Txnrd2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Txnrd2
Entrez Gene ID	26462
Full Name	thioredoxin reductase 2
Synonyms	AA118373,ESTM573010,TGR,Tr3,Trxr2,Trxrd2
General protein information	Preferred Names thioredoxin reductase 2 Names thioredoxin reductase 2, mitochondrial TR beta thioredoxin reductase TR3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 A3\|16 11.41 cM
Summary	The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013711.3	NP_038739.2	thioredoxin reductase 2, mitochondrial isoform 1 precursor
NM_001353143.1	NP_001340072.1	thioredoxin reductase 2, mitochondrial isoform 2 precursor

Pathways from BioSystems

Homologies

	TXNRD2	NP_001152971.1
Drosophila melanogaster (fruit fly)	Trxr-2	NP_524216.1
Drosophila melanogaster (fruit fly)	Trxr-1	NP_727252.1
Anopheles gambiae (African malaria mosquito)	TRXR	XP_003437030.1
Caenorhabditis elegans (roundworm)	trxr-2	NP_498971.1
Homo sapiens (human)	TXNRD2	NP_006431.2
Mus musculus (house mouse)	Txnrd2	NP_038739.2
Rattus norvegicus (Norway rat)	Txnrd2	NP_072106.1
Pan troglodytes (chimpanzee)	TXNRD2	XP_001166615.2
Xenopus tropicalis (tropical clawed frog)	txnrd2	NP_001120487.1
Canis lupus familiaris (dog)	TXNRD2	XP_005636641.1
Bos taurus (cattle)	TXNRD2	NP_777051.1
Gallus gallus (chicken)	TXNRD2	NP_001116163.1

Gene Ontology
Bibliography

Related articles in PubMed

Endothelial Dysfunction, and A Prothrombotic, Proinflammatory Phenotype Is Caused by Loss of Mitochondrial Thioredoxin Reductase in Endothelium.
Kirsch J, Schneider H, Pagel JI, Rehberg M, Singer M, Hellfritsch J, Chillo O, Schubert KM, Qiu J, Pogoda K, Kameritsch P, Uhl B, Pircher J, Deindl E, M?ller S, Kirchner T, Pohl U, Conrad M, Beck H
Arteriosclerosis, thrombosis, and vascular biology36(9)1891-9(2016 Sep)

Heart-Specific Knockout of the Mitochondrial Thioredoxin Reductase (Txnrd2) Induces Metabolic and Contractile Dysfunction in the Aging Myocardium.
Kiermayer C, Northrup E, Schrewe A, Walch A, de Angelis MH, Schoensiegel F, Zischka H, Prehn C, Adamski J, Bekeredjian R, Ivandic B, Kupatt C, Brielmeier M
Journal of the American Heart Association4(7)(2015 Jul)

Genomic organization and identification of a novel alternative splicing variant of mouse mitochondrial thioredoxin reductase (TrxR2) gene.
Miranda-Vizuete A, Spyrou G
Molecules and cells13(3)488-92(2002 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Maintenance of mitochondrial ROS via Txnrd2 in endothelial cells is necessary for an intact vascular homeostasis and remodeling. Txnrd2 plays a vitally important role in balancing mitochondrial ROS production in the endothelium.
Title: Endothelial Dysfunction, and A Prothrombotic, Proinflammatory Phenotype Is Caused by Loss of Mitochondrial Thioredoxin Reductase in Endothelium.

Suggest role for Txnrd2 in sustaining heart function during aging and suggest that Txnrd2 may be a modifier of heart failure.
Title: Heart-Specific Knockout of the Mitochondrial Thioredoxin Reductase (Txnrd2) Induces Metabolic and Contractile Dysfunction in the Aging Myocardium.

Regulatory link was discovered between mitochondrial Txnrd and the JNK-PHD2-Hif-1alpha axis, which highlights how the loss of Txnrd2 and the resulting altered mitochondrial redox balance impairs tumor growth as well as tumor-related angiogenesis.
Title: Knockout of mitochondrial thioredoxin reductase stabilizes prolyl hydroxylase 2 and inhibits tumor growth and tumor-derived angiogenesis.

Data suggest TXNRD1 and TXRNRD2 function at the top of a redox pyramid that governs the oxidation state of peroxiredoxins and other protein factors, thereby dictating a hierarchy of phenotypic responses to oxidative insults.
Title: Resolution of oxidative stress by thioredoxin reductase: Cysteine versus selenocysteine.

Data indicate that mammalian thioredoxin reductase (H-TrxR) reduces hypothiocyanous acid (HOSCN).
Title: Selective metabolism of hypothiocyanous acid by mammalian thioredoxin reductase promotes lung innate immunity and antioxidant defense.

The following Txnrd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Txnrd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu83897	NM_001353143.1 Latest version!	Mus musculus thioredoxin reductase 2 (Txnrd2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu15209	NM_013711.3 Latest version!	Mus musculus thioredoxin reductase 2 (Txnrd2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Txnrd2 ( NM_001353143.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001340072.1 Mus musculus thioredoxin reductase 2 (Txnrd2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu83897
Clone ID Related Accession (Same CDS sequence)	NM_001353143.1
Accession Version	NM_001353143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	thioredoxin reductase 2, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB027566.1, AF412308.1 and BF465261.1. Transcript Variant: This variant (2) lacks an in-frame coding exon in the 3' region compared to variant 1. The resulting shorter isoform (2) is missing an internal protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF412308.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta NMD candidate :: translation inferred from conservation protein contains selenocysteine :: PMID: 10721726 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGA TGGTGGCGGC GATGGTGGCG GCGCTGCGTG GACCCAGCAG GCGCTTCCGG 
CCGCGGACAC GGGCTCTGAC ACGCGGGACA AGGGGCGCGG CGAGTGCAGC GGGAGGGCAG 
CAGAGCTTTG ATCTCTTGGT GATCGGTGGG GGATCCGGTG GCCTAGCTTG TGCCAAGGAA 
GCTGCTCAGC TGGGAAAGAA GGTGGCTGTG GCTGACTATG TGGAACCTTC TCCCCGAGGC 
ACCAAGTGGG GCCTTGGTGG CACCTGTGTC AACGTGGGTT GCATACCCAA GAAGCTGATG 
CATCAGGCTG CACTGCTGGG GGGCATGATC AGAGATGCTC ACCACTATGG CTGGGAGGTG 
GCCCAGCCTG TCCAACACAA CTGGAAGACA ATGGCAGAAG CCGTGCAAAA CCATGTGAAA 
TCCTTGAACT GGGGTCATCG CGTCCAACTG CAGGACAGGA AAGTCAAGTA CTTTAACATC 
AAAGCCAGCT TTGTGGATGA GCACACAGTT CGCGGTGTGG ACAAAGGCGG GAAGGCGACT 
CTGCTTTCAG CTGAGCACAT TGTCATTGCT ACAGGAGGAC GGCCAAGGTA CCCCACACAA 
GTCAAAGGAG CCCTGGAATA TGGAATCACA AGTGACGACA TCTTCTGGCT GAAGGAGTCC 
CCTGGGAAAA CGTTGGTGGT TGGAGCCAGC TATGTGGCCC TAGAGTGTGC TGGCTTCCTC 
ACTGGAATTG GACTGGATAC CACTGTCATG ATGCGCAGCA TCCCTCTCCG AGGCTTTGAC 
CAGCAAATGT CATCTTTGGT CACAGAGCAC ATGGAGTCTC ATGGCACCCA GTTCCTGAAA 
GGCTGTGTCC CCTCCCACAT CAAAAAACTC CCAACTAACC AGCTGCAGGT CACTTGGGAG 
GATCATGCTT CTGGCAAGGA AGACACAGGC ACCTTTGACA CTGTCCTGTG GGCCATAGGG 
CGAGTTCCAG AAACCAGGAC TTTGAATCTG GAGAAGGCTG GCATCAGTAC CAACCCTAAG 
AATCAGAAGA TTATTGTGGA TGCCCAGGAG GCTACCTCTG TTCCCCACAT CTATGCCATT 
GGAGATGTTG CTGAGGGGCG GCCTGAGCTG ACGCCCACAG CTATCAAGGC AGGAAAGCTT 
CTGGCTCAGC GGCTCTTTGG GAAATCCTCA ACCTTAATGG ATTACAGCAA TGTTTACCAT 
GCATATTATA AGCCCCTAGA GTTCACGGTG GCGGATAGGG ATGCATCACA GTGCTACATA 
AAGATGGTAT GCATGAGGGA GCCCCCACAA CTGGTGCTGG GCCTGCACTT CCTTGGCCCC 
AACGCTGGAG AAGTCACCCA AGGATTTGCT CTTGGGATCA AGTGTGGGGC TTCATATGCA 
CAGGTGATGC AGACAGTAGG GATCCATCCC ACCTGCTCTG AGGAGGTGGT CAAGCTGCAC 
ATCTCCAAGC GCTCCGGCCT GGAGCCTACT GTGACTGGTT GCTGAGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340072.1
CDS	29..1519
Translation	MAAMVAAMVAALRGPSRRFRPRTRALTRGTRGAASAAGGQQSFDLLVIGGGSGGLACAKEAAQLGKKVAVADYVEPSPRGTKWGLGGTCVNVGCIPKKLMHQAALLGGMIRDAHHYGWEVAQPVQHNWKTMAEAVQNHVKSLNWGHRVQLQDRKVKYFNIKASFVDEHTVRGVDKGGKATLLSAEHIVIATGGRPRYPTQVKGALEYGITSDDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTVMMRSIPLRGFDQQMSSLVTEHMESHGTQFLKGCVPSHIKKLPTNQLQVTWEDHASGKEDTGTFDTVLWAIGRVPETRTLNLEKAGISTNPKNQKIIVDAQEATSVPHIYAIGDVAEGRPELTPTAIKAGKLLAQRLFGKSSTLMDYSNVYHAYYKPLEFTVADRDASQCYIKMVCMREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMQTVGIHPTCSEEVVKLHISKRSGLEPTVTGC*G

Target ORF information:

RefSeq Version	NM_001353143.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thioredoxin reductase 2 (Txnrd2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353143.1

ORF Insert Sequence:

ATGGCGGCGA TGGTGGCGGC GATGGTGGCG GCGCTGCGTG GACCCAGCAG GCGCTTCCGG 
CCGCGGACAC GGGCTCTGAC ACGCGGGACA AGGGGCGCGG CGAGTGCAGC GGGAGGGCAG 
CAGAGCTTTG ATCTCTTGGT GATCGGTGGG GGATCCGGTG GCCTAGCTTG TGCCAAGGAA 
GCTGCTCAGC TGGGAAAGAA GGTGGCTGTG GCTGACTATG TGGAACCTTC TCCCCGAGGC 
ACCAAGTGGG GCCTTGGTGG CACCTGTGTC AACGTGGGTT GCATACCCAA GAAGCTGATG 
CATCAGGCTG CACTGCTGGG GGGCATGATC AGAGATGCTC ACCACTATGG CTGGGAGGTG 
GCCCAGCCTG TCCAACACAA CTGGAAGACA ATGGCAGAAG CCGTGCAAAA CCATGTGAAA 
TCCTTGAACT GGGGTCATCG CGTCCAACTG CAGGACAGGA AAGTCAAGTA CTTTAACATC 
AAAGCCAGCT TTGTGGATGA GCACACAGTT CGCGGTGTGG ACAAAGGCGG GAAGGCGACT 
CTGCTTTCAG CTGAGCACAT TGTCATTGCT ACAGGAGGAC GGCCAAGGTA CCCCACACAA 
GTCAAAGGAG CCCTGGAATA TGGAATCACA AGTGACGACA TCTTCTGGCT GAAGGAGTCC 
CCTGGGAAAA CGTTGGTGGT TGGAGCCAGC TATGTGGCCC TAGAGTGTGC TGGCTTCCTC 
ACTGGAATTG GACTGGATAC CACTGTCATG ATGCGCAGCA TCCCTCTCCG AGGCTTTGAC 
CAGCAAATGT CATCTTTGGT CACAGAGCAC ATGGAGTCTC ATGGCACCCA GTTCCTGAAA 
GGCTGTGTCC CCTCCCACAT CAAAAAACTC CCAACTAACC AGCTGCAGGT CACTTGGGAG 
GATCATGCTT CTGGCAAGGA AGACACAGGC ACCTTTGACA CTGTCCTGTG GGCCATAGGG 
CGAGTTCCAG AAACCAGGAC TTTGAATCTG GAGAAGGCTG GCATCAGTAC CAACCCTAAG 
AATCAGAAGA TTATTGTGGA TGCCCAGGAG GCTACCTCTG TTCCCCACAT CTATGCCATT 
GGAGATGTTG CTGAGGGGCG GCCTGAGCTG ACGCCCACAG CTATCAAGGC AGGAAAGCTT 
CTGGCTCAGC GGCTCTTTGG GAAATCCTCA ACCTTAATGG ATTACAGCAA TGTTTACCAT 
GCATATTATA AGCCCCTAGA GTTCACGGTG GCGGATAGGG ATGCATCACA GTGCTACATA 
AAGATGGTAT GCATGAGGGA GCCCCCACAA CTGGTGCTGG GCCTGCACTT CCTTGGCCCC 
AACGCTGGAG AAGTCACCCA AGGATTTGCT CTTGGGATCA AGTGTGGGGC TTCATATGCA 
CAGGTGATGC AGACAGTAGG GATCCATCCC ACCTGCTCTG AGGAGGTGGT CAAGCTGCAC 
ATCTCCAAGC GCTCCGGCCT GGAGCCTACT GTGACTGGTT GCTGAGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Txnrd2 ( NM_013711.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038739.2 Mus musculus thioredoxin reductase 2 (Txnrd2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15209
Clone ID Related Accession (Same CDS sequence)	NM_013711.3
Accession Version	NM_013711.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	thioredoxin reductase 2, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB027566.1 and BF465261.1. On Apr 17, 2007 this sequence version replaced NM_013711.2. Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF136399.1, BC029344.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta NMD candidate :: translation inferred from conservation protein contains selenocysteine :: PMID: 10721726 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGA TGGTGGCGGC GATGGTGGCG GCGCTGCGTG GACCCAGCAG GCGCTTCCGG 
CCGCGGACAC GGGCTCTGAC ACGCGGGACA AGGGGCGCGG CGAGTGCAGC GGGAGGGCAG 
CAGAGCTTTG ATCTCTTGGT GATCGGTGGG GGATCCGGTG GCCTAGCTTG TGCCAAGGAA 
GCTGCTCAGC TGGGAAAGAA GGTGGCTGTG GCTGACTATG TGGAACCTTC TCCCCGAGGC 
ACCAAGTGGG GCCTTGGTGG CACCTGTGTC AACGTGGGTT GCATACCCAA GAAGCTGATG 
CATCAGGCTG CACTGCTGGG GGGCATGATC AGAGATGCTC ACCACTATGG CTGGGAGGTG 
GCCCAGCCTG TCCAACACAA CTGGAAGACA ATGGCAGAAG CCGTGCAAAA CCATGTGAAA 
TCCTTGAACT GGGGTCATCG CGTCCAACTG CAGGACAGGA AAGTCAAGTA CTTTAACATC 
AAAGCCAGCT TTGTGGATGA GCACACAGTT CGCGGTGTGG ACAAAGGCGG GAAGGCGACT 
CTGCTTTCAG CTGAGCACAT TGTCATTGCT ACAGGAGGAC GGCCAAGGTA CCCCACACAA 
GTCAAAGGAG CCCTGGAATA TGGAATCACA AGTGACGACA TCTTCTGGCT GAAGGAGTCC 
CCTGGGAAAA CGTTGGTGGT TGGAGCCAGC TATGTGGCCC TAGAGTGTGC TGGCTTCCTC 
ACTGGAATTG GACTGGATAC CACTGTCATG ATGCGCAGCA TCCCTCTCCG AGGCTTTGAC 
CAGCAAATGT CATCTTTGGT CACAGAGCAC ATGGAGTCTC ATGGCACCCA GTTCCTGAAA 
GGCTGTGTCC CCTCCCACAT CAAAAAACTC CCAACTAACC AGCTGCAGGT CACTTGGGAG 
GATCATGCTT CTGGCAAGGA AGACACAGGC ACCTTTGACA CTGTCCTGTG GGCCATAGGG 
CGAGTTCCAG AAACCAGGAC TTTGAATCTG GAGAAGGCTG GCATCAGTAC CAACCCTAAG 
AATCAGAAGA TTATTGTGGA TGCCCAGGAG GCTACCTCTG TTCCCCACAT CTATGCCATT 
GGAGATGTTG CTGAGGGGCG GCCTGAGCTG ACGCCCACAG CTATCAAGGC AGGAAAGCTT 
CTGGCTCAGC GGCTCTTTGG GAAATCCTCA ACCTTAATGG ATTACAGCAA TGTTCCCACA 
ACTGTCTTTA CACCACTGGA GTATGGCTGT GTGGGGCTGT CTGAGGAGGA GGCTGTGGCT 
CTCCATGGCC AGGAGCATGT AGAGGTTTAC CATGCATATT ATAAGCCCCT AGAGTTCACG 
GTGGCGGATA GGGATGCATC ACAGTGCTAC ATAAAGATGG TATGCATGAG GGAGCCCCCA 
CAACTGGTGC TGGGCCTGCA CTTCCTTGGC CCCAACGCTG GAGAAGTCAC CCAAGGATTT 
GCTCTTGGGA TCAAGTGTGG GGCTTCATAT GCACAGGTGA TGCAGACAGT AGGGATCCAT 
CCCACCTGCT CTGAGGAGGT GGTCAAGCTG CACATCTCCA AGCGCTCCGG CCTGGAGCCT 
ACTGTGACTG GTTGCTGAGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038739.2
CDS	29..1612
Translation	MAAMVAAMVAALRGPSRRFRPRTRALTRGTRGAASAAGGQQSFDLLVIGGGSGGLACAKEAAQLGKKVAVADYVEPSPRGTKWGLGGTCVNVGCIPKKLMHQAALLGGMIRDAHHYGWEVAQPVQHNWKTMAEAVQNHVKSLNWGHRVQLQDRKVKYFNIKASFVDEHTVRGVDKGGKATLLSAEHIVIATGGRPRYPTQVKGALEYGITSDDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTVMMRSIPLRGFDQQMSSLVTEHMESHGTQFLKGCVPSHIKKLPTNQLQVTWEDHASGKEDTGTFDTVLWAIGRVPETRTLNLEKAGISTNPKNQKIIVDAQEATSVPHIYAIGDVAEGRPELTPTAIKAGKLLAQRLFGKSSTLMDYSNVPTTVFTPLEYGCVGLSEEEAVALHGQEHVEVYHAYYKPLEFTVADRDASQCYIKMVCMREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMQTVGIHPTCSEEVVKLHISKRSGLEPTVTGC*G

Target ORF information:

RefSeq Version	NM_013711.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thioredoxin reductase 2 (Txnrd2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013711.3

ORF Insert Sequence:

ATGGCGGCGA TGGTGGCGGC GATGGTGGCG GCGCTGCGTG GACCCAGCAG GCGCTTCCGG 
CCGCGGACAC GGGCTCTGAC ACGCGGGACA AGGGGCGCGG CGAGTGCAGC GGGAGGGCAG 
CAGAGCTTTG ATCTCTTGGT GATCGGTGGG GGATCCGGTG GCCTAGCTTG TGCCAAGGAA 
GCTGCTCAGC TGGGAAAGAA GGTGGCTGTG GCTGACTATG TGGAACCTTC TCCCCGAGGC 
ACCAAGTGGG GCCTTGGTGG CACCTGTGTC AACGTGGGTT GCATACCCAA GAAGCTGATG 
CATCAGGCTG CACTGCTGGG GGGCATGATC AGAGATGCTC ACCACTATGG CTGGGAGGTG 
GCCCAGCCTG TCCAACACAA CTGGAAGACA ATGGCAGAAG CCGTGCAAAA CCATGTGAAA 
TCCTTGAACT GGGGTCATCG CGTCCAACTG CAGGACAGGA AAGTCAAGTA CTTTAACATC 
AAAGCCAGCT TTGTGGATGA GCACACAGTT CGCGGTGTGG ACAAAGGCGG GAAGGCGACT 
CTGCTTTCAG CTGAGCACAT TGTCATTGCT ACAGGAGGAC GGCCAAGGTA CCCCACACAA 
GTCAAAGGAG CCCTGGAATA TGGAATCACA AGTGACGACA TCTTCTGGCT GAAGGAGTCC 
CCTGGGAAAA CGTTGGTGGT TGGAGCCAGC TATGTGGCCC TAGAGTGTGC TGGCTTCCTC 
ACTGGAATTG GACTGGATAC CACTGTCATG ATGCGCAGCA TCCCTCTCCG AGGCTTTGAC 
CAGCAAATGT CATCTTTGGT CACAGAGCAC ATGGAGTCTC ATGGCACCCA GTTCCTGAAA 
GGCTGTGTCC CCTCCCACAT CAAAAAACTC CCAACTAACC AGCTGCAGGT CACTTGGGAG 
GATCATGCTT CTGGCAAGGA AGACACAGGC ACCTTTGACA CTGTCCTGTG GGCCATAGGG 
CGAGTTCCAG AAACCAGGAC TTTGAATCTG GAGAAGGCTG GCATCAGTAC CAACCCTAAG 
AATCAGAAGA TTATTGTGGA TGCCCAGGAG GCTACCTCTG TTCCCCACAT CTATGCCATT 
GGAGATGTTG CTGAGGGGCG GCCTGAGCTG ACGCCCACAG CTATCAAGGC AGGAAAGCTT 
CTGGCTCAGC GGCTCTTTGG GAAATCCTCA ACCTTAATGG ATTACAGCAA TGTTCCCACA 
ACTGTCTTTA CACCACTGGA GTATGGCTGT GTGGGGCTGT CTGAGGAGGA GGCTGTGGCT 
CTCCATGGCC AGGAGCATGT AGAGGTTTAC CATGCATATT ATAAGCCCCT AGAGTTCACG 
GTGGCGGATA GGGATGCATC ACAGTGCTAC ATAAAGATGG TATGCATGAG GGAGCCCCCA 
CAACTGGTGC TGGGCCTGCA CTTCCTTGGC CCCAACGCTG GAGAAGTCAC CCAAGGATTT 
GCTCTTGGGA TCAAGTGTGG GGCTTCATAT GCACAGGTGA TGCAGACAGT AGGGATCCAT 
CCCACCTGCT CTGAGGAGGT GGTCAAGCTG CACATCTCCA AGCGCTCCGG CCTGGAGCCT 
ACTGTGACTG GTTGCTGAGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Endothelial Dysfunction, and A Prothrombotic, Proinflammatory Phenotype Is Caused by Loss of Mitochondrial Thioredoxin Reductase in Endothelium.
Arteriosclerosis, thrombosis, and vascular biology36(9)1891-9(2016 Sep)
Kirsch J,Schneider H,Pagel JI,Rehberg M,Singer M,Hellfritsch J,Chillo O,Schubert KM,Qiu J,Pogoda K,Kameritsch P,Uhl B,Pircher J,Deindl E,M?ller S,Kirchner T,Pohl U,Conrad M,Beck H

Heart-Specific Knockout of the Mitochondrial Thioredoxin Reductase (Txnrd2) Induces Metabolic and Contractile Dysfunction in the Aging Myocardium.
Journal of the American Heart Association4(7)(2015 Jul)
Kiermayer C,Northrup E,Schrewe A,Walch A,de Angelis MH,Schoensiegel F,Zischka H,Prehn C,Adamski J,Bekeredjian R,Ivandic B,Kupatt C,Brielmeier M

Genomic organization and identification of a novel alternative splicing variant of mouse mitochondrial thioredoxin reductase (TrxR2) gene.
Molecules and cells13(3)488-92(2002 Jun)
Miranda-Vizuete A,Spyrou G

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Txnrd2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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