pde5a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following pde5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pde5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa24056 NM_001079344.1
Latest version!
Xenopus tropicalis phosphodiesterase 5A (pde5a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa29651 XM_012966026.2
Latest version!
Xenopus tropicalis phosphodiesterase 5A (pde5a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa29652 XM_012966035.2
Latest version!
Xenopus tropicalis phosphodiesterase 5A (pde5a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa29653 XM_012966039.2
Latest version!
Xenopus tropicalis phosphodiesterase 5A (pde5a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa29654 XM_012966045.2
Latest version!
Xenopus tropicalis phosphodiesterase 5A (pde5a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa48073 XM_012966026.3
Latest version!
Xenopus tropicalis phosphodiesterase 5A (pde5a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa48074 XM_012966035.3
Latest version!
Xenopus tropicalis phosphodiesterase 5A (pde5a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa48075 XM_012966045.3
Latest version!
Xenopus tropicalis phosphodiesterase 5A (pde5a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa48076 XM_031902313.1
Latest version!
Xenopus tropicalis phosphodiesterase 5A (pde5a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa24056
    Clone ID Related Accession (Same CDS sequence) NM_001079344.1
    Accession Version NM_001079344.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Xenopus tropicalis(tropical clawed frog)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC121494.1. ##Evidence-Data-START## Transcript exon combination :: BC121494.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028291, SAMD00028292 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTCAACG CATGGTTTGC TGAACGAGTT CATACAATAC CAGCTAGTAA AGAGCAAGGA 
    AAAAATAATT CAGAGTCTAA TCCTCAGGTG TGCTATTCAG AAAATACCAC TCAGGGAACT
    CCAGTGAGGA AGATCTCAGC CTCAGAGTTC GATAGGCCCC TAAGGCCAAT TGTTGTCAAA
    GATTCTGTCG GAACACTGAA ATTTCTTTCT GATTCTGAAA AGAAGGAACT GATGCCTCTT
    CAACCACCCA GTCTGTCCAG AGCAGGAGAT CAGTGCTCAA GGCTTTTGGA ACTGGTGAAA
    GATATTTCAA GTCACCTTGA CGTCACAACA CTTTGTCATA AAATTTTTTT GCACATCCAT
    GAGCTAATAG CAGCAGATCG TTATTCGCTC TTTTTGGTTT GTGAGGACAG TTCCAATAAG
    AAGTTTCTGG TCAGTAGACT GTTTGACGTG GCAGAAGGTA CAACTGTGGA ACAGGCTTCA
    AACAATTGCA TCCGTTTGGA ATGGAATAAA GGGATTGTTG GTCACGTGGC AGAGTTTGGT
    CAGCCCTTGA ATATAAAGGA TGCTTACCAG GACTCCAGAT TCAATGCGGA AGTCGACCAA
    ATTACTGGGT ACAAAACACA AAGTATTCTT TGTATGCCAA TTAAGAACCA CAGGGAAGAG
    GTGGTTGGTG TAGCACAAGC AATCAATAAG AAGTCAGGAA GCAATGGGAC ATTTACTGAC
    CAAGATGAAA AGGACTTTGC TGCTTATCTG GCATTCTGCG GAATTGTTCT ACACAATGCA
    CAACTTTACG AAACATCACT GCTTGAAAAC AGGAGGAACC AGGTGCTGCT TGACCTTGCA
    ACCTTGATAT TTGAAGAACA GCAATCACTG GAAGTCATCT TAAAGAAGAT CGCAGCTACC
    ATTCTGTCAT TTATGCAGGC TCAGAGATGC ACAATATTCA TTGTGGATGA AGATAGTCCT
    GATACCTTTT CAAGTGTGTT TCATATGGAG TCTGATGAAT TAGAAGAGAC TGCAGATGCT
    GTAAAAAGGG AATATGATGT GAGCAAAATG AATTACATGT ATGCACAGTA TGTAGCAAAT
    ACAATGGAAG CAATTAACAT TCCAGACATA TGTAAGGACA CAAGATTTCC ATGGACAAAC
    GGAAATGAAG AGAAGAATTT CAAGCATATT CAGAGTTTGC TTTGCTCCCC AATCAAGAAT
    GCAAAGAAGA ATAAAGTAAT AGGAGTCTGT CAACTTGTAA ATAAGGTGGA TGAAAACTCT
    GGCAAAATTA AAGCATTCAA CAAAAACGAT GAACAGTTTC TAGAAGCCTT TGCCATATTC
    TGTGGCTTGG GAATTCAGAA CACCCAGATG TTTGAAGTTG TGGAGCGTGC GATGGCCAAA
    CAGATGGTGA CATTAGAGAT TCTTTCATAT CACGCATCTG CTGCTGAGGA AGAAACAATG
    GAGCTGCAGG TAACAGCGGG AGCCATTGTG CCATCTGCAC AATCGCTTAA ACTGACAGAA
    TTCTACTTCA GCGATTTTGA GCTTTCTGAC ATGGAAACCA CATTAGCCAC AAGCCGAATG
    TTCACAGACC TCAACCTTGT GCAGACCTTC CAAATGAAAT ATGAGACCCT TTGCCGGTGG
    ATCCTGAGCG TGAAGAAGAA CTATAGAAAA AATGTTGCAT ATCACAACTG GAGACATGCC
    TTTAATACAG CCCAGTGTAT GTTCGCAGCC CTGAGAACTG GCAAAATTCA GGGTAAGCTA
    AATGACCTAG AAATCCTTGC ACTGATGATA GCCACCTTGA GCCATGACTT GGACCACAGA
    GGAGTTAACA ATTCTTTTAT ACAGAGAAGT GAACATCCTC TAGCACAGCT ATACTGTCAT
    TCTATCATGG AGAACCACCA TTTTGACCAG TGCCTAATGA TACTAAACAG TCAGGGTAAC
    CAGATTCTAA GTGGGCTTTC TGTTCAAGAC TACAAGACTG TTCTGAAGAT GATAAAACAA
    GCCATCCTTG CTACTGATTT GGCACTATAC ATTAAGCGAA GAAGTGATTT CTTTGAATTA
    GTGAATAAGA AGAAATTCAA ATGGGATGAT CCTTCACAAA AAGAACTGTT CTTAGCAATG
    CTGATGACGG CTTGTGATCT GTCTGCAATA ACAAAGCCAT GGCCAGTTCA ACAACGGATA
    GCTGAACTTG TAGCCTCAGA GTTTTATGAC CAAGGGGATA AAGAGAGGAG GGAACTTAAT
    ATTGAACCAA TAGACCTGAT GAACAGAGAA AAGAAAGACA AAATACCCAG CATGCAGGTT
    GGATTTATTG ACGCAGTCTG CTTAGAACTT TATGAGGCCT TAACGCAGAT CTCTGAATCG
    TGTTACCCTT TACTTGATGG CTGTCAGAGA AATAGGCAGA TGTGGCAGAT CTTAGCAGAG
    CAGCAGGAAA AGAACTTGGT GAATGGGGCG AAGAGCCAGC CTGAAGTGAA GTGCAACATG
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001072812.1
    CDS431..2896
    Translation

    Target ORF information:

    RefSeq Version NM_001079344.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphodiesterase 5A (pde5a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079344.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTCAACG CATGGTTTGC TGAACGAGTT CATACAATAC CAGCTAGTAA AGAGCAAGGA 
    AAAAATAATT CAGAGTCTAA TCCTCAGGTG TGCTATTCAG AAAATACCAC TCAGGGAACT
    CCAGTGAGGA AGATCTCAGC CTCAGAGTTC GATAGGCCCC TAAGGCCAAT TGTTGTCAAA
    GATTCTGTCG GAACACTGAA ATTTCTTTCT GATTCTGAAA AGAAGGAACT GATGCCTCTT
    CAACCACCCA GTCTGTCCAG AGCAGGAGAT CAGTGCTCAA GGCTTTTGGA ACTGGTGAAA
    GATATTTCAA GTCACCTTGA CGTCACAACA CTTTGTCATA AAATTTTTTT GCACATCCAT
    GAGCTAATAG CAGCAGATCG TTATTCGCTC TTTTTGGTTT GTGAGGACAG TTCCAATAAG
    AAGTTTCTGG TCAGTAGACT GTTTGACGTG GCAGAAGGTA CAACTGTGGA ACAGGCTTCA
    AACAATTGCA TCCGTTTGGA ATGGAATAAA GGGATTGTTG GTCACGTGGC AGAGTTTGGT
    CAGCCCTTGA ATATAAAGGA TGCTTACCAG GACTCCAGAT TCAATGCGGA AGTCGACCAA
    ATTACTGGGT ACAAAACACA AAGTATTCTT TGTATGCCAA TTAAGAACCA CAGGGAAGAG
    GTGGTTGGTG TAGCACAAGC AATCAATAAG AAGTCAGGAA GCAATGGGAC ATTTACTGAC
    CAAGATGAAA AGGACTTTGC TGCTTATCTG GCATTCTGCG GAATTGTTCT ACACAATGCA
    CAACTTTACG AAACATCACT GCTTGAAAAC AGGAGGAACC AGGTGCTGCT TGACCTTGCA
    ACCTTGATAT TTGAAGAACA GCAATCACTG GAAGTCATCT TAAAGAAGAT CGCAGCTACC
    ATTCTGTCAT TTATGCAGGC TCAGAGATGC ACAATATTCA TTGTGGATGA AGATAGTCCT
    GATACCTTTT CAAGTGTGTT TCATATGGAG TCTGATGAAT TAGAAGAGAC TGCAGATGCT
    GTAAAAAGGG AATATGATGT GAGCAAAATG AATTACATGT ATGCACAGTA TGTAGCAAAT
    ACAATGGAAG CAATTAACAT TCCAGACATA TGTAAGGACA CAAGATTTCC ATGGACAAAC
    GGAAATGAAG AGAAGAATTT CAAGCATATT CAGAGTTTGC TTTGCTCCCC AATCAAGAAT
    GCAAAGAAGA ATAAAGTAAT AGGAGTCTGT CAACTTGTAA ATAAGGTGGA TGAAAACTCT
    GGCAAAATTA AAGCATTCAA CAAAAACGAT GAACAGTTTC TAGAAGCCTT TGCCATATTC
    TGTGGCTTGG GAATTCAGAA CACCCAGATG TTTGAAGTTG TGGAGCGTGC GATGGCCAAA
    CAGATGGTGA CATTAGAGAT TCTTTCATAT CACGCATCTG CTGCTGAGGA AGAAACAATG
    GAGCTGCAGG TAACAGCGGG AGCCATTGTG CCATCTGCAC AATCGCTTAA ACTGACAGAA
    TTCTACTTCA GCGATTTTGA GCTTTCTGAC ATGGAAACCA CATTAGCCAC AAGCCGAATG
    TTCACAGACC TCAACCTTGT GCAGACCTTC CAAATGAAAT ATGAGACCCT TTGCCGGTGG
    ATCCTGAGCG TGAAGAAGAA CTATAGAAAA AATGTTGCAT ATCACAACTG GAGACATGCC
    TTTAATACAG CCCAGTGTAT GTTCGCAGCC CTGAGAACTG GCAAAATTCA GGGTAAGCTA
    AATGACCTAG AAATCCTTGC ACTGATGATA GCCACCTTGA GCCATGACTT GGACCACAGA
    GGAGTTAACA ATTCTTTTAT ACAGAGAAGT GAACATCCTC TAGCACAGCT ATACTGTCAT
    TCTATCATGG AGAACCACCA TTTTGACCAG TGCCTAATGA TACTAAACAG TCAGGGTAAC
    CAGATTCTAA GTGGGCTTTC TGTTCAAGAC TACAAGACTG TTCTGAAGAT GATAAAACAA
    GCCATCCTTG CTACTGATTT GGCACTATAC ATTAAGCGAA GAAGTGATTT CTTTGAATTA
    GTGAATAAGA AGAAATTCAA ATGGGATGAT CCTTCACAAA AAGAACTGTT CTTAGCAATG
    CTGATGACGG CTTGTGATCT GTCTGCAATA ACAAAGCCAT GGCCAGTTCA ACAACGGATA
    GCTGAACTTG TAGCCTCAGA GTTTTATGAC CAAGGGGATA AAGAGAGGAG GGAACTTAAT
    ATTGAACCAA TAGACCTGAT GAACAGAGAA AAGAAAGACA AAATACCCAG CATGCAGGTT
    GGATTTATTG ACGCAGTCTG CTTAGAACTT TATGAGGCCT TAACGCAGAT CTCTGAATCG
    TGTTACCCTT TACTTGATGG CTGTCAGAGA AATAGGCAGA TGTGGCAGAT CTTAGCAGAG
    CAGCAGGAAA AGAACTTGGT GAATGGGGCG AAGAGCCAGC CTGAAGTGAA GTGCAACATG
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29651
    Clone ID Related Accession (Same CDS sequence) XM_012966026.2
    Accession Version XM_012966026.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2832bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966026.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCGCCTCT CACTTCCGTC TGCTGCTGCT GGGGTGGATC CGAAGGGCAA TCACCACACA 
    GATGCATTTA GGAAGTTGTG GAAAACCTCC TCACTCGAGA GCTGCCTTTT ATTTATACAT
    TCTTCTTATG TCCCCCCTCC CTGTGCGCTA TTCTTTCGCC GGTTCCCTTC CCCGGCCTGT
    CTTGGAAAGA GAATGTGGCA GGGGAATCTT TACCGCGAAG CCTCAGTGGC CGCTGTTGGT
    TTCCCTCTGG CTGCTGCCCC GGAGATGGGG GACACCCCCG AACCCCAAGT TGAAAACTGG
    GCTGCTACCC ATTCGGCTAC AGTGAACTCC AGCTGCAATG CGCATGCGTG CTGCACTCTA
    CAAGAAATGG TCAACGCATG GTTTGCTGAA CGAGTTCATA CAATACCAGC TAGTAAAGAG
    CAAGGAAAAA ATAATTCAGA GTCTAATCCT CAGGTGTGCT ATTCAGAAAA TACCACTCAG
    GGAACTCCAG TGAGGAAGAT CTCAGCCTCA GAGTTCGATA GGCCCCTAAG GCCAATTGTT
    GTCAAAGATT CTGTCGGAAC ACTGAAATTT CTTTCTGATT CTGAAAAGAA GGAACTGATG
    CCTCTTCAAC CACCCAGTCT GTCCAGAGCA GGAGATCAGT GCTCAAGGCT TTTGGAACTG
    GTGAAAGATA TTTCAAGTCA CCTTGACGTC ACAACACTTT GTCATAAAAT TTTTTTGCAC
    ATCCATGAGC TAATAGCAGC AGATCGTTAT TCGCTCTTTT TGGTTTGTGA GGACAGTTCC
    AATAAGAAGT TTCTGGTCAG TAGACTGTTT GACGTGGCAG AAGGTACAAC TGTGGAACAG
    GCTTCAAACA ATTGCATCCG TTTGGAATGG AATAAAGGGA TTGTTGGTCA CGTGGCAGAG
    TTTGGTCAGC CCTTGAATAT AAAGGATGCT TACCAGGACT CCAGATTCAA TGCGGAAGTC
    GACCAAATTA CTGGGTACAA AACACAAAGT ATTCTTTGTA TGCCAATTAA GAACCACAGG
    GAAGAGGTGG TTGGTGTAGC ACAAGCAATC AATAAGAAGT CAGGAAGCAA TGGGACATTT
    ACTGACCAAG ATGAAAAGGA CTTTGCTGCT TATCTGGCAT TCTGCGGAAT TGTTCTACAC
    AATGCACAAC TTTACGAAAC ATCACTGCTT GAAAACAGGA GGAACCAGGT GCTGCTTGAC
    CTTGCAACCT TGATATTTGA AGAACAGCAA TCACTGGAAG TCATCTTAAA GAAGATCGCA
    GCTACCATTC TGTCATTTAT GCAGGCTCAG AGATGCACAA TATTCATTGT GGATGAAGAT
    AGTCCTGATA CCTTTTCAAG TGTGTTTCAT ATGGAGTCTG ATGAATTAGA AGAGTCTGCA
    GATGCTGTAA AAAGGGAATA TGATGTGAGC AAAATGAATT ACATGTATGC ACAGTATGTA
    GCAAATACAA TGGAAGCAAT TAACATTCCA GACATATGTA AGGACACAAG ATTTCCATGG
    ACAAACGGAA ATGAAGAGAA GAATTTCAAG CATATTCAGA GTTTGCTTTG CTCCCCAATC
    AAGAATGCAA AGAAGAATAA AGTAATAGGA GTCTGTCAAC TTGTAAATAA GGTGGATGAA
    AACTCTGGCA AAATTAAAGC ATTCAACAAA AACGATGAAC AGTTTCTAGA AGCCTTTGCC
    ATATTCTGTG GCTTGGGAAT TCAGAACACC CAGATGTTTG AAGTTGTGGA GCGTGCGATG
    GCCAAACAGA TGGTGACATT AGAGATTCTT TCATATCACG CATCTGCTGC TGAGGAAGAA
    ACAATGGAGC TGCAGGTAAC AGCGGGAGCC ATTGTGCCAT CTGCACAATC GCTTAAACTG
    ACAGAATTCT ACTTCAGCGA TTTTGAGCTT TCTGACATGG AAACCACATT AGCCACAAGC
    CGAATGTTCA CAGACCTCAA CCTTGTGCAG ACCTTCCAAA TGAAATATGA GACCCTTTGC
    CGGTGGATCC TGAGCGTGAA GAAGAACTAT AGAAAAAATG TTGCATATCA CAACTGGAGA
    CATGCCTTTA ATACAGCCCA GTGTATGTTC GCAGCCCTGA GAACTGGCAA AATTCAGGGT
    AAGCTAAATG ACCTAGAAAT CCTTGCACTG ATGATAGCCA CCTTGAGCCA TGACTTGGAC
    CACAGAGGAG TTAACAATTC TTTTATACAG AGAAGTGAAC ATCCTCTAGC ACAGCTATAC
    TGTCATTCTA TCATGGAGAA CCACCATTTT GACCAGTGCC TAATGATACT AAACAGTCAG
    GGTAACCAGA TTCTAAGTGG GCTTTCTGTT CAAGACTACA AGACTGTTCT GAAGATGATA
    AAACAAGCCA TCCTTGCTAC TGATTTGGCA CTATACATTA AGCGAAGAAG TGATTTCTTT
    GAATTAGTGA ATAAGAAGAA ATTCAAATGG GATGATCCTT CACAAAAAGA ACTGTTCTTA
    GCAATGCTGA TGACGGCTTG TGATCTGTCT GCAATAACAA AGCCATGGCC AGTTCAACAA
    CGGATAGCTG AACTTGTAGC CTCAGAGTTT TATGACCAAG GGGATAAAGA GAGGAGGGAA
    CTTAATATTG AACCAATAGA CCTGATGAAC AGAGAAAAGA AAGACAAAAT ACCCAGCATG
    CAGGTTGGAT TTATTGATGC AGTCTGCTTA GAACTTTATG AGGCCTTAAC GCAGATCTCT
    GAATCGTGTT ACCCTTTACT TGATGGCTGT CAGAGAAATA GGCAGATGTG GCAGATCTTA
    GCAGAGCAGC AGGAAAAGAA CTTGGTGAAT GGGGCGAAGA GCCAGCCTGA AGTGAAGTGC
    AACATGGATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821480.1
    CDS435..3266
    Translation

    Target ORF information:

    RefSeq Version XM_012966026.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphodiesterase 5A (pde5a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966026.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCGCCTCT CACTTCCGTC TGCTGCTGCT GGGGTGGATC CGAAGGGCAA TCACCACACA 
    GATGCATTTA GGAAGTTGTG GAAAACCTCC TCACTCGAGA GCTGCCTTTT ATTTATACAT
    TCTTCTTATG TCCCCCCTCC CTGTGCGCTA TTCTTTCGCC GGTTCCCTTC CCCGGCCTGT
    CTTGGAAAGA GAATGTGGCA GGGGAATCTT TACCGCGAAG CCTCAGTGGC CGCTGTTGGT
    TTCCCTCTGG CTGCTGCCCC GGAGATGGGG GACACCCCCG AACCCCAAGT TGAAAACTGG
    GCTGCTACCC ATTCGGCTAC AGTGAACTCC AGCTGCAATG CGCATGCGTG CTGCACTCTA
    CAAGAAATGG TCAACGCATG GTTTGCTGAA CGAGTTCATA CAATACCAGC TAGTAAAGAG
    CAAGGAAAAA ATAATTCAGA GTCTAATCCT CAGGTGTGCT ATTCAGAAAA TACCACTCAG
    GGAACTCCAG TGAGGAAGAT CTCAGCCTCA GAGTTCGATA GGCCCCTAAG GCCAATTGTT
    GTCAAAGATT CTGTCGGAAC ACTGAAATTT CTTTCTGATT CTGAAAAGAA GGAACTGATG
    CCTCTTCAAC CACCCAGTCT GTCCAGAGCA GGAGATCAGT GCTCAAGGCT TTTGGAACTG
    GTGAAAGATA TTTCAAGTCA CCTTGACGTC ACAACACTTT GTCATAAAAT TTTTTTGCAC
    ATCCATGAGC TAATAGCAGC AGATCGTTAT TCGCTCTTTT TGGTTTGTGA GGACAGTTCC
    AATAAGAAGT TTCTGGTCAG TAGACTGTTT GACGTGGCAG AAGGTACAAC TGTGGAACAG
    GCTTCAAACA ATTGCATCCG TTTGGAATGG AATAAAGGGA TTGTTGGTCA CGTGGCAGAG
    TTTGGTCAGC CCTTGAATAT AAAGGATGCT TACCAGGACT CCAGATTCAA TGCGGAAGTC
    GACCAAATTA CTGGGTACAA AACACAAAGT ATTCTTTGTA TGCCAATTAA GAACCACAGG
    GAAGAGGTGG TTGGTGTAGC ACAAGCAATC AATAAGAAGT CAGGAAGCAA TGGGACATTT
    ACTGACCAAG ATGAAAAGGA CTTTGCTGCT TATCTGGCAT TCTGCGGAAT TGTTCTACAC
    AATGCACAAC TTTACGAAAC ATCACTGCTT GAAAACAGGA GGAACCAGGT GCTGCTTGAC
    CTTGCAACCT TGATATTTGA AGAACAGCAA TCACTGGAAG TCATCTTAAA GAAGATCGCA
    GCTACCATTC TGTCATTTAT GCAGGCTCAG AGATGCACAA TATTCATTGT GGATGAAGAT
    AGTCCTGATA CCTTTTCAAG TGTGTTTCAT ATGGAGTCTG ATGAATTAGA AGAGTCTGCA
    GATGCTGTAA AAAGGGAATA TGATGTGAGC AAAATGAATT ACATGTATGC ACAGTATGTA
    GCAAATACAA TGGAAGCAAT TAACATTCCA GACATATGTA AGGACACAAG ATTTCCATGG
    ACAAACGGAA ATGAAGAGAA GAATTTCAAG CATATTCAGA GTTTGCTTTG CTCCCCAATC
    AAGAATGCAA AGAAGAATAA AGTAATAGGA GTCTGTCAAC TTGTAAATAA GGTGGATGAA
    AACTCTGGCA AAATTAAAGC ATTCAACAAA AACGATGAAC AGTTTCTAGA AGCCTTTGCC
    ATATTCTGTG GCTTGGGAAT TCAGAACACC CAGATGTTTG AAGTTGTGGA GCGTGCGATG
    GCCAAACAGA TGGTGACATT AGAGATTCTT TCATATCACG CATCTGCTGC TGAGGAAGAA
    ACAATGGAGC TGCAGGTAAC AGCGGGAGCC ATTGTGCCAT CTGCACAATC GCTTAAACTG
    ACAGAATTCT ACTTCAGCGA TTTTGAGCTT TCTGACATGG AAACCACATT AGCCACAAGC
    CGAATGTTCA CAGACCTCAA CCTTGTGCAG ACCTTCCAAA TGAAATATGA GACCCTTTGC
    CGGTGGATCC TGAGCGTGAA GAAGAACTAT AGAAAAAATG TTGCATATCA CAACTGGAGA
    CATGCCTTTA ATACAGCCCA GTGTATGTTC GCAGCCCTGA GAACTGGCAA AATTCAGGGT
    AAGCTAAATG ACCTAGAAAT CCTTGCACTG ATGATAGCCA CCTTGAGCCA TGACTTGGAC
    CACAGAGGAG TTAACAATTC TTTTATACAG AGAAGTGAAC ATCCTCTAGC ACAGCTATAC
    TGTCATTCTA TCATGGAGAA CCACCATTTT GACCAGTGCC TAATGATACT AAACAGTCAG
    GGTAACCAGA TTCTAAGTGG GCTTTCTGTT CAAGACTACA AGACTGTTCT GAAGATGATA
    AAACAAGCCA TCCTTGCTAC TGATTTGGCA CTATACATTA AGCGAAGAAG TGATTTCTTT
    GAATTAGTGA ATAAGAAGAA ATTCAAATGG GATGATCCTT CACAAAAAGA ACTGTTCTTA
    GCAATGCTGA TGACGGCTTG TGATCTGTCT GCAATAACAA AGCCATGGCC AGTTCAACAA
    CGGATAGCTG AACTTGTAGC CTCAGAGTTT TATGACCAAG GGGATAAAGA GAGGAGGGAA
    CTTAATATTG AACCAATAGA CCTGATGAAC AGAGAAAAGA AAGACAAAAT ACCCAGCATG
    CAGGTTGGAT TTATTGATGC AGTCTGCTTA GAACTTTATG AGGCCTTAAC GCAGATCTCT
    GAATCGTGTT ACCCTTTACT TGATGGCTGT CAGAGAAATA GGCAGATGTG GCAGATCTTA
    GCAGAGCAGC AGGAAAAGAA CTTGGTGAAT GGGGCGAAGA GCCAGCCTGA AGTGAAGTGC
    AACATGGATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29652
    Clone ID Related Accession (Same CDS sequence) XM_012966035.2
    Accession Version XM_012966035.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2823bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966035.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCGCCTCT CACTTCCGTC TGCTGCTGCT GGGGTGGATC CGAAGGGCAA TCACCACACA 
    GATGCATTTA GGAAGTTGTG GAAAACCTCC TCACTCGAGA GCTGCCTTTT ATTTATACAT
    TCTTCTTATG TCCCCCCTCC CTGTGCGCTA TTCTTTCGCC GGTTCCCTTC CCCGGCCTGT
    CTTGGAAAGA GAATGTGGCA GGGGAATCTT TACCGCGAAG CCTCAGTGGC CGCTGTTGGT
    TTCCCTCTGG CTGCTGCCCC GGAGATGGGG GACACCCCCG AACCCCAAGT TGAAAACTGG
    GCTGCTACCC ATTCGGCTAC AGTGAACTCC AGCTGCAATG CGCATGCGTG CTGCACTCTA
    CAAGAAATGG TCAACGCATG GTTTGCTGAA CGAGTTCATA CAATACCAGC TAGTAAAGAG
    CAAGGAAAAA ATAATTCAGA GTCTAATCCT CAGGTGTGCT ATTCAGAAAA TACCACTCAG
    GGAACTCCAG TGAGGAAGAT CTCAGCCTCA GAGTTCGATA GGCCCCTAAG GCCAATTGTT
    GTCAAAGATT CTGTCGGAAC ACTGAAATTT CTTTCTGATT CTGAAAAGAA GGAACTGATG
    CCTCTTCAAC CACCCAGTCT GTCCAGAGCA GGAGATCAGT GCTCAAGGCT TTTGGAACTG
    GTGAAAGATA TTTCAAGTCA CCTTGACGTC ACAACACTTT GTCATAAAAT TTTTTTGCAC
    ATCCATGAGC TAATAGCAGC AGATCGTTAT TCGCTCTTTT TGGTTTGTGA GGACAGTTCC
    AATAAGAAGT TTCTGGTCAG TAGACTGTTT GACGTGGCAG AAGGTACAAC TGTGGAACAG
    GCTTCAAACA ATTGCATCCG TTTGGAATGG AATAAAGGGA TTGTTGGTCA CGTGGCAGAG
    TTTGGTCAGC CCTTGAATAT AAAGGATGCT TACCAGGACT CCAGATTCAA TGCGGAAGTC
    GACCAAATTA CTGGGTACAA AACACAAAGT ATTCTTTGTA TGCCAATTAA GAACCACAGG
    GAAGAGGTGG TTGGTGTAGC ACAAGCAATC AATAAGAAGT CAGGAAGCAA TGGGACATTT
    ACTGACCAAG ATGAAAAGGA CTTTGCTGCT TATCTGGCAT TCTGCGGAAT TGTTCTACAC
    AATGCACAAC TTTACGAAAC ATCACTGCTT GAAAACAGGA GGAACCAGGT GCTGCTTGAC
    CTTGCAACCT TGATATTTGA AGAACAGCAA TCACTGGAAG TCATCTTAAA GAAGATCGCA
    GCTACCATTC TGTCATTTAT GCAGGCTCAG AGATGCACAA TATTCATTGT GGATGAAGAT
    AGTCCTGATA CCTTTTCAAG TGTGTTTCAT ATGGAGTCTG ATGAATTAGA AGAGTCTGCA
    GATGCTGTAA AAAGGGAATA TGATGTGAGC AAAATGAATT ACATGTATGC ACAGTATGTA
    GCAAATACAA TGGAAGCAAT TAACATTCCA GACATATGTA AGGACACAAG ATTTCCATGG
    ACAAACGGAA ATGAAGAGAA GAATTTCAAG CATATTCAGA GTTTGCTTTG CTCCCCAATC
    AAGAATGCAA AGAAGAATAA AGTAATAGGA GTCTGTCAAC TTGTAAATAA GGTGGATGAA
    AACTCTGGCA AAATTAAAGC ATTCAACAAA AACGATGAAC AGTTTCTAGA AGCCTTTGCC
    ATATTCTGTG GCTTGGGAAT TCAGAACACC CAGATGTTTG AAGTTGTGGA GCGTGCGATG
    GCCAAACAGA TGGTGACATT AGAGATTCTT TCATATCACG CATCTGCTGC TGAGGAAGAA
    ACAATGGAGC TGCAGGGAGC CATTGTGCCA TCTGCACAAT CGCTTAAACT GACAGAATTC
    TACTTCAGCG ATTTTGAGCT TTCTGACATG GAAACCACAT TAGCCACAAG CCGAATGTTC
    ACAGACCTCA ACCTTGTGCA GACCTTCCAA ATGAAATATG AGACCCTTTG CCGGTGGATC
    CTGAGCGTGA AGAAGAACTA TAGAAAAAAT GTTGCATATC ACAACTGGAG ACATGCCTTT
    AATACAGCCC AGTGTATGTT CGCAGCCCTG AGAACTGGCA AAATTCAGGG TAAGCTAAAT
    GACCTAGAAA TCCTTGCACT GATGATAGCC ACCTTGAGCC ATGACTTGGA CCACAGAGGA
    GTTAACAATT CTTTTATACA GAGAAGTGAA CATCCTCTAG CACAGCTATA CTGTCATTCT
    ATCATGGAGA ACCACCATTT TGACCAGTGC CTAATGATAC TAAACAGTCA GGGTAACCAG
    ATTCTAAGTG GGCTTTCTGT TCAAGACTAC AAGACTGTTC TGAAGATGAT AAAACAAGCC
    ATCCTTGCTA CTGATTTGGC ACTATACATT AAGCGAAGAA GTGATTTCTT TGAATTAGTG
    AATAAGAAGA AATTCAAATG GGATGATCCT TCACAAAAAG AACTGTTCTT AGCAATGCTG
    ATGACGGCTT GTGATCTGTC TGCAATAACA AAGCCATGGC CAGTTCAACA ACGGATAGCT
    GAACTTGTAG CCTCAGAGTT TTATGACCAA GGGGATAAAG AGAGGAGGGA ACTTAATATT
    GAACCAATAG ACCTGATGAA CAGAGAAAAG AAAGACAAAA TACCCAGCAT GCAGGTTGGA
    TTTATTGATG CAGTCTGCTT AGAACTTTAT GAGGCCTTAA CGCAGATCTC TGAATCGTGT
    TACCCTTTAC TTGATGGCTG TCAGAGAAAT AGGCAGATGT GGCAGATCTT AGCAGAGCAG
    CAGGAAAAGA ACTTGGTGAA TGGGGCGAAG AGCCAGCCTG AAGTGAAGTG CAACATGGAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821489.1
    CDS435..3257
    Translation

    Target ORF information:

    RefSeq Version XM_012966035.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphodiesterase 5A (pde5a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966035.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCGCCTCT CACTTCCGTC TGCTGCTGCT GGGGTGGATC CGAAGGGCAA TCACCACACA 
    GATGCATTTA GGAAGTTGTG GAAAACCTCC TCACTCGAGA GCTGCCTTTT ATTTATACAT
    TCTTCTTATG TCCCCCCTCC CTGTGCGCTA TTCTTTCGCC GGTTCCCTTC CCCGGCCTGT
    CTTGGAAAGA GAATGTGGCA GGGGAATCTT TACCGCGAAG CCTCAGTGGC CGCTGTTGGT
    TTCCCTCTGG CTGCTGCCCC GGAGATGGGG GACACCCCCG AACCCCAAGT TGAAAACTGG
    GCTGCTACCC ATTCGGCTAC AGTGAACTCC AGCTGCAATG CGCATGCGTG CTGCACTCTA
    CAAGAAATGG TCAACGCATG GTTTGCTGAA CGAGTTCATA CAATACCAGC TAGTAAAGAG
    CAAGGAAAAA ATAATTCAGA GTCTAATCCT CAGGTGTGCT ATTCAGAAAA TACCACTCAG
    GGAACTCCAG TGAGGAAGAT CTCAGCCTCA GAGTTCGATA GGCCCCTAAG GCCAATTGTT
    GTCAAAGATT CTGTCGGAAC ACTGAAATTT CTTTCTGATT CTGAAAAGAA GGAACTGATG
    CCTCTTCAAC CACCCAGTCT GTCCAGAGCA GGAGATCAGT GCTCAAGGCT TTTGGAACTG
    GTGAAAGATA TTTCAAGTCA CCTTGACGTC ACAACACTTT GTCATAAAAT TTTTTTGCAC
    ATCCATGAGC TAATAGCAGC AGATCGTTAT TCGCTCTTTT TGGTTTGTGA GGACAGTTCC
    AATAAGAAGT TTCTGGTCAG TAGACTGTTT GACGTGGCAG AAGGTACAAC TGTGGAACAG
    GCTTCAAACA ATTGCATCCG TTTGGAATGG AATAAAGGGA TTGTTGGTCA CGTGGCAGAG
    TTTGGTCAGC CCTTGAATAT AAAGGATGCT TACCAGGACT CCAGATTCAA TGCGGAAGTC
    GACCAAATTA CTGGGTACAA AACACAAAGT ATTCTTTGTA TGCCAATTAA GAACCACAGG
    GAAGAGGTGG TTGGTGTAGC ACAAGCAATC AATAAGAAGT CAGGAAGCAA TGGGACATTT
    ACTGACCAAG ATGAAAAGGA CTTTGCTGCT TATCTGGCAT TCTGCGGAAT TGTTCTACAC
    AATGCACAAC TTTACGAAAC ATCACTGCTT GAAAACAGGA GGAACCAGGT GCTGCTTGAC
    CTTGCAACCT TGATATTTGA AGAACAGCAA TCACTGGAAG TCATCTTAAA GAAGATCGCA
    GCTACCATTC TGTCATTTAT GCAGGCTCAG AGATGCACAA TATTCATTGT GGATGAAGAT
    AGTCCTGATA CCTTTTCAAG TGTGTTTCAT ATGGAGTCTG ATGAATTAGA AGAGTCTGCA
    GATGCTGTAA AAAGGGAATA TGATGTGAGC AAAATGAATT ACATGTATGC ACAGTATGTA
    GCAAATACAA TGGAAGCAAT TAACATTCCA GACATATGTA AGGACACAAG ATTTCCATGG
    ACAAACGGAA ATGAAGAGAA GAATTTCAAG CATATTCAGA GTTTGCTTTG CTCCCCAATC
    AAGAATGCAA AGAAGAATAA AGTAATAGGA GTCTGTCAAC TTGTAAATAA GGTGGATGAA
    AACTCTGGCA AAATTAAAGC ATTCAACAAA AACGATGAAC AGTTTCTAGA AGCCTTTGCC
    ATATTCTGTG GCTTGGGAAT TCAGAACACC CAGATGTTTG AAGTTGTGGA GCGTGCGATG
    GCCAAACAGA TGGTGACATT AGAGATTCTT TCATATCACG CATCTGCTGC TGAGGAAGAA
    ACAATGGAGC TGCAGGGAGC CATTGTGCCA TCTGCACAAT CGCTTAAACT GACAGAATTC
    TACTTCAGCG ATTTTGAGCT TTCTGACATG GAAACCACAT TAGCCACAAG CCGAATGTTC
    ACAGACCTCA ACCTTGTGCA GACCTTCCAA ATGAAATATG AGACCCTTTG CCGGTGGATC
    CTGAGCGTGA AGAAGAACTA TAGAAAAAAT GTTGCATATC ACAACTGGAG ACATGCCTTT
    AATACAGCCC AGTGTATGTT CGCAGCCCTG AGAACTGGCA AAATTCAGGG TAAGCTAAAT
    GACCTAGAAA TCCTTGCACT GATGATAGCC ACCTTGAGCC ATGACTTGGA CCACAGAGGA
    GTTAACAATT CTTTTATACA GAGAAGTGAA CATCCTCTAG CACAGCTATA CTGTCATTCT
    ATCATGGAGA ACCACCATTT TGACCAGTGC CTAATGATAC TAAACAGTCA GGGTAACCAG
    ATTCTAAGTG GGCTTTCTGT TCAAGACTAC AAGACTGTTC TGAAGATGAT AAAACAAGCC
    ATCCTTGCTA CTGATTTGGC ACTATACATT AAGCGAAGAA GTGATTTCTT TGAATTAGTG
    AATAAGAAGA AATTCAAATG GGATGATCCT TCACAAAAAG AACTGTTCTT AGCAATGCTG
    ATGACGGCTT GTGATCTGTC TGCAATAACA AAGCCATGGC CAGTTCAACA ACGGATAGCT
    GAACTTGTAG CCTCAGAGTT TTATGACCAA GGGGATAAAG AGAGGAGGGA ACTTAATATT
    GAACCAATAG ACCTGATGAA CAGAGAAAAG AAAGACAAAA TACCCAGCAT GCAGGTTGGA
    TTTATTGATG CAGTCTGCTT AGAACTTTAT GAGGCCTTAA CGCAGATCTC TGAATCGTGT
    TACCCTTTAC TTGATGGCTG TCAGAGAAAT AGGCAGATGT GGCAGATCTT AGCAGAGCAG
    CAGGAAAAGA ACTTGGTGAA TGGGGCGAAG AGCCAGCCTG AAGTGAAGTG CAACATGGAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29653
    Clone ID Related Accession (Same CDS sequence) XM_012966039.2
    Accession Version XM_012966039.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2706bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966039.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCGCCTCT CACTTCCGTC TGCTGCTGCT GGGGTGGATC CGAAGGGCAA TCACCACACA 
    GATGCATTTA GGAAGTTGTG GAAAACCTCC TCACTCGAGA GCTGCCTTTT ATTTATACAT
    TCTTCTTATG TCCCCCCTCC CTGTGCGCTA TTCTTTCGCC GGTTCCCTTC CCCGGCCTGT
    CTTGGAAAGA GAATGTGGCA GGGGAATCTT TACCGCGAAG CCTCAGTGGC CGCTGTTGGT
    TTCCCTCTGG CTGCTGCCCC GGAGATGGGG GACACCCCCG AACCCCAAGT TGAAAACTGG
    GCTGCTACCC ATTCGGCTAC AGTGAACTCC AGCTGCAATG CGCATGCGTG CTGCACTCTA
    CAAGAAATGG TCAACGCATG GTTTGCTGAA CGAGTTCATA CAATACCAGC TAGTAAAGAG
    CAAGGAAAAA ATAATTCAGA GTCTAATCCT CAGGTGTGCT ATTCAGAAAA TACCACTCAG
    GGAACTCCAG TGAGGAAGAT CTCAGCCTCA GAGTTCGATA GGCCCCTAAG GCCAATTGTT
    GTCAAAGATT CTGTCGGAAC ACTGAAATTT CTTTCTGATT CTGAAAAGAA GGAACTGATG
    CCTCTTCAAC CACCCAGTCT GTCCAGAGCA GGAGATCAGT GCTCAAGGCT TTTGGAACTG
    GTGAAAGATA TTTCAAGTCA CCTTGACGTC ACAACACTTT GTCATAAAAT TTTTTTGCAC
    ATCCATGAGC TAATAGCAGC AGATCGTTAT TCGCTCTTTT TGGTTTGTGA GGACAGTTCC
    AATAAGAAGT TTCTGGTCAG TAGACTGTTT GACGTGGCAG AAGGTACAAC TGTGGAACAG
    GCTTCAAACA ATTGCATCCG TTTGGAATGG AATAAAGGGA TTGTTGGTCA CGTGGCAGAG
    TTTGGTCAGC CCTTGAATAT AAAGGATGCT TACCAGGACT CCAGATTCAA TGCGGAAGTC
    GACCAAATTA CTGGGTACAA AACACAAAGT ATTCTTTGTA TGCCAATTAA GAACCACAGG
    GAAGAGGTGG TTGGTGTAGC ACAAGCAATC AATAAGAAGT CAGGAAGCAA TGGGACATTT
    ACTGACCAAG ATGAAAAGGA CTTTGCTGCT TATCTGGCAT TCTGCGGAAT TGTTCTACAC
    AATGCACAAC TTTACGAAAC ATCACTGCTT GAAAACAGGA GGAACCAGGT GCTGCTTGAC
    CTTGCAACCT TGATATTTGA AGAACAGCAA TCACTGGAAG TCATCTTAAA GAAGATCGCA
    GCTACCATTC TGTCATTTAT GCAGGCTCAG AGATGCACAA TATTCATTGT GGATGAAGAT
    AGTCCTGATA CCTTTTCAAG TGTGTTTCAT ATGGAGTCTG ATGAATTAGA AGAGTCTGCA
    GATGCTGTAA AAAGGGAATA TGATGTGAGC AAAATGAATT ACATGTATGC ACAGTATGTA
    GCAAATACAA TGGAAGCAAT TAACATTCCA GACATATGTA AGGACACAAG ATTTCCATGG
    ACAAACGGAA ATGAAGAGAA GAATTTCAAG CATATTCAGA GTTTGCTTTG CTCCCCAATC
    AAGAATGCAA AGAAGAATAA AGTAATAGGA GTCTGTCAAC TTGTAAATAA GGTGGATGAA
    AACTCTGGCA AAATTAAAGC ATTCAACAAA AACGATGAAC AGTTTCTAGA AGCCTTTGCC
    ATATTCTGTG GCTTGGGAAT TCAGAACACC CAGATGTTTG AAGTTGTGGA GCGTGCGATG
    GCCAAACAGA TGGTGACATT AGAGATTCTT TCATATCACG CATCTGCTGC TGAGGAAGAA
    ACAATGGAGC TGCAGGTAAC AGCGGGAGCC ATTGTGCCAT CTGCACAATC GCTTAAACTG
    ACAGAATTCT ACTTCAGCGA TTTTGAGCTT TCTGACATGG AAACCACATT AGCCACAAGC
    CGAATGTTCA CAGACCTCAA CCTTGTGCAG ACCTTCCAAA TGAAATATGA GGGTAAGCTA
    AATGACCTAG AAATCCTTGC ACTGATGATA GCCACCTTGA GCCATGACTT GGACCACAGA
    GGAGTTAACA ATTCTTTTAT ACAGAGAAGT GAACATCCTC TAGCACAGCT ATACTGTCAT
    TCTATCATGG AGAACCACCA TTTTGACCAG TGCCTAATGA TACTAAACAG TCAGGGTAAC
    CAGATTCTAA GTGGGCTTTC TGTTCAAGAC TACAAGACTG TTCTGAAGAT GATAAAACAA
    GCCATCCTTG CTACTGATTT GGCACTATAC ATTAAGCGAA GAAGTGATTT CTTTGAATTA
    GTGAATAAGA AGAAATTCAA ATGGGATGAT CCTTCACAAA AAGAACTGTT CTTAGCAATG
    CTGATGACGG CTTGTGATCT GTCTGCAATA ACAAAGCCAT GGCCAGTTCA ACAACGGATA
    GCTGAACTTG TAGCCTCAGA GTTTTATGAC CAAGGGGATA AAGAGAGGAG GGAACTTAAT
    ATTGAACCAA TAGACCTGAT GAACAGAGAA AAGAAAGACA AAATACCCAG CATGCAGGTT
    GGATTTATTG ATGCAGTCTG CTTAGAACTT TATGAGGCCT TAACGCAGAT CTCTGAATCG
    TGTTACCCTT TACTTGATGG CTGTCAGAGA AATAGGCAGA TGTGGCAGAT CTTAGCAGAG
    CAGCAGGAAA AGAACTTGGT GAATGGGGCG AAGAGCCAGC CTGAAGTGAA GTGCAACATG
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821493.1
    CDS435..3140
    Translation

    Target ORF information:

    RefSeq Version XM_012966039.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphodiesterase 5A (pde5a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966039.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCGCCTCT CACTTCCGTC TGCTGCTGCT GGGGTGGATC CGAAGGGCAA TCACCACACA 
    GATGCATTTA GGAAGTTGTG GAAAACCTCC TCACTCGAGA GCTGCCTTTT ATTTATACAT
    TCTTCTTATG TCCCCCCTCC CTGTGCGCTA TTCTTTCGCC GGTTCCCTTC CCCGGCCTGT
    CTTGGAAAGA GAATGTGGCA GGGGAATCTT TACCGCGAAG CCTCAGTGGC CGCTGTTGGT
    TTCCCTCTGG CTGCTGCCCC GGAGATGGGG GACACCCCCG AACCCCAAGT TGAAAACTGG
    GCTGCTACCC ATTCGGCTAC AGTGAACTCC AGCTGCAATG CGCATGCGTG CTGCACTCTA
    CAAGAAATGG TCAACGCATG GTTTGCTGAA CGAGTTCATA CAATACCAGC TAGTAAAGAG
    CAAGGAAAAA ATAATTCAGA GTCTAATCCT CAGGTGTGCT ATTCAGAAAA TACCACTCAG
    GGAACTCCAG TGAGGAAGAT CTCAGCCTCA GAGTTCGATA GGCCCCTAAG GCCAATTGTT
    GTCAAAGATT CTGTCGGAAC ACTGAAATTT CTTTCTGATT CTGAAAAGAA GGAACTGATG
    CCTCTTCAAC CACCCAGTCT GTCCAGAGCA GGAGATCAGT GCTCAAGGCT TTTGGAACTG
    GTGAAAGATA TTTCAAGTCA CCTTGACGTC ACAACACTTT GTCATAAAAT TTTTTTGCAC
    ATCCATGAGC TAATAGCAGC AGATCGTTAT TCGCTCTTTT TGGTTTGTGA GGACAGTTCC
    AATAAGAAGT TTCTGGTCAG TAGACTGTTT GACGTGGCAG AAGGTACAAC TGTGGAACAG
    GCTTCAAACA ATTGCATCCG TTTGGAATGG AATAAAGGGA TTGTTGGTCA CGTGGCAGAG
    TTTGGTCAGC CCTTGAATAT AAAGGATGCT TACCAGGACT CCAGATTCAA TGCGGAAGTC
    GACCAAATTA CTGGGTACAA AACACAAAGT ATTCTTTGTA TGCCAATTAA GAACCACAGG
    GAAGAGGTGG TTGGTGTAGC ACAAGCAATC AATAAGAAGT CAGGAAGCAA TGGGACATTT
    ACTGACCAAG ATGAAAAGGA CTTTGCTGCT TATCTGGCAT TCTGCGGAAT TGTTCTACAC
    AATGCACAAC TTTACGAAAC ATCACTGCTT GAAAACAGGA GGAACCAGGT GCTGCTTGAC
    CTTGCAACCT TGATATTTGA AGAACAGCAA TCACTGGAAG TCATCTTAAA GAAGATCGCA
    GCTACCATTC TGTCATTTAT GCAGGCTCAG AGATGCACAA TATTCATTGT GGATGAAGAT
    AGTCCTGATA CCTTTTCAAG TGTGTTTCAT ATGGAGTCTG ATGAATTAGA AGAGTCTGCA
    GATGCTGTAA AAAGGGAATA TGATGTGAGC AAAATGAATT ACATGTATGC ACAGTATGTA
    GCAAATACAA TGGAAGCAAT TAACATTCCA GACATATGTA AGGACACAAG ATTTCCATGG
    ACAAACGGAA ATGAAGAGAA GAATTTCAAG CATATTCAGA GTTTGCTTTG CTCCCCAATC
    AAGAATGCAA AGAAGAATAA AGTAATAGGA GTCTGTCAAC TTGTAAATAA GGTGGATGAA
    AACTCTGGCA AAATTAAAGC ATTCAACAAA AACGATGAAC AGTTTCTAGA AGCCTTTGCC
    ATATTCTGTG GCTTGGGAAT TCAGAACACC CAGATGTTTG AAGTTGTGGA GCGTGCGATG
    GCCAAACAGA TGGTGACATT AGAGATTCTT TCATATCACG CATCTGCTGC TGAGGAAGAA
    ACAATGGAGC TGCAGGTAAC AGCGGGAGCC ATTGTGCCAT CTGCACAATC GCTTAAACTG
    ACAGAATTCT ACTTCAGCGA TTTTGAGCTT TCTGACATGG AAACCACATT AGCCACAAGC
    CGAATGTTCA CAGACCTCAA CCTTGTGCAG ACCTTCCAAA TGAAATATGA GGGTAAGCTA
    AATGACCTAG AAATCCTTGC ACTGATGATA GCCACCTTGA GCCATGACTT GGACCACAGA
    GGAGTTAACA ATTCTTTTAT ACAGAGAAGT GAACATCCTC TAGCACAGCT ATACTGTCAT
    TCTATCATGG AGAACCACCA TTTTGACCAG TGCCTAATGA TACTAAACAG TCAGGGTAAC
    CAGATTCTAA GTGGGCTTTC TGTTCAAGAC TACAAGACTG TTCTGAAGAT GATAAAACAA
    GCCATCCTTG CTACTGATTT GGCACTATAC ATTAAGCGAA GAAGTGATTT CTTTGAATTA
    GTGAATAAGA AGAAATTCAA ATGGGATGAT CCTTCACAAA AAGAACTGTT CTTAGCAATG
    CTGATGACGG CTTGTGATCT GTCTGCAATA ACAAAGCCAT GGCCAGTTCA ACAACGGATA
    GCTGAACTTG TAGCCTCAGA GTTTTATGAC CAAGGGGATA AAGAGAGGAG GGAACTTAAT
    ATTGAACCAA TAGACCTGAT GAACAGAGAA AAGAAAGACA AAATACCCAG CATGCAGGTT
    GGATTTATTG ATGCAGTCTG CTTAGAACTT TATGAGGCCT TAACGCAGAT CTCTGAATCG
    TGTTACCCTT TACTTGATGG CTGTCAGAGA AATAGGCAGA TGTGGCAGAT CTTAGCAGAG
    CAGCAGGAAA AGAACTTGGT GAATGGGGCG AAGAGCCAGC CTGAAGTGAA GTGCAACATG
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29654
    Clone ID Related Accession (Same CDS sequence) XM_012966045.2
    Accession Version XM_012966045.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGGGAA GCTGCAGCCT GCAAAGCCAG GAGCAGGAGG GCATAGAGTC GTGGCTGGAC 
    GATCACTGGG ACTTCACACA CTCGTACTTT GTGAGGAAAG CCACAAGAGA AATGGTCAAC
    GCATGGTTTG CTGAACGAGT TCATACAATA CCAGCTAGTA AAGAGCAAGG AAAAAATAAT
    TCAGAGTCTA ATCCTCAGGT GTGCTATTCA GAAAATACCA CTCAGGGAAC TCCAGTGAGG
    AAGATCTCAG CCTCAGAGTT CGATAGGCCC CTAAGGCCAA TTGTTGTCAA AGATTCTGTC
    GGAACACTGA AATTTCTTTC TGATTCTGAA AAGAAGGAAC TGATGCCTCT TCAACCACCC
    AGTCTGTCCA GAGCAGGAGA TCAGTGCTCA AGGCTTTTGG AACTGGTGAA AGATATTTCA
    AGTCACCTTG ACGTCACAAC ACTTTGTCAT AAAATTTTTT TGCACATCCA TGAGCTAATA
    GCAGCAGATC GTTATTCGCT CTTTTTGGTT TGTGAGGACA GTTCCAATAA GAAGTTTCTG
    GTCAGTAGAC TGTTTGACGT GGCAGAAGGT ACAACTGTGG AACAGGCTTC AAACAATTGC
    ATCCGTTTGG AATGGAATAA AGGGATTGTT GGTCACGTGG CAGAGTTTGG TCAGCCCTTG
    AATATAAAGG ATGCTTACCA GGACTCCAGA TTCAATGCGG AAGTCGACCA AATTACTGGG
    TACAAAACAC AAAGTATTCT TTGTATGCCA ATTAAGAACC ACAGGGAAGA GGTGGTTGGT
    GTAGCACAAG CAATCAATAA GAAGTCAGGA AGCAATGGGA CATTTACTGA CCAAGATGAA
    AAGGACTTTG CTGCTTATCT GGCATTCTGC GGAATTGTTC TACACAATGC ACAACTTTAC
    GAAACATCAC TGCTTGAAAA CAGGAGGAAC CAGGTGCTGC TTGACCTTGC AACCTTGATA
    TTTGAAGAAC AGCAATCACT GGAAGTCATC TTAAAGAAGA TCGCAGCTAC CATTCTGTCA
    TTTATGCAGG CTCAGAGATG CACAATATTC ATTGTGGATG AAGATAGTCC TGATACCTTT
    TCAAGTGTGT TTCATATGGA GTCTGATGAA TTAGAAGAGT CTGCAGATGC TGTAAAAAGG
    GAATATGATG TGAGCAAAAT GAATTACATG TATGCACAGT ATGTAGCAAA TACAATGGAA
    GCAATTAACA TTCCAGACAT ATGTAAGGAC ACAAGATTTC CATGGACAAA CGGAAATGAA
    GAGAAGAATT TCAAGCATAT TCAGAGTTTG CTTTGCTCCC CAATCAAGAA TGCAAAGAAG
    AATAAAGTAA TAGGAGTCTG TCAACTTGTA AATAAGGTGG ATGAAAACTC TGGCAAAATT
    AAAGCATTCA ACAAAAACGA TGAACAGTTT CTAGAAGCCT TTGCCATATT CTGTGGCTTG
    GGAATTCAGA ACACCCAGAT GTTTGAAGTT GTGGAGCGTG CGATGGCCAA ACAGATGGTG
    ACATTAGAGA TTCTTTCATA TCACGCATCT GCTGCTGAGG AAGAAACAAT GGAGCTGCAG
    GTAACAGCGG GAGCCATTGT GCCATCTGCA CAATCGCTTA AACTGACAGA ATTCTACTTC
    AGCGATTTTG AGCTTTCTGA CATGGAAACC ACATTAGCCA CAAGCCGAAT GTTCACAGAC
    CTCAACCTTG TGCAGACCTT CCAAATGAAA TATGAGACCC TTTGCCGGTG GATCCTGAGC
    GTGAAGAAGA ACTATAGAAA AAATGTTGCA TATCACAACT GGAGACATGC CTTTAATACA
    GCCCAGTGTA TGTTCGCAGC CCTGAGAACT GGCAAAATTC AGGGTAAGCT AAATGACCTA
    GAAATCCTTG CACTGATGAT AGCCACCTTG AGCCATGACT TGGACCACAG AGGAGTTAAC
    AATTCTTTTA TACAGAGAAG TGAACATCCT CTAGCACAGC TATACTGTCA TTCTATCATG
    GAGAACCACC ATTTTGACCA GTGCCTAATG ATACTAAACA GTCAGGGTAA CCAGATTCTA
    AGTGGGCTTT CTGTTCAAGA CTACAAGACT GTTCTGAAGA TGATAAAACA AGCCATCCTT
    GCTACTGATT TGGCACTATA CATTAAGCGA AGAAGTGATT TCTTTGAATT AGTGAATAAG
    AAGAAATTCA AATGGGATGA TCCTTCACAA AAAGAACTGT TCTTAGCAAT GCTGATGACG
    GCTTGTGATC TGTCTGCAAT AACAAAGCCA TGGCCAGTTC AACAACGGAT AGCTGAACTT
    GTAGCCTCAG AGTTTTATGA CCAAGGGGAT AAAGAGAGGA GGGAACTTAA TATTGAACCA
    ATAGACCTGA TGAACAGAGA AAAGAAAGAC AAAATACCCA GCATGCAGGT TGGATTTATT
    GATGCAGTCT GCTTAGAACT TTATGAGGCC TTAACGCAGA TCTCTGAATC GTGTTACCCT
    TTACTTGATG GCTGTCAGAG AAATAGGCAG ATGTGGCAGA TCTTAGCAGA GCAGCAGGAA
    AAGAACTTGG TGAATGGGGC GAAGAGCCAG CCTGAAGTGA AGTGCAACAT GGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821499.1
    CDS374..2950
    Translation

    Target ORF information:

    RefSeq Version XM_012966045.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphodiesterase 5A (pde5a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966045.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGGGAA GCTGCAGCCT GCAAAGCCAG GAGCAGGAGG GCATAGAGTC GTGGCTGGAC 
    GATCACTGGG ACTTCACACA CTCGTACTTT GTGAGGAAAG CCACAAGAGA AATGGTCAAC
    GCATGGTTTG CTGAACGAGT TCATACAATA CCAGCTAGTA AAGAGCAAGG AAAAAATAAT
    TCAGAGTCTA ATCCTCAGGT GTGCTATTCA GAAAATACCA CTCAGGGAAC TCCAGTGAGG
    AAGATCTCAG CCTCAGAGTT CGATAGGCCC CTAAGGCCAA TTGTTGTCAA AGATTCTGTC
    GGAACACTGA AATTTCTTTC TGATTCTGAA AAGAAGGAAC TGATGCCTCT TCAACCACCC
    AGTCTGTCCA GAGCAGGAGA TCAGTGCTCA AGGCTTTTGG AACTGGTGAA AGATATTTCA
    AGTCACCTTG ACGTCACAAC ACTTTGTCAT AAAATTTTTT TGCACATCCA TGAGCTAATA
    GCAGCAGATC GTTATTCGCT CTTTTTGGTT TGTGAGGACA GTTCCAATAA GAAGTTTCTG
    GTCAGTAGAC TGTTTGACGT GGCAGAAGGT ACAACTGTGG AACAGGCTTC AAACAATTGC
    ATCCGTTTGG AATGGAATAA AGGGATTGTT GGTCACGTGG CAGAGTTTGG TCAGCCCTTG
    AATATAAAGG ATGCTTACCA GGACTCCAGA TTCAATGCGG AAGTCGACCA AATTACTGGG
    TACAAAACAC AAAGTATTCT TTGTATGCCA ATTAAGAACC ACAGGGAAGA GGTGGTTGGT
    GTAGCACAAG CAATCAATAA GAAGTCAGGA AGCAATGGGA CATTTACTGA CCAAGATGAA
    AAGGACTTTG CTGCTTATCT GGCATTCTGC GGAATTGTTC TACACAATGC ACAACTTTAC
    GAAACATCAC TGCTTGAAAA CAGGAGGAAC CAGGTGCTGC TTGACCTTGC AACCTTGATA
    TTTGAAGAAC AGCAATCACT GGAAGTCATC TTAAAGAAGA TCGCAGCTAC CATTCTGTCA
    TTTATGCAGG CTCAGAGATG CACAATATTC ATTGTGGATG AAGATAGTCC TGATACCTTT
    TCAAGTGTGT TTCATATGGA GTCTGATGAA TTAGAAGAGT CTGCAGATGC TGTAAAAAGG
    GAATATGATG TGAGCAAAAT GAATTACATG TATGCACAGT ATGTAGCAAA TACAATGGAA
    GCAATTAACA TTCCAGACAT ATGTAAGGAC ACAAGATTTC CATGGACAAA CGGAAATGAA
    GAGAAGAATT TCAAGCATAT TCAGAGTTTG CTTTGCTCCC CAATCAAGAA TGCAAAGAAG
    AATAAAGTAA TAGGAGTCTG TCAACTTGTA AATAAGGTGG ATGAAAACTC TGGCAAAATT
    AAAGCATTCA ACAAAAACGA TGAACAGTTT CTAGAAGCCT TTGCCATATT CTGTGGCTTG
    GGAATTCAGA ACACCCAGAT GTTTGAAGTT GTGGAGCGTG CGATGGCCAA ACAGATGGTG
    ACATTAGAGA TTCTTTCATA TCACGCATCT GCTGCTGAGG AAGAAACAAT GGAGCTGCAG
    GTAACAGCGG GAGCCATTGT GCCATCTGCA CAATCGCTTA AACTGACAGA ATTCTACTTC
    AGCGATTTTG AGCTTTCTGA CATGGAAACC ACATTAGCCA CAAGCCGAAT GTTCACAGAC
    CTCAACCTTG TGCAGACCTT CCAAATGAAA TATGAGACCC TTTGCCGGTG GATCCTGAGC
    GTGAAGAAGA ACTATAGAAA AAATGTTGCA TATCACAACT GGAGACATGC CTTTAATACA
    GCCCAGTGTA TGTTCGCAGC CCTGAGAACT GGCAAAATTC AGGGTAAGCT AAATGACCTA
    GAAATCCTTG CACTGATGAT AGCCACCTTG AGCCATGACT TGGACCACAG AGGAGTTAAC
    AATTCTTTTA TACAGAGAAG TGAACATCCT CTAGCACAGC TATACTGTCA TTCTATCATG
    GAGAACCACC ATTTTGACCA GTGCCTAATG ATACTAAACA GTCAGGGTAA CCAGATTCTA
    AGTGGGCTTT CTGTTCAAGA CTACAAGACT GTTCTGAAGA TGATAAAACA AGCCATCCTT
    GCTACTGATT TGGCACTATA CATTAAGCGA AGAAGTGATT TCTTTGAATT AGTGAATAAG
    AAGAAATTCA AATGGGATGA TCCTTCACAA AAAGAACTGT TCTTAGCAAT GCTGATGACG
    GCTTGTGATC TGTCTGCAAT AACAAAGCCA TGGCCAGTTC AACAACGGAT AGCTGAACTT
    GTAGCCTCAG AGTTTTATGA CCAAGGGGAT AAAGAGAGGA GGGAACTTAA TATTGAACCA
    ATAGACCTGA TGAACAGAGA AAAGAAAGAC AAAATACCCA GCATGCAGGT TGGATTTATT
    GATGCAGTCT GCTTAGAACT TTATGAGGCC TTAACGCAGA TCTCTGAATC GTGTTACCCT
    TTACTTGATG GCTGTCAGAG AAATAGGCAG ATGTGGCAGA TCTTAGCAGA GCAGCAGGAA
    AAGAACTTGG TGAATGGGGC GAAGAGCCAG CCTGAAGTGA AGTGCAACAT GGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48073
    Clone ID Related Accession (Same CDS sequence) XM_012966026.3
    Accession Version XM_012966026.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2832bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966026.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCGCCTCT CACTTCCGTC TGCTGCTGCT GGGGTGGATC CGAAGGGCAA TCACCACACA 
    TATGCATTTA GGAAGTTGTG GAAAACCTCC TCACTCGAGA GCTGCCTTTT ATTTATACAT
    TCTTCTTATG TCCCCCCTCC CTGTGCGCTA TTCTTTCGCC GGTTCCCTTC CCCGGCCTGT
    CTTGGAAAGA GAATGTGGCA GGGGAATCTT TACCGCGAAG CCTCAGTGGC CGCTGTTGGT
    TTCCCTCTGG CTGCTGCCCC GGAGATGGGG GACACCCCCG AACCCCAAGT TGAAAACTGG
    GCTGCTACCC ATTCGGCTAC AGTGAACTCC AGCTGCAATG CGCATGCGTG CTGCACTCTA
    CAAGAAATGG TCAACGCATG GTTTGCTGAA CGAGTTCATA CAATACCAGC TAGTAAAGAG
    CAAGGAAAAA ATAATTCAGA GTCTAATCCT CAGGTGTGCT ATTCAGAAAA TACCACTCAG
    GGAACTCCAG TGAGGAAGAT CTCAGCCTCA GAGTTCGATA GGCCCCTAAG GCCAATTGTT
    GTCAAAGATT CTGTCGGAAC ACTGAAATTT CTTTCTGATT CTGAAAAGAA GGAACTGATG
    CCTCTTCAAC CACCCAGTCT GTCCAGAGCA GGAGATCAGT GCTCAAGGCT TTTGGAACTG
    GTGAAAGATA TTTCAAGTCA CCTTGACGTC ACAACACTTT GTCATAAAAT TTTTTTGCAC
    ATCCATGAGC TAATAGCAGC AGATCGTTAT TCGCTCTTTT TGGTTTGTGA GGACAGTTCC
    AATAAGAAGT TTCTGGTCAG TAGACTGTTT GACGTGGCAG AAGGTACAAC TGTGGAACAG
    GCTTCAAACA ATTGCATCCG TTTGGAATGG AATAAAGGGA TTGTTGGTCA CGTGGCAGAG
    TTTGGTCAGC CCTTGAATAT AAAGGATGCT TACCAGGACT CCAGATTCAA TGCGGAAGTC
    GACCAAATTA CTGGGTACAA AACACAAAGT ATTCTTTGTA TGCCAATTAA GAACCACAGG
    GAAGAGGTGG TTGGTGTAGC ACAAGCAATC AATAAGAAGT CAGGAAGCAA TGGGACATTT
    ACTGACCAAG ATGAAAAGGA CTTTGCTGCT TATCTGGCAT TCTGCGGAAT TGTTCTACAC
    AATGCACAAC TTTACGAAAC ATCACTGCTT GAAAACAGGA GGAACCAGGT GCTGCTTGAC
    CTTGCAACCT TGATATTTGA AGAACAGCAA TCACTGGAAG TCATCTTAAA GAAGATCGCA
    GCTACCATTC TGTCATTTAT GCAGGCTCAG AGATGCACAA TATTCATTGT GGATGAAGAT
    AGTCCTGATA CCTTTTCAAG TGTGTTTCAT ATGGAGTCTG ATGAATTAGA AGAGTCTGCA
    GATGCTGTAA AAAGGGAATA TGATGTGAGC AAAATGAATT ACATGTATGC ACAGTATGTA
    GCAAATACAA TGGAAGCAAT TAACATTCCA GACATATGTA AGGACACAAG ATTTCCATGG
    ACAAACGGAA ATGAAGAGAA GAATTTCAAG CATATTCAGA GTTTGCTTTG CTCCCCAATC
    AAGAATGCAA AGAAGAATAA AGTAATAGGA GTCTGTCAAC TTGTAAATAA GGTGGATGAA
    AACTCTGGCA AAATTAAAGC ATTCAACAAA AACGATGAAC AGTTTCTAGA AGCCTTTGCC
    ATATTCTGTG GCTTGGGAAT TCAGAACACC CAGATGTTTG AAGTTGTGGA GCGTGCGATG
    GCCAAACAGA TGGTGACATT AGAGATTCTT TCATATCACG CATCTGCTGC TGAGGAAGAA
    ACAATGGAGC TGCAGGTAAC AGCGGGAGCC ATTGTGCCAT CTGCACAATC GCTTAAACTG
    ACAGAATTCT ACTTCAGCGA TTTTGAGCTT TCTGACATGG AAACCACATT AGCCACAAGC
    CGAATGTTCA CAGACCTCAA CCTTGTGCAG ACCTTCCAAA TGAAATATGA GACCCTTTGC
    CGGTGGATCC TGAGCGTGAA GAAGAACTAT AGAAAAAATG TTGCATATCA CAACTGGAGA
    CATGCCTTTA ATACAGCCCA GTGTATGTTC GCAGCCCTGA GAACTGGCAA AATTCAGGGT
    AAGCTAAATG ACCTAGAAAT CCTTGCACTG ATGATAGCCA CCTTGAGCCA TGACTTGGAC
    CACAGAGGAG TTAACAATTC TTTTATACAG AGAAGTGAAC ATCCTCTAGC ACAGCTATAC
    TGTCATTCTA TCATGGAGAA CCACCATTTT GACCAGTGCC TAATGATACT AAACAGTCAG
    GGTAACCAGA TTCTAAGTGG GCTTTCTGTT CAAGACTACA AGACTGTTCT GAAGATGATA
    AAACAAGCCA TCCTTGCTAC TGATTTGGCA CTATACATTA AGCGAAGAAG TGATTTCTTT
    GAATTAGTGA ATAAGAAGAA ATTCAAATGG GATGATCCTT CACAAAAAGA ACTGTTCTTA
    GCAATGCTGA TGACGGCTTG TGATCTGTCT GCAATAACAA AGCCATGGCC AGTTCAACAA
    CGGATAGCTG AACTTGTAGC CTCAGAGTTT TATGACCAAG GGGATAAAGA GAGGAGGGAA
    CTTAATATTG AACCAATAGA CCTGATGAAC AGAGAAAAGA AAGACAAAAT ACCCAGCATG
    CAGGTTGGAT TTATTGACGC AGTCTGCTTA GAACTTTATG AGGCCTTAAC GCAGATCTCT
    GAATCGTGTT ACCCTTTACT TGATGGCTGT CAGAGAAATA GGCAGATGTG GCAGATCTTA
    GCAGAGCAGC AGGAAAAGAA CTTGGTGAAT GGGGCGAAGA GCCAGCCTGA AGTGAAGTGC
    AACATGGATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821480.2
    CDS30..2861
    Translation

    Target ORF information:

    RefSeq Version XM_012966026.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphodiesterase 5A (pde5a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966026.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCGCCTCT CACTTCCGTC TGCTGCTGCT GGGGTGGATC CGAAGGGCAA TCACCACACA 
    TATGCATTTA GGAAGTTGTG GAAAACCTCC TCACTCGAGA GCTGCCTTTT ATTTATACAT
    TCTTCTTATG TCCCCCCTCC CTGTGCGCTA TTCTTTCGCC GGTTCCCTTC CCCGGCCTGT
    CTTGGAAAGA GAATGTGGCA GGGGAATCTT TACCGCGAAG CCTCAGTGGC CGCTGTTGGT
    TTCCCTCTGG CTGCTGCCCC GGAGATGGGG GACACCCCCG AACCCCAAGT TGAAAACTGG
    GCTGCTACCC ATTCGGCTAC AGTGAACTCC AGCTGCAATG CGCATGCGTG CTGCACTCTA
    CAAGAAATGG TCAACGCATG GTTTGCTGAA CGAGTTCATA CAATACCAGC TAGTAAAGAG
    CAAGGAAAAA ATAATTCAGA GTCTAATCCT CAGGTGTGCT ATTCAGAAAA TACCACTCAG
    GGAACTCCAG TGAGGAAGAT CTCAGCCTCA GAGTTCGATA GGCCCCTAAG GCCAATTGTT
    GTCAAAGATT CTGTCGGAAC ACTGAAATTT CTTTCTGATT CTGAAAAGAA GGAACTGATG
    CCTCTTCAAC CACCCAGTCT GTCCAGAGCA GGAGATCAGT GCTCAAGGCT TTTGGAACTG
    GTGAAAGATA TTTCAAGTCA CCTTGACGTC ACAACACTTT GTCATAAAAT TTTTTTGCAC
    ATCCATGAGC TAATAGCAGC AGATCGTTAT TCGCTCTTTT TGGTTTGTGA GGACAGTTCC
    AATAAGAAGT TTCTGGTCAG TAGACTGTTT GACGTGGCAG AAGGTACAAC TGTGGAACAG
    GCTTCAAACA ATTGCATCCG TTTGGAATGG AATAAAGGGA TTGTTGGTCA CGTGGCAGAG
    TTTGGTCAGC CCTTGAATAT AAAGGATGCT TACCAGGACT CCAGATTCAA TGCGGAAGTC
    GACCAAATTA CTGGGTACAA AACACAAAGT ATTCTTTGTA TGCCAATTAA GAACCACAGG
    GAAGAGGTGG TTGGTGTAGC ACAAGCAATC AATAAGAAGT CAGGAAGCAA TGGGACATTT
    ACTGACCAAG ATGAAAAGGA CTTTGCTGCT TATCTGGCAT TCTGCGGAAT TGTTCTACAC
    AATGCACAAC TTTACGAAAC ATCACTGCTT GAAAACAGGA GGAACCAGGT GCTGCTTGAC
    CTTGCAACCT TGATATTTGA AGAACAGCAA TCACTGGAAG TCATCTTAAA GAAGATCGCA
    GCTACCATTC TGTCATTTAT GCAGGCTCAG AGATGCACAA TATTCATTGT GGATGAAGAT
    AGTCCTGATA CCTTTTCAAG TGTGTTTCAT ATGGAGTCTG ATGAATTAGA AGAGTCTGCA
    GATGCTGTAA AAAGGGAATA TGATGTGAGC AAAATGAATT ACATGTATGC ACAGTATGTA
    GCAAATACAA TGGAAGCAAT TAACATTCCA GACATATGTA AGGACACAAG ATTTCCATGG
    ACAAACGGAA ATGAAGAGAA GAATTTCAAG CATATTCAGA GTTTGCTTTG CTCCCCAATC
    AAGAATGCAA AGAAGAATAA AGTAATAGGA GTCTGTCAAC TTGTAAATAA GGTGGATGAA
    AACTCTGGCA AAATTAAAGC ATTCAACAAA AACGATGAAC AGTTTCTAGA AGCCTTTGCC
    ATATTCTGTG GCTTGGGAAT TCAGAACACC CAGATGTTTG AAGTTGTGGA GCGTGCGATG
    GCCAAACAGA TGGTGACATT AGAGATTCTT TCATATCACG CATCTGCTGC TGAGGAAGAA
    ACAATGGAGC TGCAGGTAAC AGCGGGAGCC ATTGTGCCAT CTGCACAATC GCTTAAACTG
    ACAGAATTCT ACTTCAGCGA TTTTGAGCTT TCTGACATGG AAACCACATT AGCCACAAGC
    CGAATGTTCA CAGACCTCAA CCTTGTGCAG ACCTTCCAAA TGAAATATGA GACCCTTTGC
    CGGTGGATCC TGAGCGTGAA GAAGAACTAT AGAAAAAATG TTGCATATCA CAACTGGAGA
    CATGCCTTTA ATACAGCCCA GTGTATGTTC GCAGCCCTGA GAACTGGCAA AATTCAGGGT
    AAGCTAAATG ACCTAGAAAT CCTTGCACTG ATGATAGCCA CCTTGAGCCA TGACTTGGAC
    CACAGAGGAG TTAACAATTC TTTTATACAG AGAAGTGAAC ATCCTCTAGC ACAGCTATAC
    TGTCATTCTA TCATGGAGAA CCACCATTTT GACCAGTGCC TAATGATACT AAACAGTCAG
    GGTAACCAGA TTCTAAGTGG GCTTTCTGTT CAAGACTACA AGACTGTTCT GAAGATGATA
    AAACAAGCCA TCCTTGCTAC TGATTTGGCA CTATACATTA AGCGAAGAAG TGATTTCTTT
    GAATTAGTGA ATAAGAAGAA ATTCAAATGG GATGATCCTT CACAAAAAGA ACTGTTCTTA
    GCAATGCTGA TGACGGCTTG TGATCTGTCT GCAATAACAA AGCCATGGCC AGTTCAACAA
    CGGATAGCTG AACTTGTAGC CTCAGAGTTT TATGACCAAG GGGATAAAGA GAGGAGGGAA
    CTTAATATTG AACCAATAGA CCTGATGAAC AGAGAAAAGA AAGACAAAAT ACCCAGCATG
    CAGGTTGGAT TTATTGACGC AGTCTGCTTA GAACTTTATG AGGCCTTAAC GCAGATCTCT
    GAATCGTGTT ACCCTTTACT TGATGGCTGT CAGAGAAATA GGCAGATGTG GCAGATCTTA
    GCAGAGCAGC AGGAAAAGAA CTTGGTGAAT GGGGCGAAGA GCCAGCCTGA AGTGAAGTGC
    AACATGGATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48074
    Clone ID Related Accession (Same CDS sequence) XM_012966035.3
    Accession Version XM_012966035.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2823bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966035.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCGCCTCT CACTTCCGTC TGCTGCTGCT GGGGTGGATC CGAAGGGCAA TCACCACACA 
    TATGCATTTA GGAAGTTGTG GAAAACCTCC TCACTCGAGA GCTGCCTTTT ATTTATACAT
    TCTTCTTATG TCCCCCCTCC CTGTGCGCTA TTCTTTCGCC GGTTCCCTTC CCCGGCCTGT
    CTTGGAAAGA GAATGTGGCA GGGGAATCTT TACCGCGAAG CCTCAGTGGC CGCTGTTGGT
    TTCCCTCTGG CTGCTGCCCC GGAGATGGGG GACACCCCCG AACCCCAAGT TGAAAACTGG
    GCTGCTACCC ATTCGGCTAC AGTGAACTCC AGCTGCAATG CGCATGCGTG CTGCACTCTA
    CAAGAAATGG TCAACGCATG GTTTGCTGAA CGAGTTCATA CAATACCAGC TAGTAAAGAG
    CAAGGAAAAA ATAATTCAGA GTCTAATCCT CAGGTGTGCT ATTCAGAAAA TACCACTCAG
    GGAACTCCAG TGAGGAAGAT CTCAGCCTCA GAGTTCGATA GGCCCCTAAG GCCAATTGTT
    GTCAAAGATT CTGTCGGAAC ACTGAAATTT CTTTCTGATT CTGAAAAGAA GGAACTGATG
    CCTCTTCAAC CACCCAGTCT GTCCAGAGCA GGAGATCAGT GCTCAAGGCT TTTGGAACTG
    GTGAAAGATA TTTCAAGTCA CCTTGACGTC ACAACACTTT GTCATAAAAT TTTTTTGCAC
    ATCCATGAGC TAATAGCAGC AGATCGTTAT TCGCTCTTTT TGGTTTGTGA GGACAGTTCC
    AATAAGAAGT TTCTGGTCAG TAGACTGTTT GACGTGGCAG AAGGTACAAC TGTGGAACAG
    GCTTCAAACA ATTGCATCCG TTTGGAATGG AATAAAGGGA TTGTTGGTCA CGTGGCAGAG
    TTTGGTCAGC CCTTGAATAT AAAGGATGCT TACCAGGACT CCAGATTCAA TGCGGAAGTC
    GACCAAATTA CTGGGTACAA AACACAAAGT ATTCTTTGTA TGCCAATTAA GAACCACAGG
    GAAGAGGTGG TTGGTGTAGC ACAAGCAATC AATAAGAAGT CAGGAAGCAA TGGGACATTT
    ACTGACCAAG ATGAAAAGGA CTTTGCTGCT TATCTGGCAT TCTGCGGAAT TGTTCTACAC
    AATGCACAAC TTTACGAAAC ATCACTGCTT GAAAACAGGA GGAACCAGGT GCTGCTTGAC
    CTTGCAACCT TGATATTTGA AGAACAGCAA TCACTGGAAG TCATCTTAAA GAAGATCGCA
    GCTACCATTC TGTCATTTAT GCAGGCTCAG AGATGCACAA TATTCATTGT GGATGAAGAT
    AGTCCTGATA CCTTTTCAAG TGTGTTTCAT ATGGAGTCTG ATGAATTAGA AGAGTCTGCA
    GATGCTGTAA AAAGGGAATA TGATGTGAGC AAAATGAATT ACATGTATGC ACAGTATGTA
    GCAAATACAA TGGAAGCAAT TAACATTCCA GACATATGTA AGGACACAAG ATTTCCATGG
    ACAAACGGAA ATGAAGAGAA GAATTTCAAG CATATTCAGA GTTTGCTTTG CTCCCCAATC
    AAGAATGCAA AGAAGAATAA AGTAATAGGA GTCTGTCAAC TTGTAAATAA GGTGGATGAA
    AACTCTGGCA AAATTAAAGC ATTCAACAAA AACGATGAAC AGTTTCTAGA AGCCTTTGCC
    ATATTCTGTG GCTTGGGAAT TCAGAACACC CAGATGTTTG AAGTTGTGGA GCGTGCGATG
    GCCAAACAGA TGGTGACATT AGAGATTCTT TCATATCACG CATCTGCTGC TGAGGAAGAA
    ACAATGGAGC TGCAGGGAGC CATTGTGCCA TCTGCACAAT CGCTTAAACT GACAGAATTC
    TACTTCAGCG ATTTTGAGCT TTCTGACATG GAAACCACAT TAGCCACAAG CCGAATGTTC
    ACAGACCTCA ACCTTGTGCA GACCTTCCAA ATGAAATATG AGACCCTTTG CCGGTGGATC
    CTGAGCGTGA AGAAGAACTA TAGAAAAAAT GTTGCATATC ACAACTGGAG ACATGCCTTT
    AATACAGCCC AGTGTATGTT CGCAGCCCTG AGAACTGGCA AAATTCAGGG TAAGCTAAAT
    GACCTAGAAA TCCTTGCACT GATGATAGCC ACCTTGAGCC ATGACTTGGA CCACAGAGGA
    GTTAACAATT CTTTTATACA GAGAAGTGAA CATCCTCTAG CACAGCTATA CTGTCATTCT
    ATCATGGAGA ACCACCATTT TGACCAGTGC CTAATGATAC TAAACAGTCA GGGTAACCAG
    ATTCTAAGTG GGCTTTCTGT TCAAGACTAC AAGACTGTTC TGAAGATGAT AAAACAAGCC
    ATCCTTGCTA CTGATTTGGC ACTATACATT AAGCGAAGAA GTGATTTCTT TGAATTAGTG
    AATAAGAAGA AATTCAAATG GGATGATCCT TCACAAAAAG AACTGTTCTT AGCAATGCTG
    ATGACGGCTT GTGATCTGTC TGCAATAACA AAGCCATGGC CAGTTCAACA ACGGATAGCT
    GAACTTGTAG CCTCAGAGTT TTATGACCAA GGGGATAAAG AGAGGAGGGA ACTTAATATT
    GAACCAATAG ACCTGATGAA CAGAGAAAAG AAAGACAAAA TACCCAGCAT GCAGGTTGGA
    TTTATTGACG CAGTCTGCTT AGAACTTTAT GAGGCCTTAA CGCAGATCTC TGAATCGTGT
    TACCCTTTAC TTGATGGCTG TCAGAGAAAT AGGCAGATGT GGCAGATCTT AGCAGAGCAG
    CAGGAAAAGA ACTTGGTGAA TGGGGCGAAG AGCCAGCCTG AAGTGAAGTG CAACATGGAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821489.2
    CDS30..2852
    Translation

    Target ORF information:

    RefSeq Version XM_012966035.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphodiesterase 5A (pde5a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966035.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCGCCTCT CACTTCCGTC TGCTGCTGCT GGGGTGGATC CGAAGGGCAA TCACCACACA 
    TATGCATTTA GGAAGTTGTG GAAAACCTCC TCACTCGAGA GCTGCCTTTT ATTTATACAT
    TCTTCTTATG TCCCCCCTCC CTGTGCGCTA TTCTTTCGCC GGTTCCCTTC CCCGGCCTGT
    CTTGGAAAGA GAATGTGGCA GGGGAATCTT TACCGCGAAG CCTCAGTGGC CGCTGTTGGT
    TTCCCTCTGG CTGCTGCCCC GGAGATGGGG GACACCCCCG AACCCCAAGT TGAAAACTGG
    GCTGCTACCC ATTCGGCTAC AGTGAACTCC AGCTGCAATG CGCATGCGTG CTGCACTCTA
    CAAGAAATGG TCAACGCATG GTTTGCTGAA CGAGTTCATA CAATACCAGC TAGTAAAGAG
    CAAGGAAAAA ATAATTCAGA GTCTAATCCT CAGGTGTGCT ATTCAGAAAA TACCACTCAG
    GGAACTCCAG TGAGGAAGAT CTCAGCCTCA GAGTTCGATA GGCCCCTAAG GCCAATTGTT
    GTCAAAGATT CTGTCGGAAC ACTGAAATTT CTTTCTGATT CTGAAAAGAA GGAACTGATG
    CCTCTTCAAC CACCCAGTCT GTCCAGAGCA GGAGATCAGT GCTCAAGGCT TTTGGAACTG
    GTGAAAGATA TTTCAAGTCA CCTTGACGTC ACAACACTTT GTCATAAAAT TTTTTTGCAC
    ATCCATGAGC TAATAGCAGC AGATCGTTAT TCGCTCTTTT TGGTTTGTGA GGACAGTTCC
    AATAAGAAGT TTCTGGTCAG TAGACTGTTT GACGTGGCAG AAGGTACAAC TGTGGAACAG
    GCTTCAAACA ATTGCATCCG TTTGGAATGG AATAAAGGGA TTGTTGGTCA CGTGGCAGAG
    TTTGGTCAGC CCTTGAATAT AAAGGATGCT TACCAGGACT CCAGATTCAA TGCGGAAGTC
    GACCAAATTA CTGGGTACAA AACACAAAGT ATTCTTTGTA TGCCAATTAA GAACCACAGG
    GAAGAGGTGG TTGGTGTAGC ACAAGCAATC AATAAGAAGT CAGGAAGCAA TGGGACATTT
    ACTGACCAAG ATGAAAAGGA CTTTGCTGCT TATCTGGCAT TCTGCGGAAT TGTTCTACAC
    AATGCACAAC TTTACGAAAC ATCACTGCTT GAAAACAGGA GGAACCAGGT GCTGCTTGAC
    CTTGCAACCT TGATATTTGA AGAACAGCAA TCACTGGAAG TCATCTTAAA GAAGATCGCA
    GCTACCATTC TGTCATTTAT GCAGGCTCAG AGATGCACAA TATTCATTGT GGATGAAGAT
    AGTCCTGATA CCTTTTCAAG TGTGTTTCAT ATGGAGTCTG ATGAATTAGA AGAGTCTGCA
    GATGCTGTAA AAAGGGAATA TGATGTGAGC AAAATGAATT ACATGTATGC ACAGTATGTA
    GCAAATACAA TGGAAGCAAT TAACATTCCA GACATATGTA AGGACACAAG ATTTCCATGG
    ACAAACGGAA ATGAAGAGAA GAATTTCAAG CATATTCAGA GTTTGCTTTG CTCCCCAATC
    AAGAATGCAA AGAAGAATAA AGTAATAGGA GTCTGTCAAC TTGTAAATAA GGTGGATGAA
    AACTCTGGCA AAATTAAAGC ATTCAACAAA AACGATGAAC AGTTTCTAGA AGCCTTTGCC
    ATATTCTGTG GCTTGGGAAT TCAGAACACC CAGATGTTTG AAGTTGTGGA GCGTGCGATG
    GCCAAACAGA TGGTGACATT AGAGATTCTT TCATATCACG CATCTGCTGC TGAGGAAGAA
    ACAATGGAGC TGCAGGGAGC CATTGTGCCA TCTGCACAAT CGCTTAAACT GACAGAATTC
    TACTTCAGCG ATTTTGAGCT TTCTGACATG GAAACCACAT TAGCCACAAG CCGAATGTTC
    ACAGACCTCA ACCTTGTGCA GACCTTCCAA ATGAAATATG AGACCCTTTG CCGGTGGATC
    CTGAGCGTGA AGAAGAACTA TAGAAAAAAT GTTGCATATC ACAACTGGAG ACATGCCTTT
    AATACAGCCC AGTGTATGTT CGCAGCCCTG AGAACTGGCA AAATTCAGGG TAAGCTAAAT
    GACCTAGAAA TCCTTGCACT GATGATAGCC ACCTTGAGCC ATGACTTGGA CCACAGAGGA
    GTTAACAATT CTTTTATACA GAGAAGTGAA CATCCTCTAG CACAGCTATA CTGTCATTCT
    ATCATGGAGA ACCACCATTT TGACCAGTGC CTAATGATAC TAAACAGTCA GGGTAACCAG
    ATTCTAAGTG GGCTTTCTGT TCAAGACTAC AAGACTGTTC TGAAGATGAT AAAACAAGCC
    ATCCTTGCTA CTGATTTGGC ACTATACATT AAGCGAAGAA GTGATTTCTT TGAATTAGTG
    AATAAGAAGA AATTCAAATG GGATGATCCT TCACAAAAAG AACTGTTCTT AGCAATGCTG
    ATGACGGCTT GTGATCTGTC TGCAATAACA AAGCCATGGC CAGTTCAACA ACGGATAGCT
    GAACTTGTAG CCTCAGAGTT TTATGACCAA GGGGATAAAG AGAGGAGGGA ACTTAATATT
    GAACCAATAG ACCTGATGAA CAGAGAAAAG AAAGACAAAA TACCCAGCAT GCAGGTTGGA
    TTTATTGACG CAGTCTGCTT AGAACTTTAT GAGGCCTTAA CGCAGATCTC TGAATCGTGT
    TACCCTTTAC TTGATGGCTG TCAGAGAAAT AGGCAGATGT GGCAGATCTT AGCAGAGCAG
    CAGGAAAAGA ACTTGGTGAA TGGGGCGAAG AGCCAGCCTG AAGTGAAGTG CAACATGGAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48075
    Clone ID Related Accession (Same CDS sequence) XM_012966045.3
    Accession Version XM_012966045.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGGGAA GCTGCAGCCT GCAAAGCCAG GAGCAGGAGG GCATAGAGTC GTGGCTGGAC 
    GATCACTGGG ACTTCACACA CTCGTACTTT GTGAGGAAAG CCACAAGAGA AATGGTCAAC
    GCATGGTTTG CTGAACGAGT TCATACAATA CCAGCTAGTA AAGAGCAAGG AAAAAATAAT
    TCAGAGTCTA ATCCTCAGGT GTGCTATTCA GAAAATACCA CTCAGGGAAC TCCAGTGAGG
    AAGATCTCAG CCTCAGAGTT CGATAGGCCC CTAAGGCCAA TTGTTGTCAA AGATTCTGTC
    GGAACACTGA AATTTCTTTC TGATTCTGAA AAGAAGGAAC TGATGCCTCT TCAACCACCC
    AGTCTGTCCA GAGCAGGAGA TCAGTGCTCA AGGCTTTTGG AACTGGTGAA AGATATTTCA
    AGTCACCTTG ACGTCACAAC ACTTTGTCAT AAAATTTTTT TGCACATCCA TGAGCTAATA
    GCAGCAGATC GTTATTCGCT CTTTTTGGTT TGTGAGGACA GTTCCAATAA GAAGTTTCTG
    GTCAGTAGAC TGTTTGACGT GGCAGAAGGT ACAACTGTGG AACAGGCTTC AAACAATTGC
    ATCCGTTTGG AATGGAATAA AGGGATTGTT GGTCACGTGG CAGAGTTTGG TCAGCCCTTG
    AATATAAAGG ATGCTTACCA GGACTCCAGA TTCAATGCGG AAGTCGACCA AATTACTGGG
    TACAAAACAC AAAGTATTCT TTGTATGCCA ATTAAGAACC ACAGGGAAGA GGTGGTTGGT
    GTAGCACAAG CAATCAATAA GAAGTCAGGA AGCAATGGGA CATTTACTGA CCAAGATGAA
    AAGGACTTTG CTGCTTATCT GGCATTCTGC GGAATTGTTC TACACAATGC ACAACTTTAC
    GAAACATCAC TGCTTGAAAA CAGGAGGAAC CAGGTGCTGC TTGACCTTGC AACCTTGATA
    TTTGAAGAAC AGCAATCACT GGAAGTCATC TTAAAGAAGA TCGCAGCTAC CATTCTGTCA
    TTTATGCAGG CTCAGAGATG CACAATATTC ATTGTGGATG AAGATAGTCC TGATACCTTT
    TCAAGTGTGT TTCATATGGA GTCTGATGAA TTAGAAGAGT CTGCAGATGC TGTAAAAAGG
    GAATATGATG TGAGCAAAAT GAATTACATG TATGCACAGT ATGTAGCAAA TACAATGGAA
    GCAATTAACA TTCCAGACAT ATGTAAGGAC ACAAGATTTC CATGGACAAA CGGAAATGAA
    GAGAAGAATT TCAAGCATAT TCAGAGTTTG CTTTGCTCCC CAATCAAGAA TGCAAAGAAG
    AATAAAGTAA TAGGAGTCTG TCAACTTGTA AATAAGGTGG ATGAAAACTC TGGCAAAATT
    AAAGCATTCA ACAAAAACGA TGAACAGTTT CTAGAAGCCT TTGCCATATT CTGTGGCTTG
    GGAATTCAGA ACACCCAGAT GTTTGAAGTT GTGGAGCGTG CGATGGCCAA ACAGATGGTG
    ACATTAGAGA TTCTTTCATA TCACGCATCT GCTGCTGAGG AAGAAACAAT GGAGCTGCAG
    GTAACAGCGG GAGCCATTGT GCCATCTGCA CAATCGCTTA AACTGACAGA ATTCTACTTC
    AGCGATTTTG AGCTTTCTGA CATGGAAACC ACATTAGCCA CAAGCCGAAT GTTCACAGAC
    CTCAACCTTG TGCAGACCTT CCAAATGAAA TATGAGACCC TTTGCCGGTG GATCCTGAGC
    GTGAAGAAGA ACTATAGAAA AAATGTTGCA TATCACAACT GGAGACATGC CTTTAATACA
    GCCCAGTGTA TGTTCGCAGC CCTGAGAACT GGCAAAATTC AGGGTAAGCT AAATGACCTA
    GAAATCCTTG CACTGATGAT AGCCACCTTG AGCCATGACT TGGACCACAG AGGAGTTAAC
    AATTCTTTTA TACAGAGAAG TGAACATCCT CTAGCACAGC TATACTGTCA TTCTATCATG
    GAGAACCACC ATTTTGACCA GTGCCTAATG ATACTAAACA GTCAGGGTAA CCAGATTCTA
    AGTGGGCTTT CTGTTCAAGA CTACAAGACT GTTCTGAAGA TGATAAAACA AGCCATCCTT
    GCTACTGATT TGGCACTATA CATTAAGCGA AGAAGTGATT TCTTTGAATT AGTGAATAAG
    AAGAAATTCA AATGGGATGA TCCTTCACAA AAAGAACTGT TCTTAGCAAT GCTGATGACG
    GCTTGTGATC TGTCTGCAAT AACAAAGCCA TGGCCAGTTC AACAACGGAT AGCTGAACTT
    GTAGCCTCAG AGTTTTATGA CCAAGGGGAT AAAGAGAGGA GGGAACTTAA TATTGAACCA
    ATAGACCTGA TGAACAGAGA AAAGAAAGAC AAAATACCCA GCATGCAGGT TGGATTTATT
    GACGCAGTCT GCTTAGAACT TTATGAGGCC TTAACGCAGA TCTCTGAATC GTGTTACCCT
    TTACTTGATG GCTGTCAGAG AAATAGGCAG ATGTGGCAGA TCTTAGCAGA GCAGCAGGAA
    AAGAACTTGG TGAATGGGGC GAAGAGCCAG CCTGAAGTGA AGTGCAACAT GGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821499.1
    CDS369..2945
    Translation

    Target ORF information:

    RefSeq Version XM_012966045.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphodiesterase 5A (pde5a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966045.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGGGAA GCTGCAGCCT GCAAAGCCAG GAGCAGGAGG GCATAGAGTC GTGGCTGGAC 
    GATCACTGGG ACTTCACACA CTCGTACTTT GTGAGGAAAG CCACAAGAGA AATGGTCAAC
    GCATGGTTTG CTGAACGAGT TCATACAATA CCAGCTAGTA AAGAGCAAGG AAAAAATAAT
    TCAGAGTCTA ATCCTCAGGT GTGCTATTCA GAAAATACCA CTCAGGGAAC TCCAGTGAGG
    AAGATCTCAG CCTCAGAGTT CGATAGGCCC CTAAGGCCAA TTGTTGTCAA AGATTCTGTC
    GGAACACTGA AATTTCTTTC TGATTCTGAA AAGAAGGAAC TGATGCCTCT TCAACCACCC
    AGTCTGTCCA GAGCAGGAGA TCAGTGCTCA AGGCTTTTGG AACTGGTGAA AGATATTTCA
    AGTCACCTTG ACGTCACAAC ACTTTGTCAT AAAATTTTTT TGCACATCCA TGAGCTAATA
    GCAGCAGATC GTTATTCGCT CTTTTTGGTT TGTGAGGACA GTTCCAATAA GAAGTTTCTG
    GTCAGTAGAC TGTTTGACGT GGCAGAAGGT ACAACTGTGG AACAGGCTTC AAACAATTGC
    ATCCGTTTGG AATGGAATAA AGGGATTGTT GGTCACGTGG CAGAGTTTGG TCAGCCCTTG
    AATATAAAGG ATGCTTACCA GGACTCCAGA TTCAATGCGG AAGTCGACCA AATTACTGGG
    TACAAAACAC AAAGTATTCT TTGTATGCCA ATTAAGAACC ACAGGGAAGA GGTGGTTGGT
    GTAGCACAAG CAATCAATAA GAAGTCAGGA AGCAATGGGA CATTTACTGA CCAAGATGAA
    AAGGACTTTG CTGCTTATCT GGCATTCTGC GGAATTGTTC TACACAATGC ACAACTTTAC
    GAAACATCAC TGCTTGAAAA CAGGAGGAAC CAGGTGCTGC TTGACCTTGC AACCTTGATA
    TTTGAAGAAC AGCAATCACT GGAAGTCATC TTAAAGAAGA TCGCAGCTAC CATTCTGTCA
    TTTATGCAGG CTCAGAGATG CACAATATTC ATTGTGGATG AAGATAGTCC TGATACCTTT
    TCAAGTGTGT TTCATATGGA GTCTGATGAA TTAGAAGAGT CTGCAGATGC TGTAAAAAGG
    GAATATGATG TGAGCAAAAT GAATTACATG TATGCACAGT ATGTAGCAAA TACAATGGAA
    GCAATTAACA TTCCAGACAT ATGTAAGGAC ACAAGATTTC CATGGACAAA CGGAAATGAA
    GAGAAGAATT TCAAGCATAT TCAGAGTTTG CTTTGCTCCC CAATCAAGAA TGCAAAGAAG
    AATAAAGTAA TAGGAGTCTG TCAACTTGTA AATAAGGTGG ATGAAAACTC TGGCAAAATT
    AAAGCATTCA ACAAAAACGA TGAACAGTTT CTAGAAGCCT TTGCCATATT CTGTGGCTTG
    GGAATTCAGA ACACCCAGAT GTTTGAAGTT GTGGAGCGTG CGATGGCCAA ACAGATGGTG
    ACATTAGAGA TTCTTTCATA TCACGCATCT GCTGCTGAGG AAGAAACAAT GGAGCTGCAG
    GTAACAGCGG GAGCCATTGT GCCATCTGCA CAATCGCTTA AACTGACAGA ATTCTACTTC
    AGCGATTTTG AGCTTTCTGA CATGGAAACC ACATTAGCCA CAAGCCGAAT GTTCACAGAC
    CTCAACCTTG TGCAGACCTT CCAAATGAAA TATGAGACCC TTTGCCGGTG GATCCTGAGC
    GTGAAGAAGA ACTATAGAAA AAATGTTGCA TATCACAACT GGAGACATGC CTTTAATACA
    GCCCAGTGTA TGTTCGCAGC CCTGAGAACT GGCAAAATTC AGGGTAAGCT AAATGACCTA
    GAAATCCTTG CACTGATGAT AGCCACCTTG AGCCATGACT TGGACCACAG AGGAGTTAAC
    AATTCTTTTA TACAGAGAAG TGAACATCCT CTAGCACAGC TATACTGTCA TTCTATCATG
    GAGAACCACC ATTTTGACCA GTGCCTAATG ATACTAAACA GTCAGGGTAA CCAGATTCTA
    AGTGGGCTTT CTGTTCAAGA CTACAAGACT GTTCTGAAGA TGATAAAACA AGCCATCCTT
    GCTACTGATT TGGCACTATA CATTAAGCGA AGAAGTGATT TCTTTGAATT AGTGAATAAG
    AAGAAATTCA AATGGGATGA TCCTTCACAA AAAGAACTGT TCTTAGCAAT GCTGATGACG
    GCTTGTGATC TGTCTGCAAT AACAAAGCCA TGGCCAGTTC AACAACGGAT AGCTGAACTT
    GTAGCCTCAG AGTTTTATGA CCAAGGGGAT AAAGAGAGGA GGGAACTTAA TATTGAACCA
    ATAGACCTGA TGAACAGAGA AAAGAAAGAC AAAATACCCA GCATGCAGGT TGGATTTATT
    GACGCAGTCT GCTTAGAACT TTATGAGGCC TTAACGCAGA TCTCTGAATC GTGTTACCCT
    TTACTTGATG GCTGTCAGAG AAATAGGCAG ATGTGGCAGA TCTTAGCAGA GCAGCAGGAA
    AAGAACTTGG TGAATGGGGC GAAGAGCCAG CCTGAAGTGA AGTGCAACAT GGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48076
    Clone ID Related Accession (Same CDS sequence) XM_031902313.1
    Accession Version XM_031902313.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTCAACG CATGGTTTGC TGAACGAGTT CATACAATAC CAGCTAGTAA AGAGCAAGGA 
    AAAAATAATT CAGAGTCTAA TCCTCAGGTG TGCTATTCAG AAAATACCAC TCAGGGAACT
    CCAGTGAGGA AGATCTCAGC CTCAGAGTTC GATAGGCCCC TAAGGCCAAT TGTTGTCAAA
    GATTCTGTCG GAACACTGAA ATTTCTTTCT GATTCTGAAA AGAAGGAACT GATGCCTCTT
    CAACCACCCA GTCTGTCCAG AGCAGGAGAT CAGTGCTCAA GGCTTTTGGA ACTGGTGAAA
    GATATTTCAA GTCACCTTGA CGTCACAACA CTTTGTCATA AAATTTTTTT GCACATCCAT
    GAGCTAATAG CAGCAGATCG TTATTCGCTC TTTTTGGTTT GTGAGGACAG TTCCAATAAG
    AAGTTTCTGG TCAGTAGACT GTTTGACGTG GCAGAAGGTA CAACTGTGGA ACAGGCTTCA
    AACAATTGCA TCCGTTTGGA ATGGAATAAA GGGATTGTTG GTCACGTGGC AGAGTTTGGT
    CAGCCCTTGA ATATAAAGGA TGCTTACCAG GACTCCAGAT TCAATGCGGA AGTCGACCAA
    ATTACTGGGT ACAAAACACA AAGTATTCTT TGTATGCCAA TTAAGAACCA CAGGGAAGAG
    GTGGTTGGTG TAGCACAAGC AATCAATAAG AAGTCAGGAA GCAATGGGAC ATTTACTGAC
    CAAGATGAAA AGGACTTTGC TGCTTATCTG GCATTCTGCG GAATTGTTCT ACACAATGCA
    CAACTTTACG AAACATCACT GCTTGAAAAC AGGAGGAACC AGGTGCTGCT TGACCTTGCA
    ACCTTGATAT TTGAAGAACA GCAATCACTG GAAGTCATCT TAAAGAAGAT CGCAGCTACC
    ATTCTGTCAT TTATGCAGGC TCAGAGATGC ACAATATTCA TTGTGGATGA AGATAGTCCT
    GATACCTTTT CAAGTGTGTT TCATATGGAG TCTGATGAAT TAGAAGAGTC TGCAGATGCT
    GTAAAAAGGG AATATGATGT GAGCAAAATG AATTACATGT ATGCACAGTA TGTAGCAAAT
    ACAATGGAAG CAATTAACAT TCCAGACATA TGTAAGGACA CAAGATTTCC ATGGACAAAC
    GGAAATGAAG AGAAGAATTT CAAGCATATT CAGAGTTTGC TTTGCTCCCC AATCAAGAAT
    GCAAAGAAGA ATAAAGTAAT AGGAGTCTGT CAACTTGTAA ATAAGGTGGA TGAAAACTCT
    GGCAAAATTA AAGCATTCAA CAAAAACGAT GAACAGTTTC TAGAAGCCTT TGCCATATTC
    TGTGGCTTGG GAATTCAGAA CACCCAGATG TTTGAAGTTG TGGAGCGTGC GATGGCCAAA
    CAGATGGTGA CATTAGAGAT TCTTTCATAT CACGCATCTG CTGCTGAGGA AGAAACAATG
    GAGCTGCAGG TAACAGCGGG AGCCATTGTG CCATCTGCAC AATCGCTTAA ACTGACAGAA
    TTCTACTTCA GCGATTTTGA GCTTTCTGAC ATGGAAACCA CATTAGCCAC AAGCCGAATG
    TTCACAGACC TCAACCTTGT GCAGACCTTC CAAATGAAAT ATGAGACCCT TTGCCGGTGG
    ATCCTGAGCG TGAAGAAGAA CTATAGAAAA AATGTTGCAT ATCACAACTG GAGACATGCC
    TTTAATACAG CCCAGTGTAT GTTCGCAGCC CTGAGAACTG GCAAAATTCA GGGTAAGCTA
    AATGACCTAG AAATCCTTGC ACTGATGATA GCCACCTTGA GCCATGACTT GGACCACAGA
    GGAGTTAACA ATTCTTTTAT ACAGAGAAGT GAACATCCTC TAGCACAGCT ATACTGTCAT
    TCTATCATGG AGAACCACCA TTTTGACCAG TGCCTAATGA TACTAAACAG TCAGGGTAAC
    CAGATTCTAA GTGGGCTTTC TGTTCAAGAC TACAAGACTG TTCTGAAGAT GATAAAACAA
    GCCATCCTTG CTACTGATTT GGCACTATAC ATTAAGCGAA GAAGTGATTT CTTTGAATTA
    GTGAATAAGA AGAAATTCAA ATGGGATGAT CCTTCACAAA AAGAACTGTT CTTAGCAATG
    CTGATGACGG CTTGTGATCT GTCTGCAATA ACAAAGCCAT GGCCAGTTCA ACAACGGATA
    GCTGAACTTG TAGCCTCAGA GTTTTATGAC CAAGGGGATA AAGAGAGGAG GGAACTTAAT
    ATTGAACCAA TAGACCTGAT GAACAGAGAA AAGAAAGACA AAATACCCAG CATGCAGGTT
    GGATTTATTG ACGCAGTCTG CTTAGAACTT TATGAGGCCT TAACGCAGAT CTCTGAATCG
    TGTTACCCTT TACTTGATGG CTGTCAGAGA AATAGGCAGA TGTGGCAGAT CTTAGCAGAG
    CAGCAGGAAA AGAACTTGGT GAATGGGGCG AAGAGCCAGC CTGAAGTGAA GTGCAACATG
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758173.1
    CDS314..2779
    Translation

    Target ORF information:

    RefSeq Version XM_031902313.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphodiesterase 5A (pde5a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTCAACG CATGGTTTGC TGAACGAGTT CATACAATAC CAGCTAGTAA AGAGCAAGGA 
    AAAAATAATT CAGAGTCTAA TCCTCAGGTG TGCTATTCAG AAAATACCAC TCAGGGAACT
    CCAGTGAGGA AGATCTCAGC CTCAGAGTTC GATAGGCCCC TAAGGCCAAT TGTTGTCAAA
    GATTCTGTCG GAACACTGAA ATTTCTTTCT GATTCTGAAA AGAAGGAACT GATGCCTCTT
    CAACCACCCA GTCTGTCCAG AGCAGGAGAT CAGTGCTCAA GGCTTTTGGA ACTGGTGAAA
    GATATTTCAA GTCACCTTGA CGTCACAACA CTTTGTCATA AAATTTTTTT GCACATCCAT
    GAGCTAATAG CAGCAGATCG TTATTCGCTC TTTTTGGTTT GTGAGGACAG TTCCAATAAG
    AAGTTTCTGG TCAGTAGACT GTTTGACGTG GCAGAAGGTA CAACTGTGGA ACAGGCTTCA
    AACAATTGCA TCCGTTTGGA ATGGAATAAA GGGATTGTTG GTCACGTGGC AGAGTTTGGT
    CAGCCCTTGA ATATAAAGGA TGCTTACCAG GACTCCAGAT TCAATGCGGA AGTCGACCAA
    ATTACTGGGT ACAAAACACA AAGTATTCTT TGTATGCCAA TTAAGAACCA CAGGGAAGAG
    GTGGTTGGTG TAGCACAAGC AATCAATAAG AAGTCAGGAA GCAATGGGAC ATTTACTGAC
    CAAGATGAAA AGGACTTTGC TGCTTATCTG GCATTCTGCG GAATTGTTCT ACACAATGCA
    CAACTTTACG AAACATCACT GCTTGAAAAC AGGAGGAACC AGGTGCTGCT TGACCTTGCA
    ACCTTGATAT TTGAAGAACA GCAATCACTG GAAGTCATCT TAAAGAAGAT CGCAGCTACC
    ATTCTGTCAT TTATGCAGGC TCAGAGATGC ACAATATTCA TTGTGGATGA AGATAGTCCT
    GATACCTTTT CAAGTGTGTT TCATATGGAG TCTGATGAAT TAGAAGAGTC TGCAGATGCT
    GTAAAAAGGG AATATGATGT GAGCAAAATG AATTACATGT ATGCACAGTA TGTAGCAAAT
    ACAATGGAAG CAATTAACAT TCCAGACATA TGTAAGGACA CAAGATTTCC ATGGACAAAC
    GGAAATGAAG AGAAGAATTT CAAGCATATT CAGAGTTTGC TTTGCTCCCC AATCAAGAAT
    GCAAAGAAGA ATAAAGTAAT AGGAGTCTGT CAACTTGTAA ATAAGGTGGA TGAAAACTCT
    GGCAAAATTA AAGCATTCAA CAAAAACGAT GAACAGTTTC TAGAAGCCTT TGCCATATTC
    TGTGGCTTGG GAATTCAGAA CACCCAGATG TTTGAAGTTG TGGAGCGTGC GATGGCCAAA
    CAGATGGTGA CATTAGAGAT TCTTTCATAT CACGCATCTG CTGCTGAGGA AGAAACAATG
    GAGCTGCAGG TAACAGCGGG AGCCATTGTG CCATCTGCAC AATCGCTTAA ACTGACAGAA
    TTCTACTTCA GCGATTTTGA GCTTTCTGAC ATGGAAACCA CATTAGCCAC AAGCCGAATG
    TTCACAGACC TCAACCTTGT GCAGACCTTC CAAATGAAAT ATGAGACCCT TTGCCGGTGG
    ATCCTGAGCG TGAAGAAGAA CTATAGAAAA AATGTTGCAT ATCACAACTG GAGACATGCC
    TTTAATACAG CCCAGTGTAT GTTCGCAGCC CTGAGAACTG GCAAAATTCA GGGTAAGCTA
    AATGACCTAG AAATCCTTGC ACTGATGATA GCCACCTTGA GCCATGACTT GGACCACAGA
    GGAGTTAACA ATTCTTTTAT ACAGAGAAGT GAACATCCTC TAGCACAGCT ATACTGTCAT
    TCTATCATGG AGAACCACCA TTTTGACCAG TGCCTAATGA TACTAAACAG TCAGGGTAAC
    CAGATTCTAA GTGGGCTTTC TGTTCAAGAC TACAAGACTG TTCTGAAGAT GATAAAACAA
    GCCATCCTTG CTACTGATTT GGCACTATAC ATTAAGCGAA GAAGTGATTT CTTTGAATTA
    GTGAATAAGA AGAAATTCAA ATGGGATGAT CCTTCACAAA AAGAACTGTT CTTAGCAATG
    CTGATGACGG CTTGTGATCT GTCTGCAATA ACAAAGCCAT GGCCAGTTCA ACAACGGATA
    GCTGAACTTG TAGCCTCAGA GTTTTATGAC CAAGGGGATA AAGAGAGGAG GGAACTTAAT
    ATTGAACCAA TAGACCTGAT GAACAGAGAA AAGAAAGACA AAATACCCAG CATGCAGGTT
    GGATTTATTG ACGCAGTCTG CTTAGAACTT TATGAGGCCT TAACGCAGAT CTCTGAATCG
    TGTTACCCTT TACTTGATGG CTGTCAGAGA AATAGGCAGA TGTGGCAGAT CTTAGCAGAG
    CAGCAGGAAA AGAACTTGGT GAATGGGGCG AAGAGCCAGC CTGAAGTGAA GTGCAACATG
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.