Trxr-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trxr-1
Entrez Gene ID	31760
Full Name	Thioredoxin reductase-1
Synonyms	2151,CG2151,DTR,Dm-TrxR,DmTR,DmTrx,DmTrxR,DmTrxR-1,Dmel\CG2151,GR,Gr,TH,TRR,Trx,TrxR,TrxR-1,Trxr,Trxr1,anon-WO03040301.185,anon-WO03040301.187,dTrxR,dmtrxr-1,gr,l(1)G0154,l(1)G0379,l(1)G0477,l(1)G0481,trxr-1
General protein information	Preferred Names Thioredoxin reductase-1 Names CG2151 gene product from transcript CG2151-RA CG2151-PA CG2151-PB CG2151-PC Trxr-1-PA Trxr-1-PB Trxr-1-PC disulfide reductase glutathione reductase thioredoxin reductase thioredoxin reductase-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7D22-7E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_167150.2	NP_727252.1	thioredoxin reductase-1, isoform C
NM_078527.3	NP_511082.2	thioredoxin reductase-1, isoform A
NM_167149.2	NP_727251.1	thioredoxin reductase-1, isoform B

Pathways from BioSystems

Homologies

	TXNRD2	NP_001152971.1
Drosophila melanogaster (fruit fly)	Trxr-2	NP_524216.1
Drosophila melanogaster (fruit fly)	Trxr-1	NP_727252.1
Anopheles gambiae (African malaria mosquito)	TRXR	XP_003437030.1
Caenorhabditis elegans (roundworm)	trxr-2	NP_498971.1
Homo sapiens (human)	TXNRD2	NP_006431.2
Mus musculus (house mouse)	Txnrd2	NP_038739.2
Rattus norvegicus (Norway rat)	Txnrd2	NP_072106.1
Pan troglodytes (chimpanzee)	TXNRD2	XP_001166615.2
Xenopus tropicalis (tropical clawed frog)	txnrd2	NP_001120487.1
Canis lupus familiaris (dog)	TXNRD2	XP_005636641.1
Bos taurus (cattle)	TXNRD2	NP_777051.1
Gallus gallus (chicken)	TXNRD2	NP_001116163.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of mitochondrial TXNRD2 in Drosophila melanogaster extended median lifespan in female flies with a small lifespan extension in males; in contrast, overexpression of the cytosolic form, TXNRD1, did not produce a lifespan extension.
Title: Mitochondrial thioredoxin reductase 2 is elevated in long-lived primate as well as rodent species and extends fly mean lifespan.

Molecular orbital calculations suggested that the C-terminal hexapeptide Pro-Ala-Ser-Cys-Cys-Ser-OH functions as a redox center that alleviates the necessity for selenium in Dm-TrxR.
Title: Identification and conformer analysis of a novel redox-active motif, Pro-Ala-Ser-Cys-Cys-Ser, in Drosophila thioredoxin reductase by semiempirical molecular orbital calculation.

the role of Glu-469' in catalysis by DmTrxR
Title: Function of Glu-469' in the acid-base catalysis of thioredoxin reductase from Drosophila melanogaster.

rates of steps in both the reductive and the oxidative half-reactions are markedly diminished in H464'Q thioredoxin reductase as compared to those of wild-type enzyme, indicating that His-464' is involved in both half-reactions
Title: Acid-base catalysis in the mechanism of thioredoxin reductase from Drosophila melanogaster.

redox potentials provide direct evidence for proposed catalytic mechanism of DmTrxR, & cast new light on essential role of DmTrx system in cycling GSSG/GSH & maintaining intracellular redox homeostasis in D. melanogaster without glutathione reductase.
Title: The relationship of the redox potentials of thioredoxin and thioredoxin reductase from Drosophila melanogaster to the enzymatic mechanism: reduced thioredoxin is the reductant of glutathione in Drosophila.

The following Trxr-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trxr-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01097	NM_078527.3 Latest version!	Drosophila melanogaster thioredoxin reductase-1, transcript variant A (Trxr-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01098	NM_167149.2 Latest version!	Drosophila melanogaster thioredoxin reductase-1, transcript variant B (Trxr-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa01099	NM_167150.2 Latest version!	Drosophila melanogaster thioredoxin reductase-1, transcript variant C (Trxr-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trxr-1 ( NM_078527.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511082.2 Drosophila melanogaster thioredoxin reductase-1, transcript variant A (Trxr-1), mRNA.

Price & Availability↑

CloneID	OFa01097
Clone ID Related Accession (Same CDS sequence)	NM_078527.3
Accession Version	NM_078527.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thioredoxin reductase-1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078527.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCCCG TGCAAGGATC CTACGACTAC GACCTTATTG TGATTGGAGG CGGCTCAGCT 
GGCCTGGCCT GCGCCAAGGA GGCAGTCCTC AATGGAGCCC GTGTGGCCTG TCTGGATTTC 
GTTAAGCCCA CGCCCACTCT GGGCACCAAG TGGGGCGTTG GCGGCACCTG CGTGAACGTG 
GGCTGCATTC CCAAGAAGCT GATGCACCAG GCCTCCCTTC TGGGCGAGGC TGTCCATGAG 
GCGGCCGCCT ACGGCTGGAA CGTGGACGAA AAGATCAAGC CAGACTGGCA CAAGCTGGTG 
CAGTCCGTAC AGAACCACAT CAAGTCCGTC AACTGGGTGA CCCGTGTGGA TCTGCGCGAC 
AAGAAAGTGG AGTACATCAA TGGACTGGGC TCCTTCGTGG ACTCGCACAC ACTGCTGGCC 
AAGCTGAAGA GCGGCGAGCG CACAATCACC GCCCAGACCT TCGTCATTGC CGTTGGCGGC 
CGACCACGTT ATCCGGATAT TCCCGGTGCT GTCGAGTATG GCATCACCAG CGATGATCTG 
TTCAGTTTGG ACCGCGAGCC CGGCAAGACC CTGGTGGTGG GAGCTGGCTA CATTGGCTTG 
GAGTGCGCTG GATTCCTGAA GGGTCTCGGC TACGAGCCCA CTGTGATGGT GCGTTCTATT 
GTGCTGCGTG GCTTCGACCA GCAGATGGCC GAGCTGGTGG CAGCCTCGAT GGAGGAGCGT 
GGCATTCCCT TCCTCCGCAA GACGGTGCCG CTGTCCGTGG AAAAGCAGGA TGATGGCAAG 
CTGCTCGTGA AGTACAAGAA CGTGGAGACC GGCGAGGAGG CCGAGGATGT TTACGACACC 
GTTCTGTGGG CCATCGGCCG CAAGGGTCTG GTGGACGATC TGAACCTGCC CAATGCCGGC 
GTGACTGTGC AGAAGGACAA GATTCCAGTG GACTCCCAGG AGGCTACCAA TGTGGCAAAC 
ATCTACGCTG TCGGCGATAT CATCTATGGC AAGCCAGAGC TGACGCCCGT CGCCGTTTTG 
GCTGGCCGTT TGCTGGCCCG CCGCCTGTAC GGAGGATCTA CCCAGCGCAT GGACTACAAG 
GATGTGGCCA CCACCGTTTT CACGCCCCTG GAGTACGCCT GCGTCGGCCT GAGCGAGGAG 
GATGCCGTCA AGCAGTTCGG AGCCGATGAG ATCGAGGTGT TCCACGGCTA CTACAAGCCC 
ACGGAGTTCT TCATTCCCCA GAAGAGCGTG CGCTACTGCT ACTTGAAGGC TGTGGCCGAG 
CGCCATGGTG ACCAGCGCGT CTATGGACTG CACTATATTG GCCCGGTGGC CGGTGAGGTT 
ATCCAGGGAT TCGCTGCCGC TTTGAAGTCT GGCCTGACTA TTAACACGCT GATCAACACC 
GTGGGCATCC ATCCCACTAC CGCCGAAGAA TTCACCCGGC TGGCCATCAC CAAGCGCTCC 
GGACTGGACC CCACGCCGGC CAGCTGCTGC AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511082.2
CDS	189..1664
Translation	MAPVQGSYDYDLIVIGGGSAGLACAKEAVLNGARVACLDFVKPTPTLGTKWGVGGTCVNVGCIPKKLMHQASLLGEAVHEAAAYGWNVDEKIKPDWHKLVQSVQNHIKSVNWVTRVDLRDKKVEYINGLGSFVDSHTLLAKLKSGERTITAQTFVIAVGGRPRYPDIPGAVEYGITSDDLFSLDREPGKTLVVGAGYIGLECAGFLKGLGYEPTVMVRSIVLRGFDQQMAELVAASMEERGIPFLRKTVPLSVEKQDDGKLLVKYKNVETGEEAEDVYDTVLWAIGRKGLVDDLNLPNAGVTVQKDKIPVDSQEATNVANIYAVGDIIYGKPELTPVAVLAGRLLARRLYGGSTQRMDYKDVATTVFTPLEYACVGLSEEDAVKQFGADEIEVFHGYYKPTEFFIPQKSVRYCYLKAVAERHGDQRVYGLHYIGPVAGEVIQGFAAALKSGLTINTLINTVGIHPTTAEEFTRLAITKRSGLDPTPASCCS

Target ORF information:

RefSeq Version	NM_078527.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thioredoxin reductase-1, transcript variant A (Trxr-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078527.3

ORF Insert Sequence:

ATGGCGCCCG TGCAAGGATC CTACGACTAC GACCTTATTG TGATTGGAGG CGGCTCAGCT 
GGCCTGGCCT GCGCCAAGGA GGCAGTCCTC AATGGAGCCC GTGTGGCCTG TCTGGATTTC 
GTTAAGCCCA CGCCCACTCT GGGCACCAAG TGGGGCGTTG GCGGCACCTG CGTGAACGTG 
GGCTGCATTC CCAAGAAGCT GATGCACCAG GCCTCCCTTC TGGGCGAGGC TGTCCATGAG 
GCGGCCGCCT ACGGCTGGAA CGTGGACGAA AAGATCAAGC CAGACTGGCA CAAGCTGGTG 
CAGTCCGTAC AGAACCACAT CAAGTCCGTC AACTGGGTGA CCCGTGTGGA TCTGCGCGAC 
AAGAAAGTGG AGTACATCAA TGGACTGGGC TCCTTCGTGG ACTCGCACAC ACTGCTGGCC 
AAGCTGAAGA GCGGCGAGCG CACAATCACC GCCCAGACCT TCGTCATTGC CGTTGGCGGC 
CGACCACGTT ATCCGGATAT TCCCGGTGCT GTCGAGTATG GCATCACCAG CGATGATCTG 
TTCAGTTTGG ACCGCGAGCC CGGCAAGACC CTGGTGGTGG GAGCTGGCTA CATTGGCTTG 
GAGTGCGCTG GATTCCTGAA GGGTCTCGGC TACGAGCCCA CTGTGATGGT GCGTTCTATT 
GTGCTGCGTG GCTTCGACCA GCAGATGGCC GAGCTGGTGG CAGCCTCGAT GGAGGAGCGT 
GGCATTCCCT TCCTCCGCAA GACGGTGCCG CTGTCCGTGG AAAAGCAGGA TGATGGCAAG 
CTGCTCGTGA AGTACAAGAA CGTGGAGACC GGCGAGGAGG CCGAGGATGT TTACGACACC 
GTTCTGTGGG CCATCGGCCG CAAGGGTCTG GTGGACGATC TGAACCTGCC CAATGCCGGC 
GTGACTGTGC AGAAGGACAA GATTCCAGTG GACTCCCAGG AGGCTACCAA TGTGGCAAAC 
ATCTACGCTG TCGGCGATAT CATCTATGGC AAGCCAGAGC TGACGCCCGT CGCCGTTTTG 
GCTGGCCGTT TGCTGGCCCG CCGCCTGTAC GGAGGATCTA CCCAGCGCAT GGACTACAAG 
GATGTGGCCA CCACCGTTTT CACGCCCCTG GAGTACGCCT GCGTCGGCCT GAGCGAGGAG 
GATGCCGTCA AGCAGTTCGG AGCCGATGAG ATCGAGGTGT TCCACGGCTA CTACAAGCCC 
ACGGAGTTCT TCATTCCCCA GAAGAGCGTG CGCTACTGCT ACTTGAAGGC TGTGGCCGAG 
CGCCATGGTG ACCAGCGCGT CTATGGACTG CACTATATTG GCCCGGTGGC CGGTGAGGTT 
ATCCAGGGAT TCGCTGCCGC TTTGAAGTCT GGCCTGACTA TTAACACGCT GATCAACACC 
GTGGGCATCC ATCCCACTAC CGCCGAAGAA TTCACCCGGC TGGCCATCAC CAAGCGCTCC 
GGACTGGACC CCACGCCGGC CAGCTGCTGC AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trxr-1 ( NM_167149.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727251.1 Drosophila melanogaster thioredoxin reductase-1, transcript variant B (Trxr-1), mRNA.

Price & Availability↑

CloneID	OFa01098
Clone ID Related Accession (Same CDS sequence)	NM_167149.2
Accession Version	NM_167149.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thioredoxin reductase-1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167149.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTTGT GCAATTCGAG ATTCTCCGTT ACGTTCGTGC GGCAGTGCTC GACGATTTTA 
ACGTCTCCTT CGGCTGGCAT TATACAAAAC AGAGGCTCAC TGACAACAAA GGTTCCCCAT 
TGGATTTCCA GTAGTCTCAG CTGTGCCCAT CACACGTTTC AGCGAACTAT GAACTTGACG 
GGACAGCGAG GATCACGCGA CAGTACTGGA GCTACCGGTG GGAATGCTCC AGCCGGATCC 
GGTGCCGGCG CACCACCACC CTTCCAGCAT CCACATTGCG ACAGGGCGGC CATGTACGCG 
CAACCGGTGC GAAAGATGAG CACCAAAGGA GGATCCTACG ACTACGACCT TATTGTGATT 
GGAGGCGGCT CAGCTGGCCT GGCCTGCGCC AAGGAGGCAG TCCTCAATGG AGCCCGTGTG 
GCCTGTCTGG ATTTCGTTAA GCCCACGCCC ACTCTGGGCA CCAAGTGGGG CGTTGGCGGC 
ACCTGCGTGA ACGTGGGCTG CATTCCCAAG AAGCTGATGC ACCAGGCCTC CCTTCTGGGC 
GAGGCTGTCC ATGAGGCGGC CGCCTACGGC TGGAACGTGG ACGAAAAGAT CAAGCCAGAC 
TGGCACAAGC TGGTGCAGTC CGTACAGAAC CACATCAAGT CCGTCAACTG GGTGACCCGT 
GTGGATCTGC GCGACAAGAA AGTGGAGTAC ATCAATGGAC TGGGCTCCTT CGTGGACTCG 
CACACACTGC TGGCCAAGCT GAAGAGCGGC GAGCGCACAA TCACCGCCCA GACCTTCGTC 
ATTGCCGTTG GCGGCCGACC ACGTTATCCG GATATTCCCG GTGCTGTCGA GTATGGCATC 
ACCAGCGATG ATCTGTTCAG TTTGGACCGC GAGCCCGGCA AGACCCTGGT GGTGGGAGCT 
GGCTACATTG GCTTGGAGTG CGCTGGATTC CTGAAGGGTC TCGGCTACGA GCCCACTGTG 
ATGGTGCGTT CTATTGTGCT GCGTGGCTTC GACCAGCAGA TGGCCGAGCT GGTGGCAGCC 
TCGATGGAGG AGCGTGGCAT TCCCTTCCTC CGCAAGACGG TGCCGCTGTC CGTGGAAAAG 
CAGGATGATG GCAAGCTGCT CGTGAAGTAC AAGAACGTGG AGACCGGCGA GGAGGCCGAG 
GATGTTTACG ACACCGTTCT GTGGGCCATC GGCCGCAAGG GTCTGGTGGA CGATCTGAAC 
CTGCCCAATG CCGGCGTGAC TGTGCAGAAG GACAAGATTC CAGTGGACTC CCAGGAGGCT 
ACCAATGTGG CAAACATCTA CGCTGTCGGC GATATCATCT ATGGCAAGCC AGAGCTGACG 
CCCGTCGCCG TTTTGGCTGG CCGTTTGCTG GCCCGCCGCC TGTACGGAGG ATCTACCCAG 
CGCATGGACT ACAAGGATGT GGCCACCACC GTTTTCACGC CCCTGGAGTA CGCCTGCGTC 
GGCCTGAGCG AGGAGGATGC CGTCAAGCAG TTCGGAGCCG ATGAGATCGA GGTGTTCCAC 
GGCTACTACA AGCCCACGGA GTTCTTCATT CCCCAGAAGA GCGTGCGCTA CTGCTACTTG 
AAGGCTGTGG CCGAGCGCCA TGGTGACCAG CGCGTCTATG GACTGCACTA TATTGGCCCG 
GTGGCCGGTG AGGTTATCCA GGGATTCGCT GCCGCTTTGA AGTCTGGCCT GACTATTAAC 
ACGCTGATCA ACACCGTGGG CATCCATCCC ACTACCGCCG AAGAATTCAC CCGGCTGGCC 
ATCACCAAGC GCTCCGGACT GGACCCCACG CCGGCCAGCT GCTGCAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727251.1
CDS	148..1938
Translation	MNLCNSRFSVTFVRQCSTILTSPSAGIIQNRGSLTTKVPHWISSSLSCAHHTFQRTMNLTGQRGSRDSTGATGGNAPAGSGAGAPPPFQHPHCDRAAMYAQPVRKMSTKGGSYDYDLIVIGGGSAGLACAKEAVLNGARVACLDFVKPTPTLGTKWGVGGTCVNVGCIPKKLMHQASLLGEAVHEAAAYGWNVDEKIKPDWHKLVQSVQNHIKSVNWVTRVDLRDKKVEYINGLGSFVDSHTLLAKLKSGERTITAQTFVIAVGGRPRYPDIPGAVEYGITSDDLFSLDREPGKTLVVGAGYIGLECAGFLKGLGYEPTVMVRSIVLRGFDQQMAELVAASMEERGIPFLRKTVPLSVEKQDDGKLLVKYKNVETGEEAEDVYDTVLWAIGRKGLVDDLNLPNAGVTVQKDKIPVDSQEATNVANIYAVGDIIYGKPELTPVAVLAGRLLARRLYGGSTQRMDYKDVATTVFTPLEYACVGLSEEDAVKQFGADEIEVFHGYYKPTEFFIPQKSVRYCYLKAVAERHGDQRVYGLHYIGPVAGEVIQGFAAALKSGLTINTLINTVGIHPTTAEEFTRLAITKRSGLDPTPASCCS

Target ORF information:

RefSeq Version	NM_167149.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thioredoxin reductase-1, transcript variant B (Trxr-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167149.2

ORF Insert Sequence:

ATGAACTTGT GCAATTCGAG ATTCTCCGTT ACGTTCGTGC GGCAGTGCTC GACGATTTTA 
ACGTCTCCTT CGGCTGGCAT TATACAAAAC AGAGGCTCAC TGACAACAAA GGTTCCCCAT 
TGGATTTCCA GTAGTCTCAG CTGTGCCCAT CACACGTTTC AGCGAACTAT GAACTTGACG 
GGACAGCGAG GATCACGCGA CAGTACTGGA GCTACCGGTG GGAATGCTCC AGCCGGATCC 
GGTGCCGGCG CACCACCACC CTTCCAGCAT CCACATTGCG ACAGGGCGGC CATGTACGCG 
CAACCGGTGC GAAAGATGAG CACCAAAGGA GGATCCTACG ACTACGACCT TATTGTGATT 
GGAGGCGGCT CAGCTGGCCT GGCCTGCGCC AAGGAGGCAG TCCTCAATGG AGCCCGTGTG 
GCCTGTCTGG ATTTCGTTAA GCCCACGCCC ACTCTGGGCA CCAAGTGGGG CGTTGGCGGC 
ACCTGCGTGA ACGTGGGCTG CATTCCCAAG AAGCTGATGC ACCAGGCCTC CCTTCTGGGC 
GAGGCTGTCC ATGAGGCGGC CGCCTACGGC TGGAACGTGG ACGAAAAGAT CAAGCCAGAC 
TGGCACAAGC TGGTGCAGTC CGTACAGAAC CACATCAAGT CCGTCAACTG GGTGACCCGT 
GTGGATCTGC GCGACAAGAA AGTGGAGTAC ATCAATGGAC TGGGCTCCTT CGTGGACTCG 
CACACACTGC TGGCCAAGCT GAAGAGCGGC GAGCGCACAA TCACCGCCCA GACCTTCGTC 
ATTGCCGTTG GCGGCCGACC ACGTTATCCG GATATTCCCG GTGCTGTCGA GTATGGCATC 
ACCAGCGATG ATCTGTTCAG TTTGGACCGC GAGCCCGGCA AGACCCTGGT GGTGGGAGCT 
GGCTACATTG GCTTGGAGTG CGCTGGATTC CTGAAGGGTC TCGGCTACGA GCCCACTGTG 
ATGGTGCGTT CTATTGTGCT GCGTGGCTTC GACCAGCAGA TGGCCGAGCT GGTGGCAGCC 
TCGATGGAGG AGCGTGGCAT TCCCTTCCTC CGCAAGACGG TGCCGCTGTC CGTGGAAAAG 
CAGGATGATG GCAAGCTGCT CGTGAAGTAC AAGAACGTGG AGACCGGCGA GGAGGCCGAG 
GATGTTTACG ACACCGTTCT GTGGGCCATC GGCCGCAAGG GTCTGGTGGA CGATCTGAAC 
CTGCCCAATG CCGGCGTGAC TGTGCAGAAG GACAAGATTC CAGTGGACTC CCAGGAGGCT 
ACCAATGTGG CAAACATCTA CGCTGTCGGC GATATCATCT ATGGCAAGCC AGAGCTGACG 
CCCGTCGCCG TTTTGGCTGG CCGTTTGCTG GCCCGCCGCC TGTACGGAGG ATCTACCCAG 
CGCATGGACT ACAAGGATGT GGCCACCACC GTTTTCACGC CCCTGGAGTA CGCCTGCGTC 
GGCCTGAGCG AGGAGGATGC CGTCAAGCAG TTCGGAGCCG ATGAGATCGA GGTGTTCCAC 
GGCTACTACA AGCCCACGGA GTTCTTCATT CCCCAGAAGA GCGTGCGCTA CTGCTACTTG 
AAGGCTGTGG CCGAGCGCCA TGGTGACCAG CGCGTCTATG GACTGCACTA TATTGGCCCG 
GTGGCCGGTG AGGTTATCCA GGGATTCGCT GCCGCTTTGA AGTCTGGCCT GACTATTAAC 
ACGCTGATCA ACACCGTGGG CATCCATCCC ACTACCGCCG AAGAATTCAC CCGGCTGGCC 
ATCACCAAGC GCTCCGGACT GGACCCCACG CCGGCCAGCT GCTGCAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trxr-1 ( NM_167150.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727252.1 Drosophila melanogaster thioredoxin reductase-1, transcript variant C (Trxr-1), mRNA.

Price & Availability↑

CloneID	OFa01099
Clone ID Related Accession (Same CDS sequence)	NM_167150.2
Accession Version	NM_167150.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thioredoxin reductase-1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167150.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGAAGT ACATGATTTG CGCCATCGTT GTGGGCGCCA AAAAGAGCAC ATCCTCTAAG 
TATAATGGAT CCTACGACTA CGACCTTATT GTGATTGGAG GCGGCTCAGC TGGCCTGGCC 
TGCGCCAAGG AGGCAGTCCT CAATGGAGCC CGTGTGGCCT GTCTGGATTT CGTTAAGCCC 
ACGCCCACTC TGGGCACCAA GTGGGGCGTT GGCGGCACCT GCGTGAACGT GGGCTGCATT 
CCCAAGAAGC TGATGCACCA GGCCTCCCTT CTGGGCGAGG CTGTCCATGA GGCGGCCGCC 
TACGGCTGGA ACGTGGACGA AAAGATCAAG CCAGACTGGC ACAAGCTGGT GCAGTCCGTA 
CAGAACCACA TCAAGTCCGT CAACTGGGTG ACCCGTGTGG ATCTGCGCGA CAAGAAAGTG 
GAGTACATCA ATGGACTGGG CTCCTTCGTG GACTCGCACA CACTGCTGGC CAAGCTGAAG 
AGCGGCGAGC GCACAATCAC CGCCCAGACC TTCGTCATTG CCGTTGGCGG CCGACCACGT 
TATCCGGATA TTCCCGGTGC TGTCGAGTAT GGCATCACCA GCGATGATCT GTTCAGTTTG 
GACCGCGAGC CCGGCAAGAC CCTGGTGGTG GGAGCTGGCT ACATTGGCTT GGAGTGCGCT 
GGATTCCTGA AGGGTCTCGG CTACGAGCCC ACTGTGATGG TGCGTTCTAT TGTGCTGCGT 
GGCTTCGACC AGCAGATGGC CGAGCTGGTG GCAGCCTCGA TGGAGGAGCG TGGCATTCCC 
TTCCTCCGCA AGACGGTGCC GCTGTCCGTG GAAAAGCAGG ATGATGGCAA GCTGCTCGTG 
AAGTACAAGA ACGTGGAGAC CGGCGAGGAG GCCGAGGATG TTTACGACAC CGTTCTGTGG 
GCCATCGGCC GCAAGGGTCT GGTGGACGAT CTGAACCTGC CCAATGCCGG CGTGACTGTG 
CAGAAGGACA AGATTCCAGT GGACTCCCAG GAGGCTACCA ATGTGGCAAA CATCTACGCT 
GTCGGCGATA TCATCTATGG CAAGCCAGAG CTGACGCCCG TCGCCGTTTT GGCTGGCCGT 
TTGCTGGCCC GCCGCCTGTA CGGAGGATCT ACCCAGCGCA TGGACTACAA GGATGTGGCC 
ACCACCGTTT TCACGCCCCT GGAGTACGCC TGCGTCGGCC TGAGCGAGGA GGATGCCGTC 
AAGCAGTTCG GAGCCGATGA GATCGAGGTG TTCCACGGCT ACTACAAGCC CACGGAGTTC 
TTCATTCCCC AGAAGAGCGT GCGCTACTGC TACTTGAAGG CTGTGGCCGA GCGCCATGGT 
GACCAGCGCG TCTATGGACT GCACTATATT GGCCCGGTGG CCGGTGAGGT TATCCAGGGA 
TTCGCTGCCG CTTTGAAGTC TGGCCTGACT ATTAACACGC TGATCAACAC CGTGGGCATC 
CATCCCACTA CCGCCGAAGA ATTCACCCGG CTGGCCATCA CCAAGCGCTC CGGACTGGAC 
CCCACGCCGG CCAGCTGCTG CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727252.1
CDS	243..1769
Translation	MLKYMICAIVVGAKKSTSSKYNGSYDYDLIVIGGGSAGLACAKEAVLNGARVACLDFVKPTPTLGTKWGVGGTCVNVGCIPKKLMHQASLLGEAVHEAAAYGWNVDEKIKPDWHKLVQSVQNHIKSVNWVTRVDLRDKKVEYINGLGSFVDSHTLLAKLKSGERTITAQTFVIAVGGRPRYPDIPGAVEYGITSDDLFSLDREPGKTLVVGAGYIGLECAGFLKGLGYEPTVMVRSIVLRGFDQQMAELVAASMEERGIPFLRKTVPLSVEKQDDGKLLVKYKNVETGEEAEDVYDTVLWAIGRKGLVDDLNLPNAGVTVQKDKIPVDSQEATNVANIYAVGDIIYGKPELTPVAVLAGRLLARRLYGGSTQRMDYKDVATTVFTPLEYACVGLSEEDAVKQFGADEIEVFHGYYKPTEFFIPQKSVRYCYLKAVAERHGDQRVYGLHYIGPVAGEVIQGFAAALKSGLTINTLINTVGIHPTTAEEFTRLAITKRSGLDPTPASCCS

Target ORF information:

RefSeq Version	NM_167150.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thioredoxin reductase-1, transcript variant C (Trxr-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167150.2

ORF Insert Sequence:

ATGCTGAAGT ACATGATTTG CGCCATCGTT GTGGGCGCCA AAAAGAGCAC ATCCTCTAAG 
TATAATGGAT CCTACGACTA CGACCTTATT GTGATTGGAG GCGGCTCAGC TGGCCTGGCC 
TGCGCCAAGG AGGCAGTCCT CAATGGAGCC CGTGTGGCCT GTCTGGATTT CGTTAAGCCC 
ACGCCCACTC TGGGCACCAA GTGGGGCGTT GGCGGCACCT GCGTGAACGT GGGCTGCATT 
CCCAAGAAGC TGATGCACCA GGCCTCCCTT CTGGGCGAGG CTGTCCATGA GGCGGCCGCC 
TACGGCTGGA ACGTGGACGA AAAGATCAAG CCAGACTGGC ACAAGCTGGT GCAGTCCGTA 
CAGAACCACA TCAAGTCCGT CAACTGGGTG ACCCGTGTGG ATCTGCGCGA CAAGAAAGTG 
GAGTACATCA ATGGACTGGG CTCCTTCGTG GACTCGCACA CACTGCTGGC CAAGCTGAAG 
AGCGGCGAGC GCACAATCAC CGCCCAGACC TTCGTCATTG CCGTTGGCGG CCGACCACGT 
TATCCGGATA TTCCCGGTGC TGTCGAGTAT GGCATCACCA GCGATGATCT GTTCAGTTTG 
GACCGCGAGC CCGGCAAGAC CCTGGTGGTG GGAGCTGGCT ACATTGGCTT GGAGTGCGCT 
GGATTCCTGA AGGGTCTCGG CTACGAGCCC ACTGTGATGG TGCGTTCTAT TGTGCTGCGT 
GGCTTCGACC AGCAGATGGC CGAGCTGGTG GCAGCCTCGA TGGAGGAGCG TGGCATTCCC 
TTCCTCCGCA AGACGGTGCC GCTGTCCGTG GAAAAGCAGG ATGATGGCAA GCTGCTCGTG 
AAGTACAAGA ACGTGGAGAC CGGCGAGGAG GCCGAGGATG TTTACGACAC CGTTCTGTGG 
GCCATCGGCC GCAAGGGTCT GGTGGACGAT CTGAACCTGC CCAATGCCGG CGTGACTGTG 
CAGAAGGACA AGATTCCAGT GGACTCCCAG GAGGCTACCA ATGTGGCAAA CATCTACGCT 
GTCGGCGATA TCATCTATGG CAAGCCAGAG CTGACGCCCG TCGCCGTTTT GGCTGGCCGT 
TTGCTGGCCC GCCGCCTGTA CGGAGGATCT ACCCAGCGCA TGGACTACAA GGATGTGGCC 
ACCACCGTTT TCACGCCCCT GGAGTACGCC TGCGTCGGCC TGAGCGAGGA GGATGCCGTC 
AAGCAGTTCG GAGCCGATGA GATCGAGGTG TTCCACGGCT ACTACAAGCC CACGGAGTTC 
TTCATTCCCC AGAAGAGCGT GCGCTACTGC TACTTGAAGG CTGTGGCCGA GCGCCATGGT 
GACCAGCGCG TCTATGGACT GCACTATATT GGCCCGGTGG CCGGTGAGGT TATCCAGGGA 
TTCGCTGCCG CTTTGAAGTC TGGCCTGACT ATTAACACGC TGATCAACAC CGTGGGCATC 
CATCCCACTA CCGCCGAAGA ATTCACCCGG CTGGCCATCA CCAAGCGCTC CGGACTGGAC 
CCCACGCCGG CCAGCTGCTG CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Trxr-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報