AFF1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	AFF1
Entrez Gene ID	422590
Full Name	AF4/FMR2 family member 1
General protein information	Preferred Names AF4/FMR2 family member 1 Names AF4/FMR2 family member 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004941098.2	XP_004941155.1	AF4/FMR2 family member 1 isoform X1
XM_004941099.2	XP_004941156.1	AF4/FMR2 family member 1 isoform X3
XM_420549.5	XP_420549.4	AF4/FMR2 family member 1 isoform X2
XM_004941098.3	XP_004941155.1	AF4/FMR2 family member 1 isoform X1
XM_025150377.1	XP_025006145.1	AF4/FMR2 family member 1 isoform X3
XM_004941100.3	XP_004941157.1	AF4/FMR2 family member 1 isoform X4
XM_004941100.2	XP_004941157.1	AF4/FMR2 family member 1 isoform X4
XM_420549.6	XP_420549.4	AF4/FMR2 family member 1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AFF1	XP_001156948.2
Mus musculus (house mouse)	Aff1	NP_598680.3
Gallus gallus (chicken)	AFF1	XP_004941157.1
Homo sapiens (human)	AFF1	NP_001160165.1
	AFF1	XP_001095677.2
Canis lupus familiaris (dog)	AFF1	XP_005639216.1
Bos taurus (cattle)	AFF1	NP_001178454.1
Rattus norvegicus (Norway rat)	Aff1	NP_001100676.1

Gene Ontology

The following AFF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa08001	XM_004941098.3 Latest version!	Gallus gallus AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa08002	XM_420549.6 Latest version!	Gallus gallus AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa08003	XM_025150377.1 Latest version!	Gallus gallus AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa08003	XM_004941099.2 Latest version!	Gallus gallus AF4/FMR2 family, member 1 (AFF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa08004	XM_004941100.3 Latest version!	Gallus gallus AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OGa08001	XM_004941098.2	Gallus gallus AF4/FMR2 family, member 1 (AFF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa08004	XM_004941100.2	Gallus gallus AF4/FMR2 family, member 1 (AFF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa08002	XM_420549.5	Gallus gallus AF4/FMR2 family, member 1 (AFF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFF1 ( XM_004941098.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004941155.1 Gallus gallus AF4/FMR2 family, member 1 (AFF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa08001
Clone ID Related Accession (Same CDS sequence)	XM_004941098.2 , XM_004941098.3
Accession Version	XM_004941098.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	AF4/FMR2 family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004941098.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAA CGATGGCTGA GAGCTGCCAG AGGAAAGTTT GGCTGGGAGA ACGGCTGGTG 
CTAAAGGAGG TGGTGAAGAA ACCTGGTTTA GAAAGTCCAT TGCAGCCTGA GATGGGATTA 
CGTCCAAAAG GTGCCTCTGC TGCTTTCCCT GGGAGCTTGT ACAATGAGGA CAGAAACTTG 
CTGCGTCTTC AGGAGAGACA GAGGCGGAAT CAGGAGGCTC TGCAGGAGAA AGACAAGTTC 
CTTGAGAACA CCCCACTCTT TCCTGAGCCG TACAAGACTA ATAAAGAGGA TGAGTTGTCA 
AGCCGTATTC AGAATATGCT GGGCAATTAC GACGACTTGC AGGATCTTAT TAATGACAGA 
TGCCATCAGA ATCTCATAGG GATACCAGAG AGCGACGTTC CTCAGTTCCC ACAAAGAAAG 
CCCGACCGCC CATCCTTCCC TGAAAAGACC AGCCGTACGT TATCGTCTTC ATTCCACCAT 
GCAACCCGCT ATCCACACAT GGGACCCCCG GTCATTGCTC CTCCCCGTCC AGGCCACTAC 
GCTCCCCACC AAAAGGCACA ACCAGGAACA GGACCAGCTC CAGGCTGGCA TGCCAAGAGT 
CACAGCTCGT CCAGCAGCTG GAGCCAAGGT CAGGAACACA AGCGTGGCAG GCCGGACAGT 
CATGCCGGTT CTCACCACAA CAGAACTGAC AGGCATCCTG CTGGTGGAGG TCGTGCTCCT 
GAACCTCAGG CCACTCTTTT GGAGCTTTCC CCGTTGCTGC CGTCTTTGTC TTCTCCAGTG 
GCACCCCTGT CACCTCTACA TTCCATCCAG CACACTAATT CCAAGTCACA GAAGAGCAGC 
AAAGGTCATG GGCCGGCCTA CACTCAATCA CCTCAGGACC GAGCGGTGGG TTCACGGGAG 
AATAAAAGTC AGGACAACTC AGCCGTTATC TTGGCTGGCA CCACTCAGCC TTCCTCTCAA 
CCCTTTCCTC CACCTTTGCC ATCAAAAACC AGTGCAATGC AGCAGAAACC AACTGCCTAC 
GTCAGACCAA TGGATGGCCA AGAGCAGGCT CCGGTGGAGT CACCGGATCT CAAGCCTCTA 
CTGGAGGAAT ACCACGGTGA ACCGTATGAA AACATCTCAG ATTTGAAGGC AAACACCAAG 
GCCAAATTAT CCAAATTGAA CATCTCTTCC GAGCCCACTG AGCAAACCTT GCCCAGTGAT 
AGCCAGTGCG TTGAAGAAAT TTTGAAGGAA ATGACACACT CATGGCCACC TCTCTTGACA 
GCTATTCACA CGCCAAGTGC TGCAGAGCCC TCCAAGTTTC CCTTTCCAAC AAAGGAATCG 
CAGCATATCC CATCTGTAGC ACAGAATCAC AAGCAGTATG ACGCACCTTC CAAAACGTTG 
CCTAGTTCTC ATCCAACAAT ATCAGTGCTC CAGGAGGACC TGCAGCTCAG TGATAGTGAA 
GAGAGTGGTG ACGACCAAGT TGTTGAACAG CCACCTTCTT CACTTGATCC TCCAAGTGCC 
CTGCAGTCCC AGCCTGAGAG CGTGGCATCA GCACATTCCA ACAGCACAGA GTCAGGGAGC 
AGCAGTGACT CAGACAGCTC TTCAGACTCT TCAGAGACAG AGAGCAGCTC GAGTGACAGT 
GAAGCCAACG ACCCTCCAAG AGCGTCAGCT CCTGAGCCCG ACCCACCAAC TTCCAATAAG 
TGGCAGCTTG ACAACTGGCT CACCAAGGTG AACCCACCAG CAGTGCCAAC AGAAAGCCCC 
AGCGACACTG CCCACACAGA TGGCCACGAG GAGGACAAGG AACAACCCGT CAGCAACGCT 
TCCTCCTGCG AGCATGCTGT GCCAAGGGAA CCTCACGAGA GAAGCTCCAT CCGAGCAGCC 
CAGGCTCCTC AGGATGTTCA TCTTCCGACC AAACTTAGCT GCCAGAAGTC TCCTGTGCGC 
GCTGAGGAGC CCTCACAGAG GCAGACTGTG GGGATCAAAA GGCCCAGCAA GCCTCCAGTG 
CACGAGGAAC CCAAGGGAGG CCTGAAGGTG GAGAGCGAGC CCGCTCCTTA TGAGATTGGA 
GAGCAGTCTT CCAGAGACAA GCCAAAACCC AAAACAAAAA GCAGATCCAA ATCCTGTGAC 
CAAAAAGAAC TGAAACCCCG GCTGCAGGAG GCTCCTAAAG AGAAGAAGCA CAAGAGCTCC 
CACCACGCCG ATGCCAATGC CCTGCCGGAC CACAGACCCG TGAGTGATGT TCTGGTAGGC 
AGTGCCCAGG AGCCTTCCAC TCTCAAACAC CTCCCTGAGG GCCAGGGCAC AGCTCCCACC 
AGGACTAACG GCCACAGGTC TGCTGCTGAG GTCAAGGAGG GTTTTCACAA GGGGAAGCTT 
CCTGTGCCCA CCAAGGAGAA GAAGTTGCTG CCACCGGTGA GGGACGTGCC TGGCAATCCC 
AGCCTCCTGG TTAGAATTGA TCTCCCACTC CTGTCAAGAG TTCCCCAGCC TCCTGAGAAG 
GGGAACCAAA AGAAGAGAGC AGAGGTGAAG GAGCACCCTG GCACCAAGGA GCAGGACTTG 
GAGAAGAAAA GCACAGACAC TCCTGACAGG TCCTTCAAAA AGCGAAAGAG AGAAAAGGAG 
AAGGACGTCG ATAGGAAGAA AAGGAAGTTG GGCAAAGAGG CAAAATCATT GCAGTCTTCA 
CCTAACAAAG ACTCCTGTAA ATTGAAAGCA CGGAACGCTT CGCCTGAGAC CCAGAACAAA 
GATCTCAGGC AACCCCTGCC ACTGCCGCCC ATGTCTCCCA CGCATCCTGC ACCAAAGCCA 
ACCAAGAAGG CCCACAAGAG GCCCAGGAGT GAGAACGGCC AGCCGCCTGC CACTGCCAGC 
AGCACTGCCC TGGATGCAAG CAACCACAAG GATCCTTCTT CCACCAAGCA GAAGAAGATG 
GTGGAAGACC ACTCTGAGCA GGTGAAGGGC AACAAAGGAT CTGCAGGGGA TGTCACAAAT 
CCTTTACCAG TGCCTTCTGT GCCAAATGGT ACCTCCAAAC CAAGGAGACC TCAGATCAAG 
TTTGAAAGAC AGCATTTGTC AGAATATTAC CTGGAAGAGG CCAAGAAGAT GAAGCACGAG 
GCTGATGCCA TGATGGACAA GACTGGAAAG GCCTTTCAGT ACCTCGATGC TGCCCTTTCC 
TTTGTTGAAT ACGGGATGGC CCTGGAATCG AATGCGGTAG CACCAAAACC TGCTTACAGC 
ATCTTTGCTG ACACAATAGA TCTCCTCAAA TACGTAATGA CACTAAAACC CTTTACGGAT 
GTCTCGGCAT CTTCACATGA ACACGTCTTT GCTGTGTTAT GCATGCGCTG CCAGTCCCTC 
CTGCACATGG CAATGTTTCG TTACAAAAGA GACACTGCAA TAAAGTATTC CAGGATCCTG 
AATGACCACT TCAAGAGTTC TTCCAGAGTA ACACAGGCAC CTTCTCCATG TGTTGCAAGA 
AGCACAGGCA TGCCTTCTCC TCATTCTCCA GTGCCCTCTC CTGCCAGATC TATGAGTTCT 
CAGCAGGGAT CAAATGGTAG CAATTGTGGT TGCAACAGCA TTGGCTCTTC AGTCACCATC 
CCAAGCATCA CCTCTTCATA CGTCAACATC ACTTCCTATG TCCTCTATGC GTATAATATT 
TGGGAACATG CTGATGCACT GTCCAAGAAT AATAAGGAGT TTTTTGCTGA ACTCAGCACA 
GCGGTGTGCC CTCTGGCACT GACCAGTAGC ATGGCCGAAA TGGTACACTA CACTAGACAG 
GGTTTACATT GGCTGAGACT GAACACAAGT ACGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004941155.1
CDS	4..3762
Translation	MMKTMAESCQRKVWLGERLVLKEVVKKPGLESPLQPEMGLRPKGASAAFPGSLYNEDRNLLRLQERQRRNQEALQEKDKFLENTPLFPEPYKTNKEDELSSRIQNMLGNYDDLQDLINDRCHQNLIGIPESDVPQFPQRKPDRPSFPEKTSRTLSSSFHHATRYPHMGPPVIAPPRPGHYAPHQKAQPGTGPAPGWHAKSHSSSSSWSQGQEHKRGRPDSHAGSHHNRTDRHPAGGGRAPEPQATLLELSPLLPSLSSPVAPLSPLHSIQHTNSKSQKSSKGHGPAYTQSPQDRAVGSRENKSQDNSAVILAGTTQPSSQPFPPPLPSKTSAMQQKPTAYVRPMDGQEQAPVESPDLKPLLEEYHGEPYENISDLKANTKAKLSKLNISSEPTEQTLPSDSQCVEEILKEMTHSWPPLLTAIHTPSAAEPSKFPFPTKESQHIPSVAQNHKQYDAPSKTLPSSHPTISVLQEDLQLSDSEESGDDQVVEQPPSSLDPPSALQSQPESVASAHSNSTESGSSSDSDSSSDSSETESSSSDSEANDPPRASAPEPDPPTSNKWQLDNWLTKVNPPAVPTESPSDTAHTDGHEEDKEQPVSNASSCEHAVPREPHERSSIRAAQAPQDVHLPTKLSCQKSPVRAEEPSQRQTVGIKRPSKPPVHEEPKGGLKVESEPAPYEIGEQSSRDKPKPKTKSRSKSCDQKELKPRLQEAPKEKKHKSSHHADANALPDHRPVSDVLVGSAQEPSTLKHLPEGQGTAPTRTNGHRSAAEVKEGFHKGKLPVPTKEKKLLPPVRDVPGNPSLLVRIDLPLLSRVPQPPEKGNQKKRAEVKEHPGTKEQDLEKKSTDTPDRSFKKRKREKEKDVDRKKRKLGKEAKSLQSSPNKDSCKLKARNASPETQNKDLRQPLPLPPMSPTHPAPKPTKKAHKRPRSENGQPPATASSTALDASNHKDPSSTKQKKMVEDHSEQVKGNKGSAGDVTNPLPVPSVPNGTSKPRRPQIKFERQHLSEYYLEEAKKMKHEADAMMDKTGKAFQYLDAALSFVEYGMALESNAVAPKPAYSIFADTIDLLKYVMTLKPFTDVSASSHEHVFAVLCMRCQSLLHMAMFRYKRDTAIKYSRILNDHFKSSSRVTQAPSPCVARSTGMPSPHSPVPSPARSMSSQQGSNGSNCGCNSIGSSVTIPSITSSYVNITSYVLYAYNIWEHADALSKNNKEFFAELSTAVCPLALTSSMAEMVHYTRQGLHWLRLNTSTP

Target ORF information:

RefSeq Version	XM_004941098.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus AF4/FMR2 family, member 1 (AFF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004941098.2

ORF Insert Sequence:

ATGATGAAAA CGATGGCTGA GAGCTGCCAG AGGAAAGTTT GGCTGGGAGA ACGGCTGGTG 
CTAAAGGAGG TGGTGAAGAA ACCTGGTTTA GAAAGTCCAT TGCAGCCTGA GATGGGATTA 
CGTCCAAAAG GTGCCTCTGC TGCTTTCCCT GGGAGCTTGT ACAATGAGGA CAGAAACTTG 
CTGCGTCTTC AGGAGAGACA GAGGCGGAAT CAGGAGGCTC TGCAGGAGAA AGACAAGTTC 
CTTGAGAACA CCCCACTCTT TCCTGAGCCG TACAAGACTA ATAAAGAGGA TGAGTTGTCA 
AGCCGTATTC AGAATATGCT GGGCAATTAC GACGACTTGC AGGATCTTAT TAATGACAGA 
TGCCATCAGA ATCTCATAGG GATACCAGAG AGCGACGTTC CTCAGTTCCC ACAAAGAAAG 
CCCGACCGCC CATCCTTCCC TGAAAAGACC AGCCGTACGT TATCGTCTTC ATTCCACCAT 
GCAACCCGCT ATCCACACAT GGGACCCCCG GTCATTGCTC CTCCCCGTCC AGGCCACTAC 
GCTCCCCACC AAAAGGCACA ACCAGGAACA GGACCAGCTC CAGGCTGGCA TGCCAAGAGT 
CACAGCTCGT CCAGCAGCTG GAGCCAAGGT CAGGAACACA AGCGTGGCAG GCCGGACAGT 
CATGCCGGTT CTCACCACAA CAGAACTGAC AGGCATCCTG CTGGTGGAGG TCGTGCTCCT 
GAACCTCAGG CCACTCTTTT GGAGCTTTCC CCGTTGCTGC CGTCTTTGTC TTCTCCAGTG 
GCACCCCTGT CACCTCTACA TTCCATCCAG CACACTAATT CCAAGTCACA GAAGAGCAGC 
AAAGGTCATG GGCCGGCCTA CACTCAATCA CCTCAGGACC GAGCGGTGGG TTCACGGGAG 
AATAAAAGTC AGGACAACTC AGCCGTTATC TTGGCTGGCA CCACTCAGCC TTCCTCTCAA 
CCCTTTCCTC CACCTTTGCC ATCAAAAACC AGTGCAATGC AGCAGAAACC AACTGCCTAC 
GTCAGACCAA TGGATGGCCA AGAGCAGGCT CCGGTGGAGT CACCGGATCT CAAGCCTCTA 
CTGGAGGAAT ACCACGGTGA ACCGTATGAA AACATCTCAG ATTTGAAGGC AAACACCAAG 
GCCAAATTAT CCAAATTGAA CATCTCTTCC GAGCCCACTG AGCAAACCTT GCCCAGTGAT 
AGCCAGTGCG TTGAAGAAAT TTTGAAGGAA ATGACACACT CATGGCCACC TCTCTTGACA 
GCTATTCACA CGCCAAGTGC TGCAGAGCCC TCCAAGTTTC CCTTTCCAAC AAAGGAATCG 
CAGCATATCC CATCTGTAGC ACAGAATCAC AAGCAGTATG ACGCACCTTC CAAAACGTTG 
CCTAGTTCTC ATCCAACAAT ATCAGTGCTC CAGGAGGACC TGCAGCTCAG TGATAGTGAA 
GAGAGTGGTG ACGACCAAGT TGTTGAACAG CCACCTTCTT CACTTGATCC TCCAAGTGCC 
CTGCAGTCCC AGCCTGAGAG CGTGGCATCA GCACATTCCA ACAGCACAGA GTCAGGGAGC 
AGCAGTGACT CAGACAGCTC TTCAGACTCT TCAGAGACAG AGAGCAGCTC GAGTGACAGT 
GAAGCCAACG ACCCTCCAAG AGCGTCAGCT CCTGAGCCCG ACCCACCAAC TTCCAATAAG 
TGGCAGCTTG ACAACTGGCT CACCAAGGTG AACCCACCAG CAGTGCCAAC AGAAAGCCCC 
AGCGACACTG CCCACACAGA TGGCCACGAG GAGGACAAGG AACAACCCGT CAGCAACGCT 
TCCTCCTGCG AGCATGCTGT GCCAAGGGAA CCTCACGAGA GAAGCTCCAT CCGAGCAGCC 
CAGGCTCCTC AGGATGTTCA TCTTCCGACC AAACTTAGCT GCCAGAAGTC TCCTGTGCGC 
GCTGAGGAGC CCTCACAGAG GCAGACTGTG GGGATCAAAA GGCCCAGCAA GCCTCCAGTG 
CACGAGGAAC CCAAGGGAGG CCTGAAGGTG GAGAGCGAGC CCGCTCCTTA TGAGATTGGA 
GAGCAGTCTT CCAGAGACAA GCCAAAACCC AAAACAAAAA GCAGATCCAA ATCCTGTGAC 
CAAAAAGAAC TGAAACCCCG GCTGCAGGAG GCTCCTAAAG AGAAGAAGCA CAAGAGCTCC 
CACCACGCCG ATGCCAATGC CCTGCCGGAC CACAGACCCG TGAGTGATGT TCTGGTAGGC 
AGTGCCCAGG AGCCTTCCAC TCTCAAACAC CTCCCTGAGG GCCAGGGCAC AGCTCCCACC 
AGGACTAACG GCCACAGGTC TGCTGCTGAG GTCAAGGAGG GTTTTCACAA GGGGAAGCTT 
CCTGTGCCCA CCAAGGAGAA GAAGTTGCTG CCACCGGTGA GGGACGTGCC TGGCAATCCC 
AGCCTCCTGG TTAGAATTGA TCTCCCACTC CTGTCAAGAG TTCCCCAGCC TCCTGAGAAG 
GGGAACCAAA AGAAGAGAGC AGAGGTGAAG GAGCACCCTG GCACCAAGGA GCAGGACTTG 
GAGAAGAAAA GCACAGACAC TCCTGACAGG TCCTTCAAAA AGCGAAAGAG AGAAAAGGAG 
AAGGACGTCG ATAGGAAGAA AAGGAAGTTG GGCAAAGAGG CAAAATCATT GCAGTCTTCA 
CCTAACAAAG ACTCCTGTAA ATTGAAAGCA CGGAACGCTT CGCCTGAGAC CCAGAACAAA 
GATCTCAGGC AACCCCTGCC ACTGCCGCCC ATGTCTCCCA CGCATCCTGC ACCAAAGCCA 
ACCAAGAAGG CCCACAAGAG GCCCAGGAGT GAGAACGGCC AGCCGCCTGC CACTGCCAGC 
AGCACTGCCC TGGATGCAAG CAACCACAAG GATCCTTCTT CCACCAAGCA GAAGAAGATG 
GTGGAAGACC ACTCTGAGCA GGTGAAGGGC AACAAAGGAT CTGCAGGGGA TGTCACAAAT 
CCTTTACCAG TGCCTTCTGT GCCAAATGGT ACCTCCAAAC CAAGGAGACC TCAGATCAAG 
TTTGAAAGAC AGCATTTGTC AGAATATTAC CTGGAAGAGG CCAAGAAGAT GAAGCACGAG 
GCTGATGCCA TGATGGACAA GACTGGAAAG GCCTTTCAGT ACCTCGATGC TGCCCTTTCC 
TTTGTTGAAT ACGGGATGGC CCTGGAATCG AATGCGGTAG CACCAAAACC TGCTTACAGC 
ATCTTTGCTG ACACAATAGA TCTCCTCAAA TACGTAATGA CACTAAAACC CTTTACGGAT 
GTCTCGGCAT CTTCACATGA ACACGTCTTT GCTGTGTTAT GCATGCGCTG CCAGTCCCTC 
CTGCACATGG CAATGTTTCG TTACAAAAGA GACACTGCAA TAAAGTATTC CAGGATCCTG 
AATGACCACT TCAAGAGTTC TTCCAGAGTA ACACAGGCAC CTTCTCCATG TGTTGCAAGA 
AGCACAGGCA TGCCTTCTCC TCATTCTCCA GTGCCCTCTC CTGCCAGATC TATGAGTTCT 
CAGCAGGGAT CAAATGGTAG CAATTGTGGT TGCAACAGCA TTGGCTCTTC AGTCACCATC 
CCAAGCATCA CCTCTTCATA CGTCAACATC ACTTCCTATG TCCTCTATGC GTATAATATT 
TGGGAACATG CTGATGCACT GTCCAAGAAT AATAAGGAGT TTTTTGCTGA ACTCAGCACA 
GCGGTGTGCC CTCTGGCACT GACCAGTAGC ATGGCCGAAA TGGTACACTA CACTAGACAG 
GGTTTACATT GGCTGAGACT GAACACAAGT ACGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_004941098.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004941155.1 Gallus gallus AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa08001
Clone ID Related Accession (Same CDS sequence)	XM_004941098.2 , XM_004941098.3
Accession Version	XM_004941098.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	AF4/FMR2 family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004941098.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAA CGATGGCTGA GAGCTGCCAG AGGAAAGTTT GGCTGGGAGA ACGGCTGGTG 
CTAAAGGAGG TGGTGAAGAA ACCTGGTTTA GAAAGTCCAT TGCAGCCTGA GATGGGATTA 
CGTCCAAAAG GTGCCTCTGC TGCTTTCCCT GGGAGCTTGT ACAATGAGGA CAGAAACTTG 
CTGCGTCTTC AGGAGAGACA GAGGCGGAAT CAGGAGGCTC TGCAGGAGAA AGACAAGTTC 
CTTGAGAACA CCCCACTCTT TCCTGAGCCG TACAAGACTA ATAAAGAGGA TGAGTTGTCA 
AGCCGTATTC AGAATATGCT GGGCAATTAC GACGACTTGC AGGATCTTAT TAATGACAGA 
TGCCATCAGA ATCTCATAGG GATACCAGAG AGCGACGTTC CTCAGTTCCC ACAAAGAAAG 
CCCGACCGCC CATCCTTCCC TGAAAAGACC AGCCGTACGT TATCGTCTTC ATTCCACCAT 
GCAACCCGCT ATCCACACAT GGGACCCCCG GTCATTGCTC CTCCCCGTCC AGGCCACTAC 
GCTCCCCACC AAAAGGCACA ACCAGGAACA GGACCAGCTC CAGGCTGGCA TGCCAAGAGT 
CACAGCTCGT CCAGCAGCTG GAGCCAAGGT CAGGAACACA AGCGTGGCAG GCCGGACAGT 
CATGCCGGTT CTCACCACAA CAGAACTGAC AGGCATCCTG CTGGTGGAGG TCGTGCTCCT 
GAACCTCAGG CCACTCTTTT GGAGCTTTCC CCGTTGCTGC CGTCTTTGTC TTCTCCAGTG 
GCACCCCTGT CACCTCTACA TTCCATCCAG CACACTAATT CCAAGTCACA GAAGAGCAGC 
AAAGGTCATG GGCCGGCCTA CACTCAATCA CCTCAGGACC GAGCGGTGGG TTCACGGGAG 
AATAAAAGTC AGGACAACTC AGCCGTTATC TTGGCTGGCA CCACTCAGCC TTCCTCTCAA 
CCCTTTCCTC CACCTTTGCC ATCAAAAACC AGTGCAATGC AGCAGAAACC AACTGCCTAC 
GTCAGACCAA TGGATGGCCA AGAGCAGGCT CCGGTGGAGT CACCGGATCT CAAGCCTCTA 
CTGGAGGAAT ACCACGGTGA ACCGTATGAA AACATCTCAG ATTTGAAGGC AAACACCAAG 
GCCAAATTAT CCAAATTGAA CATCTCTTCC GAGCCCACTG AGCAAACCTT GCCCAGTGAT 
AGCCAGTGCG TTGAAGAAAT TTTGAAGGAA ATGACACACT CATGGCCACC TCTCTTGACA 
GCTATTCACA CGCCAAGTGC TGCAGAGCCC TCCAAGTTTC CCTTTCCAAC AAAGGAATCG 
CAGCATATCC CATCTGTAGC ACAGAATCAC AAGCAGTATG ACGCACCTTC CAAAACGTTG 
CCTAGTTCTC ATCCAACAAT ATCAGTGCTC CAGGAGGACC TGCAGCTCAG TGATAGTGAA 
GAGAGTGGTG ACGACCAAGT TGTTGAACAG CCACCTTCTT CACTTGATCC TCCAAGTGCC 
CTGCAGTCCC AGCCTGAGAG CGTGGCATCA GCACATTCCA ACAGCACAGA GTCAGGGAGC 
AGCAGTGACT CAGACAGCTC TTCAGACTCT TCAGAGACAG AGAGCAGCTC GAGTGACAGT 
GAAGCCAACG ACCCTCCAAG AGCGTCAGCT CCTGAGCCCG ACCCACCAAC TTCCAATAAG 
TGGCAGCTTG ACAACTGGCT CACCAAGGTG AACCCACCAG CAGTGCCAAC AGAAAGCCCC 
AGCGACACTG CCCACACAGA TGGCCACGAG GAGGACAAGG AACAACCCGT CAGCAACGCT 
TCCTCCTGCG AGCATGCTGT GCCAAGGGAA CCTCACGAGA GAAGCTCCAT CCGAGCAGCC 
CAGGCTCCTC AGGATGTTCA TCTTCCGACC AAACTTAGCT GCCAGAAGTC TCCTGTGCGC 
GCTGAGGAGC CCTCACAGAG GCAGACTGTG GGGATCAAAA GGCCCAGCAA GCCTCCAGTG 
CACGAGGAAC CCAAGGGAGG CCTGAAGGTG GAGAGCGAGC CCGCTCCTTA TGAGATTGGA 
GAGCAGTCTT CCAGAGACAA GCCAAAACCC AAAACAAAAA GCAGATCCAA ATCCTGTGAC 
CAAAAAGAAC TGAAACCCCG GCTGCAGGAG GCTCCTAAAG AGAAGAAGCA CAAGAGCTCC 
CACCACGCCG ATGCCAATGC CCTGCCGGAC CACAGACCCG TGAGTGATGT TCTGGTAGGC 
AGTGCCCAGG AGCCTTCCAC TCTCAAACAC CTCCCTGAGG GCCAGGGCAC AGCTCCCACC 
AGGACTAACG GCCACAGGTC TGCTGCTGAG GTCAAGGAGG GTTTTCACAA GGGGAAGCTT 
CCTGTGCCCA CCAAGGAGAA GAAGTTGCTG CCACCGGTGA GGGACGTGCC TGGCAATCCC 
AGCCTCCTGG TTAGAATTGA TCTCCCACTC CTGTCAAGAG TTCCCCAGCC TCCTGAGAAG 
GGGAACCAAA AGAAGAGAGC AGAGGTGAAG GAGCACCCTG GCACCAAGGA GCAGGACTTG 
GAGAAGAAAA GCACAGACAC TCCTGACAGG TCCTTCAAAA AGCGAAAGAG AGAAAAGGAG 
AAGGACGTCG ATAGGAAGAA AAGGAAGTTG GGCAAAGAGG CAAAATCATT GCAGTCTTCA 
CCTAACAAAG ACTCCTGTAA ATTGAAAGCA CGGAACGCTT CGCCTGAGAC CCAGAACAAA 
GATCTCAGGC AACCCCTGCC ACTGCCGCCC ATGTCTCCCA CGCATCCTGC ACCAAAGCCA 
ACCAAGAAGG CCCACAAGAG GCCCAGGAGT GAGAACGGCC AGCCGCCTGC CACTGCCAGC 
AGCACTGCCC TGGATGCAAG CAACCACAAG GATCCTTCTT CCACCAAGCA GAAGAAGATG 
GTGGAAGACC ACTCTGAGCA GGTGAAGGGC AACAAAGGAT CTGCAGGGGA TGTCACAAAT 
CCTTTACCAG TGCCTTCTGT GCCAAATGGT ACCTCCAAAC CAAGGAGACC TCAGATCAAG 
TTTGAAAGAC AGCATTTGTC AGAATATTAC CTGGAAGAGG CCAAGAAGAT GAAGCACGAG 
GCTGATGCCA TGATGGACAA GACTGGAAAG GCCTTTCAGT ACCTCGATGC TGCCCTTTCC 
TTTGTTGAAT ACGGGATGGC CCTGGAATCG AATGCGGTAG CACCAAAACC TGCTTACAGC 
ATCTTTGCTG ACACAATAGA TCTCCTCAAA TACGTAATGA CACTAAAACC CTTTACGGAT 
GTCTCGGCAT CTTCACATGA ACACGTCTTT GCTGTGTTAT GCATGCGCTG CCAGTCCCTC 
CTGCACATGG CAATGTTTCG TTACAAAAGA GACACTGCAA TAAAGTATTC CAGGATCCTG 
AATGACCACT TCAAGAGTTC TTCCAGAGTA ACACAGGCAC CTTCTCCATG TGTTGCAAGA 
AGCACAGGCA TGCCTTCTCC TCATTCTCCA GTGCCCTCTC CTGCCAGATC TATGAGTTCT 
CAGCAGGGAT CAAATGGTAG CAATTGTGGT TGCAACAGCA TTGGCTCTTC AGTCACCATC 
CCAAGCATCA CCTCTTCATA CGTCAACATC ACTTCCTATG TCCTCTATGC GTATAATATT 
TGGGAACATG CTGATGCACT GTCCAAGAAT AATAAGGAGT TTTTTGCTGA ACTCAGCACA 
GCGGTGTGCC CTCTGGCACT GACCAGTAGC ATGGCCGAAA TGGTACACTA CACTAGACAG 
GGTTTACATT GGCTGAGACT GAACACAAGT ACGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004941155.1
CDS	4..3762
Translation	MMKTMAESCQRKVWLGERLVLKEVVKKPGLESPLQPEMGLRPKGASAAFPGSLYNEDRNLLRLQERQRRNQEALQEKDKFLENTPLFPEPYKTNKEDELSSRIQNMLGNYDDLQDLINDRCHQNLIGIPESDVPQFPQRKPDRPSFPEKTSRTLSSSFHHATRYPHMGPPVIAPPRPGHYAPHQKAQPGTGPAPGWHAKSHSSSSSWSQGQEHKRGRPDSHAGSHHNRTDRHPAGGGRAPEPQATLLELSPLLPSLSSPVAPLSPLHSIQHTNSKSQKSSKGHGPAYTQSPQDRAVGSRENKSQDNSAVILAGTTQPSSQPFPPPLPSKTSAMQQKPTAYVRPMDGQEQAPVESPDLKPLLEEYHGEPYENISDLKANTKAKLSKLNISSEPTEQTLPSDSQCVEEILKEMTHSWPPLLTAIHTPSAAEPSKFPFPTKESQHIPSVAQNHKQYDAPSKTLPSSHPTISVLQEDLQLSDSEESGDDQVVEQPPSSLDPPSALQSQPESVASAHSNSTESGSSSDSDSSSDSSETESSSSDSEANDPPRASAPEPDPPTSNKWQLDNWLTKVNPPAVPTESPSDTAHTDGHEEDKEQPVSNASSCEHAVPREPHERSSIRAAQAPQDVHLPTKLSCQKSPVRAEEPSQRQTVGIKRPSKPPVHEEPKGGLKVESEPAPYEIGEQSSRDKPKPKTKSRSKSCDQKELKPRLQEAPKEKKHKSSHHADANALPDHRPVSDVLVGSAQEPSTLKHLPEGQGTAPTRTNGHRSAAEVKEGFHKGKLPVPTKEKKLLPPVRDVPGNPSLLVRIDLPLLSRVPQPPEKGNQKKRAEVKEHPGTKEQDLEKKSTDTPDRSFKKRKREKEKDVDRKKRKLGKEAKSLQSSPNKDSCKLKARNASPETQNKDLRQPLPLPPMSPTHPAPKPTKKAHKRPRSENGQPPATASSTALDASNHKDPSSTKQKKMVEDHSEQVKGNKGSAGDVTNPLPVPSVPNGTSKPRRPQIKFERQHLSEYYLEEAKKMKHEADAMMDKTGKAFQYLDAALSFVEYGMALESNAVAPKPAYSIFADTIDLLKYVMTLKPFTDVSASSHEHVFAVLCMRCQSLLHMAMFRYKRDTAIKYSRILNDHFKSSSRVTQAPSPCVARSTGMPSPHSPVPSPARSMSSQQGSNGSNCGCNSIGSSVTIPSITSSYVNITSYVLYAYNIWEHADALSKNNKEFFAELSTAVCPLALTSSMAEMVHYTRQGLHWLRLNTSTP

Target ORF information:

RefSeq Version	XM_004941098.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004941098.3

ORF Insert Sequence:

ATGATGAAAA CGATGGCTGA GAGCTGCCAG AGGAAAGTTT GGCTGGGAGA ACGGCTGGTG 
CTAAAGGAGG TGGTGAAGAA ACCTGGTTTA GAAAGTCCAT TGCAGCCTGA GATGGGATTA 
CGTCCAAAAG GTGCCTCTGC TGCTTTCCCT GGGAGCTTGT ACAATGAGGA CAGAAACTTG 
CTGCGTCTTC AGGAGAGACA GAGGCGGAAT CAGGAGGCTC TGCAGGAGAA AGACAAGTTC 
CTTGAGAACA CCCCACTCTT TCCTGAGCCG TACAAGACTA ATAAAGAGGA TGAGTTGTCA 
AGCCGTATTC AGAATATGCT GGGCAATTAC GACGACTTGC AGGATCTTAT TAATGACAGA 
TGCCATCAGA ATCTCATAGG GATACCAGAG AGCGACGTTC CTCAGTTCCC ACAAAGAAAG 
CCCGACCGCC CATCCTTCCC TGAAAAGACC AGCCGTACGT TATCGTCTTC ATTCCACCAT 
GCAACCCGCT ATCCACACAT GGGACCCCCG GTCATTGCTC CTCCCCGTCC AGGCCACTAC 
GCTCCCCACC AAAAGGCACA ACCAGGAACA GGACCAGCTC CAGGCTGGCA TGCCAAGAGT 
CACAGCTCGT CCAGCAGCTG GAGCCAAGGT CAGGAACACA AGCGTGGCAG GCCGGACAGT 
CATGCCGGTT CTCACCACAA CAGAACTGAC AGGCATCCTG CTGGTGGAGG TCGTGCTCCT 
GAACCTCAGG CCACTCTTTT GGAGCTTTCC CCGTTGCTGC CGTCTTTGTC TTCTCCAGTG 
GCACCCCTGT CACCTCTACA TTCCATCCAG CACACTAATT CCAAGTCACA GAAGAGCAGC 
AAAGGTCATG GGCCGGCCTA CACTCAATCA CCTCAGGACC GAGCGGTGGG TTCACGGGAG 
AATAAAAGTC AGGACAACTC AGCCGTTATC TTGGCTGGCA CCACTCAGCC TTCCTCTCAA 
CCCTTTCCTC CACCTTTGCC ATCAAAAACC AGTGCAATGC AGCAGAAACC AACTGCCTAC 
GTCAGACCAA TGGATGGCCA AGAGCAGGCT CCGGTGGAGT CACCGGATCT CAAGCCTCTA 
CTGGAGGAAT ACCACGGTGA ACCGTATGAA AACATCTCAG ATTTGAAGGC AAACACCAAG 
GCCAAATTAT CCAAATTGAA CATCTCTTCC GAGCCCACTG AGCAAACCTT GCCCAGTGAT 
AGCCAGTGCG TTGAAGAAAT TTTGAAGGAA ATGACACACT CATGGCCACC TCTCTTGACA 
GCTATTCACA CGCCAAGTGC TGCAGAGCCC TCCAAGTTTC CCTTTCCAAC AAAGGAATCG 
CAGCATATCC CATCTGTAGC ACAGAATCAC AAGCAGTATG ACGCACCTTC CAAAACGTTG 
CCTAGTTCTC ATCCAACAAT ATCAGTGCTC CAGGAGGACC TGCAGCTCAG TGATAGTGAA 
GAGAGTGGTG ACGACCAAGT TGTTGAACAG CCACCTTCTT CACTTGATCC TCCAAGTGCC 
CTGCAGTCCC AGCCTGAGAG CGTGGCATCA GCACATTCCA ACAGCACAGA GTCAGGGAGC 
AGCAGTGACT CAGACAGCTC TTCAGACTCT TCAGAGACAG AGAGCAGCTC GAGTGACAGT 
GAAGCCAACG ACCCTCCAAG AGCGTCAGCT CCTGAGCCCG ACCCACCAAC TTCCAATAAG 
TGGCAGCTTG ACAACTGGCT CACCAAGGTG AACCCACCAG CAGTGCCAAC AGAAAGCCCC 
AGCGACACTG CCCACACAGA TGGCCACGAG GAGGACAAGG AACAACCCGT CAGCAACGCT 
TCCTCCTGCG AGCATGCTGT GCCAAGGGAA CCTCACGAGA GAAGCTCCAT CCGAGCAGCC 
CAGGCTCCTC AGGATGTTCA TCTTCCGACC AAACTTAGCT GCCAGAAGTC TCCTGTGCGC 
GCTGAGGAGC CCTCACAGAG GCAGACTGTG GGGATCAAAA GGCCCAGCAA GCCTCCAGTG 
CACGAGGAAC CCAAGGGAGG CCTGAAGGTG GAGAGCGAGC CCGCTCCTTA TGAGATTGGA 
GAGCAGTCTT CCAGAGACAA GCCAAAACCC AAAACAAAAA GCAGATCCAA ATCCTGTGAC 
CAAAAAGAAC TGAAACCCCG GCTGCAGGAG GCTCCTAAAG AGAAGAAGCA CAAGAGCTCC 
CACCACGCCG ATGCCAATGC CCTGCCGGAC CACAGACCCG TGAGTGATGT TCTGGTAGGC 
AGTGCCCAGG AGCCTTCCAC TCTCAAACAC CTCCCTGAGG GCCAGGGCAC AGCTCCCACC 
AGGACTAACG GCCACAGGTC TGCTGCTGAG GTCAAGGAGG GTTTTCACAA GGGGAAGCTT 
CCTGTGCCCA CCAAGGAGAA GAAGTTGCTG CCACCGGTGA GGGACGTGCC TGGCAATCCC 
AGCCTCCTGG TTAGAATTGA TCTCCCACTC CTGTCAAGAG TTCCCCAGCC TCCTGAGAAG 
GGGAACCAAA AGAAGAGAGC AGAGGTGAAG GAGCACCCTG GCACCAAGGA GCAGGACTTG 
GAGAAGAAAA GCACAGACAC TCCTGACAGG TCCTTCAAAA AGCGAAAGAG AGAAAAGGAG 
AAGGACGTCG ATAGGAAGAA AAGGAAGTTG GGCAAAGAGG CAAAATCATT GCAGTCTTCA 
CCTAACAAAG ACTCCTGTAA ATTGAAAGCA CGGAACGCTT CGCCTGAGAC CCAGAACAAA 
GATCTCAGGC AACCCCTGCC ACTGCCGCCC ATGTCTCCCA CGCATCCTGC ACCAAAGCCA 
ACCAAGAAGG CCCACAAGAG GCCCAGGAGT GAGAACGGCC AGCCGCCTGC CACTGCCAGC 
AGCACTGCCC TGGATGCAAG CAACCACAAG GATCCTTCTT CCACCAAGCA GAAGAAGATG 
GTGGAAGACC ACTCTGAGCA GGTGAAGGGC AACAAAGGAT CTGCAGGGGA TGTCACAAAT 
CCTTTACCAG TGCCTTCTGT GCCAAATGGT ACCTCCAAAC CAAGGAGACC TCAGATCAAG 
TTTGAAAGAC AGCATTTGTC AGAATATTAC CTGGAAGAGG CCAAGAAGAT GAAGCACGAG 
GCTGATGCCA TGATGGACAA GACTGGAAAG GCCTTTCAGT ACCTCGATGC TGCCCTTTCC 
TTTGTTGAAT ACGGGATGGC CCTGGAATCG AATGCGGTAG CACCAAAACC TGCTTACAGC 
ATCTTTGCTG ACACAATAGA TCTCCTCAAA TACGTAATGA CACTAAAACC CTTTACGGAT 
GTCTCGGCAT CTTCACATGA ACACGTCTTT GCTGTGTTAT GCATGCGCTG CCAGTCCCTC 
CTGCACATGG CAATGTTTCG TTACAAAAGA GACACTGCAA TAAAGTATTC CAGGATCCTG 
AATGACCACT TCAAGAGTTC TTCCAGAGTA ACACAGGCAC CTTCTCCATG TGTTGCAAGA 
AGCACAGGCA TGCCTTCTCC TCATTCTCCA GTGCCCTCTC CTGCCAGATC TATGAGTTCT 
CAGCAGGGAT CAAATGGTAG CAATTGTGGT TGCAACAGCA TTGGCTCTTC AGTCACCATC 
CCAAGCATCA CCTCTTCATA CGTCAACATC ACTTCCTATG TCCTCTATGC GTATAATATT 
TGGGAACATG CTGATGCACT GTCCAAGAAT AATAAGGAGT TTTTTGCTGA ACTCAGCACA 
GCGGTGTGCC CTCTGGCACT GACCAGTAGC ATGGCCGAAA TGGTACACTA CACTAGACAG 
GGTTTACATT GGCTGAGACT GAACACAAGT ACGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_420549.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_420549.4 Gallus gallus AF4/FMR2 family, member 1 (AFF1), transcript variant X2, mRNA.

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CloneID	OGa08002
Clone ID Related Accession (Same CDS sequence)	XM_420549.5 , XM_420549.6
Accession Version	XM_420549.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	AF4/FMR2 family member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_420549.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAA CGATGGCTGA GAGCTGCCAG AGGAAAGTTT GGCTGGGAGA ACGGCTGGTG 
CTAAAGGAGG TGGTGAAGAA ACCTGGTTTA GAAAGTCCAT TGCAGCCTGA GATGGGATTA 
CGTCCAAAAG GTGCCTCTGC TGCTTTCCCT GGGAGCTTGT ACAATGAGGA CAGAAACTTG 
CTGCGTCTTC AGGAGAGACA GAGGCGGAAT CAGGAGGCTC TGCAGGAGAA AGACAAGTTC 
CTTGAGAACA CCCCACTCTT TCCTGAGCCG TACAAGACTA ATAAAGAGGA TGAGTTGTCA 
AGCCGTATTC AGAATATGCT GGGCAATTAC GACGACTTGC AGGATCTTAT TAATGACAGA 
TGCCATCAGA ATCTCATAGG GATACCAGAG AGCGACGTTC CTCAGTTCCC ACAAAGAAAG 
CCCGACCGCC CATCCTTCCC TGAAAAGACC AGCCGTACGT TATCGTCTTC ATTCCACCAT 
GCAACCCGCT ATCCACACAT GGGACCCCCG GTCATTGCTC CTCCCCGTCC AGGCCACTAC 
GCTCCCCACC AAAAGGCACA ACCAGGAACA GGACCAGCTC CAGGCTGGCA TGCCAAGAGT 
CACAGCTCGT CCAGCAGCTG GAGCCAAGGT CAGGAACACA AGCGTGGCAG GCCGGACAGT 
CATGCCGGTT CTCACCACAA CAGAACTGAC AGGCATCCTG CTGGTGGAGG TCGTGCTCCT 
GAACCTCAGG CCACTCTTTT GGAGCTTTCC CCGTTGCTGC CGTCTTTGTC TTCTCCAGTG 
GCACCCCTGT CACCTCTACA TTCCATCCAG CACACTAATT CCAAGTCACA GAAGAGCAGC 
AAAGGTCATG GGCCGGCCTA CACTCAATCA CCTCAGGACC GAGCGGTGGG TTCACGGGAG 
AATAAAAGTC AGGACAACTC AGCCGTTATC TTGGCTGGCA CCACTCAGCC TTCCTCTCAA 
CCCTTTCCTC CACCTTTGCC ATCAAAAACC AGTGCAATGC AGCAGAAACC AACTGCCTAC 
GTCAGACCAA TGGATGGCCA AGAGCAGGCT CCGGTGGAGT CACCGGATCT CAAGCCTCTA 
CTGGAGGAAT ACCACGGTGA ACCGTATGAA AACATCTCAG ATTTGAAGGC AAACACCAAG 
GCCAAATTAT CCAAATTGAA CATCTCTTCC GAGCCCACTG AGCAAACCTT GCCCAGTGAT 
AGCCAGTGCG TTGAAGAAAT TTTGAAGGAA ATGACACACT CATGGCCACC TCTCTTGACA 
GCTATTCACA CGCCAAGTGC TGCAGAGCCC TCCAAGTTTC CCTTTCCAAC AAAGGAATCG 
CAGCATATCC CATCTGTAGC ACAGAATCAC AAGCAGTATG ACGCACCTTC CAAAACGTTG 
CCTAGTTCTC ATCCAACAAT ATCAGTGCTC CAGGAGGACC TGCAGCTCAG TGATAGTGAA 
GAGAGTGGTG ACGACCAAGT TGTTGAACAG CCACCTTCTT CACTTGATCC TCCAAGTGCC 
CTGCAGTCCC AGCCTGAGAG CGTGGCATCA GCACATTCCA ACAGCACAGA GTCAGGGAGC 
AGCAGTGACT CAGACAGCTC TTCAGACTCT TCAGAGACAG AGAGCAGCTC GAGTGACAGT 
GAAGCCAACG ACCCTCCAAG AGCGTCAGCT CCTGAGCCCG ACCCACCAAC TTCCAATAAG 
TGGCAGCTTG ACAACTGGCT CACCAAGGTG AACCCACCAG CAGTGCCAAC AGAAAGCCCC 
AGCGACACTG CCCACACAGA TGGCCACGAG GAGGACAAGG AACAACCCGT CAGCAACGCT 
TCCTCCTGCG AGCATGCTGT GCCAAGGGAA CCTCACGAGA GAAGCTCCAT CCGAGCAGCC 
CAGGCTCCTC AGGATGTTCA TCTTCCGACC AAACTTAGCT GCCAGAAGTC TCCTGTGCGC 
GCTGAGGAGC CCTCACAGAG GCAGACTGTG GGGATCAAAA GGCCCAGCAA GCCTCCAGTG 
CACGAGGAAC CCAAGGGAGG CCTGAAGGTG GAGAGCGAGC CCGCTCCTTA TGAGATTGGA 
GAGCAGTCTT CCAGAGACAA GCCAAAACCC AAAACAAAAA GCAGATCCAA ATCCTGTGAC 
CAAAAAGAAC TGAAACCCCG GCTGCAGGAG GCTCCTAAAG AGAAGAAGCA CAAGAGCTCC 
CACCACGCCG ATGCCAATGC CCTGCCGGAC CACAGACCCG TGAGTGATGT TCTGGTAGGC 
AGTGCCCAGG AGCCTTCCAC TCTCAAACAC CTCCCTGAGG GCCAGGGCAC AGCTCCCACC 
AGGACTAACG GCCACAGGTC TGCTGCTGAG GTCAAGGAGG GTTTTCACAA GGGGAAGCTT 
CCTGTGCCCA CCAAGGAGAA GAAGTTGCTG CCACCGGTGA GGGACGTGCC TGGCAATCCC 
AGCCTCCTGG TTAGAATTGA TCTCCCACTC CTGTCAAGAG TTCCCCAGCC TCCTGAGAAG 
GGGAACCAAA AGAAGAGAGC AGAGGTGAAG GAGCACCCTG GCACCAAGGA GCAGGACTTG 
GAGAAGAAAA GCACAGACAC TCCTGACAGG TCCTTCAAAA AGCGAAAGAG AGAAAAGGAG 
AAGGACGTCG ATAGGAAGAA AAGGAAGTTG GGCAAAGAGG CAAAATCATT GCAGTCTTCA 
CCTAACAAAG ACTCCTGTAA ATTGAAAGCA CGGAACGCTT CGCCTGAGAC CCAGAACAAA 
GATCTCAGGC AACCCCTGCC ACTGCCGCCC ATGTCTCCCA CGCATCCTGC ACCAAAGCCA 
ACCAAGAAGG CCCACAAGAG GCCCAGGAGT GAGAACGGCC AGCCGCCTGC CACTGCCAGC 
AGCACTGCCC TGGATGCAAG CAACCACAAG GATCCTTCTT CCACCAAGCA GAAGAAGATG 
GTGGAAGACC ACTCTGAGCA GGGATCTGCA GGGGATGTCA CAAATCCTTT ACCAGTGCCT 
TCTGTGCCAA ATGGTACCTC CAAACCAAGG AGACCTCAGA TCAAGTTTGA AAGACAGCAT 
TTGTCAGAAT ATTACCTGGA AGAGGCCAAG AAGATGAAGC ACGAGGCTGA TGCCATGATG 
GACAAGACTG GAAAGGCCTT TCAGTACCTC GATGCTGCCC TTTCCTTTGT TGAATACGGG 
ATGGCCCTGG AATCGAATGC GGTAGCACCA AAACCTGCTT ACAGCATCTT TGCTGACACA 
ATAGATCTCC TCAAATACGT AATGACACTA AAACCCTTTA CGGATGTCTC GGCATCTTCA 
CATGAACACG TCTTTGCTGT GTTATGCATG CGCTGCCAGT CCCTCCTGCA CATGGCAATG 
TTTCGTTACA AAAGAGACAC TGCAATAAAG TATTCCAGGA TCCTGAATGA CCACTTCAAG 
AGTTCTTCCA GAGTAACACA GGCACCTTCT CCATGTGTTG CAAGAAGCAC AGGCATGCCT 
TCTCCTCATT CTCCAGTGCC CTCTCCTGCC AGATCTATGA GTTCTCAGCA GGGATCAAAT 
GGTAGCAATT GTGGTTGCAA CAGCATTGGC TCTTCAGTCA CCATCCCAAG CATCACCTCT 
TCATACGTCA ACATCACTTC CTATGTCCTC TATGCGTATA ATATTTGGGA ACATGCTGAT 
GCACTGTCCA AGAATAATAA GGAGTTTTTT GCTGAACTCA GCACAGCGGT GTGCCCTCTG 
GCACTGACCA GTAGCATGGC CGAAATGGTA CACTACACTA GACAGGGTTT ACATTGGCTG 
AGACTGAACA CAAGTACGCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_420549.4
CDS	4..3747
Translation	MMKTMAESCQRKVWLGERLVLKEVVKKPGLESPLQPEMGLRPKGASAAFPGSLYNEDRNLLRLQERQRRNQEALQEKDKFLENTPLFPEPYKTNKEDELSSRIQNMLGNYDDLQDLINDRCHQNLIGIPESDVPQFPQRKPDRPSFPEKTSRTLSSSFHHATRYPHMGPPVIAPPRPGHYAPHQKAQPGTGPAPGWHAKSHSSSSSWSQGQEHKRGRPDSHAGSHHNRTDRHPAGGGRAPEPQATLLELSPLLPSLSSPVAPLSPLHSIQHTNSKSQKSSKGHGPAYTQSPQDRAVGSRENKSQDNSAVILAGTTQPSSQPFPPPLPSKTSAMQQKPTAYVRPMDGQEQAPVESPDLKPLLEEYHGEPYENISDLKANTKAKLSKLNISSEPTEQTLPSDSQCVEEILKEMTHSWPPLLTAIHTPSAAEPSKFPFPTKESQHIPSVAQNHKQYDAPSKTLPSSHPTISVLQEDLQLSDSEESGDDQVVEQPPSSLDPPSALQSQPESVASAHSNSTESGSSSDSDSSSDSSETESSSSDSEANDPPRASAPEPDPPTSNKWQLDNWLTKVNPPAVPTESPSDTAHTDGHEEDKEQPVSNASSCEHAVPREPHERSSIRAAQAPQDVHLPTKLSCQKSPVRAEEPSQRQTVGIKRPSKPPVHEEPKGGLKVESEPAPYEIGEQSSRDKPKPKTKSRSKSCDQKELKPRLQEAPKEKKHKSSHHADANALPDHRPVSDVLVGSAQEPSTLKHLPEGQGTAPTRTNGHRSAAEVKEGFHKGKLPVPTKEKKLLPPVRDVPGNPSLLVRIDLPLLSRVPQPPEKGNQKKRAEVKEHPGTKEQDLEKKSTDTPDRSFKKRKREKEKDVDRKKRKLGKEAKSLQSSPNKDSCKLKARNASPETQNKDLRQPLPLPPMSPTHPAPKPTKKAHKRPRSENGQPPATASSTALDASNHKDPSSTKQKKMVEDHSEQGSAGDVTNPLPVPSVPNGTSKPRRPQIKFERQHLSEYYLEEAKKMKHEADAMMDKTGKAFQYLDAALSFVEYGMALESNAVAPKPAYSIFADTIDLLKYVMTLKPFTDVSASSHEHVFAVLCMRCQSLLHMAMFRYKRDTAIKYSRILNDHFKSSSRVTQAPSPCVARSTGMPSPHSPVPSPARSMSSQQGSNGSNCGCNSIGSSVTIPSITSSYVNITSYVLYAYNIWEHADALSKNNKEFFAELSTAVCPLALTSSMAEMVHYTRQGLHWLRLNTSTP

Target ORF information:

RefSeq Version	XM_420549.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus AF4/FMR2 family, member 1 (AFF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_420549.5

ORF Insert Sequence:

ATGATGAAAA CGATGGCTGA GAGCTGCCAG AGGAAAGTTT GGCTGGGAGA ACGGCTGGTG 
CTAAAGGAGG TGGTGAAGAA ACCTGGTTTA GAAAGTCCAT TGCAGCCTGA GATGGGATTA 
CGTCCAAAAG GTGCCTCTGC TGCTTTCCCT GGGAGCTTGT ACAATGAGGA CAGAAACTTG 
CTGCGTCTTC AGGAGAGACA GAGGCGGAAT CAGGAGGCTC TGCAGGAGAA AGACAAGTTC 
CTTGAGAACA CCCCACTCTT TCCTGAGCCG TACAAGACTA ATAAAGAGGA TGAGTTGTCA 
AGCCGTATTC AGAATATGCT GGGCAATTAC GACGACTTGC AGGATCTTAT TAATGACAGA 
TGCCATCAGA ATCTCATAGG GATACCAGAG AGCGACGTTC CTCAGTTCCC ACAAAGAAAG 
CCCGACCGCC CATCCTTCCC TGAAAAGACC AGCCGTACGT TATCGTCTTC ATTCCACCAT 
GCAACCCGCT ATCCACACAT GGGACCCCCG GTCATTGCTC CTCCCCGTCC AGGCCACTAC 
GCTCCCCACC AAAAGGCACA ACCAGGAACA GGACCAGCTC CAGGCTGGCA TGCCAAGAGT 
CACAGCTCGT CCAGCAGCTG GAGCCAAGGT CAGGAACACA AGCGTGGCAG GCCGGACAGT 
CATGCCGGTT CTCACCACAA CAGAACTGAC AGGCATCCTG CTGGTGGAGG TCGTGCTCCT 
GAACCTCAGG CCACTCTTTT GGAGCTTTCC CCGTTGCTGC CGTCTTTGTC TTCTCCAGTG 
GCACCCCTGT CACCTCTACA TTCCATCCAG CACACTAATT CCAAGTCACA GAAGAGCAGC 
AAAGGTCATG GGCCGGCCTA CACTCAATCA CCTCAGGACC GAGCGGTGGG TTCACGGGAG 
AATAAAAGTC AGGACAACTC AGCCGTTATC TTGGCTGGCA CCACTCAGCC TTCCTCTCAA 
CCCTTTCCTC CACCTTTGCC ATCAAAAACC AGTGCAATGC AGCAGAAACC AACTGCCTAC 
GTCAGACCAA TGGATGGCCA AGAGCAGGCT CCGGTGGAGT CACCGGATCT CAAGCCTCTA 
CTGGAGGAAT ACCACGGTGA ACCGTATGAA AACATCTCAG ATTTGAAGGC AAACACCAAG 
GCCAAATTAT CCAAATTGAA CATCTCTTCC GAGCCCACTG AGCAAACCTT GCCCAGTGAT 
AGCCAGTGCG TTGAAGAAAT TTTGAAGGAA ATGACACACT CATGGCCACC TCTCTTGACA 
GCTATTCACA CGCCAAGTGC TGCAGAGCCC TCCAAGTTTC CCTTTCCAAC AAAGGAATCG 
CAGCATATCC CATCTGTAGC ACAGAATCAC AAGCAGTATG ACGCACCTTC CAAAACGTTG 
CCTAGTTCTC ATCCAACAAT ATCAGTGCTC CAGGAGGACC TGCAGCTCAG TGATAGTGAA 
GAGAGTGGTG ACGACCAAGT TGTTGAACAG CCACCTTCTT CACTTGATCC TCCAAGTGCC 
CTGCAGTCCC AGCCTGAGAG CGTGGCATCA GCACATTCCA ACAGCACAGA GTCAGGGAGC 
AGCAGTGACT CAGACAGCTC TTCAGACTCT TCAGAGACAG AGAGCAGCTC GAGTGACAGT 
GAAGCCAACG ACCCTCCAAG AGCGTCAGCT CCTGAGCCCG ACCCACCAAC TTCCAATAAG 
TGGCAGCTTG ACAACTGGCT CACCAAGGTG AACCCACCAG CAGTGCCAAC AGAAAGCCCC 
AGCGACACTG CCCACACAGA TGGCCACGAG GAGGACAAGG AACAACCCGT CAGCAACGCT 
TCCTCCTGCG AGCATGCTGT GCCAAGGGAA CCTCACGAGA GAAGCTCCAT CCGAGCAGCC 
CAGGCTCCTC AGGATGTTCA TCTTCCGACC AAACTTAGCT GCCAGAAGTC TCCTGTGCGC 
GCTGAGGAGC CCTCACAGAG GCAGACTGTG GGGATCAAAA GGCCCAGCAA GCCTCCAGTG 
CACGAGGAAC CCAAGGGAGG CCTGAAGGTG GAGAGCGAGC CCGCTCCTTA TGAGATTGGA 
GAGCAGTCTT CCAGAGACAA GCCAAAACCC AAAACAAAAA GCAGATCCAA ATCCTGTGAC 
CAAAAAGAAC TGAAACCCCG GCTGCAGGAG GCTCCTAAAG AGAAGAAGCA CAAGAGCTCC 
CACCACGCCG ATGCCAATGC CCTGCCGGAC CACAGACCCG TGAGTGATGT TCTGGTAGGC 
AGTGCCCAGG AGCCTTCCAC TCTCAAACAC CTCCCTGAGG GCCAGGGCAC AGCTCCCACC 
AGGACTAACG GCCACAGGTC TGCTGCTGAG GTCAAGGAGG GTTTTCACAA GGGGAAGCTT 
CCTGTGCCCA CCAAGGAGAA GAAGTTGCTG CCACCGGTGA GGGACGTGCC TGGCAATCCC 
AGCCTCCTGG TTAGAATTGA TCTCCCACTC CTGTCAAGAG TTCCCCAGCC TCCTGAGAAG 
GGGAACCAAA AGAAGAGAGC AGAGGTGAAG GAGCACCCTG GCACCAAGGA GCAGGACTTG 
GAGAAGAAAA GCACAGACAC TCCTGACAGG TCCTTCAAAA AGCGAAAGAG AGAAAAGGAG 
AAGGACGTCG ATAGGAAGAA AAGGAAGTTG GGCAAAGAGG CAAAATCATT GCAGTCTTCA 
CCTAACAAAG ACTCCTGTAA ATTGAAAGCA CGGAACGCTT CGCCTGAGAC CCAGAACAAA 
GATCTCAGGC AACCCCTGCC ACTGCCGCCC ATGTCTCCCA CGCATCCTGC ACCAAAGCCA 
ACCAAGAAGG CCCACAAGAG GCCCAGGAGT GAGAACGGCC AGCCGCCTGC CACTGCCAGC 
AGCACTGCCC TGGATGCAAG CAACCACAAG GATCCTTCTT CCACCAAGCA GAAGAAGATG 
GTGGAAGACC ACTCTGAGCA GGGATCTGCA GGGGATGTCA CAAATCCTTT ACCAGTGCCT 
TCTGTGCCAA ATGGTACCTC CAAACCAAGG AGACCTCAGA TCAAGTTTGA AAGACAGCAT 
TTGTCAGAAT ATTACCTGGA AGAGGCCAAG AAGATGAAGC ACGAGGCTGA TGCCATGATG 
GACAAGACTG GAAAGGCCTT TCAGTACCTC GATGCTGCCC TTTCCTTTGT TGAATACGGG 
ATGGCCCTGG AATCGAATGC GGTAGCACCA AAACCTGCTT ACAGCATCTT TGCTGACACA 
ATAGATCTCC TCAAATACGT AATGACACTA AAACCCTTTA CGGATGTCTC GGCATCTTCA 
CATGAACACG TCTTTGCTGT GTTATGCATG CGCTGCCAGT CCCTCCTGCA CATGGCAATG 
TTTCGTTACA AAAGAGACAC TGCAATAAAG TATTCCAGGA TCCTGAATGA CCACTTCAAG 
AGTTCTTCCA GAGTAACACA GGCACCTTCT CCATGTGTTG CAAGAAGCAC AGGCATGCCT 
TCTCCTCATT CTCCAGTGCC CTCTCCTGCC AGATCTATGA GTTCTCAGCA GGGATCAAAT 
GGTAGCAATT GTGGTTGCAA CAGCATTGGC TCTTCAGTCA CCATCCCAAG CATCACCTCT 
TCATACGTCA ACATCACTTC CTATGTCCTC TATGCGTATA ATATTTGGGA ACATGCTGAT 
GCACTGTCCA AGAATAATAA GGAGTTTTTT GCTGAACTCA GCACAGCGGT GTGCCCTCTG 
GCACTGACCA GTAGCATGGC CGAAATGGTA CACTACACTA GACAGGGTTT ACATTGGCTG 
AGACTGAACA CAAGTACGCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_420549.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_420549.4 Gallus gallus AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA.

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CloneID	OGa08002
Clone ID Related Accession (Same CDS sequence)	XM_420549.5 , XM_420549.6
Accession Version	XM_420549.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	AF4/FMR2 family member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_420549.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAA CGATGGCTGA GAGCTGCCAG AGGAAAGTTT GGCTGGGAGA ACGGCTGGTG 
CTAAAGGAGG TGGTGAAGAA ACCTGGTTTA GAAAGTCCAT TGCAGCCTGA GATGGGATTA 
CGTCCAAAAG GTGCCTCTGC TGCTTTCCCT GGGAGCTTGT ACAATGAGGA CAGAAACTTG 
CTGCGTCTTC AGGAGAGACA GAGGCGGAAT CAGGAGGCTC TGCAGGAGAA AGACAAGTTC 
CTTGAGAACA CCCCACTCTT TCCTGAGCCG TACAAGACTA ATAAAGAGGA TGAGTTGTCA 
AGCCGTATTC AGAATATGCT GGGCAATTAC GACGACTTGC AGGATCTTAT TAATGACAGA 
TGCCATCAGA ATCTCATAGG GATACCAGAG AGCGACGTTC CTCAGTTCCC ACAAAGAAAG 
CCCGACCGCC CATCCTTCCC TGAAAAGACC AGCCGTACGT TATCGTCTTC ATTCCACCAT 
GCAACCCGCT ATCCACACAT GGGACCCCCG GTCATTGCTC CTCCCCGTCC AGGCCACTAC 
GCTCCCCACC AAAAGGCACA ACCAGGAACA GGACCAGCTC CAGGCTGGCA TGCCAAGAGT 
CACAGCTCGT CCAGCAGCTG GAGCCAAGGT CAGGAACACA AGCGTGGCAG GCCGGACAGT 
CATGCCGGTT CTCACCACAA CAGAACTGAC AGGCATCCTG CTGGTGGAGG TCGTGCTCCT 
GAACCTCAGG CCACTCTTTT GGAGCTTTCC CCGTTGCTGC CGTCTTTGTC TTCTCCAGTG 
GCACCCCTGT CACCTCTACA TTCCATCCAG CACACTAATT CCAAGTCACA GAAGAGCAGC 
AAAGGTCATG GGCCGGCCTA CACTCAATCA CCTCAGGACC GAGCGGTGGG TTCACGGGAG 
AATAAAAGTC AGGACAACTC AGCCGTTATC TTGGCTGGCA CCACTCAGCC TTCCTCTCAA 
CCCTTTCCTC CACCTTTGCC ATCAAAAACC AGTGCAATGC AGCAGAAACC AACTGCCTAC 
GTCAGACCAA TGGATGGCCA AGAGCAGGCT CCGGTGGAGT CACCGGATCT CAAGCCTCTA 
CTGGAGGAAT ACCACGGTGA ACCGTATGAA AACATCTCAG ATTTGAAGGC AAACACCAAG 
GCCAAATTAT CCAAATTGAA CATCTCTTCC GAGCCCACTG AGCAAACCTT GCCCAGTGAT 
AGCCAGTGCG TTGAAGAAAT TTTGAAGGAA ATGACACACT CATGGCCACC TCTCTTGACA 
GCTATTCACA CGCCAAGTGC TGCAGAGCCC TCCAAGTTTC CCTTTCCAAC AAAGGAATCG 
CAGCATATCC CATCTGTAGC ACAGAATCAC AAGCAGTATG ACGCACCTTC CAAAACGTTG 
CCTAGTTCTC ATCCAACAAT ATCAGTGCTC CAGGAGGACC TGCAGCTCAG TGATAGTGAA 
GAGAGTGGTG ACGACCAAGT TGTTGAACAG CCACCTTCTT CACTTGATCC TCCAAGTGCC 
CTGCAGTCCC AGCCTGAGAG CGTGGCATCA GCACATTCCA ACAGCACAGA GTCAGGGAGC 
AGCAGTGACT CAGACAGCTC TTCAGACTCT TCAGAGACAG AGAGCAGCTC GAGTGACAGT 
GAAGCCAACG ACCCTCCAAG AGCGTCAGCT CCTGAGCCCG ACCCACCAAC TTCCAATAAG 
TGGCAGCTTG ACAACTGGCT CACCAAGGTG AACCCACCAG CAGTGCCAAC AGAAAGCCCC 
AGCGACACTG CCCACACAGA TGGCCACGAG GAGGACAAGG AACAACCCGT CAGCAACGCT 
TCCTCCTGCG AGCATGCTGT GCCAAGGGAA CCTCACGAGA GAAGCTCCAT CCGAGCAGCC 
CAGGCTCCTC AGGATGTTCA TCTTCCGACC AAACTTAGCT GCCAGAAGTC TCCTGTGCGC 
GCTGAGGAGC CCTCACAGAG GCAGACTGTG GGGATCAAAA GGCCCAGCAA GCCTCCAGTG 
CACGAGGAAC CCAAGGGAGG CCTGAAGGTG GAGAGCGAGC CCGCTCCTTA TGAGATTGGA 
GAGCAGTCTT CCAGAGACAA GCCAAAACCC AAAACAAAAA GCAGATCCAA ATCCTGTGAC 
CAAAAAGAAC TGAAACCCCG GCTGCAGGAG GCTCCTAAAG AGAAGAAGCA CAAGAGCTCC 
CACCACGCCG ATGCCAATGC CCTGCCGGAC CACAGACCCG TGAGTGATGT TCTGGTAGGC 
AGTGCCCAGG AGCCTTCCAC TCTCAAACAC CTCCCTGAGG GCCAGGGCAC AGCTCCCACC 
AGGACTAACG GCCACAGGTC TGCTGCTGAG GTCAAGGAGG GTTTTCACAA GGGGAAGCTT 
CCTGTGCCCA CCAAGGAGAA GAAGTTGCTG CCACCGGTGA GGGACGTGCC TGGCAATCCC 
AGCCTCCTGG TTAGAATTGA TCTCCCACTC CTGTCAAGAG TTCCCCAGCC TCCTGAGAAG 
GGGAACCAAA AGAAGAGAGC AGAGGTGAAG GAGCACCCTG GCACCAAGGA GCAGGACTTG 
GAGAAGAAAA GCACAGACAC TCCTGACAGG TCCTTCAAAA AGCGAAAGAG AGAAAAGGAG 
AAGGACGTCG ATAGGAAGAA AAGGAAGTTG GGCAAAGAGG CAAAATCATT GCAGTCTTCA 
CCTAACAAAG ACTCCTGTAA ATTGAAAGCA CGGAACGCTT CGCCTGAGAC CCAGAACAAA 
GATCTCAGGC AACCCCTGCC ACTGCCGCCC ATGTCTCCCA CGCATCCTGC ACCAAAGCCA 
ACCAAGAAGG CCCACAAGAG GCCCAGGAGT GAGAACGGCC AGCCGCCTGC CACTGCCAGC 
AGCACTGCCC TGGATGCAAG CAACCACAAG GATCCTTCTT CCACCAAGCA GAAGAAGATG 
GTGGAAGACC ACTCTGAGCA GGGATCTGCA GGGGATGTCA CAAATCCTTT ACCAGTGCCT 
TCTGTGCCAA ATGGTACCTC CAAACCAAGG AGACCTCAGA TCAAGTTTGA AAGACAGCAT 
TTGTCAGAAT ATTACCTGGA AGAGGCCAAG AAGATGAAGC ACGAGGCTGA TGCCATGATG 
GACAAGACTG GAAAGGCCTT TCAGTACCTC GATGCTGCCC TTTCCTTTGT TGAATACGGG 
ATGGCCCTGG AATCGAATGC GGTAGCACCA AAACCTGCTT ACAGCATCTT TGCTGACACA 
ATAGATCTCC TCAAATACGT AATGACACTA AAACCCTTTA CGGATGTCTC GGCATCTTCA 
CATGAACACG TCTTTGCTGT GTTATGCATG CGCTGCCAGT CCCTCCTGCA CATGGCAATG 
TTTCGTTACA AAAGAGACAC TGCAATAAAG TATTCCAGGA TCCTGAATGA CCACTTCAAG 
AGTTCTTCCA GAGTAACACA GGCACCTTCT CCATGTGTTG CAAGAAGCAC AGGCATGCCT 
TCTCCTCATT CTCCAGTGCC CTCTCCTGCC AGATCTATGA GTTCTCAGCA GGGATCAAAT 
GGTAGCAATT GTGGTTGCAA CAGCATTGGC TCTTCAGTCA CCATCCCAAG CATCACCTCT 
TCATACGTCA ACATCACTTC CTATGTCCTC TATGCGTATA ATATTTGGGA ACATGCTGAT 
GCACTGTCCA AGAATAATAA GGAGTTTTTT GCTGAACTCA GCACAGCGGT GTGCCCTCTG 
GCACTGACCA GTAGCATGGC CGAAATGGTA CACTACACTA GACAGGGTTT ACATTGGCTG 
AGACTGAACA CAAGTACGCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_420549.4
CDS	4..3747
Translation	MMKTMAESCQRKVWLGERLVLKEVVKKPGLESPLQPEMGLRPKGASAAFPGSLYNEDRNLLRLQERQRRNQEALQEKDKFLENTPLFPEPYKTNKEDELSSRIQNMLGNYDDLQDLINDRCHQNLIGIPESDVPQFPQRKPDRPSFPEKTSRTLSSSFHHATRYPHMGPPVIAPPRPGHYAPHQKAQPGTGPAPGWHAKSHSSSSSWSQGQEHKRGRPDSHAGSHHNRTDRHPAGGGRAPEPQATLLELSPLLPSLSSPVAPLSPLHSIQHTNSKSQKSSKGHGPAYTQSPQDRAVGSRENKSQDNSAVILAGTTQPSSQPFPPPLPSKTSAMQQKPTAYVRPMDGQEQAPVESPDLKPLLEEYHGEPYENISDLKANTKAKLSKLNISSEPTEQTLPSDSQCVEEILKEMTHSWPPLLTAIHTPSAAEPSKFPFPTKESQHIPSVAQNHKQYDAPSKTLPSSHPTISVLQEDLQLSDSEESGDDQVVEQPPSSLDPPSALQSQPESVASAHSNSTESGSSSDSDSSSDSSETESSSSDSEANDPPRASAPEPDPPTSNKWQLDNWLTKVNPPAVPTESPSDTAHTDGHEEDKEQPVSNASSCEHAVPREPHERSSIRAAQAPQDVHLPTKLSCQKSPVRAEEPSQRQTVGIKRPSKPPVHEEPKGGLKVESEPAPYEIGEQSSRDKPKPKTKSRSKSCDQKELKPRLQEAPKEKKHKSSHHADANALPDHRPVSDVLVGSAQEPSTLKHLPEGQGTAPTRTNGHRSAAEVKEGFHKGKLPVPTKEKKLLPPVRDVPGNPSLLVRIDLPLLSRVPQPPEKGNQKKRAEVKEHPGTKEQDLEKKSTDTPDRSFKKRKREKEKDVDRKKRKLGKEAKSLQSSPNKDSCKLKARNASPETQNKDLRQPLPLPPMSPTHPAPKPTKKAHKRPRSENGQPPATASSTALDASNHKDPSSTKQKKMVEDHSEQGSAGDVTNPLPVPSVPNGTSKPRRPQIKFERQHLSEYYLEEAKKMKHEADAMMDKTGKAFQYLDAALSFVEYGMALESNAVAPKPAYSIFADTIDLLKYVMTLKPFTDVSASSHEHVFAVLCMRCQSLLHMAMFRYKRDTAIKYSRILNDHFKSSSRVTQAPSPCVARSTGMPSPHSPVPSPARSMSSQQGSNGSNCGCNSIGSSVTIPSITSSYVNITSYVLYAYNIWEHADALSKNNKEFFAELSTAVCPLALTSSMAEMVHYTRQGLHWLRLNTSTP

Target ORF information:

RefSeq Version	XM_420549.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_420549.6

ORF Insert Sequence:

ATGATGAAAA CGATGGCTGA GAGCTGCCAG AGGAAAGTTT GGCTGGGAGA ACGGCTGGTG 
CTAAAGGAGG TGGTGAAGAA ACCTGGTTTA GAAAGTCCAT TGCAGCCTGA GATGGGATTA 
CGTCCAAAAG GTGCCTCTGC TGCTTTCCCT GGGAGCTTGT ACAATGAGGA CAGAAACTTG 
CTGCGTCTTC AGGAGAGACA GAGGCGGAAT CAGGAGGCTC TGCAGGAGAA AGACAAGTTC 
CTTGAGAACA CCCCACTCTT TCCTGAGCCG TACAAGACTA ATAAAGAGGA TGAGTTGTCA 
AGCCGTATTC AGAATATGCT GGGCAATTAC GACGACTTGC AGGATCTTAT TAATGACAGA 
TGCCATCAGA ATCTCATAGG GATACCAGAG AGCGACGTTC CTCAGTTCCC ACAAAGAAAG 
CCCGACCGCC CATCCTTCCC TGAAAAGACC AGCCGTACGT TATCGTCTTC ATTCCACCAT 
GCAACCCGCT ATCCACACAT GGGACCCCCG GTCATTGCTC CTCCCCGTCC AGGCCACTAC 
GCTCCCCACC AAAAGGCACA ACCAGGAACA GGACCAGCTC CAGGCTGGCA TGCCAAGAGT 
CACAGCTCGT CCAGCAGCTG GAGCCAAGGT CAGGAACACA AGCGTGGCAG GCCGGACAGT 
CATGCCGGTT CTCACCACAA CAGAACTGAC AGGCATCCTG CTGGTGGAGG TCGTGCTCCT 
GAACCTCAGG CCACTCTTTT GGAGCTTTCC CCGTTGCTGC CGTCTTTGTC TTCTCCAGTG 
GCACCCCTGT CACCTCTACA TTCCATCCAG CACACTAATT CCAAGTCACA GAAGAGCAGC 
AAAGGTCATG GGCCGGCCTA CACTCAATCA CCTCAGGACC GAGCGGTGGG TTCACGGGAG 
AATAAAAGTC AGGACAACTC AGCCGTTATC TTGGCTGGCA CCACTCAGCC TTCCTCTCAA 
CCCTTTCCTC CACCTTTGCC ATCAAAAACC AGTGCAATGC AGCAGAAACC AACTGCCTAC 
GTCAGACCAA TGGATGGCCA AGAGCAGGCT CCGGTGGAGT CACCGGATCT CAAGCCTCTA 
CTGGAGGAAT ACCACGGTGA ACCGTATGAA AACATCTCAG ATTTGAAGGC AAACACCAAG 
GCCAAATTAT CCAAATTGAA CATCTCTTCC GAGCCCACTG AGCAAACCTT GCCCAGTGAT 
AGCCAGTGCG TTGAAGAAAT TTTGAAGGAA ATGACACACT CATGGCCACC TCTCTTGACA 
GCTATTCACA CGCCAAGTGC TGCAGAGCCC TCCAAGTTTC CCTTTCCAAC AAAGGAATCG 
CAGCATATCC CATCTGTAGC ACAGAATCAC AAGCAGTATG ACGCACCTTC CAAAACGTTG 
CCTAGTTCTC ATCCAACAAT ATCAGTGCTC CAGGAGGACC TGCAGCTCAG TGATAGTGAA 
GAGAGTGGTG ACGACCAAGT TGTTGAACAG CCACCTTCTT CACTTGATCC TCCAAGTGCC 
CTGCAGTCCC AGCCTGAGAG CGTGGCATCA GCACATTCCA ACAGCACAGA GTCAGGGAGC 
AGCAGTGACT CAGACAGCTC TTCAGACTCT TCAGAGACAG AGAGCAGCTC GAGTGACAGT 
GAAGCCAACG ACCCTCCAAG AGCGTCAGCT CCTGAGCCCG ACCCACCAAC TTCCAATAAG 
TGGCAGCTTG ACAACTGGCT CACCAAGGTG AACCCACCAG CAGTGCCAAC AGAAAGCCCC 
AGCGACACTG CCCACACAGA TGGCCACGAG GAGGACAAGG AACAACCCGT CAGCAACGCT 
TCCTCCTGCG AGCATGCTGT GCCAAGGGAA CCTCACGAGA GAAGCTCCAT CCGAGCAGCC 
CAGGCTCCTC AGGATGTTCA TCTTCCGACC AAACTTAGCT GCCAGAAGTC TCCTGTGCGC 
GCTGAGGAGC CCTCACAGAG GCAGACTGTG GGGATCAAAA GGCCCAGCAA GCCTCCAGTG 
CACGAGGAAC CCAAGGGAGG CCTGAAGGTG GAGAGCGAGC CCGCTCCTTA TGAGATTGGA 
GAGCAGTCTT CCAGAGACAA GCCAAAACCC AAAACAAAAA GCAGATCCAA ATCCTGTGAC 
CAAAAAGAAC TGAAACCCCG GCTGCAGGAG GCTCCTAAAG AGAAGAAGCA CAAGAGCTCC 
CACCACGCCG ATGCCAATGC CCTGCCGGAC CACAGACCCG TGAGTGATGT TCTGGTAGGC 
AGTGCCCAGG AGCCTTCCAC TCTCAAACAC CTCCCTGAGG GCCAGGGCAC AGCTCCCACC 
AGGACTAACG GCCACAGGTC TGCTGCTGAG GTCAAGGAGG GTTTTCACAA GGGGAAGCTT 
CCTGTGCCCA CCAAGGAGAA GAAGTTGCTG CCACCGGTGA GGGACGTGCC TGGCAATCCC 
AGCCTCCTGG TTAGAATTGA TCTCCCACTC CTGTCAAGAG TTCCCCAGCC TCCTGAGAAG 
GGGAACCAAA AGAAGAGAGC AGAGGTGAAG GAGCACCCTG GCACCAAGGA GCAGGACTTG 
GAGAAGAAAA GCACAGACAC TCCTGACAGG TCCTTCAAAA AGCGAAAGAG AGAAAAGGAG 
AAGGACGTCG ATAGGAAGAA AAGGAAGTTG GGCAAAGAGG CAAAATCATT GCAGTCTTCA 
CCTAACAAAG ACTCCTGTAA ATTGAAAGCA CGGAACGCTT CGCCTGAGAC CCAGAACAAA 
GATCTCAGGC AACCCCTGCC ACTGCCGCCC ATGTCTCCCA CGCATCCTGC ACCAAAGCCA 
ACCAAGAAGG CCCACAAGAG GCCCAGGAGT GAGAACGGCC AGCCGCCTGC CACTGCCAGC 
AGCACTGCCC TGGATGCAAG CAACCACAAG GATCCTTCTT CCACCAAGCA GAAGAAGATG 
GTGGAAGACC ACTCTGAGCA GGGATCTGCA GGGGATGTCA CAAATCCTTT ACCAGTGCCT 
TCTGTGCCAA ATGGTACCTC CAAACCAAGG AGACCTCAGA TCAAGTTTGA AAGACAGCAT 
TTGTCAGAAT ATTACCTGGA AGAGGCCAAG AAGATGAAGC ACGAGGCTGA TGCCATGATG 
GACAAGACTG GAAAGGCCTT TCAGTACCTC GATGCTGCCC TTTCCTTTGT TGAATACGGG 
ATGGCCCTGG AATCGAATGC GGTAGCACCA AAACCTGCTT ACAGCATCTT TGCTGACACA 
ATAGATCTCC TCAAATACGT AATGACACTA AAACCCTTTA CGGATGTCTC GGCATCTTCA 
CATGAACACG TCTTTGCTGT GTTATGCATG CGCTGCCAGT CCCTCCTGCA CATGGCAATG 
TTTCGTTACA AAAGAGACAC TGCAATAAAG TATTCCAGGA TCCTGAATGA CCACTTCAAG 
AGTTCTTCCA GAGTAACACA GGCACCTTCT CCATGTGTTG CAAGAAGCAC AGGCATGCCT 
TCTCCTCATT CTCCAGTGCC CTCTCCTGCC AGATCTATGA GTTCTCAGCA GGGATCAAAT 
GGTAGCAATT GTGGTTGCAA CAGCATTGGC TCTTCAGTCA CCATCCCAAG CATCACCTCT 
TCATACGTCA ACATCACTTC CTATGTCCTC TATGCGTATA ATATTTGGGA ACATGCTGAT 
GCACTGTCCA AGAATAATAA GGAGTTTTTT GCTGAACTCA GCACAGCGGT GTGCCCTCTG 
GCACTGACCA GTAGCATGGC CGAAATGGTA CACTACACTA GACAGGGTTT ACATTGGCTG 
AGACTGAACA CAAGTACGCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_004941099.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004941156.1 Gallus gallus AF4/FMR2 family, member 1 (AFF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa08003
Clone ID Related Accession (Same CDS sequence)	XM_004941099.2 , XM_025150377.1
Accession Version	XM_004941099.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	AF4/FMR2 family member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004941099.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTC CAGGCAAAGA GAGCATCCCA CGTGATCGCT TGTACAATGA GGACAGAAAC 
TTGCTGCGTC TTCAGGAGAG ACAGAGGCGG AATCAGGAGG CTCTGCAGGA GAAAGACAAG 
TTCCTTGAGA ACACCCCACT CTTTCCTGAG CCGTACAAGA CTAATAAAGA GGATGAGTTG 
TCAAGCCGTA TTCAGAATAT GCTGGGCAAT TACGACGACT TGCAGGATCT TATTAATGAC 
AGATGCCATC AGAATCTCAT AGGGATACCA GAGAGCGACG TTCCTCAGTT CCCACAAAGA 
AAGCCCGACC GCCCATCCTT CCCTGAAAAG ACCAGCCGTA CGTTATCGTC TTCATTCCAC 
CATGCAACCC GCTATCCACA CATGGGACCC CCGGTCATTG CTCCTCCCCG TCCAGGCCAC 
TACGCTCCCC ACCAAAAGGC ACAACCAGGA ACAGGACCAG CTCCAGGCTG GCATGCCAAG 
AGTCACAGCT CGTCCAGCAG CTGGAGCCAA GGTCAGGAAC ACAAGCGTGG CAGGCCGGAC 
AGTCATGCCG GTTCTCACCA CAACAGAACT GACAGGCATC CTGCTGGTGG AGGTCGTGCT 
CCTGAACCTC AGGCCACTCT TTTGGAGCTT TCCCCGTTGC TGCCGTCTTT GTCTTCTCCA 
GTGGCACCCC TGTCACCTCT ACATTCCATC CAGCACACTA ATTCCAAGTC ACAGAAGAGC 
AGCAAAGGTC ATGGGCCGGC CTACACTCAA TCACCTCAGG ACCGAGCGGT GGGTTCACGG 
GAGAATAAAA GTCAGGACAA CTCAGCCGTT ATCTTGGCTG GCACCACTCA GCCTTCCTCT 
CAACCCTTTC CTCCACCTTT GCCATCAAAA ACCAGTGCAA TGCAGCAGAA ACCAACTGCC 
TACGTCAGAC CAATGGATGG CCAAGAGCAG GCTCCGGTGG AGTCACCGGA TCTCAAGCCT 
CTACTGGAGG AATACCACGG TGAACCGTAT GAAAACATCT CAGATTTGAA GGCAAACACC 
AAGGCCAAAT TATCCAAATT GAACATCTCT TCCGAGCCCA CTGAGCAAAC CTTGCCCAGT 
GATAGCCAGT GCGTTGAAGA AATTTTGAAG GAAATGACAC ACTCATGGCC ACCTCTCTTG 
ACAGCTATTC ACACGCCAAG TGCTGCAGAG CCCTCCAAGT TTCCCTTTCC AACAAAGGAA 
TCGCAGCATA TCCCATCTGT AGCACAGAAT CACAAGCAGT ATGACGCACC TTCCAAAACG 
TTGCCTAGTT CTCATCCAAC AATATCAGTG CTCCAGGAGG ACCTGCAGCT CAGTGATAGT 
GAAGAGAGTG GTGACGACCA AGTTGTTGAA CAGCCACCTT CTTCACTTGA TCCTCCAAGT 
GCCCTGCAGT CCCAGCCTGA GAGCGTGGCA TCAGCACATT CCAACAGCAC AGAGTCAGGG 
AGCAGCAGTG ACTCAGACAG CTCTTCAGAC TCTTCAGAGA CAGAGAGCAG CTCGAGTGAC 
AGTGAAGCCA ACGACCCTCC AAGAGCGTCA GCTCCTGAGC CCGACCCACC AACTTCCAAT 
AAGTGGCAGC TTGACAACTG GCTCACCAAG GTGAACCCAC CAGCAGTGCC AACAGAAAGC 
CCCAGCGACA CTGCCCACAC AGATGGCCAC GAGGAGGACA AGGAACAACC CGTCAGCAAC 
GCTTCCTCCT GCGAGCATGC TGTGCCAAGG GAACCTCACG AGAGAAGCTC CATCCGAGCA 
GCCCAGGCTC CTCAGGATGT TCATCTTCCG ACCAAACTTA GCTGCCAGAA GTCTCCTGTG 
CGCGCTGAGG AGCCCTCACA GAGGCAGACT GTGGGGATCA AAAGGCCCAG CAAGCCTCCA 
GTGCACGAGG AACCCAAGGG AGGCCTGAAG GTGGAGAGCG AGCCCGCTCC TTATGAGATT 
GGAGAGCAGT CTTCCAGAGA CAAGCCAAAA CCCAAAACAA AAAGCAGATC CAAATCCTGT 
GACCAAAAAG AACTGAAACC CCGGCTGCAG GAGGCTCCTA AAGAGAAGAA GCACAAGAGC 
TCCCACCACG CCGATGCCAA TGCCCTGCCG GACCACAGAC CCGTGAGTGA TGTTCTGGTA 
GGCAGTGCCC AGGAGCCTTC CACTCTCAAA CACCTCCCTG AGGGCCAGGG CACAGCTCCC 
ACCAGGACTA ACGGCCACAG GTCTGCTGCT GAGGTCAAGG AGGGTTTTCA CAAGGGGAAG 
CTTCCTGTGC CCACCAAGGA GAAGAAGTTG CTGCCACCGG TGAGGGACGT GCCTGGCAAT 
CCCAGCCTCC TGGTTAGAAT TGATCTCCCA CTCCTGTCAA GAGTTCCCCA GCCTCCTGAG 
AAGGGGAACC AAAAGAAGAG AGCAGAGGTG AAGGAGCACC CTGGCACCAA GGAGCAGGAC 
TTGGAGAAGA AAAGCACAGA CACTCCTGAC AGGTCCTTCA AAAAGCGAAA GAGAGAAAAG 
GAGAAGGACG TCGATAGGAA GAAAAGGAAG TTGGGCAAAG AGGCAAAATC ATTGCAGTCT 
TCACCTAACA AAGACTCCTG TAAATTGAAA GCACGGAACG CTTCGCCTGA GACCCAGAAC 
AAAGATCTCA GGCAACCCCT GCCACTGCCG CCCATGTCTC CCACGCATCC TGCACCAAAG 
CCAACCAAGA AGGCCCACAA GAGGCCCAGG AGTGAGAACG GCCAGCCGCC TGCCACTGCC 
AGCAGCACTG CCCTGGATGC AAGCAACCAC AAGGATCCTT CTTCCACCAA GCAGAAGAAG 
ATGGTGGAAG ACCACTCTGA GCAGGTGAAG GGCAACAAAG GATCTGCAGG GGATGTCACA 
AATCCTTTAC CAGTGCCTTC TGTGCCAAAT GGTACCTCCA AACCAAGGAG ACCTCAGATC 
AAGTTTGAAA GACAGCATTT GTCAGAATAT TACCTGGAAG AGGCCAAGAA GATGAAGCAC 
GAGGCTGATG CCATGATGGA CAAGACTGGA AAGGCCTTTC AGTACCTCGA TGCTGCCCTT 
TCCTTTGTTG AATACGGGAT GGCCCTGGAA TCGAATGCGG TAGCACCAAA ACCTGCTTAC 
AGCATCTTTG CTGACACAAT AGATCTCCTC AAATACGTAA TGACACTAAA ACCCTTTACG 
GATGTCTCGG CATCTTCACA TGAACACGTC TTTGCTGTGT TATGCATGCG CTGCCAGTCC 
CTCCTGCACA TGGCAATGTT TCGTTACAAA AGAGACACTG CAATAAAGTA TTCCAGGATC 
CTGAATGACC ACTTCAAGAG TTCTTCCAGA GTAACACAGG CACCTTCTCC ATGTGTTGCA 
AGAAGCACAG GCATGCCTTC TCCTCATTCT CCAGTGCCCT CTCCTGCCAG ATCTATGAGT 
TCTCAGCAGG GATCAAATGG TAGCAATTGT GGTTGCAACA GCATTGGCTC TTCAGTCACC 
ATCCCAAGCA TCACCTCTTC ATACGTCAAC ATCACTTCCT ATGTCCTCTA TGCGTATAAT 
ATTTGGGAAC ATGCTGATGC ACTGTCCAAG AATAATAAGG AGTTTTTTGC TGAACTCAGC 
ACAGCGGTGT GCCCTCTGGC ACTGACCAGT AGCATGGCCG AAATGGTACA CTACACTAGA 
CAGGGTTTAC ATTGGCTGAG ACTGAACACA AGTACGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004941156.1
CDS	312..3953
Translation	MASPGKESIPRDRLYNEDRNLLRLQERQRRNQEALQEKDKFLENTPLFPEPYKTNKEDELSSRIQNMLGNYDDLQDLINDRCHQNLIGIPESDVPQFPQRKPDRPSFPEKTSRTLSSSFHHATRYPHMGPPVIAPPRPGHYAPHQKAQPGTGPAPGWHAKSHSSSSSWSQGQEHKRGRPDSHAGSHHNRTDRHPAGGGRAPEPQATLLELSPLLPSLSSPVAPLSPLHSIQHTNSKSQKSSKGHGPAYTQSPQDRAVGSRENKSQDNSAVILAGTTQPSSQPFPPPLPSKTSAMQQKPTAYVRPMDGQEQAPVESPDLKPLLEEYHGEPYENISDLKANTKAKLSKLNISSEPTEQTLPSDSQCVEEILKEMTHSWPPLLTAIHTPSAAEPSKFPFPTKESQHIPSVAQNHKQYDAPSKTLPSSHPTISVLQEDLQLSDSEESGDDQVVEQPPSSLDPPSALQSQPESVASAHSNSTESGSSSDSDSSSDSSETESSSSDSEANDPPRASAPEPDPPTSNKWQLDNWLTKVNPPAVPTESPSDTAHTDGHEEDKEQPVSNASSCEHAVPREPHERSSIRAAQAPQDVHLPTKLSCQKSPVRAEEPSQRQTVGIKRPSKPPVHEEPKGGLKVESEPAPYEIGEQSSRDKPKPKTKSRSKSCDQKELKPRLQEAPKEKKHKSSHHADANALPDHRPVSDVLVGSAQEPSTLKHLPEGQGTAPTRTNGHRSAAEVKEGFHKGKLPVPTKEKKLLPPVRDVPGNPSLLVRIDLPLLSRVPQPPEKGNQKKRAEVKEHPGTKEQDLEKKSTDTPDRSFKKRKREKEKDVDRKKRKLGKEAKSLQSSPNKDSCKLKARNASPETQNKDLRQPLPLPPMSPTHPAPKPTKKAHKRPRSENGQPPATASSTALDASNHKDPSSTKQKKMVEDHSEQVKGNKGSAGDVTNPLPVPSVPNGTSKPRRPQIKFERQHLSEYYLEEAKKMKHEADAMMDKTGKAFQYLDAALSFVEYGMALESNAVAPKPAYSIFADTIDLLKYVMTLKPFTDVSASSHEHVFAVLCMRCQSLLHMAMFRYKRDTAIKYSRILNDHFKSSSRVTQAPSPCVARSTGMPSPHSPVPSPARSMSSQQGSNGSNCGCNSIGSSVTIPSITSSYVNITSYVLYAYNIWEHADALSKNNKEFFAELSTAVCPLALTSSMAEMVHYTRQGLHWLRLNTSTP

Target ORF information:

RefSeq Version	XM_004941099.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus AF4/FMR2 family, member 1 (AFF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004941099.2

ORF Insert Sequence:

ATGGCATCTC CAGGCAAAGA GAGCATCCCA CGTGATCGCT TGTACAATGA GGACAGAAAC 
TTGCTGCGTC TTCAGGAGAG ACAGAGGCGG AATCAGGAGG CTCTGCAGGA GAAAGACAAG 
TTCCTTGAGA ACACCCCACT CTTTCCTGAG CCGTACAAGA CTAATAAAGA GGATGAGTTG 
TCAAGCCGTA TTCAGAATAT GCTGGGCAAT TACGACGACT TGCAGGATCT TATTAATGAC 
AGATGCCATC AGAATCTCAT AGGGATACCA GAGAGCGACG TTCCTCAGTT CCCACAAAGA 
AAGCCCGACC GCCCATCCTT CCCTGAAAAG ACCAGCCGTA CGTTATCGTC TTCATTCCAC 
CATGCAACCC GCTATCCACA CATGGGACCC CCGGTCATTG CTCCTCCCCG TCCAGGCCAC 
TACGCTCCCC ACCAAAAGGC ACAACCAGGA ACAGGACCAG CTCCAGGCTG GCATGCCAAG 
AGTCACAGCT CGTCCAGCAG CTGGAGCCAA GGTCAGGAAC ACAAGCGTGG CAGGCCGGAC 
AGTCATGCCG GTTCTCACCA CAACAGAACT GACAGGCATC CTGCTGGTGG AGGTCGTGCT 
CCTGAACCTC AGGCCACTCT TTTGGAGCTT TCCCCGTTGC TGCCGTCTTT GTCTTCTCCA 
GTGGCACCCC TGTCACCTCT ACATTCCATC CAGCACACTA ATTCCAAGTC ACAGAAGAGC 
AGCAAAGGTC ATGGGCCGGC CTACACTCAA TCACCTCAGG ACCGAGCGGT GGGTTCACGG 
GAGAATAAAA GTCAGGACAA CTCAGCCGTT ATCTTGGCTG GCACCACTCA GCCTTCCTCT 
CAACCCTTTC CTCCACCTTT GCCATCAAAA ACCAGTGCAA TGCAGCAGAA ACCAACTGCC 
TACGTCAGAC CAATGGATGG CCAAGAGCAG GCTCCGGTGG AGTCACCGGA TCTCAAGCCT 
CTACTGGAGG AATACCACGG TGAACCGTAT GAAAACATCT CAGATTTGAA GGCAAACACC 
AAGGCCAAAT TATCCAAATT GAACATCTCT TCCGAGCCCA CTGAGCAAAC CTTGCCCAGT 
GATAGCCAGT GCGTTGAAGA AATTTTGAAG GAAATGACAC ACTCATGGCC ACCTCTCTTG 
ACAGCTATTC ACACGCCAAG TGCTGCAGAG CCCTCCAAGT TTCCCTTTCC AACAAAGGAA 
TCGCAGCATA TCCCATCTGT AGCACAGAAT CACAAGCAGT ATGACGCACC TTCCAAAACG 
TTGCCTAGTT CTCATCCAAC AATATCAGTG CTCCAGGAGG ACCTGCAGCT CAGTGATAGT 
GAAGAGAGTG GTGACGACCA AGTTGTTGAA CAGCCACCTT CTTCACTTGA TCCTCCAAGT 
GCCCTGCAGT CCCAGCCTGA GAGCGTGGCA TCAGCACATT CCAACAGCAC AGAGTCAGGG 
AGCAGCAGTG ACTCAGACAG CTCTTCAGAC TCTTCAGAGA CAGAGAGCAG CTCGAGTGAC 
AGTGAAGCCA ACGACCCTCC AAGAGCGTCA GCTCCTGAGC CCGACCCACC AACTTCCAAT 
AAGTGGCAGC TTGACAACTG GCTCACCAAG GTGAACCCAC CAGCAGTGCC AACAGAAAGC 
CCCAGCGACA CTGCCCACAC AGATGGCCAC GAGGAGGACA AGGAACAACC CGTCAGCAAC 
GCTTCCTCCT GCGAGCATGC TGTGCCAAGG GAACCTCACG AGAGAAGCTC CATCCGAGCA 
GCCCAGGCTC CTCAGGATGT TCATCTTCCG ACCAAACTTA GCTGCCAGAA GTCTCCTGTG 
CGCGCTGAGG AGCCCTCACA GAGGCAGACT GTGGGGATCA AAAGGCCCAG CAAGCCTCCA 
GTGCACGAGG AACCCAAGGG AGGCCTGAAG GTGGAGAGCG AGCCCGCTCC TTATGAGATT 
GGAGAGCAGT CTTCCAGAGA CAAGCCAAAA CCCAAAACAA AAAGCAGATC CAAATCCTGT 
GACCAAAAAG AACTGAAACC CCGGCTGCAG GAGGCTCCTA AAGAGAAGAA GCACAAGAGC 
TCCCACCACG CCGATGCCAA TGCCCTGCCG GACCACAGAC CCGTGAGTGA TGTTCTGGTA 
GGCAGTGCCC AGGAGCCTTC CACTCTCAAA CACCTCCCTG AGGGCCAGGG CACAGCTCCC 
ACCAGGACTA ACGGCCACAG GTCTGCTGCT GAGGTCAAGG AGGGTTTTCA CAAGGGGAAG 
CTTCCTGTGC CCACCAAGGA GAAGAAGTTG CTGCCACCGG TGAGGGACGT GCCTGGCAAT 
CCCAGCCTCC TGGTTAGAAT TGATCTCCCA CTCCTGTCAA GAGTTCCCCA GCCTCCTGAG 
AAGGGGAACC AAAAGAAGAG AGCAGAGGTG AAGGAGCACC CTGGCACCAA GGAGCAGGAC 
TTGGAGAAGA AAAGCACAGA CACTCCTGAC AGGTCCTTCA AAAAGCGAAA GAGAGAAAAG 
GAGAAGGACG TCGATAGGAA GAAAAGGAAG TTGGGCAAAG AGGCAAAATC ATTGCAGTCT 
TCACCTAACA AAGACTCCTG TAAATTGAAA GCACGGAACG CTTCGCCTGA GACCCAGAAC 
AAAGATCTCA GGCAACCCCT GCCACTGCCG CCCATGTCTC CCACGCATCC TGCACCAAAG 
CCAACCAAGA AGGCCCACAA GAGGCCCAGG AGTGAGAACG GCCAGCCGCC TGCCACTGCC 
AGCAGCACTG CCCTGGATGC AAGCAACCAC AAGGATCCTT CTTCCACCAA GCAGAAGAAG 
ATGGTGGAAG ACCACTCTGA GCAGGTGAAG GGCAACAAAG GATCTGCAGG GGATGTCACA 
AATCCTTTAC CAGTGCCTTC TGTGCCAAAT GGTACCTCCA AACCAAGGAG ACCTCAGATC 
AAGTTTGAAA GACAGCATTT GTCAGAATAT TACCTGGAAG AGGCCAAGAA GATGAAGCAC 
GAGGCTGATG CCATGATGGA CAAGACTGGA AAGGCCTTTC AGTACCTCGA TGCTGCCCTT 
TCCTTTGTTG AATACGGGAT GGCCCTGGAA TCGAATGCGG TAGCACCAAA ACCTGCTTAC 
AGCATCTTTG CTGACACAAT AGATCTCCTC AAATACGTAA TGACACTAAA ACCCTTTACG 
GATGTCTCGG CATCTTCACA TGAACACGTC TTTGCTGTGT TATGCATGCG CTGCCAGTCC 
CTCCTGCACA TGGCAATGTT TCGTTACAAA AGAGACACTG CAATAAAGTA TTCCAGGATC 
CTGAATGACC ACTTCAAGAG TTCTTCCAGA GTAACACAGG CACCTTCTCC ATGTGTTGCA 
AGAAGCACAG GCATGCCTTC TCCTCATTCT CCAGTGCCCT CTCCTGCCAG ATCTATGAGT 
TCTCAGCAGG GATCAAATGG TAGCAATTGT GGTTGCAACA GCATTGGCTC TTCAGTCACC 
ATCCCAAGCA TCACCTCTTC ATACGTCAAC ATCACTTCCT ATGTCCTCTA TGCGTATAAT 
ATTTGGGAAC ATGCTGATGC ACTGTCCAAG AATAATAAGG AGTTTTTTGC TGAACTCAGC 
ACAGCGGTGT GCCCTCTGGC ACTGACCAGT AGCATGGCCG AAATGGTACA CTACACTAGA 
CAGGGTTTAC ATTGGCTGAG ACTGAACACA AGTACGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_025150377.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025006145.1 Gallus gallus AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA.

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CloneID	OGa08003
Clone ID Related Accession (Same CDS sequence)	XM_004941099.2 , XM_025150377.1
Accession Version	XM_025150377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	AF4/FMR2 family member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTC CAGGCAAAGA GAGCATCCCA CGTGATCGCT TGTACAATGA GGACAGAAAC 
TTGCTGCGTC TTCAGGAGAG ACAGAGGCGG AATCAGGAGG CTCTGCAGGA GAAAGACAAG 
TTCCTTGAGA ACACCCCACT CTTTCCTGAG CCGTACAAGA CTAATAAAGA GGATGAGTTG 
TCAAGCCGTA TTCAGAATAT GCTGGGCAAT TACGACGACT TGCAGGATCT TATTAATGAC 
AGATGCCATC AGAATCTCAT AGGGATACCA GAGAGCGACG TTCCTCAGTT CCCACAAAGA 
AAGCCCGACC GCCCATCCTT CCCTGAAAAG ACCAGCCGTA CGTTATCGTC TTCATTCCAC 
CATGCAACCC GCTATCCACA CATGGGACCC CCGGTCATTG CTCCTCCCCG TCCAGGCCAC 
TACGCTCCCC ACCAAAAGGC ACAACCAGGA ACAGGACCAG CTCCAGGCTG GCATGCCAAG 
AGTCACAGCT CGTCCAGCAG CTGGAGCCAA GGTCAGGAAC ACAAGCGTGG CAGGCCGGAC 
AGTCATGCCG GTTCTCACCA CAACAGAACT GACAGGCATC CTGCTGGTGG AGGTCGTGCT 
CCTGAACCTC AGGCCACTCT TTTGGAGCTT TCCCCGTTGC TGCCGTCTTT GTCTTCTCCA 
GTGGCACCCC TGTCACCTCT ACATTCCATC CAGCACACTA ATTCCAAGTC ACAGAAGAGC 
AGCAAAGGTC ATGGGCCGGC CTACACTCAA TCACCTCAGG ACCGAGCGGT GGGTTCACGG 
GAGAATAAAA GTCAGGACAA CTCAGCCGTT ATCTTGGCTG GCACCACTCA GCCTTCCTCT 
CAACCCTTTC CTCCACCTTT GCCATCAAAA ACCAGTGCAA TGCAGCAGAA ACCAACTGCC 
TACGTCAGAC CAATGGATGG CCAAGAGCAG GCTCCGGTGG AGTCACCGGA TCTCAAGCCT 
CTACTGGAGG AATACCACGG TGAACCGTAT GAAAACATCT CAGATTTGAA GGCAAACACC 
AAGGCCAAAT TATCCAAATT GAACATCTCT TCCGAGCCCA CTGAGCAAAC CTTGCCCAGT 
GATAGCCAGT GCGTTGAAGA AATTTTGAAG GAAATGACAC ACTCATGGCC ACCTCTCTTG 
ACAGCTATTC ACACGCCAAG TGCTGCAGAG CCCTCCAAGT TTCCCTTTCC AACAAAGGAA 
TCGCAGCATA TCCCATCTGT AGCACAGAAT CACAAGCAGT ATGACGCACC TTCCAAAACG 
TTGCCTAGTT CTCATCCAAC AATATCAGTG CTCCAGGAGG ACCTGCAGCT CAGTGATAGT 
GAAGAGAGTG GTGACGACCA AGTTGTTGAA CAGCCACCTT CTTCACTTGA TCCTCCAAGT 
GCCCTGCAGT CCCAGCCTGA GAGCGTGGCA TCAGCACATT CCAACAGCAC AGAGTCAGGG 
AGCAGCAGTG ACTCAGACAG CTCTTCAGAC TCTTCAGAGA CAGAGAGCAG CTCGAGTGAC 
AGTGAAGCCA ACGACCCTCC AAGAGCGTCA GCTCCTGAGC CCGACCCACC AACTTCCAAT 
AAGTGGCAGC TTGACAACTG GCTCACCAAG GTGAACCCAC CAGCAGTGCC AACAGAAAGC 
CCCAGCGACA CTGCCCACAC AGATGGCCAC GAGGAGGACA AGGAACAACC CGTCAGCAAC 
GCTTCCTCCT GCGAGCATGC TGTGCCAAGG GAACCTCACG AGAGAAGCTC CATCCGAGCA 
GCCCAGGCTC CTCAGGATGT TCATCTTCCG ACCAAACTTA GCTGCCAGAA GTCTCCTGTG 
CGCGCTGAGG AGCCCTCACA GAGGCAGACT GTGGGGATCA AAAGGCCCAG CAAGCCTCCA 
GTGCACGAGG AACCCAAGGG AGGCCTGAAG GTGGAGAGCG AGCCCGCTCC TTATGAGATT 
GGAGAGCAGT CTTCCAGAGA CAAGCCAAAA CCCAAAACAA AAAGCAGATC CAAATCCTGT 
GACCAAAAAG AACTGAAACC CCGGCTGCAG GAGGCTCCTA AAGAGAAGAA GCACAAGAGC 
TCCCACCACG CCGATGCCAA TGCCCTGCCG GACCACAGAC CCGTGAGTGA TGTTCTGGTA 
GGCAGTGCCC AGGAGCCTTC CACTCTCAAA CACCTCCCTG AGGGCCAGGG CACAGCTCCC 
ACCAGGACTA ACGGCCACAG GTCTGCTGCT GAGGTCAAGG AGGGTTTTCA CAAGGGGAAG 
CTTCCTGTGC CCACCAAGGA GAAGAAGTTG CTGCCACCGG TGAGGGACGT GCCTGGCAAT 
CCCAGCCTCC TGGTTAGAAT TGATCTCCCA CTCCTGTCAA GAGTTCCCCA GCCTCCTGAG 
AAGGGGAACC AAAAGAAGAG AGCAGAGGTG AAGGAGCACC CTGGCACCAA GGAGCAGGAC 
TTGGAGAAGA AAAGCACAGA CACTCCTGAC AGGTCCTTCA AAAAGCGAAA GAGAGAAAAG 
GAGAAGGACG TCGATAGGAA GAAAAGGAAG TTGGGCAAAG AGGCAAAATC ATTGCAGTCT 
TCACCTAACA AAGACTCCTG TAAATTGAAA GCACGGAACG CTTCGCCTGA GACCCAGAAC 
AAAGATCTCA GGCAACCCCT GCCACTGCCG CCCATGTCTC CCACGCATCC TGCACCAAAG 
CCAACCAAGA AGGCCCACAA GAGGCCCAGG AGTGAGAACG GCCAGCCGCC TGCCACTGCC 
AGCAGCACTG CCCTGGATGC AAGCAACCAC AAGGATCCTT CTTCCACCAA GCAGAAGAAG 
ATGGTGGAAG ACCACTCTGA GCAGGTGAAG GGCAACAAAG GATCTGCAGG GGATGTCACA 
AATCCTTTAC CAGTGCCTTC TGTGCCAAAT GGTACCTCCA AACCAAGGAG ACCTCAGATC 
AAGTTTGAAA GACAGCATTT GTCAGAATAT TACCTGGAAG AGGCCAAGAA GATGAAGCAC 
GAGGCTGATG CCATGATGGA CAAGACTGGA AAGGCCTTTC AGTACCTCGA TGCTGCCCTT 
TCCTTTGTTG AATACGGGAT GGCCCTGGAA TCGAATGCGG TAGCACCAAA ACCTGCTTAC 
AGCATCTTTG CTGACACAAT AGATCTCCTC AAATACGTAA TGACACTAAA ACCCTTTACG 
GATGTCTCGG CATCTTCACA TGAACACGTC TTTGCTGTGT TATGCATGCG CTGCCAGTCC 
CTCCTGCACA TGGCAATGTT TCGTTACAAA AGAGACACTG CAATAAAGTA TTCCAGGATC 
CTGAATGACC ACTTCAAGAG TTCTTCCAGA GTAACACAGG CACCTTCTCC ATGTGTTGCA 
AGAAGCACAG GCATGCCTTC TCCTCATTCT CCAGTGCCCT CTCCTGCCAG ATCTATGAGT 
TCTCAGCAGG GATCAAATGG TAGCAATTGT GGTTGCAACA GCATTGGCTC TTCAGTCACC 
ATCCCAAGCA TCACCTCTTC ATACGTCAAC ATCACTTCCT ATGTCCTCTA TGCGTATAAT 
ATTTGGGAAC ATGCTGATGC ACTGTCCAAG AATAATAAGG AGTTTTTTGC TGAACTCAGC 
ACAGCGGTGT GCCCTCTGGC ACTGACCAGT AGCATGGCCG AAATGGTACA CTACACTAGA 
CAGGGTTTAC ATTGGCTGAG ACTGAACACA AGTACGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025006145.1
CDS	17..3658
Translation	MASPGKESIPRDRLYNEDRNLLRLQERQRRNQEALQEKDKFLENTPLFPEPYKTNKEDELSSRIQNMLGNYDDLQDLINDRCHQNLIGIPESDVPQFPQRKPDRPSFPEKTSRTLSSSFHHATRYPHMGPPVIAPPRPGHYAPHQKAQPGTGPAPGWHAKSHSSSSSWSQGQEHKRGRPDSHAGSHHNRTDRHPAGGGRAPEPQATLLELSPLLPSLSSPVAPLSPLHSIQHTNSKSQKSSKGHGPAYTQSPQDRAVGSRENKSQDNSAVILAGTTQPSSQPFPPPLPSKTSAMQQKPTAYVRPMDGQEQAPVESPDLKPLLEEYHGEPYENISDLKANTKAKLSKLNISSEPTEQTLPSDSQCVEEILKEMTHSWPPLLTAIHTPSAAEPSKFPFPTKESQHIPSVAQNHKQYDAPSKTLPSSHPTISVLQEDLQLSDSEESGDDQVVEQPPSSLDPPSALQSQPESVASAHSNSTESGSSSDSDSSSDSSETESSSSDSEANDPPRASAPEPDPPTSNKWQLDNWLTKVNPPAVPTESPSDTAHTDGHEEDKEQPVSNASSCEHAVPREPHERSSIRAAQAPQDVHLPTKLSCQKSPVRAEEPSQRQTVGIKRPSKPPVHEEPKGGLKVESEPAPYEIGEQSSRDKPKPKTKSRSKSCDQKELKPRLQEAPKEKKHKSSHHADANALPDHRPVSDVLVGSAQEPSTLKHLPEGQGTAPTRTNGHRSAAEVKEGFHKGKLPVPTKEKKLLPPVRDVPGNPSLLVRIDLPLLSRVPQPPEKGNQKKRAEVKEHPGTKEQDLEKKSTDTPDRSFKKRKREKEKDVDRKKRKLGKEAKSLQSSPNKDSCKLKARNASPETQNKDLRQPLPLPPMSPTHPAPKPTKKAHKRPRSENGQPPATASSTALDASNHKDPSSTKQKKMVEDHSEQVKGNKGSAGDVTNPLPVPSVPNGTSKPRRPQIKFERQHLSEYYLEEAKKMKHEADAMMDKTGKAFQYLDAALSFVEYGMALESNAVAPKPAYSIFADTIDLLKYVMTLKPFTDVSASSHEHVFAVLCMRCQSLLHMAMFRYKRDTAIKYSRILNDHFKSSSRVTQAPSPCVARSTGMPSPHSPVPSPARSMSSQQGSNGSNCGCNSIGSSVTIPSITSSYVNITSYVLYAYNIWEHADALSKNNKEFFAELSTAVCPLALTSSMAEMVHYTRQGLHWLRLNTSTP

Target ORF information:

RefSeq Version	XM_025150377.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150377.1

ORF Insert Sequence:

ATGGCATCTC CAGGCAAAGA GAGCATCCCA CGTGATCGCT TGTACAATGA GGACAGAAAC 
TTGCTGCGTC TTCAGGAGAG ACAGAGGCGG AATCAGGAGG CTCTGCAGGA GAAAGACAAG 
TTCCTTGAGA ACACCCCACT CTTTCCTGAG CCGTACAAGA CTAATAAAGA GGATGAGTTG 
TCAAGCCGTA TTCAGAATAT GCTGGGCAAT TACGACGACT TGCAGGATCT TATTAATGAC 
AGATGCCATC AGAATCTCAT AGGGATACCA GAGAGCGACG TTCCTCAGTT CCCACAAAGA 
AAGCCCGACC GCCCATCCTT CCCTGAAAAG ACCAGCCGTA CGTTATCGTC TTCATTCCAC 
CATGCAACCC GCTATCCACA CATGGGACCC CCGGTCATTG CTCCTCCCCG TCCAGGCCAC 
TACGCTCCCC ACCAAAAGGC ACAACCAGGA ACAGGACCAG CTCCAGGCTG GCATGCCAAG 
AGTCACAGCT CGTCCAGCAG CTGGAGCCAA GGTCAGGAAC ACAAGCGTGG CAGGCCGGAC 
AGTCATGCCG GTTCTCACCA CAACAGAACT GACAGGCATC CTGCTGGTGG AGGTCGTGCT 
CCTGAACCTC AGGCCACTCT TTTGGAGCTT TCCCCGTTGC TGCCGTCTTT GTCTTCTCCA 
GTGGCACCCC TGTCACCTCT ACATTCCATC CAGCACACTA ATTCCAAGTC ACAGAAGAGC 
AGCAAAGGTC ATGGGCCGGC CTACACTCAA TCACCTCAGG ACCGAGCGGT GGGTTCACGG 
GAGAATAAAA GTCAGGACAA CTCAGCCGTT ATCTTGGCTG GCACCACTCA GCCTTCCTCT 
CAACCCTTTC CTCCACCTTT GCCATCAAAA ACCAGTGCAA TGCAGCAGAA ACCAACTGCC 
TACGTCAGAC CAATGGATGG CCAAGAGCAG GCTCCGGTGG AGTCACCGGA TCTCAAGCCT 
CTACTGGAGG AATACCACGG TGAACCGTAT GAAAACATCT CAGATTTGAA GGCAAACACC 
AAGGCCAAAT TATCCAAATT GAACATCTCT TCCGAGCCCA CTGAGCAAAC CTTGCCCAGT 
GATAGCCAGT GCGTTGAAGA AATTTTGAAG GAAATGACAC ACTCATGGCC ACCTCTCTTG 
ACAGCTATTC ACACGCCAAG TGCTGCAGAG CCCTCCAAGT TTCCCTTTCC AACAAAGGAA 
TCGCAGCATA TCCCATCTGT AGCACAGAAT CACAAGCAGT ATGACGCACC TTCCAAAACG 
TTGCCTAGTT CTCATCCAAC AATATCAGTG CTCCAGGAGG ACCTGCAGCT CAGTGATAGT 
GAAGAGAGTG GTGACGACCA AGTTGTTGAA CAGCCACCTT CTTCACTTGA TCCTCCAAGT 
GCCCTGCAGT CCCAGCCTGA GAGCGTGGCA TCAGCACATT CCAACAGCAC AGAGTCAGGG 
AGCAGCAGTG ACTCAGACAG CTCTTCAGAC TCTTCAGAGA CAGAGAGCAG CTCGAGTGAC 
AGTGAAGCCA ACGACCCTCC AAGAGCGTCA GCTCCTGAGC CCGACCCACC AACTTCCAAT 
AAGTGGCAGC TTGACAACTG GCTCACCAAG GTGAACCCAC CAGCAGTGCC AACAGAAAGC 
CCCAGCGACA CTGCCCACAC AGATGGCCAC GAGGAGGACA AGGAACAACC CGTCAGCAAC 
GCTTCCTCCT GCGAGCATGC TGTGCCAAGG GAACCTCACG AGAGAAGCTC CATCCGAGCA 
GCCCAGGCTC CTCAGGATGT TCATCTTCCG ACCAAACTTA GCTGCCAGAA GTCTCCTGTG 
CGCGCTGAGG AGCCCTCACA GAGGCAGACT GTGGGGATCA AAAGGCCCAG CAAGCCTCCA 
GTGCACGAGG AACCCAAGGG AGGCCTGAAG GTGGAGAGCG AGCCCGCTCC TTATGAGATT 
GGAGAGCAGT CTTCCAGAGA CAAGCCAAAA CCCAAAACAA AAAGCAGATC CAAATCCTGT 
GACCAAAAAG AACTGAAACC CCGGCTGCAG GAGGCTCCTA AAGAGAAGAA GCACAAGAGC 
TCCCACCACG CCGATGCCAA TGCCCTGCCG GACCACAGAC CCGTGAGTGA TGTTCTGGTA 
GGCAGTGCCC AGGAGCCTTC CACTCTCAAA CACCTCCCTG AGGGCCAGGG CACAGCTCCC 
ACCAGGACTA ACGGCCACAG GTCTGCTGCT GAGGTCAAGG AGGGTTTTCA CAAGGGGAAG 
CTTCCTGTGC CCACCAAGGA GAAGAAGTTG CTGCCACCGG TGAGGGACGT GCCTGGCAAT 
CCCAGCCTCC TGGTTAGAAT TGATCTCCCA CTCCTGTCAA GAGTTCCCCA GCCTCCTGAG 
AAGGGGAACC AAAAGAAGAG AGCAGAGGTG AAGGAGCACC CTGGCACCAA GGAGCAGGAC 
TTGGAGAAGA AAAGCACAGA CACTCCTGAC AGGTCCTTCA AAAAGCGAAA GAGAGAAAAG 
GAGAAGGACG TCGATAGGAA GAAAAGGAAG TTGGGCAAAG AGGCAAAATC ATTGCAGTCT 
TCACCTAACA AAGACTCCTG TAAATTGAAA GCACGGAACG CTTCGCCTGA GACCCAGAAC 
AAAGATCTCA GGCAACCCCT GCCACTGCCG CCCATGTCTC CCACGCATCC TGCACCAAAG 
CCAACCAAGA AGGCCCACAA GAGGCCCAGG AGTGAGAACG GCCAGCCGCC TGCCACTGCC 
AGCAGCACTG CCCTGGATGC AAGCAACCAC AAGGATCCTT CTTCCACCAA GCAGAAGAAG 
ATGGTGGAAG ACCACTCTGA GCAGGTGAAG GGCAACAAAG GATCTGCAGG GGATGTCACA 
AATCCTTTAC CAGTGCCTTC TGTGCCAAAT GGTACCTCCA AACCAAGGAG ACCTCAGATC 
AAGTTTGAAA GACAGCATTT GTCAGAATAT TACCTGGAAG AGGCCAAGAA GATGAAGCAC 
GAGGCTGATG CCATGATGGA CAAGACTGGA AAGGCCTTTC AGTACCTCGA TGCTGCCCTT 
TCCTTTGTTG AATACGGGAT GGCCCTGGAA TCGAATGCGG TAGCACCAAA ACCTGCTTAC 
AGCATCTTTG CTGACACAAT AGATCTCCTC AAATACGTAA TGACACTAAA ACCCTTTACG 
GATGTCTCGG CATCTTCACA TGAACACGTC TTTGCTGTGT TATGCATGCG CTGCCAGTCC 
CTCCTGCACA TGGCAATGTT TCGTTACAAA AGAGACACTG CAATAAAGTA TTCCAGGATC 
CTGAATGACC ACTTCAAGAG TTCTTCCAGA GTAACACAGG CACCTTCTCC ATGTGTTGCA 
AGAAGCACAG GCATGCCTTC TCCTCATTCT CCAGTGCCCT CTCCTGCCAG ATCTATGAGT 
TCTCAGCAGG GATCAAATGG TAGCAATTGT GGTTGCAACA GCATTGGCTC TTCAGTCACC 
ATCCCAAGCA TCACCTCTTC ATACGTCAAC ATCACTTCCT ATGTCCTCTA TGCGTATAAT 
ATTTGGGAAC ATGCTGATGC ACTGTCCAAG AATAATAAGG AGTTTTTTGC TGAACTCAGC 
ACAGCGGTGT GCCCTCTGGC ACTGACCAGT AGCATGGCCG AAATGGTACA CTACACTAGA 
CAGGGTTTAC ATTGGCTGAG ACTGAACACA AGTACGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_004941100.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004941157.1 Gallus gallus AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA.

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CloneID	OGa08004
Clone ID Related Accession (Same CDS sequence)	XM_004941100.3 , XM_004941100.2
Accession Version	XM_004941100.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	AF4/FMR2 family member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004941100.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGC AGTCCAGCTT GTACAATGAG GACAGAAACT TGCTGCGTCT TCAGGAGAGA 
CAGAGGCGGA ATCAGGAGGC TCTGCAGGAG AAAGACAAGT TCCTTGAGAA CACCCCACTC 
TTTCCTGAGC CGTACAAGAC TAATAAAGAG GATGAGTTGT CAAGCCGTAT TCAGAATATG 
CTGGGCAATT ACGACGACTT GCAGGATCTT ATTAATGACA GATGCCATCA GAATCTCATA 
GGGATACCAG AGAGCGACGT TCCTCAGTTC CCACAAAGAA AGCCCGACCG CCCATCCTTC 
CCTGAAAAGA CCAGCCGTAC GTTATCGTCT TCATTCCACC ATGCAACCCG CTATCCACAC 
ATGGGACCCC CGGTCATTGC TCCTCCCCGT CCAGGCCACT ACGCTCCCCA CCAAAAGGCA 
CAACCAGGAA CAGGACCAGC TCCAGGCTGG CATGCCAAGA GTCACAGCTC GTCCAGCAGC 
TGGAGCCAAG GTCAGGAACA CAAGCGTGGC AGGCCGGACA GTCATGCCGG TTCTCACCAC 
AACAGAACTG ACAGGCATCC TGCTGGTGGA GGTCGTGCTC CTGAACCTCA GGCCACTCTT 
TTGGAGCTTT CCCCGTTGCT GCCGTCTTTG TCTTCTCCAG TGGCACCCCT GTCACCTCTA 
CATTCCATCC AGCACACTAA TTCCAAGTCA CAGAAGAGCA GCAAAGGTCA TGGGCCGGCC 
TACACTCAAT CACCTCAGGA CCGAGCGGTG GGTTCACGGG AGAATAAAAG TCAGGACAAC 
TCAGCCGTTA TCTTGGCTGG CACCACTCAG CCTTCCTCTC AACCCTTTCC TCCACCTTTG 
CCATCAAAAA CCAGTGCAAT GCAGCAGAAA CCAACTGCCT ACGTCAGACC AATGGATGGC 
CAAGAGCAGG CTCCGGTGGA GTCACCGGAT CTCAAGCCTC TACTGGAGGA ATACCACGGT 
GAACCGTATG AAAACATCTC AGATTTGAAG GCAAACACCA AGGCCAAATT ATCCAAATTG 
AACATCTCTT CCGAGCCCAC TGAGCAAACC TTGCCCAGTG ATAGCCAGTG CGTTGAAGAA 
ATTTTGAAGG AAATGACACA CTCATGGCCA CCTCTCTTGA CAGCTATTCA CACGCCAAGT 
GCTGCAGAGC CCTCCAAGTT TCCCTTTCCA ACAAAGGAAT CGCAGCATAT CCCATCTGTA 
GCACAGAATC ACAAGCAGTA TGACGCACCT TCCAAAACGT TGCCTAGTTC TCATCCAACA 
ATATCAGTGC TCCAGGAGGA CCTGCAGCTC AGTGATAGTG AAGAGAGTGG TGACGACCAA 
GTTGTTGAAC AGCCACCTTC TTCACTTGAT CCTCCAAGTG CCCTGCAGTC CCAGCCTGAG 
AGCGTGGCAT CAGCACATTC CAACAGCACA GAGTCAGGGA GCAGCAGTGA CTCAGACAGC 
TCTTCAGACT CTTCAGAGAC AGAGAGCAGC TCGAGTGACA GTGAAGCCAA CGACCCTCCA 
AGAGCGTCAG CTCCTGAGCC CGACCCACCA ACTTCCAATA AGTGGCAGCT TGACAACTGG 
CTCACCAAGG TGAACCCACC AGCAGTGCCA ACAGAAAGCC CCAGCGACAC TGCCCACACA 
GATGGCCACG AGGAGGACAA GGAACAACCC GTCAGCAACG CTTCCTCCTG CGAGCATGCT 
GTGCCAAGGG AACCTCACGA GAGAAGCTCC ATCCGAGCAG CCCAGGCTCC TCAGGATGTT 
CATCTTCCGA CCAAACTTAG CTGCCAGAAG TCTCCTGTGC GCGCTGAGGA GCCCTCACAG 
AGGCAGACTG TGGGGATCAA AAGGCCCAGC AAGCCTCCAG TGCACGAGGA ACCCAAGGGA 
GGCCTGAAGG TGGAGAGCGA GCCCGCTCCT TATGAGATTG GAGAGCAGTC TTCCAGAGAC 
AAGCCAAAAC CCAAAACAAA AAGCAGATCC AAATCCTGTG ACCAAAAAGA ACTGAAACCC 
CGGCTGCAGG AGGCTCCTAA AGAGAAGAAG CACAAGAGCT CCCACCACGC CGATGCCAAT 
GCCCTGCCGG ACCACAGACC CGTGAGTGAT GTTCTGGTAG GCAGTGCCCA GGAGCCTTCC 
ACTCTCAAAC ACCTCCCTGA GGGCCAGGGC ACAGCTCCCA CCAGGACTAA CGGCCACAGG 
TCTGCTGCTG AGGTCAAGGA GGGTTTTCAC AAGGGGAAGC TTCCTGTGCC CACCAAGGAG 
AAGAAGTTGC TGCCACCGGT GAGGGACGTG CCTGGCAATC CCAGCCTCCT GGTTAGAATT 
GATCTCCCAC TCCTGTCAAG AGTTCCCCAG CCTCCTGAGA AGGGGAACCA AAAGAAGAGA 
GCAGAGGTGA AGGAGCACCC TGGCACCAAG GAGCAGGACT TGGAGAAGAA AAGCACAGAC 
ACTCCTGACA GGTCCTTCAA AAAGCGAAAG AGAGAAAAGG AGAAGGACGT CGATAGGAAG 
AAAAGGAAGT TGGGCAAAGA GGCAAAATCA TTGCAGTCTT CACCTAACAA AGACTCCTGT 
AAATTGAAAG CACGGAACGC TTCGCCTGAG ACCCAGAACA AAGATCTCAG GCAACCCCTG 
CCACTGCCGC CCATGTCTCC CACGCATCCT GCACCAAAGC CAACCAAGAA GGCCCACAAG 
AGGCCCAGGA GTGAGAACGG CCAGCCGCCT GCCACTGCCA GCAGCACTGC CCTGGATGCA 
AGCAACCACA AGGATCCTTC TTCCACCAAG CAGAAGAAGA TGGTGGAAGA CCACTCTGAG 
CAGGTGAAGG GCAACAAAGG ATCTGCAGGG GATGTCACAA ATCCTTTACC AGTGCCTTCT 
GTGCCAAATG GTACCTCCAA ACCAAGGAGA CCTCAGATCA AGTTTGAAAG ACAGCATTTG 
TCAGAATATT ACCTGGAAGA GGCCAAGAAG ATGAAGCACG AGGCTGATGC CATGATGGAC 
AAGACTGGAA AGGCCTTTCA GTACCTCGAT GCTGCCCTTT CCTTTGTTGA ATACGGGATG 
GCCCTGGAAT CGAATGCGGT AGCACCAAAA CCTGCTTACA GCATCTTTGC TGACACAATA 
GATCTCCTCA AATACGTAAT GACACTAAAA CCCTTTACGG ATGTCTCGGC ATCTTCACAT 
GAACACGTCT TTGCTGTGTT ATGCATGCGC TGCCAGTCCC TCCTGCACAT GGCAATGTTT 
CGTTACAAAA GAGACACTGC AATAAAGTAT TCCAGGATCC TGAATGACCA CTTCAAGAGT 
TCTTCCAGAG TAACACAGGC ACCTTCTCCA TGTGTTGCAA GAAGCACAGG CATGCCTTCT 
CCTCATTCTC CAGTGCCCTC TCCTGCCAGA TCTATGAGTT CTCAGCAGGG ATCAAATGGT 
AGCAATTGTG GTTGCAACAG CATTGGCTCT TCAGTCACCA TCCCAAGCAT CACCTCTTCA 
TACGTCAACA TCACTTCCTA TGTCCTCTAT GCGTATAATA TTTGGGAACA TGCTGATGCA 
CTGTCCAAGA ATAATAAGGA GTTTTTTGCT GAACTCAGCA CAGCGGTGTG CCCTCTGGCA 
CTGACCAGTA GCATGGCCGA AATGGTACAC TACACTAGAC AGGGTTTACA TTGGCTGAGA 
CTGAACACAA GTACGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004941157.1
CDS	281..3901
Translation	MAAQSSLYNEDRNLLRLQERQRRNQEALQEKDKFLENTPLFPEPYKTNKEDELSSRIQNMLGNYDDLQDLINDRCHQNLIGIPESDVPQFPQRKPDRPSFPEKTSRTLSSSFHHATRYPHMGPPVIAPPRPGHYAPHQKAQPGTGPAPGWHAKSHSSSSSWSQGQEHKRGRPDSHAGSHHNRTDRHPAGGGRAPEPQATLLELSPLLPSLSSPVAPLSPLHSIQHTNSKSQKSSKGHGPAYTQSPQDRAVGSRENKSQDNSAVILAGTTQPSSQPFPPPLPSKTSAMQQKPTAYVRPMDGQEQAPVESPDLKPLLEEYHGEPYENISDLKANTKAKLSKLNISSEPTEQTLPSDSQCVEEILKEMTHSWPPLLTAIHTPSAAEPSKFPFPTKESQHIPSVAQNHKQYDAPSKTLPSSHPTISVLQEDLQLSDSEESGDDQVVEQPPSSLDPPSALQSQPESVASAHSNSTESGSSSDSDSSSDSSETESSSSDSEANDPPRASAPEPDPPTSNKWQLDNWLTKVNPPAVPTESPSDTAHTDGHEEDKEQPVSNASSCEHAVPREPHERSSIRAAQAPQDVHLPTKLSCQKSPVRAEEPSQRQTVGIKRPSKPPVHEEPKGGLKVESEPAPYEIGEQSSRDKPKPKTKSRSKSCDQKELKPRLQEAPKEKKHKSSHHADANALPDHRPVSDVLVGSAQEPSTLKHLPEGQGTAPTRTNGHRSAAEVKEGFHKGKLPVPTKEKKLLPPVRDVPGNPSLLVRIDLPLLSRVPQPPEKGNQKKRAEVKEHPGTKEQDLEKKSTDTPDRSFKKRKREKEKDVDRKKRKLGKEAKSLQSSPNKDSCKLKARNASPETQNKDLRQPLPLPPMSPTHPAPKPTKKAHKRPRSENGQPPATASSTALDASNHKDPSSTKQKKMVEDHSEQVKGNKGSAGDVTNPLPVPSVPNGTSKPRRPQIKFERQHLSEYYLEEAKKMKHEADAMMDKTGKAFQYLDAALSFVEYGMALESNAVAPKPAYSIFADTIDLLKYVMTLKPFTDVSASSHEHVFAVLCMRCQSLLHMAMFRYKRDTAIKYSRILNDHFKSSSRVTQAPSPCVARSTGMPSPHSPVPSPARSMSSQQGSNGSNCGCNSIGSSVTIPSITSSYVNITSYVLYAYNIWEHADALSKNNKEFFAELSTAVCPLALTSSMAEMVHYTRQGLHWLRLNTSTP

Target ORF information:

RefSeq Version	XM_004941100.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004941100.3

ORF Insert Sequence:

ATGGCCGCGC AGTCCAGCTT GTACAATGAG GACAGAAACT TGCTGCGTCT TCAGGAGAGA 
CAGAGGCGGA ATCAGGAGGC TCTGCAGGAG AAAGACAAGT TCCTTGAGAA CACCCCACTC 
TTTCCTGAGC CGTACAAGAC TAATAAAGAG GATGAGTTGT CAAGCCGTAT TCAGAATATG 
CTGGGCAATT ACGACGACTT GCAGGATCTT ATTAATGACA GATGCCATCA GAATCTCATA 
GGGATACCAG AGAGCGACGT TCCTCAGTTC CCACAAAGAA AGCCCGACCG CCCATCCTTC 
CCTGAAAAGA CCAGCCGTAC GTTATCGTCT TCATTCCACC ATGCAACCCG CTATCCACAC 
ATGGGACCCC CGGTCATTGC TCCTCCCCGT CCAGGCCACT ACGCTCCCCA CCAAAAGGCA 
CAACCAGGAA CAGGACCAGC TCCAGGCTGG CATGCCAAGA GTCACAGCTC GTCCAGCAGC 
TGGAGCCAAG GTCAGGAACA CAAGCGTGGC AGGCCGGACA GTCATGCCGG TTCTCACCAC 
AACAGAACTG ACAGGCATCC TGCTGGTGGA GGTCGTGCTC CTGAACCTCA GGCCACTCTT 
TTGGAGCTTT CCCCGTTGCT GCCGTCTTTG TCTTCTCCAG TGGCACCCCT GTCACCTCTA 
CATTCCATCC AGCACACTAA TTCCAAGTCA CAGAAGAGCA GCAAAGGTCA TGGGCCGGCC 
TACACTCAAT CACCTCAGGA CCGAGCGGTG GGTTCACGGG AGAATAAAAG TCAGGACAAC 
TCAGCCGTTA TCTTGGCTGG CACCACTCAG CCTTCCTCTC AACCCTTTCC TCCACCTTTG 
CCATCAAAAA CCAGTGCAAT GCAGCAGAAA CCAACTGCCT ACGTCAGACC AATGGATGGC 
CAAGAGCAGG CTCCGGTGGA GTCACCGGAT CTCAAGCCTC TACTGGAGGA ATACCACGGT 
GAACCGTATG AAAACATCTC AGATTTGAAG GCAAACACCA AGGCCAAATT ATCCAAATTG 
AACATCTCTT CCGAGCCCAC TGAGCAAACC TTGCCCAGTG ATAGCCAGTG CGTTGAAGAA 
ATTTTGAAGG AAATGACACA CTCATGGCCA CCTCTCTTGA CAGCTATTCA CACGCCAAGT 
GCTGCAGAGC CCTCCAAGTT TCCCTTTCCA ACAAAGGAAT CGCAGCATAT CCCATCTGTA 
GCACAGAATC ACAAGCAGTA TGACGCACCT TCCAAAACGT TGCCTAGTTC TCATCCAACA 
ATATCAGTGC TCCAGGAGGA CCTGCAGCTC AGTGATAGTG AAGAGAGTGG TGACGACCAA 
GTTGTTGAAC AGCCACCTTC TTCACTTGAT CCTCCAAGTG CCCTGCAGTC CCAGCCTGAG 
AGCGTGGCAT CAGCACATTC CAACAGCACA GAGTCAGGGA GCAGCAGTGA CTCAGACAGC 
TCTTCAGACT CTTCAGAGAC AGAGAGCAGC TCGAGTGACA GTGAAGCCAA CGACCCTCCA 
AGAGCGTCAG CTCCTGAGCC CGACCCACCA ACTTCCAATA AGTGGCAGCT TGACAACTGG 
CTCACCAAGG TGAACCCACC AGCAGTGCCA ACAGAAAGCC CCAGCGACAC TGCCCACACA 
GATGGCCACG AGGAGGACAA GGAACAACCC GTCAGCAACG CTTCCTCCTG CGAGCATGCT 
GTGCCAAGGG AACCTCACGA GAGAAGCTCC ATCCGAGCAG CCCAGGCTCC TCAGGATGTT 
CATCTTCCGA CCAAACTTAG CTGCCAGAAG TCTCCTGTGC GCGCTGAGGA GCCCTCACAG 
AGGCAGACTG TGGGGATCAA AAGGCCCAGC AAGCCTCCAG TGCACGAGGA ACCCAAGGGA 
GGCCTGAAGG TGGAGAGCGA GCCCGCTCCT TATGAGATTG GAGAGCAGTC TTCCAGAGAC 
AAGCCAAAAC CCAAAACAAA AAGCAGATCC AAATCCTGTG ACCAAAAAGA ACTGAAACCC 
CGGCTGCAGG AGGCTCCTAA AGAGAAGAAG CACAAGAGCT CCCACCACGC CGATGCCAAT 
GCCCTGCCGG ACCACAGACC CGTGAGTGAT GTTCTGGTAG GCAGTGCCCA GGAGCCTTCC 
ACTCTCAAAC ACCTCCCTGA GGGCCAGGGC ACAGCTCCCA CCAGGACTAA CGGCCACAGG 
TCTGCTGCTG AGGTCAAGGA GGGTTTTCAC AAGGGGAAGC TTCCTGTGCC CACCAAGGAG 
AAGAAGTTGC TGCCACCGGT GAGGGACGTG CCTGGCAATC CCAGCCTCCT GGTTAGAATT 
GATCTCCCAC TCCTGTCAAG AGTTCCCCAG CCTCCTGAGA AGGGGAACCA AAAGAAGAGA 
GCAGAGGTGA AGGAGCACCC TGGCACCAAG GAGCAGGACT TGGAGAAGAA AAGCACAGAC 
ACTCCTGACA GGTCCTTCAA AAAGCGAAAG AGAGAAAAGG AGAAGGACGT CGATAGGAAG 
AAAAGGAAGT TGGGCAAAGA GGCAAAATCA TTGCAGTCTT CACCTAACAA AGACTCCTGT 
AAATTGAAAG CACGGAACGC TTCGCCTGAG ACCCAGAACA AAGATCTCAG GCAACCCCTG 
CCACTGCCGC CCATGTCTCC CACGCATCCT GCACCAAAGC CAACCAAGAA GGCCCACAAG 
AGGCCCAGGA GTGAGAACGG CCAGCCGCCT GCCACTGCCA GCAGCACTGC CCTGGATGCA 
AGCAACCACA AGGATCCTTC TTCCACCAAG CAGAAGAAGA TGGTGGAAGA CCACTCTGAG 
CAGGTGAAGG GCAACAAAGG ATCTGCAGGG GATGTCACAA ATCCTTTACC AGTGCCTTCT 
GTGCCAAATG GTACCTCCAA ACCAAGGAGA CCTCAGATCA AGTTTGAAAG ACAGCATTTG 
TCAGAATATT ACCTGGAAGA GGCCAAGAAG ATGAAGCACG AGGCTGATGC CATGATGGAC 
AAGACTGGAA AGGCCTTTCA GTACCTCGAT GCTGCCCTTT CCTTTGTTGA ATACGGGATG 
GCCCTGGAAT CGAATGCGGT AGCACCAAAA CCTGCTTACA GCATCTTTGC TGACACAATA 
GATCTCCTCA AATACGTAAT GACACTAAAA CCCTTTACGG ATGTCTCGGC ATCTTCACAT 
GAACACGTCT TTGCTGTGTT ATGCATGCGC TGCCAGTCCC TCCTGCACAT GGCAATGTTT 
CGTTACAAAA GAGACACTGC AATAAAGTAT TCCAGGATCC TGAATGACCA CTTCAAGAGT 
TCTTCCAGAG TAACACAGGC ACCTTCTCCA TGTGTTGCAA GAAGCACAGG CATGCCTTCT 
CCTCATTCTC CAGTGCCCTC TCCTGCCAGA TCTATGAGTT CTCAGCAGGG ATCAAATGGT 
AGCAATTGTG GTTGCAACAG CATTGGCTCT TCAGTCACCA TCCCAAGCAT CACCTCTTCA 
TACGTCAACA TCACTTCCTA TGTCCTCTAT GCGTATAATA TTTGGGAACA TGCTGATGCA 
CTGTCCAAGA ATAATAAGGA GTTTTTTGCT GAACTCAGCA CAGCGGTGTG CCCTCTGGCA 
CTGACCAGTA GCATGGCCGA AATGGTACAC TACACTAGAC AGGGTTTACA TTGGCTGAGA 
CTGAACACAA GTACGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_004941100.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004941157.1 Gallus gallus AF4/FMR2 family, member 1 (AFF1), transcript variant X4, mRNA.

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CloneID	OGa08004
Clone ID Related Accession (Same CDS sequence)	XM_004941100.3 , XM_004941100.2
Accession Version	XM_004941100.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	AF4/FMR2 family member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004941100.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGC AGTCCAGCTT GTACAATGAG GACAGAAACT TGCTGCGTCT TCAGGAGAGA 
CAGAGGCGGA ATCAGGAGGC TCTGCAGGAG AAAGACAAGT TCCTTGAGAA CACCCCACTC 
TTTCCTGAGC CGTACAAGAC TAATAAAGAG GATGAGTTGT CAAGCCGTAT TCAGAATATG 
CTGGGCAATT ACGACGACTT GCAGGATCTT ATTAATGACA GATGCCATCA GAATCTCATA 
GGGATACCAG AGAGCGACGT TCCTCAGTTC CCACAAAGAA AGCCCGACCG CCCATCCTTC 
CCTGAAAAGA CCAGCCGTAC GTTATCGTCT TCATTCCACC ATGCAACCCG CTATCCACAC 
ATGGGACCCC CGGTCATTGC TCCTCCCCGT CCAGGCCACT ACGCTCCCCA CCAAAAGGCA 
CAACCAGGAA CAGGACCAGC TCCAGGCTGG CATGCCAAGA GTCACAGCTC GTCCAGCAGC 
TGGAGCCAAG GTCAGGAACA CAAGCGTGGC AGGCCGGACA GTCATGCCGG TTCTCACCAC 
AACAGAACTG ACAGGCATCC TGCTGGTGGA GGTCGTGCTC CTGAACCTCA GGCCACTCTT 
TTGGAGCTTT CCCCGTTGCT GCCGTCTTTG TCTTCTCCAG TGGCACCCCT GTCACCTCTA 
CATTCCATCC AGCACACTAA TTCCAAGTCA CAGAAGAGCA GCAAAGGTCA TGGGCCGGCC 
TACACTCAAT CACCTCAGGA CCGAGCGGTG GGTTCACGGG AGAATAAAAG TCAGGACAAC 
TCAGCCGTTA TCTTGGCTGG CACCACTCAG CCTTCCTCTC AACCCTTTCC TCCACCTTTG 
CCATCAAAAA CCAGTGCAAT GCAGCAGAAA CCAACTGCCT ACGTCAGACC AATGGATGGC 
CAAGAGCAGG CTCCGGTGGA GTCACCGGAT CTCAAGCCTC TACTGGAGGA ATACCACGGT 
GAACCGTATG AAAACATCTC AGATTTGAAG GCAAACACCA AGGCCAAATT ATCCAAATTG 
AACATCTCTT CCGAGCCCAC TGAGCAAACC TTGCCCAGTG ATAGCCAGTG CGTTGAAGAA 
ATTTTGAAGG AAATGACACA CTCATGGCCA CCTCTCTTGA CAGCTATTCA CACGCCAAGT 
GCTGCAGAGC CCTCCAAGTT TCCCTTTCCA ACAAAGGAAT CGCAGCATAT CCCATCTGTA 
GCACAGAATC ACAAGCAGTA TGACGCACCT TCCAAAACGT TGCCTAGTTC TCATCCAACA 
ATATCAGTGC TCCAGGAGGA CCTGCAGCTC AGTGATAGTG AAGAGAGTGG TGACGACCAA 
GTTGTTGAAC AGCCACCTTC TTCACTTGAT CCTCCAAGTG CCCTGCAGTC CCAGCCTGAG 
AGCGTGGCAT CAGCACATTC CAACAGCACA GAGTCAGGGA GCAGCAGTGA CTCAGACAGC 
TCTTCAGACT CTTCAGAGAC AGAGAGCAGC TCGAGTGACA GTGAAGCCAA CGACCCTCCA 
AGAGCGTCAG CTCCTGAGCC CGACCCACCA ACTTCCAATA AGTGGCAGCT TGACAACTGG 
CTCACCAAGG TGAACCCACC AGCAGTGCCA ACAGAAAGCC CCAGCGACAC TGCCCACACA 
GATGGCCACG AGGAGGACAA GGAACAACCC GTCAGCAACG CTTCCTCCTG CGAGCATGCT 
GTGCCAAGGG AACCTCACGA GAGAAGCTCC ATCCGAGCAG CCCAGGCTCC TCAGGATGTT 
CATCTTCCGA CCAAACTTAG CTGCCAGAAG TCTCCTGTGC GCGCTGAGGA GCCCTCACAG 
AGGCAGACTG TGGGGATCAA AAGGCCCAGC AAGCCTCCAG TGCACGAGGA ACCCAAGGGA 
GGCCTGAAGG TGGAGAGCGA GCCCGCTCCT TATGAGATTG GAGAGCAGTC TTCCAGAGAC 
AAGCCAAAAC CCAAAACAAA AAGCAGATCC AAATCCTGTG ACCAAAAAGA ACTGAAACCC 
CGGCTGCAGG AGGCTCCTAA AGAGAAGAAG CACAAGAGCT CCCACCACGC CGATGCCAAT 
GCCCTGCCGG ACCACAGACC CGTGAGTGAT GTTCTGGTAG GCAGTGCCCA GGAGCCTTCC 
ACTCTCAAAC ACCTCCCTGA GGGCCAGGGC ACAGCTCCCA CCAGGACTAA CGGCCACAGG 
TCTGCTGCTG AGGTCAAGGA GGGTTTTCAC AAGGGGAAGC TTCCTGTGCC CACCAAGGAG 
AAGAAGTTGC TGCCACCGGT GAGGGACGTG CCTGGCAATC CCAGCCTCCT GGTTAGAATT 
GATCTCCCAC TCCTGTCAAG AGTTCCCCAG CCTCCTGAGA AGGGGAACCA AAAGAAGAGA 
GCAGAGGTGA AGGAGCACCC TGGCACCAAG GAGCAGGACT TGGAGAAGAA AAGCACAGAC 
ACTCCTGACA GGTCCTTCAA AAAGCGAAAG AGAGAAAAGG AGAAGGACGT CGATAGGAAG 
AAAAGGAAGT TGGGCAAAGA GGCAAAATCA TTGCAGTCTT CACCTAACAA AGACTCCTGT 
AAATTGAAAG CACGGAACGC TTCGCCTGAG ACCCAGAACA AAGATCTCAG GCAACCCCTG 
CCACTGCCGC CCATGTCTCC CACGCATCCT GCACCAAAGC CAACCAAGAA GGCCCACAAG 
AGGCCCAGGA GTGAGAACGG CCAGCCGCCT GCCACTGCCA GCAGCACTGC CCTGGATGCA 
AGCAACCACA AGGATCCTTC TTCCACCAAG CAGAAGAAGA TGGTGGAAGA CCACTCTGAG 
CAGGTGAAGG GCAACAAAGG ATCTGCAGGG GATGTCACAA ATCCTTTACC AGTGCCTTCT 
GTGCCAAATG GTACCTCCAA ACCAAGGAGA CCTCAGATCA AGTTTGAAAG ACAGCATTTG 
TCAGAATATT ACCTGGAAGA GGCCAAGAAG ATGAAGCACG AGGCTGATGC CATGATGGAC 
AAGACTGGAA AGGCCTTTCA GTACCTCGAT GCTGCCCTTT CCTTTGTTGA ATACGGGATG 
GCCCTGGAAT CGAATGCGGT AGCACCAAAA CCTGCTTACA GCATCTTTGC TGACACAATA 
GATCTCCTCA AATACGTAAT GACACTAAAA CCCTTTACGG ATGTCTCGGC ATCTTCACAT 
GAACACGTCT TTGCTGTGTT ATGCATGCGC TGCCAGTCCC TCCTGCACAT GGCAATGTTT 
CGTTACAAAA GAGACACTGC AATAAAGTAT TCCAGGATCC TGAATGACCA CTTCAAGAGT 
TCTTCCAGAG TAACACAGGC ACCTTCTCCA TGTGTTGCAA GAAGCACAGG CATGCCTTCT 
CCTCATTCTC CAGTGCCCTC TCCTGCCAGA TCTATGAGTT CTCAGCAGGG ATCAAATGGT 
AGCAATTGTG GTTGCAACAG CATTGGCTCT TCAGTCACCA TCCCAAGCAT CACCTCTTCA 
TACGTCAACA TCACTTCCTA TGTCCTCTAT GCGTATAATA TTTGGGAACA TGCTGATGCA 
CTGTCCAAGA ATAATAAGGA GTTTTTTGCT GAACTCAGCA CAGCGGTGTG CCCTCTGGCA 
CTGACCAGTA GCATGGCCGA AATGGTACAC TACACTAGAC AGGGTTTACA TTGGCTGAGA 
CTGAACACAA GTACGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004941157.1
CDS	281..3901
Translation	MAAQSSLYNEDRNLLRLQERQRRNQEALQEKDKFLENTPLFPEPYKTNKEDELSSRIQNMLGNYDDLQDLINDRCHQNLIGIPESDVPQFPQRKPDRPSFPEKTSRTLSSSFHHATRYPHMGPPVIAPPRPGHYAPHQKAQPGTGPAPGWHAKSHSSSSSWSQGQEHKRGRPDSHAGSHHNRTDRHPAGGGRAPEPQATLLELSPLLPSLSSPVAPLSPLHSIQHTNSKSQKSSKGHGPAYTQSPQDRAVGSRENKSQDNSAVILAGTTQPSSQPFPPPLPSKTSAMQQKPTAYVRPMDGQEQAPVESPDLKPLLEEYHGEPYENISDLKANTKAKLSKLNISSEPTEQTLPSDSQCVEEILKEMTHSWPPLLTAIHTPSAAEPSKFPFPTKESQHIPSVAQNHKQYDAPSKTLPSSHPTISVLQEDLQLSDSEESGDDQVVEQPPSSLDPPSALQSQPESVASAHSNSTESGSSSDSDSSSDSSETESSSSDSEANDPPRASAPEPDPPTSNKWQLDNWLTKVNPPAVPTESPSDTAHTDGHEEDKEQPVSNASSCEHAVPREPHERSSIRAAQAPQDVHLPTKLSCQKSPVRAEEPSQRQTVGIKRPSKPPVHEEPKGGLKVESEPAPYEIGEQSSRDKPKPKTKSRSKSCDQKELKPRLQEAPKEKKHKSSHHADANALPDHRPVSDVLVGSAQEPSTLKHLPEGQGTAPTRTNGHRSAAEVKEGFHKGKLPVPTKEKKLLPPVRDVPGNPSLLVRIDLPLLSRVPQPPEKGNQKKRAEVKEHPGTKEQDLEKKSTDTPDRSFKKRKREKEKDVDRKKRKLGKEAKSLQSSPNKDSCKLKARNASPETQNKDLRQPLPLPPMSPTHPAPKPTKKAHKRPRSENGQPPATASSTALDASNHKDPSSTKQKKMVEDHSEQVKGNKGSAGDVTNPLPVPSVPNGTSKPRRPQIKFERQHLSEYYLEEAKKMKHEADAMMDKTGKAFQYLDAALSFVEYGMALESNAVAPKPAYSIFADTIDLLKYVMTLKPFTDVSASSHEHVFAVLCMRCQSLLHMAMFRYKRDTAIKYSRILNDHFKSSSRVTQAPSPCVARSTGMPSPHSPVPSPARSMSSQQGSNGSNCGCNSIGSSVTIPSITSSYVNITSYVLYAYNIWEHADALSKNNKEFFAELSTAVCPLALTSSMAEMVHYTRQGLHWLRLNTSTP

Target ORF information:

RefSeq Version	XM_004941100.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus AF4/FMR2 family, member 1 (AFF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004941100.2

ORF Insert Sequence:

ATGGCCGCGC AGTCCAGCTT GTACAATGAG GACAGAAACT TGCTGCGTCT TCAGGAGAGA 
CAGAGGCGGA ATCAGGAGGC TCTGCAGGAG AAAGACAAGT TCCTTGAGAA CACCCCACTC 
TTTCCTGAGC CGTACAAGAC TAATAAAGAG GATGAGTTGT CAAGCCGTAT TCAGAATATG 
CTGGGCAATT ACGACGACTT GCAGGATCTT ATTAATGACA GATGCCATCA GAATCTCATA 
GGGATACCAG AGAGCGACGT TCCTCAGTTC CCACAAAGAA AGCCCGACCG CCCATCCTTC 
CCTGAAAAGA CCAGCCGTAC GTTATCGTCT TCATTCCACC ATGCAACCCG CTATCCACAC 
ATGGGACCCC CGGTCATTGC TCCTCCCCGT CCAGGCCACT ACGCTCCCCA CCAAAAGGCA 
CAACCAGGAA CAGGACCAGC TCCAGGCTGG CATGCCAAGA GTCACAGCTC GTCCAGCAGC 
TGGAGCCAAG GTCAGGAACA CAAGCGTGGC AGGCCGGACA GTCATGCCGG TTCTCACCAC 
AACAGAACTG ACAGGCATCC TGCTGGTGGA GGTCGTGCTC CTGAACCTCA GGCCACTCTT 
TTGGAGCTTT CCCCGTTGCT GCCGTCTTTG TCTTCTCCAG TGGCACCCCT GTCACCTCTA 
CATTCCATCC AGCACACTAA TTCCAAGTCA CAGAAGAGCA GCAAAGGTCA TGGGCCGGCC 
TACACTCAAT CACCTCAGGA CCGAGCGGTG GGTTCACGGG AGAATAAAAG TCAGGACAAC 
TCAGCCGTTA TCTTGGCTGG CACCACTCAG CCTTCCTCTC AACCCTTTCC TCCACCTTTG 
CCATCAAAAA CCAGTGCAAT GCAGCAGAAA CCAACTGCCT ACGTCAGACC AATGGATGGC 
CAAGAGCAGG CTCCGGTGGA GTCACCGGAT CTCAAGCCTC TACTGGAGGA ATACCACGGT 
GAACCGTATG AAAACATCTC AGATTTGAAG GCAAACACCA AGGCCAAATT ATCCAAATTG 
AACATCTCTT CCGAGCCCAC TGAGCAAACC TTGCCCAGTG ATAGCCAGTG CGTTGAAGAA 
ATTTTGAAGG AAATGACACA CTCATGGCCA CCTCTCTTGA CAGCTATTCA CACGCCAAGT 
GCTGCAGAGC CCTCCAAGTT TCCCTTTCCA ACAAAGGAAT CGCAGCATAT CCCATCTGTA 
GCACAGAATC ACAAGCAGTA TGACGCACCT TCCAAAACGT TGCCTAGTTC TCATCCAACA 
ATATCAGTGC TCCAGGAGGA CCTGCAGCTC AGTGATAGTG AAGAGAGTGG TGACGACCAA 
GTTGTTGAAC AGCCACCTTC TTCACTTGAT CCTCCAAGTG CCCTGCAGTC CCAGCCTGAG 
AGCGTGGCAT CAGCACATTC CAACAGCACA GAGTCAGGGA GCAGCAGTGA CTCAGACAGC 
TCTTCAGACT CTTCAGAGAC AGAGAGCAGC TCGAGTGACA GTGAAGCCAA CGACCCTCCA 
AGAGCGTCAG CTCCTGAGCC CGACCCACCA ACTTCCAATA AGTGGCAGCT TGACAACTGG 
CTCACCAAGG TGAACCCACC AGCAGTGCCA ACAGAAAGCC CCAGCGACAC TGCCCACACA 
GATGGCCACG AGGAGGACAA GGAACAACCC GTCAGCAACG CTTCCTCCTG CGAGCATGCT 
GTGCCAAGGG AACCTCACGA GAGAAGCTCC ATCCGAGCAG CCCAGGCTCC TCAGGATGTT 
CATCTTCCGA CCAAACTTAG CTGCCAGAAG TCTCCTGTGC GCGCTGAGGA GCCCTCACAG 
AGGCAGACTG TGGGGATCAA AAGGCCCAGC AAGCCTCCAG TGCACGAGGA ACCCAAGGGA 
GGCCTGAAGG TGGAGAGCGA GCCCGCTCCT TATGAGATTG GAGAGCAGTC TTCCAGAGAC 
AAGCCAAAAC CCAAAACAAA AAGCAGATCC AAATCCTGTG ACCAAAAAGA ACTGAAACCC 
CGGCTGCAGG AGGCTCCTAA AGAGAAGAAG CACAAGAGCT CCCACCACGC CGATGCCAAT 
GCCCTGCCGG ACCACAGACC CGTGAGTGAT GTTCTGGTAG GCAGTGCCCA GGAGCCTTCC 
ACTCTCAAAC ACCTCCCTGA GGGCCAGGGC ACAGCTCCCA CCAGGACTAA CGGCCACAGG 
TCTGCTGCTG AGGTCAAGGA GGGTTTTCAC AAGGGGAAGC TTCCTGTGCC CACCAAGGAG 
AAGAAGTTGC TGCCACCGGT GAGGGACGTG CCTGGCAATC CCAGCCTCCT GGTTAGAATT 
GATCTCCCAC TCCTGTCAAG AGTTCCCCAG CCTCCTGAGA AGGGGAACCA AAAGAAGAGA 
GCAGAGGTGA AGGAGCACCC TGGCACCAAG GAGCAGGACT TGGAGAAGAA AAGCACAGAC 
ACTCCTGACA GGTCCTTCAA AAAGCGAAAG AGAGAAAAGG AGAAGGACGT CGATAGGAAG 
AAAAGGAAGT TGGGCAAAGA GGCAAAATCA TTGCAGTCTT CACCTAACAA AGACTCCTGT 
AAATTGAAAG CACGGAACGC TTCGCCTGAG ACCCAGAACA AAGATCTCAG GCAACCCCTG 
CCACTGCCGC CCATGTCTCC CACGCATCCT GCACCAAAGC CAACCAAGAA GGCCCACAAG 
AGGCCCAGGA GTGAGAACGG CCAGCCGCCT GCCACTGCCA GCAGCACTGC CCTGGATGCA 
AGCAACCACA AGGATCCTTC TTCCACCAAG CAGAAGAAGA TGGTGGAAGA CCACTCTGAG 
CAGGTGAAGG GCAACAAAGG ATCTGCAGGG GATGTCACAA ATCCTTTACC AGTGCCTTCT 
GTGCCAAATG GTACCTCCAA ACCAAGGAGA CCTCAGATCA AGTTTGAAAG ACAGCATTTG 
TCAGAATATT ACCTGGAAGA GGCCAAGAAG ATGAAGCACG AGGCTGATGC CATGATGGAC 
AAGACTGGAA AGGCCTTTCA GTACCTCGAT GCTGCCCTTT CCTTTGTTGA ATACGGGATG 
GCCCTGGAAT CGAATGCGGT AGCACCAAAA CCTGCTTACA GCATCTTTGC TGACACAATA 
GATCTCCTCA AATACGTAAT GACACTAAAA CCCTTTACGG ATGTCTCGGC ATCTTCACAT 
GAACACGTCT TTGCTGTGTT ATGCATGCGC TGCCAGTCCC TCCTGCACAT GGCAATGTTT 
CGTTACAAAA GAGACACTGC AATAAAGTAT TCCAGGATCC TGAATGACCA CTTCAAGAGT 
TCTTCCAGAG TAACACAGGC ACCTTCTCCA TGTGTTGCAA GAAGCACAGG CATGCCTTCT 
CCTCATTCTC CAGTGCCCTC TCCTGCCAGA TCTATGAGTT CTCAGCAGGG ATCAAATGGT 
AGCAATTGTG GTTGCAACAG CATTGGCTCT TCAGTCACCA TCCCAAGCAT CACCTCTTCA 
TACGTCAACA TCACTTCCTA TGTCCTCTAT GCGTATAATA TTTGGGAACA TGCTGATGCA 
CTGTCCAAGA ATAATAAGGA GTTTTTTGCT GAACTCAGCA CAGCGGTGTG CCCTCTGGCA 
CTGACCAGTA GCATGGCCGA AATGGTACAC TACACTAGAC AGGGTTTACA TTGGCTGAGA 
CTGAACACAA GTACGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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AFF1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

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