chd2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chd2
Entrez Gene ID	569795
Full Name	chromodomain helicase DNA binding protein 2
Synonyms	si:ch211-196l7.5
General protein information	Preferred Names chromodomain helicase DNA binding protein 2 Names chromodomain-helicase-DNA-binding protein 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021467690.1	XP_021323365.1	chromodomain-helicase-DNA-binding protein 2 isoform X1
XM_021467692.1	XP_021323367.1	chromodomain-helicase-DNA-binding protein 2 isoform X3
XM_009293623.2	XP_009291898.1	chromodomain-helicase-DNA-binding protein 2 isoform X1
NM_001281462.1	NP_001268391.1	chromodomain-helicase-DNA-binding protein 2
XM_009293623.3	XP_009291898.1	chromodomain-helicase-DNA-binding protein 2 isoform X2
XM_021467691.1	XP_021323366.1	chromodomain-helicase-DNA-binding protein 2 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CHD2	XP_413879.3
Danio rerio (zebrafish)	chd2	XP_005159227.1
Oryza sativa (rice)	Os07g0660200	NP_001060530.1
Canis lupus familiaris (dog)	CHD2	XP_005618381.1
Mus musculus (house mouse)	Chd2	NP_001074814.2
Caenorhabditis elegans (roundworm)	chd-1	NP_491994.2
	CHD2	XP_002808499.1
Rattus norvegicus (Norway rat)	Chd2	NP_001100993.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008698	XP_314800.4
Homo sapiens (human)	CHD2	NP_001262.3
Drosophila melanogaster (fruit fly)	Chd1	NP_477197.1
Xenopus tropicalis (tropical clawed frog)	chd2	XP_002933353.1
Pan troglodytes (chimpanzee)	CHD2	XP_001170676.2
Arabidopsis thaliana (thale cress)	CHR5	NP_178970.3

Gene Ontology
Bibliography

Related articles in PubMed

Rescue of neural crest-derived phenotypes in a zebrafish CHARGE model by Sox10 downregulation.
Asad Z, Pandey A, Babu A, Sun Y, Shevade K, Kapoor S, Ullah I, Ranjan S, Scaria V, Bajpai R, Sachidanandan C
Human molecular genetics25(16)3539-3554(2016 08)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

De novo loss-of-function mutations in CHD2 cause a fever-sensitive myoclonic epileptic encephalopathy sharing features with Dravet syndrome.
Suls A, Jaehn JA, Kecsk?s A, Weber Y, Weckhuysen S, Craiu DC, Siekierska A, Dj?mi? T, Afrikanova T, Gormley P, von Spiczak S, Kluger G, Iliescu CM, Talvik T, Talvik I, Meral C, Caglayan HS, Giraldez BG, Serratosa J, Lemke JR, Hoffman-Zacharska D, Szczepanik E, Barisic N, Komarek V, Hjalgrim H, M?ller RS, Linnankivi T, Dimova P, Striano P, Zara F, Marini C, Guerrini R, Depienne C, Baulac S, Kuhlenb?umer G, Crawford AD, Lehesjoki AE, de Witte PA, Palotie A, Lerche H, Esguerra CV, De Jonghe P, Helbig I,
American journal of human genetics93(5)967-75(2013 Nov)

Consequences of lineage-specific gene loss on functional evolution of surviving paralogs: ALDH1A and retinoic acid signaling in vertebrate genomes.
Ca?estro C, Catchen JM, Rodr?guez-Mar? A, Yokoi H, Postlethwait JH
PLoS genetics5(5)e1000496(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

chd2-knockdown larvae exhibited altered locomotor activity
Title: De novo loss-of-function mutations in CHD2 cause a fever-sensitive myoclonic epileptic encephalopathy sharing features with Dravet syndrome.

The following chd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36139	NM_001281462.1 Latest version!	Danio rerio chromodomain helicase DNA binding protein 2 (chd2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
ODa45852	XM_021467691.1 Latest version!	Danio rerio chromodomain helicase DNA binding protein 2 (chd2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa45852	XM_021467690.1 Latest version!	Danio rerio chromodomain helicase DNA binding protein 2 (chd2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa45854	XM_021467692.1 Latest version!	Danio rerio chromodomain helicase DNA binding protein 2 (chd2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa45853	XM_009293623.3 Latest version!	Danio rerio chromodomain helicase DNA binding protein 2 (chd2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa45853	XM_009293623.2	Danio rerio chromodomain helicase DNA binding protein 2 (chd2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chd2 ( NM_001281462.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001268391.1 Danio rerio chromodomain helicase DNA binding protein 2 (chd2), mRNA.

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CloneID	ODa36139
Clone ID Related Accession (Same CDS sequence)	NM_001281462.1
Accession Version	NM_001281462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	chromodomain-helicase-DNA-binding protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX649578.14. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGATGAAGA ATAAGAGTAA AAAGCAAGAA GACGAAGGCT CGACTCAAAG CAATGCTTCC 
AGCAACTCAG CATCCGAGGA ATCCAACCGC TCTGGGTCGG AGTCTGGAAG TCAGTCGGAG 
AGCGAGCAGG GCAGTGAAAA AGCACAATCC CGGCATTCTG AATCCAACAG CACGTCTGAT 
TCTGAGAGCC ACTCTGAGTC AGGCAGCGAA TCCAATGGGT CCAAATCTCA TCAGAGCTCA 
GCGCAGGTCA AAGACAAACC TGTCAGGAAG AAAGACAACC TGGCGGATGT GAAGAAGATG 
TGGGAAGAAC ATCCTGATGT TTATGGTGTG AGAAGATCCA GTCGCAGTAG ACAAGAGCCT 
GCACGCCTTA ATATTGGAGC TGAGGGCAGC AGTGATTCAG AGGGAGAAAG TTCCAAACGA 
AAAAGCTCTC GGCAGAAAAA GAAAGAAAAT ACCTGGAAAG ATGATGACTC AAATGACGAA 
GAGGATGTTG AGGAAGAGAC CAGCAGTTCA GAGAGTGAGC AGGAAGAGAA AAAAGTTAGA 
TCCAGACGAC TTCCTGCTAG AAGACCACAG ACCAAATCAT CTGCTAGTAA ACAGTCACAA 
AAGGGACGAA AACAGAGAAA GCAGGAGTCC TCAGATGAAG ATGATGACGA TGATGATGAT 
GACGAGGAGG ACACTCCAAA AAGACAAACA CGGCGGCGGG CTGCTACTAA AGTCAGTTAC 
AAAGAGGACC AGAATGATTT TGAGACGGAC TCTGATGACC TTATTGAAAT GGCAGAAGGG 
GCAGAGGAGC AGCAAGATGA TGACAGTGAG ACCATTGAGA AGGTTGTTGA CACTAGGACT 
GGCAAGAAAG GAGCTACTGG AGCTTCCACA ACTCTCTATG CCATTGAGGA AAACGGGGAC 
CCTGGAGCAG ACTTTGACCC GGAGAAAGAG GAAGGAGAAA CTCAGTTCCT CATCAAGTGG 
AAAGGCTGGT CCTACATCCA CAACACCTGG GAGAGTGTGG ATTCCCTCAC TCAGCAGAAA 
GTCAAAGGCA TGAAGAAGCT GGAAAACTTC AAGAAGAAAA ATGAAGAGCT TAATGCCTGG 
TTGAAAAAAG CATCCCCTGA GGATTTAGAA TATTACAACT GTCAGCAAGA GCTCACAAAC 
GACCTGAGCA AGCAGTTCCA GATAATAGAG AGGGTGATTG CTCATAAAAG CAGCTCTGGT 
GAGCCCGAGT ACCTGTGTAA ATGGATGGGT CTGCCGTACG CTGAGTGCAC ATGGGAGGAC 
AGCGCTTTAA TCAAAAAGAA ATTTCAGGCC TGCATTGACA GTTTCCACAA CAGAAACAGC 
TCCAAGACTG TCCCTTCCAA AGACTGTAAG GTGTTGAAGC AGAGGCCAAG ATTTGTGCCC 
TTAAAGAAGC AGCCATCGTA CATTGGAGAT GAGAATCTTG AACTCAGAGA TTACCAGCTA 
GATGGAGTGA ACTGGCTGGC ACACTCCTGG TGCAGGTGTA ACAGTGTTAT CTTGGCTGAT 
GAAATGGGTT TAGGAAAGAC CATTCAGACG ATCTCCTTCC TCTCCTACCT TTTTCATCAG 
CACCAACTTT ACGGCCCCTT CATCGTGGTG GTGCCCCTGT CGACCCTCAC GTCCTGGCAG 
AGGGAGTTTG ACACTTGGGC CCCGGACATG AATGTGGTGG TGTATCTGGG AGATGTCACA 
AGTAGAAAGA CAATCCGAGA CTATGAATGG ATCAATCCAC AGACAAAAAG GATCAAGTTT 
AATGCACTTT TGACCACATA TGAAATTCTA CTGAAGGACA AGGGAGTGCT GGGGAATATA 
AACTGGGCTT TCCTTGGAGT AGATGAAGCT CACCGGCTTA AGAATGACGA CTCGCTGCTG 
TATAAAACTC TGATCGACTT TCGATCCAAT CACAGGCTGC TTATTACCGG GACTCCTCTG 
CAAAACTCCC TCAAAGAGCT GTGGTCCCTT CTGCACTTTC TGATGTCTGA CAAGTTTGAG 
TCCTGGGAGG ATTTTGAGGA TGAGCATGGT AAAGGCAGGG ATAATGGTTA TCAGAGTTTG 
CACAAAGTTC TAGAGCCTTT CCTGCTACGT CGTGTCAAGA AAGATGTGGA GAAATCCCTG 
CCGGCTAAAG TGGAGCAAAT CCTCAGAGTC GACATGTCAG CCCAGCAAAA ACAGTTCTAC 
AAATGGATCC TAACAAGAAA CTTCAAGGCC TTGTCTAAAG GCACTAGAGG CAGCTCTTCT 
GGCTTTCTGA ACATTGTCAT GGAGCTGAAG AAGTGTTGTA ACCACGCTTT CCTCATCAAA 
CAACCAGAAG ATGGAGAAAA TGATGCTCCA CAAGAGCATC TACAGAGTTT GGTGCGTGGT 
GGAGGGAAAC TGGTTCTCCT AGACAAGCTT CTCACACGAC TGAAGGACAG AGGCAACCGT 
GTGCTTATCT TCTCTCAGAT GGTCCGCATG TTGGACATTC TTGCTGATTA CTTGTCTATG 
AAACGATATC AATTCCAGCG TTTGGACGGA TCCATCAAGG GTGAGCTCCG AAAACAAGCA 
TTGGACCACT TTAATGCAGA AGGCTCTGAG GACTTCTGTT TCCTGCTGTC CACCCGAGCC 
GGAGGGCTTG GGATTAACTT GGCCTCTGCT GACACAGTGG TGATCTTTGA CTCTGACTGG 
AACCCACAGA ATGATCTTCA AGCTCAGGCC AGAGCCCACA GGATTGGCCA GAAAAAGCAA 
GTGAATATCT ATCGTCTGGT CACAAAAGGG ACTGTGGAGG AGGACATTAT TGAAAGAGCT 
AAAAAGAAGA TGGTTCTAGA CCATCTTGTC ATTCAGAGAA TGGACACCAC TGGTCGAACC 
GTACTGGATA ACAGTTCAGC AAATTCAAAT TCAAATCCCT TCAATAAGGA AGAACTGTCA 
GCGATCTTGA AGTTTGGAGC AGAGGATCTG TTTAAGGAAG CTGAGGGGGA GGAATCAGAA 
CCGCAGGAAA TGGACATCGA TGAGATATTG CGGCTTGCTG AAACCAGAGA GAGCGATCAG 
GGATCCAGTG CTACCGATGA GCTTCTGTCT CAGTTTAAGG TGGCTAACTT CACCATGGAT 
GAAAGCACAC CAGATCTTGA TGAAAAACCT GGCCGGGACT GGGATGAAAT AATTCCAGAA 
GAGCAGCGGC GGAAGGTGGA GGAAGAGCAG AAGCAGAAGG AGATGGAGGA TATCTACATG 
CTGCCAAGAA GCAGAAGCTC CAACAAGAAG GCACAAGCCA ATGACAGCGA CAGTGATGTG 
GGATCCAAAC TAAAGCACAG ATCCTCAGGT TCAGACAGTG AAACCGATGA CAGCGAGGAT 
GACAAGCGGC CTAAGAGGCG AGGAAGGCCA AGAGCCCGCA AAAACAACGT GGAGGGATTT 
ACTGATGCTG AGATCCGCAG GTTTATAAAA GCATACAAGA AATTTGGAGC TCCACTTGAG 
AGATTGGAGG CGATTGCCCG TGACTCTGAG CTGGTGGAGA AGTCCATTGC AGACCTGAAG 
CGACTAGGGG AGCTGGTGCA CAACAGCTGC GTGACTGCAG TACAGGAGCA CGAAGAGCAC 
TTGAAGGAAA ACCCTGTGGA AGCTAAAGGC CCAGGGAAAA GACGAGGCAT TAACATAAAA 
ATCTCTGGAG TTCAGGTCAA TGCTAAATCA ATTATCCAGC ATGAAGAGGA GTTTGAGCCC 
CTGCATAAAG CTGTGCCTTC AAATCCTGCT GAAAGGAACA AATTTCAGTT GACCTGCCGG 
GTGAAAACTC CTCACTTTGA TGTGGACTGG GATATTCACG ATGACACACA GTTGTTATTG 
GGAATATATG AGCACGGCTA CGGCAACTGG GACTTGATCA AGACTGATCC TGACCTCAAA 
CTTTCAGAGA AGATTCTTCT TGATGACCCT GAAAAGAAAC CTCAAGGAAA GCAGTTACAG 
ATGCGAACAG ACTACTTGCT GAAGATGCTG AAGAAAGACC TGGAAAGCCA AGATGCCATT 
AAAGCAGGAG ATGAGCCCAA AATTAAAAAG AGAAAACCTC GGATGAAGAA GAAGGCCCCT 
AGCAATGAAC TGGTCAATGA AATCGCTTCT CCTCGTCTAT CCGACAACCC ATCAGAAGAG 
GGAGAGGTAA AGGATGATGG AGCTGAAAAA ACACCCACAA AGAAGAAACA GAAAAAGGAC 
AACAAAGAGA ACAAAGAAAA AACAGGGACT CCTAAAAAAG AGAAGGAGGG GGACAAAGAC 
AAAAAGCGTT CCAAGCCCAA AAAAGAAAAG GCCAAACCAG CAGGAAAAGG GAAGAAAGCC 
CAGGGCCCTG TGCACATCAC TCAAGGAAGT GAACCTATAC CAATTGGAGA GGAAGATGAT 
GAGCTGGACC AGGAAACATT TAGCATTTGT AAGGAGCGGA TGCGGCCAGT GAAGAAAGCT 
CTTAAGCAGT TGGATAAGCC AGACGAGGGG CTGTCGGTGC AGGACCAGCT GCAGCACACA 
CGTACCTGTC TGCTGAAGAT CGGAGACCGC ATTACAGAGT GCCTTAAGTC ATATAGTGAC 
CCCGAGCACA TCAAGATCTG GCGCAGAAAC CTCTGGATAT TTGTGTCCAA ATTTACTGAG 
TTTGGTGCGC AGAAGCTGCA TAGGCTATAT AAGAAGGCTC AGAAGAAGCG CTCCCAGGAG 
GGAGAGAAAG AGCATAAGAA AAAAGACGAT CCTATTAAGA AAAAGCCCTT TAGGCCTGAA 
CCGTCTGGAT CTAGCAGGGA TTCAACGGGT GCCCAGCCTA CAACCAAACC CCATCCCCCT 
GTCAGCCATC AATCTGGACC TCCTCATCCT CCACACCATA GAGAACAGTA CAACCAGCAT 
AATAAGAGGC ATTTTCCTAA TGATGACAGG GGAGATTGGC AAAGGGACCG CAAATATGGC 
TACCCAGGTA ACAGCAATCA GCCCTGGCAA GGAGACCGTC ATCACCCTTA TGAGCCTCAC 
AGGTACAAGG ATCATCACTA CAGTGACCGT CGTCCTCATG GTGACTACCG CAGCTCTGGA 
GGATACCGTA ACAGTGGCTC CCCTCGCAAA CGGCCCTATG ACCAGTATGG CAACGATAGA 
GATCATCGGG GTCATCGAGA CTATTATGAC AGACATCCGG ACCCAAAGAG AAGGCGCTCG 
GATGAATATC GCTCTCCAAA CTACCACCAA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA 
GGAGGAGGGC ATCCGCAGGA CTTTAGAAGA ATGCCTGACC ACAGGGGACC TCCTGGAAAC 
CATGGCCCCA TGGGGCCGGA TCACTACCGG CCCTTTCACC CAGACAAACC CCCGCCTCTG 
ATTGACCCAC GTTCTCCGCA GTCTCAGAAA TCGCCTCTTG AGCCGATCTC ACCTGGTATG 
GACAGAAGTG CAGAGCAGAA ATCTGCGACA GACTTTAACT GGAACAACAG GAAGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268391.1
CDS	609..6008
Translation	MMKNKSKKQEDEGSTQSNASSNSASEESNRSGSESGSQSESEQGSEKAQSRHSESNSTSDSESHSESGSESNGSKSHQSSAQVKDKPVRKKDNLADVKKMWEEHPDVYGVRRSSRSRQEPARLNIGAEGSSDSEGESSKRKSSRQKKKENTWKDDDSNDEEDVEEETSSSESEQEEKKVRSRRLPARRPQTKSSASKQSQKGRKQRKQESSDEDDDDDDDDEEDTPKRQTRRRAATKVSYKEDQNDFETDSDDLIEMAEGAEEQQDDDSETIEKVVDTRTGKKGATGASTTLYAIEENGDPGADFDPEKEEGETQFLIKWKGWSYIHNTWESVDSLTQQKVKGMKKLENFKKKNEELNAWLKKASPEDLEYYNCQQELTNDLSKQFQIIERVIAHKSSSGEPEYLCKWMGLPYAECTWEDSALIKKKFQACIDSFHNRNSSKTVPSKDCKVLKQRPRFVPLKKQPSYIGDENLELRDYQLDGVNWLAHSWCRCNSVILADEMGLGKTIQTISFLSYLFHQHQLYGPFIVVVPLSTLTSWQREFDTWAPDMNVVVYLGDVTSRKTIRDYEWINPQTKRIKFNALLTTYEILLKDKGVLGNINWAFLGVDEAHRLKNDDSLLYKTLIDFRSNHRLLITGTPLQNSLKELWSLLHFLMSDKFESWEDFEDEHGKGRDNGYQSLHKVLEPFLLRRVKKDVEKSLPAKVEQILRVDMSAQQKQFYKWILTRNFKALSKGTRGSSSGFLNIVMELKKCCNHAFLIKQPEDGENDAPQEHLQSLVRGGGKLVLLDKLLTRLKDRGNRVLIFSQMVRMLDILADYLSMKRYQFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGTVEEDIIERAKKKMVLDHLVIQRMDTTGRTVLDNSSANSNSNPFNKEELSAILKFGAEDLFKEAEGEESEPQEMDIDEILRLAETRESDQGSSATDELLSQFKVANFTMDESTPDLDEKPGRDWDEIIPEEQRRKVEEEQKQKEMEDIYMLPRSRSSNKKAQANDSDSDVGSKLKHRSSGSDSETDDSEDDKRPKRRGRPRARKNNVEGFTDAEIRRFIKAYKKFGAPLERLEAIARDSELVEKSIADLKRLGELVHNSCVTAVQEHEEHLKENPVEAKGPGKRRGINIKISGVQVNAKSIIQHEEEFEPLHKAVPSNPAERNKFQLTCRVKTPHFDVDWDIHDDTQLLLGIYEHGYGNWDLIKTDPDLKLSEKILLDDPEKKPQGKQLQMRTDYLLKMLKKDLESQDAIKAGDEPKIKKRKPRMKKKAPSNELVNEIASPRLSDNPSEEGEVKDDGAEKTPTKKKQKKDNKENKEKTGTPKKEKEGDKDKKRSKPKKEKAKPAGKGKKAQGPVHITQGSEPIPIGEEDDELDQETFSICKERMRPVKKALKQLDKPDEGLSVQDQLQHTRTCLLKIGDRITECLKSYSDPEHIKIWRRNLWIFVSKFTEFGAQKLHRLYKKAQKKRSQEGEKEHKKKDDPIKKKPFRPEPSGSSRDSTGAQPTTKPHPPVSHQSGPPHPPHHREQYNQHNKRHFPNDDRGDWQRDRKYGYPGNSNQPWQGDRHHPYEPHRYKDHHYSDRRPHGDYRSSGGYRNSGSPRKRPYDQYGNDRDHRGHRDYYDRHPDPKRRRSDEYRSPNYHQGGGGGGGGGGGGGHPQDFRRMPDHRGPPGNHGPMGPDHYRPFHPDKPPPLIDPRSPQSQKSPLEPISPGMDRSAEQKSATDFNWNNRKT

Target ORF information:

RefSeq Version	NM_001281462.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 2 (chd2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281462.1

ORF Insert Sequence:

ATGATGAAGA ATAAGAGTAA AAAGCAAGAA GACGAAGGCT CGACTCAAAG CAATGCTTCC 
AGCAACTCAG CATCCGAGGA ATCCAACCGC TCTGGGTCGG AGTCTGGAAG TCAGTCGGAG 
AGCGAGCAGG GCAGTGAAAA AGCACAATCC CGGCATTCTG AATCCAACAG CACGTCTGAT 
TCTGAGAGCC ACTCTGAGTC AGGCAGCGAA TCCAATGGGT CCAAATCTCA TCAGAGCTCA 
GCGCAGGTCA AAGACAAACC TGTCAGGAAG AAAGACAACC TGGCGGATGT GAAGAAGATG 
TGGGAAGAAC ATCCTGATGT TTATGGTGTG AGAAGATCCA GTCGCAGTAG ACAAGAGCCT 
GCACGCCTTA ATATTGGAGC TGAGGGCAGC AGTGATTCAG AGGGAGAAAG TTCCAAACGA 
AAAAGCTCTC GGCAGAAAAA GAAAGAAAAT ACCTGGAAAG ATGATGACTC AAATGACGAA 
GAGGATGTTG AGGAAGAGAC CAGCAGTTCA GAGAGTGAGC AGGAAGAGAA AAAAGTTAGA 
TCCAGACGAC TTCCTGCTAG AAGACCACAG ACCAAATCAT CTGCTAGTAA ACAGTCACAA 
AAGGGACGAA AACAGAGAAA GCAGGAGTCC TCAGATGAAG ATGATGACGA TGATGATGAT 
GACGAGGAGG ACACTCCAAA AAGACAAACA CGGCGGCGGG CTGCTACTAA AGTCAGTTAC 
AAAGAGGACC AGAATGATTT TGAGACGGAC TCTGATGACC TTATTGAAAT GGCAGAAGGG 
GCAGAGGAGC AGCAAGATGA TGACAGTGAG ACCATTGAGA AGGTTGTTGA CACTAGGACT 
GGCAAGAAAG GAGCTACTGG AGCTTCCACA ACTCTCTATG CCATTGAGGA AAACGGGGAC 
CCTGGAGCAG ACTTTGACCC GGAGAAAGAG GAAGGAGAAA CTCAGTTCCT CATCAAGTGG 
AAAGGCTGGT CCTACATCCA CAACACCTGG GAGAGTGTGG ATTCCCTCAC TCAGCAGAAA 
GTCAAAGGCA TGAAGAAGCT GGAAAACTTC AAGAAGAAAA ATGAAGAGCT TAATGCCTGG 
TTGAAAAAAG CATCCCCTGA GGATTTAGAA TATTACAACT GTCAGCAAGA GCTCACAAAC 
GACCTGAGCA AGCAGTTCCA GATAATAGAG AGGGTGATTG CTCATAAAAG CAGCTCTGGT 
GAGCCCGAGT ACCTGTGTAA ATGGATGGGT CTGCCGTACG CTGAGTGCAC ATGGGAGGAC 
AGCGCTTTAA TCAAAAAGAA ATTTCAGGCC TGCATTGACA GTTTCCACAA CAGAAACAGC 
TCCAAGACTG TCCCTTCCAA AGACTGTAAG GTGTTGAAGC AGAGGCCAAG ATTTGTGCCC 
TTAAAGAAGC AGCCATCGTA CATTGGAGAT GAGAATCTTG AACTCAGAGA TTACCAGCTA 
GATGGAGTGA ACTGGCTGGC ACACTCCTGG TGCAGGTGTA ACAGTGTTAT CTTGGCTGAT 
GAAATGGGTT TAGGAAAGAC CATTCAGACG ATCTCCTTCC TCTCCTACCT TTTTCATCAG 
CACCAACTTT ACGGCCCCTT CATCGTGGTG GTGCCCCTGT CGACCCTCAC GTCCTGGCAG 
AGGGAGTTTG ACACTTGGGC CCCGGACATG AATGTGGTGG TGTATCTGGG AGATGTCACA 
AGTAGAAAGA CAATCCGAGA CTATGAATGG ATCAATCCAC AGACAAAAAG GATCAAGTTT 
AATGCACTTT TGACCACATA TGAAATTCTA CTGAAGGACA AGGGAGTGCT GGGGAATATA 
AACTGGGCTT TCCTTGGAGT AGATGAAGCT CACCGGCTTA AGAATGACGA CTCGCTGCTG 
TATAAAACTC TGATCGACTT TCGATCCAAT CACAGGCTGC TTATTACCGG GACTCCTCTG 
CAAAACTCCC TCAAAGAGCT GTGGTCCCTT CTGCACTTTC TGATGTCTGA CAAGTTTGAG 
TCCTGGGAGG ATTTTGAGGA TGAGCATGGT AAAGGCAGGG ATAATGGTTA TCAGAGTTTG 
CACAAAGTTC TAGAGCCTTT CCTGCTACGT CGTGTCAAGA AAGATGTGGA GAAATCCCTG 
CCGGCTAAAG TGGAGCAAAT CCTCAGAGTC GACATGTCAG CCCAGCAAAA ACAGTTCTAC 
AAATGGATCC TAACAAGAAA CTTCAAGGCC TTGTCTAAAG GCACTAGAGG CAGCTCTTCT 
GGCTTTCTGA ACATTGTCAT GGAGCTGAAG AAGTGTTGTA ACCACGCTTT CCTCATCAAA 
CAACCAGAAG ATGGAGAAAA TGATGCTCCA CAAGAGCATC TACAGAGTTT GGTGCGTGGT 
GGAGGGAAAC TGGTTCTCCT AGACAAGCTT CTCACACGAC TGAAGGACAG AGGCAACCGT 
GTGCTTATCT TCTCTCAGAT GGTCCGCATG TTGGACATTC TTGCTGATTA CTTGTCTATG 
AAACGATATC AATTCCAGCG TTTGGACGGA TCCATCAAGG GTGAGCTCCG AAAACAAGCA 
TTGGACCACT TTAATGCAGA AGGCTCTGAG GACTTCTGTT TCCTGCTGTC CACCCGAGCC 
GGAGGGCTTG GGATTAACTT GGCCTCTGCT GACACAGTGG TGATCTTTGA CTCTGACTGG 
AACCCACAGA ATGATCTTCA AGCTCAGGCC AGAGCCCACA GGATTGGCCA GAAAAAGCAA 
GTGAATATCT ATCGTCTGGT CACAAAAGGG ACTGTGGAGG AGGACATTAT TGAAAGAGCT 
AAAAAGAAGA TGGTTCTAGA CCATCTTGTC ATTCAGAGAA TGGACACCAC TGGTCGAACC 
GTACTGGATA ACAGTTCAGC AAATTCAAAT TCAAATCCCT TCAATAAGGA AGAACTGTCA 
GCGATCTTGA AGTTTGGAGC AGAGGATCTG TTTAAGGAAG CTGAGGGGGA GGAATCAGAA 
CCGCAGGAAA TGGACATCGA TGAGATATTG CGGCTTGCTG AAACCAGAGA GAGCGATCAG 
GGATCCAGTG CTACCGATGA GCTTCTGTCT CAGTTTAAGG TGGCTAACTT CACCATGGAT 
GAAAGCACAC CAGATCTTGA TGAAAAACCT GGCCGGGACT GGGATGAAAT AATTCCAGAA 
GAGCAGCGGC GGAAGGTGGA GGAAGAGCAG AAGCAGAAGG AGATGGAGGA TATCTACATG 
CTGCCAAGAA GCAGAAGCTC CAACAAGAAG GCACAAGCCA ATGACAGCGA CAGTGATGTG 
GGATCCAAAC TAAAGCACAG ATCCTCAGGT TCAGACAGTG AAACCGATGA CAGCGAGGAT 
GACAAGCGGC CTAAGAGGCG AGGAAGGCCA AGAGCCCGCA AAAACAACGT GGAGGGATTT 
ACTGATGCTG AGATCCGCAG GTTTATAAAA GCATACAAGA AATTTGGAGC TCCACTTGAG 
AGATTGGAGG CGATTGCCCG TGACTCTGAG CTGGTGGAGA AGTCCATTGC AGACCTGAAG 
CGACTAGGGG AGCTGGTGCA CAACAGCTGC GTGACTGCAG TACAGGAGCA CGAAGAGCAC 
TTGAAGGAAA ACCCTGTGGA AGCTAAAGGC CCAGGGAAAA GACGAGGCAT TAACATAAAA 
ATCTCTGGAG TTCAGGTCAA TGCTAAATCA ATTATCCAGC ATGAAGAGGA GTTTGAGCCC 
CTGCATAAAG CTGTGCCTTC AAATCCTGCT GAAAGGAACA AATTTCAGTT GACCTGCCGG 
GTGAAAACTC CTCACTTTGA TGTGGACTGG GATATTCACG ATGACACACA GTTGTTATTG 
GGAATATATG AGCACGGCTA CGGCAACTGG GACTTGATCA AGACTGATCC TGACCTCAAA 
CTTTCAGAGA AGATTCTTCT TGATGACCCT GAAAAGAAAC CTCAAGGAAA GCAGTTACAG 
ATGCGAACAG ACTACTTGCT GAAGATGCTG AAGAAAGACC TGGAAAGCCA AGATGCCATT 
AAAGCAGGAG ATGAGCCCAA AATTAAAAAG AGAAAACCTC GGATGAAGAA GAAGGCCCCT 
AGCAATGAAC TGGTCAATGA AATCGCTTCT CCTCGTCTAT CCGACAACCC ATCAGAAGAG 
GGAGAGGTAA AGGATGATGG AGCTGAAAAA ACACCCACAA AGAAGAAACA GAAAAAGGAC 
AACAAAGAGA ACAAAGAAAA AACAGGGACT CCTAAAAAAG AGAAGGAGGG GGACAAAGAC 
AAAAAGCGTT CCAAGCCCAA AAAAGAAAAG GCCAAACCAG CAGGAAAAGG GAAGAAAGCC 
CAGGGCCCTG TGCACATCAC TCAAGGAAGT GAACCTATAC CAATTGGAGA GGAAGATGAT 
GAGCTGGACC AGGAAACATT TAGCATTTGT AAGGAGCGGA TGCGGCCAGT GAAGAAAGCT 
CTTAAGCAGT TGGATAAGCC AGACGAGGGG CTGTCGGTGC AGGACCAGCT GCAGCACACA 
CGTACCTGTC TGCTGAAGAT CGGAGACCGC ATTACAGAGT GCCTTAAGTC ATATAGTGAC 
CCCGAGCACA TCAAGATCTG GCGCAGAAAC CTCTGGATAT TTGTGTCCAA ATTTACTGAG 
TTTGGTGCGC AGAAGCTGCA TAGGCTATAT AAGAAGGCTC AGAAGAAGCG CTCCCAGGAG 
GGAGAGAAAG AGCATAAGAA AAAAGACGAT CCTATTAAGA AAAAGCCCTT TAGGCCTGAA 
CCGTCTGGAT CTAGCAGGGA TTCAACGGGT GCCCAGCCTA CAACCAAACC CCATCCCCCT 
GTCAGCCATC AATCTGGACC TCCTCATCCT CCACACCATA GAGAACAGTA CAACCAGCAT 
AATAAGAGGC ATTTTCCTAA TGATGACAGG GGAGATTGGC AAAGGGACCG CAAATATGGC 
TACCCAGGTA ACAGCAATCA GCCCTGGCAA GGAGACCGTC ATCACCCTTA TGAGCCTCAC 
AGGTACAAGG ATCATCACTA CAGTGACCGT CGTCCTCATG GTGACTACCG CAGCTCTGGA 
GGATACCGTA ACAGTGGCTC CCCTCGCAAA CGGCCCTATG ACCAGTATGG CAACGATAGA 
GATCATCGGG GTCATCGAGA CTATTATGAC AGACATCCGG ACCCAAAGAG AAGGCGCTCG 
GATGAATATC GCTCTCCAAA CTACCACCAA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA 
GGAGGAGGGC ATCCGCAGGA CTTTAGAAGA ATGCCTGACC ACAGGGGACC TCCTGGAAAC 
CATGGCCCCA TGGGGCCGGA TCACTACCGG CCCTTTCACC CAGACAAACC CCCGCCTCTG 
ATTGACCCAC GTTCTCCGCA GTCTCAGAAA TCGCCTCTTG AGCCGATCTC ACCTGGTATG 
GACAGAAGTG CAGAGCAGAA ATCTGCGACA GACTTTAACT GGAACAACAG GAAGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd2 ( XM_021467690.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323365.1 Danio rerio chromodomain helicase DNA binding protein 2 (chd2), transcript variant X1, mRNA.

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CloneID	ODa45852
Clone ID Related Accession (Same CDS sequence)	XM_021467690.1 , XM_021467691.1
Accession Version	XM_021467690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chromodomain-helicase-DNA-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA ATAAGAGTAA AAAGCAAGAA GACGAAGGCT CGACTCAAAG CAATGCTTCC 
AGCAACTCAG CATCCGAGGA ATCCAACCGC TCTGGGTCGG AGTCTGGAAG TCAGTCGGAG 
AGCGAGCAGG GCAGTGAAAA AGCACAATCC CGGCATTCTG AATCCAACAG CACGTCTGAT 
TCTGAGAGCC ACTCTGAGTC AGGCAGCGAA TCCAATGGGT CCAAATCTCA TCAGAGCTCA 
GCGCAGGTCA AAGACAAACC TGTCAGGAAG AAAGACAACC TGGCGGATGT GAAGAAGGTA 
CACAAAAAGA TGTGGGAAGA ACATCCTGAT GTTTATGGTG TGAGAAGATC CAGTCGCAGT 
AGACAAGAGC CTGCACGCCT TAATATTGGA GCTGAGGGCA GCAGTGATTC AGAGGGAGAA 
AGTTCCAAAC GAAAAAGCTC TCGGCAGAAA AAGAAAGAAA ATACCTGGAA AGATGATGAC 
TCAAATGACG AAGAGGATGT TGAGGAAGAG ACCAGCAGTT CAGAGAGTGA GCAGGAAGAG 
AAAAAAGTTA GATCCAGACG ACTTCCTGCT AGAAGACCAC AGACCAAATC ATCTGCTAGT 
AAACAGTCAC AAAAGGGACG AAAACAGAGA AAGCAGGAGT CCTCAGATGA AGATGATGAC 
GATGATGATG ATGACGAGGA GGACACTCCA AAAAGACAAA CACGGCGGCG GGCTGCTACT 
AAAGTCAGTT ACAAAGAGGA CCAGAATGAT TTTGAGACGG ACTCTGATGA CCTTATTGAA 
ATGGCAGAAG GGGCAGAGGA GCAGCAAGAT GATGACAGTG AGACCATTGA GAAGGTTGTT 
GACACTAGGA CTGGCAAGAA AGGAGCTACT GGAGCTTCCA CAACTCTCTA TGCCATTGAG 
GAAAACGGGG ACCCTGGAGC AGACTTTGAC CCGGAGAAAG AGGAAGGAGA AACTCAGTTC 
CTCATCAAGT GGAAAGGCTG GTCCTACATC CACAACACCT GGGAGAGTGT GGATTCCCTC 
ACTCAGCAGA AAGTCAAAGG CATGAAGAAG CTGGAAAACT TCAAGAAGAA AAATGAAGAG 
CTTAATGCCT GGTTGAAAAA AGCATCCCCT GAGGATTTAG AATATTACAA CTGTCAGCAA 
GAGCTCACAA ACGACCTGAG CAAGCAGTTC CAGATAATAG AGAGGGTGAT TGCAACAAGA 
ACAGGCAAAT CACAAGCAAC TTCAGACACT CCTTCTCATA AAAGCAGCTC TGGTGAGCCC 
GAGTACCTGT GTAAATGGAT GGGTCTGCCG TACGCTGAGT GCACATGGGA GGACAGCGCT 
TTAATCAAAA AGAAATTTCA GGCCTGCATT GACAGTTTCC ACAACAGAAA CAGCTCCAAG 
ACTGTCCCTT CCAAAGACTG TAAGGTGTTG AAGCAGAGGC CAAGATTTGT GCCCTTAAAG 
AAGCAGCCAT CGTACATTGG AGATGAGAAT CTTGAACTCA GAGATTACCA GCTAGATGGA 
GTGAACTGGC TGGCACACTC CTGGTGCAGG TGTAACAGTG TTATCTTGGC TGATGAAATG 
GGTTTAGGAA AGACCATTCA GACGATCTCC TTCCTCTCCT ACCTTTTTCA TCAGCACCAA 
CTTTACGGCC CCTTCATCGT GGTGGTGCCC CTGTCGACCC TCACGTCCTG GCAGAGGGAG 
TTTGACACTT GGGCCCCGGA CATGAATGTG GTGGTGTATC TGGGAGATGT CACAAGTAGA 
AAGACAATCC GAGACTATGA ATGGATCAAT CCACAGACAA AAAGGATCAA GTTTAATGCA 
CTTTTGACCA CATATGAAAT TCTACTGAAG GACAAGGGAG TGCTGGGGAA TATAAACTGG 
GCTTTCCTTG GAGTAGATGA AGCTCACCGG CTTAAGAATG ACGACTCGCT GCTGTATAAA 
ACTCTGATCG ACTTTCGATC CAATCACAGG CTGCTTATTA CCGGGACTCC TCTGCAAAAC 
TCCCTCAAAG AGCTGTGGTC CCTTCTGCAC TTTCTGATGT CTGACAAGTT TGAGTCCTGG 
GAGGATTTTG AGGATGAGCA TGGTAAAGGC AGGGATAATG GTTATCAGAG TTTGCACAAA 
GTTCTAGAGC CTTTCCTGCT ACGTCGTGTC AAGAAAGATG TGGAGAAATC CCTGCCGGCT 
AAAGTGGAGC AAATCCTCAG AGTCGACATG TCAGCCCAGC AAAAACAGTT CTACAAATGG 
ATCCTAACAA GAAACTTCAA GGCCTTGTCT AAAGGCACTA GAGGCAGCTC TTCTGGCTTT 
CTGAACATTG TCATGGAGCT GAAGAAGTGT TGTAACCACG CTTTCCTCAT CAAACAACCA 
GAAGATGGAG AAAATGATGC TCCACAAGAG CATCTACAGA GTTTGGTGCG TGGTGGAGGG 
AAACTGGTTC TCCTAGACAA GCTTCTCACA CGACTGAAGG ACAGAGGCAA CCGTGTGCTT 
ATCTTCTCTC AGATGGTCCG CATGTTGGAC ATTCTTGCTG ATTACTTGTC TATGAAACGA 
TATCAATTCC AGCGTTTGGA CGGATCCATC AAGGGTGAGC TCCGAAAACA AGCATTGGAC 
CACTTTAATG CAGAAGGCTC TGAGGACTTC TGTTTCCTGC TGTCCACCCG AGCCGGAGGG 
CTTGGGATTA ACTTGGCCTC TGCTGACACA GTGGTGATCT TTGACTCTGA CTGGAACCCA 
CAGAATGATC TTCAAGCTCA GGCCAGAGCC CACAGGATTG GCCAGAAAAA GCAAGTGAAT 
ATCTATCGTC TGGTCACAAA AGGGACTGTG GAGGAGGACA TTATTGAAAG AGCTAAAAAG 
AAGATGGTTC TAGACCATCT TGTCATTCAG AGAATGGACA CCACTGGTCG AACCGTACTG 
GATAACAGTT CAGCAAATTC AAATTCAAAT CCCTTCAATA AGGAAGAACT GTCAGCGATC 
TTGAAGTTTG GAGCAGAGGA TCTGTTTAAG GAAGCTGAGG GGGAGGAATC AGAACCGCAG 
GAAATGGACA TCGATGAGAT ATTGCGGCTT GCTGAAACCA GAGAGAGCGA TCAGGGATCC 
AGTGCTACCG ATGAGCTTCT GTCTCAGTTT AAGGTGGCTA ACTTCACCAT GGATGAAAGC 
ACACCAGATC TTGATGAAAA ACCTGGCCGG GACTGGGATG AAATAATTCC AGAAGAGCAG 
CGGCGGAAGG TGGAGGAAGA GCAGAAGCAG AAGGAGATGG AGGATATCTA CATGCTGCCA 
AGAAGCAGAA GCTCCAACAA GAAGGCACAA GCCAATGACA GCGACAGTGA TGTGGGATCC 
AAACTAAAGC ACAGATCCTC AGGTTCAGAC AGTGAAACCG ATGACAGCGA GGATGACAAG 
CGGCCTAAGA GGCGAGGAAG GCCAAGAGCC CGCAAAAACA ACGTGGAGGG ATTTACTGAT 
GCTGAGATCC GCAGGTTTAT AAAAGCATAC AAGAAATTTG GAGCTCCACT TGAGAGATTG 
GAGGCGATTG CCCGTGACTC TGAGCTGGTG GAGAAGTCCA TTGCAGACCT GAAGCGACTA 
GGGGAGCTGG TGCACAACAG CTGCGTGACT GCAGTACAGG AGCACGAAGA GCACTTGAAG 
GAAAACCCTG TGGAAGCTAA AGGCCCAGGG AAAAGACGAG GCATTAACAT AAAAATCTCT 
GGAGTTCAGG TCAATGCTAA ATCAATTATC CAGCATGAAG AGGAGTTTGA GCCCCTGCAT 
AAAGCTGTGC CTTCAAATCC TGCTGAAAGG AACAAATTTC AGTTGACCTG CCGGGTGAAA 
ACTCCTCACT TTGATGTGGA CTGGGATATT CACGATGACA CACAGTTGTT ATTGGGAATA 
TATGAGCACG GCTACGGCAA CTGGGACTTG ATCAAGACTG ATCCTGACCT CAAACTTTCA 
GAGAAGATTC TTCTTGATGA CCCTGAAAAG AAACCTCAAG GAAAGCAGTT ACAGATGCGA 
ACAGACTACT TGCTGAAGAT GCTGAAGAAA GACCTGGAAA GCCAAGATGC CATTAAAGCA 
GGAGATGAGC CCAAAATTAA AAAGAGAAAA CCTCGGATGA AGAAGAAGGC CCCTAGCAAT 
GAACTGGTCA ATGAAATCGC TTCTCCTCGT CTATCCGACA ACCCATCAGA AGAGGGAGAG 
GTAAAGGATG ATGGAGCTGA AAAAACACCC ACAAAGAAGA AACAGAAAAA GGACAACAAA 
GAGAACAAAG AAAAAACAGG GACTCCTAAA AAAGAGAAGG AGGGGGACAA AGACAAAAAG 
CGTTCCAAGC CCAAAAAAGA AAAGGCCAAA CCAGCAGGAA AAGGGAAGAA AGCCCAGGGC 
CCTGTGCACA TCACTCAAGG AAGTGAACCT ATACCAATTG GAGAGGAAGA TGATGAGCTG 
GACCAGGAAA CATTTAGCAT TTGTAAGGAG CGGATGCGGC CAGTGAAGAA AGCTCTTAAG 
CAGTTGGATA AGCCAGACGA GGGGCTGTCG GTGCAGGACC AGCTGCAGCA CACACGTACC 
TGTCTGCTGA AGATCGGAGA CCGCATTACA GAGTGCCTTA AGTCATATAG TGACCCCGAG 
CACATCAAGA TCTGGCGCAG AAACCTCTGG ATATTTGTGT CCAAATTTAC TGAGTTTGGT 
GCGCAGAAGC TGCATAGGCT ATATAAGAAG GCTCAGAAGA AGCGCTCCCA GGAGGGAGAG 
AAAGAGCATA AGAAAAAAGA CGATCCTATT AAGAAAAAGC CCTTTAGGCC TGAACCGTCT 
GGATCTAGCA GGGATTCAAC GGGTGCCCAG CCTACAACCA AACCCCATCC CCCTGTCAGC 
CATCAATCTG GACCTCCTCA TCCTCCACAC CATAGAGAAC AGTACAACCA GCATAATAAG 
AGGCATTTTC CTAATGATGA CAGGGGAGAT TGGCAAAGGG ACCGCAAATA TGGCTACCCA 
GGTAACAGCA ATCAGCCCTG GCAAGGAGAC CGTCATCACC CTTATGAGCC TCACAGGTAC 
AAGGATCATC ACTACAGTGA CCGTCGTCCT CATGGTGACT ACCGCAGCTC TGGAGGATAC 
CGTAACAGTG GCTCCCCTCG CAAACGGCCC TATGACCAGT ATGGCAACGA TAGAGATCAT 
CGGGGTCATC GAGACTATTA TGACAGACAT CCGGACCCAA AGAGAAGGCG CTCGGATGAA 
TATCGCTCTC CAAACTACCA CCAAGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA 
GGGCATCCGC AGGACTTTAG AAGAATGCCT GACCACAGGG GACCTCCTGG AAACCATGGC 
CCCATGGGGC CGGATCACTA CCGGCCCTTT CACCCAGACA AACCCCCGCC TCTGATTGAC 
CCACGTTCTC CGCAGTCTCA GAAATCGCCT CTTGAGCCGA TCTCACCTGG TATGGACAGA 
AGTGCAGAGC AGAAATCTGC GACAGACTTT AACTGGAACA ACAGGAAGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323365.1
CDS	1941..7394
Translation	MMKNKSKKQEDEGSTQSNASSNSASEESNRSGSESGSQSESEQGSEKAQSRHSESNSTSDSESHSESGSESNGSKSHQSSAQVKDKPVRKKDNLADVKKVHKKMWEEHPDVYGVRRSSRSRQEPARLNIGAEGSSDSEGESSKRKSSRQKKKENTWKDDDSNDEEDVEEETSSSESEQEEKKVRSRRLPARRPQTKSSASKQSQKGRKQRKQESSDEDDDDDDDDEEDTPKRQTRRRAATKVSYKEDQNDFETDSDDLIEMAEGAEEQQDDDSETIEKVVDTRTGKKGATGASTTLYAIEENGDPGADFDPEKEEGETQFLIKWKGWSYIHNTWESVDSLTQQKVKGMKKLENFKKKNEELNAWLKKASPEDLEYYNCQQELTNDLSKQFQIIERVIATRTGKSQATSDTPSHKSSSGEPEYLCKWMGLPYAECTWEDSALIKKKFQACIDSFHNRNSSKTVPSKDCKVLKQRPRFVPLKKQPSYIGDENLELRDYQLDGVNWLAHSWCRCNSVILADEMGLGKTIQTISFLSYLFHQHQLYGPFIVVVPLSTLTSWQREFDTWAPDMNVVVYLGDVTSRKTIRDYEWINPQTKRIKFNALLTTYEILLKDKGVLGNINWAFLGVDEAHRLKNDDSLLYKTLIDFRSNHRLLITGTPLQNSLKELWSLLHFLMSDKFESWEDFEDEHGKGRDNGYQSLHKVLEPFLLRRVKKDVEKSLPAKVEQILRVDMSAQQKQFYKWILTRNFKALSKGTRGSSSGFLNIVMELKKCCNHAFLIKQPEDGENDAPQEHLQSLVRGGGKLVLLDKLLTRLKDRGNRVLIFSQMVRMLDILADYLSMKRYQFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGTVEEDIIERAKKKMVLDHLVIQRMDTTGRTVLDNSSANSNSNPFNKEELSAILKFGAEDLFKEAEGEESEPQEMDIDEILRLAETRESDQGSSATDELLSQFKVANFTMDESTPDLDEKPGRDWDEIIPEEQRRKVEEEQKQKEMEDIYMLPRSRSSNKKAQANDSDSDVGSKLKHRSSGSDSETDDSEDDKRPKRRGRPRARKNNVEGFTDAEIRRFIKAYKKFGAPLERLEAIARDSELVEKSIADLKRLGELVHNSCVTAVQEHEEHLKENPVEAKGPGKRRGINIKISGVQVNAKSIIQHEEEFEPLHKAVPSNPAERNKFQLTCRVKTPHFDVDWDIHDDTQLLLGIYEHGYGNWDLIKTDPDLKLSEKILLDDPEKKPQGKQLQMRTDYLLKMLKKDLESQDAIKAGDEPKIKKRKPRMKKKAPSNELVNEIASPRLSDNPSEEGEVKDDGAEKTPTKKKQKKDNKENKEKTGTPKKEKEGDKDKKRSKPKKEKAKPAGKGKKAQGPVHITQGSEPIPIGEEDDELDQETFSICKERMRPVKKALKQLDKPDEGLSVQDQLQHTRTCLLKIGDRITECLKSYSDPEHIKIWRRNLWIFVSKFTEFGAQKLHRLYKKAQKKRSQEGEKEHKKKDDPIKKKPFRPEPSGSSRDSTGAQPTTKPHPPVSHQSGPPHPPHHREQYNQHNKRHFPNDDRGDWQRDRKYGYPGNSNQPWQGDRHHPYEPHRYKDHHYSDRRPHGDYRSSGGYRNSGSPRKRPYDQYGNDRDHRGHRDYYDRHPDPKRRRSDEYRSPNYHQGGGGGGGGGGGGGHPQDFRRMPDHRGPPGNHGPMGPDHYRPFHPDKPPPLIDPRSPQSQKSPLEPISPGMDRSAEQKSATDFNWNNRKT

Target ORF information:

RefSeq Version	XM_021467690.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 2 (chd2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467690.1

ORF Insert Sequence:

ATGATGAAGA ATAAGAGTAA AAAGCAAGAA GACGAAGGCT CGACTCAAAG CAATGCTTCC 
AGCAACTCAG CATCCGAGGA ATCCAACCGC TCTGGGTCGG AGTCTGGAAG TCAGTCGGAG 
AGCGAGCAGG GCAGTGAAAA AGCACAATCC CGGCATTCTG AATCCAACAG CACGTCTGAT 
TCTGAGAGCC ACTCTGAGTC AGGCAGCGAA TCCAATGGGT CCAAATCTCA TCAGAGCTCA 
GCGCAGGTCA AAGACAAACC TGTCAGGAAG AAAGACAACC TGGCGGATGT GAAGAAGGTA 
CACAAAAAGA TGTGGGAAGA ACATCCTGAT GTTTATGGTG TGAGAAGATC CAGTCGCAGT 
AGACAAGAGC CTGCACGCCT TAATATTGGA GCTGAGGGCA GCAGTGATTC AGAGGGAGAA 
AGTTCCAAAC GAAAAAGCTC TCGGCAGAAA AAGAAAGAAA ATACCTGGAA AGATGATGAC 
TCAAATGACG AAGAGGATGT TGAGGAAGAG ACCAGCAGTT CAGAGAGTGA GCAGGAAGAG 
AAAAAAGTTA GATCCAGACG ACTTCCTGCT AGAAGACCAC AGACCAAATC ATCTGCTAGT 
AAACAGTCAC AAAAGGGACG AAAACAGAGA AAGCAGGAGT CCTCAGATGA AGATGATGAC 
GATGATGATG ATGACGAGGA GGACACTCCA AAAAGACAAA CACGGCGGCG GGCTGCTACT 
AAAGTCAGTT ACAAAGAGGA CCAGAATGAT TTTGAGACGG ACTCTGATGA CCTTATTGAA 
ATGGCAGAAG GGGCAGAGGA GCAGCAAGAT GATGACAGTG AGACCATTGA GAAGGTTGTT 
GACACTAGGA CTGGCAAGAA AGGAGCTACT GGAGCTTCCA CAACTCTCTA TGCCATTGAG 
GAAAACGGGG ACCCTGGAGC AGACTTTGAC CCGGAGAAAG AGGAAGGAGA AACTCAGTTC 
CTCATCAAGT GGAAAGGCTG GTCCTACATC CACAACACCT GGGAGAGTGT GGATTCCCTC 
ACTCAGCAGA AAGTCAAAGG CATGAAGAAG CTGGAAAACT TCAAGAAGAA AAATGAAGAG 
CTTAATGCCT GGTTGAAAAA AGCATCCCCT GAGGATTTAG AATATTACAA CTGTCAGCAA 
GAGCTCACAA ACGACCTGAG CAAGCAGTTC CAGATAATAG AGAGGGTGAT TGCAACAAGA 
ACAGGCAAAT CACAAGCAAC TTCAGACACT CCTTCTCATA AAAGCAGCTC TGGTGAGCCC 
GAGTACCTGT GTAAATGGAT GGGTCTGCCG TACGCTGAGT GCACATGGGA GGACAGCGCT 
TTAATCAAAA AGAAATTTCA GGCCTGCATT GACAGTTTCC ACAACAGAAA CAGCTCCAAG 
ACTGTCCCTT CCAAAGACTG TAAGGTGTTG AAGCAGAGGC CAAGATTTGT GCCCTTAAAG 
AAGCAGCCAT CGTACATTGG AGATGAGAAT CTTGAACTCA GAGATTACCA GCTAGATGGA 
GTGAACTGGC TGGCACACTC CTGGTGCAGG TGTAACAGTG TTATCTTGGC TGATGAAATG 
GGTTTAGGAA AGACCATTCA GACGATCTCC TTCCTCTCCT ACCTTTTTCA TCAGCACCAA 
CTTTACGGCC CCTTCATCGT GGTGGTGCCC CTGTCGACCC TCACGTCCTG GCAGAGGGAG 
TTTGACACTT GGGCCCCGGA CATGAATGTG GTGGTGTATC TGGGAGATGT CACAAGTAGA 
AAGACAATCC GAGACTATGA ATGGATCAAT CCACAGACAA AAAGGATCAA GTTTAATGCA 
CTTTTGACCA CATATGAAAT TCTACTGAAG GACAAGGGAG TGCTGGGGAA TATAAACTGG 
GCTTTCCTTG GAGTAGATGA AGCTCACCGG CTTAAGAATG ACGACTCGCT GCTGTATAAA 
ACTCTGATCG ACTTTCGATC CAATCACAGG CTGCTTATTA CCGGGACTCC TCTGCAAAAC 
TCCCTCAAAG AGCTGTGGTC CCTTCTGCAC TTTCTGATGT CTGACAAGTT TGAGTCCTGG 
GAGGATTTTG AGGATGAGCA TGGTAAAGGC AGGGATAATG GTTATCAGAG TTTGCACAAA 
GTTCTAGAGC CTTTCCTGCT ACGTCGTGTC AAGAAAGATG TGGAGAAATC CCTGCCGGCT 
AAAGTGGAGC AAATCCTCAG AGTCGACATG TCAGCCCAGC AAAAACAGTT CTACAAATGG 
ATCCTAACAA GAAACTTCAA GGCCTTGTCT AAAGGCACTA GAGGCAGCTC TTCTGGCTTT 
CTGAACATTG TCATGGAGCT GAAGAAGTGT TGTAACCACG CTTTCCTCAT CAAACAACCA 
GAAGATGGAG AAAATGATGC TCCACAAGAG CATCTACAGA GTTTGGTGCG TGGTGGAGGG 
AAACTGGTTC TCCTAGACAA GCTTCTCACA CGACTGAAGG ACAGAGGCAA CCGTGTGCTT 
ATCTTCTCTC AGATGGTCCG CATGTTGGAC ATTCTTGCTG ATTACTTGTC TATGAAACGA 
TATCAATTCC AGCGTTTGGA CGGATCCATC AAGGGTGAGC TCCGAAAACA AGCATTGGAC 
CACTTTAATG CAGAAGGCTC TGAGGACTTC TGTTTCCTGC TGTCCACCCG AGCCGGAGGG 
CTTGGGATTA ACTTGGCCTC TGCTGACACA GTGGTGATCT TTGACTCTGA CTGGAACCCA 
CAGAATGATC TTCAAGCTCA GGCCAGAGCC CACAGGATTG GCCAGAAAAA GCAAGTGAAT 
ATCTATCGTC TGGTCACAAA AGGGACTGTG GAGGAGGACA TTATTGAAAG AGCTAAAAAG 
AAGATGGTTC TAGACCATCT TGTCATTCAG AGAATGGACA CCACTGGTCG AACCGTACTG 
GATAACAGTT CAGCAAATTC AAATTCAAAT CCCTTCAATA AGGAAGAACT GTCAGCGATC 
TTGAAGTTTG GAGCAGAGGA TCTGTTTAAG GAAGCTGAGG GGGAGGAATC AGAACCGCAG 
GAAATGGACA TCGATGAGAT ATTGCGGCTT GCTGAAACCA GAGAGAGCGA TCAGGGATCC 
AGTGCTACCG ATGAGCTTCT GTCTCAGTTT AAGGTGGCTA ACTTCACCAT GGATGAAAGC 
ACACCAGATC TTGATGAAAA ACCTGGCCGG GACTGGGATG AAATAATTCC AGAAGAGCAG 
CGGCGGAAGG TGGAGGAAGA GCAGAAGCAG AAGGAGATGG AGGATATCTA CATGCTGCCA 
AGAAGCAGAA GCTCCAACAA GAAGGCACAA GCCAATGACA GCGACAGTGA TGTGGGATCC 
AAACTAAAGC ACAGATCCTC AGGTTCAGAC AGTGAAACCG ATGACAGCGA GGATGACAAG 
CGGCCTAAGA GGCGAGGAAG GCCAAGAGCC CGCAAAAACA ACGTGGAGGG ATTTACTGAT 
GCTGAGATCC GCAGGTTTAT AAAAGCATAC AAGAAATTTG GAGCTCCACT TGAGAGATTG 
GAGGCGATTG CCCGTGACTC TGAGCTGGTG GAGAAGTCCA TTGCAGACCT GAAGCGACTA 
GGGGAGCTGG TGCACAACAG CTGCGTGACT GCAGTACAGG AGCACGAAGA GCACTTGAAG 
GAAAACCCTG TGGAAGCTAA AGGCCCAGGG AAAAGACGAG GCATTAACAT AAAAATCTCT 
GGAGTTCAGG TCAATGCTAA ATCAATTATC CAGCATGAAG AGGAGTTTGA GCCCCTGCAT 
AAAGCTGTGC CTTCAAATCC TGCTGAAAGG AACAAATTTC AGTTGACCTG CCGGGTGAAA 
ACTCCTCACT TTGATGTGGA CTGGGATATT CACGATGACA CACAGTTGTT ATTGGGAATA 
TATGAGCACG GCTACGGCAA CTGGGACTTG ATCAAGACTG ATCCTGACCT CAAACTTTCA 
GAGAAGATTC TTCTTGATGA CCCTGAAAAG AAACCTCAAG GAAAGCAGTT ACAGATGCGA 
ACAGACTACT TGCTGAAGAT GCTGAAGAAA GACCTGGAAA GCCAAGATGC CATTAAAGCA 
GGAGATGAGC CCAAAATTAA AAAGAGAAAA CCTCGGATGA AGAAGAAGGC CCCTAGCAAT 
GAACTGGTCA ATGAAATCGC TTCTCCTCGT CTATCCGACA ACCCATCAGA AGAGGGAGAG 
GTAAAGGATG ATGGAGCTGA AAAAACACCC ACAAAGAAGA AACAGAAAAA GGACAACAAA 
GAGAACAAAG AAAAAACAGG GACTCCTAAA AAAGAGAAGG AGGGGGACAA AGACAAAAAG 
CGTTCCAAGC CCAAAAAAGA AAAGGCCAAA CCAGCAGGAA AAGGGAAGAA AGCCCAGGGC 
CCTGTGCACA TCACTCAAGG AAGTGAACCT ATACCAATTG GAGAGGAAGA TGATGAGCTG 
GACCAGGAAA CATTTAGCAT TTGTAAGGAG CGGATGCGGC CAGTGAAGAA AGCTCTTAAG 
CAGTTGGATA AGCCAGACGA GGGGCTGTCG GTGCAGGACC AGCTGCAGCA CACACGTACC 
TGTCTGCTGA AGATCGGAGA CCGCATTACA GAGTGCCTTA AGTCATATAG TGACCCCGAG 
CACATCAAGA TCTGGCGCAG AAACCTCTGG ATATTTGTGT CCAAATTTAC TGAGTTTGGT 
GCGCAGAAGC TGCATAGGCT ATATAAGAAG GCTCAGAAGA AGCGCTCCCA GGAGGGAGAG 
AAAGAGCATA AGAAAAAAGA CGATCCTATT AAGAAAAAGC CCTTTAGGCC TGAACCGTCT 
GGATCTAGCA GGGATTCAAC GGGTGCCCAG CCTACAACCA AACCCCATCC CCCTGTCAGC 
CATCAATCTG GACCTCCTCA TCCTCCACAC CATAGAGAAC AGTACAACCA GCATAATAAG 
AGGCATTTTC CTAATGATGA CAGGGGAGAT TGGCAAAGGG ACCGCAAATA TGGCTACCCA 
GGTAACAGCA ATCAGCCCTG GCAAGGAGAC CGTCATCACC CTTATGAGCC TCACAGGTAC 
AAGGATCATC ACTACAGTGA CCGTCGTCCT CATGGTGACT ACCGCAGCTC TGGAGGATAC 
CGTAACAGTG GCTCCCCTCG CAAACGGCCC TATGACCAGT ATGGCAACGA TAGAGATCAT 
CGGGGTCATC GAGACTATTA TGACAGACAT CCGGACCCAA AGAGAAGGCG CTCGGATGAA 
TATCGCTCTC CAAACTACCA CCAAGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA 
GGGCATCCGC AGGACTTTAG AAGAATGCCT GACCACAGGG GACCTCCTGG AAACCATGGC 
CCCATGGGGC CGGATCACTA CCGGCCCTTT CACCCAGACA AACCCCCGCC TCTGATTGAC 
CCACGTTCTC CGCAGTCTCA GAAATCGCCT CTTGAGCCGA TCTCACCTGG TATGGACAGA 
AGTGCAGAGC AGAAATCTGC GACAGACTTT AACTGGAACA ACAGGAAGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd2 ( XM_021467691.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323366.1 Danio rerio chromodomain helicase DNA binding protein 2 (chd2), transcript variant X2, mRNA.

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CloneID	ODa45852
Clone ID Related Accession (Same CDS sequence)	XM_021467690.1 , XM_021467691.1
Accession Version	XM_021467691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chromodomain-helicase-DNA-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA ATAAGAGTAA AAAGCAAGAA GACGAAGGCT CGACTCAAAG CAATGCTTCC 
AGCAACTCAG CATCCGAGGA ATCCAACCGC TCTGGGTCGG AGTCTGGAAG TCAGTCGGAG 
AGCGAGCAGG GCAGTGAAAA AGCACAATCC CGGCATTCTG AATCCAACAG CACGTCTGAT 
TCTGAGAGCC ACTCTGAGTC AGGCAGCGAA TCCAATGGGT CCAAATCTCA TCAGAGCTCA 
GCGCAGGTCA AAGACAAACC TGTCAGGAAG AAAGACAACC TGGCGGATGT GAAGAAGGTA 
CACAAAAAGA TGTGGGAAGA ACATCCTGAT GTTTATGGTG TGAGAAGATC CAGTCGCAGT 
AGACAAGAGC CTGCACGCCT TAATATTGGA GCTGAGGGCA GCAGTGATTC AGAGGGAGAA 
AGTTCCAAAC GAAAAAGCTC TCGGCAGAAA AAGAAAGAAA ATACCTGGAA AGATGATGAC 
TCAAATGACG AAGAGGATGT TGAGGAAGAG ACCAGCAGTT CAGAGAGTGA GCAGGAAGAG 
AAAAAAGTTA GATCCAGACG ACTTCCTGCT AGAAGACCAC AGACCAAATC ATCTGCTAGT 
AAACAGTCAC AAAAGGGACG AAAACAGAGA AAGCAGGAGT CCTCAGATGA AGATGATGAC 
GATGATGATG ATGACGAGGA GGACACTCCA AAAAGACAAA CACGGCGGCG GGCTGCTACT 
AAAGTCAGTT ACAAAGAGGA CCAGAATGAT TTTGAGACGG ACTCTGATGA CCTTATTGAA 
ATGGCAGAAG GGGCAGAGGA GCAGCAAGAT GATGACAGTG AGACCATTGA GAAGGTTGTT 
GACACTAGGA CTGGCAAGAA AGGAGCTACT GGAGCTTCCA CAACTCTCTA TGCCATTGAG 
GAAAACGGGG ACCCTGGAGC AGACTTTGAC CCGGAGAAAG AGGAAGGAGA AACTCAGTTC 
CTCATCAAGT GGAAAGGCTG GTCCTACATC CACAACACCT GGGAGAGTGT GGATTCCCTC 
ACTCAGCAGA AAGTCAAAGG CATGAAGAAG CTGGAAAACT TCAAGAAGAA AAATGAAGAG 
CTTAATGCCT GGTTGAAAAA AGCATCCCCT GAGGATTTAG AATATTACAA CTGTCAGCAA 
GAGCTCACAA ACGACCTGAG CAAGCAGTTC CAGATAATAG AGAGGGTGAT TGCAACAAGA 
ACAGGCAAAT CACAAGCAAC TTCAGACACT CCTTCTCATA AAAGCAGCTC TGGTGAGCCC 
GAGTACCTGT GTAAATGGAT GGGTCTGCCG TACGCTGAGT GCACATGGGA GGACAGCGCT 
TTAATCAAAA AGAAATTTCA GGCCTGCATT GACAGTTTCC ACAACAGAAA CAGCTCCAAG 
ACTGTCCCTT CCAAAGACTG TAAGGTGTTG AAGCAGAGGC CAAGATTTGT GCCCTTAAAG 
AAGCAGCCAT CGTACATTGG AGATGAGAAT CTTGAACTCA GAGATTACCA GCTAGATGGA 
GTGAACTGGC TGGCACACTC CTGGTGCAGG TGTAACAGTG TTATCTTGGC TGATGAAATG 
GGTTTAGGAA AGACCATTCA GACGATCTCC TTCCTCTCCT ACCTTTTTCA TCAGCACCAA 
CTTTACGGCC CCTTCATCGT GGTGGTGCCC CTGTCGACCC TCACGTCCTG GCAGAGGGAG 
TTTGACACTT GGGCCCCGGA CATGAATGTG GTGGTGTATC TGGGAGATGT CACAAGTAGA 
AAGACAATCC GAGACTATGA ATGGATCAAT CCACAGACAA AAAGGATCAA GTTTAATGCA 
CTTTTGACCA CATATGAAAT TCTACTGAAG GACAAGGGAG TGCTGGGGAA TATAAACTGG 
GCTTTCCTTG GAGTAGATGA AGCTCACCGG CTTAAGAATG ACGACTCGCT GCTGTATAAA 
ACTCTGATCG ACTTTCGATC CAATCACAGG CTGCTTATTA CCGGGACTCC TCTGCAAAAC 
TCCCTCAAAG AGCTGTGGTC CCTTCTGCAC TTTCTGATGT CTGACAAGTT TGAGTCCTGG 
GAGGATTTTG AGGATGAGCA TGGTAAAGGC AGGGATAATG GTTATCAGAG TTTGCACAAA 
GTTCTAGAGC CTTTCCTGCT ACGTCGTGTC AAGAAAGATG TGGAGAAATC CCTGCCGGCT 
AAAGTGGAGC AAATCCTCAG AGTCGACATG TCAGCCCAGC AAAAACAGTT CTACAAATGG 
ATCCTAACAA GAAACTTCAA GGCCTTGTCT AAAGGCACTA GAGGCAGCTC TTCTGGCTTT 
CTGAACATTG TCATGGAGCT GAAGAAGTGT TGTAACCACG CTTTCCTCAT CAAACAACCA 
GAAGATGGAG AAAATGATGC TCCACAAGAG CATCTACAGA GTTTGGTGCG TGGTGGAGGG 
AAACTGGTTC TCCTAGACAA GCTTCTCACA CGACTGAAGG ACAGAGGCAA CCGTGTGCTT 
ATCTTCTCTC AGATGGTCCG CATGTTGGAC ATTCTTGCTG ATTACTTGTC TATGAAACGA 
TATCAATTCC AGCGTTTGGA CGGATCCATC AAGGGTGAGC TCCGAAAACA AGCATTGGAC 
CACTTTAATG CAGAAGGCTC TGAGGACTTC TGTTTCCTGC TGTCCACCCG AGCCGGAGGG 
CTTGGGATTA ACTTGGCCTC TGCTGACACA GTGGTGATCT TTGACTCTGA CTGGAACCCA 
CAGAATGATC TTCAAGCTCA GGCCAGAGCC CACAGGATTG GCCAGAAAAA GCAAGTGAAT 
ATCTATCGTC TGGTCACAAA AGGGACTGTG GAGGAGGACA TTATTGAAAG AGCTAAAAAG 
AAGATGGTTC TAGACCATCT TGTCATTCAG AGAATGGACA CCACTGGTCG AACCGTACTG 
GATAACAGTT CAGCAAATTC AAATTCAAAT CCCTTCAATA AGGAAGAACT GTCAGCGATC 
TTGAAGTTTG GAGCAGAGGA TCTGTTTAAG GAAGCTGAGG GGGAGGAATC AGAACCGCAG 
GAAATGGACA TCGATGAGAT ATTGCGGCTT GCTGAAACCA GAGAGAGCGA TCAGGGATCC 
AGTGCTACCG ATGAGCTTCT GTCTCAGTTT AAGGTGGCTA ACTTCACCAT GGATGAAAGC 
ACACCAGATC TTGATGAAAA ACCTGGCCGG GACTGGGATG AAATAATTCC AGAAGAGCAG 
CGGCGGAAGG TGGAGGAAGA GCAGAAGCAG AAGGAGATGG AGGATATCTA CATGCTGCCA 
AGAAGCAGAA GCTCCAACAA GAAGGCACAA GCCAATGACA GCGACAGTGA TGTGGGATCC 
AAACTAAAGC ACAGATCCTC AGGTTCAGAC AGTGAAACCG ATGACAGCGA GGATGACAAG 
CGGCCTAAGA GGCGAGGAAG GCCAAGAGCC CGCAAAAACA ACGTGGAGGG ATTTACTGAT 
GCTGAGATCC GCAGGTTTAT AAAAGCATAC AAGAAATTTG GAGCTCCACT TGAGAGATTG 
GAGGCGATTG CCCGTGACTC TGAGCTGGTG GAGAAGTCCA TTGCAGACCT GAAGCGACTA 
GGGGAGCTGG TGCACAACAG CTGCGTGACT GCAGTACAGG AGCACGAAGA GCACTTGAAG 
GAAAACCCTG TGGAAGCTAA AGGCCCAGGG AAAAGACGAG GCATTAACAT AAAAATCTCT 
GGAGTTCAGG TCAATGCTAA ATCAATTATC CAGCATGAAG AGGAGTTTGA GCCCCTGCAT 
AAAGCTGTGC CTTCAAATCC TGCTGAAAGG AACAAATTTC AGTTGACCTG CCGGGTGAAA 
ACTCCTCACT TTGATGTGGA CTGGGATATT CACGATGACA CACAGTTGTT ATTGGGAATA 
TATGAGCACG GCTACGGCAA CTGGGACTTG ATCAAGACTG ATCCTGACCT CAAACTTTCA 
GAGAAGATTC TTCTTGATGA CCCTGAAAAG AAACCTCAAG GAAAGCAGTT ACAGATGCGA 
ACAGACTACT TGCTGAAGAT GCTGAAGAAA GACCTGGAAA GCCAAGATGC CATTAAAGCA 
GGAGATGAGC CCAAAATTAA AAAGAGAAAA CCTCGGATGA AGAAGAAGGC CCCTAGCAAT 
GAACTGGTCA ATGAAATCGC TTCTCCTCGT CTATCCGACA ACCCATCAGA AGAGGGAGAG 
GTAAAGGATG ATGGAGCTGA AAAAACACCC ACAAAGAAGA AACAGAAAAA GGACAACAAA 
GAGAACAAAG AAAAAACAGG GACTCCTAAA AAAGAGAAGG AGGGGGACAA AGACAAAAAG 
CGTTCCAAGC CCAAAAAAGA AAAGGCCAAA CCAGCAGGAA AAGGGAAGAA AGCCCAGGGC 
CCTGTGCACA TCACTCAAGG AAGTGAACCT ATACCAATTG GAGAGGAAGA TGATGAGCTG 
GACCAGGAAA CATTTAGCAT TTGTAAGGAG CGGATGCGGC CAGTGAAGAA AGCTCTTAAG 
CAGTTGGATA AGCCAGACGA GGGGCTGTCG GTGCAGGACC AGCTGCAGCA CACACGTACC 
TGTCTGCTGA AGATCGGAGA CCGCATTACA GAGTGCCTTA AGTCATATAG TGACCCCGAG 
CACATCAAGA TCTGGCGCAG AAACCTCTGG ATATTTGTGT CCAAATTTAC TGAGTTTGGT 
GCGCAGAAGC TGCATAGGCT ATATAAGAAG GCTCAGAAGA AGCGCTCCCA GGAGGGAGAG 
AAAGAGCATA AGAAAAAAGA CGATCCTATT AAGAAAAAGC CCTTTAGGCC TGAACCGTCT 
GGATCTAGCA GGGATTCAAC GGGTGCCCAG CCTACAACCA AACCCCATCC CCCTGTCAGC 
CATCAATCTG GACCTCCTCA TCCTCCACAC CATAGAGAAC AGTACAACCA GCATAATAAG 
AGGCATTTTC CTAATGATGA CAGGGGAGAT TGGCAAAGGG ACCGCAAATA TGGCTACCCA 
GGTAACAGCA ATCAGCCCTG GCAAGGAGAC CGTCATCACC CTTATGAGCC TCACAGGTAC 
AAGGATCATC ACTACAGTGA CCGTCGTCCT CATGGTGACT ACCGCAGCTC TGGAGGATAC 
CGTAACAGTG GCTCCCCTCG CAAACGGCCC TATGACCAGT ATGGCAACGA TAGAGATCAT 
CGGGGTCATC GAGACTATTA TGACAGACAT CCGGACCCAA AGAGAAGGCG CTCGGATGAA 
TATCGCTCTC CAAACTACCA CCAAGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA 
GGGCATCCGC AGGACTTTAG AAGAATGCCT GACCACAGGG GACCTCCTGG AAACCATGGC 
CCCATGGGGC CGGATCACTA CCGGCCCTTT CACCCAGACA AACCCCCGCC TCTGATTGAC 
CCACGTTCTC CGCAGTCTCA GAAATCGCCT CTTGAGCCGA TCTCACCTGG TATGGACAGA 
AGTGCAGAGC AGAAATCTGC GACAGACTTT AACTGGAACA ACAGGAAGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323366.1
CDS	1914..7367
Translation	MMKNKSKKQEDEGSTQSNASSNSASEESNRSGSESGSQSESEQGSEKAQSRHSESNSTSDSESHSESGSESNGSKSHQSSAQVKDKPVRKKDNLADVKKVHKKMWEEHPDVYGVRRSSRSRQEPARLNIGAEGSSDSEGESSKRKSSRQKKKENTWKDDDSNDEEDVEEETSSSESEQEEKKVRSRRLPARRPQTKSSASKQSQKGRKQRKQESSDEDDDDDDDDEEDTPKRQTRRRAATKVSYKEDQNDFETDSDDLIEMAEGAEEQQDDDSETIEKVVDTRTGKKGATGASTTLYAIEENGDPGADFDPEKEEGETQFLIKWKGWSYIHNTWESVDSLTQQKVKGMKKLENFKKKNEELNAWLKKASPEDLEYYNCQQELTNDLSKQFQIIERVIATRTGKSQATSDTPSHKSSSGEPEYLCKWMGLPYAECTWEDSALIKKKFQACIDSFHNRNSSKTVPSKDCKVLKQRPRFVPLKKQPSYIGDENLELRDYQLDGVNWLAHSWCRCNSVILADEMGLGKTIQTISFLSYLFHQHQLYGPFIVVVPLSTLTSWQREFDTWAPDMNVVVYLGDVTSRKTIRDYEWINPQTKRIKFNALLTTYEILLKDKGVLGNINWAFLGVDEAHRLKNDDSLLYKTLIDFRSNHRLLITGTPLQNSLKELWSLLHFLMSDKFESWEDFEDEHGKGRDNGYQSLHKVLEPFLLRRVKKDVEKSLPAKVEQILRVDMSAQQKQFYKWILTRNFKALSKGTRGSSSGFLNIVMELKKCCNHAFLIKQPEDGENDAPQEHLQSLVRGGGKLVLLDKLLTRLKDRGNRVLIFSQMVRMLDILADYLSMKRYQFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGTVEEDIIERAKKKMVLDHLVIQRMDTTGRTVLDNSSANSNSNPFNKEELSAILKFGAEDLFKEAEGEESEPQEMDIDEILRLAETRESDQGSSATDELLSQFKVANFTMDESTPDLDEKPGRDWDEIIPEEQRRKVEEEQKQKEMEDIYMLPRSRSSNKKAQANDSDSDVGSKLKHRSSGSDSETDDSEDDKRPKRRGRPRARKNNVEGFTDAEIRRFIKAYKKFGAPLERLEAIARDSELVEKSIADLKRLGELVHNSCVTAVQEHEEHLKENPVEAKGPGKRRGINIKISGVQVNAKSIIQHEEEFEPLHKAVPSNPAERNKFQLTCRVKTPHFDVDWDIHDDTQLLLGIYEHGYGNWDLIKTDPDLKLSEKILLDDPEKKPQGKQLQMRTDYLLKMLKKDLESQDAIKAGDEPKIKKRKPRMKKKAPSNELVNEIASPRLSDNPSEEGEVKDDGAEKTPTKKKQKKDNKENKEKTGTPKKEKEGDKDKKRSKPKKEKAKPAGKGKKAQGPVHITQGSEPIPIGEEDDELDQETFSICKERMRPVKKALKQLDKPDEGLSVQDQLQHTRTCLLKIGDRITECLKSYSDPEHIKIWRRNLWIFVSKFTEFGAQKLHRLYKKAQKKRSQEGEKEHKKKDDPIKKKPFRPEPSGSSRDSTGAQPTTKPHPPVSHQSGPPHPPHHREQYNQHNKRHFPNDDRGDWQRDRKYGYPGNSNQPWQGDRHHPYEPHRYKDHHYSDRRPHGDYRSSGGYRNSGSPRKRPYDQYGNDRDHRGHRDYYDRHPDPKRRRSDEYRSPNYHQGGGGGGGGGGGGGHPQDFRRMPDHRGPPGNHGPMGPDHYRPFHPDKPPPLIDPRSPQSQKSPLEPISPGMDRSAEQKSATDFNWNNRKT

Target ORF information:

RefSeq Version	XM_021467691.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 2 (chd2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467691.1

ORF Insert Sequence:

ATGATGAAGA ATAAGAGTAA AAAGCAAGAA GACGAAGGCT CGACTCAAAG CAATGCTTCC 
AGCAACTCAG CATCCGAGGA ATCCAACCGC TCTGGGTCGG AGTCTGGAAG TCAGTCGGAG 
AGCGAGCAGG GCAGTGAAAA AGCACAATCC CGGCATTCTG AATCCAACAG CACGTCTGAT 
TCTGAGAGCC ACTCTGAGTC AGGCAGCGAA TCCAATGGGT CCAAATCTCA TCAGAGCTCA 
GCGCAGGTCA AAGACAAACC TGTCAGGAAG AAAGACAACC TGGCGGATGT GAAGAAGGTA 
CACAAAAAGA TGTGGGAAGA ACATCCTGAT GTTTATGGTG TGAGAAGATC CAGTCGCAGT 
AGACAAGAGC CTGCACGCCT TAATATTGGA GCTGAGGGCA GCAGTGATTC AGAGGGAGAA 
AGTTCCAAAC GAAAAAGCTC TCGGCAGAAA AAGAAAGAAA ATACCTGGAA AGATGATGAC 
TCAAATGACG AAGAGGATGT TGAGGAAGAG ACCAGCAGTT CAGAGAGTGA GCAGGAAGAG 
AAAAAAGTTA GATCCAGACG ACTTCCTGCT AGAAGACCAC AGACCAAATC ATCTGCTAGT 
AAACAGTCAC AAAAGGGACG AAAACAGAGA AAGCAGGAGT CCTCAGATGA AGATGATGAC 
GATGATGATG ATGACGAGGA GGACACTCCA AAAAGACAAA CACGGCGGCG GGCTGCTACT 
AAAGTCAGTT ACAAAGAGGA CCAGAATGAT TTTGAGACGG ACTCTGATGA CCTTATTGAA 
ATGGCAGAAG GGGCAGAGGA GCAGCAAGAT GATGACAGTG AGACCATTGA GAAGGTTGTT 
GACACTAGGA CTGGCAAGAA AGGAGCTACT GGAGCTTCCA CAACTCTCTA TGCCATTGAG 
GAAAACGGGG ACCCTGGAGC AGACTTTGAC CCGGAGAAAG AGGAAGGAGA AACTCAGTTC 
CTCATCAAGT GGAAAGGCTG GTCCTACATC CACAACACCT GGGAGAGTGT GGATTCCCTC 
ACTCAGCAGA AAGTCAAAGG CATGAAGAAG CTGGAAAACT TCAAGAAGAA AAATGAAGAG 
CTTAATGCCT GGTTGAAAAA AGCATCCCCT GAGGATTTAG AATATTACAA CTGTCAGCAA 
GAGCTCACAA ACGACCTGAG CAAGCAGTTC CAGATAATAG AGAGGGTGAT TGCAACAAGA 
ACAGGCAAAT CACAAGCAAC TTCAGACACT CCTTCTCATA AAAGCAGCTC TGGTGAGCCC 
GAGTACCTGT GTAAATGGAT GGGTCTGCCG TACGCTGAGT GCACATGGGA GGACAGCGCT 
TTAATCAAAA AGAAATTTCA GGCCTGCATT GACAGTTTCC ACAACAGAAA CAGCTCCAAG 
ACTGTCCCTT CCAAAGACTG TAAGGTGTTG AAGCAGAGGC CAAGATTTGT GCCCTTAAAG 
AAGCAGCCAT CGTACATTGG AGATGAGAAT CTTGAACTCA GAGATTACCA GCTAGATGGA 
GTGAACTGGC TGGCACACTC CTGGTGCAGG TGTAACAGTG TTATCTTGGC TGATGAAATG 
GGTTTAGGAA AGACCATTCA GACGATCTCC TTCCTCTCCT ACCTTTTTCA TCAGCACCAA 
CTTTACGGCC CCTTCATCGT GGTGGTGCCC CTGTCGACCC TCACGTCCTG GCAGAGGGAG 
TTTGACACTT GGGCCCCGGA CATGAATGTG GTGGTGTATC TGGGAGATGT CACAAGTAGA 
AAGACAATCC GAGACTATGA ATGGATCAAT CCACAGACAA AAAGGATCAA GTTTAATGCA 
CTTTTGACCA CATATGAAAT TCTACTGAAG GACAAGGGAG TGCTGGGGAA TATAAACTGG 
GCTTTCCTTG GAGTAGATGA AGCTCACCGG CTTAAGAATG ACGACTCGCT GCTGTATAAA 
ACTCTGATCG ACTTTCGATC CAATCACAGG CTGCTTATTA CCGGGACTCC TCTGCAAAAC 
TCCCTCAAAG AGCTGTGGTC CCTTCTGCAC TTTCTGATGT CTGACAAGTT TGAGTCCTGG 
GAGGATTTTG AGGATGAGCA TGGTAAAGGC AGGGATAATG GTTATCAGAG TTTGCACAAA 
GTTCTAGAGC CTTTCCTGCT ACGTCGTGTC AAGAAAGATG TGGAGAAATC CCTGCCGGCT 
AAAGTGGAGC AAATCCTCAG AGTCGACATG TCAGCCCAGC AAAAACAGTT CTACAAATGG 
ATCCTAACAA GAAACTTCAA GGCCTTGTCT AAAGGCACTA GAGGCAGCTC TTCTGGCTTT 
CTGAACATTG TCATGGAGCT GAAGAAGTGT TGTAACCACG CTTTCCTCAT CAAACAACCA 
GAAGATGGAG AAAATGATGC TCCACAAGAG CATCTACAGA GTTTGGTGCG TGGTGGAGGG 
AAACTGGTTC TCCTAGACAA GCTTCTCACA CGACTGAAGG ACAGAGGCAA CCGTGTGCTT 
ATCTTCTCTC AGATGGTCCG CATGTTGGAC ATTCTTGCTG ATTACTTGTC TATGAAACGA 
TATCAATTCC AGCGTTTGGA CGGATCCATC AAGGGTGAGC TCCGAAAACA AGCATTGGAC 
CACTTTAATG CAGAAGGCTC TGAGGACTTC TGTTTCCTGC TGTCCACCCG AGCCGGAGGG 
CTTGGGATTA ACTTGGCCTC TGCTGACACA GTGGTGATCT TTGACTCTGA CTGGAACCCA 
CAGAATGATC TTCAAGCTCA GGCCAGAGCC CACAGGATTG GCCAGAAAAA GCAAGTGAAT 
ATCTATCGTC TGGTCACAAA AGGGACTGTG GAGGAGGACA TTATTGAAAG AGCTAAAAAG 
AAGATGGTTC TAGACCATCT TGTCATTCAG AGAATGGACA CCACTGGTCG AACCGTACTG 
GATAACAGTT CAGCAAATTC AAATTCAAAT CCCTTCAATA AGGAAGAACT GTCAGCGATC 
TTGAAGTTTG GAGCAGAGGA TCTGTTTAAG GAAGCTGAGG GGGAGGAATC AGAACCGCAG 
GAAATGGACA TCGATGAGAT ATTGCGGCTT GCTGAAACCA GAGAGAGCGA TCAGGGATCC 
AGTGCTACCG ATGAGCTTCT GTCTCAGTTT AAGGTGGCTA ACTTCACCAT GGATGAAAGC 
ACACCAGATC TTGATGAAAA ACCTGGCCGG GACTGGGATG AAATAATTCC AGAAGAGCAG 
CGGCGGAAGG TGGAGGAAGA GCAGAAGCAG AAGGAGATGG AGGATATCTA CATGCTGCCA 
AGAAGCAGAA GCTCCAACAA GAAGGCACAA GCCAATGACA GCGACAGTGA TGTGGGATCC 
AAACTAAAGC ACAGATCCTC AGGTTCAGAC AGTGAAACCG ATGACAGCGA GGATGACAAG 
CGGCCTAAGA GGCGAGGAAG GCCAAGAGCC CGCAAAAACA ACGTGGAGGG ATTTACTGAT 
GCTGAGATCC GCAGGTTTAT AAAAGCATAC AAGAAATTTG GAGCTCCACT TGAGAGATTG 
GAGGCGATTG CCCGTGACTC TGAGCTGGTG GAGAAGTCCA TTGCAGACCT GAAGCGACTA 
GGGGAGCTGG TGCACAACAG CTGCGTGACT GCAGTACAGG AGCACGAAGA GCACTTGAAG 
GAAAACCCTG TGGAAGCTAA AGGCCCAGGG AAAAGACGAG GCATTAACAT AAAAATCTCT 
GGAGTTCAGG TCAATGCTAA ATCAATTATC CAGCATGAAG AGGAGTTTGA GCCCCTGCAT 
AAAGCTGTGC CTTCAAATCC TGCTGAAAGG AACAAATTTC AGTTGACCTG CCGGGTGAAA 
ACTCCTCACT TTGATGTGGA CTGGGATATT CACGATGACA CACAGTTGTT ATTGGGAATA 
TATGAGCACG GCTACGGCAA CTGGGACTTG ATCAAGACTG ATCCTGACCT CAAACTTTCA 
GAGAAGATTC TTCTTGATGA CCCTGAAAAG AAACCTCAAG GAAAGCAGTT ACAGATGCGA 
ACAGACTACT TGCTGAAGAT GCTGAAGAAA GACCTGGAAA GCCAAGATGC CATTAAAGCA 
GGAGATGAGC CCAAAATTAA AAAGAGAAAA CCTCGGATGA AGAAGAAGGC CCCTAGCAAT 
GAACTGGTCA ATGAAATCGC TTCTCCTCGT CTATCCGACA ACCCATCAGA AGAGGGAGAG 
GTAAAGGATG ATGGAGCTGA AAAAACACCC ACAAAGAAGA AACAGAAAAA GGACAACAAA 
GAGAACAAAG AAAAAACAGG GACTCCTAAA AAAGAGAAGG AGGGGGACAA AGACAAAAAG 
CGTTCCAAGC CCAAAAAAGA AAAGGCCAAA CCAGCAGGAA AAGGGAAGAA AGCCCAGGGC 
CCTGTGCACA TCACTCAAGG AAGTGAACCT ATACCAATTG GAGAGGAAGA TGATGAGCTG 
GACCAGGAAA CATTTAGCAT TTGTAAGGAG CGGATGCGGC CAGTGAAGAA AGCTCTTAAG 
CAGTTGGATA AGCCAGACGA GGGGCTGTCG GTGCAGGACC AGCTGCAGCA CACACGTACC 
TGTCTGCTGA AGATCGGAGA CCGCATTACA GAGTGCCTTA AGTCATATAG TGACCCCGAG 
CACATCAAGA TCTGGCGCAG AAACCTCTGG ATATTTGTGT CCAAATTTAC TGAGTTTGGT 
GCGCAGAAGC TGCATAGGCT ATATAAGAAG GCTCAGAAGA AGCGCTCCCA GGAGGGAGAG 
AAAGAGCATA AGAAAAAAGA CGATCCTATT AAGAAAAAGC CCTTTAGGCC TGAACCGTCT 
GGATCTAGCA GGGATTCAAC GGGTGCCCAG CCTACAACCA AACCCCATCC CCCTGTCAGC 
CATCAATCTG GACCTCCTCA TCCTCCACAC CATAGAGAAC AGTACAACCA GCATAATAAG 
AGGCATTTTC CTAATGATGA CAGGGGAGAT TGGCAAAGGG ACCGCAAATA TGGCTACCCA 
GGTAACAGCA ATCAGCCCTG GCAAGGAGAC CGTCATCACC CTTATGAGCC TCACAGGTAC 
AAGGATCATC ACTACAGTGA CCGTCGTCCT CATGGTGACT ACCGCAGCTC TGGAGGATAC 
CGTAACAGTG GCTCCCCTCG CAAACGGCCC TATGACCAGT ATGGCAACGA TAGAGATCAT 
CGGGGTCATC GAGACTATTA TGACAGACAT CCGGACCCAA AGAGAAGGCG CTCGGATGAA 
TATCGCTCTC CAAACTACCA CCAAGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA 
GGGCATCCGC AGGACTTTAG AAGAATGCCT GACCACAGGG GACCTCCTGG AAACCATGGC 
CCCATGGGGC CGGATCACTA CCGGCCCTTT CACCCAGACA AACCCCCGCC TCTGATTGAC 
CCACGTTCTC CGCAGTCTCA GAAATCGCCT CTTGAGCCGA TCTCACCTGG TATGGACAGA 
AGTGCAGAGC AGAAATCTGC GACAGACTTT AACTGGAACA ACAGGAAGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd2 ( XM_021467692.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323367.1 Danio rerio chromodomain helicase DNA binding protein 2 (chd2), transcript variant X4, mRNA.

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CloneID	ODa45854
Clone ID Related Accession (Same CDS sequence)	XM_021467692.1
Accession Version	XM_021467692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chromodomain-helicase-DNA-binding protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA ATAAGAGTAA AAAGCAAGAA GACGAAGGCT CGACTCAAAG CAATGCTTCC 
AGCAACTCAG CATCCGAGGA ATCCAACCGC TCTGGGTCGG AGTCTGGAAG TCAGTCGGAG 
AGCGAGCAGG GCAGTGAAAA AGCACAATCC CGGCATTCTG AATCCAACAG CACGTCTGAT 
TCTGAGAGCC ACTCTGAGTC AGGCAGCGAA TCCAATGGGT CCAAATCTCA TCAGAGCTCA 
GCGCAGGTCA AAGACAAACC TGTCAGGAAG AAAGACAACC TGGCGGATGT GAAGAAGGTA 
CACAAAAAGA TGTGGGAAGA ACATCCTGAT GTTTATGGTG TGAGAAGATC CAGTCGCAGT 
AGACAAGAGC CTGCACGCCT TAATATTGGA GCTGAGGGCA GCAGTGATTC AGAGGGAGAA 
AGTTCCAAAC GAAAAAGCTC TCGGCAGAAA AAGAAAGAAC CACAGACCAA ATCATCTGCT 
AGTAAACAGT CACAAAAGGG ACGAAAACAG AGAAAGCAGG AGTCCTCAGA TGAAGATGAT 
GACGATGATG ATGATGACGA GGAGGACACT CCAAAAAGAC AAACACGGCG GCGGGCTGCT 
ACTAAAGTCA GTTACAAAGA GGACCAGAAT GATTTTGAGA CGGACTCTGA TGACCTTATT 
GAAATGGCAG AAGGGGCAGA GGAGCAGCAA GATGATGACA GTGAGACCAT TGAGAAGGTT 
GTTGACACTA GGACTGGCAA GAAAGGAGCT ACTGGAGCTT CCACAACTCT CTATGCCATT 
GAGGAAAACG GGGACCCTGG AGCAGACTTT GACCCGGAGA AAGAGGAAGG AGAAACTCAG 
TTCCTCATCA AGTGGAAAGG CTGGTCCTAC ATCCACAACA CCTGGGAGAG TGTGGATTCC 
CTCACTCAGC AGAAAGTCAA AGGCATGAAG AAGCTGGAAA ACTTCAAGAA GAAAAATGAA 
GAGCTTAATG CCTGGTTGAA AAAAGCATCC CCTGAGGATT TAGAATATTA CAACTGTCAG 
CAAGAGCTCA CAAACGACCT GAGCAAGCAG TTCCAGATAA TAGAGAGGGT GATTGCAACA 
AGAACAGGCA AATCACAAGC AACTTCAGAC ACTCCTTCTC ATAAAAGCAG CTCTGGTGAG 
CCCGAGTACC TGTGTAAATG GATGGGTCTG CCGTACGCTG AGTGCACATG GGAGGACAGC 
GCTTTAATCA AAAAGAAATT TCAGGCCTGC ATTGACAGTT TCCACAACAG AAACAGCTCC 
AAGACTGTCC CTTCCAAAGA CTGTAAGGTG TTGAAGCAGA GGCCAAGATT TGTGCCCTTA 
AAGAAGCAGC CATCGTACAT TGGAGATGAG AATCTTGAAC TCAGAGATTA CCAGCTAGAT 
GGAGTGAACT GGCTGGCACA CTCCTGGTGC AGGTGTAACA GTGTTATCTT GGCTGATGAA 
ATGGGTTTAG GAAAGACCAT TCAGACGATC TCCTTCCTCT CCTACCTTTT TCATCAGCAC 
CAACTTTACG GCCCCTTCAT CGTGGTGGTG CCCCTGTCGA CCCTCACGTC CTGGCAGAGG 
GAGTTTGACA CTTGGGCCCC GGACATGAAT GTGGTGGTGT ATCTGGGAGA TGTCACAAGT 
AGAAAGACAA TCCGAGACTA TGAATGGATC AATCCACAGA CAAAAAGGAT CAAGTTTAAT 
GCACTTTTGA CCACATATGA AATTCTACTG AAGGACAAGG GAGTGCTGGG GAATATAAAC 
TGGGCTTTCC TTGGAGTAGA TGAAGCTCAC CGGCTTAAGA ATGACGACTC GCTGCTGTAT 
AAAACTCTGA TCGACTTTCG ATCCAATCAC AGGCTGCTTA TTACCGGGAC TCCTCTGCAA 
AACTCCCTCA AAGAGCTGTG GTCCCTTCTG CACTTTCTGA TGTCTGACAA GTTTGAGTCC 
TGGGAGGATT TTGAGGATGA GCATGGTAAA GGCAGGGATA ATGGTTATCA GAGTTTGCAC 
AAAGTTCTAG AGCCTTTCCT GCTACGTCGT GTCAAGAAAG ATGTGGAGAA ATCCCTGCCG 
GCTAAAGTGG AGCAAATCCT CAGAGTCGAC ATGTCAGCCC AGCAAAAACA GTTCTACAAA 
TGGATCCTAA CAAGAAACTT CAAGGCCTTG TCTAAAGGCA CTAGAGGCAG CTCTTCTGGC 
TTTCTGAACA TTGTCATGGA GCTGAAGAAG TGTTGTAACC ACGCTTTCCT CATCAAACAA 
CCAGAAGATG GAGAAAATGA TGCTCCACAA GAGCATCTAC AGAGTTTGGT GCGTGGTGGA 
GGGAAACTGG TTCTCCTAGA CAAGCTTCTC ACACGACTGA AGGACAGAGG CAACCGTGTG 
CTTATCTTCT CTCAGATGGT CCGCATGTTG GACATTCTTG CTGATTACTT GTCTATGAAA 
CGATATCAAT TCCAGCGTTT GGACGGATCC ATCAAGGGTG AGCTCCGAAA ACAAGCATTG 
GACCACTTTA ATGCAGAAGG CTCTGAGGAC TTCTGTTTCC TGCTGTCCAC CCGAGCCGGA 
GGGCTTGGGA TTAACTTGGC CTCTGCTGAC ACAGTGGTGA TCTTTGACTC TGACTGGAAC 
CCACAGAATG ATCTTCAAGC TCAGGCCAGA GCCCACAGGA TTGGCCAGAA AAAGCAAGTG 
AATATCTATC GTCTGGTCAC AAAAGGGACT GTGGAGGAGG ACATTATTGA AAGAGCTAAA 
AAGAAGATGG TTCTAGACCA TCTTGTCATT CAGAGAATGG ACACCACTGG TCGAACCGTA 
CTGGATAACA GTTCAGCAAA TTCAAATTCA AATCCCTTCA ATAAGGAAGA ACTGTCAGCG 
ATCTTGAAGT TTGGAGCAGA GGATCTGTTT AAGGAAGCTG AGGGGGAGGA ATCAGAACCG 
CAGGAAATGG ACATCGATGA GATATTGCGG CTTGCTGAAA CCAGAGAGAG CGATCAGGGA 
TCCAGTGCTA CCGATGAGCT TCTGTCTCAG TTTAAGGTGG CTAACTTCAC CATGGATGAA 
AGCACACCAG ATCTTGATGA AAAACCTGGC CGGGACTGGG ATGAAATAAT TCCAGAAGAG 
CAGCGGCGGA AGGTGGAGGA AGAGCAGAAG CAGAAGGAGA TGGAGGATAT CTACATGCTG 
CCAAGAAGCA GAAGCTCCAA CAAGAAGGCA CAAGCCAATG ACAGCGACAG TGATGTGGGA 
TCCAAACTAA AGCACAGATC CTCAGGTTCA GACAGTGAAA CCGATGACAG CGAGGATGAC 
AAGCGGCCTA AGAGGCGAGG AAGGCCAAGA GCCCGCAAAA ACAACGTGGA GGGATTTACT 
GATGCTGAGA TCCGCAGGTT TATAAAAGCA TACAAGAAAT TTGGAGCTCC ACTTGAGAGA 
TTGGAGGCGA TTGCCCGTGA CTCTGAGCTG GTGGAGAAGT CCATTGCAGA CCTGAAGCGA 
CTAGGGGAGC TGGTGCACAA CAGCTGCGTG ACTGCAGTAC AGGAGCACGA AGAGCACTTG 
AAGGAAAACC CTGTGGAAGC TAAAGGCCCA GGGAAAAGAC GAGGCATTAA CATAAAAATC 
TCTGGAGTTC AGGTCAATGC TAAATCAATT ATCCAGCATG AAGAGGAGTT TGAGCCCCTG 
CATAAAGCTG TGCCTTCAAA TCCTGCTGAA AGGAACAAAT TTCAGTTGAC CTGCCGGGTG 
AAAACTCCTC ACTTTGATGT GGACTGGGAT ATTCACGATG ACACACAGTT GTTATTGGGA 
ATATATGAGC ACGGCTACGG CAACTGGGAC TTGATCAAGA CTGATCCTGA CCTCAAACTT 
TCAGAGAAGA TTCTTCTTGA TGACCCTGAA AAGAAACCTC AAGGAAAGCA GTTACAGATG 
CGAACAGACT ACTTGCTGAA GATGCTGAAG AAAGACCTGG AAAGCCAAGA TGCCATTAAA 
GCAGGAGATG AGCCCAAAAT TAAAAAGAGA AAACCTCGGA TGAAGAAGAA GGCCCCTAGC 
AATGAACTGG TCAATGAAAT CGCTTCTCCT CGTCTATCCG ACAACCCATC AGAAGAGGGA 
GAGGTAAAGG ATGATGGAGC TGAAAAAACA CCCACAAAGA AGAAACAGAA AAAGGACAAC 
AAAGAGAACA AAGAAAAAAC AGGGACTCCT AAAAAAGAGA AGGAGGGGGA CAAAGACAAA 
AAGCGTTCCA AGCCCAAAAA AGAAAAGGCC AAACCAGCAG GAAAAGGGAA GAAAGCCCAG 
GGCCCTGTGC ACATCACTCA AGGAAGTGAA CCTATACCAA TTGGAGAGGA AGATGATGAG 
CTGGACCAGG AAACATTTAG CATTTGTAAG GAGCGGATGC GGCCAGTGAA GAAAGCTCTT 
AAGCAGTTGG ATAAGCCAGA CGAGGGGCTG TCGGTGCAGG ACCAGCTGCA GCACACACGT 
ACCTGTCTGC TGAAGATCGG AGACCGCATT ACAGAGTGCC TTAAGTCATA TAGTGACCCC 
GAGCACATCA AGATCTGGCG CAGAAACCTC TGGATATTTG TGTCCAAATT TACTGAGTTT 
GGTGCGCAGA AGCTGCATAG GCTATATAAG AAGGCTCAGA AGAAGCGCTC CCAGGAGGGA 
GAGAAAGAGC ATAAGAAAAA AGACGATCCT ATTAAGAAAA AGCCCTTTAG GCCTGAACCG 
TCTGGATCTA GCAGGGATTC AACGGGTGCC CAGCCTACAA CCAAACCCCA TCCCCCTGTC 
AGCCATCAAT CTGGACCTCC TCATCCTCCA CACCATAGAG AACAGTACAA CCAGCATAAT 
AAGAGGCATT TTCCTAATGA TGACAGGGGA GATTGGCAAA GGGACCGCAA ATATGGCTAC 
CCAGGTAACA GCAATCAGCC CTGGCAAGGA GACCGTCATC ACCCTTATGA GCCTCACAGG 
TACAAGGATC ATCACTACAG TGACCGTCGT CCTCATGGTG ACTACCGCAG CTCTGGAGGA 
TACCGTAACA GTGGCTCCCC TCGCAAACGG CCCTATGACC AGTATGGCAA CGATAGAGAT 
CATCGGGGTC ATCGAGACTA TTATGACAGA CATCCGGACC CAAAGAGAAG GCGCTCGGAT 
GAATATCGCT CTCCAAACTA CCACCAAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA 
GGAGGGCATC CGCAGGACTT TAGAAGAATG CCTGACCACA GGGGACCTCC TGGAAACCAT 
GGCCCCATGG GGCCGGATCA CTACCGGCCC TTTCACCCAG ACAAACCCCC GCCTCTGATT 
GACCCACGTT CTCCGCAGTC TCAGAAATCG CCTCTTGAGC CGATCTCACC TGGTATGGAC 
AGAAGTGCAG AGCAGAAATC TGCGACAGAC TTTAACTGGA ACAACAGGAA GACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323367.1
CDS	1941..7277
Translation	MMKNKSKKQEDEGSTQSNASSNSASEESNRSGSESGSQSESEQGSEKAQSRHSESNSTSDSESHSESGSESNGSKSHQSSAQVKDKPVRKKDNLADVKKVHKKMWEEHPDVYGVRRSSRSRQEPARLNIGAEGSSDSEGESSKRKSSRQKKKEPQTKSSASKQSQKGRKQRKQESSDEDDDDDDDDEEDTPKRQTRRRAATKVSYKEDQNDFETDSDDLIEMAEGAEEQQDDDSETIEKVVDTRTGKKGATGASTTLYAIEENGDPGADFDPEKEEGETQFLIKWKGWSYIHNTWESVDSLTQQKVKGMKKLENFKKKNEELNAWLKKASPEDLEYYNCQQELTNDLSKQFQIIERVIATRTGKSQATSDTPSHKSSSGEPEYLCKWMGLPYAECTWEDSALIKKKFQACIDSFHNRNSSKTVPSKDCKVLKQRPRFVPLKKQPSYIGDENLELRDYQLDGVNWLAHSWCRCNSVILADEMGLGKTIQTISFLSYLFHQHQLYGPFIVVVPLSTLTSWQREFDTWAPDMNVVVYLGDVTSRKTIRDYEWINPQTKRIKFNALLTTYEILLKDKGVLGNINWAFLGVDEAHRLKNDDSLLYKTLIDFRSNHRLLITGTPLQNSLKELWSLLHFLMSDKFESWEDFEDEHGKGRDNGYQSLHKVLEPFLLRRVKKDVEKSLPAKVEQILRVDMSAQQKQFYKWILTRNFKALSKGTRGSSSGFLNIVMELKKCCNHAFLIKQPEDGENDAPQEHLQSLVRGGGKLVLLDKLLTRLKDRGNRVLIFSQMVRMLDILADYLSMKRYQFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGTVEEDIIERAKKKMVLDHLVIQRMDTTGRTVLDNSSANSNSNPFNKEELSAILKFGAEDLFKEAEGEESEPQEMDIDEILRLAETRESDQGSSATDELLSQFKVANFTMDESTPDLDEKPGRDWDEIIPEEQRRKVEEEQKQKEMEDIYMLPRSRSSNKKAQANDSDSDVGSKLKHRSSGSDSETDDSEDDKRPKRRGRPRARKNNVEGFTDAEIRRFIKAYKKFGAPLERLEAIARDSELVEKSIADLKRLGELVHNSCVTAVQEHEEHLKENPVEAKGPGKRRGINIKISGVQVNAKSIIQHEEEFEPLHKAVPSNPAERNKFQLTCRVKTPHFDVDWDIHDDTQLLLGIYEHGYGNWDLIKTDPDLKLSEKILLDDPEKKPQGKQLQMRTDYLLKMLKKDLESQDAIKAGDEPKIKKRKPRMKKKAPSNELVNEIASPRLSDNPSEEGEVKDDGAEKTPTKKKQKKDNKENKEKTGTPKKEKEGDKDKKRSKPKKEKAKPAGKGKKAQGPVHITQGSEPIPIGEEDDELDQETFSICKERMRPVKKALKQLDKPDEGLSVQDQLQHTRTCLLKIGDRITECLKSYSDPEHIKIWRRNLWIFVSKFTEFGAQKLHRLYKKAQKKRSQEGEKEHKKKDDPIKKKPFRPEPSGSSRDSTGAQPTTKPHPPVSHQSGPPHPPHHREQYNQHNKRHFPNDDRGDWQRDRKYGYPGNSNQPWQGDRHHPYEPHRYKDHHYSDRRPHGDYRSSGGYRNSGSPRKRPYDQYGNDRDHRGHRDYYDRHPDPKRRRSDEYRSPNYHQGGGGGGGGGGGGGHPQDFRRMPDHRGPPGNHGPMGPDHYRPFHPDKPPPLIDPRSPQSQKSPLEPISPGMDRSAEQKSATDFNWNNRKT

Target ORF information:

RefSeq Version	XM_021467692.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 2 (chd2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467692.1

ORF Insert Sequence:

ATGATGAAGA ATAAGAGTAA AAAGCAAGAA GACGAAGGCT CGACTCAAAG CAATGCTTCC 
AGCAACTCAG CATCCGAGGA ATCCAACCGC TCTGGGTCGG AGTCTGGAAG TCAGTCGGAG 
AGCGAGCAGG GCAGTGAAAA AGCACAATCC CGGCATTCTG AATCCAACAG CACGTCTGAT 
TCTGAGAGCC ACTCTGAGTC AGGCAGCGAA TCCAATGGGT CCAAATCTCA TCAGAGCTCA 
GCGCAGGTCA AAGACAAACC TGTCAGGAAG AAAGACAACC TGGCGGATGT GAAGAAGGTA 
CACAAAAAGA TGTGGGAAGA ACATCCTGAT GTTTATGGTG TGAGAAGATC CAGTCGCAGT 
AGACAAGAGC CTGCACGCCT TAATATTGGA GCTGAGGGCA GCAGTGATTC AGAGGGAGAA 
AGTTCCAAAC GAAAAAGCTC TCGGCAGAAA AAGAAAGAAC CACAGACCAA ATCATCTGCT 
AGTAAACAGT CACAAAAGGG ACGAAAACAG AGAAAGCAGG AGTCCTCAGA TGAAGATGAT 
GACGATGATG ATGATGACGA GGAGGACACT CCAAAAAGAC AAACACGGCG GCGGGCTGCT 
ACTAAAGTCA GTTACAAAGA GGACCAGAAT GATTTTGAGA CGGACTCTGA TGACCTTATT 
GAAATGGCAG AAGGGGCAGA GGAGCAGCAA GATGATGACA GTGAGACCAT TGAGAAGGTT 
GTTGACACTA GGACTGGCAA GAAAGGAGCT ACTGGAGCTT CCACAACTCT CTATGCCATT 
GAGGAAAACG GGGACCCTGG AGCAGACTTT GACCCGGAGA AAGAGGAAGG AGAAACTCAG 
TTCCTCATCA AGTGGAAAGG CTGGTCCTAC ATCCACAACA CCTGGGAGAG TGTGGATTCC 
CTCACTCAGC AGAAAGTCAA AGGCATGAAG AAGCTGGAAA ACTTCAAGAA GAAAAATGAA 
GAGCTTAATG CCTGGTTGAA AAAAGCATCC CCTGAGGATT TAGAATATTA CAACTGTCAG 
CAAGAGCTCA CAAACGACCT GAGCAAGCAG TTCCAGATAA TAGAGAGGGT GATTGCAACA 
AGAACAGGCA AATCACAAGC AACTTCAGAC ACTCCTTCTC ATAAAAGCAG CTCTGGTGAG 
CCCGAGTACC TGTGTAAATG GATGGGTCTG CCGTACGCTG AGTGCACATG GGAGGACAGC 
GCTTTAATCA AAAAGAAATT TCAGGCCTGC ATTGACAGTT TCCACAACAG AAACAGCTCC 
AAGACTGTCC CTTCCAAAGA CTGTAAGGTG TTGAAGCAGA GGCCAAGATT TGTGCCCTTA 
AAGAAGCAGC CATCGTACAT TGGAGATGAG AATCTTGAAC TCAGAGATTA CCAGCTAGAT 
GGAGTGAACT GGCTGGCACA CTCCTGGTGC AGGTGTAACA GTGTTATCTT GGCTGATGAA 
ATGGGTTTAG GAAAGACCAT TCAGACGATC TCCTTCCTCT CCTACCTTTT TCATCAGCAC 
CAACTTTACG GCCCCTTCAT CGTGGTGGTG CCCCTGTCGA CCCTCACGTC CTGGCAGAGG 
GAGTTTGACA CTTGGGCCCC GGACATGAAT GTGGTGGTGT ATCTGGGAGA TGTCACAAGT 
AGAAAGACAA TCCGAGACTA TGAATGGATC AATCCACAGA CAAAAAGGAT CAAGTTTAAT 
GCACTTTTGA CCACATATGA AATTCTACTG AAGGACAAGG GAGTGCTGGG GAATATAAAC 
TGGGCTTTCC TTGGAGTAGA TGAAGCTCAC CGGCTTAAGA ATGACGACTC GCTGCTGTAT 
AAAACTCTGA TCGACTTTCG ATCCAATCAC AGGCTGCTTA TTACCGGGAC TCCTCTGCAA 
AACTCCCTCA AAGAGCTGTG GTCCCTTCTG CACTTTCTGA TGTCTGACAA GTTTGAGTCC 
TGGGAGGATT TTGAGGATGA GCATGGTAAA GGCAGGGATA ATGGTTATCA GAGTTTGCAC 
AAAGTTCTAG AGCCTTTCCT GCTACGTCGT GTCAAGAAAG ATGTGGAGAA ATCCCTGCCG 
GCTAAAGTGG AGCAAATCCT CAGAGTCGAC ATGTCAGCCC AGCAAAAACA GTTCTACAAA 
TGGATCCTAA CAAGAAACTT CAAGGCCTTG TCTAAAGGCA CTAGAGGCAG CTCTTCTGGC 
TTTCTGAACA TTGTCATGGA GCTGAAGAAG TGTTGTAACC ACGCTTTCCT CATCAAACAA 
CCAGAAGATG GAGAAAATGA TGCTCCACAA GAGCATCTAC AGAGTTTGGT GCGTGGTGGA 
GGGAAACTGG TTCTCCTAGA CAAGCTTCTC ACACGACTGA AGGACAGAGG CAACCGTGTG 
CTTATCTTCT CTCAGATGGT CCGCATGTTG GACATTCTTG CTGATTACTT GTCTATGAAA 
CGATATCAAT TCCAGCGTTT GGACGGATCC ATCAAGGGTG AGCTCCGAAA ACAAGCATTG 
GACCACTTTA ATGCAGAAGG CTCTGAGGAC TTCTGTTTCC TGCTGTCCAC CCGAGCCGGA 
GGGCTTGGGA TTAACTTGGC CTCTGCTGAC ACAGTGGTGA TCTTTGACTC TGACTGGAAC 
CCACAGAATG ATCTTCAAGC TCAGGCCAGA GCCCACAGGA TTGGCCAGAA AAAGCAAGTG 
AATATCTATC GTCTGGTCAC AAAAGGGACT GTGGAGGAGG ACATTATTGA AAGAGCTAAA 
AAGAAGATGG TTCTAGACCA TCTTGTCATT CAGAGAATGG ACACCACTGG TCGAACCGTA 
CTGGATAACA GTTCAGCAAA TTCAAATTCA AATCCCTTCA ATAAGGAAGA ACTGTCAGCG 
ATCTTGAAGT TTGGAGCAGA GGATCTGTTT AAGGAAGCTG AGGGGGAGGA ATCAGAACCG 
CAGGAAATGG ACATCGATGA GATATTGCGG CTTGCTGAAA CCAGAGAGAG CGATCAGGGA 
TCCAGTGCTA CCGATGAGCT TCTGTCTCAG TTTAAGGTGG CTAACTTCAC CATGGATGAA 
AGCACACCAG ATCTTGATGA AAAACCTGGC CGGGACTGGG ATGAAATAAT TCCAGAAGAG 
CAGCGGCGGA AGGTGGAGGA AGAGCAGAAG CAGAAGGAGA TGGAGGATAT CTACATGCTG 
CCAAGAAGCA GAAGCTCCAA CAAGAAGGCA CAAGCCAATG ACAGCGACAG TGATGTGGGA 
TCCAAACTAA AGCACAGATC CTCAGGTTCA GACAGTGAAA CCGATGACAG CGAGGATGAC 
AAGCGGCCTA AGAGGCGAGG AAGGCCAAGA GCCCGCAAAA ACAACGTGGA GGGATTTACT 
GATGCTGAGA TCCGCAGGTT TATAAAAGCA TACAAGAAAT TTGGAGCTCC ACTTGAGAGA 
TTGGAGGCGA TTGCCCGTGA CTCTGAGCTG GTGGAGAAGT CCATTGCAGA CCTGAAGCGA 
CTAGGGGAGC TGGTGCACAA CAGCTGCGTG ACTGCAGTAC AGGAGCACGA AGAGCACTTG 
AAGGAAAACC CTGTGGAAGC TAAAGGCCCA GGGAAAAGAC GAGGCATTAA CATAAAAATC 
TCTGGAGTTC AGGTCAATGC TAAATCAATT ATCCAGCATG AAGAGGAGTT TGAGCCCCTG 
CATAAAGCTG TGCCTTCAAA TCCTGCTGAA AGGAACAAAT TTCAGTTGAC CTGCCGGGTG 
AAAACTCCTC ACTTTGATGT GGACTGGGAT ATTCACGATG ACACACAGTT GTTATTGGGA 
ATATATGAGC ACGGCTACGG CAACTGGGAC TTGATCAAGA CTGATCCTGA CCTCAAACTT 
TCAGAGAAGA TTCTTCTTGA TGACCCTGAA AAGAAACCTC AAGGAAAGCA GTTACAGATG 
CGAACAGACT ACTTGCTGAA GATGCTGAAG AAAGACCTGG AAAGCCAAGA TGCCATTAAA 
GCAGGAGATG AGCCCAAAAT TAAAAAGAGA AAACCTCGGA TGAAGAAGAA GGCCCCTAGC 
AATGAACTGG TCAATGAAAT CGCTTCTCCT CGTCTATCCG ACAACCCATC AGAAGAGGGA 
GAGGTAAAGG ATGATGGAGC TGAAAAAACA CCCACAAAGA AGAAACAGAA AAAGGACAAC 
AAAGAGAACA AAGAAAAAAC AGGGACTCCT AAAAAAGAGA AGGAGGGGGA CAAAGACAAA 
AAGCGTTCCA AGCCCAAAAA AGAAAAGGCC AAACCAGCAG GAAAAGGGAA GAAAGCCCAG 
GGCCCTGTGC ACATCACTCA AGGAAGTGAA CCTATACCAA TTGGAGAGGA AGATGATGAG 
CTGGACCAGG AAACATTTAG CATTTGTAAG GAGCGGATGC GGCCAGTGAA GAAAGCTCTT 
AAGCAGTTGG ATAAGCCAGA CGAGGGGCTG TCGGTGCAGG ACCAGCTGCA GCACACACGT 
ACCTGTCTGC TGAAGATCGG AGACCGCATT ACAGAGTGCC TTAAGTCATA TAGTGACCCC 
GAGCACATCA AGATCTGGCG CAGAAACCTC TGGATATTTG TGTCCAAATT TACTGAGTTT 
GGTGCGCAGA AGCTGCATAG GCTATATAAG AAGGCTCAGA AGAAGCGCTC CCAGGAGGGA 
GAGAAAGAGC ATAAGAAAAA AGACGATCCT ATTAAGAAAA AGCCCTTTAG GCCTGAACCG 
TCTGGATCTA GCAGGGATTC AACGGGTGCC CAGCCTACAA CCAAACCCCA TCCCCCTGTC 
AGCCATCAAT CTGGACCTCC TCATCCTCCA CACCATAGAG AACAGTACAA CCAGCATAAT 
AAGAGGCATT TTCCTAATGA TGACAGGGGA GATTGGCAAA GGGACCGCAA ATATGGCTAC 
CCAGGTAACA GCAATCAGCC CTGGCAAGGA GACCGTCATC ACCCTTATGA GCCTCACAGG 
TACAAGGATC ATCACTACAG TGACCGTCGT CCTCATGGTG ACTACCGCAG CTCTGGAGGA 
TACCGTAACA GTGGCTCCCC TCGCAAACGG CCCTATGACC AGTATGGCAA CGATAGAGAT 
CATCGGGGTC ATCGAGACTA TTATGACAGA CATCCGGACC CAAAGAGAAG GCGCTCGGAT 
GAATATCGCT CTCCAAACTA CCACCAAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA 
GGAGGGCATC CGCAGGACTT TAGAAGAATG CCTGACCACA GGGGACCTCC TGGAAACCAT 
GGCCCCATGG GGCCGGATCA CTACCGGCCC TTTCACCCAG ACAAACCCCC GCCTCTGATT 
GACCCACGTT CTCCGCAGTC TCAGAAATCG CCTCTTGAGC CGATCTCACC TGGTATGGAC 
AGAAGTGCAG AGCAGAAATC TGCGACAGAC TTTAACTGGA ACAACAGGAA GACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd2 ( XM_009293623.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291898.1 Danio rerio chromodomain helicase DNA binding protein 2 (chd2), transcript variant X1, mRNA.

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CloneID	ODa45853
Clone ID Related Accession (Same CDS sequence)	XM_009293623.2 , XM_009293623.3
Accession Version	XM_009293623.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	chromodomain-helicase-DNA-binding protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293623.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA ATAAGAGTAA AAAGCAAGAA GACGAAGGCT CGACTCAAAG CAATGCTTCC 
AGCAACTCAG CATCCGAGGA ATCCAACCGC TCTGGGTCGG AGTCTGGAAG TCAGTCGGAG 
AGCGAGCAGG GCAGTGAAAA AGCACAATCC CGGCATTCTG AATCCAACAG CACGTCTGAT 
TCTGAGAGCC ACTCTGAGTC AGGCAGCGAA TCCAATGGGT CCAAATCTCA TCAGAGCTCA 
GCGCAGGTCA AAGACAAACC TGTCAGGAAG AAAGACAACC TGGCGGATGT GAAGAAGATG 
TGGGAAGAAC ATCCTGATGT TTATGGTGTG AGAAGATCCA GTCGCAGTAG ACAAGAGCCT 
GCACGCCTTA ATATTGGAGC TGAGGGCAGC AGTGATTCAG AGGGAGAAAG TTCCAAACGA 
AAAAGCTCTC GGCAGAAAAA GAAAGAAAAT ACCTGGAAAG ATGATGACTC AAATGACGAA 
GAGGATGTTG AGGAAGAGAC CAGCAGTTCA GAGAGTGAGC AGGAAGAGAA AAAAGTTAGA 
TCCAGACGAC TTCCTGCTAG AAGACCACAG ACCAAATCAT CTGCTAGTAA ACAGTCACAA 
AAGGGACGAA AACAGAGAAA GCAGGAGTCC TCAGATGAAG ATGATGACGA TGATGATGAT 
GACGAGGAGG ACACTCCAAA AAGACAAACA CGGCGGCGGG CTGCTACTAA AGTCAGTTAC 
AAAGAGGACC AGAATGATTT TGAGACGGAC TCTGATGACC TTATTGAAAT GGCAGAAGGG 
GCAGAGGAGC AGCAAGATGA TGACAGTGAG ACCATTGAGA AGGTTGTTGA CACTAGGACT 
GGCAAGAAAG GAGCTACTGG AGCTTCCACA ACTCTCTATG CCATTGAGGA AAACGGGGAC 
CCTGGAGCAG ACTTTGACCC GGAGAAAGAG GAAGGAGAAA CTCAGTTCCT CATCAAGTGG 
AAAGGCTGGT CCTACATCCA CAACACCTGG GAGAGTGTGG ATTCCCTCAC TCAGCAGAAA 
GTCAAAGGCA TGAAGAAGCT GGAAAACTTC AAGAAGAAAA ATGAAGAGCT TAATGCCTGG 
TTGAAAAAAG CATCCCCTGA GGATTTAGAA TATTACAACT GTCAGCAAGA GCTCACAAAC 
GACCTGAGCA AGCAGTTCCA GATAATAGAG AGGGTGATTG CAACAAGAAC AGGCAAATCA 
CAAGCAACTT CAGACACTCC TTCTCATAAA AGCAGCTCTG GTGAGCCCGA GTACCTGTGT 
AAATGGATGG GTCTGCCGTA CGCTGAGTGC ACATGGGAGG ACAGCGCTTT AATCAAAAAG 
AAATTTCAGG CCTGCATTGA CAGTTTCCAC AACAGAAACA GCTCCAAGAC TGTCCCTTCC 
AAAGACTGTA AGGTGTTGAA GCAGAGGCCA AGATTTGTGC CCTTAAAGAA GCAGCCATCG 
TACATTGGAG ATGAGAATCT TGAACTCAGA GATTACCAGC TAGATGGAGT GAACTGGCTG 
GCACACTCCT GGTGCAGGTG TAACAGTGTT ATCTTGGCTG ATGAAATGGG TTTAGGAAAG 
ACCATTCAGA CGATCTCCTT CCTCTCCTAC CTTTTTCATC AGCACCAACT TTACGGCCCC 
TTCATCGTGG TGGTGCCCCT GTCGACCCTC ACGTCCTGGC AGAGGGAGTT TGACACTTGG 
GCCCCGGACA TGAATGTGGT GGTGTATCTG GGAGATGTCA CAAGTAGAAA GACAATCCGA 
GACTATGAAT GGATCAATCC ACAGACAAAA AGGATCAAGT TTAATGCACT TTTGACCACA 
TATGAAATTC TACTGAAGGA CAAGGGAGTG CTGGGGAATA TAAACTGGGC TTTCCTTGGA 
GTAGATGAAG CTCACCGGCT TAAGAATGAC GACTCGCTGC TGTATAAAAC TCTGATCGAC 
TTTCGATCCA ATCACAGGCT GCTTATTACC GGGACTCCTC TGCAAAACTC CCTCAAAGAG 
CTGTGGTCCC TTCTGCACTT TCTGATGTCT GACAAGTTTG AGTCCTGGGA GGATTTTGAG 
GATGAGCATG GTAAAGGCAG GGATAATGGT TATCAGAGTT TGCACAAAGT TCTAGAGCCT 
TTCCTGCTAC GTCGTGTCAA GAAAGATGTG GAGAAATCCC TGCCGGCTAA AGTGGAGCAA 
ATCCTCAGAG TCGACATGTC AGCCCAGCAA AAACAGTTCT ACAAATGGAT CCTAACAAGA 
AACTTCAAGG CCTTGTCTAA AGGCACTAGA GGCAGCTCTT CTGGCTTTCT GAACATTGTC 
ATGGAGCTGA AGAAGTGTTG TAACCACGCT TTCCTCATCA AACAACCAGA AGATGGAGAA 
AATGATGCTC CACAAGAGCA TCTACAGAGT TTGGTGCGTG GTGGAGGGAA ACTGGTTCTC 
CTAGACAAGC TTCTCACACG ACTGAAGGAC AGAGGCAACC GTGTGCTTAT CTTCTCTCAG 
ATGGTCCGCA TGTTGGACAT TCTTGCTGAT TACTTGTCTA TGAAACGATA TCAATTCCAG 
CGTTTGGACG GATCCATCAA GGGTGAGCTC CGAAAACAAG CATTGGACCA CTTTAATGCA 
GAAGGCTCTG AGGACTTCTG TTTCCTGCTG TCCACCCGAG CCGGAGGGCT TGGGATTAAC 
TTGGCCTCTG CTGACACAGT GGTGATCTTT GACTCTGACT GGAACCCACA GAATGATCTT 
CAAGCTCAGG CCAGAGCCCA CAGGATTGGC CAGAAAAAGC AAGTGAATAT CTATCGTCTG 
GTCACAAAAG GGACTGTGGA GGAGGACATT ATTGAAAGAG CTAAAAAGAA GATGGTTCTA 
GACCATCTTG TCATTCAGAG AATGGACACC ACTGGTCGAA CCGTACTGGA TAACAGTTCA 
GCAAATTCAA ATTCAAATCC CTTCAATAAG GAAGAACTGT CAGCGATCTT GAAGTTTGGA 
GCAGAGGATC TGTTTAAGGA AGCTGAGGGG GAGGAATCAG AACCGCAGGA AATGGACATC 
GATGAGATAT TGCGGCTTGC TGAAACCAGA GAGAGCGATC AGGGATCCAG TGCTACCGAT 
GAGCTTCTGT CTCAGTTTAA GGTGGCTAAC TTCACCATGG ATGAAAGCAC ACCAGATCTT 
GATGAAAAAC CTGGCCGGGA CTGGGATGAA ATAATTCCAG AAGAGCAGCG GCGGAAGGTG 
GAGGAAGAGC AGAAGCAGAA GGAGATGGAG GATATCTACA TGCTGCCAAG AAGCAGAAGC 
TCCAACAAGA AGGCACAAGC CAATGACAGC GACAGTGATG TGGGATCCAA ACTAAAGCAC 
AGATCCTCAG GTTCAGACAG TGAAACCGAT GACAGCGAGG ATGACAAGCG GCCTAAGAGG 
CGAGGAAGGC CAAGAGCCCG CAAAAACAAC GTGGAGGGAT TTACTGATGC TGAGATCCGC 
AGGTTTATAA AAGCATACAA GAAATTTGGA GCTCCACTTG AGAGATTGGA GGCGATTGCC 
CGTGACTCTG AGCTGGTGGA GAAGTCCATT GCAGACCTGA AGCGACTAGG GGAGCTGGTG 
CACAACAGCT GCGTGACTGC AGTACAGGAG CACGAAGAGC ACTTGAAGGA AAACCCTGTG 
GAAGCTAAAG GCCCAGGGAA AAGACGAGGC ATTAACATAA AAATCTCTGG AGTTCAGGTC 
AATGCTAAAT CAATTATCCA GCATGAAGAG GAGTTTGAGC CCCTGCATAA AGCTGTGCCT 
TCAAATCCTG CTGAAAGGAA CAAATTTCAG TTGACCTGCC GGGTGAAAAC TCCTCACTTT 
GATGTGGACT GGGATATTCA CGATGACACA CAGTTGTTAT TGGGAATATA TGAGCACGGC 
TACGGCAACT GGGACTTGAT CAAGACTGAT CCTGACCTCA AACTTTCAGA GAAGATTCTT 
CTTGATGACC CTGAAAAGAA ACCTCAAGGA AAGCAGTTAC AGATGCGAAC AGACTACTTG 
CTGAAGATGC TGAAGAAAGA CCTGGAAAGC CAAGATGCCA TTAAAGCAGG AGATGAGCCC 
AAAATTAAAA AGAGAAAACC TCGGATGAAG AAGAAGGCCC CTAGCAATGA ACTGGTCAAT 
GAAATCGCTT CTCCTCGTCT ATCCGACAAC CCATCAGAAG AGGGAGAGGT AAAGGATGAT 
GGAGCTGAAA AAACACCCAC AAAGAAGAAA CAGAAAAAGG ACAACAAAGA GAACAAAGAA 
AAAACAGGGA CTCCTAAAAA AGAGAAGGAG GGGGACAAAG ACAAAAAGCG TTCCAAGCCC 
AAAAAAGAAA AGGCCAAACC AGCAGGAAAA GGGAAGAAAG CCCAGGGCCC TGTGCACATC 
ACTCAAGGAA GTGAACCTAT ACCAATTGGA GAGGAAGATG ATGAGCTGGA CCAGGAAACA 
TTTAGCATTT GTAAGGAGCG GATGCGGCCA GTGAAGAAAG CTCTTAAGCA GTTGGATAAG 
CCAGACGAGG GGCTGTCGGT GCAGGACCAG CTGCAGCACA CACGTACCTG TCTGCTGAAG 
ATCGGAGACC GCATTACAGA GTGCCTTAAG TCATATAGTG ACCCCGAGCA CATCAAGATC 
TGGCGCAGAA ACCTCTGGAT ATTTGTGTCC AAATTTACTG AGTTTGGTGC GCAGAAGCTG 
CATAGGCTAT ATAAGAAGGC TCAGAAGAAG CGCTCCCAGG AGGGAGAGAA AGAGCATAAG 
AAAAAAGACG ATCCTATTAA GAAAAAGCCC TTTAGGCCTG AACCGTCTGG ATCTAGCAGG 
GATTCAACGG GTGCCCAGCC TACAACCAAA CCCCATCCCC CTGTCAGCCA TCAATCTGGA 
CCTCCTCATC CTCCACACCA TAGAGAACAG TACAACCAGC ATAATAAGAG GCATTTTCCT 
AATGATGACA GGGGAGATTG GCAAAGGGAC CGCAAATATG GCTACCCAGG TAACAGCAAT 
CAGCCCTGGC AAGGAGACCG TCATCACCCT TATGAGCCTC ACAGGTACAA GGATCATCAC 
TACAGTGACC GTCGTCCTCA TGGTGACTAC CGCAGCTCTG GAGGATACCG TAACAGTGGC 
TCCCCTCGCA AACGGCCCTA TGACCAGTAT GGCAACGATA GAGATCATCG GGGTCATCGA 
GACTATTATG ACAGACATCC GGACCCAAAG AGAAGGCGCT CGGATGAATA TCGCTCTCCA 
AACTACCACC AAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGAGGAGG GCATCCGCAG 
GACTTTAGAA GAATGCCTGA CCACAGGGGA CCTCCTGGAA ACCATGGCCC CATGGGGCCG 
GATCACTACC GGCCCTTTCA CCCAGACAAA CCCCCGCCTC TGATTGACCC ACGTTCTCCG 
CAGTCTCAGA AATCGCCTCT TGAGCCGATC TCACCTGGTA TGGACAGAAG TGCAGAGCAG 
AAATCTGCGA CAGACTTTAA CTGGAACAAC AGGAAGACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291898.1
CDS	1941..7382
Translation	MMKNKSKKQEDEGSTQSNASSNSASEESNRSGSESGSQSESEQGSEKAQSRHSESNSTSDSESHSESGSESNGSKSHQSSAQVKDKPVRKKDNLADVKKMWEEHPDVYGVRRSSRSRQEPARLNIGAEGSSDSEGESSKRKSSRQKKKENTWKDDDSNDEEDVEEETSSSESEQEEKKVRSRRLPARRPQTKSSASKQSQKGRKQRKQESSDEDDDDDDDDEEDTPKRQTRRRAATKVSYKEDQNDFETDSDDLIEMAEGAEEQQDDDSETIEKVVDTRTGKKGATGASTTLYAIEENGDPGADFDPEKEEGETQFLIKWKGWSYIHNTWESVDSLTQQKVKGMKKLENFKKKNEELNAWLKKASPEDLEYYNCQQELTNDLSKQFQIIERVIATRTGKSQATSDTPSHKSSSGEPEYLCKWMGLPYAECTWEDSALIKKKFQACIDSFHNRNSSKTVPSKDCKVLKQRPRFVPLKKQPSYIGDENLELRDYQLDGVNWLAHSWCRCNSVILADEMGLGKTIQTISFLSYLFHQHQLYGPFIVVVPLSTLTSWQREFDTWAPDMNVVVYLGDVTSRKTIRDYEWINPQTKRIKFNALLTTYEILLKDKGVLGNINWAFLGVDEAHRLKNDDSLLYKTLIDFRSNHRLLITGTPLQNSLKELWSLLHFLMSDKFESWEDFEDEHGKGRDNGYQSLHKVLEPFLLRRVKKDVEKSLPAKVEQILRVDMSAQQKQFYKWILTRNFKALSKGTRGSSSGFLNIVMELKKCCNHAFLIKQPEDGENDAPQEHLQSLVRGGGKLVLLDKLLTRLKDRGNRVLIFSQMVRMLDILADYLSMKRYQFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGTVEEDIIERAKKKMVLDHLVIQRMDTTGRTVLDNSSANSNSNPFNKEELSAILKFGAEDLFKEAEGEESEPQEMDIDEILRLAETRESDQGSSATDELLSQFKVANFTMDESTPDLDEKPGRDWDEIIPEEQRRKVEEEQKQKEMEDIYMLPRSRSSNKKAQANDSDSDVGSKLKHRSSGSDSETDDSEDDKRPKRRGRPRARKNNVEGFTDAEIRRFIKAYKKFGAPLERLEAIARDSELVEKSIADLKRLGELVHNSCVTAVQEHEEHLKENPVEAKGPGKRRGINIKISGVQVNAKSIIQHEEEFEPLHKAVPSNPAERNKFQLTCRVKTPHFDVDWDIHDDTQLLLGIYEHGYGNWDLIKTDPDLKLSEKILLDDPEKKPQGKQLQMRTDYLLKMLKKDLESQDAIKAGDEPKIKKRKPRMKKKAPSNELVNEIASPRLSDNPSEEGEVKDDGAEKTPTKKKQKKDNKENKEKTGTPKKEKEGDKDKKRSKPKKEKAKPAGKGKKAQGPVHITQGSEPIPIGEEDDELDQETFSICKERMRPVKKALKQLDKPDEGLSVQDQLQHTRTCLLKIGDRITECLKSYSDPEHIKIWRRNLWIFVSKFTEFGAQKLHRLYKKAQKKRSQEGEKEHKKKDDPIKKKPFRPEPSGSSRDSTGAQPTTKPHPPVSHQSGPPHPPHHREQYNQHNKRHFPNDDRGDWQRDRKYGYPGNSNQPWQGDRHHPYEPHRYKDHHYSDRRPHGDYRSSGGYRNSGSPRKRPYDQYGNDRDHRGHRDYYDRHPDPKRRRSDEYRSPNYHQGGGGGGGGGGGGGHPQDFRRMPDHRGPPGNHGPMGPDHYRPFHPDKPPPLIDPRSPQSQKSPLEPISPGMDRSAEQKSATDFNWNNRKT

Target ORF information:

RefSeq Version	XM_009293623.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 2 (chd2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293623.2

ORF Insert Sequence:

ATGATGAAGA ATAAGAGTAA AAAGCAAGAA GACGAAGGCT CGACTCAAAG CAATGCTTCC 
AGCAACTCAG CATCCGAGGA ATCCAACCGC TCTGGGTCGG AGTCTGGAAG TCAGTCGGAG 
AGCGAGCAGG GCAGTGAAAA AGCACAATCC CGGCATTCTG AATCCAACAG CACGTCTGAT 
TCTGAGAGCC ACTCTGAGTC AGGCAGCGAA TCCAATGGGT CCAAATCTCA TCAGAGCTCA 
GCGCAGGTCA AAGACAAACC TGTCAGGAAG AAAGACAACC TGGCGGATGT GAAGAAGATG 
TGGGAAGAAC ATCCTGATGT TTATGGTGTG AGAAGATCCA GTCGCAGTAG ACAAGAGCCT 
GCACGCCTTA ATATTGGAGC TGAGGGCAGC AGTGATTCAG AGGGAGAAAG TTCCAAACGA 
AAAAGCTCTC GGCAGAAAAA GAAAGAAAAT ACCTGGAAAG ATGATGACTC AAATGACGAA 
GAGGATGTTG AGGAAGAGAC CAGCAGTTCA GAGAGTGAGC AGGAAGAGAA AAAAGTTAGA 
TCCAGACGAC TTCCTGCTAG AAGACCACAG ACCAAATCAT CTGCTAGTAA ACAGTCACAA 
AAGGGACGAA AACAGAGAAA GCAGGAGTCC TCAGATGAAG ATGATGACGA TGATGATGAT 
GACGAGGAGG ACACTCCAAA AAGACAAACA CGGCGGCGGG CTGCTACTAA AGTCAGTTAC 
AAAGAGGACC AGAATGATTT TGAGACGGAC TCTGATGACC TTATTGAAAT GGCAGAAGGG 
GCAGAGGAGC AGCAAGATGA TGACAGTGAG ACCATTGAGA AGGTTGTTGA CACTAGGACT 
GGCAAGAAAG GAGCTACTGG AGCTTCCACA ACTCTCTATG CCATTGAGGA AAACGGGGAC 
CCTGGAGCAG ACTTTGACCC GGAGAAAGAG GAAGGAGAAA CTCAGTTCCT CATCAAGTGG 
AAAGGCTGGT CCTACATCCA CAACACCTGG GAGAGTGTGG ATTCCCTCAC TCAGCAGAAA 
GTCAAAGGCA TGAAGAAGCT GGAAAACTTC AAGAAGAAAA ATGAAGAGCT TAATGCCTGG 
TTGAAAAAAG CATCCCCTGA GGATTTAGAA TATTACAACT GTCAGCAAGA GCTCACAAAC 
GACCTGAGCA AGCAGTTCCA GATAATAGAG AGGGTGATTG CAACAAGAAC AGGCAAATCA 
CAAGCAACTT CAGACACTCC TTCTCATAAA AGCAGCTCTG GTGAGCCCGA GTACCTGTGT 
AAATGGATGG GTCTGCCGTA CGCTGAGTGC ACATGGGAGG ACAGCGCTTT AATCAAAAAG 
AAATTTCAGG CCTGCATTGA CAGTTTCCAC AACAGAAACA GCTCCAAGAC TGTCCCTTCC 
AAAGACTGTA AGGTGTTGAA GCAGAGGCCA AGATTTGTGC CCTTAAAGAA GCAGCCATCG 
TACATTGGAG ATGAGAATCT TGAACTCAGA GATTACCAGC TAGATGGAGT GAACTGGCTG 
GCACACTCCT GGTGCAGGTG TAACAGTGTT ATCTTGGCTG ATGAAATGGG TTTAGGAAAG 
ACCATTCAGA CGATCTCCTT CCTCTCCTAC CTTTTTCATC AGCACCAACT TTACGGCCCC 
TTCATCGTGG TGGTGCCCCT GTCGACCCTC ACGTCCTGGC AGAGGGAGTT TGACACTTGG 
GCCCCGGACA TGAATGTGGT GGTGTATCTG GGAGATGTCA CAAGTAGAAA GACAATCCGA 
GACTATGAAT GGATCAATCC ACAGACAAAA AGGATCAAGT TTAATGCACT TTTGACCACA 
TATGAAATTC TACTGAAGGA CAAGGGAGTG CTGGGGAATA TAAACTGGGC TTTCCTTGGA 
GTAGATGAAG CTCACCGGCT TAAGAATGAC GACTCGCTGC TGTATAAAAC TCTGATCGAC 
TTTCGATCCA ATCACAGGCT GCTTATTACC GGGACTCCTC TGCAAAACTC CCTCAAAGAG 
CTGTGGTCCC TTCTGCACTT TCTGATGTCT GACAAGTTTG AGTCCTGGGA GGATTTTGAG 
GATGAGCATG GTAAAGGCAG GGATAATGGT TATCAGAGTT TGCACAAAGT TCTAGAGCCT 
TTCCTGCTAC GTCGTGTCAA GAAAGATGTG GAGAAATCCC TGCCGGCTAA AGTGGAGCAA 
ATCCTCAGAG TCGACATGTC AGCCCAGCAA AAACAGTTCT ACAAATGGAT CCTAACAAGA 
AACTTCAAGG CCTTGTCTAA AGGCACTAGA GGCAGCTCTT CTGGCTTTCT GAACATTGTC 
ATGGAGCTGA AGAAGTGTTG TAACCACGCT TTCCTCATCA AACAACCAGA AGATGGAGAA 
AATGATGCTC CACAAGAGCA TCTACAGAGT TTGGTGCGTG GTGGAGGGAA ACTGGTTCTC 
CTAGACAAGC TTCTCACACG ACTGAAGGAC AGAGGCAACC GTGTGCTTAT CTTCTCTCAG 
ATGGTCCGCA TGTTGGACAT TCTTGCTGAT TACTTGTCTA TGAAACGATA TCAATTCCAG 
CGTTTGGACG GATCCATCAA GGGTGAGCTC CGAAAACAAG CATTGGACCA CTTTAATGCA 
GAAGGCTCTG AGGACTTCTG TTTCCTGCTG TCCACCCGAG CCGGAGGGCT TGGGATTAAC 
TTGGCCTCTG CTGACACAGT GGTGATCTTT GACTCTGACT GGAACCCACA GAATGATCTT 
CAAGCTCAGG CCAGAGCCCA CAGGATTGGC CAGAAAAAGC AAGTGAATAT CTATCGTCTG 
GTCACAAAAG GGACTGTGGA GGAGGACATT ATTGAAAGAG CTAAAAAGAA GATGGTTCTA 
GACCATCTTG TCATTCAGAG AATGGACACC ACTGGTCGAA CCGTACTGGA TAACAGTTCA 
GCAAATTCAA ATTCAAATCC CTTCAATAAG GAAGAACTGT CAGCGATCTT GAAGTTTGGA 
GCAGAGGATC TGTTTAAGGA AGCTGAGGGG GAGGAATCAG AACCGCAGGA AATGGACATC 
GATGAGATAT TGCGGCTTGC TGAAACCAGA GAGAGCGATC AGGGATCCAG TGCTACCGAT 
GAGCTTCTGT CTCAGTTTAA GGTGGCTAAC TTCACCATGG ATGAAAGCAC ACCAGATCTT 
GATGAAAAAC CTGGCCGGGA CTGGGATGAA ATAATTCCAG AAGAGCAGCG GCGGAAGGTG 
GAGGAAGAGC AGAAGCAGAA GGAGATGGAG GATATCTACA TGCTGCCAAG AAGCAGAAGC 
TCCAACAAGA AGGCACAAGC CAATGACAGC GACAGTGATG TGGGATCCAA ACTAAAGCAC 
AGATCCTCAG GTTCAGACAG TGAAACCGAT GACAGCGAGG ATGACAAGCG GCCTAAGAGG 
CGAGGAAGGC CAAGAGCCCG CAAAAACAAC GTGGAGGGAT TTACTGATGC TGAGATCCGC 
AGGTTTATAA AAGCATACAA GAAATTTGGA GCTCCACTTG AGAGATTGGA GGCGATTGCC 
CGTGACTCTG AGCTGGTGGA GAAGTCCATT GCAGACCTGA AGCGACTAGG GGAGCTGGTG 
CACAACAGCT GCGTGACTGC AGTACAGGAG CACGAAGAGC ACTTGAAGGA AAACCCTGTG 
GAAGCTAAAG GCCCAGGGAA AAGACGAGGC ATTAACATAA AAATCTCTGG AGTTCAGGTC 
AATGCTAAAT CAATTATCCA GCATGAAGAG GAGTTTGAGC CCCTGCATAA AGCTGTGCCT 
TCAAATCCTG CTGAAAGGAA CAAATTTCAG TTGACCTGCC GGGTGAAAAC TCCTCACTTT 
GATGTGGACT GGGATATTCA CGATGACACA CAGTTGTTAT TGGGAATATA TGAGCACGGC 
TACGGCAACT GGGACTTGAT CAAGACTGAT CCTGACCTCA AACTTTCAGA GAAGATTCTT 
CTTGATGACC CTGAAAAGAA ACCTCAAGGA AAGCAGTTAC AGATGCGAAC AGACTACTTG 
CTGAAGATGC TGAAGAAAGA CCTGGAAAGC CAAGATGCCA TTAAAGCAGG AGATGAGCCC 
AAAATTAAAA AGAGAAAACC TCGGATGAAG AAGAAGGCCC CTAGCAATGA ACTGGTCAAT 
GAAATCGCTT CTCCTCGTCT ATCCGACAAC CCATCAGAAG AGGGAGAGGT AAAGGATGAT 
GGAGCTGAAA AAACACCCAC AAAGAAGAAA CAGAAAAAGG ACAACAAAGA GAACAAAGAA 
AAAACAGGGA CTCCTAAAAA AGAGAAGGAG GGGGACAAAG ACAAAAAGCG TTCCAAGCCC 
AAAAAAGAAA AGGCCAAACC AGCAGGAAAA GGGAAGAAAG CCCAGGGCCC TGTGCACATC 
ACTCAAGGAA GTGAACCTAT ACCAATTGGA GAGGAAGATG ATGAGCTGGA CCAGGAAACA 
TTTAGCATTT GTAAGGAGCG GATGCGGCCA GTGAAGAAAG CTCTTAAGCA GTTGGATAAG 
CCAGACGAGG GGCTGTCGGT GCAGGACCAG CTGCAGCACA CACGTACCTG TCTGCTGAAG 
ATCGGAGACC GCATTACAGA GTGCCTTAAG TCATATAGTG ACCCCGAGCA CATCAAGATC 
TGGCGCAGAA ACCTCTGGAT ATTTGTGTCC AAATTTACTG AGTTTGGTGC GCAGAAGCTG 
CATAGGCTAT ATAAGAAGGC TCAGAAGAAG CGCTCCCAGG AGGGAGAGAA AGAGCATAAG 
AAAAAAGACG ATCCTATTAA GAAAAAGCCC TTTAGGCCTG AACCGTCTGG ATCTAGCAGG 
GATTCAACGG GTGCCCAGCC TACAACCAAA CCCCATCCCC CTGTCAGCCA TCAATCTGGA 
CCTCCTCATC CTCCACACCA TAGAGAACAG TACAACCAGC ATAATAAGAG GCATTTTCCT 
AATGATGACA GGGGAGATTG GCAAAGGGAC CGCAAATATG GCTACCCAGG TAACAGCAAT 
CAGCCCTGGC AAGGAGACCG TCATCACCCT TATGAGCCTC ACAGGTACAA GGATCATCAC 
TACAGTGACC GTCGTCCTCA TGGTGACTAC CGCAGCTCTG GAGGATACCG TAACAGTGGC 
TCCCCTCGCA AACGGCCCTA TGACCAGTAT GGCAACGATA GAGATCATCG GGGTCATCGA 
GACTATTATG ACAGACATCC GGACCCAAAG AGAAGGCGCT CGGATGAATA TCGCTCTCCA 
AACTACCACC AAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGAGGAGG GCATCCGCAG 
GACTTTAGAA GAATGCCTGA CCACAGGGGA CCTCCTGGAA ACCATGGCCC CATGGGGCCG 
GATCACTACC GGCCCTTTCA CCCAGACAAA CCCCCGCCTC TGATTGACCC ACGTTCTCCG 
CAGTCTCAGA AATCGCCTCT TGAGCCGATC TCACCTGGTA TGGACAGAAG TGCAGAGCAG 
AAATCTGCGA CAGACTTTAA CTGGAACAAC AGGAAGACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd2 ( XM_009293623.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291898.1 Danio rerio chromodomain helicase DNA binding protein 2 (chd2), transcript variant X3, mRNA.

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CloneID	ODa45853
Clone ID Related Accession (Same CDS sequence)	XM_009293623.2 , XM_009293623.3
Accession Version	XM_009293623.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chromodomain-helicase-DNA-binding protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293623.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA ATAAGAGTAA AAAGCAAGAA GACGAAGGCT CGACTCAAAG CAATGCTTCC 
AGCAACTCAG CATCCGAGGA ATCCAACCGC TCTGGGTCGG AGTCTGGAAG TCAGTCGGAG 
AGCGAGCAGG GCAGTGAAAA AGCACAATCC CGGCATTCTG AATCCAACAG CACGTCTGAT 
TCTGAGAGCC ACTCTGAGTC AGGCAGCGAA TCCAATGGGT CCAAATCTCA TCAGAGCTCA 
GCGCAGGTCA AAGACAAACC TGTCAGGAAG AAAGACAACC TGGCGGATGT GAAGAAGATG 
TGGGAAGAAC ATCCTGATGT TTATGGTGTG AGAAGATCCA GTCGCAGTAG ACAAGAGCCT 
GCACGCCTTA ATATTGGAGC TGAGGGCAGC AGTGATTCAG AGGGAGAAAG TTCCAAACGA 
AAAAGCTCTC GGCAGAAAAA GAAAGAAAAT ACCTGGAAAG ATGATGACTC AAATGACGAA 
GAGGATGTTG AGGAAGAGAC CAGCAGTTCA GAGAGTGAGC AGGAAGAGAA AAAAGTTAGA 
TCCAGACGAC TTCCTGCTAG AAGACCACAG ACCAAATCAT CTGCTAGTAA ACAGTCACAA 
AAGGGACGAA AACAGAGAAA GCAGGAGTCC TCAGATGAAG ATGATGACGA TGATGATGAT 
GACGAGGAGG ACACTCCAAA AAGACAAACA CGGCGGCGGG CTGCTACTAA AGTCAGTTAC 
AAAGAGGACC AGAATGATTT TGAGACGGAC TCTGATGACC TTATTGAAAT GGCAGAAGGG 
GCAGAGGAGC AGCAAGATGA TGACAGTGAG ACCATTGAGA AGGTTGTTGA CACTAGGACT 
GGCAAGAAAG GAGCTACTGG AGCTTCCACA ACTCTCTATG CCATTGAGGA AAACGGGGAC 
CCTGGAGCAG ACTTTGACCC GGAGAAAGAG GAAGGAGAAA CTCAGTTCCT CATCAAGTGG 
AAAGGCTGGT CCTACATCCA CAACACCTGG GAGAGTGTGG ATTCCCTCAC TCAGCAGAAA 
GTCAAAGGCA TGAAGAAGCT GGAAAACTTC AAGAAGAAAA ATGAAGAGCT TAATGCCTGG 
TTGAAAAAAG CATCCCCTGA GGATTTAGAA TATTACAACT GTCAGCAAGA GCTCACAAAC 
GACCTGAGCA AGCAGTTCCA GATAATAGAG AGGGTGATTG CAACAAGAAC AGGCAAATCA 
CAAGCAACTT CAGACACTCC TTCTCATAAA AGCAGCTCTG GTGAGCCCGA GTACCTGTGT 
AAATGGATGG GTCTGCCGTA CGCTGAGTGC ACATGGGAGG ACAGCGCTTT AATCAAAAAG 
AAATTTCAGG CCTGCATTGA CAGTTTCCAC AACAGAAACA GCTCCAAGAC TGTCCCTTCC 
AAAGACTGTA AGGTGTTGAA GCAGAGGCCA AGATTTGTGC CCTTAAAGAA GCAGCCATCG 
TACATTGGAG ATGAGAATCT TGAACTCAGA GATTACCAGC TAGATGGAGT GAACTGGCTG 
GCACACTCCT GGTGCAGGTG TAACAGTGTT ATCTTGGCTG ATGAAATGGG TTTAGGAAAG 
ACCATTCAGA CGATCTCCTT CCTCTCCTAC CTTTTTCATC AGCACCAACT TTACGGCCCC 
TTCATCGTGG TGGTGCCCCT GTCGACCCTC ACGTCCTGGC AGAGGGAGTT TGACACTTGG 
GCCCCGGACA TGAATGTGGT GGTGTATCTG GGAGATGTCA CAAGTAGAAA GACAATCCGA 
GACTATGAAT GGATCAATCC ACAGACAAAA AGGATCAAGT TTAATGCACT TTTGACCACA 
TATGAAATTC TACTGAAGGA CAAGGGAGTG CTGGGGAATA TAAACTGGGC TTTCCTTGGA 
GTAGATGAAG CTCACCGGCT TAAGAATGAC GACTCGCTGC TGTATAAAAC TCTGATCGAC 
TTTCGATCCA ATCACAGGCT GCTTATTACC GGGACTCCTC TGCAAAACTC CCTCAAAGAG 
CTGTGGTCCC TTCTGCACTT TCTGATGTCT GACAAGTTTG AGTCCTGGGA GGATTTTGAG 
GATGAGCATG GTAAAGGCAG GGATAATGGT TATCAGAGTT TGCACAAAGT TCTAGAGCCT 
TTCCTGCTAC GTCGTGTCAA GAAAGATGTG GAGAAATCCC TGCCGGCTAA AGTGGAGCAA 
ATCCTCAGAG TCGACATGTC AGCCCAGCAA AAACAGTTCT ACAAATGGAT CCTAACAAGA 
AACTTCAAGG CCTTGTCTAA AGGCACTAGA GGCAGCTCTT CTGGCTTTCT GAACATTGTC 
ATGGAGCTGA AGAAGTGTTG TAACCACGCT TTCCTCATCA AACAACCAGA AGATGGAGAA 
AATGATGCTC CACAAGAGCA TCTACAGAGT TTGGTGCGTG GTGGAGGGAA ACTGGTTCTC 
CTAGACAAGC TTCTCACACG ACTGAAGGAC AGAGGCAACC GTGTGCTTAT CTTCTCTCAG 
ATGGTCCGCA TGTTGGACAT TCTTGCTGAT TACTTGTCTA TGAAACGATA TCAATTCCAG 
CGTTTGGACG GATCCATCAA GGGTGAGCTC CGAAAACAAG CATTGGACCA CTTTAATGCA 
GAAGGCTCTG AGGACTTCTG TTTCCTGCTG TCCACCCGAG CCGGAGGGCT TGGGATTAAC 
TTGGCCTCTG CTGACACAGT GGTGATCTTT GACTCTGACT GGAACCCACA GAATGATCTT 
CAAGCTCAGG CCAGAGCCCA CAGGATTGGC CAGAAAAAGC AAGTGAATAT CTATCGTCTG 
GTCACAAAAG GGACTGTGGA GGAGGACATT ATTGAAAGAG CTAAAAAGAA GATGGTTCTA 
GACCATCTTG TCATTCAGAG AATGGACACC ACTGGTCGAA CCGTACTGGA TAACAGTTCA 
GCAAATTCAA ATTCAAATCC CTTCAATAAG GAAGAACTGT CAGCGATCTT GAAGTTTGGA 
GCAGAGGATC TGTTTAAGGA AGCTGAGGGG GAGGAATCAG AACCGCAGGA AATGGACATC 
GATGAGATAT TGCGGCTTGC TGAAACCAGA GAGAGCGATC AGGGATCCAG TGCTACCGAT 
GAGCTTCTGT CTCAGTTTAA GGTGGCTAAC TTCACCATGG ATGAAAGCAC ACCAGATCTT 
GATGAAAAAC CTGGCCGGGA CTGGGATGAA ATAATTCCAG AAGAGCAGCG GCGGAAGGTG 
GAGGAAGAGC AGAAGCAGAA GGAGATGGAG GATATCTACA TGCTGCCAAG AAGCAGAAGC 
TCCAACAAGA AGGCACAAGC CAATGACAGC GACAGTGATG TGGGATCCAA ACTAAAGCAC 
AGATCCTCAG GTTCAGACAG TGAAACCGAT GACAGCGAGG ATGACAAGCG GCCTAAGAGG 
CGAGGAAGGC CAAGAGCCCG CAAAAACAAC GTGGAGGGAT TTACTGATGC TGAGATCCGC 
AGGTTTATAA AAGCATACAA GAAATTTGGA GCTCCACTTG AGAGATTGGA GGCGATTGCC 
CGTGACTCTG AGCTGGTGGA GAAGTCCATT GCAGACCTGA AGCGACTAGG GGAGCTGGTG 
CACAACAGCT GCGTGACTGC AGTACAGGAG CACGAAGAGC ACTTGAAGGA AAACCCTGTG 
GAAGCTAAAG GCCCAGGGAA AAGACGAGGC ATTAACATAA AAATCTCTGG AGTTCAGGTC 
AATGCTAAAT CAATTATCCA GCATGAAGAG GAGTTTGAGC CCCTGCATAA AGCTGTGCCT 
TCAAATCCTG CTGAAAGGAA CAAATTTCAG TTGACCTGCC GGGTGAAAAC TCCTCACTTT 
GATGTGGACT GGGATATTCA CGATGACACA CAGTTGTTAT TGGGAATATA TGAGCACGGC 
TACGGCAACT GGGACTTGAT CAAGACTGAT CCTGACCTCA AACTTTCAGA GAAGATTCTT 
CTTGATGACC CTGAAAAGAA ACCTCAAGGA AAGCAGTTAC AGATGCGAAC AGACTACTTG 
CTGAAGATGC TGAAGAAAGA CCTGGAAAGC CAAGATGCCA TTAAAGCAGG AGATGAGCCC 
AAAATTAAAA AGAGAAAACC TCGGATGAAG AAGAAGGCCC CTAGCAATGA ACTGGTCAAT 
GAAATCGCTT CTCCTCGTCT ATCCGACAAC CCATCAGAAG AGGGAGAGGT AAAGGATGAT 
GGAGCTGAAA AAACACCCAC AAAGAAGAAA CAGAAAAAGG ACAACAAAGA GAACAAAGAA 
AAAACAGGGA CTCCTAAAAA AGAGAAGGAG GGGGACAAAG ACAAAAAGCG TTCCAAGCCC 
AAAAAAGAAA AGGCCAAACC AGCAGGAAAA GGGAAGAAAG CCCAGGGCCC TGTGCACATC 
ACTCAAGGAA GTGAACCTAT ACCAATTGGA GAGGAAGATG ATGAGCTGGA CCAGGAAACA 
TTTAGCATTT GTAAGGAGCG GATGCGGCCA GTGAAGAAAG CTCTTAAGCA GTTGGATAAG 
CCAGACGAGG GGCTGTCGGT GCAGGACCAG CTGCAGCACA CACGTACCTG TCTGCTGAAG 
ATCGGAGACC GCATTACAGA GTGCCTTAAG TCATATAGTG ACCCCGAGCA CATCAAGATC 
TGGCGCAGAA ACCTCTGGAT ATTTGTGTCC AAATTTACTG AGTTTGGTGC GCAGAAGCTG 
CATAGGCTAT ATAAGAAGGC TCAGAAGAAG CGCTCCCAGG AGGGAGAGAA AGAGCATAAG 
AAAAAAGACG ATCCTATTAA GAAAAAGCCC TTTAGGCCTG AACCGTCTGG ATCTAGCAGG 
GATTCAACGG GTGCCCAGCC TACAACCAAA CCCCATCCCC CTGTCAGCCA TCAATCTGGA 
CCTCCTCATC CTCCACACCA TAGAGAACAG TACAACCAGC ATAATAAGAG GCATTTTCCT 
AATGATGACA GGGGAGATTG GCAAAGGGAC CGCAAATATG GCTACCCAGG TAACAGCAAT 
CAGCCCTGGC AAGGAGACCG TCATCACCCT TATGAGCCTC ACAGGTACAA GGATCATCAC 
TACAGTGACC GTCGTCCTCA TGGTGACTAC CGCAGCTCTG GAGGATACCG TAACAGTGGC 
TCCCCTCGCA AACGGCCCTA TGACCAGTAT GGCAACGATA GAGATCATCG GGGTCATCGA 
GACTATTATG ACAGACATCC GGACCCAAAG AGAAGGCGCT CGGATGAATA TCGCTCTCCA 
AACTACCACC AAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGAGGAGG GCATCCGCAG 
GACTTTAGAA GAATGCCTGA CCACAGGGGA CCTCCTGGAA ACCATGGCCC CATGGGGCCG 
GATCACTACC GGCCCTTTCA CCCAGACAAA CCCCCGCCTC TGATTGACCC ACGTTCTCCG 
CAGTCTCAGA AATCGCCTCT TGAGCCGATC TCACCTGGTA TGGACAGAAG TGCAGAGCAG 
AAATCTGCGA CAGACTTTAA CTGGAACAAC AGGAAGACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291898.1
CDS	1941..7382
Translation	MMKNKSKKQEDEGSTQSNASSNSASEESNRSGSESGSQSESEQGSEKAQSRHSESNSTSDSESHSESGSESNGSKSHQSSAQVKDKPVRKKDNLADVKKMWEEHPDVYGVRRSSRSRQEPARLNIGAEGSSDSEGESSKRKSSRQKKKENTWKDDDSNDEEDVEEETSSSESEQEEKKVRSRRLPARRPQTKSSASKQSQKGRKQRKQESSDEDDDDDDDDEEDTPKRQTRRRAATKVSYKEDQNDFETDSDDLIEMAEGAEEQQDDDSETIEKVVDTRTGKKGATGASTTLYAIEENGDPGADFDPEKEEGETQFLIKWKGWSYIHNTWESVDSLTQQKVKGMKKLENFKKKNEELNAWLKKASPEDLEYYNCQQELTNDLSKQFQIIERVIATRTGKSQATSDTPSHKSSSGEPEYLCKWMGLPYAECTWEDSALIKKKFQACIDSFHNRNSSKTVPSKDCKVLKQRPRFVPLKKQPSYIGDENLELRDYQLDGVNWLAHSWCRCNSVILADEMGLGKTIQTISFLSYLFHQHQLYGPFIVVVPLSTLTSWQREFDTWAPDMNVVVYLGDVTSRKTIRDYEWINPQTKRIKFNALLTTYEILLKDKGVLGNINWAFLGVDEAHRLKNDDSLLYKTLIDFRSNHRLLITGTPLQNSLKELWSLLHFLMSDKFESWEDFEDEHGKGRDNGYQSLHKVLEPFLLRRVKKDVEKSLPAKVEQILRVDMSAQQKQFYKWILTRNFKALSKGTRGSSSGFLNIVMELKKCCNHAFLIKQPEDGENDAPQEHLQSLVRGGGKLVLLDKLLTRLKDRGNRVLIFSQMVRMLDILADYLSMKRYQFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGTVEEDIIERAKKKMVLDHLVIQRMDTTGRTVLDNSSANSNSNPFNKEELSAILKFGAEDLFKEAEGEESEPQEMDIDEILRLAETRESDQGSSATDELLSQFKVANFTMDESTPDLDEKPGRDWDEIIPEEQRRKVEEEQKQKEMEDIYMLPRSRSSNKKAQANDSDSDVGSKLKHRSSGSDSETDDSEDDKRPKRRGRPRARKNNVEGFTDAEIRRFIKAYKKFGAPLERLEAIARDSELVEKSIADLKRLGELVHNSCVTAVQEHEEHLKENPVEAKGPGKRRGINIKISGVQVNAKSIIQHEEEFEPLHKAVPSNPAERNKFQLTCRVKTPHFDVDWDIHDDTQLLLGIYEHGYGNWDLIKTDPDLKLSEKILLDDPEKKPQGKQLQMRTDYLLKMLKKDLESQDAIKAGDEPKIKKRKPRMKKKAPSNELVNEIASPRLSDNPSEEGEVKDDGAEKTPTKKKQKKDNKENKEKTGTPKKEKEGDKDKKRSKPKKEKAKPAGKGKKAQGPVHITQGSEPIPIGEEDDELDQETFSICKERMRPVKKALKQLDKPDEGLSVQDQLQHTRTCLLKIGDRITECLKSYSDPEHIKIWRRNLWIFVSKFTEFGAQKLHRLYKKAQKKRSQEGEKEHKKKDDPIKKKPFRPEPSGSSRDSTGAQPTTKPHPPVSHQSGPPHPPHHREQYNQHNKRHFPNDDRGDWQRDRKYGYPGNSNQPWQGDRHHPYEPHRYKDHHYSDRRPHGDYRSSGGYRNSGSPRKRPYDQYGNDRDHRGHRDYYDRHPDPKRRRSDEYRSPNYHQGGGGGGGGGGGGGHPQDFRRMPDHRGPPGNHGPMGPDHYRPFHPDKPPPLIDPRSPQSQKSPLEPISPGMDRSAEQKSATDFNWNNRKT

Target ORF information:

RefSeq Version	XM_009293623.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 2 (chd2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293623.3

ORF Insert Sequence:

ATGATGAAGA ATAAGAGTAA AAAGCAAGAA GACGAAGGCT CGACTCAAAG CAATGCTTCC 
AGCAACTCAG CATCCGAGGA ATCCAACCGC TCTGGGTCGG AGTCTGGAAG TCAGTCGGAG 
AGCGAGCAGG GCAGTGAAAA AGCACAATCC CGGCATTCTG AATCCAACAG CACGTCTGAT 
TCTGAGAGCC ACTCTGAGTC AGGCAGCGAA TCCAATGGGT CCAAATCTCA TCAGAGCTCA 
GCGCAGGTCA AAGACAAACC TGTCAGGAAG AAAGACAACC TGGCGGATGT GAAGAAGATG 
TGGGAAGAAC ATCCTGATGT TTATGGTGTG AGAAGATCCA GTCGCAGTAG ACAAGAGCCT 
GCACGCCTTA ATATTGGAGC TGAGGGCAGC AGTGATTCAG AGGGAGAAAG TTCCAAACGA 
AAAAGCTCTC GGCAGAAAAA GAAAGAAAAT ACCTGGAAAG ATGATGACTC AAATGACGAA 
GAGGATGTTG AGGAAGAGAC CAGCAGTTCA GAGAGTGAGC AGGAAGAGAA AAAAGTTAGA 
TCCAGACGAC TTCCTGCTAG AAGACCACAG ACCAAATCAT CTGCTAGTAA ACAGTCACAA 
AAGGGACGAA AACAGAGAAA GCAGGAGTCC TCAGATGAAG ATGATGACGA TGATGATGAT 
GACGAGGAGG ACACTCCAAA AAGACAAACA CGGCGGCGGG CTGCTACTAA AGTCAGTTAC 
AAAGAGGACC AGAATGATTT TGAGACGGAC TCTGATGACC TTATTGAAAT GGCAGAAGGG 
GCAGAGGAGC AGCAAGATGA TGACAGTGAG ACCATTGAGA AGGTTGTTGA CACTAGGACT 
GGCAAGAAAG GAGCTACTGG AGCTTCCACA ACTCTCTATG CCATTGAGGA AAACGGGGAC 
CCTGGAGCAG ACTTTGACCC GGAGAAAGAG GAAGGAGAAA CTCAGTTCCT CATCAAGTGG 
AAAGGCTGGT CCTACATCCA CAACACCTGG GAGAGTGTGG ATTCCCTCAC TCAGCAGAAA 
GTCAAAGGCA TGAAGAAGCT GGAAAACTTC AAGAAGAAAA ATGAAGAGCT TAATGCCTGG 
TTGAAAAAAG CATCCCCTGA GGATTTAGAA TATTACAACT GTCAGCAAGA GCTCACAAAC 
GACCTGAGCA AGCAGTTCCA GATAATAGAG AGGGTGATTG CAACAAGAAC AGGCAAATCA 
CAAGCAACTT CAGACACTCC TTCTCATAAA AGCAGCTCTG GTGAGCCCGA GTACCTGTGT 
AAATGGATGG GTCTGCCGTA CGCTGAGTGC ACATGGGAGG ACAGCGCTTT AATCAAAAAG 
AAATTTCAGG CCTGCATTGA CAGTTTCCAC AACAGAAACA GCTCCAAGAC TGTCCCTTCC 
AAAGACTGTA AGGTGTTGAA GCAGAGGCCA AGATTTGTGC CCTTAAAGAA GCAGCCATCG 
TACATTGGAG ATGAGAATCT TGAACTCAGA GATTACCAGC TAGATGGAGT GAACTGGCTG 
GCACACTCCT GGTGCAGGTG TAACAGTGTT ATCTTGGCTG ATGAAATGGG TTTAGGAAAG 
ACCATTCAGA CGATCTCCTT CCTCTCCTAC CTTTTTCATC AGCACCAACT TTACGGCCCC 
TTCATCGTGG TGGTGCCCCT GTCGACCCTC ACGTCCTGGC AGAGGGAGTT TGACACTTGG 
GCCCCGGACA TGAATGTGGT GGTGTATCTG GGAGATGTCA CAAGTAGAAA GACAATCCGA 
GACTATGAAT GGATCAATCC ACAGACAAAA AGGATCAAGT TTAATGCACT TTTGACCACA 
TATGAAATTC TACTGAAGGA CAAGGGAGTG CTGGGGAATA TAAACTGGGC TTTCCTTGGA 
GTAGATGAAG CTCACCGGCT TAAGAATGAC GACTCGCTGC TGTATAAAAC TCTGATCGAC 
TTTCGATCCA ATCACAGGCT GCTTATTACC GGGACTCCTC TGCAAAACTC CCTCAAAGAG 
CTGTGGTCCC TTCTGCACTT TCTGATGTCT GACAAGTTTG AGTCCTGGGA GGATTTTGAG 
GATGAGCATG GTAAAGGCAG GGATAATGGT TATCAGAGTT TGCACAAAGT TCTAGAGCCT 
TTCCTGCTAC GTCGTGTCAA GAAAGATGTG GAGAAATCCC TGCCGGCTAA AGTGGAGCAA 
ATCCTCAGAG TCGACATGTC AGCCCAGCAA AAACAGTTCT ACAAATGGAT CCTAACAAGA 
AACTTCAAGG CCTTGTCTAA AGGCACTAGA GGCAGCTCTT CTGGCTTTCT GAACATTGTC 
ATGGAGCTGA AGAAGTGTTG TAACCACGCT TTCCTCATCA AACAACCAGA AGATGGAGAA 
AATGATGCTC CACAAGAGCA TCTACAGAGT TTGGTGCGTG GTGGAGGGAA ACTGGTTCTC 
CTAGACAAGC TTCTCACACG ACTGAAGGAC AGAGGCAACC GTGTGCTTAT CTTCTCTCAG 
ATGGTCCGCA TGTTGGACAT TCTTGCTGAT TACTTGTCTA TGAAACGATA TCAATTCCAG 
CGTTTGGACG GATCCATCAA GGGTGAGCTC CGAAAACAAG CATTGGACCA CTTTAATGCA 
GAAGGCTCTG AGGACTTCTG TTTCCTGCTG TCCACCCGAG CCGGAGGGCT TGGGATTAAC 
TTGGCCTCTG CTGACACAGT GGTGATCTTT GACTCTGACT GGAACCCACA GAATGATCTT 
CAAGCTCAGG CCAGAGCCCA CAGGATTGGC CAGAAAAAGC AAGTGAATAT CTATCGTCTG 
GTCACAAAAG GGACTGTGGA GGAGGACATT ATTGAAAGAG CTAAAAAGAA GATGGTTCTA 
GACCATCTTG TCATTCAGAG AATGGACACC ACTGGTCGAA CCGTACTGGA TAACAGTTCA 
GCAAATTCAA ATTCAAATCC CTTCAATAAG GAAGAACTGT CAGCGATCTT GAAGTTTGGA 
GCAGAGGATC TGTTTAAGGA AGCTGAGGGG GAGGAATCAG AACCGCAGGA AATGGACATC 
GATGAGATAT TGCGGCTTGC TGAAACCAGA GAGAGCGATC AGGGATCCAG TGCTACCGAT 
GAGCTTCTGT CTCAGTTTAA GGTGGCTAAC TTCACCATGG ATGAAAGCAC ACCAGATCTT 
GATGAAAAAC CTGGCCGGGA CTGGGATGAA ATAATTCCAG AAGAGCAGCG GCGGAAGGTG 
GAGGAAGAGC AGAAGCAGAA GGAGATGGAG GATATCTACA TGCTGCCAAG AAGCAGAAGC 
TCCAACAAGA AGGCACAAGC CAATGACAGC GACAGTGATG TGGGATCCAA ACTAAAGCAC 
AGATCCTCAG GTTCAGACAG TGAAACCGAT GACAGCGAGG ATGACAAGCG GCCTAAGAGG 
CGAGGAAGGC CAAGAGCCCG CAAAAACAAC GTGGAGGGAT TTACTGATGC TGAGATCCGC 
AGGTTTATAA AAGCATACAA GAAATTTGGA GCTCCACTTG AGAGATTGGA GGCGATTGCC 
CGTGACTCTG AGCTGGTGGA GAAGTCCATT GCAGACCTGA AGCGACTAGG GGAGCTGGTG 
CACAACAGCT GCGTGACTGC AGTACAGGAG CACGAAGAGC ACTTGAAGGA AAACCCTGTG 
GAAGCTAAAG GCCCAGGGAA AAGACGAGGC ATTAACATAA AAATCTCTGG AGTTCAGGTC 
AATGCTAAAT CAATTATCCA GCATGAAGAG GAGTTTGAGC CCCTGCATAA AGCTGTGCCT 
TCAAATCCTG CTGAAAGGAA CAAATTTCAG TTGACCTGCC GGGTGAAAAC TCCTCACTTT 
GATGTGGACT GGGATATTCA CGATGACACA CAGTTGTTAT TGGGAATATA TGAGCACGGC 
TACGGCAACT GGGACTTGAT CAAGACTGAT CCTGACCTCA AACTTTCAGA GAAGATTCTT 
CTTGATGACC CTGAAAAGAA ACCTCAAGGA AAGCAGTTAC AGATGCGAAC AGACTACTTG 
CTGAAGATGC TGAAGAAAGA CCTGGAAAGC CAAGATGCCA TTAAAGCAGG AGATGAGCCC 
AAAATTAAAA AGAGAAAACC TCGGATGAAG AAGAAGGCCC CTAGCAATGA ACTGGTCAAT 
GAAATCGCTT CTCCTCGTCT ATCCGACAAC CCATCAGAAG AGGGAGAGGT AAAGGATGAT 
GGAGCTGAAA AAACACCCAC AAAGAAGAAA CAGAAAAAGG ACAACAAAGA GAACAAAGAA 
AAAACAGGGA CTCCTAAAAA AGAGAAGGAG GGGGACAAAG ACAAAAAGCG TTCCAAGCCC 
AAAAAAGAAA AGGCCAAACC AGCAGGAAAA GGGAAGAAAG CCCAGGGCCC TGTGCACATC 
ACTCAAGGAA GTGAACCTAT ACCAATTGGA GAGGAAGATG ATGAGCTGGA CCAGGAAACA 
TTTAGCATTT GTAAGGAGCG GATGCGGCCA GTGAAGAAAG CTCTTAAGCA GTTGGATAAG 
CCAGACGAGG GGCTGTCGGT GCAGGACCAG CTGCAGCACA CACGTACCTG TCTGCTGAAG 
ATCGGAGACC GCATTACAGA GTGCCTTAAG TCATATAGTG ACCCCGAGCA CATCAAGATC 
TGGCGCAGAA ACCTCTGGAT ATTTGTGTCC AAATTTACTG AGTTTGGTGC GCAGAAGCTG 
CATAGGCTAT ATAAGAAGGC TCAGAAGAAG CGCTCCCAGG AGGGAGAGAA AGAGCATAAG 
AAAAAAGACG ATCCTATTAA GAAAAAGCCC TTTAGGCCTG AACCGTCTGG ATCTAGCAGG 
GATTCAACGG GTGCCCAGCC TACAACCAAA CCCCATCCCC CTGTCAGCCA TCAATCTGGA 
CCTCCTCATC CTCCACACCA TAGAGAACAG TACAACCAGC ATAATAAGAG GCATTTTCCT 
AATGATGACA GGGGAGATTG GCAAAGGGAC CGCAAATATG GCTACCCAGG TAACAGCAAT 
CAGCCCTGGC AAGGAGACCG TCATCACCCT TATGAGCCTC ACAGGTACAA GGATCATCAC 
TACAGTGACC GTCGTCCTCA TGGTGACTAC CGCAGCTCTG GAGGATACCG TAACAGTGGC 
TCCCCTCGCA AACGGCCCTA TGACCAGTAT GGCAACGATA GAGATCATCG GGGTCATCGA 
GACTATTATG ACAGACATCC GGACCCAAAG AGAAGGCGCT CGGATGAATA TCGCTCTCCA 
AACTACCACC AAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGAGGAGG GCATCCGCAG 
GACTTTAGAA GAATGCCTGA CCACAGGGGA CCTCCTGGAA ACCATGGCCC CATGGGGCCG 
GATCACTACC GGCCCTTTCA CCCAGACAAA CCCCCGCCTC TGATTGACCC ACGTTCTCCG 
CAGTCTCAGA AATCGCCTCT TGAGCCGATC TCACCTGGTA TGGACAGAAG TGCAGAGCAG 
AAATCTGCGA CAGACTTTAA CTGGAACAAC AGGAAGACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rescue of neural crest-derived phenotypes in a zebrafish CHARGE model by Sox10 downregulation.
Human molecular genetics25(16)3539-3554(2016 08)
Asad Z,Pandey A,Babu A,Sun Y,Shevade K,Kapoor S,Ullah I,Ranjan S,Scaria V,Bajpai R,Sachidanandan C

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

De novo loss-of-function mutations in CHD2 cause a fever-sensitive myoclonic epileptic encephalopathy sharing features with Dravet syndrome.
American journal of human genetics93(5)967-75(2013 Nov)
Suls A,Jaehn JA,Kecsk?s A,Weber Y,Weckhuysen S,Craiu DC,Siekierska A,Dj?mi? T,Afrikanova T,Gormley P,von Spiczak S,Kluger G,Iliescu CM,Talvik T,Talvik I,Meral C,Caglayan HS,Giraldez BG,Serratosa J,Lemke JR,Hoffman-Zacharska D,Szczepanik E,Barisic N,Komarek V,Hjalgrim H,M?ller RS,Linnankivi T,Dimova P,Striano P,Zara F,Marini C,Guerrini R,Depienne C,Baulac S,Kuhlenb?umer G,Crawford AD,Lehesjoki AE,de Witte PA,Palotie A,Lerche H,Esguerra CV,De Jonghe P,Helbig I,

Consequences of lineage-specific gene loss on functional evolution of surviving paralogs: ALDH1A and retinoic acid signaling in vertebrate genomes.
PLoS genetics5(5)e1000496(2009 May)
Ca?estro C,Catchen JM,Rodr?guez-Mar? A,Yokoi H,Postlethwait JH

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chd2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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