGTGGCT CGGACACAAG GGAATGGTTT ATTCAGCCGA ATACATCAAG 
AAGAAACTGG AGCAAGAGAT GATCCTCTCT CAAGCCTTTG GGAGAGATTT GAGTAAAGGG 
ACGATGCACT ACGGGCTGGT GATTGTGGGT CATTCTCTGG GAGCGGGAAC AGCCGCCATC 
CTGTCCTTCC TCCTTCGGCC GCAGTATCCT TCACTGCAAT GCTATTCCTA CTCTCCTCCC 
GGCGGCCTGC TTAGTGAGGA TGCAATGGAG TACTCGAAAG AGTTCGTCAC CTCAGTGGTG 
TTGGGAAAAG ATCTGGTGCC CAGGATCGGC CTCTCTCAGC TTGAGGGTTT CCGTCGTCAC 
CTGCTTGAAG TCTTACAGAA GAGCAATAAA CCAAAGTGGA GGATCATTGC TGGAGGAACA 
AAGTGCATCC CTAAATCGGA GCTGCCCATG GATGACGAAG CCCCTGTATC TCAGGGCGTA 
ACACCCTCCA GTTCCCGCCT ATGGCTTCAT CCCAGTGATC TGAGCATCGC CCTGTCAGCC 
TCCACACCGC TGTTTCCCCC GGGTCGGGTC ATTCATGTGG TGCACAATCA CCCCCCTGAG 
ATGTGTTGTG GTCAGGAAGA GCCCACCTAC TCTGCTCTGT GGGGCGATAA CAAGGCCTTT 
GATGAGGTCA TCATTTCTCC AGCCATGCTG AATGAACACA TGCCCCATGT GGTGATGGAT 
GGCCTCAACA AGGTGTTGGA AAACTACAAT AAAGGGAAGA CAGCTTTACT GTCTGCTGCT 
AAGATAATGG TCAGTCCAAC AGAAGTGGAC CTGAATCCAG AAACCATCTT CCTGGACACC 
TCAAATTCGC CACAGCCTAC GCCAGCCACA CACCACCGCA GAAACAGCAG TGTCCGTTCC 
TGCGCCCGGT CTGAGATCTC CCTTGATGGC TTCTCTGAGT GTCCGCCTCC TCCTGTCCCT 
ATTGTGCTCA CCGGAGCCCG TGAGCGACTG GCTGTGGAGC TGCGGGAGCG AAAAGCCCCC 
TTGGCCATCA TGGAAAGCCT GTCCGATGCC GAATCCGTCT ACAGCCTGGA CTCTCGCCGA 
TCATCTGCCG CCCTACGGGG CTCACCCTGC CTAGGGAGCC TACCTTTCCC ACTGGATGCC 
CCAATCCCAG AAGAGAATCC TTCATTGAGT TCTCGTACGG AGCTCCTAGC TGCTGAAGGG 
TTGACCCAAG ATTTGGGGGA GGCTGGACCA TACATTCCTA CACCTCCAGA GCCAGAAGAA 
CCCTCCAACG ACATCCCATA CAACAACGAA TACTTCACTC CAGAATTGAA GAGTACCACA 
GATCCAGAAA CAGATTCATC TGTGGTTCCC GACAGTCAAG ACTCCTACCG TGTAGCATCC 
CGGACTGGTT CTCGTCCAGA AAAGCTCAGG GAGTTTGAAG AAAAAGAGGT CAAAGTTGAT 
CTCTCCCAAA CTCTTCCCGC AGTTACTCCA CTATCCAACG GTACTCCCAG TCAGGCGGTC 
CTAGAGTTCG CCCAGTACCT AGACTCTCTG TTTAGGCTGG ACGGAAGCAG TTCGCCTCCT 
CTTGAGCTCT CGGATGCTGA GTCCGAGTCC GGCCGGGGAT CCTACAGTCA GGCTGAGGAA 
CACCAAGAGG AGCAGCGTGT CAAAAACGCG GATAAGCTAC TAGCTAGAGC CGCGCTAGAG 
CCCAATTTGG TTCCCAAGCC TCCTCGCACA TTTGCCGGCT CTGCTGACCC GTCCTCCGGC 
ATCTCTCTTT CTCCATCCTT CCCGCTCTCA TCTTCCGGAG AACTCAACGA TTTTTCTCCC 
GCTGATGGAG TGTTGCCTGT AAACCATACA TCTACGCTAC GAACTTCCGC TGCTAGTCCC 
AACCCCAAGG AAACTGCATT GTCGTCTATG GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_697873.6
CDS	743..3898
Translation	MPGMVLFRRRWSVGSDDLVLPAFFLFVLHCIWLVVLSVVLFGLPYSSEQSCSVTLVDHGRGYLGILVSCLICESAIMWLSMRGSILYTQPREAVQYVLYIRLAILLVELVYAVVGIAWLVQYYQPCPDITAKNLALGIVACNWLVIFSVCFTMMCTFDPTGRTFVKLKATRRRQRNLTTYTLRHRLEEGQASSWSRRLKFFMCCTRAKDTQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRSAILDQANNDVLAFLSGMPVTRNTKYLDLKNSTEMAMYKEVCYYMLFAMAAYGWPVYLLRKPACGLCRLVSTCSCNTSVSSSRLSQSVTVEEDNCCGCNVLAIRRQFLDRDLKEVQIVYTSCHDAVYETPFFVAVDHAKKKVVISIRGTLSPKDALTDLTGDSERLPVEEQHGTWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLSKGTMHYGLVIVGHSLGAGTAAILSFLLRPQYPSLQCYSYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLEVLQKSNKPKWRIIAGGTKCIPKSELPMDDEAPVSQGVTPSSSRLWLHPSDLSIALSASTPLFPPGRVIHVVHNHPPEMCCGQEEPTYSALWGDNKAFDEVIISPAMLNEHMPHVVMDGLNKVLENYNKGKTALLSAAKIMVSPTEVDLNPETIFLDTSNSPQPTPATHHRRNSSVRSCARSEISLDGFSECPPPPVPIVLTGARERLAVELRERKAPLAIMESLSDAESVYSLDSRRSSAALRGSPCLGSLPFPLDAPIPEENPSLSSRTELLAAEGLTQDLGEAGPYIPTPPEPEEPSNDIPYNNEYFTPELKSTTDPETDSSVVPDSQDSYRVASRTGSRPEKLREFEEKEVKVDLSQTLPAVTPLSNGTPSQAVLEFAQYLDSLFRLDGSSSPPLELSDAESESGRGSYSQAEEHQEEQRVKNADKLLARAALEPNLVPKPPRTFAGSADPSSGISLSPSFPLSSSGELNDFSPADGVLPVNHTSTLRTSAASPNPKETALSSMV

Target ORF information:

RefSeq Version	XM_692781.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_692781.8

ORF Insert Sequence:

ATGCCGGGCA TGGTACTGTT TCGGCGACGC TGGTCAGTCG GCAGTGATGA CCTTGTGTTG 
CCGGCTTTCT TCCTGTTCGT ACTACACTGC ATATGGCTGG TGGTTCTGTC TGTGGTTCTC 
TTTGGGCTGC CGTACAGTTC AGAGCAGTCA TGTTCAGTCA CGCTGGTGGA TCATGGTCGC 
GGTTACCTCG GCATCTTGGT CAGTTGTCTG ATCTGCGAGA GCGCCATCAT GTGGCTGAGC 
ATGAGGGGCA GCATCCTCTA CACACAACCC AGAGAGGCAG TGCAGTATGT GCTCTATATA 
CGCCTAGCCA TCCTGCTCGT GGAGCTGGTG TATGCAGTGG TGGGCATCGC CTGGTTGGTG 
CAGTACTACC AGCCCTGCCC AGACATCACC GCTAAGAACC TCGCCCTCGG GATTGTGGCA 
TGTAACTGGT TGGTCATCTT CAGTGTGTGT TTCACCATGA TGTGCACCTT TGACCCCACC 
GGACGGACCT TTGTCAAGCT GAAAGCAACT CGTCGACGTC AGCGTAACCT TACAACATAC 
ACACTCAGAC ACAGGCTAGA AGAGGGTCAA GCCAGCAGCT GGAGCAGAAG ACTCAAATTC 
TTCATGTGCT GCACCAGAGC AAAAGACACA CAATCTGACG CGTACTCTGA GGTTGCCAGC 
TTGTTCGCCG AGTTTTTCAG AGATCTGGAT ATCGTGCCGA GTGACATCAT TGCTGGTTTG 
GTGCTGCTCC GACAGAGACA ACGGGCTAAA AGATCAGCCA TCTTGGATCA GGCAAACAAT 
GATGTTTTAG CCTTTCTGTC TGGGATGCCT GTGACACGCA ACACTAAATA CTTGGACCTC 
AAGAACTCGA CTGAGATGGC CATGTATAAG GAAGTGTGCT ACTACATGTT GTTTGCCATG 
GCGGCATATG GCTGGCCCGT TTATCTTTTG AGAAAACCAG CATGTGGACT CTGCAGACTT 
GTCAGCACCT GCTCCTGCAA TACTTCAGTT TCAAGCTCTC GCCTCTCTCA GTCCGTCACC 
GTTGAGGAAG ATAACTGCTG CGGCTGTAAC GTTCTTGCCA TCCGACGCCA GTTTCTAGAC 
CGAGACCTCA AAGAAGTCCA GATTGTCTAC ACATCCTGCC ATGACGCGGT TTATGAGACG 
CCCTTCTTTG TAGCAGTGGA TCACGCAAAG AAGAAAGTCG TGATCAGCAT CAGAGGAACG 
TTGTCTCCTA AGGACGCTTT AACAGATCTC ACTGGCGATT CGGAGCGTTT GCCTGTCGAG 
GAGCAGCACG GCACGTGGCT CGGACACAAG GGAATGGTTT ATTCAGCCGA ATACATCAAG 
AAGAAACTGG AGCAAGAGAT GATCCTCTCT CAAGCCTTTG GGAGAGATTT GAGTAAAGGG 
ACGATGCACT ACGGGCTGGT GATTGTGGGT CATTCTCTGG GAGCGGGAAC AGCCGCCATC 
CTGTCCTTCC TCCTTCGGCC GCAGTATCCT TCACTGCAAT GCTATTCCTA CTCTCCTCCC 
GGCGGCCTGC TTAGTGAGGA TGCAATGGAG TACTCGAAAG AGTTCGTCAC CTCAGTGGTG 
TTGGGAAAAG ATCTGGTGCC CAGGATCGGC CTCTCTCAGC TTGAGGGTTT CCGTCGTCAC 
CTGCTTGAAG TCTTACAGAA GAGCAATAAA CCAAAGTGGA GGATCATTGC TGGAGGAACA 
AAGTGCATCC CTAAATCGGA GCTGCCCATG GATGACGAAG CCCCTGTATC TCAGGGCGTA 
ACACCCTCCA GTTCCCGCCT ATGGCTTCAT CCCAGTGATC TGAGCATCGC CCTGTCAGCC 
TCCACACCGC TGTTTCCCCC GGGTCGGGTC ATTCATGTGG TGCACAATCA CCCCCCTGAG 
ATGTGTTGTG GTCAGGAAGA GCCCACCTAC TCTGCTCTGT GGGGCGATAA CAAGGCCTTT 
GATGAGGTCA TCATTTCTCC AGCCATGCTG AATGAACACA TGCCCCATGT GGTGATGGAT 
GGCCTCAACA AGGTGTTGGA AAACTACAAT AAAGGGAAGA CAGCTTTACT GTCTGCTGCT 
AAGATAATGG TCAGTCCAAC AGAAGTGGAC CTGAATCCAG AAACCATCTT CCTGGACACC 
TCAAATTCGC CACAGCCTAC GCCAGCCACA CACCACCGCA GAAACAGCAG TGTCCGTTCC 
TGCGCCCGGT CTGAGATCTC CCTTGATGGC TTCTCTGAGT GTCCGCCTCC TCCTGTCCCT 
ATTGTGCTCA CCGGAGCCCG TGAGCGACTG GCTGTGGAGC TGCGGGAGCG AAAAGCCCCC 
TTGGCCATCA TGGAAAGCCT GTCCGATGCC GAATCCGTCT ACAGCCTGGA CTCTCGCCGA 
TCATCTGCCG CCCTACGGGG CTCACCCTGC CTAGGGAGCC TACCTTTCCC ACTGGATGCC 
CCAATCCCAG AAGAGAATCC TTCATTGAGT TCTCGTACGG AGCTCCTAGC TGCTGAAGGG 
TTGACCCAAG ATTTGGGGGA GGCTGGACCA TACATTCCTA CACCTCCAGA GCCAGAAGAA 
CCCTCCAACG ACATCCCATA CAACAACGAA TACTTCACTC CAGAATTGAA GAGTACCACA 
GATCCAGAAA CAGATTCATC TGTGGTTCCC GACAGTCAAG ACTCCTACCG TGTAGCATCC 
CGGACTGGTT CTCGTCCAGA AAAGCTCAGG GAGTTTGAAG AAAAAGAGGT CAAAGTTGAT 
CTCTCCCAAA CTCTTCCCGC AGTTACTCCA CTATCCAACG GTACTCCCAG TCAGGCGGTC 
CTAGAGTTCG CCCAGTACCT AGACTCTCTG TTTAGGCTGG ACGGAAGCAG TTCGCCTCCT 
CTTGAGCTCT CGGATGCTGA GTCCGAGTCC GGCCGGGGAT CCTACAGTCA GGCTGAGGAA 
CACCAAGAGG AGCAGCGTGT CAAAAACGCG GATAAGCTAC TAGCTAGAGC CGCGCTAGAG 
CCCAATTTGG TTCCCAAGCC TCCTCGCACA TTTGCCGGCT CTGCTGACCC GTCCTCCGGC 
ATCTCTCTTT CTCCATCCTT CCCGCTCTCA TCTTCCGGAG AACTCAACGA TTTTTCTCCC 
GCTGATGGAG TGTTGCCTGT AAACCATACA TCTACGCTAC GAACTTCCGC TGCTAGTCCC 
AACCCCAAGG AAACTGCATT GTCGTCTATG GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_021477715.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021333390.1 Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa12691
Clone ID Related Accession (Same CDS sequence)	XM_021477715.1 , XM_692781.7
Accession Version	XM_021477715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGCA TGGTACTGTT TCGGCGACGC TGGTCAGTCG GCAGTGATGA CCTGGTGTTG 
CCGGCTTTCT TCCTGTTTGT ACTACACTGC ATATGGCTGG TGGTTCTGTC CGTGGTTCTC 
TTTGGGCTGC CGTACAGTTC AGAGCAGTCA TGTTCAGTCA CGCTGGTGGA TCATGGTCGC 
GGTTACCTCG GCATCTTGGT CAGTTGTCTG ATCTGCGAGA GCGCCATCAT GTGGCTGAGC 
ATGAGGGGCA GCATCCTCTA CACACAACCC AGAGAGGCGG TGCAGTATGT GCTCTATATA 
CGCCTAGCCA TCCTGCTCGT GGAGCTGGTG TATGCAGTGG TGGGCATCGC CTGGTTGGTG 
CAGTACTACC AGCCCTGCCC AGACATCACC GCTAAGAACC TCGCCCTCGG CATTGTGGCA 
TGTAACTGGT TGGTCATCTT CAGTGTGTGT TTCACCATGA TGTGCACCTT TGACCCCACT 
GGACGGACCT TTGTCAAGCT GAAAGCAACT CGTCGACGTC AGCGTAACCT TACAACATAC 
ACACTCAGAC ACAGGCTAGA AGAGGGTCAA GCCAGCAGCT GGAGCAGAAG ACTCAAATTC 
TTCATGTGCT GCACCAGAGC AAAAGACACA CAATCTGACG CTTACTCTGA GGTTGCCAGC 
TTGTTCGCCG AGTTTTTCCG GGATCTGGAT ATCGTGCCGA GTGACATCAT TGCTGGTTTG 
GTGCTGCTCC GACAGAGACA ACGGGCTAAA AGATCAGCCA TCTTGGATCA GGCAAACAAT 
GATGTTTTAG CCTTTCTGTC TGGGATGCCT GTGACACGCA ACACTAAATA CTTGGACCTC 
AAGAACTCGA CTGAGATGGC TATGTATAAG GAAGTGTGCT ACTACATGTT GTTTGCCATG 
GCGGCATATG GCTGGCCCGT TTATCTTTTG AGAAAACCAG CATGTGGACT CTGCAGACTT 
GTCAGCACCT GCTCCTGCAA TACTTCAGTT TCAGGCTCTC GCCTCTCTCA ATCCGTCACC 
GTTGAGGAAG ATAACTGTTG CGGCTGTAAC GTTCTTGCCA TCCGACGCCA GTTTCTAGAC 
CGAGACCTCA AAGAAGTCCA GATTGTCTAC ACATCCTGCC ATGACGCGGT TTATGAGACG 
CCCTTCTTTG TAGCAGTGGA TCACGCAAAG AAGAAAGTCG TGATCAGCAT CAGAGGAACG 
TTGTCTCCTA AGGACGCTTT AACAGATCTC ACTGGCGATT CGGAGCGTTT GCCTGTCGAG 
GAGCAGCACG GCACGTGGCT CGGACACAAG GGAATGGTTT ATTCAGCCGA ATACATCAAG 
AAGAAACTGG AGCAAGAGAT GATCCTCTCT CAAGCCTTTG GGAGAGATTT GAGTAAAGGG 
ACGATGCACT ACGGGCTGGT GATAGTGGGT CATTCTCTGG GAGCGGGAAC AGCCGCCATC 
CTGTCCTTCC TCCTTCGGCC GCAGTATCCT TCACTGCAAT GCTATTCCTA CTCTCCTCCC 
GGCGGCCTGC TTAGTGAGGA TGCAATGGAG TACTCGAAAG AGTTTGTCAC CTCAGTGGTG 
TTGGGAAAAG ATCTGGTGCC CAGGATCGGC CTCTCTCAGC TTGAGGGTTT CCGTCGTCAC 
CTGCTTGAAG TCTTACAGAA GAGCGATAAA CCAAAGTGGA GGATCATTGC TGGAGGAACA 
AAGTGCATCC CTAAATCGGA GCTGCCCATG GATGACGAAG CCCCTGTATC TCAGGGCGTA 
ACACCCTCCA GTTCCCGCCT ATGGCTTCAT CCCAGTGATC TGAGCATCGC CCTGTCAGCC 
TCCACACCGC TGTTTCCCCC GGGACGGGTC ATTCATGTGG TGCACAATCA CCCCCCTGAG 
ATGTGTTGTG GTCAGGAAGA GCCCACGTAC TCTGCTCTGT GGGGCGATAA CAAGGCCTTT 
GATGAGGTCA TCATTTCTCC AGCCATGCTG AATGAACACA TGCCCCATGT GGTGATGGAT 
GGCCTCAACA AGGTGTTGGA AAACTACAAT AAAGGGAAGA CAGCTTTACT GTCTGCTGCT 
AAGATAATGG TCAGTCCAAC AGAAGTGGAC CTGAATCCAG AAACCATCTT CCTGGACACC 
TCAAATTCGC CACAGCCTAC GCCAGCCACA CACCACCGCA GAAACAGCAG TGTCCGTTCC 
TGCGCCCGGT CTGAGATCTC CCTTGATGGC TTCTCTGAGT GTCCGCCTCC TCCTGTCCCT 
ATTGTGCTCA CCGGAGCCCG TGAGCGACTG GCTGTGGAGC TGCGGGAGCG AAAAGCCCCC 
TTGGCCATCA TGGAAAGCCT GTCCGATGCC GAATCCGTCT ACAGCCTGGA CTCTCGCCGA 
TCATCTGCCG CCCTACGGGG CTCACCCTGC CTAGGGAGCC TACCTTTCCC ACTGGATGCC 
CCAATCCCAG AAGAGAATCC TTCATTGAGT TCTCGTACGG AGCTCCTAGC TGCTGAAGGG 
TTGACCCAAG ATTTGGGGGA GGCTGGACCA TACATTCCTA CACCTCCAGA GCCAGAAGAA 
CCCTCCAACG ACATCCCATA CAACAACGAA TACTTCACTC CAGAATTGAA GAGTACCACA 
GATCCAGAAA CAGATTCATC TGTGGTTCCC GACAGTCAAG ACTCCTACCG TGTAGCATCC 
CGGACTGGTT CTCGTCCAGA AAAGCTCAGG GAGTTTGAAG AAAAAGAGGT CAAAGTTGAT 
CTCTCCCAAA CTCTTCCCGC AGTTACTCCA CTATCCAACG GTACTCCCAG TCAGGCGGTC 
CTAGAGTTCG CCCAGTACCT AGACTCTCTG TTTAGGCTGG ACGGAAGCAG TTCGCCTCCT 
CTTGAGCTCT CGGATGCTGA GTCCGAGTCC GGCCGGGGAT CCTACAGTCA GGCTGAGGAA 
CACCAAGAGG AGCAGCGTGT CAAAAACGCG GATAAGCTAC TAGCTAGAGC CGCGCTAGAG 
CCCAATTTGG TTCCCAAGCC TCCTCGCACA TTTGCCGGCT CTGCTGACCC GTCCTCCGGC 
ATCTCTCTTT CTCCATCCTT CCCGCTCTCA TCTTCCGGAG AACTCAACGA TTTTTCTCCC 
GCTGATGGAG TGTTGCCTGT AAACCATACA TCTACGCTAC GAACTTCCGC TGCTAGTCCC 
AACCCCAAGG AAACTGCATT GTCGTCTATG GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333390.1
CDS	737..3892
Translation	MPGMVLFRRRWSVGSDDLVLPAFFLFVLHCIWLVVLSVVLFGLPYSSEQSCSVTLVDHGRGYLGILVSCLICESAIMWLSMRGSILYTQPREAVQYVLYIRLAILLVELVYAVVGIAWLVQYYQPCPDITAKNLALGIVACNWLVIFSVCFTMMCTFDPTGRTFVKLKATRRRQRNLTTYTLRHRLEEGQASSWSRRLKFFMCCTRAKDTQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRSAILDQANNDVLAFLSGMPVTRNTKYLDLKNSTEMAMYKEVCYYMLFAMAAYGWPVYLLRKPACGLCRLVSTCSCNTSVSGSRLSQSVTVEEDNCCGCNVLAIRRQFLDRDLKEVQIVYTSCHDAVYETPFFVAVDHAKKKVVISIRGTLSPKDALTDLTGDSERLPVEEQHGTWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLSKGTMHYGLVIVGHSLGAGTAAILSFLLRPQYPSLQCYSYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLEVLQKSDKPKWRIIAGGTKCIPKSELPMDDEAPVSQGVTPSSSRLWLHPSDLSIALSASTPLFPPGRVIHVVHNHPPEMCCGQEEPTYSALWGDNKAFDEVIISPAMLNEHMPHVVMDGLNKVLENYNKGKTALLSAAKIMVSPTEVDLNPETIFLDTSNSPQPTPATHHRRNSSVRSCARSEISLDGFSECPPPPVPIVLTGARERLAVELRERKAPLAIMESLSDAESVYSLDSRRSSAALRGSPCLGSLPFPLDAPIPEENPSLSSRTELLAAEGLTQDLGEAGPYIPTPPEPEEPSNDIPYNNEYFTPELKSTTDPETDSSVVPDSQDSYRVASRTGSRPEKLREFEEKEVKVDLSQTLPAVTPLSNGTPSQAVLEFAQYLDSLFRLDGSSSPPLELSDAESESGRGSYSQAEEHQEEQRVKNADKLLARAALEPNLVPKPPRTFAGSADPSSGISLSPSFPLSSSGELNDFSPADGVLPVNHTSTLRTSAASPNPKETALSSMV

Target ORF information:

RefSeq Version	XM_021477715.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477715.1

ORF Insert Sequence:

ATGCCGGGCA TGGTACTGTT TCGGCGACGC TGGTCAGTCG GCAGTGATGA CCTGGTGTTG 
CCGGCTTTCT TCCTGTTTGT ACTACACTGC ATATGGCTGG TGGTTCTGTC CGTGGTTCTC 
TTTGGGCTGC CGTACAGTTC AGAGCAGTCA TGTTCAGTCA CGCTGGTGGA TCATGGTCGC 
GGTTACCTCG GCATCTTGGT CAGTTGTCTG ATCTGCGAGA GCGCCATCAT GTGGCTGAGC 
ATGAGGGGCA GCATCCTCTA CACACAACCC AGAGAGGCGG TGCAGTATGT GCTCTATATA 
CGCCTAGCCA TCCTGCTCGT GGAGCTGGTG TATGCAGTGG TGGGCATCGC CTGGTTGGTG 
CAGTACTACC AGCCCTGCCC AGACATCACC GCTAAGAACC TCGCCCTCGG CATTGTGGCA 
TGTAACTGGT TGGTCATCTT CAGTGTGTGT TTCACCATGA TGTGCACCTT TGACCCCACT 
GGACGGACCT TTGTCAAGCT GAAAGCAACT CGTCGACGTC AGCGTAACCT TACAACATAC 
ACACTCAGAC ACAGGCTAGA AGAGGGTCAA GCCAGCAGCT GGAGCAGAAG ACTCAAATTC 
TTCATGTGCT GCACCAGAGC AAAAGACACA CAATCTGACG CTTACTCTGA GGTTGCCAGC 
TTGTTCGCCG AGTTTTTCCG GGATCTGGAT ATCGTGCCGA GTGACATCAT TGCTGGTTTG 
GTGCTGCTCC GACAGAGACA ACGGGCTAAA AGATCAGCCA TCTTGGATCA GGCAAACAAT 
GATGTTTTAG CCTTTCTGTC TGGGATGCCT GTGACACGCA ACACTAAATA CTTGGACCTC 
AAGAACTCGA CTGAGATGGC TATGTATAAG GAAGTGTGCT ACTACATGTT GTTTGCCATG 
GCGGCATATG GCTGGCCCGT TTATCTTTTG AGAAAACCAG CATGTGGACT CTGCAGACTT 
GTCAGCACCT GCTCCTGCAA TACTTCAGTT TCAGGCTCTC GCCTCTCTCA ATCCGTCACC 
GTTGAGGAAG ATAACTGTTG CGGCTGTAAC GTTCTTGCCA TCCGACGCCA GTTTCTAGAC 
CGAGACCTCA AAGAAGTCCA GATTGTCTAC ACATCCTGCC ATGACGCGGT TTATGAGACG 
CCCTTCTTTG TAGCAGTGGA TCACGCAAAG AAGAAAGTCG TGATCAGCAT CAGAGGAACG 
TTGTCTCCTA AGGACGCTTT AACAGATCTC ACTGGCGATT CGGAGCGTTT GCCTGTCGAG 
GAGCAGCACG GCACGTGGCT CGGACACAAG GGAATGGTTT ATTCAGCCGA ATACATCAAG 
AAGAAACTGG AGCAAGAGAT GATCCTCTCT CAAGCCTTTG GGAGAGATTT GAGTAAAGGG 
ACGATGCACT ACGGGCTGGT GATAGTGGGT CATTCTCTGG GAGCGGGAAC AGCCGCCATC 
CTGTCCTTCC TCCTTCGGCC GCAGTATCCT TCACTGCAAT GCTATTCCTA CTCTCCTCCC 
GGCGGCCTGC TTAGTGAGGA TGCAATGGAG TACTCGAAAG AGTTTGTCAC CTCAGTGGTG 
TTGGGAAAAG ATCTGGTGCC CAGGATCGGC CTCTCTCAGC TTGAGGGTTT CCGTCGTCAC 
CTGCTTGAAG TCTTACAGAA GAGCGATAAA CCAAAGTGGA GGATCATTGC TGGAGGAACA 
AAGTGCATCC CTAAATCGGA GCTGCCCATG GATGACGAAG CCCCTGTATC TCAGGGCGTA 
ACACCCTCCA GTTCCCGCCT ATGGCTTCAT CCCAGTGATC TGAGCATCGC CCTGTCAGCC 
TCCACACCGC TGTTTCCCCC GGGACGGGTC ATTCATGTGG TGCACAATCA CCCCCCTGAG 
ATGTGTTGTG GTCAGGAAGA GCCCACGTAC TCTGCTCTGT GGGGCGATAA CAAGGCCTTT 
GATGAGGTCA TCATTTCTCC AGCCATGCTG AATGAACACA TGCCCCATGT GGTGATGGAT 
GGCCTCAACA AGGTGTTGGA AAACTACAAT AAAGGGAAGA CAGCTTTACT GTCTGCTGCT 
AAGATAATGG TCAGTCCAAC AGAAGTGGAC CTGAATCCAG AAACCATCTT CCTGGACACC 
TCAAATTCGC CACAGCCTAC GCCAGCCACA CACCACCGCA GAAACAGCAG TGTCCGTTCC 
TGCGCCCGGT CTGAGATCTC CCTTGATGGC TTCTCTGAGT GTCCGCCTCC TCCTGTCCCT 
ATTGTGCTCA CCGGAGCCCG TGAGCGACTG GCTGTGGAGC TGCGGGAGCG AAAAGCCCCC 
TTGGCCATCA TGGAAAGCCT GTCCGATGCC GAATCCGTCT ACAGCCTGGA CTCTCGCCGA 
TCATCTGCCG CCCTACGGGG CTCACCCTGC CTAGGGAGCC TACCTTTCCC ACTGGATGCC 
CCAATCCCAG AAGAGAATCC TTCATTGAGT TCTCGTACGG AGCTCCTAGC TGCTGAAGGG 
TTGACCCAAG ATTTGGGGGA GGCTGGACCA TACATTCCTA CACCTCCAGA GCCAGAAGAA 
CCCTCCAACG ACATCCCATA CAACAACGAA TACTTCACTC CAGAATTGAA GAGTACCACA 
GATCCAGAAA CAGATTCATC TGTGGTTCCC GACAGTCAAG ACTCCTACCG TGTAGCATCC 
CGGACTGGTT CTCGTCCAGA AAAGCTCAGG GAGTTTGAAG AAAAAGAGGT CAAAGTTGAT 
CTCTCCCAAA CTCTTCCCGC AGTTACTCCA CTATCCAACG GTACTCCCAG TCAGGCGGTC 
CTAGAGTTCG CCCAGTACCT AGACTCTCTG TTTAGGCTGG ACGGAAGCAG TTCGCCTCCT 
CTTGAGCTCT CGGATGCTGA GTCCGAGTCC GGCCGGGGAT CCTACAGTCA GGCTGAGGAA 
CACCAAGAGG AGCAGCGTGT CAAAAACGCG GATAAGCTAC TAGCTAGAGC CGCGCTAGAG 
CCCAATTTGG TTCCCAAGCC TCCTCGCACA TTTGCCGGCT CTGCTGACCC GTCCTCCGGC 
ATCTCTCTTT CTCCATCCTT CCCGCTCTCA TCTTCCGGAG AACTCAACGA TTTTTCTCCC 
GCTGATGGAG TGTTGCCTGT AAACCATACA TCTACGCTAC GAACTTCCGC TGCTAGTCCC 
AACCCCAAGG AAACTGCATT GTCGTCTATG GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_692781.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_697873.5 Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa12691
Clone ID Related Accession (Same CDS sequence)	XM_021477715.1 , XM_692781.7
Accession Version	XM_692781.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_692781.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGCA TGGTACTGTT TCGGCGACGC TGGTCAGTCG GCAGTGATGA CCTGGTGTTG 
CCGGCTTTCT TCCTGTTTGT ACTACACTGC ATATGGCTGG TGGTTCTGTC CGTGGTTCTC 
TTTGGGCTGC CGTACAGTTC AGAGCAGTCA TGTTCAGTCA CGCTGGTGGA TCATGGTCGC 
GGTTACCTCG GCATCTTGGT CAGTTGTCTG ATCTGCGAGA GCGCCATCAT GTGGCTGAGC 
ATGAGGGGCA GCATCCTCTA CACACAACCC AGAGAGGCGG TGCAGTATGT GCTCTATATA 
CGCCTAGCCA TCCTGCTCGT GGAGCTGGTG TATGCAGTGG TGGGCATCGC CTGGTTGGTG 
CAGTACTACC AGCCCTGCCC AGACATCACC GCTAAGAACC TCGCCCTCGG CATTGTGGCA 
TGTAACTGGT TGGTCATCTT CAGTGTGTGT TTCACCATGA TGTGCACCTT TGACCCCACT 
GGACGGACCT TTGTCAAGCT GAAAGCAACT CGTCGACGTC AGCGTAACCT TACAACATAC 
ACACTCAGAC ACAGGCTAGA AGAGGGTCAA GCCAGCAGCT GGAGCAGAAG ACTCAAATTC 
TTCATGTGCT GCACCAGAGC AAAAGACACA CAATCTGACG CTTACTCTGA GGTTGCCAGC 
TTGTTCGCCG AGTTTTTCCG GGATCTGGAT ATCGTGCCGA GTGACATCAT TGCTGGTTTG 
GTGCTGCTCC GACAGAGACA ACGGGCTAAA AGATCAGCCA TCTTGGATCA GGCAAACAAT 
GATGTTTTAG CCTTTCTGTC TGGGATGCCT GTGACACGCA ACACTAAATA CTTGGACCTC 
AAGAACTCGA CTGAGATGGC TATGTATAAG GAAGTGTGCT ACTACATGTT GTTTGCCATG 
GCGGCATATG GCTGGCCCGT TTATCTTTTG AGAAAACCAG CATGTGGACT CTGCAGACTT 
GTCAGCACCT GCTCCTGCAA TACTTCAGTT TCAGGCTCTC GCCTCTCTCA ATCCGTCACC 
GTTGAGGAAG ATAACTGTTG CGGCTGTAAC GTTCTTGCCA TCCGACGCCA GTTTCTAGAC 
CGAGACCTCA AAGAAGTCCA GATTGTCTAC ACATCCTGCC ATGACGCGGT TTATGAGACG 
CCCTTCTTTG TAGCAGTGGA TCACGCAAAG AAGAAAGTCG TGATCAGCAT CAGAGGAACG 
TTGTCTCCTA AGGACGCTTT AACAGATCTC ACTGGCGATT CGGAGCGTTT GCCTGTCGAG 
GAGCAGCACG GCACGTGGCT CGGACACAAG GGAATGGTTT ATTCAGCCGA ATACATCAAG 
AAGAAACTGG AGCAAGAGAT GATCCTCTCT CAAGCCTTTG GGAGAGATTT GAGTAAAGGG 
ACGATGCACT ACGGGCTGGT GATAGTGGGT CATTCTCTGG GAGCGGGAAC AGCCGCCATC 
CTGTCCTTCC TCCTTCGGCC GCAGTATCCT TCACTGCAAT GCTATTCCTA CTCTCCTCCC 
GGCGGCCTGC TTAGTGAGGA TGCAATGGAG TACTCGAAAG AGTTTGTCAC CTCAGTGGTG 
TTGGGAAAAG ATCTGGTGCC CAGGATCGGC CTCTCTCAGC TTGAGGGTTT CCGTCGTCAC 
CTGCTTGAAG TCTTACAGAA GAGCGATAAA CCAAAGTGGA GGATCATTGC TGGAGGAACA 
AAGTGCATCC CTAAATCGGA GCTGCCCATG GATGACGAAG CCCCTGTATC TCAGGGCGTA 
ACACCCTCCA GTTCCCGCCT ATGGCTTCAT CCCAGTGATC TGAGCATCGC CCTGTCAGCC 
TCCACACCGC TGTTTCCCCC GGGACGGGTC ATTCATGTGG TGCACAATCA CCCCCCTGAG 
ATGTGTTGTG GTCAGGAAGA GCCCACGTAC TCTGCTCTGT GGGGCGATAA CAAGGCCTTT 
GATGAGGTCA TCATTTCTCC AGCCATGCTG AATGAACACA TGCCCCATGT GGTGATGGAT 
GGCCTCAACA AGGTGTTGGA AAACTACAAT AAAGGGAAGA CAGCTTTACT GTCTGCTGCT 
AAGATAATGG TCAGTCCAAC AGAAGTGGAC CTGAATCCAG AAACCATCTT CCTGGACACC 
TCAAATTCGC CACAGCCTAC GCCAGCCACA CACCACCGCA GAAACAGCAG TGTCCGTTCC 
TGCGCCCGGT CTGAGATCTC CCTTGATGGC TTCTCTGAGT GTCCGCCTCC TCCTGTCCCT 
ATTGTGCTCA CCGGAGCCCG TGAGCGACTG GCTGTGGAGC TGCGGGAGCG AAAAGCCCCC 
TTGGCCATCA TGGAAAGCCT GTCCGATGCC GAATCCGTCT ACAGCCTGGA CTCTCGCCGA 
TCATCTGCCG CCCTACGGGG CTCACCCTGC CTAGGGAGCC TACCTTTCCC ACTGGATGCC 
CCAATCCCAG AAGAGAATCC TTCATTGAGT TCTCGTACGG AGCTCCTAGC TGCTGAAGGG 
TTGACCCAAG ATTTGGGGGA GGCTGGACCA TACATTCCTA CACCTCCAGA GCCAGAAGAA 
CCCTCCAACG ACATCCCATA CAACAACGAA TACTTCACTC CAGAATTGAA GAGTACCACA 
GATCCAGAAA CAGATTCATC TGTGGTTCCC GACAGTCAAG ACTCCTACCG TGTAGCATCC 
CGGACTGGTT CTCGTCCAGA AAAGCTCAGG GAGTTTGAAG AAAAAGAGGT CAAAGTTGAT 
CTCTCCCAAA CTCTTCCCGC AGTTACTCCA CTATCCAACG GTACTCCCAG TCAGGCGGTC 
CTAGAGTTCG CCCAGTACCT AGACTCTCTG TTTAGGCTGG ACGGAAGCAG TTCGCCTCCT 
CTTGAGCTCT CGGATGCTGA GTCCGAGTCC GGCCGGGGAT CCTACAGTCA GGCTGAGGAA 
CACCAAGAGG AGCAGCGTGT CAAAAACGCG GATAAGCTAC TAGCTAGAGC CGCGCTAGAG 
CCCAATTTGG TTCCCAAGCC TCCTCGCACA TTTGCCGGCT CTGCTGACCC GTCCTCCGGC 
ATCTCTCTTT CTCCATCCTT CCCGCTCTCA TCTTCCGGAG AACTCAACGA TTTTTCTCCC 
GCTGATGGAG TGTTGCCTGT AAACCATACA TCTACGCTAC GAACTTCCGC TGCTAGTCCC 
AACCCCAAGG AAACTGCATT GTCGTCTATG GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_697873.5
CDS	741..3896
Translation	MPGMVLFRRRWSVGSDDLVLPAFFLFVLHCIWLVVLSVVLFGLPYSSEQSCSVTLVDHGRGYLGILVSCLICESAIMWLSMRGSILYTQPREAVQYVLYIRLAILLVELVYAVVGIAWLVQYYQPCPDITAKNLALGIVACNWLVIFSVCFTMMCTFDPTGRTFVKLKATRRRQRNLTTYTLRHRLEEGQASSWSRRLKFFMCCTRAKDTQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRSAILDQANNDVLAFLSGMPVTRNTKYLDLKNSTEMAMYKEVCYYMLFAMAAYGWPVYLLRKPACGLCRLVSTCSCNTSVSGSRLSQSVTVEEDNCCGCNVLAIRRQFLDRDLKEVQIVYTSCHDAVYETPFFVAVDHAKKKVVISIRGTLSPKDALTDLTGDSERLPVEEQHGTWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLSKGTMHYGLVIVGHSLGAGTAAILSFLLRPQYPSLQCYSYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLEVLQKSDKPKWRIIAGGTKCIPKSELPMDDEAPVSQGVTPSSSRLWLHPSDLSIALSASTPLFPPGRVIHVVHNHPPEMCCGQEEPTYSALWGDNKAFDEVIISPAMLNEHMPHVVMDGLNKVLENYNKGKTALLSAAKIMVSPTEVDLNPETIFLDTSNSPQPTPATHHRRNSSVRSCARSEISLDGFSECPPPPVPIVLTGARERLAVELRERKAPLAIMESLSDAESVYSLDSRRSSAALRGSPCLGSLPFPLDAPIPEENPSLSSRTELLAAEGLTQDLGEAGPYIPTPPEPEEPSNDIPYNNEYFTPELKSTTDPETDSSVVPDSQDSYRVASRTGSRPEKLREFEEKEVKVDLSQTLPAVTPLSNGTPSQAVLEFAQYLDSLFRLDGSSSPPLELSDAESESGRGSYSQAEEHQEEQRVKNADKLLARAALEPNLVPKPPRTFAGSADPSSGISLSPSFPLSSSGELNDFSPADGVLPVNHTSTLRTSAASPNPKETALSSMV

Target ORF information:

RefSeq Version	XM_692781.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol lipase, alpha (dagla), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_692781.7

ORF Insert Sequence:

ATGCCGGGCA TGGTACTGTT TCGGCGACGC TGGTCAGTCG GCAGTGATGA CCTGGTGTTG 
CCGGCTTTCT TCCTGTTTGT ACTACACTGC ATATGGCTGG TGGTTCTGTC CGTGGTTCTC 
TTTGGGCTGC CGTACAGTTC AGAGCAGTCA TGTTCAGTCA CGCTGGTGGA TCATGGTCGC 
GGTTACCTCG GCATCTTGGT CAGTTGTCTG ATCTGCGAGA GCGCCATCAT GTGGCTGAGC 
ATGAGGGGCA GCATCCTCTA CACACAACCC AGAGAGGCGG TGCAGTATGT GCTCTATATA 
CGCCTAGCCA TCCTGCTCGT GGAGCTGGTG TATGCAGTGG TGGGCATCGC CTGGTTGGTG 
CAGTACTACC AGCCCTGCCC AGACATCACC GCTAAGAACC TCGCCCTCGG CATTGTGGCA 
TGTAACTGGT TGGTCATCTT CAGTGTGTGT TTCACCATGA TGTGCACCTT TGACCCCACT 
GGACGGACCT TTGTCAAGCT GAAAGCAACT CGTCGACGTC AGCGTAACCT TACAACATAC 
ACACTCAGAC ACAGGCTAGA AGAGGGTCAA GCCAGCAGCT GGAGCAGAAG ACTCAAATTC 
TTCATGTGCT GCACCAGAGC AAAAGACACA CAATCTGACG CTTACTCTGA GGTTGCCAGC 
TTGTTCGCCG AGTTTTTCCG GGATCTGGAT ATCGTGCCGA GTGACATCAT TGCTGGTTTG 
GTGCTGCTCC GACAGAGACA ACGGGCTAAA AGATCAGCCA TCTTGGATCA GGCAAACAAT 
GATGTTTTAG CCTTTCTGTC TGGGATGCCT GTGACACGCA ACACTAAATA CTTGGACCTC 
AAGAACTCGA CTGAGATGGC TATGTATAAG GAAGTGTGCT ACTACATGTT GTTTGCCATG 
GCGGCATATG GCTGGCCCGT TTATCTTTTG AGAAAACCAG CATGTGGACT CTGCAGACTT 
GTCAGCACCT GCTCCTGCAA TACTTCAGTT TCAGGCTCTC GCCTCTCTCA ATCCGTCACC 
GTTGAGGAAG ATAACTGTTG CGGCTGTAAC GTTCTTGCCA TCCGACGCCA GTTTCTAGAC 
CGAGACCTCA AAGAAGTCCA GATTGTCTAC ACATCCTGCC ATGACGCGGT TTATGAGACG 
CCCTTCTTTG TAGCAGTGGA TCACGCAAAG AAGAAAGTCG TGATCAGCAT CAGAGGAACG 
TTGTCTCCTA AGGACGCTTT AACAGATCTC ACTGGCGATT CGGAGCGTTT GCCTGTCGAG 
GAGCAGCACG GCACGTGGCT CGGACACAAG GGAATGGTTT ATTCAGCCGA ATACATCAAG 
AAGAAACTGG AGCAAGAGAT GATCCTCTCT CAAGCCTTTG GGAGAGATTT GAGTAAAGGG 
ACGATGCACT ACGGGCTGGT GATAGTGGGT CATTCTCTGG GAGCGGGAAC AGCCGCCATC 
CTGTCCTTCC TCCTTCGGCC GCAGTATCCT TCACTGCAAT GCTATTCCTA CTCTCCTCCC 
GGCGGCCTGC TTAGTGAGGA TGCAATGGAG TACTCGAAAG AGTTTGTCAC CTCAGTGGTG 
TTGGGAAAAG ATCTGGTGCC CAGGATCGGC CTCTCTCAGC TTGAGGGTTT CCGTCGTCAC 
CTGCTTGAAG TCTTACAGAA GAGCGATAAA CCAAAGTGGA GGATCATTGC TGGAGGAACA 
AAGTGCATCC CTAAATCGGA GCTGCCCATG GATGACGAAG CCCCTGTATC TCAGGGCGTA 
ACACCCTCCA GTTCCCGCCT ATGGCTTCAT CCCAGTGATC TGAGCATCGC CCTGTCAGCC 
TCCACACCGC TGTTTCCCCC GGGACGGGTC ATTCATGTGG TGCACAATCA CCCCCCTGAG 
ATGTGTTGTG GTCAGGAAGA GCCCACGTAC TCTGCTCTGT GGGGCGATAA CAAGGCCTTT 
GATGAGGTCA TCATTTCTCC AGCCATGCTG AATGAACACA TGCCCCATGT GGTGATGGAT 
GGCCTCAACA AGGTGTTGGA AAACTACAAT AAAGGGAAGA CAGCTTTACT GTCTGCTGCT 
AAGATAATGG TCAGTCCAAC AGAAGTGGAC CTGAATCCAG AAACCATCTT CCTGGACACC 
TCAAATTCGC CACAGCCTAC GCCAGCCACA CACCACCGCA GAAACAGCAG TGTCCGTTCC 
TGCGCCCGGT CTGAGATCTC CCTTGATGGC TTCTCTGAGT GTCCGCCTCC TCCTGTCCCT 
ATTGTGCTCA CCGGAGCCCG TGAGCGACTG GCTGTGGAGC TGCGGGAGCG AAAAGCCCCC 
TTGGCCATCA TGGAAAGCCT GTCCGATGCC GAATCCGTCT ACAGCCTGGA CTCTCGCCGA 
TCATCTGCCG CCCTACGGGG CTCACCCTGC CTAGGGAGCC TACCTTTCCC ACTGGATGCC 
CCAATCCCAG AAGAGAATCC TTCATTGAGT TCTCGTACGG AGCTCCTAGC TGCTGAAGGG 
TTGACCCAAG ATTTGGGGGA GGCTGGACCA TACATTCCTA CACCTCCAGA GCCAGAAGAA 
CCCTCCAACG ACATCCCATA CAACAACGAA TACTTCACTC CAGAATTGAA GAGTACCACA 
GATCCAGAAA CAGATTCATC TGTGGTTCCC GACAGTCAAG ACTCCTACCG TGTAGCATCC 
CGGACTGGTT CTCGTCCAGA AAAGCTCAGG GAGTTTGAAG AAAAAGAGGT CAAAGTTGAT 
CTCTCCCAAA CTCTTCCCGC AGTTACTCCA CTATCCAACG GTACTCCCAG TCAGGCGGTC 
CTAGAGTTCG CCCAGTACCT AGACTCTCTG TTTAGGCTGG ACGGAAGCAG TTCGCCTCCT 
CTTGAGCTCT CGGATGCTGA GTCCGAGTCC GGCCGGGGAT CCTACAGTCA GGCTGAGGAA 
CACCAAGAGG AGCAGCGTGT CAAAAACGCG GATAAGCTAC TAGCTAGAGC CGCGCTAGAG 
CCCAATTTGG TTCCCAAGCC TCCTCGCACA TTTGCCGGCT CTGCTGACCC GTCCTCCGGC 
ATCTCTCTTT CTCCATCCTT CCCGCTCTCA TCTTCCGGAG AACTCAACGA TTTTTCTCCC 
GCTGATGGAG TGTTGCCTGT AAACCATACA TCTACGCTAC GAACTTCCGC TGCTAGTCCC 
AACCCCAAGG AAACTGCATT GTCGTCTATG GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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DAGLA cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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