DAGLA cDNA ORF clone, Bos taurus(cattle)

The following DAGLA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAGLA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa231998 NM_001192583.3
Latest version!
Bos taurus diacylglycerol lipase alpha (DAGLA), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa231998 XM_005226931.4
Latest version!
Bos taurus diacylglycerol lipase alpha (DAGLA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa231998 XM_005226930.4
Latest version!
Bos taurus diacylglycerol lipase alpha (DAGLA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa34664 NM_001192583.2
Latest version!
Bos taurus diacylglycerol lipase alpha (DAGLA), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa37613 NM_001192583.1
Latest version!
Bos taurus diacylglycerol lipase, alpha (DAGLA), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa34664 XM_005226931.3
Latest version!
Bos taurus diacylglycerol lipase, alpha (DAGLA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa34664 XM_005226930.3
Latest version!
Bos taurus diacylglycerol lipase, alpha (DAGLA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa231998
    Clone ID Related Accession (Same CDS sequence) NM_001192583.3 , XM_005226931.4 , XM_005226930.4
    Accession Version NM_001192583.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3126bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product sn1-specific diacylglycerol lipase alpha
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000029.1. On May 30, 2018 this sequence version replaced NM_001192583.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR6764281.8000.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
    CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT TTGGCTCACC
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC
    TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG
    TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC
    CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC
    GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG
    GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG
    GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC
    ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC
    GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC
    CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC
    CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG
    GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG
    AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC
    CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT
    GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC
    AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC
    CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC
    GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG
    ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG
    GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC
    ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC
    AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG
    CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG
    CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC
    ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC
    TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC
    CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG
    CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC
    CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG
    GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC
    CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC
    AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC
    CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA
    GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC
    CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG
    GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT
    TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA
    CGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179512.1
    CDS1..3126
    Translation

    Target ORF information:

    RefSeq Version NM_001192583.3
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol lipase alpha (DAGLA), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192583.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
    CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT TTGGCTCACC
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC
    TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG
    TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC
    CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC
    GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG
    GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG
    GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC
    ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC
    GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC
    CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC
    CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG
    GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG
    AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC
    CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT
    GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC
    AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC
    CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC
    GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG
    ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG
    GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC
    ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC
    AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG
    CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG
    CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC
    ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC
    TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC
    CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG
    CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC
    CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG
    GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC
    CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC
    AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC
    CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA
    GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC
    CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG
    GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT
    TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA
    CGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231998
    Clone ID Related Accession (Same CDS sequence) NM_001192583.3 , XM_005226931.4 , XM_005226930.4
    Accession Version XM_005226931.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3126bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sn1-specific diacylglycerol lipase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226931.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
    CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT TTGGCTCACC
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC
    TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG
    TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC
    CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC
    GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG
    GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG
    GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC
    ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC
    GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC
    CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC
    CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG
    GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG
    AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC
    CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT
    GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC
    AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC
    CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC
    GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG
    ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG
    GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC
    ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC
    AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG
    CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG
    CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC
    ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC
    TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC
    CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG
    CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC
    CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG
    GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC
    CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC
    AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC
    CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA
    GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC
    CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG
    GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT
    TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA
    CGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226988.1
    CDS179..3304
    Translation

    Target ORF information:

    RefSeq Version XM_005226931.4
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol lipase alpha (DAGLA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226931.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
    CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT TTGGCTCACC
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC
    TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG
    TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC
    CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC
    GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG
    GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG
    GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC
    ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC
    GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC
    CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC
    CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG
    GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG
    AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC
    CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT
    GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC
    AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC
    CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC
    GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG
    ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG
    GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC
    ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC
    AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG
    CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG
    CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC
    ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC
    TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC
    CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG
    CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC
    CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG
    GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC
    CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC
    AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC
    CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA
    GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC
    CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG
    GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT
    TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA
    CGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231998
    Clone ID Related Accession (Same CDS sequence) NM_001192583.3 , XM_005226931.4 , XM_005226930.4
    Accession Version XM_005226930.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3126bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sn1-specific diacylglycerol lipase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226930.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
    CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT TTGGCTCACC
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC
    TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG
    TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC
    CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC
    GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG
    GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG
    GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC
    ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC
    GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC
    CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC
    CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG
    GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG
    AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC
    CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT
    GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC
    AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC
    CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC
    GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG
    ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG
    GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC
    ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC
    AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG
    CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG
    CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC
    ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC
    TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC
    CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG
    CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC
    CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG
    GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC
    CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC
    AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC
    CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA
    GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC
    CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG
    GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT
    TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA
    CGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226987.1
    CDS2752..5877
    Translation

    Target ORF information:

    RefSeq Version XM_005226930.4
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol lipase alpha (DAGLA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226930.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
    CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT TTGGCTCACC
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC
    TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG
    TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC
    CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC
    GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG
    GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG
    GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC
    ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC
    GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC
    CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC
    CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG
    GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG
    AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC
    CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT
    GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC
    AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC
    CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC
    GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG
    ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG
    GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC
    ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC
    AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG
    CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG
    CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC
    ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC
    TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC
    CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG
    CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC
    CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG
    GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC
    CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC
    AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC
    CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA
    GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC
    CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG
    GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT
    TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA
    CGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34664
    Clone ID Related Accession (Same CDS sequence) XM_005226931.3 , XM_005226930.3 , NM_001192583.2
    Accession Version NM_001192583.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3126bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product sn1-specific diacylglycerol lipase alpha
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02063470.1. On Jun 30, 2017 this sequence version replaced NM_001192583.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092, SAMN02822093 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
    CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT CTGGCTCACC
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC
    TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG
    TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC
    CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC
    GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG
    GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG
    GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC
    ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC
    GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC
    CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC
    CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG
    GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG
    AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC
    CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT
    GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC
    AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC
    CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC
    GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG
    ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG
    GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC
    ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC
    AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG
    CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG
    CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC
    ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC
    TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC
    CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG
    CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC
    CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG
    GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC
    CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC
    AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC
    CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA
    GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC
    CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG
    GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT
    TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA
    CGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179512.1
    CDS1..3126
    Translation

    Target ORF information:

    RefSeq Version NM_001192583.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol lipase alpha (DAGLA), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192583.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
    CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT CTGGCTCACC
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC
    TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG
    TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC
    CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC
    GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG
    GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG
    GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC
    ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC
    GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC
    CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC
    CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG
    GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG
    AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC
    CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT
    GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC
    AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC
    CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC
    GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG
    ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG
    GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC
    ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC
    AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG
    CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG
    CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC
    ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC
    TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC
    CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG
    CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC
    CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG
    GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC
    CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC
    AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC
    CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA
    GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC
    CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG
    GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT
    TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA
    CGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37613
    Clone ID Related Accession (Same CDS sequence) NM_001192583.1
    Accession Version NM_001192583.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3126bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product sn1-specific diacylglycerol lipase alpha
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03045766.1. On Jul 17, 2010 this sequence version replaced XM_601967.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01093727, SAMN01093728 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
    CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT CTGGCTCACC
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC
    TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG
    TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC
    CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC
    GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG
    GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG
    GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC
    ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC
    GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGATGCGGT CTATGAAACC
    CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC
    CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG
    GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG
    AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC
    CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT
    GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC
    AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC
    CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC
    GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG
    ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG
    GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC
    ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC
    AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG
    CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG
    CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC
    ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC
    TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC
    CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG
    CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC
    CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG
    GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC
    CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC
    AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC
    CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA
    GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC
    CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG
    GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT
    TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA
    CGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179512.1
    CDS1..3126
    Translation

    Target ORF information:

    RefSeq Version NM_001192583.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus diacylglycerol lipase, alpha (DAGLA), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192583.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
    CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT CTGGCTCACC
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC
    TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG
    TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC
    CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC
    GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG
    GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG
    GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC
    ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC
    GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGATGCGGT CTATGAAACC
    CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC
    CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG
    GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG
    AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC
    CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT
    GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC
    AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC
    CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC
    GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG
    ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG
    GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC
    ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC
    AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG
    CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG
    CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC
    ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC
    TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC
    CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG
    CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC
    CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG
    GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC
    CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC
    AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC
    CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA
    GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC
    CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG
    GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT
    TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA
    CGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34664
    Clone ID Related Accession (Same CDS sequence) XM_005226931.3 , XM_005226930.3 , NM_001192583.2
    Accession Version XM_005226931.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3126bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sn1-specific diacylglycerol lipase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104646.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226931.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
    CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT CTGGCTCACC
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC
    TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG
    TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC
    CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC
    GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG
    GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG
    GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC
    ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC
    GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC
    CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC
    CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG
    GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG
    AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC
    CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT
    GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC
    AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC
    CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC
    GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG
    ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG
    GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC
    ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC
    AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG
    CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG
    CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC
    ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC
    TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC
    CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG
    CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC
    CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG
    GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC
    CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC
    AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC
    CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA
    GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC
    CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG
    GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT
    TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA
    CGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226988.1
    CDS186..3311
    Translation

    Target ORF information:

    RefSeq Version XM_005226931.3
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol lipase, alpha (DAGLA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226931.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
    CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT CTGGCTCACC
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC
    TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG
    TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC
    CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC
    GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG
    GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG
    GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC
    ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC
    GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC
    CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC
    CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG
    GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG
    AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC
    CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT
    GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC
    AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC
    CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC
    GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG
    ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG
    GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC
    ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC
    AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG
    CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG
    CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC
    ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC
    TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC
    CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG
    CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC
    CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG
    GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC
    CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC
    AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC
    CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA
    GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC
    CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG
    GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT
    TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA
    CGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34664
    Clone ID Related Accession (Same CDS sequence) XM_005226931.3 , XM_005226930.3 , NM_001192583.2
    Accession Version XM_005226930.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3126bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sn1-specific diacylglycerol lipase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104646.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226930.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
    CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT CTGGCTCACC
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC
    TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG
    TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC
    CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC
    GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG
    GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG
    GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC
    ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC
    GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC
    CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC
    CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG
    GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG
    AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC
    CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT
    GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC
    AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC
    CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC
    GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG
    ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG
    GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC
    ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC
    AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG
    CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG
    CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC
    ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC
    TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC
    CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG
    CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC
    CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG
    GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC
    CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC
    AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC
    CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA
    GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC
    CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG
    GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT
    TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA
    CGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226987.1
    CDS5032..8157
    Translation

    Target ORF information:

    RefSeq Version XM_005226930.3
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol lipase, alpha (DAGLA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226930.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
    CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC
    TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC
    GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC
    ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG
    CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT CTGGCTCACC
    CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC
    TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG
    GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC
    AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG
    TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC
    CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG
    GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC
    GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC
    AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG
    GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG
    GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC
    ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC
    GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC
    CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC
    CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG
    GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG
    AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA
    ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC
    CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT
    GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT
    CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG
    CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC
    AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG
    CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC
    CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC
    GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG
    ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC
    AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG
    GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC
    ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC
    AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG
    CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG
    CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC
    ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC
    TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC
    CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG
    CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC
    CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG
    GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC
    CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC
    AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC
    CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA
    GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC
    CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG
    GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT
    TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA
    CGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.