AFF1 cDNA ORF clone, Homo sapiens(human)

The following AFF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu59003 NM_001313960.1
Latest version!
Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu59003 XM_017008215.2
Latest version!
Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu59003 XM_017008216.1
Latest version!
Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu24271 NM_005935.3
Latest version!
Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu25298 NM_001166693.2
Latest version!
Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu38943 NM_001313959.1
Latest version!
Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu25298 XM_005263007.3
Latest version!
Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu25298 XM_011531973.3
Latest version!
Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu38940 XM_005263009.4
Latest version!
Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu38942 XM_005263011.3
Latest version!
Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu38944 XM_005263013.4
Latest version!
Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu38940 XM_005263009.3 Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu59003 XM_017008215.1 Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu25298 XM_011531973.2 Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu38944 XM_005263013.3 Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu59003
    Clone ID Related Accession (Same CDS sequence) XM_017008216.1 , XM_017008215.2 , NM_001313960.1 , XM_017008215.1
    Accession Version NM_001313960.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-04
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L13773.1, AK296243.1, BC114930.1, AC092658.2 and AI638134.1. On Sep 16, 2015 this sequence version replaced XM_011531974.1. Transcript Variant: This variant (4) contains a distinct 5' UTR, uses an alternate translation start site, and lacks two alternate, in-frame exons compared to variant 1. It encodes isoform 4, which is shorter than and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296243.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGACCCATT CATGGCCGCC TCCTTTGACA GCAATACATA CGCCTAGTAC AGCTGAGCCA 
    TCCAAGTTTC CTTTCCCTAC AAAGGACTCT CAGCATGTCA GTTCTGTAAC CCAAAACCAA
    AAACAATATG ATACATCTTC AAAAACTCAC TCAAATTCTC AGCAAGGAAC GTCATCCATG
    CTCGAAGACG ACCTTCAGCT CAGTGACAGT GAGGACAGTG ACAGTGAACA AACCCCAGAG
    AAGCCTCCCT CCTCATCTGC ACCTCCAAGT GCTCCACAGT CCCTTCCAGA ACCAGTGGCA
    TCAGCACATT CCAGCAGTGC AGAGTCAGAA AGCACCAGTG ACTCAGACAG TTCCTCAGAC
    TCAGAGAGCG AGAGCAGTTC AAGTGACAGC GAAGAAAATG AGCCCCTAGA AACCCCAGCT
    CCGGAGCCTG AGCCTCCAAC AACAAACAAA TGGCAGCTGG ACAACTGGCT GACCAAAGTC
    AGCCAGCCAG CTGCGCCACC AGAGGGCCCC AGGAGCACAG AGCCCCCACG GCGGCACCCA
    GAGAGTAAGG GCAGCAGCGA CAGTGCCACG AGTCAGGAGC ATTCTGAATC CAAAGATCCT
    CCCCCTAAAA GCTCCAGCAA AGCCCCCCGG GCCCCACCCG AAGCCCCCCA CCCCGGAAAG
    AGGAGCTGTC AGAAGTCTCC GGCACAGCAG GAGCCCCCAC AAAGGCAAAC CGTTGGAACC
    AAACAACCCA AAAAACCTGT CAAGGCCTCT GCCCGGGCAG GTTCACGGAC CAGCCTGCAG
    GGGGAAAGGG AGCCAGGGCT TCTTCCCTAT GGCTCCCGAG ACCAGACTTC CAAAGACAAG
    CCCAAGGTGA AGACGAAAGG ACGGCCCCGG GCCGCAGCAA GCAACGAACC CAAGCCAGCA
    GTGCCCCCCT CCAGTGAGAA GAAGAAGCAC AAGAGCTCCC TCCCTGCCCC CTCTAAGGCT
    CTCTCAGGCC CAGAACCCGC GAAGGACAAT GTGGAGGACA GGACCCCTGA GCACTTTGCT
    CTTGTTCCCC TGACTGAGAG CCAGGGCCCA CCCCACAGTG GCAGCGGCAG CAGGACTAGT
    GGCTGCCGCC AAGCCGTGGT GGTCCAGGAG GACAGCCGCA AAGACAGACT CCCATTGCCT
    TTGAGAGACA CCAAGCTGCT CTCACCGCTC AGGGACACTC CTCCCCCACA AAGCTTGATG
    GTGAAGATCA CCCTAGACCT GCTCTCTCGG ATACCCCAGC CTCCCGGGAA GGGGAGCCGC
    CAGAGGAAAG CAGAAGATAA ACAGCCGCCC GCAGGGAAGA AGCACAGCTC TGAGAAGAGG
    AGCTCAGACA GCTCAAGCAA GTTGGCCAAA AAGAGAAAGG GTGAAGCAGA AAGAGACTGT
    GATAACAAGA AAATCAGACT GGAGAAGGAA ATCAAATCAC AGTCATCTTC ATCTTCATCC
    TCCCACAAAG AATCTTCTAA AACAAAGCCC TCCAGGCCCT CCTCACAGTC CTCAAAGAAG
    GAAATGCTCC CCCCGCCACC CGTGTCCTCG TCCTCCCAGA AGCCAGCCAA GCCTGCACTT
    AAGAGGTCAA GGCGGGAAGC AGACACCTGT GGCCAGGACC CTCCCAAAAG TGCCAGCAGT
    ACCAAGAGCA ACCACAAAGA CTCTTCCATT CCCAAGCAGA GAAGAGTAGA GGGGAAGGGC
    TCCAGAAGCT CCTCGGAGCA CAAGGGTTCT TCCGGAGATA CTGCAAATCC TTTTCCAGTG
    CCTTCTTTGC CAAATGGTAA CTCTAAACCA GGGAAGCCTC AAGTGAAGTT TGACAAACAA
    CAAGCAGACC TTCACATGAG GGAGGCAAAA AAGATGAAGC AGAAAGCAGA GTTAATGACG
    GACAGGGTTG GAAAGGCTTT TAAGTACCTG GAAGCCGTCT TGTCCTTCAT TGAGTGCGGA
    ATTGCCACAG AGTCTGAAAG CCAGTCATCC AAGTCAGCTT ACTCTGTCTA CTCAGAAACT
    GTAGATCTCA TTAAATTCAT AATGTCATTA AAATCCTTCT CAGATGCCAC AGCGCCAACA
    CAAGAGAAAA TATTTGCTGT TTTATGCATG CGTTGCCAGT CCATTTTGAA CATGGCGATG
    TTTCGTTGTA AAAAAGACAT AGCAATAAAG TATTCTCGTA CTCTTAATAA ACACTTCGAG
    AGTTCTTCCA AAGTCGCCCA GGCACCTTCT CCATGCATTG CAAGAAGCAC AGGCACACCA
    TCCCCTCTTT CCCCAATGCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC AAGTGCTGGC
    AGTGTGGGGA GCAGTGGGGT GGCTGCCACT ATCAGCACCC CAGTCACCAT CCAGAATATG
    ACATCTTCCT ATGTCACCAT CACATCCCAT GTTCTTACCG CCTTTGACCT TTGGGAACAG
    GCCGAGGCCC TCACGAGGAA GAATAAAGAA TTCTTTGCTC GGCTCAGCAC AAATGTGTGC
    ACCTTGGCCC TCAACAGCAG TTTGGTGGAC CTGGTGCACT ATACACGACA GGGTTTTCAG
    CAGCTACAAG AATTAACCAA AACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001300889.1
    CDS486..3035
    Translation

    Target ORF information:

    RefSeq Version NM_001313960.1
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001313960.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGACCCATT CATGGCCGCC TCCTTTGACA GCAATACATA CGCCTAGTAC AGCTGAGCCA 
    TCCAAGTTTC CTTTCCCTAC AAAGGACTCT CAGCATGTCA GTTCTGTAAC CCAAAACCAA
    AAACAATATG ATACATCTTC AAAAACTCAC TCAAATTCTC AGCAAGGAAC GTCATCCATG
    CTCGAAGACG ACCTTCAGCT CAGTGACAGT GAGGACAGTG ACAGTGAACA AACCCCAGAG
    AAGCCTCCCT CCTCATCTGC ACCTCCAAGT GCTCCACAGT CCCTTCCAGA ACCAGTGGCA
    TCAGCACATT CCAGCAGTGC AGAGTCAGAA AGCACCAGTG ACTCAGACAG TTCCTCAGAC
    TCAGAGAGCG AGAGCAGTTC AAGTGACAGC GAAGAAAATG AGCCCCTAGA AACCCCAGCT
    CCGGAGCCTG AGCCTCCAAC AACAAACAAA TGGCAGCTGG ACAACTGGCT GACCAAAGTC
    AGCCAGCCAG CTGCGCCACC AGAGGGCCCC AGGAGCACAG AGCCCCCACG GCGGCACCCA
    GAGAGTAAGG GCAGCAGCGA CAGTGCCACG AGTCAGGAGC ATTCTGAATC CAAAGATCCT
    CCCCCTAAAA GCTCCAGCAA AGCCCCCCGG GCCCCACCCG AAGCCCCCCA CCCCGGAAAG
    AGGAGCTGTC AGAAGTCTCC GGCACAGCAG GAGCCCCCAC AAAGGCAAAC CGTTGGAACC
    AAACAACCCA AAAAACCTGT CAAGGCCTCT GCCCGGGCAG GTTCACGGAC CAGCCTGCAG
    GGGGAAAGGG AGCCAGGGCT TCTTCCCTAT GGCTCCCGAG ACCAGACTTC CAAAGACAAG
    CCCAAGGTGA AGACGAAAGG ACGGCCCCGG GCCGCAGCAA GCAACGAACC CAAGCCAGCA
    GTGCCCCCCT CCAGTGAGAA GAAGAAGCAC AAGAGCTCCC TCCCTGCCCC CTCTAAGGCT
    CTCTCAGGCC CAGAACCCGC GAAGGACAAT GTGGAGGACA GGACCCCTGA GCACTTTGCT
    CTTGTTCCCC TGACTGAGAG CCAGGGCCCA CCCCACAGTG GCAGCGGCAG CAGGACTAGT
    GGCTGCCGCC AAGCCGTGGT GGTCCAGGAG GACAGCCGCA AAGACAGACT CCCATTGCCT
    TTGAGAGACA CCAAGCTGCT CTCACCGCTC AGGGACACTC CTCCCCCACA AAGCTTGATG
    GTGAAGATCA CCCTAGACCT GCTCTCTCGG ATACCCCAGC CTCCCGGGAA GGGGAGCCGC
    CAGAGGAAAG CAGAAGATAA ACAGCCGCCC GCAGGGAAGA AGCACAGCTC TGAGAAGAGG
    AGCTCAGACA GCTCAAGCAA GTTGGCCAAA AAGAGAAAGG GTGAAGCAGA AAGAGACTGT
    GATAACAAGA AAATCAGACT GGAGAAGGAA ATCAAATCAC AGTCATCTTC ATCTTCATCC
    TCCCACAAAG AATCTTCTAA AACAAAGCCC TCCAGGCCCT CCTCACAGTC CTCAAAGAAG
    GAAATGCTCC CCCCGCCACC CGTGTCCTCG TCCTCCCAGA AGCCAGCCAA GCCTGCACTT
    AAGAGGTCAA GGCGGGAAGC AGACACCTGT GGCCAGGACC CTCCCAAAAG TGCCAGCAGT
    ACCAAGAGCA ACCACAAAGA CTCTTCCATT CCCAAGCAGA GAAGAGTAGA GGGGAAGGGC
    TCCAGAAGCT CCTCGGAGCA CAAGGGTTCT TCCGGAGATA CTGCAAATCC TTTTCCAGTG
    CCTTCTTTGC CAAATGGTAA CTCTAAACCA GGGAAGCCTC AAGTGAAGTT TGACAAACAA
    CAAGCAGACC TTCACATGAG GGAGGCAAAA AAGATGAAGC AGAAAGCAGA GTTAATGACG
    GACAGGGTTG GAAAGGCTTT TAAGTACCTG GAAGCCGTCT TGTCCTTCAT TGAGTGCGGA
    ATTGCCACAG AGTCTGAAAG CCAGTCATCC AAGTCAGCTT ACTCTGTCTA CTCAGAAACT
    GTAGATCTCA TTAAATTCAT AATGTCATTA AAATCCTTCT CAGATGCCAC AGCGCCAACA
    CAAGAGAAAA TATTTGCTGT TTTATGCATG CGTTGCCAGT CCATTTTGAA CATGGCGATG
    TTTCGTTGTA AAAAAGACAT AGCAATAAAG TATTCTCGTA CTCTTAATAA ACACTTCGAG
    AGTTCTTCCA AAGTCGCCCA GGCACCTTCT CCATGCATTG CAAGAAGCAC AGGCACACCA
    TCCCCTCTTT CCCCAATGCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC AAGTGCTGGC
    AGTGTGGGGA GCAGTGGGGT GGCTGCCACT ATCAGCACCC CAGTCACCAT CCAGAATATG
    ACATCTTCCT ATGTCACCAT CACATCCCAT GTTCTTACCG CCTTTGACCT TTGGGAACAG
    GCCGAGGCCC TCACGAGGAA GAATAAAGAA TTCTTTGCTC GGCTCAGCAC AAATGTGTGC
    ACCTTGGCCC TCAACAGCAG TTTGGTGGAC CTGGTGCACT ATACACGACA GGGTTTTCAG
    CAGCTACAAG AATTAACCAA AACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59003
    Clone ID Related Accession (Same CDS sequence) XM_017008216.1 , XM_017008215.2 , NM_001313960.1 , XM_017008215.1
    Accession Version XM_017008216.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGACCCATT CATGGCCGCC TCCTTTGACA GCAATACATA CGCCTAGTAC AGCTGAGCCA 
    TCCAAGTTTC CTTTCCCTAC AAAGGACTCT CAGCATGTCA GTTCTGTAAC CCAAAACCAA
    AAACAATATG ATACATCTTC AAAAACTCAC TCAAATTCTC AGCAAGGAAC GTCATCCATG
    CTCGAAGACG ACCTTCAGCT CAGTGACAGT GAGGACAGTG ACAGTGAACA AACCCCAGAG
    AAGCCTCCCT CCTCATCTGC ACCTCCAAGT GCTCCACAGT CCCTTCCAGA ACCAGTGGCA
    TCAGCACATT CCAGCAGTGC AGAGTCAGAA AGCACCAGTG ACTCAGACAG TTCCTCAGAC
    TCAGAGAGCG AGAGCAGTTC AAGTGACAGC GAAGAAAATG AGCCCCTAGA AACCCCAGCT
    CCGGAGCCTG AGCCTCCAAC AACAAACAAA TGGCAGCTGG ACAACTGGCT GACCAAAGTC
    AGCCAGCCAG CTGCGCCACC AGAGGGCCCC AGGAGCACAG AGCCCCCACG GCGGCACCCA
    GAGAGTAAGG GCAGCAGCGA CAGTGCCACG AGTCAGGAGC ATTCTGAATC CAAAGATCCT
    CCCCCTAAAA GCTCCAGCAA AGCCCCCCGG GCCCCACCCG AAGCCCCCCA CCCCGGAAAG
    AGGAGCTGTC AGAAGTCTCC GGCACAGCAG GAGCCCCCAC AAAGGCAAAC CGTTGGAACC
    AAACAACCCA AAAAACCTGT CAAGGCCTCT GCCCGGGCAG GTTCACGGAC CAGCCTGCAG
    GGGGAAAGGG AGCCAGGGCT TCTTCCCTAT GGCTCCCGAG ACCAGACTTC CAAAGACAAG
    CCCAAGGTGA AGACGAAAGG ACGGCCCCGG GCCGCAGCAA GCAACGAACC CAAGCCAGCA
    GTGCCCCCCT CCAGTGAGAA GAAGAAGCAC AAGAGCTCCC TCCCTGCCCC CTCTAAGGCT
    CTCTCAGGCC CAGAACCCGC GAAGGACAAT GTGGAGGACA GGACCCCTGA GCACTTTGCT
    CTTGTTCCCC TGACTGAGAG CCAGGGCCCA CCCCACAGTG GCAGCGGCAG CAGGACTAGT
    GGCTGCCGCC AAGCCGTGGT GGTCCAGGAG GACAGCCGCA AAGACAGACT CCCATTGCCT
    TTGAGAGACA CCAAGCTGCT CTCACCGCTC AGGGACACTC CTCCCCCACA AAGCTTGATG
    GTGAAGATCA CCCTAGACCT GCTCTCTCGG ATACCCCAGC CTCCCGGGAA GGGGAGCCGC
    CAGAGGAAAG CAGAAGATAA ACAGCCGCCC GCAGGGAAGA AGCACAGCTC TGAGAAGAGG
    AGCTCAGACA GCTCAAGCAA GTTGGCCAAA AAGAGAAAGG GTGAAGCAGA AAGAGACTGT
    GATAACAAGA AAATCAGACT GGAGAAGGAA ATCAAATCAC AGTCATCTTC ATCTTCATCC
    TCCCACAAAG AATCTTCTAA AACAAAGCCC TCCAGGCCCT CCTCACAGTC CTCAAAGAAG
    GAAATGCTCC CCCCGCCACC CGTGTCCTCG TCCTCCCAGA AGCCAGCCAA GCCTGCACTT
    AAGAGGTCAA GGCGGGAAGC AGACACCTGT GGCCAGGACC CTCCCAAAAG TGCCAGCAGT
    ACCAAGAGCA ACCACAAAGA CTCTTCCATT CCCAAGCAGA GAAGAGTAGA GGGGAAGGGC
    TCCAGAAGCT CCTCGGAGCA CAAGGGTTCT TCCGGAGATA CTGCAAATCC TTTTCCAGTG
    CCTTCTTTGC CAAATGGTAA CTCTAAACCA GGGAAGCCTC AAGTGAAGTT TGACAAACAA
    CAAGCAGACC TTCACATGAG GGAGGCAAAA AAGATGAAGC AGAAAGCAGA GTTAATGACG
    GACAGGGTTG GAAAGGCTTT TAAGTACCTG GAAGCCGTCT TGTCCTTCAT TGAGTGCGGA
    ATTGCCACAG AGTCTGAAAG CCAGTCATCC AAGTCAGCTT ACTCTGTCTA CTCAGAAACT
    GTAGATCTCA TTAAATTCAT AATGTCATTA AAATCCTTCT CAGATGCCAC AGCGCCAACA
    CAAGAGAAAA TATTTGCTGT TTTATGCATG CGTTGCCAGT CCATTTTGAA CATGGCGATG
    TTTCGTTGTA AAAAAGACAT AGCAATAAAG TATTCTCGTA CTCTTAATAA ACACTTCGAG
    AGTTCTTCCA AAGTCGCCCA GGCACCTTCT CCATGCATTG CAAGAAGCAC AGGCACACCA
    TCCCCTCTTT CCCCAATGCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC AAGTGCTGGC
    AGTGTGGGGA GCAGTGGGGT GGCTGCCACT ATCAGCACCC CAGTCACCAT CCAGAATATG
    ACATCTTCCT ATGTCACCAT CACATCCCAT GTTCTTACCG CCTTTGACCT TTGGGAACAG
    GCCGAGGCCC TCACGAGGAA GAATAAAGAA TTCTTTGCTC GGCTCAGCAC AAATGTGTGC
    ACCTTGGCCC TCAACAGCAG TTTGGTGGAC CTGGTGCACT ATACACGACA GGGTTTTCAG
    CAGCTACAAG AATTAACCAA AACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863705.1
    CDS727..3276
    Translation

    Target ORF information:

    RefSeq Version XM_017008216.1
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008216.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGACCCATT CATGGCCGCC TCCTTTGACA GCAATACATA CGCCTAGTAC AGCTGAGCCA 
    TCCAAGTTTC CTTTCCCTAC AAAGGACTCT CAGCATGTCA GTTCTGTAAC CCAAAACCAA
    AAACAATATG ATACATCTTC AAAAACTCAC TCAAATTCTC AGCAAGGAAC GTCATCCATG
    CTCGAAGACG ACCTTCAGCT CAGTGACAGT GAGGACAGTG ACAGTGAACA AACCCCAGAG
    AAGCCTCCCT CCTCATCTGC ACCTCCAAGT GCTCCACAGT CCCTTCCAGA ACCAGTGGCA
    TCAGCACATT CCAGCAGTGC AGAGTCAGAA AGCACCAGTG ACTCAGACAG TTCCTCAGAC
    TCAGAGAGCG AGAGCAGTTC AAGTGACAGC GAAGAAAATG AGCCCCTAGA AACCCCAGCT
    CCGGAGCCTG AGCCTCCAAC AACAAACAAA TGGCAGCTGG ACAACTGGCT GACCAAAGTC
    AGCCAGCCAG CTGCGCCACC AGAGGGCCCC AGGAGCACAG AGCCCCCACG GCGGCACCCA
    GAGAGTAAGG GCAGCAGCGA CAGTGCCACG AGTCAGGAGC ATTCTGAATC CAAAGATCCT
    CCCCCTAAAA GCTCCAGCAA AGCCCCCCGG GCCCCACCCG AAGCCCCCCA CCCCGGAAAG
    AGGAGCTGTC AGAAGTCTCC GGCACAGCAG GAGCCCCCAC AAAGGCAAAC CGTTGGAACC
    AAACAACCCA AAAAACCTGT CAAGGCCTCT GCCCGGGCAG GTTCACGGAC CAGCCTGCAG
    GGGGAAAGGG AGCCAGGGCT TCTTCCCTAT GGCTCCCGAG ACCAGACTTC CAAAGACAAG
    CCCAAGGTGA AGACGAAAGG ACGGCCCCGG GCCGCAGCAA GCAACGAACC CAAGCCAGCA
    GTGCCCCCCT CCAGTGAGAA GAAGAAGCAC AAGAGCTCCC TCCCTGCCCC CTCTAAGGCT
    CTCTCAGGCC CAGAACCCGC GAAGGACAAT GTGGAGGACA GGACCCCTGA GCACTTTGCT
    CTTGTTCCCC TGACTGAGAG CCAGGGCCCA CCCCACAGTG GCAGCGGCAG CAGGACTAGT
    GGCTGCCGCC AAGCCGTGGT GGTCCAGGAG GACAGCCGCA AAGACAGACT CCCATTGCCT
    TTGAGAGACA CCAAGCTGCT CTCACCGCTC AGGGACACTC CTCCCCCACA AAGCTTGATG
    GTGAAGATCA CCCTAGACCT GCTCTCTCGG ATACCCCAGC CTCCCGGGAA GGGGAGCCGC
    CAGAGGAAAG CAGAAGATAA ACAGCCGCCC GCAGGGAAGA AGCACAGCTC TGAGAAGAGG
    AGCTCAGACA GCTCAAGCAA GTTGGCCAAA AAGAGAAAGG GTGAAGCAGA AAGAGACTGT
    GATAACAAGA AAATCAGACT GGAGAAGGAA ATCAAATCAC AGTCATCTTC ATCTTCATCC
    TCCCACAAAG AATCTTCTAA AACAAAGCCC TCCAGGCCCT CCTCACAGTC CTCAAAGAAG
    GAAATGCTCC CCCCGCCACC CGTGTCCTCG TCCTCCCAGA AGCCAGCCAA GCCTGCACTT
    AAGAGGTCAA GGCGGGAAGC AGACACCTGT GGCCAGGACC CTCCCAAAAG TGCCAGCAGT
    ACCAAGAGCA ACCACAAAGA CTCTTCCATT CCCAAGCAGA GAAGAGTAGA GGGGAAGGGC
    TCCAGAAGCT CCTCGGAGCA CAAGGGTTCT TCCGGAGATA CTGCAAATCC TTTTCCAGTG
    CCTTCTTTGC CAAATGGTAA CTCTAAACCA GGGAAGCCTC AAGTGAAGTT TGACAAACAA
    CAAGCAGACC TTCACATGAG GGAGGCAAAA AAGATGAAGC AGAAAGCAGA GTTAATGACG
    GACAGGGTTG GAAAGGCTTT TAAGTACCTG GAAGCCGTCT TGTCCTTCAT TGAGTGCGGA
    ATTGCCACAG AGTCTGAAAG CCAGTCATCC AAGTCAGCTT ACTCTGTCTA CTCAGAAACT
    GTAGATCTCA TTAAATTCAT AATGTCATTA AAATCCTTCT CAGATGCCAC AGCGCCAACA
    CAAGAGAAAA TATTTGCTGT TTTATGCATG CGTTGCCAGT CCATTTTGAA CATGGCGATG
    TTTCGTTGTA AAAAAGACAT AGCAATAAAG TATTCTCGTA CTCTTAATAA ACACTTCGAG
    AGTTCTTCCA AAGTCGCCCA GGCACCTTCT CCATGCATTG CAAGAAGCAC AGGCACACCA
    TCCCCTCTTT CCCCAATGCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC AAGTGCTGGC
    AGTGTGGGGA GCAGTGGGGT GGCTGCCACT ATCAGCACCC CAGTCACCAT CCAGAATATG
    ACATCTTCCT ATGTCACCAT CACATCCCAT GTTCTTACCG CCTTTGACCT TTGGGAACAG
    GCCGAGGCCC TCACGAGGAA GAATAAAGAA TTCTTTGCTC GGCTCAGCAC AAATGTGTGC
    ACCTTGGCCC TCAACAGCAG TTTGGTGGAC CTGGTGCACT ATACACGACA GGGTTTTCAG
    CAGCTACAAG AATTAACCAA AACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59003
    Clone ID Related Accession (Same CDS sequence) XM_017008216.1 , XM_017008215.2 , NM_001313960.1 , XM_017008215.1
    Accession Version XM_017008215.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008215.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGACCCATT CATGGCCGCC TCCTTTGACA GCAATACATA CGCCTAGTAC AGCTGAGCCA 
    TCCAAGTTTC CTTTCCCTAC AAAGGACTCT CAGCATGTCA GTTCTGTAAC CCAAAACCAA
    AAACAATATG ATACATCTTC AAAAACTCAC TCAAATTCTC AGCAAGGAAC GTCATCCATG
    CTCGAAGACG ACCTTCAGCT CAGTGACAGT GAGGACAGTG ACAGTGAACA AACCCCAGAG
    AAGCCTCCCT CCTCATCTGC ACCTCCAAGT GCTCCACAGT CCCTTCCAGA ACCAGTGGCA
    TCAGCACATT CCAGCAGTGC AGAGTCAGAA AGCACCAGTG ACTCAGACAG TTCCTCAGAC
    TCAGAGAGCG AGAGCAGTTC AAGTGACAGC GAAGAAAATG AGCCCCTAGA AACCCCAGCT
    CCGGAGCCTG AGCCTCCAAC AACAAACAAA TGGCAGCTGG ACAACTGGCT GACCAAAGTC
    AGCCAGCCAG CTGCGCCACC AGAGGGCCCC AGGAGCACAG AGCCCCCACG GCGGCACCCA
    GAGAGTAAGG GCAGCAGCGA CAGTGCCACG AGTCAGGAGC ATTCTGAATC CAAAGATCCT
    CCCCCTAAAA GCTCCAGCAA AGCCCCCCGG GCCCCACCCG AAGCCCCCCA CCCCGGAAAG
    AGGAGCTGTC AGAAGTCTCC GGCACAGCAG GAGCCCCCAC AAAGGCAAAC CGTTGGAACC
    AAACAACCCA AAAAACCTGT CAAGGCCTCT GCCCGGGCAG GTTCACGGAC CAGCCTGCAG
    GGGGAAAGGG AGCCAGGGCT TCTTCCCTAT GGCTCCCGAG ACCAGACTTC CAAAGACAAG
    CCCAAGGTGA AGACGAAAGG ACGGCCCCGG GCCGCAGCAA GCAACGAACC CAAGCCAGCA
    GTGCCCCCCT CCAGTGAGAA GAAGAAGCAC AAGAGCTCCC TCCCTGCCCC CTCTAAGGCT
    CTCTCAGGCC CAGAACCCGC GAAGGACAAT GTGGAGGACA GGACCCCTGA GCACTTTGCT
    CTTGTTCCCC TGACTGAGAG CCAGGGCCCA CCCCACAGTG GCAGCGGCAG CAGGACTAGT
    GGCTGCCGCC AAGCCGTGGT GGTCCAGGAG GACAGCCGCA AAGACAGACT CCCATTGCCT
    TTGAGAGACA CCAAGCTGCT CTCACCGCTC AGGGACACTC CTCCCCCACA AAGCTTGATG
    GTGAAGATCA CCCTAGACCT GCTCTCTCGG ATACCCCAGC CTCCCGGGAA GGGGAGCCGC
    CAGAGGAAAG CAGAAGATAA ACAGCCGCCC GCAGGGAAGA AGCACAGCTC TGAGAAGAGG
    AGCTCAGACA GCTCAAGCAA GTTGGCCAAA AAGAGAAAGG GTGAAGCAGA AAGAGACTGT
    GATAACAAGA AAATCAGACT GGAGAAGGAA ATCAAATCAC AGTCATCTTC ATCTTCATCC
    TCCCACAAAG AATCTTCTAA AACAAAGCCC TCCAGGCCCT CCTCACAGTC CTCAAAGAAG
    GAAATGCTCC CCCCGCCACC CGTGTCCTCG TCCTCCCAGA AGCCAGCCAA GCCTGCACTT
    AAGAGGTCAA GGCGGGAAGC AGACACCTGT GGCCAGGACC CTCCCAAAAG TGCCAGCAGT
    ACCAAGAGCA ACCACAAAGA CTCTTCCATT CCCAAGCAGA GAAGAGTAGA GGGGAAGGGC
    TCCAGAAGCT CCTCGGAGCA CAAGGGTTCT TCCGGAGATA CTGCAAATCC TTTTCCAGTG
    CCTTCTTTGC CAAATGGTAA CTCTAAACCA GGGAAGCCTC AAGTGAAGTT TGACAAACAA
    CAAGCAGACC TTCACATGAG GGAGGCAAAA AAGATGAAGC AGAAAGCAGA GTTAATGACG
    GACAGGGTTG GAAAGGCTTT TAAGTACCTG GAAGCCGTCT TGTCCTTCAT TGAGTGCGGA
    ATTGCCACAG AGTCTGAAAG CCAGTCATCC AAGTCAGCTT ACTCTGTCTA CTCAGAAACT
    GTAGATCTCA TTAAATTCAT AATGTCATTA AAATCCTTCT CAGATGCCAC AGCGCCAACA
    CAAGAGAAAA TATTTGCTGT TTTATGCATG CGTTGCCAGT CCATTTTGAA CATGGCGATG
    TTTCGTTGTA AAAAAGACAT AGCAATAAAG TATTCTCGTA CTCTTAATAA ACACTTCGAG
    AGTTCTTCCA AAGTCGCCCA GGCACCTTCT CCATGCATTG CAAGAAGCAC AGGCACACCA
    TCCCCTCTTT CCCCAATGCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC AAGTGCTGGC
    AGTGTGGGGA GCAGTGGGGT GGCTGCCACT ATCAGCACCC CAGTCACCAT CCAGAATATG
    ACATCTTCCT ATGTCACCAT CACATCCCAT GTTCTTACCG CCTTTGACCT TTGGGAACAG
    GCCGAGGCCC TCACGAGGAA GAATAAAGAA TTCTTTGCTC GGCTCAGCAC AAATGTGTGC
    ACCTTGGCCC TCAACAGCAG TTTGGTGGAC CTGGTGCACT ATACACGACA GGGTTTTCAG
    CAGCTACAAG AATTAACCAA AACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863704.1
    CDS758..3307
    Translation

    Target ORF information:

    RefSeq Version XM_017008215.2
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008215.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGACCCATT CATGGCCGCC TCCTTTGACA GCAATACATA CGCCTAGTAC AGCTGAGCCA 
    TCCAAGTTTC CTTTCCCTAC AAAGGACTCT CAGCATGTCA GTTCTGTAAC CCAAAACCAA
    AAACAATATG ATACATCTTC AAAAACTCAC TCAAATTCTC AGCAAGGAAC GTCATCCATG
    CTCGAAGACG ACCTTCAGCT CAGTGACAGT GAGGACAGTG ACAGTGAACA AACCCCAGAG
    AAGCCTCCCT CCTCATCTGC ACCTCCAAGT GCTCCACAGT CCCTTCCAGA ACCAGTGGCA
    TCAGCACATT CCAGCAGTGC AGAGTCAGAA AGCACCAGTG ACTCAGACAG TTCCTCAGAC
    TCAGAGAGCG AGAGCAGTTC AAGTGACAGC GAAGAAAATG AGCCCCTAGA AACCCCAGCT
    CCGGAGCCTG AGCCTCCAAC AACAAACAAA TGGCAGCTGG ACAACTGGCT GACCAAAGTC
    AGCCAGCCAG CTGCGCCACC AGAGGGCCCC AGGAGCACAG AGCCCCCACG GCGGCACCCA
    GAGAGTAAGG GCAGCAGCGA CAGTGCCACG AGTCAGGAGC ATTCTGAATC CAAAGATCCT
    CCCCCTAAAA GCTCCAGCAA AGCCCCCCGG GCCCCACCCG AAGCCCCCCA CCCCGGAAAG
    AGGAGCTGTC AGAAGTCTCC GGCACAGCAG GAGCCCCCAC AAAGGCAAAC CGTTGGAACC
    AAACAACCCA AAAAACCTGT CAAGGCCTCT GCCCGGGCAG GTTCACGGAC CAGCCTGCAG
    GGGGAAAGGG AGCCAGGGCT TCTTCCCTAT GGCTCCCGAG ACCAGACTTC CAAAGACAAG
    CCCAAGGTGA AGACGAAAGG ACGGCCCCGG GCCGCAGCAA GCAACGAACC CAAGCCAGCA
    GTGCCCCCCT CCAGTGAGAA GAAGAAGCAC AAGAGCTCCC TCCCTGCCCC CTCTAAGGCT
    CTCTCAGGCC CAGAACCCGC GAAGGACAAT GTGGAGGACA GGACCCCTGA GCACTTTGCT
    CTTGTTCCCC TGACTGAGAG CCAGGGCCCA CCCCACAGTG GCAGCGGCAG CAGGACTAGT
    GGCTGCCGCC AAGCCGTGGT GGTCCAGGAG GACAGCCGCA AAGACAGACT CCCATTGCCT
    TTGAGAGACA CCAAGCTGCT CTCACCGCTC AGGGACACTC CTCCCCCACA AAGCTTGATG
    GTGAAGATCA CCCTAGACCT GCTCTCTCGG ATACCCCAGC CTCCCGGGAA GGGGAGCCGC
    CAGAGGAAAG CAGAAGATAA ACAGCCGCCC GCAGGGAAGA AGCACAGCTC TGAGAAGAGG
    AGCTCAGACA GCTCAAGCAA GTTGGCCAAA AAGAGAAAGG GTGAAGCAGA AAGAGACTGT
    GATAACAAGA AAATCAGACT GGAGAAGGAA ATCAAATCAC AGTCATCTTC ATCTTCATCC
    TCCCACAAAG AATCTTCTAA AACAAAGCCC TCCAGGCCCT CCTCACAGTC CTCAAAGAAG
    GAAATGCTCC CCCCGCCACC CGTGTCCTCG TCCTCCCAGA AGCCAGCCAA GCCTGCACTT
    AAGAGGTCAA GGCGGGAAGC AGACACCTGT GGCCAGGACC CTCCCAAAAG TGCCAGCAGT
    ACCAAGAGCA ACCACAAAGA CTCTTCCATT CCCAAGCAGA GAAGAGTAGA GGGGAAGGGC
    TCCAGAAGCT CCTCGGAGCA CAAGGGTTCT TCCGGAGATA CTGCAAATCC TTTTCCAGTG
    CCTTCTTTGC CAAATGGTAA CTCTAAACCA GGGAAGCCTC AAGTGAAGTT TGACAAACAA
    CAAGCAGACC TTCACATGAG GGAGGCAAAA AAGATGAAGC AGAAAGCAGA GTTAATGACG
    GACAGGGTTG GAAAGGCTTT TAAGTACCTG GAAGCCGTCT TGTCCTTCAT TGAGTGCGGA
    ATTGCCACAG AGTCTGAAAG CCAGTCATCC AAGTCAGCTT ACTCTGTCTA CTCAGAAACT
    GTAGATCTCA TTAAATTCAT AATGTCATTA AAATCCTTCT CAGATGCCAC AGCGCCAACA
    CAAGAGAAAA TATTTGCTGT TTTATGCATG CGTTGCCAGT CCATTTTGAA CATGGCGATG
    TTTCGTTGTA AAAAAGACAT AGCAATAAAG TATTCTCGTA CTCTTAATAA ACACTTCGAG
    AGTTCTTCCA AAGTCGCCCA GGCACCTTCT CCATGCATTG CAAGAAGCAC AGGCACACCA
    TCCCCTCTTT CCCCAATGCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC AAGTGCTGGC
    AGTGTGGGGA GCAGTGGGGT GGCTGCCACT ATCAGCACCC CAGTCACCAT CCAGAATATG
    ACATCTTCCT ATGTCACCAT CACATCCCAT GTTCTTACCG CCTTTGACCT TTGGGAACAG
    GCCGAGGCCC TCACGAGGAA GAATAAAGAA TTCTTTGCTC GGCTCAGCAC AAATGTGTGC
    ACCTTGGCCC TCAACAGCAG TTTGGTGGAC CTGGTGCACT ATACACGACA GGGTTTTCAG
    CAGCTACAAG AATTAACCAA AACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59003
    Clone ID Related Accession (Same CDS sequence) XM_017008216.1 , XM_017008215.2 , NM_001313960.1 , XM_017008215.1
    Accession Version XM_017008215.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGACCCATT CATGGCCGCC TCCTTTGACA GCAATACATA CGCCTAGTAC AGCTGAGCCA 
    TCCAAGTTTC CTTTCCCTAC AAAGGACTCT CAGCATGTCA GTTCTGTAAC CCAAAACCAA
    AAACAATATG ATACATCTTC AAAAACTCAC TCAAATTCTC AGCAAGGAAC GTCATCCATG
    CTCGAAGACG ACCTTCAGCT CAGTGACAGT GAGGACAGTG ACAGTGAACA AACCCCAGAG
    AAGCCTCCCT CCTCATCTGC ACCTCCAAGT GCTCCACAGT CCCTTCCAGA ACCAGTGGCA
    TCAGCACATT CCAGCAGTGC AGAGTCAGAA AGCACCAGTG ACTCAGACAG TTCCTCAGAC
    TCAGAGAGCG AGAGCAGTTC AAGTGACAGC GAAGAAAATG AGCCCCTAGA AACCCCAGCT
    CCGGAGCCTG AGCCTCCAAC AACAAACAAA TGGCAGCTGG ACAACTGGCT GACCAAAGTC
    AGCCAGCCAG CTGCGCCACC AGAGGGCCCC AGGAGCACAG AGCCCCCACG GCGGCACCCA
    GAGAGTAAGG GCAGCAGCGA CAGTGCCACG AGTCAGGAGC ATTCTGAATC CAAAGATCCT
    CCCCCTAAAA GCTCCAGCAA AGCCCCCCGG GCCCCACCCG AAGCCCCCCA CCCCGGAAAG
    AGGAGCTGTC AGAAGTCTCC GGCACAGCAG GAGCCCCCAC AAAGGCAAAC CGTTGGAACC
    AAACAACCCA AAAAACCTGT CAAGGCCTCT GCCCGGGCAG GTTCACGGAC CAGCCTGCAG
    GGGGAAAGGG AGCCAGGGCT TCTTCCCTAT GGCTCCCGAG ACCAGACTTC CAAAGACAAG
    CCCAAGGTGA AGACGAAAGG ACGGCCCCGG GCCGCAGCAA GCAACGAACC CAAGCCAGCA
    GTGCCCCCCT CCAGTGAGAA GAAGAAGCAC AAGAGCTCCC TCCCTGCCCC CTCTAAGGCT
    CTCTCAGGCC CAGAACCCGC GAAGGACAAT GTGGAGGACA GGACCCCTGA GCACTTTGCT
    CTTGTTCCCC TGACTGAGAG CCAGGGCCCA CCCCACAGTG GCAGCGGCAG CAGGACTAGT
    GGCTGCCGCC AAGCCGTGGT GGTCCAGGAG GACAGCCGCA AAGACAGACT CCCATTGCCT
    TTGAGAGACA CCAAGCTGCT CTCACCGCTC AGGGACACTC CTCCCCCACA AAGCTTGATG
    GTGAAGATCA CCCTAGACCT GCTCTCTCGG ATACCCCAGC CTCCCGGGAA GGGGAGCCGC
    CAGAGGAAAG CAGAAGATAA ACAGCCGCCC GCAGGGAAGA AGCACAGCTC TGAGAAGAGG
    AGCTCAGACA GCTCAAGCAA GTTGGCCAAA AAGAGAAAGG GTGAAGCAGA AAGAGACTGT
    GATAACAAGA AAATCAGACT GGAGAAGGAA ATCAAATCAC AGTCATCTTC ATCTTCATCC
    TCCCACAAAG AATCTTCTAA AACAAAGCCC TCCAGGCCCT CCTCACAGTC CTCAAAGAAG
    GAAATGCTCC CCCCGCCACC CGTGTCCTCG TCCTCCCAGA AGCCAGCCAA GCCTGCACTT
    AAGAGGTCAA GGCGGGAAGC AGACACCTGT GGCCAGGACC CTCCCAAAAG TGCCAGCAGT
    ACCAAGAGCA ACCACAAAGA CTCTTCCATT CCCAAGCAGA GAAGAGTAGA GGGGAAGGGC
    TCCAGAAGCT CCTCGGAGCA CAAGGGTTCT TCCGGAGATA CTGCAAATCC TTTTCCAGTG
    CCTTCTTTGC CAAATGGTAA CTCTAAACCA GGGAAGCCTC AAGTGAAGTT TGACAAACAA
    CAAGCAGACC TTCACATGAG GGAGGCAAAA AAGATGAAGC AGAAAGCAGA GTTAATGACG
    GACAGGGTTG GAAAGGCTTT TAAGTACCTG GAAGCCGTCT TGTCCTTCAT TGAGTGCGGA
    ATTGCCACAG AGTCTGAAAG CCAGTCATCC AAGTCAGCTT ACTCTGTCTA CTCAGAAACT
    GTAGATCTCA TTAAATTCAT AATGTCATTA AAATCCTTCT CAGATGCCAC AGCGCCAACA
    CAAGAGAAAA TATTTGCTGT TTTATGCATG CGTTGCCAGT CCATTTTGAA CATGGCGATG
    TTTCGTTGTA AAAAAGACAT AGCAATAAAG TATTCTCGTA CTCTTAATAA ACACTTCGAG
    AGTTCTTCCA AAGTCGCCCA GGCACCTTCT CCATGCATTG CAAGAAGCAC AGGCACACCA
    TCCCCTCTTT CCCCAATGCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC AAGTGCTGGC
    AGTGTGGGGA GCAGTGGGGT GGCTGCCACT ATCAGCACCC CAGTCACCAT CCAGAATATG
    ACATCTTCCT ATGTCACCAT CACATCCCAT GTTCTTACCG CCTTTGACCT TTGGGAACAG
    GCCGAGGCCC TCACGAGGAA GAATAAAGAA TTCTTTGCTC GGCTCAGCAC AAATGTGTGC
    ACCTTGGCCC TCAACAGCAG TTTGGTGGAC CTGGTGCACT ATACACGACA GGGTTTTCAG
    CAGCTACAAG AATTAACCAA AACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863704.1
    CDS340..2889
    Translation

    Target ORF information:

    RefSeq Version XM_017008215.1
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008215.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGACCCATT CATGGCCGCC TCCTTTGACA GCAATACATA CGCCTAGTAC AGCTGAGCCA 
    TCCAAGTTTC CTTTCCCTAC AAAGGACTCT CAGCATGTCA GTTCTGTAAC CCAAAACCAA
    AAACAATATG ATACATCTTC AAAAACTCAC TCAAATTCTC AGCAAGGAAC GTCATCCATG
    CTCGAAGACG ACCTTCAGCT CAGTGACAGT GAGGACAGTG ACAGTGAACA AACCCCAGAG
    AAGCCTCCCT CCTCATCTGC ACCTCCAAGT GCTCCACAGT CCCTTCCAGA ACCAGTGGCA
    TCAGCACATT CCAGCAGTGC AGAGTCAGAA AGCACCAGTG ACTCAGACAG TTCCTCAGAC
    TCAGAGAGCG AGAGCAGTTC AAGTGACAGC GAAGAAAATG AGCCCCTAGA AACCCCAGCT
    CCGGAGCCTG AGCCTCCAAC AACAAACAAA TGGCAGCTGG ACAACTGGCT GACCAAAGTC
    AGCCAGCCAG CTGCGCCACC AGAGGGCCCC AGGAGCACAG AGCCCCCACG GCGGCACCCA
    GAGAGTAAGG GCAGCAGCGA CAGTGCCACG AGTCAGGAGC ATTCTGAATC CAAAGATCCT
    CCCCCTAAAA GCTCCAGCAA AGCCCCCCGG GCCCCACCCG AAGCCCCCCA CCCCGGAAAG
    AGGAGCTGTC AGAAGTCTCC GGCACAGCAG GAGCCCCCAC AAAGGCAAAC CGTTGGAACC
    AAACAACCCA AAAAACCTGT CAAGGCCTCT GCCCGGGCAG GTTCACGGAC CAGCCTGCAG
    GGGGAAAGGG AGCCAGGGCT TCTTCCCTAT GGCTCCCGAG ACCAGACTTC CAAAGACAAG
    CCCAAGGTGA AGACGAAAGG ACGGCCCCGG GCCGCAGCAA GCAACGAACC CAAGCCAGCA
    GTGCCCCCCT CCAGTGAGAA GAAGAAGCAC AAGAGCTCCC TCCCTGCCCC CTCTAAGGCT
    CTCTCAGGCC CAGAACCCGC GAAGGACAAT GTGGAGGACA GGACCCCTGA GCACTTTGCT
    CTTGTTCCCC TGACTGAGAG CCAGGGCCCA CCCCACAGTG GCAGCGGCAG CAGGACTAGT
    GGCTGCCGCC AAGCCGTGGT GGTCCAGGAG GACAGCCGCA AAGACAGACT CCCATTGCCT
    TTGAGAGACA CCAAGCTGCT CTCACCGCTC AGGGACACTC CTCCCCCACA AAGCTTGATG
    GTGAAGATCA CCCTAGACCT GCTCTCTCGG ATACCCCAGC CTCCCGGGAA GGGGAGCCGC
    CAGAGGAAAG CAGAAGATAA ACAGCCGCCC GCAGGGAAGA AGCACAGCTC TGAGAAGAGG
    AGCTCAGACA GCTCAAGCAA GTTGGCCAAA AAGAGAAAGG GTGAAGCAGA AAGAGACTGT
    GATAACAAGA AAATCAGACT GGAGAAGGAA ATCAAATCAC AGTCATCTTC ATCTTCATCC
    TCCCACAAAG AATCTTCTAA AACAAAGCCC TCCAGGCCCT CCTCACAGTC CTCAAAGAAG
    GAAATGCTCC CCCCGCCACC CGTGTCCTCG TCCTCCCAGA AGCCAGCCAA GCCTGCACTT
    AAGAGGTCAA GGCGGGAAGC AGACACCTGT GGCCAGGACC CTCCCAAAAG TGCCAGCAGT
    ACCAAGAGCA ACCACAAAGA CTCTTCCATT CCCAAGCAGA GAAGAGTAGA GGGGAAGGGC
    TCCAGAAGCT CCTCGGAGCA CAAGGGTTCT TCCGGAGATA CTGCAAATCC TTTTCCAGTG
    CCTTCTTTGC CAAATGGTAA CTCTAAACCA GGGAAGCCTC AAGTGAAGTT TGACAAACAA
    CAAGCAGACC TTCACATGAG GGAGGCAAAA AAGATGAAGC AGAAAGCAGA GTTAATGACG
    GACAGGGTTG GAAAGGCTTT TAAGTACCTG GAAGCCGTCT TGTCCTTCAT TGAGTGCGGA
    ATTGCCACAG AGTCTGAAAG CCAGTCATCC AAGTCAGCTT ACTCTGTCTA CTCAGAAACT
    GTAGATCTCA TTAAATTCAT AATGTCATTA AAATCCTTCT CAGATGCCAC AGCGCCAACA
    CAAGAGAAAA TATTTGCTGT TTTATGCATG CGTTGCCAGT CCATTTTGAA CATGGCGATG
    TTTCGTTGTA AAAAAGACAT AGCAATAAAG TATTCTCGTA CTCTTAATAA ACACTTCGAG
    AGTTCTTCCA AAGTCGCCCA GGCACCTTCT CCATGCATTG CAAGAAGCAC AGGCACACCA
    TCCCCTCTTT CCCCAATGCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC AAGTGCTGGC
    AGTGTGGGGA GCAGTGGGGT GGCTGCCACT ATCAGCACCC CAGTCACCAT CCAGAATATG
    ACATCTTCCT ATGTCACCAT CACATCCCAT GTTCTTACCG CCTTTGACCT TTGGGAACAG
    GCCGAGGCCC TCACGAGGAA GAATAAAGAA TTCTTTGCTC GGCTCAGCAC AAATGTGTGC
    ACCTTGGCCC TCAACAGCAG TTTGGTGGAC CTGGTGCACT ATACACGACA GGGTTTTCAG
    CAGCTACAAG AATTAACCAA AACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24271
    Clone ID Related Accession (Same CDS sequence) NM_005935.3
    Accession Version NM_005935.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L13773.1, BC114930.1, AC092658.2 and AI638134.1. On Sep 16, 2015 this sequence version replaced NM_005935.2. Transcript Variant: This variant (2) contains a distinct 5' UTR, uses an alternate translation start site, and uses an alternate in-frame splice site in the 3' coding region compared to variant 1. It encodes isoform 2, which has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L13773.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAGCCC AGTCAAGTTT GTACAATGAC GACAGAAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC CCACCAAGAG AAAGAGGCAT TTCCTGAAAA GATTCCCCTT
    TTTGGAGAGC CCTACAAGAC AGCAAAAGGT GATGAGCTGT CTAGTCGAAT ACAGAACATG
    TTGGGAAACT ACGAAGAAGT GAAGGAGTTC CTTAGTACTA AGTCTCACAC TCATCGCCTG
    GATGCTTCTG AAAATAGGTT GGGAAAGCCG AAATATCCTT TAATTCCTGA CAAAGGGAGC
    AGCATTCCAT CCAGCTCCTT CCACACTAGT GTCCACCACC AGTCCATTCA CACTCCTGCG
    TCTGGACCAC TTTCTGTTGG CAACATTAGC CACAATCCAA AGATGGCGCA GCCAAGAACT
    GAACCAATGC CAAGTCTCCA TGCCAAAAGC TGCGGCCCAC CGGACAGCCA GCACCTGACC
    CAGGATCGCC TTGGTCAGGA GGGGTTCGGC TCTAGTCATC ACAAGAAAGG TGACCGAAGA
    GCTGACGGAG ACCACTGTGC TTCGGTGACA GATTCGGCTC CAGAGAGGGA GCTTTCTCCC
    TTAATCTCTT TGCCTTCCCC AGTTCCCCCT TTGTCACCTA TACATTCCAA CCAGCAAACT
    CTTCCCCGGA CGCAAGGAAG CAGCAAGGTT CATGGCAGCA GCAATAACAG TAAAGGCTAT
    TGCCCAGCCA AATCTCCCAA GGACCTAGCA GTGAAAGTCC ATGATAAAGA GACCCCTCAA
    GACAGTTTGG TGGCCCCTGC CCAGCCGCCT TCTCAGACAT TTCCACCTCC CTCCCTCCCC
    TCAAAAAGTG TTGCAATGCA GCAGAAGCCC ACGGCTTATG TCCGGCCCAT GGATGGTCAA
    GATCAGGCCC CTAGTGAATC CCCTGAACTG AAACCACTGC CGGAGGACTA TCGACAGCAG
    ACCTTTGAAA AAACAGACTT GAAAGTGCCT GCCAAAGCCA AGCTCACCAA ACTGAAGATG
    CCTTCTCAGT CAGTTGAGCA GACCTACTCC AATGAAGTCC ATTGTGTTGA AGAGATTCTG
    AAGGAAATGA CCCATTCATG GCCGCCTCCT TTGACAGCAA TACATACGCC TAGTACAGCT
    GAGCCATCCA AGTTTCCTTT CCCTACAAAG GACTCTCAGC ATGTCAGTTC TGTAACCCAA
    AACCAAAAAC AATATGATAC ATCTTCAAAA ACTCACTCAA ATTCTCAGCA AGGAACGTCA
    TCCATGCTCG AAGACGACCT TCAGCTCAGT GACAGTGAGG ACAGTGACAG TGAACAAACC
    CCAGAGAAGC CTCCCTCCTC ATCTGCACCT CCAAGTGCTC CACAGTCCCT TCCAGAACCA
    GTGGCATCAG CACATTCCAG CAGTGCAGAG TCAGAAAGCA CCAGTGACTC AGACAGTTCC
    TCAGACTCAG AGAGCGAGAG CAGTTCAAGT GACAGCGAAG AAAATGAGCC CCTAGAAACC
    CCAGCTCCGG AGCCTGAGCC TCCAACAACA AACAAATGGC AGCTGGACAA CTGGCTGACC
    AAAGTCAGCC AGCCAGCTGC GCCACCAGAG GGCCCCAGGA GCACAGAGCC CCCACGGCGG
    CACCCAGAGA GTAAGGGCAG CAGCGACAGT GCCACGAGTC AGGAGCATTC TGAATCCAAA
    GATCCTCCCC CTAAAAGCTC CAGCAAAGCC CCCCGGGCCC CACCCGAAGC CCCCCACCCC
    GGAAAGAGGA GCTGTCAGAA GTCTCCGGCA CAGCAGGAGC CCCCACAAAG GCAAACCGTT
    GGAACCAAAC AACCCAAAAA ACCTGTCAAG GCCTCTGCCC GGGCAGGTTC ACGGACCAGC
    CTGCAGGGGG AAAGGGAGCC AGGGCTTCTT CCCTATGGCT CCCGAGACCA GACTTCCAAA
    GACAAGCCCA AGGTGAAGAC GAAAGGACGG CCCCGGGCCG CAGCAAGCAA CGAACCCAAG
    CCAGCAGTGC CCCCCTCCAG TGAGAAGAAG AAGCACAAGA GCTCCCTCCC TGCCCCCTCT
    AAGGCTCTCT CAGGCCCAGA ACCCGCGAAG GACAATGTGG AGGACAGGAC CCCTGAGCAC
    TTTGCTCTTG TTCCCCTGAC TGAGAGCCAG GGCCCACCCC ACAGTGGCAG CGGCAGCAGG
    ACTAGTGGCT GCCGCCAAGC CGTGGTGGTC CAGGAGGACA GCCGCAAAGA CAGACTCCCA
    TTGCCTTTGA GAGACACCAA GCTGCTCTCA CCGCTCAGGG ACACTCCTCC CCCACAAAGC
    TTGATGGTGA AGATCACCCT AGACCTGCTC TCTCGGATAC CCCAGCCTCC CGGGAAGGGG
    AGCCGCCAGA GGAAAGCAGA AGATAAACAG CCGCCCGCAG GGAAGAAGCA CAGCTCTGAG
    AAGAGGAGCT CAGACAGCTC AAGCAAGTTG GCCAAAAAGA GAAAGGGTGA AGCAGAAAGA
    GACTGTGATA ACAAGAAAAT CAGACTGGAG AAGGAAATCA AATCACAGTC ATCTTCATCT
    TCATCCTCCC ACAAAGAATC TTCTAAAACA AAGCCCTCCA GGCCCTCCTC ACAGTCCTCA
    AAGAAGGAAA TGCTCCCCCC GCCACCCGTG TCCTCGTCCT CCCAGAAGCC AGCCAAGCCT
    GCACTTAAGA GGTCAAGGCG GGAAGCAGAC ACCTGTGGCC AGGACCCTCC CAAAAGTGCC
    AGCAGTACCA AGAGCAACCA CAAAGACTCT TCCATTCCCA AGCAGAGAAG AGTAGAGGGG
    AAGGGCTCCA GAAGCTCCTC GGAGCACAAG GGTTCTTCCG GAGATACTGC AAATCCTTTT
    CCAGTGCCTT CTTTGCCAAA TGGTAACTCT AAACCAGGGA AGCCTCAAGT GAAGTTTGAC
    AAACAACAAG CAGACCTTCA CATGAGGGAG GCAAAAAAGA TGAAGCAGAA AGCAGAGTTA
    ATGACGGACA GGGTTGGAAA GGCTTTTAAG TACCTGGAAG CCGTCTTGTC CTTCATTGAG
    TGCGGAATTG CCACAGAGTC TGAAAGCCAG TCATCCAAGT CAGCTTACTC TGTCTACTCA
    GAAACTGTAG ATCTCATTAA ATTCATAATG TCATTAAAAT CCTTCTCAGA TGCCACAGCG
    CCAACACAAG AGAAAATATT TGCTGTTTTA TGCATGCGTT GCCAGTCCAT TTTGAACATG
    GCGATGTTTC GTTGTAAAAA AGACATAGCA ATAAAGTATT CTCGTACTCT TAATAAACAC
    TTCGAGAGTT CTTCCAAAGT CGCCCAGGCA CCTTCTCCAT GCATTGCAAG CACAGGCACA
    CCATCCCCTC TTTCCCCAAT GCCTTCTCCT GCCAGCTCCG TAGGGTCCCA GTCAAGTGCT
    GGCAGTGTGG GGAGCAGTGG GGTGGCTGCC ACTATCAGCA CCCCAGTCAC CATCCAGAAT
    ATGACATCTT CCTATGTCAC CATCACATCC CATGTTCTTA CCGCCTTTGA CCTTTGGGAA
    CAGGCCGAGG CCCTCACGAG GAAGAATAAA GAATTCTTTG CTCGGCTCAG CACAAATGTG
    TGCACCTTGG CCCTCAACAG CAGTTTGGTG GACCTGGTGC ACTATACACG ACAGGGTTTT
    CAGCAGCTAC AAGAATTAAC CAAAACACCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005926.1
    CDS408..4040
    Translation

    Target ORF information:

    RefSeq Version NM_005935.3
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005935.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAGCCC AGTCAAGTTT GTACAATGAC GACAGAAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC CCACCAAGAG AAAGAGGCAT TTCCTGAAAA GATTCCCCTT
    TTTGGAGAGC CCTACAAGAC AGCAAAAGGT GATGAGCTGT CTAGTCGAAT ACAGAACATG
    TTGGGAAACT ACGAAGAAGT GAAGGAGTTC CTTAGTACTA AGTCTCACAC TCATCGCCTG
    GATGCTTCTG AAAATAGGTT GGGAAAGCCG AAATATCCTT TAATTCCTGA CAAAGGGAGC
    AGCATTCCAT CCAGCTCCTT CCACACTAGT GTCCACCACC AGTCCATTCA CACTCCTGCG
    TCTGGACCAC TTTCTGTTGG CAACATTAGC CACAATCCAA AGATGGCGCA GCCAAGAACT
    GAACCAATGC CAAGTCTCCA TGCCAAAAGC TGCGGCCCAC CGGACAGCCA GCACCTGACC
    CAGGATCGCC TTGGTCAGGA GGGGTTCGGC TCTAGTCATC ACAAGAAAGG TGACCGAAGA
    GCTGACGGAG ACCACTGTGC TTCGGTGACA GATTCGGCTC CAGAGAGGGA GCTTTCTCCC
    TTAATCTCTT TGCCTTCCCC AGTTCCCCCT TTGTCACCTA TACATTCCAA CCAGCAAACT
    CTTCCCCGGA CGCAAGGAAG CAGCAAGGTT CATGGCAGCA GCAATAACAG TAAAGGCTAT
    TGCCCAGCCA AATCTCCCAA GGACCTAGCA GTGAAAGTCC ATGATAAAGA GACCCCTCAA
    GACAGTTTGG TGGCCCCTGC CCAGCCGCCT TCTCAGACAT TTCCACCTCC CTCCCTCCCC
    TCAAAAAGTG TTGCAATGCA GCAGAAGCCC ACGGCTTATG TCCGGCCCAT GGATGGTCAA
    GATCAGGCCC CTAGTGAATC CCCTGAACTG AAACCACTGC CGGAGGACTA TCGACAGCAG
    ACCTTTGAAA AAACAGACTT GAAAGTGCCT GCCAAAGCCA AGCTCACCAA ACTGAAGATG
    CCTTCTCAGT CAGTTGAGCA GACCTACTCC AATGAAGTCC ATTGTGTTGA AGAGATTCTG
    AAGGAAATGA CCCATTCATG GCCGCCTCCT TTGACAGCAA TACATACGCC TAGTACAGCT
    GAGCCATCCA AGTTTCCTTT CCCTACAAAG GACTCTCAGC ATGTCAGTTC TGTAACCCAA
    AACCAAAAAC AATATGATAC ATCTTCAAAA ACTCACTCAA ATTCTCAGCA AGGAACGTCA
    TCCATGCTCG AAGACGACCT TCAGCTCAGT GACAGTGAGG ACAGTGACAG TGAACAAACC
    CCAGAGAAGC CTCCCTCCTC ATCTGCACCT CCAAGTGCTC CACAGTCCCT TCCAGAACCA
    GTGGCATCAG CACATTCCAG CAGTGCAGAG TCAGAAAGCA CCAGTGACTC AGACAGTTCC
    TCAGACTCAG AGAGCGAGAG CAGTTCAAGT GACAGCGAAG AAAATGAGCC CCTAGAAACC
    CCAGCTCCGG AGCCTGAGCC TCCAACAACA AACAAATGGC AGCTGGACAA CTGGCTGACC
    AAAGTCAGCC AGCCAGCTGC GCCACCAGAG GGCCCCAGGA GCACAGAGCC CCCACGGCGG
    CACCCAGAGA GTAAGGGCAG CAGCGACAGT GCCACGAGTC AGGAGCATTC TGAATCCAAA
    GATCCTCCCC CTAAAAGCTC CAGCAAAGCC CCCCGGGCCC CACCCGAAGC CCCCCACCCC
    GGAAAGAGGA GCTGTCAGAA GTCTCCGGCA CAGCAGGAGC CCCCACAAAG GCAAACCGTT
    GGAACCAAAC AACCCAAAAA ACCTGTCAAG GCCTCTGCCC GGGCAGGTTC ACGGACCAGC
    CTGCAGGGGG AAAGGGAGCC AGGGCTTCTT CCCTATGGCT CCCGAGACCA GACTTCCAAA
    GACAAGCCCA AGGTGAAGAC GAAAGGACGG CCCCGGGCCG CAGCAAGCAA CGAACCCAAG
    CCAGCAGTGC CCCCCTCCAG TGAGAAGAAG AAGCACAAGA GCTCCCTCCC TGCCCCCTCT
    AAGGCTCTCT CAGGCCCAGA ACCCGCGAAG GACAATGTGG AGGACAGGAC CCCTGAGCAC
    TTTGCTCTTG TTCCCCTGAC TGAGAGCCAG GGCCCACCCC ACAGTGGCAG CGGCAGCAGG
    ACTAGTGGCT GCCGCCAAGC CGTGGTGGTC CAGGAGGACA GCCGCAAAGA CAGACTCCCA
    TTGCCTTTGA GAGACACCAA GCTGCTCTCA CCGCTCAGGG ACACTCCTCC CCCACAAAGC
    TTGATGGTGA AGATCACCCT AGACCTGCTC TCTCGGATAC CCCAGCCTCC CGGGAAGGGG
    AGCCGCCAGA GGAAAGCAGA AGATAAACAG CCGCCCGCAG GGAAGAAGCA CAGCTCTGAG
    AAGAGGAGCT CAGACAGCTC AAGCAAGTTG GCCAAAAAGA GAAAGGGTGA AGCAGAAAGA
    GACTGTGATA ACAAGAAAAT CAGACTGGAG AAGGAAATCA AATCACAGTC ATCTTCATCT
    TCATCCTCCC ACAAAGAATC TTCTAAAACA AAGCCCTCCA GGCCCTCCTC ACAGTCCTCA
    AAGAAGGAAA TGCTCCCCCC GCCACCCGTG TCCTCGTCCT CCCAGAAGCC AGCCAAGCCT
    GCACTTAAGA GGTCAAGGCG GGAAGCAGAC ACCTGTGGCC AGGACCCTCC CAAAAGTGCC
    AGCAGTACCA AGAGCAACCA CAAAGACTCT TCCATTCCCA AGCAGAGAAG AGTAGAGGGG
    AAGGGCTCCA GAAGCTCCTC GGAGCACAAG GGTTCTTCCG GAGATACTGC AAATCCTTTT
    CCAGTGCCTT CTTTGCCAAA TGGTAACTCT AAACCAGGGA AGCCTCAAGT GAAGTTTGAC
    AAACAACAAG CAGACCTTCA CATGAGGGAG GCAAAAAAGA TGAAGCAGAA AGCAGAGTTA
    ATGACGGACA GGGTTGGAAA GGCTTTTAAG TACCTGGAAG CCGTCTTGTC CTTCATTGAG
    TGCGGAATTG CCACAGAGTC TGAAAGCCAG TCATCCAAGT CAGCTTACTC TGTCTACTCA
    GAAACTGTAG ATCTCATTAA ATTCATAATG TCATTAAAAT CCTTCTCAGA TGCCACAGCG
    CCAACACAAG AGAAAATATT TGCTGTTTTA TGCATGCGTT GCCAGTCCAT TTTGAACATG
    GCGATGTTTC GTTGTAAAAA AGACATAGCA ATAAAGTATT CTCGTACTCT TAATAAACAC
    TTCGAGAGTT CTTCCAAAGT CGCCCAGGCA CCTTCTCCAT GCATTGCAAG CACAGGCACA
    CCATCCCCTC TTTCCCCAAT GCCTTCTCCT GCCAGCTCCG TAGGGTCCCA GTCAAGTGCT
    GGCAGTGTGG GGAGCAGTGG GGTGGCTGCC ACTATCAGCA CCCCAGTCAC CATCCAGAAT
    ATGACATCTT CCTATGTCAC CATCACATCC CATGTTCTTA CCGCCTTTGA CCTTTGGGAA
    CAGGCCGAGG CCCTCACGAG GAAGAATAAA GAATTCTTTG CTCGGCTCAG CACAAATGTG
    TGCACCTTGG CCCTCAACAG CAGTTTGGTG GACCTGGTGC ACTATACACG ACAGGGTTTT
    CAGCAGCTAC AAGAATTAAC CAAAACACCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25298
    Clone ID Related Accession (Same CDS sequence) XM_011531973.3 , NM_001166693.2 , XM_005263007.3 , XM_011531973.2
    Accession Version NM_001166693.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-04
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC340279.1, AK300364.1, BC114930.1, L13773.1, AC092658.2 and AI638134.1. On Sep 16, 2015 this sequence version replaced NM_001166693.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300364.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCATTTA CAGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA
    TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG
    TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT
    AAGTCTCACA CTCATCGCCT GGATGCTTCT GAAAATAGGT TGGGAAAGCC GAAATATCCT
    TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC
    CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA
    AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGCGGCCCA
    CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT
    CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGATTCGGCT
    CCAGAGAGGG AGCTTTCTCC CTTAATCTCT TTGCCTTCCC CAGTTCCCCC TTTGTCACCT
    ATACATTCCA ACCAGCAAAC TCTTCCCCGG ACGCAAGGAA GCAGCAAGGT TCATGGCAGC
    AGCAATAACA GTAAAGGCTA TTGCCCAGCC AAATCTCCCA AGGACCTAGC AGTGAAAGTC
    CATGATAAAG AGACCCCTCA AGACAGTTTG GTGGCCCCTG CCCAGCCGCC TTCTCAGACA
    TTTCCACCTC CCTCCCTCCC CTCAAAAAGT GTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGATGGTCA AGATCAGGCC CCTAGTGAAT CCCCTGAACT GAAACCACTG
    CCGGAGGACT ATCGACAGCA GACCTTTGAA AAAACAGACT TGAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCTTCTCAG TCAGTTGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCATTCAT GGCCGCCTCC TTTGACAGCA
    ATACATACGC CTAGTACAGC TGAGCCATCC AAGTTTCCTT TCCCTACAAA GGACTCTCAG
    CATGTCAGTT CTGTAACCCA AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACTCA
    AATTCTCAGC AAGGAACGTC ATCCATGCTC GAAGACGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GTGAACAAAC CCCAGAGAAG CCTCCCTCCT CATCTGCACC TCCAAGTGCT
    CCACAGTCCC TTCCAGAACC AGTGGCATCA GCACATTCCA GCAGTGCAGA GTCAGAAAGC
    ACCAGTGACT CAGACAGTTC CTCAGACTCA GAGAGCGAGA GCAGTTCAAG TGACAGCGAA
    GAAAATGAGC CCCTAGAAAC CCCAGCTCCG GAGCCTGAGC CTCCAACAAC AAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCAGCTG CGCCACCAGA GGGCCCCAGG
    AGCACAGAGC CCCCACGGCG GCACCCAGAG AGTAAGGGCA GCAGCGACAG TGCCACGAGT
    CAGGAGCATT CTGAATCCAA AGATCCTCCC CCTAAAAGCT CCAGCAAAGC CCCCCGGGCC
    CCACCCGAAG CCCCCCACCC CGGAAAGAGG AGCTGTCAGA AGTCTCCGGC ACAGCAGGAG
    CCCCCACAAA GGCAAACCGT TGGAACCAAA CAACCCAAAA AACCTGTCAA GGCCTCTGCC
    CGGGCAGGTT CACGGACCAG CCTGCAGGGG GAAAGGGAGC CAGGGCTTCT TCCCTATGGC
    TCCCGAGACC AGACTTCCAA AGACAAGCCC AAGGTGAAGA CGAAAGGACG GCCCCGGGCC
    GCAGCAAGCA ACGAACCCAA GCCAGCAGTG CCCCCCTCCA GTGAGAAGAA GAAGCACAAG
    AGCTCCCTCC CTGCCCCCTC TAAGGCTCTC TCAGGCCCAG AACCCGCGAA GGACAATGTG
    GAGGACAGGA CCCCTGAGCA CTTTGCTCTT GTTCCCCTGA CTGAGAGCCA GGGCCCACCC
    CACAGTGGCA GCGGCAGCAG GACTAGTGGC TGCCGCCAAG CCGTGGTGGT CCAGGAGGAC
    AGCCGCAAAG ACAGACTCCC ATTGCCTTTG AGAGACACCA AGCTGCTCTC ACCGCTCAGG
    GACACTCCTC CCCCACAAAG CTTGATGGTG AAGATCACCC TAGACCTGCT CTCTCGGATA
    CCCCAGCCTC CCGGGAAGGG GAGCCGCCAG AGGAAAGCAG AAGATAAACA GCCGCCCGCA
    GGGAAGAAGC ACAGCTCTGA GAAGAGGAGC TCAGACAGCT CAAGCAAGTT GGCCAAAAAG
    AGAAAGGGTG AAGCAGAAAG AGACTGTGAT AACAAGAAAA TCAGACTGGA GAAGGAAATC
    AAATCACAGT CATCTTCATC TTCATCCTCC CACAAAGAAT CTTCTAAAAC AAAGCCCTCC
    AGGCCCTCCT CACAGTCCTC AAAGAAGGAA ATGCTCCCCC CGCCACCCGT GTCCTCGTCC
    TCCCAGAAGC CAGCCAAGCC TGCACTTAAG AGGTCAAGGC GGGAAGCAGA CACCTGTGGC
    CAGGACCCTC CCAAAAGTGC CAGCAGTACC AAGAGCAACC ACAAAGACTC TTCCATTCCC
    AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC AGAAGCTCCT CGGAGCACAA GGGTTCTTCC
    GGAGATACTG CAAATCCTTT TCCAGTGCCT TCTTTGCCAA ATGGTAACTC TAAACCAGGG
    AAGCCTCAAG TGAAGTTTGA CAAACAACAA GCAGACCTTC ACATGAGGGA GGCAAAAAAG
    ATGAAGCAGA AAGCAGAGTT AATGACGGAC AGGGTTGGAA AGGCTTTTAA GTACCTGGAA
    GCCGTCTTGT CCTTCATTGA GTGCGGAATT GCCACAGAGT CTGAAAGCCA GTCATCCAAG
    TCAGCTTACT CTGTCTACTC AGAAACTGTA GATCTCATTA AATTCATAAT GTCATTAAAA
    TCCTTCTCAG ATGCCACAGC GCCAACACAA GAGAAAATAT TTGCTGTTTT ATGCATGCGT
    TGCCAGTCCA TTTTGAACAT GGCGATGTTT CGTTGTAAAA AAGACATAGC AATAAAGTAT
    TCTCGTACTC TTAATAAACA CTTCGAGAGT TCTTCCAAAG TCGCCCAGGC ACCTTCTCCA
    TGCATTGCAA GAAGCACAGG CACACCATCC CCTCTTTCCC CAATGCCTTC TCCTGCCAGC
    TCCGTAGGGT CCCAGTCAAG TGCTGGCAGT GTGGGGAGCA GTGGGGTGGC TGCCACTATC
    AGCACCCCAG TCACCATCCA GAATATGACA TCTTCCTATG TCACCATCAC ATCCCATGTT
    CTTACCGCCT TTGACCTTTG GGAACAGGCC GAGGCCCTCA CGAGGAAGAA TAAAGAATTC
    TTTGCTCGGC TCAGCACAAA TGTGTGCACC TTGGCCCTCA ACAGCAGTTT GGTGGACCTG
    GTGCACTATA CACGACAGGG TTTTCAGCAG CTACAAGAAT TAACCAAAAC ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001160165.1
    CDS276..3932
    Translation

    Target ORF information:

    RefSeq Version NM_001166693.2
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001166693.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCATTTA CAGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA
    TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG
    TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT
    AAGTCTCACA CTCATCGCCT GGATGCTTCT GAAAATAGGT TGGGAAAGCC GAAATATCCT
    TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC
    CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA
    AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGCGGCCCA
    CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT
    CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGATTCGGCT
    CCAGAGAGGG AGCTTTCTCC CTTAATCTCT TTGCCTTCCC CAGTTCCCCC TTTGTCACCT
    ATACATTCCA ACCAGCAAAC TCTTCCCCGG ACGCAAGGAA GCAGCAAGGT TCATGGCAGC
    AGCAATAACA GTAAAGGCTA TTGCCCAGCC AAATCTCCCA AGGACCTAGC AGTGAAAGTC
    CATGATAAAG AGACCCCTCA AGACAGTTTG GTGGCCCCTG CCCAGCCGCC TTCTCAGACA
    TTTCCACCTC CCTCCCTCCC CTCAAAAAGT GTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGATGGTCA AGATCAGGCC CCTAGTGAAT CCCCTGAACT GAAACCACTG
    CCGGAGGACT ATCGACAGCA GACCTTTGAA AAAACAGACT TGAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCTTCTCAG TCAGTTGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCATTCAT GGCCGCCTCC TTTGACAGCA
    ATACATACGC CTAGTACAGC TGAGCCATCC AAGTTTCCTT TCCCTACAAA GGACTCTCAG
    CATGTCAGTT CTGTAACCCA AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACTCA
    AATTCTCAGC AAGGAACGTC ATCCATGCTC GAAGACGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GTGAACAAAC CCCAGAGAAG CCTCCCTCCT CATCTGCACC TCCAAGTGCT
    CCACAGTCCC TTCCAGAACC AGTGGCATCA GCACATTCCA GCAGTGCAGA GTCAGAAAGC
    ACCAGTGACT CAGACAGTTC CTCAGACTCA GAGAGCGAGA GCAGTTCAAG TGACAGCGAA
    GAAAATGAGC CCCTAGAAAC CCCAGCTCCG GAGCCTGAGC CTCCAACAAC AAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCAGCTG CGCCACCAGA GGGCCCCAGG
    AGCACAGAGC CCCCACGGCG GCACCCAGAG AGTAAGGGCA GCAGCGACAG TGCCACGAGT
    CAGGAGCATT CTGAATCCAA AGATCCTCCC CCTAAAAGCT CCAGCAAAGC CCCCCGGGCC
    CCACCCGAAG CCCCCCACCC CGGAAAGAGG AGCTGTCAGA AGTCTCCGGC ACAGCAGGAG
    CCCCCACAAA GGCAAACCGT TGGAACCAAA CAACCCAAAA AACCTGTCAA GGCCTCTGCC
    CGGGCAGGTT CACGGACCAG CCTGCAGGGG GAAAGGGAGC CAGGGCTTCT TCCCTATGGC
    TCCCGAGACC AGACTTCCAA AGACAAGCCC AAGGTGAAGA CGAAAGGACG GCCCCGGGCC
    GCAGCAAGCA ACGAACCCAA GCCAGCAGTG CCCCCCTCCA GTGAGAAGAA GAAGCACAAG
    AGCTCCCTCC CTGCCCCCTC TAAGGCTCTC TCAGGCCCAG AACCCGCGAA GGACAATGTG
    GAGGACAGGA CCCCTGAGCA CTTTGCTCTT GTTCCCCTGA CTGAGAGCCA GGGCCCACCC
    CACAGTGGCA GCGGCAGCAG GACTAGTGGC TGCCGCCAAG CCGTGGTGGT CCAGGAGGAC
    AGCCGCAAAG ACAGACTCCC ATTGCCTTTG AGAGACACCA AGCTGCTCTC ACCGCTCAGG
    GACACTCCTC CCCCACAAAG CTTGATGGTG AAGATCACCC TAGACCTGCT CTCTCGGATA
    CCCCAGCCTC CCGGGAAGGG GAGCCGCCAG AGGAAAGCAG AAGATAAACA GCCGCCCGCA
    GGGAAGAAGC ACAGCTCTGA GAAGAGGAGC TCAGACAGCT CAAGCAAGTT GGCCAAAAAG
    AGAAAGGGTG AAGCAGAAAG AGACTGTGAT AACAAGAAAA TCAGACTGGA GAAGGAAATC
    AAATCACAGT CATCTTCATC TTCATCCTCC CACAAAGAAT CTTCTAAAAC AAAGCCCTCC
    AGGCCCTCCT CACAGTCCTC AAAGAAGGAA ATGCTCCCCC CGCCACCCGT GTCCTCGTCC
    TCCCAGAAGC CAGCCAAGCC TGCACTTAAG AGGTCAAGGC GGGAAGCAGA CACCTGTGGC
    CAGGACCCTC CCAAAAGTGC CAGCAGTACC AAGAGCAACC ACAAAGACTC TTCCATTCCC
    AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC AGAAGCTCCT CGGAGCACAA GGGTTCTTCC
    GGAGATACTG CAAATCCTTT TCCAGTGCCT TCTTTGCCAA ATGGTAACTC TAAACCAGGG
    AAGCCTCAAG TGAAGTTTGA CAAACAACAA GCAGACCTTC ACATGAGGGA GGCAAAAAAG
    ATGAAGCAGA AAGCAGAGTT AATGACGGAC AGGGTTGGAA AGGCTTTTAA GTACCTGGAA
    GCCGTCTTGT CCTTCATTGA GTGCGGAATT GCCACAGAGT CTGAAAGCCA GTCATCCAAG
    TCAGCTTACT CTGTCTACTC AGAAACTGTA GATCTCATTA AATTCATAAT GTCATTAAAA
    TCCTTCTCAG ATGCCACAGC GCCAACACAA GAGAAAATAT TTGCTGTTTT ATGCATGCGT
    TGCCAGTCCA TTTTGAACAT GGCGATGTTT CGTTGTAAAA AAGACATAGC AATAAAGTAT
    TCTCGTACTC TTAATAAACA CTTCGAGAGT TCTTCCAAAG TCGCCCAGGC ACCTTCTCCA
    TGCATTGCAA GAAGCACAGG CACACCATCC CCTCTTTCCC CAATGCCTTC TCCTGCCAGC
    TCCGTAGGGT CCCAGTCAAG TGCTGGCAGT GTGGGGAGCA GTGGGGTGGC TGCCACTATC
    AGCACCCCAG TCACCATCCA GAATATGACA TCTTCCTATG TCACCATCAC ATCCCATGTT
    CTTACCGCCT TTGACCTTTG GGAACAGGCC GAGGCCCTCA CGAGGAAGAA TAAAGAATTC
    TTTGCTCGGC TCAGCACAAA TGTGTGCACC TTGGCCCTCA ACAGCAGTTT GGTGGACCTG
    GTGCACTATA CACGACAGGG TTTTCAGCAG CTACAAGAAT TAACCAAAAC ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38943
    Clone ID Related Accession (Same CDS sequence) NM_001313959.1
    Accession Version NM_001313959.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L13773.1, BC114930.1, AC092658.2 and AI638134.1. On Sep 16, 2015 this sequence version replaced XM_005263012.1. Transcript Variant: This variant (3) contains a distinct 5' UTR and uses an alternate translation start site compared to variant 1. It encodes isoform 2, which has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC114930.1, L25050.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAGCCC AGTCAAGTTT GTACAATGAC GACAGAAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC CCACCAAGAG AAAGAGGCAT TTCCTGAAAA GATTCCCCTT
    TTTGGAGAGC CCTACAAGAC AGCAAAAGGT GATGAGCTGT CTAGTCGAAT ACAGAACATG
    TTGGGAAACT ACGAAGAAGT GAAGGAGTTC CTTAGTACTA AGTCTCACAC TCATCGCCTG
    GATGCTTCTG AAAATAGGTT GGGAAAGCCG AAATATCCTT TAATTCCTGA CAAAGGGAGC
    AGCATTCCAT CCAGCTCCTT CCACACTAGT GTCCACCACC AGTCCATTCA CACTCCTGCG
    TCTGGACCAC TTTCTGTTGG CAACATTAGC CACAATCCAA AGATGGCGCA GCCAAGAACT
    GAACCAATGC CAAGTCTCCA TGCCAAAAGC TGCGGCCCAC CGGACAGCCA GCACCTGACC
    CAGGATCGCC TTGGTCAGGA GGGGTTCGGC TCTAGTCATC ACAAGAAAGG TGACCGAAGA
    GCTGACGGAG ACCACTGTGC TTCGGTGACA GATTCGGCTC CAGAGAGGGA GCTTTCTCCC
    TTAATCTCTT TGCCTTCCCC AGTTCCCCCT TTGTCACCTA TACATTCCAA CCAGCAAACT
    CTTCCCCGGA CGCAAGGAAG CAGCAAGGTT CATGGCAGCA GCAATAACAG TAAAGGCTAT
    TGCCCAGCCA AATCTCCCAA GGACCTAGCA GTGAAAGTCC ATGATAAAGA GACCCCTCAA
    GACAGTTTGG TGGCCCCTGC CCAGCCGCCT TCTCAGACAT TTCCACCTCC CTCCCTCCCC
    TCAAAAAGTG TTGCAATGCA GCAGAAGCCC ACGGCTTATG TCCGGCCCAT GGATGGTCAA
    GATCAGGCCC CTAGTGAATC CCCTGAACTG AAACCACTGC CGGAGGACTA TCGACAGCAG
    ACCTTTGAAA AAACAGACTT GAAAGTGCCT GCCAAAGCCA AGCTCACCAA ACTGAAGATG
    CCTTCTCAGT CAGTTGAGCA GACCTACTCC AATGAAGTCC ATTGTGTTGA AGAGATTCTG
    AAGGAAATGA CCCATTCATG GCCGCCTCCT TTGACAGCAA TACATACGCC TAGTACAGCT
    GAGCCATCCA AGTTTCCTTT CCCTACAAAG GACTCTCAGC ATGTCAGTTC TGTAACCCAA
    AACCAAAAAC AATATGATAC ATCTTCAAAA ACTCACTCAA ATTCTCAGCA AGGAACGTCA
    TCCATGCTCG AAGACGACCT TCAGCTCAGT GACAGTGAGG ACAGTGACAG TGAACAAACC
    CCAGAGAAGC CTCCCTCCTC ATCTGCACCT CCAAGTGCTC CACAGTCCCT TCCAGAACCA
    GTGGCATCAG CACATTCCAG CAGTGCAGAG TCAGAAAGCA CCAGTGACTC AGACAGTTCC
    TCAGACTCAG AGAGCGAGAG CAGTTCAAGT GACAGCGAAG AAAATGAGCC CCTAGAAACC
    CCAGCTCCGG AGCCTGAGCC TCCAACAACA AACAAATGGC AGCTGGACAA CTGGCTGACC
    AAAGTCAGCC AGCCAGCTGC GCCACCAGAG GGCCCCAGGA GCACAGAGCC CCCACGGCGG
    CACCCAGAGA GTAAGGGCAG CAGCGACAGT GCCACGAGTC AGGAGCATTC TGAATCCAAA
    GATCCTCCCC CTAAAAGCTC CAGCAAAGCC CCCCGGGCCC CACCCGAAGC CCCCCACCCC
    GGAAAGAGGA GCTGTCAGAA GTCTCCGGCA CAGCAGGAGC CCCCACAAAG GCAAACCGTT
    GGAACCAAAC AACCCAAAAA ACCTGTCAAG GCCTCTGCCC GGGCAGGTTC ACGGACCAGC
    CTGCAGGGGG AAAGGGAGCC AGGGCTTCTT CCCTATGGCT CCCGAGACCA GACTTCCAAA
    GACAAGCCCA AGGTGAAGAC GAAAGGACGG CCCCGGGCCG CAGCAAGCAA CGAACCCAAG
    CCAGCAGTGC CCCCCTCCAG TGAGAAGAAG AAGCACAAGA GCTCCCTCCC TGCCCCCTCT
    AAGGCTCTCT CAGGCCCAGA ACCCGCGAAG GACAATGTGG AGGACAGGAC CCCTGAGCAC
    TTTGCTCTTG TTCCCCTGAC TGAGAGCCAG GGCCCACCCC ACAGTGGCAG CGGCAGCAGG
    ACTAGTGGCT GCCGCCAAGC CGTGGTGGTC CAGGAGGACA GCCGCAAAGA CAGACTCCCA
    TTGCCTTTGA GAGACACCAA GCTGCTCTCA CCGCTCAGGG ACACTCCTCC CCCACAAAGC
    TTGATGGTGA AGATCACCCT AGACCTGCTC TCTCGGATAC CCCAGCCTCC CGGGAAGGGG
    AGCCGCCAGA GGAAAGCAGA AGATAAACAG CCGCCCGCAG GGAAGAAGCA CAGCTCTGAG
    AAGAGGAGCT CAGACAGCTC AAGCAAGTTG GCCAAAAAGA GAAAGGGTGA AGCAGAAAGA
    GACTGTGATA ACAAGAAAAT CAGACTGGAG AAGGAAATCA AATCACAGTC ATCTTCATCT
    TCATCCTCCC ACAAAGAATC TTCTAAAACA AAGCCCTCCA GGCCCTCCTC ACAGTCCTCA
    AAGAAGGAAA TGCTCCCCCC GCCACCCGTG TCCTCGTCCT CCCAGAAGCC AGCCAAGCCT
    GCACTTAAGA GGTCAAGGCG GGAAGCAGAC ACCTGTGGCC AGGACCCTCC CAAAAGTGCC
    AGCAGTACCA AGAGCAACCA CAAAGACTCT TCCATTCCCA AGCAGAGAAG AGTAGAGGGG
    AAGGGCTCCA GAAGCTCCTC GGAGCACAAG GGTTCTTCCG GAGATACTGC AAATCCTTTT
    CCAGTGCCTT CTTTGCCAAA TGGTAACTCT AAACCAGGGA AGCCTCAAGT GAAGTTTGAC
    AAACAACAAG CAGACCTTCA CATGAGGGAG GCAAAAAAGA TGAAGCAGAA AGCAGAGTTA
    ATGACGGACA GGGTTGGAAA GGCTTTTAAG TACCTGGAAG CCGTCTTGTC CTTCATTGAG
    TGCGGAATTG CCACAGAGTC TGAAAGCCAG TCATCCAAGT CAGCTTACTC TGTCTACTCA
    GAAACTGTAG ATCTCATTAA ATTCATAATG TCATTAAAAT CCTTCTCAGA TGCCACAGCG
    CCAACACAAG AGAAAATATT TGCTGTTTTA TGCATGCGTT GCCAGTCCAT TTTGAACATG
    GCGATGTTTC GTTGTAAAAA AGACATAGCA ATAAAGTATT CTCGTACTCT TAATAAACAC
    TTCGAGAGTT CTTCCAAAGT CGCCCAGGCA CCTTCTCCAT GCATTGCAAG AAGCACAGGC
    ACACCATCCC CTCTTTCCCC AATGCCTTCT CCTGCCAGCT CCGTAGGGTC CCAGTCAAGT
    GCTGGCAGTG TGGGGAGCAG TGGGGTGGCT GCCACTATCA GCACCCCAGT CACCATCCAG
    AATATGACAT CTTCCTATGT CACCATCACA TCCCATGTTC TTACCGCCTT TGACCTTTGG
    GAACAGGCCG AGGCCCTCAC GAGGAAGAAT AAAGAATTCT TTGCTCGGCT CAGCACAAAT
    GTGTGCACCT TGGCCCTCAA CAGCAGTTTG GTGGACCTGG TGCACTATAC ACGACAGGGT
    TTTCAGCAGC TACAAGAATT AACCAAAACA CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001300888.1
    CDS421..4056
    Translation

    Target ORF information:

    RefSeq Version NM_001313959.1
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001313959.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAGCCC AGTCAAGTTT GTACAATGAC GACAGAAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC CCACCAAGAG AAAGAGGCAT TTCCTGAAAA GATTCCCCTT
    TTTGGAGAGC CCTACAAGAC AGCAAAAGGT GATGAGCTGT CTAGTCGAAT ACAGAACATG
    TTGGGAAACT ACGAAGAAGT GAAGGAGTTC CTTAGTACTA AGTCTCACAC TCATCGCCTG
    GATGCTTCTG AAAATAGGTT GGGAAAGCCG AAATATCCTT TAATTCCTGA CAAAGGGAGC
    AGCATTCCAT CCAGCTCCTT CCACACTAGT GTCCACCACC AGTCCATTCA CACTCCTGCG
    TCTGGACCAC TTTCTGTTGG CAACATTAGC CACAATCCAA AGATGGCGCA GCCAAGAACT
    GAACCAATGC CAAGTCTCCA TGCCAAAAGC TGCGGCCCAC CGGACAGCCA GCACCTGACC
    CAGGATCGCC TTGGTCAGGA GGGGTTCGGC TCTAGTCATC ACAAGAAAGG TGACCGAAGA
    GCTGACGGAG ACCACTGTGC TTCGGTGACA GATTCGGCTC CAGAGAGGGA GCTTTCTCCC
    TTAATCTCTT TGCCTTCCCC AGTTCCCCCT TTGTCACCTA TACATTCCAA CCAGCAAACT
    CTTCCCCGGA CGCAAGGAAG CAGCAAGGTT CATGGCAGCA GCAATAACAG TAAAGGCTAT
    TGCCCAGCCA AATCTCCCAA GGACCTAGCA GTGAAAGTCC ATGATAAAGA GACCCCTCAA
    GACAGTTTGG TGGCCCCTGC CCAGCCGCCT TCTCAGACAT TTCCACCTCC CTCCCTCCCC
    TCAAAAAGTG TTGCAATGCA GCAGAAGCCC ACGGCTTATG TCCGGCCCAT GGATGGTCAA
    GATCAGGCCC CTAGTGAATC CCCTGAACTG AAACCACTGC CGGAGGACTA TCGACAGCAG
    ACCTTTGAAA AAACAGACTT GAAAGTGCCT GCCAAAGCCA AGCTCACCAA ACTGAAGATG
    CCTTCTCAGT CAGTTGAGCA GACCTACTCC AATGAAGTCC ATTGTGTTGA AGAGATTCTG
    AAGGAAATGA CCCATTCATG GCCGCCTCCT TTGACAGCAA TACATACGCC TAGTACAGCT
    GAGCCATCCA AGTTTCCTTT CCCTACAAAG GACTCTCAGC ATGTCAGTTC TGTAACCCAA
    AACCAAAAAC AATATGATAC ATCTTCAAAA ACTCACTCAA ATTCTCAGCA AGGAACGTCA
    TCCATGCTCG AAGACGACCT TCAGCTCAGT GACAGTGAGG ACAGTGACAG TGAACAAACC
    CCAGAGAAGC CTCCCTCCTC ATCTGCACCT CCAAGTGCTC CACAGTCCCT TCCAGAACCA
    GTGGCATCAG CACATTCCAG CAGTGCAGAG TCAGAAAGCA CCAGTGACTC AGACAGTTCC
    TCAGACTCAG AGAGCGAGAG CAGTTCAAGT GACAGCGAAG AAAATGAGCC CCTAGAAACC
    CCAGCTCCGG AGCCTGAGCC TCCAACAACA AACAAATGGC AGCTGGACAA CTGGCTGACC
    AAAGTCAGCC AGCCAGCTGC GCCACCAGAG GGCCCCAGGA GCACAGAGCC CCCACGGCGG
    CACCCAGAGA GTAAGGGCAG CAGCGACAGT GCCACGAGTC AGGAGCATTC TGAATCCAAA
    GATCCTCCCC CTAAAAGCTC CAGCAAAGCC CCCCGGGCCC CACCCGAAGC CCCCCACCCC
    GGAAAGAGGA GCTGTCAGAA GTCTCCGGCA CAGCAGGAGC CCCCACAAAG GCAAACCGTT
    GGAACCAAAC AACCCAAAAA ACCTGTCAAG GCCTCTGCCC GGGCAGGTTC ACGGACCAGC
    CTGCAGGGGG AAAGGGAGCC AGGGCTTCTT CCCTATGGCT CCCGAGACCA GACTTCCAAA
    GACAAGCCCA AGGTGAAGAC GAAAGGACGG CCCCGGGCCG CAGCAAGCAA CGAACCCAAG
    CCAGCAGTGC CCCCCTCCAG TGAGAAGAAG AAGCACAAGA GCTCCCTCCC TGCCCCCTCT
    AAGGCTCTCT CAGGCCCAGA ACCCGCGAAG GACAATGTGG AGGACAGGAC CCCTGAGCAC
    TTTGCTCTTG TTCCCCTGAC TGAGAGCCAG GGCCCACCCC ACAGTGGCAG CGGCAGCAGG
    ACTAGTGGCT GCCGCCAAGC CGTGGTGGTC CAGGAGGACA GCCGCAAAGA CAGACTCCCA
    TTGCCTTTGA GAGACACCAA GCTGCTCTCA CCGCTCAGGG ACACTCCTCC CCCACAAAGC
    TTGATGGTGA AGATCACCCT AGACCTGCTC TCTCGGATAC CCCAGCCTCC CGGGAAGGGG
    AGCCGCCAGA GGAAAGCAGA AGATAAACAG CCGCCCGCAG GGAAGAAGCA CAGCTCTGAG
    AAGAGGAGCT CAGACAGCTC AAGCAAGTTG GCCAAAAAGA GAAAGGGTGA AGCAGAAAGA
    GACTGTGATA ACAAGAAAAT CAGACTGGAG AAGGAAATCA AATCACAGTC ATCTTCATCT
    TCATCCTCCC ACAAAGAATC TTCTAAAACA AAGCCCTCCA GGCCCTCCTC ACAGTCCTCA
    AAGAAGGAAA TGCTCCCCCC GCCACCCGTG TCCTCGTCCT CCCAGAAGCC AGCCAAGCCT
    GCACTTAAGA GGTCAAGGCG GGAAGCAGAC ACCTGTGGCC AGGACCCTCC CAAAAGTGCC
    AGCAGTACCA AGAGCAACCA CAAAGACTCT TCCATTCCCA AGCAGAGAAG AGTAGAGGGG
    AAGGGCTCCA GAAGCTCCTC GGAGCACAAG GGTTCTTCCG GAGATACTGC AAATCCTTTT
    CCAGTGCCTT CTTTGCCAAA TGGTAACTCT AAACCAGGGA AGCCTCAAGT GAAGTTTGAC
    AAACAACAAG CAGACCTTCA CATGAGGGAG GCAAAAAAGA TGAAGCAGAA AGCAGAGTTA
    ATGACGGACA GGGTTGGAAA GGCTTTTAAG TACCTGGAAG CCGTCTTGTC CTTCATTGAG
    TGCGGAATTG CCACAGAGTC TGAAAGCCAG TCATCCAAGT CAGCTTACTC TGTCTACTCA
    GAAACTGTAG ATCTCATTAA ATTCATAATG TCATTAAAAT CCTTCTCAGA TGCCACAGCG
    CCAACACAAG AGAAAATATT TGCTGTTTTA TGCATGCGTT GCCAGTCCAT TTTGAACATG
    GCGATGTTTC GTTGTAAAAA AGACATAGCA ATAAAGTATT CTCGTACTCT TAATAAACAC
    TTCGAGAGTT CTTCCAAAGT CGCCCAGGCA CCTTCTCCAT GCATTGCAAG AAGCACAGGC
    ACACCATCCC CTCTTTCCCC AATGCCTTCT CCTGCCAGCT CCGTAGGGTC CCAGTCAAGT
    GCTGGCAGTG TGGGGAGCAG TGGGGTGGCT GCCACTATCA GCACCCCAGT CACCATCCAG
    AATATGACAT CTTCCTATGT CACCATCACA TCCCATGTTC TTACCGCCTT TGACCTTTGG
    GAACAGGCCG AGGCCCTCAC GAGGAAGAAT AAAGAATTCT TTGCTCGGCT CAGCACAAAT
    GTGTGCACCT TGGCCCTCAA CAGCAGTTTG GTGGACCTGG TGCACTATAC ACGACAGGGT
    TTTCAGCAGC TACAAGAATT AACCAAAACA CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25298
    Clone ID Related Accession (Same CDS sequence) XM_011531973.3 , NM_001166693.2 , XM_005263007.3 , XM_011531973.2
    Accession Version XM_011531973.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011531973.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCATTTA CAGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA
    TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG
    TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT
    AAGTCTCACA CTCATCGCCT GGATGCTTCT GAAAATAGGT TGGGAAAGCC GAAATATCCT
    TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC
    CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA
    AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGCGGCCCA
    CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT
    CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGATTCGGCT
    CCAGAGAGGG AGCTTTCTCC CTTAATCTCT TTGCCTTCCC CAGTTCCCCC TTTGTCACCT
    ATACATTCCA ACCAGCAAAC TCTTCCCCGG ACGCAAGGAA GCAGCAAGGT TCATGGCAGC
    AGCAATAACA GTAAAGGCTA TTGCCCAGCC AAATCTCCCA AGGACCTAGC AGTGAAAGTC
    CATGATAAAG AGACCCCTCA AGACAGTTTG GTGGCCCCTG CCCAGCCGCC TTCTCAGACA
    TTTCCACCTC CCTCCCTCCC CTCAAAAAGT GTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGATGGTCA AGATCAGGCC CCTAGTGAAT CCCCTGAACT GAAACCACTG
    CCGGAGGACT ATCGACAGCA GACCTTTGAA AAAACAGACT TGAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCTTCTCAG TCAGTTGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCATTCAT GGCCGCCTCC TTTGACAGCA
    ATACATACGC CTAGTACAGC TGAGCCATCC AAGTTTCCTT TCCCTACAAA GGACTCTCAG
    CATGTCAGTT CTGTAACCCA AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACTCA
    AATTCTCAGC AAGGAACGTC ATCCATGCTC GAAGACGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GTGAACAAAC CCCAGAGAAG CCTCCCTCCT CATCTGCACC TCCAAGTGCT
    CCACAGTCCC TTCCAGAACC AGTGGCATCA GCACATTCCA GCAGTGCAGA GTCAGAAAGC
    ACCAGTGACT CAGACAGTTC CTCAGACTCA GAGAGCGAGA GCAGTTCAAG TGACAGCGAA
    GAAAATGAGC CCCTAGAAAC CCCAGCTCCG GAGCCTGAGC CTCCAACAAC AAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCAGCTG CGCCACCAGA GGGCCCCAGG
    AGCACAGAGC CCCCACGGCG GCACCCAGAG AGTAAGGGCA GCAGCGACAG TGCCACGAGT
    CAGGAGCATT CTGAATCCAA AGATCCTCCC CCTAAAAGCT CCAGCAAAGC CCCCCGGGCC
    CCACCCGAAG CCCCCCACCC CGGAAAGAGG AGCTGTCAGA AGTCTCCGGC ACAGCAGGAG
    CCCCCACAAA GGCAAACCGT TGGAACCAAA CAACCCAAAA AACCTGTCAA GGCCTCTGCC
    CGGGCAGGTT CACGGACCAG CCTGCAGGGG GAAAGGGAGC CAGGGCTTCT TCCCTATGGC
    TCCCGAGACC AGACTTCCAA AGACAAGCCC AAGGTGAAGA CGAAAGGACG GCCCCGGGCC
    GCAGCAAGCA ACGAACCCAA GCCAGCAGTG CCCCCCTCCA GTGAGAAGAA GAAGCACAAG
    AGCTCCCTCC CTGCCCCCTC TAAGGCTCTC TCAGGCCCAG AACCCGCGAA GGACAATGTG
    GAGGACAGGA CCCCTGAGCA CTTTGCTCTT GTTCCCCTGA CTGAGAGCCA GGGCCCACCC
    CACAGTGGCA GCGGCAGCAG GACTAGTGGC TGCCGCCAAG CCGTGGTGGT CCAGGAGGAC
    AGCCGCAAAG ACAGACTCCC ATTGCCTTTG AGAGACACCA AGCTGCTCTC ACCGCTCAGG
    GACACTCCTC CCCCACAAAG CTTGATGGTG AAGATCACCC TAGACCTGCT CTCTCGGATA
    CCCCAGCCTC CCGGGAAGGG GAGCCGCCAG AGGAAAGCAG AAGATAAACA GCCGCCCGCA
    GGGAAGAAGC ACAGCTCTGA GAAGAGGAGC TCAGACAGCT CAAGCAAGTT GGCCAAAAAG
    AGAAAGGGTG AAGCAGAAAG AGACTGTGAT AACAAGAAAA TCAGACTGGA GAAGGAAATC
    AAATCACAGT CATCTTCATC TTCATCCTCC CACAAAGAAT CTTCTAAAAC AAAGCCCTCC
    AGGCCCTCCT CACAGTCCTC AAAGAAGGAA ATGCTCCCCC CGCCACCCGT GTCCTCGTCC
    TCCCAGAAGC CAGCCAAGCC TGCACTTAAG AGGTCAAGGC GGGAAGCAGA CACCTGTGGC
    CAGGACCCTC CCAAAAGTGC CAGCAGTACC AAGAGCAACC ACAAAGACTC TTCCATTCCC
    AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC AGAAGCTCCT CGGAGCACAA GGGTTCTTCC
    GGAGATACTG CAAATCCTTT TCCAGTGCCT TCTTTGCCAA ATGGTAACTC TAAACCAGGG
    AAGCCTCAAG TGAAGTTTGA CAAACAACAA GCAGACCTTC ACATGAGGGA GGCAAAAAAG
    ATGAAGCAGA AAGCAGAGTT AATGACGGAC AGGGTTGGAA AGGCTTTTAA GTACCTGGAA
    GCCGTCTTGT CCTTCATTGA GTGCGGAATT GCCACAGAGT CTGAAAGCCA GTCATCCAAG
    TCAGCTTACT CTGTCTACTC AGAAACTGTA GATCTCATTA AATTCATAAT GTCATTAAAA
    TCCTTCTCAG ATGCCACAGC GCCAACACAA GAGAAAATAT TTGCTGTTTT ATGCATGCGT
    TGCCAGTCCA TTTTGAACAT GGCGATGTTT CGTTGTAAAA AAGACATAGC AATAAAGTAT
    TCTCGTACTC TTAATAAACA CTTCGAGAGT TCTTCCAAAG TCGCCCAGGC ACCTTCTCCA
    TGCATTGCAA GAAGCACAGG CACACCATCC CCTCTTTCCC CAATGCCTTC TCCTGCCAGC
    TCCGTAGGGT CCCAGTCAAG TGCTGGCAGT GTGGGGAGCA GTGGGGTGGC TGCCACTATC
    AGCACCCCAG TCACCATCCA GAATATGACA TCTTCCTATG TCACCATCAC ATCCCATGTT
    CTTACCGCCT TTGACCTTTG GGAACAGGCC GAGGCCCTCA CGAGGAAGAA TAAAGAATTC
    TTTGCTCGGC TCAGCACAAA TGTGTGCACC TTGGCCCTCA ACAGCAGTTT GGTGGACCTG
    GTGCACTATA CACGACAGGG TTTTCAGCAG CTACAAGAAT TAACCAAAAC ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011530275.1
    CDS154..3810
    Translation

    Target ORF information:

    RefSeq Version XM_011531973.3
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011531973.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCATTTA CAGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA
    TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG
    TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT
    AAGTCTCACA CTCATCGCCT GGATGCTTCT GAAAATAGGT TGGGAAAGCC GAAATATCCT
    TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC
    CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA
    AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGCGGCCCA
    CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT
    CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGATTCGGCT
    CCAGAGAGGG AGCTTTCTCC CTTAATCTCT TTGCCTTCCC CAGTTCCCCC TTTGTCACCT
    ATACATTCCA ACCAGCAAAC TCTTCCCCGG ACGCAAGGAA GCAGCAAGGT TCATGGCAGC
    AGCAATAACA GTAAAGGCTA TTGCCCAGCC AAATCTCCCA AGGACCTAGC AGTGAAAGTC
    CATGATAAAG AGACCCCTCA AGACAGTTTG GTGGCCCCTG CCCAGCCGCC TTCTCAGACA
    TTTCCACCTC CCTCCCTCCC CTCAAAAAGT GTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGATGGTCA AGATCAGGCC CCTAGTGAAT CCCCTGAACT GAAACCACTG
    CCGGAGGACT ATCGACAGCA GACCTTTGAA AAAACAGACT TGAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCTTCTCAG TCAGTTGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCATTCAT GGCCGCCTCC TTTGACAGCA
    ATACATACGC CTAGTACAGC TGAGCCATCC AAGTTTCCTT TCCCTACAAA GGACTCTCAG
    CATGTCAGTT CTGTAACCCA AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACTCA
    AATTCTCAGC AAGGAACGTC ATCCATGCTC GAAGACGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GTGAACAAAC CCCAGAGAAG CCTCCCTCCT CATCTGCACC TCCAAGTGCT
    CCACAGTCCC TTCCAGAACC AGTGGCATCA GCACATTCCA GCAGTGCAGA GTCAGAAAGC
    ACCAGTGACT CAGACAGTTC CTCAGACTCA GAGAGCGAGA GCAGTTCAAG TGACAGCGAA
    GAAAATGAGC CCCTAGAAAC CCCAGCTCCG GAGCCTGAGC CTCCAACAAC AAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCAGCTG CGCCACCAGA GGGCCCCAGG
    AGCACAGAGC CCCCACGGCG GCACCCAGAG AGTAAGGGCA GCAGCGACAG TGCCACGAGT
    CAGGAGCATT CTGAATCCAA AGATCCTCCC CCTAAAAGCT CCAGCAAAGC CCCCCGGGCC
    CCACCCGAAG CCCCCCACCC CGGAAAGAGG AGCTGTCAGA AGTCTCCGGC ACAGCAGGAG
    CCCCCACAAA GGCAAACCGT TGGAACCAAA CAACCCAAAA AACCTGTCAA GGCCTCTGCC
    CGGGCAGGTT CACGGACCAG CCTGCAGGGG GAAAGGGAGC CAGGGCTTCT TCCCTATGGC
    TCCCGAGACC AGACTTCCAA AGACAAGCCC AAGGTGAAGA CGAAAGGACG GCCCCGGGCC
    GCAGCAAGCA ACGAACCCAA GCCAGCAGTG CCCCCCTCCA GTGAGAAGAA GAAGCACAAG
    AGCTCCCTCC CTGCCCCCTC TAAGGCTCTC TCAGGCCCAG AACCCGCGAA GGACAATGTG
    GAGGACAGGA CCCCTGAGCA CTTTGCTCTT GTTCCCCTGA CTGAGAGCCA GGGCCCACCC
    CACAGTGGCA GCGGCAGCAG GACTAGTGGC TGCCGCCAAG CCGTGGTGGT CCAGGAGGAC
    AGCCGCAAAG ACAGACTCCC ATTGCCTTTG AGAGACACCA AGCTGCTCTC ACCGCTCAGG
    GACACTCCTC CCCCACAAAG CTTGATGGTG AAGATCACCC TAGACCTGCT CTCTCGGATA
    CCCCAGCCTC CCGGGAAGGG GAGCCGCCAG AGGAAAGCAG AAGATAAACA GCCGCCCGCA
    GGGAAGAAGC ACAGCTCTGA GAAGAGGAGC TCAGACAGCT CAAGCAAGTT GGCCAAAAAG
    AGAAAGGGTG AAGCAGAAAG AGACTGTGAT AACAAGAAAA TCAGACTGGA GAAGGAAATC
    AAATCACAGT CATCTTCATC TTCATCCTCC CACAAAGAAT CTTCTAAAAC AAAGCCCTCC
    AGGCCCTCCT CACAGTCCTC AAAGAAGGAA ATGCTCCCCC CGCCACCCGT GTCCTCGTCC
    TCCCAGAAGC CAGCCAAGCC TGCACTTAAG AGGTCAAGGC GGGAAGCAGA CACCTGTGGC
    CAGGACCCTC CCAAAAGTGC CAGCAGTACC AAGAGCAACC ACAAAGACTC TTCCATTCCC
    AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC AGAAGCTCCT CGGAGCACAA GGGTTCTTCC
    GGAGATACTG CAAATCCTTT TCCAGTGCCT TCTTTGCCAA ATGGTAACTC TAAACCAGGG
    AAGCCTCAAG TGAAGTTTGA CAAACAACAA GCAGACCTTC ACATGAGGGA GGCAAAAAAG
    ATGAAGCAGA AAGCAGAGTT AATGACGGAC AGGGTTGGAA AGGCTTTTAA GTACCTGGAA
    GCCGTCTTGT CCTTCATTGA GTGCGGAATT GCCACAGAGT CTGAAAGCCA GTCATCCAAG
    TCAGCTTACT CTGTCTACTC AGAAACTGTA GATCTCATTA AATTCATAAT GTCATTAAAA
    TCCTTCTCAG ATGCCACAGC GCCAACACAA GAGAAAATAT TTGCTGTTTT ATGCATGCGT
    TGCCAGTCCA TTTTGAACAT GGCGATGTTT CGTTGTAAAA AAGACATAGC AATAAAGTAT
    TCTCGTACTC TTAATAAACA CTTCGAGAGT TCTTCCAAAG TCGCCCAGGC ACCTTCTCCA
    TGCATTGCAA GAAGCACAGG CACACCATCC CCTCTTTCCC CAATGCCTTC TCCTGCCAGC
    TCCGTAGGGT CCCAGTCAAG TGCTGGCAGT GTGGGGAGCA GTGGGGTGGC TGCCACTATC
    AGCACCCCAG TCACCATCCA GAATATGACA TCTTCCTATG TCACCATCAC ATCCCATGTT
    CTTACCGCCT TTGACCTTTG GGAACAGGCC GAGGCCCTCA CGAGGAAGAA TAAAGAATTC
    TTTGCTCGGC TCAGCACAAA TGTGTGCACC TTGGCCCTCA ACAGCAGTTT GGTGGACCTG
    GTGCACTATA CACGACAGGG TTTTCAGCAG CTACAAGAAT TAACCAAAAC ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25298
    Clone ID Related Accession (Same CDS sequence) XM_011531973.3 , NM_001166693.2 , XM_005263007.3 , XM_011531973.2
    Accession Version XM_005263007.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263007.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCATTTA CAGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA
    TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG
    TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT
    AAGTCTCACA CTCATCGCCT GGATGCTTCT GAAAATAGGT TGGGAAAGCC GAAATATCCT
    TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC
    CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA
    AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGCGGCCCA
    CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT
    CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGATTCGGCT
    CCAGAGAGGG AGCTTTCTCC CTTAATCTCT TTGCCTTCCC CAGTTCCCCC TTTGTCACCT
    ATACATTCCA ACCAGCAAAC TCTTCCCCGG ACGCAAGGAA GCAGCAAGGT TCATGGCAGC
    AGCAATAACA GTAAAGGCTA TTGCCCAGCC AAATCTCCCA AGGACCTAGC AGTGAAAGTC
    CATGATAAAG AGACCCCTCA AGACAGTTTG GTGGCCCCTG CCCAGCCGCC TTCTCAGACA
    TTTCCACCTC CCTCCCTCCC CTCAAAAAGT GTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGATGGTCA AGATCAGGCC CCTAGTGAAT CCCCTGAACT GAAACCACTG
    CCGGAGGACT ATCGACAGCA GACCTTTGAA AAAACAGACT TGAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCTTCTCAG TCAGTTGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCATTCAT GGCCGCCTCC TTTGACAGCA
    ATACATACGC CTAGTACAGC TGAGCCATCC AAGTTTCCTT TCCCTACAAA GGACTCTCAG
    CATGTCAGTT CTGTAACCCA AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACTCA
    AATTCTCAGC AAGGAACGTC ATCCATGCTC GAAGACGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GTGAACAAAC CCCAGAGAAG CCTCCCTCCT CATCTGCACC TCCAAGTGCT
    CCACAGTCCC TTCCAGAACC AGTGGCATCA GCACATTCCA GCAGTGCAGA GTCAGAAAGC
    ACCAGTGACT CAGACAGTTC CTCAGACTCA GAGAGCGAGA GCAGTTCAAG TGACAGCGAA
    GAAAATGAGC CCCTAGAAAC CCCAGCTCCG GAGCCTGAGC CTCCAACAAC AAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCAGCTG CGCCACCAGA GGGCCCCAGG
    AGCACAGAGC CCCCACGGCG GCACCCAGAG AGTAAGGGCA GCAGCGACAG TGCCACGAGT
    CAGGAGCATT CTGAATCCAA AGATCCTCCC CCTAAAAGCT CCAGCAAAGC CCCCCGGGCC
    CCACCCGAAG CCCCCCACCC CGGAAAGAGG AGCTGTCAGA AGTCTCCGGC ACAGCAGGAG
    CCCCCACAAA GGCAAACCGT TGGAACCAAA CAACCCAAAA AACCTGTCAA GGCCTCTGCC
    CGGGCAGGTT CACGGACCAG CCTGCAGGGG GAAAGGGAGC CAGGGCTTCT TCCCTATGGC
    TCCCGAGACC AGACTTCCAA AGACAAGCCC AAGGTGAAGA CGAAAGGACG GCCCCGGGCC
    GCAGCAAGCA ACGAACCCAA GCCAGCAGTG CCCCCCTCCA GTGAGAAGAA GAAGCACAAG
    AGCTCCCTCC CTGCCCCCTC TAAGGCTCTC TCAGGCCCAG AACCCGCGAA GGACAATGTG
    GAGGACAGGA CCCCTGAGCA CTTTGCTCTT GTTCCCCTGA CTGAGAGCCA GGGCCCACCC
    CACAGTGGCA GCGGCAGCAG GACTAGTGGC TGCCGCCAAG CCGTGGTGGT CCAGGAGGAC
    AGCCGCAAAG ACAGACTCCC ATTGCCTTTG AGAGACACCA AGCTGCTCTC ACCGCTCAGG
    GACACTCCTC CCCCACAAAG CTTGATGGTG AAGATCACCC TAGACCTGCT CTCTCGGATA
    CCCCAGCCTC CCGGGAAGGG GAGCCGCCAG AGGAAAGCAG AAGATAAACA GCCGCCCGCA
    GGGAAGAAGC ACAGCTCTGA GAAGAGGAGC TCAGACAGCT CAAGCAAGTT GGCCAAAAAG
    AGAAAGGGTG AAGCAGAAAG AGACTGTGAT AACAAGAAAA TCAGACTGGA GAAGGAAATC
    AAATCACAGT CATCTTCATC TTCATCCTCC CACAAAGAAT CTTCTAAAAC AAAGCCCTCC
    AGGCCCTCCT CACAGTCCTC AAAGAAGGAA ATGCTCCCCC CGCCACCCGT GTCCTCGTCC
    TCCCAGAAGC CAGCCAAGCC TGCACTTAAG AGGTCAAGGC GGGAAGCAGA CACCTGTGGC
    CAGGACCCTC CCAAAAGTGC CAGCAGTACC AAGAGCAACC ACAAAGACTC TTCCATTCCC
    AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC AGAAGCTCCT CGGAGCACAA GGGTTCTTCC
    GGAGATACTG CAAATCCTTT TCCAGTGCCT TCTTTGCCAA ATGGTAACTC TAAACCAGGG
    AAGCCTCAAG TGAAGTTTGA CAAACAACAA GCAGACCTTC ACATGAGGGA GGCAAAAAAG
    ATGAAGCAGA AAGCAGAGTT AATGACGGAC AGGGTTGGAA AGGCTTTTAA GTACCTGGAA
    GCCGTCTTGT CCTTCATTGA GTGCGGAATT GCCACAGAGT CTGAAAGCCA GTCATCCAAG
    TCAGCTTACT CTGTCTACTC AGAAACTGTA GATCTCATTA AATTCATAAT GTCATTAAAA
    TCCTTCTCAG ATGCCACAGC GCCAACACAA GAGAAAATAT TTGCTGTTTT ATGCATGCGT
    TGCCAGTCCA TTTTGAACAT GGCGATGTTT CGTTGTAAAA AAGACATAGC AATAAAGTAT
    TCTCGTACTC TTAATAAACA CTTCGAGAGT TCTTCCAAAG TCGCCCAGGC ACCTTCTCCA
    TGCATTGCAA GAAGCACAGG CACACCATCC CCTCTTTCCC CAATGCCTTC TCCTGCCAGC
    TCCGTAGGGT CCCAGTCAAG TGCTGGCAGT GTGGGGAGCA GTGGGGTGGC TGCCACTATC
    AGCACCCCAG TCACCATCCA GAATATGACA TCTTCCTATG TCACCATCAC ATCCCATGTT
    CTTACCGCCT TTGACCTTTG GGAACAGGCC GAGGCCCTCA CGAGGAAGAA TAAAGAATTC
    TTTGCTCGGC TCAGCACAAA TGTGTGCACC TTGGCCCTCA ACAGCAGTTT GGTGGACCTG
    GTGCACTATA CACGACAGGG TTTTCAGCAG CTACAAGAAT TAACCAAAAC ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263064.1
    CDS367..4023
    Translation

    Target ORF information:

    RefSeq Version XM_005263007.3
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263007.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCATTTA CAGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA
    TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG
    TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT
    AAGTCTCACA CTCATCGCCT GGATGCTTCT GAAAATAGGT TGGGAAAGCC GAAATATCCT
    TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC
    CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA
    AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGCGGCCCA
    CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT
    CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGATTCGGCT
    CCAGAGAGGG AGCTTTCTCC CTTAATCTCT TTGCCTTCCC CAGTTCCCCC TTTGTCACCT
    ATACATTCCA ACCAGCAAAC TCTTCCCCGG ACGCAAGGAA GCAGCAAGGT TCATGGCAGC
    AGCAATAACA GTAAAGGCTA TTGCCCAGCC AAATCTCCCA AGGACCTAGC AGTGAAAGTC
    CATGATAAAG AGACCCCTCA AGACAGTTTG GTGGCCCCTG CCCAGCCGCC TTCTCAGACA
    TTTCCACCTC CCTCCCTCCC CTCAAAAAGT GTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGATGGTCA AGATCAGGCC CCTAGTGAAT CCCCTGAACT GAAACCACTG
    CCGGAGGACT ATCGACAGCA GACCTTTGAA AAAACAGACT TGAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCTTCTCAG TCAGTTGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCATTCAT GGCCGCCTCC TTTGACAGCA
    ATACATACGC CTAGTACAGC TGAGCCATCC AAGTTTCCTT TCCCTACAAA GGACTCTCAG
    CATGTCAGTT CTGTAACCCA AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACTCA
    AATTCTCAGC AAGGAACGTC ATCCATGCTC GAAGACGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GTGAACAAAC CCCAGAGAAG CCTCCCTCCT CATCTGCACC TCCAAGTGCT
    CCACAGTCCC TTCCAGAACC AGTGGCATCA GCACATTCCA GCAGTGCAGA GTCAGAAAGC
    ACCAGTGACT CAGACAGTTC CTCAGACTCA GAGAGCGAGA GCAGTTCAAG TGACAGCGAA
    GAAAATGAGC CCCTAGAAAC CCCAGCTCCG GAGCCTGAGC CTCCAACAAC AAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCAGCTG CGCCACCAGA GGGCCCCAGG
    AGCACAGAGC CCCCACGGCG GCACCCAGAG AGTAAGGGCA GCAGCGACAG TGCCACGAGT
    CAGGAGCATT CTGAATCCAA AGATCCTCCC CCTAAAAGCT CCAGCAAAGC CCCCCGGGCC
    CCACCCGAAG CCCCCCACCC CGGAAAGAGG AGCTGTCAGA AGTCTCCGGC ACAGCAGGAG
    CCCCCACAAA GGCAAACCGT TGGAACCAAA CAACCCAAAA AACCTGTCAA GGCCTCTGCC
    CGGGCAGGTT CACGGACCAG CCTGCAGGGG GAAAGGGAGC CAGGGCTTCT TCCCTATGGC
    TCCCGAGACC AGACTTCCAA AGACAAGCCC AAGGTGAAGA CGAAAGGACG GCCCCGGGCC
    GCAGCAAGCA ACGAACCCAA GCCAGCAGTG CCCCCCTCCA GTGAGAAGAA GAAGCACAAG
    AGCTCCCTCC CTGCCCCCTC TAAGGCTCTC TCAGGCCCAG AACCCGCGAA GGACAATGTG
    GAGGACAGGA CCCCTGAGCA CTTTGCTCTT GTTCCCCTGA CTGAGAGCCA GGGCCCACCC
    CACAGTGGCA GCGGCAGCAG GACTAGTGGC TGCCGCCAAG CCGTGGTGGT CCAGGAGGAC
    AGCCGCAAAG ACAGACTCCC ATTGCCTTTG AGAGACACCA AGCTGCTCTC ACCGCTCAGG
    GACACTCCTC CCCCACAAAG CTTGATGGTG AAGATCACCC TAGACCTGCT CTCTCGGATA
    CCCCAGCCTC CCGGGAAGGG GAGCCGCCAG AGGAAAGCAG AAGATAAACA GCCGCCCGCA
    GGGAAGAAGC ACAGCTCTGA GAAGAGGAGC TCAGACAGCT CAAGCAAGTT GGCCAAAAAG
    AGAAAGGGTG AAGCAGAAAG AGACTGTGAT AACAAGAAAA TCAGACTGGA GAAGGAAATC
    AAATCACAGT CATCTTCATC TTCATCCTCC CACAAAGAAT CTTCTAAAAC AAAGCCCTCC
    AGGCCCTCCT CACAGTCCTC AAAGAAGGAA ATGCTCCCCC CGCCACCCGT GTCCTCGTCC
    TCCCAGAAGC CAGCCAAGCC TGCACTTAAG AGGTCAAGGC GGGAAGCAGA CACCTGTGGC
    CAGGACCCTC CCAAAAGTGC CAGCAGTACC AAGAGCAACC ACAAAGACTC TTCCATTCCC
    AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC AGAAGCTCCT CGGAGCACAA GGGTTCTTCC
    GGAGATACTG CAAATCCTTT TCCAGTGCCT TCTTTGCCAA ATGGTAACTC TAAACCAGGG
    AAGCCTCAAG TGAAGTTTGA CAAACAACAA GCAGACCTTC ACATGAGGGA GGCAAAAAAG
    ATGAAGCAGA AAGCAGAGTT AATGACGGAC AGGGTTGGAA AGGCTTTTAA GTACCTGGAA
    GCCGTCTTGT CCTTCATTGA GTGCGGAATT GCCACAGAGT CTGAAAGCCA GTCATCCAAG
    TCAGCTTACT CTGTCTACTC AGAAACTGTA GATCTCATTA AATTCATAAT GTCATTAAAA
    TCCTTCTCAG ATGCCACAGC GCCAACACAA GAGAAAATAT TTGCTGTTTT ATGCATGCGT
    TGCCAGTCCA TTTTGAACAT GGCGATGTTT CGTTGTAAAA AAGACATAGC AATAAAGTAT
    TCTCGTACTC TTAATAAACA CTTCGAGAGT TCTTCCAAAG TCGCCCAGGC ACCTTCTCCA
    TGCATTGCAA GAAGCACAGG CACACCATCC CCTCTTTCCC CAATGCCTTC TCCTGCCAGC
    TCCGTAGGGT CCCAGTCAAG TGCTGGCAGT GTGGGGAGCA GTGGGGTGGC TGCCACTATC
    AGCACCCCAG TCACCATCCA GAATATGACA TCTTCCTATG TCACCATCAC ATCCCATGTT
    CTTACCGCCT TTGACCTTTG GGAACAGGCC GAGGCCCTCA CGAGGAAGAA TAAAGAATTC
    TTTGCTCGGC TCAGCACAAA TGTGTGCACC TTGGCCCTCA ACAGCAGTTT GGTGGACCTG
    GTGCACTATA CACGACAGGG TTTTCAGCAG CTACAAGAAT TAACCAAAAC ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25298
    Clone ID Related Accession (Same CDS sequence) XM_011531973.3 , NM_001166693.2 , XM_005263007.3 , XM_011531973.2
    Accession Version XM_011531973.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011531973.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCATTTA CAGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA
    TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG
    TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT
    AAGTCTCACA CTCATCGCCT GGATGCTTCT GAAAATAGGT TGGGAAAGCC GAAATATCCT
    TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC
    CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA
    AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGCGGCCCA
    CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT
    CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGATTCGGCT
    CCAGAGAGGG AGCTTTCTCC CTTAATCTCT TTGCCTTCCC CAGTTCCCCC TTTGTCACCT
    ATACATTCCA ACCAGCAAAC TCTTCCCCGG ACGCAAGGAA GCAGCAAGGT TCATGGCAGC
    AGCAATAACA GTAAAGGCTA TTGCCCAGCC AAATCTCCCA AGGACCTAGC AGTGAAAGTC
    CATGATAAAG AGACCCCTCA AGACAGTTTG GTGGCCCCTG CCCAGCCGCC TTCTCAGACA
    TTTCCACCTC CCTCCCTCCC CTCAAAAAGT GTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGATGGTCA AGATCAGGCC CCTAGTGAAT CCCCTGAACT GAAACCACTG
    CCGGAGGACT ATCGACAGCA GACCTTTGAA AAAACAGACT TGAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCTTCTCAG TCAGTTGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCATTCAT GGCCGCCTCC TTTGACAGCA
    ATACATACGC CTAGTACAGC TGAGCCATCC AAGTTTCCTT TCCCTACAAA GGACTCTCAG
    CATGTCAGTT CTGTAACCCA AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACTCA
    AATTCTCAGC AAGGAACGTC ATCCATGCTC GAAGACGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GTGAACAAAC CCCAGAGAAG CCTCCCTCCT CATCTGCACC TCCAAGTGCT
    CCACAGTCCC TTCCAGAACC AGTGGCATCA GCACATTCCA GCAGTGCAGA GTCAGAAAGC
    ACCAGTGACT CAGACAGTTC CTCAGACTCA GAGAGCGAGA GCAGTTCAAG TGACAGCGAA
    GAAAATGAGC CCCTAGAAAC CCCAGCTCCG GAGCCTGAGC CTCCAACAAC AAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCAGCTG CGCCACCAGA GGGCCCCAGG
    AGCACAGAGC CCCCACGGCG GCACCCAGAG AGTAAGGGCA GCAGCGACAG TGCCACGAGT
    CAGGAGCATT CTGAATCCAA AGATCCTCCC CCTAAAAGCT CCAGCAAAGC CCCCCGGGCC
    CCACCCGAAG CCCCCCACCC CGGAAAGAGG AGCTGTCAGA AGTCTCCGGC ACAGCAGGAG
    CCCCCACAAA GGCAAACCGT TGGAACCAAA CAACCCAAAA AACCTGTCAA GGCCTCTGCC
    CGGGCAGGTT CACGGACCAG CCTGCAGGGG GAAAGGGAGC CAGGGCTTCT TCCCTATGGC
    TCCCGAGACC AGACTTCCAA AGACAAGCCC AAGGTGAAGA CGAAAGGACG GCCCCGGGCC
    GCAGCAAGCA ACGAACCCAA GCCAGCAGTG CCCCCCTCCA GTGAGAAGAA GAAGCACAAG
    AGCTCCCTCC CTGCCCCCTC TAAGGCTCTC TCAGGCCCAG AACCCGCGAA GGACAATGTG
    GAGGACAGGA CCCCTGAGCA CTTTGCTCTT GTTCCCCTGA CTGAGAGCCA GGGCCCACCC
    CACAGTGGCA GCGGCAGCAG GACTAGTGGC TGCCGCCAAG CCGTGGTGGT CCAGGAGGAC
    AGCCGCAAAG ACAGACTCCC ATTGCCTTTG AGAGACACCA AGCTGCTCTC ACCGCTCAGG
    GACACTCCTC CCCCACAAAG CTTGATGGTG AAGATCACCC TAGACCTGCT CTCTCGGATA
    CCCCAGCCTC CCGGGAAGGG GAGCCGCCAG AGGAAAGCAG AAGATAAACA GCCGCCCGCA
    GGGAAGAAGC ACAGCTCTGA GAAGAGGAGC TCAGACAGCT CAAGCAAGTT GGCCAAAAAG
    AGAAAGGGTG AAGCAGAAAG AGACTGTGAT AACAAGAAAA TCAGACTGGA GAAGGAAATC
    AAATCACAGT CATCTTCATC TTCATCCTCC CACAAAGAAT CTTCTAAAAC AAAGCCCTCC
    AGGCCCTCCT CACAGTCCTC AAAGAAGGAA ATGCTCCCCC CGCCACCCGT GTCCTCGTCC
    TCCCAGAAGC CAGCCAAGCC TGCACTTAAG AGGTCAAGGC GGGAAGCAGA CACCTGTGGC
    CAGGACCCTC CCAAAAGTGC CAGCAGTACC AAGAGCAACC ACAAAGACTC TTCCATTCCC
    AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC AGAAGCTCCT CGGAGCACAA GGGTTCTTCC
    GGAGATACTG CAAATCCTTT TCCAGTGCCT TCTTTGCCAA ATGGTAACTC TAAACCAGGG
    AAGCCTCAAG TGAAGTTTGA CAAACAACAA GCAGACCTTC ACATGAGGGA GGCAAAAAAG
    ATGAAGCAGA AAGCAGAGTT AATGACGGAC AGGGTTGGAA AGGCTTTTAA GTACCTGGAA
    GCCGTCTTGT CCTTCATTGA GTGCGGAATT GCCACAGAGT CTGAAAGCCA GTCATCCAAG
    TCAGCTTACT CTGTCTACTC AGAAACTGTA GATCTCATTA AATTCATAAT GTCATTAAAA
    TCCTTCTCAG ATGCCACAGC GCCAACACAA GAGAAAATAT TTGCTGTTTT ATGCATGCGT
    TGCCAGTCCA TTTTGAACAT GGCGATGTTT CGTTGTAAAA AAGACATAGC AATAAAGTAT
    TCTCGTACTC TTAATAAACA CTTCGAGAGT TCTTCCAAAG TCGCCCAGGC ACCTTCTCCA
    TGCATTGCAA GAAGCACAGG CACACCATCC CCTCTTTCCC CAATGCCTTC TCCTGCCAGC
    TCCGTAGGGT CCCAGTCAAG TGCTGGCAGT GTGGGGAGCA GTGGGGTGGC TGCCACTATC
    AGCACCCCAG TCACCATCCA GAATATGACA TCTTCCTATG TCACCATCAC ATCCCATGTT
    CTTACCGCCT TTGACCTTTG GGAACAGGCC GAGGCCCTCA CGAGGAAGAA TAAAGAATTC
    TTTGCTCGGC TCAGCACAAA TGTGTGCACC TTGGCCCTCA ACAGCAGTTT GGTGGACCTG
    GTGCACTATA CACGACAGGG TTTTCAGCAG CTACAAGAAT TAACCAAAAC ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011530275.1
    CDS189..3845
    Translation

    Target ORF information:

    RefSeq Version XM_011531973.2
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011531973.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCATTTA CAGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA
    TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG
    TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT
    AAGTCTCACA CTCATCGCCT GGATGCTTCT GAAAATAGGT TGGGAAAGCC GAAATATCCT
    TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC
    CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA
    AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGCGGCCCA
    CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT
    CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGATTCGGCT
    CCAGAGAGGG AGCTTTCTCC CTTAATCTCT TTGCCTTCCC CAGTTCCCCC TTTGTCACCT
    ATACATTCCA ACCAGCAAAC TCTTCCCCGG ACGCAAGGAA GCAGCAAGGT TCATGGCAGC
    AGCAATAACA GTAAAGGCTA TTGCCCAGCC AAATCTCCCA AGGACCTAGC AGTGAAAGTC
    CATGATAAAG AGACCCCTCA AGACAGTTTG GTGGCCCCTG CCCAGCCGCC TTCTCAGACA
    TTTCCACCTC CCTCCCTCCC CTCAAAAAGT GTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGATGGTCA AGATCAGGCC CCTAGTGAAT CCCCTGAACT GAAACCACTG
    CCGGAGGACT ATCGACAGCA GACCTTTGAA AAAACAGACT TGAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCTTCTCAG TCAGTTGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCATTCAT GGCCGCCTCC TTTGACAGCA
    ATACATACGC CTAGTACAGC TGAGCCATCC AAGTTTCCTT TCCCTACAAA GGACTCTCAG
    CATGTCAGTT CTGTAACCCA AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACTCA
    AATTCTCAGC AAGGAACGTC ATCCATGCTC GAAGACGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GTGAACAAAC CCCAGAGAAG CCTCCCTCCT CATCTGCACC TCCAAGTGCT
    CCACAGTCCC TTCCAGAACC AGTGGCATCA GCACATTCCA GCAGTGCAGA GTCAGAAAGC
    ACCAGTGACT CAGACAGTTC CTCAGACTCA GAGAGCGAGA GCAGTTCAAG TGACAGCGAA
    GAAAATGAGC CCCTAGAAAC CCCAGCTCCG GAGCCTGAGC CTCCAACAAC AAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCAGCTG CGCCACCAGA GGGCCCCAGG
    AGCACAGAGC CCCCACGGCG GCACCCAGAG AGTAAGGGCA GCAGCGACAG TGCCACGAGT
    CAGGAGCATT CTGAATCCAA AGATCCTCCC CCTAAAAGCT CCAGCAAAGC CCCCCGGGCC
    CCACCCGAAG CCCCCCACCC CGGAAAGAGG AGCTGTCAGA AGTCTCCGGC ACAGCAGGAG
    CCCCCACAAA GGCAAACCGT TGGAACCAAA CAACCCAAAA AACCTGTCAA GGCCTCTGCC
    CGGGCAGGTT CACGGACCAG CCTGCAGGGG GAAAGGGAGC CAGGGCTTCT TCCCTATGGC
    TCCCGAGACC AGACTTCCAA AGACAAGCCC AAGGTGAAGA CGAAAGGACG GCCCCGGGCC
    GCAGCAAGCA ACGAACCCAA GCCAGCAGTG CCCCCCTCCA GTGAGAAGAA GAAGCACAAG
    AGCTCCCTCC CTGCCCCCTC TAAGGCTCTC TCAGGCCCAG AACCCGCGAA GGACAATGTG
    GAGGACAGGA CCCCTGAGCA CTTTGCTCTT GTTCCCCTGA CTGAGAGCCA GGGCCCACCC
    CACAGTGGCA GCGGCAGCAG GACTAGTGGC TGCCGCCAAG CCGTGGTGGT CCAGGAGGAC
    AGCCGCAAAG ACAGACTCCC ATTGCCTTTG AGAGACACCA AGCTGCTCTC ACCGCTCAGG
    GACACTCCTC CCCCACAAAG CTTGATGGTG AAGATCACCC TAGACCTGCT CTCTCGGATA
    CCCCAGCCTC CCGGGAAGGG GAGCCGCCAG AGGAAAGCAG AAGATAAACA GCCGCCCGCA
    GGGAAGAAGC ACAGCTCTGA GAAGAGGAGC TCAGACAGCT CAAGCAAGTT GGCCAAAAAG
    AGAAAGGGTG AAGCAGAAAG AGACTGTGAT AACAAGAAAA TCAGACTGGA GAAGGAAATC
    AAATCACAGT CATCTTCATC TTCATCCTCC CACAAAGAAT CTTCTAAAAC AAAGCCCTCC
    AGGCCCTCCT CACAGTCCTC AAAGAAGGAA ATGCTCCCCC CGCCACCCGT GTCCTCGTCC
    TCCCAGAAGC CAGCCAAGCC TGCACTTAAG AGGTCAAGGC GGGAAGCAGA CACCTGTGGC
    CAGGACCCTC CCAAAAGTGC CAGCAGTACC AAGAGCAACC ACAAAGACTC TTCCATTCCC
    AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC AGAAGCTCCT CGGAGCACAA GGGTTCTTCC
    GGAGATACTG CAAATCCTTT TCCAGTGCCT TCTTTGCCAA ATGGTAACTC TAAACCAGGG
    AAGCCTCAAG TGAAGTTTGA CAAACAACAA GCAGACCTTC ACATGAGGGA GGCAAAAAAG
    ATGAAGCAGA AAGCAGAGTT AATGACGGAC AGGGTTGGAA AGGCTTTTAA GTACCTGGAA
    GCCGTCTTGT CCTTCATTGA GTGCGGAATT GCCACAGAGT CTGAAAGCCA GTCATCCAAG
    TCAGCTTACT CTGTCTACTC AGAAACTGTA GATCTCATTA AATTCATAAT GTCATTAAAA
    TCCTTCTCAG ATGCCACAGC GCCAACACAA GAGAAAATAT TTGCTGTTTT ATGCATGCGT
    TGCCAGTCCA TTTTGAACAT GGCGATGTTT CGTTGTAAAA AAGACATAGC AATAAAGTAT
    TCTCGTACTC TTAATAAACA CTTCGAGAGT TCTTCCAAAG TCGCCCAGGC ACCTTCTCCA
    TGCATTGCAA GAAGCACAGG CACACCATCC CCTCTTTCCC CAATGCCTTC TCCTGCCAGC
    TCCGTAGGGT CCCAGTCAAG TGCTGGCAGT GTGGGGAGCA GTGGGGTGGC TGCCACTATC
    AGCACCCCAG TCACCATCCA GAATATGACA TCTTCCTATG TCACCATCAC ATCCCATGTT
    CTTACCGCCT TTGACCTTTG GGAACAGGCC GAGGCCCTCA CGAGGAAGAA TAAAGAATTC
    TTTGCTCGGC TCAGCACAAA TGTGTGCACC TTGGCCCTCA ACAGCAGTTT GGTGGACCTG
    GTGCACTATA CACGACAGGG TTTTCAGCAG CTACAAGAAT TAACCAAAAC ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38940
    Clone ID Related Accession (Same CDS sequence) XM_005263009.4 , XM_005263009.3
    Accession Version XM_005263009.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263009.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCATTTA CAGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA
    TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG
    TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT
    AAGTCTCACA CTCATCGCCT GGATGCTTCT GAAAATAGGT TGGGAAAGCC GAAATATCCT
    TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC
    CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA
    AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGCGGCCCA
    CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT
    CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGATTCGGCT
    CCAGAGAGGG AGCTTTCTCC CTTAATCTCT TTGCCTTCCC CAGTTCCCCC TTTGTCACCT
    ATACATTCCA ACCAGCAAAC TCTTCCCCGG ACGCAAGGAA GCAGCAAGGT TCATGGCAGC
    AGCAATAACA GTAAAGGCTA TTGCCCAGCC AAATCTCCCA AGGACCTAGC AGTGAAAGTC
    CATGATAAAG AGACCCCTCA AGACAGTTTG GTGGCCCCTG CCCAGCCGCC TTCTCAGACA
    TTTCCACCTC CCTCCCTCCC CTCAAAAAGT GTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGATGGTCA AGATCAGGCC CCTAGTGAAT CCCCTGAACT GAAACCACTG
    CCGGAGGACT ATCGACAGCA GACCTTTGAA AAAACAGACT TGAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCTTCTCAG TCAGTTGAGA CCTACTCCAA TGAAGTCCAT
    TGTGTTGAAG AGATTCTGAA GGAAATGACC CATTCATGGC CGCCTCCTTT GACAGCAATA
    CATACGCCTA GTACAGCTGA GCCATCCAAG TTTCCTTTCC CTACAAAGGA CTCTCAGCAT
    GTCAGTTCTG TAACCCAAAA CCAAAAACAA TATGATACAT CTTCAAAAAC TCACTCAAAT
    TCTCAGCAAG GAACGTCATC CATGCTCGAA GACGACCTTC AGCTCAGTGA CAGTGAGGAC
    AGTGACAGTG AACAAACCCC AGAGAAGCCT CCCTCCTCAT CTGCACCTCC AAGTGCTCCA
    CAGTCCCTTC CAGAACCAGT GGCATCAGCA CATTCCAGCA GTGCAGAGTC AGAAAGCACC
    AGTGACTCAG ACAGTTCCTC AGACTCAGAG AGCGAGAGCA GTTCAAGTGA CAGCGAAGAA
    AATGAGCCCC TAGAAACCCC AGCTCCGGAG CCTGAGCCTC CAACAACAAA CAAATGGCAG
    CTGGACAACT GGCTGACCAA AGTCAGCCAG CCAGCTGCGC CACCAGAGGG CCCCAGGAGC
    ACAGAGCCCC CACGGCGGCA CCCAGAGAGT AAGGGCAGCA GCGACAGTGC CACGAGTCAG
    GAGCATTCTG AATCCAAAGA TCCTCCCCCT AAAAGCTCCA GCAAAGCCCC CCGGGCCCCA
    CCCGAAGCCC CCCACCCCGG AAAGAGGAGC TGTCAGAAGT CTCCGGCACA GCAGGAGCCC
    CCACAAAGGC AAACCGTTGG AACCAAACAA CCCAAAAAAC CTGTCAAGGC CTCTGCCCGG
    GCAGGTTCAC GGACCAGCCT GCAGGGGGAA AGGGAGCCAG GGCTTCTTCC CTATGGCTCC
    CGAGACCAGA CTTCCAAAGA CAAGCCCAAG GTGAAGACGA AAGGACGGCC CCGGGCCGCA
    GCAAGCAACG AACCCAAGCC AGCAGTGCCC CCCTCCAGTG AGAAGAAGAA GCACAAGAGC
    TCCCTCCCTG CCCCCTCTAA GGCTCTCTCA GGCCCAGAAC CCGCGAAGGA CAATGTGGAG
    GACAGGACCC CTGAGCACTT TGCTCTTGTT CCCCTGACTG AGAGCCAGGG CCCACCCCAC
    AGTGGCAGCG GCAGCAGGAC TAGTGGCTGC CGCCAAGCCG TGGTGGTCCA GGAGGACAGC
    CGCAAAGACA GACTCCCATT GCCTTTGAGA GACACCAAGC TGCTCTCACC GCTCAGGGAC
    ACTCCTCCCC CACAAAGCTT GATGGTGAAG ATCACCCTAG ACCTGCTCTC TCGGATACCC
    CAGCCTCCCG GGAAGGGGAG CCGCCAGAGG AAAGCAGAAG ATAAACAGCC GCCCGCAGGG
    AAGAAGCACA GCTCTGAGAA GAGGAGCTCA GACAGCTCAA GCAAGTTGGC CAAAAAGAGA
    AAGGGTGAAG CAGAAAGAGA CTGTGATAAC AAGAAAATCA GACTGGAGAA GGAAATCAAA
    TCACAGTCAT CTTCATCTTC ATCCTCCCAC AAAGAATCTT CTAAAACAAA GCCCTCCAGG
    CCCTCCTCAC AGTCCTCAAA GAAGGAAATG CTCCCCCCGC CACCCGTGTC CTCGTCCTCC
    CAGAAGCCAG CCAAGCCTGC ACTTAAGAGG TCAAGGCGGG AAGCAGACAC CTGTGGCCAG
    GACCCTCCCA AAAGTGCCAG CAGTACCAAG AGCAACCACA AAGACTCTTC CATTCCCAAG
    CAGAGAAGAG TAGAGGGGAA GGGCTCCAGA AGCTCCTCGG AGCACAAGGG TTCTTCCGGA
    GATACTGCAA ATCCTTTTCC AGTGCCTTCT TTGCCAAATG GTAACTCTAA ACCAGGGAAG
    CCTCAAGTGA AGTTTGACAA ACAACAAGCA GACCTTCACA TGAGGGAGGC AAAAAAGATG
    AAGCAGAAAG CAGAGTTAAT GACGGACAGG GTTGGAAAGG CTTTTAAGTA CCTGGAAGCC
    GTCTTGTCCT TCATTGAGTG CGGAATTGCC ACAGAGTCTG AAAGCCAGTC ATCCAAGTCA
    GCTTACTCTG TCTACTCAGA AACTGTAGAT CTCATTAAAT TCATAATGTC ATTAAAATCC
    TTCTCAGATG CCACAGCGCC AACACAAGAG AAAATATTTG CTGTTTTATG CATGCGTTGC
    CAGTCCATTT TGAACATGGC GATGTTTCGT TGTAAAAAAG ACATAGCAAT AAAGTATTCT
    CGTACTCTTA ATAAACACTT CGAGAGTTCT TCCAAAGTCG CCCAGGCACC TTCTCCATGC
    ATTGCAAGAA GCACAGGCAC ACCATCCCCT CTTTCCCCAA TGCCTTCTCC TGCCAGCTCC
    GTAGGGTCCC AGTCAAGTGC TGGCAGTGTG GGGAGCAGTG GGGTGGCTGC CACTATCAGC
    ACCCCAGTCA CCATCCAGAA TATGACATCT TCCTATGTCA CCATCACATC CCATGTTCTT
    ACCGCCTTTG ACCTTTGGGA ACAGGCCGAG GCCCTCACGA GGAAGAATAA AGAATTCTTT
    GCTCGGCTCA GCACAAATGT GTGCACCTTG GCCCTCAACA GCAGTTTGGT GGACCTGGTG
    CACTATACAC GACAGGGTTT TCAGCAGCTA CAAGAATTAA CCAAAACACC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263066.1
    CDS672..4325
    Translation

    Target ORF information:

    RefSeq Version XM_005263009.4
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263009.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCATTTA CAGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA
    TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG
    TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT
    AAGTCTCACA CTCATCGCCT GGATGCTTCT GAAAATAGGT TGGGAAAGCC GAAATATCCT
    TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC
    CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA
    AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGCGGCCCA
    CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT
    CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGATTCGGCT
    CCAGAGAGGG AGCTTTCTCC CTTAATCTCT TTGCCTTCCC CAGTTCCCCC TTTGTCACCT
    ATACATTCCA ACCAGCAAAC TCTTCCCCGG ACGCAAGGAA GCAGCAAGGT TCATGGCAGC
    AGCAATAACA GTAAAGGCTA TTGCCCAGCC AAATCTCCCA AGGACCTAGC AGTGAAAGTC
    CATGATAAAG AGACCCCTCA AGACAGTTTG GTGGCCCCTG CCCAGCCGCC TTCTCAGACA
    TTTCCACCTC CCTCCCTCCC CTCAAAAAGT GTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGATGGTCA AGATCAGGCC CCTAGTGAAT CCCCTGAACT GAAACCACTG
    CCGGAGGACT ATCGACAGCA GACCTTTGAA AAAACAGACT TGAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCTTCTCAG TCAGTTGAGA CCTACTCCAA TGAAGTCCAT
    TGTGTTGAAG AGATTCTGAA GGAAATGACC CATTCATGGC CGCCTCCTTT GACAGCAATA
    CATACGCCTA GTACAGCTGA GCCATCCAAG TTTCCTTTCC CTACAAAGGA CTCTCAGCAT
    GTCAGTTCTG TAACCCAAAA CCAAAAACAA TATGATACAT CTTCAAAAAC TCACTCAAAT
    TCTCAGCAAG GAACGTCATC CATGCTCGAA GACGACCTTC AGCTCAGTGA CAGTGAGGAC
    AGTGACAGTG AACAAACCCC AGAGAAGCCT CCCTCCTCAT CTGCACCTCC AAGTGCTCCA
    CAGTCCCTTC CAGAACCAGT GGCATCAGCA CATTCCAGCA GTGCAGAGTC AGAAAGCACC
    AGTGACTCAG ACAGTTCCTC AGACTCAGAG AGCGAGAGCA GTTCAAGTGA CAGCGAAGAA
    AATGAGCCCC TAGAAACCCC AGCTCCGGAG CCTGAGCCTC CAACAACAAA CAAATGGCAG
    CTGGACAACT GGCTGACCAA AGTCAGCCAG CCAGCTGCGC CACCAGAGGG CCCCAGGAGC
    ACAGAGCCCC CACGGCGGCA CCCAGAGAGT AAGGGCAGCA GCGACAGTGC CACGAGTCAG
    GAGCATTCTG AATCCAAAGA TCCTCCCCCT AAAAGCTCCA GCAAAGCCCC CCGGGCCCCA
    CCCGAAGCCC CCCACCCCGG AAAGAGGAGC TGTCAGAAGT CTCCGGCACA GCAGGAGCCC
    CCACAAAGGC AAACCGTTGG AACCAAACAA CCCAAAAAAC CTGTCAAGGC CTCTGCCCGG
    GCAGGTTCAC GGACCAGCCT GCAGGGGGAA AGGGAGCCAG GGCTTCTTCC CTATGGCTCC
    CGAGACCAGA CTTCCAAAGA CAAGCCCAAG GTGAAGACGA AAGGACGGCC CCGGGCCGCA
    GCAAGCAACG AACCCAAGCC AGCAGTGCCC CCCTCCAGTG AGAAGAAGAA GCACAAGAGC
    TCCCTCCCTG CCCCCTCTAA GGCTCTCTCA GGCCCAGAAC CCGCGAAGGA CAATGTGGAG
    GACAGGACCC CTGAGCACTT TGCTCTTGTT CCCCTGACTG AGAGCCAGGG CCCACCCCAC
    AGTGGCAGCG GCAGCAGGAC TAGTGGCTGC CGCCAAGCCG TGGTGGTCCA GGAGGACAGC
    CGCAAAGACA GACTCCCATT GCCTTTGAGA GACACCAAGC TGCTCTCACC GCTCAGGGAC
    ACTCCTCCCC CACAAAGCTT GATGGTGAAG ATCACCCTAG ACCTGCTCTC TCGGATACCC
    CAGCCTCCCG GGAAGGGGAG CCGCCAGAGG AAAGCAGAAG ATAAACAGCC GCCCGCAGGG
    AAGAAGCACA GCTCTGAGAA GAGGAGCTCA GACAGCTCAA GCAAGTTGGC CAAAAAGAGA
    AAGGGTGAAG CAGAAAGAGA CTGTGATAAC AAGAAAATCA GACTGGAGAA GGAAATCAAA
    TCACAGTCAT CTTCATCTTC ATCCTCCCAC AAAGAATCTT CTAAAACAAA GCCCTCCAGG
    CCCTCCTCAC AGTCCTCAAA GAAGGAAATG CTCCCCCCGC CACCCGTGTC CTCGTCCTCC
    CAGAAGCCAG CCAAGCCTGC ACTTAAGAGG TCAAGGCGGG AAGCAGACAC CTGTGGCCAG
    GACCCTCCCA AAAGTGCCAG CAGTACCAAG AGCAACCACA AAGACTCTTC CATTCCCAAG
    CAGAGAAGAG TAGAGGGGAA GGGCTCCAGA AGCTCCTCGG AGCACAAGGG TTCTTCCGGA
    GATACTGCAA ATCCTTTTCC AGTGCCTTCT TTGCCAAATG GTAACTCTAA ACCAGGGAAG
    CCTCAAGTGA AGTTTGACAA ACAACAAGCA GACCTTCACA TGAGGGAGGC AAAAAAGATG
    AAGCAGAAAG CAGAGTTAAT GACGGACAGG GTTGGAAAGG CTTTTAAGTA CCTGGAAGCC
    GTCTTGTCCT TCATTGAGTG CGGAATTGCC ACAGAGTCTG AAAGCCAGTC ATCCAAGTCA
    GCTTACTCTG TCTACTCAGA AACTGTAGAT CTCATTAAAT TCATAATGTC ATTAAAATCC
    TTCTCAGATG CCACAGCGCC AACACAAGAG AAAATATTTG CTGTTTTATG CATGCGTTGC
    CAGTCCATTT TGAACATGGC GATGTTTCGT TGTAAAAAAG ACATAGCAAT AAAGTATTCT
    CGTACTCTTA ATAAACACTT CGAGAGTTCT TCCAAAGTCG CCCAGGCACC TTCTCCATGC
    ATTGCAAGAA GCACAGGCAC ACCATCCCCT CTTTCCCCAA TGCCTTCTCC TGCCAGCTCC
    GTAGGGTCCC AGTCAAGTGC TGGCAGTGTG GGGAGCAGTG GGGTGGCTGC CACTATCAGC
    ACCCCAGTCA CCATCCAGAA TATGACATCT TCCTATGTCA CCATCACATC CCATGTTCTT
    ACCGCCTTTG ACCTTTGGGA ACAGGCCGAG GCCCTCACGA GGAAGAATAA AGAATTCTTT
    GCTCGGCTCA GCACAAATGT GTGCACCTTG GCCCTCAACA GCAGTTTGGT GGACCTGGTG
    CACTATACAC GACAGGGTTT TCAGCAGCTA CAAGAATTAA CCAAAACACC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38940
    Clone ID Related Accession (Same CDS sequence) XM_005263009.4 , XM_005263009.3
    Accession Version XM_005263009.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263009.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCATTTA CAGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA
    TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG
    TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT
    AAGTCTCACA CTCATCGCCT GGATGCTTCT GAAAATAGGT TGGGAAAGCC GAAATATCCT
    TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC
    CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA
    AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGCGGCCCA
    CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT
    CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGATTCGGCT
    CCAGAGAGGG AGCTTTCTCC CTTAATCTCT TTGCCTTCCC CAGTTCCCCC TTTGTCACCT
    ATACATTCCA ACCAGCAAAC TCTTCCCCGG ACGCAAGGAA GCAGCAAGGT TCATGGCAGC
    AGCAATAACA GTAAAGGCTA TTGCCCAGCC AAATCTCCCA AGGACCTAGC AGTGAAAGTC
    CATGATAAAG AGACCCCTCA AGACAGTTTG GTGGCCCCTG CCCAGCCGCC TTCTCAGACA
    TTTCCACCTC CCTCCCTCCC CTCAAAAAGT GTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGATGGTCA AGATCAGGCC CCTAGTGAAT CCCCTGAACT GAAACCACTG
    CCGGAGGACT ATCGACAGCA GACCTTTGAA AAAACAGACT TGAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCTTCTCAG TCAGTTGAGA CCTACTCCAA TGAAGTCCAT
    TGTGTTGAAG AGATTCTGAA GGAAATGACC CATTCATGGC CGCCTCCTTT GACAGCAATA
    CATACGCCTA GTACAGCTGA GCCATCCAAG TTTCCTTTCC CTACAAAGGA CTCTCAGCAT
    GTCAGTTCTG TAACCCAAAA CCAAAAACAA TATGATACAT CTTCAAAAAC TCACTCAAAT
    TCTCAGCAAG GAACGTCATC CATGCTCGAA GACGACCTTC AGCTCAGTGA CAGTGAGGAC
    AGTGACAGTG AACAAACCCC AGAGAAGCCT CCCTCCTCAT CTGCACCTCC AAGTGCTCCA
    CAGTCCCTTC CAGAACCAGT GGCATCAGCA CATTCCAGCA GTGCAGAGTC AGAAAGCACC
    AGTGACTCAG ACAGTTCCTC AGACTCAGAG AGCGAGAGCA GTTCAAGTGA CAGCGAAGAA
    AATGAGCCCC TAGAAACCCC AGCTCCGGAG CCTGAGCCTC CAACAACAAA CAAATGGCAG
    CTGGACAACT GGCTGACCAA AGTCAGCCAG CCAGCTGCGC CACCAGAGGG CCCCAGGAGC
    ACAGAGCCCC CACGGCGGCA CCCAGAGAGT AAGGGCAGCA GCGACAGTGC CACGAGTCAG
    GAGCATTCTG AATCCAAAGA TCCTCCCCCT AAAAGCTCCA GCAAAGCCCC CCGGGCCCCA
    CCCGAAGCCC CCCACCCCGG AAAGAGGAGC TGTCAGAAGT CTCCGGCACA GCAGGAGCCC
    CCACAAAGGC AAACCGTTGG AACCAAACAA CCCAAAAAAC CTGTCAAGGC CTCTGCCCGG
    GCAGGTTCAC GGACCAGCCT GCAGGGGGAA AGGGAGCCAG GGCTTCTTCC CTATGGCTCC
    CGAGACCAGA CTTCCAAAGA CAAGCCCAAG GTGAAGACGA AAGGACGGCC CCGGGCCGCA
    GCAAGCAACG AACCCAAGCC AGCAGTGCCC CCCTCCAGTG AGAAGAAGAA GCACAAGAGC
    TCCCTCCCTG CCCCCTCTAA GGCTCTCTCA GGCCCAGAAC CCGCGAAGGA CAATGTGGAG
    GACAGGACCC CTGAGCACTT TGCTCTTGTT CCCCTGACTG AGAGCCAGGG CCCACCCCAC
    AGTGGCAGCG GCAGCAGGAC TAGTGGCTGC CGCCAAGCCG TGGTGGTCCA GGAGGACAGC
    CGCAAAGACA GACTCCCATT GCCTTTGAGA GACACCAAGC TGCTCTCACC GCTCAGGGAC
    ACTCCTCCCC CACAAAGCTT GATGGTGAAG ATCACCCTAG ACCTGCTCTC TCGGATACCC
    CAGCCTCCCG GGAAGGGGAG CCGCCAGAGG AAAGCAGAAG ATAAACAGCC GCCCGCAGGG
    AAGAAGCACA GCTCTGAGAA GAGGAGCTCA GACAGCTCAA GCAAGTTGGC CAAAAAGAGA
    AAGGGTGAAG CAGAAAGAGA CTGTGATAAC AAGAAAATCA GACTGGAGAA GGAAATCAAA
    TCACAGTCAT CTTCATCTTC ATCCTCCCAC AAAGAATCTT CTAAAACAAA GCCCTCCAGG
    CCCTCCTCAC AGTCCTCAAA GAAGGAAATG CTCCCCCCGC CACCCGTGTC CTCGTCCTCC
    CAGAAGCCAG CCAAGCCTGC ACTTAAGAGG TCAAGGCGGG AAGCAGACAC CTGTGGCCAG
    GACCCTCCCA AAAGTGCCAG CAGTACCAAG AGCAACCACA AAGACTCTTC CATTCCCAAG
    CAGAGAAGAG TAGAGGGGAA GGGCTCCAGA AGCTCCTCGG AGCACAAGGG TTCTTCCGGA
    GATACTGCAA ATCCTTTTCC AGTGCCTTCT TTGCCAAATG GTAACTCTAA ACCAGGGAAG
    CCTCAAGTGA AGTTTGACAA ACAACAAGCA GACCTTCACA TGAGGGAGGC AAAAAAGATG
    AAGCAGAAAG CAGAGTTAAT GACGGACAGG GTTGGAAAGG CTTTTAAGTA CCTGGAAGCC
    GTCTTGTCCT TCATTGAGTG CGGAATTGCC ACAGAGTCTG AAAGCCAGTC ATCCAAGTCA
    GCTTACTCTG TCTACTCAGA AACTGTAGAT CTCATTAAAT TCATAATGTC ATTAAAATCC
    TTCTCAGATG CCACAGCGCC AACACAAGAG AAAATATTTG CTGTTTTATG CATGCGTTGC
    CAGTCCATTT TGAACATGGC GATGTTTCGT TGTAAAAAAG ACATAGCAAT AAAGTATTCT
    CGTACTCTTA ATAAACACTT CGAGAGTTCT TCCAAAGTCG CCCAGGCACC TTCTCCATGC
    ATTGCAAGAA GCACAGGCAC ACCATCCCCT CTTTCCCCAA TGCCTTCTCC TGCCAGCTCC
    GTAGGGTCCC AGTCAAGTGC TGGCAGTGTG GGGAGCAGTG GGGTGGCTGC CACTATCAGC
    ACCCCAGTCA CCATCCAGAA TATGACATCT TCCTATGTCA CCATCACATC CCATGTTCTT
    ACCGCCTTTG ACCTTTGGGA ACAGGCCGAG GCCCTCACGA GGAAGAATAA AGAATTCTTT
    GCTCGGCTCA GCACAAATGT GTGCACCTTG GCCCTCAACA GCAGTTTGGT GGACCTGGTG
    CACTATACAC GACAGGGTTT TCAGCAGCTA CAAGAATTAA CCAAAACACC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263066.1
    CDS271..3924
    Translation

    Target ORF information:

    RefSeq Version XM_005263009.3
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263009.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCATTTA CAGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA
    TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG
    TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT
    AAGTCTCACA CTCATCGCCT GGATGCTTCT GAAAATAGGT TGGGAAAGCC GAAATATCCT
    TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC
    CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA
    AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGCGGCCCA
    CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT
    CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGATTCGGCT
    CCAGAGAGGG AGCTTTCTCC CTTAATCTCT TTGCCTTCCC CAGTTCCCCC TTTGTCACCT
    ATACATTCCA ACCAGCAAAC TCTTCCCCGG ACGCAAGGAA GCAGCAAGGT TCATGGCAGC
    AGCAATAACA GTAAAGGCTA TTGCCCAGCC AAATCTCCCA AGGACCTAGC AGTGAAAGTC
    CATGATAAAG AGACCCCTCA AGACAGTTTG GTGGCCCCTG CCCAGCCGCC TTCTCAGACA
    TTTCCACCTC CCTCCCTCCC CTCAAAAAGT GTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGATGGTCA AGATCAGGCC CCTAGTGAAT CCCCTGAACT GAAACCACTG
    CCGGAGGACT ATCGACAGCA GACCTTTGAA AAAACAGACT TGAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCTTCTCAG TCAGTTGAGA CCTACTCCAA TGAAGTCCAT
    TGTGTTGAAG AGATTCTGAA GGAAATGACC CATTCATGGC CGCCTCCTTT GACAGCAATA
    CATACGCCTA GTACAGCTGA GCCATCCAAG TTTCCTTTCC CTACAAAGGA CTCTCAGCAT
    GTCAGTTCTG TAACCCAAAA CCAAAAACAA TATGATACAT CTTCAAAAAC TCACTCAAAT
    TCTCAGCAAG GAACGTCATC CATGCTCGAA GACGACCTTC AGCTCAGTGA CAGTGAGGAC
    AGTGACAGTG AACAAACCCC AGAGAAGCCT CCCTCCTCAT CTGCACCTCC AAGTGCTCCA
    CAGTCCCTTC CAGAACCAGT GGCATCAGCA CATTCCAGCA GTGCAGAGTC AGAAAGCACC
    AGTGACTCAG ACAGTTCCTC AGACTCAGAG AGCGAGAGCA GTTCAAGTGA CAGCGAAGAA
    AATGAGCCCC TAGAAACCCC AGCTCCGGAG CCTGAGCCTC CAACAACAAA CAAATGGCAG
    CTGGACAACT GGCTGACCAA AGTCAGCCAG CCAGCTGCGC CACCAGAGGG CCCCAGGAGC
    ACAGAGCCCC CACGGCGGCA CCCAGAGAGT AAGGGCAGCA GCGACAGTGC CACGAGTCAG
    GAGCATTCTG AATCCAAAGA TCCTCCCCCT AAAAGCTCCA GCAAAGCCCC CCGGGCCCCA
    CCCGAAGCCC CCCACCCCGG AAAGAGGAGC TGTCAGAAGT CTCCGGCACA GCAGGAGCCC
    CCACAAAGGC AAACCGTTGG AACCAAACAA CCCAAAAAAC CTGTCAAGGC CTCTGCCCGG
    GCAGGTTCAC GGACCAGCCT GCAGGGGGAA AGGGAGCCAG GGCTTCTTCC CTATGGCTCC
    CGAGACCAGA CTTCCAAAGA CAAGCCCAAG GTGAAGACGA AAGGACGGCC CCGGGCCGCA
    GCAAGCAACG AACCCAAGCC AGCAGTGCCC CCCTCCAGTG AGAAGAAGAA GCACAAGAGC
    TCCCTCCCTG CCCCCTCTAA GGCTCTCTCA GGCCCAGAAC CCGCGAAGGA CAATGTGGAG
    GACAGGACCC CTGAGCACTT TGCTCTTGTT CCCCTGACTG AGAGCCAGGG CCCACCCCAC
    AGTGGCAGCG GCAGCAGGAC TAGTGGCTGC CGCCAAGCCG TGGTGGTCCA GGAGGACAGC
    CGCAAAGACA GACTCCCATT GCCTTTGAGA GACACCAAGC TGCTCTCACC GCTCAGGGAC
    ACTCCTCCCC CACAAAGCTT GATGGTGAAG ATCACCCTAG ACCTGCTCTC TCGGATACCC
    CAGCCTCCCG GGAAGGGGAG CCGCCAGAGG AAAGCAGAAG ATAAACAGCC GCCCGCAGGG
    AAGAAGCACA GCTCTGAGAA GAGGAGCTCA GACAGCTCAA GCAAGTTGGC CAAAAAGAGA
    AAGGGTGAAG CAGAAAGAGA CTGTGATAAC AAGAAAATCA GACTGGAGAA GGAAATCAAA
    TCACAGTCAT CTTCATCTTC ATCCTCCCAC AAAGAATCTT CTAAAACAAA GCCCTCCAGG
    CCCTCCTCAC AGTCCTCAAA GAAGGAAATG CTCCCCCCGC CACCCGTGTC CTCGTCCTCC
    CAGAAGCCAG CCAAGCCTGC ACTTAAGAGG TCAAGGCGGG AAGCAGACAC CTGTGGCCAG
    GACCCTCCCA AAAGTGCCAG CAGTACCAAG AGCAACCACA AAGACTCTTC CATTCCCAAG
    CAGAGAAGAG TAGAGGGGAA GGGCTCCAGA AGCTCCTCGG AGCACAAGGG TTCTTCCGGA
    GATACTGCAA ATCCTTTTCC AGTGCCTTCT TTGCCAAATG GTAACTCTAA ACCAGGGAAG
    CCTCAAGTGA AGTTTGACAA ACAACAAGCA GACCTTCACA TGAGGGAGGC AAAAAAGATG
    AAGCAGAAAG CAGAGTTAAT GACGGACAGG GTTGGAAAGG CTTTTAAGTA CCTGGAAGCC
    GTCTTGTCCT TCATTGAGTG CGGAATTGCC ACAGAGTCTG AAAGCCAGTC ATCCAAGTCA
    GCTTACTCTG TCTACTCAGA AACTGTAGAT CTCATTAAAT TCATAATGTC ATTAAAATCC
    TTCTCAGATG CCACAGCGCC AACACAAGAG AAAATATTTG CTGTTTTATG CATGCGTTGC
    CAGTCCATTT TGAACATGGC GATGTTTCGT TGTAAAAAAG ACATAGCAAT AAAGTATTCT
    CGTACTCTTA ATAAACACTT CGAGAGTTCT TCCAAAGTCG CCCAGGCACC TTCTCCATGC
    ATTGCAAGAA GCACAGGCAC ACCATCCCCT CTTTCCCCAA TGCCTTCTCC TGCCAGCTCC
    GTAGGGTCCC AGTCAAGTGC TGGCAGTGTG GGGAGCAGTG GGGTGGCTGC CACTATCAGC
    ACCCCAGTCA CCATCCAGAA TATGACATCT TCCTATGTCA CCATCACATC CCATGTTCTT
    ACCGCCTTTG ACCTTTGGGA ACAGGCCGAG GCCCTCACGA GGAAGAATAA AGAATTCTTT
    GCTCGGCTCA GCACAAATGT GTGCACCTTG GCCCTCAACA GCAGTTTGGT GGACCTGGTG
    CACTATACAC GACAGGGTTT TCAGCAGCTA CAAGAATTAA CCAAAACACC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38942
    Clone ID Related Accession (Same CDS sequence) XM_005263011.3
    Accession Version XM_005263011.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263011.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCCCAGTA ATGCCATGTT TTTGATGCAC AGTTTGTACA ATGACGACAG AAACCTGCTT 
    CGAATTAGAG AGAAGGAAAG ACGCAACCAG GAAGCCCACC AAGAGAAAGA GGCATTTCCT
    GAAAAGATTC CCCTTTTTGG AGAGCCCTAC AAGACAGCAA AAGGTGATGA GCTGTCTAGT
    CGAATACAGA ACATGTTGGG AAACTACGAA GAAGTGAAGG AGTTCCTTAG TACTAAGTCT
    CACACTCATC GCCTGGATGC TTCTGAAAAT AGGTTGGGAA AGCCGAAATA TCCTTTAATT
    CCTGACAAAG GGAGCAGCAT TCCATCCAGC TCCTTCCACA CTAGTGTCCA CCACCAGTCC
    ATTCACACTC CTGCGTCTGG ACCACTTTCT GTTGGCAACA TTAGCCACAA TCCAAAGATG
    GCGCAGCCAA GAACTGAACC AATGCCAAGT CTCCATGCCA AAAGCTGCGG CCCACCGGAC
    AGCCAGCACC TGACCCAGGA TCGCCTTGGT CAGGAGGGGT TCGGCTCTAG TCATCACAAG
    AAAGGTGACC GAAGAGCTGA CGGAGACCAC TGTGCTTCGG TGACAGATTC GGCTCCAGAG
    AGGGAGCTTT CTCCCTTAAT CTCTTTGCCT TCCCCAGTTC CCCCTTTGTC ACCTATACAT
    TCCAACCAGC AAACTCTTCC CCGGACGCAA GGAAGCAGCA AGGTTCATGG CAGCAGCAAT
    AACAGTAAAG GCTATTGCCC AGCCAAATCT CCCAAGGACC TAGCAGTGAA AGTCCATGAT
    AAAGAGACCC CTCAAGACAG TTTGGTGGCC CCTGCCCAGC CGCCTTCTCA GACATTTCCA
    CCTCCCTCCC TCCCCTCAAA AAGTGTTGCA ATGCAGCAGA AGCCCACGGC TTATGTCCGG
    CCCATGGATG GTCAAGATCA GGCCCCTAGT GAATCCCCTG AACTGAAACC ACTGCCGGAG
    GACTATCGAC AGCAGACCTT TGAAAAAACA GACTTGAAAG TGCCTGCCAA AGCCAAGCTC
    ACCAAACTGA AGATGCCTTC TCAGTCAGTT GAGCAGACCT ACTCCAATGA AGTCCATTGT
    GTTGAAGAGA TTCTGAAGGA AATGACCCAT TCATGGCCGC CTCCTTTGAC AGCAATACAT
    ACGCCTAGTA CAGCTGAGCC ATCCAAGTTT CCTTTCCCTA CAAAGGACTC TCAGCATGTC
    AGTTCTGTAA CCCAAAACCA AAAACAATAT GATACATCTT CAAAAACTCA CTCAAATTCT
    CAGCAAGGAA CGTCATCCAT GCTCGAAGAC GACCTTCAGC TCAGTGACAG TGAGGACAGT
    GACAGTGAAC AAACCCCAGA GAAGCCTCCC TCCTCATCTG CACCTCCAAG TGCTCCACAG
    TCCCTTCCAG AACCAGTGGC ATCAGCACAT TCCAGCAGTG CAGAGTCAGA AAGCACCAGT
    GACTCAGACA GTTCCTCAGA CTCAGAGAGC GAGAGCAGTT CAAGTGACAG CGAAGAAAAT
    GAGCCCCTAG AAACCCCAGC TCCGGAGCCT GAGCCTCCAA CAACAAACAA ATGGCAGCTG
    GACAACTGGC TGACCAAAGT CAGCCAGCCA GCTGCGCCAC CAGAGGGCCC CAGGAGCACA
    GAGCCCCCAC GGCGGCACCC AGAGAGTAAG GGCAGCAGCG ACAGTGCCAC GAGTCAGGAG
    CATTCTGAAT CCAAAGATCC TCCCCCTAAA AGCTCCAGCA AAGCCCCCCG GGCCCCACCC
    GAAGCCCCCC ACCCCGGAAA GAGGAGCTGT CAGAAGTCTC CGGCACAGCA GGAGCCCCCA
    CAAAGGCAAA CCGTTGGAAC CAAACAACCC AAAAAACCTG TCAAGGCCTC TGCCCGGGCA
    GGTTCACGGA CCAGCCTGCA GGGGGAAAGG GAGCCAGGGC TTCTTCCCTA TGGCTCCCGA
    GACCAGACTT CCAAAGACAA GCCCAAGGTG AAGACGAAAG GACGGCCCCG GGCCGCAGCA
    AGCAACGAAC CCAAGCCAGC AGTGCCCCCC TCCAGTGAGA AGAAGAAGCA CAAGAGCTCC
    CTCCCTGCCC CCTCTAAGGC TCTCTCAGGC CCAGAACCCG CGAAGGACAA TGTGGAGGAC
    AGGACCCCTG AGCACTTTGC TCTTGTTCCC CTGACTGAGA GCCAGGGCCC ACCCCACAGT
    GGCAGCGGCA GCAGGACTAG TGGCTGCCGC CAAGCCGTGG TGGTCCAGGA GGACAGCCGC
    AAAGACAGAC TCCCATTGCC TTTGAGAGAC ACCAAGCTGC TCTCACCGCT CAGGGACACT
    CCTCCCCCAC AAAGCTTGAT GGTGAAGATC ACCCTAGACC TGCTCTCTCG GATACCCCAG
    CCTCCCGGGA AGGGGAGCCG CCAGAGGAAA GCAGAAGATA AACAGCCGCC CGCAGGGAAG
    AAGCACAGCT CTGAGAAGAG GAGCTCAGAC AGCTCAAGCA AGTTGGCCAA AAAGAGAAAG
    GGTGAAGCAG AAAGAGACTG TGATAACAAG AAAATCAGAC TGGAGAAGGA AATCAAATCA
    CAGTCATCTT CATCTTCATC CTCCCACAAA GAATCTTCTA AAACAAAGCC CTCCAGGCCC
    TCCTCACAGT CCTCAAAGAA GGAAATGCTC CCCCCGCCAC CCGTGTCCTC GTCCTCCCAG
    AAGCCAGCCA AGCCTGCACT TAAGAGGTCA AGGCGGGAAG CAGACACCTG TGGCCAGGAC
    CCTCCCAAAA GTGCCAGCAG TACCAAGAGC AACCACAAAG ACTCTTCCAT TCCCAAGCAG
    AGAAGAGTAG AGGGGAAGGG CTCCAGAAGC TCCTCGGAGC ACAAGGGTTC TTCCGGAGAT
    ACTGCAAATC CTTTTCCAGT GCCTTCTTTG CCAAATGGTA ACTCTAAACC AGGGAAGCCT
    CAAGTGAAGT TTGACAAACA ACAAGCAGAC CTTCACATGA GGGAGGCAAA AAAGATGAAG
    CAGAAAGCAG AGTTAATGAC GGACAGGGTT GGAAAGGCTT TTAAGTACCT GGAAGCCGTC
    TTGTCCTTCA TTGAGTGCGG AATTGCCACA GAGTCTGAAA GCCAGTCATC CAAGTCAGCT
    TACTCTGTCT ACTCAGAAAC TGTAGATCTC ATTAAATTCA TAATGTCATT AAAATCCTTC
    TCAGATGCCA CAGCGCCAAC ACAAGAGAAA ATATTTGCTG TTTTATGCAT GCGTTGCCAG
    TCCATTTTGA ACATGGCGAT GTTTCGTTGT AAAAAAGACA TAGCAATAAA GTATTCTCGT
    ACTCTTAATA AACACTTCGA GAGTTCTTCC AAAGTCGCCC AGGCACCTTC TCCATGCATT
    GCAAGAAGCA CAGGCACACC ATCCCCTCTT TCCCCAATGC CTTCTCCTGC CAGCTCCGTA
    GGGTCCCAGT CAAGTGCTGG CAGTGTGGGG AGCAGTGGGG TGGCTGCCAC TATCAGCACC
    CCAGTCACCA TCCAGAATAT GACATCTTCC TATGTCACCA TCACATCCCA TGTTCTTACC
    GCCTTTGACC TTTGGGAACA GGCCGAGGCC CTCACGAGGA AGAATAAAGA ATTCTTTGCT
    CGGCTCAGCA CAAATGTGTG CACCTTGGCC CTCAACAGCA GTTTGGTGGA CCTGGTGCAC
    TATACACGAC AGGGTTTTCA GCAGCTACAA GAATTAACCA AAACACCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263068.1
    CDS68..3718
    Translation

    Target ORF information:

    RefSeq Version XM_005263011.3
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263011.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCCCAGTA ATGCCATGTT TTTGATGCAC AGTTTGTACA ATGACGACAG AAACCTGCTT 
    CGAATTAGAG AGAAGGAAAG ACGCAACCAG GAAGCCCACC AAGAGAAAGA GGCATTTCCT
    GAAAAGATTC CCCTTTTTGG AGAGCCCTAC AAGACAGCAA AAGGTGATGA GCTGTCTAGT
    CGAATACAGA ACATGTTGGG AAACTACGAA GAAGTGAAGG AGTTCCTTAG TACTAAGTCT
    CACACTCATC GCCTGGATGC TTCTGAAAAT AGGTTGGGAA AGCCGAAATA TCCTTTAATT
    CCTGACAAAG GGAGCAGCAT TCCATCCAGC TCCTTCCACA CTAGTGTCCA CCACCAGTCC
    ATTCACACTC CTGCGTCTGG ACCACTTTCT GTTGGCAACA TTAGCCACAA TCCAAAGATG
    GCGCAGCCAA GAACTGAACC AATGCCAAGT CTCCATGCCA AAAGCTGCGG CCCACCGGAC
    AGCCAGCACC TGACCCAGGA TCGCCTTGGT CAGGAGGGGT TCGGCTCTAG TCATCACAAG
    AAAGGTGACC GAAGAGCTGA CGGAGACCAC TGTGCTTCGG TGACAGATTC GGCTCCAGAG
    AGGGAGCTTT CTCCCTTAAT CTCTTTGCCT TCCCCAGTTC CCCCTTTGTC ACCTATACAT
    TCCAACCAGC AAACTCTTCC CCGGACGCAA GGAAGCAGCA AGGTTCATGG CAGCAGCAAT
    AACAGTAAAG GCTATTGCCC AGCCAAATCT CCCAAGGACC TAGCAGTGAA AGTCCATGAT
    AAAGAGACCC CTCAAGACAG TTTGGTGGCC CCTGCCCAGC CGCCTTCTCA GACATTTCCA
    CCTCCCTCCC TCCCCTCAAA AAGTGTTGCA ATGCAGCAGA AGCCCACGGC TTATGTCCGG
    CCCATGGATG GTCAAGATCA GGCCCCTAGT GAATCCCCTG AACTGAAACC ACTGCCGGAG
    GACTATCGAC AGCAGACCTT TGAAAAAACA GACTTGAAAG TGCCTGCCAA AGCCAAGCTC
    ACCAAACTGA AGATGCCTTC TCAGTCAGTT GAGCAGACCT ACTCCAATGA AGTCCATTGT
    GTTGAAGAGA TTCTGAAGGA AATGACCCAT TCATGGCCGC CTCCTTTGAC AGCAATACAT
    ACGCCTAGTA CAGCTGAGCC ATCCAAGTTT CCTTTCCCTA CAAAGGACTC TCAGCATGTC
    AGTTCTGTAA CCCAAAACCA AAAACAATAT GATACATCTT CAAAAACTCA CTCAAATTCT
    CAGCAAGGAA CGTCATCCAT GCTCGAAGAC GACCTTCAGC TCAGTGACAG TGAGGACAGT
    GACAGTGAAC AAACCCCAGA GAAGCCTCCC TCCTCATCTG CACCTCCAAG TGCTCCACAG
    TCCCTTCCAG AACCAGTGGC ATCAGCACAT TCCAGCAGTG CAGAGTCAGA AAGCACCAGT
    GACTCAGACA GTTCCTCAGA CTCAGAGAGC GAGAGCAGTT CAAGTGACAG CGAAGAAAAT
    GAGCCCCTAG AAACCCCAGC TCCGGAGCCT GAGCCTCCAA CAACAAACAA ATGGCAGCTG
    GACAACTGGC TGACCAAAGT CAGCCAGCCA GCTGCGCCAC CAGAGGGCCC CAGGAGCACA
    GAGCCCCCAC GGCGGCACCC AGAGAGTAAG GGCAGCAGCG ACAGTGCCAC GAGTCAGGAG
    CATTCTGAAT CCAAAGATCC TCCCCCTAAA AGCTCCAGCA AAGCCCCCCG GGCCCCACCC
    GAAGCCCCCC ACCCCGGAAA GAGGAGCTGT CAGAAGTCTC CGGCACAGCA GGAGCCCCCA
    CAAAGGCAAA CCGTTGGAAC CAAACAACCC AAAAAACCTG TCAAGGCCTC TGCCCGGGCA
    GGTTCACGGA CCAGCCTGCA GGGGGAAAGG GAGCCAGGGC TTCTTCCCTA TGGCTCCCGA
    GACCAGACTT CCAAAGACAA GCCCAAGGTG AAGACGAAAG GACGGCCCCG GGCCGCAGCA
    AGCAACGAAC CCAAGCCAGC AGTGCCCCCC TCCAGTGAGA AGAAGAAGCA CAAGAGCTCC
    CTCCCTGCCC CCTCTAAGGC TCTCTCAGGC CCAGAACCCG CGAAGGACAA TGTGGAGGAC
    AGGACCCCTG AGCACTTTGC TCTTGTTCCC CTGACTGAGA GCCAGGGCCC ACCCCACAGT
    GGCAGCGGCA GCAGGACTAG TGGCTGCCGC CAAGCCGTGG TGGTCCAGGA GGACAGCCGC
    AAAGACAGAC TCCCATTGCC TTTGAGAGAC ACCAAGCTGC TCTCACCGCT CAGGGACACT
    CCTCCCCCAC AAAGCTTGAT GGTGAAGATC ACCCTAGACC TGCTCTCTCG GATACCCCAG
    CCTCCCGGGA AGGGGAGCCG CCAGAGGAAA GCAGAAGATA AACAGCCGCC CGCAGGGAAG
    AAGCACAGCT CTGAGAAGAG GAGCTCAGAC AGCTCAAGCA AGTTGGCCAA AAAGAGAAAG
    GGTGAAGCAG AAAGAGACTG TGATAACAAG AAAATCAGAC TGGAGAAGGA AATCAAATCA
    CAGTCATCTT CATCTTCATC CTCCCACAAA GAATCTTCTA AAACAAAGCC CTCCAGGCCC
    TCCTCACAGT CCTCAAAGAA GGAAATGCTC CCCCCGCCAC CCGTGTCCTC GTCCTCCCAG
    AAGCCAGCCA AGCCTGCACT TAAGAGGTCA AGGCGGGAAG CAGACACCTG TGGCCAGGAC
    CCTCCCAAAA GTGCCAGCAG TACCAAGAGC AACCACAAAG ACTCTTCCAT TCCCAAGCAG
    AGAAGAGTAG AGGGGAAGGG CTCCAGAAGC TCCTCGGAGC ACAAGGGTTC TTCCGGAGAT
    ACTGCAAATC CTTTTCCAGT GCCTTCTTTG CCAAATGGTA ACTCTAAACC AGGGAAGCCT
    CAAGTGAAGT TTGACAAACA ACAAGCAGAC CTTCACATGA GGGAGGCAAA AAAGATGAAG
    CAGAAAGCAG AGTTAATGAC GGACAGGGTT GGAAAGGCTT TTAAGTACCT GGAAGCCGTC
    TTGTCCTTCA TTGAGTGCGG AATTGCCACA GAGTCTGAAA GCCAGTCATC CAAGTCAGCT
    TACTCTGTCT ACTCAGAAAC TGTAGATCTC ATTAAATTCA TAATGTCATT AAAATCCTTC
    TCAGATGCCA CAGCGCCAAC ACAAGAGAAA ATATTTGCTG TTTTATGCAT GCGTTGCCAG
    TCCATTTTGA ACATGGCGAT GTTTCGTTGT AAAAAAGACA TAGCAATAAA GTATTCTCGT
    ACTCTTAATA AACACTTCGA GAGTTCTTCC AAAGTCGCCC AGGCACCTTC TCCATGCATT
    GCAAGAAGCA CAGGCACACC ATCCCCTCTT TCCCCAATGC CTTCTCCTGC CAGCTCCGTA
    GGGTCCCAGT CAAGTGCTGG CAGTGTGGGG AGCAGTGGGG TGGCTGCCAC TATCAGCACC
    CCAGTCACCA TCCAGAATAT GACATCTTCC TATGTCACCA TCACATCCCA TGTTCTTACC
    GCCTTTGACC TTTGGGAACA GGCCGAGGCC CTCACGAGGA AGAATAAAGA ATTCTTTGCT
    CGGCTCAGCA CAAATGTGTG CACCTTGGCC CTCAACAGCA GTTTGGTGGA CCTGGTGCAC
    TATACACGAC AGGGTTTTCA GCAGCTACAA GAATTAACCA AAACACCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38944
    Clone ID Related Accession (Same CDS sequence) XM_005263013.3 , XM_005263013.4
    Accession Version XM_005263013.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263013.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCATTTA CAGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA
    TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG
    TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT
    AAGTCTCACA CTCATCGCCT GGATGCTTCT GAAAATAGGT TGGGAAAGCC GAAATATCCT
    TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC
    CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA
    AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGCGGCCCA
    CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT
    CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGATTCGGCT
    CCAGAGAGGG AGCTTTCTCC CTTAATCTCT TTGCCTTCCC CAGTTCCCCC TTTGTCACCT
    ATACATTCCA ACCAGCAAAC TCTTCCCCGG ACGCAAGGAA GCAGCAAGGT TCATGGCAGC
    AGCAATAACA GTAAAGGCTA TTGCCCAGCC AAATCTCCCA AGGACCTAGC AGTGAAAGTC
    CATGATAAAG AGACCCCTCA AGACAGTTTG GTGGCCCCTG CCCAGCCGCC TTCTCAGACA
    TTTCCACCTC CCTCCCTCCC CTCAAAAAGT GTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGATGGTCA AGATCAGGCC CCTAGTGAAT CCCCTGAACT GAAACCACTG
    CCGGAGGACT ATCGACAGCA GACCTTTGAA AAAACAGACT TGAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCTTCTCAG TCAGTTGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCATTCAT GGCCGCCTCC TTTGACAGCA
    ATACATACGC CTAGTACAGC TGAGCCATCC AAGTTTCCTT TCCCTACAAA GGACTCTCAG
    CATGTCAGTT CTGTAACCCA AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACTCA
    AATTCTCAGC AAGGAACGTC ATCCATGCTC GAAGACGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GTGAACAAAC CCCAGAGAAG CCTCCCTCCT CATCTGCACC TCCAAGTGCT
    CCACAGTCCC TTCCAGAACC AGTGGCATCA GCACATTCCA GCAGTGCAGA GTCAGAAAGC
    ACCAGTGACT CAGACAGTTC CTCAGACTCA GAGAGCGAGA GCAGTTCAAG TGACAGCGAA
    GAAAATGAGC CCCTAGAAAC CCCAGCTCCG GAGCCTGAGC CTCCAACAAC AAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCAGCTG CGCCACCAGA GGGCCCCAGG
    AGCACAGAGC CCCCACGGCG GCACCCAGAG AGTAAGGGCA GCAGCGACAG TGCCACGAGT
    CAGGAGCATT CTGAATCCAA AGATCCTCCC CCTAAAAGCT CCAGCAAAGC CCCCCGGGCC
    CCACCCGAAG CCCCCCACCC CGGAAAGAGG AGCTGTCAGA AGTCTCCGGC ACAGCAGGAG
    CCCCCACAAA GGCAAACCGT TGGAACCAAA CAACCCAAAA AACCTGTCAA GGCCTCTGCC
    CGGGCAGGTT CACGGACCAG CCTGCAGGGG GAAAGGGAGC CAGGGCTTCT TCCCTATGGC
    TCCCGAGACC AGACTTCCAA AGACAAGCCC AAGGTGAAGA CGAAAGGACG GCCCCGGGCC
    GCAGCAAGCA ACGAACCCAA GCCAGCAGTG CCCCCCTCCA GTGAGAAGAA GAAGCACAAG
    AGCTCCCTCC CTGCCCCCTC TAAGGCTCTC TCAGGCCCAG AACCCGCGAA GGACAATGTG
    GAGGACAGGA CCCCTGAGCA CTTTGCTCTT GTTCCCCTGA CTGAGAGCCA GGGCCCACCC
    CACAGTGGCA GCGGCAGCAG GACTAGTGGC TGCCGCCAAG CCGTGGTGGT CCAGGAGGAC
    AGCCGCAAAG ACAGACTCCC ATTGCCTTTG AGAGACACCA AGCTGCTCTC ACCGCTCAGG
    GACACTCCTC CCCCACAAAG CTTGATGGTG AAGATCACCC TAGACCTGCT CTCTCGGATA
    CCCCAGCCTC CCGGGAAGGG GAGCCGCCAG AGGAAAGCAG AAGATAAACA GCCGCCCGCA
    GGGAAGAAGC ACAGCTCTGA GAAGAGGAGC TCAGACAGCT CAAGCAAGTT GGCCAAAAAG
    AGAAAGGGTG AAGCAGAAAG AGACTGTGAT AACAAGAAAA TCAGACTGGA GAAGGAAATC
    AAATCACAGT CATCTTCATC TTCATCCTCC CACAAAGAAT CTTCTAAAAC AAAGCCCTCC
    AGGCCCTCCT CACAGTCCTC AAAGAAGGAA ATGCTCCCCC CGCCACCCGT GTCCTCGTCC
    TCCCAGAAGC CAGCCAAGCC TGCACTTAAG AGGTCAAGGC GGGAAGCAGA CACCTGTGGC
    CAGGACCCTC CCAAAAGTGC CAGCAGTACC AAGAGCAACC ACAAAGACTC TTCCATTCCC
    AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC AGAAGCTCCT CGGAGCACAA GGGTTCTTCC
    GGAGATACTG CAAATCCTTT TCCAGTGCCT TCTTTGCCAA ATGGTAACTC TAAACCAGGG
    AAGCCTCAAG TGAAGTTTGA CAAACAACAA GCAGACCTTC ACATGAGGGA GGCAAAAAAG
    ATGAAGCAGA AAGCAGAGTT AATGACGGAC AGGGTTGGAA AGGCTTTTAA GTACCTGGAA
    GCCGTCTTGT CCTTCATTGA GTGCGGAATT GCCACAGAGT CTGAAAGCCA GTCATCCAAG
    TCAGCTTACT CTGTCTACTC AGAAACTGTA GATCTCATTA AATTCATAAT GTCATTAAAA
    TCCTTCTCAG ATGCCACAGC GCCAACACAA GAGAAAATAT TTGCTGTTTT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263070.1
    CDS271..3444
    Translation

    Target ORF information:

    RefSeq Version XM_005263013.3
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263013.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCATTTA CAGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA
    TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG
    TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT
    AAGTCTCACA CTCATCGCCT GGATGCTTCT GAAAATAGGT TGGGAAAGCC GAAATATCCT
    TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC
    CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA
    AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGCGGCCCA
    CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT
    CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGATTCGGCT
    CCAGAGAGGG AGCTTTCTCC CTTAATCTCT TTGCCTTCCC CAGTTCCCCC TTTGTCACCT
    ATACATTCCA ACCAGCAAAC TCTTCCCCGG ACGCAAGGAA GCAGCAAGGT TCATGGCAGC
    AGCAATAACA GTAAAGGCTA TTGCCCAGCC AAATCTCCCA AGGACCTAGC AGTGAAAGTC
    CATGATAAAG AGACCCCTCA AGACAGTTTG GTGGCCCCTG CCCAGCCGCC TTCTCAGACA
    TTTCCACCTC CCTCCCTCCC CTCAAAAAGT GTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGATGGTCA AGATCAGGCC CCTAGTGAAT CCCCTGAACT GAAACCACTG
    CCGGAGGACT ATCGACAGCA GACCTTTGAA AAAACAGACT TGAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCTTCTCAG TCAGTTGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCATTCAT GGCCGCCTCC TTTGACAGCA
    ATACATACGC CTAGTACAGC TGAGCCATCC AAGTTTCCTT TCCCTACAAA GGACTCTCAG
    CATGTCAGTT CTGTAACCCA AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACTCA
    AATTCTCAGC AAGGAACGTC ATCCATGCTC GAAGACGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GTGAACAAAC CCCAGAGAAG CCTCCCTCCT CATCTGCACC TCCAAGTGCT
    CCACAGTCCC TTCCAGAACC AGTGGCATCA GCACATTCCA GCAGTGCAGA GTCAGAAAGC
    ACCAGTGACT CAGACAGTTC CTCAGACTCA GAGAGCGAGA GCAGTTCAAG TGACAGCGAA
    GAAAATGAGC CCCTAGAAAC CCCAGCTCCG GAGCCTGAGC CTCCAACAAC AAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCAGCTG CGCCACCAGA GGGCCCCAGG
    AGCACAGAGC CCCCACGGCG GCACCCAGAG AGTAAGGGCA GCAGCGACAG TGCCACGAGT
    CAGGAGCATT CTGAATCCAA AGATCCTCCC CCTAAAAGCT CCAGCAAAGC CCCCCGGGCC
    CCACCCGAAG CCCCCCACCC CGGAAAGAGG AGCTGTCAGA AGTCTCCGGC ACAGCAGGAG
    CCCCCACAAA GGCAAACCGT TGGAACCAAA CAACCCAAAA AACCTGTCAA GGCCTCTGCC
    CGGGCAGGTT CACGGACCAG CCTGCAGGGG GAAAGGGAGC CAGGGCTTCT TCCCTATGGC
    TCCCGAGACC AGACTTCCAA AGACAAGCCC AAGGTGAAGA CGAAAGGACG GCCCCGGGCC
    GCAGCAAGCA ACGAACCCAA GCCAGCAGTG CCCCCCTCCA GTGAGAAGAA GAAGCACAAG
    AGCTCCCTCC CTGCCCCCTC TAAGGCTCTC TCAGGCCCAG AACCCGCGAA GGACAATGTG
    GAGGACAGGA CCCCTGAGCA CTTTGCTCTT GTTCCCCTGA CTGAGAGCCA GGGCCCACCC
    CACAGTGGCA GCGGCAGCAG GACTAGTGGC TGCCGCCAAG CCGTGGTGGT CCAGGAGGAC
    AGCCGCAAAG ACAGACTCCC ATTGCCTTTG AGAGACACCA AGCTGCTCTC ACCGCTCAGG
    GACACTCCTC CCCCACAAAG CTTGATGGTG AAGATCACCC TAGACCTGCT CTCTCGGATA
    CCCCAGCCTC CCGGGAAGGG GAGCCGCCAG AGGAAAGCAG AAGATAAACA GCCGCCCGCA
    GGGAAGAAGC ACAGCTCTGA GAAGAGGAGC TCAGACAGCT CAAGCAAGTT GGCCAAAAAG
    AGAAAGGGTG AAGCAGAAAG AGACTGTGAT AACAAGAAAA TCAGACTGGA GAAGGAAATC
    AAATCACAGT CATCTTCATC TTCATCCTCC CACAAAGAAT CTTCTAAAAC AAAGCCCTCC
    AGGCCCTCCT CACAGTCCTC AAAGAAGGAA ATGCTCCCCC CGCCACCCGT GTCCTCGTCC
    TCCCAGAAGC CAGCCAAGCC TGCACTTAAG AGGTCAAGGC GGGAAGCAGA CACCTGTGGC
    CAGGACCCTC CCAAAAGTGC CAGCAGTACC AAGAGCAACC ACAAAGACTC TTCCATTCCC
    AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC AGAAGCTCCT CGGAGCACAA GGGTTCTTCC
    GGAGATACTG CAAATCCTTT TCCAGTGCCT TCTTTGCCAA ATGGTAACTC TAAACCAGGG
    AAGCCTCAAG TGAAGTTTGA CAAACAACAA GCAGACCTTC ACATGAGGGA GGCAAAAAAG
    ATGAAGCAGA AAGCAGAGTT AATGACGGAC AGGGTTGGAA AGGCTTTTAA GTACCTGGAA
    GCCGTCTTGT CCTTCATTGA GTGCGGAATT GCCACAGAGT CTGAAAGCCA GTCATCCAAG
    TCAGCTTACT CTGTCTACTC AGAAACTGTA GATCTCATTA AATTCATAAT GTCATTAAAA
    TCCTTCTCAG ATGCCACAGC GCCAACACAA GAGAAAATAT TTGCTGTTTT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38944
    Clone ID Related Accession (Same CDS sequence) XM_005263013.3 , XM_005263013.4
    Accession Version XM_005263013.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263013.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCATTTA CAGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA
    TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG
    TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT
    AAGTCTCACA CTCATCGCCT GGATGCTTCT GAAAATAGGT TGGGAAAGCC GAAATATCCT
    TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC
    CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA
    AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGCGGCCCA
    CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT
    CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGATTCGGCT
    CCAGAGAGGG AGCTTTCTCC CTTAATCTCT TTGCCTTCCC CAGTTCCCCC TTTGTCACCT
    ATACATTCCA ACCAGCAAAC TCTTCCCCGG ACGCAAGGAA GCAGCAAGGT TCATGGCAGC
    AGCAATAACA GTAAAGGCTA TTGCCCAGCC AAATCTCCCA AGGACCTAGC AGTGAAAGTC
    CATGATAAAG AGACCCCTCA AGACAGTTTG GTGGCCCCTG CCCAGCCGCC TTCTCAGACA
    TTTCCACCTC CCTCCCTCCC CTCAAAAAGT GTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGATGGTCA AGATCAGGCC CCTAGTGAAT CCCCTGAACT GAAACCACTG
    CCGGAGGACT ATCGACAGCA GACCTTTGAA AAAACAGACT TGAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCTTCTCAG TCAGTTGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCATTCAT GGCCGCCTCC TTTGACAGCA
    ATACATACGC CTAGTACAGC TGAGCCATCC AAGTTTCCTT TCCCTACAAA GGACTCTCAG
    CATGTCAGTT CTGTAACCCA AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACTCA
    AATTCTCAGC AAGGAACGTC ATCCATGCTC GAAGACGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GTGAACAAAC CCCAGAGAAG CCTCCCTCCT CATCTGCACC TCCAAGTGCT
    CCACAGTCCC TTCCAGAACC AGTGGCATCA GCACATTCCA GCAGTGCAGA GTCAGAAAGC
    ACCAGTGACT CAGACAGTTC CTCAGACTCA GAGAGCGAGA GCAGTTCAAG TGACAGCGAA
    GAAAATGAGC CCCTAGAAAC CCCAGCTCCG GAGCCTGAGC CTCCAACAAC AAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCAGCTG CGCCACCAGA GGGCCCCAGG
    AGCACAGAGC CCCCACGGCG GCACCCAGAG AGTAAGGGCA GCAGCGACAG TGCCACGAGT
    CAGGAGCATT CTGAATCCAA AGATCCTCCC CCTAAAAGCT CCAGCAAAGC CCCCCGGGCC
    CCACCCGAAG CCCCCCACCC CGGAAAGAGG AGCTGTCAGA AGTCTCCGGC ACAGCAGGAG
    CCCCCACAAA GGCAAACCGT TGGAACCAAA CAACCCAAAA AACCTGTCAA GGCCTCTGCC
    CGGGCAGGTT CACGGACCAG CCTGCAGGGG GAAAGGGAGC CAGGGCTTCT TCCCTATGGC
    TCCCGAGACC AGACTTCCAA AGACAAGCCC AAGGTGAAGA CGAAAGGACG GCCCCGGGCC
    GCAGCAAGCA ACGAACCCAA GCCAGCAGTG CCCCCCTCCA GTGAGAAGAA GAAGCACAAG
    AGCTCCCTCC CTGCCCCCTC TAAGGCTCTC TCAGGCCCAG AACCCGCGAA GGACAATGTG
    GAGGACAGGA CCCCTGAGCA CTTTGCTCTT GTTCCCCTGA CTGAGAGCCA GGGCCCACCC
    CACAGTGGCA GCGGCAGCAG GACTAGTGGC TGCCGCCAAG CCGTGGTGGT CCAGGAGGAC
    AGCCGCAAAG ACAGACTCCC ATTGCCTTTG AGAGACACCA AGCTGCTCTC ACCGCTCAGG
    GACACTCCTC CCCCACAAAG CTTGATGGTG AAGATCACCC TAGACCTGCT CTCTCGGATA
    CCCCAGCCTC CCGGGAAGGG GAGCCGCCAG AGGAAAGCAG AAGATAAACA GCCGCCCGCA
    GGGAAGAAGC ACAGCTCTGA GAAGAGGAGC TCAGACAGCT CAAGCAAGTT GGCCAAAAAG
    AGAAAGGGTG AAGCAGAAAG AGACTGTGAT AACAAGAAAA TCAGACTGGA GAAGGAAATC
    AAATCACAGT CATCTTCATC TTCATCCTCC CACAAAGAAT CTTCTAAAAC AAAGCCCTCC
    AGGCCCTCCT CACAGTCCTC AAAGAAGGAA ATGCTCCCCC CGCCACCCGT GTCCTCGTCC
    TCCCAGAAGC CAGCCAAGCC TGCACTTAAG AGGTCAAGGC GGGAAGCAGA CACCTGTGGC
    CAGGACCCTC CCAAAAGTGC CAGCAGTACC AAGAGCAACC ACAAAGACTC TTCCATTCCC
    AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC AGAAGCTCCT CGGAGCACAA GGGTTCTTCC
    GGAGATACTG CAAATCCTTT TCCAGTGCCT TCTTTGCCAA ATGGTAACTC TAAACCAGGG
    AAGCCTCAAG TGAAGTTTGA CAAACAACAA GCAGACCTTC ACATGAGGGA GGCAAAAAAG
    ATGAAGCAGA AAGCAGAGTT AATGACGGAC AGGGTTGGAA AGGCTTTTAA GTACCTGGAA
    GCCGTCTTGT CCTTCATTGA GTGCGGAATT GCCACAGAGT CTGAAAGCCA GTCATCCAAG
    TCAGCTTACT CTGTCTACTC AGAAACTGTA GATCTCATTA AATTCATAAT GTCATTAAAA
    TCCTTCTCAG ATGCCACAGC GCCAACACAA GAGAAAATAT TTGCTGTTTT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263070.1
    CDS672..3845
    Translation

    Target ORF information:

    RefSeq Version XM_005263013.4
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 1 (AFF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263013.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCATTTA CAGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA
    TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG
    TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT
    AAGTCTCACA CTCATCGCCT GGATGCTTCT GAAAATAGGT TGGGAAAGCC GAAATATCCT
    TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC
    CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA
    AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGCGGCCCA
    CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT
    CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGATTCGGCT
    CCAGAGAGGG AGCTTTCTCC CTTAATCTCT TTGCCTTCCC CAGTTCCCCC TTTGTCACCT
    ATACATTCCA ACCAGCAAAC TCTTCCCCGG ACGCAAGGAA GCAGCAAGGT TCATGGCAGC
    AGCAATAACA GTAAAGGCTA TTGCCCAGCC AAATCTCCCA AGGACCTAGC AGTGAAAGTC
    CATGATAAAG AGACCCCTCA AGACAGTTTG GTGGCCCCTG CCCAGCCGCC TTCTCAGACA
    TTTCCACCTC CCTCCCTCCC CTCAAAAAGT GTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGATGGTCA AGATCAGGCC CCTAGTGAAT CCCCTGAACT GAAACCACTG
    CCGGAGGACT ATCGACAGCA GACCTTTGAA AAAACAGACT TGAAAGTGCC TGCCAAAGCC
    AAGCTCACCA AACTGAAGAT GCCTTCTCAG TCAGTTGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCATTCAT GGCCGCCTCC TTTGACAGCA
    ATACATACGC CTAGTACAGC TGAGCCATCC AAGTTTCCTT TCCCTACAAA GGACTCTCAG
    CATGTCAGTT CTGTAACCCA AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACTCA
    AATTCTCAGC AAGGAACGTC ATCCATGCTC GAAGACGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GTGAACAAAC CCCAGAGAAG CCTCCCTCCT CATCTGCACC TCCAAGTGCT
    CCACAGTCCC TTCCAGAACC AGTGGCATCA GCACATTCCA GCAGTGCAGA GTCAGAAAGC
    ACCAGTGACT CAGACAGTTC CTCAGACTCA GAGAGCGAGA GCAGTTCAAG TGACAGCGAA
    GAAAATGAGC CCCTAGAAAC CCCAGCTCCG GAGCCTGAGC CTCCAACAAC AAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCAGCTG CGCCACCAGA GGGCCCCAGG
    AGCACAGAGC CCCCACGGCG GCACCCAGAG AGTAAGGGCA GCAGCGACAG TGCCACGAGT
    CAGGAGCATT CTGAATCCAA AGATCCTCCC CCTAAAAGCT CCAGCAAAGC CCCCCGGGCC
    CCACCCGAAG CCCCCCACCC CGGAAAGAGG AGCTGTCAGA AGTCTCCGGC ACAGCAGGAG
    CCCCCACAAA GGCAAACCGT TGGAACCAAA CAACCCAAAA AACCTGTCAA GGCCTCTGCC
    CGGGCAGGTT CACGGACCAG CCTGCAGGGG GAAAGGGAGC CAGGGCTTCT TCCCTATGGC
    TCCCGAGACC AGACTTCCAA AGACAAGCCC AAGGTGAAGA CGAAAGGACG GCCCCGGGCC
    GCAGCAAGCA ACGAACCCAA GCCAGCAGTG CCCCCCTCCA GTGAGAAGAA GAAGCACAAG
    AGCTCCCTCC CTGCCCCCTC TAAGGCTCTC TCAGGCCCAG AACCCGCGAA GGACAATGTG
    GAGGACAGGA CCCCTGAGCA CTTTGCTCTT GTTCCCCTGA CTGAGAGCCA GGGCCCACCC
    CACAGTGGCA GCGGCAGCAG GACTAGTGGC TGCCGCCAAG CCGTGGTGGT CCAGGAGGAC
    AGCCGCAAAG ACAGACTCCC ATTGCCTTTG AGAGACACCA AGCTGCTCTC ACCGCTCAGG
    GACACTCCTC CCCCACAAAG CTTGATGGTG AAGATCACCC TAGACCTGCT CTCTCGGATA
    CCCCAGCCTC CCGGGAAGGG GAGCCGCCAG AGGAAAGCAG AAGATAAACA GCCGCCCGCA
    GGGAAGAAGC ACAGCTCTGA GAAGAGGAGC TCAGACAGCT CAAGCAAGTT GGCCAAAAAG
    AGAAAGGGTG AAGCAGAAAG AGACTGTGAT AACAAGAAAA TCAGACTGGA GAAGGAAATC
    AAATCACAGT CATCTTCATC TTCATCCTCC CACAAAGAAT CTTCTAAAAC AAAGCCCTCC
    AGGCCCTCCT CACAGTCCTC AAAGAAGGAA ATGCTCCCCC CGCCACCCGT GTCCTCGTCC
    TCCCAGAAGC CAGCCAAGCC TGCACTTAAG AGGTCAAGGC GGGAAGCAGA CACCTGTGGC
    CAGGACCCTC CCAAAAGTGC CAGCAGTACC AAGAGCAACC ACAAAGACTC TTCCATTCCC
    AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC AGAAGCTCCT CGGAGCACAA GGGTTCTTCC
    GGAGATACTG CAAATCCTTT TCCAGTGCCT TCTTTGCCAA ATGGTAACTC TAAACCAGGG
    AAGCCTCAAG TGAAGTTTGA CAAACAACAA GCAGACCTTC ACATGAGGGA GGCAAAAAAG
    ATGAAGCAGA AAGCAGAGTT AATGACGGAC AGGGTTGGAA AGGCTTTTAA GTACCTGGAA
    GCCGTCTTGT CCTTCATTGA GTGCGGAATT GCCACAGAGT CTGAAAGCCA GTCATCCAAG
    TCAGCTTACT CTGTCTACTC AGAAACTGTA GATCTCATTA AATTCATAAT GTCATTAAAA
    TCCTTCTCAG ATGCCACAGC GCCAACACAA GAGAAAATAT TTGCTGTTTT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.