dagla cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dagla
Entrez Gene ID	100498478
Full Name	diacylglycerol lipase, alpha
General protein information	Preferred Names diacylglycerol lipase, alpha Names sn1-specific diacylglycerol lipase alpha
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004913409.2	XP_004913466.1	sn1-specific diacylglycerol lipase alpha isoform X1
XM_031900543.1	XP_031756403.1	sn1-specific diacylglycerol lipase alpha
XM_004913409.3	XP_004913466.1	sn1-specific diacylglycerol lipase alpha
XM_004913408.1	XP_004913465.1	sn1-specific diacylglycerol lipase alpha isoform X1
XM_004913410.1	XP_004913467.1	sn1-specific diacylglycerol lipase alpha isoform X2
XM_018093320.1	XP_017948809.1	sn1-specific diacylglycerol lipase alpha isoform X2
XM_004913408.2	XP_004913465.1	sn1-specific diacylglycerol lipase alpha
XM_004913411.1	XP_004913468.1	sn1-specific diacylglycerol lipase alpha isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	dagla	XP_697873.5
Homo sapiens (human)	DAGLA	NP_006124.1
Canis lupus familiaris (dog)	DAGLA	XP_005631813.1
Mus musculus (house mouse)	Dagla	NP_932782.2
Gallus gallus (chicken)	DAGLA	XP_423696.3
Xenopus tropicalis (tropical clawed frog)	dagla	XP_004913466.1
Rattus norvegicus (Norway rat)	Dagla	NP_001005886.1
Pan troglodytes (chimpanzee)	DAGLA	XP_508479.4
Bos taurus (cattle)	DAGLA	NP_001179512.1

Gene Ontology

The following dagla gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dagla cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa08579	XM_004913410.1 Latest version!	Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa08579	XM_018093320.1 Latest version!	Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa08580	XM_004913411.1 Latest version!	Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa56567	XM_031900543.1 Latest version!	Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa56567	XM_004913408.2 Latest version!	Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa56567	XM_004913409.3 Latest version!	Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa08578	XM_004913408.1 Latest version!	Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa08578	XM_004913409.2 Latest version!	Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dagla ( XM_004913410.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913467.1 Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa08579
Clone ID Related Accession (Same CDS sequence)	XM_004913410.1 , XM_018093320.1
Accession Version	XM_004913410.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sn1-specific diacylglycerol lipase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCAG AAATGGCCAT TATCTGGCTG AGCATGAGAG GGGGCATCCT ATACACCGAG 
CCTCGGGACT CCATGCAGTA TGTGCTCTAC GTGCGCCTGG CGATCCTGGT GATTGAGTTC 
ATATACGCAA TCGTGGGCAT TGTCTGGCTG GCTCAGTATT ACACGTCGTG CAATGATGTC 
ACTGCCAAAA ATGTCACACT GGGGATGGTG GTGTGTAACT GGGTGGTCAT ACTCAGTGTG 
TGCATCACAG TCCTCTGTGT GTTCGATCCA ACCGGAAGAA CTTTCGTGAA GCTGAGGGCA 
ACAAAAAGGA GACAGAGAAA CCTGCGTACA TACAACCTGC GGCACCGACT AGAAGAAGGC 
CAGGCCAGCA GCTGGACACG GCGCCTGAAG GTGTTTCTGT GCTGCACGCG CACTAAAGAC 
TCCCAGTCTG ATGCATACTC TGAGATCGCC TACTTATTTG CTGAGTTTTT CAGAGATCTT 
GACATTGTTC CATCAGACAT CATTGCTGGA CTGGTTCTAC TGCGCCAGCG TCAAAGGGCA 
AAACGCAACG CTGTTCTCGA TGAGGCAAAC AATGACATAT TGGCTTTTCT ATCTGGGATG 
CCCGTCACCA GGAAAACAAA ATACCTAGAC CTGAAAAATG CACAAGAGAT GCAGCGGTAC 
AAGGAGGTCT GTTACTACAT GCTCTTTGCG CTGGCAGCAT ATGGCTGGCC TATGTACCTC 
ATTAGGAACC CAACCTGTGG CATCTGCCGT TTGGCAGGGG CATGCTCCAG TTGCTGCTGC 
CTTTGCTCAG CAAGGCCGCG CTTTGCCCCC GGGGTCACAA TTGAGGAGGA TAACTGCTGC 
GGCTGTAACG CCATCGCCAT AAAACGCCAC TTCTTAGACG AGAACATGAA CATGGTGGAC 
ATTGTGTATA CTTCATGCCA TGATGCTGTT TATGAAACAC CATTCTTTGT TGCAGTTGAT 
CATGATAAGA AGAAAGTGGT GATTAGCATT CGAGGGACAC TCTCTCCCAA GGATGCCTTG 
ACAGATCTGA CAGGAGATGC GGAACGGTTG CCAGTGGAAG GGCATCATGG CACCTGGTTG 
GGACACAAGG GAATGGTTCT GTCAGCTGAA TACATTAAAA AGAAGCTGGA GCAAGAGATG 
GTTCTTTCCC AAGCATTTGG CCGTGACCTG GCACGTGGGA CCAAACACTA TGAACTGATT 
GTGGTTGGCC ATTCGCTTGG AGCTGGAACA GCTGCCATCT TGTCCTTCCT GTTACGCCCG 
CAGTATCCTT CCCTGAAGTG CTTTGCATAT TCCCCACCTG GTGGCTTGTT AAGTGAGGAT 
GCGATGGAAT ATTCTAAGGA ATTTGTTACC TCTGTGGTGC TTGGAAAGGA TCTAGTTCCA 
AGAATCGGCC TCTCGCAGCT GGAGGGTTTT CGCAGGCACT TGCTTGATGT TCTTCAGAGA 
AGCAACAAGC CTAAGTGGCG CATCATACTT GGCGGAACCA AATGCATACC TAAATCAGAA 
CTCCCGGAAG AGTCAGAGGC TATAACGATA CCCAGTAATC GCCTATGGAC GCACCCCAGT 
GACCTGACCA TAGCACTTTC AGCTAGCACA CCCCTTTACC CACCGGGGCG TATCATTCAT 
GTGGTTCACA ACCATCCAGT TGAGCAATGC TGTTTCTGGA ATCAGGAAGA ACCCACCTAC 
TTTGCCATCT GGGGTGATAA TAAGGCATTT GAGGAGGTCA TAATCTCTCC AGCCATGTTG 
CATGAACATC TCCCACACGT GGTAATGGAA GGACTGAACA AGGTCCTAGA GAATTACAAC 
AAGGGGAAAA CCGCGCTGCT CTCCGCTGCC AAGGTCATGG TGAGTCCAAC GGAAGTGGAT 
CTGAACCCAG AGCTGATTTT CCAACAGCAA CCTCACCCGC CTCCCCCAAT CCTGGAGGTA 
ATTCCTGCCA TCATGCCTGG AGCCCACAAC AGGAGGAACA GCAGCACCAA GAGTAAGCAG 
TCAGACATCA GCCTGGAAGG TTTCTATGAC TCTCGCCTTC TGTCACCAAT TCTAAAAGAC 
CCAGTAGAGC TCCTTTTGAT GAGCACAAAA GATTGCCTGG CTGTATCTCT CCAAGATCGC 
AGGGCACCTC TCGCCACCAT GGAGAGCCTC TCAGATAATG AATCTGTTTA CAGCTTTGAT 
TCACGGCGCT CTTCTGGTTT CCGCAGTATA CGAGGCTCAC CGAGCCTGAG AGCAGTGTTG 
GAGCGCGATG AGAGTCATTG TTTCTTCATT GACCCCACGA TCCCTGAGGA AAACCCCTCT 
TTGAGCTCAC GGACAGAGCT TCTAGGAGCA GATGCGCTCT CAAAGCATTC GCAGGAGAGC 
CAGCCGACTG AGGTTGCTCA CAATAGTGGC AGATCTTCAC CAGGTGACCT GCCTCCTTGG 
GATGAGGAGG CCTTAGCAGG AGTAGAGAGC AGCAGGGCTC TCACACCAGC TAAAGAAGAA 
TTGGCACTGC AGAATGGGCG CTTGAATGAG GTACCTAGTC CCCAGGTGCT GGAGTTTGCA 
GAATTTATAG ACAGCCTTTT TAATCTGGAC AACAAAAGTG GTTCTCTGCA GGATCTTTAC 
CATGTGATGG TTTCAGATGG TACAGACCGT GATATACCTA AGTCAGTTAG TGACCAGGAA 
ATCCTACTTC GGGCAAAGTT TGAACCTAAT CTGGTGCCCA AGCCCCCTCG CCTCTTTGCT 
GGCTCTGCAG ATCCATCCTC TGGCATTTCA GTGTCCGCCT CCTTCCCTCT CAGCTCCTCT 
GGGGAGCTCA TGGACTTAAC GCCAACGGGT ATGAGCAGCC AGGAGTGCCT GACGCCAGAC 
CCCATCCGAA CTCCAACGCC AACAGGAGCC AGTAACGGCA AAAAGGAAGA GTTGCTTATT 
TCTGCTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913467.1
CDS	1150..4041
Translation	MIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLAQYYTSCNDVTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQASSWTRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRKTKYLDLKNAQEMQRYKEVCYYMLFALAAYGWPMYLIRNPTCGICRLAGACSSCCCLCSARPRFAPGVTIEEDNCCGCNAIAIKRHFLDENMNMVDIVYTSCHDAVYETPFFVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLARGTKHYELIVVGHSLGAGTAAILSFLLRPQYPSLKCFAYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLDVLQRSNKPKWRIILGGTKCIPKSELPEESEAITIPSNRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPVEQCCFWNQEEPTYFAIWGDNKAFEEVIISPAMLHEHLPHVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLNPELIFQQQPHPPPPILEVIPAIMPGAHNRRNSSTKSKQSDISLEGFYDSRLLSPILKDPVELLLMSTKDCLAVSLQDRRAPLATMESLSDNESVYSFDSRRSSGFRSIRGSPSLRAVLERDESHCFFIDPTIPEENPSLSSRTELLGADALSKHSQESQPTEVAHNSGRSSPGDLPPWDEEALAGVESSRALTPAKEELALQNGRLNEVPSPQVLEFAEFIDSLFNLDNKSGSLQDLYHVMVSDGTDRDIPKSVSDQEILLRAKFEPNLVPKPPRLFAGSADPSSGISVSASFPLSSSGELMDLTPTGMSSQECLTPDPIRTPTPTGASNGKKEELLISAL

Target ORF information:

RefSeq Version	XM_004913410.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913410.1

ORF Insert Sequence:

ATGATTGCAG AAATGGCCAT TATCTGGCTG AGCATGAGAG GGGGCATCCT ATACACCGAG 
CCTCGGGACT CCATGCAGTA TGTGCTCTAC GTGCGCCTGG CGATCCTGGT GATTGAGTTC 
ATATACGCAA TCGTGGGCAT TGTCTGGCTG GCTCAGTATT ACACGTCGTG CAATGATGTC 
ACTGCCAAAA ATGTCACACT GGGGATGGTG GTGTGTAACT GGGTGGTCAT ACTCAGTGTG 
TGCATCACAG TCCTCTGTGT GTTCGATCCA ACCGGAAGAA CTTTCGTGAA GCTGAGGGCA 
ACAAAAAGGA GACAGAGAAA CCTGCGTACA TACAACCTGC GGCACCGACT AGAAGAAGGC 
CAGGCCAGCA GCTGGACACG GCGCCTGAAG GTGTTTCTGT GCTGCACGCG CACTAAAGAC 
TCCCAGTCTG ATGCATACTC TGAGATCGCC TACTTATTTG CTGAGTTTTT CAGAGATCTT 
GACATTGTTC CATCAGACAT CATTGCTGGA CTGGTTCTAC TGCGCCAGCG TCAAAGGGCA 
AAACGCAACG CTGTTCTCGA TGAGGCAAAC AATGACATAT TGGCTTTTCT ATCTGGGATG 
CCCGTCACCA GGAAAACAAA ATACCTAGAC CTGAAAAATG CACAAGAGAT GCAGCGGTAC 
AAGGAGGTCT GTTACTACAT GCTCTTTGCG CTGGCAGCAT ATGGCTGGCC TATGTACCTC 
ATTAGGAACC CAACCTGTGG CATCTGCCGT TTGGCAGGGG CATGCTCCAG TTGCTGCTGC 
CTTTGCTCAG CAAGGCCGCG CTTTGCCCCC GGGGTCACAA TTGAGGAGGA TAACTGCTGC 
GGCTGTAACG CCATCGCCAT AAAACGCCAC TTCTTAGACG AGAACATGAA CATGGTGGAC 
ATTGTGTATA CTTCATGCCA TGATGCTGTT TATGAAACAC CATTCTTTGT TGCAGTTGAT 
CATGATAAGA AGAAAGTGGT GATTAGCATT CGAGGGACAC TCTCTCCCAA GGATGCCTTG 
ACAGATCTGA CAGGAGATGC GGAACGGTTG CCAGTGGAAG GGCATCATGG CACCTGGTTG 
GGACACAAGG GAATGGTTCT GTCAGCTGAA TACATTAAAA AGAAGCTGGA GCAAGAGATG 
GTTCTTTCCC AAGCATTTGG CCGTGACCTG GCACGTGGGA CCAAACACTA TGAACTGATT 
GTGGTTGGCC ATTCGCTTGG AGCTGGAACA GCTGCCATCT TGTCCTTCCT GTTACGCCCG 
CAGTATCCTT CCCTGAAGTG CTTTGCATAT TCCCCACCTG GTGGCTTGTT AAGTGAGGAT 
GCGATGGAAT ATTCTAAGGA ATTTGTTACC TCTGTGGTGC TTGGAAAGGA TCTAGTTCCA 
AGAATCGGCC TCTCGCAGCT GGAGGGTTTT CGCAGGCACT TGCTTGATGT TCTTCAGAGA 
AGCAACAAGC CTAAGTGGCG CATCATACTT GGCGGAACCA AATGCATACC TAAATCAGAA 
CTCCCGGAAG AGTCAGAGGC TATAACGATA CCCAGTAATC GCCTATGGAC GCACCCCAGT 
GACCTGACCA TAGCACTTTC AGCTAGCACA CCCCTTTACC CACCGGGGCG TATCATTCAT 
GTGGTTCACA ACCATCCAGT TGAGCAATGC TGTTTCTGGA ATCAGGAAGA ACCCACCTAC 
TTTGCCATCT GGGGTGATAA TAAGGCATTT GAGGAGGTCA TAATCTCTCC AGCCATGTTG 
CATGAACATC TCCCACACGT GGTAATGGAA GGACTGAACA AGGTCCTAGA GAATTACAAC 
AAGGGGAAAA CCGCGCTGCT CTCCGCTGCC AAGGTCATGG TGAGTCCAAC GGAAGTGGAT 
CTGAACCCAG AGCTGATTTT CCAACAGCAA CCTCACCCGC CTCCCCCAAT CCTGGAGGTA 
ATTCCTGCCA TCATGCCTGG AGCCCACAAC AGGAGGAACA GCAGCACCAA GAGTAAGCAG 
TCAGACATCA GCCTGGAAGG TTTCTATGAC TCTCGCCTTC TGTCACCAAT TCTAAAAGAC 
CCAGTAGAGC TCCTTTTGAT GAGCACAAAA GATTGCCTGG CTGTATCTCT CCAAGATCGC 
AGGGCACCTC TCGCCACCAT GGAGAGCCTC TCAGATAATG AATCTGTTTA CAGCTTTGAT 
TCACGGCGCT CTTCTGGTTT CCGCAGTATA CGAGGCTCAC CGAGCCTGAG AGCAGTGTTG 
GAGCGCGATG AGAGTCATTG TTTCTTCATT GACCCCACGA TCCCTGAGGA AAACCCCTCT 
TTGAGCTCAC GGACAGAGCT TCTAGGAGCA GATGCGCTCT CAAAGCATTC GCAGGAGAGC 
CAGCCGACTG AGGTTGCTCA CAATAGTGGC AGATCTTCAC CAGGTGACCT GCCTCCTTGG 
GATGAGGAGG CCTTAGCAGG AGTAGAGAGC AGCAGGGCTC TCACACCAGC TAAAGAAGAA 
TTGGCACTGC AGAATGGGCG CTTGAATGAG GTACCTAGTC CCCAGGTGCT GGAGTTTGCA 
GAATTTATAG ACAGCCTTTT TAATCTGGAC AACAAAAGTG GTTCTCTGCA GGATCTTTAC 
CATGTGATGG TTTCAGATGG TACAGACCGT GATATACCTA AGTCAGTTAG TGACCAGGAA 
ATCCTACTTC GGGCAAAGTT TGAACCTAAT CTGGTGCCCA AGCCCCCTCG CCTCTTTGCT 
GGCTCTGCAG ATCCATCCTC TGGCATTTCA GTGTCCGCCT CCTTCCCTCT CAGCTCCTCT 
GGGGAGCTCA TGGACTTAAC GCCAACGGGT ATGAGCAGCC AGGAGTGCCT GACGCCAGAC 
CCCATCCGAA CTCCAACGCC AACAGGAGCC AGTAACGGCA AAAAGGAAGA GTTGCTTATT 
TCTGCTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dagla ( XM_018093320.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948809.1 Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa08579
Clone ID Related Accession (Same CDS sequence)	XM_004913410.1 , XM_018093320.1
Accession Version	XM_018093320.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sn1-specific diacylglycerol lipase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCAG AAATGGCCAT TATCTGGCTG AGCATGAGAG GGGGCATCCT ATACACCGAG 
CCTCGGGACT CCATGCAGTA TGTGCTCTAC GTGCGCCTGG CGATCCTGGT GATTGAGTTC 
ATATACGCAA TCGTGGGCAT TGTCTGGCTG GCTCAGTATT ACACGTCGTG CAATGATGTC 
ACTGCCAAAA ATGTCACACT GGGGATGGTG GTGTGTAACT GGGTGGTCAT ACTCAGTGTG 
TGCATCACAG TCCTCTGTGT GTTCGATCCA ACCGGAAGAA CTTTCGTGAA GCTGAGGGCA 
ACAAAAAGGA GACAGAGAAA CCTGCGTACA TACAACCTGC GGCACCGACT AGAAGAAGGC 
CAGGCCAGCA GCTGGACACG GCGCCTGAAG GTGTTTCTGT GCTGCACGCG CACTAAAGAC 
TCCCAGTCTG ATGCATACTC TGAGATCGCC TACTTATTTG CTGAGTTTTT CAGAGATCTT 
GACATTGTTC CATCAGACAT CATTGCTGGA CTGGTTCTAC TGCGCCAGCG TCAAAGGGCA 
AAACGCAACG CTGTTCTCGA TGAGGCAAAC AATGACATAT TGGCTTTTCT ATCTGGGATG 
CCCGTCACCA GGAAAACAAA ATACCTAGAC CTGAAAAATG CACAAGAGAT GCAGCGGTAC 
AAGGAGGTCT GTTACTACAT GCTCTTTGCG CTGGCAGCAT ATGGCTGGCC TATGTACCTC 
ATTAGGAACC CAACCTGTGG CATCTGCCGT TTGGCAGGGG CATGCTCCAG TTGCTGCTGC 
CTTTGCTCAG CAAGGCCGCG CTTTGCCCCC GGGGTCACAA TTGAGGAGGA TAACTGCTGC 
GGCTGTAACG CCATCGCCAT AAAACGCCAC TTCTTAGACG AGAACATGAA CATGGTGGAC 
ATTGTGTATA CTTCATGCCA TGATGCTGTT TATGAAACAC CATTCTTTGT TGCAGTTGAT 
CATGATAAGA AGAAAGTGGT GATTAGCATT CGAGGGACAC TCTCTCCCAA GGATGCCTTG 
ACAGATCTGA CAGGAGATGC GGAACGGTTG CCAGTGGAAG GGCATCATGG CACCTGGTTG 
GGACACAAGG GAATGGTTCT GTCAGCTGAA TACATTAAAA AGAAGCTGGA GCAAGAGATG 
GTTCTTTCCC AAGCATTTGG CCGTGACCTG GCACGTGGGA CCAAACACTA TGAACTGATT 
GTGGTTGGCC ATTCGCTTGG AGCTGGAACA GCTGCCATCT TGTCCTTCCT GTTACGCCCG 
CAGTATCCTT CCCTGAAGTG CTTTGCATAT TCCCCACCTG GTGGCTTGTT AAGTGAGGAT 
GCGATGGAAT ATTCTAAGGA ATTTGTTACC TCTGTGGTGC TTGGAAAGGA TCTAGTTCCA 
AGAATCGGCC TCTCGCAGCT GGAGGGTTTT CGCAGGCACT TGCTTGATGT TCTTCAGAGA 
AGCAACAAGC CTAAGTGGCG CATCATACTT GGCGGAACCA AATGCATACC TAAATCAGAA 
CTCCCGGAAG AGTCAGAGGC TATAACGATA CCCAGTAATC GCCTATGGAC GCACCCCAGT 
GACCTGACCA TAGCACTTTC AGCTAGCACA CCCCTTTACC CACCGGGGCG TATCATTCAT 
GTGGTTCACA ACCATCCAGT TGAGCAATGC TGTTTCTGGA ATCAGGAAGA ACCCACCTAC 
TTTGCCATCT GGGGTGATAA TAAGGCATTT GAGGAGGTCA TAATCTCTCC AGCCATGTTG 
CATGAACATC TCCCACACGT GGTAATGGAA GGACTGAACA AGGTCCTAGA GAATTACAAC 
AAGGGGAAAA CCGCGCTGCT CTCCGCTGCC AAGGTCATGG TGAGTCCAAC GGAAGTGGAT 
CTGAACCCAG AGCTGATTTT CCAACAGCAA CCTCACCCGC CTCCCCCAAT CCTGGAGGTA 
ATTCCTGCCA TCATGCCTGG AGCCCACAAC AGGAGGAACA GCAGCACCAA GAGTAAGCAG 
TCAGACATCA GCCTGGAAGG TTTCTATGAC TCTCGCCTTC TGTCACCAAT TCTAAAAGAC 
CCAGTAGAGC TCCTTTTGAT GAGCACAAAA GATTGCCTGG CTGTATCTCT CCAAGATCGC 
AGGGCACCTC TCGCCACCAT GGAGAGCCTC TCAGATAATG AATCTGTTTA CAGCTTTGAT 
TCACGGCGCT CTTCTGGTTT CCGCAGTATA CGAGGCTCAC CGAGCCTGAG AGCAGTGTTG 
GAGCGCGATG AGAGTCATTG TTTCTTCATT GACCCCACGA TCCCTGAGGA AAACCCCTCT 
TTGAGCTCAC GGACAGAGCT TCTAGGAGCA GATGCGCTCT CAAAGCATTC GCAGGAGAGC 
CAGCCGACTG AGGTTGCTCA CAATAGTGGC AGATCTTCAC CAGGTGACCT GCCTCCTTGG 
GATGAGGAGG CCTTAGCAGG AGTAGAGAGC AGCAGGGCTC TCACACCAGC TAAAGAAGAA 
TTGGCACTGC AGAATGGGCG CTTGAATGAG GTACCTAGTC CCCAGGTGCT GGAGTTTGCA 
GAATTTATAG ACAGCCTTTT TAATCTGGAC AACAAAAGTG GTTCTCTGCA GGATCTTTAC 
CATGTGATGG TTTCAGATGG TACAGACCGT GATATACCTA AGTCAGTTAG TGACCAGGAA 
ATCCTACTTC GGGCAAAGTT TGAACCTAAT CTGGTGCCCA AGCCCCCTCG CCTCTTTGCT 
GGCTCTGCAG ATCCATCCTC TGGCATTTCA GTGTCCGCCT CCTTCCCTCT CAGCTCCTCT 
GGGGAGCTCA TGGACTTAAC GCCAACGGGT ATGAGCAGCC AGGAGTGCCT GACGCCAGAC 
CCCATCCGAA CTCCAACGCC AACAGGAGCC AGTAACGGCA AAAAGGAAGA GTTGCTTATT 
TCTGCTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948809.1
CDS	1206..4097
Translation	MIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLAQYYTSCNDVTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQASSWTRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRKTKYLDLKNAQEMQRYKEVCYYMLFALAAYGWPMYLIRNPTCGICRLAGACSSCCCLCSARPRFAPGVTIEEDNCCGCNAIAIKRHFLDENMNMVDIVYTSCHDAVYETPFFVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLARGTKHYELIVVGHSLGAGTAAILSFLLRPQYPSLKCFAYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLDVLQRSNKPKWRIILGGTKCIPKSELPEESEAITIPSNRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPVEQCCFWNQEEPTYFAIWGDNKAFEEVIISPAMLHEHLPHVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLNPELIFQQQPHPPPPILEVIPAIMPGAHNRRNSSTKSKQSDISLEGFYDSRLLSPILKDPVELLLMSTKDCLAVSLQDRRAPLATMESLSDNESVYSFDSRRSSGFRSIRGSPSLRAVLERDESHCFFIDPTIPEENPSLSSRTELLGADALSKHSQESQPTEVAHNSGRSSPGDLPPWDEEALAGVESSRALTPAKEELALQNGRLNEVPSPQVLEFAEFIDSLFNLDNKSGSLQDLYHVMVSDGTDRDIPKSVSDQEILLRAKFEPNLVPKPPRLFAGSADPSSGISVSASFPLSSSGELMDLTPTGMSSQECLTPDPIRTPTPTGASNGKKEELLISAL

Target ORF information:

RefSeq Version	XM_018093320.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093320.1

ORF Insert Sequence:

ATGATTGCAG AAATGGCCAT TATCTGGCTG AGCATGAGAG GGGGCATCCT ATACACCGAG 
CCTCGGGACT CCATGCAGTA TGTGCTCTAC GTGCGCCTGG CGATCCTGGT GATTGAGTTC 
ATATACGCAA TCGTGGGCAT TGTCTGGCTG GCTCAGTATT ACACGTCGTG CAATGATGTC 
ACTGCCAAAA ATGTCACACT GGGGATGGTG GTGTGTAACT GGGTGGTCAT ACTCAGTGTG 
TGCATCACAG TCCTCTGTGT GTTCGATCCA ACCGGAAGAA CTTTCGTGAA GCTGAGGGCA 
ACAAAAAGGA GACAGAGAAA CCTGCGTACA TACAACCTGC GGCACCGACT AGAAGAAGGC 
CAGGCCAGCA GCTGGACACG GCGCCTGAAG GTGTTTCTGT GCTGCACGCG CACTAAAGAC 
TCCCAGTCTG ATGCATACTC TGAGATCGCC TACTTATTTG CTGAGTTTTT CAGAGATCTT 
GACATTGTTC CATCAGACAT CATTGCTGGA CTGGTTCTAC TGCGCCAGCG TCAAAGGGCA 
AAACGCAACG CTGTTCTCGA TGAGGCAAAC AATGACATAT TGGCTTTTCT ATCTGGGATG 
CCCGTCACCA GGAAAACAAA ATACCTAGAC CTGAAAAATG CACAAGAGAT GCAGCGGTAC 
AAGGAGGTCT GTTACTACAT GCTCTTTGCG CTGGCAGCAT ATGGCTGGCC TATGTACCTC 
ATTAGGAACC CAACCTGTGG CATCTGCCGT TTGGCAGGGG CATGCTCCAG TTGCTGCTGC 
CTTTGCTCAG CAAGGCCGCG CTTTGCCCCC GGGGTCACAA TTGAGGAGGA TAACTGCTGC 
GGCTGTAACG CCATCGCCAT AAAACGCCAC TTCTTAGACG AGAACATGAA CATGGTGGAC 
ATTGTGTATA CTTCATGCCA TGATGCTGTT TATGAAACAC CATTCTTTGT TGCAGTTGAT 
CATGATAAGA AGAAAGTGGT GATTAGCATT CGAGGGACAC TCTCTCCCAA GGATGCCTTG 
ACAGATCTGA CAGGAGATGC GGAACGGTTG CCAGTGGAAG GGCATCATGG CACCTGGTTG 
GGACACAAGG GAATGGTTCT GTCAGCTGAA TACATTAAAA AGAAGCTGGA GCAAGAGATG 
GTTCTTTCCC AAGCATTTGG CCGTGACCTG GCACGTGGGA CCAAACACTA TGAACTGATT 
GTGGTTGGCC ATTCGCTTGG AGCTGGAACA GCTGCCATCT TGTCCTTCCT GTTACGCCCG 
CAGTATCCTT CCCTGAAGTG CTTTGCATAT TCCCCACCTG GTGGCTTGTT AAGTGAGGAT 
GCGATGGAAT ATTCTAAGGA ATTTGTTACC TCTGTGGTGC TTGGAAAGGA TCTAGTTCCA 
AGAATCGGCC TCTCGCAGCT GGAGGGTTTT CGCAGGCACT TGCTTGATGT TCTTCAGAGA 
AGCAACAAGC CTAAGTGGCG CATCATACTT GGCGGAACCA AATGCATACC TAAATCAGAA 
CTCCCGGAAG AGTCAGAGGC TATAACGATA CCCAGTAATC GCCTATGGAC GCACCCCAGT 
GACCTGACCA TAGCACTTTC AGCTAGCACA CCCCTTTACC CACCGGGGCG TATCATTCAT 
GTGGTTCACA ACCATCCAGT TGAGCAATGC TGTTTCTGGA ATCAGGAAGA ACCCACCTAC 
TTTGCCATCT GGGGTGATAA TAAGGCATTT GAGGAGGTCA TAATCTCTCC AGCCATGTTG 
CATGAACATC TCCCACACGT GGTAATGGAA GGACTGAACA AGGTCCTAGA GAATTACAAC 
AAGGGGAAAA CCGCGCTGCT CTCCGCTGCC AAGGTCATGG TGAGTCCAAC GGAAGTGGAT 
CTGAACCCAG AGCTGATTTT CCAACAGCAA CCTCACCCGC CTCCCCCAAT CCTGGAGGTA 
ATTCCTGCCA TCATGCCTGG AGCCCACAAC AGGAGGAACA GCAGCACCAA GAGTAAGCAG 
TCAGACATCA GCCTGGAAGG TTTCTATGAC TCTCGCCTTC TGTCACCAAT TCTAAAAGAC 
CCAGTAGAGC TCCTTTTGAT GAGCACAAAA GATTGCCTGG CTGTATCTCT CCAAGATCGC 
AGGGCACCTC TCGCCACCAT GGAGAGCCTC TCAGATAATG AATCTGTTTA CAGCTTTGAT 
TCACGGCGCT CTTCTGGTTT CCGCAGTATA CGAGGCTCAC CGAGCCTGAG AGCAGTGTTG 
GAGCGCGATG AGAGTCATTG TTTCTTCATT GACCCCACGA TCCCTGAGGA AAACCCCTCT 
TTGAGCTCAC GGACAGAGCT TCTAGGAGCA GATGCGCTCT CAAAGCATTC GCAGGAGAGC 
CAGCCGACTG AGGTTGCTCA CAATAGTGGC AGATCTTCAC CAGGTGACCT GCCTCCTTGG 
GATGAGGAGG CCTTAGCAGG AGTAGAGAGC AGCAGGGCTC TCACACCAGC TAAAGAAGAA 
TTGGCACTGC AGAATGGGCG CTTGAATGAG GTACCTAGTC CCCAGGTGCT GGAGTTTGCA 
GAATTTATAG ACAGCCTTTT TAATCTGGAC AACAAAAGTG GTTCTCTGCA GGATCTTTAC 
CATGTGATGG TTTCAGATGG TACAGACCGT GATATACCTA AGTCAGTTAG TGACCAGGAA 
ATCCTACTTC GGGCAAAGTT TGAACCTAAT CTGGTGCCCA AGCCCCCTCG CCTCTTTGCT 
GGCTCTGCAG ATCCATCCTC TGGCATTTCA GTGTCCGCCT CCTTCCCTCT CAGCTCCTCT 
GGGGAGCTCA TGGACTTAAC GCCAACGGGT ATGAGCAGCC AGGAGTGCCT GACGCCAGAC 
CCCATCCGAA CTCCAACGCC AACAGGAGCC AGTAACGGCA AAAAGGAAGA GTTGCTTATT 
TCTGCTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dagla ( XM_004913411.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913468.1 Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa08580
Clone ID Related Accession (Same CDS sequence)	XM_004913411.1
Accession Version	XM_004913411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sn1-specific diacylglycerol lipase alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCC CGAGAGCGAT CCTGGTGATT GAGTTCATAT ACGCAATCGT GGGCATTGTC 
TGGCTGGCTC AGTATTACAC GTCGTGCAAT GATGTCACTG CCAAAAATGT CACACTGGGG 
ATGGTGGTGT GTAACTGGGT GGTCATACTC AGTGTGTGCA TCACAGTCCT CTGTGTGTTC 
GATCCAACCG GAAGAACTTT CGTGAAGCTG AGGGCAACAA AAAGGAGACA GAGAAACCTG 
CGTACATACA ACCTGCGGCA CCGACTAGAA GAAGGCCAGG CCAGCAGCTG GACACGGCGC 
CTGAAGGTGT TTCTGTGCTG CACGCGCACT AAAGACTCCC AGTCTGATGC ATACTCTGAG 
ATCGCCTACT TATTTGCTGA GTTTTTCAGA GATCTTGACA TTGTTCCATC AGACATCATT 
GCTGGACTGG TTCTACTGCG CCAGCGTCAA AGGGCAAAAC GCAACGCTGT TCTCGATGAG 
GCAAACAATG ACATATTGGC TTTTCTATCT GGGATGCCCG TCACCAGGAA AACAAAATAC 
CTAGACCTGA AAAATGCACA AGAGATGCAG CGGTACAAGG AGGTCTGTTA CTACATGCTC 
TTTGCGCTGG CAGCATATGG CTGGCCTATG TACCTCATTA GGAACCCAAC CTGTGGCATC 
TGCCGTTTGG CAGGGGCATG CTCCAGTTGC TGCTGCCTTT GCTCAGCAAG GCCGCGCTTT 
GCCCCCGGGG TCACAATTGA GGAGGATAAC TGCTGCGGCT GTAACGCCAT CGCCATAAAA 
CGCCACTTCT TAGACGAGAA CATGAACATG GTGGACATTG TGTATACTTC ATGCCATGAT 
GCTGTTTATG AAACACCATT CTTTGTTGCA GTTGATCATG ATAAGAAGAA AGTGGTGATT 
AGCATTCGAG GGACACTCTC TCCCAAGGAT GCCTTGACAG ATCTGACAGG AGATGCGGAA 
CGGTTGCCAG TGGAAGGGCA TCATGGCACC TGGTTGGGAC ACAAGGGAAT GGTTCTGTCA 
GCTGAATACA TTAAAAAGAA GCTGGAGCAA GAGATGGTTC TTTCCCAAGC ATTTGGCCGT 
GACCTGGCAC GTGGGACCAA ACACTATGAA CTGATTGTGG TTGGCCATTC GCTTGGAGCT 
GGAACAGCTG CCATCTTGTC CTTCCTGTTA CGCCCGCAGT ATCCTTCCCT GAAGTGCTTT 
GCATATTCCC CACCTGGTGG CTTGTTAAGT GAGGATGCGA TGGAATATTC TAAGGAATTT 
GTTACCTCTG TGGTGCTTGG AAAGGATCTA GTTCCAAGAA TCGGCCTCTC GCAGCTGGAG 
GGTTTTCGCA GGCACTTGCT TGATGTTCTT CAGAGAAGCA ACAAGCCTAA GTGGCGCATC 
ATACTTGGCG GAACCAAATG CATACCTAAA TCAGAACTCC CGGAAGAGTC AGAGGCTATA 
ACGATACCCA GTAATCGCCT ATGGACGCAC CCCAGTGACC TGACCATAGC ACTTTCAGCT 
AGCACACCCC TTTACCCACC GGGGCGTATC ATTCATGTGG TTCACAACCA TCCAGTTGAG 
CAATGCTGTT TCTGGAATCA GGAAGAACCC ACCTACTTTG CCATCTGGGG TGATAATAAG 
GCATTTGAGG AGGTCATAAT CTCTCCAGCC ATGTTGCATG AACATCTCCC ACACGTGGTA 
ATGGAAGGAC TGAACAAGGT CCTAGAGAAT TACAACAAGG GGAAAACCGC GCTGCTCTCC 
GCTGCCAAGG TCATGGTGAG TCCAACGGAA GTGGATCTGA ACCCAGAGCT GATTTTCCAA 
CAGCAACCTC ACCCGCCTCC CCCAATCCTG GAGGTAATTC CTGCCATCAT GCCTGGAGCC 
CACAACAGGA GGAACAGCAG CACCAAGAGT AAGCAGTCAG ACATCAGCCT GGAAGGTTTC 
TATGACTCTC GCCTTCTGTC ACCAATTCTA AAAGACCCAG TAGAGCTCCT TTTGATGAGC 
ACAAAAGATT GCCTGGCTGT ATCTCTCCAA GATCGCAGGG CACCTCTCGC CACCATGGAG 
AGCCTCTCAG ATAATGAATC TGTTTACAGC TTTGATTCAC GGCGCTCTTC TGGTTTCCGC 
AGTATACGAG GCTCACCGAG CCTGAGAGCA GTGTTGGAGC GCGATGAGAG TCATTGTTTC 
TTCATTGACC CCACGATCCC TGAGGAAAAC CCCTCTTTGA GCTCACGGAC AGAGCTTCTA 
GGAGCAGATG CGCTCTCAAA GCATTCGCAG GAGAGCCAGC CGACTGAGGT TGCTCACAAT 
AGTGGCAGAT CTTCACCAGG TGACCTGCCT CCTTGGGATG AGGAGGCCTT AGCAGGAGTA 
GAGAGCAGCA GGGCTCTCAC ACCAGCTAAA GAAGAATTGG CACTGCAGAA TGGGCGCTTG 
AATGAGGTAC CTAGTCCCCA GGTGCTGGAG TTTGCAGAAT TTATAGACAG CCTTTTTAAT 
CTGGACAACA AAAGTGGTTC TCTGCAGGAT CTTTACCATG TGATGGTTTC AGATGGTACA 
GACCGTGATA TACCTAAGTC AGTTAGTGAC CAGGAAATCC TACTTCGGGC AAAGTTTGAA 
CCTAATCTGG TGCCCAAGCC CCCTCGCCTC TTTGCTGGCT CTGCAGATCC ATCCTCTGGC 
ATTTCAGTGT CCGCCTCCTT CCCTCTCAGC TCCTCTGGGG AGCTCATGGA CTTAACGCCA 
ACGGGTATGA GCAGCCAGGA GTGCCTGACG CCAGACCCCA TCCGAACTCC AACGCCAACA 
GGAGCCAGTA ACGGCAAAAA GGAAGAGTTG CTTATTTCTG CTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913468.1
CDS	1022..3829
Translation	MDRPRAILVIEFIYAIVGIVWLAQYYTSCNDVTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQASSWTRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRKTKYLDLKNAQEMQRYKEVCYYMLFALAAYGWPMYLIRNPTCGICRLAGACSSCCCLCSARPRFAPGVTIEEDNCCGCNAIAIKRHFLDENMNMVDIVYTSCHDAVYETPFFVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLARGTKHYELIVVGHSLGAGTAAILSFLLRPQYPSLKCFAYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLDVLQRSNKPKWRIILGGTKCIPKSELPEESEAITIPSNRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPVEQCCFWNQEEPTYFAIWGDNKAFEEVIISPAMLHEHLPHVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLNPELIFQQQPHPPPPILEVIPAIMPGAHNRRNSSTKSKQSDISLEGFYDSRLLSPILKDPVELLLMSTKDCLAVSLQDRRAPLATMESLSDNESVYSFDSRRSSGFRSIRGSPSLRAVLERDESHCFFIDPTIPEENPSLSSRTELLGADALSKHSQESQPTEVAHNSGRSSPGDLPPWDEEALAGVESSRALTPAKEELALQNGRLNEVPSPQVLEFAEFIDSLFNLDNKSGSLQDLYHVMVSDGTDRDIPKSVSDQEILLRAKFEPNLVPKPPRLFAGSADPSSGISVSASFPLSSSGELMDLTPTGMSSQECLTPDPIRTPTPTGASNGKKEELLISAL

Target ORF information:

RefSeq Version	XM_004913411.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913411.1

ORF Insert Sequence:

ATGGATCGCC CGAGAGCGAT CCTGGTGATT GAGTTCATAT ACGCAATCGT GGGCATTGTC 
TGGCTGGCTC AGTATTACAC GTCGTGCAAT GATGTCACTG CCAAAAATGT CACACTGGGG 
ATGGTGGTGT GTAACTGGGT GGTCATACTC AGTGTGTGCA TCACAGTCCT CTGTGTGTTC 
GATCCAACCG GAAGAACTTT CGTGAAGCTG AGGGCAACAA AAAGGAGACA GAGAAACCTG 
CGTACATACA ACCTGCGGCA CCGACTAGAA GAAGGCCAGG CCAGCAGCTG GACACGGCGC 
CTGAAGGTGT TTCTGTGCTG CACGCGCACT AAAGACTCCC AGTCTGATGC ATACTCTGAG 
ATCGCCTACT TATTTGCTGA GTTTTTCAGA GATCTTGACA TTGTTCCATC AGACATCATT 
GCTGGACTGG TTCTACTGCG CCAGCGTCAA AGGGCAAAAC GCAACGCTGT TCTCGATGAG 
GCAAACAATG ACATATTGGC TTTTCTATCT GGGATGCCCG TCACCAGGAA AACAAAATAC 
CTAGACCTGA AAAATGCACA AGAGATGCAG CGGTACAAGG AGGTCTGTTA CTACATGCTC 
TTTGCGCTGG CAGCATATGG CTGGCCTATG TACCTCATTA GGAACCCAAC CTGTGGCATC 
TGCCGTTTGG CAGGGGCATG CTCCAGTTGC TGCTGCCTTT GCTCAGCAAG GCCGCGCTTT 
GCCCCCGGGG TCACAATTGA GGAGGATAAC TGCTGCGGCT GTAACGCCAT CGCCATAAAA 
CGCCACTTCT TAGACGAGAA CATGAACATG GTGGACATTG TGTATACTTC ATGCCATGAT 
GCTGTTTATG AAACACCATT CTTTGTTGCA GTTGATCATG ATAAGAAGAA AGTGGTGATT 
AGCATTCGAG GGACACTCTC TCCCAAGGAT GCCTTGACAG ATCTGACAGG AGATGCGGAA 
CGGTTGCCAG TGGAAGGGCA TCATGGCACC TGGTTGGGAC ACAAGGGAAT GGTTCTGTCA 
GCTGAATACA TTAAAAAGAA GCTGGAGCAA GAGATGGTTC TTTCCCAAGC ATTTGGCCGT 
GACCTGGCAC GTGGGACCAA ACACTATGAA CTGATTGTGG TTGGCCATTC GCTTGGAGCT 
GGAACAGCTG CCATCTTGTC CTTCCTGTTA CGCCCGCAGT ATCCTTCCCT GAAGTGCTTT 
GCATATTCCC CACCTGGTGG CTTGTTAAGT GAGGATGCGA TGGAATATTC TAAGGAATTT 
GTTACCTCTG TGGTGCTTGG AAAGGATCTA GTTCCAAGAA TCGGCCTCTC GCAGCTGGAG 
GGTTTTCGCA GGCACTTGCT TGATGTTCTT CAGAGAAGCA ACAAGCCTAA GTGGCGCATC 
ATACTTGGCG GAACCAAATG CATACCTAAA TCAGAACTCC CGGAAGAGTC AGAGGCTATA 
ACGATACCCA GTAATCGCCT ATGGACGCAC CCCAGTGACC TGACCATAGC ACTTTCAGCT 
AGCACACCCC TTTACCCACC GGGGCGTATC ATTCATGTGG TTCACAACCA TCCAGTTGAG 
CAATGCTGTT TCTGGAATCA GGAAGAACCC ACCTACTTTG CCATCTGGGG TGATAATAAG 
GCATTTGAGG AGGTCATAAT CTCTCCAGCC ATGTTGCATG AACATCTCCC ACACGTGGTA 
ATGGAAGGAC TGAACAAGGT CCTAGAGAAT TACAACAAGG GGAAAACCGC GCTGCTCTCC 
GCTGCCAAGG TCATGGTGAG TCCAACGGAA GTGGATCTGA ACCCAGAGCT GATTTTCCAA 
CAGCAACCTC ACCCGCCTCC CCCAATCCTG GAGGTAATTC CTGCCATCAT GCCTGGAGCC 
CACAACAGGA GGAACAGCAG CACCAAGAGT AAGCAGTCAG ACATCAGCCT GGAAGGTTTC 
TATGACTCTC GCCTTCTGTC ACCAATTCTA AAAGACCCAG TAGAGCTCCT TTTGATGAGC 
ACAAAAGATT GCCTGGCTGT ATCTCTCCAA GATCGCAGGG CACCTCTCGC CACCATGGAG 
AGCCTCTCAG ATAATGAATC TGTTTACAGC TTTGATTCAC GGCGCTCTTC TGGTTTCCGC 
AGTATACGAG GCTCACCGAG CCTGAGAGCA GTGTTGGAGC GCGATGAGAG TCATTGTTTC 
TTCATTGACC CCACGATCCC TGAGGAAAAC CCCTCTTTGA GCTCACGGAC AGAGCTTCTA 
GGAGCAGATG CGCTCTCAAA GCATTCGCAG GAGAGCCAGC CGACTGAGGT TGCTCACAAT 
AGTGGCAGAT CTTCACCAGG TGACCTGCCT CCTTGGGATG AGGAGGCCTT AGCAGGAGTA 
GAGAGCAGCA GGGCTCTCAC ACCAGCTAAA GAAGAATTGG CACTGCAGAA TGGGCGCTTG 
AATGAGGTAC CTAGTCCCCA GGTGCTGGAG TTTGCAGAAT TTATAGACAG CCTTTTTAAT 
CTGGACAACA AAAGTGGTTC TCTGCAGGAT CTTTACCATG TGATGGTTTC AGATGGTACA 
GACCGTGATA TACCTAAGTC AGTTAGTGAC CAGGAAATCC TACTTCGGGC AAAGTTTGAA 
CCTAATCTGG TGCCCAAGCC CCCTCGCCTC TTTGCTGGCT CTGCAGATCC ATCCTCTGGC 
ATTTCAGTGT CCGCCTCCTT CCCTCTCAGC TCCTCTGGGG AGCTCATGGA CTTAACGCCA 
ACGGGTATGA GCAGCCAGGA GTGCCTGACG CCAGACCCCA TCCGAACTCC AACGCCAACA 
GGAGCCAGTA ACGGCAAAAA GGAAGAGTTG CTTATTTCTG CTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dagla ( XM_031900543.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756403.1 Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa56567
Clone ID Related Accession (Same CDS sequence)	XM_031900543.1 , XM_004913409.3 , XM_004913408.2
Accession Version	XM_031900543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sn1-specific diacylglycerol lipase alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGAA TTGTGGTTTT CCGCCGCCGC TGGTCTGTGG GCAGCGATGA TTTGGTGCTG 
CCCGGAATCT TCCTCTTCGT GCTGCATAGT ACCTGGTTTG TTATCCTGTC TGTGGTTCTC 
TTTGGACTGA CGTATAACCC ACACGAGACA TGCTCCCTCA ACTTGGTAGA TCATGGTCGG 
GGGTACCTGG GCATCCTGCT CAGCTGCATG ATTGCAGAAA TGGCCATTAT CTGGCTGAGC 
ATGAGAGGGG GCATCCTATA CACCGAGCCT CGGGACTCCA TGCAGTATGT GCTCTACGTG 
CGCCTGGCGA TCCTGGTGAT TGAGTTCATA TACGCAATCG TGGGCATTGT CTGGCTGGCT 
CAGTATTACA CGTCGTGCAA TGATGTCACT GCCAAAAATG TCACACTGGG GATGGTGGTG 
TGTAACTGGG TGGTCATACT CAGTGTGTGC ATCACAGTCC TCTGTGTGTT CGATCCAACC 
GGAAGAACTT TCGTGAAGCT GAGGGCAACA AAAAGGAGAC AGAGAAACCT GCGTACATAC 
AACCTGCGGC ACCGACTAGA AGAAGGCCAG GCCAGCAGCT GGACACGGCG CCTGAAGGTG 
TTTCTGTGCT GCACGCGCAC TAAAGACTCC CAGTCTGATG CATACTCTGA GATCGCCTAC 
TTATTTGCTG AGTTTTTCAG AGATCTTGAC ATTGTTCCAT CAGACATCAT TGCTGGACTG 
GTTCTACTGC GCCAGCGTCA AAGGGCAAAA CGCAACGCTG TTCTCGATGA GGCAAACAAT 
GACATATTGG CTTTTCTATC TGGGATGCCC GTCACCAGGA AAACAAAATA CCTAGACCTG 
AAAAATGCAC AAGAGATGCA GCGGTACAAG GAGGTCTGTT ACTACATGCT CTTTGCGCTG 
GCAGCATATG GCTGGCCTAT GTACCTCATT AGGAACCCAA CCTGTGGCAT CTGCCGTTTG 
GCAGGGGCAT GCTCCAGTTG CTGCTGCCTT TGCTCAGCAA GGCCGCGCTT TGCCCCCGGG 
GTCACAATTG AGGAGGATAA CTGCTGCGGC TGTAACGCCA TCGCCATAAA ACGCCACTTC 
TTAGACGAGA ACATGAACAT GGTGGACATT GTGTATACTT CATGCCATGA TGCTGTTTAT 
GAAACACCAT TCTTTGTTGC AGTTGATCAT GATAAGAAGA AAGTGGTGAT TAGCATTCGA 
GGGACACTCT CTCCCAAGGA TGCCTTGACA GATCTGACAG GAGATGCGGA ACGGTTGCCA 
GTGGAAGGGC ATCATGGCAC CTGGTTGGGA CACAAGGGAA TGGTTCTGTC AGCTGAATAC 
ATTAAAAAGA AGCTGGAGCA AGAGATGGTT CTTTCCCAAG CATTTGGCCG TGACCTGGCA 
CGTGGGACCA AACACTATGA ACTGATTGTG GTTGGCCATT CGCTTGGAGC TGGAACAGCT 
GCCATCTTGT CCTTCCTGTT ACGCCCGCAG TATCCTTCCC TGAAGTGCTT TGCATATTCC 
CCACCTGGTG GCTTGTTAAG TGAGGATGCG ATGGAATATT CTAAGGAATT TGTTACCTCT 
GTGGTGCTTG GAAAGGATCT AGTTCCAAGA ATCGGCCTCT CGCAGCTGGA GGGTTTTCGC 
AGGCACTTGC TTGATGTTCT TCAGAGAAGC AACAAGCCTA AGTGGCGCAT CATACTTGGC 
GGAACCAAAT GCATACCTAA ATCAGAACTC CCGGAAGAGT CAGAGGCTAT AACGATACCC 
AGTAATCGCC TATGGACGCA CCCTAGTGAC CTGACCATAG CACTTTCAGC TAGCACACCC 
CTTTACCCAC CGGGGCGTAT CATTCATGTG GTTCACAACC ATCCAGTTGA GCAATGCTGT 
TTCTGGAATC AGGAAGAACC CACCTACTTT GCCATCTGGG GTGATAATAA GGCATTTGAG 
GAGGTCATAA TCTCTCCAGC CATGTTGCAT GAACATCTCC CACACGTGGT AATGGAAGGA 
CTGAACAAGG TCCTAGAGAA TTACAACAAG GGGAAAACCG CGCTGCTCTC CGCTGCCAAG 
GTCATGGTGA GTCCAACGGA AGTGGATCTG AACCCAGAGC TGATTTTCCA ACAGCAACCT 
CACCCGCCTC CCCCAATCCT GGAGGTAATT CCTGCCATCA TGCCTGGAGC CCACAACAGG 
AGGAACAGCA GCACCAAGAG TAAGCAGTCA GACATCAGCC TGGAAGGTTT CTATGACTCT 
CGCCTTCTGT CACCAATTCT AAAAGACCCA GTAGAGCTCC TTTTGATGAG CACAAAAGAT 
TGCCTGGCTG TATCTCTCCA AGATCGCAGG GCACCTCTCG CCACCATGGA GAGCCTCTCA 
GATAATGAAT CTGTTTACAG CTTTGATTCA CGGCGCTCTT CTGGTTTCCG CAGTATACGA 
GGCTCACCGA GCCTGAGAGC AGTGTTGGAG CGCGATGAGA GTCATTGTTT CTTCATTGAC 
CCCACGATCC CTGAGGAAAA CCCCTCTTTG AGCTCACGGA CAGAGCTTCT AGGAGCAGAT 
GCGCTCTCAA AGCATTCGCA GGAGAGCCAG CCGACTGAGG TTGCTCACAA TAGTGGCAGA 
TCTTCACCAG GTGACCTGCC TCCTTGGGAT GAGGAGGCCT TAGCAGGAGT AGAGAGCAGC 
AGGGCTCTCA CACCAGCTAA AGAAGAATTG GCACTGCAGA ATGGGCGCTT GAATGAGGTA 
CCTAGTCCCC AGGTGCTGGA GTTTGCAGAA TTTATAGACA GCCTTTTTAA TCTGGACAAC 
AAAAGTGGTT CTCTGCAGGA TCTTTACCAT GTGATGGTTT CAGATGGTAC AGACCGTGAT 
ATACCTAAGT CAGTTAGTGA CCAGGAAATC CTACTTCGGG CAAAGTTTGA ACCTAATCTG 
GTGCCCAAGC CCCCTCGCCT CTTTGCTGGC TCTGCAGATC CATCCTCTGG CATTTCAGTG 
TCCGCCTCCT TCCCTCTCAG CTCCTCTGGG GAGCTCATGG ACTTAACGCC AACGGGTATG 
AGCAGCCAGG AGTGCCTGAC GCCAGACCCC ATCCGAACTC CAACGCCAAC AGGAGCCAGT 
AACGGCAAAA AGGAAGAGTT GCTTATTTCT GCTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756403.1
CDS	1084..4182
Translation	MPGIVVFRRRWSVGSDDLVLPGIFLFVLHSTWFVILSVVLFGLTYNPHETCSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLAQYYTSCNDVTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQASSWTRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRKTKYLDLKNAQEMQRYKEVCYYMLFALAAYGWPMYLIRNPTCGICRLAGACSSCCCLCSARPRFAPGVTIEEDNCCGCNAIAIKRHFLDENMNMVDIVYTSCHDAVYETPFFVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLARGTKHYELIVVGHSLGAGTAAILSFLLRPQYPSLKCFAYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLDVLQRSNKPKWRIILGGTKCIPKSELPEESEAITIPSNRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPVEQCCFWNQEEPTYFAIWGDNKAFEEVIISPAMLHEHLPHVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLNPELIFQQQPHPPPPILEVIPAIMPGAHNRRNSSTKSKQSDISLEGFYDSRLLSPILKDPVELLLMSTKDCLAVSLQDRRAPLATMESLSDNESVYSFDSRRSSGFRSIRGSPSLRAVLERDESHCFFIDPTIPEENPSLSSRTELLGADALSKHSQESQPTEVAHNSGRSSPGDLPPWDEEALAGVESSRALTPAKEELALQNGRLNEVPSPQVLEFAEFIDSLFNLDNKSGSLQDLYHVMVSDGTDRDIPKSVSDQEILLRAKFEPNLVPKPPRLFAGSADPSSGISVSASFPLSSSGELMDLTPTGMSSQECLTPDPIRTPTPTGASNGKKEELLISAL

Target ORF information:

RefSeq Version	XM_031900543.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900543.1

ORF Insert Sequence:

ATGCCAGGAA TTGTGGTTTT CCGCCGCCGC TGGTCTGTGG GCAGCGATGA TTTGGTGCTG 
CCCGGAATCT TCCTCTTCGT GCTGCATAGT ACCTGGTTTG TTATCCTGTC TGTGGTTCTC 
TTTGGACTGA CGTATAACCC ACACGAGACA TGCTCCCTCA ACTTGGTAGA TCATGGTCGG 
GGGTACCTGG GCATCCTGCT CAGCTGCATG ATTGCAGAAA TGGCCATTAT CTGGCTGAGC 
ATGAGAGGGG GCATCCTATA CACCGAGCCT CGGGACTCCA TGCAGTATGT GCTCTACGTG 
CGCCTGGCGA TCCTGGTGAT TGAGTTCATA TACGCAATCG TGGGCATTGT CTGGCTGGCT 
CAGTATTACA CGTCGTGCAA TGATGTCACT GCCAAAAATG TCACACTGGG GATGGTGGTG 
TGTAACTGGG TGGTCATACT CAGTGTGTGC ATCACAGTCC TCTGTGTGTT CGATCCAACC 
GGAAGAACTT TCGTGAAGCT GAGGGCAACA AAAAGGAGAC AGAGAAACCT GCGTACATAC 
AACCTGCGGC ACCGACTAGA AGAAGGCCAG GCCAGCAGCT GGACACGGCG CCTGAAGGTG 
TTTCTGTGCT GCACGCGCAC TAAAGACTCC CAGTCTGATG CATACTCTGA GATCGCCTAC 
TTATTTGCTG AGTTTTTCAG AGATCTTGAC ATTGTTCCAT CAGACATCAT TGCTGGACTG 
GTTCTACTGC GCCAGCGTCA AAGGGCAAAA CGCAACGCTG TTCTCGATGA GGCAAACAAT 
GACATATTGG CTTTTCTATC TGGGATGCCC GTCACCAGGA AAACAAAATA CCTAGACCTG 
AAAAATGCAC AAGAGATGCA GCGGTACAAG GAGGTCTGTT ACTACATGCT CTTTGCGCTG 
GCAGCATATG GCTGGCCTAT GTACCTCATT AGGAACCCAA CCTGTGGCAT CTGCCGTTTG 
GCAGGGGCAT GCTCCAGTTG CTGCTGCCTT TGCTCAGCAA GGCCGCGCTT TGCCCCCGGG 
GTCACAATTG AGGAGGATAA CTGCTGCGGC TGTAACGCCA TCGCCATAAA ACGCCACTTC 
TTAGACGAGA ACATGAACAT GGTGGACATT GTGTATACTT CATGCCATGA TGCTGTTTAT 
GAAACACCAT TCTTTGTTGC AGTTGATCAT GATAAGAAGA AAGTGGTGAT TAGCATTCGA 
GGGACACTCT CTCCCAAGGA TGCCTTGACA GATCTGACAG GAGATGCGGA ACGGTTGCCA 
GTGGAAGGGC ATCATGGCAC CTGGTTGGGA CACAAGGGAA TGGTTCTGTC AGCTGAATAC 
ATTAAAAAGA AGCTGGAGCA AGAGATGGTT CTTTCCCAAG CATTTGGCCG TGACCTGGCA 
CGTGGGACCA AACACTATGA ACTGATTGTG GTTGGCCATT CGCTTGGAGC TGGAACAGCT 
GCCATCTTGT CCTTCCTGTT ACGCCCGCAG TATCCTTCCC TGAAGTGCTT TGCATATTCC 
CCACCTGGTG GCTTGTTAAG TGAGGATGCG ATGGAATATT CTAAGGAATT TGTTACCTCT 
GTGGTGCTTG GAAAGGATCT AGTTCCAAGA ATCGGCCTCT CGCAGCTGGA GGGTTTTCGC 
AGGCACTTGC TTGATGTTCT TCAGAGAAGC AACAAGCCTA AGTGGCGCAT CATACTTGGC 
GGAACCAAAT GCATACCTAA ATCAGAACTC CCGGAAGAGT CAGAGGCTAT AACGATACCC 
AGTAATCGCC TATGGACGCA CCCTAGTGAC CTGACCATAG CACTTTCAGC TAGCACACCC 
CTTTACCCAC CGGGGCGTAT CATTCATGTG GTTCACAACC ATCCAGTTGA GCAATGCTGT 
TTCTGGAATC AGGAAGAACC CACCTACTTT GCCATCTGGG GTGATAATAA GGCATTTGAG 
GAGGTCATAA TCTCTCCAGC CATGTTGCAT GAACATCTCC CACACGTGGT AATGGAAGGA 
CTGAACAAGG TCCTAGAGAA TTACAACAAG GGGAAAACCG CGCTGCTCTC CGCTGCCAAG 
GTCATGGTGA GTCCAACGGA AGTGGATCTG AACCCAGAGC TGATTTTCCA ACAGCAACCT 
CACCCGCCTC CCCCAATCCT GGAGGTAATT CCTGCCATCA TGCCTGGAGC CCACAACAGG 
AGGAACAGCA GCACCAAGAG TAAGCAGTCA GACATCAGCC TGGAAGGTTT CTATGACTCT 
CGCCTTCTGT CACCAATTCT AAAAGACCCA GTAGAGCTCC TTTTGATGAG CACAAAAGAT 
TGCCTGGCTG TATCTCTCCA AGATCGCAGG GCACCTCTCG CCACCATGGA GAGCCTCTCA 
GATAATGAAT CTGTTTACAG CTTTGATTCA CGGCGCTCTT CTGGTTTCCG CAGTATACGA 
GGCTCACCGA GCCTGAGAGC AGTGTTGGAG CGCGATGAGA GTCATTGTTT CTTCATTGAC 
CCCACGATCC CTGAGGAAAA CCCCTCTTTG AGCTCACGGA CAGAGCTTCT AGGAGCAGAT 
GCGCTCTCAA AGCATTCGCA GGAGAGCCAG CCGACTGAGG TTGCTCACAA TAGTGGCAGA 
TCTTCACCAG GTGACCTGCC TCCTTGGGAT GAGGAGGCCT TAGCAGGAGT AGAGAGCAGC 
AGGGCTCTCA CACCAGCTAA AGAAGAATTG GCACTGCAGA ATGGGCGCTT GAATGAGGTA 
CCTAGTCCCC AGGTGCTGGA GTTTGCAGAA TTTATAGACA GCCTTTTTAA TCTGGACAAC 
AAAAGTGGTT CTCTGCAGGA TCTTTACCAT GTGATGGTTT CAGATGGTAC AGACCGTGAT 
ATACCTAAGT CAGTTAGTGA CCAGGAAATC CTACTTCGGG CAAAGTTTGA ACCTAATCTG 
GTGCCCAAGC CCCCTCGCCT CTTTGCTGGC TCTGCAGATC CATCCTCTGG CATTTCAGTG 
TCCGCCTCCT TCCCTCTCAG CTCCTCTGGG GAGCTCATGG ACTTAACGCC AACGGGTATG 
AGCAGCCAGG AGTGCCTGAC GCCAGACCCC ATCCGAACTC CAACGCCAAC AGGAGCCAGT 
AACGGCAAAA AGGAAGAGTT GCTTATTTCT GCTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dagla ( XM_004913409.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913466.1 Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa56567
Clone ID Related Accession (Same CDS sequence)	XM_031900543.1 , XM_004913409.3 , XM_004913408.2
Accession Version	XM_004913409.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sn1-specific diacylglycerol lipase alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913409.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGAA TTGTGGTTTT CCGCCGCCGC TGGTCTGTGG GCAGCGATGA TTTGGTGCTG 
CCCGGAATCT TCCTCTTCGT GCTGCATAGT ACCTGGTTTG TTATCCTGTC TGTGGTTCTC 
TTTGGACTGA CGTATAACCC ACACGAGACA TGCTCCCTCA ACTTGGTAGA TCATGGTCGG 
GGGTACCTGG GCATCCTGCT CAGCTGCATG ATTGCAGAAA TGGCCATTAT CTGGCTGAGC 
ATGAGAGGGG GCATCCTATA CACCGAGCCT CGGGACTCCA TGCAGTATGT GCTCTACGTG 
CGCCTGGCGA TCCTGGTGAT TGAGTTCATA TACGCAATCG TGGGCATTGT CTGGCTGGCT 
CAGTATTACA CGTCGTGCAA TGATGTCACT GCCAAAAATG TCACACTGGG GATGGTGGTG 
TGTAACTGGG TGGTCATACT CAGTGTGTGC ATCACAGTCC TCTGTGTGTT CGATCCAACC 
GGAAGAACTT TCGTGAAGCT GAGGGCAACA AAAAGGAGAC AGAGAAACCT GCGTACATAC 
AACCTGCGGC ACCGACTAGA AGAAGGCCAG GCCAGCAGCT GGACACGGCG CCTGAAGGTG 
TTTCTGTGCT GCACGCGCAC TAAAGACTCC CAGTCTGATG CATACTCTGA GATCGCCTAC 
TTATTTGCTG AGTTTTTCAG AGATCTTGAC ATTGTTCCAT CAGACATCAT TGCTGGACTG 
GTTCTACTGC GCCAGCGTCA AAGGGCAAAA CGCAACGCTG TTCTCGATGA GGCAAACAAT 
GACATATTGG CTTTTCTATC TGGGATGCCC GTCACCAGGA AAACAAAATA CCTAGACCTG 
AAAAATGCAC AAGAGATGCA GCGGTACAAG GAGGTCTGTT ACTACATGCT CTTTGCGCTG 
GCAGCATATG GCTGGCCTAT GTACCTCATT AGGAACCCAA CCTGTGGCAT CTGCCGTTTG 
GCAGGGGCAT GCTCCAGTTG CTGCTGCCTT TGCTCAGCAA GGCCGCGCTT TGCCCCCGGG 
GTCACAATTG AGGAGGATAA CTGCTGCGGC TGTAACGCCA TCGCCATAAA ACGCCACTTC 
TTAGACGAGA ACATGAACAT GGTGGACATT GTGTATACTT CATGCCATGA TGCTGTTTAT 
GAAACACCAT TCTTTGTTGC AGTTGATCAT GATAAGAAGA AAGTGGTGAT TAGCATTCGA 
GGGACACTCT CTCCCAAGGA TGCCTTGACA GATCTGACAG GAGATGCGGA ACGGTTGCCA 
GTGGAAGGGC ATCATGGCAC CTGGTTGGGA CACAAGGGAA TGGTTCTGTC AGCTGAATAC 
ATTAAAAAGA AGCTGGAGCA AGAGATGGTT CTTTCCCAAG CATTTGGCCG TGACCTGGCA 
CGTGGGACCA AACACTATGA ACTGATTGTG GTTGGCCATT CGCTTGGAGC TGGAACAGCT 
GCCATCTTGT CCTTCCTGTT ACGCCCGCAG TATCCTTCCC TGAAGTGCTT TGCATATTCC 
CCACCTGGTG GCTTGTTAAG TGAGGATGCG ATGGAATATT CTAAGGAATT TGTTACCTCT 
GTGGTGCTTG GAAAGGATCT AGTTCCAAGA ATCGGCCTCT CGCAGCTGGA GGGTTTTCGC 
AGGCACTTGC TTGATGTTCT TCAGAGAAGC AACAAGCCTA AGTGGCGCAT CATACTTGGC 
GGAACCAAAT GCATACCTAA ATCAGAACTC CCGGAAGAGT CAGAGGCTAT AACGATACCC 
AGTAATCGCC TATGGACGCA CCCTAGTGAC CTGACCATAG CACTTTCAGC TAGCACACCC 
CTTTACCCAC CGGGGCGTAT CATTCATGTG GTTCACAACC ATCCAGTTGA GCAATGCTGT 
TTCTGGAATC AGGAAGAACC CACCTACTTT GCCATCTGGG GTGATAATAA GGCATTTGAG 
GAGGTCATAA TCTCTCCAGC CATGTTGCAT GAACATCTCC CACACGTGGT AATGGAAGGA 
CTGAACAAGG TCCTAGAGAA TTACAACAAG GGGAAAACCG CGCTGCTCTC CGCTGCCAAG 
GTCATGGTGA GTCCAACGGA AGTGGATCTG AACCCAGAGC TGATTTTCCA ACAGCAACCT 
CACCCGCCTC CCCCAATCCT GGAGGTAATT CCTGCCATCA TGCCTGGAGC CCACAACAGG 
AGGAACAGCA GCACCAAGAG TAAGCAGTCA GACATCAGCC TGGAAGGTTT CTATGACTCT 
CGCCTTCTGT CACCAATTCT AAAAGACCCA GTAGAGCTCC TTTTGATGAG CACAAAAGAT 
TGCCTGGCTG TATCTCTCCA AGATCGCAGG GCACCTCTCG CCACCATGGA GAGCCTCTCA 
GATAATGAAT CTGTTTACAG CTTTGATTCA CGGCGCTCTT CTGGTTTCCG CAGTATACGA 
GGCTCACCGA GCCTGAGAGC AGTGTTGGAG CGCGATGAGA GTCATTGTTT CTTCATTGAC 
CCCACGATCC CTGAGGAAAA CCCCTCTTTG AGCTCACGGA CAGAGCTTCT AGGAGCAGAT 
GCGCTCTCAA AGCATTCGCA GGAGAGCCAG CCGACTGAGG TTGCTCACAA TAGTGGCAGA 
TCTTCACCAG GTGACCTGCC TCCTTGGGAT GAGGAGGCCT TAGCAGGAGT AGAGAGCAGC 
AGGGCTCTCA CACCAGCTAA AGAAGAATTG GCACTGCAGA ATGGGCGCTT GAATGAGGTA 
CCTAGTCCCC AGGTGCTGGA GTTTGCAGAA TTTATAGACA GCCTTTTTAA TCTGGACAAC 
AAAAGTGGTT CTCTGCAGGA TCTTTACCAT GTGATGGTTT CAGATGGTAC AGACCGTGAT 
ATACCTAAGT CAGTTAGTGA CCAGGAAATC CTACTTCGGG CAAAGTTTGA ACCTAATCTG 
GTGCCCAAGC CCCCTCGCCT CTTTGCTGGC TCTGCAGATC CATCCTCTGG CATTTCAGTG 
TCCGCCTCCT TCCCTCTCAG CTCCTCTGGG GAGCTCATGG ACTTAACGCC AACGGGTATG 
AGCAGCCAGG AGTGCCTGAC GCCAGACCCC ATCCGAACTC CAACGCCAAC AGGAGCCAGT 
AACGGCAAAA AGGAAGAGTT GCTTATTTCT GCTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913466.1
CDS	140..3238
Translation	MPGIVVFRRRWSVGSDDLVLPGIFLFVLHSTWFVILSVVLFGLTYNPHETCSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLAQYYTSCNDVTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQASSWTRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRKTKYLDLKNAQEMQRYKEVCYYMLFALAAYGWPMYLIRNPTCGICRLAGACSSCCCLCSARPRFAPGVTIEEDNCCGCNAIAIKRHFLDENMNMVDIVYTSCHDAVYETPFFVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLARGTKHYELIVVGHSLGAGTAAILSFLLRPQYPSLKCFAYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLDVLQRSNKPKWRIILGGTKCIPKSELPEESEAITIPSNRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPVEQCCFWNQEEPTYFAIWGDNKAFEEVIISPAMLHEHLPHVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLNPELIFQQQPHPPPPILEVIPAIMPGAHNRRNSSTKSKQSDISLEGFYDSRLLSPILKDPVELLLMSTKDCLAVSLQDRRAPLATMESLSDNESVYSFDSRRSSGFRSIRGSPSLRAVLERDESHCFFIDPTIPEENPSLSSRTELLGADALSKHSQESQPTEVAHNSGRSSPGDLPPWDEEALAGVESSRALTPAKEELALQNGRLNEVPSPQVLEFAEFIDSLFNLDNKSGSLQDLYHVMVSDGTDRDIPKSVSDQEILLRAKFEPNLVPKPPRLFAGSADPSSGISVSASFPLSSSGELMDLTPTGMSSQECLTPDPIRTPTPTGASNGKKEELLISAL

Target ORF information:

RefSeq Version	XM_004913409.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913409.3

ORF Insert Sequence:

ATGCCAGGAA TTGTGGTTTT CCGCCGCCGC TGGTCTGTGG GCAGCGATGA TTTGGTGCTG 
CCCGGAATCT TCCTCTTCGT GCTGCATAGT ACCTGGTTTG TTATCCTGTC TGTGGTTCTC 
TTTGGACTGA CGTATAACCC ACACGAGACA TGCTCCCTCA ACTTGGTAGA TCATGGTCGG 
GGGTACCTGG GCATCCTGCT CAGCTGCATG ATTGCAGAAA TGGCCATTAT CTGGCTGAGC 
ATGAGAGGGG GCATCCTATA CACCGAGCCT CGGGACTCCA TGCAGTATGT GCTCTACGTG 
CGCCTGGCGA TCCTGGTGAT TGAGTTCATA TACGCAATCG TGGGCATTGT CTGGCTGGCT 
CAGTATTACA CGTCGTGCAA TGATGTCACT GCCAAAAATG TCACACTGGG GATGGTGGTG 
TGTAACTGGG TGGTCATACT CAGTGTGTGC ATCACAGTCC TCTGTGTGTT CGATCCAACC 
GGAAGAACTT TCGTGAAGCT GAGGGCAACA AAAAGGAGAC AGAGAAACCT GCGTACATAC 
AACCTGCGGC ACCGACTAGA AGAAGGCCAG GCCAGCAGCT GGACACGGCG CCTGAAGGTG 
TTTCTGTGCT GCACGCGCAC TAAAGACTCC CAGTCTGATG CATACTCTGA GATCGCCTAC 
TTATTTGCTG AGTTTTTCAG AGATCTTGAC ATTGTTCCAT CAGACATCAT TGCTGGACTG 
GTTCTACTGC GCCAGCGTCA AAGGGCAAAA CGCAACGCTG TTCTCGATGA GGCAAACAAT 
GACATATTGG CTTTTCTATC TGGGATGCCC GTCACCAGGA AAACAAAATA CCTAGACCTG 
AAAAATGCAC AAGAGATGCA GCGGTACAAG GAGGTCTGTT ACTACATGCT CTTTGCGCTG 
GCAGCATATG GCTGGCCTAT GTACCTCATT AGGAACCCAA CCTGTGGCAT CTGCCGTTTG 
GCAGGGGCAT GCTCCAGTTG CTGCTGCCTT TGCTCAGCAA GGCCGCGCTT TGCCCCCGGG 
GTCACAATTG AGGAGGATAA CTGCTGCGGC TGTAACGCCA TCGCCATAAA ACGCCACTTC 
TTAGACGAGA ACATGAACAT GGTGGACATT GTGTATACTT CATGCCATGA TGCTGTTTAT 
GAAACACCAT TCTTTGTTGC AGTTGATCAT GATAAGAAGA AAGTGGTGAT TAGCATTCGA 
GGGACACTCT CTCCCAAGGA TGCCTTGACA GATCTGACAG GAGATGCGGA ACGGTTGCCA 
GTGGAAGGGC ATCATGGCAC CTGGTTGGGA CACAAGGGAA TGGTTCTGTC AGCTGAATAC 
ATTAAAAAGA AGCTGGAGCA AGAGATGGTT CTTTCCCAAG CATTTGGCCG TGACCTGGCA 
CGTGGGACCA AACACTATGA ACTGATTGTG GTTGGCCATT CGCTTGGAGC TGGAACAGCT 
GCCATCTTGT CCTTCCTGTT ACGCCCGCAG TATCCTTCCC TGAAGTGCTT TGCATATTCC 
CCACCTGGTG GCTTGTTAAG TGAGGATGCG ATGGAATATT CTAAGGAATT TGTTACCTCT 
GTGGTGCTTG GAAAGGATCT AGTTCCAAGA ATCGGCCTCT CGCAGCTGGA GGGTTTTCGC 
AGGCACTTGC TTGATGTTCT TCAGAGAAGC AACAAGCCTA AGTGGCGCAT CATACTTGGC 
GGAACCAAAT GCATACCTAA ATCAGAACTC CCGGAAGAGT CAGAGGCTAT AACGATACCC 
AGTAATCGCC TATGGACGCA CCCTAGTGAC CTGACCATAG CACTTTCAGC TAGCACACCC 
CTTTACCCAC CGGGGCGTAT CATTCATGTG GTTCACAACC ATCCAGTTGA GCAATGCTGT 
TTCTGGAATC AGGAAGAACC CACCTACTTT GCCATCTGGG GTGATAATAA GGCATTTGAG 
GAGGTCATAA TCTCTCCAGC CATGTTGCAT GAACATCTCC CACACGTGGT AATGGAAGGA 
CTGAACAAGG TCCTAGAGAA TTACAACAAG GGGAAAACCG CGCTGCTCTC CGCTGCCAAG 
GTCATGGTGA GTCCAACGGA AGTGGATCTG AACCCAGAGC TGATTTTCCA ACAGCAACCT 
CACCCGCCTC CCCCAATCCT GGAGGTAATT CCTGCCATCA TGCCTGGAGC CCACAACAGG 
AGGAACAGCA GCACCAAGAG TAAGCAGTCA GACATCAGCC TGGAAGGTTT CTATGACTCT 
CGCCTTCTGT CACCAATTCT AAAAGACCCA GTAGAGCTCC TTTTGATGAG CACAAAAGAT 
TGCCTGGCTG TATCTCTCCA AGATCGCAGG GCACCTCTCG CCACCATGGA GAGCCTCTCA 
GATAATGAAT CTGTTTACAG CTTTGATTCA CGGCGCTCTT CTGGTTTCCG CAGTATACGA 
GGCTCACCGA GCCTGAGAGC AGTGTTGGAG CGCGATGAGA GTCATTGTTT CTTCATTGAC 
CCCACGATCC CTGAGGAAAA CCCCTCTTTG AGCTCACGGA CAGAGCTTCT AGGAGCAGAT 
GCGCTCTCAA AGCATTCGCA GGAGAGCCAG CCGACTGAGG TTGCTCACAA TAGTGGCAGA 
TCTTCACCAG GTGACCTGCC TCCTTGGGAT GAGGAGGCCT TAGCAGGAGT AGAGAGCAGC 
AGGGCTCTCA CACCAGCTAA AGAAGAATTG GCACTGCAGA ATGGGCGCTT GAATGAGGTA 
CCTAGTCCCC AGGTGCTGGA GTTTGCAGAA TTTATAGACA GCCTTTTTAA TCTGGACAAC 
AAAAGTGGTT CTCTGCAGGA TCTTTACCAT GTGATGGTTT CAGATGGTAC AGACCGTGAT 
ATACCTAAGT CAGTTAGTGA CCAGGAAATC CTACTTCGGG CAAAGTTTGA ACCTAATCTG 
GTGCCCAAGC CCCCTCGCCT CTTTGCTGGC TCTGCAGATC CATCCTCTGG CATTTCAGTG 
TCCGCCTCCT TCCCTCTCAG CTCCTCTGGG GAGCTCATGG ACTTAACGCC AACGGGTATG 
AGCAGCCAGG AGTGCCTGAC GCCAGACCCC ATCCGAACTC CAACGCCAAC AGGAGCCAGT 
AACGGCAAAA AGGAAGAGTT GCTTATTTCT GCTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dagla ( XM_004913408.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913465.1 Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa56567
Clone ID Related Accession (Same CDS sequence)	XM_031900543.1 , XM_004913409.3 , XM_004913408.2
Accession Version	XM_004913408.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sn1-specific diacylglycerol lipase alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913408.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGAA TTGTGGTTTT CCGCCGCCGC TGGTCTGTGG GCAGCGATGA TTTGGTGCTG 
CCCGGAATCT TCCTCTTCGT GCTGCATAGT ACCTGGTTTG TTATCCTGTC TGTGGTTCTC 
TTTGGACTGA CGTATAACCC ACACGAGACA TGCTCCCTCA ACTTGGTAGA TCATGGTCGG 
GGGTACCTGG GCATCCTGCT CAGCTGCATG ATTGCAGAAA TGGCCATTAT CTGGCTGAGC 
ATGAGAGGGG GCATCCTATA CACCGAGCCT CGGGACTCCA TGCAGTATGT GCTCTACGTG 
CGCCTGGCGA TCCTGGTGAT TGAGTTCATA TACGCAATCG TGGGCATTGT CTGGCTGGCT 
CAGTATTACA CGTCGTGCAA TGATGTCACT GCCAAAAATG TCACACTGGG GATGGTGGTG 
TGTAACTGGG TGGTCATACT CAGTGTGTGC ATCACAGTCC TCTGTGTGTT CGATCCAACC 
GGAAGAACTT TCGTGAAGCT GAGGGCAACA AAAAGGAGAC AGAGAAACCT GCGTACATAC 
AACCTGCGGC ACCGACTAGA AGAAGGCCAG GCCAGCAGCT GGACACGGCG CCTGAAGGTG 
TTTCTGTGCT GCACGCGCAC TAAAGACTCC CAGTCTGATG CATACTCTGA GATCGCCTAC 
TTATTTGCTG AGTTTTTCAG AGATCTTGAC ATTGTTCCAT CAGACATCAT TGCTGGACTG 
GTTCTACTGC GCCAGCGTCA AAGGGCAAAA CGCAACGCTG TTCTCGATGA GGCAAACAAT 
GACATATTGG CTTTTCTATC TGGGATGCCC GTCACCAGGA AAACAAAATA CCTAGACCTG 
AAAAATGCAC AAGAGATGCA GCGGTACAAG GAGGTCTGTT ACTACATGCT CTTTGCGCTG 
GCAGCATATG GCTGGCCTAT GTACCTCATT AGGAACCCAA CCTGTGGCAT CTGCCGTTTG 
GCAGGGGCAT GCTCCAGTTG CTGCTGCCTT TGCTCAGCAA GGCCGCGCTT TGCCCCCGGG 
GTCACAATTG AGGAGGATAA CTGCTGCGGC TGTAACGCCA TCGCCATAAA ACGCCACTTC 
TTAGACGAGA ACATGAACAT GGTGGACATT GTGTATACTT CATGCCATGA TGCTGTTTAT 
GAAACACCAT TCTTTGTTGC AGTTGATCAT GATAAGAAGA AAGTGGTGAT TAGCATTCGA 
GGGACACTCT CTCCCAAGGA TGCCTTGACA GATCTGACAG GAGATGCGGA ACGGTTGCCA 
GTGGAAGGGC ATCATGGCAC CTGGTTGGGA CACAAGGGAA TGGTTCTGTC AGCTGAATAC 
ATTAAAAAGA AGCTGGAGCA AGAGATGGTT CTTTCCCAAG CATTTGGCCG TGACCTGGCA 
CGTGGGACCA AACACTATGA ACTGATTGTG GTTGGCCATT CGCTTGGAGC TGGAACAGCT 
GCCATCTTGT CCTTCCTGTT ACGCCCGCAG TATCCTTCCC TGAAGTGCTT TGCATATTCC 
CCACCTGGTG GCTTGTTAAG TGAGGATGCG ATGGAATATT CTAAGGAATT TGTTACCTCT 
GTGGTGCTTG GAAAGGATCT AGTTCCAAGA ATCGGCCTCT CGCAGCTGGA GGGTTTTCGC 
AGGCACTTGC TTGATGTTCT TCAGAGAAGC AACAAGCCTA AGTGGCGCAT CATACTTGGC 
GGAACCAAAT GCATACCTAA ATCAGAACTC CCGGAAGAGT CAGAGGCTAT AACGATACCC 
AGTAATCGCC TATGGACGCA CCCTAGTGAC CTGACCATAG CACTTTCAGC TAGCACACCC 
CTTTACCCAC CGGGGCGTAT CATTCATGTG GTTCACAACC ATCCAGTTGA GCAATGCTGT 
TTCTGGAATC AGGAAGAACC CACCTACTTT GCCATCTGGG GTGATAATAA GGCATTTGAG 
GAGGTCATAA TCTCTCCAGC CATGTTGCAT GAACATCTCC CACACGTGGT AATGGAAGGA 
CTGAACAAGG TCCTAGAGAA TTACAACAAG GGGAAAACCG CGCTGCTCTC CGCTGCCAAG 
GTCATGGTGA GTCCAACGGA AGTGGATCTG AACCCAGAGC TGATTTTCCA ACAGCAACCT 
CACCCGCCTC CCCCAATCCT GGAGGTAATT CCTGCCATCA TGCCTGGAGC CCACAACAGG 
AGGAACAGCA GCACCAAGAG TAAGCAGTCA GACATCAGCC TGGAAGGTTT CTATGACTCT 
CGCCTTCTGT CACCAATTCT AAAAGACCCA GTAGAGCTCC TTTTGATGAG CACAAAAGAT 
TGCCTGGCTG TATCTCTCCA AGATCGCAGG GCACCTCTCG CCACCATGGA GAGCCTCTCA 
GATAATGAAT CTGTTTACAG CTTTGATTCA CGGCGCTCTT CTGGTTTCCG CAGTATACGA 
GGCTCACCGA GCCTGAGAGC AGTGTTGGAG CGCGATGAGA GTCATTGTTT CTTCATTGAC 
CCCACGATCC CTGAGGAAAA CCCCTCTTTG AGCTCACGGA CAGAGCTTCT AGGAGCAGAT 
GCGCTCTCAA AGCATTCGCA GGAGAGCCAG CCGACTGAGG TTGCTCACAA TAGTGGCAGA 
TCTTCACCAG GTGACCTGCC TCCTTGGGAT GAGGAGGCCT TAGCAGGAGT AGAGAGCAGC 
AGGGCTCTCA CACCAGCTAA AGAAGAATTG GCACTGCAGA ATGGGCGCTT GAATGAGGTA 
CCTAGTCCCC AGGTGCTGGA GTTTGCAGAA TTTATAGACA GCCTTTTTAA TCTGGACAAC 
AAAAGTGGTT CTCTGCAGGA TCTTTACCAT GTGATGGTTT CAGATGGTAC AGACCGTGAT 
ATACCTAAGT CAGTTAGTGA CCAGGAAATC CTACTTCGGG CAAAGTTTGA ACCTAATCTG 
GTGCCCAAGC CCCCTCGCCT CTTTGCTGGC TCTGCAGATC CATCCTCTGG CATTTCAGTG 
TCCGCCTCCT TCCCTCTCAG CTCCTCTGGG GAGCTCATGG ACTTAACGCC AACGGGTATG 
AGCAGCCAGG AGTGCCTGAC GCCAGACCCC ATCCGAACTC CAACGCCAAC AGGAGCCAGT 
AACGGCAAAA AGGAAGAGTT GCTTATTTCT GCTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913465.1
CDS	955..4053
Translation	MPGIVVFRRRWSVGSDDLVLPGIFLFVLHSTWFVILSVVLFGLTYNPHETCSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLAQYYTSCNDVTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQASSWTRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRKTKYLDLKNAQEMQRYKEVCYYMLFALAAYGWPMYLIRNPTCGICRLAGACSSCCCLCSARPRFAPGVTIEEDNCCGCNAIAIKRHFLDENMNMVDIVYTSCHDAVYETPFFVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLARGTKHYELIVVGHSLGAGTAAILSFLLRPQYPSLKCFAYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLDVLQRSNKPKWRIILGGTKCIPKSELPEESEAITIPSNRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPVEQCCFWNQEEPTYFAIWGDNKAFEEVIISPAMLHEHLPHVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLNPELIFQQQPHPPPPILEVIPAIMPGAHNRRNSSTKSKQSDISLEGFYDSRLLSPILKDPVELLLMSTKDCLAVSLQDRRAPLATMESLSDNESVYSFDSRRSSGFRSIRGSPSLRAVLERDESHCFFIDPTIPEENPSLSSRTELLGADALSKHSQESQPTEVAHNSGRSSPGDLPPWDEEALAGVESSRALTPAKEELALQNGRLNEVPSPQVLEFAEFIDSLFNLDNKSGSLQDLYHVMVSDGTDRDIPKSVSDQEILLRAKFEPNLVPKPPRLFAGSADPSSGISVSASFPLSSSGELMDLTPTGMSSQECLTPDPIRTPTPTGASNGKKEELLISAL

Target ORF information:

RefSeq Version	XM_004913408.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913408.2

ORF Insert Sequence:

ATGCCAGGAA TTGTGGTTTT CCGCCGCCGC TGGTCTGTGG GCAGCGATGA TTTGGTGCTG 
CCCGGAATCT TCCTCTTCGT GCTGCATAGT ACCTGGTTTG TTATCCTGTC TGTGGTTCTC 
TTTGGACTGA CGTATAACCC ACACGAGACA TGCTCCCTCA ACTTGGTAGA TCATGGTCGG 
GGGTACCTGG GCATCCTGCT CAGCTGCATG ATTGCAGAAA TGGCCATTAT CTGGCTGAGC 
ATGAGAGGGG GCATCCTATA CACCGAGCCT CGGGACTCCA TGCAGTATGT GCTCTACGTG 
CGCCTGGCGA TCCTGGTGAT TGAGTTCATA TACGCAATCG TGGGCATTGT CTGGCTGGCT 
CAGTATTACA CGTCGTGCAA TGATGTCACT GCCAAAAATG TCACACTGGG GATGGTGGTG 
TGTAACTGGG TGGTCATACT CAGTGTGTGC ATCACAGTCC TCTGTGTGTT CGATCCAACC 
GGAAGAACTT TCGTGAAGCT GAGGGCAACA AAAAGGAGAC AGAGAAACCT GCGTACATAC 
AACCTGCGGC ACCGACTAGA AGAAGGCCAG GCCAGCAGCT GGACACGGCG CCTGAAGGTG 
TTTCTGTGCT GCACGCGCAC TAAAGACTCC CAGTCTGATG CATACTCTGA GATCGCCTAC 
TTATTTGCTG AGTTTTTCAG AGATCTTGAC ATTGTTCCAT CAGACATCAT TGCTGGACTG 
GTTCTACTGC GCCAGCGTCA AAGGGCAAAA CGCAACGCTG TTCTCGATGA GGCAAACAAT 
GACATATTGG CTTTTCTATC TGGGATGCCC GTCACCAGGA AAACAAAATA CCTAGACCTG 
AAAAATGCAC AAGAGATGCA GCGGTACAAG GAGGTCTGTT ACTACATGCT CTTTGCGCTG 
GCAGCATATG GCTGGCCTAT GTACCTCATT AGGAACCCAA CCTGTGGCAT CTGCCGTTTG 
GCAGGGGCAT GCTCCAGTTG CTGCTGCCTT TGCTCAGCAA GGCCGCGCTT TGCCCCCGGG 
GTCACAATTG AGGAGGATAA CTGCTGCGGC TGTAACGCCA TCGCCATAAA ACGCCACTTC 
TTAGACGAGA ACATGAACAT GGTGGACATT GTGTATACTT CATGCCATGA TGCTGTTTAT 
GAAACACCAT TCTTTGTTGC AGTTGATCAT GATAAGAAGA AAGTGGTGAT TAGCATTCGA 
GGGACACTCT CTCCCAAGGA TGCCTTGACA GATCTGACAG GAGATGCGGA ACGGTTGCCA 
GTGGAAGGGC ATCATGGCAC CTGGTTGGGA CACAAGGGAA TGGTTCTGTC AGCTGAATAC 
ATTAAAAAGA AGCTGGAGCA AGAGATGGTT CTTTCCCAAG CATTTGGCCG TGACCTGGCA 
CGTGGGACCA AACACTATGA ACTGATTGTG GTTGGCCATT CGCTTGGAGC TGGAACAGCT 
GCCATCTTGT CCTTCCTGTT ACGCCCGCAG TATCCTTCCC TGAAGTGCTT TGCATATTCC 
CCACCTGGTG GCTTGTTAAG TGAGGATGCG ATGGAATATT CTAAGGAATT TGTTACCTCT 
GTGGTGCTTG GAAAGGATCT AGTTCCAAGA ATCGGCCTCT CGCAGCTGGA GGGTTTTCGC 
AGGCACTTGC TTGATGTTCT TCAGAGAAGC AACAAGCCTA AGTGGCGCAT CATACTTGGC 
GGAACCAAAT GCATACCTAA ATCAGAACTC CCGGAAGAGT CAGAGGCTAT AACGATACCC 
AGTAATCGCC TATGGACGCA CCCTAGTGAC CTGACCATAG CACTTTCAGC TAGCACACCC 
CTTTACCCAC CGGGGCGTAT CATTCATGTG GTTCACAACC ATCCAGTTGA GCAATGCTGT 
TTCTGGAATC AGGAAGAACC CACCTACTTT GCCATCTGGG GTGATAATAA GGCATTTGAG 
GAGGTCATAA TCTCTCCAGC CATGTTGCAT GAACATCTCC CACACGTGGT AATGGAAGGA 
CTGAACAAGG TCCTAGAGAA TTACAACAAG GGGAAAACCG CGCTGCTCTC CGCTGCCAAG 
GTCATGGTGA GTCCAACGGA AGTGGATCTG AACCCAGAGC TGATTTTCCA ACAGCAACCT 
CACCCGCCTC CCCCAATCCT GGAGGTAATT CCTGCCATCA TGCCTGGAGC CCACAACAGG 
AGGAACAGCA GCACCAAGAG TAAGCAGTCA GACATCAGCC TGGAAGGTTT CTATGACTCT 
CGCCTTCTGT CACCAATTCT AAAAGACCCA GTAGAGCTCC TTTTGATGAG CACAAAAGAT 
TGCCTGGCTG TATCTCTCCA AGATCGCAGG GCACCTCTCG CCACCATGGA GAGCCTCTCA 
GATAATGAAT CTGTTTACAG CTTTGATTCA CGGCGCTCTT CTGGTTTCCG CAGTATACGA 
GGCTCACCGA GCCTGAGAGC AGTGTTGGAG CGCGATGAGA GTCATTGTTT CTTCATTGAC 
CCCACGATCC CTGAGGAAAA CCCCTCTTTG AGCTCACGGA CAGAGCTTCT AGGAGCAGAT 
GCGCTCTCAA AGCATTCGCA GGAGAGCCAG CCGACTGAGG TTGCTCACAA TAGTGGCAGA 
TCTTCACCAG GTGACCTGCC TCCTTGGGAT GAGGAGGCCT TAGCAGGAGT AGAGAGCAGC 
AGGGCTCTCA CACCAGCTAA AGAAGAATTG GCACTGCAGA ATGGGCGCTT GAATGAGGTA 
CCTAGTCCCC AGGTGCTGGA GTTTGCAGAA TTTATAGACA GCCTTTTTAA TCTGGACAAC 
AAAAGTGGTT CTCTGCAGGA TCTTTACCAT GTGATGGTTT CAGATGGTAC AGACCGTGAT 
ATACCTAAGT CAGTTAGTGA CCAGGAAATC CTACTTCGGG CAAAGTTTGA ACCTAATCTG 
GTGCCCAAGC CCCCTCGCCT CTTTGCTGGC TCTGCAGATC CATCCTCTGG CATTTCAGTG 
TCCGCCTCCT TCCCTCTCAG CTCCTCTGGG GAGCTCATGG ACTTAACGCC AACGGGTATG 
AGCAGCCAGG AGTGCCTGAC GCCAGACCCC ATCCGAACTC CAACGCCAAC AGGAGCCAGT 
AACGGCAAAA AGGAAGAGTT GCTTATTTCT GCTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dagla ( XM_004913409.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913466.1 Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X2, mRNA.

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CloneID	OXa08578
Clone ID Related Accession (Same CDS sequence)	XM_004913409.2 , XM_004913408.1
Accession Version	XM_004913409.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_004913409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGAA TTGTGGTTTT CCGCCGCCGC TGGTCTGTGG GCAGCGATGA TTTGGTGCTG 
CCCGGAATCT TCCTCTTCGT GCTGCATAGT ACCTGGTTTG TTATCCTGTC TGTGGTTCTC 
TTTGGACTGA CGTATAACCC ACACGAGACA TGCTCCCTCA ACTTGGTAGA TCATGGTCGG 
GGGTACCTGG GCATCCTGCT CAGCTGCATG ATTGCAGAAA TGGCCATTAT CTGGCTGAGC 
ATGAGAGGGG GCATCCTATA CACCGAGCCT CGGGACTCCA TGCAGTATGT GCTCTACGTG 
CGCCTGGCGA TCCTGGTGAT TGAGTTCATA TACGCAATCG TGGGCATTGT CTGGCTGGCT 
CAGTATTACA CGTCGTGCAA TGATGTCACT GCCAAAAATG TCACACTGGG GATGGTGGTG 
TGTAACTGGG TGGTCATACT CAGTGTGTGC ATCACAGTCC TCTGTGTGTT CGATCCAACC 
GGAAGAACTT TCGTGAAGCT GAGGGCAACA AAAAGGAGAC AGAGAAACCT GCGTACATAC 
AACCTGCGGC ACCGACTAGA AGAAGGCCAG GCCAGCAGCT GGACACGGCG CCTGAAGGTG 
TTTCTGTGCT GCACGCGCAC TAAAGACTCC CAGTCTGATG CATACTCTGA GATCGCCTAC 
TTATTTGCTG AGTTTTTCAG AGATCTTGAC ATTGTTCCAT CAGACATCAT TGCTGGACTG 
GTTCTACTGC GCCAGCGTCA AAGGGCAAAA CGCAACGCTG TTCTCGATGA GGCAAACAAT 
GACATATTGG CTTTTCTATC TGGGATGCCC GTCACCAGGA AAACAAAATA CCTAGACCTG 
AAAAATGCAC AAGAGATGCA GCGGTACAAG GAGGTCTGTT ACTACATGCT CTTTGCGCTG 
GCAGCATATG GCTGGCCTAT GTACCTCATT AGGAACCCAA CCTGTGGCAT CTGCCGTTTG 
GCAGGGGCAT GCTCCAGTTG CTGCTGCCTT TGCTCAGCAA GGCCGCGCTT TGCCCCCGGG 
GTCACAATTG AGGAGGATAA CTGCTGCGGC TGTAACGCCA TCGCCATAAA ACGCCACTTC 
TTAGACGAGA ACATGAACAT GGTGGACATT GTGTATACTT CATGCCATGA TGCTGTTTAT 
GAAACACCAT TCTTTGTTGC AGTTGATCAT GATAAGAAGA AAGTGGTGAT TAGCATTCGA 
GGGACACTCT CTCCCAAGGA TGCCTTGACA GATCTGACAG GAGATGCGGA ACGGTTGCCA 
GTGGAAGGGC ATCATGGCAC CTGGTTGGGA CACAAGGGAA TGGTTCTGTC AGCTGAATAC 
ATTAAAAAGA AGCTGGAGCA AGAGATGGTT CTTTCCCAAG CATTTGGCCG TGACCTGGCA 
CGTGGGACCA AACACTATGA ACTGATTGTG GTTGGCCATT CGCTTGGAGC TGGAACAGCT 
GCCATCTTGT CCTTCCTGTT ACGCCCGCAG TATCCTTCCC TGAAGTGCTT TGCATATTCC 
CCACCTGGTG GCTTGTTAAG TGAGGATGCG ATGGAATATT CTAAGGAATT TGTTACCTCT 
GTGGTGCTTG GAAAGGATCT AGTTCCAAGA ATCGGCCTCT CGCAGCTGGA GGGTTTTCGC 
AGGCACTTGC TTGATGTTCT TCAGAGAAGC AACAAGCCTA AGTGGCGCAT CATACTTGGC 
GGAACCAAAT GCATACCTAA ATCAGAACTC CCGGAAGAGT CAGAGGCTAT AACGATACCC 
AGTAATCGCC TATGGACGCA CCCCAGTGAC CTGACCATAG CACTTTCAGC TAGCACACCC 
CTTTACCCAC CGGGGCGTAT CATTCATGTG GTTCACAACC ATCCAGTTGA GCAATGCTGT 
TTCTGGAATC AGGAAGAACC CACCTACTTT GCCATCTGGG GTGATAATAA GGCATTTGAG 
GAGGTCATAA TCTCTCCAGC CATGTTGCAT GAACATCTCC CACACGTGGT AATGGAAGGA 
CTGAACAAGG TCCTAGAGAA TTACAACAAG GGGAAAACCG CGCTGCTCTC CGCTGCCAAG 
GTCATGGTGA GTCCAACGGA AGTGGATCTG AACCCAGAGC TGATTTTCCA ACAGCAACCT 
CACCCGCCTC CCCCAATCCT GGAGGTAATT CCTGCCATCA TGCCTGGAGC CCACAACAGG 
AGGAACAGCA GCACCAAGAG TAAGCAGTCA GACATCAGCC TGGAAGGTTT CTATGACTCT 
CGCCTTCTGT CACCAATTCT AAAAGACCCA GTAGAGCTCC TTTTGATGAG CACAAAAGAT 
TGCCTGGCTG TATCTCTCCA AGATCGCAGG GCACCTCTCG CCACCATGGA GAGCCTCTCA 
GATAATGAAT CTGTTTACAG CTTTGATTCA CGGCGCTCTT CTGGTTTCCG CAGTATACGA 
GGCTCACCGA GCCTGAGAGC AGTGTTGGAG CGCGATGAGA GTCATTGTTT CTTCATTGAC 
CCCACGATCC CTGAGGAAAA CCCCTCTTTG AGCTCACGGA CAGAGCTTCT AGGAGCAGAT 
GCGCTCTCAA AGCATTCGCA GGAGAGCCAG CCGACTGAGG TTGCTCACAA TAGTGGCAGA 
TCTTCACCAG GTGACCTGCC TCCTTGGGAT GAGGAGGCCT TAGCAGGAGT AGAGAGCAGC 
AGGGCTCTCA CACCAGCTAA AGAAGAATTG GCACTGCAGA ATGGGCGCTT GAATGAGGTA 
CCTAGTCCCC AGGTGCTGGA GTTTGCAGAA TTTATAGACA GCCTTTTTAA TCTGGACAAC 
AAAAGTGGTT CTCTGCAGGA TCTTTACCAT GTGATGGTTT CAGATGGTAC AGACCGTGAT 
ATACCTAAGT CAGTTAGTGA CCAGGAAATC CTACTTCGGG CAAAGTTTGA ACCTAATCTG 
GTGCCCAAGC CCCCTCGCCT CTTTGCTGGC TCTGCAGATC CATCCTCTGG CATTTCAGTG 
TCCGCCTCCT TCCCTCTCAG CTCCTCTGGG GAGCTCATGG ACTTAACGCC AACGGGTATG 
AGCAGCCAGG AGTGCCTGAC GCCAGACCCC ATCCGAACTC CAACGCCAAC AGGAGCCAGT 
AACGGCAAAA AGGAAGAGTT GCTTATTTCT GCTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913466.1
CDS	141..3239
Translation	MPGIVVFRRRWSVGSDDLVLPGIFLFVLHSTWFVILSVVLFGLTYNPHETCSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLAQYYTSCNDVTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQASSWTRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRKTKYLDLKNAQEMQRYKEVCYYMLFALAAYGWPMYLIRNPTCGICRLAGACSSCCCLCSARPRFAPGVTIEEDNCCGCNAIAIKRHFLDENMNMVDIVYTSCHDAVYETPFFVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLARGTKHYELIVVGHSLGAGTAAILSFLLRPQYPSLKCFAYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLDVLQRSNKPKWRIILGGTKCIPKSELPEESEAITIPSNRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPVEQCCFWNQEEPTYFAIWGDNKAFEEVIISPAMLHEHLPHVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLNPELIFQQQPHPPPPILEVIPAIMPGAHNRRNSSTKSKQSDISLEGFYDSRLLSPILKDPVELLLMSTKDCLAVSLQDRRAPLATMESLSDNESVYSFDSRRSSGFRSIRGSPSLRAVLERDESHCFFIDPTIPEENPSLSSRTELLGADALSKHSQESQPTEVAHNSGRSSPGDLPPWDEEALAGVESSRALTPAKEELALQNGRLNEVPSPQVLEFAEFIDSLFNLDNKSGSLQDLYHVMVSDGTDRDIPKSVSDQEILLRAKFEPNLVPKPPRLFAGSADPSSGISVSASFPLSSSGELMDLTPTGMSSQECLTPDPIRTPTPTGASNGKKEELLISAL

Target ORF information:

RefSeq Version	XM_004913409.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913409.2

ORF Insert Sequence:

ATGCCAGGAA TTGTGGTTTT CCGCCGCCGC TGGTCTGTGG GCAGCGATGA TTTGGTGCTG 
CCCGGAATCT TCCTCTTCGT GCTGCATAGT ACCTGGTTTG TTATCCTGTC TGTGGTTCTC 
TTTGGACTGA CGTATAACCC ACACGAGACA TGCTCCCTCA ACTTGGTAGA TCATGGTCGG 
GGGTACCTGG GCATCCTGCT CAGCTGCATG ATTGCAGAAA TGGCCATTAT CTGGCTGAGC 
ATGAGAGGGG GCATCCTATA CACCGAGCCT CGGGACTCCA TGCAGTATGT GCTCTACGTG 
CGCCTGGCGA TCCTGGTGAT TGAGTTCATA TACGCAATCG TGGGCATTGT CTGGCTGGCT 
CAGTATTACA CGTCGTGCAA TGATGTCACT GCCAAAAATG TCACACTGGG GATGGTGGTG 
TGTAACTGGG TGGTCATACT CAGTGTGTGC ATCACAGTCC TCTGTGTGTT CGATCCAACC 
GGAAGAACTT TCGTGAAGCT GAGGGCAACA AAAAGGAGAC AGAGAAACCT GCGTACATAC 
AACCTGCGGC ACCGACTAGA AGAAGGCCAG GCCAGCAGCT GGACACGGCG CCTGAAGGTG 
TTTCTGTGCT GCACGCGCAC TAAAGACTCC CAGTCTGATG CATACTCTGA GATCGCCTAC 
TTATTTGCTG AGTTTTTCAG AGATCTTGAC ATTGTTCCAT CAGACATCAT TGCTGGACTG 
GTTCTACTGC GCCAGCGTCA AAGGGCAAAA CGCAACGCTG TTCTCGATGA GGCAAACAAT 
GACATATTGG CTTTTCTATC TGGGATGCCC GTCACCAGGA AAACAAAATA CCTAGACCTG 
AAAAATGCAC AAGAGATGCA GCGGTACAAG GAGGTCTGTT ACTACATGCT CTTTGCGCTG 
GCAGCATATG GCTGGCCTAT GTACCTCATT AGGAACCCAA CCTGTGGCAT CTGCCGTTTG 
GCAGGGGCAT GCTCCAGTTG CTGCTGCCTT TGCTCAGCAA GGCCGCGCTT TGCCCCCGGG 
GTCACAATTG AGGAGGATAA CTGCTGCGGC TGTAACGCCA TCGCCATAAA ACGCCACTTC 
TTAGACGAGA ACATGAACAT GGTGGACATT GTGTATACTT CATGCCATGA TGCTGTTTAT 
GAAACACCAT TCTTTGTTGC AGTTGATCAT GATAAGAAGA AAGTGGTGAT TAGCATTCGA 
GGGACACTCT CTCCCAAGGA TGCCTTGACA GATCTGACAG GAGATGCGGA ACGGTTGCCA 
GTGGAAGGGC ATCATGGCAC CTGGTTGGGA CACAAGGGAA TGGTTCTGTC AGCTGAATAC 
ATTAAAAAGA AGCTGGAGCA AGAGATGGTT CTTTCCCAAG CATTTGGCCG TGACCTGGCA 
CGTGGGACCA AACACTATGA ACTGATTGTG GTTGGCCATT CGCTTGGAGC TGGAACAGCT 
GCCATCTTGT CCTTCCTGTT ACGCCCGCAG TATCCTTCCC TGAAGTGCTT TGCATATTCC 
CCACCTGGTG GCTTGTTAAG TGAGGATGCG ATGGAATATT CTAAGGAATT TGTTACCTCT 
GTGGTGCTTG GAAAGGATCT AGTTCCAAGA ATCGGCCTCT CGCAGCTGGA GGGTTTTCGC 
AGGCACTTGC TTGATGTTCT TCAGAGAAGC AACAAGCCTA AGTGGCGCAT CATACTTGGC 
GGAACCAAAT GCATACCTAA ATCAGAACTC CCGGAAGAGT CAGAGGCTAT AACGATACCC 
AGTAATCGCC TATGGACGCA CCCCAGTGAC CTGACCATAG CACTTTCAGC TAGCACACCC 
CTTTACCCAC CGGGGCGTAT CATTCATGTG GTTCACAACC ATCCAGTTGA GCAATGCTGT 
TTCTGGAATC AGGAAGAACC CACCTACTTT GCCATCTGGG GTGATAATAA GGCATTTGAG 
GAGGTCATAA TCTCTCCAGC CATGTTGCAT GAACATCTCC CACACGTGGT AATGGAAGGA 
CTGAACAAGG TCCTAGAGAA TTACAACAAG GGGAAAACCG CGCTGCTCTC CGCTGCCAAG 
GTCATGGTGA GTCCAACGGA AGTGGATCTG AACCCAGAGC TGATTTTCCA ACAGCAACCT 
CACCCGCCTC CCCCAATCCT GGAGGTAATT CCTGCCATCA TGCCTGGAGC CCACAACAGG 
AGGAACAGCA GCACCAAGAG TAAGCAGTCA GACATCAGCC TGGAAGGTTT CTATGACTCT 
CGCCTTCTGT CACCAATTCT AAAAGACCCA GTAGAGCTCC TTTTGATGAG CACAAAAGAT 
TGCCTGGCTG TATCTCTCCA AGATCGCAGG GCACCTCTCG CCACCATGGA GAGCCTCTCA 
GATAATGAAT CTGTTTACAG CTTTGATTCA CGGCGCTCTT CTGGTTTCCG CAGTATACGA 
GGCTCACCGA GCCTGAGAGC AGTGTTGGAG CGCGATGAGA GTCATTGTTT CTTCATTGAC 
CCCACGATCC CTGAGGAAAA CCCCTCTTTG AGCTCACGGA CAGAGCTTCT AGGAGCAGAT 
GCGCTCTCAA AGCATTCGCA GGAGAGCCAG CCGACTGAGG TTGCTCACAA TAGTGGCAGA 
TCTTCACCAG GTGACCTGCC TCCTTGGGAT GAGGAGGCCT TAGCAGGAGT AGAGAGCAGC 
AGGGCTCTCA CACCAGCTAA AGAAGAATTG GCACTGCAGA ATGGGCGCTT GAATGAGGTA 
CCTAGTCCCC AGGTGCTGGA GTTTGCAGAA TTTATAGACA GCCTTTTTAA TCTGGACAAC 
AAAAGTGGTT CTCTGCAGGA TCTTTACCAT GTGATGGTTT CAGATGGTAC AGACCGTGAT 
ATACCTAAGT CAGTTAGTGA CCAGGAAATC CTACTTCGGG CAAAGTTTGA ACCTAATCTG 
GTGCCCAAGC CCCCTCGCCT CTTTGCTGGC TCTGCAGATC CATCCTCTGG CATTTCAGTG 
TCCGCCTCCT TCCCTCTCAG CTCCTCTGGG GAGCTCATGG ACTTAACGCC AACGGGTATG 
AGCAGCCAGG AGTGCCTGAC GCCAGACCCC ATCCGAACTC CAACGCCAAC AGGAGCCAGT 
AACGGCAAAA AGGAAGAGTT GCTTATTTCT GCTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dagla ( XM_004913408.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913465.1 Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X1, mRNA.

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CloneID	OXa08578
Clone ID Related Accession (Same CDS sequence)	XM_004913409.2 , XM_004913408.1
Accession Version	XM_004913408.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGAA TTGTGGTTTT CCGCCGCCGC TGGTCTGTGG GCAGCGATGA TTTGGTGCTG 
CCCGGAATCT TCCTCTTCGT GCTGCATAGT ACCTGGTTTG TTATCCTGTC TGTGGTTCTC 
TTTGGACTGA CGTATAACCC ACACGAGACA TGCTCCCTCA ACTTGGTAGA TCATGGTCGG 
GGGTACCTGG GCATCCTGCT CAGCTGCATG ATTGCAGAAA TGGCCATTAT CTGGCTGAGC 
ATGAGAGGGG GCATCCTATA CACCGAGCCT CGGGACTCCA TGCAGTATGT GCTCTACGTG 
CGCCTGGCGA TCCTGGTGAT TGAGTTCATA TACGCAATCG TGGGCATTGT CTGGCTGGCT 
CAGTATTACA CGTCGTGCAA TGATGTCACT GCCAAAAATG TCACACTGGG GATGGTGGTG 
TGTAACTGGG TGGTCATACT CAGTGTGTGC ATCACAGTCC TCTGTGTGTT CGATCCAACC 
GGAAGAACTT TCGTGAAGCT GAGGGCAACA AAAAGGAGAC AGAGAAACCT GCGTACATAC 
AACCTGCGGC ACCGACTAGA AGAAGGCCAG GCCAGCAGCT GGACACGGCG CCTGAAGGTG 
TTTCTGTGCT GCACGCGCAC TAAAGACTCC CAGTCTGATG CATACTCTGA GATCGCCTAC 
TTATTTGCTG AGTTTTTCAG AGATCTTGAC ATTGTTCCAT CAGACATCAT TGCTGGACTG 
GTTCTACTGC GCCAGCGTCA AAGGGCAAAA CGCAACGCTG TTCTCGATGA GGCAAACAAT 
GACATATTGG CTTTTCTATC TGGGATGCCC GTCACCAGGA AAACAAAATA CCTAGACCTG 
AAAAATGCAC AAGAGATGCA GCGGTACAAG GAGGTCTGTT ACTACATGCT CTTTGCGCTG 
GCAGCATATG GCTGGCCTAT GTACCTCATT AGGAACCCAA CCTGTGGCAT CTGCCGTTTG 
GCAGGGGCAT GCTCCAGTTG CTGCTGCCTT TGCTCAGCAA GGCCGCGCTT TGCCCCCGGG 
GTCACAATTG AGGAGGATAA CTGCTGCGGC TGTAACGCCA TCGCCATAAA ACGCCACTTC 
TTAGACGAGA ACATGAACAT GGTGGACATT GTGTATACTT CATGCCATGA TGCTGTTTAT 
GAAACACCAT TCTTTGTTGC AGTTGATCAT GATAAGAAGA AAGTGGTGAT TAGCATTCGA 
GGGACACTCT CTCCCAAGGA TGCCTTGACA GATCTGACAG GAGATGCGGA ACGGTTGCCA 
GTGGAAGGGC ATCATGGCAC CTGGTTGGGA CACAAGGGAA TGGTTCTGTC AGCTGAATAC 
ATTAAAAAGA AGCTGGAGCA AGAGATGGTT CTTTCCCAAG CATTTGGCCG TGACCTGGCA 
CGTGGGACCA AACACTATGA ACTGATTGTG GTTGGCCATT CGCTTGGAGC TGGAACAGCT 
GCCATCTTGT CCTTCCTGTT ACGCCCGCAG TATCCTTCCC TGAAGTGCTT TGCATATTCC 
CCACCTGGTG GCTTGTTAAG TGAGGATGCG ATGGAATATT CTAAGGAATT TGTTACCTCT 
GTGGTGCTTG GAAAGGATCT AGTTCCAAGA ATCGGCCTCT CGCAGCTGGA GGGTTTTCGC 
AGGCACTTGC TTGATGTTCT TCAGAGAAGC AACAAGCCTA AGTGGCGCAT CATACTTGGC 
GGAACCAAAT GCATACCTAA ATCAGAACTC CCGGAAGAGT CAGAGGCTAT AACGATACCC 
AGTAATCGCC TATGGACGCA CCCCAGTGAC CTGACCATAG CACTTTCAGC TAGCACACCC 
CTTTACCCAC CGGGGCGTAT CATTCATGTG GTTCACAACC ATCCAGTTGA GCAATGCTGT 
TTCTGGAATC AGGAAGAACC CACCTACTTT GCCATCTGGG GTGATAATAA GGCATTTGAG 
GAGGTCATAA TCTCTCCAGC CATGTTGCAT GAACATCTCC CACACGTGGT AATGGAAGGA 
CTGAACAAGG TCCTAGAGAA TTACAACAAG GGGAAAACCG CGCTGCTCTC CGCTGCCAAG 
GTCATGGTGA GTCCAACGGA AGTGGATCTG AACCCAGAGC TGATTTTCCA ACAGCAACCT 
CACCCGCCTC CCCCAATCCT GGAGGTAATT CCTGCCATCA TGCCTGGAGC CCACAACAGG 
AGGAACAGCA GCACCAAGAG TAAGCAGTCA GACATCAGCC TGGAAGGTTT CTATGACTCT 
CGCCTTCTGT CACCAATTCT AAAAGACCCA GTAGAGCTCC TTTTGATGAG CACAAAAGAT 
TGCCTGGCTG TATCTCTCCA AGATCGCAGG GCACCTCTCG CCACCATGGA GAGCCTCTCA 
GATAATGAAT CTGTTTACAG CTTTGATTCA CGGCGCTCTT CTGGTTTCCG CAGTATACGA 
GGCTCACCGA GCCTGAGAGC AGTGTTGGAG CGCGATGAGA GTCATTGTTT CTTCATTGAC 
CCCACGATCC CTGAGGAAAA CCCCTCTTTG AGCTCACGGA CAGAGCTTCT AGGAGCAGAT 
GCGCTCTCAA AGCATTCGCA GGAGAGCCAG CCGACTGAGG TTGCTCACAA TAGTGGCAGA 
TCTTCACCAG GTGACCTGCC TCCTTGGGAT GAGGAGGCCT TAGCAGGAGT AGAGAGCAGC 
AGGGCTCTCA CACCAGCTAA AGAAGAATTG GCACTGCAGA ATGGGCGCTT GAATGAGGTA 
CCTAGTCCCC AGGTGCTGGA GTTTGCAGAA TTTATAGACA GCCTTTTTAA TCTGGACAAC 
AAAAGTGGTT CTCTGCAGGA TCTTTACCAT GTGATGGTTT CAGATGGTAC AGACCGTGAT 
ATACCTAAGT CAGTTAGTGA CCAGGAAATC CTACTTCGGG CAAAGTTTGA ACCTAATCTG 
GTGCCCAAGC CCCCTCGCCT CTTTGCTGGC TCTGCAGATC CATCCTCTGG CATTTCAGTG 
TCCGCCTCCT TCCCTCTCAG CTCCTCTGGG GAGCTCATGG ACTTAACGCC AACGGGTATG 
AGCAGCCAGG AGTGCCTGAC GCCAGACCCC ATCCGAACTC CAACGCCAAC AGGAGCCAGT 
AACGGCAAAA AGGAAGAGTT GCTTATTTCT GCTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913465.1
CDS	1081..4179
Translation	MPGIVVFRRRWSVGSDDLVLPGIFLFVLHSTWFVILSVVLFGLTYNPHETCSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLAQYYTSCNDVTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQASSWTRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRKTKYLDLKNAQEMQRYKEVCYYMLFALAAYGWPMYLIRNPTCGICRLAGACSSCCCLCSARPRFAPGVTIEEDNCCGCNAIAIKRHFLDENMNMVDIVYTSCHDAVYETPFFVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLARGTKHYELIVVGHSLGAGTAAILSFLLRPQYPSLKCFAYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLDVLQRSNKPKWRIILGGTKCIPKSELPEESEAITIPSNRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPVEQCCFWNQEEPTYFAIWGDNKAFEEVIISPAMLHEHLPHVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLNPELIFQQQPHPPPPILEVIPAIMPGAHNRRNSSTKSKQSDISLEGFYDSRLLSPILKDPVELLLMSTKDCLAVSLQDRRAPLATMESLSDNESVYSFDSRRSSGFRSIRGSPSLRAVLERDESHCFFIDPTIPEENPSLSSRTELLGADALSKHSQESQPTEVAHNSGRSSPGDLPPWDEEALAGVESSRALTPAKEELALQNGRLNEVPSPQVLEFAEFIDSLFNLDNKSGSLQDLYHVMVSDGTDRDIPKSVSDQEILLRAKFEPNLVPKPPRLFAGSADPSSGISVSASFPLSSSGELMDLTPTGMSSQECLTPDPIRTPTPTGASNGKKEELLISAL

Target ORF information:

RefSeq Version	XM_004913408.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol lipase, alpha (dagla), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913408.1

ORF Insert Sequence:

ATGCCAGGAA TTGTGGTTTT CCGCCGCCGC TGGTCTGTGG GCAGCGATGA TTTGGTGCTG 
CCCGGAATCT TCCTCTTCGT GCTGCATAGT ACCTGGTTTG TTATCCTGTC TGTGGTTCTC 
TTTGGACTGA CGTATAACCC ACACGAGACA TGCTCCCTCA ACTTGGTAGA TCATGGTCGG 
GGGTACCTGG GCATCCTGCT CAGCTGCATG ATTGCAGAAA TGGCCATTAT CTGGCTGAGC 
ATGAGAGGGG GCATCCTATA CACCGAGCCT CGGGACTCCA TGCAGTATGT GCTCTACGTG 
CGCCTGGCGA TCCTGGTGAT TGAGTTCATA TACGCAATCG TGGGCATTGT CTGGCTGGCT 
CAGTATTACA CGTCGTGCAA TGATGTCACT GCCAAAAATG TCACACTGGG GATGGTGGTG 
TGTAACTGGG TGGTCATACT CAGTGTGTGC ATCACAGTCC TCTGTGTGTT CGATCCAACC 
GGAAGAACTT TCGTGAAGCT GAGGGCAACA AAAAGGAGAC AGAGAAACCT GCGTACATAC 
AACCTGCGGC ACCGACTAGA AGAAGGCCAG GCCAGCAGCT GGACACGGCG CCTGAAGGTG 
TTTCTGTGCT GCACGCGCAC TAAAGACTCC CAGTCTGATG CATACTCTGA GATCGCCTAC 
TTATTTGCTG AGTTTTTCAG AGATCTTGAC ATTGTTCCAT CAGACATCAT TGCTGGACTG 
GTTCTACTGC GCCAGCGTCA AAGGGCAAAA CGCAACGCTG TTCTCGATGA GGCAAACAAT 
GACATATTGG CTTTTCTATC TGGGATGCCC GTCACCAGGA AAACAAAATA CCTAGACCTG 
AAAAATGCAC AAGAGATGCA GCGGTACAAG GAGGTCTGTT ACTACATGCT CTTTGCGCTG 
GCAGCATATG GCTGGCCTAT GTACCTCATT AGGAACCCAA CCTGTGGCAT CTGCCGTTTG 
GCAGGGGCAT GCTCCAGTTG CTGCTGCCTT TGCTCAGCAA GGCCGCGCTT TGCCCCCGGG 
GTCACAATTG AGGAGGATAA CTGCTGCGGC TGTAACGCCA TCGCCATAAA ACGCCACTTC 
TTAGACGAGA ACATGAACAT GGTGGACATT GTGTATACTT CATGCCATGA TGCTGTTTAT 
GAAACACCAT TCTTTGTTGC AGTTGATCAT GATAAGAAGA AAGTGGTGAT TAGCATTCGA 
GGGACACTCT CTCCCAAGGA TGCCTTGACA GATCTGACAG GAGATGCGGA ACGGTTGCCA 
GTGGAAGGGC ATCATGGCAC CTGGTTGGGA CACAAGGGAA TGGTTCTGTC AGCTGAATAC 
ATTAAAAAGA AGCTGGAGCA AGAGATGGTT CTTTCCCAAG CATTTGGCCG TGACCTGGCA 
CGTGGGACCA AACACTATGA ACTGATTGTG GTTGGCCATT CGCTTGGAGC TGGAACAGCT 
GCCATCTTGT CCTTCCTGTT ACGCCCGCAG TATCCTTCCC TGAAGTGCTT TGCATATTCC 
CCACCTGGTG GCTTGTTAAG TGAGGATGCG ATGGAATATT CTAAGGAATT TGTTACCTCT 
GTGGTGCTTG GAAAGGATCT AGTTCCAAGA ATCGGCCTCT CGCAGCTGGA GGGTTTTCGC 
AGGCACTTGC TTGATGTTCT TCAGAGAAGC AACAAGCCTA AGTGGCGCAT CATACTTGGC 
GGAACCAAAT GCATACCTAA ATCAGAACTC CCGGAAGAGT CAGAGGCTAT AACGATACCC 
AGTAATCGCC TATGGACGCA CCCCAGTGAC CTGACCATAG CACTTTCAGC TAGCACACCC 
CTTTACCCAC CGGGGCGTAT CATTCATGTG GTTCACAACC ATCCAGTTGA GCAATGCTGT 
TTCTGGAATC AGGAAGAACC CACCTACTTT GCCATCTGGG GTGATAATAA GGCATTTGAG 
GAGGTCATAA TCTCTCCAGC CATGTTGCAT GAACATCTCC CACACGTGGT AATGGAAGGA 
CTGAACAAGG TCCTAGAGAA TTACAACAAG GGGAAAACCG CGCTGCTCTC CGCTGCCAAG 
GTCATGGTGA GTCCAACGGA AGTGGATCTG AACCCAGAGC TGATTTTCCA ACAGCAACCT 
CACCCGCCTC CCCCAATCCT GGAGGTAATT CCTGCCATCA TGCCTGGAGC CCACAACAGG 
AGGAACAGCA GCACCAAGAG TAAGCAGTCA GACATCAGCC TGGAAGGTTT CTATGACTCT 
CGCCTTCTGT CACCAATTCT AAAAGACCCA GTAGAGCTCC TTTTGATGAG CACAAAAGAT 
TGCCTGGCTG TATCTCTCCA AGATCGCAGG GCACCTCTCG CCACCATGGA GAGCCTCTCA 
GATAATGAAT CTGTTTACAG CTTTGATTCA CGGCGCTCTT CTGGTTTCCG CAGTATACGA 
GGCTCACCGA GCCTGAGAGC AGTGTTGGAG CGCGATGAGA GTCATTGTTT CTTCATTGAC 
CCCACGATCC CTGAGGAAAA CCCCTCTTTG AGCTCACGGA CAGAGCTTCT AGGAGCAGAT 
GCGCTCTCAA AGCATTCGCA GGAGAGCCAG CCGACTGAGG TTGCTCACAA TAGTGGCAGA 
TCTTCACCAG GTGACCTGCC TCCTTGGGAT GAGGAGGCCT TAGCAGGAGT AGAGAGCAGC 
AGGGCTCTCA CACCAGCTAA AGAAGAATTG GCACTGCAGA ATGGGCGCTT GAATGAGGTA 
CCTAGTCCCC AGGTGCTGGA GTTTGCAGAA TTTATAGACA GCCTTTTTAA TCTGGACAAC 
AAAAGTGGTT CTCTGCAGGA TCTTTACCAT GTGATGGTTT CAGATGGTAC AGACCGTGAT 
ATACCTAAGT CAGTTAGTGA CCAGGAAATC CTACTTCGGG CAAAGTTTGA ACCTAATCTG 
GTGCCCAAGC CCCCTCGCCT CTTTGCTGGC TCTGCAGATC CATCCTCTGG CATTTCAGTG 
TCCGCCTCCT TCCCTCTCAG CTCCTCTGGG GAGCTCATGG ACTTAACGCC AACGGGTATG 
AGCAGCCAGG AGTGCCTGAC GCCAGACCCC ATCCGAACTC CAACGCCAAC AGGAGCCAGT 
AACGGCAAAA AGGAAGAGTT GCTTATTTCT GCTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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dagla cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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