AFF1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	AFF1
Entrez Gene ID	100683405
Full Name	AF4/FMR2 family member 1
General protein information	Preferred Names AF4/FMR2 family member 1 Names AF4/FMR2 family member 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022413582.1	XP_022269290.1	AF4/FMR2 family member 1 isoform X2
XM_005639159.2	XP_005639216.1	AF4/FMR2 family member 1 isoform X2
XM_014109957.1	XP_013965432.1	AF4/FMR2 family member 1 isoform X1
XM_022413583.1	XP_022269291.1	AF4/FMR2 family member 1 isoform X3
XM_022413585.1	XP_022269293.1	AF4/FMR2 family member 1 isoform X5
XM_014109958.1	XP_013965433.1	AF4/FMR2 family member 1 isoform X1
XM_022413581.1	XP_022269289.1	AF4/FMR2 family member 1 isoform X1
XM_022413584.1	XP_022269292.1	AF4/FMR2 family member 1 isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AFF1	XP_001156948.2
Mus musculus (house mouse)	Aff1	NP_598680.3
Gallus gallus (chicken)	AFF1	XP_004941157.1
Homo sapiens (human)	AFF1	NP_001160165.1
	AFF1	XP_001095677.2
Canis lupus familiaris (dog)	AFF1	XP_005639216.1
Bos taurus (cattle)	AFF1	NP_001178454.1
Rattus norvegicus (Norway rat)	Aff1	NP_001100676.1

Gene Ontology

The following AFF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe284110	XM_022413581.1 Latest version!	Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe284111	XM_022413583.1 Latest version!	Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe284112	XM_022413584.1 Latest version!	Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe284113	XM_022413585.1 Latest version!	Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe29733	XM_014109958.1 Latest version!	Canis lupus familiaris AF4/FMR2 family, member 1 (AFF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe29733	XM_022413582.1 Latest version!	Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe29733	XM_014109957.1 Latest version!	Canis lupus familiaris AF4/FMR2 family, member 1 (AFF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe29734	XM_005639159.2 Latest version!	Canis lupus familiaris AF4/FMR2 family, member 1 (AFF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFF1 ( XM_022413581.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022269289.1 Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe284110
Clone ID Related Accession (Same CDS sequence)	XM_022413581.1
Accession Version	XM_022413581.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	AF4/FMR2 family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTTA CTGAAAGAGT CCAGAGCAGC AGTAGCAGCA GCTTGTACAA TGAAGACCGG 
AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGAGAAAGAG 
GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCCTACA AGACAGAAAA AGGTGATGAG 
CTATCCAGTC GAATACAGAA CATGTTGGGA AACTATGAAG AAGTGAAGGA GTTCTTTAGC 
AGCAAGTCCC ACCCTCATCG CTTGGATGCC TCTGAAAATA GGTTGGGAAA GCCAAGATAT 
CCTTTATTTC CTGAAAAGGG GAGCAGCATT CCATCCCACT CCTTCCACAC GAGTGCCCAC 
CACCAGCCTG TTAATACTCC TGCCTCCGGA CCACTTCCTG TTGGTAACGT AAGCCAGAAC 
CCAAAGATGG CACAGCCAAG AATGGAACCA ATGTCAAATC CTCATGTCAA AAGCTATGGC 
CCACCAGACA GCCAGCACCC AACCCAAGAT CGCCTTGGTC AGGAGGTGTG CGGCTCTGGT 
CATCACAAAA AAGGTGACCG CAGAGCTGAT GGAGACCGCT GTGCTTTGGT GACAGACTCT 
GCTCCTGAGA GGGAGCTTTC TCCCTTACTC TCCTTGCCTT CCCCAGTGCC CCCCCTGTCA 
TCTGTACATT CCAACCAGCA AGCTCTTCCC CGGACACAAG GAAGCAGCAA GATTCACAGC 
AGTAACAGTA ATAGTAAAGG CTATTGCCCA GCCAAATCTC CCAAAGACCT AGTAGCAAAG 
GTCCATGATA AAGAGACCCC TCAAGACAAT TTAGTGGCAG TTGCCGGCCT TGGGGTGGCC 
CCTCCCCAGC CACCTTCTCA GACTTTCCCC CCACCTTCCC TCCCCTCAAA AACTGTTGCG 
ATGCAGCAGA AGCCCACGGC TTACGTCCGG CCCATGGACG GTCAAGATCA GGCTCCTAGT 
GAGTCACCTG AACTGAAACC ACTGCCGGAG GACTACCGGC AACAGACCTT TGAAAAAACA 
GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACCAAACTGA AGATGCCATC TCAGTCGGGT 
GAGCAGACCT ACTCCAATGA AGTTCATTGT GTTGAAGAGA TTTTGAAGGA AATGACCCAT 
TCATGGCCAC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CTGCTGAGCC CTCCAAATTT 
CCTTTTCCCA CAAAGGATTC TCAGCACATT AGTTCTGTGA CCCAAAATCA AAAACAATAT 
GATACATCTT CAAAAACCCA CACTAATTCT CAGCAGGGAA CATCCATGCT TGAAGATGAC 
CTTCAGCTCA GTGACAGTGA GGACAGTGAC AACGAACAAA GCCCAGAGAA GCCTCCTCCT 
GCGTCTGCGC CTCCCAGGTA TTGTGTGCAT GTTGCCCCGC AGTCGCTTCC AGAAGCAGCG 
GCCTCAGCAC ATTCCAGTAG TGCCGAGTCA GAGAGCACCA GTGACTCAGA CAGCTCCTCG 
GACTCCGAGA GCGAGAGCAG CTCAAGTGAC AGCGAAGAGA ATGAGCCCCT AGCAGCTCCG 
ACTCCTGAGC CTGAGCCTCC TACAACAAAC AAATGGCAGC TGGACAACTG GCTGACCAAA 
GTCAGCCAAC CGGCAGCCCC ACCAGACACC CAGGAGGGGA GCGCAGAGCC CCCGCCTCGG 
CACCCAGACA GTAAGGGCAA GGGGGGAGGC GACAGCAGCG GCCCCACCAC TGGTCACGAG 
CTCTCGGAAT CCAAAGATCC TCCCCTCAAG AGCTCCGGCA AAGCCCCCCG GGGCTCCTCC 
GAGGGCCCTC ACCCCGGCAG GAGGAGCTGT CCGAAGTCCC CGGCCCAACA GGAGGCCCTG 
CCGAGGCAGA CTGTGGGAAC CAAACAACCC AAGAAGCCGG GCAAGGCCTC CACGCCGGCG 
GACCCTCGGG CCGGCCTGCA GGTGGAGAGC GAGCCGGGCC CCCCTCCGTC CGGCTCCAGA 
GACCAGCCTT CCAAAGACAA GCCCAAGGTG AAGACGAAAG GGAGGCCGCG CGCCGGCGAC 
AGCAGGGAGC CCAAGCCCGC CGCGCCCAGC CCCGGTGACC GCAAGAAGCA CCAGAGCGGG 
CCGCTGGCCC CCTCAAAGGC ACTCTCGGGC CCCGAGCCCA CGAAGGACAA TGCGGGGGCC 
AGGAGCCCCG AGCACTTTGC CCTGGTTCCC CTGACCCAGA GCCAGGGCCC GCCCCACGGC 
GGTGGCGGCA GGACTAGTGG CTGCCGGCGC GCAGTGGTGG TCCAGGAGGA CCGCAGGAAG 
GACAAGCTCC CGGGGCCTGC CAGAGACACC AAGCTGCTGT CGCCGCTCAG GGACCCTCCT 
CCCCCACCCA TCTTGATGGT GAAGATAACC CTGGACCTTC TTTCTCGGAT ACCCCAGCTT 
CCTGGGAAAG GGGGCCGGCA GAAGAAGCCA GAGGACAAGC AGCCCCCCAC AGGGAAGAAG 
CCCGATTTGG AGAAGAGAGG CGCAGACAAC TCCAGCAAGT TGGTCAAAAA AAGAAAGGCT 
GAAGCTGAGA AAGACCATGA CAGCAAGAAA GCCAGACTGG AGAAAGAAAC CAAGTTGCAG 
TCATTATCTT CATCCCCCCA CAAAGAATCT TCCAAAACAA AAACACCCAG GCCCTCCTCT 
GAGCCCTCAA AGAAGGAGAT GCTTCCCCCC CCACCTGTGT CATCCTCATC CTCATCCTCC 
CAGAAGCCAG CCAAGCCTGC ACAAAAGAGG CCAAGGCGGG AAGCTGATCC CTCCAGCCAA 
GACGCTTCCC AAAGTGCCAG TAGTAGCAAG GGCAACCACA AAGACTCTTC CACTCCCAAG 
CACAGAAAAG TAGAGGGGAA GGGATCTGGA AGCAGTACAG AGCACAAGGG ATCTTCTGGA 
GATAGTGCAT ATCCTTTTCT AGTGCCTTCT TTGCCAAATG GTAACTCTAA ACCAGGGAAG 
CCTCCAGTGA AGCTTGACAG ACAACAAGCA GATTTTCACA GGAAGGAGGC CAAAAAGTTG 
AAGCAAAAAG CAGAGTTAAT GATGGACAAG GTTGGGAAGG CTTTTAAGTA CTTGGAGGCC 
GCCTTGTCCT TAATTGAGTG TGGGATTGCC ATGGAGTCGG AAAGCCCAGC CTCCAAATCG 
GCTTACTCGG TGTACTCCGA AACCGTAGAC CTCATTAAAT TTATAATGTC ATTAAAATCC 
TTCTCAGACG CCTCATCTCC ACAAGAGAAA ATATTTGCTG TTTTATGCAT GCGTTGTCAG 
TCCATTTTGA ACATGGCGAT GTTTCGTTGT AAAAAAGATA TAGCAATAAA GTATTCTCGT 
GCTCTTAACG AACACTTCAG CAGTTTTTTC AAAGTTGCCC AGGCACCTTC TCCATGCATT 
GCAAGAAACA CAGGCACACC GTCCCCTCTC TCTCCAGTTC CTTCTCCTGC CAGCTCCGTA 
GGGTCCCAGT CCAGTGCTGG CAGCATAGGG AGCAGTGGTA TGCCTGTCAC CATCCAAAAC 
ATGACATCTT CTTATGTCAC CATTACTTCC CATGTCCTTA CCGCCTTTGA CCTCTGGGAA 
CAGGCTGAGG TCCTTACAAG GAAGAATAAA GAATTTTTTG CTCAGCTCAG CACAAGTGTG 
TGTGCCTTGG CCCTCAACAG CAGTTTGATG GATCTGGTGC ACTATACAAG ACAGGGTTTT 
CAGCGGCTAA AACAAGCAAC CAAAACACCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022269289.1
CDS	279..3971
Translation	MAFTERVQSSSSSSLYNEDRNLLRIREKERRNQEAHQEKEAFSEKIPLFGEPYKTEKGDELSSRIQNMLGNYEEVKEFFSSKSHPHRLDASENRLGKPRYPLFPEKGSSIPSHSFHTSAHHQPVNTPASGPLPVGNVSQNPKMAQPRMEPMSNPHVKSYGPPDSQHPTQDRLGQEVCGSGHHKKGDRRADGDRCALVTDSAPERELSPLLSLPSPVPPLSSVHSNQQALPRTQGSSKIHSSNSNSKGYCPAKSPKDLVAKVHDKETPQDNLVAVAGLGVAPPQPPSQTFPPPSLPSKTVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSGEQTYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHISSVTQNQKQYDTSSKTHTNSQQGTSMLEDDLQLSDSEDSDNEQSPEKPPPASAPPRYCVHVAPQSLPEAAASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLAAPTPEPEPPTTNKWQLDNWLTKVSQPAAPPDTQEGSAEPPPRHPDSKGKGGGDSSGPTTGHELSESKDPPLKSSGKAPRGSSEGPHPGRRSCPKSPAQQEALPRQTVGTKQPKKPGKASTPADPRAGLQVESEPGPPPSGSRDQPSKDKPKVKTKGRPRAGDSREPKPAAPSPGDRKKHQSGPLAPSKALSGPEPTKDNAGARSPEHFALVPLTQSQGPPHGGGGRTSGCRRAVVVQEDRRKDKLPGPARDTKLLSPLRDPPPPPILMVKITLDLLSRIPQLPGKGGRQKKPEDKQPPTGKKPDLEKRGADNSSKLVKKRKAEAEKDHDSKKARLEKETKLQSLSSSPHKESSKTKTPRPSSEPSKKEMLPPPPVSSSSSSSQKPAKPAQKRPRREADPSSQDASQSASSSKGNHKDSSTPKHRKVEGKGSGSSTEHKGSSGDSAYPFLVPSLPNGNSKPGKPPVKLDRQQADFHRKEAKKLKQKAELMMDKVGKAFKYLEAALSLIECGIAMESESPASKSAYSVYSETVDLIKFIMSLKSFSDASSPQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRALNEHFSSFFKVAQAPSPCIARNTGTPSPLSPVPSPASSVGSQSSAGSIGSSGMPVTIQNMTSSYVTITSHVLTAFDLWEQAEVLTRKNKEFFAQLSTSVCALALNSSLMDLVHYTRQGFQRLKQATKTP

Target ORF information:

RefSeq Version	XM_022413581.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022413581.1

ORF Insert Sequence:

ATGGCGTTTA CTGAAAGAGT CCAGAGCAGC AGTAGCAGCA GCTTGTACAA TGAAGACCGG 
AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGAGAAAGAG 
GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCCTACA AGACAGAAAA AGGTGATGAG 
CTATCCAGTC GAATACAGAA CATGTTGGGA AACTATGAAG AAGTGAAGGA GTTCTTTAGC 
AGCAAGTCCC ACCCTCATCG CTTGGATGCC TCTGAAAATA GGTTGGGAAA GCCAAGATAT 
CCTTTATTTC CTGAAAAGGG GAGCAGCATT CCATCCCACT CCTTCCACAC GAGTGCCCAC 
CACCAGCCTG TTAATACTCC TGCCTCCGGA CCACTTCCTG TTGGTAACGT AAGCCAGAAC 
CCAAAGATGG CACAGCCAAG AATGGAACCA ATGTCAAATC CTCATGTCAA AAGCTATGGC 
CCACCAGACA GCCAGCACCC AACCCAAGAT CGCCTTGGTC AGGAGGTGTG CGGCTCTGGT 
CATCACAAAA AAGGTGACCG CAGAGCTGAT GGAGACCGCT GTGCTTTGGT GACAGACTCT 
GCTCCTGAGA GGGAGCTTTC TCCCTTACTC TCCTTGCCTT CCCCAGTGCC CCCCCTGTCA 
TCTGTACATT CCAACCAGCA AGCTCTTCCC CGGACACAAG GAAGCAGCAA GATTCACAGC 
AGTAACAGTA ATAGTAAAGG CTATTGCCCA GCCAAATCTC CCAAAGACCT AGTAGCAAAG 
GTCCATGATA AAGAGACCCC TCAAGACAAT TTAGTGGCAG TTGCCGGCCT TGGGGTGGCC 
CCTCCCCAGC CACCTTCTCA GACTTTCCCC CCACCTTCCC TCCCCTCAAA AACTGTTGCG 
ATGCAGCAGA AGCCCACGGC TTACGTCCGG CCCATGGACG GTCAAGATCA GGCTCCTAGT 
GAGTCACCTG AACTGAAACC ACTGCCGGAG GACTACCGGC AACAGACCTT TGAAAAAACA 
GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACCAAACTGA AGATGCCATC TCAGTCGGGT 
GAGCAGACCT ACTCCAATGA AGTTCATTGT GTTGAAGAGA TTTTGAAGGA AATGACCCAT 
TCATGGCCAC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CTGCTGAGCC CTCCAAATTT 
CCTTTTCCCA CAAAGGATTC TCAGCACATT AGTTCTGTGA CCCAAAATCA AAAACAATAT 
GATACATCTT CAAAAACCCA CACTAATTCT CAGCAGGGAA CATCCATGCT TGAAGATGAC 
CTTCAGCTCA GTGACAGTGA GGACAGTGAC AACGAACAAA GCCCAGAGAA GCCTCCTCCT 
GCGTCTGCGC CTCCCAGGTA TTGTGTGCAT GTTGCCCCGC AGTCGCTTCC AGAAGCAGCG 
GCCTCAGCAC ATTCCAGTAG TGCCGAGTCA GAGAGCACCA GTGACTCAGA CAGCTCCTCG 
GACTCCGAGA GCGAGAGCAG CTCAAGTGAC AGCGAAGAGA ATGAGCCCCT AGCAGCTCCG 
ACTCCTGAGC CTGAGCCTCC TACAACAAAC AAATGGCAGC TGGACAACTG GCTGACCAAA 
GTCAGCCAAC CGGCAGCCCC ACCAGACACC CAGGAGGGGA GCGCAGAGCC CCCGCCTCGG 
CACCCAGACA GTAAGGGCAA GGGGGGAGGC GACAGCAGCG GCCCCACCAC TGGTCACGAG 
CTCTCGGAAT CCAAAGATCC TCCCCTCAAG AGCTCCGGCA AAGCCCCCCG GGGCTCCTCC 
GAGGGCCCTC ACCCCGGCAG GAGGAGCTGT CCGAAGTCCC CGGCCCAACA GGAGGCCCTG 
CCGAGGCAGA CTGTGGGAAC CAAACAACCC AAGAAGCCGG GCAAGGCCTC CACGCCGGCG 
GACCCTCGGG CCGGCCTGCA GGTGGAGAGC GAGCCGGGCC CCCCTCCGTC CGGCTCCAGA 
GACCAGCCTT CCAAAGACAA GCCCAAGGTG AAGACGAAAG GGAGGCCGCG CGCCGGCGAC 
AGCAGGGAGC CCAAGCCCGC CGCGCCCAGC CCCGGTGACC GCAAGAAGCA CCAGAGCGGG 
CCGCTGGCCC CCTCAAAGGC ACTCTCGGGC CCCGAGCCCA CGAAGGACAA TGCGGGGGCC 
AGGAGCCCCG AGCACTTTGC CCTGGTTCCC CTGACCCAGA GCCAGGGCCC GCCCCACGGC 
GGTGGCGGCA GGACTAGTGG CTGCCGGCGC GCAGTGGTGG TCCAGGAGGA CCGCAGGAAG 
GACAAGCTCC CGGGGCCTGC CAGAGACACC AAGCTGCTGT CGCCGCTCAG GGACCCTCCT 
CCCCCACCCA TCTTGATGGT GAAGATAACC CTGGACCTTC TTTCTCGGAT ACCCCAGCTT 
CCTGGGAAAG GGGGCCGGCA GAAGAAGCCA GAGGACAAGC AGCCCCCCAC AGGGAAGAAG 
CCCGATTTGG AGAAGAGAGG CGCAGACAAC TCCAGCAAGT TGGTCAAAAA AAGAAAGGCT 
GAAGCTGAGA AAGACCATGA CAGCAAGAAA GCCAGACTGG AGAAAGAAAC CAAGTTGCAG 
TCATTATCTT CATCCCCCCA CAAAGAATCT TCCAAAACAA AAACACCCAG GCCCTCCTCT 
GAGCCCTCAA AGAAGGAGAT GCTTCCCCCC CCACCTGTGT CATCCTCATC CTCATCCTCC 
CAGAAGCCAG CCAAGCCTGC ACAAAAGAGG CCAAGGCGGG AAGCTGATCC CTCCAGCCAA 
GACGCTTCCC AAAGTGCCAG TAGTAGCAAG GGCAACCACA AAGACTCTTC CACTCCCAAG 
CACAGAAAAG TAGAGGGGAA GGGATCTGGA AGCAGTACAG AGCACAAGGG ATCTTCTGGA 
GATAGTGCAT ATCCTTTTCT AGTGCCTTCT TTGCCAAATG GTAACTCTAA ACCAGGGAAG 
CCTCCAGTGA AGCTTGACAG ACAACAAGCA GATTTTCACA GGAAGGAGGC CAAAAAGTTG 
AAGCAAAAAG CAGAGTTAAT GATGGACAAG GTTGGGAAGG CTTTTAAGTA CTTGGAGGCC 
GCCTTGTCCT TAATTGAGTG TGGGATTGCC ATGGAGTCGG AAAGCCCAGC CTCCAAATCG 
GCTTACTCGG TGTACTCCGA AACCGTAGAC CTCATTAAAT TTATAATGTC ATTAAAATCC 
TTCTCAGACG CCTCATCTCC ACAAGAGAAA ATATTTGCTG TTTTATGCAT GCGTTGTCAG 
TCCATTTTGA ACATGGCGAT GTTTCGTTGT AAAAAAGATA TAGCAATAAA GTATTCTCGT 
GCTCTTAACG AACACTTCAG CAGTTTTTTC AAAGTTGCCC AGGCACCTTC TCCATGCATT 
GCAAGAAACA CAGGCACACC GTCCCCTCTC TCTCCAGTTC CTTCTCCTGC CAGCTCCGTA 
GGGTCCCAGT CCAGTGCTGG CAGCATAGGG AGCAGTGGTA TGCCTGTCAC CATCCAAAAC 
ATGACATCTT CTTATGTCAC CATTACTTCC CATGTCCTTA CCGCCTTTGA CCTCTGGGAA 
CAGGCTGAGG TCCTTACAAG GAAGAATAAA GAATTTTTTG CTCAGCTCAG CACAAGTGTG 
TGTGCCTTGG CCCTCAACAG CAGTTTGATG GATCTGGTGC ACTATACAAG ACAGGGTTTT 
CAGCGGCTAA AACAAGCAAC CAAAACACCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_022413583.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022269291.1 Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe284111
Clone ID Related Accession (Same CDS sequence)	XM_022413583.1
Accession Version	XM_022413583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	AF4/FMR2 family member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTCT TCAAATGCAT CTTGTACAAT GAAGACCGGA ACCTGCTTCG AATTAGAGAG 
AAGGAAAGAC GCAACCAGGA AGCTCACCAA GAGAAAGAGG CATTTTCTGA AAAGATCCCC 
CTTTTTGGAG AGCCCTACAA GACAGAAAAA GGTGATGAGC TATCCAGTCG AATACAGAAC 
ATGTTGGGAA ACTATGAAGA AGTGAAGGAG TTCTTTAGCA GCAAGTCCCA CCCTCATCGC 
TTGGATGCCT CTGAAAATAG GTTGGGAAAG CCAAGATATC CTTTATTTCC TGAAAAGGGG 
AGCAGCATTC CATCCCACTC CTTCCACACG AGTGCCCACC ACCAGCCTGT TAATACTCCT 
GCCTCCGGAC CACTTCCTGT TGGTAACGTA AGCCAGAACC CAAAGATGGC ACAGCCAAGA 
ATGGAACCAA TGTCAAATCC TCATGTCAAA AGCTATGGCC CACCAGACAG CCAGCACCCA 
ACCCAAGATC GCCTTGGTCA GGAGGTGTGC GGCTCTGGTC ATCACAAAAA AGGTGACCGC 
AGAGCTGATG GAGACCGCTG TGCTTTGGTG ACAGACTCTG CTCCTGAGAG GGAGCTTTCT 
CCCTTACTCT CCTTGCCTTC CCCAGTGCCC CCCCTGTCAT CTGTACATTC CAACCAGCAA 
GCTCTTCCCC GGACACAAGG AAGCAGCAAG ATTCACAGCA GTAACAGTAA TAGTAAAGGC 
TATTGCCCAG CCAAATCTCC CAAAGACCTA GTAGCAAAGG TCCATGATAA AGAGACCCCT 
CAAGACAATT TAGTGGCAGT TGCCGGCCTT GGGGTGGCCC CTCCCCAGCC ACCTTCTCAG 
ACTTTCCCCC CACCTTCCCT CCCCTCAAAA ACTGTTGCGA TGCAGCAGAA GCCCACGGCT 
TACGTCCGGC CCATGGACGG TCAAGATCAG GCTCCTAGTG AGTCACCTGA ACTGAAACCA 
CTGCCGGAGG ACTACCGGCA ACAGACCTTT GAAAAAACAG ACTTAAAAGT GCCTGCCAAA 
GCCAAGCTCA CCAAACTGAA GATGCCATCT CAGTCGGGTG AGCAGACCTA CTCCAATGAA 
GTTCATTGTG TTGAAGAGAT TTTGAAGGAA ATGACCCATT CATGGCCACC TCCTTTGACA 
GCAATACACA CGCCTAGTAC TGCTGAGCCC TCCAAATTTC CTTTTCCCAC AAAGGATTCT 
CAGCACATTA GTTCTGTGAC CCAAAATCAA AAACAATATG ATACATCTTC AAAAACCCAC 
ACTAATTCTC AGCAGGGAAC ATCCATGCTT GAAGATGACC TTCAGCTCAG TGACAGTGAG 
GACAGTGACA ACGAACAAAG CCCAGAGAAG CCTCCTCCTG CGTCTGCGCC TCCCAGGTAT 
TGTGTGCATG TTGCCCCGCA GTCGCTTCCA GAAGCAGCGG CCTCAGCACA TTCCAGTAGT 
GCCGAGTCAG AGAGCACCAG TGACTCAGAC AGCTCCTCGG ACTCCGAGAG CGAGAGCAGC 
TCAAGTGACA GCGAAGAGAA TGAGCCCCTA GCAGCTCCGA CTCCTGAGCC TGAGCCTCCT 
ACAACAAACA AATGGCAGCT GGACAACTGG CTGACCAAAG TCAGCCAACC GGCAGCCCCA 
CCAGACACCC AGGAGGGGAG CGCAGAGCCC CCGCCTCGGC ACCCAGACAG TAAGGGCAAG 
GGGGGAGGCG ACAGCAGCGG CCCCACCACT GGTCACGAGC TCTCGGAATC CAAAGATCCT 
CCCCTCAAGA GCTCCGGCAA AGCCCCCCGG GGCTCCTCCG AGGGCCCTCA CCCCGGCAGG 
AGGAGCTGTC CGAAGTCCCC GGCCCAACAG GAGGCCCTGC CGAGGCAGAC TGTGGGAACC 
AAACAACCCA AGAAGCCGGG CAAGGCCTCC ACGCCGGCGG ACCCTCGGGC CGGCCTGCAG 
GTGGAGAGCG AGCCGGGCCC CCCTCCGTCC GGCTCCAGAG ACCAGCCTTC CAAAGACAAG 
CCCAAGGTGA AGACGAAAGG GAGGCCGCGC GCCGGCGACA GCAGGGAGCC CAAGCCCGCC 
GCGCCCAGCC CCGGTGACCG CAAGAAGCAC CAGAGCGGGC CGCTGGCCCC CTCAAAGGCA 
CTCTCGGGCC CCGAGCCCAC GAAGGACAAT GCGGGGGCCA GGAGCCCCGA GCACTTTGCC 
CTGGTTCCCC TGACCCAGAG CCAGGGCCCG CCCCACGGCG GTGGCGGCAG GACTAGTGGC 
TGCCGGCGCG CAGTGGTGGT CCAGGAGGAC CGCAGGAAGG ACAAGCTCCC GGGGCCTGCC 
AGAGACACCA AGCTGCTGTC GCCGCTCAGG GACCCTCCTC CCCCACCCAT CTTGATGGTG 
AAGATAACCC TGGACCTTCT TTCTCGGATA CCCCAGCTTC CTGGGAAAGG GGGCCGGCAG 
AAGAAGCCAG AGGACAAGCA GCCCCCCACA GGGAAGAAGC CCGATTTGGA GAAGAGAGGC 
GCAGACAACT CCAGCAAGTT GGTCAAAAAA AGAAAGGCTG AAGCTGAGAA AGACCATGAC 
AGCAAGAAAG CCAGACTGGA GAAAGAAACC AAGTTGCAGT CATTATCTTC ATCCCCCCAC 
AAAGAATCTT CCAAAACAAA AACACCCAGG CCCTCCTCTG AGCCCTCAAA GAAGGAGATG 
CTTCCCCCCC CACCTGTGTC ATCCTCATCC TCATCCTCCC AGAAGCCAGC CAAGCCTGCA 
CAAAAGAGGC CAAGGCGGGA AGCTGATCCC TCCAGCCAAG ACGCTTCCCA AAGTGCCAGT 
AGTAGCAAGG GCAACCACAA AGACTCTTCC ACTCCCAAGC ACAGAAAAGT AGAGGGGAAG 
GGATCTGGAA GCAGTACAGA GCACAAGGGA TCTTCTGGAG ATAGTGCATA TCCTTTTCTA 
GTGCCTTCTT TGCCAAATGG TAACTCTAAA CCAGGGAAGC CTCCAGTGAA GCTTGACAGA 
CAACAAGCAG ATTTTCACAG GAAGGAGGCC AAAAAGTTGA AGCAAAAAGC AGAGTTAATG 
ATGGACAAGG TTGGGAAGGC TTTTAAGTAC TTGGAGGCCG CCTTGTCCTT AATTGAGTGT 
GGGATTGCCA TGGAGTCGGA AAGCCCAGCC TCCAAATCGG CTTACTCGGT GTACTCCGAA 
ACCGTAGACC TCATTAAATT TATAATGTCA TTAAAATCCT TCTCAGACGC CTCATCTCCA 
CAAGAGAAAA TATTTGCTGT TTTATGCATG CGTTGTCAGT CCATTTTGAA CATGGCGATG 
TTTCGTTGTA AAAAAGATAT AGCAATAAAG TATTCTCGTG CTCTTAACGA ACACTTCAGC 
AGTTTTTTCA AAGTTGCCCA GGCACCTTCT CCATGCATTG CAAGAAACAC AGGCACACCG 
TCCCCTCTCT CTCCAGTTCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC CAGTGCTGGC 
AGCATAGGGA GCAGTGGTAT GCCTGTCACC ATCCAAAACA TGACATCTTC TTATGTCACC 
ATTACTTCCC ATGTCCTTAC CGCCTTTGAC CTCTGGGAAC AGGCTGAGGT CCTTACAAGG 
AAGAATAAAG AATTTTTTGC TCAGCTCAGC ACAAGTGTGT GTGCCTTGGC CCTCAACAGC 
AGTTTGATGG ATCTGGTGCA CTATACAAGA CAGGGTTTTC AGCGGCTAAA ACAAGCAACC 
AAAACACCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022269291.1
CDS	109..3780
Translation	MSFFKCILYNEDRNLLRIREKERRNQEAHQEKEAFSEKIPLFGEPYKTEKGDELSSRIQNMLGNYEEVKEFFSSKSHPHRLDASENRLGKPRYPLFPEKGSSIPSHSFHTSAHHQPVNTPASGPLPVGNVSQNPKMAQPRMEPMSNPHVKSYGPPDSQHPTQDRLGQEVCGSGHHKKGDRRADGDRCALVTDSAPERELSPLLSLPSPVPPLSSVHSNQQALPRTQGSSKIHSSNSNSKGYCPAKSPKDLVAKVHDKETPQDNLVAVAGLGVAPPQPPSQTFPPPSLPSKTVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSGEQTYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHISSVTQNQKQYDTSSKTHTNSQQGTSMLEDDLQLSDSEDSDNEQSPEKPPPASAPPRYCVHVAPQSLPEAAASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLAAPTPEPEPPTTNKWQLDNWLTKVSQPAAPPDTQEGSAEPPPRHPDSKGKGGGDSSGPTTGHELSESKDPPLKSSGKAPRGSSEGPHPGRRSCPKSPAQQEALPRQTVGTKQPKKPGKASTPADPRAGLQVESEPGPPPSGSRDQPSKDKPKVKTKGRPRAGDSREPKPAAPSPGDRKKHQSGPLAPSKALSGPEPTKDNAGARSPEHFALVPLTQSQGPPHGGGGRTSGCRRAVVVQEDRRKDKLPGPARDTKLLSPLRDPPPPPILMVKITLDLLSRIPQLPGKGGRQKKPEDKQPPTGKKPDLEKRGADNSSKLVKKRKAEAEKDHDSKKARLEKETKLQSLSSSPHKESSKTKTPRPSSEPSKKEMLPPPPVSSSSSSSQKPAKPAQKRPRREADPSSQDASQSASSSKGNHKDSSTPKHRKVEGKGSGSSTEHKGSSGDSAYPFLVPSLPNGNSKPGKPPVKLDRQQADFHRKEAKKLKQKAELMMDKVGKAFKYLEAALSLIECGIAMESESPASKSAYSVYSETVDLIKFIMSLKSFSDASSPQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRALNEHFSSFFKVAQAPSPCIARNTGTPSPLSPVPSPASSVGSQSSAGSIGSSGMPVTIQNMTSSYVTITSHVLTAFDLWEQAEVLTRKNKEFFAQLSTSVCALALNSSLMDLVHYTRQGFQRLKQATKTP

Target ORF information:

RefSeq Version	XM_022413583.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022413583.1

ORF Insert Sequence:

ATGTCTTTCT TCAAATGCAT CTTGTACAAT GAAGACCGGA ACCTGCTTCG AATTAGAGAG 
AAGGAAAGAC GCAACCAGGA AGCTCACCAA GAGAAAGAGG CATTTTCTGA AAAGATCCCC 
CTTTTTGGAG AGCCCTACAA GACAGAAAAA GGTGATGAGC TATCCAGTCG AATACAGAAC 
ATGTTGGGAA ACTATGAAGA AGTGAAGGAG TTCTTTAGCA GCAAGTCCCA CCCTCATCGC 
TTGGATGCCT CTGAAAATAG GTTGGGAAAG CCAAGATATC CTTTATTTCC TGAAAAGGGG 
AGCAGCATTC CATCCCACTC CTTCCACACG AGTGCCCACC ACCAGCCTGT TAATACTCCT 
GCCTCCGGAC CACTTCCTGT TGGTAACGTA AGCCAGAACC CAAAGATGGC ACAGCCAAGA 
ATGGAACCAA TGTCAAATCC TCATGTCAAA AGCTATGGCC CACCAGACAG CCAGCACCCA 
ACCCAAGATC GCCTTGGTCA GGAGGTGTGC GGCTCTGGTC ATCACAAAAA AGGTGACCGC 
AGAGCTGATG GAGACCGCTG TGCTTTGGTG ACAGACTCTG CTCCTGAGAG GGAGCTTTCT 
CCCTTACTCT CCTTGCCTTC CCCAGTGCCC CCCCTGTCAT CTGTACATTC CAACCAGCAA 
GCTCTTCCCC GGACACAAGG AAGCAGCAAG ATTCACAGCA GTAACAGTAA TAGTAAAGGC 
TATTGCCCAG CCAAATCTCC CAAAGACCTA GTAGCAAAGG TCCATGATAA AGAGACCCCT 
CAAGACAATT TAGTGGCAGT TGCCGGCCTT GGGGTGGCCC CTCCCCAGCC ACCTTCTCAG 
ACTTTCCCCC CACCTTCCCT CCCCTCAAAA ACTGTTGCGA TGCAGCAGAA GCCCACGGCT 
TACGTCCGGC CCATGGACGG TCAAGATCAG GCTCCTAGTG AGTCACCTGA ACTGAAACCA 
CTGCCGGAGG ACTACCGGCA ACAGACCTTT GAAAAAACAG ACTTAAAAGT GCCTGCCAAA 
GCCAAGCTCA CCAAACTGAA GATGCCATCT CAGTCGGGTG AGCAGACCTA CTCCAATGAA 
GTTCATTGTG TTGAAGAGAT TTTGAAGGAA ATGACCCATT CATGGCCACC TCCTTTGACA 
GCAATACACA CGCCTAGTAC TGCTGAGCCC TCCAAATTTC CTTTTCCCAC AAAGGATTCT 
CAGCACATTA GTTCTGTGAC CCAAAATCAA AAACAATATG ATACATCTTC AAAAACCCAC 
ACTAATTCTC AGCAGGGAAC ATCCATGCTT GAAGATGACC TTCAGCTCAG TGACAGTGAG 
GACAGTGACA ACGAACAAAG CCCAGAGAAG CCTCCTCCTG CGTCTGCGCC TCCCAGGTAT 
TGTGTGCATG TTGCCCCGCA GTCGCTTCCA GAAGCAGCGG CCTCAGCACA TTCCAGTAGT 
GCCGAGTCAG AGAGCACCAG TGACTCAGAC AGCTCCTCGG ACTCCGAGAG CGAGAGCAGC 
TCAAGTGACA GCGAAGAGAA TGAGCCCCTA GCAGCTCCGA CTCCTGAGCC TGAGCCTCCT 
ACAACAAACA AATGGCAGCT GGACAACTGG CTGACCAAAG TCAGCCAACC GGCAGCCCCA 
CCAGACACCC AGGAGGGGAG CGCAGAGCCC CCGCCTCGGC ACCCAGACAG TAAGGGCAAG 
GGGGGAGGCG ACAGCAGCGG CCCCACCACT GGTCACGAGC TCTCGGAATC CAAAGATCCT 
CCCCTCAAGA GCTCCGGCAA AGCCCCCCGG GGCTCCTCCG AGGGCCCTCA CCCCGGCAGG 
AGGAGCTGTC CGAAGTCCCC GGCCCAACAG GAGGCCCTGC CGAGGCAGAC TGTGGGAACC 
AAACAACCCA AGAAGCCGGG CAAGGCCTCC ACGCCGGCGG ACCCTCGGGC CGGCCTGCAG 
GTGGAGAGCG AGCCGGGCCC CCCTCCGTCC GGCTCCAGAG ACCAGCCTTC CAAAGACAAG 
CCCAAGGTGA AGACGAAAGG GAGGCCGCGC GCCGGCGACA GCAGGGAGCC CAAGCCCGCC 
GCGCCCAGCC CCGGTGACCG CAAGAAGCAC CAGAGCGGGC CGCTGGCCCC CTCAAAGGCA 
CTCTCGGGCC CCGAGCCCAC GAAGGACAAT GCGGGGGCCA GGAGCCCCGA GCACTTTGCC 
CTGGTTCCCC TGACCCAGAG CCAGGGCCCG CCCCACGGCG GTGGCGGCAG GACTAGTGGC 
TGCCGGCGCG CAGTGGTGGT CCAGGAGGAC CGCAGGAAGG ACAAGCTCCC GGGGCCTGCC 
AGAGACACCA AGCTGCTGTC GCCGCTCAGG GACCCTCCTC CCCCACCCAT CTTGATGGTG 
AAGATAACCC TGGACCTTCT TTCTCGGATA CCCCAGCTTC CTGGGAAAGG GGGCCGGCAG 
AAGAAGCCAG AGGACAAGCA GCCCCCCACA GGGAAGAAGC CCGATTTGGA GAAGAGAGGC 
GCAGACAACT CCAGCAAGTT GGTCAAAAAA AGAAAGGCTG AAGCTGAGAA AGACCATGAC 
AGCAAGAAAG CCAGACTGGA GAAAGAAACC AAGTTGCAGT CATTATCTTC ATCCCCCCAC 
AAAGAATCTT CCAAAACAAA AACACCCAGG CCCTCCTCTG AGCCCTCAAA GAAGGAGATG 
CTTCCCCCCC CACCTGTGTC ATCCTCATCC TCATCCTCCC AGAAGCCAGC CAAGCCTGCA 
CAAAAGAGGC CAAGGCGGGA AGCTGATCCC TCCAGCCAAG ACGCTTCCCA AAGTGCCAGT 
AGTAGCAAGG GCAACCACAA AGACTCTTCC ACTCCCAAGC ACAGAAAAGT AGAGGGGAAG 
GGATCTGGAA GCAGTACAGA GCACAAGGGA TCTTCTGGAG ATAGTGCATA TCCTTTTCTA 
GTGCCTTCTT TGCCAAATGG TAACTCTAAA CCAGGGAAGC CTCCAGTGAA GCTTGACAGA 
CAACAAGCAG ATTTTCACAG GAAGGAGGCC AAAAAGTTGA AGCAAAAAGC AGAGTTAATG 
ATGGACAAGG TTGGGAAGGC TTTTAAGTAC TTGGAGGCCG CCTTGTCCTT AATTGAGTGT 
GGGATTGCCA TGGAGTCGGA AAGCCCAGCC TCCAAATCGG CTTACTCGGT GTACTCCGAA 
ACCGTAGACC TCATTAAATT TATAATGTCA TTAAAATCCT TCTCAGACGC CTCATCTCCA 
CAAGAGAAAA TATTTGCTGT TTTATGCATG CGTTGTCAGT CCATTTTGAA CATGGCGATG 
TTTCGTTGTA AAAAAGATAT AGCAATAAAG TATTCTCGTG CTCTTAACGA ACACTTCAGC 
AGTTTTTTCA AAGTTGCCCA GGCACCTTCT CCATGCATTG CAAGAAACAC AGGCACACCG 
TCCCCTCTCT CTCCAGTTCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC CAGTGCTGGC 
AGCATAGGGA GCAGTGGTAT GCCTGTCACC ATCCAAAACA TGACATCTTC TTATGTCACC 
ATTACTTCCC ATGTCCTTAC CGCCTTTGAC CTCTGGGAAC AGGCTGAGGT CCTTACAAGG 
AAGAATAAAG AATTTTTTGC TCAGCTCAGC ACAAGTGTGT GTGCCTTGGC CCTCAACAGC 
AGTTTGATGG ATCTGGTGCA CTATACAAGA CAGGGTTTTC AGCGGCTAAA ACAAGCAACC 
AAAACACCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_022413584.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022269292.1 Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA.

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CloneID	OCe284112
Clone ID Related Accession (Same CDS sequence)	XM_022413584.1
Accession Version	XM_022413584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	AF4/FMR2 family member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCCC AGTCCAGCTT GTACAATGAA GACCGGAACC TGCTTCGAAT TAGAGAGAAG 
GAAAGACGCA ACCAGGAAGC TCACCAAGAG AAAGAGGCAT TTTCTGAAAA GATCCCCCTT 
TTTGGAGAGC CCTACAAGAC AGAAAAAGGT GATGAGCTAT CCAGTCGAAT ACAGAACATG 
TTGGGAAACT ATGAAGAAGT GAAGGAGTTC TTTAGCAGCA AGTCCCACCC TCATCGCTTG 
GATGCCTCTG AAAATAGGTT GGGAAAGCCA AGATATCCTT TATTTCCTGA AAAGGGGAGC 
AGCATTCCAT CCCACTCCTT CCACACGAGT GCCCACCACC AGCCTGTTAA TACTCCTGCC 
TCCGGACCAC TTCCTGTTGG TAACGTAAGC CAGAACCCAA AGATGGCACA GCCAAGAATG 
GAACCAATGT CAAATCCTCA TGTCAAAAGC TATGGCCCAC CAGACAGCCA GCACCCAACC 
CAAGATCGCC TTGGTCAGGA GGTGTGCGGC TCTGGTCATC ACAAAAAAGG TGACCGCAGA 
GCTGATGGAG ACCGCTGTGC TTTGGTGACA GACTCTGCTC CTGAGAGGGA GCTTTCTCCC 
TTACTCTCCT TGCCTTCCCC AGTGCCCCCC CTGTCATCTG TACATTCCAA CCAGCAAGCT 
CTTCCCCGGA CACAAGGAAG CAGCAAGATT CACAGCAGTA ACAGTAATAG TAAAGGCTAT 
TGCCCAGCCA AATCTCCCAA AGACCTAGTA GCAAAGGTCC ATGATAAAGA GACCCCTCAA 
GACAATTTAG TGGCAGTTGC CGGCCTTGGG GTGGCCCCTC CCCAGCCACC TTCTCAGACT 
TTCCCCCCAC CTTCCCTCCC CTCAAAAACT GTTGCGATGC AGCAGAAGCC CACGGCTTAC 
GTCCGGCCCA TGGACGGTCA AGATCAGGCT CCTAGTGAGT CACCTGAACT GAAACCACTG 
CCGGAGGACT ACCGGCAACA GACCTTTGAA AAAACAGACT TAAAAGTGCC TGCCAAAGCC 
AAGCTCACCA AACTGAAGAT GCCATCTCAG TCGGGTGAGC AGACCTACTC CAATGAAGTT 
CATTGTGTTG AAGAGATTTT GAAGGAAATG ACCCATTCAT GGCCACCTCC TTTGACAGCA 
ATACACACGC CTAGTACTGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG 
CACATTAGTT CTGTGACCCA AAATCAAAAA CAATATGATA CATCTTCAAA AACCCACACT 
AATTCTCAGC AGGGAACATC CATGCTTGAA GATGACCTTC AGCTCAGTGA CAGTGAGGAC 
AGTGACAACG AACAAAGCCC AGAGAAGCCT CCTCCTGCGT CTGCGCCTCC CAGGTATTGT 
GTGCATGTTG CCCCGCAGTC GCTTCCAGAA GCAGCGGCCT CAGCACATTC CAGTAGTGCC 
GAGTCAGAGA GCACCAGTGA CTCAGACAGC TCCTCGGACT CCGAGAGCGA GAGCAGCTCA 
AGTGACAGCG AAGAGAATGA GCCCCTAGCA GCTCCGACTC CTGAGCCTGA GCCTCCTACA 
ACAAACAAAT GGCAGCTGGA CAACTGGCTG ACCAAAGTCA GCCAACCGGC AGCCCCACCA 
GACACCCAGG AGGGGAGCGC AGAGCCCCCG CCTCGGCACC CAGACAGTAA GGGCAAGGGG 
GGAGGCGACA GCAGCGGCCC CACCACTGGT CACGAGCTCT CGGAATCCAA AGATCCTCCC 
CTCAAGAGCT CCGGCAAAGC CCCCCGGGGC TCCTCCGAGG GCCCTCACCC CGGCAGGAGG 
AGCTGTCCGA AGTCCCCGGC CCAACAGGAG GCCCTGCCGA GGCAGACTGT GGGAACCAAA 
CAACCCAAGA AGCCGGGCAA GGCCTCCACG CCGGCGGACC CTCGGGCCGG CCTGCAGGTG 
GAGAGCGAGC CGGGCCCCCC TCCGTCCGGC TCCAGAGACC AGCCTTCCAA AGACAAGCCC 
AAGGTGAAGA CGAAAGGGAG GCCGCGCGCC GGCGACAGCA GGGAGCCCAA GCCCGCCGCG 
CCCAGCCCCG GTGACCGCAA GAAGCACCAG AGCGGGCCGC TGGCCCCCTC AAAGGCACTC 
TCGGGCCCCG AGCCCACGAA GGACAATGCG GGGGCCAGGA GCCCCGAGCA CTTTGCCCTG 
GTTCCCCTGA CCCAGAGCCA GGGCCCGCCC CACGGCGGTG GCGGCAGGAC TAGTGGCTGC 
CGGCGCGCAG TGGTGGTCCA GGAGGACCGC AGGAAGGACA AGCTCCCGGG GCCTGCCAGA 
GACACCAAGC TGCTGTCGCC GCTCAGGGAC CCTCCTCCCC CACCCATCTT GATGGTGAAG 
ATAACCCTGG ACCTTCTTTC TCGGATACCC CAGCTTCCTG GGAAAGGGGG CCGGCAGAAG 
AAGCCAGAGG ACAAGCAGCC CCCCACAGGG AAGAAGCCCG ATTTGGAGAA GAGAGGCGCA 
GACAACTCCA GCAAGTTGGT CAAAAAAAGA AAGGCTGAAG CTGAGAAAGA CCATGACAGC 
AAGAAAGCCA GACTGGAGAA AGAAACCAAG TTGCAGTCAT TATCTTCATC CCCCCACAAA 
GAATCTTCCA AAACAAAAAC ACCCAGGCCC TCCTCTGAGC CCTCAAAGAA GGAGATGCTT 
CCCCCCCCAC CTGTGTCATC CTCATCCTCA TCCTCCCAGA AGCCAGCCAA GCCTGCACAA 
AAGAGGCCAA GGCGGGAAGC TGATCCCTCC AGCCAAGACG CTTCCCAAAG TGCCAGTAGT 
AGCAAGGGCA ACCACAAAGA CTCTTCCACT CCCAAGCACA GAAAAGTAGA GGGGAAGGGA 
TCTGGAAGCA GTACAGAGCA CAAGGGATCT TCTGGAGATA GTGCATATCC TTTTCTAGTG 
CCTTCTTTGC CAAATGGTAA CTCTAAACCA GGGAAGCCTC CAGTGAAGCT TGACAGACAA 
CAAGCAGATT TTCACAGGAA GGAGGCCAAA AAGTTGAAGC AAAAAGCAGA GTTAATGATG 
GACAAGGTTG GGAAGGCTTT TAAGTACTTG GAGGCCGCCT TGTCCTTAAT TGAGTGTGGG 
ATTGCCATGG AGTCGGAAAG CCCAGCCTCC AAATCGGCTT ACTCGGTGTA CTCCGAAACC 
GTAGACCTCA TTAAATTTAT AATGTCATTA AAATCCTTCT CAGACGCCTC ATCTCCACAA 
GAGAAAATAT TTGCTGTTTT ATGCATGCGT TGTCAGTCCA TTTTGAACAT GGCGATGTTT 
CGTTGTAAAA AAGATATAGC AATAAAGTAT TCTCGTGCTC TTAACGAACA CTTCAGCAGT 
TTTTTCAAAG TTGCCCAGGC ACCTTCTCCA TGCATTGCAA GAAACACAGG CACACCGTCC 
CCTCTCTCTC CAGTTCCTTC TCCTGCCAGC TCCGTAGGGT CCCAGTCCAG TGCTGGCAGC 
ATAGGGAGCA GTGGTATGCC TGTCACCATC CAAAACATGA CATCTTCTTA TGTCACCATT 
ACTTCCCATG TCCTTACCGC CTTTGACCTC TGGGAACAGG CTGAGGTCCT TACAAGGAAG 
AATAAAGAAT TTTTTGCTCA GCTCAGCACA AGTGTGTGTG CCTTGGCCCT CAACAGCAGT 
TTGATGGATC TGGTGCACTA TACAAGACAG GGTTTTCAGC GGCTAAAACA AGCAACCAAA 
ACACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022269292.1
CDS	67..3735
Translation	MAAQSSLYNEDRNLLRIREKERRNQEAHQEKEAFSEKIPLFGEPYKTEKGDELSSRIQNMLGNYEEVKEFFSSKSHPHRLDASENRLGKPRYPLFPEKGSSIPSHSFHTSAHHQPVNTPASGPLPVGNVSQNPKMAQPRMEPMSNPHVKSYGPPDSQHPTQDRLGQEVCGSGHHKKGDRRADGDRCALVTDSAPERELSPLLSLPSPVPPLSSVHSNQQALPRTQGSSKIHSSNSNSKGYCPAKSPKDLVAKVHDKETPQDNLVAVAGLGVAPPQPPSQTFPPPSLPSKTVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSGEQTYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHISSVTQNQKQYDTSSKTHTNSQQGTSMLEDDLQLSDSEDSDNEQSPEKPPPASAPPRYCVHVAPQSLPEAAASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLAAPTPEPEPPTTNKWQLDNWLTKVSQPAAPPDTQEGSAEPPPRHPDSKGKGGGDSSGPTTGHELSESKDPPLKSSGKAPRGSSEGPHPGRRSCPKSPAQQEALPRQTVGTKQPKKPGKASTPADPRAGLQVESEPGPPPSGSRDQPSKDKPKVKTKGRPRAGDSREPKPAAPSPGDRKKHQSGPLAPSKALSGPEPTKDNAGARSPEHFALVPLTQSQGPPHGGGGRTSGCRRAVVVQEDRRKDKLPGPARDTKLLSPLRDPPPPPILMVKITLDLLSRIPQLPGKGGRQKKPEDKQPPTGKKPDLEKRGADNSSKLVKKRKAEAEKDHDSKKARLEKETKLQSLSSSPHKESSKTKTPRPSSEPSKKEMLPPPPVSSSSSSSQKPAKPAQKRPRREADPSSQDASQSASSSKGNHKDSSTPKHRKVEGKGSGSSTEHKGSSGDSAYPFLVPSLPNGNSKPGKPPVKLDRQQADFHRKEAKKLKQKAELMMDKVGKAFKYLEAALSLIECGIAMESESPASKSAYSVYSETVDLIKFIMSLKSFSDASSPQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRALNEHFSSFFKVAQAPSPCIARNTGTPSPLSPVPSPASSVGSQSSAGSIGSSGMPVTIQNMTSSYVTITSHVLTAFDLWEQAEVLTRKNKEFFAQLSTSVCALALNSSLMDLVHYTRQGFQRLKQATKTP

Target ORF information:

RefSeq Version	XM_022413584.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022413584.1

ORF Insert Sequence:

ATGGCAGCCC AGTCCAGCTT GTACAATGAA GACCGGAACC TGCTTCGAAT TAGAGAGAAG 
GAAAGACGCA ACCAGGAAGC TCACCAAGAG AAAGAGGCAT TTTCTGAAAA GATCCCCCTT 
TTTGGAGAGC CCTACAAGAC AGAAAAAGGT GATGAGCTAT CCAGTCGAAT ACAGAACATG 
TTGGGAAACT ATGAAGAAGT GAAGGAGTTC TTTAGCAGCA AGTCCCACCC TCATCGCTTG 
GATGCCTCTG AAAATAGGTT GGGAAAGCCA AGATATCCTT TATTTCCTGA AAAGGGGAGC 
AGCATTCCAT CCCACTCCTT CCACACGAGT GCCCACCACC AGCCTGTTAA TACTCCTGCC 
TCCGGACCAC TTCCTGTTGG TAACGTAAGC CAGAACCCAA AGATGGCACA GCCAAGAATG 
GAACCAATGT CAAATCCTCA TGTCAAAAGC TATGGCCCAC CAGACAGCCA GCACCCAACC 
CAAGATCGCC TTGGTCAGGA GGTGTGCGGC TCTGGTCATC ACAAAAAAGG TGACCGCAGA 
GCTGATGGAG ACCGCTGTGC TTTGGTGACA GACTCTGCTC CTGAGAGGGA GCTTTCTCCC 
TTACTCTCCT TGCCTTCCCC AGTGCCCCCC CTGTCATCTG TACATTCCAA CCAGCAAGCT 
CTTCCCCGGA CACAAGGAAG CAGCAAGATT CACAGCAGTA ACAGTAATAG TAAAGGCTAT 
TGCCCAGCCA AATCTCCCAA AGACCTAGTA GCAAAGGTCC ATGATAAAGA GACCCCTCAA 
GACAATTTAG TGGCAGTTGC CGGCCTTGGG GTGGCCCCTC CCCAGCCACC TTCTCAGACT 
TTCCCCCCAC CTTCCCTCCC CTCAAAAACT GTTGCGATGC AGCAGAAGCC CACGGCTTAC 
GTCCGGCCCA TGGACGGTCA AGATCAGGCT CCTAGTGAGT CACCTGAACT GAAACCACTG 
CCGGAGGACT ACCGGCAACA GACCTTTGAA AAAACAGACT TAAAAGTGCC TGCCAAAGCC 
AAGCTCACCA AACTGAAGAT GCCATCTCAG TCGGGTGAGC AGACCTACTC CAATGAAGTT 
CATTGTGTTG AAGAGATTTT GAAGGAAATG ACCCATTCAT GGCCACCTCC TTTGACAGCA 
ATACACACGC CTAGTACTGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG 
CACATTAGTT CTGTGACCCA AAATCAAAAA CAATATGATA CATCTTCAAA AACCCACACT 
AATTCTCAGC AGGGAACATC CATGCTTGAA GATGACCTTC AGCTCAGTGA CAGTGAGGAC 
AGTGACAACG AACAAAGCCC AGAGAAGCCT CCTCCTGCGT CTGCGCCTCC CAGGTATTGT 
GTGCATGTTG CCCCGCAGTC GCTTCCAGAA GCAGCGGCCT CAGCACATTC CAGTAGTGCC 
GAGTCAGAGA GCACCAGTGA CTCAGACAGC TCCTCGGACT CCGAGAGCGA GAGCAGCTCA 
AGTGACAGCG AAGAGAATGA GCCCCTAGCA GCTCCGACTC CTGAGCCTGA GCCTCCTACA 
ACAAACAAAT GGCAGCTGGA CAACTGGCTG ACCAAAGTCA GCCAACCGGC AGCCCCACCA 
GACACCCAGG AGGGGAGCGC AGAGCCCCCG CCTCGGCACC CAGACAGTAA GGGCAAGGGG 
GGAGGCGACA GCAGCGGCCC CACCACTGGT CACGAGCTCT CGGAATCCAA AGATCCTCCC 
CTCAAGAGCT CCGGCAAAGC CCCCCGGGGC TCCTCCGAGG GCCCTCACCC CGGCAGGAGG 
AGCTGTCCGA AGTCCCCGGC CCAACAGGAG GCCCTGCCGA GGCAGACTGT GGGAACCAAA 
CAACCCAAGA AGCCGGGCAA GGCCTCCACG CCGGCGGACC CTCGGGCCGG CCTGCAGGTG 
GAGAGCGAGC CGGGCCCCCC TCCGTCCGGC TCCAGAGACC AGCCTTCCAA AGACAAGCCC 
AAGGTGAAGA CGAAAGGGAG GCCGCGCGCC GGCGACAGCA GGGAGCCCAA GCCCGCCGCG 
CCCAGCCCCG GTGACCGCAA GAAGCACCAG AGCGGGCCGC TGGCCCCCTC AAAGGCACTC 
TCGGGCCCCG AGCCCACGAA GGACAATGCG GGGGCCAGGA GCCCCGAGCA CTTTGCCCTG 
GTTCCCCTGA CCCAGAGCCA GGGCCCGCCC CACGGCGGTG GCGGCAGGAC TAGTGGCTGC 
CGGCGCGCAG TGGTGGTCCA GGAGGACCGC AGGAAGGACA AGCTCCCGGG GCCTGCCAGA 
GACACCAAGC TGCTGTCGCC GCTCAGGGAC CCTCCTCCCC CACCCATCTT GATGGTGAAG 
ATAACCCTGG ACCTTCTTTC TCGGATACCC CAGCTTCCTG GGAAAGGGGG CCGGCAGAAG 
AAGCCAGAGG ACAAGCAGCC CCCCACAGGG AAGAAGCCCG ATTTGGAGAA GAGAGGCGCA 
GACAACTCCA GCAAGTTGGT CAAAAAAAGA AAGGCTGAAG CTGAGAAAGA CCATGACAGC 
AAGAAAGCCA GACTGGAGAA AGAAACCAAG TTGCAGTCAT TATCTTCATC CCCCCACAAA 
GAATCTTCCA AAACAAAAAC ACCCAGGCCC TCCTCTGAGC CCTCAAAGAA GGAGATGCTT 
CCCCCCCCAC CTGTGTCATC CTCATCCTCA TCCTCCCAGA AGCCAGCCAA GCCTGCACAA 
AAGAGGCCAA GGCGGGAAGC TGATCCCTCC AGCCAAGACG CTTCCCAAAG TGCCAGTAGT 
AGCAAGGGCA ACCACAAAGA CTCTTCCACT CCCAAGCACA GAAAAGTAGA GGGGAAGGGA 
TCTGGAAGCA GTACAGAGCA CAAGGGATCT TCTGGAGATA GTGCATATCC TTTTCTAGTG 
CCTTCTTTGC CAAATGGTAA CTCTAAACCA GGGAAGCCTC CAGTGAAGCT TGACAGACAA 
CAAGCAGATT TTCACAGGAA GGAGGCCAAA AAGTTGAAGC AAAAAGCAGA GTTAATGATG 
GACAAGGTTG GGAAGGCTTT TAAGTACTTG GAGGCCGCCT TGTCCTTAAT TGAGTGTGGG 
ATTGCCATGG AGTCGGAAAG CCCAGCCTCC AAATCGGCTT ACTCGGTGTA CTCCGAAACC 
GTAGACCTCA TTAAATTTAT AATGTCATTA AAATCCTTCT CAGACGCCTC ATCTCCACAA 
GAGAAAATAT TTGCTGTTTT ATGCATGCGT TGTCAGTCCA TTTTGAACAT GGCGATGTTT 
CGTTGTAAAA AAGATATAGC AATAAAGTAT TCTCGTGCTC TTAACGAACA CTTCAGCAGT 
TTTTTCAAAG TTGCCCAGGC ACCTTCTCCA TGCATTGCAA GAAACACAGG CACACCGTCC 
CCTCTCTCTC CAGTTCCTTC TCCTGCCAGC TCCGTAGGGT CCCAGTCCAG TGCTGGCAGC 
ATAGGGAGCA GTGGTATGCC TGTCACCATC CAAAACATGA CATCTTCTTA TGTCACCATT 
ACTTCCCATG TCCTTACCGC CTTTGACCTC TGGGAACAGG CTGAGGTCCT TACAAGGAAG 
AATAAAGAAT TTTTTGCTCA GCTCAGCACA AGTGTGTGTG CCTTGGCCCT CAACAGCAGT 
TTGATGGATC TGGTGCACTA TACAAGACAG GGTTTTCAGC GGCTAAAACA AGCAACCAAA 
ACACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_022413585.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022269293.1 Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X5, mRNA.

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CloneID	OCe284113
Clone ID Related Accession (Same CDS sequence)	XM_022413585.1
Accession Version	XM_022413585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	AF4/FMR2 family member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGAA ACTATGAAGA AGTGAAGGAG TTCTTTAGCA GCAAGTCCCA CCCTCATCGC 
TTGGATGCCT CTGAAAATAG GTTGGGAAAG CCAAGATATC CTTTATTTCC TGAAAAGGGG 
AGCAGCATTC CATCCCACTC CTTCCACACG AGTGCCCACC ACCAGCCTGT TAATACTCCT 
GCCTCCGGAC CACTTCCTGT TGGTAACGTA AGCCAGAACC CAAAGATGGC ACAGCCAAGA 
ATGGAACCAA TGTCAAATCC TCATGTCAAA AGCTATGGCC CACCAGACAG CCAGCACCCA 
ACCCAAGATC GCCTTGGTCA GGAGGTGTGC GGCTCTGGTC ATCACAAAAA AGGTGACCGC 
AGAGCTGATG GAGACCGCTG TGCTTTGGTG ACAGACTCTG CTCCTGAGAG GGAGCTTTCT 
CCCTTACTCT CCTTGCCTTC CCCAGTGCCC CCCCTGTCAT CTGTACATTC CAACCAGCAA 
GCTCTTCCCC GGACACAAGG AAGCAGCAAG ATTCACAGCA GTAACAGTAA TAGTAAAGGC 
TATTGCCCAG CCAAATCTCC CAAAGACCTA GTAGCAAAGG TCCATGATAA AGAGACCCCT 
CAAGACAATT TAGTGGCAGT TGCCGGCCTT GGGGTGGCCC CTCCCCAGCC ACCTTCTCAG 
ACTTTCCCCC CACCTTCCCT CCCCTCAAAA ACTGTTGCGA TGCAGCAGAA GCCCACGGCT 
TACGTCCGGC CCATGGACGG TCAAGATCAG GCTCCTAGTG AGTCACCTGA ACTGAAACCA 
CTGCCGGAGG ACTACCGGCA ACAGACCTTT GAAAAAACAG ACTTAAAAGT GCCTGCCAAA 
GCCAAGCTCA CCAAACTGAA GATGCCATCT CAGTCGGGTG AGCAGACCTA CTCCAATGAA 
GTTCATTGTG TTGAAGAGAT TTTGAAGGAA ATGACCCATT CATGGCCACC TCCTTTGACA 
GCAATACACA CGCCTAGTAC TGCTGAGCCC TCCAAATTTC CTTTTCCCAC AAAGGATTCT 
CAGCACATTA GTTCTGTGAC CCAAAATCAA AAACAATATG ATACATCTTC AAAAACCCAC 
ACTAATTCTC AGCAGGGAAC ATCCATGCTT GAAGATGACC TTCAGCTCAG TGACAGTGAG 
GACAGTGACA ACGAACAAAG CCCAGAGAAG CCTCCTCCTG CGTCTGCGCC TCCCAGGTAT 
TGTGTGCATG TTGCCCCGCA GTCGCTTCCA GAAGCAGCGG CCTCAGCACA TTCCAGTAGT 
GCCGAGTCAG AGAGCACCAG TGACTCAGAC AGCTCCTCGG ACTCCGAGAG CGAGAGCAGC 
TCAAGTGACA GCGAAGAGAA TGAGCCCCTA GCAGCTCCGA CTCCTGAGCC TGAGCCTCCT 
ACAACAAACA AATGGCAGCT GGACAACTGG CTGACCAAAG TCAGCCAACC GGCAGCCCCA 
CCAGACACCC AGGAGGGGAG CGCAGAGCCC CCGCCTCGGC ACCCAGACAG TAAGGGCAAG 
GGGGGAGGCG ACAGCAGCGG CCCCACCACT GGTCACGAGC TCTCGGAATC CAAAGATCCT 
CCCCTCAAGA GCTCCGGCAA AGCCCCCCGG GGCTCCTCCG AGGGCCCTCA CCCCGGCAGG 
AGGAGCTGTC CGAAGTCCCC GGCCCAACAG GAGGCCCTGC CGAGGCAGAC TGTGGGAACC 
AAACAACCCA AGAAGCCGGG CAAGGCCTCC ACGCCGGCGG ACCCTCGGGC CGGCCTGCAG 
GTGGAGAGCG AGCCGGGCCC CCCTCCGTCC GGCTCCAGAG ACCAGCCTTC CAAAGACAAG 
CCCAAGGTGA AGACGAAAGG GAGGCCGCGC GCCGGCGACA GCAGGGAGCC CAAGCCCGCC 
GCGCCCAGCC CCGGTGACCG CAAGAAGCAC CAGAGCGGGC CGCTGGCCCC CTCAAAGGCA 
CTCTCGGGCC CCGAGCCCAC GAAGGACAAT GCGGGGGCCA GGAGCCCCGA GCACTTTGCC 
CTGGTTCCCC TGACCCAGAG CCAGGGCCCG CCCCACGGCG GTGGCGGCAG GACTAGTGGC 
TGCCGGCGCG CAGTGGTGGT CCAGGAGGAC CGCAGGAAGG ACAAGCTCCC GGGGCCTGCC 
AGAGACACCA AGCTGCTGTC GCCGCTCAGG GACCCTCCTC CCCCACCCAT CTTGATGGTG 
AAGATAACCC TGGACCTTCT TTCTCGGATA CCCCAGCTTC CTGGGAAAGG GGGCCGGCAG 
AAGAAGCCAG AGGACAAGCA GCCCCCCACA GGGAAGAAGC CCGATTTGGA GAAGAGAGGC 
GCAGACAACT CCAGCAAGTT GGTCAAAAAA AGAAAGGCTG AAGCTGAGAA AGACCATGAC 
AGCAAGAAAG CCAGACTGGA GAAAGAAACC AAGTTGCAGT CATTATCTTC ATCCCCCCAC 
AAAGAATCTT CCAAAACAAA AACACCCAGG CCCTCCTCTG AGCCCTCAAA GAAGGAGATG 
CTTCCCCCCC CACCTGTGTC ATCCTCATCC TCATCCTCCC AGAAGCCAGC CAAGCCTGCA 
CAAAAGAGGC CAAGGCGGGA AGCTGATCCC TCCAGCCAAG ACGCTTCCCA AAGTGCCAGT 
AGTAGCAAGG GCAACCACAA AGACTCTTCC ACTCCCAAGC ACAGAAAAGT AGAGGGGAAG 
GGATCTGGAA GCAGTACAGA GCACAAGGGA TCTTCTGGAG ATAGTGCATA TCCTTTTCTA 
GTGCCTTCTT TGCCAAATGG TAACTCTAAA CCAGGGAAGC CTCCAGTGAA GCTTGACAGA 
CAACAAGCAG ATTTTCACAG GAAGGAGGCC AAAAAGTTGA AGCAAAAAGC AGAGTTAATG 
ATGGACAAGG TTGGGAAGGC TTTTAAGTAC TTGGAGGCCG CCTTGTCCTT AATTGAGTGT 
GGGATTGCCA TGGAGTCGGA AAGCCCAGCC TCCAAATCGG CTTACTCGGT GTACTCCGAA 
ACCGTAGACC TCATTAAATT TATAATGTCA TTAAAATCCT TCTCAGACGC CTCATCTCCA 
CAAGAGAAAA TATTTGCTGT TTTATGCATG CGTTGTCAGT CCATTTTGAA CATGGCGATG 
TTTCGTTGTA AAAAAGATAT AGCAATAAAG TATTCTCGTG CTCTTAACGA ACACTTCAGC 
AGTTTTTTCA AAGTTGCCCA GGCACCTTCT CCATGCATTG CAAGAAACAC AGGCACACCG 
TCCCCTCTCT CTCCAGTTCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC CAGTGCTGGC 
AGCATAGGGA GCAGTGGTAT GCCTGTCACC ATCCAAAACA TGACATCTTC TTATGTCACC 
ATTACTTCCC ATGTCCTTAC CGCCTTTGAC CTCTGGGAAC AGGCTGAGGT CCTTACAAGG 
AAGAATAAAG AATTTTTTGC TCAGCTCAGC ACAAGTGTGT GTGCCTTGGC CCTCAACAGC 
AGTTTGATGG ATCTGGTGCA CTATACAAGA CAGGGTTTTC AGCGGCTAAA ACAAGCAACC 
AAAACACCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022269293.1
CDS	184..3675
Translation	MLGNYEEVKEFFSSKSHPHRLDASENRLGKPRYPLFPEKGSSIPSHSFHTSAHHQPVNTPASGPLPVGNVSQNPKMAQPRMEPMSNPHVKSYGPPDSQHPTQDRLGQEVCGSGHHKKGDRRADGDRCALVTDSAPERELSPLLSLPSPVPPLSSVHSNQQALPRTQGSSKIHSSNSNSKGYCPAKSPKDLVAKVHDKETPQDNLVAVAGLGVAPPQPPSQTFPPPSLPSKTVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSGEQTYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHISSVTQNQKQYDTSSKTHTNSQQGTSMLEDDLQLSDSEDSDNEQSPEKPPPASAPPRYCVHVAPQSLPEAAASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLAAPTPEPEPPTTNKWQLDNWLTKVSQPAAPPDTQEGSAEPPPRHPDSKGKGGGDSSGPTTGHELSESKDPPLKSSGKAPRGSSEGPHPGRRSCPKSPAQQEALPRQTVGTKQPKKPGKASTPADPRAGLQVESEPGPPPSGSRDQPSKDKPKVKTKGRPRAGDSREPKPAAPSPGDRKKHQSGPLAPSKALSGPEPTKDNAGARSPEHFALVPLTQSQGPPHGGGGRTSGCRRAVVVQEDRRKDKLPGPARDTKLLSPLRDPPPPPILMVKITLDLLSRIPQLPGKGGRQKKPEDKQPPTGKKPDLEKRGADNSSKLVKKRKAEAEKDHDSKKARLEKETKLQSLSSSPHKESSKTKTPRPSSEPSKKEMLPPPPVSSSSSSSQKPAKPAQKRPRREADPSSQDASQSASSSKGNHKDSSTPKHRKVEGKGSGSSTEHKGSSGDSAYPFLVPSLPNGNSKPGKPPVKLDRQQADFHRKEAKKLKQKAELMMDKVGKAFKYLEAALSLIECGIAMESESPASKSAYSVYSETVDLIKFIMSLKSFSDASSPQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRALNEHFSSFFKVAQAPSPCIARNTGTPSPLSPVPSPASSVGSQSSAGSIGSSGMPVTIQNMTSSYVTITSHVLTAFDLWEQAEVLTRKNKEFFAQLSTSVCALALNSSLMDLVHYTRQGFQRLKQATKTP

Target ORF information:

RefSeq Version	XM_022413585.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022413585.1

ORF Insert Sequence:

ATGTTGGGAA ACTATGAAGA AGTGAAGGAG TTCTTTAGCA GCAAGTCCCA CCCTCATCGC 
TTGGATGCCT CTGAAAATAG GTTGGGAAAG CCAAGATATC CTTTATTTCC TGAAAAGGGG 
AGCAGCATTC CATCCCACTC CTTCCACACG AGTGCCCACC ACCAGCCTGT TAATACTCCT 
GCCTCCGGAC CACTTCCTGT TGGTAACGTA AGCCAGAACC CAAAGATGGC ACAGCCAAGA 
ATGGAACCAA TGTCAAATCC TCATGTCAAA AGCTATGGCC CACCAGACAG CCAGCACCCA 
ACCCAAGATC GCCTTGGTCA GGAGGTGTGC GGCTCTGGTC ATCACAAAAA AGGTGACCGC 
AGAGCTGATG GAGACCGCTG TGCTTTGGTG ACAGACTCTG CTCCTGAGAG GGAGCTTTCT 
CCCTTACTCT CCTTGCCTTC CCCAGTGCCC CCCCTGTCAT CTGTACATTC CAACCAGCAA 
GCTCTTCCCC GGACACAAGG AAGCAGCAAG ATTCACAGCA GTAACAGTAA TAGTAAAGGC 
TATTGCCCAG CCAAATCTCC CAAAGACCTA GTAGCAAAGG TCCATGATAA AGAGACCCCT 
CAAGACAATT TAGTGGCAGT TGCCGGCCTT GGGGTGGCCC CTCCCCAGCC ACCTTCTCAG 
ACTTTCCCCC CACCTTCCCT CCCCTCAAAA ACTGTTGCGA TGCAGCAGAA GCCCACGGCT 
TACGTCCGGC CCATGGACGG TCAAGATCAG GCTCCTAGTG AGTCACCTGA ACTGAAACCA 
CTGCCGGAGG ACTACCGGCA ACAGACCTTT GAAAAAACAG ACTTAAAAGT GCCTGCCAAA 
GCCAAGCTCA CCAAACTGAA GATGCCATCT CAGTCGGGTG AGCAGACCTA CTCCAATGAA 
GTTCATTGTG TTGAAGAGAT TTTGAAGGAA ATGACCCATT CATGGCCACC TCCTTTGACA 
GCAATACACA CGCCTAGTAC TGCTGAGCCC TCCAAATTTC CTTTTCCCAC AAAGGATTCT 
CAGCACATTA GTTCTGTGAC CCAAAATCAA AAACAATATG ATACATCTTC AAAAACCCAC 
ACTAATTCTC AGCAGGGAAC ATCCATGCTT GAAGATGACC TTCAGCTCAG TGACAGTGAG 
GACAGTGACA ACGAACAAAG CCCAGAGAAG CCTCCTCCTG CGTCTGCGCC TCCCAGGTAT 
TGTGTGCATG TTGCCCCGCA GTCGCTTCCA GAAGCAGCGG CCTCAGCACA TTCCAGTAGT 
GCCGAGTCAG AGAGCACCAG TGACTCAGAC AGCTCCTCGG ACTCCGAGAG CGAGAGCAGC 
TCAAGTGACA GCGAAGAGAA TGAGCCCCTA GCAGCTCCGA CTCCTGAGCC TGAGCCTCCT 
ACAACAAACA AATGGCAGCT GGACAACTGG CTGACCAAAG TCAGCCAACC GGCAGCCCCA 
CCAGACACCC AGGAGGGGAG CGCAGAGCCC CCGCCTCGGC ACCCAGACAG TAAGGGCAAG 
GGGGGAGGCG ACAGCAGCGG CCCCACCACT GGTCACGAGC TCTCGGAATC CAAAGATCCT 
CCCCTCAAGA GCTCCGGCAA AGCCCCCCGG GGCTCCTCCG AGGGCCCTCA CCCCGGCAGG 
AGGAGCTGTC CGAAGTCCCC GGCCCAACAG GAGGCCCTGC CGAGGCAGAC TGTGGGAACC 
AAACAACCCA AGAAGCCGGG CAAGGCCTCC ACGCCGGCGG ACCCTCGGGC CGGCCTGCAG 
GTGGAGAGCG AGCCGGGCCC CCCTCCGTCC GGCTCCAGAG ACCAGCCTTC CAAAGACAAG 
CCCAAGGTGA AGACGAAAGG GAGGCCGCGC GCCGGCGACA GCAGGGAGCC CAAGCCCGCC 
GCGCCCAGCC CCGGTGACCG CAAGAAGCAC CAGAGCGGGC CGCTGGCCCC CTCAAAGGCA 
CTCTCGGGCC CCGAGCCCAC GAAGGACAAT GCGGGGGCCA GGAGCCCCGA GCACTTTGCC 
CTGGTTCCCC TGACCCAGAG CCAGGGCCCG CCCCACGGCG GTGGCGGCAG GACTAGTGGC 
TGCCGGCGCG CAGTGGTGGT CCAGGAGGAC CGCAGGAAGG ACAAGCTCCC GGGGCCTGCC 
AGAGACACCA AGCTGCTGTC GCCGCTCAGG GACCCTCCTC CCCCACCCAT CTTGATGGTG 
AAGATAACCC TGGACCTTCT TTCTCGGATA CCCCAGCTTC CTGGGAAAGG GGGCCGGCAG 
AAGAAGCCAG AGGACAAGCA GCCCCCCACA GGGAAGAAGC CCGATTTGGA GAAGAGAGGC 
GCAGACAACT CCAGCAAGTT GGTCAAAAAA AGAAAGGCTG AAGCTGAGAA AGACCATGAC 
AGCAAGAAAG CCAGACTGGA GAAAGAAACC AAGTTGCAGT CATTATCTTC ATCCCCCCAC 
AAAGAATCTT CCAAAACAAA AACACCCAGG CCCTCCTCTG AGCCCTCAAA GAAGGAGATG 
CTTCCCCCCC CACCTGTGTC ATCCTCATCC TCATCCTCCC AGAAGCCAGC CAAGCCTGCA 
CAAAAGAGGC CAAGGCGGGA AGCTGATCCC TCCAGCCAAG ACGCTTCCCA AAGTGCCAGT 
AGTAGCAAGG GCAACCACAA AGACTCTTCC ACTCCCAAGC ACAGAAAAGT AGAGGGGAAG 
GGATCTGGAA GCAGTACAGA GCACAAGGGA TCTTCTGGAG ATAGTGCATA TCCTTTTCTA 
GTGCCTTCTT TGCCAAATGG TAACTCTAAA CCAGGGAAGC CTCCAGTGAA GCTTGACAGA 
CAACAAGCAG ATTTTCACAG GAAGGAGGCC AAAAAGTTGA AGCAAAAAGC AGAGTTAATG 
ATGGACAAGG TTGGGAAGGC TTTTAAGTAC TTGGAGGCCG CCTTGTCCTT AATTGAGTGT 
GGGATTGCCA TGGAGTCGGA AAGCCCAGCC TCCAAATCGG CTTACTCGGT GTACTCCGAA 
ACCGTAGACC TCATTAAATT TATAATGTCA TTAAAATCCT TCTCAGACGC CTCATCTCCA 
CAAGAGAAAA TATTTGCTGT TTTATGCATG CGTTGTCAGT CCATTTTGAA CATGGCGATG 
TTTCGTTGTA AAAAAGATAT AGCAATAAAG TATTCTCGTG CTCTTAACGA ACACTTCAGC 
AGTTTTTTCA AAGTTGCCCA GGCACCTTCT CCATGCATTG CAAGAAACAC AGGCACACCG 
TCCCCTCTCT CTCCAGTTCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC CAGTGCTGGC 
AGCATAGGGA GCAGTGGTAT GCCTGTCACC ATCCAAAACA TGACATCTTC TTATGTCACC 
ATTACTTCCC ATGTCCTTAC CGCCTTTGAC CTCTGGGAAC AGGCTGAGGT CCTTACAAGG 
AAGAATAAAG AATTTTTTGC TCAGCTCAGC ACAAGTGTGT GTGCCTTGGC CCTCAACAGC 
AGTTTGATGG ATCTGGTGCA CTATACAAGA CAGGGTTTTC AGCGGCTAAA ACAAGCAACC 
AAAACACCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_022413582.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022269290.1 Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe29733
Clone ID Related Accession (Same CDS sequence)	XM_022413582.1 , XM_014109957.1 , XM_014109958.1
Accession Version	XM_022413582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	AF4/FMR2 family member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTTA CTGAAAGAGT CCAGAGCAGC AGTAGCAGCA GCTTGTACAA TGAAGACCGG 
AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGAGAAAGAG 
GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCCTACA AGACAGAAAA AGGTGATGAG 
CTATCCAGTC GAATACAGAA CATGTTGGGA AACTATGAAG AAGTGAAGGA GTTCTTTAGC 
AGCAAGTCCC ACCCTCATCG CTTGGATGCC TCTGAAAATA GGTTGGGAAA GCCAAGATAT 
CCTTTATTTC CTGAAAAGGG GAGCAGCATT CCATCCCACT CCTTCCACAC GAGTGCCCAC 
CACCAGCCTG TTAATACTCC TGCCTCCGGA CCACTTCCTG TTGGTAACGT AAGCCAGAAC 
CCAAAGATGG CACAGCCAAG AATGGAACCA ATGTCAAATC CTCATGTCAA AAGCTATGGC 
CCACCAGACA GCCAGCACCC AACCCAAGAT CGCCTTGGTC AGGAGGTGTG CGGCTCTGGT 
CATCACAAAA AAGGTGACCG CAGAGCTGAT GGAGACCGCT GTGCTTTGGT GACAGACTCT 
GCTCCTGAGA GGGAGCTTTC TCCCTTACTC TCCTTGCCTT CCCCAGTGCC CCCCCTGTCA 
TCTGTACATT CCAACCAGCA AGCTCTTCCC CGGACACAAG GAAGCAGCAA GATTCACAGC 
AGTAACAGTA ATAGTAAAGG CTATTGCCCA GCCAAATCTC CCAAAGACCT AGTAGCAAAG 
GTCCATGATA AAGAGACCCC TCAAGACAAT TTAGTGGCAG TTGCCGGCCT TGGGGTGGCC 
CCTCCCCAGC CACCTTCTCA GACTTTCCCC CCACCTTCCC TCCCCTCAAA AACTGTTGCG 
ATGCAGCAGA AGCCCACGGC TTACGTCCGG CCCATGGACG GTCAAGATCA GGCTCCTAGT 
GAGTCACCTG AACTGAAACC ACTGCCGGAG GACTACCGGC AACAGACCTT TGAAAAAACA 
GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACCAAACTGA AGATGCCATC TCAGTCGGGT 
GAGCAGACCT ACTCCAATGA AGTTCATTGT GTTGAAGAGA TTTTGAAGGA AATGACCCAT 
TCATGGCCAC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CTGCTGAGCC CTCCAAATTT 
CCTTTTCCCA CAAAGGATTC TCAGCACATT AGTTCTGTGA CCCAAAATCA AAAACAATAT 
GATACATCTT CAAAAACCCA CACTAATTCT CAGCAGGGAA CATCCATGCT TGAAGATGAC 
CTTCAGCTCA GTGACAGTGA GGACAGTGAC AACGAACAAA GCCCAGAGAA GCCTCCTCCT 
GCGTCTGCGC CTCCCAGTGC CCCGCAGTCG CTTCCAGAAG CAGCGGCCTC AGCACATTCC 
AGTAGTGCCG AGTCAGAGAG CACCAGTGAC TCAGACAGCT CCTCGGACTC CGAGAGCGAG 
AGCAGCTCAA GTGACAGCGA AGAGAATGAG CCCCTAGCAG CTCCGACTCC TGAGCCTGAG 
CCTCCTACAA CAAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAACCGGCA 
GCCCCACCAG ACACCCAGGA GGGGAGCGCA GAGCCCCCGC CTCGGCACCC AGACAGTAAG 
GGCAAGGGGG GAGGCGACAG CAGCGGCCCC ACCACTGGTC ACGAGCTCTC GGAATCCAAA 
GATCCTCCCC TCAAGAGCTC CGGCAAAGCC CCCCGGGGCT CCTCCGAGGG CCCTCACCCC 
GGCAGGAGGA GCTGTCCGAA GTCCCCGGCC CAACAGGAGG CCCTGCCGAG GCAGACTGTG 
GGAACCAAAC AACCCAAGAA GCCGGGCAAG GCCTCCACGC CGGCGGACCC TCGGGCCGGC 
CTGCAGGTGG AGAGCGAGCC GGGCCCCCCT CCGTCCGGCT CCAGAGACCA GCCTTCCAAA 
GACAAGCCCA AGGTGAAGAC GAAAGGGAGG CCGCGCGCCG GCGACAGCAG GGAGCCCAAG 
CCCGCCGCGC CCAGCCCCGG TGACCGCAAG AAGCACCAGA GCGGGCCGCT GGCCCCCTCA 
AAGGCACTCT CGGGCCCCGA GCCCACGAAG GACAATGCGG GGGCCAGGAG CCCCGAGCAC 
TTTGCCCTGG TTCCCCTGAC CCAGAGCCAG GGCCCGCCCC ACGGCGGTGG CGGCAGGACT 
AGTGGCTGCC GGCGCGCAGT GGTGGTCCAG GAGGACCGCA GGAAGGACAA GCTCCCGGGG 
CCTGCCAGAG ACACCAAGCT GCTGTCGCCG CTCAGGGACC CTCCTCCCCC ACCCATCTTG 
ATGGTGAAGA TAACCCTGGA CCTTCTTTCT CGGATACCCC AGCTTCCTGG GAAAGGGGGC 
CGGCAGAAGA AGCCAGAGGA CAAGCAGCCC CCCACAGGGA AGAAGCCCGA TTTGGAGAAG 
AGAGGCGCAG ACAACTCCAG CAAGTTGGTC AAAAAAAGAA AGGCTGAAGC TGAGAAAGAC 
CATGACAGCA AGAAAGCCAG ACTGGAGAAA GAAACCAAGT TGCAGTCATT ATCTTCATCC 
CCCCACAAAG AATCTTCCAA AACAAAAACA CCCAGGCCCT CCTCTGAGCC CTCAAAGAAG 
GAGATGCTTC CCCCCCCACC TGTGTCATCC TCATCCTCAT CCTCCCAGAA GCCAGCCAAG 
CCTGCACAAA AGAGGCCAAG GCGGGAAGCT GATCCCTCCA GCCAAGACGC TTCCCAAAGT 
GCCAGTAGTA GCAAGGGCAA CCACAAAGAC TCTTCCACTC CCAAGCACAG AAAAGTAGAG 
GGGAAGGGAT CTGGAAGCAG TACAGAGCAC AAGGGATCTT CTGGAGATAG TGCATATCCT 
TTTCTAGTGC CTTCTTTGCC AAATGGTAAC TCTAAACCAG GGAAGCCTCC AGTGAAGCTT 
GACAGACAAC AAGCAGATTT TCACAGGAAG GAGGCCAAAA AGTTGAAGCA AAAAGCAGAG 
TTAATGATGG ACAAGGTTGG GAAGGCTTTT AAGTACTTGG AGGCCGCCTT GTCCTTAATT 
GAGTGTGGGA TTGCCATGGA GTCGGAAAGC CCAGCCTCCA AATCGGCTTA CTCGGTGTAC 
TCCGAAACCG TAGACCTCAT TAAATTTATA ATGTCATTAA AATCCTTCTC AGACGCCTCA 
TCTCCACAAG AGAAAATATT TGCTGTTTTA TGCATGCGTT GTCAGTCCAT TTTGAACATG 
GCGATGTTTC GTTGTAAAAA AGATATAGCA ATAAAGTATT CTCGTGCTCT TAACGAACAC 
TTCAGCAGTT TTTTCAAAGT TGCCCAGGCA CCTTCTCCAT GCATTGCAAG AAACACAGGC 
ACACCGTCCC CTCTCTCTCC AGTTCCTTCT CCTGCCAGCT CCGTAGGGTC CCAGTCCAGT 
GCTGGCAGCA TAGGGAGCAG TGGTATGCCT GTCACCATCC AAAACATGAC ATCTTCTTAT 
GTCACCATTA CTTCCCATGT CCTTACCGCC TTTGACCTCT GGGAACAGGC TGAGGTCCTT 
ACAAGGAAGA ATAAAGAATT TTTTGCTCAG CTCAGCACAA GTGTGTGTGC CTTGGCCCTC 
AACAGCAGTT TGATGGATCT GGTGCACTAT ACAAGACAGG GTTTTCAGCG GCTAAAACAA 
GCAACCAAAA CACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022269290.1
CDS	278..3955
Translation	MAFTERVQSSSSSSLYNEDRNLLRIREKERRNQEAHQEKEAFSEKIPLFGEPYKTEKGDELSSRIQNMLGNYEEVKEFFSSKSHPHRLDASENRLGKPRYPLFPEKGSSIPSHSFHTSAHHQPVNTPASGPLPVGNVSQNPKMAQPRMEPMSNPHVKSYGPPDSQHPTQDRLGQEVCGSGHHKKGDRRADGDRCALVTDSAPERELSPLLSLPSPVPPLSSVHSNQQALPRTQGSSKIHSSNSNSKGYCPAKSPKDLVAKVHDKETPQDNLVAVAGLGVAPPQPPSQTFPPPSLPSKTVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSGEQTYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHISSVTQNQKQYDTSSKTHTNSQQGTSMLEDDLQLSDSEDSDNEQSPEKPPPASAPPSAPQSLPEAAASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLAAPTPEPEPPTTNKWQLDNWLTKVSQPAAPPDTQEGSAEPPPRHPDSKGKGGGDSSGPTTGHELSESKDPPLKSSGKAPRGSSEGPHPGRRSCPKSPAQQEALPRQTVGTKQPKKPGKASTPADPRAGLQVESEPGPPPSGSRDQPSKDKPKVKTKGRPRAGDSREPKPAAPSPGDRKKHQSGPLAPSKALSGPEPTKDNAGARSPEHFALVPLTQSQGPPHGGGGRTSGCRRAVVVQEDRRKDKLPGPARDTKLLSPLRDPPPPPILMVKITLDLLSRIPQLPGKGGRQKKPEDKQPPTGKKPDLEKRGADNSSKLVKKRKAEAEKDHDSKKARLEKETKLQSLSSSPHKESSKTKTPRPSSEPSKKEMLPPPPVSSSSSSSQKPAKPAQKRPRREADPSSQDASQSASSSKGNHKDSSTPKHRKVEGKGSGSSTEHKGSSGDSAYPFLVPSLPNGNSKPGKPPVKLDRQQADFHRKEAKKLKQKAELMMDKVGKAFKYLEAALSLIECGIAMESESPASKSAYSVYSETVDLIKFIMSLKSFSDASSPQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRALNEHFSSFFKVAQAPSPCIARNTGTPSPLSPVPSPASSVGSQSSAGSIGSSGMPVTIQNMTSSYVTITSHVLTAFDLWEQAEVLTRKNKEFFAQLSTSVCALALNSSLMDLVHYTRQGFQRLKQATKTP

Target ORF information:

RefSeq Version	XM_022413582.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022413582.1

ORF Insert Sequence:

ATGGCGTTTA CTGAAAGAGT CCAGAGCAGC AGTAGCAGCA GCTTGTACAA TGAAGACCGG 
AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGAGAAAGAG 
GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCCTACA AGACAGAAAA AGGTGATGAG 
CTATCCAGTC GAATACAGAA CATGTTGGGA AACTATGAAG AAGTGAAGGA GTTCTTTAGC 
AGCAAGTCCC ACCCTCATCG CTTGGATGCC TCTGAAAATA GGTTGGGAAA GCCAAGATAT 
CCTTTATTTC CTGAAAAGGG GAGCAGCATT CCATCCCACT CCTTCCACAC GAGTGCCCAC 
CACCAGCCTG TTAATACTCC TGCCTCCGGA CCACTTCCTG TTGGTAACGT AAGCCAGAAC 
CCAAAGATGG CACAGCCAAG AATGGAACCA ATGTCAAATC CTCATGTCAA AAGCTATGGC 
CCACCAGACA GCCAGCACCC AACCCAAGAT CGCCTTGGTC AGGAGGTGTG CGGCTCTGGT 
CATCACAAAA AAGGTGACCG CAGAGCTGAT GGAGACCGCT GTGCTTTGGT GACAGACTCT 
GCTCCTGAGA GGGAGCTTTC TCCCTTACTC TCCTTGCCTT CCCCAGTGCC CCCCCTGTCA 
TCTGTACATT CCAACCAGCA AGCTCTTCCC CGGACACAAG GAAGCAGCAA GATTCACAGC 
AGTAACAGTA ATAGTAAAGG CTATTGCCCA GCCAAATCTC CCAAAGACCT AGTAGCAAAG 
GTCCATGATA AAGAGACCCC TCAAGACAAT TTAGTGGCAG TTGCCGGCCT TGGGGTGGCC 
CCTCCCCAGC CACCTTCTCA GACTTTCCCC CCACCTTCCC TCCCCTCAAA AACTGTTGCG 
ATGCAGCAGA AGCCCACGGC TTACGTCCGG CCCATGGACG GTCAAGATCA GGCTCCTAGT 
GAGTCACCTG AACTGAAACC ACTGCCGGAG GACTACCGGC AACAGACCTT TGAAAAAACA 
GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACCAAACTGA AGATGCCATC TCAGTCGGGT 
GAGCAGACCT ACTCCAATGA AGTTCATTGT GTTGAAGAGA TTTTGAAGGA AATGACCCAT 
TCATGGCCAC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CTGCTGAGCC CTCCAAATTT 
CCTTTTCCCA CAAAGGATTC TCAGCACATT AGTTCTGTGA CCCAAAATCA AAAACAATAT 
GATACATCTT CAAAAACCCA CACTAATTCT CAGCAGGGAA CATCCATGCT TGAAGATGAC 
CTTCAGCTCA GTGACAGTGA GGACAGTGAC AACGAACAAA GCCCAGAGAA GCCTCCTCCT 
GCGTCTGCGC CTCCCAGTGC CCCGCAGTCG CTTCCAGAAG CAGCGGCCTC AGCACATTCC 
AGTAGTGCCG AGTCAGAGAG CACCAGTGAC TCAGACAGCT CCTCGGACTC CGAGAGCGAG 
AGCAGCTCAA GTGACAGCGA AGAGAATGAG CCCCTAGCAG CTCCGACTCC TGAGCCTGAG 
CCTCCTACAA CAAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAACCGGCA 
GCCCCACCAG ACACCCAGGA GGGGAGCGCA GAGCCCCCGC CTCGGCACCC AGACAGTAAG 
GGCAAGGGGG GAGGCGACAG CAGCGGCCCC ACCACTGGTC ACGAGCTCTC GGAATCCAAA 
GATCCTCCCC TCAAGAGCTC CGGCAAAGCC CCCCGGGGCT CCTCCGAGGG CCCTCACCCC 
GGCAGGAGGA GCTGTCCGAA GTCCCCGGCC CAACAGGAGG CCCTGCCGAG GCAGACTGTG 
GGAACCAAAC AACCCAAGAA GCCGGGCAAG GCCTCCACGC CGGCGGACCC TCGGGCCGGC 
CTGCAGGTGG AGAGCGAGCC GGGCCCCCCT CCGTCCGGCT CCAGAGACCA GCCTTCCAAA 
GACAAGCCCA AGGTGAAGAC GAAAGGGAGG CCGCGCGCCG GCGACAGCAG GGAGCCCAAG 
CCCGCCGCGC CCAGCCCCGG TGACCGCAAG AAGCACCAGA GCGGGCCGCT GGCCCCCTCA 
AAGGCACTCT CGGGCCCCGA GCCCACGAAG GACAATGCGG GGGCCAGGAG CCCCGAGCAC 
TTTGCCCTGG TTCCCCTGAC CCAGAGCCAG GGCCCGCCCC ACGGCGGTGG CGGCAGGACT 
AGTGGCTGCC GGCGCGCAGT GGTGGTCCAG GAGGACCGCA GGAAGGACAA GCTCCCGGGG 
CCTGCCAGAG ACACCAAGCT GCTGTCGCCG CTCAGGGACC CTCCTCCCCC ACCCATCTTG 
ATGGTGAAGA TAACCCTGGA CCTTCTTTCT CGGATACCCC AGCTTCCTGG GAAAGGGGGC 
CGGCAGAAGA AGCCAGAGGA CAAGCAGCCC CCCACAGGGA AGAAGCCCGA TTTGGAGAAG 
AGAGGCGCAG ACAACTCCAG CAAGTTGGTC AAAAAAAGAA AGGCTGAAGC TGAGAAAGAC 
CATGACAGCA AGAAAGCCAG ACTGGAGAAA GAAACCAAGT TGCAGTCATT ATCTTCATCC 
CCCCACAAAG AATCTTCCAA AACAAAAACA CCCAGGCCCT CCTCTGAGCC CTCAAAGAAG 
GAGATGCTTC CCCCCCCACC TGTGTCATCC TCATCCTCAT CCTCCCAGAA GCCAGCCAAG 
CCTGCACAAA AGAGGCCAAG GCGGGAAGCT GATCCCTCCA GCCAAGACGC TTCCCAAAGT 
GCCAGTAGTA GCAAGGGCAA CCACAAAGAC TCTTCCACTC CCAAGCACAG AAAAGTAGAG 
GGGAAGGGAT CTGGAAGCAG TACAGAGCAC AAGGGATCTT CTGGAGATAG TGCATATCCT 
TTTCTAGTGC CTTCTTTGCC AAATGGTAAC TCTAAACCAG GGAAGCCTCC AGTGAAGCTT 
GACAGACAAC AAGCAGATTT TCACAGGAAG GAGGCCAAAA AGTTGAAGCA AAAAGCAGAG 
TTAATGATGG ACAAGGTTGG GAAGGCTTTT AAGTACTTGG AGGCCGCCTT GTCCTTAATT 
GAGTGTGGGA TTGCCATGGA GTCGGAAAGC CCAGCCTCCA AATCGGCTTA CTCGGTGTAC 
TCCGAAACCG TAGACCTCAT TAAATTTATA ATGTCATTAA AATCCTTCTC AGACGCCTCA 
TCTCCACAAG AGAAAATATT TGCTGTTTTA TGCATGCGTT GTCAGTCCAT TTTGAACATG 
GCGATGTTTC GTTGTAAAAA AGATATAGCA ATAAAGTATT CTCGTGCTCT TAACGAACAC 
TTCAGCAGTT TTTTCAAAGT TGCCCAGGCA CCTTCTCCAT GCATTGCAAG AAACACAGGC 
ACACCGTCCC CTCTCTCTCC AGTTCCTTCT CCTGCCAGCT CCGTAGGGTC CCAGTCCAGT 
GCTGGCAGCA TAGGGAGCAG TGGTATGCCT GTCACCATCC AAAACATGAC ATCTTCTTAT 
GTCACCATTA CTTCCCATGT CCTTACCGCC TTTGACCTCT GGGAACAGGC TGAGGTCCTT 
ACAAGGAAGA ATAAAGAATT TTTTGCTCAG CTCAGCACAA GTGTGTGTGC CTTGGCCCTC 
AACAGCAGTT TGATGGATCT GGTGCACTAT ACAAGACAGG GTTTTCAGCG GCTAAAACAA 
GCAACCAAAA CACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_014109957.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965432.1 Canis lupus familiaris AF4/FMR2 family, member 1 (AFF1), transcript variant X1, mRNA.

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CloneID	OCe29733
Clone ID Related Accession (Same CDS sequence)	XM_022413582.1 , XM_014109957.1 , XM_014109958.1
Accession Version	XM_014109957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	AF4/FMR2 family member 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTTA CTGAAAGAGT CCAGAGCAGC AGTAGCAGCA GCTTGTACAA TGAAGACCGG 
AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGAGAAAGAG 
GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCCTACA AGACAGAAAA AGGTGATGAG 
CTATCCAGTC GAATACAGAA CATGTTGGGA AACTATGAAG AAGTGAAGGA GTTCTTTAGC 
AGCAAGTCCC ACCCTCATCG CTTGGATGCC TCTGAAAATA GGTTGGGAAA GCCAAGATAT 
CCTTTATTTC CTGAAAAGGG GAGCAGCATT CCATCCCACT CCTTCCACAC GAGTGCCCAC 
CACCAGCCTG TTAATACTCC TGCCTCCGGA CCACTTCCTG TTGGTAACGT AAGCCAGAAC 
CCAAAGATGG CACAGCCAAG AATGGAACCA ATGTCAAATC CTCATGTCAA AAGCTATGGC 
CCACCAGACA GCCAGCACCC AACCCAAGAT CGCCTTGGTC AGGAGGTGTG CGGCTCTGGT 
CATCACAAAA AAGGTGACCG CAGAGCTGAT GGAGACCGCT GTGCTTTGGT GACAGACTCT 
GCTCCTGAGA GGGAGCTTTC TCCCTTACTC TCCTTGCCTT CCCCAGTGCC CCCCCTGTCA 
TCTGTACATT CCAACCAGCA AGCTCTTCCC CGGACACAAG GAAGCAGCAA GATTCACAGC 
AGTAACAGTA ATAGTAAAGG CTATTGCCCA GCCAAATCTC CCAAAGACCT AGTAGCAAAG 
GTCCATGATA AAGAGACCCC TCAAGACAAT TTAGTGGCAG TTGCCGGCCT TGGGGTGGCC 
CCTCCCCAGC CACCTTCTCA GACTTTCCCC CCACCTTCCC TCCCCTCAAA AACTGTTGCG 
ATGCAGCAGA AGCCCACGGC TTACGTCCGG CCCATGGACG GTCAAGATCA GGCTCCTAGT 
GAGTCACCTG AACTGAAACC ACTGCCGGAG GACTACCGGC AACAGACCTT TGAAAAAACA 
GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACCAAACTGA AGATGCCATC TCAGTCGGGT 
GAGCAGACCT ACTCCAATGA AGTTCATTGT GTTGAAGAGA TTTTGAAGGA AATGACCCAT 
TCATGGCCAC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CTGCTGAGCC CTCCAAATTT 
CCTTTTCCCA CAAAGGATTC TCAGCACATT AGTTCTGTGA CCCAAAATCA AAAACAATAT 
GATACATCTT CAAAAACCCA CACTAATTCT CAGCAGGGAA CATCCATGCT TGAAGATGAC 
CTTCAGCTCA GTGACAGTGA GGACAGTGAC AACGAACAAA GCCCAGAGAA GCCTCCTCCT 
GCGTCTGCGC CTCCCAGTGC CCCGCAGTCG CTTCCAGAAG CAGCGGCCTC AGCACATTCC 
AGTAGTGCCG AGTCAGAGAG CACCAGTGAC TCAGACAGCT CCTCGGACTC CGAGAGCGAG 
AGCAGCTCAA GTGACAGCGA AGAGAATGAG CCCCTAGCAG CTCCGACTCC TGAGCCTGAG 
CCTCCTACAA CAAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAACCGGCA 
GCCCCACCAG ACACCCAGGA GGGGAGCGCA GAGCCCCCGC CTCGGCACCC AGACAGTAAG 
GGCAAGGGGG GAGGCGACAG CAGCGGCCCC ACCACTGGTC ACGAGCTCTC GGAATCCAAA 
GATCCTCCCC TCAAGAGCTC CGGCAAAGCC CCCCGGGGCT CCTCCGAGGG CCCTCACCCC 
GGCAGGAGGA GCTGTCCGAA GTCCCCGGCC CAACAGGAGG CCCTGCCGAG GCAGACTGTG 
GGAACCAAAC AACCCAAGAA GCCGGGCAAG GCCTCCACGC CGGCGGACCC TCGGGCCGGC 
CTGCAGGTGG AGAGCGAGCC GGGCCCCCCT CCGTCCGGCT CCAGAGACCA GCCTTCCAAA 
GACAAGCCCA AGGTGAAGAC GAAAGGGAGG CCGCGCGCCG GCGACAGCAG GGAGCCCAAG 
CCCGCCGCGC CCAGCCCCGG TGACCGCAAG AAGCACCAGA GCGGGCCGCT GGCCCCCTCA 
AAGGCACTCT CGGGCCCCGA GCCCACGAAG GACAATGCGG GGGCCAGGAG CCCCGAGCAC 
TTTGCCCTGG TTCCCCTGAC CCAGAGCCAG GGCCCGCCCC ACGGCGGTGG CGGCAGGACT 
AGTGGCTGCC GGCGCGCAGT GGTGGTCCAG GAGGACCGCA GGAAGGACAA GCTCCCGGGG 
CCTGCCAGAG ACACCAAGCT GCTGTCGCCG CTCAGGGACC CTCCTCCCCC ACCCATCTTG 
ATGGTGAAGA TAACCCTGGA CCTTCTTTCT CGGATACCCC AGCTTCCTGG GAAAGGGGGC 
CGGCAGAAGA AGCCAGAGGA CAAGCAGCCC CCCACAGGGA AGAAGCCCGA TTTGGAGAAG 
AGAGGCGCAG ACAACTCCAG CAAGTTGGTC AAAAAAAGAA AGGCTGAAGC TGAGAAAGAC 
CATGACAGCA AGAAAGCCAG ACTGGAGAAA GAAACCAAGT TGCAGTCATT ATCTTCATCC 
CCCCACAAAG AATCTTCCAA AACAAAAACA CCCAGGCCCT CCTCTGAGCC CTCAAAGAAG 
GAGATGCTTC CCCCCCCACC TGTGTCATCC TCATCCTCAT CCTCCCAGAA GCCAGCCAAG 
CCTGCACAAA AGAGGCCAAG GCGGGAAGCT GATCCCTCCA GCCAAGACGC TTCCCAAAGT 
GCCAGTAGTA GCAAGGGCAA CCACAAAGAC TCTTCCACTC CCAAGCACAG AAAAGTAGAG 
GGGAAGGGAT CTGGAAGCAG TACAGAGCAC AAGGGATCTT CTGGAGATAG TGCATATCCT 
TTTCTAGTGC CTTCTTTGCC AAATGGTAAC TCTAAACCAG GGAAGCCTCC AGTGAAGCTT 
GACAGACAAC AAGCAGATTT TCACAGGAAG GAGGCCAAAA AGTTGAAGCA AAAAGCAGAG 
TTAATGATGG ACAAGGTTGG GAAGGCTTTT AAGTACTTGG AGGCCGCCTT GTCCTTAATT 
GAGTGTGGGA TTGCCATGGA GTCGGAAAGC CCAGCCTCCA AATCGGCTTA CTCGGTGTAC 
TCCGAAACCG TAGACCTCAT TAAATTTATA ATGTCATTAA AATCCTTCTC AGACGCCTCA 
TCTCCACAAG AGAAAATATT TGCTGTTTTA TGCATGCGTT GTCAGTCCAT TTTGAACATG 
GCGATGTTTC GTTGTAAAAA AGATATAGCA ATAAAGTATT CTCGTGCTCT TAACGAACAC 
TTCAGCAGTT TTTTCAAAGT TGCCCAGGCA CCTTCTCCAT GCATTGCAAG AAACACAGGC 
ACACCGTCCC CTCTCTCTCC AGTTCCTTCT CCTGCCAGCT CCGTAGGGTC CCAGTCCAGT 
GCTGGCAGCA TAGGGAGCAG TGGTATGCCT GTCACCATCC AAAACATGAC ATCTTCTTAT 
GTCACCATTA CTTCCCATGT CCTTACCGCC TTTGACCTCT GGGAACAGGC TGAGGTCCTT 
ACAAGGAAGA ATAAAGAATT TTTTGCTCAG CTCAGCACAA GTGTGTGTGC CTTGGCCCTC 
AACAGCAGTT TGATGGATCT GGTGCACTAT ACAAGACAGG GTTTTCAGCG GCTAAAACAA 
GCAACCAAAA CACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965432.1
CDS	316..3993
Translation	MAFTERVQSSSSSSLYNEDRNLLRIREKERRNQEAHQEKEAFSEKIPLFGEPYKTEKGDELSSRIQNMLGNYEEVKEFFSSKSHPHRLDASENRLGKPRYPLFPEKGSSIPSHSFHTSAHHQPVNTPASGPLPVGNVSQNPKMAQPRMEPMSNPHVKSYGPPDSQHPTQDRLGQEVCGSGHHKKGDRRADGDRCALVTDSAPERELSPLLSLPSPVPPLSSVHSNQQALPRTQGSSKIHSSNSNSKGYCPAKSPKDLVAKVHDKETPQDNLVAVAGLGVAPPQPPSQTFPPPSLPSKTVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSGEQTYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHISSVTQNQKQYDTSSKTHTNSQQGTSMLEDDLQLSDSEDSDNEQSPEKPPPASAPPSAPQSLPEAAASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLAAPTPEPEPPTTNKWQLDNWLTKVSQPAAPPDTQEGSAEPPPRHPDSKGKGGGDSSGPTTGHELSESKDPPLKSSGKAPRGSSEGPHPGRRSCPKSPAQQEALPRQTVGTKQPKKPGKASTPADPRAGLQVESEPGPPPSGSRDQPSKDKPKVKTKGRPRAGDSREPKPAAPSPGDRKKHQSGPLAPSKALSGPEPTKDNAGARSPEHFALVPLTQSQGPPHGGGGRTSGCRRAVVVQEDRRKDKLPGPARDTKLLSPLRDPPPPPILMVKITLDLLSRIPQLPGKGGRQKKPEDKQPPTGKKPDLEKRGADNSSKLVKKRKAEAEKDHDSKKARLEKETKLQSLSSSPHKESSKTKTPRPSSEPSKKEMLPPPPVSSSSSSSQKPAKPAQKRPRREADPSSQDASQSASSSKGNHKDSSTPKHRKVEGKGSGSSTEHKGSSGDSAYPFLVPSLPNGNSKPGKPPVKLDRQQADFHRKEAKKLKQKAELMMDKVGKAFKYLEAALSLIECGIAMESESPASKSAYSVYSETVDLIKFIMSLKSFSDASSPQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRALNEHFSSFFKVAQAPSPCIARNTGTPSPLSPVPSPASSVGSQSSAGSIGSSGMPVTIQNMTSSYVTITSHVLTAFDLWEQAEVLTRKNKEFFAQLSTSVCALALNSSLMDLVHYTRQGFQRLKQATKTP

Target ORF information:

RefSeq Version	XM_014109957.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris AF4/FMR2 family, member 1 (AFF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109957.1

ORF Insert Sequence:

ATGGCGTTTA CTGAAAGAGT CCAGAGCAGC AGTAGCAGCA GCTTGTACAA TGAAGACCGG 
AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGAGAAAGAG 
GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCCTACA AGACAGAAAA AGGTGATGAG 
CTATCCAGTC GAATACAGAA CATGTTGGGA AACTATGAAG AAGTGAAGGA GTTCTTTAGC 
AGCAAGTCCC ACCCTCATCG CTTGGATGCC TCTGAAAATA GGTTGGGAAA GCCAAGATAT 
CCTTTATTTC CTGAAAAGGG GAGCAGCATT CCATCCCACT CCTTCCACAC GAGTGCCCAC 
CACCAGCCTG TTAATACTCC TGCCTCCGGA CCACTTCCTG TTGGTAACGT AAGCCAGAAC 
CCAAAGATGG CACAGCCAAG AATGGAACCA ATGTCAAATC CTCATGTCAA AAGCTATGGC 
CCACCAGACA GCCAGCACCC AACCCAAGAT CGCCTTGGTC AGGAGGTGTG CGGCTCTGGT 
CATCACAAAA AAGGTGACCG CAGAGCTGAT GGAGACCGCT GTGCTTTGGT GACAGACTCT 
GCTCCTGAGA GGGAGCTTTC TCCCTTACTC TCCTTGCCTT CCCCAGTGCC CCCCCTGTCA 
TCTGTACATT CCAACCAGCA AGCTCTTCCC CGGACACAAG GAAGCAGCAA GATTCACAGC 
AGTAACAGTA ATAGTAAAGG CTATTGCCCA GCCAAATCTC CCAAAGACCT AGTAGCAAAG 
GTCCATGATA AAGAGACCCC TCAAGACAAT TTAGTGGCAG TTGCCGGCCT TGGGGTGGCC 
CCTCCCCAGC CACCTTCTCA GACTTTCCCC CCACCTTCCC TCCCCTCAAA AACTGTTGCG 
ATGCAGCAGA AGCCCACGGC TTACGTCCGG CCCATGGACG GTCAAGATCA GGCTCCTAGT 
GAGTCACCTG AACTGAAACC ACTGCCGGAG GACTACCGGC AACAGACCTT TGAAAAAACA 
GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACCAAACTGA AGATGCCATC TCAGTCGGGT 
GAGCAGACCT ACTCCAATGA AGTTCATTGT GTTGAAGAGA TTTTGAAGGA AATGACCCAT 
TCATGGCCAC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CTGCTGAGCC CTCCAAATTT 
CCTTTTCCCA CAAAGGATTC TCAGCACATT AGTTCTGTGA CCCAAAATCA AAAACAATAT 
GATACATCTT CAAAAACCCA CACTAATTCT CAGCAGGGAA CATCCATGCT TGAAGATGAC 
CTTCAGCTCA GTGACAGTGA GGACAGTGAC AACGAACAAA GCCCAGAGAA GCCTCCTCCT 
GCGTCTGCGC CTCCCAGTGC CCCGCAGTCG CTTCCAGAAG CAGCGGCCTC AGCACATTCC 
AGTAGTGCCG AGTCAGAGAG CACCAGTGAC TCAGACAGCT CCTCGGACTC CGAGAGCGAG 
AGCAGCTCAA GTGACAGCGA AGAGAATGAG CCCCTAGCAG CTCCGACTCC TGAGCCTGAG 
CCTCCTACAA CAAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAACCGGCA 
GCCCCACCAG ACACCCAGGA GGGGAGCGCA GAGCCCCCGC CTCGGCACCC AGACAGTAAG 
GGCAAGGGGG GAGGCGACAG CAGCGGCCCC ACCACTGGTC ACGAGCTCTC GGAATCCAAA 
GATCCTCCCC TCAAGAGCTC CGGCAAAGCC CCCCGGGGCT CCTCCGAGGG CCCTCACCCC 
GGCAGGAGGA GCTGTCCGAA GTCCCCGGCC CAACAGGAGG CCCTGCCGAG GCAGACTGTG 
GGAACCAAAC AACCCAAGAA GCCGGGCAAG GCCTCCACGC CGGCGGACCC TCGGGCCGGC 
CTGCAGGTGG AGAGCGAGCC GGGCCCCCCT CCGTCCGGCT CCAGAGACCA GCCTTCCAAA 
GACAAGCCCA AGGTGAAGAC GAAAGGGAGG CCGCGCGCCG GCGACAGCAG GGAGCCCAAG 
CCCGCCGCGC CCAGCCCCGG TGACCGCAAG AAGCACCAGA GCGGGCCGCT GGCCCCCTCA 
AAGGCACTCT CGGGCCCCGA GCCCACGAAG GACAATGCGG GGGCCAGGAG CCCCGAGCAC 
TTTGCCCTGG TTCCCCTGAC CCAGAGCCAG GGCCCGCCCC ACGGCGGTGG CGGCAGGACT 
AGTGGCTGCC GGCGCGCAGT GGTGGTCCAG GAGGACCGCA GGAAGGACAA GCTCCCGGGG 
CCTGCCAGAG ACACCAAGCT GCTGTCGCCG CTCAGGGACC CTCCTCCCCC ACCCATCTTG 
ATGGTGAAGA TAACCCTGGA CCTTCTTTCT CGGATACCCC AGCTTCCTGG GAAAGGGGGC 
CGGCAGAAGA AGCCAGAGGA CAAGCAGCCC CCCACAGGGA AGAAGCCCGA TTTGGAGAAG 
AGAGGCGCAG ACAACTCCAG CAAGTTGGTC AAAAAAAGAA AGGCTGAAGC TGAGAAAGAC 
CATGACAGCA AGAAAGCCAG ACTGGAGAAA GAAACCAAGT TGCAGTCATT ATCTTCATCC 
CCCCACAAAG AATCTTCCAA AACAAAAACA CCCAGGCCCT CCTCTGAGCC CTCAAAGAAG 
GAGATGCTTC CCCCCCCACC TGTGTCATCC TCATCCTCAT CCTCCCAGAA GCCAGCCAAG 
CCTGCACAAA AGAGGCCAAG GCGGGAAGCT GATCCCTCCA GCCAAGACGC TTCCCAAAGT 
GCCAGTAGTA GCAAGGGCAA CCACAAAGAC TCTTCCACTC CCAAGCACAG AAAAGTAGAG 
GGGAAGGGAT CTGGAAGCAG TACAGAGCAC AAGGGATCTT CTGGAGATAG TGCATATCCT 
TTTCTAGTGC CTTCTTTGCC AAATGGTAAC TCTAAACCAG GGAAGCCTCC AGTGAAGCTT 
GACAGACAAC AAGCAGATTT TCACAGGAAG GAGGCCAAAA AGTTGAAGCA AAAAGCAGAG 
TTAATGATGG ACAAGGTTGG GAAGGCTTTT AAGTACTTGG AGGCCGCCTT GTCCTTAATT 
GAGTGTGGGA TTGCCATGGA GTCGGAAAGC CCAGCCTCCA AATCGGCTTA CTCGGTGTAC 
TCCGAAACCG TAGACCTCAT TAAATTTATA ATGTCATTAA AATCCTTCTC AGACGCCTCA 
TCTCCACAAG AGAAAATATT TGCTGTTTTA TGCATGCGTT GTCAGTCCAT TTTGAACATG 
GCGATGTTTC GTTGTAAAAA AGATATAGCA ATAAAGTATT CTCGTGCTCT TAACGAACAC 
TTCAGCAGTT TTTTCAAAGT TGCCCAGGCA CCTTCTCCAT GCATTGCAAG AAACACAGGC 
ACACCGTCCC CTCTCTCTCC AGTTCCTTCT CCTGCCAGCT CCGTAGGGTC CCAGTCCAGT 
GCTGGCAGCA TAGGGAGCAG TGGTATGCCT GTCACCATCC AAAACATGAC ATCTTCTTAT 
GTCACCATTA CTTCCCATGT CCTTACCGCC TTTGACCTCT GGGAACAGGC TGAGGTCCTT 
ACAAGGAAGA ATAAAGAATT TTTTGCTCAG CTCAGCACAA GTGTGTGTGC CTTGGCCCTC 
AACAGCAGTT TGATGGATCT GGTGCACTAT ACAAGACAGG GTTTTCAGCG GCTAAAACAA 
GCAACCAAAA CACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_014109958.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965433.1 Canis lupus familiaris AF4/FMR2 family, member 1 (AFF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe29733
Clone ID Related Accession (Same CDS sequence)	XM_022413582.1 , XM_014109957.1 , XM_014109958.1
Accession Version	XM_014109958.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	AF4/FMR2 family member 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTTA CTGAAAGAGT CCAGAGCAGC AGTAGCAGCA GCTTGTACAA TGAAGACCGG 
AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGAGAAAGAG 
GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCCTACA AGACAGAAAA AGGTGATGAG 
CTATCCAGTC GAATACAGAA CATGTTGGGA AACTATGAAG AAGTGAAGGA GTTCTTTAGC 
AGCAAGTCCC ACCCTCATCG CTTGGATGCC TCTGAAAATA GGTTGGGAAA GCCAAGATAT 
CCTTTATTTC CTGAAAAGGG GAGCAGCATT CCATCCCACT CCTTCCACAC GAGTGCCCAC 
CACCAGCCTG TTAATACTCC TGCCTCCGGA CCACTTCCTG TTGGTAACGT AAGCCAGAAC 
CCAAAGATGG CACAGCCAAG AATGGAACCA ATGTCAAATC CTCATGTCAA AAGCTATGGC 
CCACCAGACA GCCAGCACCC AACCCAAGAT CGCCTTGGTC AGGAGGTGTG CGGCTCTGGT 
CATCACAAAA AAGGTGACCG CAGAGCTGAT GGAGACCGCT GTGCTTTGGT GACAGACTCT 
GCTCCTGAGA GGGAGCTTTC TCCCTTACTC TCCTTGCCTT CCCCAGTGCC CCCCCTGTCA 
TCTGTACATT CCAACCAGCA AGCTCTTCCC CGGACACAAG GAAGCAGCAA GATTCACAGC 
AGTAACAGTA ATAGTAAAGG CTATTGCCCA GCCAAATCTC CCAAAGACCT AGTAGCAAAG 
GTCCATGATA AAGAGACCCC TCAAGACAAT TTAGTGGCAG TTGCCGGCCT TGGGGTGGCC 
CCTCCCCAGC CACCTTCTCA GACTTTCCCC CCACCTTCCC TCCCCTCAAA AACTGTTGCG 
ATGCAGCAGA AGCCCACGGC TTACGTCCGG CCCATGGACG GTCAAGATCA GGCTCCTAGT 
GAGTCACCTG AACTGAAACC ACTGCCGGAG GACTACCGGC AACAGACCTT TGAAAAAACA 
GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACCAAACTGA AGATGCCATC TCAGTCGGGT 
GAGCAGACCT ACTCCAATGA AGTTCATTGT GTTGAAGAGA TTTTGAAGGA AATGACCCAT 
TCATGGCCAC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CTGCTGAGCC CTCCAAATTT 
CCTTTTCCCA CAAAGGATTC TCAGCACATT AGTTCTGTGA CCCAAAATCA AAAACAATAT 
GATACATCTT CAAAAACCCA CACTAATTCT CAGCAGGGAA CATCCATGCT TGAAGATGAC 
CTTCAGCTCA GTGACAGTGA GGACAGTGAC AACGAACAAA GCCCAGAGAA GCCTCCTCCT 
GCGTCTGCGC CTCCCAGTGC CCCGCAGTCG CTTCCAGAAG CAGCGGCCTC AGCACATTCC 
AGTAGTGCCG AGTCAGAGAG CACCAGTGAC TCAGACAGCT CCTCGGACTC CGAGAGCGAG 
AGCAGCTCAA GTGACAGCGA AGAGAATGAG CCCCTAGCAG CTCCGACTCC TGAGCCTGAG 
CCTCCTACAA CAAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAACCGGCA 
GCCCCACCAG ACACCCAGGA GGGGAGCGCA GAGCCCCCGC CTCGGCACCC AGACAGTAAG 
GGCAAGGGGG GAGGCGACAG CAGCGGCCCC ACCACTGGTC ACGAGCTCTC GGAATCCAAA 
GATCCTCCCC TCAAGAGCTC CGGCAAAGCC CCCCGGGGCT CCTCCGAGGG CCCTCACCCC 
GGCAGGAGGA GCTGTCCGAA GTCCCCGGCC CAACAGGAGG CCCTGCCGAG GCAGACTGTG 
GGAACCAAAC AACCCAAGAA GCCGGGCAAG GCCTCCACGC CGGCGGACCC TCGGGCCGGC 
CTGCAGGTGG AGAGCGAGCC GGGCCCCCCT CCGTCCGGCT CCAGAGACCA GCCTTCCAAA 
GACAAGCCCA AGGTGAAGAC GAAAGGGAGG CCGCGCGCCG GCGACAGCAG GGAGCCCAAG 
CCCGCCGCGC CCAGCCCCGG TGACCGCAAG AAGCACCAGA GCGGGCCGCT GGCCCCCTCA 
AAGGCACTCT CGGGCCCCGA GCCCACGAAG GACAATGCGG GGGCCAGGAG CCCCGAGCAC 
TTTGCCCTGG TTCCCCTGAC CCAGAGCCAG GGCCCGCCCC ACGGCGGTGG CGGCAGGACT 
AGTGGCTGCC GGCGCGCAGT GGTGGTCCAG GAGGACCGCA GGAAGGACAA GCTCCCGGGG 
CCTGCCAGAG ACACCAAGCT GCTGTCGCCG CTCAGGGACC CTCCTCCCCC ACCCATCTTG 
ATGGTGAAGA TAACCCTGGA CCTTCTTTCT CGGATACCCC AGCTTCCTGG GAAAGGGGGC 
CGGCAGAAGA AGCCAGAGGA CAAGCAGCCC CCCACAGGGA AGAAGCCCGA TTTGGAGAAG 
AGAGGCGCAG ACAACTCCAG CAAGTTGGTC AAAAAAAGAA AGGCTGAAGC TGAGAAAGAC 
CATGACAGCA AGAAAGCCAG ACTGGAGAAA GAAACCAAGT TGCAGTCATT ATCTTCATCC 
CCCCACAAAG AATCTTCCAA AACAAAAACA CCCAGGCCCT CCTCTGAGCC CTCAAAGAAG 
GAGATGCTTC CCCCCCCACC TGTGTCATCC TCATCCTCAT CCTCCCAGAA GCCAGCCAAG 
CCTGCACAAA AGAGGCCAAG GCGGGAAGCT GATCCCTCCA GCCAAGACGC TTCCCAAAGT 
GCCAGTAGTA GCAAGGGCAA CCACAAAGAC TCTTCCACTC CCAAGCACAG AAAAGTAGAG 
GGGAAGGGAT CTGGAAGCAG TACAGAGCAC AAGGGATCTT CTGGAGATAG TGCATATCCT 
TTTCTAGTGC CTTCTTTGCC AAATGGTAAC TCTAAACCAG GGAAGCCTCC AGTGAAGCTT 
GACAGACAAC AAGCAGATTT TCACAGGAAG GAGGCCAAAA AGTTGAAGCA AAAAGCAGAG 
TTAATGATGG ACAAGGTTGG GAAGGCTTTT AAGTACTTGG AGGCCGCCTT GTCCTTAATT 
GAGTGTGGGA TTGCCATGGA GTCGGAAAGC CCAGCCTCCA AATCGGCTTA CTCGGTGTAC 
TCCGAAACCG TAGACCTCAT TAAATTTATA ATGTCATTAA AATCCTTCTC AGACGCCTCA 
TCTCCACAAG AGAAAATATT TGCTGTTTTA TGCATGCGTT GTCAGTCCAT TTTGAACATG 
GCGATGTTTC GTTGTAAAAA AGATATAGCA ATAAAGTATT CTCGTGCTCT TAACGAACAC 
TTCAGCAGTT TTTTCAAAGT TGCCCAGGCA CCTTCTCCAT GCATTGCAAG AAACACAGGC 
ACACCGTCCC CTCTCTCTCC AGTTCCTTCT CCTGCCAGCT CCGTAGGGTC CCAGTCCAGT 
GCTGGCAGCA TAGGGAGCAG TGGTATGCCT GTCACCATCC AAAACATGAC ATCTTCTTAT 
GTCACCATTA CTTCCCATGT CCTTACCGCC TTTGACCTCT GGGAACAGGC TGAGGTCCTT 
ACAAGGAAGA ATAAAGAATT TTTTGCTCAG CTCAGCACAA GTGTGTGTGC CTTGGCCCTC 
AACAGCAGTT TGATGGATCT GGTGCACTAT ACAAGACAGG GTTTTCAGCG GCTAAAACAA 
GCAACCAAAA CACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965433.1
CDS	316..3993
Translation	MAFTERVQSSSSSSLYNEDRNLLRIREKERRNQEAHQEKEAFSEKIPLFGEPYKTEKGDELSSRIQNMLGNYEEVKEFFSSKSHPHRLDASENRLGKPRYPLFPEKGSSIPSHSFHTSAHHQPVNTPASGPLPVGNVSQNPKMAQPRMEPMSNPHVKSYGPPDSQHPTQDRLGQEVCGSGHHKKGDRRADGDRCALVTDSAPERELSPLLSLPSPVPPLSSVHSNQQALPRTQGSSKIHSSNSNSKGYCPAKSPKDLVAKVHDKETPQDNLVAVAGLGVAPPQPPSQTFPPPSLPSKTVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSGEQTYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHISSVTQNQKQYDTSSKTHTNSQQGTSMLEDDLQLSDSEDSDNEQSPEKPPPASAPPSAPQSLPEAAASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLAAPTPEPEPPTTNKWQLDNWLTKVSQPAAPPDTQEGSAEPPPRHPDSKGKGGGDSSGPTTGHELSESKDPPLKSSGKAPRGSSEGPHPGRRSCPKSPAQQEALPRQTVGTKQPKKPGKASTPADPRAGLQVESEPGPPPSGSRDQPSKDKPKVKTKGRPRAGDSREPKPAAPSPGDRKKHQSGPLAPSKALSGPEPTKDNAGARSPEHFALVPLTQSQGPPHGGGGRTSGCRRAVVVQEDRRKDKLPGPARDTKLLSPLRDPPPPPILMVKITLDLLSRIPQLPGKGGRQKKPEDKQPPTGKKPDLEKRGADNSSKLVKKRKAEAEKDHDSKKARLEKETKLQSLSSSPHKESSKTKTPRPSSEPSKKEMLPPPPVSSSSSSSQKPAKPAQKRPRREADPSSQDASQSASSSKGNHKDSSTPKHRKVEGKGSGSSTEHKGSSGDSAYPFLVPSLPNGNSKPGKPPVKLDRQQADFHRKEAKKLKQKAELMMDKVGKAFKYLEAALSLIECGIAMESESPASKSAYSVYSETVDLIKFIMSLKSFSDASSPQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRALNEHFSSFFKVAQAPSPCIARNTGTPSPLSPVPSPASSVGSQSSAGSIGSSGMPVTIQNMTSSYVTITSHVLTAFDLWEQAEVLTRKNKEFFAQLSTSVCALALNSSLMDLVHYTRQGFQRLKQATKTP

Target ORF information:

RefSeq Version	XM_014109958.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris AF4/FMR2 family, member 1 (AFF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109958.1

ORF Insert Sequence:

ATGGCGTTTA CTGAAAGAGT CCAGAGCAGC AGTAGCAGCA GCTTGTACAA TGAAGACCGG 
AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGAGAAAGAG 
GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCCTACA AGACAGAAAA AGGTGATGAG 
CTATCCAGTC GAATACAGAA CATGTTGGGA AACTATGAAG AAGTGAAGGA GTTCTTTAGC 
AGCAAGTCCC ACCCTCATCG CTTGGATGCC TCTGAAAATA GGTTGGGAAA GCCAAGATAT 
CCTTTATTTC CTGAAAAGGG GAGCAGCATT CCATCCCACT CCTTCCACAC GAGTGCCCAC 
CACCAGCCTG TTAATACTCC TGCCTCCGGA CCACTTCCTG TTGGTAACGT AAGCCAGAAC 
CCAAAGATGG CACAGCCAAG AATGGAACCA ATGTCAAATC CTCATGTCAA AAGCTATGGC 
CCACCAGACA GCCAGCACCC AACCCAAGAT CGCCTTGGTC AGGAGGTGTG CGGCTCTGGT 
CATCACAAAA AAGGTGACCG CAGAGCTGAT GGAGACCGCT GTGCTTTGGT GACAGACTCT 
GCTCCTGAGA GGGAGCTTTC TCCCTTACTC TCCTTGCCTT CCCCAGTGCC CCCCCTGTCA 
TCTGTACATT CCAACCAGCA AGCTCTTCCC CGGACACAAG GAAGCAGCAA GATTCACAGC 
AGTAACAGTA ATAGTAAAGG CTATTGCCCA GCCAAATCTC CCAAAGACCT AGTAGCAAAG 
GTCCATGATA AAGAGACCCC TCAAGACAAT TTAGTGGCAG TTGCCGGCCT TGGGGTGGCC 
CCTCCCCAGC CACCTTCTCA GACTTTCCCC CCACCTTCCC TCCCCTCAAA AACTGTTGCG 
ATGCAGCAGA AGCCCACGGC TTACGTCCGG CCCATGGACG GTCAAGATCA GGCTCCTAGT 
GAGTCACCTG AACTGAAACC ACTGCCGGAG GACTACCGGC AACAGACCTT TGAAAAAACA 
GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACCAAACTGA AGATGCCATC TCAGTCGGGT 
GAGCAGACCT ACTCCAATGA AGTTCATTGT GTTGAAGAGA TTTTGAAGGA AATGACCCAT 
TCATGGCCAC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CTGCTGAGCC CTCCAAATTT 
CCTTTTCCCA CAAAGGATTC TCAGCACATT AGTTCTGTGA CCCAAAATCA AAAACAATAT 
GATACATCTT CAAAAACCCA CACTAATTCT CAGCAGGGAA CATCCATGCT TGAAGATGAC 
CTTCAGCTCA GTGACAGTGA GGACAGTGAC AACGAACAAA GCCCAGAGAA GCCTCCTCCT 
GCGTCTGCGC CTCCCAGTGC CCCGCAGTCG CTTCCAGAAG CAGCGGCCTC AGCACATTCC 
AGTAGTGCCG AGTCAGAGAG CACCAGTGAC TCAGACAGCT CCTCGGACTC CGAGAGCGAG 
AGCAGCTCAA GTGACAGCGA AGAGAATGAG CCCCTAGCAG CTCCGACTCC TGAGCCTGAG 
CCTCCTACAA CAAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAACCGGCA 
GCCCCACCAG ACACCCAGGA GGGGAGCGCA GAGCCCCCGC CTCGGCACCC AGACAGTAAG 
GGCAAGGGGG GAGGCGACAG CAGCGGCCCC ACCACTGGTC ACGAGCTCTC GGAATCCAAA 
GATCCTCCCC TCAAGAGCTC CGGCAAAGCC CCCCGGGGCT CCTCCGAGGG CCCTCACCCC 
GGCAGGAGGA GCTGTCCGAA GTCCCCGGCC CAACAGGAGG CCCTGCCGAG GCAGACTGTG 
GGAACCAAAC AACCCAAGAA GCCGGGCAAG GCCTCCACGC CGGCGGACCC TCGGGCCGGC 
CTGCAGGTGG AGAGCGAGCC GGGCCCCCCT CCGTCCGGCT CCAGAGACCA GCCTTCCAAA 
GACAAGCCCA AGGTGAAGAC GAAAGGGAGG CCGCGCGCCG GCGACAGCAG GGAGCCCAAG 
CCCGCCGCGC CCAGCCCCGG TGACCGCAAG AAGCACCAGA GCGGGCCGCT GGCCCCCTCA 
AAGGCACTCT CGGGCCCCGA GCCCACGAAG GACAATGCGG GGGCCAGGAG CCCCGAGCAC 
TTTGCCCTGG TTCCCCTGAC CCAGAGCCAG GGCCCGCCCC ACGGCGGTGG CGGCAGGACT 
AGTGGCTGCC GGCGCGCAGT GGTGGTCCAG GAGGACCGCA GGAAGGACAA GCTCCCGGGG 
CCTGCCAGAG ACACCAAGCT GCTGTCGCCG CTCAGGGACC CTCCTCCCCC ACCCATCTTG 
ATGGTGAAGA TAACCCTGGA CCTTCTTTCT CGGATACCCC AGCTTCCTGG GAAAGGGGGC 
CGGCAGAAGA AGCCAGAGGA CAAGCAGCCC CCCACAGGGA AGAAGCCCGA TTTGGAGAAG 
AGAGGCGCAG ACAACTCCAG CAAGTTGGTC AAAAAAAGAA AGGCTGAAGC TGAGAAAGAC 
CATGACAGCA AGAAAGCCAG ACTGGAGAAA GAAACCAAGT TGCAGTCATT ATCTTCATCC 
CCCCACAAAG AATCTTCCAA AACAAAAACA CCCAGGCCCT CCTCTGAGCC CTCAAAGAAG 
GAGATGCTTC CCCCCCCACC TGTGTCATCC TCATCCTCAT CCTCCCAGAA GCCAGCCAAG 
CCTGCACAAA AGAGGCCAAG GCGGGAAGCT GATCCCTCCA GCCAAGACGC TTCCCAAAGT 
GCCAGTAGTA GCAAGGGCAA CCACAAAGAC TCTTCCACTC CCAAGCACAG AAAAGTAGAG 
GGGAAGGGAT CTGGAAGCAG TACAGAGCAC AAGGGATCTT CTGGAGATAG TGCATATCCT 
TTTCTAGTGC CTTCTTTGCC AAATGGTAAC TCTAAACCAG GGAAGCCTCC AGTGAAGCTT 
GACAGACAAC AAGCAGATTT TCACAGGAAG GAGGCCAAAA AGTTGAAGCA AAAAGCAGAG 
TTAATGATGG ACAAGGTTGG GAAGGCTTTT AAGTACTTGG AGGCCGCCTT GTCCTTAATT 
GAGTGTGGGA TTGCCATGGA GTCGGAAAGC CCAGCCTCCA AATCGGCTTA CTCGGTGTAC 
TCCGAAACCG TAGACCTCAT TAAATTTATA ATGTCATTAA AATCCTTCTC AGACGCCTCA 
TCTCCACAAG AGAAAATATT TGCTGTTTTA TGCATGCGTT GTCAGTCCAT TTTGAACATG 
GCGATGTTTC GTTGTAAAAA AGATATAGCA ATAAAGTATT CTCGTGCTCT TAACGAACAC 
TTCAGCAGTT TTTTCAAAGT TGCCCAGGCA CCTTCTCCAT GCATTGCAAG AAACACAGGC 
ACACCGTCCC CTCTCTCTCC AGTTCCTTCT CCTGCCAGCT CCGTAGGGTC CCAGTCCAGT 
GCTGGCAGCA TAGGGAGCAG TGGTATGCCT GTCACCATCC AAAACATGAC ATCTTCTTAT 
GTCACCATTA CTTCCCATGT CCTTACCGCC TTTGACCTCT GGGAACAGGC TGAGGTCCTT 
ACAAGGAAGA ATAAAGAATT TTTTGCTCAG CTCAGCACAA GTGTGTGTGC CTTGGCCCTC 
AACAGCAGTT TGATGGATCT GGTGCACTAT ACAAGACAGG GTTTTCAGCG GCTAAAACAA 
GCAACCAAAA CACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_005639159.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005639216.1 Canis lupus familiaris AF4/FMR2 family, member 1 (AFF1), transcript variant X3, mRNA.

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CloneID	OCe29734
Clone ID Related Accession (Same CDS sequence)	XM_005639159.2
Accession Version	XM_005639159.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	AF4/FMR2 family member 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005639159.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCCC AGTCCAGCTT GTACAATGAA GACCGGAACC TGCTTCGAAT TAGAGAGAAG 
GAAAGACGCA ACCAGGAAGC TCACCAAGAG AAAGAGGCAT TTTCTGAAAA GATCCCCCTT 
TTTGGAGAGC CCTACAAGAC AGAAAAAGGT GATGAGCTAT CCAGTCGAAT ACAGAACATG 
TTGGGAAACT ATGAAGAAGT GAAGGAGTTC TTTAGCAGCA AGTCCCACCC TCATCGCTTG 
GATGCCTCTG AAAATAGGTT GGGAAAGCCA AGATATCCTT TATTTCCTGA AAAGGGGAGC 
AGCATTCCAT CCCACTCCTT CCACACGAGT GCCCACCACC AGCCTGTTAA TACTCCTGCC 
TCCGGACCAC TTCCTGTTGG TAACGTAAGC CAGAACCCAA AGATGGCACA GCCAAGAATG 
GAACCAATGT CAAATCCTCA TGTCAAAAGC TATGGCCCAC CAGACAGCCA GCACCCAACC 
CAAGATCGCC TTGGTCAGGA GGTGTGCGGC TCTGGTCATC ACAAAAAAGG TGACCGCAGA 
GCTGATGGAG ACCGCTGTGC TTTGGTGACA GACTCTGCTC CTGAGAGGGA GCTTTCTCCC 
TTACTCTCCT TGCCTTCCCC AGTGCCCCCC CTGTCATCTG TACATTCCAA CCAGCAAGCT 
CTTCCCCGGA CACAAGGAAG CAGCAAGATT CACAGCAGTA ACAGTAATAG TAAAGGCTAT 
TGCCCAGCCA AATCTCCCAA AGACCTAGTA GCAAAGGTCC ATGATAAAGA GACCCCTCAA 
GACAATTTAG TGGCAGTTGC CGGCCTTGGG GTGGCCCCTC CCCAGCCACC TTCTCAGACT 
TTCCCCCCAC CTTCCCTCCC CTCAAAAACT GTTGCGATGC AGCAGAAGCC CACGGCTTAC 
GTCCGGCCCA TGGACGGTCA AGATCAGGCT CCTAGTGAGT CACCTGAACT GAAACCACTG 
CCGGAGGACT ACCGGCAACA GACCTTTGAA AAAACAGACT TAAAAGTGCC TGCCAAAGCC 
AAGCTCACCA AACTGAAGAT GCCATCTCAG TCGGGTGAGC AGACCTACTC CAATGAAGTT 
CATTGTGTTG AAGAGATTTT GAAGGAAATG ACCCATTCAT GGCCACCTCC TTTGACAGCA 
ATACACACGC CTAGTACTGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG 
CACATTAGTT CTGTGACCCA AAATCAAAAA CAATATGATA CATCTTCAAA AACCCACACT 
AATTCTCAGC AGGGAACATC CATGCTTGAA GATGACCTTC AGCTCAGTGA CAGTGAGGAC 
AGTGACAACG AACAAAGCCC AGAGAAGCCT CCTCCTGCGT CTGCGCCTCC CAGTGCCCCG 
CAGTCGCTTC CAGAAGCAGC GGCCTCAGCA CATTCCAGTA GTGCCGAGTC AGAGAGCACC 
AGTGACTCAG ACAGCTCCTC GGACTCCGAG AGCGAGAGCA GCTCAAGTGA CAGCGAAGAG 
AATGAGCCCC TAGCAGCTCC GACTCCTGAG CCTGAGCCTC CTACAACAAA CAAATGGCAG 
CTGGACAACT GGCTGACCAA AGTCAGCCAA CCGGCAGCCC CACCAGACAC CCAGGAGGGG 
AGCGCAGAGC CCCCGCCTCG GCACCCAGAC AGTAAGGGCA AGGGGGGAGG CGACAGCAGC 
GGCCCCACCA CTGGTCACGA GCTCTCGGAA TCCAAAGATC CTCCCCTCAA GAGCTCCGGC 
AAAGCCCCCC GGGGCTCCTC CGAGGGCCCT CACCCCGGCA GGAGGAGCTG TCCGAAGTCC 
CCGGCCCAAC AGGAGGCCCT GCCGAGGCAG ACTGTGGGAA CCAAACAACC CAAGAAGCCG 
GGCAAGGCCT CCACGCCGGC GGACCCTCGG GCCGGCCTGC AGGTGGAGAG CGAGCCGGGC 
CCCCCTCCGT CCGGCTCCAG AGACCAGCCT TCCAAAGACA AGCCCAAGGT GAAGACGAAA 
GGGAGGCCGC GCGCCGGCGA CAGCAGGGAG CCCAAGCCCG CCGCGCCCAG CCCCGGTGAC 
CGCAAGAAGC ACCAGAGCGG GCCGCTGGCC CCCTCAAAGG CACTCTCGGG CCCCGAGCCC 
ACGAAGGACA ATGCGGGGGC CAGGAGCCCC GAGCACTTTG CCCTGGTTCC CCTGACCCAG 
AGCCAGGGCC CGCCCCACGG CGGTGGCGGC AGGACTAGTG GCTGCCGGCG CGCAGTGGTG 
GTCCAGGAGG ACCGCAGGAA GGACAAGCTC CCGGGGCCTG CCAGAGACAC CAAGCTGCTG 
TCGCCGCTCA GGGACCCTCC TCCCCCACCC ATCTTGATGG TGAAGATAAC CCTGGACCTT 
CTTTCTCGGA TACCCCAGCT TCCTGGGAAA GGGGGCCGGC AGAAGAAGCC AGAGGACAAG 
CAGCCCCCCA CAGGGAAGAA GCCCGATTTG GAGAAGAGAG GCGCAGACAA CTCCAGCAAG 
TTGGTCAAAA AAAGAAAGGC TGAAGCTGAG AAAGACCATG ACAGCAAGAA AGCCAGACTG 
GAGAAAGAAA CCAAGTTGCA GTCATTATCT TCATCCCCCC ACAAAGAATC TTCCAAAACA 
AAAACACCCA GGCCCTCCTC TGAGCCCTCA AAGAAGGAGA TGCTTCCCCC CCCACCTGTG 
TCATCCTCAT CCTCATCCTC CCAGAAGCCA GCCAAGCCTG CACAAAAGAG GCCAAGGCGG 
GAAGCTGATC CCTCCAGCCA AGACGCTTCC CAAAGTGCCA GTAGTAGCAA GGGCAACCAC 
AAAGACTCTT CCACTCCCAA GCACAGAAAA GTAGAGGGGA AGGGATCTGG AAGCAGTACA 
GAGCACAAGG GATCTTCTGG AGATAGTGCA TATCCTTTTC TAGTGCCTTC TTTGCCAAAT 
GGTAACTCTA AACCAGGGAA GCCTCCAGTG AAGCTTGACA GACAACAAGC AGATTTTCAC 
AGGAAGGAGG CCAAAAAGTT GAAGCAAAAA GCAGAGTTAA TGATGGACAA GGTTGGGAAG 
GCTTTTAAGT ACTTGGAGGC CGCCTTGTCC TTAATTGAGT GTGGGATTGC CATGGAGTCG 
GAAAGCCCAG CCTCCAAATC GGCTTACTCG GTGTACTCCG AAACCGTAGA CCTCATTAAA 
TTTATAATGT CATTAAAATC CTTCTCAGAC GCCTCATCTC CACAAGAGAA AATATTTGCT 
GTTTTATGCA TGCGTTGTCA GTCCATTTTG AACATGGCGA TGTTTCGTTG TAAAAAAGAT 
ATAGCAATAA AGTATTCTCG TGCTCTTAAC GAACACTTCA GCAGTTTTTT CAAAGTTGCC 
CAGGCACCTT CTCCATGCAT TGCAAGAAAC ACAGGCACAC CGTCCCCTCT CTCTCCAGTT 
CCTTCTCCTG CCAGCTCCGT AGGGTCCCAG TCCAGTGCTG GCAGCATAGG GAGCAGTGGT 
ATGCCTGTCA CCATCCAAAA CATGACATCT TCTTATGTCA CCATTACTTC CCATGTCCTT 
ACCGCCTTTG ACCTCTGGGA ACAGGCTGAG GTCCTTACAA GGAAGAATAA AGAATTTTTT 
GCTCAGCTCA GCACAAGTGT GTGTGCCTTG GCCCTCAACA GCAGTTTGAT GGATCTGGTG 
CACTATACAA GACAGGGTTT TCAGCGGCTA AAACAAGCAA CCAAAACACC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005639216.1
CDS	67..3720
Translation	MAAQSSLYNEDRNLLRIREKERRNQEAHQEKEAFSEKIPLFGEPYKTEKGDELSSRIQNMLGNYEEVKEFFSSKSHPHRLDASENRLGKPRYPLFPEKGSSIPSHSFHTSAHHQPVNTPASGPLPVGNVSQNPKMAQPRMEPMSNPHVKSYGPPDSQHPTQDRLGQEVCGSGHHKKGDRRADGDRCALVTDSAPERELSPLLSLPSPVPPLSSVHSNQQALPRTQGSSKIHSSNSNSKGYCPAKSPKDLVAKVHDKETPQDNLVAVAGLGVAPPQPPSQTFPPPSLPSKTVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSGEQTYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHISSVTQNQKQYDTSSKTHTNSQQGTSMLEDDLQLSDSEDSDNEQSPEKPPPASAPPSAPQSLPEAAASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLAAPTPEPEPPTTNKWQLDNWLTKVSQPAAPPDTQEGSAEPPPRHPDSKGKGGGDSSGPTTGHELSESKDPPLKSSGKAPRGSSEGPHPGRRSCPKSPAQQEALPRQTVGTKQPKKPGKASTPADPRAGLQVESEPGPPPSGSRDQPSKDKPKVKTKGRPRAGDSREPKPAAPSPGDRKKHQSGPLAPSKALSGPEPTKDNAGARSPEHFALVPLTQSQGPPHGGGGRTSGCRRAVVVQEDRRKDKLPGPARDTKLLSPLRDPPPPPILMVKITLDLLSRIPQLPGKGGRQKKPEDKQPPTGKKPDLEKRGADNSSKLVKKRKAEAEKDHDSKKARLEKETKLQSLSSSPHKESSKTKTPRPSSEPSKKEMLPPPPVSSSSSSSQKPAKPAQKRPRREADPSSQDASQSASSSKGNHKDSSTPKHRKVEGKGSGSSTEHKGSSGDSAYPFLVPSLPNGNSKPGKPPVKLDRQQADFHRKEAKKLKQKAELMMDKVGKAFKYLEAALSLIECGIAMESESPASKSAYSVYSETVDLIKFIMSLKSFSDASSPQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRALNEHFSSFFKVAQAPSPCIARNTGTPSPLSPVPSPASSVGSQSSAGSIGSSGMPVTIQNMTSSYVTITSHVLTAFDLWEQAEVLTRKNKEFFAQLSTSVCALALNSSLMDLVHYTRQGFQRLKQATKTP

Target ORF information:

RefSeq Version	XM_005639159.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris AF4/FMR2 family, member 1 (AFF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005639159.2

ORF Insert Sequence:

ATGGCAGCCC AGTCCAGCTT GTACAATGAA GACCGGAACC TGCTTCGAAT TAGAGAGAAG 
GAAAGACGCA ACCAGGAAGC TCACCAAGAG AAAGAGGCAT TTTCTGAAAA GATCCCCCTT 
TTTGGAGAGC CCTACAAGAC AGAAAAAGGT GATGAGCTAT CCAGTCGAAT ACAGAACATG 
TTGGGAAACT ATGAAGAAGT GAAGGAGTTC TTTAGCAGCA AGTCCCACCC TCATCGCTTG 
GATGCCTCTG AAAATAGGTT GGGAAAGCCA AGATATCCTT TATTTCCTGA AAAGGGGAGC 
AGCATTCCAT CCCACTCCTT CCACACGAGT GCCCACCACC AGCCTGTTAA TACTCCTGCC 
TCCGGACCAC TTCCTGTTGG TAACGTAAGC CAGAACCCAA AGATGGCACA GCCAAGAATG 
GAACCAATGT CAAATCCTCA TGTCAAAAGC TATGGCCCAC CAGACAGCCA GCACCCAACC 
CAAGATCGCC TTGGTCAGGA GGTGTGCGGC TCTGGTCATC ACAAAAAAGG TGACCGCAGA 
GCTGATGGAG ACCGCTGTGC TTTGGTGACA GACTCTGCTC CTGAGAGGGA GCTTTCTCCC 
TTACTCTCCT TGCCTTCCCC AGTGCCCCCC CTGTCATCTG TACATTCCAA CCAGCAAGCT 
CTTCCCCGGA CACAAGGAAG CAGCAAGATT CACAGCAGTA ACAGTAATAG TAAAGGCTAT 
TGCCCAGCCA AATCTCCCAA AGACCTAGTA GCAAAGGTCC ATGATAAAGA GACCCCTCAA 
GACAATTTAG TGGCAGTTGC CGGCCTTGGG GTGGCCCCTC CCCAGCCACC TTCTCAGACT 
TTCCCCCCAC CTTCCCTCCC CTCAAAAACT GTTGCGATGC AGCAGAAGCC CACGGCTTAC 
GTCCGGCCCA TGGACGGTCA AGATCAGGCT CCTAGTGAGT CACCTGAACT GAAACCACTG 
CCGGAGGACT ACCGGCAACA GACCTTTGAA AAAACAGACT TAAAAGTGCC TGCCAAAGCC 
AAGCTCACCA AACTGAAGAT GCCATCTCAG TCGGGTGAGC AGACCTACTC CAATGAAGTT 
CATTGTGTTG AAGAGATTTT GAAGGAAATG ACCCATTCAT GGCCACCTCC TTTGACAGCA 
ATACACACGC CTAGTACTGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG 
CACATTAGTT CTGTGACCCA AAATCAAAAA CAATATGATA CATCTTCAAA AACCCACACT 
AATTCTCAGC AGGGAACATC CATGCTTGAA GATGACCTTC AGCTCAGTGA CAGTGAGGAC 
AGTGACAACG AACAAAGCCC AGAGAAGCCT CCTCCTGCGT CTGCGCCTCC CAGTGCCCCG 
CAGTCGCTTC CAGAAGCAGC GGCCTCAGCA CATTCCAGTA GTGCCGAGTC AGAGAGCACC 
AGTGACTCAG ACAGCTCCTC GGACTCCGAG AGCGAGAGCA GCTCAAGTGA CAGCGAAGAG 
AATGAGCCCC TAGCAGCTCC GACTCCTGAG CCTGAGCCTC CTACAACAAA CAAATGGCAG 
CTGGACAACT GGCTGACCAA AGTCAGCCAA CCGGCAGCCC CACCAGACAC CCAGGAGGGG 
AGCGCAGAGC CCCCGCCTCG GCACCCAGAC AGTAAGGGCA AGGGGGGAGG CGACAGCAGC 
GGCCCCACCA CTGGTCACGA GCTCTCGGAA TCCAAAGATC CTCCCCTCAA GAGCTCCGGC 
AAAGCCCCCC GGGGCTCCTC CGAGGGCCCT CACCCCGGCA GGAGGAGCTG TCCGAAGTCC 
CCGGCCCAAC AGGAGGCCCT GCCGAGGCAG ACTGTGGGAA CCAAACAACC CAAGAAGCCG 
GGCAAGGCCT CCACGCCGGC GGACCCTCGG GCCGGCCTGC AGGTGGAGAG CGAGCCGGGC 
CCCCCTCCGT CCGGCTCCAG AGACCAGCCT TCCAAAGACA AGCCCAAGGT GAAGACGAAA 
GGGAGGCCGC GCGCCGGCGA CAGCAGGGAG CCCAAGCCCG CCGCGCCCAG CCCCGGTGAC 
CGCAAGAAGC ACCAGAGCGG GCCGCTGGCC CCCTCAAAGG CACTCTCGGG CCCCGAGCCC 
ACGAAGGACA ATGCGGGGGC CAGGAGCCCC GAGCACTTTG CCCTGGTTCC CCTGACCCAG 
AGCCAGGGCC CGCCCCACGG CGGTGGCGGC AGGACTAGTG GCTGCCGGCG CGCAGTGGTG 
GTCCAGGAGG ACCGCAGGAA GGACAAGCTC CCGGGGCCTG CCAGAGACAC CAAGCTGCTG 
TCGCCGCTCA GGGACCCTCC TCCCCCACCC ATCTTGATGG TGAAGATAAC CCTGGACCTT 
CTTTCTCGGA TACCCCAGCT TCCTGGGAAA GGGGGCCGGC AGAAGAAGCC AGAGGACAAG 
CAGCCCCCCA CAGGGAAGAA GCCCGATTTG GAGAAGAGAG GCGCAGACAA CTCCAGCAAG 
TTGGTCAAAA AAAGAAAGGC TGAAGCTGAG AAAGACCATG ACAGCAAGAA AGCCAGACTG 
GAGAAAGAAA CCAAGTTGCA GTCATTATCT TCATCCCCCC ACAAAGAATC TTCCAAAACA 
AAAACACCCA GGCCCTCCTC TGAGCCCTCA AAGAAGGAGA TGCTTCCCCC CCCACCTGTG 
TCATCCTCAT CCTCATCCTC CCAGAAGCCA GCCAAGCCTG CACAAAAGAG GCCAAGGCGG 
GAAGCTGATC CCTCCAGCCA AGACGCTTCC CAAAGTGCCA GTAGTAGCAA GGGCAACCAC 
AAAGACTCTT CCACTCCCAA GCACAGAAAA GTAGAGGGGA AGGGATCTGG AAGCAGTACA 
GAGCACAAGG GATCTTCTGG AGATAGTGCA TATCCTTTTC TAGTGCCTTC TTTGCCAAAT 
GGTAACTCTA AACCAGGGAA GCCTCCAGTG AAGCTTGACA GACAACAAGC AGATTTTCAC 
AGGAAGGAGG CCAAAAAGTT GAAGCAAAAA GCAGAGTTAA TGATGGACAA GGTTGGGAAG 
GCTTTTAAGT ACTTGGAGGC CGCCTTGTCC TTAATTGAGT GTGGGATTGC CATGGAGTCG 
GAAAGCCCAG CCTCCAAATC GGCTTACTCG GTGTACTCCG AAACCGTAGA CCTCATTAAA 
TTTATAATGT CATTAAAATC CTTCTCAGAC GCCTCATCTC CACAAGAGAA AATATTTGCT 
GTTTTATGCA TGCGTTGTCA GTCCATTTTG AACATGGCGA TGTTTCGTTG TAAAAAAGAT 
ATAGCAATAA AGTATTCTCG TGCTCTTAAC GAACACTTCA GCAGTTTTTT CAAAGTTGCC 
CAGGCACCTT CTCCATGCAT TGCAAGAAAC ACAGGCACAC CGTCCCCTCT CTCTCCAGTT 
CCTTCTCCTG CCAGCTCCGT AGGGTCCCAG TCCAGTGCTG GCAGCATAGG GAGCAGTGGT 
ATGCCTGTCA CCATCCAAAA CATGACATCT TCTTATGTCA CCATTACTTC CCATGTCCTT 
ACCGCCTTTG ACCTCTGGGA ACAGGCTGAG GTCCTTACAA GGAAGAATAA AGAATTTTTT 
GCTCAGCTCA GCACAAGTGT GTGTGCCTTG GCCCTCAACA GCAGTTTGAT GGATCTGGTG 
CACTATACAA GACAGGGTTT TCAGCGGCTA AAACAAGCAA CCAAAACACC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

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japanmarket@genscript.com

電話

03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

AFF1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報