AFF1 cDNA ORF clone, Canis lupus familiaris(dog)

The following AFF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe284110 XM_022413581.1
Latest version!
Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe284111 XM_022413583.1
Latest version!
Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe284112 XM_022413584.1
Latest version!
Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe284113 XM_022413585.1
Latest version!
Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe29733 XM_014109958.1
Latest version!
Canis lupus familiaris AF4/FMR2 family, member 1 (AFF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe29733 XM_022413582.1
Latest version!
Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe29733 XM_014109957.1
Latest version!
Canis lupus familiaris AF4/FMR2 family, member 1 (AFF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe29734 XM_005639159.2
Latest version!
Canis lupus familiaris AF4/FMR2 family, member 1 (AFF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe284110
    Clone ID Related Accession (Same CDS sequence) XM_022413581.1
    Accession Version XM_022413581.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AF4/FMR2 family member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CTGAAAGAGT CCAGAGCAGC AGTAGCAGCA GCTTGTACAA TGAAGACCGG 
    AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGAGAAAGAG
    GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCCTACA AGACAGAAAA AGGTGATGAG
    CTATCCAGTC GAATACAGAA CATGTTGGGA AACTATGAAG AAGTGAAGGA GTTCTTTAGC
    AGCAAGTCCC ACCCTCATCG CTTGGATGCC TCTGAAAATA GGTTGGGAAA GCCAAGATAT
    CCTTTATTTC CTGAAAAGGG GAGCAGCATT CCATCCCACT CCTTCCACAC GAGTGCCCAC
    CACCAGCCTG TTAATACTCC TGCCTCCGGA CCACTTCCTG TTGGTAACGT AAGCCAGAAC
    CCAAAGATGG CACAGCCAAG AATGGAACCA ATGTCAAATC CTCATGTCAA AAGCTATGGC
    CCACCAGACA GCCAGCACCC AACCCAAGAT CGCCTTGGTC AGGAGGTGTG CGGCTCTGGT
    CATCACAAAA AAGGTGACCG CAGAGCTGAT GGAGACCGCT GTGCTTTGGT GACAGACTCT
    GCTCCTGAGA GGGAGCTTTC TCCCTTACTC TCCTTGCCTT CCCCAGTGCC CCCCCTGTCA
    TCTGTACATT CCAACCAGCA AGCTCTTCCC CGGACACAAG GAAGCAGCAA GATTCACAGC
    AGTAACAGTA ATAGTAAAGG CTATTGCCCA GCCAAATCTC CCAAAGACCT AGTAGCAAAG
    GTCCATGATA AAGAGACCCC TCAAGACAAT TTAGTGGCAG TTGCCGGCCT TGGGGTGGCC
    CCTCCCCAGC CACCTTCTCA GACTTTCCCC CCACCTTCCC TCCCCTCAAA AACTGTTGCG
    ATGCAGCAGA AGCCCACGGC TTACGTCCGG CCCATGGACG GTCAAGATCA GGCTCCTAGT
    GAGTCACCTG AACTGAAACC ACTGCCGGAG GACTACCGGC AACAGACCTT TGAAAAAACA
    GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACCAAACTGA AGATGCCATC TCAGTCGGGT
    GAGCAGACCT ACTCCAATGA AGTTCATTGT GTTGAAGAGA TTTTGAAGGA AATGACCCAT
    TCATGGCCAC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CTGCTGAGCC CTCCAAATTT
    CCTTTTCCCA CAAAGGATTC TCAGCACATT AGTTCTGTGA CCCAAAATCA AAAACAATAT
    GATACATCTT CAAAAACCCA CACTAATTCT CAGCAGGGAA CATCCATGCT TGAAGATGAC
    CTTCAGCTCA GTGACAGTGA GGACAGTGAC AACGAACAAA GCCCAGAGAA GCCTCCTCCT
    GCGTCTGCGC CTCCCAGGTA TTGTGTGCAT GTTGCCCCGC AGTCGCTTCC AGAAGCAGCG
    GCCTCAGCAC ATTCCAGTAG TGCCGAGTCA GAGAGCACCA GTGACTCAGA CAGCTCCTCG
    GACTCCGAGA GCGAGAGCAG CTCAAGTGAC AGCGAAGAGA ATGAGCCCCT AGCAGCTCCG
    ACTCCTGAGC CTGAGCCTCC TACAACAAAC AAATGGCAGC TGGACAACTG GCTGACCAAA
    GTCAGCCAAC CGGCAGCCCC ACCAGACACC CAGGAGGGGA GCGCAGAGCC CCCGCCTCGG
    CACCCAGACA GTAAGGGCAA GGGGGGAGGC GACAGCAGCG GCCCCACCAC TGGTCACGAG
    CTCTCGGAAT CCAAAGATCC TCCCCTCAAG AGCTCCGGCA AAGCCCCCCG GGGCTCCTCC
    GAGGGCCCTC ACCCCGGCAG GAGGAGCTGT CCGAAGTCCC CGGCCCAACA GGAGGCCCTG
    CCGAGGCAGA CTGTGGGAAC CAAACAACCC AAGAAGCCGG GCAAGGCCTC CACGCCGGCG
    GACCCTCGGG CCGGCCTGCA GGTGGAGAGC GAGCCGGGCC CCCCTCCGTC CGGCTCCAGA
    GACCAGCCTT CCAAAGACAA GCCCAAGGTG AAGACGAAAG GGAGGCCGCG CGCCGGCGAC
    AGCAGGGAGC CCAAGCCCGC CGCGCCCAGC CCCGGTGACC GCAAGAAGCA CCAGAGCGGG
    CCGCTGGCCC CCTCAAAGGC ACTCTCGGGC CCCGAGCCCA CGAAGGACAA TGCGGGGGCC
    AGGAGCCCCG AGCACTTTGC CCTGGTTCCC CTGACCCAGA GCCAGGGCCC GCCCCACGGC
    GGTGGCGGCA GGACTAGTGG CTGCCGGCGC GCAGTGGTGG TCCAGGAGGA CCGCAGGAAG
    GACAAGCTCC CGGGGCCTGC CAGAGACACC AAGCTGCTGT CGCCGCTCAG GGACCCTCCT
    CCCCCACCCA TCTTGATGGT GAAGATAACC CTGGACCTTC TTTCTCGGAT ACCCCAGCTT
    CCTGGGAAAG GGGGCCGGCA GAAGAAGCCA GAGGACAAGC AGCCCCCCAC AGGGAAGAAG
    CCCGATTTGG AGAAGAGAGG CGCAGACAAC TCCAGCAAGT TGGTCAAAAA AAGAAAGGCT
    GAAGCTGAGA AAGACCATGA CAGCAAGAAA GCCAGACTGG AGAAAGAAAC CAAGTTGCAG
    TCATTATCTT CATCCCCCCA CAAAGAATCT TCCAAAACAA AAACACCCAG GCCCTCCTCT
    GAGCCCTCAA AGAAGGAGAT GCTTCCCCCC CCACCTGTGT CATCCTCATC CTCATCCTCC
    CAGAAGCCAG CCAAGCCTGC ACAAAAGAGG CCAAGGCGGG AAGCTGATCC CTCCAGCCAA
    GACGCTTCCC AAAGTGCCAG TAGTAGCAAG GGCAACCACA AAGACTCTTC CACTCCCAAG
    CACAGAAAAG TAGAGGGGAA GGGATCTGGA AGCAGTACAG AGCACAAGGG ATCTTCTGGA
    GATAGTGCAT ATCCTTTTCT AGTGCCTTCT TTGCCAAATG GTAACTCTAA ACCAGGGAAG
    CCTCCAGTGA AGCTTGACAG ACAACAAGCA GATTTTCACA GGAAGGAGGC CAAAAAGTTG
    AAGCAAAAAG CAGAGTTAAT GATGGACAAG GTTGGGAAGG CTTTTAAGTA CTTGGAGGCC
    GCCTTGTCCT TAATTGAGTG TGGGATTGCC ATGGAGTCGG AAAGCCCAGC CTCCAAATCG
    GCTTACTCGG TGTACTCCGA AACCGTAGAC CTCATTAAAT TTATAATGTC ATTAAAATCC
    TTCTCAGACG CCTCATCTCC ACAAGAGAAA ATATTTGCTG TTTTATGCAT GCGTTGTCAG
    TCCATTTTGA ACATGGCGAT GTTTCGTTGT AAAAAAGATA TAGCAATAAA GTATTCTCGT
    GCTCTTAACG AACACTTCAG CAGTTTTTTC AAAGTTGCCC AGGCACCTTC TCCATGCATT
    GCAAGAAACA CAGGCACACC GTCCCCTCTC TCTCCAGTTC CTTCTCCTGC CAGCTCCGTA
    GGGTCCCAGT CCAGTGCTGG CAGCATAGGG AGCAGTGGTA TGCCTGTCAC CATCCAAAAC
    ATGACATCTT CTTATGTCAC CATTACTTCC CATGTCCTTA CCGCCTTTGA CCTCTGGGAA
    CAGGCTGAGG TCCTTACAAG GAAGAATAAA GAATTTTTTG CTCAGCTCAG CACAAGTGTG
    TGTGCCTTGG CCCTCAACAG CAGTTTGATG GATCTGGTGC ACTATACAAG ACAGGGTTTT
    CAGCGGCTAA AACAAGCAAC CAAAACACCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269289.1
    CDS279..3971
    Translation

    Target ORF information:

    RefSeq Version XM_022413581.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413581.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CTGAAAGAGT CCAGAGCAGC AGTAGCAGCA GCTTGTACAA TGAAGACCGG 
    AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGAGAAAGAG
    GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCCTACA AGACAGAAAA AGGTGATGAG
    CTATCCAGTC GAATACAGAA CATGTTGGGA AACTATGAAG AAGTGAAGGA GTTCTTTAGC
    AGCAAGTCCC ACCCTCATCG CTTGGATGCC TCTGAAAATA GGTTGGGAAA GCCAAGATAT
    CCTTTATTTC CTGAAAAGGG GAGCAGCATT CCATCCCACT CCTTCCACAC GAGTGCCCAC
    CACCAGCCTG TTAATACTCC TGCCTCCGGA CCACTTCCTG TTGGTAACGT AAGCCAGAAC
    CCAAAGATGG CACAGCCAAG AATGGAACCA ATGTCAAATC CTCATGTCAA AAGCTATGGC
    CCACCAGACA GCCAGCACCC AACCCAAGAT CGCCTTGGTC AGGAGGTGTG CGGCTCTGGT
    CATCACAAAA AAGGTGACCG CAGAGCTGAT GGAGACCGCT GTGCTTTGGT GACAGACTCT
    GCTCCTGAGA GGGAGCTTTC TCCCTTACTC TCCTTGCCTT CCCCAGTGCC CCCCCTGTCA
    TCTGTACATT CCAACCAGCA AGCTCTTCCC CGGACACAAG GAAGCAGCAA GATTCACAGC
    AGTAACAGTA ATAGTAAAGG CTATTGCCCA GCCAAATCTC CCAAAGACCT AGTAGCAAAG
    GTCCATGATA AAGAGACCCC TCAAGACAAT TTAGTGGCAG TTGCCGGCCT TGGGGTGGCC
    CCTCCCCAGC CACCTTCTCA GACTTTCCCC CCACCTTCCC TCCCCTCAAA AACTGTTGCG
    ATGCAGCAGA AGCCCACGGC TTACGTCCGG CCCATGGACG GTCAAGATCA GGCTCCTAGT
    GAGTCACCTG AACTGAAACC ACTGCCGGAG GACTACCGGC AACAGACCTT TGAAAAAACA
    GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACCAAACTGA AGATGCCATC TCAGTCGGGT
    GAGCAGACCT ACTCCAATGA AGTTCATTGT GTTGAAGAGA TTTTGAAGGA AATGACCCAT
    TCATGGCCAC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CTGCTGAGCC CTCCAAATTT
    CCTTTTCCCA CAAAGGATTC TCAGCACATT AGTTCTGTGA CCCAAAATCA AAAACAATAT
    GATACATCTT CAAAAACCCA CACTAATTCT CAGCAGGGAA CATCCATGCT TGAAGATGAC
    CTTCAGCTCA GTGACAGTGA GGACAGTGAC AACGAACAAA GCCCAGAGAA GCCTCCTCCT
    GCGTCTGCGC CTCCCAGGTA TTGTGTGCAT GTTGCCCCGC AGTCGCTTCC AGAAGCAGCG
    GCCTCAGCAC ATTCCAGTAG TGCCGAGTCA GAGAGCACCA GTGACTCAGA CAGCTCCTCG
    GACTCCGAGA GCGAGAGCAG CTCAAGTGAC AGCGAAGAGA ATGAGCCCCT AGCAGCTCCG
    ACTCCTGAGC CTGAGCCTCC TACAACAAAC AAATGGCAGC TGGACAACTG GCTGACCAAA
    GTCAGCCAAC CGGCAGCCCC ACCAGACACC CAGGAGGGGA GCGCAGAGCC CCCGCCTCGG
    CACCCAGACA GTAAGGGCAA GGGGGGAGGC GACAGCAGCG GCCCCACCAC TGGTCACGAG
    CTCTCGGAAT CCAAAGATCC TCCCCTCAAG AGCTCCGGCA AAGCCCCCCG GGGCTCCTCC
    GAGGGCCCTC ACCCCGGCAG GAGGAGCTGT CCGAAGTCCC CGGCCCAACA GGAGGCCCTG
    CCGAGGCAGA CTGTGGGAAC CAAACAACCC AAGAAGCCGG GCAAGGCCTC CACGCCGGCG
    GACCCTCGGG CCGGCCTGCA GGTGGAGAGC GAGCCGGGCC CCCCTCCGTC CGGCTCCAGA
    GACCAGCCTT CCAAAGACAA GCCCAAGGTG AAGACGAAAG GGAGGCCGCG CGCCGGCGAC
    AGCAGGGAGC CCAAGCCCGC CGCGCCCAGC CCCGGTGACC GCAAGAAGCA CCAGAGCGGG
    CCGCTGGCCC CCTCAAAGGC ACTCTCGGGC CCCGAGCCCA CGAAGGACAA TGCGGGGGCC
    AGGAGCCCCG AGCACTTTGC CCTGGTTCCC CTGACCCAGA GCCAGGGCCC GCCCCACGGC
    GGTGGCGGCA GGACTAGTGG CTGCCGGCGC GCAGTGGTGG TCCAGGAGGA CCGCAGGAAG
    GACAAGCTCC CGGGGCCTGC CAGAGACACC AAGCTGCTGT CGCCGCTCAG GGACCCTCCT
    CCCCCACCCA TCTTGATGGT GAAGATAACC CTGGACCTTC TTTCTCGGAT ACCCCAGCTT
    CCTGGGAAAG GGGGCCGGCA GAAGAAGCCA GAGGACAAGC AGCCCCCCAC AGGGAAGAAG
    CCCGATTTGG AGAAGAGAGG CGCAGACAAC TCCAGCAAGT TGGTCAAAAA AAGAAAGGCT
    GAAGCTGAGA AAGACCATGA CAGCAAGAAA GCCAGACTGG AGAAAGAAAC CAAGTTGCAG
    TCATTATCTT CATCCCCCCA CAAAGAATCT TCCAAAACAA AAACACCCAG GCCCTCCTCT
    GAGCCCTCAA AGAAGGAGAT GCTTCCCCCC CCACCTGTGT CATCCTCATC CTCATCCTCC
    CAGAAGCCAG CCAAGCCTGC ACAAAAGAGG CCAAGGCGGG AAGCTGATCC CTCCAGCCAA
    GACGCTTCCC AAAGTGCCAG TAGTAGCAAG GGCAACCACA AAGACTCTTC CACTCCCAAG
    CACAGAAAAG TAGAGGGGAA GGGATCTGGA AGCAGTACAG AGCACAAGGG ATCTTCTGGA
    GATAGTGCAT ATCCTTTTCT AGTGCCTTCT TTGCCAAATG GTAACTCTAA ACCAGGGAAG
    CCTCCAGTGA AGCTTGACAG ACAACAAGCA GATTTTCACA GGAAGGAGGC CAAAAAGTTG
    AAGCAAAAAG CAGAGTTAAT GATGGACAAG GTTGGGAAGG CTTTTAAGTA CTTGGAGGCC
    GCCTTGTCCT TAATTGAGTG TGGGATTGCC ATGGAGTCGG AAAGCCCAGC CTCCAAATCG
    GCTTACTCGG TGTACTCCGA AACCGTAGAC CTCATTAAAT TTATAATGTC ATTAAAATCC
    TTCTCAGACG CCTCATCTCC ACAAGAGAAA ATATTTGCTG TTTTATGCAT GCGTTGTCAG
    TCCATTTTGA ACATGGCGAT GTTTCGTTGT AAAAAAGATA TAGCAATAAA GTATTCTCGT
    GCTCTTAACG AACACTTCAG CAGTTTTTTC AAAGTTGCCC AGGCACCTTC TCCATGCATT
    GCAAGAAACA CAGGCACACC GTCCCCTCTC TCTCCAGTTC CTTCTCCTGC CAGCTCCGTA
    GGGTCCCAGT CCAGTGCTGG CAGCATAGGG AGCAGTGGTA TGCCTGTCAC CATCCAAAAC
    ATGACATCTT CTTATGTCAC CATTACTTCC CATGTCCTTA CCGCCTTTGA CCTCTGGGAA
    CAGGCTGAGG TCCTTACAAG GAAGAATAAA GAATTTTTTG CTCAGCTCAG CACAAGTGTG
    TGTGCCTTGG CCCTCAACAG CAGTTTGATG GATCTGGTGC ACTATACAAG ACAGGGTTTT
    CAGCGGCTAA AACAAGCAAC CAAAACACCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284111
    Clone ID Related Accession (Same CDS sequence) XM_022413583.1
    Accession Version XM_022413583.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3672bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AF4/FMR2 family member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCTTTCT TCAAATGCAT CTTGTACAAT GAAGACCGGA ACCTGCTTCG AATTAGAGAG 
    AAGGAAAGAC GCAACCAGGA AGCTCACCAA GAGAAAGAGG CATTTTCTGA AAAGATCCCC
    CTTTTTGGAG AGCCCTACAA GACAGAAAAA GGTGATGAGC TATCCAGTCG AATACAGAAC
    ATGTTGGGAA ACTATGAAGA AGTGAAGGAG TTCTTTAGCA GCAAGTCCCA CCCTCATCGC
    TTGGATGCCT CTGAAAATAG GTTGGGAAAG CCAAGATATC CTTTATTTCC TGAAAAGGGG
    AGCAGCATTC CATCCCACTC CTTCCACACG AGTGCCCACC ACCAGCCTGT TAATACTCCT
    GCCTCCGGAC CACTTCCTGT TGGTAACGTA AGCCAGAACC CAAAGATGGC ACAGCCAAGA
    ATGGAACCAA TGTCAAATCC TCATGTCAAA AGCTATGGCC CACCAGACAG CCAGCACCCA
    ACCCAAGATC GCCTTGGTCA GGAGGTGTGC GGCTCTGGTC ATCACAAAAA AGGTGACCGC
    AGAGCTGATG GAGACCGCTG TGCTTTGGTG ACAGACTCTG CTCCTGAGAG GGAGCTTTCT
    CCCTTACTCT CCTTGCCTTC CCCAGTGCCC CCCCTGTCAT CTGTACATTC CAACCAGCAA
    GCTCTTCCCC GGACACAAGG AAGCAGCAAG ATTCACAGCA GTAACAGTAA TAGTAAAGGC
    TATTGCCCAG CCAAATCTCC CAAAGACCTA GTAGCAAAGG TCCATGATAA AGAGACCCCT
    CAAGACAATT TAGTGGCAGT TGCCGGCCTT GGGGTGGCCC CTCCCCAGCC ACCTTCTCAG
    ACTTTCCCCC CACCTTCCCT CCCCTCAAAA ACTGTTGCGA TGCAGCAGAA GCCCACGGCT
    TACGTCCGGC CCATGGACGG TCAAGATCAG GCTCCTAGTG AGTCACCTGA ACTGAAACCA
    CTGCCGGAGG ACTACCGGCA ACAGACCTTT GAAAAAACAG ACTTAAAAGT GCCTGCCAAA
    GCCAAGCTCA CCAAACTGAA GATGCCATCT CAGTCGGGTG AGCAGACCTA CTCCAATGAA
    GTTCATTGTG TTGAAGAGAT TTTGAAGGAA ATGACCCATT CATGGCCACC TCCTTTGACA
    GCAATACACA CGCCTAGTAC TGCTGAGCCC TCCAAATTTC CTTTTCCCAC AAAGGATTCT
    CAGCACATTA GTTCTGTGAC CCAAAATCAA AAACAATATG ATACATCTTC AAAAACCCAC
    ACTAATTCTC AGCAGGGAAC ATCCATGCTT GAAGATGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA ACGAACAAAG CCCAGAGAAG CCTCCTCCTG CGTCTGCGCC TCCCAGGTAT
    TGTGTGCATG TTGCCCCGCA GTCGCTTCCA GAAGCAGCGG CCTCAGCACA TTCCAGTAGT
    GCCGAGTCAG AGAGCACCAG TGACTCAGAC AGCTCCTCGG ACTCCGAGAG CGAGAGCAGC
    TCAAGTGACA GCGAAGAGAA TGAGCCCCTA GCAGCTCCGA CTCCTGAGCC TGAGCCTCCT
    ACAACAAACA AATGGCAGCT GGACAACTGG CTGACCAAAG TCAGCCAACC GGCAGCCCCA
    CCAGACACCC AGGAGGGGAG CGCAGAGCCC CCGCCTCGGC ACCCAGACAG TAAGGGCAAG
    GGGGGAGGCG ACAGCAGCGG CCCCACCACT GGTCACGAGC TCTCGGAATC CAAAGATCCT
    CCCCTCAAGA GCTCCGGCAA AGCCCCCCGG GGCTCCTCCG AGGGCCCTCA CCCCGGCAGG
    AGGAGCTGTC CGAAGTCCCC GGCCCAACAG GAGGCCCTGC CGAGGCAGAC TGTGGGAACC
    AAACAACCCA AGAAGCCGGG CAAGGCCTCC ACGCCGGCGG ACCCTCGGGC CGGCCTGCAG
    GTGGAGAGCG AGCCGGGCCC CCCTCCGTCC GGCTCCAGAG ACCAGCCTTC CAAAGACAAG
    CCCAAGGTGA AGACGAAAGG GAGGCCGCGC GCCGGCGACA GCAGGGAGCC CAAGCCCGCC
    GCGCCCAGCC CCGGTGACCG CAAGAAGCAC CAGAGCGGGC CGCTGGCCCC CTCAAAGGCA
    CTCTCGGGCC CCGAGCCCAC GAAGGACAAT GCGGGGGCCA GGAGCCCCGA GCACTTTGCC
    CTGGTTCCCC TGACCCAGAG CCAGGGCCCG CCCCACGGCG GTGGCGGCAG GACTAGTGGC
    TGCCGGCGCG CAGTGGTGGT CCAGGAGGAC CGCAGGAAGG ACAAGCTCCC GGGGCCTGCC
    AGAGACACCA AGCTGCTGTC GCCGCTCAGG GACCCTCCTC CCCCACCCAT CTTGATGGTG
    AAGATAACCC TGGACCTTCT TTCTCGGATA CCCCAGCTTC CTGGGAAAGG GGGCCGGCAG
    AAGAAGCCAG AGGACAAGCA GCCCCCCACA GGGAAGAAGC CCGATTTGGA GAAGAGAGGC
    GCAGACAACT CCAGCAAGTT GGTCAAAAAA AGAAAGGCTG AAGCTGAGAA AGACCATGAC
    AGCAAGAAAG CCAGACTGGA GAAAGAAACC AAGTTGCAGT CATTATCTTC ATCCCCCCAC
    AAAGAATCTT CCAAAACAAA AACACCCAGG CCCTCCTCTG AGCCCTCAAA GAAGGAGATG
    CTTCCCCCCC CACCTGTGTC ATCCTCATCC TCATCCTCCC AGAAGCCAGC CAAGCCTGCA
    CAAAAGAGGC CAAGGCGGGA AGCTGATCCC TCCAGCCAAG ACGCTTCCCA AAGTGCCAGT
    AGTAGCAAGG GCAACCACAA AGACTCTTCC ACTCCCAAGC ACAGAAAAGT AGAGGGGAAG
    GGATCTGGAA GCAGTACAGA GCACAAGGGA TCTTCTGGAG ATAGTGCATA TCCTTTTCTA
    GTGCCTTCTT TGCCAAATGG TAACTCTAAA CCAGGGAAGC CTCCAGTGAA GCTTGACAGA
    CAACAAGCAG ATTTTCACAG GAAGGAGGCC AAAAAGTTGA AGCAAAAAGC AGAGTTAATG
    ATGGACAAGG TTGGGAAGGC TTTTAAGTAC TTGGAGGCCG CCTTGTCCTT AATTGAGTGT
    GGGATTGCCA TGGAGTCGGA AAGCCCAGCC TCCAAATCGG CTTACTCGGT GTACTCCGAA
    ACCGTAGACC TCATTAAATT TATAATGTCA TTAAAATCCT TCTCAGACGC CTCATCTCCA
    CAAGAGAAAA TATTTGCTGT TTTATGCATG CGTTGTCAGT CCATTTTGAA CATGGCGATG
    TTTCGTTGTA AAAAAGATAT AGCAATAAAG TATTCTCGTG CTCTTAACGA ACACTTCAGC
    AGTTTTTTCA AAGTTGCCCA GGCACCTTCT CCATGCATTG CAAGAAACAC AGGCACACCG
    TCCCCTCTCT CTCCAGTTCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC CAGTGCTGGC
    AGCATAGGGA GCAGTGGTAT GCCTGTCACC ATCCAAAACA TGACATCTTC TTATGTCACC
    ATTACTTCCC ATGTCCTTAC CGCCTTTGAC CTCTGGGAAC AGGCTGAGGT CCTTACAAGG
    AAGAATAAAG AATTTTTTGC TCAGCTCAGC ACAAGTGTGT GTGCCTTGGC CCTCAACAGC
    AGTTTGATGG ATCTGGTGCA CTATACAAGA CAGGGTTTTC AGCGGCTAAA ACAAGCAACC
    AAAACACCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269291.1
    CDS109..3780
    Translation

    Target ORF information:

    RefSeq Version XM_022413583.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413583.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCTTTCT TCAAATGCAT CTTGTACAAT GAAGACCGGA ACCTGCTTCG AATTAGAGAG 
    AAGGAAAGAC GCAACCAGGA AGCTCACCAA GAGAAAGAGG CATTTTCTGA AAAGATCCCC
    CTTTTTGGAG AGCCCTACAA GACAGAAAAA GGTGATGAGC TATCCAGTCG AATACAGAAC
    ATGTTGGGAA ACTATGAAGA AGTGAAGGAG TTCTTTAGCA GCAAGTCCCA CCCTCATCGC
    TTGGATGCCT CTGAAAATAG GTTGGGAAAG CCAAGATATC CTTTATTTCC TGAAAAGGGG
    AGCAGCATTC CATCCCACTC CTTCCACACG AGTGCCCACC ACCAGCCTGT TAATACTCCT
    GCCTCCGGAC CACTTCCTGT TGGTAACGTA AGCCAGAACC CAAAGATGGC ACAGCCAAGA
    ATGGAACCAA TGTCAAATCC TCATGTCAAA AGCTATGGCC CACCAGACAG CCAGCACCCA
    ACCCAAGATC GCCTTGGTCA GGAGGTGTGC GGCTCTGGTC ATCACAAAAA AGGTGACCGC
    AGAGCTGATG GAGACCGCTG TGCTTTGGTG ACAGACTCTG CTCCTGAGAG GGAGCTTTCT
    CCCTTACTCT CCTTGCCTTC CCCAGTGCCC CCCCTGTCAT CTGTACATTC CAACCAGCAA
    GCTCTTCCCC GGACACAAGG AAGCAGCAAG ATTCACAGCA GTAACAGTAA TAGTAAAGGC
    TATTGCCCAG CCAAATCTCC CAAAGACCTA GTAGCAAAGG TCCATGATAA AGAGACCCCT
    CAAGACAATT TAGTGGCAGT TGCCGGCCTT GGGGTGGCCC CTCCCCAGCC ACCTTCTCAG
    ACTTTCCCCC CACCTTCCCT CCCCTCAAAA ACTGTTGCGA TGCAGCAGAA GCCCACGGCT
    TACGTCCGGC CCATGGACGG TCAAGATCAG GCTCCTAGTG AGTCACCTGA ACTGAAACCA
    CTGCCGGAGG ACTACCGGCA ACAGACCTTT GAAAAAACAG ACTTAAAAGT GCCTGCCAAA
    GCCAAGCTCA CCAAACTGAA GATGCCATCT CAGTCGGGTG AGCAGACCTA CTCCAATGAA
    GTTCATTGTG TTGAAGAGAT TTTGAAGGAA ATGACCCATT CATGGCCACC TCCTTTGACA
    GCAATACACA CGCCTAGTAC TGCTGAGCCC TCCAAATTTC CTTTTCCCAC AAAGGATTCT
    CAGCACATTA GTTCTGTGAC CCAAAATCAA AAACAATATG ATACATCTTC AAAAACCCAC
    ACTAATTCTC AGCAGGGAAC ATCCATGCTT GAAGATGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA ACGAACAAAG CCCAGAGAAG CCTCCTCCTG CGTCTGCGCC TCCCAGGTAT
    TGTGTGCATG TTGCCCCGCA GTCGCTTCCA GAAGCAGCGG CCTCAGCACA TTCCAGTAGT
    GCCGAGTCAG AGAGCACCAG TGACTCAGAC AGCTCCTCGG ACTCCGAGAG CGAGAGCAGC
    TCAAGTGACA GCGAAGAGAA TGAGCCCCTA GCAGCTCCGA CTCCTGAGCC TGAGCCTCCT
    ACAACAAACA AATGGCAGCT GGACAACTGG CTGACCAAAG TCAGCCAACC GGCAGCCCCA
    CCAGACACCC AGGAGGGGAG CGCAGAGCCC CCGCCTCGGC ACCCAGACAG TAAGGGCAAG
    GGGGGAGGCG ACAGCAGCGG CCCCACCACT GGTCACGAGC TCTCGGAATC CAAAGATCCT
    CCCCTCAAGA GCTCCGGCAA AGCCCCCCGG GGCTCCTCCG AGGGCCCTCA CCCCGGCAGG
    AGGAGCTGTC CGAAGTCCCC GGCCCAACAG GAGGCCCTGC CGAGGCAGAC TGTGGGAACC
    AAACAACCCA AGAAGCCGGG CAAGGCCTCC ACGCCGGCGG ACCCTCGGGC CGGCCTGCAG
    GTGGAGAGCG AGCCGGGCCC CCCTCCGTCC GGCTCCAGAG ACCAGCCTTC CAAAGACAAG
    CCCAAGGTGA AGACGAAAGG GAGGCCGCGC GCCGGCGACA GCAGGGAGCC CAAGCCCGCC
    GCGCCCAGCC CCGGTGACCG CAAGAAGCAC CAGAGCGGGC CGCTGGCCCC CTCAAAGGCA
    CTCTCGGGCC CCGAGCCCAC GAAGGACAAT GCGGGGGCCA GGAGCCCCGA GCACTTTGCC
    CTGGTTCCCC TGACCCAGAG CCAGGGCCCG CCCCACGGCG GTGGCGGCAG GACTAGTGGC
    TGCCGGCGCG CAGTGGTGGT CCAGGAGGAC CGCAGGAAGG ACAAGCTCCC GGGGCCTGCC
    AGAGACACCA AGCTGCTGTC GCCGCTCAGG GACCCTCCTC CCCCACCCAT CTTGATGGTG
    AAGATAACCC TGGACCTTCT TTCTCGGATA CCCCAGCTTC CTGGGAAAGG GGGCCGGCAG
    AAGAAGCCAG AGGACAAGCA GCCCCCCACA GGGAAGAAGC CCGATTTGGA GAAGAGAGGC
    GCAGACAACT CCAGCAAGTT GGTCAAAAAA AGAAAGGCTG AAGCTGAGAA AGACCATGAC
    AGCAAGAAAG CCAGACTGGA GAAAGAAACC AAGTTGCAGT CATTATCTTC ATCCCCCCAC
    AAAGAATCTT CCAAAACAAA AACACCCAGG CCCTCCTCTG AGCCCTCAAA GAAGGAGATG
    CTTCCCCCCC CACCTGTGTC ATCCTCATCC TCATCCTCCC AGAAGCCAGC CAAGCCTGCA
    CAAAAGAGGC CAAGGCGGGA AGCTGATCCC TCCAGCCAAG ACGCTTCCCA AAGTGCCAGT
    AGTAGCAAGG GCAACCACAA AGACTCTTCC ACTCCCAAGC ACAGAAAAGT AGAGGGGAAG
    GGATCTGGAA GCAGTACAGA GCACAAGGGA TCTTCTGGAG ATAGTGCATA TCCTTTTCTA
    GTGCCTTCTT TGCCAAATGG TAACTCTAAA CCAGGGAAGC CTCCAGTGAA GCTTGACAGA
    CAACAAGCAG ATTTTCACAG GAAGGAGGCC AAAAAGTTGA AGCAAAAAGC AGAGTTAATG
    ATGGACAAGG TTGGGAAGGC TTTTAAGTAC TTGGAGGCCG CCTTGTCCTT AATTGAGTGT
    GGGATTGCCA TGGAGTCGGA AAGCCCAGCC TCCAAATCGG CTTACTCGGT GTACTCCGAA
    ACCGTAGACC TCATTAAATT TATAATGTCA TTAAAATCCT TCTCAGACGC CTCATCTCCA
    CAAGAGAAAA TATTTGCTGT TTTATGCATG CGTTGTCAGT CCATTTTGAA CATGGCGATG
    TTTCGTTGTA AAAAAGATAT AGCAATAAAG TATTCTCGTG CTCTTAACGA ACACTTCAGC
    AGTTTTTTCA AAGTTGCCCA GGCACCTTCT CCATGCATTG CAAGAAACAC AGGCACACCG
    TCCCCTCTCT CTCCAGTTCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC CAGTGCTGGC
    AGCATAGGGA GCAGTGGTAT GCCTGTCACC ATCCAAAACA TGACATCTTC TTATGTCACC
    ATTACTTCCC ATGTCCTTAC CGCCTTTGAC CTCTGGGAAC AGGCTGAGGT CCTTACAAGG
    AAGAATAAAG AATTTTTTGC TCAGCTCAGC ACAAGTGTGT GTGCCTTGGC CCTCAACAGC
    AGTTTGATGG ATCTGGTGCA CTATACAAGA CAGGGTTTTC AGCGGCTAAA ACAAGCAACC
    AAAACACCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284112
    Clone ID Related Accession (Same CDS sequence) XM_022413584.1
    Accession Version XM_022413584.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AF4/FMR2 family member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCCC AGTCCAGCTT GTACAATGAA GACCGGAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC TCACCAAGAG AAAGAGGCAT TTTCTGAAAA GATCCCCCTT
    TTTGGAGAGC CCTACAAGAC AGAAAAAGGT GATGAGCTAT CCAGTCGAAT ACAGAACATG
    TTGGGAAACT ATGAAGAAGT GAAGGAGTTC TTTAGCAGCA AGTCCCACCC TCATCGCTTG
    GATGCCTCTG AAAATAGGTT GGGAAAGCCA AGATATCCTT TATTTCCTGA AAAGGGGAGC
    AGCATTCCAT CCCACTCCTT CCACACGAGT GCCCACCACC AGCCTGTTAA TACTCCTGCC
    TCCGGACCAC TTCCTGTTGG TAACGTAAGC CAGAACCCAA AGATGGCACA GCCAAGAATG
    GAACCAATGT CAAATCCTCA TGTCAAAAGC TATGGCCCAC CAGACAGCCA GCACCCAACC
    CAAGATCGCC TTGGTCAGGA GGTGTGCGGC TCTGGTCATC ACAAAAAAGG TGACCGCAGA
    GCTGATGGAG ACCGCTGTGC TTTGGTGACA GACTCTGCTC CTGAGAGGGA GCTTTCTCCC
    TTACTCTCCT TGCCTTCCCC AGTGCCCCCC CTGTCATCTG TACATTCCAA CCAGCAAGCT
    CTTCCCCGGA CACAAGGAAG CAGCAAGATT CACAGCAGTA ACAGTAATAG TAAAGGCTAT
    TGCCCAGCCA AATCTCCCAA AGACCTAGTA GCAAAGGTCC ATGATAAAGA GACCCCTCAA
    GACAATTTAG TGGCAGTTGC CGGCCTTGGG GTGGCCCCTC CCCAGCCACC TTCTCAGACT
    TTCCCCCCAC CTTCCCTCCC CTCAAAAACT GTTGCGATGC AGCAGAAGCC CACGGCTTAC
    GTCCGGCCCA TGGACGGTCA AGATCAGGCT CCTAGTGAGT CACCTGAACT GAAACCACTG
    CCGGAGGACT ACCGGCAACA GACCTTTGAA AAAACAGACT TAAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCATCTCAG TCGGGTGAGC AGACCTACTC CAATGAAGTT
    CATTGTGTTG AAGAGATTTT GAAGGAAATG ACCCATTCAT GGCCACCTCC TTTGACAGCA
    ATACACACGC CTAGTACTGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG
    CACATTAGTT CTGTGACCCA AAATCAAAAA CAATATGATA CATCTTCAAA AACCCACACT
    AATTCTCAGC AGGGAACATC CATGCTTGAA GATGACCTTC AGCTCAGTGA CAGTGAGGAC
    AGTGACAACG AACAAAGCCC AGAGAAGCCT CCTCCTGCGT CTGCGCCTCC CAGGTATTGT
    GTGCATGTTG CCCCGCAGTC GCTTCCAGAA GCAGCGGCCT CAGCACATTC CAGTAGTGCC
    GAGTCAGAGA GCACCAGTGA CTCAGACAGC TCCTCGGACT CCGAGAGCGA GAGCAGCTCA
    AGTGACAGCG AAGAGAATGA GCCCCTAGCA GCTCCGACTC CTGAGCCTGA GCCTCCTACA
    ACAAACAAAT GGCAGCTGGA CAACTGGCTG ACCAAAGTCA GCCAACCGGC AGCCCCACCA
    GACACCCAGG AGGGGAGCGC AGAGCCCCCG CCTCGGCACC CAGACAGTAA GGGCAAGGGG
    GGAGGCGACA GCAGCGGCCC CACCACTGGT CACGAGCTCT CGGAATCCAA AGATCCTCCC
    CTCAAGAGCT CCGGCAAAGC CCCCCGGGGC TCCTCCGAGG GCCCTCACCC CGGCAGGAGG
    AGCTGTCCGA AGTCCCCGGC CCAACAGGAG GCCCTGCCGA GGCAGACTGT GGGAACCAAA
    CAACCCAAGA AGCCGGGCAA GGCCTCCACG CCGGCGGACC CTCGGGCCGG CCTGCAGGTG
    GAGAGCGAGC CGGGCCCCCC TCCGTCCGGC TCCAGAGACC AGCCTTCCAA AGACAAGCCC
    AAGGTGAAGA CGAAAGGGAG GCCGCGCGCC GGCGACAGCA GGGAGCCCAA GCCCGCCGCG
    CCCAGCCCCG GTGACCGCAA GAAGCACCAG AGCGGGCCGC TGGCCCCCTC AAAGGCACTC
    TCGGGCCCCG AGCCCACGAA GGACAATGCG GGGGCCAGGA GCCCCGAGCA CTTTGCCCTG
    GTTCCCCTGA CCCAGAGCCA GGGCCCGCCC CACGGCGGTG GCGGCAGGAC TAGTGGCTGC
    CGGCGCGCAG TGGTGGTCCA GGAGGACCGC AGGAAGGACA AGCTCCCGGG GCCTGCCAGA
    GACACCAAGC TGCTGTCGCC GCTCAGGGAC CCTCCTCCCC CACCCATCTT GATGGTGAAG
    ATAACCCTGG ACCTTCTTTC TCGGATACCC CAGCTTCCTG GGAAAGGGGG CCGGCAGAAG
    AAGCCAGAGG ACAAGCAGCC CCCCACAGGG AAGAAGCCCG ATTTGGAGAA GAGAGGCGCA
    GACAACTCCA GCAAGTTGGT CAAAAAAAGA AAGGCTGAAG CTGAGAAAGA CCATGACAGC
    AAGAAAGCCA GACTGGAGAA AGAAACCAAG TTGCAGTCAT TATCTTCATC CCCCCACAAA
    GAATCTTCCA AAACAAAAAC ACCCAGGCCC TCCTCTGAGC CCTCAAAGAA GGAGATGCTT
    CCCCCCCCAC CTGTGTCATC CTCATCCTCA TCCTCCCAGA AGCCAGCCAA GCCTGCACAA
    AAGAGGCCAA GGCGGGAAGC TGATCCCTCC AGCCAAGACG CTTCCCAAAG TGCCAGTAGT
    AGCAAGGGCA ACCACAAAGA CTCTTCCACT CCCAAGCACA GAAAAGTAGA GGGGAAGGGA
    TCTGGAAGCA GTACAGAGCA CAAGGGATCT TCTGGAGATA GTGCATATCC TTTTCTAGTG
    CCTTCTTTGC CAAATGGTAA CTCTAAACCA GGGAAGCCTC CAGTGAAGCT TGACAGACAA
    CAAGCAGATT TTCACAGGAA GGAGGCCAAA AAGTTGAAGC AAAAAGCAGA GTTAATGATG
    GACAAGGTTG GGAAGGCTTT TAAGTACTTG GAGGCCGCCT TGTCCTTAAT TGAGTGTGGG
    ATTGCCATGG AGTCGGAAAG CCCAGCCTCC AAATCGGCTT ACTCGGTGTA CTCCGAAACC
    GTAGACCTCA TTAAATTTAT AATGTCATTA AAATCCTTCT CAGACGCCTC ATCTCCACAA
    GAGAAAATAT TTGCTGTTTT ATGCATGCGT TGTCAGTCCA TTTTGAACAT GGCGATGTTT
    CGTTGTAAAA AAGATATAGC AATAAAGTAT TCTCGTGCTC TTAACGAACA CTTCAGCAGT
    TTTTTCAAAG TTGCCCAGGC ACCTTCTCCA TGCATTGCAA GAAACACAGG CACACCGTCC
    CCTCTCTCTC CAGTTCCTTC TCCTGCCAGC TCCGTAGGGT CCCAGTCCAG TGCTGGCAGC
    ATAGGGAGCA GTGGTATGCC TGTCACCATC CAAAACATGA CATCTTCTTA TGTCACCATT
    ACTTCCCATG TCCTTACCGC CTTTGACCTC TGGGAACAGG CTGAGGTCCT TACAAGGAAG
    AATAAAGAAT TTTTTGCTCA GCTCAGCACA AGTGTGTGTG CCTTGGCCCT CAACAGCAGT
    TTGATGGATC TGGTGCACTA TACAAGACAG GGTTTTCAGC GGCTAAAACA AGCAACCAAA
    ACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269292.1
    CDS67..3735
    Translation

    Target ORF information:

    RefSeq Version XM_022413584.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413584.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCCC AGTCCAGCTT GTACAATGAA GACCGGAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC TCACCAAGAG AAAGAGGCAT TTTCTGAAAA GATCCCCCTT
    TTTGGAGAGC CCTACAAGAC AGAAAAAGGT GATGAGCTAT CCAGTCGAAT ACAGAACATG
    TTGGGAAACT ATGAAGAAGT GAAGGAGTTC TTTAGCAGCA AGTCCCACCC TCATCGCTTG
    GATGCCTCTG AAAATAGGTT GGGAAAGCCA AGATATCCTT TATTTCCTGA AAAGGGGAGC
    AGCATTCCAT CCCACTCCTT CCACACGAGT GCCCACCACC AGCCTGTTAA TACTCCTGCC
    TCCGGACCAC TTCCTGTTGG TAACGTAAGC CAGAACCCAA AGATGGCACA GCCAAGAATG
    GAACCAATGT CAAATCCTCA TGTCAAAAGC TATGGCCCAC CAGACAGCCA GCACCCAACC
    CAAGATCGCC TTGGTCAGGA GGTGTGCGGC TCTGGTCATC ACAAAAAAGG TGACCGCAGA
    GCTGATGGAG ACCGCTGTGC TTTGGTGACA GACTCTGCTC CTGAGAGGGA GCTTTCTCCC
    TTACTCTCCT TGCCTTCCCC AGTGCCCCCC CTGTCATCTG TACATTCCAA CCAGCAAGCT
    CTTCCCCGGA CACAAGGAAG CAGCAAGATT CACAGCAGTA ACAGTAATAG TAAAGGCTAT
    TGCCCAGCCA AATCTCCCAA AGACCTAGTA GCAAAGGTCC ATGATAAAGA GACCCCTCAA
    GACAATTTAG TGGCAGTTGC CGGCCTTGGG GTGGCCCCTC CCCAGCCACC TTCTCAGACT
    TTCCCCCCAC CTTCCCTCCC CTCAAAAACT GTTGCGATGC AGCAGAAGCC CACGGCTTAC
    GTCCGGCCCA TGGACGGTCA AGATCAGGCT CCTAGTGAGT CACCTGAACT GAAACCACTG
    CCGGAGGACT ACCGGCAACA GACCTTTGAA AAAACAGACT TAAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCATCTCAG TCGGGTGAGC AGACCTACTC CAATGAAGTT
    CATTGTGTTG AAGAGATTTT GAAGGAAATG ACCCATTCAT GGCCACCTCC TTTGACAGCA
    ATACACACGC CTAGTACTGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG
    CACATTAGTT CTGTGACCCA AAATCAAAAA CAATATGATA CATCTTCAAA AACCCACACT
    AATTCTCAGC AGGGAACATC CATGCTTGAA GATGACCTTC AGCTCAGTGA CAGTGAGGAC
    AGTGACAACG AACAAAGCCC AGAGAAGCCT CCTCCTGCGT CTGCGCCTCC CAGGTATTGT
    GTGCATGTTG CCCCGCAGTC GCTTCCAGAA GCAGCGGCCT CAGCACATTC CAGTAGTGCC
    GAGTCAGAGA GCACCAGTGA CTCAGACAGC TCCTCGGACT CCGAGAGCGA GAGCAGCTCA
    AGTGACAGCG AAGAGAATGA GCCCCTAGCA GCTCCGACTC CTGAGCCTGA GCCTCCTACA
    ACAAACAAAT GGCAGCTGGA CAACTGGCTG ACCAAAGTCA GCCAACCGGC AGCCCCACCA
    GACACCCAGG AGGGGAGCGC AGAGCCCCCG CCTCGGCACC CAGACAGTAA GGGCAAGGGG
    GGAGGCGACA GCAGCGGCCC CACCACTGGT CACGAGCTCT CGGAATCCAA AGATCCTCCC
    CTCAAGAGCT CCGGCAAAGC CCCCCGGGGC TCCTCCGAGG GCCCTCACCC CGGCAGGAGG
    AGCTGTCCGA AGTCCCCGGC CCAACAGGAG GCCCTGCCGA GGCAGACTGT GGGAACCAAA
    CAACCCAAGA AGCCGGGCAA GGCCTCCACG CCGGCGGACC CTCGGGCCGG CCTGCAGGTG
    GAGAGCGAGC CGGGCCCCCC TCCGTCCGGC TCCAGAGACC AGCCTTCCAA AGACAAGCCC
    AAGGTGAAGA CGAAAGGGAG GCCGCGCGCC GGCGACAGCA GGGAGCCCAA GCCCGCCGCG
    CCCAGCCCCG GTGACCGCAA GAAGCACCAG AGCGGGCCGC TGGCCCCCTC AAAGGCACTC
    TCGGGCCCCG AGCCCACGAA GGACAATGCG GGGGCCAGGA GCCCCGAGCA CTTTGCCCTG
    GTTCCCCTGA CCCAGAGCCA GGGCCCGCCC CACGGCGGTG GCGGCAGGAC TAGTGGCTGC
    CGGCGCGCAG TGGTGGTCCA GGAGGACCGC AGGAAGGACA AGCTCCCGGG GCCTGCCAGA
    GACACCAAGC TGCTGTCGCC GCTCAGGGAC CCTCCTCCCC CACCCATCTT GATGGTGAAG
    ATAACCCTGG ACCTTCTTTC TCGGATACCC CAGCTTCCTG GGAAAGGGGG CCGGCAGAAG
    AAGCCAGAGG ACAAGCAGCC CCCCACAGGG AAGAAGCCCG ATTTGGAGAA GAGAGGCGCA
    GACAACTCCA GCAAGTTGGT CAAAAAAAGA AAGGCTGAAG CTGAGAAAGA CCATGACAGC
    AAGAAAGCCA GACTGGAGAA AGAAACCAAG TTGCAGTCAT TATCTTCATC CCCCCACAAA
    GAATCTTCCA AAACAAAAAC ACCCAGGCCC TCCTCTGAGC CCTCAAAGAA GGAGATGCTT
    CCCCCCCCAC CTGTGTCATC CTCATCCTCA TCCTCCCAGA AGCCAGCCAA GCCTGCACAA
    AAGAGGCCAA GGCGGGAAGC TGATCCCTCC AGCCAAGACG CTTCCCAAAG TGCCAGTAGT
    AGCAAGGGCA ACCACAAAGA CTCTTCCACT CCCAAGCACA GAAAAGTAGA GGGGAAGGGA
    TCTGGAAGCA GTACAGAGCA CAAGGGATCT TCTGGAGATA GTGCATATCC TTTTCTAGTG
    CCTTCTTTGC CAAATGGTAA CTCTAAACCA GGGAAGCCTC CAGTGAAGCT TGACAGACAA
    CAAGCAGATT TTCACAGGAA GGAGGCCAAA AAGTTGAAGC AAAAAGCAGA GTTAATGATG
    GACAAGGTTG GGAAGGCTTT TAAGTACTTG GAGGCCGCCT TGTCCTTAAT TGAGTGTGGG
    ATTGCCATGG AGTCGGAAAG CCCAGCCTCC AAATCGGCTT ACTCGGTGTA CTCCGAAACC
    GTAGACCTCA TTAAATTTAT AATGTCATTA AAATCCTTCT CAGACGCCTC ATCTCCACAA
    GAGAAAATAT TTGCTGTTTT ATGCATGCGT TGTCAGTCCA TTTTGAACAT GGCGATGTTT
    CGTTGTAAAA AAGATATAGC AATAAAGTAT TCTCGTGCTC TTAACGAACA CTTCAGCAGT
    TTTTTCAAAG TTGCCCAGGC ACCTTCTCCA TGCATTGCAA GAAACACAGG CACACCGTCC
    CCTCTCTCTC CAGTTCCTTC TCCTGCCAGC TCCGTAGGGT CCCAGTCCAG TGCTGGCAGC
    ATAGGGAGCA GTGGTATGCC TGTCACCATC CAAAACATGA CATCTTCTTA TGTCACCATT
    ACTTCCCATG TCCTTACCGC CTTTGACCTC TGGGAACAGG CTGAGGTCCT TACAAGGAAG
    AATAAAGAAT TTTTTGCTCA GCTCAGCACA AGTGTGTGTG CCTTGGCCCT CAACAGCAGT
    TTGATGGATC TGGTGCACTA TACAAGACAG GGTTTTCAGC GGCTAAAACA AGCAACCAAA
    ACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284113
    Clone ID Related Accession (Same CDS sequence) XM_022413585.1
    Accession Version XM_022413585.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AF4/FMR2 family member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTTGGGAA ACTATGAAGA AGTGAAGGAG TTCTTTAGCA GCAAGTCCCA CCCTCATCGC 
    TTGGATGCCT CTGAAAATAG GTTGGGAAAG CCAAGATATC CTTTATTTCC TGAAAAGGGG
    AGCAGCATTC CATCCCACTC CTTCCACACG AGTGCCCACC ACCAGCCTGT TAATACTCCT
    GCCTCCGGAC CACTTCCTGT TGGTAACGTA AGCCAGAACC CAAAGATGGC ACAGCCAAGA
    ATGGAACCAA TGTCAAATCC TCATGTCAAA AGCTATGGCC CACCAGACAG CCAGCACCCA
    ACCCAAGATC GCCTTGGTCA GGAGGTGTGC GGCTCTGGTC ATCACAAAAA AGGTGACCGC
    AGAGCTGATG GAGACCGCTG TGCTTTGGTG ACAGACTCTG CTCCTGAGAG GGAGCTTTCT
    CCCTTACTCT CCTTGCCTTC CCCAGTGCCC CCCCTGTCAT CTGTACATTC CAACCAGCAA
    GCTCTTCCCC GGACACAAGG AAGCAGCAAG ATTCACAGCA GTAACAGTAA TAGTAAAGGC
    TATTGCCCAG CCAAATCTCC CAAAGACCTA GTAGCAAAGG TCCATGATAA AGAGACCCCT
    CAAGACAATT TAGTGGCAGT TGCCGGCCTT GGGGTGGCCC CTCCCCAGCC ACCTTCTCAG
    ACTTTCCCCC CACCTTCCCT CCCCTCAAAA ACTGTTGCGA TGCAGCAGAA GCCCACGGCT
    TACGTCCGGC CCATGGACGG TCAAGATCAG GCTCCTAGTG AGTCACCTGA ACTGAAACCA
    CTGCCGGAGG ACTACCGGCA ACAGACCTTT GAAAAAACAG ACTTAAAAGT GCCTGCCAAA
    GCCAAGCTCA CCAAACTGAA GATGCCATCT CAGTCGGGTG AGCAGACCTA CTCCAATGAA
    GTTCATTGTG TTGAAGAGAT TTTGAAGGAA ATGACCCATT CATGGCCACC TCCTTTGACA
    GCAATACACA CGCCTAGTAC TGCTGAGCCC TCCAAATTTC CTTTTCCCAC AAAGGATTCT
    CAGCACATTA GTTCTGTGAC CCAAAATCAA AAACAATATG ATACATCTTC AAAAACCCAC
    ACTAATTCTC AGCAGGGAAC ATCCATGCTT GAAGATGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA ACGAACAAAG CCCAGAGAAG CCTCCTCCTG CGTCTGCGCC TCCCAGGTAT
    TGTGTGCATG TTGCCCCGCA GTCGCTTCCA GAAGCAGCGG CCTCAGCACA TTCCAGTAGT
    GCCGAGTCAG AGAGCACCAG TGACTCAGAC AGCTCCTCGG ACTCCGAGAG CGAGAGCAGC
    TCAAGTGACA GCGAAGAGAA TGAGCCCCTA GCAGCTCCGA CTCCTGAGCC TGAGCCTCCT
    ACAACAAACA AATGGCAGCT GGACAACTGG CTGACCAAAG TCAGCCAACC GGCAGCCCCA
    CCAGACACCC AGGAGGGGAG CGCAGAGCCC CCGCCTCGGC ACCCAGACAG TAAGGGCAAG
    GGGGGAGGCG ACAGCAGCGG CCCCACCACT GGTCACGAGC TCTCGGAATC CAAAGATCCT
    CCCCTCAAGA GCTCCGGCAA AGCCCCCCGG GGCTCCTCCG AGGGCCCTCA CCCCGGCAGG
    AGGAGCTGTC CGAAGTCCCC GGCCCAACAG GAGGCCCTGC CGAGGCAGAC TGTGGGAACC
    AAACAACCCA AGAAGCCGGG CAAGGCCTCC ACGCCGGCGG ACCCTCGGGC CGGCCTGCAG
    GTGGAGAGCG AGCCGGGCCC CCCTCCGTCC GGCTCCAGAG ACCAGCCTTC CAAAGACAAG
    CCCAAGGTGA AGACGAAAGG GAGGCCGCGC GCCGGCGACA GCAGGGAGCC CAAGCCCGCC
    GCGCCCAGCC CCGGTGACCG CAAGAAGCAC CAGAGCGGGC CGCTGGCCCC CTCAAAGGCA
    CTCTCGGGCC CCGAGCCCAC GAAGGACAAT GCGGGGGCCA GGAGCCCCGA GCACTTTGCC
    CTGGTTCCCC TGACCCAGAG CCAGGGCCCG CCCCACGGCG GTGGCGGCAG GACTAGTGGC
    TGCCGGCGCG CAGTGGTGGT CCAGGAGGAC CGCAGGAAGG ACAAGCTCCC GGGGCCTGCC
    AGAGACACCA AGCTGCTGTC GCCGCTCAGG GACCCTCCTC CCCCACCCAT CTTGATGGTG
    AAGATAACCC TGGACCTTCT TTCTCGGATA CCCCAGCTTC CTGGGAAAGG GGGCCGGCAG
    AAGAAGCCAG AGGACAAGCA GCCCCCCACA GGGAAGAAGC CCGATTTGGA GAAGAGAGGC
    GCAGACAACT CCAGCAAGTT GGTCAAAAAA AGAAAGGCTG AAGCTGAGAA AGACCATGAC
    AGCAAGAAAG CCAGACTGGA GAAAGAAACC AAGTTGCAGT CATTATCTTC ATCCCCCCAC
    AAAGAATCTT CCAAAACAAA AACACCCAGG CCCTCCTCTG AGCCCTCAAA GAAGGAGATG
    CTTCCCCCCC CACCTGTGTC ATCCTCATCC TCATCCTCCC AGAAGCCAGC CAAGCCTGCA
    CAAAAGAGGC CAAGGCGGGA AGCTGATCCC TCCAGCCAAG ACGCTTCCCA AAGTGCCAGT
    AGTAGCAAGG GCAACCACAA AGACTCTTCC ACTCCCAAGC ACAGAAAAGT AGAGGGGAAG
    GGATCTGGAA GCAGTACAGA GCACAAGGGA TCTTCTGGAG ATAGTGCATA TCCTTTTCTA
    GTGCCTTCTT TGCCAAATGG TAACTCTAAA CCAGGGAAGC CTCCAGTGAA GCTTGACAGA
    CAACAAGCAG ATTTTCACAG GAAGGAGGCC AAAAAGTTGA AGCAAAAAGC AGAGTTAATG
    ATGGACAAGG TTGGGAAGGC TTTTAAGTAC TTGGAGGCCG CCTTGTCCTT AATTGAGTGT
    GGGATTGCCA TGGAGTCGGA AAGCCCAGCC TCCAAATCGG CTTACTCGGT GTACTCCGAA
    ACCGTAGACC TCATTAAATT TATAATGTCA TTAAAATCCT TCTCAGACGC CTCATCTCCA
    CAAGAGAAAA TATTTGCTGT TTTATGCATG CGTTGTCAGT CCATTTTGAA CATGGCGATG
    TTTCGTTGTA AAAAAGATAT AGCAATAAAG TATTCTCGTG CTCTTAACGA ACACTTCAGC
    AGTTTTTTCA AAGTTGCCCA GGCACCTTCT CCATGCATTG CAAGAAACAC AGGCACACCG
    TCCCCTCTCT CTCCAGTTCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC CAGTGCTGGC
    AGCATAGGGA GCAGTGGTAT GCCTGTCACC ATCCAAAACA TGACATCTTC TTATGTCACC
    ATTACTTCCC ATGTCCTTAC CGCCTTTGAC CTCTGGGAAC AGGCTGAGGT CCTTACAAGG
    AAGAATAAAG AATTTTTTGC TCAGCTCAGC ACAAGTGTGT GTGCCTTGGC CCTCAACAGC
    AGTTTGATGG ATCTGGTGCA CTATACAAGA CAGGGTTTTC AGCGGCTAAA ACAAGCAACC
    AAAACACCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269293.1
    CDS184..3675
    Translation

    Target ORF information:

    RefSeq Version XM_022413585.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413585.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTTGGGAA ACTATGAAGA AGTGAAGGAG TTCTTTAGCA GCAAGTCCCA CCCTCATCGC 
    TTGGATGCCT CTGAAAATAG GTTGGGAAAG CCAAGATATC CTTTATTTCC TGAAAAGGGG
    AGCAGCATTC CATCCCACTC CTTCCACACG AGTGCCCACC ACCAGCCTGT TAATACTCCT
    GCCTCCGGAC CACTTCCTGT TGGTAACGTA AGCCAGAACC CAAAGATGGC ACAGCCAAGA
    ATGGAACCAA TGTCAAATCC TCATGTCAAA AGCTATGGCC CACCAGACAG CCAGCACCCA
    ACCCAAGATC GCCTTGGTCA GGAGGTGTGC GGCTCTGGTC ATCACAAAAA AGGTGACCGC
    AGAGCTGATG GAGACCGCTG TGCTTTGGTG ACAGACTCTG CTCCTGAGAG GGAGCTTTCT
    CCCTTACTCT CCTTGCCTTC CCCAGTGCCC CCCCTGTCAT CTGTACATTC CAACCAGCAA
    GCTCTTCCCC GGACACAAGG AAGCAGCAAG ATTCACAGCA GTAACAGTAA TAGTAAAGGC
    TATTGCCCAG CCAAATCTCC CAAAGACCTA GTAGCAAAGG TCCATGATAA AGAGACCCCT
    CAAGACAATT TAGTGGCAGT TGCCGGCCTT GGGGTGGCCC CTCCCCAGCC ACCTTCTCAG
    ACTTTCCCCC CACCTTCCCT CCCCTCAAAA ACTGTTGCGA TGCAGCAGAA GCCCACGGCT
    TACGTCCGGC CCATGGACGG TCAAGATCAG GCTCCTAGTG AGTCACCTGA ACTGAAACCA
    CTGCCGGAGG ACTACCGGCA ACAGACCTTT GAAAAAACAG ACTTAAAAGT GCCTGCCAAA
    GCCAAGCTCA CCAAACTGAA GATGCCATCT CAGTCGGGTG AGCAGACCTA CTCCAATGAA
    GTTCATTGTG TTGAAGAGAT TTTGAAGGAA ATGACCCATT CATGGCCACC TCCTTTGACA
    GCAATACACA CGCCTAGTAC TGCTGAGCCC TCCAAATTTC CTTTTCCCAC AAAGGATTCT
    CAGCACATTA GTTCTGTGAC CCAAAATCAA AAACAATATG ATACATCTTC AAAAACCCAC
    ACTAATTCTC AGCAGGGAAC ATCCATGCTT GAAGATGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA ACGAACAAAG CCCAGAGAAG CCTCCTCCTG CGTCTGCGCC TCCCAGGTAT
    TGTGTGCATG TTGCCCCGCA GTCGCTTCCA GAAGCAGCGG CCTCAGCACA TTCCAGTAGT
    GCCGAGTCAG AGAGCACCAG TGACTCAGAC AGCTCCTCGG ACTCCGAGAG CGAGAGCAGC
    TCAAGTGACA GCGAAGAGAA TGAGCCCCTA GCAGCTCCGA CTCCTGAGCC TGAGCCTCCT
    ACAACAAACA AATGGCAGCT GGACAACTGG CTGACCAAAG TCAGCCAACC GGCAGCCCCA
    CCAGACACCC AGGAGGGGAG CGCAGAGCCC CCGCCTCGGC ACCCAGACAG TAAGGGCAAG
    GGGGGAGGCG ACAGCAGCGG CCCCACCACT GGTCACGAGC TCTCGGAATC CAAAGATCCT
    CCCCTCAAGA GCTCCGGCAA AGCCCCCCGG GGCTCCTCCG AGGGCCCTCA CCCCGGCAGG
    AGGAGCTGTC CGAAGTCCCC GGCCCAACAG GAGGCCCTGC CGAGGCAGAC TGTGGGAACC
    AAACAACCCA AGAAGCCGGG CAAGGCCTCC ACGCCGGCGG ACCCTCGGGC CGGCCTGCAG
    GTGGAGAGCG AGCCGGGCCC CCCTCCGTCC GGCTCCAGAG ACCAGCCTTC CAAAGACAAG
    CCCAAGGTGA AGACGAAAGG GAGGCCGCGC GCCGGCGACA GCAGGGAGCC CAAGCCCGCC
    GCGCCCAGCC CCGGTGACCG CAAGAAGCAC CAGAGCGGGC CGCTGGCCCC CTCAAAGGCA
    CTCTCGGGCC CCGAGCCCAC GAAGGACAAT GCGGGGGCCA GGAGCCCCGA GCACTTTGCC
    CTGGTTCCCC TGACCCAGAG CCAGGGCCCG CCCCACGGCG GTGGCGGCAG GACTAGTGGC
    TGCCGGCGCG CAGTGGTGGT CCAGGAGGAC CGCAGGAAGG ACAAGCTCCC GGGGCCTGCC
    AGAGACACCA AGCTGCTGTC GCCGCTCAGG GACCCTCCTC CCCCACCCAT CTTGATGGTG
    AAGATAACCC TGGACCTTCT TTCTCGGATA CCCCAGCTTC CTGGGAAAGG GGGCCGGCAG
    AAGAAGCCAG AGGACAAGCA GCCCCCCACA GGGAAGAAGC CCGATTTGGA GAAGAGAGGC
    GCAGACAACT CCAGCAAGTT GGTCAAAAAA AGAAAGGCTG AAGCTGAGAA AGACCATGAC
    AGCAAGAAAG CCAGACTGGA GAAAGAAACC AAGTTGCAGT CATTATCTTC ATCCCCCCAC
    AAAGAATCTT CCAAAACAAA AACACCCAGG CCCTCCTCTG AGCCCTCAAA GAAGGAGATG
    CTTCCCCCCC CACCTGTGTC ATCCTCATCC TCATCCTCCC AGAAGCCAGC CAAGCCTGCA
    CAAAAGAGGC CAAGGCGGGA AGCTGATCCC TCCAGCCAAG ACGCTTCCCA AAGTGCCAGT
    AGTAGCAAGG GCAACCACAA AGACTCTTCC ACTCCCAAGC ACAGAAAAGT AGAGGGGAAG
    GGATCTGGAA GCAGTACAGA GCACAAGGGA TCTTCTGGAG ATAGTGCATA TCCTTTTCTA
    GTGCCTTCTT TGCCAAATGG TAACTCTAAA CCAGGGAAGC CTCCAGTGAA GCTTGACAGA
    CAACAAGCAG ATTTTCACAG GAAGGAGGCC AAAAAGTTGA AGCAAAAAGC AGAGTTAATG
    ATGGACAAGG TTGGGAAGGC TTTTAAGTAC TTGGAGGCCG CCTTGTCCTT AATTGAGTGT
    GGGATTGCCA TGGAGTCGGA AAGCCCAGCC TCCAAATCGG CTTACTCGGT GTACTCCGAA
    ACCGTAGACC TCATTAAATT TATAATGTCA TTAAAATCCT TCTCAGACGC CTCATCTCCA
    CAAGAGAAAA TATTTGCTGT TTTATGCATG CGTTGTCAGT CCATTTTGAA CATGGCGATG
    TTTCGTTGTA AAAAAGATAT AGCAATAAAG TATTCTCGTG CTCTTAACGA ACACTTCAGC
    AGTTTTTTCA AAGTTGCCCA GGCACCTTCT CCATGCATTG CAAGAAACAC AGGCACACCG
    TCCCCTCTCT CTCCAGTTCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC CAGTGCTGGC
    AGCATAGGGA GCAGTGGTAT GCCTGTCACC ATCCAAAACA TGACATCTTC TTATGTCACC
    ATTACTTCCC ATGTCCTTAC CGCCTTTGAC CTCTGGGAAC AGGCTGAGGT CCTTACAAGG
    AAGAATAAAG AATTTTTTGC TCAGCTCAGC ACAAGTGTGT GTGCCTTGGC CCTCAACAGC
    AGTTTGATGG ATCTGGTGCA CTATACAAGA CAGGGTTTTC AGCGGCTAAA ACAAGCAACC
    AAAACACCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29733
    Clone ID Related Accession (Same CDS sequence) XM_022413582.1 , XM_014109957.1 , XM_014109958.1
    Accession Version XM_022413582.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AF4/FMR2 family member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CTGAAAGAGT CCAGAGCAGC AGTAGCAGCA GCTTGTACAA TGAAGACCGG 
    AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGAGAAAGAG
    GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCCTACA AGACAGAAAA AGGTGATGAG
    CTATCCAGTC GAATACAGAA CATGTTGGGA AACTATGAAG AAGTGAAGGA GTTCTTTAGC
    AGCAAGTCCC ACCCTCATCG CTTGGATGCC TCTGAAAATA GGTTGGGAAA GCCAAGATAT
    CCTTTATTTC CTGAAAAGGG GAGCAGCATT CCATCCCACT CCTTCCACAC GAGTGCCCAC
    CACCAGCCTG TTAATACTCC TGCCTCCGGA CCACTTCCTG TTGGTAACGT AAGCCAGAAC
    CCAAAGATGG CACAGCCAAG AATGGAACCA ATGTCAAATC CTCATGTCAA AAGCTATGGC
    CCACCAGACA GCCAGCACCC AACCCAAGAT CGCCTTGGTC AGGAGGTGTG CGGCTCTGGT
    CATCACAAAA AAGGTGACCG CAGAGCTGAT GGAGACCGCT GTGCTTTGGT GACAGACTCT
    GCTCCTGAGA GGGAGCTTTC TCCCTTACTC TCCTTGCCTT CCCCAGTGCC CCCCCTGTCA
    TCTGTACATT CCAACCAGCA AGCTCTTCCC CGGACACAAG GAAGCAGCAA GATTCACAGC
    AGTAACAGTA ATAGTAAAGG CTATTGCCCA GCCAAATCTC CCAAAGACCT AGTAGCAAAG
    GTCCATGATA AAGAGACCCC TCAAGACAAT TTAGTGGCAG TTGCCGGCCT TGGGGTGGCC
    CCTCCCCAGC CACCTTCTCA GACTTTCCCC CCACCTTCCC TCCCCTCAAA AACTGTTGCG
    ATGCAGCAGA AGCCCACGGC TTACGTCCGG CCCATGGACG GTCAAGATCA GGCTCCTAGT
    GAGTCACCTG AACTGAAACC ACTGCCGGAG GACTACCGGC AACAGACCTT TGAAAAAACA
    GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACCAAACTGA AGATGCCATC TCAGTCGGGT
    GAGCAGACCT ACTCCAATGA AGTTCATTGT GTTGAAGAGA TTTTGAAGGA AATGACCCAT
    TCATGGCCAC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CTGCTGAGCC CTCCAAATTT
    CCTTTTCCCA CAAAGGATTC TCAGCACATT AGTTCTGTGA CCCAAAATCA AAAACAATAT
    GATACATCTT CAAAAACCCA CACTAATTCT CAGCAGGGAA CATCCATGCT TGAAGATGAC
    CTTCAGCTCA GTGACAGTGA GGACAGTGAC AACGAACAAA GCCCAGAGAA GCCTCCTCCT
    GCGTCTGCGC CTCCCAGTGC CCCGCAGTCG CTTCCAGAAG CAGCGGCCTC AGCACATTCC
    AGTAGTGCCG AGTCAGAGAG CACCAGTGAC TCAGACAGCT CCTCGGACTC CGAGAGCGAG
    AGCAGCTCAA GTGACAGCGA AGAGAATGAG CCCCTAGCAG CTCCGACTCC TGAGCCTGAG
    CCTCCTACAA CAAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAACCGGCA
    GCCCCACCAG ACACCCAGGA GGGGAGCGCA GAGCCCCCGC CTCGGCACCC AGACAGTAAG
    GGCAAGGGGG GAGGCGACAG CAGCGGCCCC ACCACTGGTC ACGAGCTCTC GGAATCCAAA
    GATCCTCCCC TCAAGAGCTC CGGCAAAGCC CCCCGGGGCT CCTCCGAGGG CCCTCACCCC
    GGCAGGAGGA GCTGTCCGAA GTCCCCGGCC CAACAGGAGG CCCTGCCGAG GCAGACTGTG
    GGAACCAAAC AACCCAAGAA GCCGGGCAAG GCCTCCACGC CGGCGGACCC TCGGGCCGGC
    CTGCAGGTGG AGAGCGAGCC GGGCCCCCCT CCGTCCGGCT CCAGAGACCA GCCTTCCAAA
    GACAAGCCCA AGGTGAAGAC GAAAGGGAGG CCGCGCGCCG GCGACAGCAG GGAGCCCAAG
    CCCGCCGCGC CCAGCCCCGG TGACCGCAAG AAGCACCAGA GCGGGCCGCT GGCCCCCTCA
    AAGGCACTCT CGGGCCCCGA GCCCACGAAG GACAATGCGG GGGCCAGGAG CCCCGAGCAC
    TTTGCCCTGG TTCCCCTGAC CCAGAGCCAG GGCCCGCCCC ACGGCGGTGG CGGCAGGACT
    AGTGGCTGCC GGCGCGCAGT GGTGGTCCAG GAGGACCGCA GGAAGGACAA GCTCCCGGGG
    CCTGCCAGAG ACACCAAGCT GCTGTCGCCG CTCAGGGACC CTCCTCCCCC ACCCATCTTG
    ATGGTGAAGA TAACCCTGGA CCTTCTTTCT CGGATACCCC AGCTTCCTGG GAAAGGGGGC
    CGGCAGAAGA AGCCAGAGGA CAAGCAGCCC CCCACAGGGA AGAAGCCCGA TTTGGAGAAG
    AGAGGCGCAG ACAACTCCAG CAAGTTGGTC AAAAAAAGAA AGGCTGAAGC TGAGAAAGAC
    CATGACAGCA AGAAAGCCAG ACTGGAGAAA GAAACCAAGT TGCAGTCATT ATCTTCATCC
    CCCCACAAAG AATCTTCCAA AACAAAAACA CCCAGGCCCT CCTCTGAGCC CTCAAAGAAG
    GAGATGCTTC CCCCCCCACC TGTGTCATCC TCATCCTCAT CCTCCCAGAA GCCAGCCAAG
    CCTGCACAAA AGAGGCCAAG GCGGGAAGCT GATCCCTCCA GCCAAGACGC TTCCCAAAGT
    GCCAGTAGTA GCAAGGGCAA CCACAAAGAC TCTTCCACTC CCAAGCACAG AAAAGTAGAG
    GGGAAGGGAT CTGGAAGCAG TACAGAGCAC AAGGGATCTT CTGGAGATAG TGCATATCCT
    TTTCTAGTGC CTTCTTTGCC AAATGGTAAC TCTAAACCAG GGAAGCCTCC AGTGAAGCTT
    GACAGACAAC AAGCAGATTT TCACAGGAAG GAGGCCAAAA AGTTGAAGCA AAAAGCAGAG
    TTAATGATGG ACAAGGTTGG GAAGGCTTTT AAGTACTTGG AGGCCGCCTT GTCCTTAATT
    GAGTGTGGGA TTGCCATGGA GTCGGAAAGC CCAGCCTCCA AATCGGCTTA CTCGGTGTAC
    TCCGAAACCG TAGACCTCAT TAAATTTATA ATGTCATTAA AATCCTTCTC AGACGCCTCA
    TCTCCACAAG AGAAAATATT TGCTGTTTTA TGCATGCGTT GTCAGTCCAT TTTGAACATG
    GCGATGTTTC GTTGTAAAAA AGATATAGCA ATAAAGTATT CTCGTGCTCT TAACGAACAC
    TTCAGCAGTT TTTTCAAAGT TGCCCAGGCA CCTTCTCCAT GCATTGCAAG AAACACAGGC
    ACACCGTCCC CTCTCTCTCC AGTTCCTTCT CCTGCCAGCT CCGTAGGGTC CCAGTCCAGT
    GCTGGCAGCA TAGGGAGCAG TGGTATGCCT GTCACCATCC AAAACATGAC ATCTTCTTAT
    GTCACCATTA CTTCCCATGT CCTTACCGCC TTTGACCTCT GGGAACAGGC TGAGGTCCTT
    ACAAGGAAGA ATAAAGAATT TTTTGCTCAG CTCAGCACAA GTGTGTGTGC CTTGGCCCTC
    AACAGCAGTT TGATGGATCT GGTGCACTAT ACAAGACAGG GTTTTCAGCG GCTAAAACAA
    GCAACCAAAA CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269290.1
    CDS278..3955
    Translation

    Target ORF information:

    RefSeq Version XM_022413582.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413582.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CTGAAAGAGT CCAGAGCAGC AGTAGCAGCA GCTTGTACAA TGAAGACCGG 
    AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGAGAAAGAG
    GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCCTACA AGACAGAAAA AGGTGATGAG
    CTATCCAGTC GAATACAGAA CATGTTGGGA AACTATGAAG AAGTGAAGGA GTTCTTTAGC
    AGCAAGTCCC ACCCTCATCG CTTGGATGCC TCTGAAAATA GGTTGGGAAA GCCAAGATAT
    CCTTTATTTC CTGAAAAGGG GAGCAGCATT CCATCCCACT CCTTCCACAC GAGTGCCCAC
    CACCAGCCTG TTAATACTCC TGCCTCCGGA CCACTTCCTG TTGGTAACGT AAGCCAGAAC
    CCAAAGATGG CACAGCCAAG AATGGAACCA ATGTCAAATC CTCATGTCAA AAGCTATGGC
    CCACCAGACA GCCAGCACCC AACCCAAGAT CGCCTTGGTC AGGAGGTGTG CGGCTCTGGT
    CATCACAAAA AAGGTGACCG CAGAGCTGAT GGAGACCGCT GTGCTTTGGT GACAGACTCT
    GCTCCTGAGA GGGAGCTTTC TCCCTTACTC TCCTTGCCTT CCCCAGTGCC CCCCCTGTCA
    TCTGTACATT CCAACCAGCA AGCTCTTCCC CGGACACAAG GAAGCAGCAA GATTCACAGC
    AGTAACAGTA ATAGTAAAGG CTATTGCCCA GCCAAATCTC CCAAAGACCT AGTAGCAAAG
    GTCCATGATA AAGAGACCCC TCAAGACAAT TTAGTGGCAG TTGCCGGCCT TGGGGTGGCC
    CCTCCCCAGC CACCTTCTCA GACTTTCCCC CCACCTTCCC TCCCCTCAAA AACTGTTGCG
    ATGCAGCAGA AGCCCACGGC TTACGTCCGG CCCATGGACG GTCAAGATCA GGCTCCTAGT
    GAGTCACCTG AACTGAAACC ACTGCCGGAG GACTACCGGC AACAGACCTT TGAAAAAACA
    GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACCAAACTGA AGATGCCATC TCAGTCGGGT
    GAGCAGACCT ACTCCAATGA AGTTCATTGT GTTGAAGAGA TTTTGAAGGA AATGACCCAT
    TCATGGCCAC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CTGCTGAGCC CTCCAAATTT
    CCTTTTCCCA CAAAGGATTC TCAGCACATT AGTTCTGTGA CCCAAAATCA AAAACAATAT
    GATACATCTT CAAAAACCCA CACTAATTCT CAGCAGGGAA CATCCATGCT TGAAGATGAC
    CTTCAGCTCA GTGACAGTGA GGACAGTGAC AACGAACAAA GCCCAGAGAA GCCTCCTCCT
    GCGTCTGCGC CTCCCAGTGC CCCGCAGTCG CTTCCAGAAG CAGCGGCCTC AGCACATTCC
    AGTAGTGCCG AGTCAGAGAG CACCAGTGAC TCAGACAGCT CCTCGGACTC CGAGAGCGAG
    AGCAGCTCAA GTGACAGCGA AGAGAATGAG CCCCTAGCAG CTCCGACTCC TGAGCCTGAG
    CCTCCTACAA CAAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAACCGGCA
    GCCCCACCAG ACACCCAGGA GGGGAGCGCA GAGCCCCCGC CTCGGCACCC AGACAGTAAG
    GGCAAGGGGG GAGGCGACAG CAGCGGCCCC ACCACTGGTC ACGAGCTCTC GGAATCCAAA
    GATCCTCCCC TCAAGAGCTC CGGCAAAGCC CCCCGGGGCT CCTCCGAGGG CCCTCACCCC
    GGCAGGAGGA GCTGTCCGAA GTCCCCGGCC CAACAGGAGG CCCTGCCGAG GCAGACTGTG
    GGAACCAAAC AACCCAAGAA GCCGGGCAAG GCCTCCACGC CGGCGGACCC TCGGGCCGGC
    CTGCAGGTGG AGAGCGAGCC GGGCCCCCCT CCGTCCGGCT CCAGAGACCA GCCTTCCAAA
    GACAAGCCCA AGGTGAAGAC GAAAGGGAGG CCGCGCGCCG GCGACAGCAG GGAGCCCAAG
    CCCGCCGCGC CCAGCCCCGG TGACCGCAAG AAGCACCAGA GCGGGCCGCT GGCCCCCTCA
    AAGGCACTCT CGGGCCCCGA GCCCACGAAG GACAATGCGG GGGCCAGGAG CCCCGAGCAC
    TTTGCCCTGG TTCCCCTGAC CCAGAGCCAG GGCCCGCCCC ACGGCGGTGG CGGCAGGACT
    AGTGGCTGCC GGCGCGCAGT GGTGGTCCAG GAGGACCGCA GGAAGGACAA GCTCCCGGGG
    CCTGCCAGAG ACACCAAGCT GCTGTCGCCG CTCAGGGACC CTCCTCCCCC ACCCATCTTG
    ATGGTGAAGA TAACCCTGGA CCTTCTTTCT CGGATACCCC AGCTTCCTGG GAAAGGGGGC
    CGGCAGAAGA AGCCAGAGGA CAAGCAGCCC CCCACAGGGA AGAAGCCCGA TTTGGAGAAG
    AGAGGCGCAG ACAACTCCAG CAAGTTGGTC AAAAAAAGAA AGGCTGAAGC TGAGAAAGAC
    CATGACAGCA AGAAAGCCAG ACTGGAGAAA GAAACCAAGT TGCAGTCATT ATCTTCATCC
    CCCCACAAAG AATCTTCCAA AACAAAAACA CCCAGGCCCT CCTCTGAGCC CTCAAAGAAG
    GAGATGCTTC CCCCCCCACC TGTGTCATCC TCATCCTCAT CCTCCCAGAA GCCAGCCAAG
    CCTGCACAAA AGAGGCCAAG GCGGGAAGCT GATCCCTCCA GCCAAGACGC TTCCCAAAGT
    GCCAGTAGTA GCAAGGGCAA CCACAAAGAC TCTTCCACTC CCAAGCACAG AAAAGTAGAG
    GGGAAGGGAT CTGGAAGCAG TACAGAGCAC AAGGGATCTT CTGGAGATAG TGCATATCCT
    TTTCTAGTGC CTTCTTTGCC AAATGGTAAC TCTAAACCAG GGAAGCCTCC AGTGAAGCTT
    GACAGACAAC AAGCAGATTT TCACAGGAAG GAGGCCAAAA AGTTGAAGCA AAAAGCAGAG
    TTAATGATGG ACAAGGTTGG GAAGGCTTTT AAGTACTTGG AGGCCGCCTT GTCCTTAATT
    GAGTGTGGGA TTGCCATGGA GTCGGAAAGC CCAGCCTCCA AATCGGCTTA CTCGGTGTAC
    TCCGAAACCG TAGACCTCAT TAAATTTATA ATGTCATTAA AATCCTTCTC AGACGCCTCA
    TCTCCACAAG AGAAAATATT TGCTGTTTTA TGCATGCGTT GTCAGTCCAT TTTGAACATG
    GCGATGTTTC GTTGTAAAAA AGATATAGCA ATAAAGTATT CTCGTGCTCT TAACGAACAC
    TTCAGCAGTT TTTTCAAAGT TGCCCAGGCA CCTTCTCCAT GCATTGCAAG AAACACAGGC
    ACACCGTCCC CTCTCTCTCC AGTTCCTTCT CCTGCCAGCT CCGTAGGGTC CCAGTCCAGT
    GCTGGCAGCA TAGGGAGCAG TGGTATGCCT GTCACCATCC AAAACATGAC ATCTTCTTAT
    GTCACCATTA CTTCCCATGT CCTTACCGCC TTTGACCTCT GGGAACAGGC TGAGGTCCTT
    ACAAGGAAGA ATAAAGAATT TTTTGCTCAG CTCAGCACAA GTGTGTGTGC CTTGGCCCTC
    AACAGCAGTT TGATGGATCT GGTGCACTAT ACAAGACAGG GTTTTCAGCG GCTAAAACAA
    GCAACCAAAA CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29733
    Clone ID Related Accession (Same CDS sequence) XM_022413582.1 , XM_014109957.1 , XM_014109958.1
    Accession Version XM_014109957.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product AF4/FMR2 family member 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CTGAAAGAGT CCAGAGCAGC AGTAGCAGCA GCTTGTACAA TGAAGACCGG 
    AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGAGAAAGAG
    GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCCTACA AGACAGAAAA AGGTGATGAG
    CTATCCAGTC GAATACAGAA CATGTTGGGA AACTATGAAG AAGTGAAGGA GTTCTTTAGC
    AGCAAGTCCC ACCCTCATCG CTTGGATGCC TCTGAAAATA GGTTGGGAAA GCCAAGATAT
    CCTTTATTTC CTGAAAAGGG GAGCAGCATT CCATCCCACT CCTTCCACAC GAGTGCCCAC
    CACCAGCCTG TTAATACTCC TGCCTCCGGA CCACTTCCTG TTGGTAACGT AAGCCAGAAC
    CCAAAGATGG CACAGCCAAG AATGGAACCA ATGTCAAATC CTCATGTCAA AAGCTATGGC
    CCACCAGACA GCCAGCACCC AACCCAAGAT CGCCTTGGTC AGGAGGTGTG CGGCTCTGGT
    CATCACAAAA AAGGTGACCG CAGAGCTGAT GGAGACCGCT GTGCTTTGGT GACAGACTCT
    GCTCCTGAGA GGGAGCTTTC TCCCTTACTC TCCTTGCCTT CCCCAGTGCC CCCCCTGTCA
    TCTGTACATT CCAACCAGCA AGCTCTTCCC CGGACACAAG GAAGCAGCAA GATTCACAGC
    AGTAACAGTA ATAGTAAAGG CTATTGCCCA GCCAAATCTC CCAAAGACCT AGTAGCAAAG
    GTCCATGATA AAGAGACCCC TCAAGACAAT TTAGTGGCAG TTGCCGGCCT TGGGGTGGCC
    CCTCCCCAGC CACCTTCTCA GACTTTCCCC CCACCTTCCC TCCCCTCAAA AACTGTTGCG
    ATGCAGCAGA AGCCCACGGC TTACGTCCGG CCCATGGACG GTCAAGATCA GGCTCCTAGT
    GAGTCACCTG AACTGAAACC ACTGCCGGAG GACTACCGGC AACAGACCTT TGAAAAAACA
    GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACCAAACTGA AGATGCCATC TCAGTCGGGT
    GAGCAGACCT ACTCCAATGA AGTTCATTGT GTTGAAGAGA TTTTGAAGGA AATGACCCAT
    TCATGGCCAC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CTGCTGAGCC CTCCAAATTT
    CCTTTTCCCA CAAAGGATTC TCAGCACATT AGTTCTGTGA CCCAAAATCA AAAACAATAT
    GATACATCTT CAAAAACCCA CACTAATTCT CAGCAGGGAA CATCCATGCT TGAAGATGAC
    CTTCAGCTCA GTGACAGTGA GGACAGTGAC AACGAACAAA GCCCAGAGAA GCCTCCTCCT
    GCGTCTGCGC CTCCCAGTGC CCCGCAGTCG CTTCCAGAAG CAGCGGCCTC AGCACATTCC
    AGTAGTGCCG AGTCAGAGAG CACCAGTGAC TCAGACAGCT CCTCGGACTC CGAGAGCGAG
    AGCAGCTCAA GTGACAGCGA AGAGAATGAG CCCCTAGCAG CTCCGACTCC TGAGCCTGAG
    CCTCCTACAA CAAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAACCGGCA
    GCCCCACCAG ACACCCAGGA GGGGAGCGCA GAGCCCCCGC CTCGGCACCC AGACAGTAAG
    GGCAAGGGGG GAGGCGACAG CAGCGGCCCC ACCACTGGTC ACGAGCTCTC GGAATCCAAA
    GATCCTCCCC TCAAGAGCTC CGGCAAAGCC CCCCGGGGCT CCTCCGAGGG CCCTCACCCC
    GGCAGGAGGA GCTGTCCGAA GTCCCCGGCC CAACAGGAGG CCCTGCCGAG GCAGACTGTG
    GGAACCAAAC AACCCAAGAA GCCGGGCAAG GCCTCCACGC CGGCGGACCC TCGGGCCGGC
    CTGCAGGTGG AGAGCGAGCC GGGCCCCCCT CCGTCCGGCT CCAGAGACCA GCCTTCCAAA
    GACAAGCCCA AGGTGAAGAC GAAAGGGAGG CCGCGCGCCG GCGACAGCAG GGAGCCCAAG
    CCCGCCGCGC CCAGCCCCGG TGACCGCAAG AAGCACCAGA GCGGGCCGCT GGCCCCCTCA
    AAGGCACTCT CGGGCCCCGA GCCCACGAAG GACAATGCGG GGGCCAGGAG CCCCGAGCAC
    TTTGCCCTGG TTCCCCTGAC CCAGAGCCAG GGCCCGCCCC ACGGCGGTGG CGGCAGGACT
    AGTGGCTGCC GGCGCGCAGT GGTGGTCCAG GAGGACCGCA GGAAGGACAA GCTCCCGGGG
    CCTGCCAGAG ACACCAAGCT GCTGTCGCCG CTCAGGGACC CTCCTCCCCC ACCCATCTTG
    ATGGTGAAGA TAACCCTGGA CCTTCTTTCT CGGATACCCC AGCTTCCTGG GAAAGGGGGC
    CGGCAGAAGA AGCCAGAGGA CAAGCAGCCC CCCACAGGGA AGAAGCCCGA TTTGGAGAAG
    AGAGGCGCAG ACAACTCCAG CAAGTTGGTC AAAAAAAGAA AGGCTGAAGC TGAGAAAGAC
    CATGACAGCA AGAAAGCCAG ACTGGAGAAA GAAACCAAGT TGCAGTCATT ATCTTCATCC
    CCCCACAAAG AATCTTCCAA AACAAAAACA CCCAGGCCCT CCTCTGAGCC CTCAAAGAAG
    GAGATGCTTC CCCCCCCACC TGTGTCATCC TCATCCTCAT CCTCCCAGAA GCCAGCCAAG
    CCTGCACAAA AGAGGCCAAG GCGGGAAGCT GATCCCTCCA GCCAAGACGC TTCCCAAAGT
    GCCAGTAGTA GCAAGGGCAA CCACAAAGAC TCTTCCACTC CCAAGCACAG AAAAGTAGAG
    GGGAAGGGAT CTGGAAGCAG TACAGAGCAC AAGGGATCTT CTGGAGATAG TGCATATCCT
    TTTCTAGTGC CTTCTTTGCC AAATGGTAAC TCTAAACCAG GGAAGCCTCC AGTGAAGCTT
    GACAGACAAC AAGCAGATTT TCACAGGAAG GAGGCCAAAA AGTTGAAGCA AAAAGCAGAG
    TTAATGATGG ACAAGGTTGG GAAGGCTTTT AAGTACTTGG AGGCCGCCTT GTCCTTAATT
    GAGTGTGGGA TTGCCATGGA GTCGGAAAGC CCAGCCTCCA AATCGGCTTA CTCGGTGTAC
    TCCGAAACCG TAGACCTCAT TAAATTTATA ATGTCATTAA AATCCTTCTC AGACGCCTCA
    TCTCCACAAG AGAAAATATT TGCTGTTTTA TGCATGCGTT GTCAGTCCAT TTTGAACATG
    GCGATGTTTC GTTGTAAAAA AGATATAGCA ATAAAGTATT CTCGTGCTCT TAACGAACAC
    TTCAGCAGTT TTTTCAAAGT TGCCCAGGCA CCTTCTCCAT GCATTGCAAG AAACACAGGC
    ACACCGTCCC CTCTCTCTCC AGTTCCTTCT CCTGCCAGCT CCGTAGGGTC CCAGTCCAGT
    GCTGGCAGCA TAGGGAGCAG TGGTATGCCT GTCACCATCC AAAACATGAC ATCTTCTTAT
    GTCACCATTA CTTCCCATGT CCTTACCGCC TTTGACCTCT GGGAACAGGC TGAGGTCCTT
    ACAAGGAAGA ATAAAGAATT TTTTGCTCAG CTCAGCACAA GTGTGTGTGC CTTGGCCCTC
    AACAGCAGTT TGATGGATCT GGTGCACTAT ACAAGACAGG GTTTTCAGCG GCTAAAACAA
    GCAACCAAAA CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965432.1
    CDS316..3993
    Translation

    Target ORF information:

    RefSeq Version XM_014109957.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris AF4/FMR2 family, member 1 (AFF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109957.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CTGAAAGAGT CCAGAGCAGC AGTAGCAGCA GCTTGTACAA TGAAGACCGG 
    AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGAGAAAGAG
    GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCCTACA AGACAGAAAA AGGTGATGAG
    CTATCCAGTC GAATACAGAA CATGTTGGGA AACTATGAAG AAGTGAAGGA GTTCTTTAGC
    AGCAAGTCCC ACCCTCATCG CTTGGATGCC TCTGAAAATA GGTTGGGAAA GCCAAGATAT
    CCTTTATTTC CTGAAAAGGG GAGCAGCATT CCATCCCACT CCTTCCACAC GAGTGCCCAC
    CACCAGCCTG TTAATACTCC TGCCTCCGGA CCACTTCCTG TTGGTAACGT AAGCCAGAAC
    CCAAAGATGG CACAGCCAAG AATGGAACCA ATGTCAAATC CTCATGTCAA AAGCTATGGC
    CCACCAGACA GCCAGCACCC AACCCAAGAT CGCCTTGGTC AGGAGGTGTG CGGCTCTGGT
    CATCACAAAA AAGGTGACCG CAGAGCTGAT GGAGACCGCT GTGCTTTGGT GACAGACTCT
    GCTCCTGAGA GGGAGCTTTC TCCCTTACTC TCCTTGCCTT CCCCAGTGCC CCCCCTGTCA
    TCTGTACATT CCAACCAGCA AGCTCTTCCC CGGACACAAG GAAGCAGCAA GATTCACAGC
    AGTAACAGTA ATAGTAAAGG CTATTGCCCA GCCAAATCTC CCAAAGACCT AGTAGCAAAG
    GTCCATGATA AAGAGACCCC TCAAGACAAT TTAGTGGCAG TTGCCGGCCT TGGGGTGGCC
    CCTCCCCAGC CACCTTCTCA GACTTTCCCC CCACCTTCCC TCCCCTCAAA AACTGTTGCG
    ATGCAGCAGA AGCCCACGGC TTACGTCCGG CCCATGGACG GTCAAGATCA GGCTCCTAGT
    GAGTCACCTG AACTGAAACC ACTGCCGGAG GACTACCGGC AACAGACCTT TGAAAAAACA
    GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACCAAACTGA AGATGCCATC TCAGTCGGGT
    GAGCAGACCT ACTCCAATGA AGTTCATTGT GTTGAAGAGA TTTTGAAGGA AATGACCCAT
    TCATGGCCAC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CTGCTGAGCC CTCCAAATTT
    CCTTTTCCCA CAAAGGATTC TCAGCACATT AGTTCTGTGA CCCAAAATCA AAAACAATAT
    GATACATCTT CAAAAACCCA CACTAATTCT CAGCAGGGAA CATCCATGCT TGAAGATGAC
    CTTCAGCTCA GTGACAGTGA GGACAGTGAC AACGAACAAA GCCCAGAGAA GCCTCCTCCT
    GCGTCTGCGC CTCCCAGTGC CCCGCAGTCG CTTCCAGAAG CAGCGGCCTC AGCACATTCC
    AGTAGTGCCG AGTCAGAGAG CACCAGTGAC TCAGACAGCT CCTCGGACTC CGAGAGCGAG
    AGCAGCTCAA GTGACAGCGA AGAGAATGAG CCCCTAGCAG CTCCGACTCC TGAGCCTGAG
    CCTCCTACAA CAAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAACCGGCA
    GCCCCACCAG ACACCCAGGA GGGGAGCGCA GAGCCCCCGC CTCGGCACCC AGACAGTAAG
    GGCAAGGGGG GAGGCGACAG CAGCGGCCCC ACCACTGGTC ACGAGCTCTC GGAATCCAAA
    GATCCTCCCC TCAAGAGCTC CGGCAAAGCC CCCCGGGGCT CCTCCGAGGG CCCTCACCCC
    GGCAGGAGGA GCTGTCCGAA GTCCCCGGCC CAACAGGAGG CCCTGCCGAG GCAGACTGTG
    GGAACCAAAC AACCCAAGAA GCCGGGCAAG GCCTCCACGC CGGCGGACCC TCGGGCCGGC
    CTGCAGGTGG AGAGCGAGCC GGGCCCCCCT CCGTCCGGCT CCAGAGACCA GCCTTCCAAA
    GACAAGCCCA AGGTGAAGAC GAAAGGGAGG CCGCGCGCCG GCGACAGCAG GGAGCCCAAG
    CCCGCCGCGC CCAGCCCCGG TGACCGCAAG AAGCACCAGA GCGGGCCGCT GGCCCCCTCA
    AAGGCACTCT CGGGCCCCGA GCCCACGAAG GACAATGCGG GGGCCAGGAG CCCCGAGCAC
    TTTGCCCTGG TTCCCCTGAC CCAGAGCCAG GGCCCGCCCC ACGGCGGTGG CGGCAGGACT
    AGTGGCTGCC GGCGCGCAGT GGTGGTCCAG GAGGACCGCA GGAAGGACAA GCTCCCGGGG
    CCTGCCAGAG ACACCAAGCT GCTGTCGCCG CTCAGGGACC CTCCTCCCCC ACCCATCTTG
    ATGGTGAAGA TAACCCTGGA CCTTCTTTCT CGGATACCCC AGCTTCCTGG GAAAGGGGGC
    CGGCAGAAGA AGCCAGAGGA CAAGCAGCCC CCCACAGGGA AGAAGCCCGA TTTGGAGAAG
    AGAGGCGCAG ACAACTCCAG CAAGTTGGTC AAAAAAAGAA AGGCTGAAGC TGAGAAAGAC
    CATGACAGCA AGAAAGCCAG ACTGGAGAAA GAAACCAAGT TGCAGTCATT ATCTTCATCC
    CCCCACAAAG AATCTTCCAA AACAAAAACA CCCAGGCCCT CCTCTGAGCC CTCAAAGAAG
    GAGATGCTTC CCCCCCCACC TGTGTCATCC TCATCCTCAT CCTCCCAGAA GCCAGCCAAG
    CCTGCACAAA AGAGGCCAAG GCGGGAAGCT GATCCCTCCA GCCAAGACGC TTCCCAAAGT
    GCCAGTAGTA GCAAGGGCAA CCACAAAGAC TCTTCCACTC CCAAGCACAG AAAAGTAGAG
    GGGAAGGGAT CTGGAAGCAG TACAGAGCAC AAGGGATCTT CTGGAGATAG TGCATATCCT
    TTTCTAGTGC CTTCTTTGCC AAATGGTAAC TCTAAACCAG GGAAGCCTCC AGTGAAGCTT
    GACAGACAAC AAGCAGATTT TCACAGGAAG GAGGCCAAAA AGTTGAAGCA AAAAGCAGAG
    TTAATGATGG ACAAGGTTGG GAAGGCTTTT AAGTACTTGG AGGCCGCCTT GTCCTTAATT
    GAGTGTGGGA TTGCCATGGA GTCGGAAAGC CCAGCCTCCA AATCGGCTTA CTCGGTGTAC
    TCCGAAACCG TAGACCTCAT TAAATTTATA ATGTCATTAA AATCCTTCTC AGACGCCTCA
    TCTCCACAAG AGAAAATATT TGCTGTTTTA TGCATGCGTT GTCAGTCCAT TTTGAACATG
    GCGATGTTTC GTTGTAAAAA AGATATAGCA ATAAAGTATT CTCGTGCTCT TAACGAACAC
    TTCAGCAGTT TTTTCAAAGT TGCCCAGGCA CCTTCTCCAT GCATTGCAAG AAACACAGGC
    ACACCGTCCC CTCTCTCTCC AGTTCCTTCT CCTGCCAGCT CCGTAGGGTC CCAGTCCAGT
    GCTGGCAGCA TAGGGAGCAG TGGTATGCCT GTCACCATCC AAAACATGAC ATCTTCTTAT
    GTCACCATTA CTTCCCATGT CCTTACCGCC TTTGACCTCT GGGAACAGGC TGAGGTCCTT
    ACAAGGAAGA ATAAAGAATT TTTTGCTCAG CTCAGCACAA GTGTGTGTGC CTTGGCCCTC
    AACAGCAGTT TGATGGATCT GGTGCACTAT ACAAGACAGG GTTTTCAGCG GCTAAAACAA
    GCAACCAAAA CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29733
    Clone ID Related Accession (Same CDS sequence) XM_022413582.1 , XM_014109957.1 , XM_014109958.1
    Accession Version XM_014109958.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product AF4/FMR2 family member 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CTGAAAGAGT CCAGAGCAGC AGTAGCAGCA GCTTGTACAA TGAAGACCGG 
    AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGAGAAAGAG
    GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCCTACA AGACAGAAAA AGGTGATGAG
    CTATCCAGTC GAATACAGAA CATGTTGGGA AACTATGAAG AAGTGAAGGA GTTCTTTAGC
    AGCAAGTCCC ACCCTCATCG CTTGGATGCC TCTGAAAATA GGTTGGGAAA GCCAAGATAT
    CCTTTATTTC CTGAAAAGGG GAGCAGCATT CCATCCCACT CCTTCCACAC GAGTGCCCAC
    CACCAGCCTG TTAATACTCC TGCCTCCGGA CCACTTCCTG TTGGTAACGT AAGCCAGAAC
    CCAAAGATGG CACAGCCAAG AATGGAACCA ATGTCAAATC CTCATGTCAA AAGCTATGGC
    CCACCAGACA GCCAGCACCC AACCCAAGAT CGCCTTGGTC AGGAGGTGTG CGGCTCTGGT
    CATCACAAAA AAGGTGACCG CAGAGCTGAT GGAGACCGCT GTGCTTTGGT GACAGACTCT
    GCTCCTGAGA GGGAGCTTTC TCCCTTACTC TCCTTGCCTT CCCCAGTGCC CCCCCTGTCA
    TCTGTACATT CCAACCAGCA AGCTCTTCCC CGGACACAAG GAAGCAGCAA GATTCACAGC
    AGTAACAGTA ATAGTAAAGG CTATTGCCCA GCCAAATCTC CCAAAGACCT AGTAGCAAAG
    GTCCATGATA AAGAGACCCC TCAAGACAAT TTAGTGGCAG TTGCCGGCCT TGGGGTGGCC
    CCTCCCCAGC CACCTTCTCA GACTTTCCCC CCACCTTCCC TCCCCTCAAA AACTGTTGCG
    ATGCAGCAGA AGCCCACGGC TTACGTCCGG CCCATGGACG GTCAAGATCA GGCTCCTAGT
    GAGTCACCTG AACTGAAACC ACTGCCGGAG GACTACCGGC AACAGACCTT TGAAAAAACA
    GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACCAAACTGA AGATGCCATC TCAGTCGGGT
    GAGCAGACCT ACTCCAATGA AGTTCATTGT GTTGAAGAGA TTTTGAAGGA AATGACCCAT
    TCATGGCCAC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CTGCTGAGCC CTCCAAATTT
    CCTTTTCCCA CAAAGGATTC TCAGCACATT AGTTCTGTGA CCCAAAATCA AAAACAATAT
    GATACATCTT CAAAAACCCA CACTAATTCT CAGCAGGGAA CATCCATGCT TGAAGATGAC
    CTTCAGCTCA GTGACAGTGA GGACAGTGAC AACGAACAAA GCCCAGAGAA GCCTCCTCCT
    GCGTCTGCGC CTCCCAGTGC CCCGCAGTCG CTTCCAGAAG CAGCGGCCTC AGCACATTCC
    AGTAGTGCCG AGTCAGAGAG CACCAGTGAC TCAGACAGCT CCTCGGACTC CGAGAGCGAG
    AGCAGCTCAA GTGACAGCGA AGAGAATGAG CCCCTAGCAG CTCCGACTCC TGAGCCTGAG
    CCTCCTACAA CAAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAACCGGCA
    GCCCCACCAG ACACCCAGGA GGGGAGCGCA GAGCCCCCGC CTCGGCACCC AGACAGTAAG
    GGCAAGGGGG GAGGCGACAG CAGCGGCCCC ACCACTGGTC ACGAGCTCTC GGAATCCAAA
    GATCCTCCCC TCAAGAGCTC CGGCAAAGCC CCCCGGGGCT CCTCCGAGGG CCCTCACCCC
    GGCAGGAGGA GCTGTCCGAA GTCCCCGGCC CAACAGGAGG CCCTGCCGAG GCAGACTGTG
    GGAACCAAAC AACCCAAGAA GCCGGGCAAG GCCTCCACGC CGGCGGACCC TCGGGCCGGC
    CTGCAGGTGG AGAGCGAGCC GGGCCCCCCT CCGTCCGGCT CCAGAGACCA GCCTTCCAAA
    GACAAGCCCA AGGTGAAGAC GAAAGGGAGG CCGCGCGCCG GCGACAGCAG GGAGCCCAAG
    CCCGCCGCGC CCAGCCCCGG TGACCGCAAG AAGCACCAGA GCGGGCCGCT GGCCCCCTCA
    AAGGCACTCT CGGGCCCCGA GCCCACGAAG GACAATGCGG GGGCCAGGAG CCCCGAGCAC
    TTTGCCCTGG TTCCCCTGAC CCAGAGCCAG GGCCCGCCCC ACGGCGGTGG CGGCAGGACT
    AGTGGCTGCC GGCGCGCAGT GGTGGTCCAG GAGGACCGCA GGAAGGACAA GCTCCCGGGG
    CCTGCCAGAG ACACCAAGCT GCTGTCGCCG CTCAGGGACC CTCCTCCCCC ACCCATCTTG
    ATGGTGAAGA TAACCCTGGA CCTTCTTTCT CGGATACCCC AGCTTCCTGG GAAAGGGGGC
    CGGCAGAAGA AGCCAGAGGA CAAGCAGCCC CCCACAGGGA AGAAGCCCGA TTTGGAGAAG
    AGAGGCGCAG ACAACTCCAG CAAGTTGGTC AAAAAAAGAA AGGCTGAAGC TGAGAAAGAC
    CATGACAGCA AGAAAGCCAG ACTGGAGAAA GAAACCAAGT TGCAGTCATT ATCTTCATCC
    CCCCACAAAG AATCTTCCAA AACAAAAACA CCCAGGCCCT CCTCTGAGCC CTCAAAGAAG
    GAGATGCTTC CCCCCCCACC TGTGTCATCC TCATCCTCAT CCTCCCAGAA GCCAGCCAAG
    CCTGCACAAA AGAGGCCAAG GCGGGAAGCT GATCCCTCCA GCCAAGACGC TTCCCAAAGT
    GCCAGTAGTA GCAAGGGCAA CCACAAAGAC TCTTCCACTC CCAAGCACAG AAAAGTAGAG
    GGGAAGGGAT CTGGAAGCAG TACAGAGCAC AAGGGATCTT CTGGAGATAG TGCATATCCT
    TTTCTAGTGC CTTCTTTGCC AAATGGTAAC TCTAAACCAG GGAAGCCTCC AGTGAAGCTT
    GACAGACAAC AAGCAGATTT TCACAGGAAG GAGGCCAAAA AGTTGAAGCA AAAAGCAGAG
    TTAATGATGG ACAAGGTTGG GAAGGCTTTT AAGTACTTGG AGGCCGCCTT GTCCTTAATT
    GAGTGTGGGA TTGCCATGGA GTCGGAAAGC CCAGCCTCCA AATCGGCTTA CTCGGTGTAC
    TCCGAAACCG TAGACCTCAT TAAATTTATA ATGTCATTAA AATCCTTCTC AGACGCCTCA
    TCTCCACAAG AGAAAATATT TGCTGTTTTA TGCATGCGTT GTCAGTCCAT TTTGAACATG
    GCGATGTTTC GTTGTAAAAA AGATATAGCA ATAAAGTATT CTCGTGCTCT TAACGAACAC
    TTCAGCAGTT TTTTCAAAGT TGCCCAGGCA CCTTCTCCAT GCATTGCAAG AAACACAGGC
    ACACCGTCCC CTCTCTCTCC AGTTCCTTCT CCTGCCAGCT CCGTAGGGTC CCAGTCCAGT
    GCTGGCAGCA TAGGGAGCAG TGGTATGCCT GTCACCATCC AAAACATGAC ATCTTCTTAT
    GTCACCATTA CTTCCCATGT CCTTACCGCC TTTGACCTCT GGGAACAGGC TGAGGTCCTT
    ACAAGGAAGA ATAAAGAATT TTTTGCTCAG CTCAGCACAA GTGTGTGTGC CTTGGCCCTC
    AACAGCAGTT TGATGGATCT GGTGCACTAT ACAAGACAGG GTTTTCAGCG GCTAAAACAA
    GCAACCAAAA CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965433.1
    CDS316..3993
    Translation

    Target ORF information:

    RefSeq Version XM_014109958.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris AF4/FMR2 family, member 1 (AFF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109958.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CTGAAAGAGT CCAGAGCAGC AGTAGCAGCA GCTTGTACAA TGAAGACCGG 
    AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGAGAAAGAG
    GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCCTACA AGACAGAAAA AGGTGATGAG
    CTATCCAGTC GAATACAGAA CATGTTGGGA AACTATGAAG AAGTGAAGGA GTTCTTTAGC
    AGCAAGTCCC ACCCTCATCG CTTGGATGCC TCTGAAAATA GGTTGGGAAA GCCAAGATAT
    CCTTTATTTC CTGAAAAGGG GAGCAGCATT CCATCCCACT CCTTCCACAC GAGTGCCCAC
    CACCAGCCTG TTAATACTCC TGCCTCCGGA CCACTTCCTG TTGGTAACGT AAGCCAGAAC
    CCAAAGATGG CACAGCCAAG AATGGAACCA ATGTCAAATC CTCATGTCAA AAGCTATGGC
    CCACCAGACA GCCAGCACCC AACCCAAGAT CGCCTTGGTC AGGAGGTGTG CGGCTCTGGT
    CATCACAAAA AAGGTGACCG CAGAGCTGAT GGAGACCGCT GTGCTTTGGT GACAGACTCT
    GCTCCTGAGA GGGAGCTTTC TCCCTTACTC TCCTTGCCTT CCCCAGTGCC CCCCCTGTCA
    TCTGTACATT CCAACCAGCA AGCTCTTCCC CGGACACAAG GAAGCAGCAA GATTCACAGC
    AGTAACAGTA ATAGTAAAGG CTATTGCCCA GCCAAATCTC CCAAAGACCT AGTAGCAAAG
    GTCCATGATA AAGAGACCCC TCAAGACAAT TTAGTGGCAG TTGCCGGCCT TGGGGTGGCC
    CCTCCCCAGC CACCTTCTCA GACTTTCCCC CCACCTTCCC TCCCCTCAAA AACTGTTGCG
    ATGCAGCAGA AGCCCACGGC TTACGTCCGG CCCATGGACG GTCAAGATCA GGCTCCTAGT
    GAGTCACCTG AACTGAAACC ACTGCCGGAG GACTACCGGC AACAGACCTT TGAAAAAACA
    GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACCAAACTGA AGATGCCATC TCAGTCGGGT
    GAGCAGACCT ACTCCAATGA AGTTCATTGT GTTGAAGAGA TTTTGAAGGA AATGACCCAT
    TCATGGCCAC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CTGCTGAGCC CTCCAAATTT
    CCTTTTCCCA CAAAGGATTC TCAGCACATT AGTTCTGTGA CCCAAAATCA AAAACAATAT
    GATACATCTT CAAAAACCCA CACTAATTCT CAGCAGGGAA CATCCATGCT TGAAGATGAC
    CTTCAGCTCA GTGACAGTGA GGACAGTGAC AACGAACAAA GCCCAGAGAA GCCTCCTCCT
    GCGTCTGCGC CTCCCAGTGC CCCGCAGTCG CTTCCAGAAG CAGCGGCCTC AGCACATTCC
    AGTAGTGCCG AGTCAGAGAG CACCAGTGAC TCAGACAGCT CCTCGGACTC CGAGAGCGAG
    AGCAGCTCAA GTGACAGCGA AGAGAATGAG CCCCTAGCAG CTCCGACTCC TGAGCCTGAG
    CCTCCTACAA CAAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAACCGGCA
    GCCCCACCAG ACACCCAGGA GGGGAGCGCA GAGCCCCCGC CTCGGCACCC AGACAGTAAG
    GGCAAGGGGG GAGGCGACAG CAGCGGCCCC ACCACTGGTC ACGAGCTCTC GGAATCCAAA
    GATCCTCCCC TCAAGAGCTC CGGCAAAGCC CCCCGGGGCT CCTCCGAGGG CCCTCACCCC
    GGCAGGAGGA GCTGTCCGAA GTCCCCGGCC CAACAGGAGG CCCTGCCGAG GCAGACTGTG
    GGAACCAAAC AACCCAAGAA GCCGGGCAAG GCCTCCACGC CGGCGGACCC TCGGGCCGGC
    CTGCAGGTGG AGAGCGAGCC GGGCCCCCCT CCGTCCGGCT CCAGAGACCA GCCTTCCAAA
    GACAAGCCCA AGGTGAAGAC GAAAGGGAGG CCGCGCGCCG GCGACAGCAG GGAGCCCAAG
    CCCGCCGCGC CCAGCCCCGG TGACCGCAAG AAGCACCAGA GCGGGCCGCT GGCCCCCTCA
    AAGGCACTCT CGGGCCCCGA GCCCACGAAG GACAATGCGG GGGCCAGGAG CCCCGAGCAC
    TTTGCCCTGG TTCCCCTGAC CCAGAGCCAG GGCCCGCCCC ACGGCGGTGG CGGCAGGACT
    AGTGGCTGCC GGCGCGCAGT GGTGGTCCAG GAGGACCGCA GGAAGGACAA GCTCCCGGGG
    CCTGCCAGAG ACACCAAGCT GCTGTCGCCG CTCAGGGACC CTCCTCCCCC ACCCATCTTG
    ATGGTGAAGA TAACCCTGGA CCTTCTTTCT CGGATACCCC AGCTTCCTGG GAAAGGGGGC
    CGGCAGAAGA AGCCAGAGGA CAAGCAGCCC CCCACAGGGA AGAAGCCCGA TTTGGAGAAG
    AGAGGCGCAG ACAACTCCAG CAAGTTGGTC AAAAAAAGAA AGGCTGAAGC TGAGAAAGAC
    CATGACAGCA AGAAAGCCAG ACTGGAGAAA GAAACCAAGT TGCAGTCATT ATCTTCATCC
    CCCCACAAAG AATCTTCCAA AACAAAAACA CCCAGGCCCT CCTCTGAGCC CTCAAAGAAG
    GAGATGCTTC CCCCCCCACC TGTGTCATCC TCATCCTCAT CCTCCCAGAA GCCAGCCAAG
    CCTGCACAAA AGAGGCCAAG GCGGGAAGCT GATCCCTCCA GCCAAGACGC TTCCCAAAGT
    GCCAGTAGTA GCAAGGGCAA CCACAAAGAC TCTTCCACTC CCAAGCACAG AAAAGTAGAG
    GGGAAGGGAT CTGGAAGCAG TACAGAGCAC AAGGGATCTT CTGGAGATAG TGCATATCCT
    TTTCTAGTGC CTTCTTTGCC AAATGGTAAC TCTAAACCAG GGAAGCCTCC AGTGAAGCTT
    GACAGACAAC AAGCAGATTT TCACAGGAAG GAGGCCAAAA AGTTGAAGCA AAAAGCAGAG
    TTAATGATGG ACAAGGTTGG GAAGGCTTTT AAGTACTTGG AGGCCGCCTT GTCCTTAATT
    GAGTGTGGGA TTGCCATGGA GTCGGAAAGC CCAGCCTCCA AATCGGCTTA CTCGGTGTAC
    TCCGAAACCG TAGACCTCAT TAAATTTATA ATGTCATTAA AATCCTTCTC AGACGCCTCA
    TCTCCACAAG AGAAAATATT TGCTGTTTTA TGCATGCGTT GTCAGTCCAT TTTGAACATG
    GCGATGTTTC GTTGTAAAAA AGATATAGCA ATAAAGTATT CTCGTGCTCT TAACGAACAC
    TTCAGCAGTT TTTTCAAAGT TGCCCAGGCA CCTTCTCCAT GCATTGCAAG AAACACAGGC
    ACACCGTCCC CTCTCTCTCC AGTTCCTTCT CCTGCCAGCT CCGTAGGGTC CCAGTCCAGT
    GCTGGCAGCA TAGGGAGCAG TGGTATGCCT GTCACCATCC AAAACATGAC ATCTTCTTAT
    GTCACCATTA CTTCCCATGT CCTTACCGCC TTTGACCTCT GGGAACAGGC TGAGGTCCTT
    ACAAGGAAGA ATAAAGAATT TTTTGCTCAG CTCAGCACAA GTGTGTGTGC CTTGGCCCTC
    AACAGCAGTT TGATGGATCT GGTGCACTAT ACAAGACAGG GTTTTCAGCG GCTAAAACAA
    GCAACCAAAA CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29734
    Clone ID Related Accession (Same CDS sequence) XM_005639159.2
    Accession Version XM_005639159.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product AF4/FMR2 family member 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005639159.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAGCCC AGTCCAGCTT GTACAATGAA GACCGGAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC TCACCAAGAG AAAGAGGCAT TTTCTGAAAA GATCCCCCTT
    TTTGGAGAGC CCTACAAGAC AGAAAAAGGT GATGAGCTAT CCAGTCGAAT ACAGAACATG
    TTGGGAAACT ATGAAGAAGT GAAGGAGTTC TTTAGCAGCA AGTCCCACCC TCATCGCTTG
    GATGCCTCTG AAAATAGGTT GGGAAAGCCA AGATATCCTT TATTTCCTGA AAAGGGGAGC
    AGCATTCCAT CCCACTCCTT CCACACGAGT GCCCACCACC AGCCTGTTAA TACTCCTGCC
    TCCGGACCAC TTCCTGTTGG TAACGTAAGC CAGAACCCAA AGATGGCACA GCCAAGAATG
    GAACCAATGT CAAATCCTCA TGTCAAAAGC TATGGCCCAC CAGACAGCCA GCACCCAACC
    CAAGATCGCC TTGGTCAGGA GGTGTGCGGC TCTGGTCATC ACAAAAAAGG TGACCGCAGA
    GCTGATGGAG ACCGCTGTGC TTTGGTGACA GACTCTGCTC CTGAGAGGGA GCTTTCTCCC
    TTACTCTCCT TGCCTTCCCC AGTGCCCCCC CTGTCATCTG TACATTCCAA CCAGCAAGCT
    CTTCCCCGGA CACAAGGAAG CAGCAAGATT CACAGCAGTA ACAGTAATAG TAAAGGCTAT
    TGCCCAGCCA AATCTCCCAA AGACCTAGTA GCAAAGGTCC ATGATAAAGA GACCCCTCAA
    GACAATTTAG TGGCAGTTGC CGGCCTTGGG GTGGCCCCTC CCCAGCCACC TTCTCAGACT
    TTCCCCCCAC CTTCCCTCCC CTCAAAAACT GTTGCGATGC AGCAGAAGCC CACGGCTTAC
    GTCCGGCCCA TGGACGGTCA AGATCAGGCT CCTAGTGAGT CACCTGAACT GAAACCACTG
    CCGGAGGACT ACCGGCAACA GACCTTTGAA AAAACAGACT TAAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCATCTCAG TCGGGTGAGC AGACCTACTC CAATGAAGTT
    CATTGTGTTG AAGAGATTTT GAAGGAAATG ACCCATTCAT GGCCACCTCC TTTGACAGCA
    ATACACACGC CTAGTACTGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG
    CACATTAGTT CTGTGACCCA AAATCAAAAA CAATATGATA CATCTTCAAA AACCCACACT
    AATTCTCAGC AGGGAACATC CATGCTTGAA GATGACCTTC AGCTCAGTGA CAGTGAGGAC
    AGTGACAACG AACAAAGCCC AGAGAAGCCT CCTCCTGCGT CTGCGCCTCC CAGTGCCCCG
    CAGTCGCTTC CAGAAGCAGC GGCCTCAGCA CATTCCAGTA GTGCCGAGTC AGAGAGCACC
    AGTGACTCAG ACAGCTCCTC GGACTCCGAG AGCGAGAGCA GCTCAAGTGA CAGCGAAGAG
    AATGAGCCCC TAGCAGCTCC GACTCCTGAG CCTGAGCCTC CTACAACAAA CAAATGGCAG
    CTGGACAACT GGCTGACCAA AGTCAGCCAA CCGGCAGCCC CACCAGACAC CCAGGAGGGG
    AGCGCAGAGC CCCCGCCTCG GCACCCAGAC AGTAAGGGCA AGGGGGGAGG CGACAGCAGC
    GGCCCCACCA CTGGTCACGA GCTCTCGGAA TCCAAAGATC CTCCCCTCAA GAGCTCCGGC
    AAAGCCCCCC GGGGCTCCTC CGAGGGCCCT CACCCCGGCA GGAGGAGCTG TCCGAAGTCC
    CCGGCCCAAC AGGAGGCCCT GCCGAGGCAG ACTGTGGGAA CCAAACAACC CAAGAAGCCG
    GGCAAGGCCT CCACGCCGGC GGACCCTCGG GCCGGCCTGC AGGTGGAGAG CGAGCCGGGC
    CCCCCTCCGT CCGGCTCCAG AGACCAGCCT TCCAAAGACA AGCCCAAGGT GAAGACGAAA
    GGGAGGCCGC GCGCCGGCGA CAGCAGGGAG CCCAAGCCCG CCGCGCCCAG CCCCGGTGAC
    CGCAAGAAGC ACCAGAGCGG GCCGCTGGCC CCCTCAAAGG CACTCTCGGG CCCCGAGCCC
    ACGAAGGACA ATGCGGGGGC CAGGAGCCCC GAGCACTTTG CCCTGGTTCC CCTGACCCAG
    AGCCAGGGCC CGCCCCACGG CGGTGGCGGC AGGACTAGTG GCTGCCGGCG CGCAGTGGTG
    GTCCAGGAGG ACCGCAGGAA GGACAAGCTC CCGGGGCCTG CCAGAGACAC CAAGCTGCTG
    TCGCCGCTCA GGGACCCTCC TCCCCCACCC ATCTTGATGG TGAAGATAAC CCTGGACCTT
    CTTTCTCGGA TACCCCAGCT TCCTGGGAAA GGGGGCCGGC AGAAGAAGCC AGAGGACAAG
    CAGCCCCCCA CAGGGAAGAA GCCCGATTTG GAGAAGAGAG GCGCAGACAA CTCCAGCAAG
    TTGGTCAAAA AAAGAAAGGC TGAAGCTGAG AAAGACCATG ACAGCAAGAA AGCCAGACTG
    GAGAAAGAAA CCAAGTTGCA GTCATTATCT TCATCCCCCC ACAAAGAATC TTCCAAAACA
    AAAACACCCA GGCCCTCCTC TGAGCCCTCA AAGAAGGAGA TGCTTCCCCC CCCACCTGTG
    TCATCCTCAT CCTCATCCTC CCAGAAGCCA GCCAAGCCTG CACAAAAGAG GCCAAGGCGG
    GAAGCTGATC CCTCCAGCCA AGACGCTTCC CAAAGTGCCA GTAGTAGCAA GGGCAACCAC
    AAAGACTCTT CCACTCCCAA GCACAGAAAA GTAGAGGGGA AGGGATCTGG AAGCAGTACA
    GAGCACAAGG GATCTTCTGG AGATAGTGCA TATCCTTTTC TAGTGCCTTC TTTGCCAAAT
    GGTAACTCTA AACCAGGGAA GCCTCCAGTG AAGCTTGACA GACAACAAGC AGATTTTCAC
    AGGAAGGAGG CCAAAAAGTT GAAGCAAAAA GCAGAGTTAA TGATGGACAA GGTTGGGAAG
    GCTTTTAAGT ACTTGGAGGC CGCCTTGTCC TTAATTGAGT GTGGGATTGC CATGGAGTCG
    GAAAGCCCAG CCTCCAAATC GGCTTACTCG GTGTACTCCG AAACCGTAGA CCTCATTAAA
    TTTATAATGT CATTAAAATC CTTCTCAGAC GCCTCATCTC CACAAGAGAA AATATTTGCT
    GTTTTATGCA TGCGTTGTCA GTCCATTTTG AACATGGCGA TGTTTCGTTG TAAAAAAGAT
    ATAGCAATAA AGTATTCTCG TGCTCTTAAC GAACACTTCA GCAGTTTTTT CAAAGTTGCC
    CAGGCACCTT CTCCATGCAT TGCAAGAAAC ACAGGCACAC CGTCCCCTCT CTCTCCAGTT
    CCTTCTCCTG CCAGCTCCGT AGGGTCCCAG TCCAGTGCTG GCAGCATAGG GAGCAGTGGT
    ATGCCTGTCA CCATCCAAAA CATGACATCT TCTTATGTCA CCATTACTTC CCATGTCCTT
    ACCGCCTTTG ACCTCTGGGA ACAGGCTGAG GTCCTTACAA GGAAGAATAA AGAATTTTTT
    GCTCAGCTCA GCACAAGTGT GTGTGCCTTG GCCCTCAACA GCAGTTTGAT GGATCTGGTG
    CACTATACAA GACAGGGTTT TCAGCGGCTA AAACAAGCAA CCAAAACACC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639216.1
    CDS67..3720
    Translation

    Target ORF information:

    RefSeq Version XM_005639159.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris AF4/FMR2 family, member 1 (AFF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639159.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAGCCC AGTCCAGCTT GTACAATGAA GACCGGAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC TCACCAAGAG AAAGAGGCAT TTTCTGAAAA GATCCCCCTT
    TTTGGAGAGC CCTACAAGAC AGAAAAAGGT GATGAGCTAT CCAGTCGAAT ACAGAACATG
    TTGGGAAACT ATGAAGAAGT GAAGGAGTTC TTTAGCAGCA AGTCCCACCC TCATCGCTTG
    GATGCCTCTG AAAATAGGTT GGGAAAGCCA AGATATCCTT TATTTCCTGA AAAGGGGAGC
    AGCATTCCAT CCCACTCCTT CCACACGAGT GCCCACCACC AGCCTGTTAA TACTCCTGCC
    TCCGGACCAC TTCCTGTTGG TAACGTAAGC CAGAACCCAA AGATGGCACA GCCAAGAATG
    GAACCAATGT CAAATCCTCA TGTCAAAAGC TATGGCCCAC CAGACAGCCA GCACCCAACC
    CAAGATCGCC TTGGTCAGGA GGTGTGCGGC TCTGGTCATC ACAAAAAAGG TGACCGCAGA
    GCTGATGGAG ACCGCTGTGC TTTGGTGACA GACTCTGCTC CTGAGAGGGA GCTTTCTCCC
    TTACTCTCCT TGCCTTCCCC AGTGCCCCCC CTGTCATCTG TACATTCCAA CCAGCAAGCT
    CTTCCCCGGA CACAAGGAAG CAGCAAGATT CACAGCAGTA ACAGTAATAG TAAAGGCTAT
    TGCCCAGCCA AATCTCCCAA AGACCTAGTA GCAAAGGTCC ATGATAAAGA GACCCCTCAA
    GACAATTTAG TGGCAGTTGC CGGCCTTGGG GTGGCCCCTC CCCAGCCACC TTCTCAGACT
    TTCCCCCCAC CTTCCCTCCC CTCAAAAACT GTTGCGATGC AGCAGAAGCC CACGGCTTAC
    GTCCGGCCCA TGGACGGTCA AGATCAGGCT CCTAGTGAGT CACCTGAACT GAAACCACTG
    CCGGAGGACT ACCGGCAACA GACCTTTGAA AAAACAGACT TAAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCATCTCAG TCGGGTGAGC AGACCTACTC CAATGAAGTT
    CATTGTGTTG AAGAGATTTT GAAGGAAATG ACCCATTCAT GGCCACCTCC TTTGACAGCA
    ATACACACGC CTAGTACTGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG
    CACATTAGTT CTGTGACCCA AAATCAAAAA CAATATGATA CATCTTCAAA AACCCACACT
    AATTCTCAGC AGGGAACATC CATGCTTGAA GATGACCTTC AGCTCAGTGA CAGTGAGGAC
    AGTGACAACG AACAAAGCCC AGAGAAGCCT CCTCCTGCGT CTGCGCCTCC CAGTGCCCCG
    CAGTCGCTTC CAGAAGCAGC GGCCTCAGCA CATTCCAGTA GTGCCGAGTC AGAGAGCACC
    AGTGACTCAG ACAGCTCCTC GGACTCCGAG AGCGAGAGCA GCTCAAGTGA CAGCGAAGAG
    AATGAGCCCC TAGCAGCTCC GACTCCTGAG CCTGAGCCTC CTACAACAAA CAAATGGCAG
    CTGGACAACT GGCTGACCAA AGTCAGCCAA CCGGCAGCCC CACCAGACAC CCAGGAGGGG
    AGCGCAGAGC CCCCGCCTCG GCACCCAGAC AGTAAGGGCA AGGGGGGAGG CGACAGCAGC
    GGCCCCACCA CTGGTCACGA GCTCTCGGAA TCCAAAGATC CTCCCCTCAA GAGCTCCGGC
    AAAGCCCCCC GGGGCTCCTC CGAGGGCCCT CACCCCGGCA GGAGGAGCTG TCCGAAGTCC
    CCGGCCCAAC AGGAGGCCCT GCCGAGGCAG ACTGTGGGAA CCAAACAACC CAAGAAGCCG
    GGCAAGGCCT CCACGCCGGC GGACCCTCGG GCCGGCCTGC AGGTGGAGAG CGAGCCGGGC
    CCCCCTCCGT CCGGCTCCAG AGACCAGCCT TCCAAAGACA AGCCCAAGGT GAAGACGAAA
    GGGAGGCCGC GCGCCGGCGA CAGCAGGGAG CCCAAGCCCG CCGCGCCCAG CCCCGGTGAC
    CGCAAGAAGC ACCAGAGCGG GCCGCTGGCC CCCTCAAAGG CACTCTCGGG CCCCGAGCCC
    ACGAAGGACA ATGCGGGGGC CAGGAGCCCC GAGCACTTTG CCCTGGTTCC CCTGACCCAG
    AGCCAGGGCC CGCCCCACGG CGGTGGCGGC AGGACTAGTG GCTGCCGGCG CGCAGTGGTG
    GTCCAGGAGG ACCGCAGGAA GGACAAGCTC CCGGGGCCTG CCAGAGACAC CAAGCTGCTG
    TCGCCGCTCA GGGACCCTCC TCCCCCACCC ATCTTGATGG TGAAGATAAC CCTGGACCTT
    CTTTCTCGGA TACCCCAGCT TCCTGGGAAA GGGGGCCGGC AGAAGAAGCC AGAGGACAAG
    CAGCCCCCCA CAGGGAAGAA GCCCGATTTG GAGAAGAGAG GCGCAGACAA CTCCAGCAAG
    TTGGTCAAAA AAAGAAAGGC TGAAGCTGAG AAAGACCATG ACAGCAAGAA AGCCAGACTG
    GAGAAAGAAA CCAAGTTGCA GTCATTATCT TCATCCCCCC ACAAAGAATC TTCCAAAACA
    AAAACACCCA GGCCCTCCTC TGAGCCCTCA AAGAAGGAGA TGCTTCCCCC CCCACCTGTG
    TCATCCTCAT CCTCATCCTC CCAGAAGCCA GCCAAGCCTG CACAAAAGAG GCCAAGGCGG
    GAAGCTGATC CCTCCAGCCA AGACGCTTCC CAAAGTGCCA GTAGTAGCAA GGGCAACCAC
    AAAGACTCTT CCACTCCCAA GCACAGAAAA GTAGAGGGGA AGGGATCTGG AAGCAGTACA
    GAGCACAAGG GATCTTCTGG AGATAGTGCA TATCCTTTTC TAGTGCCTTC TTTGCCAAAT
    GGTAACTCTA AACCAGGGAA GCCTCCAGTG AAGCTTGACA GACAACAAGC AGATTTTCAC
    AGGAAGGAGG CCAAAAAGTT GAAGCAAAAA GCAGAGTTAA TGATGGACAA GGTTGGGAAG
    GCTTTTAAGT ACTTGGAGGC CGCCTTGTCC TTAATTGAGT GTGGGATTGC CATGGAGTCG
    GAAAGCCCAG CCTCCAAATC GGCTTACTCG GTGTACTCCG AAACCGTAGA CCTCATTAAA
    TTTATAATGT CATTAAAATC CTTCTCAGAC GCCTCATCTC CACAAGAGAA AATATTTGCT
    GTTTTATGCA TGCGTTGTCA GTCCATTTTG AACATGGCGA TGTTTCGTTG TAAAAAAGAT
    ATAGCAATAA AGTATTCTCG TGCTCTTAAC GAACACTTCA GCAGTTTTTT CAAAGTTGCC
    CAGGCACCTT CTCCATGCAT TGCAAGAAAC ACAGGCACAC CGTCCCCTCT CTCTCCAGTT
    CCTTCTCCTG CCAGCTCCGT AGGGTCCCAG TCCAGTGCTG GCAGCATAGG GAGCAGTGGT
    ATGCCTGTCA CCATCCAAAA CATGACATCT TCTTATGTCA CCATTACTTC CCATGTCCTT
    ACCGCCTTTG ACCTCTGGGA ACAGGCTGAG GTCCTTACAA GGAAGAATAA AGAATTTTTT
    GCTCAGCTCA GCACAAGTGT GTGTGCCTTG GCCCTCAACA GCAGTTTGAT GGATCTGGTG
    CACTATACAA GACAGGGTTT TCAGCGGCTA AAACAAGCAA CCAAAACACC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.