PDE5A cDNA ORF clone, Gallus gallus(chicken)

The following PDE5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa08193 XM_420631.6
Latest version!
Gallus gallus phosphodiesterase 5A (PDE5A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OGa08194 XM_004941236.3
Latest version!
Gallus gallus phosphodiesterase 5A (PDE5A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OGa08195 XM_004941237.3
Latest version!
Gallus gallus phosphodiesterase 5A (PDE5A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OGa08196 XM_004941238.3
Latest version!
Gallus gallus phosphodiesterase 5A (PDE5A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OGa08197 XM_004941239.3
Latest version!
Gallus gallus phosphodiesterase 5A (PDE5A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OGa62936 NM_001319020.1
Latest version!
Gallus gallus phosphodiesterase 5A (PDE5A), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa35174 XM_015276368.2
Latest version!
Gallus gallus phosphodiesterase 5A (PDE5A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa35174 XM_025149655.1
Latest version!
Gallus gallus phosphodiesterase 5A (PDE5A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OGa08194 XM_004941236.2 Gallus gallus phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OGa08193 XM_420631.5 Gallus gallus phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OGa08196 XM_004941238.2 Gallus gallus phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OGa08195 XM_004941237.2 Gallus gallus phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OGa35174 XM_015276368.1 Gallus gallus phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa08197 XM_004941239.2 Gallus gallus phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa08193
    Clone ID Related Accession (Same CDS sequence) XM_420631.5 , XM_420631.6
    Accession Version XM_420631.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_420631.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGCA GCTGCCACCC CTCGCCTGAG CAGGAAGCGG TGGAAGCGTG GCTGGACGAT 
    CACTGCGACT TCACCCGCTC CTACTTTGTC AGGAAAGCCA CAAGAGAAAT GGTTAATGCA
    TGGTTTGCTG AGAGAGTTCA TACCATCCCT GTCTGCAAGG AGGGGACCAA GTCTCAGAGT
    GAATCCTGTG CTTGTCAGCA GCTTGCCTGC TCTGAGAACA CCAACTCTGG AACACCAGCA
    AGGAAAATCT CTGCTTCAGA ATTTGACAGA CCCTTAAGGC CGATTTTTGT CAAGGACTCA
    GTAGGAGCAG TAAGCTTCCT CTCTGGCTCT GAAAAGAAGG AACAGATGCC TCTGCAATCT
    CCAAGGACTG GGACCAGTGC AGGGGATCGG TGCTCAAGGC TTTTAGAACT TGTGAAAGAC
    ATTTCTAGTC ATTTGGATGT CACAGCACTT TGCCATAAAA TTTTCCTGCA TATCCATGAG
    CTTATAGCTG CTGATCGCTA TTCCCTCTTC TTGGTCTGTG AGGATAGCTC TAACGAGAAG
    TTTCTTGTAA GCAGGCTGTT TGATGTGGCT GAAGGTTCCA CCCTAGAAGA AGCTTCAAAT
    AACTGCATTC GCCTGGAGTG GAACAAGGGC ATCGTGGGCC ACGTAGCTGC TATAGGCCAG
    CCTCTGAACA TCAAGAATGC GTATGAGGAT CCAAGATTCA ATGCAGAAGT TGACCAGATC
    ACAGGGTATA AGACTCAGAG CATTCTCTGT ATGCCAATAA AGAATCATAG GGAAGAGGTT
    GTTGGTGTGG CTCAAGCAAT TAATAAGAAA TCTGGAACTG GTGGGACTTT CACGGAACAA
    GATGAAAAGG ATTTTGCTGC ATATTTGGCA TTTTGTGGAA TTGTTCTTCA CAACGCTCAA
    TTGTATGAAA CATCTTTGCT TGAAAACAGG CGTAATCAGG TGCTTCTTGA TCTTGCCAGC
    CTTATTTTTG AAGAACAACA GTCTTTGGAA GTAATTCTGA AGAAGATAGC TGCCACTATA
    ATATCCTTCA TGCAAGTGCA GAGGTGTACC ATCTTCATAG TGGACGAAGA TTGTACTGAT
    TCATTTTCTA GTGTTTTTCA TATGGAAGCT GAGGAATTAG AAGATTCAGC AGAAAACCAG
    AAGAGGGACT GTGATACAAA CAAAATCAAT TATATGTATG CTCAGTATGT CAAGAATACA
    ATGGAGCCTC TCAACATCCC AGATGTCTGC AAAGACAGAA GATTTCCCTG GACAACTGAC
    AGTGCAGAAA GTGTAAGTCA ACATATAAAG AGTTTGCTGT GTACACCAAT AAAAAATGGG
    AAAAAGAATA AGGTGATAGG GGTTTGCCAG CTTGTGAATA AGATGGAAGA AAACTCAGGC
    AAGATCAAGG CTTTCAACAG AAATGATGAG CAGTTTCTGG AAGCATTTGT CATCTTTTGT
    GGCTTGGGGA TCCAGAATAC ACAGATGTAT GAAGCTGTAG AAAGAGCAAT GGCCAAACAG
    ATGGTGACAT TAGAGGTTCT CTCATACCAT GCTTCTGCTG CTGAGGAGGA ATCAAGGGAG
    CTGCAGGTAA CAGCGGCTGC TGTAGTACCA TCTGCACAAT CTCTCAACCT AACTGACTTC
    AACTTCAGTG ATTTTGAGCT ATCAGATTTT GAAACAACAC TGTGTACAAT TCGAATGTTC
    ACAGACCTCA ACCTGGTGCA GAATTTCCAA ATGAAACATG AGGTTCTCTG CAGATGGATT
    TTAAGCGTGA AGAAAAATTA TCGGAAAAAT GTTGCTTATC ACAATTGGAG ACATGCCTTT
    AATACAGCAC AGTGCATGTT TGCTGCTTTA AAATCTGGTA AAATTCAGAG CAAACTGACT
    GACTTGGAAA CGCTGGCTTT GCTGATAGCA ACTCTAAGCC ATGATTTGGA TCACAGGGGA
    GTGAACAACT CTTACATACA AAGAAGTGAA CATCCACTGG CTCAACTGTA TTGCCACTCC
    ATCATGGAGC ATCACCATTT TGATCAATGC CTGATGATCC TGAATAGTCC AGGAAACCAG
    ATTCTCAGCA GCCTTTCAAT TGAAGAGTAC AAGGCAACAC TGAAAATGAT AAAACAAGCC
    ATCCTTGCAA CAGACCTAGC ACTATACATA AAGAGGAGAG GAGAATTTTT TGAACTTCTA
    AGGAAAAAGC AGTTTAATTG GGAAGATCCT ATGCAGAAGG AGCTATTTTT AGCAATGCTG
    ATGACTGCTT GTGATTTATC AGCTATAACC AAACCGTGGC CAGTTCAGCA ACGGATTGCT
    GAGCTTGTAG CTACTGAGTT CTTTGATCAG GGAGATAAAG AGCGGAAGGA ACTCAACATA
    GAACCAACAG ATCTAATGAA CAGAGAGAAG AAAAACAAAA TTCCAAGCAT GCAGGTTGGA
    TTCATTGATG CTGTTTGCTT GCAGCTGTAT GAGGCCCTAA CACATGTATC AGAGGCCTGC
    TACCCTTTAC TGGATGGCTG TAGAAAAAAC AGGCAGAAAT GGCAGTCCTT AGCTGAACAA
    CAAGAGAAGA ATCTGATTAA TGGAGAAAGC AATCAAGCCA AACGGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_420631.3
    CDS1766..4336
    Translation

    Target ORF information:

    RefSeq Version XM_420631.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_420631.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGCA GCTGCCACCC CTCGCCTGAG CAGGAAGCGG TGGAAGCGTG GCTGGACGAT 
    CACTGCGACT TCACCCGCTC CTACTTTGTC AGGAAAGCCA CAAGAGAAAT GGTTAATGCA
    TGGTTTGCTG AGAGAGTTCA TACCATCCCT GTCTGCAAGG AGGGGACCAA GTCTCAGAGT
    GAATCCTGTG CTTGTCAGCA GCTTGCCTGC TCTGAGAACA CCAACTCTGG AACACCAGCA
    AGGAAAATCT CTGCTTCAGA ATTTGACAGA CCCTTAAGGC CGATTTTTGT CAAGGACTCA
    GTAGGAGCAG TAAGCTTCCT CTCTGGCTCT GAAAAGAAGG AACAGATGCC TCTGCAATCT
    CCAAGGACTG GGACCAGTGC AGGGGATCGG TGCTCAAGGC TTTTAGAACT TGTGAAAGAC
    ATTTCTAGTC ATTTGGATGT CACAGCACTT TGCCATAAAA TTTTCCTGCA TATCCATGAG
    CTTATAGCTG CTGATCGCTA TTCCCTCTTC TTGGTCTGTG AGGATAGCTC TAACGAGAAG
    TTTCTTGTAA GCAGGCTGTT TGATGTGGCT GAAGGTTCCA CCCTAGAAGA AGCTTCAAAT
    AACTGCATTC GCCTGGAGTG GAACAAGGGC ATCGTGGGCC ACGTAGCTGC TATAGGCCAG
    CCTCTGAACA TCAAGAATGC GTATGAGGAT CCAAGATTCA ATGCAGAAGT TGACCAGATC
    ACAGGGTATA AGACTCAGAG CATTCTCTGT ATGCCAATAA AGAATCATAG GGAAGAGGTT
    GTTGGTGTGG CTCAAGCAAT TAATAAGAAA TCTGGAACTG GTGGGACTTT CACGGAACAA
    GATGAAAAGG ATTTTGCTGC ATATTTGGCA TTTTGTGGAA TTGTTCTTCA CAACGCTCAA
    TTGTATGAAA CATCTTTGCT TGAAAACAGG CGTAATCAGG TGCTTCTTGA TCTTGCCAGC
    CTTATTTTTG AAGAACAACA GTCTTTGGAA GTAATTCTGA AGAAGATAGC TGCCACTATA
    ATATCCTTCA TGCAAGTGCA GAGGTGTACC ATCTTCATAG TGGACGAAGA TTGTACTGAT
    TCATTTTCTA GTGTTTTTCA TATGGAAGCT GAGGAATTAG AAGATTCAGC AGAAAACCAG
    AAGAGGGACT GTGATACAAA CAAAATCAAT TATATGTATG CTCAGTATGT CAAGAATACA
    ATGGAGCCTC TCAACATCCC AGATGTCTGC AAAGACAGAA GATTTCCCTG GACAACTGAC
    AGTGCAGAAA GTGTAAGTCA ACATATAAAG AGTTTGCTGT GTACACCAAT AAAAAATGGG
    AAAAAGAATA AGGTGATAGG GGTTTGCCAG CTTGTGAATA AGATGGAAGA AAACTCAGGC
    AAGATCAAGG CTTTCAACAG AAATGATGAG CAGTTTCTGG AAGCATTTGT CATCTTTTGT
    GGCTTGGGGA TCCAGAATAC ACAGATGTAT GAAGCTGTAG AAAGAGCAAT GGCCAAACAG
    ATGGTGACAT TAGAGGTTCT CTCATACCAT GCTTCTGCTG CTGAGGAGGA ATCAAGGGAG
    CTGCAGGTAA CAGCGGCTGC TGTAGTACCA TCTGCACAAT CTCTCAACCT AACTGACTTC
    AACTTCAGTG ATTTTGAGCT ATCAGATTTT GAAACAACAC TGTGTACAAT TCGAATGTTC
    ACAGACCTCA ACCTGGTGCA GAATTTCCAA ATGAAACATG AGGTTCTCTG CAGATGGATT
    TTAAGCGTGA AGAAAAATTA TCGGAAAAAT GTTGCTTATC ACAATTGGAG ACATGCCTTT
    AATACAGCAC AGTGCATGTT TGCTGCTTTA AAATCTGGTA AAATTCAGAG CAAACTGACT
    GACTTGGAAA CGCTGGCTTT GCTGATAGCA ACTCTAAGCC ATGATTTGGA TCACAGGGGA
    GTGAACAACT CTTACATACA AAGAAGTGAA CATCCACTGG CTCAACTGTA TTGCCACTCC
    ATCATGGAGC ATCACCATTT TGATCAATGC CTGATGATCC TGAATAGTCC AGGAAACCAG
    ATTCTCAGCA GCCTTTCAAT TGAAGAGTAC AAGGCAACAC TGAAAATGAT AAAACAAGCC
    ATCCTTGCAA CAGACCTAGC ACTATACATA AAGAGGAGAG GAGAATTTTT TGAACTTCTA
    AGGAAAAAGC AGTTTAATTG GGAAGATCCT ATGCAGAAGG AGCTATTTTT AGCAATGCTG
    ATGACTGCTT GTGATTTATC AGCTATAACC AAACCGTGGC CAGTTCAGCA ACGGATTGCT
    GAGCTTGTAG CTACTGAGTT CTTTGATCAG GGAGATAAAG AGCGGAAGGA ACTCAACATA
    GAACCAACAG ATCTAATGAA CAGAGAGAAG AAAAACAAAA TTCCAAGCAT GCAGGTTGGA
    TTCATTGATG CTGTTTGCTT GCAGCTGTAT GAGGCCCTAA CACATGTATC AGAGGCCTGC
    TACCCTTTAC TGGATGGCTG TAGAAAAAAC AGGCAGAAAT GGCAGTCCTT AGCTGAACAA
    CAAGAGAAGA ATCTGATTAA TGGAGAAAGC AATCAAGCCA AACGGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa08193
    Clone ID Related Accession (Same CDS sequence) XM_420631.5 , XM_420631.6
    Accession Version XM_420631.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_420631.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGCA GCTGCCACCC CTCGCCTGAG CAGGAAGCGG TGGAAGCGTG GCTGGACGAT 
    CACTGCGACT TCACCCGCTC CTACTTTGTC AGGAAAGCCA CAAGAGAAAT GGTTAATGCA
    TGGTTTGCTG AGAGAGTTCA TACCATCCCT GTCTGCAAGG AGGGGACCAA GTCTCAGAGT
    GAATCCTGTG CTTGTCAGCA GCTTGCCTGC TCTGAGAACA CCAACTCTGG AACACCAGCA
    AGGAAAATCT CTGCTTCAGA ATTTGACAGA CCCTTAAGGC CGATTTTTGT CAAGGACTCA
    GTAGGAGCAG TAAGCTTCCT CTCTGGCTCT GAAAAGAAGG AACAGATGCC TCTGCAATCT
    CCAAGGACTG GGACCAGTGC AGGGGATCGG TGCTCAAGGC TTTTAGAACT TGTGAAAGAC
    ATTTCTAGTC ATTTGGATGT CACAGCACTT TGCCATAAAA TTTTCCTGCA TATCCATGAG
    CTTATAGCTG CTGATCGCTA TTCCCTCTTC TTGGTCTGTG AGGATAGCTC TAACGAGAAG
    TTTCTTGTAA GCAGGCTGTT TGATGTGGCT GAAGGTTCCA CCCTAGAAGA AGCTTCAAAT
    AACTGCATTC GCCTGGAGTG GAACAAGGGC ATCGTGGGCC ACGTAGCTGC TATAGGCCAG
    CCTCTGAACA TCAAGAATGC GTATGAGGAT CCAAGATTCA ATGCAGAAGT TGACCAGATC
    ACAGGGTATA AGACTCAGAG CATTCTCTGT ATGCCAATAA AGAATCATAG GGAAGAGGTT
    GTTGGTGTGG CTCAAGCAAT TAATAAGAAA TCTGGAACTG GTGGGACTTT CACGGAACAA
    GATGAAAAGG ATTTTGCTGC ATATTTGGCA TTTTGTGGAA TTGTTCTTCA CAACGCTCAA
    TTGTATGAAA CATCTTTGCT TGAAAACAGG CGTAATCAGG TGCTTCTTGA TCTTGCCAGC
    CTTATTTTTG AAGAACAACA GTCTTTGGAA GTAATTCTGA AGAAGATAGC TGCCACTATA
    ATATCCTTCA TGCAAGTGCA GAGGTGTACC ATCTTCATAG TGGACGAAGA TTGTACTGAT
    TCATTTTCTA GTGTTTTTCA TATGGAAGCT GAGGAATTAG AAGATTCAGC AGAAAACCAG
    AAGAGGGACT GTGATACAAA CAAAATCAAT TATATGTATG CTCAGTATGT CAAGAATACA
    ATGGAGCCTC TCAACATCCC AGATGTCTGC AAAGACAGAA GATTTCCCTG GACAACTGAC
    AGTGCAGAAA GTGTAAGTCA ACATATAAAG AGTTTGCTGT GTACACCAAT AAAAAATGGG
    AAAAAGAATA AGGTGATAGG GGTTTGCCAG CTTGTGAATA AGATGGAAGA AAACTCAGGC
    AAGATCAAGG CTTTCAACAG AAATGATGAG CAGTTTCTGG AAGCATTTGT CATCTTTTGT
    GGCTTGGGGA TCCAGAATAC ACAGATGTAT GAAGCTGTAG AAAGAGCAAT GGCCAAACAG
    ATGGTGACAT TAGAGGTTCT CTCATACCAT GCTTCTGCTG CTGAGGAGGA ATCAAGGGAG
    CTGCAGGTAA CAGCGGCTGC TGTAGTACCA TCTGCACAAT CTCTCAACCT AACTGACTTC
    AACTTCAGTG ATTTTGAGCT ATCAGATTTT GAAACAACAC TGTGTACAAT TCGAATGTTC
    ACAGACCTCA ACCTGGTGCA GAATTTCCAA ATGAAACATG AGGTTCTCTG CAGATGGATT
    TTAAGCGTGA AGAAAAATTA TCGGAAAAAT GTTGCTTATC ACAATTGGAG ACATGCCTTT
    AATACAGCAC AGTGCATGTT TGCTGCTTTA AAATCTGGTA AAATTCAGAG CAAACTGACT
    GACTTGGAAA CGCTGGCTTT GCTGATAGCA ACTCTAAGCC ATGATTTGGA TCACAGGGGA
    GTGAACAACT CTTACATACA AAGAAGTGAA CATCCACTGG CTCAACTGTA TTGCCACTCC
    ATCATGGAGC ATCACCATTT TGATCAATGC CTGATGATCC TGAATAGTCC AGGAAACCAG
    ATTCTCAGCA GCCTTTCAAT TGAAGAGTAC AAGGCAACAC TGAAAATGAT AAAACAAGCC
    ATCCTTGCAA CAGACCTAGC ACTATACATA AAGAGGAGAG GAGAATTTTT TGAACTTCTA
    AGGAAAAAGC AGTTTAATTG GGAAGATCCT ATGCAGAAGG AGCTATTTTT AGCAATGCTG
    ATGACTGCTT GTGATTTATC AGCTATAACC AAACCGTGGC CAGTTCAGCA ACGGATTGCT
    GAGCTTGTAG CTACTGAGTT CTTTGATCAG GGAGATAAAG AGCGGAAGGA ACTCAACATA
    GAACCAACAG ATCTAATGAA CAGAGAGAAG AAAAACAAAA TTCCAAGCAT GCAGGTTGGA
    TTCATTGATG CTGTTTGCTT GCAGCTGTAT GAGGCCCTAA CACATGTATC AGAGGCCTGC
    TACCCTTTAC TGGATGGCTG TAGAAAAAAC AGGCAGAAAT GGCAGTCCTT AGCTGAACAA
    CAAGAGAAGA ATCTGATTAA TGGAGAAAGC AATCAAGCCA AACGGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_420631.3
    CDS1945..4515
    Translation

    Target ORF information:

    RefSeq Version XM_420631.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus phosphodiesterase 5A (PDE5A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_420631.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGCA GCTGCCACCC CTCGCCTGAG CAGGAAGCGG TGGAAGCGTG GCTGGACGAT 
    CACTGCGACT TCACCCGCTC CTACTTTGTC AGGAAAGCCA CAAGAGAAAT GGTTAATGCA
    TGGTTTGCTG AGAGAGTTCA TACCATCCCT GTCTGCAAGG AGGGGACCAA GTCTCAGAGT
    GAATCCTGTG CTTGTCAGCA GCTTGCCTGC TCTGAGAACA CCAACTCTGG AACACCAGCA
    AGGAAAATCT CTGCTTCAGA ATTTGACAGA CCCTTAAGGC CGATTTTTGT CAAGGACTCA
    GTAGGAGCAG TAAGCTTCCT CTCTGGCTCT GAAAAGAAGG AACAGATGCC TCTGCAATCT
    CCAAGGACTG GGACCAGTGC AGGGGATCGG TGCTCAAGGC TTTTAGAACT TGTGAAAGAC
    ATTTCTAGTC ATTTGGATGT CACAGCACTT TGCCATAAAA TTTTCCTGCA TATCCATGAG
    CTTATAGCTG CTGATCGCTA TTCCCTCTTC TTGGTCTGTG AGGATAGCTC TAACGAGAAG
    TTTCTTGTAA GCAGGCTGTT TGATGTGGCT GAAGGTTCCA CCCTAGAAGA AGCTTCAAAT
    AACTGCATTC GCCTGGAGTG GAACAAGGGC ATCGTGGGCC ACGTAGCTGC TATAGGCCAG
    CCTCTGAACA TCAAGAATGC GTATGAGGAT CCAAGATTCA ATGCAGAAGT TGACCAGATC
    ACAGGGTATA AGACTCAGAG CATTCTCTGT ATGCCAATAA AGAATCATAG GGAAGAGGTT
    GTTGGTGTGG CTCAAGCAAT TAATAAGAAA TCTGGAACTG GTGGGACTTT CACGGAACAA
    GATGAAAAGG ATTTTGCTGC ATATTTGGCA TTTTGTGGAA TTGTTCTTCA CAACGCTCAA
    TTGTATGAAA CATCTTTGCT TGAAAACAGG CGTAATCAGG TGCTTCTTGA TCTTGCCAGC
    CTTATTTTTG AAGAACAACA GTCTTTGGAA GTAATTCTGA AGAAGATAGC TGCCACTATA
    ATATCCTTCA TGCAAGTGCA GAGGTGTACC ATCTTCATAG TGGACGAAGA TTGTACTGAT
    TCATTTTCTA GTGTTTTTCA TATGGAAGCT GAGGAATTAG AAGATTCAGC AGAAAACCAG
    AAGAGGGACT GTGATACAAA CAAAATCAAT TATATGTATG CTCAGTATGT CAAGAATACA
    ATGGAGCCTC TCAACATCCC AGATGTCTGC AAAGACAGAA GATTTCCCTG GACAACTGAC
    AGTGCAGAAA GTGTAAGTCA ACATATAAAG AGTTTGCTGT GTACACCAAT AAAAAATGGG
    AAAAAGAATA AGGTGATAGG GGTTTGCCAG CTTGTGAATA AGATGGAAGA AAACTCAGGC
    AAGATCAAGG CTTTCAACAG AAATGATGAG CAGTTTCTGG AAGCATTTGT CATCTTTTGT
    GGCTTGGGGA TCCAGAATAC ACAGATGTAT GAAGCTGTAG AAAGAGCAAT GGCCAAACAG
    ATGGTGACAT TAGAGGTTCT CTCATACCAT GCTTCTGCTG CTGAGGAGGA ATCAAGGGAG
    CTGCAGGTAA CAGCGGCTGC TGTAGTACCA TCTGCACAAT CTCTCAACCT AACTGACTTC
    AACTTCAGTG ATTTTGAGCT ATCAGATTTT GAAACAACAC TGTGTACAAT TCGAATGTTC
    ACAGACCTCA ACCTGGTGCA GAATTTCCAA ATGAAACATG AGGTTCTCTG CAGATGGATT
    TTAAGCGTGA AGAAAAATTA TCGGAAAAAT GTTGCTTATC ACAATTGGAG ACATGCCTTT
    AATACAGCAC AGTGCATGTT TGCTGCTTTA AAATCTGGTA AAATTCAGAG CAAACTGACT
    GACTTGGAAA CGCTGGCTTT GCTGATAGCA ACTCTAAGCC ATGATTTGGA TCACAGGGGA
    GTGAACAACT CTTACATACA AAGAAGTGAA CATCCACTGG CTCAACTGTA TTGCCACTCC
    ATCATGGAGC ATCACCATTT TGATCAATGC CTGATGATCC TGAATAGTCC AGGAAACCAG
    ATTCTCAGCA GCCTTTCAAT TGAAGAGTAC AAGGCAACAC TGAAAATGAT AAAACAAGCC
    ATCCTTGCAA CAGACCTAGC ACTATACATA AAGAGGAGAG GAGAATTTTT TGAACTTCTA
    AGGAAAAAGC AGTTTAATTG GGAAGATCCT ATGCAGAAGG AGCTATTTTT AGCAATGCTG
    ATGACTGCTT GTGATTTATC AGCTATAACC AAACCGTGGC CAGTTCAGCA ACGGATTGCT
    GAGCTTGTAG CTACTGAGTT CTTTGATCAG GGAGATAAAG AGCGGAAGGA ACTCAACATA
    GAACCAACAG ATCTAATGAA CAGAGAGAAG AAAAACAAAA TTCCAAGCAT GCAGGTTGGA
    TTCATTGATG CTGTTTGCTT GCAGCTGTAT GAGGCCCTAA CACATGTATC AGAGGCCTGC
    TACCCTTTAC TGGATGGCTG TAGAAAAAAC AGGCAGAAAT GGCAGTCCTT AGCTGAACAA
    CAAGAGAAGA ATCTGATTAA TGGAGAAAGC AATCAAGCCA AACGGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa08194
    Clone ID Related Accession (Same CDS sequence) XM_004941236.3 , XM_004941236.2
    Accession Version XM_004941236.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004941236.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGCA GCTGCCACCC CTCGCCTGAG CAGGAAGCGG TGGAAGCGTG GCTGGACGAT 
    CACTGCGACT TCACCCGCTC CTACTTTGTC AGGAAAGCCA CAAGAGAAAT GGTTAATGCA
    TGGTTTGCTG AGAGAGTTCA TACCATCCCT GTCTGCAAGG AGGGGACCAA GTCTCAGAGT
    GAATCCTGTG CTTGTCAGCA GCTTGCCTGC TCTGAGAACA CCAACTCTGG AACACCAGCA
    AGGAAAATCT CTGCTTCAGA ATTTGACAGA CCCTTAAGGC CGATTTTTGT CAAGGACTCA
    GTAGGAGCAG TAAGCTTCCT CTCTGGCTCT GAAAAGAAGG AACAGATGCC TCTGCAATCT
    CCAAGGACTG GGACCAGTGC AGGGGATCGG TGCTCAAGGC TTTTAGAACT TGTGAAAGAC
    ATTTCTAGTC ATTTGGATGT CACAGCACTT TGCCATAAAA TTTTCCTGCA TATCCATGAG
    CTTATAGCTG CTGATCGCTA TTCCCTCTTC TTGGTCTGTG AGGATAGCTC TAACGAGAAG
    TTTCTTGTAA GCAGGCTGTT TGATGTGGCT GAAGGTTCCA CCCTAGAAGA AGCTTCAAAT
    AACTGCATTC GCCTGGAGTG GAACAAGGGC ATCGTGGGCC ACGTAGCTGC TATAGGCCAG
    CCTCTGAACA TCAAGAATGC GTATGAGGAT CCAAGATTCA ATGCAGAAGT TGACCAGATC
    ACAGGGTATA AGACTCAGAG CATTCTCTGT ATGCCAATAA AGAATCATAG GGAAGAGGTT
    GTTGGTGTGG CTCAAGCAAT TAATAAGAAA TCTGGAACTG GTGGGACTTT CACGGAACAA
    GATGAAAAGG ATTTTGCTGC ATATTTGGCA TTTTGTGGAA TTGTTCTTCA CAACGCTCAA
    TTGTATGAAA CATCTTTGCT TGAAAACAGG CGTAATCAGG TGCTTCTTGA TCTTGCCAGC
    CTTATTTTTG AAGAACAACA GTCTTTGGAA GTAATTCTGA AGAAGATAGC TGCCACTATA
    ATATCCTTCA TGCAAGTGCA GAGGTGTACC ATCTTCATAG TGGACGAAGA TTGTACTGAT
    TCATTTTCTA GTGTTTTTCA TATGGAAGCT GAGGAATTAG AAGATTCAGC AGAAAACCAG
    AAGAGGGACT GTGATACAAA CAAAATCAAT TATATGTATG CTCAGTATGT CAAGAATACA
    ATGGAGCCTC TCAACATCCC AGATGTCTGC AAAGACAGAA GATTTCCCTG GACAACTGAC
    AGTGCAGAAA GTGTAAGTCA ACATATAAAG AGTTTGCTGT GTACACCAAT AAAAAATGGG
    AAAAAGAATA AGGTGATAGG GGTTTGCCAG CTTGTGAATA AGATGGAAGA AAACTCAGGC
    AAGATCAAGG CTTTCAACAG AAATGATGAG CAGTTTCTGG AAGCATTTGT CATCTTTTGT
    GGCTTGGGGA TCCAGAATAC ACAGATGTAT GAAGCTGTAG AAAGAGCAAT GGCCAAACAG
    ATGGTGACAT TAGAGGTTCT CTCATACCAT GCTTCTGCTG CTGAGGAGGA ATCAAGGGAG
    CTGCAGGCTG CTGTAGTACC ATCTGCACAA TCTCTCAACC TAACTGACTT CAACTTCAGT
    GATTTTGAGC TATCAGATTT TGAAACAACA CTGTGTACAA TTCGAATGTT CACAGACCTC
    AACCTGGTGC AGAATTTCCA AATGAAACAT GAGGTTCTCT GCAGATGGAT TTTAAGCGTG
    AAGAAAAATT ATCGGAAAAA TGTTGCTTAT CACAATTGGA GACATGCCTT TAATACAGCA
    CAGTGCATGT TTGCTGCTTT AAAATCTGGT AAAATTCAGA GCAAACTGAC TGACTTGGAA
    ACGCTGGCTT TGCTGATAGC AACTCTAAGC CATGATTTGG ATCACAGGGG AGTGAACAAC
    TCTTACATAC AAAGAAGTGA ACATCCACTG GCTCAACTGT ATTGCCACTC CATCATGGAG
    CATCACCATT TTGATCAATG CCTGATGATC CTGAATAGTC CAGGAAACCA GATTCTCAGC
    AGCCTTTCAA TTGAAGAGTA CAAGGCAACA CTGAAAATGA TAAAACAAGC CATCCTTGCA
    ACAGACCTAG CACTATACAT AAAGAGGAGA GGAGAATTTT TTGAACTTCT AAGGAAAAAG
    CAGTTTAATT GGGAAGATCC TATGCAGAAG GAGCTATTTT TAGCAATGCT GATGACTGCT
    TGTGATTTAT CAGCTATAAC CAAACCGTGG CCAGTTCAGC AACGGATTGC TGAGCTTGTA
    GCTACTGAGT TCTTTGATCA GGGAGATAAA GAGCGGAAGG AACTCAACAT AGAACCAACA
    GATCTAATGA ACAGAGAGAA GAAAAACAAA ATTCCAAGCA TGCAGGTTGG ATTCATTGAT
    GCTGTTTGCT TGCAGCTGTA TGAGGCCCTA ACACATGTAT CAGAGGCCTG CTACCCTTTA
    CTGGATGGCT GTAGAAAAAA CAGGCAGAAA TGGCAGTCCT TAGCTGAACA ACAAGAGAAG
    AATCTGATTA ATGGAGAAAG CAATCAAGCC AAACGGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004941293.1
    CDS1945..4506
    Translation

    Target ORF information:

    RefSeq Version XM_004941236.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus phosphodiesterase 5A (PDE5A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004941236.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGCA GCTGCCACCC CTCGCCTGAG CAGGAAGCGG TGGAAGCGTG GCTGGACGAT 
    CACTGCGACT TCACCCGCTC CTACTTTGTC AGGAAAGCCA CAAGAGAAAT GGTTAATGCA
    TGGTTTGCTG AGAGAGTTCA TACCATCCCT GTCTGCAAGG AGGGGACCAA GTCTCAGAGT
    GAATCCTGTG CTTGTCAGCA GCTTGCCTGC TCTGAGAACA CCAACTCTGG AACACCAGCA
    AGGAAAATCT CTGCTTCAGA ATTTGACAGA CCCTTAAGGC CGATTTTTGT CAAGGACTCA
    GTAGGAGCAG TAAGCTTCCT CTCTGGCTCT GAAAAGAAGG AACAGATGCC TCTGCAATCT
    CCAAGGACTG GGACCAGTGC AGGGGATCGG TGCTCAAGGC TTTTAGAACT TGTGAAAGAC
    ATTTCTAGTC ATTTGGATGT CACAGCACTT TGCCATAAAA TTTTCCTGCA TATCCATGAG
    CTTATAGCTG CTGATCGCTA TTCCCTCTTC TTGGTCTGTG AGGATAGCTC TAACGAGAAG
    TTTCTTGTAA GCAGGCTGTT TGATGTGGCT GAAGGTTCCA CCCTAGAAGA AGCTTCAAAT
    AACTGCATTC GCCTGGAGTG GAACAAGGGC ATCGTGGGCC ACGTAGCTGC TATAGGCCAG
    CCTCTGAACA TCAAGAATGC GTATGAGGAT CCAAGATTCA ATGCAGAAGT TGACCAGATC
    ACAGGGTATA AGACTCAGAG CATTCTCTGT ATGCCAATAA AGAATCATAG GGAAGAGGTT
    GTTGGTGTGG CTCAAGCAAT TAATAAGAAA TCTGGAACTG GTGGGACTTT CACGGAACAA
    GATGAAAAGG ATTTTGCTGC ATATTTGGCA TTTTGTGGAA TTGTTCTTCA CAACGCTCAA
    TTGTATGAAA CATCTTTGCT TGAAAACAGG CGTAATCAGG TGCTTCTTGA TCTTGCCAGC
    CTTATTTTTG AAGAACAACA GTCTTTGGAA GTAATTCTGA AGAAGATAGC TGCCACTATA
    ATATCCTTCA TGCAAGTGCA GAGGTGTACC ATCTTCATAG TGGACGAAGA TTGTACTGAT
    TCATTTTCTA GTGTTTTTCA TATGGAAGCT GAGGAATTAG AAGATTCAGC AGAAAACCAG
    AAGAGGGACT GTGATACAAA CAAAATCAAT TATATGTATG CTCAGTATGT CAAGAATACA
    ATGGAGCCTC TCAACATCCC AGATGTCTGC AAAGACAGAA GATTTCCCTG GACAACTGAC
    AGTGCAGAAA GTGTAAGTCA ACATATAAAG AGTTTGCTGT GTACACCAAT AAAAAATGGG
    AAAAAGAATA AGGTGATAGG GGTTTGCCAG CTTGTGAATA AGATGGAAGA AAACTCAGGC
    AAGATCAAGG CTTTCAACAG AAATGATGAG CAGTTTCTGG AAGCATTTGT CATCTTTTGT
    GGCTTGGGGA TCCAGAATAC ACAGATGTAT GAAGCTGTAG AAAGAGCAAT GGCCAAACAG
    ATGGTGACAT TAGAGGTTCT CTCATACCAT GCTTCTGCTG CTGAGGAGGA ATCAAGGGAG
    CTGCAGGCTG CTGTAGTACC ATCTGCACAA TCTCTCAACC TAACTGACTT CAACTTCAGT
    GATTTTGAGC TATCAGATTT TGAAACAACA CTGTGTACAA TTCGAATGTT CACAGACCTC
    AACCTGGTGC AGAATTTCCA AATGAAACAT GAGGTTCTCT GCAGATGGAT TTTAAGCGTG
    AAGAAAAATT ATCGGAAAAA TGTTGCTTAT CACAATTGGA GACATGCCTT TAATACAGCA
    CAGTGCATGT TTGCTGCTTT AAAATCTGGT AAAATTCAGA GCAAACTGAC TGACTTGGAA
    ACGCTGGCTT TGCTGATAGC AACTCTAAGC CATGATTTGG ATCACAGGGG AGTGAACAAC
    TCTTACATAC AAAGAAGTGA ACATCCACTG GCTCAACTGT ATTGCCACTC CATCATGGAG
    CATCACCATT TTGATCAATG CCTGATGATC CTGAATAGTC CAGGAAACCA GATTCTCAGC
    AGCCTTTCAA TTGAAGAGTA CAAGGCAACA CTGAAAATGA TAAAACAAGC CATCCTTGCA
    ACAGACCTAG CACTATACAT AAAGAGGAGA GGAGAATTTT TTGAACTTCT AAGGAAAAAG
    CAGTTTAATT GGGAAGATCC TATGCAGAAG GAGCTATTTT TAGCAATGCT GATGACTGCT
    TGTGATTTAT CAGCTATAAC CAAACCGTGG CCAGTTCAGC AACGGATTGC TGAGCTTGTA
    GCTACTGAGT TCTTTGATCA GGGAGATAAA GAGCGGAAGG AACTCAACAT AGAACCAACA
    GATCTAATGA ACAGAGAGAA GAAAAACAAA ATTCCAAGCA TGCAGGTTGG ATTCATTGAT
    GCTGTTTGCT TGCAGCTGTA TGAGGCCCTA ACACATGTAT CAGAGGCCTG CTACCCTTTA
    CTGGATGGCT GTAGAAAAAA CAGGCAGAAA TGGCAGTCCT TAGCTGAACA ACAAGAGAAG
    AATCTGATTA ATGGAGAAAG CAATCAAGCC AAACGGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa08194
    Clone ID Related Accession (Same CDS sequence) XM_004941236.3 , XM_004941236.2
    Accession Version XM_004941236.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004941236.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGCA GCTGCCACCC CTCGCCTGAG CAGGAAGCGG TGGAAGCGTG GCTGGACGAT 
    CACTGCGACT TCACCCGCTC CTACTTTGTC AGGAAAGCCA CAAGAGAAAT GGTTAATGCA
    TGGTTTGCTG AGAGAGTTCA TACCATCCCT GTCTGCAAGG AGGGGACCAA GTCTCAGAGT
    GAATCCTGTG CTTGTCAGCA GCTTGCCTGC TCTGAGAACA CCAACTCTGG AACACCAGCA
    AGGAAAATCT CTGCTTCAGA ATTTGACAGA CCCTTAAGGC CGATTTTTGT CAAGGACTCA
    GTAGGAGCAG TAAGCTTCCT CTCTGGCTCT GAAAAGAAGG AACAGATGCC TCTGCAATCT
    CCAAGGACTG GGACCAGTGC AGGGGATCGG TGCTCAAGGC TTTTAGAACT TGTGAAAGAC
    ATTTCTAGTC ATTTGGATGT CACAGCACTT TGCCATAAAA TTTTCCTGCA TATCCATGAG
    CTTATAGCTG CTGATCGCTA TTCCCTCTTC TTGGTCTGTG AGGATAGCTC TAACGAGAAG
    TTTCTTGTAA GCAGGCTGTT TGATGTGGCT GAAGGTTCCA CCCTAGAAGA AGCTTCAAAT
    AACTGCATTC GCCTGGAGTG GAACAAGGGC ATCGTGGGCC ACGTAGCTGC TATAGGCCAG
    CCTCTGAACA TCAAGAATGC GTATGAGGAT CCAAGATTCA ATGCAGAAGT TGACCAGATC
    ACAGGGTATA AGACTCAGAG CATTCTCTGT ATGCCAATAA AGAATCATAG GGAAGAGGTT
    GTTGGTGTGG CTCAAGCAAT TAATAAGAAA TCTGGAACTG GTGGGACTTT CACGGAACAA
    GATGAAAAGG ATTTTGCTGC ATATTTGGCA TTTTGTGGAA TTGTTCTTCA CAACGCTCAA
    TTGTATGAAA CATCTTTGCT TGAAAACAGG CGTAATCAGG TGCTTCTTGA TCTTGCCAGC
    CTTATTTTTG AAGAACAACA GTCTTTGGAA GTAATTCTGA AGAAGATAGC TGCCACTATA
    ATATCCTTCA TGCAAGTGCA GAGGTGTACC ATCTTCATAG TGGACGAAGA TTGTACTGAT
    TCATTTTCTA GTGTTTTTCA TATGGAAGCT GAGGAATTAG AAGATTCAGC AGAAAACCAG
    AAGAGGGACT GTGATACAAA CAAAATCAAT TATATGTATG CTCAGTATGT CAAGAATACA
    ATGGAGCCTC TCAACATCCC AGATGTCTGC AAAGACAGAA GATTTCCCTG GACAACTGAC
    AGTGCAGAAA GTGTAAGTCA ACATATAAAG AGTTTGCTGT GTACACCAAT AAAAAATGGG
    AAAAAGAATA AGGTGATAGG GGTTTGCCAG CTTGTGAATA AGATGGAAGA AAACTCAGGC
    AAGATCAAGG CTTTCAACAG AAATGATGAG CAGTTTCTGG AAGCATTTGT CATCTTTTGT
    GGCTTGGGGA TCCAGAATAC ACAGATGTAT GAAGCTGTAG AAAGAGCAAT GGCCAAACAG
    ATGGTGACAT TAGAGGTTCT CTCATACCAT GCTTCTGCTG CTGAGGAGGA ATCAAGGGAG
    CTGCAGGCTG CTGTAGTACC ATCTGCACAA TCTCTCAACC TAACTGACTT CAACTTCAGT
    GATTTTGAGC TATCAGATTT TGAAACAACA CTGTGTACAA TTCGAATGTT CACAGACCTC
    AACCTGGTGC AGAATTTCCA AATGAAACAT GAGGTTCTCT GCAGATGGAT TTTAAGCGTG
    AAGAAAAATT ATCGGAAAAA TGTTGCTTAT CACAATTGGA GACATGCCTT TAATACAGCA
    CAGTGCATGT TTGCTGCTTT AAAATCTGGT AAAATTCAGA GCAAACTGAC TGACTTGGAA
    ACGCTGGCTT TGCTGATAGC AACTCTAAGC CATGATTTGG ATCACAGGGG AGTGAACAAC
    TCTTACATAC AAAGAAGTGA ACATCCACTG GCTCAACTGT ATTGCCACTC CATCATGGAG
    CATCACCATT TTGATCAATG CCTGATGATC CTGAATAGTC CAGGAAACCA GATTCTCAGC
    AGCCTTTCAA TTGAAGAGTA CAAGGCAACA CTGAAAATGA TAAAACAAGC CATCCTTGCA
    ACAGACCTAG CACTATACAT AAAGAGGAGA GGAGAATTTT TTGAACTTCT AAGGAAAAAG
    CAGTTTAATT GGGAAGATCC TATGCAGAAG GAGCTATTTT TAGCAATGCT GATGACTGCT
    TGTGATTTAT CAGCTATAAC CAAACCGTGG CCAGTTCAGC AACGGATTGC TGAGCTTGTA
    GCTACTGAGT TCTTTGATCA GGGAGATAAA GAGCGGAAGG AACTCAACAT AGAACCAACA
    GATCTAATGA ACAGAGAGAA GAAAAACAAA ATTCCAAGCA TGCAGGTTGG ATTCATTGAT
    GCTGTTTGCT TGCAGCTGTA TGAGGCCCTA ACACATGTAT CAGAGGCCTG CTACCCTTTA
    CTGGATGGCT GTAGAAAAAA CAGGCAGAAA TGGCAGTCCT TAGCTGAACA ACAAGAGAAG
    AATCTGATTA ATGGAGAAAG CAATCAAGCC AAACGGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004941293.1
    CDS1767..4328
    Translation

    Target ORF information:

    RefSeq Version XM_004941236.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004941236.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGCA GCTGCCACCC CTCGCCTGAG CAGGAAGCGG TGGAAGCGTG GCTGGACGAT 
    CACTGCGACT TCACCCGCTC CTACTTTGTC AGGAAAGCCA CAAGAGAAAT GGTTAATGCA
    TGGTTTGCTG AGAGAGTTCA TACCATCCCT GTCTGCAAGG AGGGGACCAA GTCTCAGAGT
    GAATCCTGTG CTTGTCAGCA GCTTGCCTGC TCTGAGAACA CCAACTCTGG AACACCAGCA
    AGGAAAATCT CTGCTTCAGA ATTTGACAGA CCCTTAAGGC CGATTTTTGT CAAGGACTCA
    GTAGGAGCAG TAAGCTTCCT CTCTGGCTCT GAAAAGAAGG AACAGATGCC TCTGCAATCT
    CCAAGGACTG GGACCAGTGC AGGGGATCGG TGCTCAAGGC TTTTAGAACT TGTGAAAGAC
    ATTTCTAGTC ATTTGGATGT CACAGCACTT TGCCATAAAA TTTTCCTGCA TATCCATGAG
    CTTATAGCTG CTGATCGCTA TTCCCTCTTC TTGGTCTGTG AGGATAGCTC TAACGAGAAG
    TTTCTTGTAA GCAGGCTGTT TGATGTGGCT GAAGGTTCCA CCCTAGAAGA AGCTTCAAAT
    AACTGCATTC GCCTGGAGTG GAACAAGGGC ATCGTGGGCC ACGTAGCTGC TATAGGCCAG
    CCTCTGAACA TCAAGAATGC GTATGAGGAT CCAAGATTCA ATGCAGAAGT TGACCAGATC
    ACAGGGTATA AGACTCAGAG CATTCTCTGT ATGCCAATAA AGAATCATAG GGAAGAGGTT
    GTTGGTGTGG CTCAAGCAAT TAATAAGAAA TCTGGAACTG GTGGGACTTT CACGGAACAA
    GATGAAAAGG ATTTTGCTGC ATATTTGGCA TTTTGTGGAA TTGTTCTTCA CAACGCTCAA
    TTGTATGAAA CATCTTTGCT TGAAAACAGG CGTAATCAGG TGCTTCTTGA TCTTGCCAGC
    CTTATTTTTG AAGAACAACA GTCTTTGGAA GTAATTCTGA AGAAGATAGC TGCCACTATA
    ATATCCTTCA TGCAAGTGCA GAGGTGTACC ATCTTCATAG TGGACGAAGA TTGTACTGAT
    TCATTTTCTA GTGTTTTTCA TATGGAAGCT GAGGAATTAG AAGATTCAGC AGAAAACCAG
    AAGAGGGACT GTGATACAAA CAAAATCAAT TATATGTATG CTCAGTATGT CAAGAATACA
    ATGGAGCCTC TCAACATCCC AGATGTCTGC AAAGACAGAA GATTTCCCTG GACAACTGAC
    AGTGCAGAAA GTGTAAGTCA ACATATAAAG AGTTTGCTGT GTACACCAAT AAAAAATGGG
    AAAAAGAATA AGGTGATAGG GGTTTGCCAG CTTGTGAATA AGATGGAAGA AAACTCAGGC
    AAGATCAAGG CTTTCAACAG AAATGATGAG CAGTTTCTGG AAGCATTTGT CATCTTTTGT
    GGCTTGGGGA TCCAGAATAC ACAGATGTAT GAAGCTGTAG AAAGAGCAAT GGCCAAACAG
    ATGGTGACAT TAGAGGTTCT CTCATACCAT GCTTCTGCTG CTGAGGAGGA ATCAAGGGAG
    CTGCAGGCTG CTGTAGTACC ATCTGCACAA TCTCTCAACC TAACTGACTT CAACTTCAGT
    GATTTTGAGC TATCAGATTT TGAAACAACA CTGTGTACAA TTCGAATGTT CACAGACCTC
    AACCTGGTGC AGAATTTCCA AATGAAACAT GAGGTTCTCT GCAGATGGAT TTTAAGCGTG
    AAGAAAAATT ATCGGAAAAA TGTTGCTTAT CACAATTGGA GACATGCCTT TAATACAGCA
    CAGTGCATGT TTGCTGCTTT AAAATCTGGT AAAATTCAGA GCAAACTGAC TGACTTGGAA
    ACGCTGGCTT TGCTGATAGC AACTCTAAGC CATGATTTGG ATCACAGGGG AGTGAACAAC
    TCTTACATAC AAAGAAGTGA ACATCCACTG GCTCAACTGT ATTGCCACTC CATCATGGAG
    CATCACCATT TTGATCAATG CCTGATGATC CTGAATAGTC CAGGAAACCA GATTCTCAGC
    AGCCTTTCAA TTGAAGAGTA CAAGGCAACA CTGAAAATGA TAAAACAAGC CATCCTTGCA
    ACAGACCTAG CACTATACAT AAAGAGGAGA GGAGAATTTT TTGAACTTCT AAGGAAAAAG
    CAGTTTAATT GGGAAGATCC TATGCAGAAG GAGCTATTTT TAGCAATGCT GATGACTGCT
    TGTGATTTAT CAGCTATAAC CAAACCGTGG CCAGTTCAGC AACGGATTGC TGAGCTTGTA
    GCTACTGAGT TCTTTGATCA GGGAGATAAA GAGCGGAAGG AACTCAACAT AGAACCAACA
    GATCTAATGA ACAGAGAGAA GAAAAACAAA ATTCCAAGCA TGCAGGTTGG ATTCATTGAT
    GCTGTTTGCT TGCAGCTGTA TGAGGCCCTA ACACATGTAT CAGAGGCCTG CTACCCTTTA
    CTGGATGGCT GTAGAAAAAA CAGGCAGAAA TGGCAGTCCT TAGCTGAACA ACAAGAGAAG
    AATCTGATTA ATGGAGAAAG CAATCAAGCC AAACGGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa08195
    Clone ID Related Accession (Same CDS sequence) XM_004941237.2 , XM_004941237.3
    Accession Version XM_004941237.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004941237.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTGCCTT TTGGAGATAA GACGAGAGAA ATGGTTAATG CATGGTTTGC TGAGAGAGTT 
    CATACCATCC CTGTCTGCAA GGAGGGGACC AAGTCTCAGA GTGAATCCTG TGCTTGTCAG
    CAGCTTGCCT GCTCTGAGAA CACCAACTCT GGAACACCAG CAAGGAAAAT CTCTGCTTCA
    GAATTTGACA GACCCTTAAG GCCGATTTTT GTCAAGGACT CAGTAGGAGC AGTAAGCTTC
    CTCTCTGGCT CTGAAAAGAA GGAACAGATG CCTCTGCAAT CTCCAAGGAC TGGGACCAGT
    GCAGGGGATC GGTGCTCAAG GCTTTTAGAA CTTGTGAAAG ACATTTCTAG TCATTTGGAT
    GTCACAGCAC TTTGCCATAA AATTTTCCTG CATATCCATG AGCTTATAGC TGCTGATCGC
    TATTCCCTCT TCTTGGTCTG TGAGGATAGC TCTAACGAGA AGTTTCTTGT AAGCAGGCTG
    TTTGATGTGG CTGAAGGTTC CACCCTAGAA GAAGCTTCAA ATAACTGCAT TCGCCTGGAG
    TGGAACAAGG GCATCGTGGG CCACGTAGCT GCTATAGGCC AGCCTCTGAA CATCAAGAAT
    GCGTATGAGG ATCCAAGATT CAATGCAGAA GTTGACCAGA TCACAGGGTA TAAGACTCAG
    AGCATTCTCT GTATGCCAAT AAAGAATCAT AGGGAAGAGG TTGTTGGTGT GGCTCAAGCA
    ATTAATAAGA AATCTGGAAC TGGTGGGACT TTCACGGAAC AAGATGAAAA GGATTTTGCT
    GCATATTTGG CATTTTGTGG AATTGTTCTT CACAACGCTC AATTGTATGA AACATCTTTG
    CTTGAAAACA GGCGTAATCA GGTGCTTCTT GATCTTGCCA GCCTTATTTT TGAAGAACAA
    CAGTCTTTGG AAGTAATTCT GAAGAAGATA GCTGCCACTA TAATATCCTT CATGCAAGTG
    CAGAGGTGTA CCATCTTCAT AGTGGACGAA GATTGTACTG ATTCATTTTC TAGTGTTTTT
    CATATGGAAG CTGAGGAATT AGAAGATTCA GCAGAAAACC AGAAGAGGGA CTGTGATACA
    AACAAAATCA ATTATATGTA TGCTCAGTAT GTCAAGAATA CAATGGAGCC TCTCAACATC
    CCAGATGTCT GCAAAGACAG AAGATTTCCC TGGACAACTG ACAGTGCAGA AAGTGTAAGT
    CAACATATAA AGAGTTTGCT GTGTACACCA ATAAAAAATG GGAAAAAGAA TAAGGTGATA
    GGGGTTTGCC AGCTTGTGAA TAAGATGGAA GAAAACTCAG GCAAGATCAA GGCTTTCAAC
    AGAAATGATG AGCAGTTTCT GGAAGCATTT GTCATCTTTT GTGGCTTGGG GATCCAGAAT
    ACACAGATGT ATGAAGCTGT AGAAAGAGCA ATGGCCAAAC AGATGGTGAC ATTAGAGGTT
    CTCTCATACC ATGCTTCTGC TGCTGAGGAG GAATCAAGGG AGCTGCAGGT AACAGCGGCT
    GCTGTAGTAC CATCTGCACA ATCTCTCAAC CTAACTGACT TCAACTTCAG TGATTTTGAG
    CTATCAGATT TTGAAACAAC ACTGTGTACA ATTCGAATGT TCACAGACCT CAACCTGGTG
    CAGAATTTCC AAATGAAACA TGAGGTTCTC TGCAGATGGA TTTTAAGCGT GAAGAAAAAT
    TATCGGAAAA ATGTTGCTTA TCACAATTGG AGACATGCCT TTAATACAGC ACAGTGCATG
    TTTGCTGCTT TAAAATCTGG TAAAATTCAG AGCAAACTGA CTGACTTGGA AACGCTGGCT
    TTGCTGATAG CAACTCTAAG CCATGATTTG GATCACAGGG GAGTGAACAA CTCTTACATA
    CAAAGAAGTG AACATCCACT GGCTCAACTG TATTGCCACT CCATCATGGA GCATCACCAT
    TTTGATCAAT GCCTGATGAT CCTGAATAGT CCAGGAAACC AGATTCTCAG CAGCCTTTCA
    ATTGAAGAGT ACAAGGCAAC ACTGAAAATG ATAAAACAAG CCATCCTTGC AACAGACCTA
    GCACTATACA TAAAGAGGAG AGGAGAATTT TTTGAACTTC TAAGGAAAAA GCAGTTTAAT
    TGGGAAGATC CTATGCAGAA GGAGCTATTT TTAGCAATGC TGATGACTGC TTGTGATTTA
    TCAGCTATAA CCAAACCGTG GCCAGTTCAG CAACGGATTG CTGAGCTTGT AGCTACTGAG
    TTCTTTGATC AGGGAGATAA AGAGCGGAAG GAACTCAACA TAGAACCAAC AGATCTAATG
    AACAGAGAGA AGAAAAACAA AATTCCAAGC ATGCAGGTTG GATTCATTGA TGCTGTTTGC
    TTGCAGCTGT ATGAGGCCCT AACACATGTA TCAGAGGCCT GCTACCCTTT ACTGGATGGC
    TGTAGAAAAA ACAGGCAGAA ATGGCAGTCC TTAGCTGAAC AACAAGAGAA GAATCTGATT
    AATGGAGAAA GCAATCAAGC CAAACGGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004941294.1
    CDS163..2655
    Translation

    Target ORF information:

    RefSeq Version XM_004941237.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004941237.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTGCCTT TTGGAGATAA GACGAGAGAA ATGGTTAATG CATGGTTTGC TGAGAGAGTT 
    CATACCATCC CTGTCTGCAA GGAGGGGACC AAGTCTCAGA GTGAATCCTG TGCTTGTCAG
    CAGCTTGCCT GCTCTGAGAA CACCAACTCT GGAACACCAG CAAGGAAAAT CTCTGCTTCA
    GAATTTGACA GACCCTTAAG GCCGATTTTT GTCAAGGACT CAGTAGGAGC AGTAAGCTTC
    CTCTCTGGCT CTGAAAAGAA GGAACAGATG CCTCTGCAAT CTCCAAGGAC TGGGACCAGT
    GCAGGGGATC GGTGCTCAAG GCTTTTAGAA CTTGTGAAAG ACATTTCTAG TCATTTGGAT
    GTCACAGCAC TTTGCCATAA AATTTTCCTG CATATCCATG AGCTTATAGC TGCTGATCGC
    TATTCCCTCT TCTTGGTCTG TGAGGATAGC TCTAACGAGA AGTTTCTTGT AAGCAGGCTG
    TTTGATGTGG CTGAAGGTTC CACCCTAGAA GAAGCTTCAA ATAACTGCAT TCGCCTGGAG
    TGGAACAAGG GCATCGTGGG CCACGTAGCT GCTATAGGCC AGCCTCTGAA CATCAAGAAT
    GCGTATGAGG ATCCAAGATT CAATGCAGAA GTTGACCAGA TCACAGGGTA TAAGACTCAG
    AGCATTCTCT GTATGCCAAT AAAGAATCAT AGGGAAGAGG TTGTTGGTGT GGCTCAAGCA
    ATTAATAAGA AATCTGGAAC TGGTGGGACT TTCACGGAAC AAGATGAAAA GGATTTTGCT
    GCATATTTGG CATTTTGTGG AATTGTTCTT CACAACGCTC AATTGTATGA AACATCTTTG
    CTTGAAAACA GGCGTAATCA GGTGCTTCTT GATCTTGCCA GCCTTATTTT TGAAGAACAA
    CAGTCTTTGG AAGTAATTCT GAAGAAGATA GCTGCCACTA TAATATCCTT CATGCAAGTG
    CAGAGGTGTA CCATCTTCAT AGTGGACGAA GATTGTACTG ATTCATTTTC TAGTGTTTTT
    CATATGGAAG CTGAGGAATT AGAAGATTCA GCAGAAAACC AGAAGAGGGA CTGTGATACA
    AACAAAATCA ATTATATGTA TGCTCAGTAT GTCAAGAATA CAATGGAGCC TCTCAACATC
    CCAGATGTCT GCAAAGACAG AAGATTTCCC TGGACAACTG ACAGTGCAGA AAGTGTAAGT
    CAACATATAA AGAGTTTGCT GTGTACACCA ATAAAAAATG GGAAAAAGAA TAAGGTGATA
    GGGGTTTGCC AGCTTGTGAA TAAGATGGAA GAAAACTCAG GCAAGATCAA GGCTTTCAAC
    AGAAATGATG AGCAGTTTCT GGAAGCATTT GTCATCTTTT GTGGCTTGGG GATCCAGAAT
    ACACAGATGT ATGAAGCTGT AGAAAGAGCA ATGGCCAAAC AGATGGTGAC ATTAGAGGTT
    CTCTCATACC ATGCTTCTGC TGCTGAGGAG GAATCAAGGG AGCTGCAGGT AACAGCGGCT
    GCTGTAGTAC CATCTGCACA ATCTCTCAAC CTAACTGACT TCAACTTCAG TGATTTTGAG
    CTATCAGATT TTGAAACAAC ACTGTGTACA ATTCGAATGT TCACAGACCT CAACCTGGTG
    CAGAATTTCC AAATGAAACA TGAGGTTCTC TGCAGATGGA TTTTAAGCGT GAAGAAAAAT
    TATCGGAAAA ATGTTGCTTA TCACAATTGG AGACATGCCT TTAATACAGC ACAGTGCATG
    TTTGCTGCTT TAAAATCTGG TAAAATTCAG AGCAAACTGA CTGACTTGGA AACGCTGGCT
    TTGCTGATAG CAACTCTAAG CCATGATTTG GATCACAGGG GAGTGAACAA CTCTTACATA
    CAAAGAAGTG AACATCCACT GGCTCAACTG TATTGCCACT CCATCATGGA GCATCACCAT
    TTTGATCAAT GCCTGATGAT CCTGAATAGT CCAGGAAACC AGATTCTCAG CAGCCTTTCA
    ATTGAAGAGT ACAAGGCAAC ACTGAAAATG ATAAAACAAG CCATCCTTGC AACAGACCTA
    GCACTATACA TAAAGAGGAG AGGAGAATTT TTTGAACTTC TAAGGAAAAA GCAGTTTAAT
    TGGGAAGATC CTATGCAGAA GGAGCTATTT TTAGCAATGC TGATGACTGC TTGTGATTTA
    TCAGCTATAA CCAAACCGTG GCCAGTTCAG CAACGGATTG CTGAGCTTGT AGCTACTGAG
    TTCTTTGATC AGGGAGATAA AGAGCGGAAG GAACTCAACA TAGAACCAAC AGATCTAATG
    AACAGAGAGA AGAAAAACAA AATTCCAAGC ATGCAGGTTG GATTCATTGA TGCTGTTTGC
    TTGCAGCTGT ATGAGGCCCT AACACATGTA TCAGAGGCCT GCTACCCTTT ACTGGATGGC
    TGTAGAAAAA ACAGGCAGAA ATGGCAGTCC TTAGCTGAAC AACAAGAGAA GAATCTGATT
    AATGGAGAAA GCAATCAAGC CAAACGGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa08195
    Clone ID Related Accession (Same CDS sequence) XM_004941237.2 , XM_004941237.3
    Accession Version XM_004941237.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004941237.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTGCCTT TTGGAGATAA GACGAGAGAA ATGGTTAATG CATGGTTTGC TGAGAGAGTT 
    CATACCATCC CTGTCTGCAA GGAGGGGACC AAGTCTCAGA GTGAATCCTG TGCTTGTCAG
    CAGCTTGCCT GCTCTGAGAA CACCAACTCT GGAACACCAG CAAGGAAAAT CTCTGCTTCA
    GAATTTGACA GACCCTTAAG GCCGATTTTT GTCAAGGACT CAGTAGGAGC AGTAAGCTTC
    CTCTCTGGCT CTGAAAAGAA GGAACAGATG CCTCTGCAAT CTCCAAGGAC TGGGACCAGT
    GCAGGGGATC GGTGCTCAAG GCTTTTAGAA CTTGTGAAAG ACATTTCTAG TCATTTGGAT
    GTCACAGCAC TTTGCCATAA AATTTTCCTG CATATCCATG AGCTTATAGC TGCTGATCGC
    TATTCCCTCT TCTTGGTCTG TGAGGATAGC TCTAACGAGA AGTTTCTTGT AAGCAGGCTG
    TTTGATGTGG CTGAAGGTTC CACCCTAGAA GAAGCTTCAA ATAACTGCAT TCGCCTGGAG
    TGGAACAAGG GCATCGTGGG CCACGTAGCT GCTATAGGCC AGCCTCTGAA CATCAAGAAT
    GCGTATGAGG ATCCAAGATT CAATGCAGAA GTTGACCAGA TCACAGGGTA TAAGACTCAG
    AGCATTCTCT GTATGCCAAT AAAGAATCAT AGGGAAGAGG TTGTTGGTGT GGCTCAAGCA
    ATTAATAAGA AATCTGGAAC TGGTGGGACT TTCACGGAAC AAGATGAAAA GGATTTTGCT
    GCATATTTGG CATTTTGTGG AATTGTTCTT CACAACGCTC AATTGTATGA AACATCTTTG
    CTTGAAAACA GGCGTAATCA GGTGCTTCTT GATCTTGCCA GCCTTATTTT TGAAGAACAA
    CAGTCTTTGG AAGTAATTCT GAAGAAGATA GCTGCCACTA TAATATCCTT CATGCAAGTG
    CAGAGGTGTA CCATCTTCAT AGTGGACGAA GATTGTACTG ATTCATTTTC TAGTGTTTTT
    CATATGGAAG CTGAGGAATT AGAAGATTCA GCAGAAAACC AGAAGAGGGA CTGTGATACA
    AACAAAATCA ATTATATGTA TGCTCAGTAT GTCAAGAATA CAATGGAGCC TCTCAACATC
    CCAGATGTCT GCAAAGACAG AAGATTTCCC TGGACAACTG ACAGTGCAGA AAGTGTAAGT
    CAACATATAA AGAGTTTGCT GTGTACACCA ATAAAAAATG GGAAAAAGAA TAAGGTGATA
    GGGGTTTGCC AGCTTGTGAA TAAGATGGAA GAAAACTCAG GCAAGATCAA GGCTTTCAAC
    AGAAATGATG AGCAGTTTCT GGAAGCATTT GTCATCTTTT GTGGCTTGGG GATCCAGAAT
    ACACAGATGT ATGAAGCTGT AGAAAGAGCA ATGGCCAAAC AGATGGTGAC ATTAGAGGTT
    CTCTCATACC ATGCTTCTGC TGCTGAGGAG GAATCAAGGG AGCTGCAGGT AACAGCGGCT
    GCTGTAGTAC CATCTGCACA ATCTCTCAAC CTAACTGACT TCAACTTCAG TGATTTTGAG
    CTATCAGATT TTGAAACAAC ACTGTGTACA ATTCGAATGT TCACAGACCT CAACCTGGTG
    CAGAATTTCC AAATGAAACA TGAGGTTCTC TGCAGATGGA TTTTAAGCGT GAAGAAAAAT
    TATCGGAAAA ATGTTGCTTA TCACAATTGG AGACATGCCT TTAATACAGC ACAGTGCATG
    TTTGCTGCTT TAAAATCTGG TAAAATTCAG AGCAAACTGA CTGACTTGGA AACGCTGGCT
    TTGCTGATAG CAACTCTAAG CCATGATTTG GATCACAGGG GAGTGAACAA CTCTTACATA
    CAAAGAAGTG AACATCCACT GGCTCAACTG TATTGCCACT CCATCATGGA GCATCACCAT
    TTTGATCAAT GCCTGATGAT CCTGAATAGT CCAGGAAACC AGATTCTCAG CAGCCTTTCA
    ATTGAAGAGT ACAAGGCAAC ACTGAAAATG ATAAAACAAG CCATCCTTGC AACAGACCTA
    GCACTATACA TAAAGAGGAG AGGAGAATTT TTTGAACTTC TAAGGAAAAA GCAGTTTAAT
    TGGGAAGATC CTATGCAGAA GGAGCTATTT TTAGCAATGC TGATGACTGC TTGTGATTTA
    TCAGCTATAA CCAAACCGTG GCCAGTTCAG CAACGGATTG CTGAGCTTGT AGCTACTGAG
    TTCTTTGATC AGGGAGATAA AGAGCGGAAG GAACTCAACA TAGAACCAAC AGATCTAATG
    AACAGAGAGA AGAAAAACAA AATTCCAAGC ATGCAGGTTG GATTCATTGA TGCTGTTTGC
    TTGCAGCTGT ATGAGGCCCT AACACATGTA TCAGAGGCCT GCTACCCTTT ACTGGATGGC
    TGTAGAAAAA ACAGGCAGAA ATGGCAGTCC TTAGCTGAAC AACAAGAGAA GAATCTGATT
    AATGGAGAAA GCAATCAAGC CAAACGGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004941294.1
    CDS2942..5434
    Translation

    Target ORF information:

    RefSeq Version XM_004941237.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus phosphodiesterase 5A (PDE5A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004941237.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTGCCTT TTGGAGATAA GACGAGAGAA ATGGTTAATG CATGGTTTGC TGAGAGAGTT 
    CATACCATCC CTGTCTGCAA GGAGGGGACC AAGTCTCAGA GTGAATCCTG TGCTTGTCAG
    CAGCTTGCCT GCTCTGAGAA CACCAACTCT GGAACACCAG CAAGGAAAAT CTCTGCTTCA
    GAATTTGACA GACCCTTAAG GCCGATTTTT GTCAAGGACT CAGTAGGAGC AGTAAGCTTC
    CTCTCTGGCT CTGAAAAGAA GGAACAGATG CCTCTGCAAT CTCCAAGGAC TGGGACCAGT
    GCAGGGGATC GGTGCTCAAG GCTTTTAGAA CTTGTGAAAG ACATTTCTAG TCATTTGGAT
    GTCACAGCAC TTTGCCATAA AATTTTCCTG CATATCCATG AGCTTATAGC TGCTGATCGC
    TATTCCCTCT TCTTGGTCTG TGAGGATAGC TCTAACGAGA AGTTTCTTGT AAGCAGGCTG
    TTTGATGTGG CTGAAGGTTC CACCCTAGAA GAAGCTTCAA ATAACTGCAT TCGCCTGGAG
    TGGAACAAGG GCATCGTGGG CCACGTAGCT GCTATAGGCC AGCCTCTGAA CATCAAGAAT
    GCGTATGAGG ATCCAAGATT CAATGCAGAA GTTGACCAGA TCACAGGGTA TAAGACTCAG
    AGCATTCTCT GTATGCCAAT AAAGAATCAT AGGGAAGAGG TTGTTGGTGT GGCTCAAGCA
    ATTAATAAGA AATCTGGAAC TGGTGGGACT TTCACGGAAC AAGATGAAAA GGATTTTGCT
    GCATATTTGG CATTTTGTGG AATTGTTCTT CACAACGCTC AATTGTATGA AACATCTTTG
    CTTGAAAACA GGCGTAATCA GGTGCTTCTT GATCTTGCCA GCCTTATTTT TGAAGAACAA
    CAGTCTTTGG AAGTAATTCT GAAGAAGATA GCTGCCACTA TAATATCCTT CATGCAAGTG
    CAGAGGTGTA CCATCTTCAT AGTGGACGAA GATTGTACTG ATTCATTTTC TAGTGTTTTT
    CATATGGAAG CTGAGGAATT AGAAGATTCA GCAGAAAACC AGAAGAGGGA CTGTGATACA
    AACAAAATCA ATTATATGTA TGCTCAGTAT GTCAAGAATA CAATGGAGCC TCTCAACATC
    CCAGATGTCT GCAAAGACAG AAGATTTCCC TGGACAACTG ACAGTGCAGA AAGTGTAAGT
    CAACATATAA AGAGTTTGCT GTGTACACCA ATAAAAAATG GGAAAAAGAA TAAGGTGATA
    GGGGTTTGCC AGCTTGTGAA TAAGATGGAA GAAAACTCAG GCAAGATCAA GGCTTTCAAC
    AGAAATGATG AGCAGTTTCT GGAAGCATTT GTCATCTTTT GTGGCTTGGG GATCCAGAAT
    ACACAGATGT ATGAAGCTGT AGAAAGAGCA ATGGCCAAAC AGATGGTGAC ATTAGAGGTT
    CTCTCATACC ATGCTTCTGC TGCTGAGGAG GAATCAAGGG AGCTGCAGGT AACAGCGGCT
    GCTGTAGTAC CATCTGCACA ATCTCTCAAC CTAACTGACT TCAACTTCAG TGATTTTGAG
    CTATCAGATT TTGAAACAAC ACTGTGTACA ATTCGAATGT TCACAGACCT CAACCTGGTG
    CAGAATTTCC AAATGAAACA TGAGGTTCTC TGCAGATGGA TTTTAAGCGT GAAGAAAAAT
    TATCGGAAAA ATGTTGCTTA TCACAATTGG AGACATGCCT TTAATACAGC ACAGTGCATG
    TTTGCTGCTT TAAAATCTGG TAAAATTCAG AGCAAACTGA CTGACTTGGA AACGCTGGCT
    TTGCTGATAG CAACTCTAAG CCATGATTTG GATCACAGGG GAGTGAACAA CTCTTACATA
    CAAAGAAGTG AACATCCACT GGCTCAACTG TATTGCCACT CCATCATGGA GCATCACCAT
    TTTGATCAAT GCCTGATGAT CCTGAATAGT CCAGGAAACC AGATTCTCAG CAGCCTTTCA
    ATTGAAGAGT ACAAGGCAAC ACTGAAAATG ATAAAACAAG CCATCCTTGC AACAGACCTA
    GCACTATACA TAAAGAGGAG AGGAGAATTT TTTGAACTTC TAAGGAAAAA GCAGTTTAAT
    TGGGAAGATC CTATGCAGAA GGAGCTATTT TTAGCAATGC TGATGACTGC TTGTGATTTA
    TCAGCTATAA CCAAACCGTG GCCAGTTCAG CAACGGATTG CTGAGCTTGT AGCTACTGAG
    TTCTTTGATC AGGGAGATAA AGAGCGGAAG GAACTCAACA TAGAACCAAC AGATCTAATG
    AACAGAGAGA AGAAAAACAA AATTCCAAGC ATGCAGGTTG GATTCATTGA TGCTGTTTGC
    TTGCAGCTGT ATGAGGCCCT AACACATGTA TCAGAGGCCT GCTACCCTTT ACTGGATGGC
    TGTAGAAAAA ACAGGCAGAA ATGGCAGTCC TTAGCTGAAC AACAAGAGAA GAATCTGATT
    AATGGAGAAA GCAATCAAGC CAAACGGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa08196
    Clone ID Related Accession (Same CDS sequence) XM_004941238.2 , XM_004941238.3
    Accession Version XM_004941238.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004941238.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAACTTG CCAGTGATGA AATAGAAATG GTTAATGCAT GGTTTGCTGA GAGAGTTCAT 
    ACCATCCCTG TCTGCAAGGA GGGGACCAAG TCTCAGAGTG AATCCTGTGC TTGTCAGCAG
    CTTGCCTGCT CTGAGAACAC CAACTCTGGA ACACCAGCAA GGAAAATCTC TGCTTCAGAA
    TTTGACAGAC CCTTAAGGCC GATTTTTGTC AAGGACTCAG TAGGAGCAGT AAGCTTCCTC
    TCTGGCTCTG AAAAGAAGGA ACAGATGCCT CTGCAATCTC CAAGGACTGG GACCAGTGCA
    GGGGATCGGT GCTCAAGGCT TTTAGAACTT GTGAAAGACA TTTCTAGTCA TTTGGATGTC
    ACAGCACTTT GCCATAAAAT TTTCCTGCAT ATCCATGAGC TTATAGCTGC TGATCGCTAT
    TCCCTCTTCT TGGTCTGTGA GGATAGCTCT AACGAGAAGT TTCTTGTAAG CAGGCTGTTT
    GATGTGGCTG AAGGTTCCAC CCTAGAAGAA GCTTCAAATA ACTGCATTCG CCTGGAGTGG
    AACAAGGGCA TCGTGGGCCA CGTAGCTGCT ATAGGCCAGC CTCTGAACAT CAAGAATGCG
    TATGAGGATC CAAGATTCAA TGCAGAAGTT GACCAGATCA CAGGGTATAA GACTCAGAGC
    ATTCTCTGTA TGCCAATAAA GAATCATAGG GAAGAGGTTG TTGGTGTGGC TCAAGCAATT
    AATAAGAAAT CTGGAACTGG TGGGACTTTC ACGGAACAAG ATGAAAAGGA TTTTGCTGCA
    TATTTGGCAT TTTGTGGAAT TGTTCTTCAC AACGCTCAAT TGTATGAAAC ATCTTTGCTT
    GAAAACAGGC GTAATCAGGT GCTTCTTGAT CTTGCCAGCC TTATTTTTGA AGAACAACAG
    TCTTTGGAAG TAATTCTGAA GAAGATAGCT GCCACTATAA TATCCTTCAT GCAAGTGCAG
    AGGTGTACCA TCTTCATAGT GGACGAAGAT TGTACTGATT CATTTTCTAG TGTTTTTCAT
    ATGGAAGCTG AGGAATTAGA AGATTCAGCA GAAAACCAGA AGAGGGACTG TGATACAAAC
    AAAATCAATT ATATGTATGC TCAGTATGTC AAGAATACAA TGGAGCCTCT CAACATCCCA
    GATGTCTGCA AAGACAGAAG ATTTCCCTGG ACAACTGACA GTGCAGAAAG TGTAAGTCAA
    CATATAAAGA GTTTGCTGTG TACACCAATA AAAAATGGGA AAAAGAATAA GGTGATAGGG
    GTTTGCCAGC TTGTGAATAA GATGGAAGAA AACTCAGGCA AGATCAAGGC TTTCAACAGA
    AATGATGAGC AGTTTCTGGA AGCATTTGTC ATCTTTTGTG GCTTGGGGAT CCAGAATACA
    CAGATGTATG AAGCTGTAGA AAGAGCAATG GCCAAACAGA TGGTGACATT AGAGGTTCTC
    TCATACCATG CTTCTGCTGC TGAGGAGGAA TCAAGGGAGC TGCAGGTAAC AGCGGCTGCT
    GTAGTACCAT CTGCACAATC TCTCAACCTA ACTGACTTCA ACTTCAGTGA TTTTGAGCTA
    TCAGATTTTG AAACAACACT GTGTACAATT CGAATGTTCA CAGACCTCAA CCTGGTGCAG
    AATTTCCAAA TGAAACATGA GGTTCTCTGC AGATGGATTT TAAGCGTGAA GAAAAATTAT
    CGGAAAAATG TTGCTTATCA CAATTGGAGA CATGCCTTTA ATACAGCACA GTGCATGTTT
    GCTGCTTTAA AATCTGGTAA AATTCAGAGC AAACTGACTG ACTTGGAAAC GCTGGCTTTG
    CTGATAGCAA CTCTAAGCCA TGATTTGGAT CACAGGGGAG TGAACAACTC TTACATACAA
    AGAAGTGAAC ATCCACTGGC TCAACTGTAT TGCCACTCCA TCATGGAGCA TCACCATTTT
    GATCAATGCC TGATGATCCT GAATAGTCCA GGAAACCAGA TTCTCAGCAG CCTTTCAATT
    GAAGAGTACA AGGCAACACT GAAAATGATA AAACAAGCCA TCCTTGCAAC AGACCTAGCA
    CTATACATAA AGAGGAGAGG AGAATTTTTT GAACTTCTAA GGAAAAAGCA GTTTAATTGG
    GAAGATCCTA TGCAGAAGGA GCTATTTTTA GCAATGCTGA TGACTGCTTG TGATTTATCA
    GCTATAACCA AACCGTGGCC AGTTCAGCAA CGGATTGCTG AGCTTGTAGC TACTGAGTTC
    TTTGATCAGG GAGATAAAGA GCGGAAGGAA CTCAACATAG AACCAACAGA TCTAATGAAC
    AGAGAGAAGA AAAACAAAAT TCCAAGCATG CAGGTTGGAT TCATTGATGC TGTTTGCTTG
    CAGCTGTATG AGGCCCTAAC ACATGTATCA GAGGCCTGCT ACCCTTTACT GGATGGCTGT
    AGAAAAAACA GGCAGAAATG GCAGTCCTTA GCTGAACAAC AAGAGAAGAA TCTGATTAAT
    GGAGAAAGCA ATCAAGCCAA ACGGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004941295.1
    CDS219..2708
    Translation

    Target ORF information:

    RefSeq Version XM_004941238.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004941238.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAACTTG CCAGTGATGA AATAGAAATG GTTAATGCAT GGTTTGCTGA GAGAGTTCAT 
    ACCATCCCTG TCTGCAAGGA GGGGACCAAG TCTCAGAGTG AATCCTGTGC TTGTCAGCAG
    CTTGCCTGCT CTGAGAACAC CAACTCTGGA ACACCAGCAA GGAAAATCTC TGCTTCAGAA
    TTTGACAGAC CCTTAAGGCC GATTTTTGTC AAGGACTCAG TAGGAGCAGT AAGCTTCCTC
    TCTGGCTCTG AAAAGAAGGA ACAGATGCCT CTGCAATCTC CAAGGACTGG GACCAGTGCA
    GGGGATCGGT GCTCAAGGCT TTTAGAACTT GTGAAAGACA TTTCTAGTCA TTTGGATGTC
    ACAGCACTTT GCCATAAAAT TTTCCTGCAT ATCCATGAGC TTATAGCTGC TGATCGCTAT
    TCCCTCTTCT TGGTCTGTGA GGATAGCTCT AACGAGAAGT TTCTTGTAAG CAGGCTGTTT
    GATGTGGCTG AAGGTTCCAC CCTAGAAGAA GCTTCAAATA ACTGCATTCG CCTGGAGTGG
    AACAAGGGCA TCGTGGGCCA CGTAGCTGCT ATAGGCCAGC CTCTGAACAT CAAGAATGCG
    TATGAGGATC CAAGATTCAA TGCAGAAGTT GACCAGATCA CAGGGTATAA GACTCAGAGC
    ATTCTCTGTA TGCCAATAAA GAATCATAGG GAAGAGGTTG TTGGTGTGGC TCAAGCAATT
    AATAAGAAAT CTGGAACTGG TGGGACTTTC ACGGAACAAG ATGAAAAGGA TTTTGCTGCA
    TATTTGGCAT TTTGTGGAAT TGTTCTTCAC AACGCTCAAT TGTATGAAAC ATCTTTGCTT
    GAAAACAGGC GTAATCAGGT GCTTCTTGAT CTTGCCAGCC TTATTTTTGA AGAACAACAG
    TCTTTGGAAG TAATTCTGAA GAAGATAGCT GCCACTATAA TATCCTTCAT GCAAGTGCAG
    AGGTGTACCA TCTTCATAGT GGACGAAGAT TGTACTGATT CATTTTCTAG TGTTTTTCAT
    ATGGAAGCTG AGGAATTAGA AGATTCAGCA GAAAACCAGA AGAGGGACTG TGATACAAAC
    AAAATCAATT ATATGTATGC TCAGTATGTC AAGAATACAA TGGAGCCTCT CAACATCCCA
    GATGTCTGCA AAGACAGAAG ATTTCCCTGG ACAACTGACA GTGCAGAAAG TGTAAGTCAA
    CATATAAAGA GTTTGCTGTG TACACCAATA AAAAATGGGA AAAAGAATAA GGTGATAGGG
    GTTTGCCAGC TTGTGAATAA GATGGAAGAA AACTCAGGCA AGATCAAGGC TTTCAACAGA
    AATGATGAGC AGTTTCTGGA AGCATTTGTC ATCTTTTGTG GCTTGGGGAT CCAGAATACA
    CAGATGTATG AAGCTGTAGA AAGAGCAATG GCCAAACAGA TGGTGACATT AGAGGTTCTC
    TCATACCATG CTTCTGCTGC TGAGGAGGAA TCAAGGGAGC TGCAGGTAAC AGCGGCTGCT
    GTAGTACCAT CTGCACAATC TCTCAACCTA ACTGACTTCA ACTTCAGTGA TTTTGAGCTA
    TCAGATTTTG AAACAACACT GTGTACAATT CGAATGTTCA CAGACCTCAA CCTGGTGCAG
    AATTTCCAAA TGAAACATGA GGTTCTCTGC AGATGGATTT TAAGCGTGAA GAAAAATTAT
    CGGAAAAATG TTGCTTATCA CAATTGGAGA CATGCCTTTA ATACAGCACA GTGCATGTTT
    GCTGCTTTAA AATCTGGTAA AATTCAGAGC AAACTGACTG ACTTGGAAAC GCTGGCTTTG
    CTGATAGCAA CTCTAAGCCA TGATTTGGAT CACAGGGGAG TGAACAACTC TTACATACAA
    AGAAGTGAAC ATCCACTGGC TCAACTGTAT TGCCACTCCA TCATGGAGCA TCACCATTTT
    GATCAATGCC TGATGATCCT GAATAGTCCA GGAAACCAGA TTCTCAGCAG CCTTTCAATT
    GAAGAGTACA AGGCAACACT GAAAATGATA AAACAAGCCA TCCTTGCAAC AGACCTAGCA
    CTATACATAA AGAGGAGAGG AGAATTTTTT GAACTTCTAA GGAAAAAGCA GTTTAATTGG
    GAAGATCCTA TGCAGAAGGA GCTATTTTTA GCAATGCTGA TGACTGCTTG TGATTTATCA
    GCTATAACCA AACCGTGGCC AGTTCAGCAA CGGATTGCTG AGCTTGTAGC TACTGAGTTC
    TTTGATCAGG GAGATAAAGA GCGGAAGGAA CTCAACATAG AACCAACAGA TCTAATGAAC
    AGAGAGAAGA AAAACAAAAT TCCAAGCATG CAGGTTGGAT TCATTGATGC TGTTTGCTTG
    CAGCTGTATG AGGCCCTAAC ACATGTATCA GAGGCCTGCT ACCCTTTACT GGATGGCTGT
    AGAAAAAACA GGCAGAAATG GCAGTCCTTA GCTGAACAAC AAGAGAAGAA TCTGATTAAT
    GGAGAAAGCA ATCAAGCCAA ACGGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa08196
    Clone ID Related Accession (Same CDS sequence) XM_004941238.2 , XM_004941238.3
    Accession Version XM_004941238.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004941238.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAACTTG CCAGTGATGA AATAGAAATG GTTAATGCAT GGTTTGCTGA GAGAGTTCAT 
    ACCATCCCTG TCTGCAAGGA GGGGACCAAG TCTCAGAGTG AATCCTGTGC TTGTCAGCAG
    CTTGCCTGCT CTGAGAACAC CAACTCTGGA ACACCAGCAA GGAAAATCTC TGCTTCAGAA
    TTTGACAGAC CCTTAAGGCC GATTTTTGTC AAGGACTCAG TAGGAGCAGT AAGCTTCCTC
    TCTGGCTCTG AAAAGAAGGA ACAGATGCCT CTGCAATCTC CAAGGACTGG GACCAGTGCA
    GGGGATCGGT GCTCAAGGCT TTTAGAACTT GTGAAAGACA TTTCTAGTCA TTTGGATGTC
    ACAGCACTTT GCCATAAAAT TTTCCTGCAT ATCCATGAGC TTATAGCTGC TGATCGCTAT
    TCCCTCTTCT TGGTCTGTGA GGATAGCTCT AACGAGAAGT TTCTTGTAAG CAGGCTGTTT
    GATGTGGCTG AAGGTTCCAC CCTAGAAGAA GCTTCAAATA ACTGCATTCG CCTGGAGTGG
    AACAAGGGCA TCGTGGGCCA CGTAGCTGCT ATAGGCCAGC CTCTGAACAT CAAGAATGCG
    TATGAGGATC CAAGATTCAA TGCAGAAGTT GACCAGATCA CAGGGTATAA GACTCAGAGC
    ATTCTCTGTA TGCCAATAAA GAATCATAGG GAAGAGGTTG TTGGTGTGGC TCAAGCAATT
    AATAAGAAAT CTGGAACTGG TGGGACTTTC ACGGAACAAG ATGAAAAGGA TTTTGCTGCA
    TATTTGGCAT TTTGTGGAAT TGTTCTTCAC AACGCTCAAT TGTATGAAAC ATCTTTGCTT
    GAAAACAGGC GTAATCAGGT GCTTCTTGAT CTTGCCAGCC TTATTTTTGA AGAACAACAG
    TCTTTGGAAG TAATTCTGAA GAAGATAGCT GCCACTATAA TATCCTTCAT GCAAGTGCAG
    AGGTGTACCA TCTTCATAGT GGACGAAGAT TGTACTGATT CATTTTCTAG TGTTTTTCAT
    ATGGAAGCTG AGGAATTAGA AGATTCAGCA GAAAACCAGA AGAGGGACTG TGATACAAAC
    AAAATCAATT ATATGTATGC TCAGTATGTC AAGAATACAA TGGAGCCTCT CAACATCCCA
    GATGTCTGCA AAGACAGAAG ATTTCCCTGG ACAACTGACA GTGCAGAAAG TGTAAGTCAA
    CATATAAAGA GTTTGCTGTG TACACCAATA AAAAATGGGA AAAAGAATAA GGTGATAGGG
    GTTTGCCAGC TTGTGAATAA GATGGAAGAA AACTCAGGCA AGATCAAGGC TTTCAACAGA
    AATGATGAGC AGTTTCTGGA AGCATTTGTC ATCTTTTGTG GCTTGGGGAT CCAGAATACA
    CAGATGTATG AAGCTGTAGA AAGAGCAATG GCCAAACAGA TGGTGACATT AGAGGTTCTC
    TCATACCATG CTTCTGCTGC TGAGGAGGAA TCAAGGGAGC TGCAGGTAAC AGCGGCTGCT
    GTAGTACCAT CTGCACAATC TCTCAACCTA ACTGACTTCA ACTTCAGTGA TTTTGAGCTA
    TCAGATTTTG AAACAACACT GTGTACAATT CGAATGTTCA CAGACCTCAA CCTGGTGCAG
    AATTTCCAAA TGAAACATGA GGTTCTCTGC AGATGGATTT TAAGCGTGAA GAAAAATTAT
    CGGAAAAATG TTGCTTATCA CAATTGGAGA CATGCCTTTA ATACAGCACA GTGCATGTTT
    GCTGCTTTAA AATCTGGTAA AATTCAGAGC AAACTGACTG ACTTGGAAAC GCTGGCTTTG
    CTGATAGCAA CTCTAAGCCA TGATTTGGAT CACAGGGGAG TGAACAACTC TTACATACAA
    AGAAGTGAAC ATCCACTGGC TCAACTGTAT TGCCACTCCA TCATGGAGCA TCACCATTTT
    GATCAATGCC TGATGATCCT GAATAGTCCA GGAAACCAGA TTCTCAGCAG CCTTTCAATT
    GAAGAGTACA AGGCAACACT GAAAATGATA AAACAAGCCA TCCTTGCAAC AGACCTAGCA
    CTATACATAA AGAGGAGAGG AGAATTTTTT GAACTTCTAA GGAAAAAGCA GTTTAATTGG
    GAAGATCCTA TGCAGAAGGA GCTATTTTTA GCAATGCTGA TGACTGCTTG TGATTTATCA
    GCTATAACCA AACCGTGGCC AGTTCAGCAA CGGATTGCTG AGCTTGTAGC TACTGAGTTC
    TTTGATCAGG GAGATAAAGA GCGGAAGGAA CTCAACATAG AACCAACAGA TCTAATGAAC
    AGAGAGAAGA AAAACAAAAT TCCAAGCATG CAGGTTGGAT TCATTGATGC TGTTTGCTTG
    CAGCTGTATG AGGCCCTAAC ACATGTATCA GAGGCCTGCT ACCCTTTACT GGATGGCTGT
    AGAAAAAACA GGCAGAAATG GCAGTCCTTA GCTGAACAAC AAGAGAAGAA TCTGATTAAT
    GGAGAAAGCA ATCAAGCCAA ACGGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004941295.1
    CDS566..3055
    Translation

    Target ORF information:

    RefSeq Version XM_004941238.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus phosphodiesterase 5A (PDE5A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004941238.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAACTTG CCAGTGATGA AATAGAAATG GTTAATGCAT GGTTTGCTGA GAGAGTTCAT 
    ACCATCCCTG TCTGCAAGGA GGGGACCAAG TCTCAGAGTG AATCCTGTGC TTGTCAGCAG
    CTTGCCTGCT CTGAGAACAC CAACTCTGGA ACACCAGCAA GGAAAATCTC TGCTTCAGAA
    TTTGACAGAC CCTTAAGGCC GATTTTTGTC AAGGACTCAG TAGGAGCAGT AAGCTTCCTC
    TCTGGCTCTG AAAAGAAGGA ACAGATGCCT CTGCAATCTC CAAGGACTGG GACCAGTGCA
    GGGGATCGGT GCTCAAGGCT TTTAGAACTT GTGAAAGACA TTTCTAGTCA TTTGGATGTC
    ACAGCACTTT GCCATAAAAT TTTCCTGCAT ATCCATGAGC TTATAGCTGC TGATCGCTAT
    TCCCTCTTCT TGGTCTGTGA GGATAGCTCT AACGAGAAGT TTCTTGTAAG CAGGCTGTTT
    GATGTGGCTG AAGGTTCCAC CCTAGAAGAA GCTTCAAATA ACTGCATTCG CCTGGAGTGG
    AACAAGGGCA TCGTGGGCCA CGTAGCTGCT ATAGGCCAGC CTCTGAACAT CAAGAATGCG
    TATGAGGATC CAAGATTCAA TGCAGAAGTT GACCAGATCA CAGGGTATAA GACTCAGAGC
    ATTCTCTGTA TGCCAATAAA GAATCATAGG GAAGAGGTTG TTGGTGTGGC TCAAGCAATT
    AATAAGAAAT CTGGAACTGG TGGGACTTTC ACGGAACAAG ATGAAAAGGA TTTTGCTGCA
    TATTTGGCAT TTTGTGGAAT TGTTCTTCAC AACGCTCAAT TGTATGAAAC ATCTTTGCTT
    GAAAACAGGC GTAATCAGGT GCTTCTTGAT CTTGCCAGCC TTATTTTTGA AGAACAACAG
    TCTTTGGAAG TAATTCTGAA GAAGATAGCT GCCACTATAA TATCCTTCAT GCAAGTGCAG
    AGGTGTACCA TCTTCATAGT GGACGAAGAT TGTACTGATT CATTTTCTAG TGTTTTTCAT
    ATGGAAGCTG AGGAATTAGA AGATTCAGCA GAAAACCAGA AGAGGGACTG TGATACAAAC
    AAAATCAATT ATATGTATGC TCAGTATGTC AAGAATACAA TGGAGCCTCT CAACATCCCA
    GATGTCTGCA AAGACAGAAG ATTTCCCTGG ACAACTGACA GTGCAGAAAG TGTAAGTCAA
    CATATAAAGA GTTTGCTGTG TACACCAATA AAAAATGGGA AAAAGAATAA GGTGATAGGG
    GTTTGCCAGC TTGTGAATAA GATGGAAGAA AACTCAGGCA AGATCAAGGC TTTCAACAGA
    AATGATGAGC AGTTTCTGGA AGCATTTGTC ATCTTTTGTG GCTTGGGGAT CCAGAATACA
    CAGATGTATG AAGCTGTAGA AAGAGCAATG GCCAAACAGA TGGTGACATT AGAGGTTCTC
    TCATACCATG CTTCTGCTGC TGAGGAGGAA TCAAGGGAGC TGCAGGTAAC AGCGGCTGCT
    GTAGTACCAT CTGCACAATC TCTCAACCTA ACTGACTTCA ACTTCAGTGA TTTTGAGCTA
    TCAGATTTTG AAACAACACT GTGTACAATT CGAATGTTCA CAGACCTCAA CCTGGTGCAG
    AATTTCCAAA TGAAACATGA GGTTCTCTGC AGATGGATTT TAAGCGTGAA GAAAAATTAT
    CGGAAAAATG TTGCTTATCA CAATTGGAGA CATGCCTTTA ATACAGCACA GTGCATGTTT
    GCTGCTTTAA AATCTGGTAA AATTCAGAGC AAACTGACTG ACTTGGAAAC GCTGGCTTTG
    CTGATAGCAA CTCTAAGCCA TGATTTGGAT CACAGGGGAG TGAACAACTC TTACATACAA
    AGAAGTGAAC ATCCACTGGC TCAACTGTAT TGCCACTCCA TCATGGAGCA TCACCATTTT
    GATCAATGCC TGATGATCCT GAATAGTCCA GGAAACCAGA TTCTCAGCAG CCTTTCAATT
    GAAGAGTACA AGGCAACACT GAAAATGATA AAACAAGCCA TCCTTGCAAC AGACCTAGCA
    CTATACATAA AGAGGAGAGG AGAATTTTTT GAACTTCTAA GGAAAAAGCA GTTTAATTGG
    GAAGATCCTA TGCAGAAGGA GCTATTTTTA GCAATGCTGA TGACTGCTTG TGATTTATCA
    GCTATAACCA AACCGTGGCC AGTTCAGCAA CGGATTGCTG AGCTTGTAGC TACTGAGTTC
    TTTGATCAGG GAGATAAAGA GCGGAAGGAA CTCAACATAG AACCAACAGA TCTAATGAAC
    AGAGAGAAGA AAAACAAAAT TCCAAGCATG CAGGTTGGAT TCATTGATGC TGTTTGCTTG
    CAGCTGTATG AGGCCCTAAC ACATGTATCA GAGGCCTGCT ACCCTTTACT GGATGGCTGT
    AGAAAAAACA GGCAGAAATG GCAGTCCTTA GCTGAACAAC AAGAGAAGAA TCTGATTAAT
    GGAGAAAGCA ATCAAGCCAA ACGGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa08197
    Clone ID Related Accession (Same CDS sequence) XM_004941239.2 , XM_004941239.3
    Accession Version XM_004941239.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2475bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004941239.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGGGCGAG AAATGGTTAA TGCATGGTTT GCTGAGAGAG TTCATACCAT CCCTGTCTGC 
    AAGGAGGGGA CCAAGTCTCA GAGTGAATCC TGTGCTTGTC AGCAGCTTGC CTGCTCTGAG
    AACACCAACT CTGGAACACC AGCAAGGAAA ATCTCTGCTT CAGAATTTGA CAGACCCTTA
    AGGCCGATTT TTGTCAAGGA CTCAGTAGGA GCAGTAAGCT TCCTCTCTGG CTCTGAAAAG
    AAGGAACAGA TGCCTCTGCA ATCTCCAAGG ACTGGGACCA GTGCAGGGGA TCGGTGCTCA
    AGGCTTTTAG AACTTGTGAA AGACATTTCT AGTCATTTGG ATGTCACAGC ACTTTGCCAT
    AAAATTTTCC TGCATATCCA TGAGCTTATA GCTGCTGATC GCTATTCCCT CTTCTTGGTC
    TGTGAGGATA GCTCTAACGA GAAGTTTCTT GTAAGCAGGC TGTTTGATGT GGCTGAAGGT
    TCCACCCTAG AAGAAGCTTC AAATAACTGC ATTCGCCTGG AGTGGAACAA GGGCATCGTG
    GGCCACGTAG CTGCTATAGG CCAGCCTCTG AACATCAAGA ATGCGTATGA GGATCCAAGA
    TTCAATGCAG AAGTTGACCA GATCACAGGG TATAAGACTC AGAGCATTCT CTGTATGCCA
    ATAAAGAATC ATAGGGAAGA GGTTGTTGGT GTGGCTCAAG CAATTAATAA GAAATCTGGA
    ACTGGTGGGA CTTTCACGGA ACAAGATGAA AAGGATTTTG CTGCATATTT GGCATTTTGT
    GGAATTGTTC TTCACAACGC TCAATTGTAT GAAACATCTT TGCTTGAAAA CAGGCGTAAT
    CAGGTGCTTC TTGATCTTGC CAGCCTTATT TTTGAAGAAC AACAGTCTTT GGAAGTAATT
    CTGAAGAAGA TAGCTGCCAC TATAATATCC TTCATGCAAG TGCAGAGGTG TACCATCTTC
    ATAGTGGACG AAGATTGTAC TGATTCATTT TCTAGTGTTT TTCATATGGA AGCTGAGGAA
    TTAGAAGATT CAGCAGAAAA CCAGAAGAGG GACTGTGATA CAAACAAAAT CAATTATATG
    TATGCTCAGT ATGTCAAGAA TACAATGGAG CCTCTCAACA TCCCAGATGT CTGCAAAGAC
    AGAAGATTTC CCTGGACAAC TGACAGTGCA GAAAGTGTAA GTCAACATAT AAAGAGTTTG
    CTGTGTACAC CAATAAAAAA TGGGAAAAAG AATAAGGTGA TAGGGGTTTG CCAGCTTGTG
    AATAAGATGG AAGAAAACTC AGGCAAGATC AAGGCTTTCA ACAGAAATGA TGAGCAGTTT
    CTGGAAGCAT TTGTCATCTT TTGTGGCTTG GGGATCCAGA ATACACAGAT GTATGAAGCT
    GTAGAAAGAG CAATGGCCAA ACAGATGGTG ACATTAGAGG TTCTCTCATA CCATGCTTCT
    GCTGCTGAGG AGGAATCAAG GGAGCTGCAG GTAACAGCGG CTGCTGTAGT ACCATCTGCA
    CAATCTCTCA ACCTAACTGA CTTCAACTTC AGTGATTTTG AGCTATCAGA TTTTGAAACA
    ACACTGTGTA CAATTCGAAT GTTCACAGAC CTCAACCTGG TGCAGAATTT CCAAATGAAA
    CATGAGGTTC TCTGCAGATG GATTTTAAGC GTGAAGAAAA ATTATCGGAA AAATGTTGCT
    TATCACAATT GGAGACATGC CTTTAATACA GCACAGTGCA TGTTTGCTGC TTTAAAATCT
    GGTAAAATTC AGAGCAAACT GACTGACTTG GAAACGCTGG CTTTGCTGAT AGCAACTCTA
    AGCCATGATT TGGATCACAG GGGAGTGAAC AACTCTTACA TACAAAGAAG TGAACATCCA
    CTGGCTCAAC TGTATTGCCA CTCCATCATG GAGCATCACC ATTTTGATCA ATGCCTGATG
    ATCCTGAATA GTCCAGGAAA CCAGATTCTC AGCAGCCTTT CAATTGAAGA GTACAAGGCA
    ACACTGAAAA TGATAAAACA AGCCATCCTT GCAACAGACC TAGCACTATA CATAAAGAGG
    AGAGGAGAAT TTTTTGAACT TCTAAGGAAA AAGCAGTTTA ATTGGGAAGA TCCTATGCAG
    AAGGAGCTAT TTTTAGCAAT GCTGATGACT GCTTGTGATT TATCAGCTAT AACCAAACCG
    TGGCCAGTTC AGCAACGGAT TGCTGAGCTT GTAGCTACTG AGTTCTTTGA TCAGGGAGAT
    AAAGAGCGGA AGGAACTCAA CATAGAACCA ACAGATCTAA TGAACAGAGA GAAGAAAAAC
    AAAATTCCAA GCATGCAGGT TGGATTCATT GATGCTGTTT GCTTGCAGCT GTATGAGGCC
    CTAACACATG TATCAGAGGC CTGCTACCCT TTACTGGATG GCTGTAGAAA AAACAGGCAG
    AAATGGCAGT CCTTAGCTGA ACAACAAGAG AAGAATCTGA TTAATGGAGA AAGCAATCAA
    GCCAAACGGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004941296.1
    CDS183..2657
    Translation

    Target ORF information:

    RefSeq Version XM_004941239.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004941239.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGGGCGAG AAATGGTTAA TGCATGGTTT GCTGAGAGAG TTCATACCAT CCCTGTCTGC 
    AAGGAGGGGA CCAAGTCTCA GAGTGAATCC TGTGCTTGTC AGCAGCTTGC CTGCTCTGAG
    AACACCAACT CTGGAACACC AGCAAGGAAA ATCTCTGCTT CAGAATTTGA CAGACCCTTA
    AGGCCGATTT TTGTCAAGGA CTCAGTAGGA GCAGTAAGCT TCCTCTCTGG CTCTGAAAAG
    AAGGAACAGA TGCCTCTGCA ATCTCCAAGG ACTGGGACCA GTGCAGGGGA TCGGTGCTCA
    AGGCTTTTAG AACTTGTGAA AGACATTTCT AGTCATTTGG ATGTCACAGC ACTTTGCCAT
    AAAATTTTCC TGCATATCCA TGAGCTTATA GCTGCTGATC GCTATTCCCT CTTCTTGGTC
    TGTGAGGATA GCTCTAACGA GAAGTTTCTT GTAAGCAGGC TGTTTGATGT GGCTGAAGGT
    TCCACCCTAG AAGAAGCTTC AAATAACTGC ATTCGCCTGG AGTGGAACAA GGGCATCGTG
    GGCCACGTAG CTGCTATAGG CCAGCCTCTG AACATCAAGA ATGCGTATGA GGATCCAAGA
    TTCAATGCAG AAGTTGACCA GATCACAGGG TATAAGACTC AGAGCATTCT CTGTATGCCA
    ATAAAGAATC ATAGGGAAGA GGTTGTTGGT GTGGCTCAAG CAATTAATAA GAAATCTGGA
    ACTGGTGGGA CTTTCACGGA ACAAGATGAA AAGGATTTTG CTGCATATTT GGCATTTTGT
    GGAATTGTTC TTCACAACGC TCAATTGTAT GAAACATCTT TGCTTGAAAA CAGGCGTAAT
    CAGGTGCTTC TTGATCTTGC CAGCCTTATT TTTGAAGAAC AACAGTCTTT GGAAGTAATT
    CTGAAGAAGA TAGCTGCCAC TATAATATCC TTCATGCAAG TGCAGAGGTG TACCATCTTC
    ATAGTGGACG AAGATTGTAC TGATTCATTT TCTAGTGTTT TTCATATGGA AGCTGAGGAA
    TTAGAAGATT CAGCAGAAAA CCAGAAGAGG GACTGTGATA CAAACAAAAT CAATTATATG
    TATGCTCAGT ATGTCAAGAA TACAATGGAG CCTCTCAACA TCCCAGATGT CTGCAAAGAC
    AGAAGATTTC CCTGGACAAC TGACAGTGCA GAAAGTGTAA GTCAACATAT AAAGAGTTTG
    CTGTGTACAC CAATAAAAAA TGGGAAAAAG AATAAGGTGA TAGGGGTTTG CCAGCTTGTG
    AATAAGATGG AAGAAAACTC AGGCAAGATC AAGGCTTTCA ACAGAAATGA TGAGCAGTTT
    CTGGAAGCAT TTGTCATCTT TTGTGGCTTG GGGATCCAGA ATACACAGAT GTATGAAGCT
    GTAGAAAGAG CAATGGCCAA ACAGATGGTG ACATTAGAGG TTCTCTCATA CCATGCTTCT
    GCTGCTGAGG AGGAATCAAG GGAGCTGCAG GTAACAGCGG CTGCTGTAGT ACCATCTGCA
    CAATCTCTCA ACCTAACTGA CTTCAACTTC AGTGATTTTG AGCTATCAGA TTTTGAAACA
    ACACTGTGTA CAATTCGAAT GTTCACAGAC CTCAACCTGG TGCAGAATTT CCAAATGAAA
    CATGAGGTTC TCTGCAGATG GATTTTAAGC GTGAAGAAAA ATTATCGGAA AAATGTTGCT
    TATCACAATT GGAGACATGC CTTTAATACA GCACAGTGCA TGTTTGCTGC TTTAAAATCT
    GGTAAAATTC AGAGCAAACT GACTGACTTG GAAACGCTGG CTTTGCTGAT AGCAACTCTA
    AGCCATGATT TGGATCACAG GGGAGTGAAC AACTCTTACA TACAAAGAAG TGAACATCCA
    CTGGCTCAAC TGTATTGCCA CTCCATCATG GAGCATCACC ATTTTGATCA ATGCCTGATG
    ATCCTGAATA GTCCAGGAAA CCAGATTCTC AGCAGCCTTT CAATTGAAGA GTACAAGGCA
    ACACTGAAAA TGATAAAACA AGCCATCCTT GCAACAGACC TAGCACTATA CATAAAGAGG
    AGAGGAGAAT TTTTTGAACT TCTAAGGAAA AAGCAGTTTA ATTGGGAAGA TCCTATGCAG
    AAGGAGCTAT TTTTAGCAAT GCTGATGACT GCTTGTGATT TATCAGCTAT AACCAAACCG
    TGGCCAGTTC AGCAACGGAT TGCTGAGCTT GTAGCTACTG AGTTCTTTGA TCAGGGAGAT
    AAAGAGCGGA AGGAACTCAA CATAGAACCA ACAGATCTAA TGAACAGAGA GAAGAAAAAC
    AAAATTCCAA GCATGCAGGT TGGATTCATT GATGCTGTTT GCTTGCAGCT GTATGAGGCC
    CTAACACATG TATCAGAGGC CTGCTACCCT TTACTGGATG GCTGTAGAAA AAACAGGCAG
    AAATGGCAGT CCTTAGCTGA ACAACAAGAG AAGAATCTGA TTAATGGAGA AAGCAATCAA
    GCCAAACGGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa08197
    Clone ID Related Accession (Same CDS sequence) XM_004941239.2 , XM_004941239.3
    Accession Version XM_004941239.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2475bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004941239.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGGGCGAG AAATGGTTAA TGCATGGTTT GCTGAGAGAG TTCATACCAT CCCTGTCTGC 
    AAGGAGGGGA CCAAGTCTCA GAGTGAATCC TGTGCTTGTC AGCAGCTTGC CTGCTCTGAG
    AACACCAACT CTGGAACACC AGCAAGGAAA ATCTCTGCTT CAGAATTTGA CAGACCCTTA
    AGGCCGATTT TTGTCAAGGA CTCAGTAGGA GCAGTAAGCT TCCTCTCTGG CTCTGAAAAG
    AAGGAACAGA TGCCTCTGCA ATCTCCAAGG ACTGGGACCA GTGCAGGGGA TCGGTGCTCA
    AGGCTTTTAG AACTTGTGAA AGACATTTCT AGTCATTTGG ATGTCACAGC ACTTTGCCAT
    AAAATTTTCC TGCATATCCA TGAGCTTATA GCTGCTGATC GCTATTCCCT CTTCTTGGTC
    TGTGAGGATA GCTCTAACGA GAAGTTTCTT GTAAGCAGGC TGTTTGATGT GGCTGAAGGT
    TCCACCCTAG AAGAAGCTTC AAATAACTGC ATTCGCCTGG AGTGGAACAA GGGCATCGTG
    GGCCACGTAG CTGCTATAGG CCAGCCTCTG AACATCAAGA ATGCGTATGA GGATCCAAGA
    TTCAATGCAG AAGTTGACCA GATCACAGGG TATAAGACTC AGAGCATTCT CTGTATGCCA
    ATAAAGAATC ATAGGGAAGA GGTTGTTGGT GTGGCTCAAG CAATTAATAA GAAATCTGGA
    ACTGGTGGGA CTTTCACGGA ACAAGATGAA AAGGATTTTG CTGCATATTT GGCATTTTGT
    GGAATTGTTC TTCACAACGC TCAATTGTAT GAAACATCTT TGCTTGAAAA CAGGCGTAAT
    CAGGTGCTTC TTGATCTTGC CAGCCTTATT TTTGAAGAAC AACAGTCTTT GGAAGTAATT
    CTGAAGAAGA TAGCTGCCAC TATAATATCC TTCATGCAAG TGCAGAGGTG TACCATCTTC
    ATAGTGGACG AAGATTGTAC TGATTCATTT TCTAGTGTTT TTCATATGGA AGCTGAGGAA
    TTAGAAGATT CAGCAGAAAA CCAGAAGAGG GACTGTGATA CAAACAAAAT CAATTATATG
    TATGCTCAGT ATGTCAAGAA TACAATGGAG CCTCTCAACA TCCCAGATGT CTGCAAAGAC
    AGAAGATTTC CCTGGACAAC TGACAGTGCA GAAAGTGTAA GTCAACATAT AAAGAGTTTG
    CTGTGTACAC CAATAAAAAA TGGGAAAAAG AATAAGGTGA TAGGGGTTTG CCAGCTTGTG
    AATAAGATGG AAGAAAACTC AGGCAAGATC AAGGCTTTCA ACAGAAATGA TGAGCAGTTT
    CTGGAAGCAT TTGTCATCTT TTGTGGCTTG GGGATCCAGA ATACACAGAT GTATGAAGCT
    GTAGAAAGAG CAATGGCCAA ACAGATGGTG ACATTAGAGG TTCTCTCATA CCATGCTTCT
    GCTGCTGAGG AGGAATCAAG GGAGCTGCAG GTAACAGCGG CTGCTGTAGT ACCATCTGCA
    CAATCTCTCA ACCTAACTGA CTTCAACTTC AGTGATTTTG AGCTATCAGA TTTTGAAACA
    ACACTGTGTA CAATTCGAAT GTTCACAGAC CTCAACCTGG TGCAGAATTT CCAAATGAAA
    CATGAGGTTC TCTGCAGATG GATTTTAAGC GTGAAGAAAA ATTATCGGAA AAATGTTGCT
    TATCACAATT GGAGACATGC CTTTAATACA GCACAGTGCA TGTTTGCTGC TTTAAAATCT
    GGTAAAATTC AGAGCAAACT GACTGACTTG GAAACGCTGG CTTTGCTGAT AGCAACTCTA
    AGCCATGATT TGGATCACAG GGGAGTGAAC AACTCTTACA TACAAAGAAG TGAACATCCA
    CTGGCTCAAC TGTATTGCCA CTCCATCATG GAGCATCACC ATTTTGATCA ATGCCTGATG
    ATCCTGAATA GTCCAGGAAA CCAGATTCTC AGCAGCCTTT CAATTGAAGA GTACAAGGCA
    ACACTGAAAA TGATAAAACA AGCCATCCTT GCAACAGACC TAGCACTATA CATAAAGAGG
    AGAGGAGAAT TTTTTGAACT TCTAAGGAAA AAGCAGTTTA ATTGGGAAGA TCCTATGCAG
    AAGGAGCTAT TTTTAGCAAT GCTGATGACT GCTTGTGATT TATCAGCTAT AACCAAACCG
    TGGCCAGTTC AGCAACGGAT TGCTGAGCTT GTAGCTACTG AGTTCTTTGA TCAGGGAGAT
    AAAGAGCGGA AGGAACTCAA CATAGAACCA ACAGATCTAA TGAACAGAGA GAAGAAAAAC
    AAAATTCCAA GCATGCAGGT TGGATTCATT GATGCTGTTT GCTTGCAGCT GTATGAGGCC
    CTAACACATG TATCAGAGGC CTGCTACCCT TTACTGGATG GCTGTAGAAA AAACAGGCAG
    AAATGGCAGT CCTTAGCTGA ACAACAAGAG AAGAATCTGA TTAATGGAGA AAGCAATCAA
    GCCAAACGGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004941296.1
    CDS178..2652
    Translation

    Target ORF information:

    RefSeq Version XM_004941239.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus phosphodiesterase 5A (PDE5A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004941239.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGGGCGAG AAATGGTTAA TGCATGGTTT GCTGAGAGAG TTCATACCAT CCCTGTCTGC 
    AAGGAGGGGA CCAAGTCTCA GAGTGAATCC TGTGCTTGTC AGCAGCTTGC CTGCTCTGAG
    AACACCAACT CTGGAACACC AGCAAGGAAA ATCTCTGCTT CAGAATTTGA CAGACCCTTA
    AGGCCGATTT TTGTCAAGGA CTCAGTAGGA GCAGTAAGCT TCCTCTCTGG CTCTGAAAAG
    AAGGAACAGA TGCCTCTGCA ATCTCCAAGG ACTGGGACCA GTGCAGGGGA TCGGTGCTCA
    AGGCTTTTAG AACTTGTGAA AGACATTTCT AGTCATTTGG ATGTCACAGC ACTTTGCCAT
    AAAATTTTCC TGCATATCCA TGAGCTTATA GCTGCTGATC GCTATTCCCT CTTCTTGGTC
    TGTGAGGATA GCTCTAACGA GAAGTTTCTT GTAAGCAGGC TGTTTGATGT GGCTGAAGGT
    TCCACCCTAG AAGAAGCTTC AAATAACTGC ATTCGCCTGG AGTGGAACAA GGGCATCGTG
    GGCCACGTAG CTGCTATAGG CCAGCCTCTG AACATCAAGA ATGCGTATGA GGATCCAAGA
    TTCAATGCAG AAGTTGACCA GATCACAGGG TATAAGACTC AGAGCATTCT CTGTATGCCA
    ATAAAGAATC ATAGGGAAGA GGTTGTTGGT GTGGCTCAAG CAATTAATAA GAAATCTGGA
    ACTGGTGGGA CTTTCACGGA ACAAGATGAA AAGGATTTTG CTGCATATTT GGCATTTTGT
    GGAATTGTTC TTCACAACGC TCAATTGTAT GAAACATCTT TGCTTGAAAA CAGGCGTAAT
    CAGGTGCTTC TTGATCTTGC CAGCCTTATT TTTGAAGAAC AACAGTCTTT GGAAGTAATT
    CTGAAGAAGA TAGCTGCCAC TATAATATCC TTCATGCAAG TGCAGAGGTG TACCATCTTC
    ATAGTGGACG AAGATTGTAC TGATTCATTT TCTAGTGTTT TTCATATGGA AGCTGAGGAA
    TTAGAAGATT CAGCAGAAAA CCAGAAGAGG GACTGTGATA CAAACAAAAT CAATTATATG
    TATGCTCAGT ATGTCAAGAA TACAATGGAG CCTCTCAACA TCCCAGATGT CTGCAAAGAC
    AGAAGATTTC CCTGGACAAC TGACAGTGCA GAAAGTGTAA GTCAACATAT AAAGAGTTTG
    CTGTGTACAC CAATAAAAAA TGGGAAAAAG AATAAGGTGA TAGGGGTTTG CCAGCTTGTG
    AATAAGATGG AAGAAAACTC AGGCAAGATC AAGGCTTTCA ACAGAAATGA TGAGCAGTTT
    CTGGAAGCAT TTGTCATCTT TTGTGGCTTG GGGATCCAGA ATACACAGAT GTATGAAGCT
    GTAGAAAGAG CAATGGCCAA ACAGATGGTG ACATTAGAGG TTCTCTCATA CCATGCTTCT
    GCTGCTGAGG AGGAATCAAG GGAGCTGCAG GTAACAGCGG CTGCTGTAGT ACCATCTGCA
    CAATCTCTCA ACCTAACTGA CTTCAACTTC AGTGATTTTG AGCTATCAGA TTTTGAAACA
    ACACTGTGTA CAATTCGAAT GTTCACAGAC CTCAACCTGG TGCAGAATTT CCAAATGAAA
    CATGAGGTTC TCTGCAGATG GATTTTAAGC GTGAAGAAAA ATTATCGGAA AAATGTTGCT
    TATCACAATT GGAGACATGC CTTTAATACA GCACAGTGCA TGTTTGCTGC TTTAAAATCT
    GGTAAAATTC AGAGCAAACT GACTGACTTG GAAACGCTGG CTTTGCTGAT AGCAACTCTA
    AGCCATGATT TGGATCACAG GGGAGTGAAC AACTCTTACA TACAAAGAAG TGAACATCCA
    CTGGCTCAAC TGTATTGCCA CTCCATCATG GAGCATCACC ATTTTGATCA ATGCCTGATG
    ATCCTGAATA GTCCAGGAAA CCAGATTCTC AGCAGCCTTT CAATTGAAGA GTACAAGGCA
    ACACTGAAAA TGATAAAACA AGCCATCCTT GCAACAGACC TAGCACTATA CATAAAGAGG
    AGAGGAGAAT TTTTTGAACT TCTAAGGAAA AAGCAGTTTA ATTGGGAAGA TCCTATGCAG
    AAGGAGCTAT TTTTAGCAAT GCTGATGACT GCTTGTGATT TATCAGCTAT AACCAAACCG
    TGGCCAGTTC AGCAACGGAT TGCTGAGCTT GTAGCTACTG AGTTCTTTGA TCAGGGAGAT
    AAAGAGCGGA AGGAACTCAA CATAGAACCA ACAGATCTAA TGAACAGAGA GAAGAAAAAC
    AAAATTCCAA GCATGCAGGT TGGATTCATT GATGCTGTTT GCTTGCAGCT GTATGAGGCC
    CTAACACATG TATCAGAGGC CTGCTACCCT TTACTGGATG GCTGTAGAAA AAACAGGCAG
    AAATGGCAGT CCTTAGCTGA ACAACAAGAG AAGAATCTGA TTAATGGAGA AAGCAATCAA
    GCCAAACGGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa62936
    Clone ID Related Accession (Same CDS sequence) NM_001319020.1
    Accession Version NM_001319020.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-25
    Organism Gallus gallus(chicken)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JQ405664.1. On Jan 20, 2016 this sequence version replaced XM_015276369.1. ##Evidence-Data-START## Transcript exon combination :: JQ405664.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCTTC CAGAAGTGGT GTTTACTGGG AACCTTTGCT GGAATTCAGA GATTGCACAA 
    TTTGAATATA TCAATTACAT TTATTACTTT GAAAATAAGT ACAGTTTTGT TTTCTTCTTC
    TTGATAGTTG GTGGACAAGA AATGGTTAAT GCATGGTTTG CTGAGAGAGT TCATACCATC
    CCTGTCTGCA AGGAGGGGAC CAAGTCTCAG AGTGAATCCT GTGCTTGTCA GCAGCTTGCC
    TGCTCTGAGA ACACCAACTC TGGAACACCA GCAAGGAAAA TCTCTGCTTC AGAATTTGAC
    AGACCCTTAA GGCCGATTTT TGTCAAGGAC TCAGTAGGAG CAGTAAGCTT CCTCTCTGGC
    TCTGAAAAGA AGGAACAGAT GCCTCTGCAA TCTCCAAGGA CTGGGACCAG TGCAGGGGAT
    CGGTGCTCAA GGCTTTTAGA ACTTGTGAAA GACATTTCTA GTCATTTGGA TGTCACAGCA
    CTTTGCCATA AAATTTTCCT GCATATCCAT GAGCTTATAG CTGCTGATCG CTATTCCCTC
    TTCTTGGTCT GTGAGGATAG CTCTAACGAG AAGTTTCTTG TAAGCAGGCT GTTTGATGTG
    GCTGAAGGTT CCACCCTAGA AGAAGCTTCA AATAACTGCA TTCGCCTGGA GTGGAACAAG
    GGCATCGTGG GCCACGTAGC TGCTATAGGC CAGCCTCTGA ACATCAAGAA TGCGTATGAG
    GATCCAAGAT TCAATGCAGA AGTTGACCAG ATCACAGGGT ATAAGACTCA GAGCATTCTC
    TGTATGCCAA TAAAGAATCA TAGGGAAGAG GTTGTTGGTG TGGCTCAAGC AATTAATAAG
    AAATCTGGAA CTGGTGGGAC TTTCACGGAA CAAGATGAAA AGGATTTTGC TGCATATTTG
    GCATTTTGTG GAATTGTTCT TCACAACGCT CAATTGTATG AAACATCTTT GCTTGAAAAC
    AGGCGTAATC AGGTGCTTCT TGATCTTGCC AGCCTTATTT TTGAAGAACA ACAGTCTTTG
    GAAGTAATTC TGAAGAAGAT AGCTGCCACT ATAATATCCT TCATGCAAGT GCAGAGGTGT
    ACCATCTTCA TAGTGGACGA AGATTGTACT GATTCATTTT CTAGTGTTTT TCATATGGAA
    GCTGAGGAAT TAGAAGATTC AGCAGAAAAC CAGAAGAGGG ACTGTGATAC AAACAAAATC
    AATTATATGT ATGCTCAGTA TGTCAAGAAT ACAATGGAGC CTCTCAACAT CCCAGATGTC
    TGCAAAGACA GAAGATTTCC CTGGACAACT GACAGTGCAG AAAGTGTAAG TCAACATATA
    AAGAGTTTGC TGTGTACACC AATAAAAAAT GGGAAAAAGA ATAAGGTGAT AGGGGTTTGC
    CAGCTTGTGA ATAAGATGGA AGAAAACTCA GGCAAGATCA AGGCTTTCAA CAGAAATGAT
    GAGCAGTTTC TGGAAGCATT TGTCATCTTT TGTGGCTTGG GGATCCAGAA TACACAGATG
    TATGAAGCTG TAGAAAGAGC AATGGCCAAA CAGATGGTGA CATTAGAGGT TCTCTCATAC
    CATGCTTCTG CTGCTGAGGA GGAATCAAGG GAGCTGCAGG TAACAGCGGC TGCTGTAGTA
    CCATCTGCAC AATCTCTCAA CCTAACTGAC TTCAACTTCA GTGATTTTGA GCTATCAGAT
    TTTGAAACAA CACTGTGTAC AATTCGAATG TTCACAGACC TCAACCTGGT GCAGAATTTC
    CAAATGAAAC ATGAGGTTCT CTGCAGATGG ATTTTAAGCG TGAAGAAAAA TTATCGGAAA
    AATGTTGCTT ATCACAATTG GAGACATGCC TTTAATACAG CACAGTGCAT GTTTGCTGCT
    TTAAAATCTG GTAAAATTCA GAGCAAACTG ACTGACTTGG AAACGCTGGC TTTGCTGATA
    GCAACTCTAA GCCATGATTT GGATCACAGG GGAGTGAACA ACTCTTACAT ACAAAGAAGT
    GAACATCCAC TGGCTCAACT GTATTGCCAC TCCATCATGG AGCATCACCA TTTTGATCAA
    TGCCTGATGA TCCTGAATAG TCCAGGAAAC CAGATTCTCA GCAGCCTTTC AATTGAAGAA
    TACAAGGCAA CACTGAAAAT GATAAAACAA GCCATCCTTG CAACAGACCT AGCACTATAC
    ATAAAGAGGA GAGGAGAATT TTTTGAACTT CTAAGGAAAA AGCAGTTTAA TTGGGAAGAT
    CCTATGCAGA AGGAGCTATT TTTAGCAATG CTGATGACTG CTTGTGATTT ATCAGCTATA
    ACCAAACCGT GGCCAGTTCA GCAACGGATT GCTGAGCTTG TAGCTACTGA GTTCTTTGAT
    CAGGGAGATA AAGAGCGGAA GGAACTCAAC ATAGAACCAA CAGATCTAAT GAACAGAGAG
    AAGAAAAACA AAATTCCAAG CATGCAGGTT GGATTCATTG ATGCTGTTTG CTTGCAGCTG
    TATGAGGCCC TAACACATGT ATCAGAGGCC TGCTACCCTT TACTGGATGG CTGTAGAAAA
    AACAGGCAGA AATGGCAGTC CTTAGCTGAA CAACAAGAGA AGAATCTGAT TAATGGAGAA
    AGCAATCAAG CCAAACGGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001305949.1
    CDS1..2604
    Translation

    Target ORF information:

    RefSeq Version NM_001319020.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus phosphodiesterase 5A (PDE5A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001319020.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCTTC CAGAAGTGGT GTTTACTGGG AACCTTTGCT GGAATTCAGA GATTGCACAA 
    TTTGAATATA TCAATTACAT TTATTACTTT GAAAATAAGT ACAGTTTTGT TTTCTTCTTC
    TTGATAGTTG GTGGACAAGA AATGGTTAAT GCATGGTTTG CTGAGAGAGT TCATACCATC
    CCTGTCTGCA AGGAGGGGAC CAAGTCTCAG AGTGAATCCT GTGCTTGTCA GCAGCTTGCC
    TGCTCTGAGA ACACCAACTC TGGAACACCA GCAAGGAAAA TCTCTGCTTC AGAATTTGAC
    AGACCCTTAA GGCCGATTTT TGTCAAGGAC TCAGTAGGAG CAGTAAGCTT CCTCTCTGGC
    TCTGAAAAGA AGGAACAGAT GCCTCTGCAA TCTCCAAGGA CTGGGACCAG TGCAGGGGAT
    CGGTGCTCAA GGCTTTTAGA ACTTGTGAAA GACATTTCTA GTCATTTGGA TGTCACAGCA
    CTTTGCCATA AAATTTTCCT GCATATCCAT GAGCTTATAG CTGCTGATCG CTATTCCCTC
    TTCTTGGTCT GTGAGGATAG CTCTAACGAG AAGTTTCTTG TAAGCAGGCT GTTTGATGTG
    GCTGAAGGTT CCACCCTAGA AGAAGCTTCA AATAACTGCA TTCGCCTGGA GTGGAACAAG
    GGCATCGTGG GCCACGTAGC TGCTATAGGC CAGCCTCTGA ACATCAAGAA TGCGTATGAG
    GATCCAAGAT TCAATGCAGA AGTTGACCAG ATCACAGGGT ATAAGACTCA GAGCATTCTC
    TGTATGCCAA TAAAGAATCA TAGGGAAGAG GTTGTTGGTG TGGCTCAAGC AATTAATAAG
    AAATCTGGAA CTGGTGGGAC TTTCACGGAA CAAGATGAAA AGGATTTTGC TGCATATTTG
    GCATTTTGTG GAATTGTTCT TCACAACGCT CAATTGTATG AAACATCTTT GCTTGAAAAC
    AGGCGTAATC AGGTGCTTCT TGATCTTGCC AGCCTTATTT TTGAAGAACA ACAGTCTTTG
    GAAGTAATTC TGAAGAAGAT AGCTGCCACT ATAATATCCT TCATGCAAGT GCAGAGGTGT
    ACCATCTTCA TAGTGGACGA AGATTGTACT GATTCATTTT CTAGTGTTTT TCATATGGAA
    GCTGAGGAAT TAGAAGATTC AGCAGAAAAC CAGAAGAGGG ACTGTGATAC AAACAAAATC
    AATTATATGT ATGCTCAGTA TGTCAAGAAT ACAATGGAGC CTCTCAACAT CCCAGATGTC
    TGCAAAGACA GAAGATTTCC CTGGACAACT GACAGTGCAG AAAGTGTAAG TCAACATATA
    AAGAGTTTGC TGTGTACACC AATAAAAAAT GGGAAAAAGA ATAAGGTGAT AGGGGTTTGC
    CAGCTTGTGA ATAAGATGGA AGAAAACTCA GGCAAGATCA AGGCTTTCAA CAGAAATGAT
    GAGCAGTTTC TGGAAGCATT TGTCATCTTT TGTGGCTTGG GGATCCAGAA TACACAGATG
    TATGAAGCTG TAGAAAGAGC AATGGCCAAA CAGATGGTGA CATTAGAGGT TCTCTCATAC
    CATGCTTCTG CTGCTGAGGA GGAATCAAGG GAGCTGCAGG TAACAGCGGC TGCTGTAGTA
    CCATCTGCAC AATCTCTCAA CCTAACTGAC TTCAACTTCA GTGATTTTGA GCTATCAGAT
    TTTGAAACAA CACTGTGTAC AATTCGAATG TTCACAGACC TCAACCTGGT GCAGAATTTC
    CAAATGAAAC ATGAGGTTCT CTGCAGATGG ATTTTAAGCG TGAAGAAAAA TTATCGGAAA
    AATGTTGCTT ATCACAATTG GAGACATGCC TTTAATACAG CACAGTGCAT GTTTGCTGCT
    TTAAAATCTG GTAAAATTCA GAGCAAACTG ACTGACTTGG AAACGCTGGC TTTGCTGATA
    GCAACTCTAA GCCATGATTT GGATCACAGG GGAGTGAACA ACTCTTACAT ACAAAGAAGT
    GAACATCCAC TGGCTCAACT GTATTGCCAC TCCATCATGG AGCATCACCA TTTTGATCAA
    TGCCTGATGA TCCTGAATAG TCCAGGAAAC CAGATTCTCA GCAGCCTTTC AATTGAAGAA
    TACAAGGCAA CACTGAAAAT GATAAAACAA GCCATCCTTG CAACAGACCT AGCACTATAC
    ATAAAGAGGA GAGGAGAATT TTTTGAACTT CTAAGGAAAA AGCAGTTTAA TTGGGAAGAT
    CCTATGCAGA AGGAGCTATT TTTAGCAATG CTGATGACTG CTTGTGATTT ATCAGCTATA
    ACCAAACCGT GGCCAGTTCA GCAACGGATT GCTGAGCTTG TAGCTACTGA GTTCTTTGAT
    CAGGGAGATA AAGAGCGGAA GGAACTCAAC ATAGAACCAA CAGATCTAAT GAACAGAGAG
    AAGAAAAACA AAATTCCAAG CATGCAGGTT GGATTCATTG ATGCTGTTTG CTTGCAGCTG
    TATGAGGCCC TAACACATGT ATCAGAGGCC TGCTACCCTT TACTGGATGG CTGTAGAAAA
    AACAGGCAGA AATGGCAGTC CTTAGCTGAA CAACAAGAGA AGAATCTGAT TAATGGAGAA
    AGCAATCAAG CCAAACGGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35174
    Clone ID Related Accession (Same CDS sequence) XM_025149655.1 , XM_015276368.1 , XM_015276368.2
    Accession Version XM_025149655.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTTAATG CATGGTTTGC TGAGAGAGTT CATACCATCC CTGTCTGCAA GGAGGGGACC 
    AAGTCTCAGA GTGAATCCTG TGCTTGTCAG CAGCTTGCCT GCTCTGAGAA CACCAACTCT
    GGAACACCAG CAAGGAAAAT CTCTGCTTCA GAATTTGACA GACCCTTAAG GCCGATTTTT
    GTCAAGGACT CAGTAGGAGC AGTAAGCTTC CTCTCTGGCT CTGAAAAGAA GGAACAGATG
    CCTCTGCAAT CTCCAAGGAC TGGGACCAGT GCAGGGGATC GGTGCTCAAG GCTTTTAGAA
    CTTGTGAAAG ACATTTCTAG TCATTTGGAT GTCACAGCAC TTTGCCATAA AATTTTCCTG
    CATATCCATG AGCTTATAGC TGCTGATCGC TATTCCCTCT TCTTGGTCTG TGAGGATAGC
    TCTAACGAGA AGTTTCTTGT AAGCAGGCTG TTTGATGTGG CTGAAGGTTC CACCCTAGAA
    GAAGCTTCAA ATAACTGCAT TCGCCTGGAG TGGAACAAGG GCATCGTGGG CCACGTAGCT
    GCTATAGGCC AGCCTCTGAA CATCAAGAAT GCGTATGAGG ATCCAAGATT CAATGCAGAA
    GTTGACCAGA TCACAGGGTA TAAGACTCAG AGCATTCTCT GTATGCCAAT AAAGAATCAT
    AGGGAAGAGG TTGTTGGTGT GGCTCAAGCA ATTAATAAGA AATCTGGAAC TGGTGGGACT
    TTCACGGAAC AAGATGAAAA GGATTTTGCT GCATATTTGG CATTTTGTGG AATTGTTCTT
    CACAACGCTC AATTGTATGA AACATCTTTG CTTGAAAACA GGCGTAATCA GGTGCTTCTT
    GATCTTGCCA GCCTTATTTT TGAAGAACAA CAGTCTTTGG AAGTAATTCT GAAGAAGATA
    GCTGCCACTA TAATATCCTT CATGCAAGTG CAGAGGTGTA CCATCTTCAT AGTGGACGAA
    GATTGTACTG ATTCATTTTC TAGTGTTTTT CATATGGAAG CTGAGGAATT AGAAGATTCA
    GCAGAAAACC AGAAGAGGGA CTGTGATACA AACAAAATCA ATTATATGTA TGCTCAGTAT
    GTCAAGAATA CAATGGAGCC TCTCAACATC CCAGATGTCT GCAAAGACAG AAGATTTCCC
    TGGACAACTG ACAGTGCAGA AAGTGTAAGT CAACATATAA AGAGTTTGCT GTGTACACCA
    ATAAAAAATG GGAAAAAGAA TAAGGTGATA GGGGTTTGCC AGCTTGTGAA TAAGATGGAA
    GAAAACTCAG GCAAGATCAA GGCTTTCAAC AGAAATGATG AGCAGTTTCT GGAAGCATTT
    GTCATCTTTT GTGGCTTGGG GATCCAGAAT ACACAGATGT ATGAAGCTGT AGAAAGAGCA
    ATGGCCAAAC AGATGGTGAC ATTAGAGGTT CTCTCATACC ATGCTTCTGC TGCTGAGGAG
    GAATCAAGGG AGCTGCAGGT AACAGCGGCT GCTGTAGTAC CATCTGCACA ATCTCTCAAC
    CTAACTGACT TCAACTTCAG TGATTTTGAG CTATCAGATT TTGAAACAAC ACTGTGTACA
    ATTCGAATGT TCACAGACCT CAACCTGGTG CAGAATTTCC AAATGAAACA TGAGGTTCTC
    TGCAGATGGA TTTTAAGCGT GAAGAAAAAT TATCGGAAAA ATGTTGCTTA TCACAATTGG
    AGACATGCCT TTAATACAGC ACAGTGCATG TTTGCTGCTT TAAAATCTGG TAAAATTCAG
    AGCAAACTGA CTGACTTGGA AACGCTGGCT TTGCTGATAG CAACTCTAAG CCATGATTTG
    GATCACAGGG GAGTGAACAA CTCTTACATA CAAAGAAGTG AACATCCACT GGCTCAACTG
    TATTGCCACT CCATCATGGA GCATCACCAT TTTGATCAAT GCCTGATGAT CCTGAATAGT
    CCAGGAAACC AGATTCTCAG CAGCCTTTCA ATTGAAGAGT ACAAGGCAAC ACTGAAAATG
    ATAAAACAAG CCATCCTTGC AACAGACCTA GCACTATACA TAAAGAGGAG AGGAGAATTT
    TTTGAACTTC TAAGGAAAAA GCAGTTTAAT TGGGAAGATC CTATGCAGAA GGAGCTATTT
    TTAGCAATGC TGATGACTGC TTGTGATTTA TCAGCTATAA CCAAACCGTG GCCAGTTCAG
    CAACGGATTG CTGAGCTTGT AGCTACTGAG TTCTTTGATC AGGGAGATAA AGAGCGGAAG
    GAACTCAACA TAGAACCAAC AGATCTAATG AACAGAGAGA AGAAAAACAA AATTCCAAGC
    ATGCAGGTTG GATTCATTGA TGCTGTTTGC TTGCAGCTGT ATGAGGCCCT AACACATGTA
    TCAGAGGCCT GCTACCCTTT ACTGGATGGC TGTAGAAAAA ACAGGCAGAA ATGGCAGTCC
    TTAGCTGAAC AACAAGAGAA GAATCTGATT AATGGAGAAA GCAATCAAGC CAAACGGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005423.1
    CDS137..2599
    Translation

    Target ORF information:

    RefSeq Version XM_025149655.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus phosphodiesterase 5A (PDE5A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149655.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTTAATG CATGGTTTGC TGAGAGAGTT CATACCATCC CTGTCTGCAA GGAGGGGACC 
    AAGTCTCAGA GTGAATCCTG TGCTTGTCAG CAGCTTGCCT GCTCTGAGAA CACCAACTCT
    GGAACACCAG CAAGGAAAAT CTCTGCTTCA GAATTTGACA GACCCTTAAG GCCGATTTTT
    GTCAAGGACT CAGTAGGAGC AGTAAGCTTC CTCTCTGGCT CTGAAAAGAA GGAACAGATG
    CCTCTGCAAT CTCCAAGGAC TGGGACCAGT GCAGGGGATC GGTGCTCAAG GCTTTTAGAA
    CTTGTGAAAG ACATTTCTAG TCATTTGGAT GTCACAGCAC TTTGCCATAA AATTTTCCTG
    CATATCCATG AGCTTATAGC TGCTGATCGC TATTCCCTCT TCTTGGTCTG TGAGGATAGC
    TCTAACGAGA AGTTTCTTGT AAGCAGGCTG TTTGATGTGG CTGAAGGTTC CACCCTAGAA
    GAAGCTTCAA ATAACTGCAT TCGCCTGGAG TGGAACAAGG GCATCGTGGG CCACGTAGCT
    GCTATAGGCC AGCCTCTGAA CATCAAGAAT GCGTATGAGG ATCCAAGATT CAATGCAGAA
    GTTGACCAGA TCACAGGGTA TAAGACTCAG AGCATTCTCT GTATGCCAAT AAAGAATCAT
    AGGGAAGAGG TTGTTGGTGT GGCTCAAGCA ATTAATAAGA AATCTGGAAC TGGTGGGACT
    TTCACGGAAC AAGATGAAAA GGATTTTGCT GCATATTTGG CATTTTGTGG AATTGTTCTT
    CACAACGCTC AATTGTATGA AACATCTTTG CTTGAAAACA GGCGTAATCA GGTGCTTCTT
    GATCTTGCCA GCCTTATTTT TGAAGAACAA CAGTCTTTGG AAGTAATTCT GAAGAAGATA
    GCTGCCACTA TAATATCCTT CATGCAAGTG CAGAGGTGTA CCATCTTCAT AGTGGACGAA
    GATTGTACTG ATTCATTTTC TAGTGTTTTT CATATGGAAG CTGAGGAATT AGAAGATTCA
    GCAGAAAACC AGAAGAGGGA CTGTGATACA AACAAAATCA ATTATATGTA TGCTCAGTAT
    GTCAAGAATA CAATGGAGCC TCTCAACATC CCAGATGTCT GCAAAGACAG AAGATTTCCC
    TGGACAACTG ACAGTGCAGA AAGTGTAAGT CAACATATAA AGAGTTTGCT GTGTACACCA
    ATAAAAAATG GGAAAAAGAA TAAGGTGATA GGGGTTTGCC AGCTTGTGAA TAAGATGGAA
    GAAAACTCAG GCAAGATCAA GGCTTTCAAC AGAAATGATG AGCAGTTTCT GGAAGCATTT
    GTCATCTTTT GTGGCTTGGG GATCCAGAAT ACACAGATGT ATGAAGCTGT AGAAAGAGCA
    ATGGCCAAAC AGATGGTGAC ATTAGAGGTT CTCTCATACC ATGCTTCTGC TGCTGAGGAG
    GAATCAAGGG AGCTGCAGGT AACAGCGGCT GCTGTAGTAC CATCTGCACA ATCTCTCAAC
    CTAACTGACT TCAACTTCAG TGATTTTGAG CTATCAGATT TTGAAACAAC ACTGTGTACA
    ATTCGAATGT TCACAGACCT CAACCTGGTG CAGAATTTCC AAATGAAACA TGAGGTTCTC
    TGCAGATGGA TTTTAAGCGT GAAGAAAAAT TATCGGAAAA ATGTTGCTTA TCACAATTGG
    AGACATGCCT TTAATACAGC ACAGTGCATG TTTGCTGCTT TAAAATCTGG TAAAATTCAG
    AGCAAACTGA CTGACTTGGA AACGCTGGCT TTGCTGATAG CAACTCTAAG CCATGATTTG
    GATCACAGGG GAGTGAACAA CTCTTACATA CAAAGAAGTG AACATCCACT GGCTCAACTG
    TATTGCCACT CCATCATGGA GCATCACCAT TTTGATCAAT GCCTGATGAT CCTGAATAGT
    CCAGGAAACC AGATTCTCAG CAGCCTTTCA ATTGAAGAGT ACAAGGCAAC ACTGAAAATG
    ATAAAACAAG CCATCCTTGC AACAGACCTA GCACTATACA TAAAGAGGAG AGGAGAATTT
    TTTGAACTTC TAAGGAAAAA GCAGTTTAAT TGGGAAGATC CTATGCAGAA GGAGCTATTT
    TTAGCAATGC TGATGACTGC TTGTGATTTA TCAGCTATAA CCAAACCGTG GCCAGTTCAG
    CAACGGATTG CTGAGCTTGT AGCTACTGAG TTCTTTGATC AGGGAGATAA AGAGCGGAAG
    GAACTCAACA TAGAACCAAC AGATCTAATG AACAGAGAGA AGAAAAACAA AATTCCAAGC
    ATGCAGGTTG GATTCATTGA TGCTGTTTGC TTGCAGCTGT ATGAGGCCCT AACACATGTA
    TCAGAGGCCT GCTACCCTTT ACTGGATGGC TGTAGAAAAA ACAGGCAGAA ATGGCAGTCC
    TTAGCTGAAC AACAAGAGAA GAATCTGATT AATGGAGAAA GCAATCAAGC CAAACGGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35174
    Clone ID Related Accession (Same CDS sequence) XM_025149655.1 , XM_015276368.1 , XM_015276368.2
    Accession Version XM_015276368.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTTAATG CATGGTTTGC TGAGAGAGTT CATACCATCC CTGTCTGCAA GGAGGGGACC 
    AAGTCTCAGA GTGAATCCTG TGCTTGTCAG CAGCTTGCCT GCTCTGAGAA CACCAACTCT
    GGAACACCAG CAAGGAAAAT CTCTGCTTCA GAATTTGACA GACCCTTAAG GCCGATTTTT
    GTCAAGGACT CAGTAGGAGC AGTAAGCTTC CTCTCTGGCT CTGAAAAGAA GGAACAGATG
    CCTCTGCAAT CTCCAAGGAC TGGGACCAGT GCAGGGGATC GGTGCTCAAG GCTTTTAGAA
    CTTGTGAAAG ACATTTCTAG TCATTTGGAT GTCACAGCAC TTTGCCATAA AATTTTCCTG
    CATATCCATG AGCTTATAGC TGCTGATCGC TATTCCCTCT TCTTGGTCTG TGAGGATAGC
    TCTAACGAGA AGTTTCTTGT AAGCAGGCTG TTTGATGTGG CTGAAGGTTC CACCCTAGAA
    GAAGCTTCAA ATAACTGCAT TCGCCTGGAG TGGAACAAGG GCATCGTGGG CCACGTAGCT
    GCTATAGGCC AGCCTCTGAA CATCAAGAAT GCGTATGAGG ATCCAAGATT CAATGCAGAA
    GTTGACCAGA TCACAGGGTA TAAGACTCAG AGCATTCTCT GTATGCCAAT AAAGAATCAT
    AGGGAAGAGG TTGTTGGTGT GGCTCAAGCA ATTAATAAGA AATCTGGAAC TGGTGGGACT
    TTCACGGAAC AAGATGAAAA GGATTTTGCT GCATATTTGG CATTTTGTGG AATTGTTCTT
    CACAACGCTC AATTGTATGA AACATCTTTG CTTGAAAACA GGCGTAATCA GGTGCTTCTT
    GATCTTGCCA GCCTTATTTT TGAAGAACAA CAGTCTTTGG AAGTAATTCT GAAGAAGATA
    GCTGCCACTA TAATATCCTT CATGCAAGTG CAGAGGTGTA CCATCTTCAT AGTGGACGAA
    GATTGTACTG ATTCATTTTC TAGTGTTTTT CATATGGAAG CTGAGGAATT AGAAGATTCA
    GCAGAAAACC AGAAGAGGGA CTGTGATACA AACAAAATCA ATTATATGTA TGCTCAGTAT
    GTCAAGAATA CAATGGAGCC TCTCAACATC CCAGATGTCT GCAAAGACAG AAGATTTCCC
    TGGACAACTG ACAGTGCAGA AAGTGTAAGT CAACATATAA AGAGTTTGCT GTGTACACCA
    ATAAAAAATG GGAAAAAGAA TAAGGTGATA GGGGTTTGCC AGCTTGTGAA TAAGATGGAA
    GAAAACTCAG GCAAGATCAA GGCTTTCAAC AGAAATGATG AGCAGTTTCT GGAAGCATTT
    GTCATCTTTT GTGGCTTGGG GATCCAGAAT ACACAGATGT ATGAAGCTGT AGAAAGAGCA
    ATGGCCAAAC AGATGGTGAC ATTAGAGGTT CTCTCATACC ATGCTTCTGC TGCTGAGGAG
    GAATCAAGGG AGCTGCAGGT AACAGCGGCT GCTGTAGTAC CATCTGCACA ATCTCTCAAC
    CTAACTGACT TCAACTTCAG TGATTTTGAG CTATCAGATT TTGAAACAAC ACTGTGTACA
    ATTCGAATGT TCACAGACCT CAACCTGGTG CAGAATTTCC AAATGAAACA TGAGGTTCTC
    TGCAGATGGA TTTTAAGCGT GAAGAAAAAT TATCGGAAAA ATGTTGCTTA TCACAATTGG
    AGACATGCCT TTAATACAGC ACAGTGCATG TTTGCTGCTT TAAAATCTGG TAAAATTCAG
    AGCAAACTGA CTGACTTGGA AACGCTGGCT TTGCTGATAG CAACTCTAAG CCATGATTTG
    GATCACAGGG GAGTGAACAA CTCTTACATA CAAAGAAGTG AACATCCACT GGCTCAACTG
    TATTGCCACT CCATCATGGA GCATCACCAT TTTGATCAAT GCCTGATGAT CCTGAATAGT
    CCAGGAAACC AGATTCTCAG CAGCCTTTCA ATTGAAGAGT ACAAGGCAAC ACTGAAAATG
    ATAAAACAAG CCATCCTTGC AACAGACCTA GCACTATACA TAAAGAGGAG AGGAGAATTT
    TTTGAACTTC TAAGGAAAAA GCAGTTTAAT TGGGAAGATC CTATGCAGAA GGAGCTATTT
    TTAGCAATGC TGATGACTGC TTGTGATTTA TCAGCTATAA CCAAACCGTG GCCAGTTCAG
    CAACGGATTG CTGAGCTTGT AGCTACTGAG TTCTTTGATC AGGGAGATAA AGAGCGGAAG
    GAACTCAACA TAGAACCAAC AGATCTAATG AACAGAGAGA AGAAAAACAA AATTCCAAGC
    ATGCAGGTTG GATTCATTGA TGCTGTTTGC TTGCAGCTGT ATGAGGCCCT AACACATGTA
    TCAGAGGCCT GCTACCCTTT ACTGGATGGC TGTAGAAAAA ACAGGCAGAA ATGGCAGTCC
    TTAGCTGAAC AACAAGAGAA GAATCTGATT AATGGAGAAA GCAATCAAGC CAAACGGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131854.1
    CDS161..2623
    Translation

    Target ORF information:

    RefSeq Version XM_015276368.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276368.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTTAATG CATGGTTTGC TGAGAGAGTT CATACCATCC CTGTCTGCAA GGAGGGGACC 
    AAGTCTCAGA GTGAATCCTG TGCTTGTCAG CAGCTTGCCT GCTCTGAGAA CACCAACTCT
    GGAACACCAG CAAGGAAAAT CTCTGCTTCA GAATTTGACA GACCCTTAAG GCCGATTTTT
    GTCAAGGACT CAGTAGGAGC AGTAAGCTTC CTCTCTGGCT CTGAAAAGAA GGAACAGATG
    CCTCTGCAAT CTCCAAGGAC TGGGACCAGT GCAGGGGATC GGTGCTCAAG GCTTTTAGAA
    CTTGTGAAAG ACATTTCTAG TCATTTGGAT GTCACAGCAC TTTGCCATAA AATTTTCCTG
    CATATCCATG AGCTTATAGC TGCTGATCGC TATTCCCTCT TCTTGGTCTG TGAGGATAGC
    TCTAACGAGA AGTTTCTTGT AAGCAGGCTG TTTGATGTGG CTGAAGGTTC CACCCTAGAA
    GAAGCTTCAA ATAACTGCAT TCGCCTGGAG TGGAACAAGG GCATCGTGGG CCACGTAGCT
    GCTATAGGCC AGCCTCTGAA CATCAAGAAT GCGTATGAGG ATCCAAGATT CAATGCAGAA
    GTTGACCAGA TCACAGGGTA TAAGACTCAG AGCATTCTCT GTATGCCAAT AAAGAATCAT
    AGGGAAGAGG TTGTTGGTGT GGCTCAAGCA ATTAATAAGA AATCTGGAAC TGGTGGGACT
    TTCACGGAAC AAGATGAAAA GGATTTTGCT GCATATTTGG CATTTTGTGG AATTGTTCTT
    CACAACGCTC AATTGTATGA AACATCTTTG CTTGAAAACA GGCGTAATCA GGTGCTTCTT
    GATCTTGCCA GCCTTATTTT TGAAGAACAA CAGTCTTTGG AAGTAATTCT GAAGAAGATA
    GCTGCCACTA TAATATCCTT CATGCAAGTG CAGAGGTGTA CCATCTTCAT AGTGGACGAA
    GATTGTACTG ATTCATTTTC TAGTGTTTTT CATATGGAAG CTGAGGAATT AGAAGATTCA
    GCAGAAAACC AGAAGAGGGA CTGTGATACA AACAAAATCA ATTATATGTA TGCTCAGTAT
    GTCAAGAATA CAATGGAGCC TCTCAACATC CCAGATGTCT GCAAAGACAG AAGATTTCCC
    TGGACAACTG ACAGTGCAGA AAGTGTAAGT CAACATATAA AGAGTTTGCT GTGTACACCA
    ATAAAAAATG GGAAAAAGAA TAAGGTGATA GGGGTTTGCC AGCTTGTGAA TAAGATGGAA
    GAAAACTCAG GCAAGATCAA GGCTTTCAAC AGAAATGATG AGCAGTTTCT GGAAGCATTT
    GTCATCTTTT GTGGCTTGGG GATCCAGAAT ACACAGATGT ATGAAGCTGT AGAAAGAGCA
    ATGGCCAAAC AGATGGTGAC ATTAGAGGTT CTCTCATACC ATGCTTCTGC TGCTGAGGAG
    GAATCAAGGG AGCTGCAGGT AACAGCGGCT GCTGTAGTAC CATCTGCACA ATCTCTCAAC
    CTAACTGACT TCAACTTCAG TGATTTTGAG CTATCAGATT TTGAAACAAC ACTGTGTACA
    ATTCGAATGT TCACAGACCT CAACCTGGTG CAGAATTTCC AAATGAAACA TGAGGTTCTC
    TGCAGATGGA TTTTAAGCGT GAAGAAAAAT TATCGGAAAA ATGTTGCTTA TCACAATTGG
    AGACATGCCT TTAATACAGC ACAGTGCATG TTTGCTGCTT TAAAATCTGG TAAAATTCAG
    AGCAAACTGA CTGACTTGGA AACGCTGGCT TTGCTGATAG CAACTCTAAG CCATGATTTG
    GATCACAGGG GAGTGAACAA CTCTTACATA CAAAGAAGTG AACATCCACT GGCTCAACTG
    TATTGCCACT CCATCATGGA GCATCACCAT TTTGATCAAT GCCTGATGAT CCTGAATAGT
    CCAGGAAACC AGATTCTCAG CAGCCTTTCA ATTGAAGAGT ACAAGGCAAC ACTGAAAATG
    ATAAAACAAG CCATCCTTGC AACAGACCTA GCACTATACA TAAAGAGGAG AGGAGAATTT
    TTTGAACTTC TAAGGAAAAA GCAGTTTAAT TGGGAAGATC CTATGCAGAA GGAGCTATTT
    TTAGCAATGC TGATGACTGC TTGTGATTTA TCAGCTATAA CCAAACCGTG GCCAGTTCAG
    CAACGGATTG CTGAGCTTGT AGCTACTGAG TTCTTTGATC AGGGAGATAA AGAGCGGAAG
    GAACTCAACA TAGAACCAAC AGATCTAATG AACAGAGAGA AGAAAAACAA AATTCCAAGC
    ATGCAGGTTG GATTCATTGA TGCTGTTTGC TTGCAGCTGT ATGAGGCCCT AACACATGTA
    TCAGAGGCCT GCTACCCTTT ACTGGATGGC TGTAGAAAAA ACAGGCAGAA ATGGCAGTCC
    TTAGCTGAAC AACAAGAGAA GAATCTGATT AATGGAGAAA GCAATCAAGC CAAACGGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35174
    Clone ID Related Accession (Same CDS sequence) XM_025149655.1 , XM_015276368.1 , XM_015276368.2
    Accession Version XM_015276368.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276368.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTTAATG CATGGTTTGC TGAGAGAGTT CATACCATCC CTGTCTGCAA GGAGGGGACC 
    AAGTCTCAGA GTGAATCCTG TGCTTGTCAG CAGCTTGCCT GCTCTGAGAA CACCAACTCT
    GGAACACCAG CAAGGAAAAT CTCTGCTTCA GAATTTGACA GACCCTTAAG GCCGATTTTT
    GTCAAGGACT CAGTAGGAGC AGTAAGCTTC CTCTCTGGCT CTGAAAAGAA GGAACAGATG
    CCTCTGCAAT CTCCAAGGAC TGGGACCAGT GCAGGGGATC GGTGCTCAAG GCTTTTAGAA
    CTTGTGAAAG ACATTTCTAG TCATTTGGAT GTCACAGCAC TTTGCCATAA AATTTTCCTG
    CATATCCATG AGCTTATAGC TGCTGATCGC TATTCCCTCT TCTTGGTCTG TGAGGATAGC
    TCTAACGAGA AGTTTCTTGT AAGCAGGCTG TTTGATGTGG CTGAAGGTTC CACCCTAGAA
    GAAGCTTCAA ATAACTGCAT TCGCCTGGAG TGGAACAAGG GCATCGTGGG CCACGTAGCT
    GCTATAGGCC AGCCTCTGAA CATCAAGAAT GCGTATGAGG ATCCAAGATT CAATGCAGAA
    GTTGACCAGA TCACAGGGTA TAAGACTCAG AGCATTCTCT GTATGCCAAT AAAGAATCAT
    AGGGAAGAGG TTGTTGGTGT GGCTCAAGCA ATTAATAAGA AATCTGGAAC TGGTGGGACT
    TTCACGGAAC AAGATGAAAA GGATTTTGCT GCATATTTGG CATTTTGTGG AATTGTTCTT
    CACAACGCTC AATTGTATGA AACATCTTTG CTTGAAAACA GGCGTAATCA GGTGCTTCTT
    GATCTTGCCA GCCTTATTTT TGAAGAACAA CAGTCTTTGG AAGTAATTCT GAAGAAGATA
    GCTGCCACTA TAATATCCTT CATGCAAGTG CAGAGGTGTA CCATCTTCAT AGTGGACGAA
    GATTGTACTG ATTCATTTTC TAGTGTTTTT CATATGGAAG CTGAGGAATT AGAAGATTCA
    GCAGAAAACC AGAAGAGGGA CTGTGATACA AACAAAATCA ATTATATGTA TGCTCAGTAT
    GTCAAGAATA CAATGGAGCC TCTCAACATC CCAGATGTCT GCAAAGACAG AAGATTTCCC
    TGGACAACTG ACAGTGCAGA AAGTGTAAGT CAACATATAA AGAGTTTGCT GTGTACACCA
    ATAAAAAATG GGAAAAAGAA TAAGGTGATA GGGGTTTGCC AGCTTGTGAA TAAGATGGAA
    GAAAACTCAG GCAAGATCAA GGCTTTCAAC AGAAATGATG AGCAGTTTCT GGAAGCATTT
    GTCATCTTTT GTGGCTTGGG GATCCAGAAT ACACAGATGT ATGAAGCTGT AGAAAGAGCA
    ATGGCCAAAC AGATGGTGAC ATTAGAGGTT CTCTCATACC ATGCTTCTGC TGCTGAGGAG
    GAATCAAGGG AGCTGCAGGT AACAGCGGCT GCTGTAGTAC CATCTGCACA ATCTCTCAAC
    CTAACTGACT TCAACTTCAG TGATTTTGAG CTATCAGATT TTGAAACAAC ACTGTGTACA
    ATTCGAATGT TCACAGACCT CAACCTGGTG CAGAATTTCC AAATGAAACA TGAGGTTCTC
    TGCAGATGGA TTTTAAGCGT GAAGAAAAAT TATCGGAAAA ATGTTGCTTA TCACAATTGG
    AGACATGCCT TTAATACAGC ACAGTGCATG TTTGCTGCTT TAAAATCTGG TAAAATTCAG
    AGCAAACTGA CTGACTTGGA AACGCTGGCT TTGCTGATAG CAACTCTAAG CCATGATTTG
    GATCACAGGG GAGTGAACAA CTCTTACATA CAAAGAAGTG AACATCCACT GGCTCAACTG
    TATTGCCACT CCATCATGGA GCATCACCAT TTTGATCAAT GCCTGATGAT CCTGAATAGT
    CCAGGAAACC AGATTCTCAG CAGCCTTTCA ATTGAAGAGT ACAAGGCAAC ACTGAAAATG
    ATAAAACAAG CCATCCTTGC AACAGACCTA GCACTATACA TAAAGAGGAG AGGAGAATTT
    TTTGAACTTC TAAGGAAAAA GCAGTTTAAT TGGGAAGATC CTATGCAGAA GGAGCTATTT
    TTAGCAATGC TGATGACTGC TTGTGATTTA TCAGCTATAA CCAAACCGTG GCCAGTTCAG
    CAACGGATTG CTGAGCTTGT AGCTACTGAG TTCTTTGATC AGGGAGATAA AGAGCGGAAG
    GAACTCAACA TAGAACCAAC AGATCTAATG AACAGAGAGA AGAAAAACAA AATTCCAAGC
    ATGCAGGTTG GATTCATTGA TGCTGTTTGC TTGCAGCTGT ATGAGGCCCT AACACATGTA
    TCAGAGGCCT GCTACCCTTT ACTGGATGGC TGTAGAAAAA ACAGGCAGAA ATGGCAGTCC
    TTAGCTGAAC AACAAGAGAA GAATCTGATT AATGGAGAAA GCAATCAAGC CAAACGGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131854.1
    CDS159..2621
    Translation

    Target ORF information:

    RefSeq Version XM_015276368.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus phosphodiesterase 5A (PDE5A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276368.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTTAATG CATGGTTTGC TGAGAGAGTT CATACCATCC CTGTCTGCAA GGAGGGGACC 
    AAGTCTCAGA GTGAATCCTG TGCTTGTCAG CAGCTTGCCT GCTCTGAGAA CACCAACTCT
    GGAACACCAG CAAGGAAAAT CTCTGCTTCA GAATTTGACA GACCCTTAAG GCCGATTTTT
    GTCAAGGACT CAGTAGGAGC AGTAAGCTTC CTCTCTGGCT CTGAAAAGAA GGAACAGATG
    CCTCTGCAAT CTCCAAGGAC TGGGACCAGT GCAGGGGATC GGTGCTCAAG GCTTTTAGAA
    CTTGTGAAAG ACATTTCTAG TCATTTGGAT GTCACAGCAC TTTGCCATAA AATTTTCCTG
    CATATCCATG AGCTTATAGC TGCTGATCGC TATTCCCTCT TCTTGGTCTG TGAGGATAGC
    TCTAACGAGA AGTTTCTTGT AAGCAGGCTG TTTGATGTGG CTGAAGGTTC CACCCTAGAA
    GAAGCTTCAA ATAACTGCAT TCGCCTGGAG TGGAACAAGG GCATCGTGGG CCACGTAGCT
    GCTATAGGCC AGCCTCTGAA CATCAAGAAT GCGTATGAGG ATCCAAGATT CAATGCAGAA
    GTTGACCAGA TCACAGGGTA TAAGACTCAG AGCATTCTCT GTATGCCAAT AAAGAATCAT
    AGGGAAGAGG TTGTTGGTGT GGCTCAAGCA ATTAATAAGA AATCTGGAAC TGGTGGGACT
    TTCACGGAAC AAGATGAAAA GGATTTTGCT GCATATTTGG CATTTTGTGG AATTGTTCTT
    CACAACGCTC AATTGTATGA AACATCTTTG CTTGAAAACA GGCGTAATCA GGTGCTTCTT
    GATCTTGCCA GCCTTATTTT TGAAGAACAA CAGTCTTTGG AAGTAATTCT GAAGAAGATA
    GCTGCCACTA TAATATCCTT CATGCAAGTG CAGAGGTGTA CCATCTTCAT AGTGGACGAA
    GATTGTACTG ATTCATTTTC TAGTGTTTTT CATATGGAAG CTGAGGAATT AGAAGATTCA
    GCAGAAAACC AGAAGAGGGA CTGTGATACA AACAAAATCA ATTATATGTA TGCTCAGTAT
    GTCAAGAATA CAATGGAGCC TCTCAACATC CCAGATGTCT GCAAAGACAG AAGATTTCCC
    TGGACAACTG ACAGTGCAGA AAGTGTAAGT CAACATATAA AGAGTTTGCT GTGTACACCA
    ATAAAAAATG GGAAAAAGAA TAAGGTGATA GGGGTTTGCC AGCTTGTGAA TAAGATGGAA
    GAAAACTCAG GCAAGATCAA GGCTTTCAAC AGAAATGATG AGCAGTTTCT GGAAGCATTT
    GTCATCTTTT GTGGCTTGGG GATCCAGAAT ACACAGATGT ATGAAGCTGT AGAAAGAGCA
    ATGGCCAAAC AGATGGTGAC ATTAGAGGTT CTCTCATACC ATGCTTCTGC TGCTGAGGAG
    GAATCAAGGG AGCTGCAGGT AACAGCGGCT GCTGTAGTAC CATCTGCACA ATCTCTCAAC
    CTAACTGACT TCAACTTCAG TGATTTTGAG CTATCAGATT TTGAAACAAC ACTGTGTACA
    ATTCGAATGT TCACAGACCT CAACCTGGTG CAGAATTTCC AAATGAAACA TGAGGTTCTC
    TGCAGATGGA TTTTAAGCGT GAAGAAAAAT TATCGGAAAA ATGTTGCTTA TCACAATTGG
    AGACATGCCT TTAATACAGC ACAGTGCATG TTTGCTGCTT TAAAATCTGG TAAAATTCAG
    AGCAAACTGA CTGACTTGGA AACGCTGGCT TTGCTGATAG CAACTCTAAG CCATGATTTG
    GATCACAGGG GAGTGAACAA CTCTTACATA CAAAGAAGTG AACATCCACT GGCTCAACTG
    TATTGCCACT CCATCATGGA GCATCACCAT TTTGATCAAT GCCTGATGAT CCTGAATAGT
    CCAGGAAACC AGATTCTCAG CAGCCTTTCA ATTGAAGAGT ACAAGGCAAC ACTGAAAATG
    ATAAAACAAG CCATCCTTGC AACAGACCTA GCACTATACA TAAAGAGGAG AGGAGAATTT
    TTTGAACTTC TAAGGAAAAA GCAGTTTAAT TGGGAAGATC CTATGCAGAA GGAGCTATTT
    TTAGCAATGC TGATGACTGC TTGTGATTTA TCAGCTATAA CCAAACCGTG GCCAGTTCAG
    CAACGGATTG CTGAGCTTGT AGCTACTGAG TTCTTTGATC AGGGAGATAA AGAGCGGAAG
    GAACTCAACA TAGAACCAAC AGATCTAATG AACAGAGAGA AGAAAAACAA AATTCCAAGC
    ATGCAGGTTG GATTCATTGA TGCTGTTTGC TTGCAGCTGT ATGAGGCCCT AACACATGTA
    TCAGAGGCCT GCTACCCTTT ACTGGATGGC TGTAGAAAAA ACAGGCAGAA ATGGCAGTCC
    TTAGCTGAAC AACAAGAGAA GAATCTGATT AATGGAGAAA GCAATCAAGC CAAACGGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.