Pde5a cDNA ORF clone, Mus musculus(house mouse)

The following Pde5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu02758 NM_153422.2
Latest version!
Mus musculus phosphodiesterase 5A, cGMP-specific (Pde5a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu31908 XM_006501481.3
Latest version!
Mus musculus phosphodiesterase 5A, cGMP-specific (Pde5a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
View Old Accession Versions >>
OMu31908 XM_006501481.2 Mus musculus phosphodiesterase 5A, cGMP-specific (Pde5a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu02758
    Clone ID Related Accession (Same CDS sequence) NM_153422.2
    Accession Version NM_153422.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2598bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Mus musculus(house mouse)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC101922.12, BC119137.1, AC155158.15 and BX631325.1. On Oct 1, 2009 this sequence version replaced NM_153422.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF541937.1, BC119137.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAACGAG CGGGCCCCAA CTCCGTGCGG TCGCAGCAGC AGCGGGACCC GGACTGGGTG 
    GAAGCGTGGC TGGATGATCA CCGGGACTTT ACCTTCTCTT ACTTTATTAG AAAGGCCACC
    AGAGACATGG TCAACGCATG GTTTTCAGAG AGAGTTCACA ACATCCCTGT GTGCAAGGAA
    GGCATCCGAG CCCATACCGA GTCCTGCTCT TGCTCTCTGC AGCAAAGTCC TCATGCAGAT
    AATACCACCC CTGGAGCACC AGCCAGAAAA ATATCTGCCT CTGAATTTGA CCGACCTCTT
    AGACCCATTG TTGTCAAGGA TTCCGAGGGA ACAGTGAGCT TTCTCTCTGA CTCAGGAAAA
    AAGGAACAAA TGCCACTAAC CCCCCCAAGA TTTGATAGTG ATGAAGGGGA CCAGTGCTCA
    AGGCTCTTGG AGCTAGTAAA AGATATTTCC AGTCATTTGG ATGTCACAGC ATTGTGTCAC
    AAAATTTTCT TGCATATCCA TGGACTCATC TCTGCGGATC GCTATTCCCT GTTCCTTGTC
    TGTGAGGACA GCTCTAAAGA CAAATTCCTC ATCAGCCGCC TCTTTGATGT TGCTGAAGGT
    TCAACATTGG AGGAAGCTTC AAATAACTGT ATCCGTTTAG AGTGGAACAA AGGCATTGTG
    GGACATGTGG CAGCTTTTGG CGAGCCCTTG AACATCAAAG ATGCCTATGA GGATCCCCGG
    TTCAATGCAG AAGTTGACCA AATTACAGGC TACAAGACAC AAAGTATCCT TTGTATGCCA
    ATTAAGAACC ACAGGGAAGA GGTTGTTGGT GTAGCTCAGG CCATCAACAA GAAATCAGGA
    AATGGTGGGA CCTTCACTGA AAAAGATGAA AAGGACTTTG CGGCCTACCT GGCATTCTGT
    GGTATTGTTC TTCACAATGC TCAGCTCTAT GAAACGTCAT TGCTGGAGAA CAAAAGAAAT
    CAGGTGTTAC TTGACCTTGC CAGCTTAATC TTTGAAGAGC AGCAGTCACT GGAAGTCATT
    CTGAAGAAGA TAGCGGCCAC TATCATCTCC TTCATGCAGG TGCAGAAGTG CACCATCTTC
    ATTGTGGATG AAGACTGTCC TGACTCTTTC TCTCGTGTGT TTCACATGGA GTGTGAGGAA
    GTAGGAAAAC CCTCTGATCC TTTAACGAGG GAGCAAGATG CAAACAAAAT CAACTACATG
    TATGCTCAGT ATGTCAAAAA CACGATGGAG CCACTTAATA TCCCAGATGT CACCAAGGAC
    AAAAGATTTC CCTGGACAAA TGAAAACATG GGACACGTGA ACACGCCCTG CATTGGAAGT
    TTGCTTTGCA CCCCTATAAA AAATGGGAAG AAGAACAAAG TCATAGGCGT CTGCCAACTT
    GTTAATAAGA TGGAGGAGAA TACTGGCAAG ATTAAAGCTT TCAACCAAAA TGATGAGCAG
    TTCCTGGAAG CCTTTGTCAT CTTCTGTGGC TTGGGGATCC AGAACACACA GATGTATGAA
    GCGGTGGAGA GAGCCATGGC CAAGCAGATG GTGACATTAG AGGTCCTGTC TTACCATGCA
    TCAGCGGCGG AGGAAGAAAC AAGAGAGCTC CAGGCTTTAT CGGCTGCTGT GGTACCATCG
    GCCCAAACCC TTAAAATTAC CGACTTCAGC TTCAGTGACT TTGAGCTGTC TGATCTGGAA
    ACAGCGCTGT GTACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG
    AAACATGAGG TTCTTTGCAG GTGGATTTTA AGTGTGAAGA AGAATTATCG GAAGAACGTT
    GCCTACCACA ATTGGAGGCA TGCCTTTAAC ACGGCACAGT GCATGTTTGC TGCTCTGAAA
    GCCGGCAAGA TCCAGAACAA GTTGACGGAT CTGGAGACCC TTGCGTTGCT CATTGCTGCT
    TTAAGCCATG ACTTGGATCA TCGTGGCGTG AACAACTCAT ACATACAGCG GAGCGAGCAC
    CCGCTGGCTC AGCTGTACTG TCACTCCATC ATGGAACACC ATCATTTTGA CCAGTGCTTG
    ATGATTCTAA ACAGCCCAGG CAACCAGATT CTCAGTGGCC TCTCCATTGA CGAATATAAG
    ACCACACTGA AAATAATCAA GCAAGCAATT TTAGCCACAG ACCTAGCACT GTACATTAAG
    AGACGAGGAG AATTTTTTGA ACTTATACGA AAAAATCAAT TCAGTTTTGA AGATCCTCTT
    CAAAAGGAGT TGTTTCTAGC AATGCTGATG ACAGCCTGCG ACCTCTCTGC GATTACTAAA
    CCCTGGCCTA TTCAACAACG GATAGCAGAA CTCGTGGCAG CTGAATTCTT TGATCAAGGA
    GACAGAGAGA GAAAAGAACT TAACATGGAG CCGGCTGATC TAATGAACAG AGAGAAGAAA
    AATAAAATCC CAAGTATGCA AGTTGGGTTC ATAGATGCCA TCTGCTTGCA GCTGTATGAG
    GCCCTGACCC ACGTATCCGA AGACTGTTTG CCCTTGCTGG ATGGCTGCAG GAAGAACAGA
    CAGAAATGGC AGGCCCTCGC GGAGCAGCAG GAGAAGATGC TCCTTAACGG GGAGAGCAGC
    CAGGGCAAGC GGGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_700471.2
    CDS89..2686
    Translation

    Target ORF information:

    RefSeq Version NM_153422.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus phosphodiesterase 5A, cGMP-specific (Pde5a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_153422.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAACGAG CGGGCCCCAA CTCCGTGCGG TCGCAGCAGC AGCGGGACCC GGACTGGGTG 
    GAAGCGTGGC TGGATGATCA CCGGGACTTT ACCTTCTCTT ACTTTATTAG AAAGGCCACC
    AGAGACATGG TCAACGCATG GTTTTCAGAG AGAGTTCACA ACATCCCTGT GTGCAAGGAA
    GGCATCCGAG CCCATACCGA GTCCTGCTCT TGCTCTCTGC AGCAAAGTCC TCATGCAGAT
    AATACCACCC CTGGAGCACC AGCCAGAAAA ATATCTGCCT CTGAATTTGA CCGACCTCTT
    AGACCCATTG TTGTCAAGGA TTCCGAGGGA ACAGTGAGCT TTCTCTCTGA CTCAGGAAAA
    AAGGAACAAA TGCCACTAAC CCCCCCAAGA TTTGATAGTG ATGAAGGGGA CCAGTGCTCA
    AGGCTCTTGG AGCTAGTAAA AGATATTTCC AGTCATTTGG ATGTCACAGC ATTGTGTCAC
    AAAATTTTCT TGCATATCCA TGGACTCATC TCTGCGGATC GCTATTCCCT GTTCCTTGTC
    TGTGAGGACA GCTCTAAAGA CAAATTCCTC ATCAGCCGCC TCTTTGATGT TGCTGAAGGT
    TCAACATTGG AGGAAGCTTC AAATAACTGT ATCCGTTTAG AGTGGAACAA AGGCATTGTG
    GGACATGTGG CAGCTTTTGG CGAGCCCTTG AACATCAAAG ATGCCTATGA GGATCCCCGG
    TTCAATGCAG AAGTTGACCA AATTACAGGC TACAAGACAC AAAGTATCCT TTGTATGCCA
    ATTAAGAACC ACAGGGAAGA GGTTGTTGGT GTAGCTCAGG CCATCAACAA GAAATCAGGA
    AATGGTGGGA CCTTCACTGA AAAAGATGAA AAGGACTTTG CGGCCTACCT GGCATTCTGT
    GGTATTGTTC TTCACAATGC TCAGCTCTAT GAAACGTCAT TGCTGGAGAA CAAAAGAAAT
    CAGGTGTTAC TTGACCTTGC CAGCTTAATC TTTGAAGAGC AGCAGTCACT GGAAGTCATT
    CTGAAGAAGA TAGCGGCCAC TATCATCTCC TTCATGCAGG TGCAGAAGTG CACCATCTTC
    ATTGTGGATG AAGACTGTCC TGACTCTTTC TCTCGTGTGT TTCACATGGA GTGTGAGGAA
    GTAGGAAAAC CCTCTGATCC TTTAACGAGG GAGCAAGATG CAAACAAAAT CAACTACATG
    TATGCTCAGT ATGTCAAAAA CACGATGGAG CCACTTAATA TCCCAGATGT CACCAAGGAC
    AAAAGATTTC CCTGGACAAA TGAAAACATG GGACACGTGA ACACGCCCTG CATTGGAAGT
    TTGCTTTGCA CCCCTATAAA AAATGGGAAG AAGAACAAAG TCATAGGCGT CTGCCAACTT
    GTTAATAAGA TGGAGGAGAA TACTGGCAAG ATTAAAGCTT TCAACCAAAA TGATGAGCAG
    TTCCTGGAAG CCTTTGTCAT CTTCTGTGGC TTGGGGATCC AGAACACACA GATGTATGAA
    GCGGTGGAGA GAGCCATGGC CAAGCAGATG GTGACATTAG AGGTCCTGTC TTACCATGCA
    TCAGCGGCGG AGGAAGAAAC AAGAGAGCTC CAGGCTTTAT CGGCTGCTGT GGTACCATCG
    GCCCAAACCC TTAAAATTAC CGACTTCAGC TTCAGTGACT TTGAGCTGTC TGATCTGGAA
    ACAGCGCTGT GTACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG
    AAACATGAGG TTCTTTGCAG GTGGATTTTA AGTGTGAAGA AGAATTATCG GAAGAACGTT
    GCCTACCACA ATTGGAGGCA TGCCTTTAAC ACGGCACAGT GCATGTTTGC TGCTCTGAAA
    GCCGGCAAGA TCCAGAACAA GTTGACGGAT CTGGAGACCC TTGCGTTGCT CATTGCTGCT
    TTAAGCCATG ACTTGGATCA TCGTGGCGTG AACAACTCAT ACATACAGCG GAGCGAGCAC
    CCGCTGGCTC AGCTGTACTG TCACTCCATC ATGGAACACC ATCATTTTGA CCAGTGCTTG
    ATGATTCTAA ACAGCCCAGG CAACCAGATT CTCAGTGGCC TCTCCATTGA CGAATATAAG
    ACCACACTGA AAATAATCAA GCAAGCAATT TTAGCCACAG ACCTAGCACT GTACATTAAG
    AGACGAGGAG AATTTTTTGA ACTTATACGA AAAAATCAAT TCAGTTTTGA AGATCCTCTT
    CAAAAGGAGT TGTTTCTAGC AATGCTGATG ACAGCCTGCG ACCTCTCTGC GATTACTAAA
    CCCTGGCCTA TTCAACAACG GATAGCAGAA CTCGTGGCAG CTGAATTCTT TGATCAAGGA
    GACAGAGAGA GAAAAGAACT TAACATGGAG CCGGCTGATC TAATGAACAG AGAGAAGAAA
    AATAAAATCC CAAGTATGCA AGTTGGGTTC ATAGATGCCA TCTGCTTGCA GCTGTATGAG
    GCCCTGACCC ACGTATCCGA AGACTGTTTG CCCTTGCTGG ATGGCTGCAG GAAGAACAGA
    CAGAAATGGC AGGCCCTCGC GGAGCAGCAG GAGAAGATGC TCCTTAACGG GGAGAGCAGC
    CAGGGCAAGC GGGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu31908
    Clone ID Related Accession (Same CDS sequence) XM_006501481.2 , XM_006501481.3
    Accession Version XM_006501481.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTGCCCT TTGGAGACAA AACGAGAGAC ATGGTCAACG CATGGTTTTC AGAGAGAGTT 
    CACAACATCC CTGTGTGCAA GGAAGGCATC CGAGCCCATA CCGAGTCCTG CTCTTGCTCT
    CTGCAGCAAA GTCCTCATGC AGATAATACC ACCCCTGGAG CACCAGCCAG AAAAATATCT
    GCCTCTGAAT TTGACCGACC TCTTAGACCC ATTGTTGTCA AGGATTCCGA GGGAACAGTG
    AGCTTTCTCT CTGACTCAGG AAAAAAGGAA CAAATGCCAC TAACCCCCCC AAGATTTGAT
    AGTGATGAAG GGGACCAGTG CTCAAGGCTC TTGGAGCTAG TAAAAGATAT TTCCAGTCAT
    TTGGATGTCA CAGCATTGTG TCACAAAATT TTCTTGCATA TCCATGGACT CATCTCTGCG
    GATCGCTATT CCCTGTTCCT TGTCTGTGAG GACAGCTCTA AAGACAAATT CCTCATCAGC
    CGCCTCTTTG ATGTTGCTGA AGGTTCAACA TTGGAGGAAG CTTCAAATAA CTGTATCCGT
    TTAGAGTGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCTT TTGGCGAGCC CTTGAACATC
    AAAGATGCCT ATGAGGATCC CCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG
    ACACAAAGTA TCCTTTGTAT GCCAATTAAG AACCACAGGG AAGAGGTTGT TGGTGTAGCT
    CAGGCCATCA ACAAGAAATC AGGAAATGGT GGGACCTTCA CTGAAAAAGA TGAAAAGGAC
    TTTGCGGCCT ACCTGGCATT CTGTGGTATT GTTCTTCACA ATGCTCAGCT CTATGAAACG
    TCATTGCTGG AGAACAAAAG AAATCAGGTG TTACTTGACC TTGCCAGCTT AATCTTTGAA
    GAGCAGCAGT CACTGGAAGT CATTCTGAAG AAGATAGCGG CCACTATCAT CTCCTTCATG
    CAGGTGCAGA AGTGCACCAT CTTCATTGTG GATGAAGACT GTCCTGACTC TTTCTCTCGT
    GTGTTTCACA TGGAGTGTGA GGAAGTAGGA AAACCCTCTG ATCCTTTAAC GAGGGAGCAA
    GATGCAAACA AAATCAACTA CATGTATGCT CAGTATGTCA AAAACACGAT GGAGCCACTT
    AATATCCCAG ATGTCACCAA GGACAAAAGA TTTCCCTGGA CAAATGAAAA CATGGGACAC
    GTGAACACGC CCTGCATTGG AAGTTTGCTT TGCACCCCTA TAAAAAATGG GAAGAAGAAC
    AAAGTCATAG GCGTCTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGATTAAA
    GCTTTCAACC AAAATGATGA GCAGTTCCTG GAAGCCTTTG TCATCTTCTG TGGCTTGGGG
    ATCCAGAACA CACAGATGTA TGAAGCGGTG GAGAGAGCCA TGGCCAAGCA GATGGTGACA
    TTAGAGGTCC TGTCTTACCA TGCATCAGCG GCGGAGGAAG AAACAAGAGA GCTCCAGGCT
    TTATCGGCTG CTGTGGTACC ATCGGCCCAA ACCCTTAAAA TTACCGACTT CAGCTTCAGT
    GACTTTGAGC TGTCTGATCT GGAAACAGCG CTGTGTACAA TTCGGATGTT CACTGACCTC
    AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGGTGGAT TTTAAGTGTG
    AAGAAGAATT ATCGGAAGAA CGTTGCCTAC CACAATTGGA GGCATGCCTT TAACACGGCA
    CAGTGCATGT TTGCTGCTCT GAAAGCCGGC AAGATCCAGA ACAAGTTGAC GGATCTGGAG
    ACCCTTGCGT TGCTCATTGC TGCTTTAAGC CATGACTTGG ATCATCGTGG CGTGAACAAC
    TCATACATAC AGCGGAGCGA GCACCCGCTG GCTCAGCTGT ACTGTCACTC CATCATGGAA
    CACCATCATT TTGACCAGTG CTTGATGATT CTAAACAGCC CAGGCAACCA GATTCTCAGT
    GGCCTCTCCA TTGACGAATA TAAGACCACA CTGAAAATAA TCAAGCAAGC AATTTTAGCC
    ACAGACCTAG CACTGTACAT TAAGAGACGA GGAGAATTTT TTGAACTTAT ACGAAAAAAT
    CAATTCAGTT TTGAAGATCC TCTTCAAAAG GAGTTGTTTC TAGCAATGCT GATGACAGCC
    TGCGACCTCT CTGCGATTAC TAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTCGTG
    GCAGCTGAAT TCTTTGATCA AGGAGACAGA GAGAGAAAAG AACTTAACAT GGAGCCGGCT
    GATCTAATGA ACAGAGAGAA GAAAAATAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT
    GCCATCTGCT TGCAGCTGTA TGAGGCCCTG ACCCACGTAT CCGAAGACTG TTTGCCCTTG
    CTGGATGGCT GCAGGAAGAA CAGACAGAAA TGGCAGGCCC TCGCGGAGCA GCAGGAGAAG
    ATGCTCCTTA ACGGGGAGAG CAGCCAGGGC AAGCGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006501544.1
    CDS1190..3691
    Translation

    Target ORF information:

    RefSeq Version XM_006501481.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus phosphodiesterase 5A, cGMP-specific (Pde5a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006501481.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTGCCCT TTGGAGACAA AACGAGAGAC ATGGTCAACG CATGGTTTTC AGAGAGAGTT 
    CACAACATCC CTGTGTGCAA GGAAGGCATC CGAGCCCATA CCGAGTCCTG CTCTTGCTCT
    CTGCAGCAAA GTCCTCATGC AGATAATACC ACCCCTGGAG CACCAGCCAG AAAAATATCT
    GCCTCTGAAT TTGACCGACC TCTTAGACCC ATTGTTGTCA AGGATTCCGA GGGAACAGTG
    AGCTTTCTCT CTGACTCAGG AAAAAAGGAA CAAATGCCAC TAACCCCCCC AAGATTTGAT
    AGTGATGAAG GGGACCAGTG CTCAAGGCTC TTGGAGCTAG TAAAAGATAT TTCCAGTCAT
    TTGGATGTCA CAGCATTGTG TCACAAAATT TTCTTGCATA TCCATGGACT CATCTCTGCG
    GATCGCTATT CCCTGTTCCT TGTCTGTGAG GACAGCTCTA AAGACAAATT CCTCATCAGC
    CGCCTCTTTG ATGTTGCTGA AGGTTCAACA TTGGAGGAAG CTTCAAATAA CTGTATCCGT
    TTAGAGTGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCTT TTGGCGAGCC CTTGAACATC
    AAAGATGCCT ATGAGGATCC CCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG
    ACACAAAGTA TCCTTTGTAT GCCAATTAAG AACCACAGGG AAGAGGTTGT TGGTGTAGCT
    CAGGCCATCA ACAAGAAATC AGGAAATGGT GGGACCTTCA CTGAAAAAGA TGAAAAGGAC
    TTTGCGGCCT ACCTGGCATT CTGTGGTATT GTTCTTCACA ATGCTCAGCT CTATGAAACG
    TCATTGCTGG AGAACAAAAG AAATCAGGTG TTACTTGACC TTGCCAGCTT AATCTTTGAA
    GAGCAGCAGT CACTGGAAGT CATTCTGAAG AAGATAGCGG CCACTATCAT CTCCTTCATG
    CAGGTGCAGA AGTGCACCAT CTTCATTGTG GATGAAGACT GTCCTGACTC TTTCTCTCGT
    GTGTTTCACA TGGAGTGTGA GGAAGTAGGA AAACCCTCTG ATCCTTTAAC GAGGGAGCAA
    GATGCAAACA AAATCAACTA CATGTATGCT CAGTATGTCA AAAACACGAT GGAGCCACTT
    AATATCCCAG ATGTCACCAA GGACAAAAGA TTTCCCTGGA CAAATGAAAA CATGGGACAC
    GTGAACACGC CCTGCATTGG AAGTTTGCTT TGCACCCCTA TAAAAAATGG GAAGAAGAAC
    AAAGTCATAG GCGTCTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGATTAAA
    GCTTTCAACC AAAATGATGA GCAGTTCCTG GAAGCCTTTG TCATCTTCTG TGGCTTGGGG
    ATCCAGAACA CACAGATGTA TGAAGCGGTG GAGAGAGCCA TGGCCAAGCA GATGGTGACA
    TTAGAGGTCC TGTCTTACCA TGCATCAGCG GCGGAGGAAG AAACAAGAGA GCTCCAGGCT
    TTATCGGCTG CTGTGGTACC ATCGGCCCAA ACCCTTAAAA TTACCGACTT CAGCTTCAGT
    GACTTTGAGC TGTCTGATCT GGAAACAGCG CTGTGTACAA TTCGGATGTT CACTGACCTC
    AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGGTGGAT TTTAAGTGTG
    AAGAAGAATT ATCGGAAGAA CGTTGCCTAC CACAATTGGA GGCATGCCTT TAACACGGCA
    CAGTGCATGT TTGCTGCTCT GAAAGCCGGC AAGATCCAGA ACAAGTTGAC GGATCTGGAG
    ACCCTTGCGT TGCTCATTGC TGCTTTAAGC CATGACTTGG ATCATCGTGG CGTGAACAAC
    TCATACATAC AGCGGAGCGA GCACCCGCTG GCTCAGCTGT ACTGTCACTC CATCATGGAA
    CACCATCATT TTGACCAGTG CTTGATGATT CTAAACAGCC CAGGCAACCA GATTCTCAGT
    GGCCTCTCCA TTGACGAATA TAAGACCACA CTGAAAATAA TCAAGCAAGC AATTTTAGCC
    ACAGACCTAG CACTGTACAT TAAGAGACGA GGAGAATTTT TTGAACTTAT ACGAAAAAAT
    CAATTCAGTT TTGAAGATCC TCTTCAAAAG GAGTTGTTTC TAGCAATGCT GATGACAGCC
    TGCGACCTCT CTGCGATTAC TAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTCGTG
    GCAGCTGAAT TCTTTGATCA AGGAGACAGA GAGAGAAAAG AACTTAACAT GGAGCCGGCT
    GATCTAATGA ACAGAGAGAA GAAAAATAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT
    GCCATCTGCT TGCAGCTGTA TGAGGCCCTG ACCCACGTAT CCGAAGACTG TTTGCCCTTG
    CTGGATGGCT GCAGGAAGAA CAGACAGAAA TGGCAGGCCC TCGCGGAGCA GCAGGAGAAG
    ATGCTCCTTA ACGGGGAGAG CAGCCAGGGC AAGCGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu31908
    Clone ID Related Accession (Same CDS sequence) XM_006501481.2 , XM_006501481.3
    Accession Version XM_006501481.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501481.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTGCCCT TTGGAGACAA AACGAGAGAC ATGGTCAACG CATGGTTTTC AGAGAGAGTT 
    CACAACATCC CTGTGTGCAA GGAAGGCATC CGAGCCCATA CCGAGTCCTG CTCTTGCTCT
    CTGCAGCAAA GTCCTCATGC AGATAATACC ACCCCTGGAG CACCAGCCAG AAAAATATCT
    GCCTCTGAAT TTGACCGACC TCTTAGACCC ATTGTTGTCA AGGATTCCGA GGGAACAGTG
    AGCTTTCTCT CTGACTCAGG AAAAAAGGAA CAAATGCCAC TAACCCCCCC AAGATTTGAT
    AGTGATGAAG GGGACCAGTG CTCAAGGCTC TTGGAGCTAG TAAAAGATAT TTCCAGTCAT
    TTGGATGTCA CAGCATTGTG TCACAAAATT TTCTTGCATA TCCATGGACT CATCTCTGCG
    GATCGCTATT CCCTGTTCCT TGTCTGTGAG GACAGCTCTA AAGACAAATT CCTCATCAGC
    CGCCTCTTTG ATGTTGCTGA AGGTTCAACA TTGGAGGAAG CTTCAAATAA CTGTATCCGT
    TTAGAGTGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCTT TTGGCGAGCC CTTGAACATC
    AAAGATGCCT ATGAGGATCC CCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG
    ACACAAAGTA TCCTTTGTAT GCCAATTAAG AACCACAGGG AAGAGGTTGT TGGTGTAGCT
    CAGGCCATCA ACAAGAAATC AGGAAATGGT GGGACCTTCA CTGAAAAAGA TGAAAAGGAC
    TTTGCGGCCT ACCTGGCATT CTGTGGTATT GTTCTTCACA ATGCTCAGCT CTATGAAACG
    TCATTGCTGG AGAACAAAAG AAATCAGGTG TTACTTGACC TTGCCAGCTT AATCTTTGAA
    GAGCAGCAGT CACTGGAAGT CATTCTGAAG AAGATAGCGG CCACTATCAT CTCCTTCATG
    CAGGTGCAGA AGTGCACCAT CTTCATTGTG GATGAAGACT GTCCTGACTC TTTCTCTCGT
    GTGTTTCACA TGGAGTGTGA GGAAGTAGGA AAACCCTCTG ATCCTTTAAC GAGGGAGCAA
    GATGCAAACA AAATCAACTA CATGTATGCT CAGTATGTCA AAAACACGAT GGAGCCACTT
    AATATCCCAG ATGTCACCAA GGACAAAAGA TTTCCCTGGA CAAATGAAAA CATGGGACAC
    GTGAACACGC CCTGCATTGG AAGTTTGCTT TGCACCCCTA TAAAAAATGG GAAGAAGAAC
    AAAGTCATAG GCGTCTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGATTAAA
    GCTTTCAACC AAAATGATGA GCAGTTCCTG GAAGCCTTTG TCATCTTCTG TGGCTTGGGG
    ATCCAGAACA CACAGATGTA TGAAGCGGTG GAGAGAGCCA TGGCCAAGCA GATGGTGACA
    TTAGAGGTCC TGTCTTACCA TGCATCAGCG GCGGAGGAAG AAACAAGAGA GCTCCAGGCT
    TTATCGGCTG CTGTGGTACC ATCGGCCCAA ACCCTTAAAA TTACCGACTT CAGCTTCAGT
    GACTTTGAGC TGTCTGATCT GGAAACAGCG CTGTGTACAA TTCGGATGTT CACTGACCTC
    AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGGTGGAT TTTAAGTGTG
    AAGAAGAATT ATCGGAAGAA CGTTGCCTAC CACAATTGGA GGCATGCCTT TAACACGGCA
    CAGTGCATGT TTGCTGCTCT GAAAGCCGGC AAGATCCAGA ACAAGTTGAC GGATCTGGAG
    ACCCTTGCGT TGCTCATTGC TGCTTTAAGC CATGACTTGG ATCATCGTGG CGTGAACAAC
    TCATACATAC AGCGGAGCGA GCACCCGCTG GCTCAGCTGT ACTGTCACTC CATCATGGAA
    CACCATCATT TTGACCAGTG CTTGATGATT CTAAACAGCC CAGGCAACCA GATTCTCAGT
    GGCCTCTCCA TTGACGAATA TAAGACCACA CTGAAAATAA TCAAGCAAGC AATTTTAGCC
    ACAGACCTAG CACTGTACAT TAAGAGACGA GGAGAATTTT TTGAACTTAT ACGAAAAAAT
    CAATTCAGTT TTGAAGATCC TCTTCAAAAG GAGTTGTTTC TAGCAATGCT GATGACAGCC
    TGCGACCTCT CTGCGATTAC TAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTCGTG
    GCAGCTGAAT TCTTTGATCA AGGAGACAGA GAGAGAAAAG AACTTAACAT GGAGCCGGCT
    GATCTAATGA ACAGAGAGAA GAAAAATAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT
    GCCATCTGCT TGCAGCTGTA TGAGGCCCTG ACCCACGTAT CCGAAGACTG TTTGCCCTTG
    CTGGATGGCT GCAGGAAGAA CAGACAGAAA TGGCAGGCCC TCGCGGAGCA GCAGGAGAAG
    ATGCTCCTTA ACGGGGAGAG CAGCCAGGGC AAGCGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006501544.1
    CDS246..2747
    Translation

    Target ORF information:

    RefSeq Version XM_006501481.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus phosphodiesterase 5A, cGMP-specific (Pde5a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006501481.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTGCCCT TTGGAGACAA AACGAGAGAC ATGGTCAACG CATGGTTTTC AGAGAGAGTT 
    CACAACATCC CTGTGTGCAA GGAAGGCATC CGAGCCCATA CCGAGTCCTG CTCTTGCTCT
    CTGCAGCAAA GTCCTCATGC AGATAATACC ACCCCTGGAG CACCAGCCAG AAAAATATCT
    GCCTCTGAAT TTGACCGACC TCTTAGACCC ATTGTTGTCA AGGATTCCGA GGGAACAGTG
    AGCTTTCTCT CTGACTCAGG AAAAAAGGAA CAAATGCCAC TAACCCCCCC AAGATTTGAT
    AGTGATGAAG GGGACCAGTG CTCAAGGCTC TTGGAGCTAG TAAAAGATAT TTCCAGTCAT
    TTGGATGTCA CAGCATTGTG TCACAAAATT TTCTTGCATA TCCATGGACT CATCTCTGCG
    GATCGCTATT CCCTGTTCCT TGTCTGTGAG GACAGCTCTA AAGACAAATT CCTCATCAGC
    CGCCTCTTTG ATGTTGCTGA AGGTTCAACA TTGGAGGAAG CTTCAAATAA CTGTATCCGT
    TTAGAGTGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCTT TTGGCGAGCC CTTGAACATC
    AAAGATGCCT ATGAGGATCC CCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG
    ACACAAAGTA TCCTTTGTAT GCCAATTAAG AACCACAGGG AAGAGGTTGT TGGTGTAGCT
    CAGGCCATCA ACAAGAAATC AGGAAATGGT GGGACCTTCA CTGAAAAAGA TGAAAAGGAC
    TTTGCGGCCT ACCTGGCATT CTGTGGTATT GTTCTTCACA ATGCTCAGCT CTATGAAACG
    TCATTGCTGG AGAACAAAAG AAATCAGGTG TTACTTGACC TTGCCAGCTT AATCTTTGAA
    GAGCAGCAGT CACTGGAAGT CATTCTGAAG AAGATAGCGG CCACTATCAT CTCCTTCATG
    CAGGTGCAGA AGTGCACCAT CTTCATTGTG GATGAAGACT GTCCTGACTC TTTCTCTCGT
    GTGTTTCACA TGGAGTGTGA GGAAGTAGGA AAACCCTCTG ATCCTTTAAC GAGGGAGCAA
    GATGCAAACA AAATCAACTA CATGTATGCT CAGTATGTCA AAAACACGAT GGAGCCACTT
    AATATCCCAG ATGTCACCAA GGACAAAAGA TTTCCCTGGA CAAATGAAAA CATGGGACAC
    GTGAACACGC CCTGCATTGG AAGTTTGCTT TGCACCCCTA TAAAAAATGG GAAGAAGAAC
    AAAGTCATAG GCGTCTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGATTAAA
    GCTTTCAACC AAAATGATGA GCAGTTCCTG GAAGCCTTTG TCATCTTCTG TGGCTTGGGG
    ATCCAGAACA CACAGATGTA TGAAGCGGTG GAGAGAGCCA TGGCCAAGCA GATGGTGACA
    TTAGAGGTCC TGTCTTACCA TGCATCAGCG GCGGAGGAAG AAACAAGAGA GCTCCAGGCT
    TTATCGGCTG CTGTGGTACC ATCGGCCCAA ACCCTTAAAA TTACCGACTT CAGCTTCAGT
    GACTTTGAGC TGTCTGATCT GGAAACAGCG CTGTGTACAA TTCGGATGTT CACTGACCTC
    AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGGTGGAT TTTAAGTGTG
    AAGAAGAATT ATCGGAAGAA CGTTGCCTAC CACAATTGGA GGCATGCCTT TAACACGGCA
    CAGTGCATGT TTGCTGCTCT GAAAGCCGGC AAGATCCAGA ACAAGTTGAC GGATCTGGAG
    ACCCTTGCGT TGCTCATTGC TGCTTTAAGC CATGACTTGG ATCATCGTGG CGTGAACAAC
    TCATACATAC AGCGGAGCGA GCACCCGCTG GCTCAGCTGT ACTGTCACTC CATCATGGAA
    CACCATCATT TTGACCAGTG CTTGATGATT CTAAACAGCC CAGGCAACCA GATTCTCAGT
    GGCCTCTCCA TTGACGAATA TAAGACCACA CTGAAAATAA TCAAGCAAGC AATTTTAGCC
    ACAGACCTAG CACTGTACAT TAAGAGACGA GGAGAATTTT TTGAACTTAT ACGAAAAAAT
    CAATTCAGTT TTGAAGATCC TCTTCAAAAG GAGTTGTTTC TAGCAATGCT GATGACAGCC
    TGCGACCTCT CTGCGATTAC TAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTCGTG
    GCAGCTGAAT TCTTTGATCA AGGAGACAGA GAGAGAAAAG AACTTAACAT GGAGCCGGCT
    GATCTAATGA ACAGAGAGAA GAAAAATAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT
    GCCATCTGCT TGCAGCTGTA TGAGGCCCTG ACCCACGTAT CCGAAGACTG TTTGCCCTTG
    CTGGATGGCT GCAGGAAGAA CAGACAGAAA TGGCAGGCCC TCGCGGAGCA GCAGGAGAAG
    ATGCTCCTTA ACGGGGAGAG CAGCCAGGGC AAGCGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.