gRNAs
The following PDE5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OBa41342 | |
| Clone ID Related Accession (Same CDS sequence) | NM_174417.2 | |
| Accession Version | NM_174417.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-19 | |
| Organism | Bos taurus(cattle) | |
| Product | cGMP-specific 3',5'-cyclic phosphodiesterase | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L16545.1. On or before Mar 29, 2005 this sequence version replaced XM_602967.1, NM_174417.1. ##Evidence-Data-START## Transcript exon combination :: L16545.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145424 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGAGAGGG CCGGCCCCGG CTCCGCGCGG CCGCAACAGC AATGGGACCA GGACTCGGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_776842.1 |
| CDS | 99..2696 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_174417.2 |
1 | ATGGAGAGGG CCGGCCCCGG CTCCGCGCGG CCGCAACAGC AATGGGACCA GGACTCGGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa235118 | |
| Clone ID Related Accession (Same CDS sequence) | XM_024993001.1 | |
| Accession Version | XM_024993001.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTTGCCTT TTGGAGACAA AACAAGAGAA ATGGTCAACG CATGGTTTGC TGAGAGAGTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_024848769.1 |
| CDS | 162..2663 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_024993001.1 |
1 | ATGTTGCCTT TTGGAGACAA AACAAGAGAA ATGGTCAACG CATGGTTTGC TGAGAGAGTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa235119 | |
| Clone ID Related Accession (Same CDS sequence) | XM_024993002.1 | |
| Accession Version | XM_024993002.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGTCAACG CATGGTTTGC TGAGAGAGTT CACACCATTC CTGTGTGCAA GGAAGGAATC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_024848770.1 |
| CDS | 275..2746 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_024993002.1 |
1 | ATGGTCAACG CATGGTTTGC TGAGAGAGTT CACACCATTC CTGTGTGCAA GGAAGGAATC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
A whole-genome assembly of the domestic cow, Bos taurus. |
Ectopic expression of bovine type 5 phosphodiesterase confers a renal phenotype in Drosophila. |
Binding of cGMP to both allosteric sites of cGMP-binding cGMP-specific phosphodiesterase (PDE5) is required for its phosphorylation. |
Identification of key amino acids in a conserved cGMP-binding site of cGMP-binding phosphodiesterases. A putative NKXnD motif for cGMP binding. |
An essential aspartic acid at each of two allosteric cGMP-binding sites of a cGMP-specific phosphodiesterase. |
