Chd1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chd1
Entrez Gene ID	33505
Full Name	Chromodomain-helicase-DNA-binding protein 1
Synonyms	2,CG3733,CHD-1,CHD1,DmCHD1,Dmel\CG3733,chd1,dCHD1,l(2)23Cd
General protein information	Preferred Names Chromodomain-helicase-DNA-binding protein 1 Names CG3733 gene product from transcript CG3733-RC CG3733-PA CG3733-PB CG3733-PC Chd1-PA Chd1-PB Chd1-PC Chromodomain-helicase-DNA-binding protein chromo-ATPase/helicase-DNA-binding domain chromo-ATPase/helicase-DNA-binding protein 1 lethal (2) 23Cd
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23C1-23E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057849.5	NP_477197.1	Chromodomain-helicase-DNA-binding protein 1, isoform A
NM_001258922.1	NP_001245851.1	Chromodomain-helicase-DNA-binding protein 1, isoform B
NM_001273046.1	NP_001259975.1	Chromodomain-helicase-DNA-binding protein 1, isoform C

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CHD2	XP_413879.3
Danio rerio (zebrafish)	chd2	XP_005159227.1
Oryza sativa (rice)	Os07g0660200	NP_001060530.1
Canis lupus familiaris (dog)	CHD2	XP_005618381.1
Mus musculus (house mouse)	Chd2	NP_001074814.2
Caenorhabditis elegans (roundworm)	chd-1	NP_491994.2
	CHD2	XP_002808499.1
Rattus norvegicus (Norway rat)	Chd2	NP_001100993.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008698	XP_314800.4
Homo sapiens (human)	CHD2	NP_001262.3
Drosophila melanogaster (fruit fly)	Chd1	NP_477197.1
Xenopus tropicalis (tropical clawed frog)	chd2	XP_002933353.1
Pan troglodytes (chimpanzee)	CHD2	XP_001170676.2
Arabidopsis thaliana (thale cress)	CHR5	NP_178970.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Drosophila melanogaster CHD1 chromatin remodeling factor modulates global chromosome structure and counteracts HP1a and H3K9me2.
Title: The Drosophila melanogaster CHD1 chromatin remodeling factor modulates global chromosome structure and counteracts HP1a and H3K9me2.

our results emphasize a role for Chd1 in histone replacement in both budding yeast and Drosophila melanogaster, and surprisingly they show that the major effects of Chd1 on turnover occur at the 3' ends of genes.
Title: A key role for Chd1 in histone H3 dynamics at the 3' ends of long genes in yeast.

Intestinal resistance against infection by P. aeruginosa is linked to maintaining proper balance of gut-microbe interactions and the chromatin remodeler CHD1 is involved in regulating this aspect.
Title: CHD1 contributes to intestinal resistance against infection by P. aeruginosa in Drosophila melanogaster.

These results clarify the roles of chromatin remodelers in transcription and provide novel insights into CHD1 function.
Title: Investigations of CHD1 function in transcription and development of Drosophila melanogaster.

findings establish CHD1 as a major factor in replacement histone metabolism in the nucleus and reveal a critical role for CHD1 in the earliest developmental instances of genome-scale, replication-independent nucleosome assembly.
Title: CHD1 motor protein is required for deposition of histone variant H3.3 into chromatin in vivo.

The following Chd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13583	NM_057849.5 Latest version!	Drosophila melanogaster Chromodomain-helicase-DNA-binding protein 1 (Chd1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa16137	NM_001273046.1 Latest version!	Drosophila melanogaster Chromodomain-helicase-DNA-binding protein 1 (Chd1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa16138	NM_001258922.1 Latest version!	Drosophila melanogaster Chromodomain-helicase-DNA-binding protein 1 (Chd1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chd1 ( NM_057849.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477197.1 Drosophila melanogaster Chromodomain-helicase-DNA-binding protein 1 (Chd1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13583
Clone ID Related Accession (Same CDS sequence)	NM_057849.5
Accession Version	NM_057849.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Chromodomain-helicase-DNA-binding protein 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057849.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCCAGG CACTCAATGA ATCGGCGAAT AGTATTGGCT CAGACGAACA AGATGACACT 
CGGGAAGAGG CCAATGGCAC CGACCATAGT GGCAGTGGCA GCGGTAGTGG CTCCTCCGGC 
TCAGACTCAG ATTCAGACAG CTCCTCGGGC AACTCTAGCG ATGGACGGAG CTCCCCAGAA 
CCCGAGGACA AATCGTTATC GGTGGCAGGG TTTCCACCGA CAGCCGCTGC TGCCCAGGCG 
GACAGTAAGA CGAACGGGTT CACGGATGAC CAGGAGGACA GCTCCAGTGA CGGGTCTAGT 
GGCAGTGATT CCGATTCAGA CGCCGAGGGC CCATCGGATC AAAGGAACCA GAGCATCAAC 
AATGCCAACA CAAGCAGCAG TTTGCCTAAG CCGGAGCAGA ATGAGGAGGA GGACAATGAG 
ACTGAGGCTG GTCAGCAGCA GCCAGCCAGC GATGCCTCTG CCGATGAATC AAGTGATAGT 
TCTGCCAATG TGTCACCCAC ATCCTCCAGC AGCAGCAGTG AGGAGGAAGA GGAGGACTAT 
AGACCCAAGC GGACGCGCCA GGCGCGCAAA CCACCGACTG CTGCTGAGAA GTCGAAAAAA 
GCTCCAGCAC CCAAGAACAA GAAGAAAACC TGGGACTCGG ATGAAAGCGA TGAAAGCGAG 
GACAGTGATG ACGAGGTGTC CACCGCCCAA AAGCGCAAAC CGGCAGCAAC TACCTCTCGC 
AGCAAACTCG CACAGCAACA GCAGCGGCGC AGGGTGAAAC CCTTTAGTTC CGAGGACAGT 
GATGATGACG ACGCTAGCAA ACGCTGCGCT ACTCGTCGCA AGGGTGCTGC CGTTAGCTAC 
AAGGAGGCTT CGGAGGACGA AGCCACCGAC TCCGAGGATC TGCTGGAGTT TGAGTACGAT 
GAAAGCCAGG CAGCCACCAC TGCCGCTACG GCTGAGGAGG AGGAAAAGTG CGAAACCATA 
GAGCGTATCC TGGCGCAGCG TGCTGGGAAG AGGGGATGCA CGGGCAATCA GACAACGATC 
TATGCAATAG AGGAAAACGG CTTTGATCCA CATGCTGGAT TTGATGAAAA GCAAACTCCA 
GACGCGGAGA CCGAGGCACA GTTCCTGATC AAGTGGAAGG GCTGGTCGTA CATCCACAAC 
ACCTGGGAAT CTGAGGCTAC CCTGCGTGAT ATGAAGGCCA AGGGAATGAA GAAGCTGGAT 
AACTTCATCA AAAAGGAGAA GGAACAGGCC TACTGGCGCC GATACGCTGG CCCCGAGGAC 
ATCGACTACT TCGAGTGTCA GCTGGAACTG CAGCATGAGC TACTGAAATC GTACAACAAC 
GTGGACCGAA TCATTGCCAA GGGCTCTAAG CCAGACGACG GCACCGAGGA GTACCTGTGC 
AAATGGCAGT CGCTACCGTA CGCCGAGTCC ACATGGGAGG ATGCCGCCCT GGTTCTTCGC 
AAGTGGCAAC GCTGCGCAGA GCAATTCAAT GACCGCGAGT CCTCCAAATG CACTCCCTCC 
CGCCACTGCA GAGTGATCAA GTATCGCCCC AAGTTCTCTC GAATAAAGAA TCAGCCGGAA 
TTTCTTTCGT CGGGCCTAAC TCTAAGGGAT TACCAAATGG ACGGCCTGAA TTGGCTGCTG 
CACTCATGGT GCAAGGAGAA CTCGGTGATC CTGGCCGACG AGATGGGCCT CGGCAAGACC 
ATCCAAACTA TTTGCTTCCT GTACTCGCTG TTCAAGATCC ATCACCTGTA CGGCCCGTTC 
CTGTGCGTGG TGCCACTTAG CACAATGACG GCCTGGCAGC GGGAGTTCGA CCTGTGGGCG 
CCGGACATGA ATGTAGTCAC GTACCTCGGT GACATTAAAT CCCGCGAGCT CATTCAACAG 
TACGAATGGC AGTTTGAGAG CTCCAAGCGG CTTAAATTCA ATTGCATTCT CACAACGTAC 
GAGATTGTGC TCAAGGATAA ACAATTTCTG GGCACGCTTC AGTGGGCTGC GCTCCTGGTG 
GACGAAGCGC ATCGTCTTAA GAACGACGAC TCACTGCTCT ACAAGTCGCT GAAGGAGTTT 
GACACTAACC ATCGACTGCT CATAACAGGT ACCCCGCTGC AGAATTCGCT GAAGGAGCTC 
TGGGCTTTGC TGCACTTCAT CATGCCGGAT AAGTTTGACA CGTGGGAGAA TTTCGAGGTG 
CAGCATGGCA ATGCGGAGGA CAAGGGCTAC ACTCGGTTGC ATCAACAGCT GGAGCCGTAT 
ATTCTGCGGA GAGTGAAGAA GGATGTGGAG AAATCGCTGC CGGCTAAAGT GGAGCAGATC 
CTGCGGGTCG AGATGACCTC TCTGCAGAAA CAGTACTACA AGTGGATCCT AACCAAAAAC 
TTTGATGCAC TGCGCAAGGG AAAGCGGGGC AGTACCTCCA CTTTCCTCAA CATAGTCATC 
GAGCTAAAGA AGTGCTGCAA CCACGCGGCT CTCATCCGAC CATCCGAGTT TGAGCTTATG 
GGTCTGCAGC AGGATGAGGC ATTGCAAACT CTTCTTAAGG GATCCGGAAA GCTTGTGCTG 
CTCGATAAGC TACTCTGCCG ACTGAAAGAG ACGGGCCACC GCGTGCTGAT TTTTTCCCAA 
ATGGTGCGCA TGCTGGACGT TCTGGCTGAC TATCTGCAGA AGCGTCACTT CCCTTTCCAG 
CGCCTCGACG GCAGCATAAA GGGAGAGATG AGGCGCCAGG CTCTGGATCA CTTCAATGCA 
GAGGGTAGCC AGGACTTTTG CTTCCTGCTT TCGACAAGGG CTGGTGGATT GGGCATTAAC 
TTGGCCACAG CCGATACTGT TATCATCTTT GACTCCGACT GGAACCCCCA AAACGATTTA 
CAGGCGCAGG CGAGAGCGCA TCGAATCGGT CAGAAAAACC AGGTTAACAT TTATCGCCTG 
GTCACCGCCC GGTCGGTGGA GGAACAGATC GTGGAGCGAG CCAAACAGAA AATGGTACTG 
GACCACCTGG TCATTCAGCG GATGGACACT ACAGGACGCA CTGTTCTGGA TAAGAGCGGC 
AACGGGCACT CGTCTAACTC GAATCCGTTC AACAAGGACG ATCTTTCTGC TATCCTGAAG 
TTTGGTGCAG AGGAGCTGTT CAAGGACGAA CAGGAGCATG ACGACGACTT GGTTTGTGAC 
ATTGATGAAA TTCTGCGCAG GGCGGAGACC CGCAACGAAG ATCCGGAAAT GCCAGCAGAC 
GACTTGTTGT CCGCCTTCAA AGTGGCCAGC ATAGCTGCTT TCGAAGAGGA ACCGAGCGAT 
TCGGTTAGCA AGCAAGACCA AAATGCCGCC GGCGAGGAGG ATGACAGTAA GGACTGGGAC 
GACATCATTC CGGAAGGCTT CCGTAAAGCA ATCGACGATC AGGAGCGAGC AAAAGAGATG 
GAAGATCTCT ACTTGCCACC CCGGAGGAAA ACTGCCGCTA ACCAAAACGA AGGGAAGCGT 
GGAGCTGGCA AGGGGGGCAA GGGGAAACAG CAGGCAGACG ACTCCGGTGG CGACTCGGAT 
TACGAGCTGG GCTCCGACGG CAGTGGTGAC GATGGACGAC CTCGCAAGCG CGGGCGGCCG 
ACCATGAAGG AGAAGATCAC CGGATTCACT GATGCGGAGT TGCGTCGCTT CATTCGCAGT 
TACAAAAAGT TTCCCGCTCC TCTCCACCGC ATGGAGGCCA TCGCATGTGA TGCTGAGCTG 
CAGGAAAAGC CACTGGCGGA ACTGAAGCGC CTCGGAGAGA TGCTGCATGA CCGCTGCGTT 
CAGTTCCTGC ACGAGCACAA GGAGGAAGAG AGTAAGACTG CGGCGACGGA TGAGACGCCG 
GGCGCCAAGC AGCGCCGCGC ACGCGCCACC TTCTCCGTTA AGCTGGGTGG CGTCTCCTTT 
AATGCCAAAA AGCTGCTGGC CTGCGAACAG GAGCTTCAGC CGCTCAACGA GATCATGCCC 
AGCATGCCCG AAGAGCGCCA GCAATGGAGC TTCAATATCA AGACACGCGC CCCGGTCTTC 
GACGTGGATT GGGGCATCGA GGAGGACACG AAGCTACTGT GCGGCATTTA CCAGTACGGC 
ATTGGATCCT GGGAGCAGAT GAAGCTAGAC CCCACGCTCA AACTGACCGA CAAGATTTTG 
CTTAACGATA CGCGCAAGCC CCAGGCCAAG CAGCTGCAGA CGCGTGCCGA GTACCTGCTC 
AAGATCATCA AGAAGAACGT GGAGCTGACC AAGGGAGGAC AACGCCGACA ACGGCGCCCC 
AGAGCATCGC GAGCTAACGA TGCCAAGGCG GCAAGCCAGT CTGCTAGTTC GACCATCGAT 
GCTAAGCCGC ACGATGGCGA GGACGCTGCT GGCGACGCAC GCACCGTCGC CGAAAGCAGT 
AACAGTCAAG TAGATCCATC TACCGCGTCG CCGCACAATG CTCCAGCTAC GGAACAGCAC 
GGTGACCCTG CGAAGAAAGC CAAAAAATCC AAGGCTCGTT CGAAGAAAAC CAGCGCCTCG 
GACAACAACG GCAACAAGCC GATGCACTTT ACGGCGAACA ATGAACCTAG GGCTTTGGAG 
GTCCTGGGTG ACCTAGATCC CAGCATTTTT AACGAGTGCA AAGAAAAAAT GCGGCCGGTG 
AAGAAGGCCC TCAAGGCGCT GGACCAGCCG GATGTCAGTC TTTCCGACCA GGATCAGCTG 
CAGCACACGA GGGATTGCCT GCTTCAAATT GGCAAGCAGA TCGATGTTTG CCTCAATCCC 
TACGCCGAGA CAGAGAAAAA GGAATGGCGC AGTAACCTTT GGTACTTTGT GTCGAAATTC 
ACTGAGCTGG ATGCCAAGCG CCTATTCAAG ATTTACAAAC ACGCACTCAA GCAGAAAGCG 
GGCGGCGATG GCGAGGCGAA GGGAAAAGAC AAGGGCTCGT CAGGAAGTCC GGCCAAGTCT 
AAACCCAACG GTGTGACTAC TGAAGAAAAA GAAAAGGAGC GCGACAGGAG TGGGGGCAAA 
AAGAAGAAAA AGGACAAGGA CAAGGAACGC AGTGGACAGG CGCGGTATCC GGAAACGGGT 
ATACCCACCT CCGGTCGTTA CGCTGACCCT CCCCTAAAGC GTAAGCGGGA TGAGAACGAC 
GCTGATGCAA GCAGCGGACT AGCAGGTGCC CCCGGCGGAG GCATCGGCGA CAACCTCAAG 
TCCATGTCCT TCAAACGGCT GAACATGGAC CGTCACGAGG ACCGTAAGAA GCACCATCGC 
GGTCCCGACT ACTACGGTGG CAGTGGCCCA CCCATGGGAA GTGGTAGCTA CGAAGGCGGC 
AGCAATTCCC GGCGCCAGGG CCCCACCTCC CCATCTACAC CCCGTACTGG CCGGGGCGGC 
TATGATCCAC CGCCCGCGCC ATCCGGCTAC ACGCCGGAAA TGGAGCGATG GCAATCCCGC 
GATAGATACA GCCAGGATTA CAAACGGGAT CGCTACGATG GATATGGGCG CAGTGGGGGA 
GGTCAAGGCA GCTATCATCG CGAACGCGAC CGTCGTCCCG AGAAGCGCAG ATATCCGTCT 
GGCTTGCCAC CGCATCCTTA CTCAAGCCAC TACCTGCCGC CCAATTACTA TGGATTGCCT 
AATGGAGCCG TTCCGGGCCT ACCGCCACCG TCGTCTGTCT ACCGCAGTGA CCCACGCGGA 
TATCCGGTCA TGCCGCGAGA CTATCCTGCC GACTACAGAC GCAGCGACTA CGAGCGGCGC 
ACGCAGACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477197.1
CDS	204..5855
Translation	MSQALNESANSIGSDEQDDTREEANGTDHSGSGSGSGSSGSDSDSDSSSGNSSDGRSSPEPEDKSLSVAGFPPTAAAAQADSKTNGFTDDQEDSSSDGSSGSDSDSDAEGPSDQRNQSINNANTSSSLPKPEQNEEEDNETEAGQQQPASDASADESSDSSANVSPTSSSSSSEEEEEDYRPKRTRQARKPPTAAEKSKKAPAPKNKKKTWDSDESDESEDSDDEVSTAQKRKPAATTSRSKLAQQQQRRRVKPFSSEDSDDDDASKRCATRRKGAAVSYKEASEDEATDSEDLLEFEYDESQAATTAATAEEEEKCETIERILAQRAGKRGCTGNQTTIYAIEENGFDPHAGFDEKQTPDAETEAQFLIKWKGWSYIHNTWESEATLRDMKAKGMKKLDNFIKKEKEQAYWRRYAGPEDIDYFECQLELQHELLKSYNNVDRIIAKGSKPDDGTEEYLCKWQSLPYAESTWEDAALVLRKWQRCAEQFNDRESSKCTPSRHCRVIKYRPKFSRIKNQPEFLSSGLTLRDYQMDGLNWLLHSWCKENSVILADEMGLGKTIQTICFLYSLFKIHHLYGPFLCVVPLSTMTAWQREFDLWAPDMNVVTYLGDIKSRELIQQYEWQFESSKRLKFNCILTTYEIVLKDKQFLGTLQWAALLVDEAHRLKNDDSLLYKSLKEFDTNHRLLITGTPLQNSLKELWALLHFIMPDKFDTWENFEVQHGNAEDKGYTRLHQQLEPYILRRVKKDVEKSLPAKVEQILRVEMTSLQKQYYKWILTKNFDALRKGKRGSTSTFLNIVIELKKCCNHAALIRPSEFELMGLQQDEALQTLLKGSGKLVLLDKLLCRLKETGHRVLIFSQMVRMLDVLADYLQKRHFPFQRLDGSIKGEMRRQALDHFNAEGSQDFCFLLSTRAGGLGINLATADTVIIFDSDWNPQNDLQAQARAHRIGQKNQVNIYRLVTARSVEEQIVERAKQKMVLDHLVIQRMDTTGRTVLDKSGNGHSSNSNPFNKDDLSAILKFGAEELFKDEQEHDDDLVCDIDEILRRAETRNEDPEMPADDLLSAFKVASIAAFEEEPSDSVSKQDQNAAGEEDDSKDWDDIIPEGFRKAIDDQERAKEMEDLYLPPRRKTAANQNEGKRGAGKGGKGKQQADDSGGDSDYELGSDGSGDDGRPRKRGRPTMKEKITGFTDAELRRFIRSYKKFPAPLHRMEAIACDAELQEKPLAELKRLGEMLHDRCVQFLHEHKEEESKTAATDETPGAKQRRARATFSVKLGGVSFNAKKLLACEQELQPLNEIMPSMPEERQQWSFNIKTRAPVFDVDWGIEEDTKLLCGIYQYGIGSWEQMKLDPTLKLTDKILLNDTRKPQAKQLQTRAEYLLKIIKKNVELTKGGQRRQRRPRASRANDAKAASQSASSTIDAKPHDGEDAAGDARTVAESSNSQVDPSTASPHNAPATEQHGDPAKKAKKSKARSKKTSASDNNGNKPMHFTANNEPRALEVLGDLDPSIFNECKEKMRPVKKALKALDQPDVSLSDQDQLQHTRDCLLQIGKQIDVCLNPYAETEKKEWRSNLWYFVSKFTELDAKRLFKIYKHALKQKAGGDGEAKGKDKGSSGSPAKSKPNGVTTEEKEKERDRSGGKKKKKDKDKERSGQARYPETGIPTSGRYADPPLKRKRDENDADASSGLAGAPGGGIGDNLKSMSFKRLNMDRHEDRKKHHRGPDYYGGSGPPMGSGSYEGGSNSRRQGPTSPSTPRTGRGGYDPPPAPSGYTPEMERWQSRDRYSQDYKRDRYDGYGRSGGGQGSYHRERDRRPEKRRYPSGLPPHPYSSHYLPPNYYGLPNGAVPGLPPPSSVYRSDPRGYPVMPRDYPADYRRSDYERRTQT

Target ORF information:

RefSeq Version	NM_057849.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Chromodomain-helicase-DNA-binding protein 1 (Chd1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057849.5

ORF Insert Sequence:

ATGAGCCAGG CACTCAATGA ATCGGCGAAT AGTATTGGCT CAGACGAACA AGATGACACT 
CGGGAAGAGG CCAATGGCAC CGACCATAGT GGCAGTGGCA GCGGTAGTGG CTCCTCCGGC 
TCAGACTCAG ATTCAGACAG CTCCTCGGGC AACTCTAGCG ATGGACGGAG CTCCCCAGAA 
CCCGAGGACA AATCGTTATC GGTGGCAGGG TTTCCACCGA CAGCCGCTGC TGCCCAGGCG 
GACAGTAAGA CGAACGGGTT CACGGATGAC CAGGAGGACA GCTCCAGTGA CGGGTCTAGT 
GGCAGTGATT CCGATTCAGA CGCCGAGGGC CCATCGGATC AAAGGAACCA GAGCATCAAC 
AATGCCAACA CAAGCAGCAG TTTGCCTAAG CCGGAGCAGA ATGAGGAGGA GGACAATGAG 
ACTGAGGCTG GTCAGCAGCA GCCAGCCAGC GATGCCTCTG CCGATGAATC AAGTGATAGT 
TCTGCCAATG TGTCACCCAC ATCCTCCAGC AGCAGCAGTG AGGAGGAAGA GGAGGACTAT 
AGACCCAAGC GGACGCGCCA GGCGCGCAAA CCACCGACTG CTGCTGAGAA GTCGAAAAAA 
GCTCCAGCAC CCAAGAACAA GAAGAAAACC TGGGACTCGG ATGAAAGCGA TGAAAGCGAG 
GACAGTGATG ACGAGGTGTC CACCGCCCAA AAGCGCAAAC CGGCAGCAAC TACCTCTCGC 
AGCAAACTCG CACAGCAACA GCAGCGGCGC AGGGTGAAAC CCTTTAGTTC CGAGGACAGT 
GATGATGACG ACGCTAGCAA ACGCTGCGCT ACTCGTCGCA AGGGTGCTGC CGTTAGCTAC 
AAGGAGGCTT CGGAGGACGA AGCCACCGAC TCCGAGGATC TGCTGGAGTT TGAGTACGAT 
GAAAGCCAGG CAGCCACCAC TGCCGCTACG GCTGAGGAGG AGGAAAAGTG CGAAACCATA 
GAGCGTATCC TGGCGCAGCG TGCTGGGAAG AGGGGATGCA CGGGCAATCA GACAACGATC 
TATGCAATAG AGGAAAACGG CTTTGATCCA CATGCTGGAT TTGATGAAAA GCAAACTCCA 
GACGCGGAGA CCGAGGCACA GTTCCTGATC AAGTGGAAGG GCTGGTCGTA CATCCACAAC 
ACCTGGGAAT CTGAGGCTAC CCTGCGTGAT ATGAAGGCCA AGGGAATGAA GAAGCTGGAT 
AACTTCATCA AAAAGGAGAA GGAACAGGCC TACTGGCGCC GATACGCTGG CCCCGAGGAC 
ATCGACTACT TCGAGTGTCA GCTGGAACTG CAGCATGAGC TACTGAAATC GTACAACAAC 
GTGGACCGAA TCATTGCCAA GGGCTCTAAG CCAGACGACG GCACCGAGGA GTACCTGTGC 
AAATGGCAGT CGCTACCGTA CGCCGAGTCC ACATGGGAGG ATGCCGCCCT GGTTCTTCGC 
AAGTGGCAAC GCTGCGCAGA GCAATTCAAT GACCGCGAGT CCTCCAAATG CACTCCCTCC 
CGCCACTGCA GAGTGATCAA GTATCGCCCC AAGTTCTCTC GAATAAAGAA TCAGCCGGAA 
TTTCTTTCGT CGGGCCTAAC TCTAAGGGAT TACCAAATGG ACGGCCTGAA TTGGCTGCTG 
CACTCATGGT GCAAGGAGAA CTCGGTGATC CTGGCCGACG AGATGGGCCT CGGCAAGACC 
ATCCAAACTA TTTGCTTCCT GTACTCGCTG TTCAAGATCC ATCACCTGTA CGGCCCGTTC 
CTGTGCGTGG TGCCACTTAG CACAATGACG GCCTGGCAGC GGGAGTTCGA CCTGTGGGCG 
CCGGACATGA ATGTAGTCAC GTACCTCGGT GACATTAAAT CCCGCGAGCT CATTCAACAG 
TACGAATGGC AGTTTGAGAG CTCCAAGCGG CTTAAATTCA ATTGCATTCT CACAACGTAC 
GAGATTGTGC TCAAGGATAA ACAATTTCTG GGCACGCTTC AGTGGGCTGC GCTCCTGGTG 
GACGAAGCGC ATCGTCTTAA GAACGACGAC TCACTGCTCT ACAAGTCGCT GAAGGAGTTT 
GACACTAACC ATCGACTGCT CATAACAGGT ACCCCGCTGC AGAATTCGCT GAAGGAGCTC 
TGGGCTTTGC TGCACTTCAT CATGCCGGAT AAGTTTGACA CGTGGGAGAA TTTCGAGGTG 
CAGCATGGCA ATGCGGAGGA CAAGGGCTAC ACTCGGTTGC ATCAACAGCT GGAGCCGTAT 
ATTCTGCGGA GAGTGAAGAA GGATGTGGAG AAATCGCTGC CGGCTAAAGT GGAGCAGATC 
CTGCGGGTCG AGATGACCTC TCTGCAGAAA CAGTACTACA AGTGGATCCT AACCAAAAAC 
TTTGATGCAC TGCGCAAGGG AAAGCGGGGC AGTACCTCCA CTTTCCTCAA CATAGTCATC 
GAGCTAAAGA AGTGCTGCAA CCACGCGGCT CTCATCCGAC CATCCGAGTT TGAGCTTATG 
GGTCTGCAGC AGGATGAGGC ATTGCAAACT CTTCTTAAGG GATCCGGAAA GCTTGTGCTG 
CTCGATAAGC TACTCTGCCG ACTGAAAGAG ACGGGCCACC GCGTGCTGAT TTTTTCCCAA 
ATGGTGCGCA TGCTGGACGT TCTGGCTGAC TATCTGCAGA AGCGTCACTT CCCTTTCCAG 
CGCCTCGACG GCAGCATAAA GGGAGAGATG AGGCGCCAGG CTCTGGATCA CTTCAATGCA 
GAGGGTAGCC AGGACTTTTG CTTCCTGCTT TCGACAAGGG CTGGTGGATT GGGCATTAAC 
TTGGCCACAG CCGATACTGT TATCATCTTT GACTCCGACT GGAACCCCCA AAACGATTTA 
CAGGCGCAGG CGAGAGCGCA TCGAATCGGT CAGAAAAACC AGGTTAACAT TTATCGCCTG 
GTCACCGCCC GGTCGGTGGA GGAACAGATC GTGGAGCGAG CCAAACAGAA AATGGTACTG 
GACCACCTGG TCATTCAGCG GATGGACACT ACAGGACGCA CTGTTCTGGA TAAGAGCGGC 
AACGGGCACT CGTCTAACTC GAATCCGTTC AACAAGGACG ATCTTTCTGC TATCCTGAAG 
TTTGGTGCAG AGGAGCTGTT CAAGGACGAA CAGGAGCATG ACGACGACTT GGTTTGTGAC 
ATTGATGAAA TTCTGCGCAG GGCGGAGACC CGCAACGAAG ATCCGGAAAT GCCAGCAGAC 
GACTTGTTGT CCGCCTTCAA AGTGGCCAGC ATAGCTGCTT TCGAAGAGGA ACCGAGCGAT 
TCGGTTAGCA AGCAAGACCA AAATGCCGCC GGCGAGGAGG ATGACAGTAA GGACTGGGAC 
GACATCATTC CGGAAGGCTT CCGTAAAGCA ATCGACGATC AGGAGCGAGC AAAAGAGATG 
GAAGATCTCT ACTTGCCACC CCGGAGGAAA ACTGCCGCTA ACCAAAACGA AGGGAAGCGT 
GGAGCTGGCA AGGGGGGCAA GGGGAAACAG CAGGCAGACG ACTCCGGTGG CGACTCGGAT 
TACGAGCTGG GCTCCGACGG CAGTGGTGAC GATGGACGAC CTCGCAAGCG CGGGCGGCCG 
ACCATGAAGG AGAAGATCAC CGGATTCACT GATGCGGAGT TGCGTCGCTT CATTCGCAGT 
TACAAAAAGT TTCCCGCTCC TCTCCACCGC ATGGAGGCCA TCGCATGTGA TGCTGAGCTG 
CAGGAAAAGC CACTGGCGGA ACTGAAGCGC CTCGGAGAGA TGCTGCATGA CCGCTGCGTT 
CAGTTCCTGC ACGAGCACAA GGAGGAAGAG AGTAAGACTG CGGCGACGGA TGAGACGCCG 
GGCGCCAAGC AGCGCCGCGC ACGCGCCACC TTCTCCGTTA AGCTGGGTGG CGTCTCCTTT 
AATGCCAAAA AGCTGCTGGC CTGCGAACAG GAGCTTCAGC CGCTCAACGA GATCATGCCC 
AGCATGCCCG AAGAGCGCCA GCAATGGAGC TTCAATATCA AGACACGCGC CCCGGTCTTC 
GACGTGGATT GGGGCATCGA GGAGGACACG AAGCTACTGT GCGGCATTTA CCAGTACGGC 
ATTGGATCCT GGGAGCAGAT GAAGCTAGAC CCCACGCTCA AACTGACCGA CAAGATTTTG 
CTTAACGATA CGCGCAAGCC CCAGGCCAAG CAGCTGCAGA CGCGTGCCGA GTACCTGCTC 
AAGATCATCA AGAAGAACGT GGAGCTGACC AAGGGAGGAC AACGCCGACA ACGGCGCCCC 
AGAGCATCGC GAGCTAACGA TGCCAAGGCG GCAAGCCAGT CTGCTAGTTC GACCATCGAT 
GCTAAGCCGC ACGATGGCGA GGACGCTGCT GGCGACGCAC GCACCGTCGC CGAAAGCAGT 
AACAGTCAAG TAGATCCATC TACCGCGTCG CCGCACAATG CTCCAGCTAC GGAACAGCAC 
GGTGACCCTG CGAAGAAAGC CAAAAAATCC AAGGCTCGTT CGAAGAAAAC CAGCGCCTCG 
GACAACAACG GCAACAAGCC GATGCACTTT ACGGCGAACA ATGAACCTAG GGCTTTGGAG 
GTCCTGGGTG ACCTAGATCC CAGCATTTTT AACGAGTGCA AAGAAAAAAT GCGGCCGGTG 
AAGAAGGCCC TCAAGGCGCT GGACCAGCCG GATGTCAGTC TTTCCGACCA GGATCAGCTG 
CAGCACACGA GGGATTGCCT GCTTCAAATT GGCAAGCAGA TCGATGTTTG CCTCAATCCC 
TACGCCGAGA CAGAGAAAAA GGAATGGCGC AGTAACCTTT GGTACTTTGT GTCGAAATTC 
ACTGAGCTGG ATGCCAAGCG CCTATTCAAG ATTTACAAAC ACGCACTCAA GCAGAAAGCG 
GGCGGCGATG GCGAGGCGAA GGGAAAAGAC AAGGGCTCGT CAGGAAGTCC GGCCAAGTCT 
AAACCCAACG GTGTGACTAC TGAAGAAAAA GAAAAGGAGC GCGACAGGAG TGGGGGCAAA 
AAGAAGAAAA AGGACAAGGA CAAGGAACGC AGTGGACAGG CGCGGTATCC GGAAACGGGT 
ATACCCACCT CCGGTCGTTA CGCTGACCCT CCCCTAAAGC GTAAGCGGGA TGAGAACGAC 
GCTGATGCAA GCAGCGGACT AGCAGGTGCC CCCGGCGGAG GCATCGGCGA CAACCTCAAG 
TCCATGTCCT TCAAACGGCT GAACATGGAC CGTCACGAGG ACCGTAAGAA GCACCATCGC 
GGTCCCGACT ACTACGGTGG CAGTGGCCCA CCCATGGGAA GTGGTAGCTA CGAAGGCGGC 
AGCAATTCCC GGCGCCAGGG CCCCACCTCC CCATCTACAC CCCGTACTGG CCGGGGCGGC 
TATGATCCAC CGCCCGCGCC ATCCGGCTAC ACGCCGGAAA TGGAGCGATG GCAATCCCGC 
GATAGATACA GCCAGGATTA CAAACGGGAT CGCTACGATG GATATGGGCG CAGTGGGGGA 
GGTCAAGGCA GCTATCATCG CGAACGCGAC CGTCGTCCCG AGAAGCGCAG ATATCCGTCT 
GGCTTGCCAC CGCATCCTTA CTCAAGCCAC TACCTGCCGC CCAATTACTA TGGATTGCCT 
AATGGAGCCG TTCCGGGCCT ACCGCCACCG TCGTCTGTCT ACCGCAGTGA CCCACGCGGA 
TATCCGGTCA TGCCGCGAGA CTATCCTGCC GACTACAGAC GCAGCGACTA CGAGCGGCGC 
ACGCAGACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd1 ( NM_001273046.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259975.1 Drosophila melanogaster Chromodomain-helicase-DNA-binding protein 1 (Chd1), transcript variant C, mRNA.

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CloneID	OFa16137
Clone ID Related Accession (Same CDS sequence)	NM_001273046.1
Accession Version	NM_001273046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Chromodomain-helicase-DNA-binding protein 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCCAGG CACTCAATGA ATCGGCGAAT AGTATTGGCT CAGACGAACA AGATGACACT 
CGGGAAGAGG CCAATGGCAC CGACCATAGT GGCAGTGGCA GCGGTAGTGG CTCCTCCGGC 
TCAGACTCAG ATTCAGACAG CTCCTCGGGC AACTCTAGCG ATGGACGGAG CTCCCCAGAA 
CCCGAGGACA AATCGTTATC GGTGGCAGGG TTTCCACCGA CAGCCGCTGC TGCCCAGGCG 
GACAGTAAGA CGAACGGGTT CACGGATGAC CAGGAGGACA GCTCCAGTGA CGGGTCTAGT 
GGCAGTGATT CCGATTCAGA CGCCGAGGGC CCATCGGATC AAAGGAACCA GAGCATCAAC 
AATGCCAACA CAAGCAGCAG TTTGCCTAAG CCGGAGCAGA ATGAGGAGGA GGACAATGAG 
ACTGAGGCTG GTCAGCAGCA GCCAGCCAGC GATGCCTCTG CCGATGAATC AAGTGATAGT 
TCTGCCAATG TGTCACCCAC ATCCTCCAGC AGCAGCAGTG AGGAGGAAGA GGAGGACTAT 
AGACCCAAGC GGACGCGCCA GGCGCGCAAA CCACCGACTG CTGCTGAGAA GTCGAAAAAA 
GCTCCAGCAC CCAAGAACAA GAAGAAAACC TGGGACTCGG ATGAAAGCGA TGAAAGCGAG 
GACAGTGATG ACGAGGTGTC CACCGCCCAA AAGCGCAAAC CGGCAGCAAC TACCTCTCGC 
AGCAAACTCG CACAGCAACA GCAGCGGCGC AGGGTGAAAC CCTTTAGTTC CGAGGACAGT 
GATGATGACG ACGCTAGCAA ACGCTGCGCT ACTCGTCGCA AGGGTGCTGC CGTTAGCTAC 
AAGGAGGCTT CGGAGGACGA AGCCACCGAC TCCGAGGATC TGCTGGAGTT TGAGTACGAT 
GAAAGCCAGG CAGCCACCAC TGCCGCTACG GCTGAGGAGG AGGAAAAGTG CGAAACCATA 
GAGCGTATCC TGGCGCAGCG TGCTGGGAAG AGGGGATGCA CGGGCAATCA GACAACGATC 
TATGCAATAG AGGAAAACGG CTTTGATCCA CATGCTGGAT TTGATGAAAA GCAAACTCCA 
GACGCGGAGA CCGAGGCACA GTTCCTGATC AAGTGGAAGG GCTGGTCGTA CATCCACAAC 
ACCTGGGAAT CTGAGGCTAC CCTGCGTGAT ATGAAGGCCA AGGGAATGAA GAAGCTGGAT 
AACTTCATCA AAAAGGAGAA GGAACAGGCC TACTGGCGCC GATACGCTGG CCCCGAGGAC 
ATCGACTACT TCGAGTGTCA GCTGGAACTG CAGCATGAGC TACTGAAATC GTACAACAAC 
GTGGACCGAA TCATTGCCAA GGGCTCTAAG CCAGACGACG GCACCGAGGA GTACCTGTGC 
AAATGGCAGT CGCTACCGTA CGCCGAGTCC ACATGGGAGG ATGCCGCCCT GGTTCTTCGC 
AAGTGGCAAC GCTGCGCAGA GCAATTCAAT GACCGCGAGT CCTCCAAATG CACTCCCTCC 
CGCCACTGCA GAGTGATCAA GTATCGCCCC AAGTTCTCTC GAATAAAGAA TCAGCCGGAA 
TTTCTTTCGT CGGGCCTAAC TCTAAGGGAT TACCAAATGG ACGGCCTGAA TTGGCTGCTG 
CACTCATGGT GCAAGGAGAA CTCGGTGATC CTGGCCGACG AGATGGGCCT CGGCAAGACC 
ATCCAAACTA TTTGCTTCCT GTACTCGCTG TTCAAGATCC ATCACCTGTA CGGCCCGTTC 
CTGTGCGTGG TGCCACTTAG CACAATGACG GCCTGGCAGC GGGAGTTCGA CCTGTGGGCG 
CCGGACATGA ATGTAGTCAC GTACCTCGGT GACATTAAAT CCCGCGAGCT CATTCAACAG 
TACGAATGGC AGTTTGAGAG CTCCAAGCGG CTTAAATTCA ATTGCATTCT CACAACGTAC 
GAGATTGTGC TCAAGGATAA ACAATTTCTG GGCACGCTTC AGTGGGCTGC GCTCCTGGTG 
GACGAAGCGC ATCGTCTTAA GAACGACGAC TCACTGCTCT ACAAGTCGCT GAAGGAGTTT 
GACACTAACC ATCGACTGCT CATAACAGGT ACCCCGCTGC AGAATTCGCT GAAGGAGCTC 
TGGGCTTTGC TGCACTTCAT CATGCCGGAT AAGTTTGACA CGTGGGAGAA TTTCGAGGTG 
CAGCATGGCA ATGCGGAGGA CAAGGGCTAC ACTCGGTTGC ATCAACAGCT GGAGCCGTAT 
ATTCTGCGGA GAGTGAAGAA GGATGTGGAG AAATCGCTGC CGGCTAAAGT GGAGCAGATC 
CTGCGGGTCG AGATGACCTC TCTGCAGAAA CAGTACTACA AGTGGATCCT AACCAAAAAC 
TTTGATGCAC TGCGCAAGGG AAAGCGGGGC AGTACCTCCA CTTTCCTCAA CATAGTCATC 
GAGCTAAAGA AGTGCTGCAA CCACGCGGCT CTCATCCGAC CATCCGAGTT TGAGCTTATG 
GGTCTGCAGC AGGATGAGGC ATTGCAAACT CTTCTTAAGG GATCCGGAAA GCTTGTGCTG 
CTCGATAAGC TACTCTGCCG ACTGAAAGAG ACGGGCCACC GCGTGCTGAT TTTTTCCCAA 
ATGGTGCGCA TGCTGGACGT TCTGGCTGAC TATCTGCAGA AGCGTCACTT CCCTTTCCAG 
CGCCTCGACG GCAGCATAAA GGGAGAGATG AGGCGCCAGG CTCTGGATCA CTTCAATGCA 
GAGGGTAGCC AGGACTTTTG CTTCCTGCTT TCGACAAGGG CTGGTGGATT GGGCATTAAC 
TTGGCCACAG CCGATACTGT TATCATCTTT GACTCCGACT GGAACCCCCA AAACGATTTA 
CAGGCGCAGG CGAGAGCGCA TCGAATCGGT CAGAAAAACC AGGTTAACAT TTATCGCCTG 
GTCACCGCCC GGTCGGTGGA GGAACAGATC GTGGAGCGAG CCAAACAGAA AATGGTACTG 
GACCACCTGG TCATTCAGCG GATGGACACT ACAGGACGCA CTGTTCTGGA TAAGAGCGGC 
AACGGGCACT CGTCTAACTC GAATCCGTTC AACAAGGACG ATCTTTCTGC TATCCTGAAG 
TTTGGTGCAG AGGAGCTGTT CAAGGACGAA CAGGAGCATG ACGACGACTT GGTTTGTGAC 
ATTGATGAAA TTCTGCGCAG GGCGGAGACC CGCAACGAAG ATCCGGAAAT GCCAGCAGAC 
GACTTGTTGT CCGCCTTCAA AGTGGCCAGC ATAGCTGCTT TCGAAGAGGA ACCGAGCGAT 
TCGGTTAGCA AGCAAGACCA AAATGCCGCC GGCGAGGAGG ATGACAGTAA GGACTGGGAC 
GACATCATTC CGGAAGGCTT CCGTAAAGCA ATCGACGATC AGGAGCGAGC AAAAGAGATG 
GAAGATCTCT ACTTGCCACC CCGGAGGAAA ACTGCCGCTA ACCAAAACGA AGGGAAGCGT 
GGAGCTGGCA AGGGGGGCAA GGGGAAACAG CAGGCAGACG ACTCCGGTGG CGACTCGGAT 
TACGAGCTGG GCTCCGACGG CAGTGGTGAC GATGGACGAC CTCGCAAGCG CGGGCGGCCG 
ACCATGAAGG AGAAGATCAC CGGATTCACT GATGCGGAGT TGCGTCGCTT CATTCGCAGT 
TACAAAAAGT TTCCCGCTCC TCTCCACCGC ATGGAGGCCA TCGCATGTGA TGCTGAGCTG 
CAGGAAAAGC CACTGGCGGA ACTGAAGCGC CTCGGAGAGA TGCTGCATGA CCGCTGCGTT 
CAGTTCCTGC ACGAGCACAA GGAGGAAGAG AGTAAGACTG CGGCGACGGA TGAGACGCCG 
GGCGCCAAGC AGCGCCGCGC ACGCGCCACC TTCTCCGTTA AGCTGGGTGG CGTCTCCTTT 
AATGCCAAAA AGCTGCTGGC CTGCGAACAG GAGCTTCAGC CGCTCAACGA GATCATGCCC 
AGCATGCCCG AAGAGCGCCA GCAATGGAGC TTCAATATCA AGACACGCGC CCCGGTCTTC 
GACGTGGATT GGGGCATCGA GGAGGACACG AAGCTACTGT GCGGCATTTA CCAGTACGGC 
ATTGGATCCT GGGAGCAGAT GAAGCTAGAC CCCACGCTCA AACTGACCGA CAAGATTTTG 
CTTAACGATA CGCGCAAGCC CCAGGCCAAG CAGCTGCAGA CGCGTGCCGA GTACCTGCTC 
AAGATCATCA AGAAGAACGT GGAGCTGACC AAGGGAGGAC AACGCCGACA ACGGCGCCCC 
AGAGCATCGC GAGCTAACGA TGCCAAGGCG GCAAGCCAGT CTGCTAGTTC GACCATCGAT 
GCTAAGCCGC ACGATGGCGA GGACGCTGCT GGCGACGCAC GCACCGTCGC CGAAAGCAGT 
AACAGTCAAG TAGATCCATC TACCGCGTCG CCGCACAATG CTCCAGCTAC GGAACAGCAC 
GGTGACCCTG CGAAGAAAGC CAAAAAATCC AAGGCTCGTT CGAAGAAAAC CAGCGCCTCG 
GACAACAACG GCAACAAGCC GATGCACTTT ACGGCGAACA ATGAACCTAG GGCTTTGGAG 
GTCCTGGGTG ACCTAGATCC CAGCATTTTT AACGAGTGCA AAGAAAAAAT GCGGCCGGTG 
AAGAAGGCCC TCAAGGCGCT GGACCAGCCG GATGTCAGTC TTTCCGACCA GGATCAGCTG 
CAGCACACGA GGGATTGCCT GCTTCAAATT GGCAAGCAGA TCGATGTTTG CCTCAATCCC 
TACGCCGAGA CAGAGAAAAA GGAATGGCGC AGTAACCTTT GGTACTTTGT GTCGAAATTC 
ACTGAGCTGG ATGCCAAGCG CCTATTCAAG ATTTACAAAC ACGCACTCAA GCAGAAAGCG 
GGCGGCGATG GCGAGGCGAA GGGAAAAGAC AAGGGCTCGT CAGGAAGTCC GGCCAAGTCT 
AAACCCAACG GTGTGACTAC TGAAGAAAAA GAAAAGGAGC GCGACAGGAG TGGGGGCAAA 
AAGAAGAAAA AGGACAAGGA CAAGGAACGC AGTGGACAGG CGCGGTATCC GGAAACGGGT 
ATACCCACCT CCGGTCGTTA CGCTGACCCT CCCCTAAAGC GTAAGCGGGA TGAGAACGAC 
GCTGATGCAA GCAGCGGACT AGCAGGTGCC CCCGGCGGAG GCATCGGCGA CAACCTCAAG 
TCCATGTCCT TCAAACGGCT GAACATGGAC CGTCACGAGG ACCGTAAGAA GCACCATCGC 
GGTCCCGACT ACTACGGTGG CAGTGGCCCA CCCATGGGAA GTGGTAGCTA CGAAGGCGGC 
AGCAATTCCC GGCGCCAGGG CCCCACCTCC CCATCTACAC CCCGTACTGG CCGGGGCGGC 
TATGATCCAC CGCCCGCGCC ATCCGGCTAC ACGCCGGAAA TGGAGCGATG GCAATCCCGC 
GATAGCCAGG ATTACAAACG GGATCGCTAC GATGGATATG GGCGCAGTGG GGGAGGTCAA 
GGCAGCTATC ATCGCGAACG CGACCGTCGT CCCGAGAAGC GCAGATATCC GTCTGGCTTG 
CCACCGCATC CTTACTCAAG CCACTACCTG CCGCCCAATT ACTATGGATT GCCTAATGGA 
GCCGTTCCGG GCCTACCGCC ACCGTCGTCT GTCTACCGCA GTGACCCACG CGGATATCCG 
GTCATGCCGC GAGACTATCC TGCCGACTAC AGACGCAGCG ACTACGAGCG GCGCACGCAG 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259975.1
CDS	204..5849
Translation	MSQALNESANSIGSDEQDDTREEANGTDHSGSGSGSGSSGSDSDSDSSSGNSSDGRSSPEPEDKSLSVAGFPPTAAAAQADSKTNGFTDDQEDSSSDGSSGSDSDSDAEGPSDQRNQSINNANTSSSLPKPEQNEEEDNETEAGQQQPASDASADESSDSSANVSPTSSSSSSEEEEEDYRPKRTRQARKPPTAAEKSKKAPAPKNKKKTWDSDESDESEDSDDEVSTAQKRKPAATTSRSKLAQQQQRRRVKPFSSEDSDDDDASKRCATRRKGAAVSYKEASEDEATDSEDLLEFEYDESQAATTAATAEEEEKCETIERILAQRAGKRGCTGNQTTIYAIEENGFDPHAGFDEKQTPDAETEAQFLIKWKGWSYIHNTWESEATLRDMKAKGMKKLDNFIKKEKEQAYWRRYAGPEDIDYFECQLELQHELLKSYNNVDRIIAKGSKPDDGTEEYLCKWQSLPYAESTWEDAALVLRKWQRCAEQFNDRESSKCTPSRHCRVIKYRPKFSRIKNQPEFLSSGLTLRDYQMDGLNWLLHSWCKENSVILADEMGLGKTIQTICFLYSLFKIHHLYGPFLCVVPLSTMTAWQREFDLWAPDMNVVTYLGDIKSRELIQQYEWQFESSKRLKFNCILTTYEIVLKDKQFLGTLQWAALLVDEAHRLKNDDSLLYKSLKEFDTNHRLLITGTPLQNSLKELWALLHFIMPDKFDTWENFEVQHGNAEDKGYTRLHQQLEPYILRRVKKDVEKSLPAKVEQILRVEMTSLQKQYYKWILTKNFDALRKGKRGSTSTFLNIVIELKKCCNHAALIRPSEFELMGLQQDEALQTLLKGSGKLVLLDKLLCRLKETGHRVLIFSQMVRMLDVLADYLQKRHFPFQRLDGSIKGEMRRQALDHFNAEGSQDFCFLLSTRAGGLGINLATADTVIIFDSDWNPQNDLQAQARAHRIGQKNQVNIYRLVTARSVEEQIVERAKQKMVLDHLVIQRMDTTGRTVLDKSGNGHSSNSNPFNKDDLSAILKFGAEELFKDEQEHDDDLVCDIDEILRRAETRNEDPEMPADDLLSAFKVASIAAFEEEPSDSVSKQDQNAAGEEDDSKDWDDIIPEGFRKAIDDQERAKEMEDLYLPPRRKTAANQNEGKRGAGKGGKGKQQADDSGGDSDYELGSDGSGDDGRPRKRGRPTMKEKITGFTDAELRRFIRSYKKFPAPLHRMEAIACDAELQEKPLAELKRLGEMLHDRCVQFLHEHKEEESKTAATDETPGAKQRRARATFSVKLGGVSFNAKKLLACEQELQPLNEIMPSMPEERQQWSFNIKTRAPVFDVDWGIEEDTKLLCGIYQYGIGSWEQMKLDPTLKLTDKILLNDTRKPQAKQLQTRAEYLLKIIKKNVELTKGGQRRQRRPRASRANDAKAASQSASSTIDAKPHDGEDAAGDARTVAESSNSQVDPSTASPHNAPATEQHGDPAKKAKKSKARSKKTSASDNNGNKPMHFTANNEPRALEVLGDLDPSIFNECKEKMRPVKKALKALDQPDVSLSDQDQLQHTRDCLLQIGKQIDVCLNPYAETEKKEWRSNLWYFVSKFTELDAKRLFKIYKHALKQKAGGDGEAKGKDKGSSGSPAKSKPNGVTTEEKEKERDRSGGKKKKKDKDKERSGQARYPETGIPTSGRYADPPLKRKRDENDADASSGLAGAPGGGIGDNLKSMSFKRLNMDRHEDRKKHHRGPDYYGGSGPPMGSGSYEGGSNSRRQGPTSPSTPRTGRGGYDPPPAPSGYTPEMERWQSRDSQDYKRDRYDGYGRSGGGQGSYHRERDRRPEKRRYPSGLPPHPYSSHYLPPNYYGLPNGAVPGLPPPSSVYRSDPRGYPVMPRDYPADYRRSDYERRTQT

Target ORF information:

RefSeq Version	NM_001273046.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Chromodomain-helicase-DNA-binding protein 1 (Chd1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273046.1

ORF Insert Sequence:

ATGAGCCAGG CACTCAATGA ATCGGCGAAT AGTATTGGCT CAGACGAACA AGATGACACT 
CGGGAAGAGG CCAATGGCAC CGACCATAGT GGCAGTGGCA GCGGTAGTGG CTCCTCCGGC 
TCAGACTCAG ATTCAGACAG CTCCTCGGGC AACTCTAGCG ATGGACGGAG CTCCCCAGAA 
CCCGAGGACA AATCGTTATC GGTGGCAGGG TTTCCACCGA CAGCCGCTGC TGCCCAGGCG 
GACAGTAAGA CGAACGGGTT CACGGATGAC CAGGAGGACA GCTCCAGTGA CGGGTCTAGT 
GGCAGTGATT CCGATTCAGA CGCCGAGGGC CCATCGGATC AAAGGAACCA GAGCATCAAC 
AATGCCAACA CAAGCAGCAG TTTGCCTAAG CCGGAGCAGA ATGAGGAGGA GGACAATGAG 
ACTGAGGCTG GTCAGCAGCA GCCAGCCAGC GATGCCTCTG CCGATGAATC AAGTGATAGT 
TCTGCCAATG TGTCACCCAC ATCCTCCAGC AGCAGCAGTG AGGAGGAAGA GGAGGACTAT 
AGACCCAAGC GGACGCGCCA GGCGCGCAAA CCACCGACTG CTGCTGAGAA GTCGAAAAAA 
GCTCCAGCAC CCAAGAACAA GAAGAAAACC TGGGACTCGG ATGAAAGCGA TGAAAGCGAG 
GACAGTGATG ACGAGGTGTC CACCGCCCAA AAGCGCAAAC CGGCAGCAAC TACCTCTCGC 
AGCAAACTCG CACAGCAACA GCAGCGGCGC AGGGTGAAAC CCTTTAGTTC CGAGGACAGT 
GATGATGACG ACGCTAGCAA ACGCTGCGCT ACTCGTCGCA AGGGTGCTGC CGTTAGCTAC 
AAGGAGGCTT CGGAGGACGA AGCCACCGAC TCCGAGGATC TGCTGGAGTT TGAGTACGAT 
GAAAGCCAGG CAGCCACCAC TGCCGCTACG GCTGAGGAGG AGGAAAAGTG CGAAACCATA 
GAGCGTATCC TGGCGCAGCG TGCTGGGAAG AGGGGATGCA CGGGCAATCA GACAACGATC 
TATGCAATAG AGGAAAACGG CTTTGATCCA CATGCTGGAT TTGATGAAAA GCAAACTCCA 
GACGCGGAGA CCGAGGCACA GTTCCTGATC AAGTGGAAGG GCTGGTCGTA CATCCACAAC 
ACCTGGGAAT CTGAGGCTAC CCTGCGTGAT ATGAAGGCCA AGGGAATGAA GAAGCTGGAT 
AACTTCATCA AAAAGGAGAA GGAACAGGCC TACTGGCGCC GATACGCTGG CCCCGAGGAC 
ATCGACTACT TCGAGTGTCA GCTGGAACTG CAGCATGAGC TACTGAAATC GTACAACAAC 
GTGGACCGAA TCATTGCCAA GGGCTCTAAG CCAGACGACG GCACCGAGGA GTACCTGTGC 
AAATGGCAGT CGCTACCGTA CGCCGAGTCC ACATGGGAGG ATGCCGCCCT GGTTCTTCGC 
AAGTGGCAAC GCTGCGCAGA GCAATTCAAT GACCGCGAGT CCTCCAAATG CACTCCCTCC 
CGCCACTGCA GAGTGATCAA GTATCGCCCC AAGTTCTCTC GAATAAAGAA TCAGCCGGAA 
TTTCTTTCGT CGGGCCTAAC TCTAAGGGAT TACCAAATGG ACGGCCTGAA TTGGCTGCTG 
CACTCATGGT GCAAGGAGAA CTCGGTGATC CTGGCCGACG AGATGGGCCT CGGCAAGACC 
ATCCAAACTA TTTGCTTCCT GTACTCGCTG TTCAAGATCC ATCACCTGTA CGGCCCGTTC 
CTGTGCGTGG TGCCACTTAG CACAATGACG GCCTGGCAGC GGGAGTTCGA CCTGTGGGCG 
CCGGACATGA ATGTAGTCAC GTACCTCGGT GACATTAAAT CCCGCGAGCT CATTCAACAG 
TACGAATGGC AGTTTGAGAG CTCCAAGCGG CTTAAATTCA ATTGCATTCT CACAACGTAC 
GAGATTGTGC TCAAGGATAA ACAATTTCTG GGCACGCTTC AGTGGGCTGC GCTCCTGGTG 
GACGAAGCGC ATCGTCTTAA GAACGACGAC TCACTGCTCT ACAAGTCGCT GAAGGAGTTT 
GACACTAACC ATCGACTGCT CATAACAGGT ACCCCGCTGC AGAATTCGCT GAAGGAGCTC 
TGGGCTTTGC TGCACTTCAT CATGCCGGAT AAGTTTGACA CGTGGGAGAA TTTCGAGGTG 
CAGCATGGCA ATGCGGAGGA CAAGGGCTAC ACTCGGTTGC ATCAACAGCT GGAGCCGTAT 
ATTCTGCGGA GAGTGAAGAA GGATGTGGAG AAATCGCTGC CGGCTAAAGT GGAGCAGATC 
CTGCGGGTCG AGATGACCTC TCTGCAGAAA CAGTACTACA AGTGGATCCT AACCAAAAAC 
TTTGATGCAC TGCGCAAGGG AAAGCGGGGC AGTACCTCCA CTTTCCTCAA CATAGTCATC 
GAGCTAAAGA AGTGCTGCAA CCACGCGGCT CTCATCCGAC CATCCGAGTT TGAGCTTATG 
GGTCTGCAGC AGGATGAGGC ATTGCAAACT CTTCTTAAGG GATCCGGAAA GCTTGTGCTG 
CTCGATAAGC TACTCTGCCG ACTGAAAGAG ACGGGCCACC GCGTGCTGAT TTTTTCCCAA 
ATGGTGCGCA TGCTGGACGT TCTGGCTGAC TATCTGCAGA AGCGTCACTT CCCTTTCCAG 
CGCCTCGACG GCAGCATAAA GGGAGAGATG AGGCGCCAGG CTCTGGATCA CTTCAATGCA 
GAGGGTAGCC AGGACTTTTG CTTCCTGCTT TCGACAAGGG CTGGTGGATT GGGCATTAAC 
TTGGCCACAG CCGATACTGT TATCATCTTT GACTCCGACT GGAACCCCCA AAACGATTTA 
CAGGCGCAGG CGAGAGCGCA TCGAATCGGT CAGAAAAACC AGGTTAACAT TTATCGCCTG 
GTCACCGCCC GGTCGGTGGA GGAACAGATC GTGGAGCGAG CCAAACAGAA AATGGTACTG 
GACCACCTGG TCATTCAGCG GATGGACACT ACAGGACGCA CTGTTCTGGA TAAGAGCGGC 
AACGGGCACT CGTCTAACTC GAATCCGTTC AACAAGGACG ATCTTTCTGC TATCCTGAAG 
TTTGGTGCAG AGGAGCTGTT CAAGGACGAA CAGGAGCATG ACGACGACTT GGTTTGTGAC 
ATTGATGAAA TTCTGCGCAG GGCGGAGACC CGCAACGAAG ATCCGGAAAT GCCAGCAGAC 
GACTTGTTGT CCGCCTTCAA AGTGGCCAGC ATAGCTGCTT TCGAAGAGGA ACCGAGCGAT 
TCGGTTAGCA AGCAAGACCA AAATGCCGCC GGCGAGGAGG ATGACAGTAA GGACTGGGAC 
GACATCATTC CGGAAGGCTT CCGTAAAGCA ATCGACGATC AGGAGCGAGC AAAAGAGATG 
GAAGATCTCT ACTTGCCACC CCGGAGGAAA ACTGCCGCTA ACCAAAACGA AGGGAAGCGT 
GGAGCTGGCA AGGGGGGCAA GGGGAAACAG CAGGCAGACG ACTCCGGTGG CGACTCGGAT 
TACGAGCTGG GCTCCGACGG CAGTGGTGAC GATGGACGAC CTCGCAAGCG CGGGCGGCCG 
ACCATGAAGG AGAAGATCAC CGGATTCACT GATGCGGAGT TGCGTCGCTT CATTCGCAGT 
TACAAAAAGT TTCCCGCTCC TCTCCACCGC ATGGAGGCCA TCGCATGTGA TGCTGAGCTG 
CAGGAAAAGC CACTGGCGGA ACTGAAGCGC CTCGGAGAGA TGCTGCATGA CCGCTGCGTT 
CAGTTCCTGC ACGAGCACAA GGAGGAAGAG AGTAAGACTG CGGCGACGGA TGAGACGCCG 
GGCGCCAAGC AGCGCCGCGC ACGCGCCACC TTCTCCGTTA AGCTGGGTGG CGTCTCCTTT 
AATGCCAAAA AGCTGCTGGC CTGCGAACAG GAGCTTCAGC CGCTCAACGA GATCATGCCC 
AGCATGCCCG AAGAGCGCCA GCAATGGAGC TTCAATATCA AGACACGCGC CCCGGTCTTC 
GACGTGGATT GGGGCATCGA GGAGGACACG AAGCTACTGT GCGGCATTTA CCAGTACGGC 
ATTGGATCCT GGGAGCAGAT GAAGCTAGAC CCCACGCTCA AACTGACCGA CAAGATTTTG 
CTTAACGATA CGCGCAAGCC CCAGGCCAAG CAGCTGCAGA CGCGTGCCGA GTACCTGCTC 
AAGATCATCA AGAAGAACGT GGAGCTGACC AAGGGAGGAC AACGCCGACA ACGGCGCCCC 
AGAGCATCGC GAGCTAACGA TGCCAAGGCG GCAAGCCAGT CTGCTAGTTC GACCATCGAT 
GCTAAGCCGC ACGATGGCGA GGACGCTGCT GGCGACGCAC GCACCGTCGC CGAAAGCAGT 
AACAGTCAAG TAGATCCATC TACCGCGTCG CCGCACAATG CTCCAGCTAC GGAACAGCAC 
GGTGACCCTG CGAAGAAAGC CAAAAAATCC AAGGCTCGTT CGAAGAAAAC CAGCGCCTCG 
GACAACAACG GCAACAAGCC GATGCACTTT ACGGCGAACA ATGAACCTAG GGCTTTGGAG 
GTCCTGGGTG ACCTAGATCC CAGCATTTTT AACGAGTGCA AAGAAAAAAT GCGGCCGGTG 
AAGAAGGCCC TCAAGGCGCT GGACCAGCCG GATGTCAGTC TTTCCGACCA GGATCAGCTG 
CAGCACACGA GGGATTGCCT GCTTCAAATT GGCAAGCAGA TCGATGTTTG CCTCAATCCC 
TACGCCGAGA CAGAGAAAAA GGAATGGCGC AGTAACCTTT GGTACTTTGT GTCGAAATTC 
ACTGAGCTGG ATGCCAAGCG CCTATTCAAG ATTTACAAAC ACGCACTCAA GCAGAAAGCG 
GGCGGCGATG GCGAGGCGAA GGGAAAAGAC AAGGGCTCGT CAGGAAGTCC GGCCAAGTCT 
AAACCCAACG GTGTGACTAC TGAAGAAAAA GAAAAGGAGC GCGACAGGAG TGGGGGCAAA 
AAGAAGAAAA AGGACAAGGA CAAGGAACGC AGTGGACAGG CGCGGTATCC GGAAACGGGT 
ATACCCACCT CCGGTCGTTA CGCTGACCCT CCCCTAAAGC GTAAGCGGGA TGAGAACGAC 
GCTGATGCAA GCAGCGGACT AGCAGGTGCC CCCGGCGGAG GCATCGGCGA CAACCTCAAG 
TCCATGTCCT TCAAACGGCT GAACATGGAC CGTCACGAGG ACCGTAAGAA GCACCATCGC 
GGTCCCGACT ACTACGGTGG CAGTGGCCCA CCCATGGGAA GTGGTAGCTA CGAAGGCGGC 
AGCAATTCCC GGCGCCAGGG CCCCACCTCC CCATCTACAC CCCGTACTGG CCGGGGCGGC 
TATGATCCAC CGCCCGCGCC ATCCGGCTAC ACGCCGGAAA TGGAGCGATG GCAATCCCGC 
GATAGCCAGG ATTACAAACG GGATCGCTAC GATGGATATG GGCGCAGTGG GGGAGGTCAA 
GGCAGCTATC ATCGCGAACG CGACCGTCGT CCCGAGAAGC GCAGATATCC GTCTGGCTTG 
CCACCGCATC CTTACTCAAG CCACTACCTG CCGCCCAATT ACTATGGATT GCCTAATGGA 
GCCGTTCCGG GCCTACCGCC ACCGTCGTCT GTCTACCGCA GTGACCCACG CGGATATCCG 
GTCATGCCGC GAGACTATCC TGCCGACTAC AGACGCAGCG ACTACGAGCG GCGCACGCAG 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd1 ( NM_001258922.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245851.1 Drosophila melanogaster Chromodomain-helicase-DNA-binding protein 1 (Chd1), transcript variant B, mRNA.

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CloneID	OFa16138
Clone ID Related Accession (Same CDS sequence)	NM_001258922.1
Accession Version	NM_001258922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Chromodomain-helicase-DNA-binding protein 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTTCT TATATCATTT GAAACCAGAC AAGTCAAAAC AGTTTGATCC AGGCTGTCGT 
GCACTCAATG AATCGGCGAA TAGTATTGGC TCAGACGAAC AAGATGACAC TCGGGAAGAG 
GCCAATGGCA CCGACCATAG TGGCAGTGGC AGCGGTAGTG GCTCCTCCGG CTCAGACTCA 
GATTCAGACA GCTCCTCGGG CAACTCTAGC GATGGACGGA GCTCCCCAGA ACCCGAGGAC 
AAATCGTTAT CGGTGGCAGG GTTTCCACCG ACAGCCGCTG CTGCCCAGGC GGACAGTAAG 
ACGAACGGGT TCACGGATGA CCAGGAGGAC AGCTCCAGTG ACGGGTCTAG TGGCAGTGAT 
TCCGATTCAG ACGCCGAGGG CCCATCGGAT CAAAGGAACC AGAGCATCAA CAATGCCAAC 
ACAAGCAGCA GTTTGCCTAA GCCGGAGCAG AATGAGGAGG AGGACAATGA GACTGAGGCT 
GGTCAGCAGC AGCCAGCCAG CGATGCCTCT GCCGATGAAT CAAGTGATAG TTCTGCCAAT 
GTGTCACCCA CATCCTCCAG CAGCAGCAGT GAGGAGGAAG AGGAGGACTA TAGACCCAAG 
CGGACGCGCC AGGCGCGCAA ACCACCGACT GCTGCTGAGA AGTCGAAAAA AGCTCCAGCA 
CCCAAGAACA AGAAGAAAAC CTGGGACTCG GATGAAAGCG ATGAAAGCGA GGACAGTGAT 
GACGAGGTGT CCACCGCCCA AAAGCGCAAA CCGGCAGCAA CTACCTCTCG CAGCAAACTC 
GCACAGCAAC AGCAGCGGCG CAGGGTGAAA CCCTTTAGTT CCGAGGACAG TGATGATGAC 
GACGCTAGCA AACGCTGCGC TACTCGTCGC AAGGGTGCTG CCGTTAGCTA CAAGGAGGCT 
TCGGAGGACG AAGCCACCGA CTCCGAGGAT CTGCTGGAGT TTGAGTACGA TGAAAGCCAG 
GCAGCCACCA CTGCCGCTAC GGCTGAGGAG GAGGAAAAGT GCGAAACCAT AGAGCGTATC 
CTGGCGCAGC GTGCTGGGAA GAGGGGATGC ACGGGCAATC AGACAACGAT CTATGCAATA 
GAGGAAAACG GCTTTGATCC ACATGCTGGA TTTGATGAAA AGCAAACTCC AGACGCGGAG 
ACCGAGGCAC AGTTCCTGAT CAAGTGGAAG GGCTGGTCGT ACATCCACAA CACCTGGGAA 
TCTGAGGCTA CCCTGCGTGA TATGAAGGCC AAGGGAATGA AGAAGCTGGA TAACTTCATC 
AAAAAGGAGA AGGAACAGGC CTACTGGCGC CGATACGCTG GCCCCGAGGA CATCGACTAC 
TTCGAGTGTC AGCTGGAACT GCAGCATGAG CTACTGAAAT CGTACAACAA CGTGGACCGA 
ATCATTGCCA AGGGCTCTAA GCCAGACGAC GGCACCGAGG AGTACCTGTG CAAATGGCAG 
TCGCTACCGT ACGCCGAGTC CACATGGGAG GATGCCGCCC TGGTTCTTCG CAAGTGGCAA 
CGCTGCGCAG AGCAATTCAA TGACCGCGAG TCCTCCAAAT GCACTCCCTC CCGCCACTGC 
AGAGTGATCA AGTATCGCCC CAAGTTCTCT CGAATAAAGA ATCAGCCGGA ATTTCTTTCG 
TCGGGCCTAA CTCTAAGGGA TTACCAAATG GACGGCCTGA ATTGGCTGCT GCACTCATGG 
TGCAAGGAGA ACTCGGTGAT CCTGGCCGAC GAGATGGGCC TCGGCAAGAC CATCCAAACT 
ATTTGCTTCC TGTACTCGCT GTTCAAGATC CATCACCTGT ACGGCCCGTT CCTGTGCGTG 
GTGCCACTTA GCACAATGAC GGCCTGGCAG CGGGAGTTCG ACCTGTGGGC GCCGGACATG 
AATGTAGTCA CGTACCTCGG TGACATTAAA TCCCGCGAGC TCATTCAACA GTACGAATGG 
CAGTTTGAGA GCTCCAAGCG GCTTAAATTC AATTGCATTC TCACAACGTA CGAGATTGTG 
CTCAAGGATA AACAATTTCT GGGCACGCTT CAGTGGGCTG CGCTCCTGGT GGACGAAGCG 
CATCGTCTTA AGAACGACGA CTCACTGCTC TACAAGTCGC TGAAGGAGTT TGACACTAAC 
CATCGACTGC TCATAACAGG TACCCCGCTG CAGAATTCGC TGAAGGAGCT CTGGGCTTTG 
CTGCACTTCA TCATGCCGGA TAAGTTTGAC ACGTGGGAGA ATTTCGAGGT GCAGCATGGC 
AATGCGGAGG ACAAGGGCTA CACTCGGTTG CATCAACAGC TGGAGCCGTA TATTCTGCGG 
AGAGTGAAGA AGGATGTGGA GAAATCGCTG CCGGCTAAAG TGGAGCAGAT CCTGCGGGTC 
GAGATGACCT CTCTGCAGAA ACAGTACTAC AAGTGGATCC TAACCAAAAA CTTTGATGCA 
CTGCGCAAGG GAAAGCGGGG CAGTACCTCC ACTTTCCTCA ACATAGTCAT CGAGCTAAAG 
AAGTGCTGCA ACCACGCGGC TCTCATCCGA CCATCCGAGT TTGAGCTTAT GGGTCTGCAG 
CAGGATGAGG CATTGCAAAC TCTTCTTAAG GGATCCGGAA AGCTTGTGCT GCTCGATAAG 
CTACTCTGCC GACTGAAAGA GACGGGCCAC CGCGTGCTGA TTTTTTCCCA AATGGTGCGC 
ATGCTGGACG TTCTGGCTGA CTATCTGCAG AAGCGTCACT TCCCTTTCCA GCGCCTCGAC 
GGCAGCATAA AGGGAGAGAT GAGGCGCCAG GCTCTGGATC ACTTCAATGC AGAGGGTAGC 
CAGGACTTTT GCTTCCTGCT TTCGACAAGG GCTGGTGGAT TGGGCATTAA CTTGGCCACA 
GCCGATACTG TTATCATCTT TGACTCCGAC TGGAACCCCC AAAACGATTT ACAGGCGCAG 
GCGAGAGCGC ATCGAATCGG TCAGAAAAAC CAGGTTAACA TTTATCGCCT GGTCACCGCC 
CGGTCGGTGG AGGAACAGAT CGTGGAGCGA GCCAAACAGA AAATGGTACT GGACCACCTG 
GTCATTCAGC GGATGGACAC TACAGGACGC ACTGTTCTGG ATAAGAGCGG CAACGGGCAC 
TCGTCTAACT CGAATCCGTT CAACAAGGAC GATCTTTCTG CTATCCTGAA GTTTGGTGCA 
GAGGAGCTGT TCAAGGACGA ACAGGAGCAT GACGACGACT TGGTTTGTGA CATTGATGAA 
ATTCTGCGCA GGGCGGAGAC CCGCAACGAA GATCCGGAAA TGCCAGCAGA CGACTTGTTG 
TCCGCCTTCA AAGTGGCCAG CATAGCTGCT TTCGAAGAGG AACCGAGCGA TTCGGTTAGC 
AAGCAAGACC AAAATGCCGC CGGCGAGGAG GATGACAGTA AGGACTGGGA CGACATCATT 
CCGGAAGGCT TCCGTAAAGC AATCGACGAT CAGGAGCGAG CAAAAGAGAT GGAAGATCTC 
TACTTGCCAC CCCGGAGGAA AACTGCCGCT AACCAAAACG AAGGGAAGCG TGGAGCTGGC 
AAGGGGGGCA AGGGGAAACA GCAGGCAGAC GACTCCGGTG GCGACTCGGA TTACGAGCTG 
GGCTCCGACG GCAGTGGTGA CGATGGACGA CCTCGCAAGC GCGGGCGGCC GACCATGAAG 
GAGAAGATCA CCGGATTCAC TGATGCGGAG TTGCGTCGCT TCATTCGCAG TTACAAAAAG 
TTTCCCGCTC CTCTCCACCG CATGGAGGCC ATCGCATGTG ATGCTGAGCT GCAGGAAAAG 
CCACTGGCGG AACTGAAGCG CCTCGGAGAG ATGCTGCATG ACCGCTGCGT TCAGTTCCTG 
CACGAGCACA AGGAGGAAGA GAGTAAGACT GCGGCGACGG ATGAGACGCC GGGCGCCAAG 
CAGCGCCGCG CACGCGCCAC CTTCTCCGTT AAGCTGGGTG GCGTCTCCTT TAATGCCAAA 
AAGCTGCTGG CCTGCGAACA GGAGCTTCAG CCGCTCAACG AGATCATGCC CAGCATGCCC 
GAAGAGCGCC AGCAATGGAG CTTCAATATC AAGACACGCG CCCCGGTCTT CGACGTGGAT 
TGGGGCATCG AGGAGGACAC GAAGCTACTG TGCGGCATTT ACCAGTACGG CATTGGATCC 
TGGGAGCAGA TGAAGCTAGA CCCCACGCTC AAACTGACCG ACAAGATTTT GCTTAACGAT 
ACGCGCAAGC CCCAGGCCAA GCAGCTGCAG ACGCGTGCCG AGTACCTGCT CAAGATCATC 
AAGAAGAACG TGGAGCTGAC CAAGGGAGGA CAACGCCGAC AACGGCGCCC CAGAGCATCG 
CGAGCTAACG ATGCCAAGGC GGCAAGCCAG TCTGCTAGTT CGACCATCGA TGCTAAGCCG 
CACGATGGCG AGGACGCTGC TGGCGACGCA CGCACCGTCG CCGAAAGCAG TAACAGTCAA 
GTAGATCCAT CTACCGCGTC GCCGCACAAT GCTCCAGCTA CGGAACAGCA CGGTGACCCT 
GCGAAGAAAG CCAAAAAATC CAAGGCTCGT TCGAAGAAAA CCAGCGCCTC GGACAACAAC 
GGCAACAAGC CGATGCACTT TACGGCGAAC AATGAACCTA GGGCTTTGGA GGTCCTGGGT 
GACCTAGATC CCAGCATTTT TAACGAGTGC AAAGAAAAAA TGCGGCCGGT GAAGAAGGCC 
CTCAAGGCGC TGGACCAGCC GGATGTCAGT CTTTCCGACC AGGATCAGCT GCAGCACACG 
AGGGATTGCC TGCTTCAAAT TGGCAAGCAG ATCGATGTTT GCCTCAATCC CTACGCCGAG 
ACAGAGAAAA AGGAATGGCG CAGTAACCTT TGGTACTTTG TGTCGAAATT CACTGAGCTG 
GATGCCAAGC GCCTATTCAA GATTTACAAA CACGCACTCA AGCAGAAAGC GGGCGGCGAT 
GGCGAGGCGA AGGGAAAAGA CAAGGGCTCG TCAGGAAGTC CGGCCAAGTC TAAACCCAAC 
GGTGTGACTA CTGAAGAAAA AGAAAAGGAG CGCGACAGGA GTGGGGGCAA AAAGAAGAAA 
AAGGACAAGG ACAAGGAACG CAGTGGACAG GCGCGGTATC CGGAAACGGG TATACCCACC 
TCCGGTCGTT ACGCTGACCC TCCCCTAAAG CGTAAGCGGG ATGAGAACGA CGCTGATGCA 
AGCAGCGGAC TAGCAGGTGC CCCCGGCGGA GGCATCGGCG ACAACCTCAA GTCCATGTCC 
TTCAAACGGC TGAACATGGA CCGTCACGAG GACCGTAAGA AGCACCATCG CGGTCCCGAC 
TACTACGGTG GCAGTGGCCC ACCCATGGGA AGTGGTAGCT ACGAAGGCGG CAGCAATTCC 
CGGCGCCAGG GCCCCACCTC CCCATCTACA CCCCGTACTG GCCGGGGCGG CTATGATCCA 
CCGCCCGCGC CATCCGGCTA CACGCCGGAA ATGGAGCGAT GGCAATCCCG CGATAGATAC 
AGCCAGGATT ACAAACGGGA TCGCTACGAT GGATATGGGC GCAGTGGGGG AGGTCAAGGC 
AGCTATCATC GCGAACGCGA CCGTCGTCCC GAGAAGCGCA GATATCCGTC TGGCTTGCCA 
CCGCATCCTT ACTCAAGCCA CTACCTGCCG CCCAATTACT ATGGATTGCC TAATGGAGCC 
GTTCCGGGCC TACCGCCACC GTCGTCTGTC TACCGCAGTG ACCCACGCGG ATATCCGGTC 
ATGCCGCGAG ACTATCCTGC CGACTACAGA CGCAGCGACT ACGAGCGGCG CACGCAGACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245851.1
CDS	37..5739
Translation	MNFLYHLKPDKSKQFDPGCRALNESANSIGSDEQDDTREEANGTDHSGSGSGSGSSGSDSDSDSSSGNSSDGRSSPEPEDKSLSVAGFPPTAAAAQADSKTNGFTDDQEDSSSDGSSGSDSDSDAEGPSDQRNQSINNANTSSSLPKPEQNEEEDNETEAGQQQPASDASADESSDSSANVSPTSSSSSSEEEEEDYRPKRTRQARKPPTAAEKSKKAPAPKNKKKTWDSDESDESEDSDDEVSTAQKRKPAATTSRSKLAQQQQRRRVKPFSSEDSDDDDASKRCATRRKGAAVSYKEASEDEATDSEDLLEFEYDESQAATTAATAEEEEKCETIERILAQRAGKRGCTGNQTTIYAIEENGFDPHAGFDEKQTPDAETEAQFLIKWKGWSYIHNTWESEATLRDMKAKGMKKLDNFIKKEKEQAYWRRYAGPEDIDYFECQLELQHELLKSYNNVDRIIAKGSKPDDGTEEYLCKWQSLPYAESTWEDAALVLRKWQRCAEQFNDRESSKCTPSRHCRVIKYRPKFSRIKNQPEFLSSGLTLRDYQMDGLNWLLHSWCKENSVILADEMGLGKTIQTICFLYSLFKIHHLYGPFLCVVPLSTMTAWQREFDLWAPDMNVVTYLGDIKSRELIQQYEWQFESSKRLKFNCILTTYEIVLKDKQFLGTLQWAALLVDEAHRLKNDDSLLYKSLKEFDTNHRLLITGTPLQNSLKELWALLHFIMPDKFDTWENFEVQHGNAEDKGYTRLHQQLEPYILRRVKKDVEKSLPAKVEQILRVEMTSLQKQYYKWILTKNFDALRKGKRGSTSTFLNIVIELKKCCNHAALIRPSEFELMGLQQDEALQTLLKGSGKLVLLDKLLCRLKETGHRVLIFSQMVRMLDVLADYLQKRHFPFQRLDGSIKGEMRRQALDHFNAEGSQDFCFLLSTRAGGLGINLATADTVIIFDSDWNPQNDLQAQARAHRIGQKNQVNIYRLVTARSVEEQIVERAKQKMVLDHLVIQRMDTTGRTVLDKSGNGHSSNSNPFNKDDLSAILKFGAEELFKDEQEHDDDLVCDIDEILRRAETRNEDPEMPADDLLSAFKVASIAAFEEEPSDSVSKQDQNAAGEEDDSKDWDDIIPEGFRKAIDDQERAKEMEDLYLPPRRKTAANQNEGKRGAGKGGKGKQQADDSGGDSDYELGSDGSGDDGRPRKRGRPTMKEKITGFTDAELRRFIRSYKKFPAPLHRMEAIACDAELQEKPLAELKRLGEMLHDRCVQFLHEHKEEESKTAATDETPGAKQRRARATFSVKLGGVSFNAKKLLACEQELQPLNEIMPSMPEERQQWSFNIKTRAPVFDVDWGIEEDTKLLCGIYQYGIGSWEQMKLDPTLKLTDKILLNDTRKPQAKQLQTRAEYLLKIIKKNVELTKGGQRRQRRPRASRANDAKAASQSASSTIDAKPHDGEDAAGDARTVAESSNSQVDPSTASPHNAPATEQHGDPAKKAKKSKARSKKTSASDNNGNKPMHFTANNEPRALEVLGDLDPSIFNECKEKMRPVKKALKALDQPDVSLSDQDQLQHTRDCLLQIGKQIDVCLNPYAETEKKEWRSNLWYFVSKFTELDAKRLFKIYKHALKQKAGGDGEAKGKDKGSSGSPAKSKPNGVTTEEKEKERDRSGGKKKKKDKDKERSGQARYPETGIPTSGRYADPPLKRKRDENDADASSGLAGAPGGGIGDNLKSMSFKRLNMDRHEDRKKHHRGPDYYGGSGPPMGSGSYEGGSNSRRQGPTSPSTPRTGRGGYDPPPAPSGYTPEMERWQSRDRYSQDYKRDRYDGYGRSGGGQGSYHRERDRRPEKRRYPSGLPPHPYSSHYLPPNYYGLPNGAVPGLPPPSSVYRSDPRGYPVMPRDYPADYRRSDYERRTQT

Target ORF information:

RefSeq Version	NM_001258922.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Chromodomain-helicase-DNA-binding protein 1 (Chd1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258922.1

ORF Insert Sequence:

ATGAATTTCT TATATCATTT GAAACCAGAC AAGTCAAAAC AGTTTGATCC AGGCTGTCGT 
GCACTCAATG AATCGGCGAA TAGTATTGGC TCAGACGAAC AAGATGACAC TCGGGAAGAG 
GCCAATGGCA CCGACCATAG TGGCAGTGGC AGCGGTAGTG GCTCCTCCGG CTCAGACTCA 
GATTCAGACA GCTCCTCGGG CAACTCTAGC GATGGACGGA GCTCCCCAGA ACCCGAGGAC 
AAATCGTTAT CGGTGGCAGG GTTTCCACCG ACAGCCGCTG CTGCCCAGGC GGACAGTAAG 
ACGAACGGGT TCACGGATGA CCAGGAGGAC AGCTCCAGTG ACGGGTCTAG TGGCAGTGAT 
TCCGATTCAG ACGCCGAGGG CCCATCGGAT CAAAGGAACC AGAGCATCAA CAATGCCAAC 
ACAAGCAGCA GTTTGCCTAA GCCGGAGCAG AATGAGGAGG AGGACAATGA GACTGAGGCT 
GGTCAGCAGC AGCCAGCCAG CGATGCCTCT GCCGATGAAT CAAGTGATAG TTCTGCCAAT 
GTGTCACCCA CATCCTCCAG CAGCAGCAGT GAGGAGGAAG AGGAGGACTA TAGACCCAAG 
CGGACGCGCC AGGCGCGCAA ACCACCGACT GCTGCTGAGA AGTCGAAAAA AGCTCCAGCA 
CCCAAGAACA AGAAGAAAAC CTGGGACTCG GATGAAAGCG ATGAAAGCGA GGACAGTGAT 
GACGAGGTGT CCACCGCCCA AAAGCGCAAA CCGGCAGCAA CTACCTCTCG CAGCAAACTC 
GCACAGCAAC AGCAGCGGCG CAGGGTGAAA CCCTTTAGTT CCGAGGACAG TGATGATGAC 
GACGCTAGCA AACGCTGCGC TACTCGTCGC AAGGGTGCTG CCGTTAGCTA CAAGGAGGCT 
TCGGAGGACG AAGCCACCGA CTCCGAGGAT CTGCTGGAGT TTGAGTACGA TGAAAGCCAG 
GCAGCCACCA CTGCCGCTAC GGCTGAGGAG GAGGAAAAGT GCGAAACCAT AGAGCGTATC 
CTGGCGCAGC GTGCTGGGAA GAGGGGATGC ACGGGCAATC AGACAACGAT CTATGCAATA 
GAGGAAAACG GCTTTGATCC ACATGCTGGA TTTGATGAAA AGCAAACTCC AGACGCGGAG 
ACCGAGGCAC AGTTCCTGAT CAAGTGGAAG GGCTGGTCGT ACATCCACAA CACCTGGGAA 
TCTGAGGCTA CCCTGCGTGA TATGAAGGCC AAGGGAATGA AGAAGCTGGA TAACTTCATC 
AAAAAGGAGA AGGAACAGGC CTACTGGCGC CGATACGCTG GCCCCGAGGA CATCGACTAC 
TTCGAGTGTC AGCTGGAACT GCAGCATGAG CTACTGAAAT CGTACAACAA CGTGGACCGA 
ATCATTGCCA AGGGCTCTAA GCCAGACGAC GGCACCGAGG AGTACCTGTG CAAATGGCAG 
TCGCTACCGT ACGCCGAGTC CACATGGGAG GATGCCGCCC TGGTTCTTCG CAAGTGGCAA 
CGCTGCGCAG AGCAATTCAA TGACCGCGAG TCCTCCAAAT GCACTCCCTC CCGCCACTGC 
AGAGTGATCA AGTATCGCCC CAAGTTCTCT CGAATAAAGA ATCAGCCGGA ATTTCTTTCG 
TCGGGCCTAA CTCTAAGGGA TTACCAAATG GACGGCCTGA ATTGGCTGCT GCACTCATGG 
TGCAAGGAGA ACTCGGTGAT CCTGGCCGAC GAGATGGGCC TCGGCAAGAC CATCCAAACT 
ATTTGCTTCC TGTACTCGCT GTTCAAGATC CATCACCTGT ACGGCCCGTT CCTGTGCGTG 
GTGCCACTTA GCACAATGAC GGCCTGGCAG CGGGAGTTCG ACCTGTGGGC GCCGGACATG 
AATGTAGTCA CGTACCTCGG TGACATTAAA TCCCGCGAGC TCATTCAACA GTACGAATGG 
CAGTTTGAGA GCTCCAAGCG GCTTAAATTC AATTGCATTC TCACAACGTA CGAGATTGTG 
CTCAAGGATA AACAATTTCT GGGCACGCTT CAGTGGGCTG CGCTCCTGGT GGACGAAGCG 
CATCGTCTTA AGAACGACGA CTCACTGCTC TACAAGTCGC TGAAGGAGTT TGACACTAAC 
CATCGACTGC TCATAACAGG TACCCCGCTG CAGAATTCGC TGAAGGAGCT CTGGGCTTTG 
CTGCACTTCA TCATGCCGGA TAAGTTTGAC ACGTGGGAGA ATTTCGAGGT GCAGCATGGC 
AATGCGGAGG ACAAGGGCTA CACTCGGTTG CATCAACAGC TGGAGCCGTA TATTCTGCGG 
AGAGTGAAGA AGGATGTGGA GAAATCGCTG CCGGCTAAAG TGGAGCAGAT CCTGCGGGTC 
GAGATGACCT CTCTGCAGAA ACAGTACTAC AAGTGGATCC TAACCAAAAA CTTTGATGCA 
CTGCGCAAGG GAAAGCGGGG CAGTACCTCC ACTTTCCTCA ACATAGTCAT CGAGCTAAAG 
AAGTGCTGCA ACCACGCGGC TCTCATCCGA CCATCCGAGT TTGAGCTTAT GGGTCTGCAG 
CAGGATGAGG CATTGCAAAC TCTTCTTAAG GGATCCGGAA AGCTTGTGCT GCTCGATAAG 
CTACTCTGCC GACTGAAAGA GACGGGCCAC CGCGTGCTGA TTTTTTCCCA AATGGTGCGC 
ATGCTGGACG TTCTGGCTGA CTATCTGCAG AAGCGTCACT TCCCTTTCCA GCGCCTCGAC 
GGCAGCATAA AGGGAGAGAT GAGGCGCCAG GCTCTGGATC ACTTCAATGC AGAGGGTAGC 
CAGGACTTTT GCTTCCTGCT TTCGACAAGG GCTGGTGGAT TGGGCATTAA CTTGGCCACA 
GCCGATACTG TTATCATCTT TGACTCCGAC TGGAACCCCC AAAACGATTT ACAGGCGCAG 
GCGAGAGCGC ATCGAATCGG TCAGAAAAAC CAGGTTAACA TTTATCGCCT GGTCACCGCC 
CGGTCGGTGG AGGAACAGAT CGTGGAGCGA GCCAAACAGA AAATGGTACT GGACCACCTG 
GTCATTCAGC GGATGGACAC TACAGGACGC ACTGTTCTGG ATAAGAGCGG CAACGGGCAC 
TCGTCTAACT CGAATCCGTT CAACAAGGAC GATCTTTCTG CTATCCTGAA GTTTGGTGCA 
GAGGAGCTGT TCAAGGACGA ACAGGAGCAT GACGACGACT TGGTTTGTGA CATTGATGAA 
ATTCTGCGCA GGGCGGAGAC CCGCAACGAA GATCCGGAAA TGCCAGCAGA CGACTTGTTG 
TCCGCCTTCA AAGTGGCCAG CATAGCTGCT TTCGAAGAGG AACCGAGCGA TTCGGTTAGC 
AAGCAAGACC AAAATGCCGC CGGCGAGGAG GATGACAGTA AGGACTGGGA CGACATCATT 
CCGGAAGGCT TCCGTAAAGC AATCGACGAT CAGGAGCGAG CAAAAGAGAT GGAAGATCTC 
TACTTGCCAC CCCGGAGGAA AACTGCCGCT AACCAAAACG AAGGGAAGCG TGGAGCTGGC 
AAGGGGGGCA AGGGGAAACA GCAGGCAGAC GACTCCGGTG GCGACTCGGA TTACGAGCTG 
GGCTCCGACG GCAGTGGTGA CGATGGACGA CCTCGCAAGC GCGGGCGGCC GACCATGAAG 
GAGAAGATCA CCGGATTCAC TGATGCGGAG TTGCGTCGCT TCATTCGCAG TTACAAAAAG 
TTTCCCGCTC CTCTCCACCG CATGGAGGCC ATCGCATGTG ATGCTGAGCT GCAGGAAAAG 
CCACTGGCGG AACTGAAGCG CCTCGGAGAG ATGCTGCATG ACCGCTGCGT TCAGTTCCTG 
CACGAGCACA AGGAGGAAGA GAGTAAGACT GCGGCGACGG ATGAGACGCC GGGCGCCAAG 
CAGCGCCGCG CACGCGCCAC CTTCTCCGTT AAGCTGGGTG GCGTCTCCTT TAATGCCAAA 
AAGCTGCTGG CCTGCGAACA GGAGCTTCAG CCGCTCAACG AGATCATGCC CAGCATGCCC 
GAAGAGCGCC AGCAATGGAG CTTCAATATC AAGACACGCG CCCCGGTCTT CGACGTGGAT 
TGGGGCATCG AGGAGGACAC GAAGCTACTG TGCGGCATTT ACCAGTACGG CATTGGATCC 
TGGGAGCAGA TGAAGCTAGA CCCCACGCTC AAACTGACCG ACAAGATTTT GCTTAACGAT 
ACGCGCAAGC CCCAGGCCAA GCAGCTGCAG ACGCGTGCCG AGTACCTGCT CAAGATCATC 
AAGAAGAACG TGGAGCTGAC CAAGGGAGGA CAACGCCGAC AACGGCGCCC CAGAGCATCG 
CGAGCTAACG ATGCCAAGGC GGCAAGCCAG TCTGCTAGTT CGACCATCGA TGCTAAGCCG 
CACGATGGCG AGGACGCTGC TGGCGACGCA CGCACCGTCG CCGAAAGCAG TAACAGTCAA 
GTAGATCCAT CTACCGCGTC GCCGCACAAT GCTCCAGCTA CGGAACAGCA CGGTGACCCT 
GCGAAGAAAG CCAAAAAATC CAAGGCTCGT TCGAAGAAAA CCAGCGCCTC GGACAACAAC 
GGCAACAAGC CGATGCACTT TACGGCGAAC AATGAACCTA GGGCTTTGGA GGTCCTGGGT 
GACCTAGATC CCAGCATTTT TAACGAGTGC AAAGAAAAAA TGCGGCCGGT GAAGAAGGCC 
CTCAAGGCGC TGGACCAGCC GGATGTCAGT CTTTCCGACC AGGATCAGCT GCAGCACACG 
AGGGATTGCC TGCTTCAAAT TGGCAAGCAG ATCGATGTTT GCCTCAATCC CTACGCCGAG 
ACAGAGAAAA AGGAATGGCG CAGTAACCTT TGGTACTTTG TGTCGAAATT CACTGAGCTG 
GATGCCAAGC GCCTATTCAA GATTTACAAA CACGCACTCA AGCAGAAAGC GGGCGGCGAT 
GGCGAGGCGA AGGGAAAAGA CAAGGGCTCG TCAGGAAGTC CGGCCAAGTC TAAACCCAAC 
GGTGTGACTA CTGAAGAAAA AGAAAAGGAG CGCGACAGGA GTGGGGGCAA AAAGAAGAAA 
AAGGACAAGG ACAAGGAACG CAGTGGACAG GCGCGGTATC CGGAAACGGG TATACCCACC 
TCCGGTCGTT ACGCTGACCC TCCCCTAAAG CGTAAGCGGG ATGAGAACGA CGCTGATGCA 
AGCAGCGGAC TAGCAGGTGC CCCCGGCGGA GGCATCGGCG ACAACCTCAA GTCCATGTCC 
TTCAAACGGC TGAACATGGA CCGTCACGAG GACCGTAAGA AGCACCATCG CGGTCCCGAC 
TACTACGGTG GCAGTGGCCC ACCCATGGGA AGTGGTAGCT ACGAAGGCGG CAGCAATTCC 
CGGCGCCAGG GCCCCACCTC CCCATCTACA CCCCGTACTG GCCGGGGCGG CTATGATCCA 
CCGCCCGCGC CATCCGGCTA CACGCCGGAA ATGGAGCGAT GGCAATCCCG CGATAGATAC 
AGCCAGGATT ACAAACGGGA TCGCTACGAT GGATATGGGC GCAGTGGGGG AGGTCAAGGC 
AGCTATCATC GCGAACGCGA CCGTCGTCCC GAGAAGCGCA GATATCCGTC TGGCTTGCCA 
CCGCATCCTT ACTCAAGCCA CTACCTGCCG CCCAATTACT ATGGATTGCC TAATGGAGCC 
GTTCCGGGCC TACCGCCACC GTCGTCTGTC TACCGCAGTG ACCCACGCGG ATATCCGGTC 
ATGCCGCGAG ACTATCCTGC CGACTACAGA CGCAGCGACT ACGAGCGGCG CACGCAGACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Chd1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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