Chd2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chd2
Entrez Gene ID	244059
Full Name	chromodomain helicase DNA binding protein 2
Synonyms	2810013C04Rik,2810040A01Rik,5630401D06Rik,AI851092,BC029703,CHD-2
General protein information	Preferred Names chromodomain helicase DNA binding protein 2 Names chromodomain-helicase-DNA-binding protein 2 ATP-dependent helicase CHD2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001081345.2	NP_001074814.2	chromodomain-helicase-DNA-binding protein 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CHD2	XP_413879.3
Danio rerio (zebrafish)	chd2	XP_005159227.1
Oryza sativa (rice)	Os07g0660200	NP_001060530.1
Canis lupus familiaris (dog)	CHD2	XP_005618381.1
Mus musculus (house mouse)	Chd2	NP_001074814.2
Caenorhabditis elegans (roundworm)	chd-1	NP_491994.2
	CHD2	XP_002808499.1
Rattus norvegicus (Norway rat)	Chd2	NP_001100993.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008698	XP_314800.4
Homo sapiens (human)	CHD2	NP_001262.3
Drosophila melanogaster (fruit fly)	Chd1	NP_477197.1
Xenopus tropicalis (tropical clawed frog)	chd2	XP_002933353.1
Pan troglodytes (chimpanzee)	CHD2	XP_001170676.2
Arabidopsis thaliana (thale cress)	CHR5	NP_178970.3

Gene Ontology
Bibliography

Related articles in PubMed

Chd2 regulates chromatin for proper gene expression toward differentiation in mouse embryonic stem cells.
Semba Y, Harada A, Maehara K, Oki S, Meno C, Ueda J, Yamagata K, Suzuki A, Onimaru M, Nogami J, Okada S, Akashi K, Ohkawa Y
Nucleic acids research45(15)8758-8772(2017 Sep)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Chd2 is essential in preventing suppressive chromatin formation for developmentally regulated genes and determines subsequent effects on developmental processes in the undifferentiated state.
Title: Chd2 regulates chromatin for proper gene expression toward differentiation in mouse embryonic stem cells.

inactivation of CHD2 during neurogenesis might contribute to abnormal neurodevelopment
Title: CHD2 is Required for Embryonic Neurogenesis in the Developing Cerebral Cortex.

The data indicated that MyoD determines cell fate and facilitates differentiation- dependent gene expression through Chd2-dependent deposition of H3.3 at myogenic loci prior to differentiation.
Title: Chd2 interacts with H3.3 to determine myogenic cell fate.

Study present evidence that the Chd2 mutant cells are defective in their ability to repair DNA damage induced by ionizing and ultraviolet radiation.
Title: Chromodomain helicase DNA-binding protein 2 affects the repair of X-ray and UV-induced DNA damage.

Chd2-mutant mice present with glomerulopathy, proteinuria, and significantly impaired kidney function.
Title: A mutation in the mouse Chd2 chromatin remodeling enzyme results in a complex renal phenotype.

The following Chd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02175	NM_001081345.2 Latest version!	Mus musculus chromodomain helicase DNA binding protein 2 (Chd2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1343.30 $1919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chd2 ( NM_001081345.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074814.2 Mus musculus chromodomain helicase DNA binding protein 2 (Chd2), mRNA.

Price & Availability↑

CloneID	OMu02175
Clone ID Related Accession (Same CDS sequence)	NM_001081345.2
Accession Version	NM_001081345.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC099699.16 and AC154883.13. On Jun 6, 2008 this sequence version replaced NM_001081345.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGATGAGAA ATAAGGACAA AAGCCAAGAG GAGGACAGTT CGCTACACAG CAATGCATCG 
AGTCGTTCAG CTTCTGAAGA AGTGTCGGGG TCAGACTCAG GCAGTCAGTC GGAAAGTGAG 
CAGGGCAGCG AGCCCGGAAG TGGACATGGC AGCGAGTCCA ATAGCAGTTC GGAGTCTTCT 
GAGAGCCAAT CAGAGTCTGA GAGTGAATCG GCAGGTTCCA AATCCCAGCC AGTCCTTCCG 
GAAGCCAAAG AGAAGCCTGC CTCTAAGAAG GAACGGATAG CTGATGTGAA GAAGATGTGG 
GAAGAATATC CTGATGTTTA TGGGGTTAGG AGGTCAAATC GAAGCAGACA AGAACCATCA 
CGATTTAATG TTAAGGAAGA GGCAAGTAGC GGGTCTGAGA GTGGGAGTCC GAAAAGAAGA 
GGCCAGAGGC AGCTGAAAAA ACAAGAAAAA TGGAAACAGG ACCCCTCAGA AGACGAACAG 
GAACAGGGAA CCAGTGCAGA GAGTGAGGCA GAACAAAAGA AAGGAAAAGC TAGAAGACCG 
GTCCCTAGAA GAACAGTGCC CAAACCTCAA GTTAAAAAGC AACCTAAGAT TCAGCGTGGA 
AAGAGGAAAA AACAAGAGTC TTCAGATGAC GACGACGATG ATGATGAAGC TCCCAAAAGG 
CAGACTCGCC GAAGAGCTGC TAAAAACGTG AGTTACAAAG AAGATGATGA CTTTGAGACT 
GATTCAGATG ATCTCATTGA GATGACTGGA GAAGGAGGTG ATGAACAGCA AGATAATAGT 
GAAACTATTG AAAAGGTCTT AGATTCAAGA CTGGGAAAGA AAGGAGCCAC TGGAGCGTCT 
ACCACTGTGT ATGCTGTTGA AGCTAATGGA GATCCTAGCG ATGACTTTGA CACGGAGAGG 
GAGGAAGGTG AAGTCCAGTA CCTTATCAAG TGGAAGGGCT GGTCTTATAT TCACAGCACA 
TGGGAGAGTG AAGACTCCTT ACAGCAACAG AAAGTGAAGG GCCTAAAGAA ACTGGAGAAT 
TTCAAGAAGA AAGAGGATGA GGTCAAGCAA TGGCTAGGGA AAGTTTCTCC TGAAGATGTT 
GAATATTTCA GTTGCCAACA GGAGCTGGCA TCAGAGTTGA ATAAGCAGTA TCAGATAGTA 
GAGAGAGTAA TAGCTGTGAA GACAAGTAAA TCTACACTGG GCCAAACAGA TTTCCCAGCT 
CACAGTCGGA AGCCAGCACC TTCAAATGAG CCTGAGTATC TGTGCAAGTG GATGGGACTA 
CCCTACTCAG AGTGCAGCTG GGAAGATGAA GCCTTGATTG GAAAGAAATT CCAGAATTGT 
ATCGACAGCT TCCACAGTCG AAACAACTCC AAAACCATTC CCACAAGAGA ATGCAAGGCT 
CTAAAGCAAA GGCCGAGATT TGTGGCTTTG AAGAAGCAGC CAGCTTATTT AGGAGGGGAG 
AGTCTGGAGC TCCGAGACTA TCAGCTGGAG GGCCTCAACT GGCTGGCTCA CTCCTGGTGC 
AAAAGTAACA GTGTAATCCT TGCTGATGAA ATGGGCCTAG GAAAGACCAT CCAGACCATA 
TCGTTTCTCT CCTACCTGTT CCACCAGCAC CAGCTCTATG GGCCTTTTCT TATAGTTGTT 
CCCTTGTCCA CCCTTACCTC ATGGCAGAGA GAGTTTGAGA TCTGGGCACC AGAGATTAAT 
GTCGTGGTTT ACATAGGTGA CCTGATGAGC AGAAATACGA TAAGGGAATA TGAGTGGATT 
CATTCTCAAA CCAAAAGGTT GAAGTTCAAT GCACTTATAA CAACATATGA GATCCTCCTA 
AAAGATAAGA CCGTGCTGGG CAGTATTAAC TGGGCCTTCC TGGGAGTAGA TGAAGCCCAT 
CGGTTGAAGA ATGATGACTC TTTATTGTAT AAAACTCTGA TTGATTTCAA GTCCAACCAT 
AGGCTACTGA TCACGGGCAC CCCTCTTCAG AATTCCCTCA AGGAACTTTG GTCCTTGCTG 
CACTTTATTA TGCCGGAGAA GTTTGAGTTC TGGGAAGATT TTGAAGAAGA CCATGGAAAA 
GGGAGAGAGA ATGGCTACCA GAGTCTTCAT AAGGTACTAG AGCCTTTTCT TCTTCGAAGA 
GTCAAAAAAG ATGTGGAGAA ATCCCTTCCT GCCAAAGTGG AGCAGATTCT CAGGGTGGAG 
ATGTCAGCCC TTCAGAAACA GTATTATAAA TGGATCCTGA CCAGGAATTA CAAGGCTCTT 
GCCAAAGGGA CCAGAGGCAG CACATCTGGC TTCCTTAATA TCGTGATGGA GCTGAAAAAG 
TGCTGCAACC ACTGTTATCT GATCAAAGCT CCCGAGGACA GCGAGAGGGA GAGCGGCCAG 
GAGGTCCTTC AGTCTCTAAT TAGGAGCAGT GGGAAGCTGA TCCTGTTAGA CAAACTGTTG 
ACAAGACTCC GAGAAAGGGG AAACAGAGTC CTTATCTTCT CTCAGATGGT GAGGATGTTG 
GATATCCTGG CCGAGTACCT GACTATCAAA CACTATCCTT TCCAGCGCCT GGATGGTTCT 
ATCAAGGGGG AAATCCGAAA GCAGGCACTG GATCACTTCA ATGCAGATGG GTCTGAGGAC 
TTCTGTTTCC TGCTCTCCAC ACGGGCTGGC GGCCTGGGAA TCAATTTGGC TTCAGCGGAC 
ACAGTCGTCA TCTTTGACTC TGACTGGAAC CCGCAGAACG ACTTGCAGGC ACAAGCCCGA 
GCACATAGAA TCGGTCAGAA GAAGCAGGTG AATATTTACC GCCTGGTTAC AAAAGGGACT 
GTGGAAGAGG AGATCATAGA ACGGGCCAAA AAGAAGATGG TGTTGGACCA TCTGGTGATT 
CAGCGCATGG ATACCACTGG CAGGACGGTT CTTGAAAACA ACTCAGGAAG ATCCAACTCA 
AACCCTTTTA ATAAAGAAGA GCTGACAGCT ATTTTGAAAT TTGGAGCAGA GGATCTCTTC 
AAAGAAATTG AAGGAGAGGA GTCAGAGCCT CAGGAAATGG ATATAGATGA AATTTTGCGT 
TTAGCTGAAA CCAGAGAGAA TGAAGTGTCA ACTAGTGCGA CAGATGAGCT GCTCTCACAG 
TTTAAGGTTG CCAACTTTGC AACAATGGAA GATGAAGAGG AGCTAGAAGA GCGTCCTCAC 
AAAGACTGGG ATGAAATTAT TCCCGAAGAG CAAAGGAAAA AAGTGGAGGA GGAAGAACGG 
CAGAAGGAGC TGGAAGAAAT TTATATGCTG CCTCGGATTC GCAGTTCCAC TAAAAAGGCT 
CAGACAAACG ACAGTGATTC AGATACTGAG TCTAAGAGGC AGGCCCAGAG GTCTTCTGCT 
TCTGAGAGTG AGACGGACGA CTCTGATGAT GACAAGAAGC CGAAGCGAAG AGGGCGCCCC 
CGAAGTGTGC GCAAAGACCT TGTGGAGGGC TTCACGGATG CCGAGATCAG AAGGTTCATC 
AAGGCTTATA AGAAGTTTGG TCTCCCTCTT GAAAGGCTAG AGTGCATAGC ACGTGATGCT 
GAGCTAGTTG ACAAGTCTGT GGCAGACTTG AAACGCCTGG GGGAGCTGAT TCATAATAGC 
TGTGTGTCAG CAATGCAGGA ATATGAGGAG CAACTGAAAG AAAGCACCAG TGAAGGGAAA 
GGACCAGGCA AGAGAAGAGG CCCAACAATC AAGATCTCTG GGGTTCAGGT GAACGTGAAG 
TCCATTATCC AACACGAAGA AGAGTTTGAG ATGCTGCATA AATCTATCCC TGTGGACCCT 
GAGGAAAAAA AAAAGTACTG CCTAACCTGC CGAGTCAAAG CAGCACATTT TGACGTCGAG 
TGGGGCGTGG AGGACGATTC TCGTCTGTTG CTTGGAATCT ATGAACATGG CTATGGAAAT 
TGGGAGCTAA TTAAGACAGA CCCAGAGCTA AAGCTAACTG ATAAAATTCT ACCAGTGGAG 
ACGGATAAAA AGCCGCAGGG GAAGCAGCTG CAGACCCGAG TGGACTACTT GCTGAAGCTG 
CTCAGGAAGG GTCTGGAGAA GAAGGGCACC GTGGCCAGCG GAGAGGAGGC CAAACTAAAG 
AAGCGGAAGC CTCGAGTAAA GAAGGAGAAC AAAGCCCCCA GGCTGAAGGA CGAGCACGGG 
CTGGAGCCAG CGTCGCCTCG ACACTCCGAC AACCCATCTG AAGAGGGGGA AGTGAAGGAT 
GATGGTCTAG AAAAAAGTCC AACGAAAAAA AAGCAGAAGA AGAAAGAGAA CAAGGAGAAC 
AAAGAGAAAC CAGTGAGCTC TCGGAAGGAC AGGGAAGGAG ACAAAGAGAG GAAGAAGTCC 
AAAGACAAGA AAGAGAAGGT CAAAGGTGGT GATGGCAAGT CTTCAAGTAA ATCAAAGCGA 
TCTCAGGGTC CTGTGCACAT TACAGCAGGA AGTGAGCCTG TTCCCATCGG GGAGGATGAA 
GACGATGATC TGGACCAGGA AACCTTCAGC ATTTGTAAGG AGAGGATGAG GCCTGTGAAG 
AAGGCACTGA AACAGTTGGA CAAACCTGAC AAAGGGCTCA GTGTGCAAGA ACAGCTGGAA 
CACACCAGGA ACTGCCTGCT CAAAATTGGA GACCGCATAG CCGAGTGCCT TAAAGCTTAC 
TCAGACCAGG AGCACATCAA ATTGTGGAGG AGGAACCTAT GGATTTTTGT TTCCAAGTTT 
ACAGAATTTG ATGCACGAAA ACTACATAAG TTATATAAGA TGGCTCATAA GAAAAGATCT 
CAAGAAGAGG AGGAACAAAA GAAGAAAGAT GATTCGCTTG GTGGTAAGAA ACCATTTCGA 
CCAGAGGCCT CGGGCTCAAG CCGGGATTCT CTGATATCTC AGTCCCATAC CTCACACAAC 
CTTCATCCTC AGAAGCCTCA TTTGCCTGCC TCCCATGGCC CACAAATGCA TGGACATCCA 
AGAGATAACT ACAGTCACCC CAACAAGAGA CACTTTAGTA ACGCAGATCG AGGAGACTGG 
CAGAGGGAAA GAAAGTTCAA CTATGGTGGT GGTAACAGCG CCCCCTGGGG GGGCGACCGG 
CACCATCAGT ATGAGCAGCA TTGGTATAAG GACCACCACT ATGGTGACCG GAGGCATATG 
GATGCCCACC GTTCTGGGAG CTACCGCCCT AACAACATGT CCAGAAAGAG GCCGTATGAG 
CAGTACAACA GCGACCGAGA CCACCGGGGA CACAGAGACT ATTATGACAG GCACCATCAT 
GACTCCAAGC GGAGGAGGTC AGATGACTTC AGGCCCCAGA ATTATCACCA GCAGGATTTC 
CGACGAATGT CTGACCACAG ACCCACCATG GGTTACCACG GCCAGGGACC CTCAGACCAT 
TATCGCTCTT TCCACACAGA CAAGTTGGGG GAATATAAGC AGCCCATGCC TTCATTGCAC 
ACTGCGCTCT CAGACCCACG CTCACCCCCT TCCCAGAAAT CTCCCCATGA TTCCAAATCA 
CCCTTGGATC ATAGGTCTCC TTTGGAGAGA TCACTAGAAC AGAAAAACAA CCCAGATTAT 
AACTGGAATG TTCGGAAAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074814.2
CDS	573..6056
Translation	MMRNKDKSQEEDSSLHSNASSRSASEEVSGSDSGSQSESEQGSEPGSGHGSESNSSSESSESQSESESESAGSKSQPVLPEAKEKPASKKERIADVKKMWEEYPDVYGVRRSNRSRQEPSRFNVKEEASSGSESGSPKRRGQRQLKKQEKWKQDPSEDEQEQGTSAESEAEQKKGKARRPVPRRTVPKPQVKKQPKIQRGKRKKQESSDDDDDDDEAPKRQTRRRAAKNVSYKEDDDFETDSDDLIEMTGEGGDEQQDNSETIEKVLDSRLGKKGATGASTTVYAVEANGDPSDDFDTEREEGEVQYLIKWKGWSYIHSTWESEDSLQQQKVKGLKKLENFKKKEDEVKQWLGKVSPEDVEYFSCQQELASELNKQYQIVERVIAVKTSKSTLGQTDFPAHSRKPAPSNEPEYLCKWMGLPYSECSWEDEALIGKKFQNCIDSFHSRNNSKTIPTRECKALKQRPRFVALKKQPAYLGGESLELRDYQLEGLNWLAHSWCKSNSVILADEMGLGKTIQTISFLSYLFHQHQLYGPFLIVVPLSTLTSWQREFEIWAPEINVVVYIGDLMSRNTIREYEWIHSQTKRLKFNALITTYEILLKDKTVLGSINWAFLGVDEAHRLKNDDSLLYKTLIDFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFEFWEDFEEDHGKGRENGYQSLHKVLEPFLLRRVKKDVEKSLPAKVEQILRVEMSALQKQYYKWILTRNYKALAKGTRGSTSGFLNIVMELKKCCNHCYLIKAPEDSERESGQEVLQSLIRSSGKLILLDKLLTRLRERGNRVLIFSQMVRMLDILAEYLTIKHYPFQRLDGSIKGEIRKQALDHFNADGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGTVEEEIIERAKKKMVLDHLVIQRMDTTGRTVLENNSGRSNSNPFNKEELTAILKFGAEDLFKEIEGEESEPQEMDIDEILRLAETRENEVSTSATDELLSQFKVANFATMEDEEELEERPHKDWDEIIPEEQRKKVEEEERQKELEEIYMLPRIRSSTKKAQTNDSDSDTESKRQAQRSSASESETDDSDDDKKPKRRGRPRSVRKDLVEGFTDAEIRRFIKAYKKFGLPLERLECIARDAELVDKSVADLKRLGELIHNSCVSAMQEYEEQLKESTSEGKGPGKRRGPTIKISGVQVNVKSIIQHEEEFEMLHKSIPVDPEEKKKYCLTCRVKAAHFDVEWGVEDDSRLLLGIYEHGYGNWELIKTDPELKLTDKILPVETDKKPQGKQLQTRVDYLLKLLRKGLEKKGTVASGEEAKLKKRKPRVKKENKAPRLKDEHGLEPASPRHSDNPSEEGEVKDDGLEKSPTKKKQKKKENKENKEKPVSSRKDREGDKERKKSKDKKEKVKGGDGKSSSKSKRSQGPVHITAGSEPVPIGEDEDDDLDQETFSICKERMRPVKKALKQLDKPDKGLSVQEQLEHTRNCLLKIGDRIAECLKAYSDQEHIKLWRRNLWIFVSKFTEFDARKLHKLYKMAHKKRSQEEEEQKKKDDSLGGKKPFRPEASGSSRDSLISQSHTSHNLHPQKPHLPASHGPQMHGHPRDNYSHPNKRHFSNADRGDWQRERKFNYGGGNSAPWGGDRHHQYEQHWYKDHHYGDRRHMDAHRSGSYRPNNMSRKRPYEQYNSDRDHRGHRDYYDRHHHDSKRRRSDDFRPQNYHQQDFRRMSDHRPTMGYHGQGPSDHYRSFHTDKLGEYKQPMPSLHTALSDPRSPPSQKSPHDSKSPLDHRSPLERSLEQKNNPDYNWNVRKT

Target ORF information:

RefSeq Version	NM_001081345.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 2 (Chd2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081345.2

ORF Insert Sequence:

ATGATGAGAA ATAAGGACAA AAGCCAAGAG GAGGACAGTT CGCTACACAG CAATGCATCG 
AGTCGTTCAG CTTCTGAAGA AGTGTCGGGG TCAGACTCAG GCAGTCAGTC GGAAAGTGAG 
CAGGGCAGCG AGCCCGGAAG TGGACATGGC AGCGAGTCCA ATAGCAGTTC GGAGTCTTCT 
GAGAGCCAAT CAGAGTCTGA GAGTGAATCG GCAGGTTCCA AATCCCAGCC AGTCCTTCCG 
GAAGCCAAAG AGAAGCCTGC CTCTAAGAAG GAACGGATAG CTGATGTGAA GAAGATGTGG 
GAAGAATATC CTGATGTTTA TGGGGTTAGG AGGTCAAATC GAAGCAGACA AGAACCATCA 
CGATTTAATG TTAAGGAAGA GGCAAGTAGC GGGTCTGAGA GTGGGAGTCC GAAAAGAAGA 
GGCCAGAGGC AGCTGAAAAA ACAAGAAAAA TGGAAACAGG ACCCCTCAGA AGACGAACAG 
GAACAGGGAA CCAGTGCAGA GAGTGAGGCA GAACAAAAGA AAGGAAAAGC TAGAAGACCG 
GTCCCTAGAA GAACAGTGCC CAAACCTCAA GTTAAAAAGC AACCTAAGAT TCAGCGTGGA 
AAGAGGAAAA AACAAGAGTC TTCAGATGAC GACGACGATG ATGATGAAGC TCCCAAAAGG 
CAGACTCGCC GAAGAGCTGC TAAAAACGTG AGTTACAAAG AAGATGATGA CTTTGAGACT 
GATTCAGATG ATCTCATTGA GATGACTGGA GAAGGAGGTG ATGAACAGCA AGATAATAGT 
GAAACTATTG AAAAGGTCTT AGATTCAAGA CTGGGAAAGA AAGGAGCCAC TGGAGCGTCT 
ACCACTGTGT ATGCTGTTGA AGCTAATGGA GATCCTAGCG ATGACTTTGA CACGGAGAGG 
GAGGAAGGTG AAGTCCAGTA CCTTATCAAG TGGAAGGGCT GGTCTTATAT TCACAGCACA 
TGGGAGAGTG AAGACTCCTT ACAGCAACAG AAAGTGAAGG GCCTAAAGAA ACTGGAGAAT 
TTCAAGAAGA AAGAGGATGA GGTCAAGCAA TGGCTAGGGA AAGTTTCTCC TGAAGATGTT 
GAATATTTCA GTTGCCAACA GGAGCTGGCA TCAGAGTTGA ATAAGCAGTA TCAGATAGTA 
GAGAGAGTAA TAGCTGTGAA GACAAGTAAA TCTACACTGG GCCAAACAGA TTTCCCAGCT 
CACAGTCGGA AGCCAGCACC TTCAAATGAG CCTGAGTATC TGTGCAAGTG GATGGGACTA 
CCCTACTCAG AGTGCAGCTG GGAAGATGAA GCCTTGATTG GAAAGAAATT CCAGAATTGT 
ATCGACAGCT TCCACAGTCG AAACAACTCC AAAACCATTC CCACAAGAGA ATGCAAGGCT 
CTAAAGCAAA GGCCGAGATT TGTGGCTTTG AAGAAGCAGC CAGCTTATTT AGGAGGGGAG 
AGTCTGGAGC TCCGAGACTA TCAGCTGGAG GGCCTCAACT GGCTGGCTCA CTCCTGGTGC 
AAAAGTAACA GTGTAATCCT TGCTGATGAA ATGGGCCTAG GAAAGACCAT CCAGACCATA 
TCGTTTCTCT CCTACCTGTT CCACCAGCAC CAGCTCTATG GGCCTTTTCT TATAGTTGTT 
CCCTTGTCCA CCCTTACCTC ATGGCAGAGA GAGTTTGAGA TCTGGGCACC AGAGATTAAT 
GTCGTGGTTT ACATAGGTGA CCTGATGAGC AGAAATACGA TAAGGGAATA TGAGTGGATT 
CATTCTCAAA CCAAAAGGTT GAAGTTCAAT GCACTTATAA CAACATATGA GATCCTCCTA 
AAAGATAAGA CCGTGCTGGG CAGTATTAAC TGGGCCTTCC TGGGAGTAGA TGAAGCCCAT 
CGGTTGAAGA ATGATGACTC TTTATTGTAT AAAACTCTGA TTGATTTCAA GTCCAACCAT 
AGGCTACTGA TCACGGGCAC CCCTCTTCAG AATTCCCTCA AGGAACTTTG GTCCTTGCTG 
CACTTTATTA TGCCGGAGAA GTTTGAGTTC TGGGAAGATT TTGAAGAAGA CCATGGAAAA 
GGGAGAGAGA ATGGCTACCA GAGTCTTCAT AAGGTACTAG AGCCTTTTCT TCTTCGAAGA 
GTCAAAAAAG ATGTGGAGAA ATCCCTTCCT GCCAAAGTGG AGCAGATTCT CAGGGTGGAG 
ATGTCAGCCC TTCAGAAACA GTATTATAAA TGGATCCTGA CCAGGAATTA CAAGGCTCTT 
GCCAAAGGGA CCAGAGGCAG CACATCTGGC TTCCTTAATA TCGTGATGGA GCTGAAAAAG 
TGCTGCAACC ACTGTTATCT GATCAAAGCT CCCGAGGACA GCGAGAGGGA GAGCGGCCAG 
GAGGTCCTTC AGTCTCTAAT TAGGAGCAGT GGGAAGCTGA TCCTGTTAGA CAAACTGTTG 
ACAAGACTCC GAGAAAGGGG AAACAGAGTC CTTATCTTCT CTCAGATGGT GAGGATGTTG 
GATATCCTGG CCGAGTACCT GACTATCAAA CACTATCCTT TCCAGCGCCT GGATGGTTCT 
ATCAAGGGGG AAATCCGAAA GCAGGCACTG GATCACTTCA ATGCAGATGG GTCTGAGGAC 
TTCTGTTTCC TGCTCTCCAC ACGGGCTGGC GGCCTGGGAA TCAATTTGGC TTCAGCGGAC 
ACAGTCGTCA TCTTTGACTC TGACTGGAAC CCGCAGAACG ACTTGCAGGC ACAAGCCCGA 
GCACATAGAA TCGGTCAGAA GAAGCAGGTG AATATTTACC GCCTGGTTAC AAAAGGGACT 
GTGGAAGAGG AGATCATAGA ACGGGCCAAA AAGAAGATGG TGTTGGACCA TCTGGTGATT 
CAGCGCATGG ATACCACTGG CAGGACGGTT CTTGAAAACA ACTCAGGAAG ATCCAACTCA 
AACCCTTTTA ATAAAGAAGA GCTGACAGCT ATTTTGAAAT TTGGAGCAGA GGATCTCTTC 
AAAGAAATTG AAGGAGAGGA GTCAGAGCCT CAGGAAATGG ATATAGATGA AATTTTGCGT 
TTAGCTGAAA CCAGAGAGAA TGAAGTGTCA ACTAGTGCGA CAGATGAGCT GCTCTCACAG 
TTTAAGGTTG CCAACTTTGC AACAATGGAA GATGAAGAGG AGCTAGAAGA GCGTCCTCAC 
AAAGACTGGG ATGAAATTAT TCCCGAAGAG CAAAGGAAAA AAGTGGAGGA GGAAGAACGG 
CAGAAGGAGC TGGAAGAAAT TTATATGCTG CCTCGGATTC GCAGTTCCAC TAAAAAGGCT 
CAGACAAACG ACAGTGATTC AGATACTGAG TCTAAGAGGC AGGCCCAGAG GTCTTCTGCT 
TCTGAGAGTG AGACGGACGA CTCTGATGAT GACAAGAAGC CGAAGCGAAG AGGGCGCCCC 
CGAAGTGTGC GCAAAGACCT TGTGGAGGGC TTCACGGATG CCGAGATCAG AAGGTTCATC 
AAGGCTTATA AGAAGTTTGG TCTCCCTCTT GAAAGGCTAG AGTGCATAGC ACGTGATGCT 
GAGCTAGTTG ACAAGTCTGT GGCAGACTTG AAACGCCTGG GGGAGCTGAT TCATAATAGC 
TGTGTGTCAG CAATGCAGGA ATATGAGGAG CAACTGAAAG AAAGCACCAG TGAAGGGAAA 
GGACCAGGCA AGAGAAGAGG CCCAACAATC AAGATCTCTG GGGTTCAGGT GAACGTGAAG 
TCCATTATCC AACACGAAGA AGAGTTTGAG ATGCTGCATA AATCTATCCC TGTGGACCCT 
GAGGAAAAAA AAAAGTACTG CCTAACCTGC CGAGTCAAAG CAGCACATTT TGACGTCGAG 
TGGGGCGTGG AGGACGATTC TCGTCTGTTG CTTGGAATCT ATGAACATGG CTATGGAAAT 
TGGGAGCTAA TTAAGACAGA CCCAGAGCTA AAGCTAACTG ATAAAATTCT ACCAGTGGAG 
ACGGATAAAA AGCCGCAGGG GAAGCAGCTG CAGACCCGAG TGGACTACTT GCTGAAGCTG 
CTCAGGAAGG GTCTGGAGAA GAAGGGCACC GTGGCCAGCG GAGAGGAGGC CAAACTAAAG 
AAGCGGAAGC CTCGAGTAAA GAAGGAGAAC AAAGCCCCCA GGCTGAAGGA CGAGCACGGG 
CTGGAGCCAG CGTCGCCTCG ACACTCCGAC AACCCATCTG AAGAGGGGGA AGTGAAGGAT 
GATGGTCTAG AAAAAAGTCC AACGAAAAAA AAGCAGAAGA AGAAAGAGAA CAAGGAGAAC 
AAAGAGAAAC CAGTGAGCTC TCGGAAGGAC AGGGAAGGAG ACAAAGAGAG GAAGAAGTCC 
AAAGACAAGA AAGAGAAGGT CAAAGGTGGT GATGGCAAGT CTTCAAGTAA ATCAAAGCGA 
TCTCAGGGTC CTGTGCACAT TACAGCAGGA AGTGAGCCTG TTCCCATCGG GGAGGATGAA 
GACGATGATC TGGACCAGGA AACCTTCAGC ATTTGTAAGG AGAGGATGAG GCCTGTGAAG 
AAGGCACTGA AACAGTTGGA CAAACCTGAC AAAGGGCTCA GTGTGCAAGA ACAGCTGGAA 
CACACCAGGA ACTGCCTGCT CAAAATTGGA GACCGCATAG CCGAGTGCCT TAAAGCTTAC 
TCAGACCAGG AGCACATCAA ATTGTGGAGG AGGAACCTAT GGATTTTTGT TTCCAAGTTT 
ACAGAATTTG ATGCACGAAA ACTACATAAG TTATATAAGA TGGCTCATAA GAAAAGATCT 
CAAGAAGAGG AGGAACAAAA GAAGAAAGAT GATTCGCTTG GTGGTAAGAA ACCATTTCGA 
CCAGAGGCCT CGGGCTCAAG CCGGGATTCT CTGATATCTC AGTCCCATAC CTCACACAAC 
CTTCATCCTC AGAAGCCTCA TTTGCCTGCC TCCCATGGCC CACAAATGCA TGGACATCCA 
AGAGATAACT ACAGTCACCC CAACAAGAGA CACTTTAGTA ACGCAGATCG AGGAGACTGG 
CAGAGGGAAA GAAAGTTCAA CTATGGTGGT GGTAACAGCG CCCCCTGGGG GGGCGACCGG 
CACCATCAGT ATGAGCAGCA TTGGTATAAG GACCACCACT ATGGTGACCG GAGGCATATG 
GATGCCCACC GTTCTGGGAG CTACCGCCCT AACAACATGT CCAGAAAGAG GCCGTATGAG 
CAGTACAACA GCGACCGAGA CCACCGGGGA CACAGAGACT ATTATGACAG GCACCATCAT 
GACTCCAAGC GGAGGAGGTC AGATGACTTC AGGCCCCAGA ATTATCACCA GCAGGATTTC 
CGACGAATGT CTGACCACAG ACCCACCATG GGTTACCACG GCCAGGGACC CTCAGACCAT 
TATCGCTCTT TCCACACAGA CAAGTTGGGG GAATATAAGC AGCCCATGCC TTCATTGCAC 
ACTGCGCTCT CAGACCCACG CTCACCCCCT TCCCAGAAAT CTCCCCATGA TTCCAAATCA 
CCCTTGGATC ATAGGTCTCC TTTGGAGAGA TCACTAGAAC AGAAAAACAA CCCAGATTAT 
AACTGGAATG TTCGGAAAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chd2 regulates chromatin for proper gene expression toward differentiation in mouse embryonic stem cells.
Nucleic acids research45(15)8758-8772(2017 Sep)
Semba Y,Harada A,Maehara K,Oki S,Meno C,Ueda J,Yamagata K,Suzuki A,Onimaru M,Nogami J,Okada S,Akashi K,Ohkawa Y

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

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Chd2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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