Aff1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aff1
Entrez Gene ID	17355
Full Name	AF4/FMR2 family, member 1
Synonyms	9630032B01Rik,AW319193,Af4,Mllt2h,Rob
General protein information	Preferred Names AF4/FMR2 family, member 1 Names AF4/FMR2 family member 1 homolog of human MLLT2 unidentified myeloid/lymphoid or mixed-lineage leukemia translocated to 2 homolog protein AF-4 proto-oncogene AF4 robotic
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 E5\|5 50.45 cM
Summary	This gene encodes a member of the AF4/ lymphoid nuclear protein related to the Fragile X E syndrome (FRAXE) family of proteins, which have been implicated in human childhood lymphoblastic leukemia, fragile chromosome X intellectual disability, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein domain, and a C-terminal homology domain. Knockout of the mouse gene by homologous recombination severely affects early events in lymphopoiesis, including precursor proliferation or recruitment, but is dispensable for terminal differentiation. In addition, an autosomal dominant missense mutation results in several phenotypes including ataxia and adult-onset Purkinje cell loss in the cerebellum, indicating a role in Purkinje cell maintenance and function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_133919.4	NP_598680.3	AF4/FMR2 family member 1 isoform 2
NM_001080798.2	NP_001074267.1	AF4/FMR2 family member 1 isoform 1
XM_006534801.3	XP_006534864.1	AF4/FMR2 family member 1 isoform X1
XM_006534801.4	XP_006534864.1	AF4/FMR2 family member 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AFF1	XP_001156948.2
Mus musculus (house mouse)	Aff1	NP_598680.3
Gallus gallus (chicken)	AFF1	XP_004941157.1
Homo sapiens (human)	AFF1	NP_001160165.1
	AFF1	XP_001095677.2
Canis lupus familiaris (dog)	AFF1	XP_005639216.1
Bos taurus (cattle)	AFF1	NP_001178454.1
Rattus norvegicus (Norway rat)	Aff1	NP_001100676.1

Gene Ontology
Bibliography

Related articles in PubMed

Amlexanox Downregulates S100A6 to Sensitize
Tamai H, Yamaguchi H, Miyake K, Takatori M, Kitano T, Yamanaka S, Yui S, Fukunaga K, Nakayama K, Inokuchi K
Cancer research77(16)4426-4433(2017 Aug)

Cooperative gene activation by AF4 and DOT1L drives MLL-rearranged leukemia.
Okuda H, Stanojevic B, Kanai A, Kawamura T, Takahashi S, Matsui H, Takaori-Kondo A, Yokoyama A
The Journal of clinical investigation127(5)1918-1931(2017 May)

Importance of a specific amino acid pairing for murine MLL leukemias driven by MLLT1/3 or AFF1/4.
Lokken AA, Achille NJ, Chang MJ, Lin JJ, Kuntimaddi A, Leach BI, Malik B, Nesbit JB, Zhang S, Bushweller JH, Zeleznik-Le NJ, Hemenway CS
Leukemia research38(11)1309-15(2014 Nov)

Mediation of Af4 protein function in the cerebellum by Siah proteins.
Oliver PL, Bitoun E, Clark J, Jones EL, Davies KE
Proceedings of the National Academy of Sciences of the United States of America101(41)14901-6(2004 Oct)

A mutation in Af4 is predicted to cause cerebellar ataxia and cataracts in the robotic mouse.
Isaacs AM, Oliver PL, Jones EL, Jeans A, Potter A, Hovik BH, Nolan PM, Vizor L, Glenister P, Simon AK, Gray IC, Spurr NK, Brown SD, Hunter AJ, Davies KE
The Journal of neuroscience : the official journal of the Society for Neuroscience23(5)1631-7(2003 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results reveal a cooperative transcriptional activation mechanism of AEP and DOT1L and suggest a molecular rationale for the simultaneous inhibition of the MLL fusion-AF4 complex and DOT1L for more effective treatment of MLL-rearranged leukemia.
Title: Cooperative gene activation by AF4 and DOT1L drives MLL-rearranged leukemia.

define a specific pairing of two amino acids that creates a salt bridge between MLLT1/3 and AFF proteins that is critically important for MLL-mediated transformation of HPCs
Title: Importance of a specific amino acid pairing for murine MLL leukemias driven by MLLT1/3 or AFF1/4.

Data show that the minimal 90-amino acid AF9 fragment in MLL-AF9 retains an ability to form higher order complexes with AF4*P-TEFb and with DOT1.
Title: Function of leukemogenic mixed lineage leukemia 1 (MLL) fusion proteins through distinct partner protein complexes.

small changes in the levels of a single transcriptional cofactor AF4 can deleteriously affect normal cerebellum function and opens new avenues of research for the manipulation of the IGF-1 pathway in the treatment of cerebellar ataxia in humans.
Title: AF4 is a critical regulator of the IGF-1 signaling pathway during Purkinje cell development.

enhancement of S100A6 expression plays an important role in MLL-AF4-associated leukemogenesis
Title: Multistep pathogenesis of leukemia via the MLL-AF4 chimeric gene/Flt3 gene tyrosine kinase domain (TKD) mutation-related enhancement of S100A6 expression.

The following Aff1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aff1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20813	NM_133919.4 Latest version!	Mus musculus AF4/FMR2 family, member 1 (Aff1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$713.30 $1019.00
OMu35385	XM_006534801.4 Latest version!	Mus musculus AF4/FMR2 family, member 1 (Aff1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu20814	NM_001080798.2 Latest version!	Mus musculus AF4/FMR2 family, member 1 (Aff1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$720.30 $1029.00
View Old Accession Versions >>
OMu35385	XM_006534801.3	Mus musculus AF4/FMR2 family, member 1 (Aff1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aff1 ( NM_133919.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598680.3 Mus musculus AF4/FMR2 family, member 1 (Aff1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu20813
Clone ID Related Accession (Same CDS sequence)	NM_133919.4
Accession Version	NM_133919.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	AF4/FMR2 family member 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK142554.1, BC131665.1, AK139900.1, EL607205.1, CF727488.1, DV649355.1, DV654133.1, AA139222.1, CV559177.1, CA528058.1, BQ554270.1 and AI836732.1. On Sep 17, 2015 this sequence version replaced NM_133919.3. Transcript Variant: This variant (2) uses an alternate 5'-terminal exon, which results in a different 5' UTR and use of an alternate start codon. It encodes isoform 2, which has a distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCCC ATTCCAGCTT GTACAATGAA GATAGAAACC TGCTTCGAAT CAGAGAGAAG 
GAAAGACGCA ACCAAGAAGC TCACCAGGAG AAGGAGGCAT TTCCCGAGAA GGCTCCCCTG 
TTTCCAGAGC CTTACAAGAC TGCAAAAGGC GATGAGCTAT CAAGTCGGAT CCAGACCATG 
CTGGGTGACT ATGAGGAGAT GAAGGAGTTC CTGAGCAGCA AGTCCCACCC CCACCGCCTG 
GATGGCTCTG AAGACAGGCC CGGGAAGCCC AGATATCCCT TAGGTCATGA CAGGGGGAAC 
GGGGCTGCAT CCAGCTCCCT CCGCACACAT GTCTACCACC AGCCTATCCA CACTTCTGCT 
CCCGGATCAC GTCCTGTCGG TAACATTAGC CACAGTCCAA AGATGGCACA GCCAAGGATG 
GAGCCAAGTC TCCACACCAA AATCTATGAT GGCCCACGTC TGACTCAAGA CCACCTCAGT 
CAGGGACATT GTTCCAGAAA GTGTGACCGA AGAGCTGAAG GAGACTCTGC TCCCGAGAGG 
AAGCTCTCGC CCTTGATCTC CTCTTTGCCA TCCCCGGTGC CCCCTCTATC ACCTGTACAT 
TCCAGGCTGC AGGGAACCAG CAAGGCTCAC AGCAGTGGCG TTAGCAGTAA AAGCTGCTGT 
GTGGCCAAGT CTTCCAAGGA CTTGGTGGCG AAGGCCCAAG ATAAAGAGAC TCCTCATGAC 
GGTTTGGTGG CAGTTACCAG CCTTGGATCA GCCCCTCCTC AGCCACCTTG CCAGACATTT 
CCACCCCCTC CTCTCCCCTC AAAAAGTGCT GCAATGCAGC AGAAGCCCAC GGCATATGTC 
CGTCCCATGG ACGGTCAGGA TCAGGCTCCC AGTGAGTCCC CCGAGCTGAA GCTGCCACTG 
GAGGACTATG GTCAGCAAAG CTTTGAGAAA CCAGACCTTA AAGTGCCTGC CAAAGCCAAG 
CTCACCAGAC TAAGGATGCC CTCTCAGTCG GTGGAGCAGC CGTACTCCAA TGAAGTCCAT 
TGCGTTGAAG AGATTCTGAA GGAAATGACC CACTCGTGGC CACCGCCTCT GACAGCCATA 
CACACACCCA GTACAGCCGA GCCGTCCAGA TTTCCTTTCC CCACAAAGGA CCCTCTGCAC 
GTCAGTCCTG CAACCCAGAG CCAAAAACAA TATGACACGC CTTCAAAAAC CCACCCCAAT 
CCCCAGCAAG GAACCTCCAT GCTGGAGGAC GACCTGCAGC TCAGCGACAG TGAAGACAGC 
GACACGGAGC AAGCCACAGA GAAGCCTCCA TCCCCACCTG CACCTCCCAG CGCTCCGCAG 
ACACTTCCCG AGCCCGTGGC CTCAGCACAT TCCAGTAGTG GGGAGTCAGA GAGCAGCGAG 
TCAGACAGTT CCTCAGACTC TGAGAGTGAG AGCAGCTCCA GCGACAGCGA GGAGGAGGAA 
GAGAACGAGC CCCTGGAGAC CCGGGCTCCC GAGCCCGAGC CTCCGACGAC AAACAAATGG 
CAGCTGGACA ACTGGTTGAC CAAAGTCAAC CAACCCTCAG TGCCCCTGGA TGGCCGGGGG 
AGCACAGAGT CTCCACAATG GCGCCAGGAA AGTAAGGGTG TCGCTGAGGG CAGCAGTGAC 
CAGCAGCATC CTGATTCCAA AGACCCTCTC CCAAAGAGCT CCAGCAAGAC TCTCCGGGGT 
CCCTCTGAGG GGCCCCAGCC TGGGAAGAGG GGCTGTCCGA AATCCCCCGC TCAACAGGAA 
CCCCCACCTC GGCAAACCGT GGGCAGCAAA CAACCCAGGA AACCCGCCAA GGGCTCCGGC 
CAGGCAGAGC CCCAGGCCAG CTCGCAGGCA GAGAGTGAAG TAGGACCCCT TCCCTACGGC 
TCAAAGGAGC AGACTTCCAA AGACAGACCC AAGGTGAAGA CGAAAGGCAG GCCGCGTGCC 
GTGGGCAGTC GGGAGCCTAA GCCAGAGGTC CCTGCGCCCA CGCCTCAGGC AGCCGTGCCC 
AGACCGAAGC CGCCTGTGCC CACCCCCAGT GAGAAGAGGA AGCACAAGAG CTCCACGGCC 
CCCTCCAAGG CGCCCTCAGC CCCCCAGCCT CCGAAGGACA GTGCTGGGGA CAGGAACCCA 
GAACACTCTG CTCTTGTCTC CTTGACTCAG AGCCAGGGTC CATCCCACAG CAGCAGGGGC 
AGCAGTGGCA GCGTCAGGAC TAGTGGCTGC CGACAGGCTG TGATTGCCCA AGGGGACGGC 
TGTAAAGACA AGCTCCTGTT GCCTTTGAGA GACACCAAAT TGCTCTCGCC GCTCAGGGAC 
TCCCCACCAC CGACAAGCTT GGTGGTGAAG ATAACCCTAG ACCTCCTCAC GAGGATTCCC 
CAGCCTCTGG GGAAGGGGAG CCGCCCCAGG AAAGCAGAAG ACAAGCAGCT GTCAGCAGGG 
AAGAAGCAGG ACTCGGAGAC CAAGAGCTGT GACAGCTCCA GCAGAGTGAC CAAGAAGAGA 
AAGGGTGACC CAGAAAAAGA GCACAGTAAC AAGAGACACA AACTGGATAA GTCGCAGACT 
GCGTCATCTT CATCTTCCCA TACAGAATCT TCCAGAACAA AAGCGCCCAG GTCCTCCTCA 
GAGAACTCAA GAAAGGAAAT GCTGCCTCCA GCATCGGCGT CCTCAGTCTC CTCTTCCTCA 
TCCTCCCAGA AGCCAAGCAG ACCTGCACAG AAGAGGCCAA GGCCAGACGA AGACACGTGT 
AGCCAGGAAC CCCCCAGAAG TGCCAGCAGC ACAAAGAGCA GCTCTACAGA CCCTCCCGCT 
CCCAAGCACA GAAAAGTACA GGCCAGGGGC TCTGAACACA AGGGATCTTC TGGGGATGCT 
GCGAATGCTG CAAATCCTTT TCCAGTGCCT TCTTTGCCGA ATGGTAACGC TAAACCAGGG 
AAGCCACAGG TGAAGTCTGA CAGACAACAA GCTGACTTTC ACATGAAGGA GGCTAAAAAG 
CTGAAGTGCA AAGCAGAGAC AATGGTAGAC AAGGCTGGAA AAGCCTTCAA GTATTTGGAA 
GCCGTCCTCT CTTTTATCGA GTGTGGAATG GCCTCAGAGT CAGAAAGCTC AGCCAAGTCG 
GCGTACGCTG TGTACTCAGA AACCATAGAC CTCATTAGGT ATGTGATGTC ACTAAAATGC 
TTCTCAGATA ACACGATGCC AGCACAAGAG AAGATATTTG CTGTTTTATG CTTGCGTTGC 
CAGTCCCTTT TGAACATGGC GATGTTTCGA TGTAAGAAAG ACACAGTGAT GAAGTATTCT 
CGTACTCTCA GTGAACACTT CAAGAGTACT TCCAAAGTGG CCCAGGCACC TTCTCCATGC 
ACGGCAAGAA GCACAGGCGT TCCGTCCCCC CTCTCCCCAA TGCCTTCTCC TGCCAGCTCC 
GTGGGGTCCC AATCAAGTGC TGGCAGCAGT ATGGGGAGCG TCGGGGTGAC GGCCACCGTC 
AGTACTCCAG TCTCCATCCA GAACATGACC TCCTCCTATG TCACCATCAC ATCCCATGTC 
CTTACGGCCT TTAGCCTTTG GGAACAGGCC GAGGCCCTCA CAAGGAAGAA CAAAGAATTC 
TTCGCTCAGC TCAGCACAAA AGTGCGTGTG TTGGCCCTCA ACAGCAGCCT GGTGGACCTG 
GTGCACTACA CAAGACAGGG CCTGCAGCGG CTAAAACAGT CACCCAAAGG CCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598680.3
CDS	168..3824
Translation	MAAHSSLYNEDRNLLRIREKERRNQEAHQEKEAFPEKAPLFPEPYKTAKGDELSSRIQTMLGDYEEMKEFLSSKSHPHRLDGSEDRPGKPRYPLGHDRGNGAASSSLRTHVYHQPIHTSAPGSRPVGNISHSPKMAQPRMEPSLHTKIYDGPRLTQDHLSQGHCSRKCDRRAEGDSAPERKLSPLISSLPSPVPPLSPVHSRLQGTSKAHSSGVSSKSCCVAKSSKDLVAKAQDKETPHDGLVAVTSLGSAPPQPPCQTFPPPPLPSKSAAMQQKPTAYVRPMDGQDQAPSESPELKLPLEDYGQQSFEKPDLKVPAKAKLTRLRMPSQSVEQPYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSRFPFPTKDPLHVSPATQSQKQYDTPSKTHPNPQQGTSMLEDDLQLSDSEDSDTEQATEKPPSPPAPPSAPQTLPEPVASAHSSSGESESSESDSSSDSESESSSSDSEEEEENEPLETRAPEPEPPTTNKWQLDNWLTKVNQPSVPLDGRGSTESPQWRQESKGVAEGSSDQQHPDSKDPLPKSSSKTLRGPSEGPQPGKRGCPKSPAQQEPPPRQTVGSKQPRKPAKGSGQAEPQASSQAESEVGPLPYGSKEQTSKDRPKVKTKGRPRAVGSREPKPEVPAPTPQAAVPRPKPPVPTPSEKRKHKSSTAPSKAPSAPQPPKDSAGDRNPEHSALVSLTQSQGPSHSSRGSSGSVRTSGCRQAVIAQGDGCKDKLLLPLRDTKLLSPLRDSPPPTSLVVKITLDLLTRIPQPLGKGSRPRKAEDKQLSAGKKQDSETKSCDSSSRVTKKRKGDPEKEHSNKRHKLDKSQTASSSSSHTESSRTKAPRSSSENSRKEMLPPASASSVSSSSSSQKPSRPAQKRPRPDEDTCSQEPPRSASSTKSSSTDPPAPKHRKVQARGSEHKGSSGDAANAANPFPVPSLPNGNAKPGKPQVKSDRQQADFHMKEAKKLKCKAETMVDKAGKAFKYLEAVLSFIECGMASESESSAKSAYAVYSETIDLIRYVMSLKCFSDNTMPAQEKIFAVLCLRCQSLLNMAMFRCKKDTVMKYSRTLSEHFKSTSKVAQAPSPCTARSTGVPSPLSPMPSPASSVGSQSSAGSSMGSVGVTATVSTPVSIQNMTSSYVTITSHVLTAFSLWEQAEALTRKNKEFFAQLSTKVRVLALNSSLVDLVHYTRQGLQRLKQSPKGP

Target ORF information:

RefSeq Version	NM_133919.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus AF4/FMR2 family, member 1 (Aff1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133919.4

ORF Insert Sequence:

ATGGCAGCCC ATTCCAGCTT GTACAATGAA GATAGAAACC TGCTTCGAAT CAGAGAGAAG 
GAAAGACGCA ACCAAGAAGC TCACCAGGAG AAGGAGGCAT TTCCCGAGAA GGCTCCCCTG 
TTTCCAGAGC CTTACAAGAC TGCAAAAGGC GATGAGCTAT CAAGTCGGAT CCAGACCATG 
CTGGGTGACT ATGAGGAGAT GAAGGAGTTC CTGAGCAGCA AGTCCCACCC CCACCGCCTG 
GATGGCTCTG AAGACAGGCC CGGGAAGCCC AGATATCCCT TAGGTCATGA CAGGGGGAAC 
GGGGCTGCAT CCAGCTCCCT CCGCACACAT GTCTACCACC AGCCTATCCA CACTTCTGCT 
CCCGGATCAC GTCCTGTCGG TAACATTAGC CACAGTCCAA AGATGGCACA GCCAAGGATG 
GAGCCAAGTC TCCACACCAA AATCTATGAT GGCCCACGTC TGACTCAAGA CCACCTCAGT 
CAGGGACATT GTTCCAGAAA GTGTGACCGA AGAGCTGAAG GAGACTCTGC TCCCGAGAGG 
AAGCTCTCGC CCTTGATCTC CTCTTTGCCA TCCCCGGTGC CCCCTCTATC ACCTGTACAT 
TCCAGGCTGC AGGGAACCAG CAAGGCTCAC AGCAGTGGCG TTAGCAGTAA AAGCTGCTGT 
GTGGCCAAGT CTTCCAAGGA CTTGGTGGCG AAGGCCCAAG ATAAAGAGAC TCCTCATGAC 
GGTTTGGTGG CAGTTACCAG CCTTGGATCA GCCCCTCCTC AGCCACCTTG CCAGACATTT 
CCACCCCCTC CTCTCCCCTC AAAAAGTGCT GCAATGCAGC AGAAGCCCAC GGCATATGTC 
CGTCCCATGG ACGGTCAGGA TCAGGCTCCC AGTGAGTCCC CCGAGCTGAA GCTGCCACTG 
GAGGACTATG GTCAGCAAAG CTTTGAGAAA CCAGACCTTA AAGTGCCTGC CAAAGCCAAG 
CTCACCAGAC TAAGGATGCC CTCTCAGTCG GTGGAGCAGC CGTACTCCAA TGAAGTCCAT 
TGCGTTGAAG AGATTCTGAA GGAAATGACC CACTCGTGGC CACCGCCTCT GACAGCCATA 
CACACACCCA GTACAGCCGA GCCGTCCAGA TTTCCTTTCC CCACAAAGGA CCCTCTGCAC 
GTCAGTCCTG CAACCCAGAG CCAAAAACAA TATGACACGC CTTCAAAAAC CCACCCCAAT 
CCCCAGCAAG GAACCTCCAT GCTGGAGGAC GACCTGCAGC TCAGCGACAG TGAAGACAGC 
GACACGGAGC AAGCCACAGA GAAGCCTCCA TCCCCACCTG CACCTCCCAG CGCTCCGCAG 
ACACTTCCCG AGCCCGTGGC CTCAGCACAT TCCAGTAGTG GGGAGTCAGA GAGCAGCGAG 
TCAGACAGTT CCTCAGACTC TGAGAGTGAG AGCAGCTCCA GCGACAGCGA GGAGGAGGAA 
GAGAACGAGC CCCTGGAGAC CCGGGCTCCC GAGCCCGAGC CTCCGACGAC AAACAAATGG 
CAGCTGGACA ACTGGTTGAC CAAAGTCAAC CAACCCTCAG TGCCCCTGGA TGGCCGGGGG 
AGCACAGAGT CTCCACAATG GCGCCAGGAA AGTAAGGGTG TCGCTGAGGG CAGCAGTGAC 
CAGCAGCATC CTGATTCCAA AGACCCTCTC CCAAAGAGCT CCAGCAAGAC TCTCCGGGGT 
CCCTCTGAGG GGCCCCAGCC TGGGAAGAGG GGCTGTCCGA AATCCCCCGC TCAACAGGAA 
CCCCCACCTC GGCAAACCGT GGGCAGCAAA CAACCCAGGA AACCCGCCAA GGGCTCCGGC 
CAGGCAGAGC CCCAGGCCAG CTCGCAGGCA GAGAGTGAAG TAGGACCCCT TCCCTACGGC 
TCAAAGGAGC AGACTTCCAA AGACAGACCC AAGGTGAAGA CGAAAGGCAG GCCGCGTGCC 
GTGGGCAGTC GGGAGCCTAA GCCAGAGGTC CCTGCGCCCA CGCCTCAGGC AGCCGTGCCC 
AGACCGAAGC CGCCTGTGCC CACCCCCAGT GAGAAGAGGA AGCACAAGAG CTCCACGGCC 
CCCTCCAAGG CGCCCTCAGC CCCCCAGCCT CCGAAGGACA GTGCTGGGGA CAGGAACCCA 
GAACACTCTG CTCTTGTCTC CTTGACTCAG AGCCAGGGTC CATCCCACAG CAGCAGGGGC 
AGCAGTGGCA GCGTCAGGAC TAGTGGCTGC CGACAGGCTG TGATTGCCCA AGGGGACGGC 
TGTAAAGACA AGCTCCTGTT GCCTTTGAGA GACACCAAAT TGCTCTCGCC GCTCAGGGAC 
TCCCCACCAC CGACAAGCTT GGTGGTGAAG ATAACCCTAG ACCTCCTCAC GAGGATTCCC 
CAGCCTCTGG GGAAGGGGAG CCGCCCCAGG AAAGCAGAAG ACAAGCAGCT GTCAGCAGGG 
AAGAAGCAGG ACTCGGAGAC CAAGAGCTGT GACAGCTCCA GCAGAGTGAC CAAGAAGAGA 
AAGGGTGACC CAGAAAAAGA GCACAGTAAC AAGAGACACA AACTGGATAA GTCGCAGACT 
GCGTCATCTT CATCTTCCCA TACAGAATCT TCCAGAACAA AAGCGCCCAG GTCCTCCTCA 
GAGAACTCAA GAAAGGAAAT GCTGCCTCCA GCATCGGCGT CCTCAGTCTC CTCTTCCTCA 
TCCTCCCAGA AGCCAAGCAG ACCTGCACAG AAGAGGCCAA GGCCAGACGA AGACACGTGT 
AGCCAGGAAC CCCCCAGAAG TGCCAGCAGC ACAAAGAGCA GCTCTACAGA CCCTCCCGCT 
CCCAAGCACA GAAAAGTACA GGCCAGGGGC TCTGAACACA AGGGATCTTC TGGGGATGCT 
GCGAATGCTG CAAATCCTTT TCCAGTGCCT TCTTTGCCGA ATGGTAACGC TAAACCAGGG 
AAGCCACAGG TGAAGTCTGA CAGACAACAA GCTGACTTTC ACATGAAGGA GGCTAAAAAG 
CTGAAGTGCA AAGCAGAGAC AATGGTAGAC AAGGCTGGAA AAGCCTTCAA GTATTTGGAA 
GCCGTCCTCT CTTTTATCGA GTGTGGAATG GCCTCAGAGT CAGAAAGCTC AGCCAAGTCG 
GCGTACGCTG TGTACTCAGA AACCATAGAC CTCATTAGGT ATGTGATGTC ACTAAAATGC 
TTCTCAGATA ACACGATGCC AGCACAAGAG AAGATATTTG CTGTTTTATG CTTGCGTTGC 
CAGTCCCTTT TGAACATGGC GATGTTTCGA TGTAAGAAAG ACACAGTGAT GAAGTATTCT 
CGTACTCTCA GTGAACACTT CAAGAGTACT TCCAAAGTGG CCCAGGCACC TTCTCCATGC 
ACGGCAAGAA GCACAGGCGT TCCGTCCCCC CTCTCCCCAA TGCCTTCTCC TGCCAGCTCC 
GTGGGGTCCC AATCAAGTGC TGGCAGCAGT ATGGGGAGCG TCGGGGTGAC GGCCACCGTC 
AGTACTCCAG TCTCCATCCA GAACATGACC TCCTCCTATG TCACCATCAC ATCCCATGTC 
CTTACGGCCT TTAGCCTTTG GGAACAGGCC GAGGCCCTCA CAAGGAAGAA CAAAGAATTC 
TTCGCTCAGC TCAGCACAAA AGTGCGTGTG TTGGCCCTCA ACAGCAGCCT GGTGGACCTG 
GTGCACTACA CAAGACAGGG CCTGCAGCGG CTAAAACAGT CACCCAAAGG CCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aff1 ( XM_006534801.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534864.1 Mus musculus AF4/FMR2 family, member 1 (Aff1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35385
Clone ID Related Accession (Same CDS sequence)	XM_006534801.3 , XM_006534801.4
Accession Version	XM_006534801.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	AF4/FMR2 family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006534801.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTTA AGGACAGCAG CAGCAGCAGC TTGTACAATG AAGATAGAAA CCTGCTTCGA 
ATCAGAGAGA AGGAAAGACG CAACCAAGAA GCTCACCAGG AGAAGGAGGC ATTTCCCGAG 
AAGGCTCCCC TGTTTCCAGA GCCTTACAAG ACTGCAAAAG GCGATGAGCT ATCAAGTCGG 
ATCCAGACCA TGCTGGGTGA CTATGAGGAG ATGAAGGAGT TCCTGAGCAG CAAGTCCCAC 
CCCCACCGCC TGGATGGCTC TGAAGACAGG CCCGGGAAGC CCAGATATCC CTTAGGTCAT 
GACAGGGGGA ACGGGGCTGC ATCCAGCTCC CTCCGCACAC ATGTCTACCA CCAGCCTATC 
CACACTTCTG CTCCCGGATC ACGTCCTGTC GGTAACATTA GCCACAGTCC AAAGATGGCA 
CAGCCAAGGA TGGAGCCAAG TCTCCACACC AAAATCTATG ATGGCCCACG TCTGACTCAA 
GACCACCTCA GTCAGGGACA TTGTTCCAGA AAGTGTGACC GAAGAGCTGA AGGAGACTCT 
GCTCCCGAGA GGAAGCTCTC GCCCTTGATC TCCTCTTTGC CATCCCCGGT GCCCCCTCTA 
TCACCTGTAC ATTCCAGGCT GCAGGGAACC AGCAAGGCTC ACAGCAGTGG CGTTAGCAGT 
AAAAGCTGCT GTGTGGCCAA GTCTTCCAAG GACTTGGTGG CGAAGGCCCA AGATAAAGAG 
ACTCCTCATG ACGGTTTGGT GGCAGTTACC AGCCTTGGAT CAGCCCCTCC TCAGCCACCT 
TGCCAGACAT TTCCACCCCC TCCTCTCCCC TCAAAAAGTG CTGCAATGCA GCAGAAGCCC 
ACGGCATATG TCCGTCCCAT GGACGGTCAG GATCAGGCTC CCAGTGAGTC CCCCGAGCTG 
AAGCTGCCAC TGGAGGACTA TGGTCAGCAA AGCTTTGAGA AACCAGACCT TAAAGTGCCT 
GCCAAAGCCA AGCTCACCAG ACTAAGGATG CCCTCTCAGT CGGTGGAGCA GCCGTACTCC 
AATGAAGTCC ATTGCGTTGA AGAGATTCTG AAGGAAATGA CCCACTCGTG GCCACCGCCT 
CTGACAGCCA TACACACACC CAGTACAGCC GAGCCGTCCA GATTTCCTTT CCCCACAAAG 
GACCCTCTGC ACGTCAGTCC TGCAACCCAG AGCCAAAAAC AATATGACAC GCCTTCAAAA 
ACCCACCCCA ATCCCCAGCA AGGAACCTCC ATGCTGGAGG ACGACCTGCA GCTCAGCGAC 
AGTGAAGACA GCGACACGGA GCAAGCCACA GAGAAGCCTC CATCCCCACC TGCACCTCCC 
AGCGCTCCGC AGACACTTCC CGAGCCCGTG GCCTCAGCAC ATTCCAGTAG TGGGGAGTCA 
GAGAGCAGCG AGTCAGACAG TTCCTCAGAC TCTGAGAGTG AGAGCAGCTC CAGCGACAGC 
GAGGAGGAGG AAGAGAACGA GCCCCTGGAG ACCCGGGCTC CCGAGCCCGA GCCTCCGACG 
ACAAACAAAT GGCAGCTGGA CAACTGGTTG ACCAAAGTCA ACCAACCCTC AGTGCCCCTG 
GATGGCCGGG GGAGCACAGA GTCTCCACAA TGGCGCCAGG AAAGTAAGGG TGTCGCTGAG 
GGCAGCAGTG ACCAGCAGCA TCCTGATTCC AAAGACCCTC TCCCAAAGAG CTCCAGCAAG 
ACTCTCCGGG GTCCCTCTGA GGGGCCCCAG CCTGGGAAGA GGGGCTGTCC GAAATCCCCC 
GCTCAACAGG AACCCCCACC TCGGCAAACC GTGGGCAGCA AACAACCCAG GAAACCCGCC 
AAGGGCTCCG GCCAGGCAGA GCCCCAGGCC AGCTCGCAGG CAGAGAGTGA AGTAGGACCC 
CTTCCCTACG GCTCAAAGGA GCAGACTTCC AAAGACAGAC CCAAGGTGAA GACGAAAGGC 
AGGCCGCGTG CCGTGGGCAG TCGGGAGCCT AAGCCAGAGG TCCCTGCGCC CACGCCTCAG 
GCAGCCGTGC CCAGACCGAA GCCGCCTGTG CCCACCCCCA GTGAGAAGAG GAAGCACAAG 
AGCTCCACGG CCCCCTCCAA GGCGCCCTCA GCCCCCCAGC CTCCGAAGGA CAGTGCTGGG 
GACAGGAACC CAGAACACTC TGCTCTTGTC TCCTTGACTC AGAGCCAGGG TCCATCCCAC 
AGCAGCAGGG GCAGCAGTGG CAGCGTCAGG ACTAGTGGCT GCCGACAGGC TGTGATTGCC 
CAAGGGGACG GCTGTAAAGA CAAGCTCCTG TTGCCTTTGA GAGACACCAA ATTGCTCTCG 
CCGCTCAGGG ACTCCCCACC ACCGACAAGC TTGGTGGTGA AGATAACCCT AGACCTCCTC 
ACGAGGATTC CCCAGCCTCT GGGGAAGGGG AGCCGCCCCA GGAAAGCAGA AGACAAGCAG 
CTGTCAGCAG GGAAGAAGCA GGACTCGGAG ACCAAGAGCT GTGACAGCTC CAGCAGAGTG 
ACCAAGAAGA GAAAGGGTGA CCCAGAAAAA GAGCACAGTA ACAAGAGACA CAAACTGGAT 
AAGTCGCAGA CTGCGTCATC TTCATCTTCC CATACAGAAT CTTCCAGAAC AAAAGCGCCC 
AGGTCCTCCT CAGAGAACTC AAGAAAGGAA ATGCTGCCTC CAGCATCGGC GTCCTCAGTC 
TCCTCTTCCT CATCCTCCCA GAAGCCAAGC AGACCTGCAC AGAAGAGGCC AAGGCCAGAC 
GAAGACACGT GTAGCCAGGA ACCCCCCAGA AGTGCCAGCA GCACAAAGAG CAGCTCTACA 
GACCCTCCCG CTCCCAAGCA CAGAAAAGTA CAGGCCAGGG GCTCTGAACA CAAGGGATCT 
TCTGGGGATG CTGCGAATGC TGCAAATCCT TTTCCAGTGC CTTCTTTGCC GAATGGTAAC 
GCTAAACCAG GGAAGCCACA GGTGAAGTCT GACAGACAAC AAGCTGACTT TCACATGAAG 
GAGGCTAAAA AGCTGAAGTG CAAAGCAGAG ACAATGGTAG ACAAGGCTGG AAAAGCCTTC 
AAGTATTTGG AAGCCGTCCT CTCTTTTATC GAGTGTGGAA TGGCCTCAGA GTCAGAAAGC 
TCAGCCAAGT CGGCGTACGC TGTGTACTCA GAAACCATAG ACCTCATTAG GTATGTGATG 
TCACTAAAAT GCTTCTCAGA TAACACGATG CCAGCACAAG AGAAGATATT TGCTGTTTTA 
TGCTTGCGTT GCCAGTCCCT TTTGAACATG GCGATGTTTC GATGTAAGAA AGACACAGTG 
ATGAAGTATT CTCGTACTCT CAGTGAACAC TTCAAGAGTA CTTCCAAAGT GGCCCAGGCA 
CCTTCTCCAT GCACGGCAAG AAGCACAGGC GTTCCGTCCC CCCTCTCCCC AATGCCTTCT 
CCTGCCAGCT CCGTGGGGTC CCAATCAAGT GCTGGCAGCA GTATGGGGAG CGTCGGGGTG 
ACGGCCACCG TCAGTACTCC AGTCTCCATC CAGAACATGA CCTCCTCCTA TGTCACCATC 
ACATCCCATG TCCTTACGGC CTTTAGCCTT TGGGAACAGG CCGAGGCCCT CACAAGGAAG 
AACAAAGAAT TCTTCGCTCA GCTCAGCACA AAAGTGCGTG TGTTGGCCCT CAACAGCAGC 
CTGGTGGACC TGGTGCACTA CACAAGACAG GGCCTGCAGC GGCTAAAACA GTCACCCAAA 
GGCCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534864.1
CDS	270..3938
Translation	MAFKDSSSSSLYNEDRNLLRIREKERRNQEAHQEKEAFPEKAPLFPEPYKTAKGDELSSRIQTMLGDYEEMKEFLSSKSHPHRLDGSEDRPGKPRYPLGHDRGNGAASSSLRTHVYHQPIHTSAPGSRPVGNISHSPKMAQPRMEPSLHTKIYDGPRLTQDHLSQGHCSRKCDRRAEGDSAPERKLSPLISSLPSPVPPLSPVHSRLQGTSKAHSSGVSSKSCCVAKSSKDLVAKAQDKETPHDGLVAVTSLGSAPPQPPCQTFPPPPLPSKSAAMQQKPTAYVRPMDGQDQAPSESPELKLPLEDYGQQSFEKPDLKVPAKAKLTRLRMPSQSVEQPYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSRFPFPTKDPLHVSPATQSQKQYDTPSKTHPNPQQGTSMLEDDLQLSDSEDSDTEQATEKPPSPPAPPSAPQTLPEPVASAHSSSGESESSESDSSSDSESESSSSDSEEEEENEPLETRAPEPEPPTTNKWQLDNWLTKVNQPSVPLDGRGSTESPQWRQESKGVAEGSSDQQHPDSKDPLPKSSSKTLRGPSEGPQPGKRGCPKSPAQQEPPPRQTVGSKQPRKPAKGSGQAEPQASSQAESEVGPLPYGSKEQTSKDRPKVKTKGRPRAVGSREPKPEVPAPTPQAAVPRPKPPVPTPSEKRKHKSSTAPSKAPSAPQPPKDSAGDRNPEHSALVSLTQSQGPSHSSRGSSGSVRTSGCRQAVIAQGDGCKDKLLLPLRDTKLLSPLRDSPPPTSLVVKITLDLLTRIPQPLGKGSRPRKAEDKQLSAGKKQDSETKSCDSSSRVTKKRKGDPEKEHSNKRHKLDKSQTASSSSSHTESSRTKAPRSSSENSRKEMLPPASASSVSSSSSSQKPSRPAQKRPRPDEDTCSQEPPRSASSTKSSSTDPPAPKHRKVQARGSEHKGSSGDAANAANPFPVPSLPNGNAKPGKPQVKSDRQQADFHMKEAKKLKCKAETMVDKAGKAFKYLEAVLSFIECGMASESESSAKSAYAVYSETIDLIRYVMSLKCFSDNTMPAQEKIFAVLCLRCQSLLNMAMFRCKKDTVMKYSRTLSEHFKSTSKVAQAPSPCTARSTGVPSPLSPMPSPASSVGSQSSAGSSMGSVGVTATVSTPVSIQNMTSSYVTITSHVLTAFSLWEQAEALTRKNKEFFAQLSTKVRVLALNSSLVDLVHYTRQGLQRLKQSPKGP

Target ORF information:

RefSeq Version	XM_006534801.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus AF4/FMR2 family, member 1 (Aff1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534801.4

ORF Insert Sequence:

ATGGCTTTTA AGGACAGCAG CAGCAGCAGC TTGTACAATG AAGATAGAAA CCTGCTTCGA 
ATCAGAGAGA AGGAAAGACG CAACCAAGAA GCTCACCAGG AGAAGGAGGC ATTTCCCGAG 
AAGGCTCCCC TGTTTCCAGA GCCTTACAAG ACTGCAAAAG GCGATGAGCT ATCAAGTCGG 
ATCCAGACCA TGCTGGGTGA CTATGAGGAG ATGAAGGAGT TCCTGAGCAG CAAGTCCCAC 
CCCCACCGCC TGGATGGCTC TGAAGACAGG CCCGGGAAGC CCAGATATCC CTTAGGTCAT 
GACAGGGGGA ACGGGGCTGC ATCCAGCTCC CTCCGCACAC ATGTCTACCA CCAGCCTATC 
CACACTTCTG CTCCCGGATC ACGTCCTGTC GGTAACATTA GCCACAGTCC AAAGATGGCA 
CAGCCAAGGA TGGAGCCAAG TCTCCACACC AAAATCTATG ATGGCCCACG TCTGACTCAA 
GACCACCTCA GTCAGGGACA TTGTTCCAGA AAGTGTGACC GAAGAGCTGA AGGAGACTCT 
GCTCCCGAGA GGAAGCTCTC GCCCTTGATC TCCTCTTTGC CATCCCCGGT GCCCCCTCTA 
TCACCTGTAC ATTCCAGGCT GCAGGGAACC AGCAAGGCTC ACAGCAGTGG CGTTAGCAGT 
AAAAGCTGCT GTGTGGCCAA GTCTTCCAAG GACTTGGTGG CGAAGGCCCA AGATAAAGAG 
ACTCCTCATG ACGGTTTGGT GGCAGTTACC AGCCTTGGAT CAGCCCCTCC TCAGCCACCT 
TGCCAGACAT TTCCACCCCC TCCTCTCCCC TCAAAAAGTG CTGCAATGCA GCAGAAGCCC 
ACGGCATATG TCCGTCCCAT GGACGGTCAG GATCAGGCTC CCAGTGAGTC CCCCGAGCTG 
AAGCTGCCAC TGGAGGACTA TGGTCAGCAA AGCTTTGAGA AACCAGACCT TAAAGTGCCT 
GCCAAAGCCA AGCTCACCAG ACTAAGGATG CCCTCTCAGT CGGTGGAGCA GCCGTACTCC 
AATGAAGTCC ATTGCGTTGA AGAGATTCTG AAGGAAATGA CCCACTCGTG GCCACCGCCT 
CTGACAGCCA TACACACACC CAGTACAGCC GAGCCGTCCA GATTTCCTTT CCCCACAAAG 
GACCCTCTGC ACGTCAGTCC TGCAACCCAG AGCCAAAAAC AATATGACAC GCCTTCAAAA 
ACCCACCCCA ATCCCCAGCA AGGAACCTCC ATGCTGGAGG ACGACCTGCA GCTCAGCGAC 
AGTGAAGACA GCGACACGGA GCAAGCCACA GAGAAGCCTC CATCCCCACC TGCACCTCCC 
AGCGCTCCGC AGACACTTCC CGAGCCCGTG GCCTCAGCAC ATTCCAGTAG TGGGGAGTCA 
GAGAGCAGCG AGTCAGACAG TTCCTCAGAC TCTGAGAGTG AGAGCAGCTC CAGCGACAGC 
GAGGAGGAGG AAGAGAACGA GCCCCTGGAG ACCCGGGCTC CCGAGCCCGA GCCTCCGACG 
ACAAACAAAT GGCAGCTGGA CAACTGGTTG ACCAAAGTCA ACCAACCCTC AGTGCCCCTG 
GATGGCCGGG GGAGCACAGA GTCTCCACAA TGGCGCCAGG AAAGTAAGGG TGTCGCTGAG 
GGCAGCAGTG ACCAGCAGCA TCCTGATTCC AAAGACCCTC TCCCAAAGAG CTCCAGCAAG 
ACTCTCCGGG GTCCCTCTGA GGGGCCCCAG CCTGGGAAGA GGGGCTGTCC GAAATCCCCC 
GCTCAACAGG AACCCCCACC TCGGCAAACC GTGGGCAGCA AACAACCCAG GAAACCCGCC 
AAGGGCTCCG GCCAGGCAGA GCCCCAGGCC AGCTCGCAGG CAGAGAGTGA AGTAGGACCC 
CTTCCCTACG GCTCAAAGGA GCAGACTTCC AAAGACAGAC CCAAGGTGAA GACGAAAGGC 
AGGCCGCGTG CCGTGGGCAG TCGGGAGCCT AAGCCAGAGG TCCCTGCGCC CACGCCTCAG 
GCAGCCGTGC CCAGACCGAA GCCGCCTGTG CCCACCCCCA GTGAGAAGAG GAAGCACAAG 
AGCTCCACGG CCCCCTCCAA GGCGCCCTCA GCCCCCCAGC CTCCGAAGGA CAGTGCTGGG 
GACAGGAACC CAGAACACTC TGCTCTTGTC TCCTTGACTC AGAGCCAGGG TCCATCCCAC 
AGCAGCAGGG GCAGCAGTGG CAGCGTCAGG ACTAGTGGCT GCCGACAGGC TGTGATTGCC 
CAAGGGGACG GCTGTAAAGA CAAGCTCCTG TTGCCTTTGA GAGACACCAA ATTGCTCTCG 
CCGCTCAGGG ACTCCCCACC ACCGACAAGC TTGGTGGTGA AGATAACCCT AGACCTCCTC 
ACGAGGATTC CCCAGCCTCT GGGGAAGGGG AGCCGCCCCA GGAAAGCAGA AGACAAGCAG 
CTGTCAGCAG GGAAGAAGCA GGACTCGGAG ACCAAGAGCT GTGACAGCTC CAGCAGAGTG 
ACCAAGAAGA GAAAGGGTGA CCCAGAAAAA GAGCACAGTA ACAAGAGACA CAAACTGGAT 
AAGTCGCAGA CTGCGTCATC TTCATCTTCC CATACAGAAT CTTCCAGAAC AAAAGCGCCC 
AGGTCCTCCT CAGAGAACTC AAGAAAGGAA ATGCTGCCTC CAGCATCGGC GTCCTCAGTC 
TCCTCTTCCT CATCCTCCCA GAAGCCAAGC AGACCTGCAC AGAAGAGGCC AAGGCCAGAC 
GAAGACACGT GTAGCCAGGA ACCCCCCAGA AGTGCCAGCA GCACAAAGAG CAGCTCTACA 
GACCCTCCCG CTCCCAAGCA CAGAAAAGTA CAGGCCAGGG GCTCTGAACA CAAGGGATCT 
TCTGGGGATG CTGCGAATGC TGCAAATCCT TTTCCAGTGC CTTCTTTGCC GAATGGTAAC 
GCTAAACCAG GGAAGCCACA GGTGAAGTCT GACAGACAAC AAGCTGACTT TCACATGAAG 
GAGGCTAAAA AGCTGAAGTG CAAAGCAGAG ACAATGGTAG ACAAGGCTGG AAAAGCCTTC 
AAGTATTTGG AAGCCGTCCT CTCTTTTATC GAGTGTGGAA TGGCCTCAGA GTCAGAAAGC 
TCAGCCAAGT CGGCGTACGC TGTGTACTCA GAAACCATAG ACCTCATTAG GTATGTGATG 
TCACTAAAAT GCTTCTCAGA TAACACGATG CCAGCACAAG AGAAGATATT TGCTGTTTTA 
TGCTTGCGTT GCCAGTCCCT TTTGAACATG GCGATGTTTC GATGTAAGAA AGACACAGTG 
ATGAAGTATT CTCGTACTCT CAGTGAACAC TTCAAGAGTA CTTCCAAAGT GGCCCAGGCA 
CCTTCTCCAT GCACGGCAAG AAGCACAGGC GTTCCGTCCC CCCTCTCCCC AATGCCTTCT 
CCTGCCAGCT CCGTGGGGTC CCAATCAAGT GCTGGCAGCA GTATGGGGAG CGTCGGGGTG 
ACGGCCACCG TCAGTACTCC AGTCTCCATC CAGAACATGA CCTCCTCCTA TGTCACCATC 
ACATCCCATG TCCTTACGGC CTTTAGCCTT TGGGAACAGG CCGAGGCCCT CACAAGGAAG 
AACAAAGAAT TCTTCGCTCA GCTCAGCACA AAAGTGCGTG TGTTGGCCCT CAACAGCAGC 
CTGGTGGACC TGGTGCACTA CACAAGACAG GGCCTGCAGC GGCTAAAACA GTCACCCAAA 
GGCCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aff1 ( NM_001080798.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074267.1 Mus musculus AF4/FMR2 family, member 1 (Aff1), transcript variant 1, mRNA.

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CloneID	OMu20814
Clone ID Related Accession (Same CDS sequence)	NM_001080798.2
Accession Version	NM_001080798.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	AF4/FMR2 family member 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK143742.1, CD543840.1, BC131665.1, AK139900.1, EL607205.1, CF727488.1, DV649355.1, DV654133.1, AA139222.1, CV559177.1, CA528058.1, BQ554270.1 and AI836732.1. On Sep 17, 2015 this sequence version replaced NM_001080798.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAACGA GGATTCTCCC ACGAGAGAGT GGTGGAGCCT GCTTGTACAA TGAAGATAGA 
AACCTGCTTC GAATCAGAGA GAAGGAAAGA CGCAACCAAG AAGCTCACCA GGAGAAGGAG 
GCATTTCCCG AGAAGGCTCC CCTGTTTCCA GAGCCTTACA AGACTGCAAA AGGCGATGAG 
CTATCAAGTC GGATCCAGAC CATGCTGGGT GACTATGAGG AGATGAAGGA GTTCCTGAGC 
AGCAAGTCCC ACCCCCACCG CCTGGATGGC TCTGAAGACA GGCCCGGGAA GCCCAGATAT 
CCCTTAGGTC ATGACAGGGG GAACGGGGCT GCATCCAGCT CCCTCCGCAC ACATGTCTAC 
CACCAGCCTA TCCACACTTC TGCTCCCGGA TCACGTCCTG TCGGTAACAT TAGCCACAGT 
CCAAAGATGG CACAGCCAAG GATGGAGCCA AGTCTCCACA CCAAAATCTA TGATGGCCCA 
CGTCTGACTC AAGACCACCT CAGTCAGGGA CATTGTTCCA GAAAGTGTGA CCGAAGAGCT 
GAAGGAGACT CTGCTCCCGA GAGGAAGCTC TCGCCCTTGA TCTCCTCTTT GCCATCCCCG 
GTGCCCCCTC TATCACCTGT ACATTCCAGG CTGCAGGGAA CCAGCAAGGC TCACAGCAGT 
GGCGTTAGCA GTAAAAGCTG CTGTGTGGCC AAGTCTTCCA AGGACTTGGT GGCGAAGGCC 
CAAGATAAAG AGACTCCTCA TGACGGTTTG GTGGCAGTTA CCAGCCTTGG ATCAGCCCCT 
CCTCAGCCAC CTTGCCAGAC ATTTCCACCC CCTCCTCTCC CCTCAAAAAG TGCTGCAATG 
CAGCAGAAGC CCACGGCATA TGTCCGTCCC ATGGACGGTC AGGATCAGGC TCCCAGTGAG 
TCCCCCGAGC TGAAGCTGCC ACTGGAGGAC TATGGTCAGC AAAGCTTTGA GAAACCAGAC 
CTTAAAGTGC CTGCCAAAGC CAAGCTCACC AGACTAAGGA TGCCCTCTCA GTCGGTGGAG 
CAGCCGTACT CCAATGAAGT CCATTGCGTT GAAGAGATTC TGAAGGAAAT GACCCACTCG 
TGGCCACCGC CTCTGACAGC CATACACACA CCCAGTACAG CCGAGCCGTC CAGATTTCCT 
TTCCCCACAA AGGACCCTCT GCACGTCAGT CCTGCAACCC AGAGCCAAAA ACAATATGAC 
ACGCCTTCAA AAACCCACCC CAATCCCCAG CAAGGAACCT CCATGCTGGA GGACGACCTG 
CAGCTCAGCG ACAGTGAAGA CAGCGACACG GAGCAAGCCA CAGAGAAGCC TCCATCCCCA 
CCTGCACCTC CCAGCGCTCC GCAGACACTT CCCGAGCCCG TGGCCTCAGC ACATTCCAGT 
AGTGGGGAGT CAGAGAGCAG CGAGTCAGAC AGTTCCTCAG ACTCTGAGAG TGAGAGCAGC 
TCCAGCGACA GCGAGGAGGA GGAAGAGAAC GAGCCCCTGG AGACCCGGGC TCCCGAGCCC 
GAGCCTCCGA CGACAAACAA ATGGCAGCTG GACAACTGGT TGACCAAAGT CAACCAACCC 
TCAGTGCCCC TGGATGGCCG GGGGAGCACA GAGTCTCCAC AATGGCGCCA GGAAAGTAAG 
GGTGTCGCTG AGGGCAGCAG TGACCAGCAG CATCCTGATT CCAAAGACCC TCTCCCAAAG 
AGCTCCAGCA AGACTCTCCG GGGTCCCTCT GAGGGGCCCC AGCCTGGGAA GAGGGGCTGT 
CCGAAATCCC CCGCTCAACA GGAACCCCCA CCTCGGCAAA CCGTGGGCAG CAAACAACCC 
AGGAAACCCG CCAAGGGCTC CGGCCAGGCA GAGCCCCAGG CCAGCTCGCA GGCAGAGAGT 
GAAGTAGGAC CCCTTCCCTA CGGCTCAAAG GAGCAGACTT CCAAAGACAG ACCCAAGGTG 
AAGACGAAAG GCAGGCCGCG TGCCGTGGGC AGTCGGGAGC CTAAGCCAGA GGTCCCTGCG 
CCCACGCCTC AGGCAGCCGT GCCCAGACCG AAGCCGCCTG TGCCCACCCC CAGTGAGAAG 
AGGAAGCACA AGAGCTCCAC GGCCCCCTCC AAGGCGCCCT CAGCCCCCCA GCCTCCGAAG 
GACAGTGCTG GGGACAGGAA CCCAGAACAC TCTGCTCTTG TCTCCTTGAC TCAGAGCCAG 
GGTCCATCCC ACAGCAGCAG GGGCAGCAGT GGCAGCGTCA GGACTAGTGG CTGCCGACAG 
GCTGTGATTG CCCAAGGGGA CGGCTGTAAA GACAAGCTCC TGTTGCCTTT GAGAGACACC 
AAATTGCTCT CGCCGCTCAG GGACTCCCCA CCACCGACAA GCTTGGTGGT GAAGATAACC 
CTAGACCTCC TCACGAGGAT TCCCCAGCCT CTGGGGAAGG GGAGCCGCCC CAGGAAAGCA 
GAAGACAAGC AGCTGTCAGC AGGGAAGAAG CAGGACTCGG AGACCAAGAG CTGTGACAGC 
TCCAGCAGAG TGACCAAGAA GAGAAAGGGT GACCCAGAAA AAGAGCACAG TAACAAGAGA 
CACAAACTGG ATAAGTCGCA GACTGCGTCA TCTTCATCTT CCCATACAGA ATCTTCCAGA 
ACAAAAGCGC CCAGGTCCTC CTCAGAGAAC TCAAGAAAGG AAATGCTGCC TCCAGCATCG 
GCGTCCTCAG TCTCCTCTTC CTCATCCTCC CAGAAGCCAA GCAGACCTGC ACAGAAGAGG 
CCAAGGCCAG ACGAAGACAC GTGTAGCCAG GAACCCCCCA GAAGTGCCAG CAGCACAAAG 
AGCAGCTCTA CAGACCCTCC CGCTCCCAAG CACAGAAAAG TACAGGCCAG GGGCTCTGAA 
CACAAGGGAT CTTCTGGGGA TGCTGCGAAT GCTGCAAATC CTTTTCCAGT GCCTTCTTTG 
CCGAATGGTA ACGCTAAACC AGGGAAGCCA CAGGTGAAGT CTGACAGACA ACAAGCTGAC 
TTTCACATGA AGGAGGCTAA AAAGCTGAAG TGCAAAGCAG AGACAATGGT AGACAAGGCT 
GGAAAAGCCT TCAAGTATTT GGAAGCCGTC CTCTCTTTTA TCGAGTGTGG AATGGCCTCA 
GAGTCAGAAA GCTCAGCCAA GTCGGCGTAC GCTGTGTACT CAGAAACCAT AGACCTCATT 
AGGTATGTGA TGTCACTAAA ATGCTTCTCA GATAACACGA TGCCAGCACA AGAGAAGATA 
TTTGCTGTTT TATGCTTGCG TTGCCAGTCC CTTTTGAACA TGGCGATGTT TCGATGTAAG 
AAAGACACAG TGATGAAGTA TTCTCGTACT CTCAGTGAAC ACTTCAAGAG TACTTCCAAA 
GTGGCCCAGG CACCTTCTCC ATGCACGGCA AGAAGCACAG GCGTTCCGTC CCCCCTCTCC 
CCAATGCCTT CTCCTGCCAG CTCCGTGGGG TCCCAATCAA GTGCTGGCAG CAGTATGGGG 
AGCGTCGGGG TGACGGCCAC CGTCAGTACT CCAGTCTCCA TCCAGAACAT GACCTCCTCC 
TATGTCACCA TCACATCCCA TGTCCTTACG GCCTTTAGCC TTTGGGAACA GGCCGAGGCC 
CTCACAAGGA AGAACAAAGA ATTCTTCGCT CAGCTCAGCA CAAAAGTGCG TGTGTTGGCC 
CTCAACAGCA GCCTGGTGGA CCTGGTGCAC TACACAAGAC AGGGCCTGCA GCGGCTAAAA 
CAGTCACCCA AAGGCCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074267.1
CDS	133..3813
Translation	METRILPRESGGACLYNEDRNLLRIREKERRNQEAHQEKEAFPEKAPLFPEPYKTAKGDELSSRIQTMLGDYEEMKEFLSSKSHPHRLDGSEDRPGKPRYPLGHDRGNGAASSSLRTHVYHQPIHTSAPGSRPVGNISHSPKMAQPRMEPSLHTKIYDGPRLTQDHLSQGHCSRKCDRRAEGDSAPERKLSPLISSLPSPVPPLSPVHSRLQGTSKAHSSGVSSKSCCVAKSSKDLVAKAQDKETPHDGLVAVTSLGSAPPQPPCQTFPPPPLPSKSAAMQQKPTAYVRPMDGQDQAPSESPELKLPLEDYGQQSFEKPDLKVPAKAKLTRLRMPSQSVEQPYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSRFPFPTKDPLHVSPATQSQKQYDTPSKTHPNPQQGTSMLEDDLQLSDSEDSDTEQATEKPPSPPAPPSAPQTLPEPVASAHSSSGESESSESDSSSDSESESSSSDSEEEEENEPLETRAPEPEPPTTNKWQLDNWLTKVNQPSVPLDGRGSTESPQWRQESKGVAEGSSDQQHPDSKDPLPKSSSKTLRGPSEGPQPGKRGCPKSPAQQEPPPRQTVGSKQPRKPAKGSGQAEPQASSQAESEVGPLPYGSKEQTSKDRPKVKTKGRPRAVGSREPKPEVPAPTPQAAVPRPKPPVPTPSEKRKHKSSTAPSKAPSAPQPPKDSAGDRNPEHSALVSLTQSQGPSHSSRGSSGSVRTSGCRQAVIAQGDGCKDKLLLPLRDTKLLSPLRDSPPPTSLVVKITLDLLTRIPQPLGKGSRPRKAEDKQLSAGKKQDSETKSCDSSSRVTKKRKGDPEKEHSNKRHKLDKSQTASSSSSHTESSRTKAPRSSSENSRKEMLPPASASSVSSSSSSQKPSRPAQKRPRPDEDTCSQEPPRSASSTKSSSTDPPAPKHRKVQARGSEHKGSSGDAANAANPFPVPSLPNGNAKPGKPQVKSDRQQADFHMKEAKKLKCKAETMVDKAGKAFKYLEAVLSFIECGMASESESSAKSAYAVYSETIDLIRYVMSLKCFSDNTMPAQEKIFAVLCLRCQSLLNMAMFRCKKDTVMKYSRTLSEHFKSTSKVAQAPSPCTARSTGVPSPLSPMPSPASSVGSQSSAGSSMGSVGVTATVSTPVSIQNMTSSYVTITSHVLTAFSLWEQAEALTRKNKEFFAQLSTKVRVLALNSSLVDLVHYTRQGLQRLKQSPKGP

Target ORF information:

RefSeq Version	NM_001080798.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus AF4/FMR2 family, member 1 (Aff1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080798.2

ORF Insert Sequence:

ATGGAAACGA GGATTCTCCC ACGAGAGAGT GGTGGAGCCT GCTTGTACAA TGAAGATAGA 
AACCTGCTTC GAATCAGAGA GAAGGAAAGA CGCAACCAAG AAGCTCACCA GGAGAAGGAG 
GCATTTCCCG AGAAGGCTCC CCTGTTTCCA GAGCCTTACA AGACTGCAAA AGGCGATGAG 
CTATCAAGTC GGATCCAGAC CATGCTGGGT GACTATGAGG AGATGAAGGA GTTCCTGAGC 
AGCAAGTCCC ACCCCCACCG CCTGGATGGC TCTGAAGACA GGCCCGGGAA GCCCAGATAT 
CCCTTAGGTC ATGACAGGGG GAACGGGGCT GCATCCAGCT CCCTCCGCAC ACATGTCTAC 
CACCAGCCTA TCCACACTTC TGCTCCCGGA TCACGTCCTG TCGGTAACAT TAGCCACAGT 
CCAAAGATGG CACAGCCAAG GATGGAGCCA AGTCTCCACA CCAAAATCTA TGATGGCCCA 
CGTCTGACTC AAGACCACCT CAGTCAGGGA CATTGTTCCA GAAAGTGTGA CCGAAGAGCT 
GAAGGAGACT CTGCTCCCGA GAGGAAGCTC TCGCCCTTGA TCTCCTCTTT GCCATCCCCG 
GTGCCCCCTC TATCACCTGT ACATTCCAGG CTGCAGGGAA CCAGCAAGGC TCACAGCAGT 
GGCGTTAGCA GTAAAAGCTG CTGTGTGGCC AAGTCTTCCA AGGACTTGGT GGCGAAGGCC 
CAAGATAAAG AGACTCCTCA TGACGGTTTG GTGGCAGTTA CCAGCCTTGG ATCAGCCCCT 
CCTCAGCCAC CTTGCCAGAC ATTTCCACCC CCTCCTCTCC CCTCAAAAAG TGCTGCAATG 
CAGCAGAAGC CCACGGCATA TGTCCGTCCC ATGGACGGTC AGGATCAGGC TCCCAGTGAG 
TCCCCCGAGC TGAAGCTGCC ACTGGAGGAC TATGGTCAGC AAAGCTTTGA GAAACCAGAC 
CTTAAAGTGC CTGCCAAAGC CAAGCTCACC AGACTAAGGA TGCCCTCTCA GTCGGTGGAG 
CAGCCGTACT CCAATGAAGT CCATTGCGTT GAAGAGATTC TGAAGGAAAT GACCCACTCG 
TGGCCACCGC CTCTGACAGC CATACACACA CCCAGTACAG CCGAGCCGTC CAGATTTCCT 
TTCCCCACAA AGGACCCTCT GCACGTCAGT CCTGCAACCC AGAGCCAAAA ACAATATGAC 
ACGCCTTCAA AAACCCACCC CAATCCCCAG CAAGGAACCT CCATGCTGGA GGACGACCTG 
CAGCTCAGCG ACAGTGAAGA CAGCGACACG GAGCAAGCCA CAGAGAAGCC TCCATCCCCA 
CCTGCACCTC CCAGCGCTCC GCAGACACTT CCCGAGCCCG TGGCCTCAGC ACATTCCAGT 
AGTGGGGAGT CAGAGAGCAG CGAGTCAGAC AGTTCCTCAG ACTCTGAGAG TGAGAGCAGC 
TCCAGCGACA GCGAGGAGGA GGAAGAGAAC GAGCCCCTGG AGACCCGGGC TCCCGAGCCC 
GAGCCTCCGA CGACAAACAA ATGGCAGCTG GACAACTGGT TGACCAAAGT CAACCAACCC 
TCAGTGCCCC TGGATGGCCG GGGGAGCACA GAGTCTCCAC AATGGCGCCA GGAAAGTAAG 
GGTGTCGCTG AGGGCAGCAG TGACCAGCAG CATCCTGATT CCAAAGACCC TCTCCCAAAG 
AGCTCCAGCA AGACTCTCCG GGGTCCCTCT GAGGGGCCCC AGCCTGGGAA GAGGGGCTGT 
CCGAAATCCC CCGCTCAACA GGAACCCCCA CCTCGGCAAA CCGTGGGCAG CAAACAACCC 
AGGAAACCCG CCAAGGGCTC CGGCCAGGCA GAGCCCCAGG CCAGCTCGCA GGCAGAGAGT 
GAAGTAGGAC CCCTTCCCTA CGGCTCAAAG GAGCAGACTT CCAAAGACAG ACCCAAGGTG 
AAGACGAAAG GCAGGCCGCG TGCCGTGGGC AGTCGGGAGC CTAAGCCAGA GGTCCCTGCG 
CCCACGCCTC AGGCAGCCGT GCCCAGACCG AAGCCGCCTG TGCCCACCCC CAGTGAGAAG 
AGGAAGCACA AGAGCTCCAC GGCCCCCTCC AAGGCGCCCT CAGCCCCCCA GCCTCCGAAG 
GACAGTGCTG GGGACAGGAA CCCAGAACAC TCTGCTCTTG TCTCCTTGAC TCAGAGCCAG 
GGTCCATCCC ACAGCAGCAG GGGCAGCAGT GGCAGCGTCA GGACTAGTGG CTGCCGACAG 
GCTGTGATTG CCCAAGGGGA CGGCTGTAAA GACAAGCTCC TGTTGCCTTT GAGAGACACC 
AAATTGCTCT CGCCGCTCAG GGACTCCCCA CCACCGACAA GCTTGGTGGT GAAGATAACC 
CTAGACCTCC TCACGAGGAT TCCCCAGCCT CTGGGGAAGG GGAGCCGCCC CAGGAAAGCA 
GAAGACAAGC AGCTGTCAGC AGGGAAGAAG CAGGACTCGG AGACCAAGAG CTGTGACAGC 
TCCAGCAGAG TGACCAAGAA GAGAAAGGGT GACCCAGAAA AAGAGCACAG TAACAAGAGA 
CACAAACTGG ATAAGTCGCA GACTGCGTCA TCTTCATCTT CCCATACAGA ATCTTCCAGA 
ACAAAAGCGC CCAGGTCCTC CTCAGAGAAC TCAAGAAAGG AAATGCTGCC TCCAGCATCG 
GCGTCCTCAG TCTCCTCTTC CTCATCCTCC CAGAAGCCAA GCAGACCTGC ACAGAAGAGG 
CCAAGGCCAG ACGAAGACAC GTGTAGCCAG GAACCCCCCA GAAGTGCCAG CAGCACAAAG 
AGCAGCTCTA CAGACCCTCC CGCTCCCAAG CACAGAAAAG TACAGGCCAG GGGCTCTGAA 
CACAAGGGAT CTTCTGGGGA TGCTGCGAAT GCTGCAAATC CTTTTCCAGT GCCTTCTTTG 
CCGAATGGTA ACGCTAAACC AGGGAAGCCA CAGGTGAAGT CTGACAGACA ACAAGCTGAC 
TTTCACATGA AGGAGGCTAA AAAGCTGAAG TGCAAAGCAG AGACAATGGT AGACAAGGCT 
GGAAAAGCCT TCAAGTATTT GGAAGCCGTC CTCTCTTTTA TCGAGTGTGG AATGGCCTCA 
GAGTCAGAAA GCTCAGCCAA GTCGGCGTAC GCTGTGTACT CAGAAACCAT AGACCTCATT 
AGGTATGTGA TGTCACTAAA ATGCTTCTCA GATAACACGA TGCCAGCACA AGAGAAGATA 
TTTGCTGTTT TATGCTTGCG TTGCCAGTCC CTTTTGAACA TGGCGATGTT TCGATGTAAG 
AAAGACACAG TGATGAAGTA TTCTCGTACT CTCAGTGAAC ACTTCAAGAG TACTTCCAAA 
GTGGCCCAGG CACCTTCTCC ATGCACGGCA AGAAGCACAG GCGTTCCGTC CCCCCTCTCC 
CCAATGCCTT CTCCTGCCAG CTCCGTGGGG TCCCAATCAA GTGCTGGCAG CAGTATGGGG 
AGCGTCGGGG TGACGGCCAC CGTCAGTACT CCAGTCTCCA TCCAGAACAT GACCTCCTCC 
TATGTCACCA TCACATCCCA TGTCCTTACG GCCTTTAGCC TTTGGGAACA GGCCGAGGCC 
CTCACAAGGA AGAACAAAGA ATTCTTCGCT CAGCTCAGCA CAAAAGTGCG TGTGTTGGCC 
CTCAACAGCA GCCTGGTGGA CCTGGTGCAC TACACAAGAC AGGGCCTGCA GCGGCTAAAA 
CAGTCACCCA AAGGCCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aff1 ( XM_006534801.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534864.1 Mus musculus AF4/FMR2 family, member 1 (Aff1), transcript variant X1, mRNA.

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CloneID	OMu35385
Clone ID Related Accession (Same CDS sequence)	XM_006534801.3 , XM_006534801.4
Accession Version	XM_006534801.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	AF4/FMR2 family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534801.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTTA AGGACAGCAG CAGCAGCAGC TTGTACAATG AAGATAGAAA CCTGCTTCGA 
ATCAGAGAGA AGGAAAGACG CAACCAAGAA GCTCACCAGG AGAAGGAGGC ATTTCCCGAG 
AAGGCTCCCC TGTTTCCAGA GCCTTACAAG ACTGCAAAAG GCGATGAGCT ATCAAGTCGG 
ATCCAGACCA TGCTGGGTGA CTATGAGGAG ATGAAGGAGT TCCTGAGCAG CAAGTCCCAC 
CCCCACCGCC TGGATGGCTC TGAAGACAGG CCCGGGAAGC CCAGATATCC CTTAGGTCAT 
GACAGGGGGA ACGGGGCTGC ATCCAGCTCC CTCCGCACAC ATGTCTACCA CCAGCCTATC 
CACACTTCTG CTCCCGGATC ACGTCCTGTC GGTAACATTA GCCACAGTCC AAAGATGGCA 
CAGCCAAGGA TGGAGCCAAG TCTCCACACC AAAATCTATG ATGGCCCACG TCTGACTCAA 
GACCACCTCA GTCAGGGACA TTGTTCCAGA AAGTGTGACC GAAGAGCTGA AGGAGACTCT 
GCTCCCGAGA GGAAGCTCTC GCCCTTGATC TCCTCTTTGC CATCCCCGGT GCCCCCTCTA 
TCACCTGTAC ATTCCAGGCT GCAGGGAACC AGCAAGGCTC ACAGCAGTGG CGTTAGCAGT 
AAAAGCTGCT GTGTGGCCAA GTCTTCCAAG GACTTGGTGG CGAAGGCCCA AGATAAAGAG 
ACTCCTCATG ACGGTTTGGT GGCAGTTACC AGCCTTGGAT CAGCCCCTCC TCAGCCACCT 
TGCCAGACAT TTCCACCCCC TCCTCTCCCC TCAAAAAGTG CTGCAATGCA GCAGAAGCCC 
ACGGCATATG TCCGTCCCAT GGACGGTCAG GATCAGGCTC CCAGTGAGTC CCCCGAGCTG 
AAGCTGCCAC TGGAGGACTA TGGTCAGCAA AGCTTTGAGA AACCAGACCT TAAAGTGCCT 
GCCAAAGCCA AGCTCACCAG ACTAAGGATG CCCTCTCAGT CGGTGGAGCA GCCGTACTCC 
AATGAAGTCC ATTGCGTTGA AGAGATTCTG AAGGAAATGA CCCACTCGTG GCCACCGCCT 
CTGACAGCCA TACACACACC CAGTACAGCC GAGCCGTCCA GATTTCCTTT CCCCACAAAG 
GACCCTCTGC ACGTCAGTCC TGCAACCCAG AGCCAAAAAC AATATGACAC GCCTTCAAAA 
ACCCACCCCA ATCCCCAGCA AGGAACCTCC ATGCTGGAGG ACGACCTGCA GCTCAGCGAC 
AGTGAAGACA GCGACACGGA GCAAGCCACA GAGAAGCCTC CATCCCCACC TGCACCTCCC 
AGCGCTCCGC AGACACTTCC CGAGCCCGTG GCCTCAGCAC ATTCCAGTAG TGGGGAGTCA 
GAGAGCAGCG AGTCAGACAG TTCCTCAGAC TCTGAGAGTG AGAGCAGCTC CAGCGACAGC 
GAGGAGGAGG AAGAGAACGA GCCCCTGGAG ACCCGGGCTC CCGAGCCCGA GCCTCCGACG 
ACAAACAAAT GGCAGCTGGA CAACTGGTTG ACCAAAGTCA ACCAACCCTC AGTGCCCCTG 
GATGGCCGGG GGAGCACAGA GTCTCCACAA TGGCGCCAGG AAAGTAAGGG TGTCGCTGAG 
GGCAGCAGTG ACCAGCAGCA TCCTGATTCC AAAGACCCTC TCCCAAAGAG CTCCAGCAAG 
ACTCTCCGGG GTCCCTCTGA GGGGCCCCAG CCTGGGAAGA GGGGCTGTCC GAAATCCCCC 
GCTCAACAGG AACCCCCACC TCGGCAAACC GTGGGCAGCA AACAACCCAG GAAACCCGCC 
AAGGGCTCCG GCCAGGCAGA GCCCCAGGCC AGCTCGCAGG CAGAGAGTGA AGTAGGACCC 
CTTCCCTACG GCTCAAAGGA GCAGACTTCC AAAGACAGAC CCAAGGTGAA GACGAAAGGC 
AGGCCGCGTG CCGTGGGCAG TCGGGAGCCT AAGCCAGAGG TCCCTGCGCC CACGCCTCAG 
GCAGCCGTGC CCAGACCGAA GCCGCCTGTG CCCACCCCCA GTGAGAAGAG GAAGCACAAG 
AGCTCCACGG CCCCCTCCAA GGCGCCCTCA GCCCCCCAGC CTCCGAAGGA CAGTGCTGGG 
GACAGGAACC CAGAACACTC TGCTCTTGTC TCCTTGACTC AGAGCCAGGG TCCATCCCAC 
AGCAGCAGGG GCAGCAGTGG CAGCGTCAGG ACTAGTGGCT GCCGACAGGC TGTGATTGCC 
CAAGGGGACG GCTGTAAAGA CAAGCTCCTG TTGCCTTTGA GAGACACCAA ATTGCTCTCG 
CCGCTCAGGG ACTCCCCACC ACCGACAAGC TTGGTGGTGA AGATAACCCT AGACCTCCTC 
ACGAGGATTC CCCAGCCTCT GGGGAAGGGG AGCCGCCCCA GGAAAGCAGA AGACAAGCAG 
CTGTCAGCAG GGAAGAAGCA GGACTCGGAG ACCAAGAGCT GTGACAGCTC CAGCAGAGTG 
ACCAAGAAGA GAAAGGGTGA CCCAGAAAAA GAGCACAGTA ACAAGAGACA CAAACTGGAT 
AAGTCGCAGA CTGCGTCATC TTCATCTTCC CATACAGAAT CTTCCAGAAC AAAAGCGCCC 
AGGTCCTCCT CAGAGAACTC AAGAAAGGAA ATGCTGCCTC CAGCATCGGC GTCCTCAGTC 
TCCTCTTCCT CATCCTCCCA GAAGCCAAGC AGACCTGCAC AGAAGAGGCC AAGGCCAGAC 
GAAGACACGT GTAGCCAGGA ACCCCCCAGA AGTGCCAGCA GCACAAAGAG CAGCTCTACA 
GACCCTCCCG CTCCCAAGCA CAGAAAAGTA CAGGCCAGGG GCTCTGAACA CAAGGGATCT 
TCTGGGGATG CTGCGAATGC TGCAAATCCT TTTCCAGTGC CTTCTTTGCC GAATGGTAAC 
GCTAAACCAG GGAAGCCACA GGTGAAGTCT GACAGACAAC AAGCTGACTT TCACATGAAG 
GAGGCTAAAA AGCTGAAGTG CAAAGCAGAG ACAATGGTAG ACAAGGCTGG AAAAGCCTTC 
AAGTATTTGG AAGCCGTCCT CTCTTTTATC GAGTGTGGAA TGGCCTCAGA GTCAGAAAGC 
TCAGCCAAGT CGGCGTACGC TGTGTACTCA GAAACCATAG ACCTCATTAG GTATGTGATG 
TCACTAAAAT GCTTCTCAGA TAACACGATG CCAGCACAAG AGAAGATATT TGCTGTTTTA 
TGCTTGCGTT GCCAGTCCCT TTTGAACATG GCGATGTTTC GATGTAAGAA AGACACAGTG 
ATGAAGTATT CTCGTACTCT CAGTGAACAC TTCAAGAGTA CTTCCAAAGT GGCCCAGGCA 
CCTTCTCCAT GCACGGCAAG AAGCACAGGC GTTCCGTCCC CCCTCTCCCC AATGCCTTCT 
CCTGCCAGCT CCGTGGGGTC CCAATCAAGT GCTGGCAGCA GTATGGGGAG CGTCGGGGTG 
ACGGCCACCG TCAGTACTCC AGTCTCCATC CAGAACATGA CCTCCTCCTA TGTCACCATC 
ACATCCCATG TCCTTACGGC CTTTAGCCTT TGGGAACAGG CCGAGGCCCT CACAAGGAAG 
AACAAAGAAT TCTTCGCTCA GCTCAGCACA AAAGTGCGTG TGTTGGCCCT CAACAGCAGC 
CTGGTGGACC TGGTGCACTA CACAAGACAG GGCCTGCAGC GGCTAAAACA GTCACCCAAA 
GGCCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534864.1
CDS	299..3967
Translation	MAFKDSSSSSLYNEDRNLLRIREKERRNQEAHQEKEAFPEKAPLFPEPYKTAKGDELSSRIQTMLGDYEEMKEFLSSKSHPHRLDGSEDRPGKPRYPLGHDRGNGAASSSLRTHVYHQPIHTSAPGSRPVGNISHSPKMAQPRMEPSLHTKIYDGPRLTQDHLSQGHCSRKCDRRAEGDSAPERKLSPLISSLPSPVPPLSPVHSRLQGTSKAHSSGVSSKSCCVAKSSKDLVAKAQDKETPHDGLVAVTSLGSAPPQPPCQTFPPPPLPSKSAAMQQKPTAYVRPMDGQDQAPSESPELKLPLEDYGQQSFEKPDLKVPAKAKLTRLRMPSQSVEQPYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSRFPFPTKDPLHVSPATQSQKQYDTPSKTHPNPQQGTSMLEDDLQLSDSEDSDTEQATEKPPSPPAPPSAPQTLPEPVASAHSSSGESESSESDSSSDSESESSSSDSEEEEENEPLETRAPEPEPPTTNKWQLDNWLTKVNQPSVPLDGRGSTESPQWRQESKGVAEGSSDQQHPDSKDPLPKSSSKTLRGPSEGPQPGKRGCPKSPAQQEPPPRQTVGSKQPRKPAKGSGQAEPQASSQAESEVGPLPYGSKEQTSKDRPKVKTKGRPRAVGSREPKPEVPAPTPQAAVPRPKPPVPTPSEKRKHKSSTAPSKAPSAPQPPKDSAGDRNPEHSALVSLTQSQGPSHSSRGSSGSVRTSGCRQAVIAQGDGCKDKLLLPLRDTKLLSPLRDSPPPTSLVVKITLDLLTRIPQPLGKGSRPRKAEDKQLSAGKKQDSETKSCDSSSRVTKKRKGDPEKEHSNKRHKLDKSQTASSSSSHTESSRTKAPRSSSENSRKEMLPPASASSVSSSSSSQKPSRPAQKRPRPDEDTCSQEPPRSASSTKSSSTDPPAPKHRKVQARGSEHKGSSGDAANAANPFPVPSLPNGNAKPGKPQVKSDRQQADFHMKEAKKLKCKAETMVDKAGKAFKYLEAVLSFIECGMASESESSAKSAYAVYSETIDLIRYVMSLKCFSDNTMPAQEKIFAVLCLRCQSLLNMAMFRCKKDTVMKYSRTLSEHFKSTSKVAQAPSPCTARSTGVPSPLSPMPSPASSVGSQSSAGSSMGSVGVTATVSTPVSIQNMTSSYVTITSHVLTAFSLWEQAEALTRKNKEFFAQLSTKVRVLALNSSLVDLVHYTRQGLQRLKQSPKGP

Target ORF information:

RefSeq Version	XM_006534801.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus AF4/FMR2 family, member 1 (Aff1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534801.3

ORF Insert Sequence:

ATGGCTTTTA AGGACAGCAG CAGCAGCAGC TTGTACAATG AAGATAGAAA CCTGCTTCGA 
ATCAGAGAGA AGGAAAGACG CAACCAAGAA GCTCACCAGG AGAAGGAGGC ATTTCCCGAG 
AAGGCTCCCC TGTTTCCAGA GCCTTACAAG ACTGCAAAAG GCGATGAGCT ATCAAGTCGG 
ATCCAGACCA TGCTGGGTGA CTATGAGGAG ATGAAGGAGT TCCTGAGCAG CAAGTCCCAC 
CCCCACCGCC TGGATGGCTC TGAAGACAGG CCCGGGAAGC CCAGATATCC CTTAGGTCAT 
GACAGGGGGA ACGGGGCTGC ATCCAGCTCC CTCCGCACAC ATGTCTACCA CCAGCCTATC 
CACACTTCTG CTCCCGGATC ACGTCCTGTC GGTAACATTA GCCACAGTCC AAAGATGGCA 
CAGCCAAGGA TGGAGCCAAG TCTCCACACC AAAATCTATG ATGGCCCACG TCTGACTCAA 
GACCACCTCA GTCAGGGACA TTGTTCCAGA AAGTGTGACC GAAGAGCTGA AGGAGACTCT 
GCTCCCGAGA GGAAGCTCTC GCCCTTGATC TCCTCTTTGC CATCCCCGGT GCCCCCTCTA 
TCACCTGTAC ATTCCAGGCT GCAGGGAACC AGCAAGGCTC ACAGCAGTGG CGTTAGCAGT 
AAAAGCTGCT GTGTGGCCAA GTCTTCCAAG GACTTGGTGG CGAAGGCCCA AGATAAAGAG 
ACTCCTCATG ACGGTTTGGT GGCAGTTACC AGCCTTGGAT CAGCCCCTCC TCAGCCACCT 
TGCCAGACAT TTCCACCCCC TCCTCTCCCC TCAAAAAGTG CTGCAATGCA GCAGAAGCCC 
ACGGCATATG TCCGTCCCAT GGACGGTCAG GATCAGGCTC CCAGTGAGTC CCCCGAGCTG 
AAGCTGCCAC TGGAGGACTA TGGTCAGCAA AGCTTTGAGA AACCAGACCT TAAAGTGCCT 
GCCAAAGCCA AGCTCACCAG ACTAAGGATG CCCTCTCAGT CGGTGGAGCA GCCGTACTCC 
AATGAAGTCC ATTGCGTTGA AGAGATTCTG AAGGAAATGA CCCACTCGTG GCCACCGCCT 
CTGACAGCCA TACACACACC CAGTACAGCC GAGCCGTCCA GATTTCCTTT CCCCACAAAG 
GACCCTCTGC ACGTCAGTCC TGCAACCCAG AGCCAAAAAC AATATGACAC GCCTTCAAAA 
ACCCACCCCA ATCCCCAGCA AGGAACCTCC ATGCTGGAGG ACGACCTGCA GCTCAGCGAC 
AGTGAAGACA GCGACACGGA GCAAGCCACA GAGAAGCCTC CATCCCCACC TGCACCTCCC 
AGCGCTCCGC AGACACTTCC CGAGCCCGTG GCCTCAGCAC ATTCCAGTAG TGGGGAGTCA 
GAGAGCAGCG AGTCAGACAG TTCCTCAGAC TCTGAGAGTG AGAGCAGCTC CAGCGACAGC 
GAGGAGGAGG AAGAGAACGA GCCCCTGGAG ACCCGGGCTC CCGAGCCCGA GCCTCCGACG 
ACAAACAAAT GGCAGCTGGA CAACTGGTTG ACCAAAGTCA ACCAACCCTC AGTGCCCCTG 
GATGGCCGGG GGAGCACAGA GTCTCCACAA TGGCGCCAGG AAAGTAAGGG TGTCGCTGAG 
GGCAGCAGTG ACCAGCAGCA TCCTGATTCC AAAGACCCTC TCCCAAAGAG CTCCAGCAAG 
ACTCTCCGGG GTCCCTCTGA GGGGCCCCAG CCTGGGAAGA GGGGCTGTCC GAAATCCCCC 
GCTCAACAGG AACCCCCACC TCGGCAAACC GTGGGCAGCA AACAACCCAG GAAACCCGCC 
AAGGGCTCCG GCCAGGCAGA GCCCCAGGCC AGCTCGCAGG CAGAGAGTGA AGTAGGACCC 
CTTCCCTACG GCTCAAAGGA GCAGACTTCC AAAGACAGAC CCAAGGTGAA GACGAAAGGC 
AGGCCGCGTG CCGTGGGCAG TCGGGAGCCT AAGCCAGAGG TCCCTGCGCC CACGCCTCAG 
GCAGCCGTGC CCAGACCGAA GCCGCCTGTG CCCACCCCCA GTGAGAAGAG GAAGCACAAG 
AGCTCCACGG CCCCCTCCAA GGCGCCCTCA GCCCCCCAGC CTCCGAAGGA CAGTGCTGGG 
GACAGGAACC CAGAACACTC TGCTCTTGTC TCCTTGACTC AGAGCCAGGG TCCATCCCAC 
AGCAGCAGGG GCAGCAGTGG CAGCGTCAGG ACTAGTGGCT GCCGACAGGC TGTGATTGCC 
CAAGGGGACG GCTGTAAAGA CAAGCTCCTG TTGCCTTTGA GAGACACCAA ATTGCTCTCG 
CCGCTCAGGG ACTCCCCACC ACCGACAAGC TTGGTGGTGA AGATAACCCT AGACCTCCTC 
ACGAGGATTC CCCAGCCTCT GGGGAAGGGG AGCCGCCCCA GGAAAGCAGA AGACAAGCAG 
CTGTCAGCAG GGAAGAAGCA GGACTCGGAG ACCAAGAGCT GTGACAGCTC CAGCAGAGTG 
ACCAAGAAGA GAAAGGGTGA CCCAGAAAAA GAGCACAGTA ACAAGAGACA CAAACTGGAT 
AAGTCGCAGA CTGCGTCATC TTCATCTTCC CATACAGAAT CTTCCAGAAC AAAAGCGCCC 
AGGTCCTCCT CAGAGAACTC AAGAAAGGAA ATGCTGCCTC CAGCATCGGC GTCCTCAGTC 
TCCTCTTCCT CATCCTCCCA GAAGCCAAGC AGACCTGCAC AGAAGAGGCC AAGGCCAGAC 
GAAGACACGT GTAGCCAGGA ACCCCCCAGA AGTGCCAGCA GCACAAAGAG CAGCTCTACA 
GACCCTCCCG CTCCCAAGCA CAGAAAAGTA CAGGCCAGGG GCTCTGAACA CAAGGGATCT 
TCTGGGGATG CTGCGAATGC TGCAAATCCT TTTCCAGTGC CTTCTTTGCC GAATGGTAAC 
GCTAAACCAG GGAAGCCACA GGTGAAGTCT GACAGACAAC AAGCTGACTT TCACATGAAG 
GAGGCTAAAA AGCTGAAGTG CAAAGCAGAG ACAATGGTAG ACAAGGCTGG AAAAGCCTTC 
AAGTATTTGG AAGCCGTCCT CTCTTTTATC GAGTGTGGAA TGGCCTCAGA GTCAGAAAGC 
TCAGCCAAGT CGGCGTACGC TGTGTACTCA GAAACCATAG ACCTCATTAG GTATGTGATG 
TCACTAAAAT GCTTCTCAGA TAACACGATG CCAGCACAAG AGAAGATATT TGCTGTTTTA 
TGCTTGCGTT GCCAGTCCCT TTTGAACATG GCGATGTTTC GATGTAAGAA AGACACAGTG 
ATGAAGTATT CTCGTACTCT CAGTGAACAC TTCAAGAGTA CTTCCAAAGT GGCCCAGGCA 
CCTTCTCCAT GCACGGCAAG AAGCACAGGC GTTCCGTCCC CCCTCTCCCC AATGCCTTCT 
CCTGCCAGCT CCGTGGGGTC CCAATCAAGT GCTGGCAGCA GTATGGGGAG CGTCGGGGTG 
ACGGCCACCG TCAGTACTCC AGTCTCCATC CAGAACATGA CCTCCTCCTA TGTCACCATC 
ACATCCCATG TCCTTACGGC CTTTAGCCTT TGGGAACAGG CCGAGGCCCT CACAAGGAAG 
AACAAAGAAT TCTTCGCTCA GCTCAGCACA AAAGTGCGTG TGTTGGCCCT CAACAGCAGC 
CTGGTGGACC TGGTGCACTA CACAAGACAG GGCCTGCAGC GGCTAAAACA GTCACCCAAA 
GGCCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Amlexanox Downregulates S100A6 to Sensitize
Cancer research77(16)4426-4433(2017 Aug)
Tamai H,Yamaguchi H,Miyake K,Takatori M,Kitano T,Yamanaka S,Yui S,Fukunaga K,Nakayama K,Inokuchi K

Cooperative gene activation by AF4 and DOT1L drives MLL-rearranged leukemia.
The Journal of clinical investigation127(5)1918-1931(2017 May)
Okuda H,Stanojevic B,Kanai A,Kawamura T,Takahashi S,Matsui H,Takaori-Kondo A,Yokoyama A

Importance of a specific amino acid pairing for murine MLL leukemias driven by MLLT1/3 or AFF1/4.
Leukemia research38(11)1309-15(2014 Nov)
Lokken AA,Achille NJ,Chang MJ,Lin JJ,Kuntimaddi A,Leach BI,Malik B,Nesbit JB,Zhang S,Bushweller JH,Zeleznik-Le NJ,Hemenway CS

Mediation of Af4 protein function in the cerebellum by Siah proteins.
Proceedings of the National Academy of Sciences of the United States of America101(41)14901-6(2004 Oct)
Oliver PL,Bitoun E,Clark J,Jones EL,Davies KE

A mutation in Af4 is predicted to cause cerebellar ataxia and cataracts in the robotic mouse.
The Journal of neuroscience : the official journal of the Society for Neuroscience23(5)1631-7(2003 Mar)
Isaacs AM,Oliver PL,Jones EL,Jeans A,Potter A,Hovik BH,Nolan PM,Vizor L,Glenister P,Simon AK,Gray IC,Spurr NK,Brown SD,Hunter AJ,Davies KE

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Aff1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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