gRNAs
The following Pde5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa01007 | |
| Clone ID Related Accession (Same CDS sequence) | NM_133584.1 | |
| Accession Version | NM_133584.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-22 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | cGMP-specific 3',5'-cyclic phosphodiesterase | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D89093.1. On or before Jun 25, 2006 this sequence version replaced XM_001074236.1, XM_001074257.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D89093.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGTTGCCCT TTGGAGACAA AACGAGAGAC ATGGTCAATG CATGGTTTTC AGAGAGAGTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_598268.1 |
| CDS | 475..2976 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_133584.1 |
1 | ATGTTGCCCT TTGGAGACAA AACGAGAGAC ATGGTCAATG CATGGTTTTC AGAGAGAGTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa25233 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006233260.3 | |
| Accession Version | XM_006233260.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233260.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAACGAG CGGGCCCCAG CTCCGTGCAG TCGCAGCAGC AGCGGGACCA GGACTGGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006233322.1 |
| CDS | 112..2709 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006233260.3 |
1 | ATGGAACGAG CGGGCCCCAG CTCCGTGCAG TCGCAGCAGC AGCGGGACCA GGACTGGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Type of supplemented simple sugar, not merely calorie intake, determines adverse effects on metabolism and aortic function in female rats. |
Upregulation of Phosphodiesterase type 5 in the Hyperplastic Prostate. |
Chronic vasodilation increases renal medullary PDE5A and ?-ENaC through independent renin-angiotensin-aldosterone system pathways. |
cGMP-Prkg1 signaling and Pde5 inhibition shelter cochlear hair cells and hearing function. |
Phosphodiesterase 5A inhibition decreases NHE-1 activity without altering steady state pH(i): role of phosphatases. |
