pde5ab cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pde5ab
Entrez Gene ID	553270
Full Name	phosphodiesterase 5A, cGMP-specific, b
Synonyms	PDE5,pde5a
General protein information	Preferred Names phosphodiesterase 5A, cGMP-specific, b Names cGMP-specific 3',5'-cyclic phosphodiesterase phosphodiesterase 5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009303486.2	XP_009301761.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
XM_009303486.3	XP_009301761.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
XM_005166563.3	XP_005166620.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
XM_005166563.4	XP_005166620.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
NM_001123260.1	NP_001116732.1	cGMP-specific 3',5'-cyclic phosphodiesterase

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pde5ab	NP_001116732.1
Drosophila melanogaster (fruit fly)	Pde11	NP_001188837.1
Pan troglodytes (chimpanzee)	PDE5A	XP_001150967.2
	PDE5A	XP_001099652.1
Canis lupus familiaris (dog)	PDE5A	NP_001003188.1
Bos taurus (cattle)	PDE5A	NP_776842.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008646	XP_314743.3
Rattus norvegicus (Norway rat)	Pde5a	NP_598268.1
Xenopus tropicalis (tropical clawed frog)	pde5a	NP_001072812.1
Homo sapiens (human)	PDE5A	NP_001074.2
Mus musculus (house mouse)	Pde5a	NP_700471.2
Gallus gallus (chicken)	PDE5A	XP_420631.3

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Identification of amino acid residues responsible for the selectivity of tadalafil binding to two closely related phosphodiesterases, PDE5 and PDE6.
Cahill KB, Quade JH, Carleton KL, Cote RH
The Journal of biological chemistry287(49)41406-16(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pde5ab gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pde5ab cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa32810	NM_001123260.1 Latest version!	Danio rerio phosphodiesterase 5A, cGMP-specific, b (pde5ab), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa13495	XM_005166563.4 Latest version!	Danio rerio phosphodiesterase 5A, cGMP-specific, b (pde5ab), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa41835	XM_009303486.3 Latest version!	Danio rerio phosphodiesterase 5A, cGMP-specific, b (pde5ab), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa41835	XM_009303486.2	Danio rerio phosphodiesterase 5A, cGMP-specific, b (pde5ab), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa13495	XM_005166563.3	Danio rerio phosphodiesterase 5A, cGMP-specific, b (pde5ab), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pde5ab ( NM_001123260.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001116732.1 Danio rerio phosphodiesterase 5A, cGMP-specific, b (pde5ab), mRNA.

Price & Availability↑

CloneID	ODa32810
Clone ID Related Accession (Same CDS sequence)	NM_001123260.1
Accession Version	NM_001123260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX663604.9 and BX664603.6. On Apr 11, 2008 this sequence version replaced XM_684577.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGTCT ATCCTTCCGA TAGTGAAAGG ATGATGGAGG CTTGGCTTGA CGACAACCTA 
GAGTTCGCAC ATTCTTACTT TGTACGCAAG GCTTCCCGGG AAATGGTCAA TGCCTGGTTT 
GCTGAGCGTG TACACTCCAT TCAACCATCT AGGGAACCAT CAAAAGCTGT GCCAGAACCT 
GTAACAGCAG TCCATTCTCT TGGTCCAACT GACGTGGTGC CATCTTCAAG TCCAGCTCCA 
TCCACCCCAA CACGAAAGAT CTCTGCATCT GAGTTTGACA GGCCGCTTCG CCCCATAGTC 
GTGAAAGACG CAGAGGGTTC CTTCACTTTC CTGAGTGACA CAGAAAGGGT GCAGATGCCG 
CTTCCTCCTT CCCAGCGGCC AGGCTCAGAC AATGGGACAG ATGAGGGTGG AGGTATTAGA 
GATCGTGGTA ATGGGGACCG ATGTGCTCGT CTGTTGGAGC TTGTACGTGA TGTTTCAAGT 
CATTTGGACC TTACTGCCCT CTGCCACAAG ATCTTTTTGC ATACCCATGA GCTGATAGCT 
GCAGATCGCT ACTCGTTGTT CTTGGTTGGG GAGGACAGTT CAAACAGAAA GTTCTTAGTT 
AGCAGGCTTT TCGATGTAGC AGAAGGATCT ACTCTTGAGG AGTCTTCAAA CAGTGGCATC 
CGCCTCGAAT GGAACAAAGG AATTGTGGGA TACGTTGCAG CCACTGGACA GCCTCTCAAC 
ATCAAGAATG CATATGAGGA TTCACGCTTT AATGCAGAAG TGGACCAAAT TACAGGCTAT 
AAGACACAGA GCATCTTGTG TTTGCCCATC AAAAACCACA GAGATGAGGT TGTTGGTGTA 
GCTCAAGCGA TCAATAAAAA GTGTGGAGAG AACAGCACCT TTACAGAACA GGATGAGAAG 
GACTTTTCTT CGTATCTGGC TTTCTCTGGC ATAGTGCTAC ACAATGCCCA ACTCTATGAG 
ACATCACAGC TAGAGAACAG GCGAAACCAG GTGTTGCTGG ATCTGGCTAG TCTGATCTTT 
GAGGAGCAGC AGTCACTGGA GGTCTTACTG AGGAAAACTG TGGCCACCAT CTTATCTTTC 
ATGCAAGCCC AAGAATGCAC AGTGTTCATT TCTGACAGAG AAACAGCTAA CACATTTGCC 
AGTGTGTTTC ACATGGAATA TGAGGAGATT GCTGGATCCA CAGACTTCCC AAACAGAGAA 
TGTGACGTCA ACAAGATTAA CTACATGTAC GCTCAGTATG TCAAAAACAC TATGGAGACA 
CTGAACATCA CAGATACCAC CAAAGATCAG CTATTTTCAT GGACAAGTGA AGATCCACAG 
TCCACGAACT GTCAAATCAA AAGTCTGCTG TGCACACCAA TAAGGAATGG GAAGAAAGAC 
AAAGTAATAG GCGTCTGCCA GTTAGTCAAT AAGATGGATG AAGCTTCAGG GGAGGTGAAA 
GCCTTTAATA GGAATGATGA GCAGTTTCTA GAGGCCTTTG CAGTCTTCTG TGGCCTGGGC 
ATCCAGAACA CACAGATGTA TGAAGCAGTG GAAAGAGCAA TGGCCAAGCA AGAGGTCACA 
CTTGAGGTCC TCTCTTATCA TGCCTCCGCT GCGGAGGAAG AGACGCGGGC GCTCCAGGTA 
ACGGCAGCTA CGATCCCCTC CGCTCAGTCT CTCAGACTGA TGGACTACAG TTTCAGTGAT 
TTTGACCTGA CAGATGCTGA GACGACCCAG GCAACCATCC GCATGTTTGT GGACCTCAAG 
CTTGTGCAGA ATTTTCAGAT AAAGTACAAG AGTTTGTGTC AGTGGATCTT AAGTGTCAAG 
AAGAACTACA GAAAGAATGT GGTCTACCAC AACTGGAGAC ATGCCTTCAA CACCTCCCAG 
TGCATGTTTG CTGTGCTGAA GTCTGGCCGT GTTCAGAATA ATCTGAGTGA TTTGGAGACT 
TTAGCTTTAA TGATTGCAAC ATTGAGTCAT GATTTGGACC ACCGAGGGGT CAACAACTCT 
TACATAAAAA GAAGTGACCA TCCACTGGCC CAACTGTACT GCCACTCCAC AATGGAGCAT 
CACCACTTTG ACCAGTGTCT CATGATTCTC AACAGCCCTG GTAATCAGAT TCTGAGTGGT 
CTTTCCCTGG ATGAGTACAA GGCGACACTG AAGATGATTG AGAAGGCTAT TTTGGCCACT 
GACCTGGCTG TCTACATGAA AAAAAGAACA GAATTCTTCG AGCTAGCTAA GAACAGTCAG 
TTTGAATGGG AAGATGATTG CCACAGGGAT CTATTAAGGT CGATGCTGAT GACAGCATGC 
GATATATCTG CTATTACAAA ACCATGGCCA GTTCAGAAGA AGATAGCAGA ACTGGTTGCC 
ACGGAGTTCT TTGAGCAAGG CGATAAGGAG CGGAGAGAGC TAAACATTGA ACCTATTGAT 
CTGATGAACA GGGAGAAACA GGATAAAATT CCCAGCATGC AAGTGTCATT TATTGATGCC 
ATTTGTACAC AGTTATATGA GGCTCTGACT GGGATCTCTG AGTTCTGCTC CCCCCTGCTG 
GAGGGCTGCC GGAAGAACAG GCAGAATTGG AAGATTCTTG CTGAACAAGG AGAGGAAGCA 
GCGCTGCTAA ATGGAGAACT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116732.1
CDS	1..2604
Translation	MEVYPSDSERMMEAWLDDNLEFAHSYFVRKASREMVNAWFAERVHSIQPSREPSKAVPEPVTAVHSLGPTDVVPSSSPAPSTPTRKISASEFDRPLRPIVVKDAEGSFTFLSDTERVQMPLPPSQRPGSDNGTDEGGGIRDRGNGDRCARLLELVRDVSSHLDLTALCHKIFLHTHELIAADRYSLFLVGEDSSNRKFLVSRLFDVAEGSTLEESSNSGIRLEWNKGIVGYVAATGQPLNIKNAYEDSRFNAEVDQITGYKTQSILCLPIKNHRDEVVGVAQAINKKCGENSTFTEQDEKDFSSYLAFSGIVLHNAQLYETSQLENRRNQVLLDLASLIFEEQQSLEVLLRKTVATILSFMQAQECTVFISDRETANTFASVFHMEYEEIAGSTDFPNRECDVNKINYMYAQYVKNTMETLNITDTTKDQLFSWTSEDPQSTNCQIKSLLCTPIRNGKKDKVIGVCQLVNKMDEASGEVKAFNRNDEQFLEAFAVFCGLGIQNTQMYEAVERAMAKQEVTLEVLSYHASAAEEETRALQVTAATIPSAQSLRLMDYSFSDFDLTDAETTQATIRMFVDLKLVQNFQIKYKSLCQWILSVKKNYRKNVVYHNWRHAFNTSQCMFAVLKSGRVQNNLSDLETLALMIATLSHDLDHRGVNNSYIKRSDHPLAQLYCHSTMEHHHFDQCLMILNSPGNQILSGLSLDEYKATLKMIEKAILATDLAVYMKKRTEFFELAKNSQFEWEDDCHRDLLRSMLMTACDISAITKPWPVQKKIAELVATEFFEQGDKERRELNIEPIDLMNREKQDKIPSMQVSFIDAICTQLYEALTGISEFCSPLLEGCRKNRQNWKILAEQGEEAALLNGEL

Target ORF information:

RefSeq Version	NM_001123260.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphodiesterase 5A, cGMP-specific, b (pde5ab), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123260.1

ORF Insert Sequence:

ATGGAGGTCT ATCCTTCCGA TAGTGAAAGG ATGATGGAGG CTTGGCTTGA CGACAACCTA 
GAGTTCGCAC ATTCTTACTT TGTACGCAAG GCTTCCCGGG AAATGGTCAA TGCCTGGTTT 
GCTGAGCGTG TACACTCCAT TCAACCATCT AGGGAACCAT CAAAAGCTGT GCCAGAACCT 
GTAACAGCAG TCCATTCTCT TGGTCCAACT GACGTGGTGC CATCTTCAAG TCCAGCTCCA 
TCCACCCCAA CACGAAAGAT CTCTGCATCT GAGTTTGACA GGCCGCTTCG CCCCATAGTC 
GTGAAAGACG CAGAGGGTTC CTTCACTTTC CTGAGTGACA CAGAAAGGGT GCAGATGCCG 
CTTCCTCCTT CCCAGCGGCC AGGCTCAGAC AATGGGACAG ATGAGGGTGG AGGTATTAGA 
GATCGTGGTA ATGGGGACCG ATGTGCTCGT CTGTTGGAGC TTGTACGTGA TGTTTCAAGT 
CATTTGGACC TTACTGCCCT CTGCCACAAG ATCTTTTTGC ATACCCATGA GCTGATAGCT 
GCAGATCGCT ACTCGTTGTT CTTGGTTGGG GAGGACAGTT CAAACAGAAA GTTCTTAGTT 
AGCAGGCTTT TCGATGTAGC AGAAGGATCT ACTCTTGAGG AGTCTTCAAA CAGTGGCATC 
CGCCTCGAAT GGAACAAAGG AATTGTGGGA TACGTTGCAG CCACTGGACA GCCTCTCAAC 
ATCAAGAATG CATATGAGGA TTCACGCTTT AATGCAGAAG TGGACCAAAT TACAGGCTAT 
AAGACACAGA GCATCTTGTG TTTGCCCATC AAAAACCACA GAGATGAGGT TGTTGGTGTA 
GCTCAAGCGA TCAATAAAAA GTGTGGAGAG AACAGCACCT TTACAGAACA GGATGAGAAG 
GACTTTTCTT CGTATCTGGC TTTCTCTGGC ATAGTGCTAC ACAATGCCCA ACTCTATGAG 
ACATCACAGC TAGAGAACAG GCGAAACCAG GTGTTGCTGG ATCTGGCTAG TCTGATCTTT 
GAGGAGCAGC AGTCACTGGA GGTCTTACTG AGGAAAACTG TGGCCACCAT CTTATCTTTC 
ATGCAAGCCC AAGAATGCAC AGTGTTCATT TCTGACAGAG AAACAGCTAA CACATTTGCC 
AGTGTGTTTC ACATGGAATA TGAGGAGATT GCTGGATCCA CAGACTTCCC AAACAGAGAA 
TGTGACGTCA ACAAGATTAA CTACATGTAC GCTCAGTATG TCAAAAACAC TATGGAGACA 
CTGAACATCA CAGATACCAC CAAAGATCAG CTATTTTCAT GGACAAGTGA AGATCCACAG 
TCCACGAACT GTCAAATCAA AAGTCTGCTG TGCACACCAA TAAGGAATGG GAAGAAAGAC 
AAAGTAATAG GCGTCTGCCA GTTAGTCAAT AAGATGGATG AAGCTTCAGG GGAGGTGAAA 
GCCTTTAATA GGAATGATGA GCAGTTTCTA GAGGCCTTTG CAGTCTTCTG TGGCCTGGGC 
ATCCAGAACA CACAGATGTA TGAAGCAGTG GAAAGAGCAA TGGCCAAGCA AGAGGTCACA 
CTTGAGGTCC TCTCTTATCA TGCCTCCGCT GCGGAGGAAG AGACGCGGGC GCTCCAGGTA 
ACGGCAGCTA CGATCCCCTC CGCTCAGTCT CTCAGACTGA TGGACTACAG TTTCAGTGAT 
TTTGACCTGA CAGATGCTGA GACGACCCAG GCAACCATCC GCATGTTTGT GGACCTCAAG 
CTTGTGCAGA ATTTTCAGAT AAAGTACAAG AGTTTGTGTC AGTGGATCTT AAGTGTCAAG 
AAGAACTACA GAAAGAATGT GGTCTACCAC AACTGGAGAC ATGCCTTCAA CACCTCCCAG 
TGCATGTTTG CTGTGCTGAA GTCTGGCCGT GTTCAGAATA ATCTGAGTGA TTTGGAGACT 
TTAGCTTTAA TGATTGCAAC ATTGAGTCAT GATTTGGACC ACCGAGGGGT CAACAACTCT 
TACATAAAAA GAAGTGACCA TCCACTGGCC CAACTGTACT GCCACTCCAC AATGGAGCAT 
CACCACTTTG ACCAGTGTCT CATGATTCTC AACAGCCCTG GTAATCAGAT TCTGAGTGGT 
CTTTCCCTGG ATGAGTACAA GGCGACACTG AAGATGATTG AGAAGGCTAT TTTGGCCACT 
GACCTGGCTG TCTACATGAA AAAAAGAACA GAATTCTTCG AGCTAGCTAA GAACAGTCAG 
TTTGAATGGG AAGATGATTG CCACAGGGAT CTATTAAGGT CGATGCTGAT GACAGCATGC 
GATATATCTG CTATTACAAA ACCATGGCCA GTTCAGAAGA AGATAGCAGA ACTGGTTGCC 
ACGGAGTTCT TTGAGCAAGG CGATAAGGAG CGGAGAGAGC TAAACATTGA ACCTATTGAT 
CTGATGAACA GGGAGAAACA GGATAAAATT CCCAGCATGC AAGTGTCATT TATTGATGCC 
ATTTGTACAC AGTTATATGA GGCTCTGACT GGGATCTCTG AGTTCTGCTC CCCCCTGCTG 
GAGGGCTGCC GGAAGAACAG GCAGAATTGG AAGATTCTTG CTGAACAAGG AGAGGAAGCA 
GCGCTGCTAA ATGGAGAACT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde5ab ( XM_005166563.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166620.1 Danio rerio phosphodiesterase 5A, cGMP-specific, b (pde5ab), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa13495
Clone ID Related Accession (Same CDS sequence)	XM_005166563.3 , XM_005166563.4
Accession Version	XM_005166563.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166563.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTCT ATCCTTCCGA TAGTGAAAGG ATGATGGAGG CTTGGCTTGA CGACAACCTA 
GAGTTCGCAC ATTCTTACTT TGTACGCAAG GCTTCCCGGG AAATGGTCAA TGCCTGGTTT 
GCTGAGCGTG TACACTCCAT TCAACCATCT AGGGAACCAT CAAAAGCTGT GCCAGAACCT 
GTAACAGCAG TCCATTCTCT TGGTCCAACT GACGTGGTGC CATCTTCAAG TCCAGCTCCA 
TCCACCCCAA CACGAAAGAT CTCTGCATCT GAGTTTGACA GGCCGCTTCG CCCCATAGTC 
GTGAAAGACG CAGAGGGTTC CTTCACTTTC CTGAGTGACA CAGAAAGGGT GCAGATGCCG 
CTTCCTCCTT CCCAGCGGCC AGGCTCAGAC AATGGGACAG ATGAGGGTGG AGGTATTAGA 
GATCGTGGTA ATGGGGACCG ATGTGCTCGT CTGTTGGAGC TTGTACGTGA TGTTTCAAGT 
CATTTGGACC TTACTGCCCT CTGCCACAAG ATCTTTTTGC ATACCCATGA GCTGATAGCT 
GCAGATCGCT ACTCGTTGTT CTTGGTTGGG GAGGACAGTT CAAACAGAAA GTTCTTAGTT 
AGCAGGCTTT TCGATGTAGC AGAAGGATCT ACTCTTGAGG AGTCTTCAAA CAGTGGCATC 
CGCCTCGAAT GGAACAAAGG AATTGTGGGA TACGTTGCAG CCACTGGACA GCCTCTCAAC 
ATCAAGAATG CATATGAGGA TTCACGCTTT AATGCAGAAG TGGACCAAAT TACAGGCTAT 
AAGACACAGA GCATCTTGTG TTTGCCCATC AAAAACCACA GAGATGAGGT TGTTGGTGTA 
GCTCAAGCGA TCAATAAAAA GTGTGGAGAG AACAGCACCT TTACAGAACA GGATGAGAAG 
GACTTTTCTT CGTATCTGGC TTTCTCTGGC ATAGTGCTAC ACAATGCCCA ACTCTATGAG 
ACATCACAGC TAGAGAACAG GCGAAACCAG GTGTTGCTGG ATCTGGCTAG TCTGATCTTT 
GAGGAGCAGC AGTCACTGGA GGTCTTACTG AGGAAAACTG TGGCCACCAT CTTATCTTTC 
ATGCAAGCCC AAGAATGCAC AGTGTTCATT TCTGACAGAG AAACAGCTAA CACATTTGCC 
AGTGTGTTTC ACATGGAATA TGAGGAGATT GCTGGATCCA CAGACTTCCC AAACAGAGAA 
TGTGACGTCA ACAAGATTAA CTACATGTAC GCTCAGTATG TCAAAAACAC TATGGAGACA 
CTGAACATCA CAGATACCAC CAAAGATCAG CTATTTTCAT GGACAAGTGA AGATCCACAG 
TCCACGAACT GTCAAATCAA AAGTCTGCTG TGCACACCAA TAAGGAATGG GAAGAAAGAC 
AAAGTAATAG GCGTCTGCCA GTTAGTCAAT AAGATGGATG AAGCTTCAGG GGAGGTGAAA 
GCCTTTAATA GGAATGATGA GCAGTTTCTA GAGGCCTTTG CAGTCTTCTG TGGCCTGGGC 
ATCCAGAACA CACAGATGTA TGAAGCAGTG GAAAGAGCAA TGGCCAAGCA AGAGGTCACA 
CTTGAGGTCC TCTCTTATCA TGCCTCCGCT GCGGAGGAAG AGACGCGGGC GCTCCAGGTA 
ACGGCGGCAG CTACGATCCC CTCCGCTCAG TCTCTCAGAC TGATGGACTA CAGTTTCAGT 
GATTTTGACC TGACAGATGC TGAGACGACC CAGGCAACCA TCCGCATGTT TGTGGACCTC 
AAGCTTGTGC AGAATTTTCA GATAAAGTAC AAGAGTTTGT GTCAGTGGAT CTTAAGTGTC 
AAGAAGAACT ACAGAAAGAA TGTGGTCTAC CACAACTGGA GACATGCCTT CAACACCTCC 
CAGTGCATGT TTGCTGTGCT GAAGTCTGGC CGTGTTCAGA ATAATCTGAG TGATTTGGAG 
ACTTTAGCTT TAATGATTGC AACATTGAGT CATGATTTGG ACCACCGAGG GGTCAACAAC 
TCTTACATAA AAAGAAGTGA CCATCCACTG GCCCAACTGT ACTGCCACTC CACAATGGAG 
CATCACCACT TTGACCAGTG TCTCATGATT CTCAACAGCC CTGGTAATCA GATTCTGAGT 
GGTCTTTCCC TGGATGAGTA CAAGGCGACA CTGAAGATGA TTGAGAAGGC TATTTTGGCC 
ACTGACCTGG CTGTCTACAT GAAAAAAAGA ACAGAATTCT TCGAGCTAGC TAAGAACAGT 
CAGTTTGAAT GGGAAGATGA TTGCCACAGG GATCTATTAA GGTCGATGCT GATGACAGCA 
TGCGATATAT CTGCTATTAC AAAACCATGG CCAGTTCAGA AGAAGATAGC AGAACTGGTT 
GCCACGGAGT TCTTTGAGCA AGGCGATAAG GAGCGGAGAG AGCTAAACAT TGAACCTATT 
GATCTGATGA ACAGGGAGAA ACAGGATAAA ATTCCCAGCA TGCAAGTGTC ATTTATTGAT 
GCCATTTGTA CACAGTTATA TGAGGCTCTG ACTGGGATCT CTGAGTTCTG CTCCCCCCTG 
CTGGAGGGCT GCCGGAAGAA CAGGCAGAAT TGGAAGATTC TTGCTGAACA AGGAGAGGAA 
GCAGCGCTGC TAAATGGAGA ACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166620.1
CDS	97..2703
Translation	MEVYPSDSERMMEAWLDDNLEFAHSYFVRKASREMVNAWFAERVHSIQPSREPSKAVPEPVTAVHSLGPTDVVPSSSPAPSTPTRKISASEFDRPLRPIVVKDAEGSFTFLSDTERVQMPLPPSQRPGSDNGTDEGGGIRDRGNGDRCARLLELVRDVSSHLDLTALCHKIFLHTHELIAADRYSLFLVGEDSSNRKFLVSRLFDVAEGSTLEESSNSGIRLEWNKGIVGYVAATGQPLNIKNAYEDSRFNAEVDQITGYKTQSILCLPIKNHRDEVVGVAQAINKKCGENSTFTEQDEKDFSSYLAFSGIVLHNAQLYETSQLENRRNQVLLDLASLIFEEQQSLEVLLRKTVATILSFMQAQECTVFISDRETANTFASVFHMEYEEIAGSTDFPNRECDVNKINYMYAQYVKNTMETLNITDTTKDQLFSWTSEDPQSTNCQIKSLLCTPIRNGKKDKVIGVCQLVNKMDEASGEVKAFNRNDEQFLEAFAVFCGLGIQNTQMYEAVERAMAKQEVTLEVLSYHASAAEEETRALQVTAAATIPSAQSLRLMDYSFSDFDLTDAETTQATIRMFVDLKLVQNFQIKYKSLCQWILSVKKNYRKNVVYHNWRHAFNTSQCMFAVLKSGRVQNNLSDLETLALMIATLSHDLDHRGVNNSYIKRSDHPLAQLYCHSTMEHHHFDQCLMILNSPGNQILSGLSLDEYKATLKMIEKAILATDLAVYMKKRTEFFELAKNSQFEWEDDCHRDLLRSMLMTACDISAITKPWPVQKKIAELVATEFFEQGDKERRELNIEPIDLMNREKQDKIPSMQVSFIDAICTQLYEALTGISEFCSPLLEGCRKNRQNWKILAEQGEEAALLNGEL

Target ORF information:

RefSeq Version	XM_005166563.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphodiesterase 5A, cGMP-specific, b (pde5ab), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166563.3

ORF Insert Sequence:

ATGGAGGTCT ATCCTTCCGA TAGTGAAAGG ATGATGGAGG CTTGGCTTGA CGACAACCTA 
GAGTTCGCAC ATTCTTACTT TGTACGCAAG GCTTCCCGGG AAATGGTCAA TGCCTGGTTT 
GCTGAGCGTG TACACTCCAT TCAACCATCT AGGGAACCAT CAAAAGCTGT GCCAGAACCT 
GTAACAGCAG TCCATTCTCT TGGTCCAACT GACGTGGTGC CATCTTCAAG TCCAGCTCCA 
TCCACCCCAA CACGAAAGAT CTCTGCATCT GAGTTTGACA GGCCGCTTCG CCCCATAGTC 
GTGAAAGACG CAGAGGGTTC CTTCACTTTC CTGAGTGACA CAGAAAGGGT GCAGATGCCG 
CTTCCTCCTT CCCAGCGGCC AGGCTCAGAC AATGGGACAG ATGAGGGTGG AGGTATTAGA 
GATCGTGGTA ATGGGGACCG ATGTGCTCGT CTGTTGGAGC TTGTACGTGA TGTTTCAAGT 
CATTTGGACC TTACTGCCCT CTGCCACAAG ATCTTTTTGC ATACCCATGA GCTGATAGCT 
GCAGATCGCT ACTCGTTGTT CTTGGTTGGG GAGGACAGTT CAAACAGAAA GTTCTTAGTT 
AGCAGGCTTT TCGATGTAGC AGAAGGATCT ACTCTTGAGG AGTCTTCAAA CAGTGGCATC 
CGCCTCGAAT GGAACAAAGG AATTGTGGGA TACGTTGCAG CCACTGGACA GCCTCTCAAC 
ATCAAGAATG CATATGAGGA TTCACGCTTT AATGCAGAAG TGGACCAAAT TACAGGCTAT 
AAGACACAGA GCATCTTGTG TTTGCCCATC AAAAACCACA GAGATGAGGT TGTTGGTGTA 
GCTCAAGCGA TCAATAAAAA GTGTGGAGAG AACAGCACCT TTACAGAACA GGATGAGAAG 
GACTTTTCTT CGTATCTGGC TTTCTCTGGC ATAGTGCTAC ACAATGCCCA ACTCTATGAG 
ACATCACAGC TAGAGAACAG GCGAAACCAG GTGTTGCTGG ATCTGGCTAG TCTGATCTTT 
GAGGAGCAGC AGTCACTGGA GGTCTTACTG AGGAAAACTG TGGCCACCAT CTTATCTTTC 
ATGCAAGCCC AAGAATGCAC AGTGTTCATT TCTGACAGAG AAACAGCTAA CACATTTGCC 
AGTGTGTTTC ACATGGAATA TGAGGAGATT GCTGGATCCA CAGACTTCCC AAACAGAGAA 
TGTGACGTCA ACAAGATTAA CTACATGTAC GCTCAGTATG TCAAAAACAC TATGGAGACA 
CTGAACATCA CAGATACCAC CAAAGATCAG CTATTTTCAT GGACAAGTGA AGATCCACAG 
TCCACGAACT GTCAAATCAA AAGTCTGCTG TGCACACCAA TAAGGAATGG GAAGAAAGAC 
AAAGTAATAG GCGTCTGCCA GTTAGTCAAT AAGATGGATG AAGCTTCAGG GGAGGTGAAA 
GCCTTTAATA GGAATGATGA GCAGTTTCTA GAGGCCTTTG CAGTCTTCTG TGGCCTGGGC 
ATCCAGAACA CACAGATGTA TGAAGCAGTG GAAAGAGCAA TGGCCAAGCA AGAGGTCACA 
CTTGAGGTCC TCTCTTATCA TGCCTCCGCT GCGGAGGAAG AGACGCGGGC GCTCCAGGTA 
ACGGCGGCAG CTACGATCCC CTCCGCTCAG TCTCTCAGAC TGATGGACTA CAGTTTCAGT 
GATTTTGACC TGACAGATGC TGAGACGACC CAGGCAACCA TCCGCATGTT TGTGGACCTC 
AAGCTTGTGC AGAATTTTCA GATAAAGTAC AAGAGTTTGT GTCAGTGGAT CTTAAGTGTC 
AAGAAGAACT ACAGAAAGAA TGTGGTCTAC CACAACTGGA GACATGCCTT CAACACCTCC 
CAGTGCATGT TTGCTGTGCT GAAGTCTGGC CGTGTTCAGA ATAATCTGAG TGATTTGGAG 
ACTTTAGCTT TAATGATTGC AACATTGAGT CATGATTTGG ACCACCGAGG GGTCAACAAC 
TCTTACATAA AAAGAAGTGA CCATCCACTG GCCCAACTGT ACTGCCACTC CACAATGGAG 
CATCACCACT TTGACCAGTG TCTCATGATT CTCAACAGCC CTGGTAATCA GATTCTGAGT 
GGTCTTTCCC TGGATGAGTA CAAGGCGACA CTGAAGATGA TTGAGAAGGC TATTTTGGCC 
ACTGACCTGG CTGTCTACAT GAAAAAAAGA ACAGAATTCT TCGAGCTAGC TAAGAACAGT 
CAGTTTGAAT GGGAAGATGA TTGCCACAGG GATCTATTAA GGTCGATGCT GATGACAGCA 
TGCGATATAT CTGCTATTAC AAAACCATGG CCAGTTCAGA AGAAGATAGC AGAACTGGTT 
GCCACGGAGT TCTTTGAGCA AGGCGATAAG GAGCGGAGAG AGCTAAACAT TGAACCTATT 
GATCTGATGA ACAGGGAGAA ACAGGATAAA ATTCCCAGCA TGCAAGTGTC ATTTATTGAT 
GCCATTTGTA CACAGTTATA TGAGGCTCTG ACTGGGATCT CTGAGTTCTG CTCCCCCCTG 
CTGGAGGGCT GCCGGAAGAA CAGGCAGAAT TGGAAGATTC TTGCTGAACA AGGAGAGGAA 
GCAGCGCTGC TAAATGGAGA ACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde5ab ( XM_005166563.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166620.1 Danio rerio phosphodiesterase 5A, cGMP-specific, b (pde5ab), transcript variant X1, mRNA.

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CloneID	ODa13495
Clone ID Related Accession (Same CDS sequence)	XM_005166563.3 , XM_005166563.4
Accession Version	XM_005166563.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166563.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTCT ATCCTTCCGA TAGTGAAAGG ATGATGGAGG CTTGGCTTGA CGACAACCTA 
GAGTTCGCAC ATTCTTACTT TGTACGCAAG GCTTCCCGGG AAATGGTCAA TGCCTGGTTT 
GCTGAGCGTG TACACTCCAT TCAACCATCT AGGGAACCAT CAAAAGCTGT GCCAGAACCT 
GTAACAGCAG TCCATTCTCT TGGTCCAACT GACGTGGTGC CATCTTCAAG TCCAGCTCCA 
TCCACCCCAA CACGAAAGAT CTCTGCATCT GAGTTTGACA GGCCGCTTCG CCCCATAGTC 
GTGAAAGACG CAGAGGGTTC CTTCACTTTC CTGAGTGACA CAGAAAGGGT GCAGATGCCG 
CTTCCTCCTT CCCAGCGGCC AGGCTCAGAC AATGGGACAG ATGAGGGTGG AGGTATTAGA 
GATCGTGGTA ATGGGGACCG ATGTGCTCGT CTGTTGGAGC TTGTACGTGA TGTTTCAAGT 
CATTTGGACC TTACTGCCCT CTGCCACAAG ATCTTTTTGC ATACCCATGA GCTGATAGCT 
GCAGATCGCT ACTCGTTGTT CTTGGTTGGG GAGGACAGTT CAAACAGAAA GTTCTTAGTT 
AGCAGGCTTT TCGATGTAGC AGAAGGATCT ACTCTTGAGG AGTCTTCAAA CAGTGGCATC 
CGCCTCGAAT GGAACAAAGG AATTGTGGGA TACGTTGCAG CCACTGGACA GCCTCTCAAC 
ATCAAGAATG CATATGAGGA TTCACGCTTT AATGCAGAAG TGGACCAAAT TACAGGCTAT 
AAGACACAGA GCATCTTGTG TTTGCCCATC AAAAACCACA GAGATGAGGT TGTTGGTGTA 
GCTCAAGCGA TCAATAAAAA GTGTGGAGAG AACAGCACCT TTACAGAACA GGATGAGAAG 
GACTTTTCTT CGTATCTGGC TTTCTCTGGC ATAGTGCTAC ACAATGCCCA ACTCTATGAG 
ACATCACAGC TAGAGAACAG GCGAAACCAG GTGTTGCTGG ATCTGGCTAG TCTGATCTTT 
GAGGAGCAGC AGTCACTGGA GGTCTTACTG AGGAAAACTG TGGCCACCAT CTTATCTTTC 
ATGCAAGCCC AAGAATGCAC AGTGTTCATT TCTGACAGAG AAACAGCTAA CACATTTGCC 
AGTGTGTTTC ACATGGAATA TGAGGAGATT GCTGGATCCA CAGACTTCCC AAACAGAGAA 
TGTGACGTCA ACAAGATTAA CTACATGTAC GCTCAGTATG TCAAAAACAC TATGGAGACA 
CTGAACATCA CAGATACCAC CAAAGATCAG CTATTTTCAT GGACAAGTGA AGATCCACAG 
TCCACGAACT GTCAAATCAA AAGTCTGCTG TGCACACCAA TAAGGAATGG GAAGAAAGAC 
AAAGTAATAG GCGTCTGCCA GTTAGTCAAT AAGATGGATG AAGCTTCAGG GGAGGTGAAA 
GCCTTTAATA GGAATGATGA GCAGTTTCTA GAGGCCTTTG CAGTCTTCTG TGGCCTGGGC 
ATCCAGAACA CACAGATGTA TGAAGCAGTG GAAAGAGCAA TGGCCAAGCA AGAGGTCACA 
CTTGAGGTCC TCTCTTATCA TGCCTCCGCT GCGGAGGAAG AGACGCGGGC GCTCCAGGTA 
ACGGCGGCAG CTACGATCCC CTCCGCTCAG TCTCTCAGAC TGATGGACTA CAGTTTCAGT 
GATTTTGACC TGACAGATGC TGAGACGACC CAGGCAACCA TCCGCATGTT TGTGGACCTC 
AAGCTTGTGC AGAATTTTCA GATAAAGTAC AAGAGTTTGT GTCAGTGGAT CTTAAGTGTC 
AAGAAGAACT ACAGAAAGAA TGTGGTCTAC CACAACTGGA GACATGCCTT CAACACCTCC 
CAGTGCATGT TTGCTGTGCT GAAGTCTGGC CGTGTTCAGA ATAATCTGAG TGATTTGGAG 
ACTTTAGCTT TAATGATTGC AACATTGAGT CATGATTTGG ACCACCGAGG GGTCAACAAC 
TCTTACATAA AAAGAAGTGA CCATCCACTG GCCCAACTGT ACTGCCACTC CACAATGGAG 
CATCACCACT TTGACCAGTG TCTCATGATT CTCAACAGCC CTGGTAATCA GATTCTGAGT 
GGTCTTTCCC TGGATGAGTA CAAGGCGACA CTGAAGATGA TTGAGAAGGC TATTTTGGCC 
ACTGACCTGG CTGTCTACAT GAAAAAAAGA ACAGAATTCT TCGAGCTAGC TAAGAACAGT 
CAGTTTGAAT GGGAAGATGA TTGCCACAGG GATCTATTAA GGTCGATGCT GATGACAGCA 
TGCGATATAT CTGCTATTAC AAAACCATGG CCAGTTCAGA AGAAGATAGC AGAACTGGTT 
GCCACGGAGT TCTTTGAGCA AGGCGATAAG GAGCGGAGAG AGCTAAACAT TGAACCTATT 
GATCTGATGA ACAGGGAGAA ACAGGATAAA ATTCCCAGCA TGCAAGTGTC ATTTATTGAT 
GCCATTTGTA CACAGTTATA TGAGGCTCTG ACTGGGATCT CTGAGTTCTG CTCCCCCCTG 
CTGGAGGGCT GCCGGAAGAA CAGGCAGAAT TGGAAGATTC TTGCTGAACA AGGAGAGGAA 
GCAGCGCTGC TAAATGGAGA ACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166620.1
CDS	112..2718
Translation	MEVYPSDSERMMEAWLDDNLEFAHSYFVRKASREMVNAWFAERVHSIQPSREPSKAVPEPVTAVHSLGPTDVVPSSSPAPSTPTRKISASEFDRPLRPIVVKDAEGSFTFLSDTERVQMPLPPSQRPGSDNGTDEGGGIRDRGNGDRCARLLELVRDVSSHLDLTALCHKIFLHTHELIAADRYSLFLVGEDSSNRKFLVSRLFDVAEGSTLEESSNSGIRLEWNKGIVGYVAATGQPLNIKNAYEDSRFNAEVDQITGYKTQSILCLPIKNHRDEVVGVAQAINKKCGENSTFTEQDEKDFSSYLAFSGIVLHNAQLYETSQLENRRNQVLLDLASLIFEEQQSLEVLLRKTVATILSFMQAQECTVFISDRETANTFASVFHMEYEEIAGSTDFPNRECDVNKINYMYAQYVKNTMETLNITDTTKDQLFSWTSEDPQSTNCQIKSLLCTPIRNGKKDKVIGVCQLVNKMDEASGEVKAFNRNDEQFLEAFAVFCGLGIQNTQMYEAVERAMAKQEVTLEVLSYHASAAEEETRALQVTAAATIPSAQSLRLMDYSFSDFDLTDAETTQATIRMFVDLKLVQNFQIKYKSLCQWILSVKKNYRKNVVYHNWRHAFNTSQCMFAVLKSGRVQNNLSDLETLALMIATLSHDLDHRGVNNSYIKRSDHPLAQLYCHSTMEHHHFDQCLMILNSPGNQILSGLSLDEYKATLKMIEKAILATDLAVYMKKRTEFFELAKNSQFEWEDDCHRDLLRSMLMTACDISAITKPWPVQKKIAELVATEFFEQGDKERRELNIEPIDLMNREKQDKIPSMQVSFIDAICTQLYEALTGISEFCSPLLEGCRKNRQNWKILAEQGEEAALLNGEL

Target ORF information:

RefSeq Version	XM_005166563.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphodiesterase 5A, cGMP-specific, b (pde5ab), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166563.4

ORF Insert Sequence:

ATGGAGGTCT ATCCTTCCGA TAGTGAAAGG ATGATGGAGG CTTGGCTTGA CGACAACCTA 
GAGTTCGCAC ATTCTTACTT TGTACGCAAG GCTTCCCGGG AAATGGTCAA TGCCTGGTTT 
GCTGAGCGTG TACACTCCAT TCAACCATCT AGGGAACCAT CAAAAGCTGT GCCAGAACCT 
GTAACAGCAG TCCATTCTCT TGGTCCAACT GACGTGGTGC CATCTTCAAG TCCAGCTCCA 
TCCACCCCAA CACGAAAGAT CTCTGCATCT GAGTTTGACA GGCCGCTTCG CCCCATAGTC 
GTGAAAGACG CAGAGGGTTC CTTCACTTTC CTGAGTGACA CAGAAAGGGT GCAGATGCCG 
CTTCCTCCTT CCCAGCGGCC AGGCTCAGAC AATGGGACAG ATGAGGGTGG AGGTATTAGA 
GATCGTGGTA ATGGGGACCG ATGTGCTCGT CTGTTGGAGC TTGTACGTGA TGTTTCAAGT 
CATTTGGACC TTACTGCCCT CTGCCACAAG ATCTTTTTGC ATACCCATGA GCTGATAGCT 
GCAGATCGCT ACTCGTTGTT CTTGGTTGGG GAGGACAGTT CAAACAGAAA GTTCTTAGTT 
AGCAGGCTTT TCGATGTAGC AGAAGGATCT ACTCTTGAGG AGTCTTCAAA CAGTGGCATC 
CGCCTCGAAT GGAACAAAGG AATTGTGGGA TACGTTGCAG CCACTGGACA GCCTCTCAAC 
ATCAAGAATG CATATGAGGA TTCACGCTTT AATGCAGAAG TGGACCAAAT TACAGGCTAT 
AAGACACAGA GCATCTTGTG TTTGCCCATC AAAAACCACA GAGATGAGGT TGTTGGTGTA 
GCTCAAGCGA TCAATAAAAA GTGTGGAGAG AACAGCACCT TTACAGAACA GGATGAGAAG 
GACTTTTCTT CGTATCTGGC TTTCTCTGGC ATAGTGCTAC ACAATGCCCA ACTCTATGAG 
ACATCACAGC TAGAGAACAG GCGAAACCAG GTGTTGCTGG ATCTGGCTAG TCTGATCTTT 
GAGGAGCAGC AGTCACTGGA GGTCTTACTG AGGAAAACTG TGGCCACCAT CTTATCTTTC 
ATGCAAGCCC AAGAATGCAC AGTGTTCATT TCTGACAGAG AAACAGCTAA CACATTTGCC 
AGTGTGTTTC ACATGGAATA TGAGGAGATT GCTGGATCCA CAGACTTCCC AAACAGAGAA 
TGTGACGTCA ACAAGATTAA CTACATGTAC GCTCAGTATG TCAAAAACAC TATGGAGACA 
CTGAACATCA CAGATACCAC CAAAGATCAG CTATTTTCAT GGACAAGTGA AGATCCACAG 
TCCACGAACT GTCAAATCAA AAGTCTGCTG TGCACACCAA TAAGGAATGG GAAGAAAGAC 
AAAGTAATAG GCGTCTGCCA GTTAGTCAAT AAGATGGATG AAGCTTCAGG GGAGGTGAAA 
GCCTTTAATA GGAATGATGA GCAGTTTCTA GAGGCCTTTG CAGTCTTCTG TGGCCTGGGC 
ATCCAGAACA CACAGATGTA TGAAGCAGTG GAAAGAGCAA TGGCCAAGCA AGAGGTCACA 
CTTGAGGTCC TCTCTTATCA TGCCTCCGCT GCGGAGGAAG AGACGCGGGC GCTCCAGGTA 
ACGGCGGCAG CTACGATCCC CTCCGCTCAG TCTCTCAGAC TGATGGACTA CAGTTTCAGT 
GATTTTGACC TGACAGATGC TGAGACGACC CAGGCAACCA TCCGCATGTT TGTGGACCTC 
AAGCTTGTGC AGAATTTTCA GATAAAGTAC AAGAGTTTGT GTCAGTGGAT CTTAAGTGTC 
AAGAAGAACT ACAGAAAGAA TGTGGTCTAC CACAACTGGA GACATGCCTT CAACACCTCC 
CAGTGCATGT TTGCTGTGCT GAAGTCTGGC CGTGTTCAGA ATAATCTGAG TGATTTGGAG 
ACTTTAGCTT TAATGATTGC AACATTGAGT CATGATTTGG ACCACCGAGG GGTCAACAAC 
TCTTACATAA AAAGAAGTGA CCATCCACTG GCCCAACTGT ACTGCCACTC CACAATGGAG 
CATCACCACT TTGACCAGTG TCTCATGATT CTCAACAGCC CTGGTAATCA GATTCTGAGT 
GGTCTTTCCC TGGATGAGTA CAAGGCGACA CTGAAGATGA TTGAGAAGGC TATTTTGGCC 
ACTGACCTGG CTGTCTACAT GAAAAAAAGA ACAGAATTCT TCGAGCTAGC TAAGAACAGT 
CAGTTTGAAT GGGAAGATGA TTGCCACAGG GATCTATTAA GGTCGATGCT GATGACAGCA 
TGCGATATAT CTGCTATTAC AAAACCATGG CCAGTTCAGA AGAAGATAGC AGAACTGGTT 
GCCACGGAGT TCTTTGAGCA AGGCGATAAG GAGCGGAGAG AGCTAAACAT TGAACCTATT 
GATCTGATGA ACAGGGAGAA ACAGGATAAA ATTCCCAGCA TGCAAGTGTC ATTTATTGAT 
GCCATTTGTA CACAGTTATA TGAGGCTCTG ACTGGGATCT CTGAGTTCTG CTCCCCCCTG 
CTGGAGGGCT GCCGGAAGAA CAGGCAGAAT TGGAAGATTC TTGCTGAACA AGGAGAGGAA 
GCAGCGCTGC TAAATGGAGA ACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde5ab ( XM_009303486.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301761.1 Danio rerio phosphodiesterase 5A, cGMP-specific, b (pde5ab), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa41835
Clone ID Related Accession (Same CDS sequence)	XM_009303486.2 , XM_009303486.3
Accession Version	XM_009303486.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303486.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTCT ATCCTTCCGA TAGTGAAAGG ATGATGGAGG CTTGGCTTGA CGACAACCTA 
GAGTTCGCAC ATTCTTACTT TGTACGCAAG GCTTCCCGGG AAATGGTCAA TGCCTGGTTT 
GCTGAGCGTG TACACTCCAT TCAACCATCT AGGGAACCAT CAAAAGCTGT GCCAGAACCT 
GTAACAGCAG TCCATTCTCT TGGTCCAACT GACGTGGTGC CATCTTCAAG TCCAGCTCCA 
TCCACCCCAA CACGAAAGAT CTCTGCATCT GAGTTTGACA GGCCGCTTCG CCCCATAGTC 
GTGAAAGACG CAGAGGGTTC CTTCACTTTC CTGAGTGACA CAGAAAGGGT GCAGATGCCG 
CTTCCTCCTT CCCAGCGGCC AGGCTCAGAC AATGGGACAG ATGAGGGTGG AGGTATTAGA 
GATCGTGGTA ATGGGGACCG ATGTGCTCGT CTGTTGGAGC TTGTACGTGA TGTTTCAAGT 
CATTTGGACC TTACTGCCCT CTGCCACAAG ATCTTTTTGC ATACCCATGA GCTGATAGCT 
GCAGATCGCT ACTCGTTGTT CTTGGTTGGG GAGGACAGTT CAAACAGAAA GTTCTTAGTT 
AGCAGGCTTT TCGATGTAGC AGAAGGATCT ACTCTTGAGG AGTCTTCAAA CAGTGGCATC 
CGCCTCGAAT GGAACAAAGG AATTGTGGGA TACGTTGCAG CCACTGGACA GCCTCTCAAC 
ATCAAGAATG CATATGAGGA TTCACGCTTT AATGCAGAAG TGGACCAAAT TACAGGCTAT 
AAGACACAGA GCATCTTGTG TTTGCCCATC AAAAACCACA GAGATGAGGT TGTTGGTGTA 
GCTCAAGCGA TCAATAAAAA GTGTGGAGAG AACAGCACCT TTACAGAACA GGATGAGAAG 
GACTTTTCTT CGTATCTGGC TTTCTCTGGC ATAGTGCTAC ACAATGCCCA ACTCTATGAG 
ACATCACAGC TAGAGAACAG GCGAAACCAG GTGTTGCTGG ATCTGGCTAG TCTGATCTTT 
GAGGAGCAGC AGTCACTGGA GGTCTTACTG AGGAAAACTG TGGCCACCAT CTTATCTTTC 
ATGCAAGCCC AAGAATGCAC AGTGTTCATT TCTGACAGAG AAACAGCTAA CACATTTGCC 
AGTGTGTTTC ACATGGAATA TGAGGAGATT GCTGGATCCA CAGACTTCCC AAACAGAGAA 
TGTGACGTCA ACAAGATTAA CTACATGTAC GCTCAGTATG TCAAAAACAC TATGGAGACA 
CTGAACATCA CAGATACCAC CAAAGATCAG CTATTTTCAT GGACAAGTGA AGATCCACAG 
TCCACGAACT GTCAAATCAA AAGTCTGCTG TGCACACCAA TAAGGAATGG GAAGAAAGAC 
AAAGTAATAG GCGTCTGCCA GTTAGTCAAT AAGATGGATG AAGCTTCAGG GGAGGTGAAA 
GCCTTTAATA GGAATGATGA GCAGTTTCTA GAGGCCTTTG CAGTCTTCTG TGGCCTGGGC 
ATCCAGAACA CACAGATGTA TGAAGCAGTG GAAAGAGCAA TGGCCAAGCA AGAGGTCACA 
CTTGAGGTCC TCTCTTATCA TGCCTCCGCT GCGGAGGAAG AGACGCGGGC GCTCCAGGCA 
GCTACGATCC CCTCCGCTCA GTCTCTCAGA CTGATGGACT ACAGTTTCAG TGATTTTGAC 
CTGACAGATG CTGAGACGAC CCAGGCAACC ATCCGCATGT TTGTGGACCT CAAGCTTGTG 
CAGAATTTTC AGATAAAGTA CAAGAGTTTG TGTCAGTGGA TCTTAAGTGT CAAGAAGAAC 
TACAGAAAGA ATGTGGTCTA CCACAACTGG AGACATGCCT TCAACACCTC CCAGTGCATG 
TTTGCTGTGC TGAAGTCTGG CCGTGTTCAG AATAATCTGA GTGATTTGGA GACTTTAGCT 
TTAATGATTG CAACATTGAG TCATGATTTG GACCACCGAG GGGTCAACAA CTCTTACATA 
AAAAGAAGTG ACCATCCACT GGCCCAACTG TACTGCCACT CCACAATGGA GCATCACCAC 
TTTGACCAGT GTCTCATGAT TCTCAACAGC CCTGGTAATC AGATTCTGAG TGGTCTTTCC 
CTGGATGAGT ACAAGGCGAC ACTGAAGATG ATTGAGAAGG CTATTTTGGC CACTGACCTG 
GCTGTCTACA TGAAAAAAAG AACAGAATTC TTCGAGCTAG CTAAGAACAG TCAGTTTGAA 
TGGGAAGATG ATTGCCACAG GGATCTATTA AGGTCGATGC TGATGACAGC ATGCGATATA 
TCTGCTATTA CAAAACCATG GCCAGTTCAG AAGAAGATAG CAGAACTGGT TGCCACGGAG 
TTCTTTGAGC AAGGCGATAA GGAGCGGAGA GAGCTAAACA TTGAACCTAT TGATCTGATG 
AACAGGGAGA AACAGGATAA AATTCCCAGC ATGCAAGTGT CATTTATTGA TGCCATTTGT 
ACACAGTTAT ATGAGGCTCT GACTGGGATC TCTGAGTTCT GCTCCCCCCT GCTGGAGGGC 
TGCCGGAAGA ACAGGCAGAA TTGGAAGATT CTTGCTGAAC AAGGAGAGGA AGCAGCGCTG 
CTAAATGGAG AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301761.1
CDS	97..2694
Translation	MEVYPSDSERMMEAWLDDNLEFAHSYFVRKASREMVNAWFAERVHSIQPSREPSKAVPEPVTAVHSLGPTDVVPSSSPAPSTPTRKISASEFDRPLRPIVVKDAEGSFTFLSDTERVQMPLPPSQRPGSDNGTDEGGGIRDRGNGDRCARLLELVRDVSSHLDLTALCHKIFLHTHELIAADRYSLFLVGEDSSNRKFLVSRLFDVAEGSTLEESSNSGIRLEWNKGIVGYVAATGQPLNIKNAYEDSRFNAEVDQITGYKTQSILCLPIKNHRDEVVGVAQAINKKCGENSTFTEQDEKDFSSYLAFSGIVLHNAQLYETSQLENRRNQVLLDLASLIFEEQQSLEVLLRKTVATILSFMQAQECTVFISDRETANTFASVFHMEYEEIAGSTDFPNRECDVNKINYMYAQYVKNTMETLNITDTTKDQLFSWTSEDPQSTNCQIKSLLCTPIRNGKKDKVIGVCQLVNKMDEASGEVKAFNRNDEQFLEAFAVFCGLGIQNTQMYEAVERAMAKQEVTLEVLSYHASAAEEETRALQAATIPSAQSLRLMDYSFSDFDLTDAETTQATIRMFVDLKLVQNFQIKYKSLCQWILSVKKNYRKNVVYHNWRHAFNTSQCMFAVLKSGRVQNNLSDLETLALMIATLSHDLDHRGVNNSYIKRSDHPLAQLYCHSTMEHHHFDQCLMILNSPGNQILSGLSLDEYKATLKMIEKAILATDLAVYMKKRTEFFELAKNSQFEWEDDCHRDLLRSMLMTACDISAITKPWPVQKKIAELVATEFFEQGDKERRELNIEPIDLMNREKQDKIPSMQVSFIDAICTQLYEALTGISEFCSPLLEGCRKNRQNWKILAEQGEEAALLNGEL

Target ORF information:

RefSeq Version	XM_009303486.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphodiesterase 5A, cGMP-specific, b (pde5ab), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303486.2

ORF Insert Sequence:

ATGGAGGTCT ATCCTTCCGA TAGTGAAAGG ATGATGGAGG CTTGGCTTGA CGACAACCTA 
GAGTTCGCAC ATTCTTACTT TGTACGCAAG GCTTCCCGGG AAATGGTCAA TGCCTGGTTT 
GCTGAGCGTG TACACTCCAT TCAACCATCT AGGGAACCAT CAAAAGCTGT GCCAGAACCT 
GTAACAGCAG TCCATTCTCT TGGTCCAACT GACGTGGTGC CATCTTCAAG TCCAGCTCCA 
TCCACCCCAA CACGAAAGAT CTCTGCATCT GAGTTTGACA GGCCGCTTCG CCCCATAGTC 
GTGAAAGACG CAGAGGGTTC CTTCACTTTC CTGAGTGACA CAGAAAGGGT GCAGATGCCG 
CTTCCTCCTT CCCAGCGGCC AGGCTCAGAC AATGGGACAG ATGAGGGTGG AGGTATTAGA 
GATCGTGGTA ATGGGGACCG ATGTGCTCGT CTGTTGGAGC TTGTACGTGA TGTTTCAAGT 
CATTTGGACC TTACTGCCCT CTGCCACAAG ATCTTTTTGC ATACCCATGA GCTGATAGCT 
GCAGATCGCT ACTCGTTGTT CTTGGTTGGG GAGGACAGTT CAAACAGAAA GTTCTTAGTT 
AGCAGGCTTT TCGATGTAGC AGAAGGATCT ACTCTTGAGG AGTCTTCAAA CAGTGGCATC 
CGCCTCGAAT GGAACAAAGG AATTGTGGGA TACGTTGCAG CCACTGGACA GCCTCTCAAC 
ATCAAGAATG CATATGAGGA TTCACGCTTT AATGCAGAAG TGGACCAAAT TACAGGCTAT 
AAGACACAGA GCATCTTGTG TTTGCCCATC AAAAACCACA GAGATGAGGT TGTTGGTGTA 
GCTCAAGCGA TCAATAAAAA GTGTGGAGAG AACAGCACCT TTACAGAACA GGATGAGAAG 
GACTTTTCTT CGTATCTGGC TTTCTCTGGC ATAGTGCTAC ACAATGCCCA ACTCTATGAG 
ACATCACAGC TAGAGAACAG GCGAAACCAG GTGTTGCTGG ATCTGGCTAG TCTGATCTTT 
GAGGAGCAGC AGTCACTGGA GGTCTTACTG AGGAAAACTG TGGCCACCAT CTTATCTTTC 
ATGCAAGCCC AAGAATGCAC AGTGTTCATT TCTGACAGAG AAACAGCTAA CACATTTGCC 
AGTGTGTTTC ACATGGAATA TGAGGAGATT GCTGGATCCA CAGACTTCCC AAACAGAGAA 
TGTGACGTCA ACAAGATTAA CTACATGTAC GCTCAGTATG TCAAAAACAC TATGGAGACA 
CTGAACATCA CAGATACCAC CAAAGATCAG CTATTTTCAT GGACAAGTGA AGATCCACAG 
TCCACGAACT GTCAAATCAA AAGTCTGCTG TGCACACCAA TAAGGAATGG GAAGAAAGAC 
AAAGTAATAG GCGTCTGCCA GTTAGTCAAT AAGATGGATG AAGCTTCAGG GGAGGTGAAA 
GCCTTTAATA GGAATGATGA GCAGTTTCTA GAGGCCTTTG CAGTCTTCTG TGGCCTGGGC 
ATCCAGAACA CACAGATGTA TGAAGCAGTG GAAAGAGCAA TGGCCAAGCA AGAGGTCACA 
CTTGAGGTCC TCTCTTATCA TGCCTCCGCT GCGGAGGAAG AGACGCGGGC GCTCCAGGCA 
GCTACGATCC CCTCCGCTCA GTCTCTCAGA CTGATGGACT ACAGTTTCAG TGATTTTGAC 
CTGACAGATG CTGAGACGAC CCAGGCAACC ATCCGCATGT TTGTGGACCT CAAGCTTGTG 
CAGAATTTTC AGATAAAGTA CAAGAGTTTG TGTCAGTGGA TCTTAAGTGT CAAGAAGAAC 
TACAGAAAGA ATGTGGTCTA CCACAACTGG AGACATGCCT TCAACACCTC CCAGTGCATG 
TTTGCTGTGC TGAAGTCTGG CCGTGTTCAG AATAATCTGA GTGATTTGGA GACTTTAGCT 
TTAATGATTG CAACATTGAG TCATGATTTG GACCACCGAG GGGTCAACAA CTCTTACATA 
AAAAGAAGTG ACCATCCACT GGCCCAACTG TACTGCCACT CCACAATGGA GCATCACCAC 
TTTGACCAGT GTCTCATGAT TCTCAACAGC CCTGGTAATC AGATTCTGAG TGGTCTTTCC 
CTGGATGAGT ACAAGGCGAC ACTGAAGATG ATTGAGAAGG CTATTTTGGC CACTGACCTG 
GCTGTCTACA TGAAAAAAAG AACAGAATTC TTCGAGCTAG CTAAGAACAG TCAGTTTGAA 
TGGGAAGATG ATTGCCACAG GGATCTATTA AGGTCGATGC TGATGACAGC ATGCGATATA 
TCTGCTATTA CAAAACCATG GCCAGTTCAG AAGAAGATAG CAGAACTGGT TGCCACGGAG 
TTCTTTGAGC AAGGCGATAA GGAGCGGAGA GAGCTAAACA TTGAACCTAT TGATCTGATG 
AACAGGGAGA AACAGGATAA AATTCCCAGC ATGCAAGTGT CATTTATTGA TGCCATTTGT 
ACACAGTTAT ATGAGGCTCT GACTGGGATC TCTGAGTTCT GCTCCCCCCT GCTGGAGGGC 
TGCCGGAAGA ACAGGCAGAA TTGGAAGATT CTTGCTGAAC AAGGAGAGGA AGCAGCGCTG 
CTAAATGGAG AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde5ab ( XM_009303486.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301761.1 Danio rerio phosphodiesterase 5A, cGMP-specific, b (pde5ab), transcript variant X2, mRNA.

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CloneID	ODa41835
Clone ID Related Accession (Same CDS sequence)	XM_009303486.2 , XM_009303486.3
Accession Version	XM_009303486.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303486.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTCT ATCCTTCCGA TAGTGAAAGG ATGATGGAGG CTTGGCTTGA CGACAACCTA 
GAGTTCGCAC ATTCTTACTT TGTACGCAAG GCTTCCCGGG AAATGGTCAA TGCCTGGTTT 
GCTGAGCGTG TACACTCCAT TCAACCATCT AGGGAACCAT CAAAAGCTGT GCCAGAACCT 
GTAACAGCAG TCCATTCTCT TGGTCCAACT GACGTGGTGC CATCTTCAAG TCCAGCTCCA 
TCCACCCCAA CACGAAAGAT CTCTGCATCT GAGTTTGACA GGCCGCTTCG CCCCATAGTC 
GTGAAAGACG CAGAGGGTTC CTTCACTTTC CTGAGTGACA CAGAAAGGGT GCAGATGCCG 
CTTCCTCCTT CCCAGCGGCC AGGCTCAGAC AATGGGACAG ATGAGGGTGG AGGTATTAGA 
GATCGTGGTA ATGGGGACCG ATGTGCTCGT CTGTTGGAGC TTGTACGTGA TGTTTCAAGT 
CATTTGGACC TTACTGCCCT CTGCCACAAG ATCTTTTTGC ATACCCATGA GCTGATAGCT 
GCAGATCGCT ACTCGTTGTT CTTGGTTGGG GAGGACAGTT CAAACAGAAA GTTCTTAGTT 
AGCAGGCTTT TCGATGTAGC AGAAGGATCT ACTCTTGAGG AGTCTTCAAA CAGTGGCATC 
CGCCTCGAAT GGAACAAAGG AATTGTGGGA TACGTTGCAG CCACTGGACA GCCTCTCAAC 
ATCAAGAATG CATATGAGGA TTCACGCTTT AATGCAGAAG TGGACCAAAT TACAGGCTAT 
AAGACACAGA GCATCTTGTG TTTGCCCATC AAAAACCACA GAGATGAGGT TGTTGGTGTA 
GCTCAAGCGA TCAATAAAAA GTGTGGAGAG AACAGCACCT TTACAGAACA GGATGAGAAG 
GACTTTTCTT CGTATCTGGC TTTCTCTGGC ATAGTGCTAC ACAATGCCCA ACTCTATGAG 
ACATCACAGC TAGAGAACAG GCGAAACCAG GTGTTGCTGG ATCTGGCTAG TCTGATCTTT 
GAGGAGCAGC AGTCACTGGA GGTCTTACTG AGGAAAACTG TGGCCACCAT CTTATCTTTC 
ATGCAAGCCC AAGAATGCAC AGTGTTCATT TCTGACAGAG AAACAGCTAA CACATTTGCC 
AGTGTGTTTC ACATGGAATA TGAGGAGATT GCTGGATCCA CAGACTTCCC AAACAGAGAA 
TGTGACGTCA ACAAGATTAA CTACATGTAC GCTCAGTATG TCAAAAACAC TATGGAGACA 
CTGAACATCA CAGATACCAC CAAAGATCAG CTATTTTCAT GGACAAGTGA AGATCCACAG 
TCCACGAACT GTCAAATCAA AAGTCTGCTG TGCACACCAA TAAGGAATGG GAAGAAAGAC 
AAAGTAATAG GCGTCTGCCA GTTAGTCAAT AAGATGGATG AAGCTTCAGG GGAGGTGAAA 
GCCTTTAATA GGAATGATGA GCAGTTTCTA GAGGCCTTTG CAGTCTTCTG TGGCCTGGGC 
ATCCAGAACA CACAGATGTA TGAAGCAGTG GAAAGAGCAA TGGCCAAGCA AGAGGTCACA 
CTTGAGGTCC TCTCTTATCA TGCCTCCGCT GCGGAGGAAG AGACGCGGGC GCTCCAGGCA 
GCTACGATCC CCTCCGCTCA GTCTCTCAGA CTGATGGACT ACAGTTTCAG TGATTTTGAC 
CTGACAGATG CTGAGACGAC CCAGGCAACC ATCCGCATGT TTGTGGACCT CAAGCTTGTG 
CAGAATTTTC AGATAAAGTA CAAGAGTTTG TGTCAGTGGA TCTTAAGTGT CAAGAAGAAC 
TACAGAAAGA ATGTGGTCTA CCACAACTGG AGACATGCCT TCAACACCTC CCAGTGCATG 
TTTGCTGTGC TGAAGTCTGG CCGTGTTCAG AATAATCTGA GTGATTTGGA GACTTTAGCT 
TTAATGATTG CAACATTGAG TCATGATTTG GACCACCGAG GGGTCAACAA CTCTTACATA 
AAAAGAAGTG ACCATCCACT GGCCCAACTG TACTGCCACT CCACAATGGA GCATCACCAC 
TTTGACCAGT GTCTCATGAT TCTCAACAGC CCTGGTAATC AGATTCTGAG TGGTCTTTCC 
CTGGATGAGT ACAAGGCGAC ACTGAAGATG ATTGAGAAGG CTATTTTGGC CACTGACCTG 
GCTGTCTACA TGAAAAAAAG AACAGAATTC TTCGAGCTAG CTAAGAACAG TCAGTTTGAA 
TGGGAAGATG ATTGCCACAG GGATCTATTA AGGTCGATGC TGATGACAGC ATGCGATATA 
TCTGCTATTA CAAAACCATG GCCAGTTCAG AAGAAGATAG CAGAACTGGT TGCCACGGAG 
TTCTTTGAGC AAGGCGATAA GGAGCGGAGA GAGCTAAACA TTGAACCTAT TGATCTGATG 
AACAGGGAGA AACAGGATAA AATTCCCAGC ATGCAAGTGT CATTTATTGA TGCCATTTGT 
ACACAGTTAT ATGAGGCTCT GACTGGGATC TCTGAGTTCT GCTCCCCCCT GCTGGAGGGC 
TGCCGGAAGA ACAGGCAGAA TTGGAAGATT CTTGCTGAAC AAGGAGAGGA AGCAGCGCTG 
CTAAATGGAG AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301761.1
CDS	64..2661
Translation	MEVYPSDSERMMEAWLDDNLEFAHSYFVRKASREMVNAWFAERVHSIQPSREPSKAVPEPVTAVHSLGPTDVVPSSSPAPSTPTRKISASEFDRPLRPIVVKDAEGSFTFLSDTERVQMPLPPSQRPGSDNGTDEGGGIRDRGNGDRCARLLELVRDVSSHLDLTALCHKIFLHTHELIAADRYSLFLVGEDSSNRKFLVSRLFDVAEGSTLEESSNSGIRLEWNKGIVGYVAATGQPLNIKNAYEDSRFNAEVDQITGYKTQSILCLPIKNHRDEVVGVAQAINKKCGENSTFTEQDEKDFSSYLAFSGIVLHNAQLYETSQLENRRNQVLLDLASLIFEEQQSLEVLLRKTVATILSFMQAQECTVFISDRETANTFASVFHMEYEEIAGSTDFPNRECDVNKINYMYAQYVKNTMETLNITDTTKDQLFSWTSEDPQSTNCQIKSLLCTPIRNGKKDKVIGVCQLVNKMDEASGEVKAFNRNDEQFLEAFAVFCGLGIQNTQMYEAVERAMAKQEVTLEVLSYHASAAEEETRALQAATIPSAQSLRLMDYSFSDFDLTDAETTQATIRMFVDLKLVQNFQIKYKSLCQWILSVKKNYRKNVVYHNWRHAFNTSQCMFAVLKSGRVQNNLSDLETLALMIATLSHDLDHRGVNNSYIKRSDHPLAQLYCHSTMEHHHFDQCLMILNSPGNQILSGLSLDEYKATLKMIEKAILATDLAVYMKKRTEFFELAKNSQFEWEDDCHRDLLRSMLMTACDISAITKPWPVQKKIAELVATEFFEQGDKERRELNIEPIDLMNREKQDKIPSMQVSFIDAICTQLYEALTGISEFCSPLLEGCRKNRQNWKILAEQGEEAALLNGEL

Target ORF information:

RefSeq Version	XM_009303486.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphodiesterase 5A, cGMP-specific, b (pde5ab), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303486.3

ORF Insert Sequence:

ATGGAGGTCT ATCCTTCCGA TAGTGAAAGG ATGATGGAGG CTTGGCTTGA CGACAACCTA 
GAGTTCGCAC ATTCTTACTT TGTACGCAAG GCTTCCCGGG AAATGGTCAA TGCCTGGTTT 
GCTGAGCGTG TACACTCCAT TCAACCATCT AGGGAACCAT CAAAAGCTGT GCCAGAACCT 
GTAACAGCAG TCCATTCTCT TGGTCCAACT GACGTGGTGC CATCTTCAAG TCCAGCTCCA 
TCCACCCCAA CACGAAAGAT CTCTGCATCT GAGTTTGACA GGCCGCTTCG CCCCATAGTC 
GTGAAAGACG CAGAGGGTTC CTTCACTTTC CTGAGTGACA CAGAAAGGGT GCAGATGCCG 
CTTCCTCCTT CCCAGCGGCC AGGCTCAGAC AATGGGACAG ATGAGGGTGG AGGTATTAGA 
GATCGTGGTA ATGGGGACCG ATGTGCTCGT CTGTTGGAGC TTGTACGTGA TGTTTCAAGT 
CATTTGGACC TTACTGCCCT CTGCCACAAG ATCTTTTTGC ATACCCATGA GCTGATAGCT 
GCAGATCGCT ACTCGTTGTT CTTGGTTGGG GAGGACAGTT CAAACAGAAA GTTCTTAGTT 
AGCAGGCTTT TCGATGTAGC AGAAGGATCT ACTCTTGAGG AGTCTTCAAA CAGTGGCATC 
CGCCTCGAAT GGAACAAAGG AATTGTGGGA TACGTTGCAG CCACTGGACA GCCTCTCAAC 
ATCAAGAATG CATATGAGGA TTCACGCTTT AATGCAGAAG TGGACCAAAT TACAGGCTAT 
AAGACACAGA GCATCTTGTG TTTGCCCATC AAAAACCACA GAGATGAGGT TGTTGGTGTA 
GCTCAAGCGA TCAATAAAAA GTGTGGAGAG AACAGCACCT TTACAGAACA GGATGAGAAG 
GACTTTTCTT CGTATCTGGC TTTCTCTGGC ATAGTGCTAC ACAATGCCCA ACTCTATGAG 
ACATCACAGC TAGAGAACAG GCGAAACCAG GTGTTGCTGG ATCTGGCTAG TCTGATCTTT 
GAGGAGCAGC AGTCACTGGA GGTCTTACTG AGGAAAACTG TGGCCACCAT CTTATCTTTC 
ATGCAAGCCC AAGAATGCAC AGTGTTCATT TCTGACAGAG AAACAGCTAA CACATTTGCC 
AGTGTGTTTC ACATGGAATA TGAGGAGATT GCTGGATCCA CAGACTTCCC AAACAGAGAA 
TGTGACGTCA ACAAGATTAA CTACATGTAC GCTCAGTATG TCAAAAACAC TATGGAGACA 
CTGAACATCA CAGATACCAC CAAAGATCAG CTATTTTCAT GGACAAGTGA AGATCCACAG 
TCCACGAACT GTCAAATCAA AAGTCTGCTG TGCACACCAA TAAGGAATGG GAAGAAAGAC 
AAAGTAATAG GCGTCTGCCA GTTAGTCAAT AAGATGGATG AAGCTTCAGG GGAGGTGAAA 
GCCTTTAATA GGAATGATGA GCAGTTTCTA GAGGCCTTTG CAGTCTTCTG TGGCCTGGGC 
ATCCAGAACA CACAGATGTA TGAAGCAGTG GAAAGAGCAA TGGCCAAGCA AGAGGTCACA 
CTTGAGGTCC TCTCTTATCA TGCCTCCGCT GCGGAGGAAG AGACGCGGGC GCTCCAGGCA 
GCTACGATCC CCTCCGCTCA GTCTCTCAGA CTGATGGACT ACAGTTTCAG TGATTTTGAC 
CTGACAGATG CTGAGACGAC CCAGGCAACC ATCCGCATGT TTGTGGACCT CAAGCTTGTG 
CAGAATTTTC AGATAAAGTA CAAGAGTTTG TGTCAGTGGA TCTTAAGTGT CAAGAAGAAC 
TACAGAAAGA ATGTGGTCTA CCACAACTGG AGACATGCCT TCAACACCTC CCAGTGCATG 
TTTGCTGTGC TGAAGTCTGG CCGTGTTCAG AATAATCTGA GTGATTTGGA GACTTTAGCT 
TTAATGATTG CAACATTGAG TCATGATTTG GACCACCGAG GGGTCAACAA CTCTTACATA 
AAAAGAAGTG ACCATCCACT GGCCCAACTG TACTGCCACT CCACAATGGA GCATCACCAC 
TTTGACCAGT GTCTCATGAT TCTCAACAGC CCTGGTAATC AGATTCTGAG TGGTCTTTCC 
CTGGATGAGT ACAAGGCGAC ACTGAAGATG ATTGAGAAGG CTATTTTGGC CACTGACCTG 
GCTGTCTACA TGAAAAAAAG AACAGAATTC TTCGAGCTAG CTAAGAACAG TCAGTTTGAA 
TGGGAAGATG ATTGCCACAG GGATCTATTA AGGTCGATGC TGATGACAGC ATGCGATATA 
TCTGCTATTA CAAAACCATG GCCAGTTCAG AAGAAGATAG CAGAACTGGT TGCCACGGAG 
TTCTTTGAGC AAGGCGATAA GGAGCGGAGA GAGCTAAACA TTGAACCTAT TGATCTGATG 
AACAGGGAGA AACAGGATAA AATTCCCAGC ATGCAAGTGT CATTTATTGA TGCCATTTGT 
ACACAGTTAT ATGAGGCTCT GACTGGGATC TCTGAGTTCT GCTCCCCCCT GCTGGAGGGC 
TGCCGGAAGA ACAGGCAGAA TTGGAAGATT CTTGCTGAAC AAGGAGAGGA AGCAGCGCTG 
CTAAATGGAG AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Identification of amino acid residues responsible for the selectivity of tadalafil binding to two closely related phosphodiesterases, PDE5 and PDE6.
The Journal of biological chemistry287(49)41406-16(2012 Nov)
Cahill KB,Quade JH,Carleton KL,Cote RH

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pde5ab cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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