AFF1 cDNA ORF clone, Bos taurus(cattle)

The following AFF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa235459 XM_024993467.1
Latest version!
Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa239036 NM_001191525.3
Latest version!
Bos taurus AF4/FMR2 family member 1 (AFF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235455 XM_005208211.4
Latest version!
Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235455 XM_010806299.3
Latest version!
Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235456 XM_024993466.1
Latest version!
Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235456 XM_010806300.3
Latest version!
Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235457 XM_010806302.3
Latest version!
Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235458 XM_005208213.4
Latest version!
Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa01736 NM_001191525.1
Latest version!
Bos taurus AF4/FMR2 family, member 1 (AFF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa216353 NM_001191525.2
Latest version!
Bos taurus AF4/FMR2 family member 1 (AFF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa20595 XM_005208211.3
Latest version!
Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa20595 XM_010806299.2
Latest version!
Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa20597 XM_005208213.3
Latest version!
Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa57021 XM_010806300.2
Latest version!
Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa57022 XM_010806302.2
Latest version!
Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa235459
    Clone ID Related Accession (Same CDS sequence) XM_024993467.1
    Accession Version XM_024993467.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGACCCACT CATGGCCTCC TCCTTTGACA GCAATACACA CGCCTAGTAC AGCTGAGCCC 
    TCCAAATTTC CTTTTCCCAC AAAGGATTCT CAGCACATCA GTTCTGCGGT CCCAAACCAA
    AAACAATATG ATACATCTTC AAAAACTCAC AGTAATTCTC AGCAGGGAAC ATCCATGCTC
    AAAGATGACC TTCAGCTCAG TGACAGTGAG GACAGTGACA GCGATCAAAC CTCAGAGAAG
    CCTCCTTTCG CACCTGCACC TCCAAGCGCT CCACCGGCGC TGCCAGCAGC CGCGGCCTCA
    GCACATTCCA GCAGCGCCGA ATCGGAGAGC ACCAGCGACT CCGACAGCTC CTCGGACTCG
    GAGAGCGAGA GCAGCTCCAG TGACAGCGAG GAGAACGAAC CCCGAGAAGC CCCGGCTCCC
    GAGCCCGAGC CTCCGACAAC CAACAAATGG CAGCTGGACA ACTGGCTGAC CAAAGTCAGC
    CAGCCCGCGG TGCCCACCGA GGCCCCGGGT AGCACGGAGC CCCCGGCCCG ACCCCCGGAC
    ACTAAGGGCA AGGGCGGCGA CAGCGCCACC GCGGGTCACG AGCGCCCAGA GTCCAAAGAG
    CCTCCCCCCA AAGGCTCCAG CAAAGCCCCC CGGGTCCCAT CCGAAGGCCC CCACGCGGGC
    AAGAGGAGCT GTCAGAAGTC CCCTACCCAG CAGGAGCCCC CCCAGCGGCA AACCGTGGGA
    AGCAAACAGC CGAAAAAGCC CATCAAGGCT TCCGCCCCCG GGCACGCGGA TGCGCCTGCA
    GATGCGCGTG CCTGCCTGCA GGTGGAGAGC GAGCCCGGAC TTCCTCCCCC GGCCTCCAGG
    GACCAGCCCT CCAAAGACAA GCCCAAGGTG AAGACGAAAG GGCGGCCCCG CGCCGCAGAC
    AGCCGGGAGC CCAAGCCGGC GGTGCCCACC CCCAGCGACC GGAAGAAGCA CCGCAGCGGC
    CCCCCGAAGG CGCCCCCGAA GGACGCGGCG GCAGAGGACC GGAGCCCCGA ACACTTTGCG
    CTCGTTCCCC TGACCCAGAG CCAGGGCCCT GTCCGCGGTG GCAGCGGCGG CGCCGGCGCC
    AGGACTAGTG GCTGCAGGCT GGCTGTGGTG GTCCAGGAGG ACCGCCGCAA AGACAAACCC
    CCGGTGCCTT CGAGAGACAC CAAGCTGCTC TCCCCGCTCA GGGACGCTCC GCCGCCACAA
    AGCTTGGTGG TGAAGATCAC ACTCGACCTC CTGTCTCGGA TACCCCAGCC CCCAGGGAAG
    GGCAGCCGCC CCAAGAAACC AGAAGACAAA CAGCCACCAG CAGGGAAGAA GCCAGATCCT
    GAGAAGAGAA GCTCAGAAAA CGCCAGCAAG TTAGCCAAAA AGAGAAAGGG TGAAGCAGAG
    AAAGACCACG ATAGCAAGAA AGTCAGGCTG GAGAAGGAAG TCAAGTCACA GCCGTTGTCT
    TCCTCTTCCC ACAAGGAATC TTCCAAATCA AAGATGTCCA GGCCCTCCTC TGAGCCCTCA
    AAGAAGGAAA TGCTTCCCCC TTCGCTCGTG TCATCCTCAT CCTCCCAGAA GCCAGCCAAG
    CCTGCACAGA AGAGGCCACG GCAGGAAGAC CCCGGCTGCC AGGACCCCCA AAAAAGTGCC
    GGTAGTGCCA AGGGCAGCCA CAGAGACCCT TCTGCCTCCA AACACAGAAA AGCAGAGGGA
    AAGGTCTCTG GAAGCTCCAC AGAGCACAAA GGATCTTCTG GAGATACTGC AAACCGTTTT
    CCAGTGCCTT CTTTGCCAAA TGGTAACTCC AAACCAGGGA AGCCTCACGT GAAGTTTGAC
    AAACAACAAG CCGATTTTCA CCTGAAGGAA GCCAAAAGGT TGAAGGACAA AGCAGAATTA
    GTGACGGACA AGGTAGGGAA GGCCTTTAAA TACCTGGAGG CTGCCTTGTC CTTCATTGAG
    TGCGGCGTCG CCCTGGAGTC GGAGAGCCCC GCGTCCAAGT CGGCGTACTC CATCTACTCG
    GAGACCGTGG ACCTCATTAA GTTCATACTG TCATTAAAAT CCTTCTCAGA TGCCACAACG
    CCAACACAGG AGAAGATATT TGCTGTTCTA TGCATGCGTT GCCAGTCCAT TTTGAACATG
    GCAATGTTTC GTTGTAAAAA AGATATGGCG ATAAAGTATT CCCGCACGCT TAACGAACAC
    TTCTTCAAGA CTTCTTCCAA GGTTGCCCAG GCACCTTCTC CATGCATCGC AAGAAGCACA
    GGCACACCGT CCCCCCTCTC TCCGATCCCT TCTCCGGCCA GCTCCATAGG GTCCCAGTCA
    AGCGCTGGCA GCGTGGGGAG CGGGGCGCTG CCCGCCACCA TCAGCACCCC CGTCACCATC
    CAGAACATGA CATCCTCCTA CGTCAGCATC ACTTCCCACG TCCTTACCGC CTTTGACCTG
    TGGGAACAGG CTGAGGTCCT TACCAGGAAG AACAAAGAAT TCTTTGCTCA GCTCAGCACA
    AGTGTGTGCG CGCTGGCCCT CAACAGCAGT TTGATGGATC TGGTGCATTA TACAAGACAG
    GGTTTCCAGC GGCTAAAGCA AGTAACCAAG ACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849235.1
    CDS123..2681
    Translation

    Target ORF information:

    RefSeq Version XM_024993467.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993467.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGACCCACT CATGGCCTCC TCCTTTGACA GCAATACACA CGCCTAGTAC AGCTGAGCCC 
    TCCAAATTTC CTTTTCCCAC AAAGGATTCT CAGCACATCA GTTCTGCGGT CCCAAACCAA
    AAACAATATG ATACATCTTC AAAAACTCAC AGTAATTCTC AGCAGGGAAC ATCCATGCTC
    AAAGATGACC TTCAGCTCAG TGACAGTGAG GACAGTGACA GCGATCAAAC CTCAGAGAAG
    CCTCCTTTCG CACCTGCACC TCCAAGCGCT CCACCGGCGC TGCCAGCAGC CGCGGCCTCA
    GCACATTCCA GCAGCGCCGA ATCGGAGAGC ACCAGCGACT CCGACAGCTC CTCGGACTCG
    GAGAGCGAGA GCAGCTCCAG TGACAGCGAG GAGAACGAAC CCCGAGAAGC CCCGGCTCCC
    GAGCCCGAGC CTCCGACAAC CAACAAATGG CAGCTGGACA ACTGGCTGAC CAAAGTCAGC
    CAGCCCGCGG TGCCCACCGA GGCCCCGGGT AGCACGGAGC CCCCGGCCCG ACCCCCGGAC
    ACTAAGGGCA AGGGCGGCGA CAGCGCCACC GCGGGTCACG AGCGCCCAGA GTCCAAAGAG
    CCTCCCCCCA AAGGCTCCAG CAAAGCCCCC CGGGTCCCAT CCGAAGGCCC CCACGCGGGC
    AAGAGGAGCT GTCAGAAGTC CCCTACCCAG CAGGAGCCCC CCCAGCGGCA AACCGTGGGA
    AGCAAACAGC CGAAAAAGCC CATCAAGGCT TCCGCCCCCG GGCACGCGGA TGCGCCTGCA
    GATGCGCGTG CCTGCCTGCA GGTGGAGAGC GAGCCCGGAC TTCCTCCCCC GGCCTCCAGG
    GACCAGCCCT CCAAAGACAA GCCCAAGGTG AAGACGAAAG GGCGGCCCCG CGCCGCAGAC
    AGCCGGGAGC CCAAGCCGGC GGTGCCCACC CCCAGCGACC GGAAGAAGCA CCGCAGCGGC
    CCCCCGAAGG CGCCCCCGAA GGACGCGGCG GCAGAGGACC GGAGCCCCGA ACACTTTGCG
    CTCGTTCCCC TGACCCAGAG CCAGGGCCCT GTCCGCGGTG GCAGCGGCGG CGCCGGCGCC
    AGGACTAGTG GCTGCAGGCT GGCTGTGGTG GTCCAGGAGG ACCGCCGCAA AGACAAACCC
    CCGGTGCCTT CGAGAGACAC CAAGCTGCTC TCCCCGCTCA GGGACGCTCC GCCGCCACAA
    AGCTTGGTGG TGAAGATCAC ACTCGACCTC CTGTCTCGGA TACCCCAGCC CCCAGGGAAG
    GGCAGCCGCC CCAAGAAACC AGAAGACAAA CAGCCACCAG CAGGGAAGAA GCCAGATCCT
    GAGAAGAGAA GCTCAGAAAA CGCCAGCAAG TTAGCCAAAA AGAGAAAGGG TGAAGCAGAG
    AAAGACCACG ATAGCAAGAA AGTCAGGCTG GAGAAGGAAG TCAAGTCACA GCCGTTGTCT
    TCCTCTTCCC ACAAGGAATC TTCCAAATCA AAGATGTCCA GGCCCTCCTC TGAGCCCTCA
    AAGAAGGAAA TGCTTCCCCC TTCGCTCGTG TCATCCTCAT CCTCCCAGAA GCCAGCCAAG
    CCTGCACAGA AGAGGCCACG GCAGGAAGAC CCCGGCTGCC AGGACCCCCA AAAAAGTGCC
    GGTAGTGCCA AGGGCAGCCA CAGAGACCCT TCTGCCTCCA AACACAGAAA AGCAGAGGGA
    AAGGTCTCTG GAAGCTCCAC AGAGCACAAA GGATCTTCTG GAGATACTGC AAACCGTTTT
    CCAGTGCCTT CTTTGCCAAA TGGTAACTCC AAACCAGGGA AGCCTCACGT GAAGTTTGAC
    AAACAACAAG CCGATTTTCA CCTGAAGGAA GCCAAAAGGT TGAAGGACAA AGCAGAATTA
    GTGACGGACA AGGTAGGGAA GGCCTTTAAA TACCTGGAGG CTGCCTTGTC CTTCATTGAG
    TGCGGCGTCG CCCTGGAGTC GGAGAGCCCC GCGTCCAAGT CGGCGTACTC CATCTACTCG
    GAGACCGTGG ACCTCATTAA GTTCATACTG TCATTAAAAT CCTTCTCAGA TGCCACAACG
    CCAACACAGG AGAAGATATT TGCTGTTCTA TGCATGCGTT GCCAGTCCAT TTTGAACATG
    GCAATGTTTC GTTGTAAAAA AGATATGGCG ATAAAGTATT CCCGCACGCT TAACGAACAC
    TTCTTCAAGA CTTCTTCCAA GGTTGCCCAG GCACCTTCTC CATGCATCGC AAGAAGCACA
    GGCACACCGT CCCCCCTCTC TCCGATCCCT TCTCCGGCCA GCTCCATAGG GTCCCAGTCA
    AGCGCTGGCA GCGTGGGGAG CGGGGCGCTG CCCGCCACCA TCAGCACCCC CGTCACCATC
    CAGAACATGA CATCCTCCTA CGTCAGCATC ACTTCCCACG TCCTTACCGC CTTTGACCTG
    TGGGAACAGG CTGAGGTCCT TACCAGGAAG AACAAAGAAT TCTTTGCTCA GCTCAGCACA
    AGTGTGTGCG CGCTGGCCCT CAACAGCAGT TTGATGGATC TGGTGCATTA TACAAGACAG
    GGTTTCCAGC GGCTAAAGCA AGTAACCAAG ACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa239036
    Clone ID Related Accession (Same CDS sequence) NM_001191525.3
    Accession Version NM_001191525.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000006.1. On May 30, 2018 this sequence version replaced NM_001191525.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCCC AGTCAAGCTT GTACAATGAA GACAGAAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC TCACCAAGAT AAAGAGGCAT TTTCTGAAAA GATCCCCCTT
    TTTGGAGAGC CATACAAGAC AGAGAAAGGT GATGAGCTAT CCAGTCGGAT ACAGAACATG
    CTGGGAAACT ATGAAGAGAT GAAGGAGTTC CTTAGTAGCA AGTCCCACCC CCAGCGCTTG
    GATGCTTCTG AAAATAGGCT GGGGAAGCCG AGATACCCTT TATTTCCTGA GAAGGGGAGC
    AGCCTTCCAC CCCACTCCTT CCACACCAGT GTCCACCACC AGCCCGTTAA CACTCCTGCC
    TGTGGGCCAC ATCCTGCTGG TAACATCGGC CACCACCCAA AGATGGCGCA GCCAAGAATG
    GAACCAATGC CAAGTCTCCA CGCCAAAAGC TATGGCCCAC CGGACAGCCA GCACCTGACC
    CAAGAGCGCC TCGGTCAGGA GGGGTACGGC TCTAGTCATC ACAAAAAGGG TGACCGCAGG
    GCTGATGGAG ACCACTGTGC TTTGACGACA GACTCTGCTC CGGAGAGGGA ACTTTCACCT
    CTGTTCTCTT TGCCTTCCCC AGTCCCCCCT TTGTCACCTG TACATTCCAA CCAGCAGACT
    GCTCCCAGGA CGCAAGGAAG CGGCAAAGTT CCCAGCAGTA GCAGTAGCAA TAAAGGCTAC
    TGCCTGGCCA AGTCTCCCAA GGACCTAGCG GTAAAGGTGC ACGATAAAGA GACCCCTCAA
    GACAGCACAG GGGCAGTGGC CAGCCTTGGG GTAGCCCCTC CTCAGCCACC TTCTCAGACC
    TTCCCCCCAC CCTCCCTCCC CTCAAAAAGC CTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGACGGTCA AGACCAGGCT CCTAGCGAGT CTCCTGAACT GAAACCCCTG
    CCGGAGGAGT ACCGGCAGCA GACCTTTGAG AAATCAGACT TAAAAGTGCC TGCCAAAGCC
    AAGCTCACGA AGCTGAAAAT GCCTTCTCAG TCAGTCGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCACTCAT GGCCTCCTCC TTTGACAGCA
    ATACACACGC CTAGTACAGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG
    CACATCAGTT CTGCGGTCCC AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACAGT
    AATTCTCAGC AGGGAACATC AAGCATGCTC AAAGATGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GCGATCAAAC CTCAGAGAAG CCTCCTTTCG CACCTGCACC TCCAAGCGCT
    CCACCGGCGC TGCCAGCAGC CGCGGCCTCA GCACATTCCA GCAGCGCCGA ATCGGAGAGC
    ACCAGCGACT CCGACAGCTC CTCGGACTCG GAGAGCGAGA GCAGCTCCAG TGACAGCGAG
    GAGAACGAAC CCCGAGAAGC CCCGGCTCCC GAGCCCGAGC CTCCGACAAC CAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCCGCGG TGCCCACCGA GGCCCCGGGT
    AGCACGGAGC CCCCGGCCCG ACCCCCGGAC ACTAAGGGCA AGGGCGGCGA CAGCGCCACC
    GCGGGTCACG AGCGCCCAGA GTCCAAAGAG CCTCCCCCCA AAGGCTCCAG CAAAGCCCCC
    CGGGTCCCAT CCGAAGGCCC CCACGCGGGC AAGAGGAGCT GTCAGAAGTC CCCTACCCAG
    CAGGAGCCCC CCCAGCGGCA AACCGTGGGA AGCAAACAGC CGAAAAAGCC CATCAAGGCT
    TCCGCCCCCG GGCACGCGGA TGCGCCTGCA GATGCGCGTG CCTGCCTGCA GGTGGAGAGC
    GAGCCCGGAC TTCCTCCCCC GGCCTCCAGG GACCAGCCCT CCAAAGACAA GCCCAAGGTG
    AAGACGAAAG GGCGGCCCCG CGCCGCAGAC AGCCGGGAGC CCAAGCCGGC GGTGCCCACC
    CCCAGCGACC GGAAGAAGCA CCGCAGCGGC CCCCCGAAGG CGCCCCCGAA GGACGCGGCG
    GCAGAGGACC GGAGCCCCGA ACACTTTGCG CTCGTTCCCC TGACCCAGAG CCAGGGCCCT
    GTCCGCGGTG GCAGCGGCGG CGCCGGCGCC AGGACTAGTG GCTGCAGGCT GGCTGTGGTG
    GTCCAGGAGG ACCGCCGCAA AGACAAACCC CCGGTGCCTT CGAGAGACAC CAAGCTGCTC
    TCCCCGCTCA GGGACGCTCC GCCGCCACAA AGCTTGGTGG TGAAGATCAC ACTCGACCTC
    CTGTCTCGGA TACCCCAGCC CCCAGGGAAG GGCAGCCGCC CCAAGAAACC AGAAGACAAA
    CAGCCACCAG CAGGGAAGAA GCCAGATCCT GAGAAGAGAA GCTCAGAAAA CGCCAGCAAG
    TTAGCCAAAA AGAGAAAGGG TGAAGCAGAG AAAGACCACG ATAGCAAGAA AGTCAGGCTG
    GAGAAGGAAG TCAAGTCACA GCCGTTGTCT TCCTCTTCCC ACAAGGAATC TTCCAAATCA
    AAGATGTCCA GGCCCTCCTC TGAGCCCTCA AAGAAGGAAA TGCTTCCCCC TTCGCTCGTG
    TCATCCTCAT CCTCCCAGAA GCCAGCCAAG CCTGCACAGA AGAGGCCACG GCAGGAAGAC
    CCCGGCTGCC AGGACCCCCA AAAAAGTGCC GGTAGTGCCA AGGGCAGCCA CAGAGACCCT
    TCTGCCTCCA AACACAGAAA AGCAGAGGGA AAGGTCTCTG GAAGCTCCAC AGAGCACAAA
    GGATCTTCTG GAGATACTGC AAACCGTTTT CCAGTGCCTT CTTTGCCAAA TGGTAACTCC
    AAACCAGGGA AGCCTCACGT GAAGTTTGAC AAACAACAAG CCGATTTTCA CCTGAAGGAA
    GCCAAAAGGT TGAAGGACAA AGCAGAATTA GTGACGGACA AGGTAGGGAA GGCCTTTAAA
    TACCTGGAGG CTGCCTTGTC CTTCATTGAG TGCGGCGTCG CCCTGGAGTC GGAGAGCCCC
    GCGTCCAAGT CGGCGTACTC CATCTACTCG GAGACCGTGG ACCTCATTAA GTTCATACTG
    TCATTAAAAT CCTTCTCAGA TGCCACAACG CCAACACAGG AGAAGATATT TGCTGTTCTA
    TGCATGCGTT GCCAGTCCAT TTTGAACATG GCAATGTTTC GTTGTAAAAA AGATATGGCG
    ATAAAGTATT CCCGCACGCT TAACGAACAC TTCTTCAAGA CTTCTTCCAA GGTTGCCCAG
    GCACCTTCTC CATGCATCGC AAGCACAGGC ACACCGTCCC CCCTCTCTCC GATCCCTTCT
    CCGGCCAGCT CCATAGGGTC CCAGTCAAGC GCTGGCAGCG TGGGGAGCGG GGCGCTGCCC
    GCCACCATCA GCACCCCCGT CACCATCCAG AACATGACAT CCTCCTACGT CAGCATCACT
    TCCCACGTCC TTACCGCCTT TGACCTGTGG GAACAGGCTG AGGTCCTTAC CAGGAAGAAC
    AAAGAATTCT TTGCTCAGCT CAGCACAAGT GTGTGCGCGC TGGCCCTCAA CAGCAGTTTG
    ATGGATCTGG TGCATTATAC AAGACAGGGT TTCCAGCGGC TAAAGCAAGT AACCAAGACA
    CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178454.3
    CDS176..3841
    Translation

    Target ORF information:

    RefSeq Version NM_001191525.3
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 1 (AFF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191525.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCCC AGTCAAGCTT GTACAATGAA GACAGAAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC TCACCAAGAT AAAGAGGCAT TTTCTGAAAA GATCCCCCTT
    TTTGGAGAGC CATACAAGAC AGAGAAAGGT GATGAGCTAT CCAGTCGGAT ACAGAACATG
    CTGGGAAACT ATGAAGAGAT GAAGGAGTTC CTTAGTAGCA AGTCCCACCC CCAGCGCTTG
    GATGCTTCTG AAAATAGGCT GGGGAAGCCG AGATACCCTT TATTTCCTGA GAAGGGGAGC
    AGCCTTCCAC CCCACTCCTT CCACACCAGT GTCCACCACC AGCCCGTTAA CACTCCTGCC
    TGTGGGCCAC ATCCTGCTGG TAACATCGGC CACCACCCAA AGATGGCGCA GCCAAGAATG
    GAACCAATGC CAAGTCTCCA CGCCAAAAGC TATGGCCCAC CGGACAGCCA GCACCTGACC
    CAAGAGCGCC TCGGTCAGGA GGGGTACGGC TCTAGTCATC ACAAAAAGGG TGACCGCAGG
    GCTGATGGAG ACCACTGTGC TTTGACGACA GACTCTGCTC CGGAGAGGGA ACTTTCACCT
    CTGTTCTCTT TGCCTTCCCC AGTCCCCCCT TTGTCACCTG TACATTCCAA CCAGCAGACT
    GCTCCCAGGA CGCAAGGAAG CGGCAAAGTT CCCAGCAGTA GCAGTAGCAA TAAAGGCTAC
    TGCCTGGCCA AGTCTCCCAA GGACCTAGCG GTAAAGGTGC ACGATAAAGA GACCCCTCAA
    GACAGCACAG GGGCAGTGGC CAGCCTTGGG GTAGCCCCTC CTCAGCCACC TTCTCAGACC
    TTCCCCCCAC CCTCCCTCCC CTCAAAAAGC CTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGACGGTCA AGACCAGGCT CCTAGCGAGT CTCCTGAACT GAAACCCCTG
    CCGGAGGAGT ACCGGCAGCA GACCTTTGAG AAATCAGACT TAAAAGTGCC TGCCAAAGCC
    AAGCTCACGA AGCTGAAAAT GCCTTCTCAG TCAGTCGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCACTCAT GGCCTCCTCC TTTGACAGCA
    ATACACACGC CTAGTACAGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG
    CACATCAGTT CTGCGGTCCC AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACAGT
    AATTCTCAGC AGGGAACATC AAGCATGCTC AAAGATGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GCGATCAAAC CTCAGAGAAG CCTCCTTTCG CACCTGCACC TCCAAGCGCT
    CCACCGGCGC TGCCAGCAGC CGCGGCCTCA GCACATTCCA GCAGCGCCGA ATCGGAGAGC
    ACCAGCGACT CCGACAGCTC CTCGGACTCG GAGAGCGAGA GCAGCTCCAG TGACAGCGAG
    GAGAACGAAC CCCGAGAAGC CCCGGCTCCC GAGCCCGAGC CTCCGACAAC CAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCCGCGG TGCCCACCGA GGCCCCGGGT
    AGCACGGAGC CCCCGGCCCG ACCCCCGGAC ACTAAGGGCA AGGGCGGCGA CAGCGCCACC
    GCGGGTCACG AGCGCCCAGA GTCCAAAGAG CCTCCCCCCA AAGGCTCCAG CAAAGCCCCC
    CGGGTCCCAT CCGAAGGCCC CCACGCGGGC AAGAGGAGCT GTCAGAAGTC CCCTACCCAG
    CAGGAGCCCC CCCAGCGGCA AACCGTGGGA AGCAAACAGC CGAAAAAGCC CATCAAGGCT
    TCCGCCCCCG GGCACGCGGA TGCGCCTGCA GATGCGCGTG CCTGCCTGCA GGTGGAGAGC
    GAGCCCGGAC TTCCTCCCCC GGCCTCCAGG GACCAGCCCT CCAAAGACAA GCCCAAGGTG
    AAGACGAAAG GGCGGCCCCG CGCCGCAGAC AGCCGGGAGC CCAAGCCGGC GGTGCCCACC
    CCCAGCGACC GGAAGAAGCA CCGCAGCGGC CCCCCGAAGG CGCCCCCGAA GGACGCGGCG
    GCAGAGGACC GGAGCCCCGA ACACTTTGCG CTCGTTCCCC TGACCCAGAG CCAGGGCCCT
    GTCCGCGGTG GCAGCGGCGG CGCCGGCGCC AGGACTAGTG GCTGCAGGCT GGCTGTGGTG
    GTCCAGGAGG ACCGCCGCAA AGACAAACCC CCGGTGCCTT CGAGAGACAC CAAGCTGCTC
    TCCCCGCTCA GGGACGCTCC GCCGCCACAA AGCTTGGTGG TGAAGATCAC ACTCGACCTC
    CTGTCTCGGA TACCCCAGCC CCCAGGGAAG GGCAGCCGCC CCAAGAAACC AGAAGACAAA
    CAGCCACCAG CAGGGAAGAA GCCAGATCCT GAGAAGAGAA GCTCAGAAAA CGCCAGCAAG
    TTAGCCAAAA AGAGAAAGGG TGAAGCAGAG AAAGACCACG ATAGCAAGAA AGTCAGGCTG
    GAGAAGGAAG TCAAGTCACA GCCGTTGTCT TCCTCTTCCC ACAAGGAATC TTCCAAATCA
    AAGATGTCCA GGCCCTCCTC TGAGCCCTCA AAGAAGGAAA TGCTTCCCCC TTCGCTCGTG
    TCATCCTCAT CCTCCCAGAA GCCAGCCAAG CCTGCACAGA AGAGGCCACG GCAGGAAGAC
    CCCGGCTGCC AGGACCCCCA AAAAAGTGCC GGTAGTGCCA AGGGCAGCCA CAGAGACCCT
    TCTGCCTCCA AACACAGAAA AGCAGAGGGA AAGGTCTCTG GAAGCTCCAC AGAGCACAAA
    GGATCTTCTG GAGATACTGC AAACCGTTTT CCAGTGCCTT CTTTGCCAAA TGGTAACTCC
    AAACCAGGGA AGCCTCACGT GAAGTTTGAC AAACAACAAG CCGATTTTCA CCTGAAGGAA
    GCCAAAAGGT TGAAGGACAA AGCAGAATTA GTGACGGACA AGGTAGGGAA GGCCTTTAAA
    TACCTGGAGG CTGCCTTGTC CTTCATTGAG TGCGGCGTCG CCCTGGAGTC GGAGAGCCCC
    GCGTCCAAGT CGGCGTACTC CATCTACTCG GAGACCGTGG ACCTCATTAA GTTCATACTG
    TCATTAAAAT CCTTCTCAGA TGCCACAACG CCAACACAGG AGAAGATATT TGCTGTTCTA
    TGCATGCGTT GCCAGTCCAT TTTGAACATG GCAATGTTTC GTTGTAAAAA AGATATGGCG
    ATAAAGTATT CCCGCACGCT TAACGAACAC TTCTTCAAGA CTTCTTCCAA GGTTGCCCAG
    GCACCTTCTC CATGCATCGC AAGCACAGGC ACACCGTCCC CCCTCTCTCC GATCCCTTCT
    CCGGCCAGCT CCATAGGGTC CCAGTCAAGC GCTGGCAGCG TGGGGAGCGG GGCGCTGCCC
    GCCACCATCA GCACCCCCGT CACCATCCAG AACATGACAT CCTCCTACGT CAGCATCACT
    TCCCACGTCC TTACCGCCTT TGACCTGTGG GAACAGGCTG AGGTCCTTAC CAGGAAGAAC
    AAAGAATTCT TTGCTCAGCT CAGCACAAGT GTGTGCGCGC TGGCCCTCAA CAGCAGTTTG
    ATGGATCTGG TGCATTATAC AAGACAGGGT TTCCAGCGGC TAAAGCAAGT AACCAAGACA
    CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235455
    Clone ID Related Accession (Same CDS sequence) XM_010806299.3 , XM_005208211.4
    Accession Version XM_010806299.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806299.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CCGAAAGAGT CAACAGCAGC AGCAACAGCA GCTTGTACAA TGAAGACAGA 
    AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGATAAAGAG
    GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCATACA AGACAGAGAA AGGTGATGAG
    CTATCCAGTC GGATACAGAA CATGCTGGGA AACTATGAAG AGATGAAGGA GTTCCTTAGT
    AGCAAGTCCC ACCCCCAGCG CTTGGATGCT TCTGAAAATA GGCTGGGGAA GCCGAGATAC
    CCTTTATTTC CTGAGAAGGG GAGCAGCCTT CCACCCCACT CCTTCCACAC CAGTGTCCAC
    CACCAGCCCG TTAACACTCC TGCCTGTGGG CCACATCCTG CTGGTAACAT CGGCCACCAC
    CCAAAGATGG CGCAGCCAAG AATGGAACCA ATGCCAAGTC TCCACGCCAA AAGCTATGGC
    CCACCGGACA GCCAGCACCT GACCCAAGAG CGCCTCGGTC AGGAGGGGTA CGGCTCTAGT
    CATCACAAAA AGGGTGACCG CAGGGCTGAT GGAGACCACT GTGCTTTGAC GACAGACTCT
    GCTCCGGAGA GGGAACTTTC ACCTCTGTTC TCTTTGCCTT CCCCAGTCCC CCCTTTGTCA
    CCTGTACATT CCAACCAGCA GACTGCTCCC AGGACGCAAG GAAGCGGCAA AGTTCCCAGC
    AGTAGCAGTA GCAATAAAGG CTACTGCCTG GCCAAGTCTC CCAAGGACCT AGCGGTAAAG
    GTGCACGATA AAGAGACCCC TCAAGACAGC ACAGGGGCAG TGGCCAGCCT TGGGGTAGCC
    CCTCCTCAGC CACCTTCTCA GACCTTCCCC CCACCCTCCC TCCCCTCAAA AAGCCTTGCA
    ATGCAGCAGA AGCCCACGGC TTATGTCCGG CCCATGGACG GTCAAGACCA GGCTCCTAGC
    GAGTCTCCTG AACTGAAACC CCTGCCGGAG GAGTACCGGC AGCAGACCTT TGAGAAATCA
    GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACGAAGCTGA AAATGCCTTC TCAGTCAGTC
    GAGCAGACCT ACTCCAATGA AGTCCATTGT GTTGAAGAGA TTCTGAAGGA AATGACCCAC
    TCATGGCCTC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CAGCTGAGCC CTCCAAATTT
    CCTTTTCCCA CAAAGGATTC TCAGCACATC AGTTCTGCGG TCCCAAACCA AAAACAATAT
    GATACATCTT CAAAAACTCA CAGTAATTCT CAGCAGGGAA CATCCATGCT CAAAGATGAC
    CTTCAGCTCA GTGACAGTGA GGACAGTGAC AGCGATCAAA CCTCAGAGAA GCCTCCTTTC
    GCACCTGCAC CTCCAAGCGC TCCACCGGCG CTGCCAGCAG CCGCGGCCTC AGCACATTCC
    AGCAGCGCCG AATCGGAGAG CACCAGCGAC TCCGACAGCT CCTCGGACTC GGAGAGCGAG
    AGCAGCTCCA GTGACAGCGA GGAGAACGAA CCCCGAGAAG CCCCGGCTCC CGAGCCCGAG
    CCTCCGACAA CCAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAGCCCGCG
    GTGCCCACCG AGGCCCCGGG TAGCACGGAG CCCCCGGCCC GACCCCCGGA CACTAAGGGC
    AAGGGCGGCG ACAGCGCCAC CGCGGGTCAC GAGCGCCCAG AGTCCAAAGA GCCTCCCCCC
    AAAGGCTCCA GCAAAGCCCC CCGGGTCCCA TCCGAAGGCC CCCACGCGGG CAAGAGGAGC
    TGTCAGAAGT CCCCTACCCA GCAGGAGCCC CCCCAGCGGC AAACCGTGGG AAGCAAACAG
    CCGAAAAAGC CCATCAAGGC TTCCGCCCCC GGGCACGCGG ATGCGCCTGC AGATGCGCGT
    GCCTGCCTGC AGGTGGAGAG CGAGCCCGGA CTTCCTCCCC CGGCCTCCAG GGACCAGCCC
    TCCAAAGACA AGCCCAAGGT GAAGACGAAA GGGCGGCCCC GCGCCGCAGA CAGCCGGGAG
    CCCAAGCCGG CGGTGCCCAC CCCCAGCGAC CGGAAGAAGC ACCGCAGCGG CCCCCCGAAG
    GCGCCCCCGA AGGACGCGGC GGCAGAGGAC CGGAGCCCCG AACACTTTGC GCTCGTTCCC
    CTGACCCAGA GCCAGGGCCC TGTCCGCGGT GGCAGCGGCG GCGCCGGCGC CAGGACTAGT
    GGCTGCAGGC TGGCTGTGGT GGTCCAGGAG GACCGCCGCA AAGACAAACC CCCGGTGCCT
    TCGAGAGACA CCAAGCTGCT CTCCCCGCTC AGGGACGCTC CGCCGCCACA AAGCTTGGTG
    GTGAAGATCA CACTCGACCT CCTGTCTCGG ATACCCCAGC CCCCAGGGAA GGGCAGCCGC
    CCCAAGAAAC CAGAAGACAA ACAGCCACCA GCAGGGAAGA AGCCAGATCC TGAGAAGAGA
    AGCTCAGAAA ACGCCAGCAA GTTAGCCAAA AAGAGAAAGG GTGAAGCAGA GAAAGACCAC
    GATAGCAAGA AAGTCAGGCT GGAGAAGGAA GTCAAGTCAC AGCCGTTGTC TTCCTCTTCC
    CACAAGGAAT CTTCCAAATC AAAGATGTCC AGGCCCTCCT CTGAGCCCTC AAAGAAGGAA
    ATGCTTCCCC CTTCGCTCGT GTCATCCTCA TCCTCCCAGA AGCCAGCCAA GCCTGCACAG
    AAGAGGCCAC GGCAGGAAGA CCCCGGCTGC CAGGACCCCC AAAAAAGTGC CGGTAGTGCC
    AAGGGCAGCC ACAGAGACCC TTCTGCCTCC AAACACAGAA AAGCAGAGGG AAAGGTCTCT
    GGAAGCTCCA CAGAGCACAA AGGATCTTCT GGAGATACTG CAAACCGTTT TCCAGTGCCT
    TCTTTGCCAA ATGGTAACTC CAAACCAGGG AAGCCTCACG TGAAGTTTGA CAAACAACAA
    GCCGATTTTC ACCTGAAGGA AGCCAAAAGG TTGAAGGACA AAGCAGAATT AGTGACGGAC
    AAGGTAGGGA AGGCCTTTAA ATACCTGGAG GCTGCCTTGT CCTTCATTGA GTGCGGCGTC
    GCCCTGGAGT CGGAGAGCCC CGCGTCCAAG TCGGCGTACT CCATCTACTC GGAGACCGTG
    GACCTCATTA AGTTCATACT GTCATTAAAA TCCTTCTCAG ATGCCACAAC GCCAACACAG
    GAGAAGATAT TTGCTGTTCT ATGCATGCGT TGCCAGTCCA TTTTGAACAT GGCAATGTTT
    CGTTGTAAAA AAGATATGGC GATAAAGTAT TCCCGCACGC TTAACGAACA CTTCTTCAAG
    ACTTCTTCCA AGGTTGCCCA GGCACCTTCT CCATGCATCG CAAGAAGCAC AGGCACACCG
    TCCCCCCTCT CTCCGATCCC TTCTCCGGCC AGCTCCATAG GGTCCCAGTC AAGCGCTGGC
    AGCGTGGGGA GCGGGGCGCT GCCCGCCACC ATCAGCACCC CCGTCACCAT CCAGAACATG
    ACATCCTCCT ACGTCAGCAT CACTTCCCAC GTCCTTACCG CCTTTGACCT GTGGGAACAG
    GCTGAGGTCC TTACCAGGAA GAACAAAGAA TTCTTTGCTC AGCTCAGCAC AAGTGTGTGC
    GCGCTGGCCC TCAACAGCAG TTTGATGGAT CTGGTGCATT ATACAAGACA GGGTTTCCAG
    CGGCTAAAGC AAGTAACCAA GACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804601.2
    CDS183..3872
    Translation

    Target ORF information:

    RefSeq Version XM_010806299.3
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806299.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CCGAAAGAGT CAACAGCAGC AGCAACAGCA GCTTGTACAA TGAAGACAGA 
    AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGATAAAGAG
    GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCATACA AGACAGAGAA AGGTGATGAG
    CTATCCAGTC GGATACAGAA CATGCTGGGA AACTATGAAG AGATGAAGGA GTTCCTTAGT
    AGCAAGTCCC ACCCCCAGCG CTTGGATGCT TCTGAAAATA GGCTGGGGAA GCCGAGATAC
    CCTTTATTTC CTGAGAAGGG GAGCAGCCTT CCACCCCACT CCTTCCACAC CAGTGTCCAC
    CACCAGCCCG TTAACACTCC TGCCTGTGGG CCACATCCTG CTGGTAACAT CGGCCACCAC
    CCAAAGATGG CGCAGCCAAG AATGGAACCA ATGCCAAGTC TCCACGCCAA AAGCTATGGC
    CCACCGGACA GCCAGCACCT GACCCAAGAG CGCCTCGGTC AGGAGGGGTA CGGCTCTAGT
    CATCACAAAA AGGGTGACCG CAGGGCTGAT GGAGACCACT GTGCTTTGAC GACAGACTCT
    GCTCCGGAGA GGGAACTTTC ACCTCTGTTC TCTTTGCCTT CCCCAGTCCC CCCTTTGTCA
    CCTGTACATT CCAACCAGCA GACTGCTCCC AGGACGCAAG GAAGCGGCAA AGTTCCCAGC
    AGTAGCAGTA GCAATAAAGG CTACTGCCTG GCCAAGTCTC CCAAGGACCT AGCGGTAAAG
    GTGCACGATA AAGAGACCCC TCAAGACAGC ACAGGGGCAG TGGCCAGCCT TGGGGTAGCC
    CCTCCTCAGC CACCTTCTCA GACCTTCCCC CCACCCTCCC TCCCCTCAAA AAGCCTTGCA
    ATGCAGCAGA AGCCCACGGC TTATGTCCGG CCCATGGACG GTCAAGACCA GGCTCCTAGC
    GAGTCTCCTG AACTGAAACC CCTGCCGGAG GAGTACCGGC AGCAGACCTT TGAGAAATCA
    GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACGAAGCTGA AAATGCCTTC TCAGTCAGTC
    GAGCAGACCT ACTCCAATGA AGTCCATTGT GTTGAAGAGA TTCTGAAGGA AATGACCCAC
    TCATGGCCTC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CAGCTGAGCC CTCCAAATTT
    CCTTTTCCCA CAAAGGATTC TCAGCACATC AGTTCTGCGG TCCCAAACCA AAAACAATAT
    GATACATCTT CAAAAACTCA CAGTAATTCT CAGCAGGGAA CATCCATGCT CAAAGATGAC
    CTTCAGCTCA GTGACAGTGA GGACAGTGAC AGCGATCAAA CCTCAGAGAA GCCTCCTTTC
    GCACCTGCAC CTCCAAGCGC TCCACCGGCG CTGCCAGCAG CCGCGGCCTC AGCACATTCC
    AGCAGCGCCG AATCGGAGAG CACCAGCGAC TCCGACAGCT CCTCGGACTC GGAGAGCGAG
    AGCAGCTCCA GTGACAGCGA GGAGAACGAA CCCCGAGAAG CCCCGGCTCC CGAGCCCGAG
    CCTCCGACAA CCAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAGCCCGCG
    GTGCCCACCG AGGCCCCGGG TAGCACGGAG CCCCCGGCCC GACCCCCGGA CACTAAGGGC
    AAGGGCGGCG ACAGCGCCAC CGCGGGTCAC GAGCGCCCAG AGTCCAAAGA GCCTCCCCCC
    AAAGGCTCCA GCAAAGCCCC CCGGGTCCCA TCCGAAGGCC CCCACGCGGG CAAGAGGAGC
    TGTCAGAAGT CCCCTACCCA GCAGGAGCCC CCCCAGCGGC AAACCGTGGG AAGCAAACAG
    CCGAAAAAGC CCATCAAGGC TTCCGCCCCC GGGCACGCGG ATGCGCCTGC AGATGCGCGT
    GCCTGCCTGC AGGTGGAGAG CGAGCCCGGA CTTCCTCCCC CGGCCTCCAG GGACCAGCCC
    TCCAAAGACA AGCCCAAGGT GAAGACGAAA GGGCGGCCCC GCGCCGCAGA CAGCCGGGAG
    CCCAAGCCGG CGGTGCCCAC CCCCAGCGAC CGGAAGAAGC ACCGCAGCGG CCCCCCGAAG
    GCGCCCCCGA AGGACGCGGC GGCAGAGGAC CGGAGCCCCG AACACTTTGC GCTCGTTCCC
    CTGACCCAGA GCCAGGGCCC TGTCCGCGGT GGCAGCGGCG GCGCCGGCGC CAGGACTAGT
    GGCTGCAGGC TGGCTGTGGT GGTCCAGGAG GACCGCCGCA AAGACAAACC CCCGGTGCCT
    TCGAGAGACA CCAAGCTGCT CTCCCCGCTC AGGGACGCTC CGCCGCCACA AAGCTTGGTG
    GTGAAGATCA CACTCGACCT CCTGTCTCGG ATACCCCAGC CCCCAGGGAA GGGCAGCCGC
    CCCAAGAAAC CAGAAGACAA ACAGCCACCA GCAGGGAAGA AGCCAGATCC TGAGAAGAGA
    AGCTCAGAAA ACGCCAGCAA GTTAGCCAAA AAGAGAAAGG GTGAAGCAGA GAAAGACCAC
    GATAGCAAGA AAGTCAGGCT GGAGAAGGAA GTCAAGTCAC AGCCGTTGTC TTCCTCTTCC
    CACAAGGAAT CTTCCAAATC AAAGATGTCC AGGCCCTCCT CTGAGCCCTC AAAGAAGGAA
    ATGCTTCCCC CTTCGCTCGT GTCATCCTCA TCCTCCCAGA AGCCAGCCAA GCCTGCACAG
    AAGAGGCCAC GGCAGGAAGA CCCCGGCTGC CAGGACCCCC AAAAAAGTGC CGGTAGTGCC
    AAGGGCAGCC ACAGAGACCC TTCTGCCTCC AAACACAGAA AAGCAGAGGG AAAGGTCTCT
    GGAAGCTCCA CAGAGCACAA AGGATCTTCT GGAGATACTG CAAACCGTTT TCCAGTGCCT
    TCTTTGCCAA ATGGTAACTC CAAACCAGGG AAGCCTCACG TGAAGTTTGA CAAACAACAA
    GCCGATTTTC ACCTGAAGGA AGCCAAAAGG TTGAAGGACA AAGCAGAATT AGTGACGGAC
    AAGGTAGGGA AGGCCTTTAA ATACCTGGAG GCTGCCTTGT CCTTCATTGA GTGCGGCGTC
    GCCCTGGAGT CGGAGAGCCC CGCGTCCAAG TCGGCGTACT CCATCTACTC GGAGACCGTG
    GACCTCATTA AGTTCATACT GTCATTAAAA TCCTTCTCAG ATGCCACAAC GCCAACACAG
    GAGAAGATAT TTGCTGTTCT ATGCATGCGT TGCCAGTCCA TTTTGAACAT GGCAATGTTT
    CGTTGTAAAA AAGATATGGC GATAAAGTAT TCCCGCACGC TTAACGAACA CTTCTTCAAG
    ACTTCTTCCA AGGTTGCCCA GGCACCTTCT CCATGCATCG CAAGAAGCAC AGGCACACCG
    TCCCCCCTCT CTCCGATCCC TTCTCCGGCC AGCTCCATAG GGTCCCAGTC AAGCGCTGGC
    AGCGTGGGGA GCGGGGCGCT GCCCGCCACC ATCAGCACCC CCGTCACCAT CCAGAACATG
    ACATCCTCCT ACGTCAGCAT CACTTCCCAC GTCCTTACCG CCTTTGACCT GTGGGAACAG
    GCTGAGGTCC TTACCAGGAA GAACAAAGAA TTCTTTGCTC AGCTCAGCAC AAGTGTGTGC
    GCGCTGGCCC TCAACAGCAG TTTGATGGAT CTGGTGCATT ATACAAGACA GGGTTTCCAG
    CGGCTAAAGC AAGTAACCAA GACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235455
    Clone ID Related Accession (Same CDS sequence) XM_010806299.3 , XM_005208211.4
    Accession Version XM_005208211.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208211.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CCGAAAGAGT CAACAGCAGC AGCAACAGCA GCTTGTACAA TGAAGACAGA 
    AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGATAAAGAG
    GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCATACA AGACAGAGAA AGGTGATGAG
    CTATCCAGTC GGATACAGAA CATGCTGGGA AACTATGAAG AGATGAAGGA GTTCCTTAGT
    AGCAAGTCCC ACCCCCAGCG CTTGGATGCT TCTGAAAATA GGCTGGGGAA GCCGAGATAC
    CCTTTATTTC CTGAGAAGGG GAGCAGCCTT CCACCCCACT CCTTCCACAC CAGTGTCCAC
    CACCAGCCCG TTAACACTCC TGCCTGTGGG CCACATCCTG CTGGTAACAT CGGCCACCAC
    CCAAAGATGG CGCAGCCAAG AATGGAACCA ATGCCAAGTC TCCACGCCAA AAGCTATGGC
    CCACCGGACA GCCAGCACCT GACCCAAGAG CGCCTCGGTC AGGAGGGGTA CGGCTCTAGT
    CATCACAAAA AGGGTGACCG CAGGGCTGAT GGAGACCACT GTGCTTTGAC GACAGACTCT
    GCTCCGGAGA GGGAACTTTC ACCTCTGTTC TCTTTGCCTT CCCCAGTCCC CCCTTTGTCA
    CCTGTACATT CCAACCAGCA GACTGCTCCC AGGACGCAAG GAAGCGGCAA AGTTCCCAGC
    AGTAGCAGTA GCAATAAAGG CTACTGCCTG GCCAAGTCTC CCAAGGACCT AGCGGTAAAG
    GTGCACGATA AAGAGACCCC TCAAGACAGC ACAGGGGCAG TGGCCAGCCT TGGGGTAGCC
    CCTCCTCAGC CACCTTCTCA GACCTTCCCC CCACCCTCCC TCCCCTCAAA AAGCCTTGCA
    ATGCAGCAGA AGCCCACGGC TTATGTCCGG CCCATGGACG GTCAAGACCA GGCTCCTAGC
    GAGTCTCCTG AACTGAAACC CCTGCCGGAG GAGTACCGGC AGCAGACCTT TGAGAAATCA
    GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACGAAGCTGA AAATGCCTTC TCAGTCAGTC
    GAGCAGACCT ACTCCAATGA AGTCCATTGT GTTGAAGAGA TTCTGAAGGA AATGACCCAC
    TCATGGCCTC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CAGCTGAGCC CTCCAAATTT
    CCTTTTCCCA CAAAGGATTC TCAGCACATC AGTTCTGCGG TCCCAAACCA AAAACAATAT
    GATACATCTT CAAAAACTCA CAGTAATTCT CAGCAGGGAA CATCCATGCT CAAAGATGAC
    CTTCAGCTCA GTGACAGTGA GGACAGTGAC AGCGATCAAA CCTCAGAGAA GCCTCCTTTC
    GCACCTGCAC CTCCAAGCGC TCCACCGGCG CTGCCAGCAG CCGCGGCCTC AGCACATTCC
    AGCAGCGCCG AATCGGAGAG CACCAGCGAC TCCGACAGCT CCTCGGACTC GGAGAGCGAG
    AGCAGCTCCA GTGACAGCGA GGAGAACGAA CCCCGAGAAG CCCCGGCTCC CGAGCCCGAG
    CCTCCGACAA CCAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAGCCCGCG
    GTGCCCACCG AGGCCCCGGG TAGCACGGAG CCCCCGGCCC GACCCCCGGA CACTAAGGGC
    AAGGGCGGCG ACAGCGCCAC CGCGGGTCAC GAGCGCCCAG AGTCCAAAGA GCCTCCCCCC
    AAAGGCTCCA GCAAAGCCCC CCGGGTCCCA TCCGAAGGCC CCCACGCGGG CAAGAGGAGC
    TGTCAGAAGT CCCCTACCCA GCAGGAGCCC CCCCAGCGGC AAACCGTGGG AAGCAAACAG
    CCGAAAAAGC CCATCAAGGC TTCCGCCCCC GGGCACGCGG ATGCGCCTGC AGATGCGCGT
    GCCTGCCTGC AGGTGGAGAG CGAGCCCGGA CTTCCTCCCC CGGCCTCCAG GGACCAGCCC
    TCCAAAGACA AGCCCAAGGT GAAGACGAAA GGGCGGCCCC GCGCCGCAGA CAGCCGGGAG
    CCCAAGCCGG CGGTGCCCAC CCCCAGCGAC CGGAAGAAGC ACCGCAGCGG CCCCCCGAAG
    GCGCCCCCGA AGGACGCGGC GGCAGAGGAC CGGAGCCCCG AACACTTTGC GCTCGTTCCC
    CTGACCCAGA GCCAGGGCCC TGTCCGCGGT GGCAGCGGCG GCGCCGGCGC CAGGACTAGT
    GGCTGCAGGC TGGCTGTGGT GGTCCAGGAG GACCGCCGCA AAGACAAACC CCCGGTGCCT
    TCGAGAGACA CCAAGCTGCT CTCCCCGCTC AGGGACGCTC CGCCGCCACA AAGCTTGGTG
    GTGAAGATCA CACTCGACCT CCTGTCTCGG ATACCCCAGC CCCCAGGGAA GGGCAGCCGC
    CCCAAGAAAC CAGAAGACAA ACAGCCACCA GCAGGGAAGA AGCCAGATCC TGAGAAGAGA
    AGCTCAGAAA ACGCCAGCAA GTTAGCCAAA AAGAGAAAGG GTGAAGCAGA GAAAGACCAC
    GATAGCAAGA AAGTCAGGCT GGAGAAGGAA GTCAAGTCAC AGCCGTTGTC TTCCTCTTCC
    CACAAGGAAT CTTCCAAATC AAAGATGTCC AGGCCCTCCT CTGAGCCCTC AAAGAAGGAA
    ATGCTTCCCC CTTCGCTCGT GTCATCCTCA TCCTCCCAGA AGCCAGCCAA GCCTGCACAG
    AAGAGGCCAC GGCAGGAAGA CCCCGGCTGC CAGGACCCCC AAAAAAGTGC CGGTAGTGCC
    AAGGGCAGCC ACAGAGACCC TTCTGCCTCC AAACACAGAA AAGCAGAGGG AAAGGTCTCT
    GGAAGCTCCA CAGAGCACAA AGGATCTTCT GGAGATACTG CAAACCGTTT TCCAGTGCCT
    TCTTTGCCAA ATGGTAACTC CAAACCAGGG AAGCCTCACG TGAAGTTTGA CAAACAACAA
    GCCGATTTTC ACCTGAAGGA AGCCAAAAGG TTGAAGGACA AAGCAGAATT AGTGACGGAC
    AAGGTAGGGA AGGCCTTTAA ATACCTGGAG GCTGCCTTGT CCTTCATTGA GTGCGGCGTC
    GCCCTGGAGT CGGAGAGCCC CGCGTCCAAG TCGGCGTACT CCATCTACTC GGAGACCGTG
    GACCTCATTA AGTTCATACT GTCATTAAAA TCCTTCTCAG ATGCCACAAC GCCAACACAG
    GAGAAGATAT TTGCTGTTCT ATGCATGCGT TGCCAGTCCA TTTTGAACAT GGCAATGTTT
    CGTTGTAAAA AAGATATGGC GATAAAGTAT TCCCGCACGC TTAACGAACA CTTCTTCAAG
    ACTTCTTCCA AGGTTGCCCA GGCACCTTCT CCATGCATCG CAAGAAGCAC AGGCACACCG
    TCCCCCCTCT CTCCGATCCC TTCTCCGGCC AGCTCCATAG GGTCCCAGTC AAGCGCTGGC
    AGCGTGGGGA GCGGGGCGCT GCCCGCCACC ATCAGCACCC CCGTCACCAT CCAGAACATG
    ACATCCTCCT ACGTCAGCAT CACTTCCCAC GTCCTTACCG CCTTTGACCT GTGGGAACAG
    GCTGAGGTCC TTACCAGGAA GAACAAAGAA TTCTTTGCTC AGCTCAGCAC AAGTGTGTGC
    GCGCTGGCCC TCAACAGCAG TTTGATGGAT CTGGTGCATT ATACAAGACA GGGTTTCCAG
    CGGCTAAAGC AAGTAACCAA GACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208268.2
    CDS171..3860
    Translation

    Target ORF information:

    RefSeq Version XM_005208211.4
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208211.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CCGAAAGAGT CAACAGCAGC AGCAACAGCA GCTTGTACAA TGAAGACAGA 
    AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGATAAAGAG
    GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCATACA AGACAGAGAA AGGTGATGAG
    CTATCCAGTC GGATACAGAA CATGCTGGGA AACTATGAAG AGATGAAGGA GTTCCTTAGT
    AGCAAGTCCC ACCCCCAGCG CTTGGATGCT TCTGAAAATA GGCTGGGGAA GCCGAGATAC
    CCTTTATTTC CTGAGAAGGG GAGCAGCCTT CCACCCCACT CCTTCCACAC CAGTGTCCAC
    CACCAGCCCG TTAACACTCC TGCCTGTGGG CCACATCCTG CTGGTAACAT CGGCCACCAC
    CCAAAGATGG CGCAGCCAAG AATGGAACCA ATGCCAAGTC TCCACGCCAA AAGCTATGGC
    CCACCGGACA GCCAGCACCT GACCCAAGAG CGCCTCGGTC AGGAGGGGTA CGGCTCTAGT
    CATCACAAAA AGGGTGACCG CAGGGCTGAT GGAGACCACT GTGCTTTGAC GACAGACTCT
    GCTCCGGAGA GGGAACTTTC ACCTCTGTTC TCTTTGCCTT CCCCAGTCCC CCCTTTGTCA
    CCTGTACATT CCAACCAGCA GACTGCTCCC AGGACGCAAG GAAGCGGCAA AGTTCCCAGC
    AGTAGCAGTA GCAATAAAGG CTACTGCCTG GCCAAGTCTC CCAAGGACCT AGCGGTAAAG
    GTGCACGATA AAGAGACCCC TCAAGACAGC ACAGGGGCAG TGGCCAGCCT TGGGGTAGCC
    CCTCCTCAGC CACCTTCTCA GACCTTCCCC CCACCCTCCC TCCCCTCAAA AAGCCTTGCA
    ATGCAGCAGA AGCCCACGGC TTATGTCCGG CCCATGGACG GTCAAGACCA GGCTCCTAGC
    GAGTCTCCTG AACTGAAACC CCTGCCGGAG GAGTACCGGC AGCAGACCTT TGAGAAATCA
    GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACGAAGCTGA AAATGCCTTC TCAGTCAGTC
    GAGCAGACCT ACTCCAATGA AGTCCATTGT GTTGAAGAGA TTCTGAAGGA AATGACCCAC
    TCATGGCCTC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CAGCTGAGCC CTCCAAATTT
    CCTTTTCCCA CAAAGGATTC TCAGCACATC AGTTCTGCGG TCCCAAACCA AAAACAATAT
    GATACATCTT CAAAAACTCA CAGTAATTCT CAGCAGGGAA CATCCATGCT CAAAGATGAC
    CTTCAGCTCA GTGACAGTGA GGACAGTGAC AGCGATCAAA CCTCAGAGAA GCCTCCTTTC
    GCACCTGCAC CTCCAAGCGC TCCACCGGCG CTGCCAGCAG CCGCGGCCTC AGCACATTCC
    AGCAGCGCCG AATCGGAGAG CACCAGCGAC TCCGACAGCT CCTCGGACTC GGAGAGCGAG
    AGCAGCTCCA GTGACAGCGA GGAGAACGAA CCCCGAGAAG CCCCGGCTCC CGAGCCCGAG
    CCTCCGACAA CCAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAGCCCGCG
    GTGCCCACCG AGGCCCCGGG TAGCACGGAG CCCCCGGCCC GACCCCCGGA CACTAAGGGC
    AAGGGCGGCG ACAGCGCCAC CGCGGGTCAC GAGCGCCCAG AGTCCAAAGA GCCTCCCCCC
    AAAGGCTCCA GCAAAGCCCC CCGGGTCCCA TCCGAAGGCC CCCACGCGGG CAAGAGGAGC
    TGTCAGAAGT CCCCTACCCA GCAGGAGCCC CCCCAGCGGC AAACCGTGGG AAGCAAACAG
    CCGAAAAAGC CCATCAAGGC TTCCGCCCCC GGGCACGCGG ATGCGCCTGC AGATGCGCGT
    GCCTGCCTGC AGGTGGAGAG CGAGCCCGGA CTTCCTCCCC CGGCCTCCAG GGACCAGCCC
    TCCAAAGACA AGCCCAAGGT GAAGACGAAA GGGCGGCCCC GCGCCGCAGA CAGCCGGGAG
    CCCAAGCCGG CGGTGCCCAC CCCCAGCGAC CGGAAGAAGC ACCGCAGCGG CCCCCCGAAG
    GCGCCCCCGA AGGACGCGGC GGCAGAGGAC CGGAGCCCCG AACACTTTGC GCTCGTTCCC
    CTGACCCAGA GCCAGGGCCC TGTCCGCGGT GGCAGCGGCG GCGCCGGCGC CAGGACTAGT
    GGCTGCAGGC TGGCTGTGGT GGTCCAGGAG GACCGCCGCA AAGACAAACC CCCGGTGCCT
    TCGAGAGACA CCAAGCTGCT CTCCCCGCTC AGGGACGCTC CGCCGCCACA AAGCTTGGTG
    GTGAAGATCA CACTCGACCT CCTGTCTCGG ATACCCCAGC CCCCAGGGAA GGGCAGCCGC
    CCCAAGAAAC CAGAAGACAA ACAGCCACCA GCAGGGAAGA AGCCAGATCC TGAGAAGAGA
    AGCTCAGAAA ACGCCAGCAA GTTAGCCAAA AAGAGAAAGG GTGAAGCAGA GAAAGACCAC
    GATAGCAAGA AAGTCAGGCT GGAGAAGGAA GTCAAGTCAC AGCCGTTGTC TTCCTCTTCC
    CACAAGGAAT CTTCCAAATC AAAGATGTCC AGGCCCTCCT CTGAGCCCTC AAAGAAGGAA
    ATGCTTCCCC CTTCGCTCGT GTCATCCTCA TCCTCCCAGA AGCCAGCCAA GCCTGCACAG
    AAGAGGCCAC GGCAGGAAGA CCCCGGCTGC CAGGACCCCC AAAAAAGTGC CGGTAGTGCC
    AAGGGCAGCC ACAGAGACCC TTCTGCCTCC AAACACAGAA AAGCAGAGGG AAAGGTCTCT
    GGAAGCTCCA CAGAGCACAA AGGATCTTCT GGAGATACTG CAAACCGTTT TCCAGTGCCT
    TCTTTGCCAA ATGGTAACTC CAAACCAGGG AAGCCTCACG TGAAGTTTGA CAAACAACAA
    GCCGATTTTC ACCTGAAGGA AGCCAAAAGG TTGAAGGACA AAGCAGAATT AGTGACGGAC
    AAGGTAGGGA AGGCCTTTAA ATACCTGGAG GCTGCCTTGT CCTTCATTGA GTGCGGCGTC
    GCCCTGGAGT CGGAGAGCCC CGCGTCCAAG TCGGCGTACT CCATCTACTC GGAGACCGTG
    GACCTCATTA AGTTCATACT GTCATTAAAA TCCTTCTCAG ATGCCACAAC GCCAACACAG
    GAGAAGATAT TTGCTGTTCT ATGCATGCGT TGCCAGTCCA TTTTGAACAT GGCAATGTTT
    CGTTGTAAAA AAGATATGGC GATAAAGTAT TCCCGCACGC TTAACGAACA CTTCTTCAAG
    ACTTCTTCCA AGGTTGCCCA GGCACCTTCT CCATGCATCG CAAGAAGCAC AGGCACACCG
    TCCCCCCTCT CTCCGATCCC TTCTCCGGCC AGCTCCATAG GGTCCCAGTC AAGCGCTGGC
    AGCGTGGGGA GCGGGGCGCT GCCCGCCACC ATCAGCACCC CCGTCACCAT CCAGAACATG
    ACATCCTCCT ACGTCAGCAT CACTTCCCAC GTCCTTACCG CCTTTGACCT GTGGGAACAG
    GCTGAGGTCC TTACCAGGAA GAACAAAGAA TTCTTTGCTC AGCTCAGCAC AAGTGTGTGC
    GCGCTGGCCC TCAACAGCAG TTTGATGGAT CTGGTGCATT ATACAAGACA GGGTTTCCAG
    CGGCTAAAGC AAGTAACCAA GACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235456
    Clone ID Related Accession (Same CDS sequence) XM_024993466.1 , XM_010806300.3
    Accession Version XM_024993466.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3687bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CCGAAAGAGT CAACAGCAGC AGCAACAGCT TGTACAATGA AGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CTCACCAAGA TAAAGAGGCA
    TTTTCTGAAA AGATCCCCCT TTTTGGAGAG CCATACAAGA CAGAGAAAGG TGATGAGCTA
    TCCAGTCGGA TACAGAACAT GCTGGGAAAC TATGAAGAGA TGAAGGAGTT CCTTAGTAGC
    AAGTCCCACC CCCAGCGCTT GGATGCTTCT GAAAATAGGC TGGGGAAGCC GAGATACCCT
    TTATTTCCTG AGAAGGGGAG CAGCCTTCCA CCCCACTCCT TCCACACCAG TGTCCACCAC
    CAGCCCGTTA ACACTCCTGC CTGTGGGCCA CATCCTGCTG GTAACATCGG CCACCACCCA
    AAGATGGCGC AGCCAAGAAT GGAACCAATG CCAAGTCTCC ACGCCAAAAG CTATGGCCCA
    CCGGACAGCC AGCACCTGAC CCAAGAGCGC CTCGGTCAGG AGGGGTACGG CTCTAGTCAT
    CACAAAAAGG GTGACCGCAG GGCTGATGGA GACCACTGTG CTTTGACGAC AGACTCTGCT
    CCGGAGAGGG AACTTTCACC TCTGTTCTCT TTGCCTTCCC CAGTCCCCCC TTTGTCACCT
    GTACATTCCA ACCAGCAGAC TGCTCCCAGG ACGCAAGGAA GCGGCAAAGT TCCCAGCAGT
    AGCAGTAGCA ATAAAGGCTA CTGCCTGGCC AAGTCTCCCA AGGACCTAGC GGTAAAGGTG
    CACGATAAAG AGACCCCTCA AGACAGCACA GGGGCAGTGG CCAGCCTTGG GGTAGCCCCT
    CCTCAGCCAC CTTCTCAGAC CTTCCCCCCA CCCTCCCTCC CCTCAAAAAG CCTTGCAATG
    CAGCAGAAGC CCACGGCTTA TGTCCGGCCC ATGGACGGTC AAGACCAGGC TCCTAGCGAG
    TCTCCTGAAC TGAAACCCCT GCCGGAGGAG TACCGGCAGC AGACCTTTGA GAAATCAGAC
    TTAAAAGTGC CTGCCAAAGC CAAGCTCACG AAGCTGAAAA TGCCTTCTCA GTCAGTCGAG
    CAGACCTACT CCAATGAAGT CCATTGTGTT GAAGAGATTC TGAAGGAAAT GACCCACTCA
    TGGCCTCCTC CTTTGACAGC AATACACACG CCTAGTACAG CTGAGCCCTC CAAATTTCCT
    TTTCCCACAA AGGATTCTCA GCACATCAGT TCTGCGGTCC CAAACCAAAA ACAATATGAT
    ACATCTTCAA AAACTCACAG TAATTCTCAG CAGGGAACAT CCATGCTCAA AGATGACCTT
    CAGCTCAGTG ACAGTGAGGA CAGTGACAGC GATCAAACCT CAGAGAAGCC TCCTTTCGCA
    CCTGCACCTC CAAGCGCTCC ACCGGCGCTG CCAGCAGCCG CGGCCTCAGC ACATTCCAGC
    AGCGCCGAAT CGGAGAGCAC CAGCGACTCC GACAGCTCCT CGGACTCGGA GAGCGAGAGC
    AGCTCCAGTG ACAGCGAGGA GAACGAACCC CGAGAAGCCC CGGCTCCCGA GCCCGAGCCT
    CCGACAACCA ACAAATGGCA GCTGGACAAC TGGCTGACCA AAGTCAGCCA GCCCGCGGTG
    CCCACCGAGG CCCCGGGTAG CACGGAGCCC CCGGCCCGAC CCCCGGACAC TAAGGGCAAG
    GGCGGCGACA GCGCCACCGC GGGTCACGAG CGCCCAGAGT CCAAAGAGCC TCCCCCCAAA
    GGCTCCAGCA AAGCCCCCCG GGTCCCATCC GAAGGCCCCC ACGCGGGCAA GAGGAGCTGT
    CAGAAGTCCC CTACCCAGCA GGAGCCCCCC CAGCGGCAAA CCGTGGGAAG CAAACAGCCG
    AAAAAGCCCA TCAAGGCTTC CGCCCCCGGG CACGCGGATG CGCCTGCAGA TGCGCGTGCC
    TGCCTGCAGG TGGAGAGCGA GCCCGGACTT CCTCCCCCGG CCTCCAGGGA CCAGCCCTCC
    AAAGACAAGC CCAAGGTGAA GACGAAAGGG CGGCCCCGCG CCGCAGACAG CCGGGAGCCC
    AAGCCGGCGG TGCCCACCCC CAGCGACCGG AAGAAGCACC GCAGCGGCCC CCCGAAGGCG
    CCCCCGAAGG ACGCGGCGGC AGAGGACCGG AGCCCCGAAC ACTTTGCGCT CGTTCCCCTG
    ACCCAGAGCC AGGGCCCTGT CCGCGGTGGC AGCGGCGGCG CCGGCGCCAG GACTAGTGGC
    TGCAGGCTGG CTGTGGTGGT CCAGGAGGAC CGCCGCAAAG ACAAACCCCC GGTGCCTTCG
    AGAGACACCA AGCTGCTCTC CCCGCTCAGG GACGCTCCGC CGCCACAAAG CTTGGTGGTG
    AAGATCACAC TCGACCTCCT GTCTCGGATA CCCCAGCCCC CAGGGAAGGG CAGCCGCCCC
    AAGAAACCAG AAGACAAACA GCCACCAGCA GGGAAGAAGC CAGATCCTGA GAAGAGAAGC
    TCAGAAAACG CCAGCAAGTT AGCCAAAAAG AGAAAGGGTG AAGCAGAGAA AGACCACGAT
    AGCAAGAAAG TCAGGCTGGA GAAGGAAGTC AAGTCACAGC CGTTGTCTTC CTCTTCCCAC
    AAGGAATCTT CCAAATCAAA GATGTCCAGG CCCTCCTCTG AGCCCTCAAA GAAGGAAATG
    CTTCCCCCTT CGCTCGTGTC ATCCTCATCC TCCCAGAAGC CAGCCAAGCC TGCACAGAAG
    AGGCCACGGC AGGAAGACCC CGGCTGCCAG GACCCCCAAA AAAGTGCCGG TAGTGCCAAG
    GGCAGCCACA GAGACCCTTC TGCCTCCAAA CACAGAAAAG CAGAGGGAAA GGTCTCTGGA
    AGCTCCACAG AGCACAAAGG ATCTTCTGGA GATACTGCAA ACCGTTTTCC AGTGCCTTCT
    TTGCCAAATG GTAACTCCAA ACCAGGGAAG CCTCACGTGA AGTTTGACAA ACAACAAGCC
    GATTTTCACC TGAAGGAAGC CAAAAGGTTG AAGGACAAAG CAGAATTAGT GACGGACAAG
    GTAGGGAAGG CCTTTAAATA CCTGGAGGCT GCCTTGTCCT TCATTGAGTG CGGCGTCGCC
    CTGGAGTCGG AGAGCCCCGC GTCCAAGTCG GCGTACTCCA TCTACTCGGA GACCGTGGAC
    CTCATTAAGT TCATACTGTC ATTAAAATCC TTCTCAGATG CCACAACGCC AACACAGGAG
    AAGATATTTG CTGTTCTATG CATGCGTTGC CAGTCCATTT TGAACATGGC AATGTTTCGT
    TGTAAAAAAG ATATGGCGAT AAAGTATTCC CGCACGCTTA ACGAACACTT CTTCAAGACT
    TCTTCCAAGG TTGCCCAGGC ACCTTCTCCA TGCATCGCAA GAAGCACAGG CACACCGTCC
    CCCCTCTCTC CGATCCCTTC TCCGGCCAGC TCCATAGGGT CCCAGTCAAG CGCTGGCAGC
    GTGGGGAGCG GGGCGCTGCC CGCCACCATC AGCACCCCCG TCACCATCCA GAACATGACA
    TCCTCCTACG TCAGCATCAC TTCCCACGTC CTTACCGCCT TTGACCTGTG GGAACAGGCT
    GAGGTCCTTA CCAGGAAGAA CAAAGAATTC TTTGCTCAGC TCAGCACAAG TGTGTGCGCG
    CTGGCCCTCA ACAGCAGTTT GATGGATCTG GTGCATTATA CAAGACAGGG TTTCCAGCGG
    CTAAAGCAAG TAACCAAGAC ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849234.1
    CDS560..4246
    Translation

    Target ORF information:

    RefSeq Version XM_024993466.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993466.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CCGAAAGAGT CAACAGCAGC AGCAACAGCT TGTACAATGA AGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CTCACCAAGA TAAAGAGGCA
    TTTTCTGAAA AGATCCCCCT TTTTGGAGAG CCATACAAGA CAGAGAAAGG TGATGAGCTA
    TCCAGTCGGA TACAGAACAT GCTGGGAAAC TATGAAGAGA TGAAGGAGTT CCTTAGTAGC
    AAGTCCCACC CCCAGCGCTT GGATGCTTCT GAAAATAGGC TGGGGAAGCC GAGATACCCT
    TTATTTCCTG AGAAGGGGAG CAGCCTTCCA CCCCACTCCT TCCACACCAG TGTCCACCAC
    CAGCCCGTTA ACACTCCTGC CTGTGGGCCA CATCCTGCTG GTAACATCGG CCACCACCCA
    AAGATGGCGC AGCCAAGAAT GGAACCAATG CCAAGTCTCC ACGCCAAAAG CTATGGCCCA
    CCGGACAGCC AGCACCTGAC CCAAGAGCGC CTCGGTCAGG AGGGGTACGG CTCTAGTCAT
    CACAAAAAGG GTGACCGCAG GGCTGATGGA GACCACTGTG CTTTGACGAC AGACTCTGCT
    CCGGAGAGGG AACTTTCACC TCTGTTCTCT TTGCCTTCCC CAGTCCCCCC TTTGTCACCT
    GTACATTCCA ACCAGCAGAC TGCTCCCAGG ACGCAAGGAA GCGGCAAAGT TCCCAGCAGT
    AGCAGTAGCA ATAAAGGCTA CTGCCTGGCC AAGTCTCCCA AGGACCTAGC GGTAAAGGTG
    CACGATAAAG AGACCCCTCA AGACAGCACA GGGGCAGTGG CCAGCCTTGG GGTAGCCCCT
    CCTCAGCCAC CTTCTCAGAC CTTCCCCCCA CCCTCCCTCC CCTCAAAAAG CCTTGCAATG
    CAGCAGAAGC CCACGGCTTA TGTCCGGCCC ATGGACGGTC AAGACCAGGC TCCTAGCGAG
    TCTCCTGAAC TGAAACCCCT GCCGGAGGAG TACCGGCAGC AGACCTTTGA GAAATCAGAC
    TTAAAAGTGC CTGCCAAAGC CAAGCTCACG AAGCTGAAAA TGCCTTCTCA GTCAGTCGAG
    CAGACCTACT CCAATGAAGT CCATTGTGTT GAAGAGATTC TGAAGGAAAT GACCCACTCA
    TGGCCTCCTC CTTTGACAGC AATACACACG CCTAGTACAG CTGAGCCCTC CAAATTTCCT
    TTTCCCACAA AGGATTCTCA GCACATCAGT TCTGCGGTCC CAAACCAAAA ACAATATGAT
    ACATCTTCAA AAACTCACAG TAATTCTCAG CAGGGAACAT CCATGCTCAA AGATGACCTT
    CAGCTCAGTG ACAGTGAGGA CAGTGACAGC GATCAAACCT CAGAGAAGCC TCCTTTCGCA
    CCTGCACCTC CAAGCGCTCC ACCGGCGCTG CCAGCAGCCG CGGCCTCAGC ACATTCCAGC
    AGCGCCGAAT CGGAGAGCAC CAGCGACTCC GACAGCTCCT CGGACTCGGA GAGCGAGAGC
    AGCTCCAGTG ACAGCGAGGA GAACGAACCC CGAGAAGCCC CGGCTCCCGA GCCCGAGCCT
    CCGACAACCA ACAAATGGCA GCTGGACAAC TGGCTGACCA AAGTCAGCCA GCCCGCGGTG
    CCCACCGAGG CCCCGGGTAG CACGGAGCCC CCGGCCCGAC CCCCGGACAC TAAGGGCAAG
    GGCGGCGACA GCGCCACCGC GGGTCACGAG CGCCCAGAGT CCAAAGAGCC TCCCCCCAAA
    GGCTCCAGCA AAGCCCCCCG GGTCCCATCC GAAGGCCCCC ACGCGGGCAA GAGGAGCTGT
    CAGAAGTCCC CTACCCAGCA GGAGCCCCCC CAGCGGCAAA CCGTGGGAAG CAAACAGCCG
    AAAAAGCCCA TCAAGGCTTC CGCCCCCGGG CACGCGGATG CGCCTGCAGA TGCGCGTGCC
    TGCCTGCAGG TGGAGAGCGA GCCCGGACTT CCTCCCCCGG CCTCCAGGGA CCAGCCCTCC
    AAAGACAAGC CCAAGGTGAA GACGAAAGGG CGGCCCCGCG CCGCAGACAG CCGGGAGCCC
    AAGCCGGCGG TGCCCACCCC CAGCGACCGG AAGAAGCACC GCAGCGGCCC CCCGAAGGCG
    CCCCCGAAGG ACGCGGCGGC AGAGGACCGG AGCCCCGAAC ACTTTGCGCT CGTTCCCCTG
    ACCCAGAGCC AGGGCCCTGT CCGCGGTGGC AGCGGCGGCG CCGGCGCCAG GACTAGTGGC
    TGCAGGCTGG CTGTGGTGGT CCAGGAGGAC CGCCGCAAAG ACAAACCCCC GGTGCCTTCG
    AGAGACACCA AGCTGCTCTC CCCGCTCAGG GACGCTCCGC CGCCACAAAG CTTGGTGGTG
    AAGATCACAC TCGACCTCCT GTCTCGGATA CCCCAGCCCC CAGGGAAGGG CAGCCGCCCC
    AAGAAACCAG AAGACAAACA GCCACCAGCA GGGAAGAAGC CAGATCCTGA GAAGAGAAGC
    TCAGAAAACG CCAGCAAGTT AGCCAAAAAG AGAAAGGGTG AAGCAGAGAA AGACCACGAT
    AGCAAGAAAG TCAGGCTGGA GAAGGAAGTC AAGTCACAGC CGTTGTCTTC CTCTTCCCAC
    AAGGAATCTT CCAAATCAAA GATGTCCAGG CCCTCCTCTG AGCCCTCAAA GAAGGAAATG
    CTTCCCCCTT CGCTCGTGTC ATCCTCATCC TCCCAGAAGC CAGCCAAGCC TGCACAGAAG
    AGGCCACGGC AGGAAGACCC CGGCTGCCAG GACCCCCAAA AAAGTGCCGG TAGTGCCAAG
    GGCAGCCACA GAGACCCTTC TGCCTCCAAA CACAGAAAAG CAGAGGGAAA GGTCTCTGGA
    AGCTCCACAG AGCACAAAGG ATCTTCTGGA GATACTGCAA ACCGTTTTCC AGTGCCTTCT
    TTGCCAAATG GTAACTCCAA ACCAGGGAAG CCTCACGTGA AGTTTGACAA ACAACAAGCC
    GATTTTCACC TGAAGGAAGC CAAAAGGTTG AAGGACAAAG CAGAATTAGT GACGGACAAG
    GTAGGGAAGG CCTTTAAATA CCTGGAGGCT GCCTTGTCCT TCATTGAGTG CGGCGTCGCC
    CTGGAGTCGG AGAGCCCCGC GTCCAAGTCG GCGTACTCCA TCTACTCGGA GACCGTGGAC
    CTCATTAAGT TCATACTGTC ATTAAAATCC TTCTCAGATG CCACAACGCC AACACAGGAG
    AAGATATTTG CTGTTCTATG CATGCGTTGC CAGTCCATTT TGAACATGGC AATGTTTCGT
    TGTAAAAAAG ATATGGCGAT AAAGTATTCC CGCACGCTTA ACGAACACTT CTTCAAGACT
    TCTTCCAAGG TTGCCCAGGC ACCTTCTCCA TGCATCGCAA GAAGCACAGG CACACCGTCC
    CCCCTCTCTC CGATCCCTTC TCCGGCCAGC TCCATAGGGT CCCAGTCAAG CGCTGGCAGC
    GTGGGGAGCG GGGCGCTGCC CGCCACCATC AGCACCCCCG TCACCATCCA GAACATGACA
    TCCTCCTACG TCAGCATCAC TTCCCACGTC CTTACCGCCT TTGACCTGTG GGAACAGGCT
    GAGGTCCTTA CCAGGAAGAA CAAAGAATTC TTTGCTCAGC TCAGCACAAG TGTGTGCGCG
    CTGGCCCTCA ACAGCAGTTT GATGGATCTG GTGCATTATA CAAGACAGGG TTTCCAGCGG
    CTAAAGCAAG TAACCAAGAC ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235456
    Clone ID Related Accession (Same CDS sequence) XM_024993466.1 , XM_010806300.3
    Accession Version XM_010806300.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3687bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806300.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CCGAAAGAGT CAACAGCAGC AGCAACAGCT TGTACAATGA AGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CTCACCAAGA TAAAGAGGCA
    TTTTCTGAAA AGATCCCCCT TTTTGGAGAG CCATACAAGA CAGAGAAAGG TGATGAGCTA
    TCCAGTCGGA TACAGAACAT GCTGGGAAAC TATGAAGAGA TGAAGGAGTT CCTTAGTAGC
    AAGTCCCACC CCCAGCGCTT GGATGCTTCT GAAAATAGGC TGGGGAAGCC GAGATACCCT
    TTATTTCCTG AGAAGGGGAG CAGCCTTCCA CCCCACTCCT TCCACACCAG TGTCCACCAC
    CAGCCCGTTA ACACTCCTGC CTGTGGGCCA CATCCTGCTG GTAACATCGG CCACCACCCA
    AAGATGGCGC AGCCAAGAAT GGAACCAATG CCAAGTCTCC ACGCCAAAAG CTATGGCCCA
    CCGGACAGCC AGCACCTGAC CCAAGAGCGC CTCGGTCAGG AGGGGTACGG CTCTAGTCAT
    CACAAAAAGG GTGACCGCAG GGCTGATGGA GACCACTGTG CTTTGACGAC AGACTCTGCT
    CCGGAGAGGG AACTTTCACC TCTGTTCTCT TTGCCTTCCC CAGTCCCCCC TTTGTCACCT
    GTACATTCCA ACCAGCAGAC TGCTCCCAGG ACGCAAGGAA GCGGCAAAGT TCCCAGCAGT
    AGCAGTAGCA ATAAAGGCTA CTGCCTGGCC AAGTCTCCCA AGGACCTAGC GGTAAAGGTG
    CACGATAAAG AGACCCCTCA AGACAGCACA GGGGCAGTGG CCAGCCTTGG GGTAGCCCCT
    CCTCAGCCAC CTTCTCAGAC CTTCCCCCCA CCCTCCCTCC CCTCAAAAAG CCTTGCAATG
    CAGCAGAAGC CCACGGCTTA TGTCCGGCCC ATGGACGGTC AAGACCAGGC TCCTAGCGAG
    TCTCCTGAAC TGAAACCCCT GCCGGAGGAG TACCGGCAGC AGACCTTTGA GAAATCAGAC
    TTAAAAGTGC CTGCCAAAGC CAAGCTCACG AAGCTGAAAA TGCCTTCTCA GTCAGTCGAG
    CAGACCTACT CCAATGAAGT CCATTGTGTT GAAGAGATTC TGAAGGAAAT GACCCACTCA
    TGGCCTCCTC CTTTGACAGC AATACACACG CCTAGTACAG CTGAGCCCTC CAAATTTCCT
    TTTCCCACAA AGGATTCTCA GCACATCAGT TCTGCGGTCC CAAACCAAAA ACAATATGAT
    ACATCTTCAA AAACTCACAG TAATTCTCAG CAGGGAACAT CCATGCTCAA AGATGACCTT
    CAGCTCAGTG ACAGTGAGGA CAGTGACAGC GATCAAACCT CAGAGAAGCC TCCTTTCGCA
    CCTGCACCTC CAAGCGCTCC ACCGGCGCTG CCAGCAGCCG CGGCCTCAGC ACATTCCAGC
    AGCGCCGAAT CGGAGAGCAC CAGCGACTCC GACAGCTCCT CGGACTCGGA GAGCGAGAGC
    AGCTCCAGTG ACAGCGAGGA GAACGAACCC CGAGAAGCCC CGGCTCCCGA GCCCGAGCCT
    CCGACAACCA ACAAATGGCA GCTGGACAAC TGGCTGACCA AAGTCAGCCA GCCCGCGGTG
    CCCACCGAGG CCCCGGGTAG CACGGAGCCC CCGGCCCGAC CCCCGGACAC TAAGGGCAAG
    GGCGGCGACA GCGCCACCGC GGGTCACGAG CGCCCAGAGT CCAAAGAGCC TCCCCCCAAA
    GGCTCCAGCA AAGCCCCCCG GGTCCCATCC GAAGGCCCCC ACGCGGGCAA GAGGAGCTGT
    CAGAAGTCCC CTACCCAGCA GGAGCCCCCC CAGCGGCAAA CCGTGGGAAG CAAACAGCCG
    AAAAAGCCCA TCAAGGCTTC CGCCCCCGGG CACGCGGATG CGCCTGCAGA TGCGCGTGCC
    TGCCTGCAGG TGGAGAGCGA GCCCGGACTT CCTCCCCCGG CCTCCAGGGA CCAGCCCTCC
    AAAGACAAGC CCAAGGTGAA GACGAAAGGG CGGCCCCGCG CCGCAGACAG CCGGGAGCCC
    AAGCCGGCGG TGCCCACCCC CAGCGACCGG AAGAAGCACC GCAGCGGCCC CCCGAAGGCG
    CCCCCGAAGG ACGCGGCGGC AGAGGACCGG AGCCCCGAAC ACTTTGCGCT CGTTCCCCTG
    ACCCAGAGCC AGGGCCCTGT CCGCGGTGGC AGCGGCGGCG CCGGCGCCAG GACTAGTGGC
    TGCAGGCTGG CTGTGGTGGT CCAGGAGGAC CGCCGCAAAG ACAAACCCCC GGTGCCTTCG
    AGAGACACCA AGCTGCTCTC CCCGCTCAGG GACGCTCCGC CGCCACAAAG CTTGGTGGTG
    AAGATCACAC TCGACCTCCT GTCTCGGATA CCCCAGCCCC CAGGGAAGGG CAGCCGCCCC
    AAGAAACCAG AAGACAAACA GCCACCAGCA GGGAAGAAGC CAGATCCTGA GAAGAGAAGC
    TCAGAAAACG CCAGCAAGTT AGCCAAAAAG AGAAAGGGTG AAGCAGAGAA AGACCACGAT
    AGCAAGAAAG TCAGGCTGGA GAAGGAAGTC AAGTCACAGC CGTTGTCTTC CTCTTCCCAC
    AAGGAATCTT CCAAATCAAA GATGTCCAGG CCCTCCTCTG AGCCCTCAAA GAAGGAAATG
    CTTCCCCCTT CGCTCGTGTC ATCCTCATCC TCCCAGAAGC CAGCCAAGCC TGCACAGAAG
    AGGCCACGGC AGGAAGACCC CGGCTGCCAG GACCCCCAAA AAAGTGCCGG TAGTGCCAAG
    GGCAGCCACA GAGACCCTTC TGCCTCCAAA CACAGAAAAG CAGAGGGAAA GGTCTCTGGA
    AGCTCCACAG AGCACAAAGG ATCTTCTGGA GATACTGCAA ACCGTTTTCC AGTGCCTTCT
    TTGCCAAATG GTAACTCCAA ACCAGGGAAG CCTCACGTGA AGTTTGACAA ACAACAAGCC
    GATTTTCACC TGAAGGAAGC CAAAAGGTTG AAGGACAAAG CAGAATTAGT GACGGACAAG
    GTAGGGAAGG CCTTTAAATA CCTGGAGGCT GCCTTGTCCT TCATTGAGTG CGGCGTCGCC
    CTGGAGTCGG AGAGCCCCGC GTCCAAGTCG GCGTACTCCA TCTACTCGGA GACCGTGGAC
    CTCATTAAGT TCATACTGTC ATTAAAATCC TTCTCAGATG CCACAACGCC AACACAGGAG
    AAGATATTTG CTGTTCTATG CATGCGTTGC CAGTCCATTT TGAACATGGC AATGTTTCGT
    TGTAAAAAAG ATATGGCGAT AAAGTATTCC CGCACGCTTA ACGAACACTT CTTCAAGACT
    TCTTCCAAGG TTGCCCAGGC ACCTTCTCCA TGCATCGCAA GAAGCACAGG CACACCGTCC
    CCCCTCTCTC CGATCCCTTC TCCGGCCAGC TCCATAGGGT CCCAGTCAAG CGCTGGCAGC
    GTGGGGAGCG GGGCGCTGCC CGCCACCATC AGCACCCCCG TCACCATCCA GAACATGACA
    TCCTCCTACG TCAGCATCAC TTCCCACGTC CTTACCGCCT TTGACCTGTG GGAACAGGCT
    GAGGTCCTTA CCAGGAAGAA CAAAGAATTC TTTGCTCAGC TCAGCACAAG TGTGTGCGCG
    CTGGCCCTCA ACAGCAGTTT GATGGATCTG GTGCATTATA CAAGACAGGG TTTCCAGCGG
    CTAAAGCAAG TAACCAAGAC ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804602.2
    CDS316..4002
    Translation

    Target ORF information:

    RefSeq Version XM_010806300.3
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806300.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CCGAAAGAGT CAACAGCAGC AGCAACAGCT TGTACAATGA AGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CTCACCAAGA TAAAGAGGCA
    TTTTCTGAAA AGATCCCCCT TTTTGGAGAG CCATACAAGA CAGAGAAAGG TGATGAGCTA
    TCCAGTCGGA TACAGAACAT GCTGGGAAAC TATGAAGAGA TGAAGGAGTT CCTTAGTAGC
    AAGTCCCACC CCCAGCGCTT GGATGCTTCT GAAAATAGGC TGGGGAAGCC GAGATACCCT
    TTATTTCCTG AGAAGGGGAG CAGCCTTCCA CCCCACTCCT TCCACACCAG TGTCCACCAC
    CAGCCCGTTA ACACTCCTGC CTGTGGGCCA CATCCTGCTG GTAACATCGG CCACCACCCA
    AAGATGGCGC AGCCAAGAAT GGAACCAATG CCAAGTCTCC ACGCCAAAAG CTATGGCCCA
    CCGGACAGCC AGCACCTGAC CCAAGAGCGC CTCGGTCAGG AGGGGTACGG CTCTAGTCAT
    CACAAAAAGG GTGACCGCAG GGCTGATGGA GACCACTGTG CTTTGACGAC AGACTCTGCT
    CCGGAGAGGG AACTTTCACC TCTGTTCTCT TTGCCTTCCC CAGTCCCCCC TTTGTCACCT
    GTACATTCCA ACCAGCAGAC TGCTCCCAGG ACGCAAGGAA GCGGCAAAGT TCCCAGCAGT
    AGCAGTAGCA ATAAAGGCTA CTGCCTGGCC AAGTCTCCCA AGGACCTAGC GGTAAAGGTG
    CACGATAAAG AGACCCCTCA AGACAGCACA GGGGCAGTGG CCAGCCTTGG GGTAGCCCCT
    CCTCAGCCAC CTTCTCAGAC CTTCCCCCCA CCCTCCCTCC CCTCAAAAAG CCTTGCAATG
    CAGCAGAAGC CCACGGCTTA TGTCCGGCCC ATGGACGGTC AAGACCAGGC TCCTAGCGAG
    TCTCCTGAAC TGAAACCCCT GCCGGAGGAG TACCGGCAGC AGACCTTTGA GAAATCAGAC
    TTAAAAGTGC CTGCCAAAGC CAAGCTCACG AAGCTGAAAA TGCCTTCTCA GTCAGTCGAG
    CAGACCTACT CCAATGAAGT CCATTGTGTT GAAGAGATTC TGAAGGAAAT GACCCACTCA
    TGGCCTCCTC CTTTGACAGC AATACACACG CCTAGTACAG CTGAGCCCTC CAAATTTCCT
    TTTCCCACAA AGGATTCTCA GCACATCAGT TCTGCGGTCC CAAACCAAAA ACAATATGAT
    ACATCTTCAA AAACTCACAG TAATTCTCAG CAGGGAACAT CCATGCTCAA AGATGACCTT
    CAGCTCAGTG ACAGTGAGGA CAGTGACAGC GATCAAACCT CAGAGAAGCC TCCTTTCGCA
    CCTGCACCTC CAAGCGCTCC ACCGGCGCTG CCAGCAGCCG CGGCCTCAGC ACATTCCAGC
    AGCGCCGAAT CGGAGAGCAC CAGCGACTCC GACAGCTCCT CGGACTCGGA GAGCGAGAGC
    AGCTCCAGTG ACAGCGAGGA GAACGAACCC CGAGAAGCCC CGGCTCCCGA GCCCGAGCCT
    CCGACAACCA ACAAATGGCA GCTGGACAAC TGGCTGACCA AAGTCAGCCA GCCCGCGGTG
    CCCACCGAGG CCCCGGGTAG CACGGAGCCC CCGGCCCGAC CCCCGGACAC TAAGGGCAAG
    GGCGGCGACA GCGCCACCGC GGGTCACGAG CGCCCAGAGT CCAAAGAGCC TCCCCCCAAA
    GGCTCCAGCA AAGCCCCCCG GGTCCCATCC GAAGGCCCCC ACGCGGGCAA GAGGAGCTGT
    CAGAAGTCCC CTACCCAGCA GGAGCCCCCC CAGCGGCAAA CCGTGGGAAG CAAACAGCCG
    AAAAAGCCCA TCAAGGCTTC CGCCCCCGGG CACGCGGATG CGCCTGCAGA TGCGCGTGCC
    TGCCTGCAGG TGGAGAGCGA GCCCGGACTT CCTCCCCCGG CCTCCAGGGA CCAGCCCTCC
    AAAGACAAGC CCAAGGTGAA GACGAAAGGG CGGCCCCGCG CCGCAGACAG CCGGGAGCCC
    AAGCCGGCGG TGCCCACCCC CAGCGACCGG AAGAAGCACC GCAGCGGCCC CCCGAAGGCG
    CCCCCGAAGG ACGCGGCGGC AGAGGACCGG AGCCCCGAAC ACTTTGCGCT CGTTCCCCTG
    ACCCAGAGCC AGGGCCCTGT CCGCGGTGGC AGCGGCGGCG CCGGCGCCAG GACTAGTGGC
    TGCAGGCTGG CTGTGGTGGT CCAGGAGGAC CGCCGCAAAG ACAAACCCCC GGTGCCTTCG
    AGAGACACCA AGCTGCTCTC CCCGCTCAGG GACGCTCCGC CGCCACAAAG CTTGGTGGTG
    AAGATCACAC TCGACCTCCT GTCTCGGATA CCCCAGCCCC CAGGGAAGGG CAGCCGCCCC
    AAGAAACCAG AAGACAAACA GCCACCAGCA GGGAAGAAGC CAGATCCTGA GAAGAGAAGC
    TCAGAAAACG CCAGCAAGTT AGCCAAAAAG AGAAAGGGTG AAGCAGAGAA AGACCACGAT
    AGCAAGAAAG TCAGGCTGGA GAAGGAAGTC AAGTCACAGC CGTTGTCTTC CTCTTCCCAC
    AAGGAATCTT CCAAATCAAA GATGTCCAGG CCCTCCTCTG AGCCCTCAAA GAAGGAAATG
    CTTCCCCCTT CGCTCGTGTC ATCCTCATCC TCCCAGAAGC CAGCCAAGCC TGCACAGAAG
    AGGCCACGGC AGGAAGACCC CGGCTGCCAG GACCCCCAAA AAAGTGCCGG TAGTGCCAAG
    GGCAGCCACA GAGACCCTTC TGCCTCCAAA CACAGAAAAG CAGAGGGAAA GGTCTCTGGA
    AGCTCCACAG AGCACAAAGG ATCTTCTGGA GATACTGCAA ACCGTTTTCC AGTGCCTTCT
    TTGCCAAATG GTAACTCCAA ACCAGGGAAG CCTCACGTGA AGTTTGACAA ACAACAAGCC
    GATTTTCACC TGAAGGAAGC CAAAAGGTTG AAGGACAAAG CAGAATTAGT GACGGACAAG
    GTAGGGAAGG CCTTTAAATA CCTGGAGGCT GCCTTGTCCT TCATTGAGTG CGGCGTCGCC
    CTGGAGTCGG AGAGCCCCGC GTCCAAGTCG GCGTACTCCA TCTACTCGGA GACCGTGGAC
    CTCATTAAGT TCATACTGTC ATTAAAATCC TTCTCAGATG CCACAACGCC AACACAGGAG
    AAGATATTTG CTGTTCTATG CATGCGTTGC CAGTCCATTT TGAACATGGC AATGTTTCGT
    TGTAAAAAAG ATATGGCGAT AAAGTATTCC CGCACGCTTA ACGAACACTT CTTCAAGACT
    TCTTCCAAGG TTGCCCAGGC ACCTTCTCCA TGCATCGCAA GAAGCACAGG CACACCGTCC
    CCCCTCTCTC CGATCCCTTC TCCGGCCAGC TCCATAGGGT CCCAGTCAAG CGCTGGCAGC
    GTGGGGAGCG GGGCGCTGCC CGCCACCATC AGCACCCCCG TCACCATCCA GAACATGACA
    TCCTCCTACG TCAGCATCAC TTCCCACGTC CTTACCGCCT TTGACCTGTG GGAACAGGCT
    GAGGTCCTTA CCAGGAAGAA CAAAGAATTC TTTGCTCAGC TCAGCACAAG TGTGTGCGCG
    CTGGCCCTCA ACAGCAGTTT GATGGATCTG GTGCATTATA CAAGACAGGG TTTCCAGCGG
    CTAAAGCAAG TAACCAAGAC ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235457
    Clone ID Related Accession (Same CDS sequence) XM_010806302.3
    Accession Version XM_010806302.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806302.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCCC AGTCAAGCAG CTTGTACAAT GAAGACAGAA ACCTGCTTCG AATTAGAGAG 
    AAGGAAAGAC GCAACCAGGA AGCTCACCAA GATAAAGAGG CATTTTCTGA AAAGATCCCC
    CTTTTTGGAG AGCCATACAA GACAGAGAAA GGTGATGAGC TATCCAGTCG GATACAGAAC
    ATGCTGGGAA ACTATGAAGA GATGAAGGAG TTCCTTAGTA GCAAGTCCCA CCCCCAGCGC
    TTGGATGCTT CTGAAAATAG GCTGGGGAAG CCGAGATACC CTTTATTTCC TGAGAAGGGG
    AGCAGCCTTC CACCCCACTC CTTCCACACC AGTGTCCACC ACCAGCCCGT TAACACTCCT
    GCCTGTGGGC CACATCCTGC TGGTAACATC GGCCACCACC CAAAGATGGC GCAGCCAAGA
    ATGGAACCAA TGCCAAGTCT CCACGCCAAA AGCTATGGCC CACCGGACAG CCAGCACCTG
    ACCCAAGAGC GCCTCGGTCA GGAGGGGTAC GGCTCTAGTC ATCACAAAAA GGGTGACCGC
    AGGGCTGATG GAGACCACTG TGCTTTGACG ACAGACTCTG CTCCGGAGAG GGAACTTTCA
    CCTCTGTTCT CTTTGCCTTC CCCAGTCCCC CCTTTGTCAC CTGTACATTC CAACCAGCAG
    ACTGCTCCCA GGACGCAAGG AAGCGGCAAA GTTCCCAGCA GTAGCAGTAG CAATAAAGGC
    TACTGCCTGG CCAAGTCTCC CAAGGACCTA GCGGTAAAGG TGCACGATAA AGAGACCCCT
    CAAGACAGCA CAGGGGCAGT GGCCAGCCTT GGGGTAGCCC CTCCTCAGCC ACCTTCTCAG
    ACCTTCCCCC CACCCTCCCT CCCCTCAAAA AGCCTTGCAA TGCAGCAGAA GCCCACGGCT
    TATGTCCGGC CCATGGACGG TCAAGACCAG GCTCCTAGCG AGTCTCCTGA ACTGAAACCC
    CTGCCGGAGG AGTACCGGCA GCAGACCTTT GAGAAATCAG ACTTAAAAGT GCCTGCCAAA
    GCCAAGCTCA CGAAGCTGAA AATGCCTTCT CAGTCAGTCG AGCAGACCTA CTCCAATGAA
    GTCCATTGTG TTGAAGAGAT TCTGAAGGAA ATGACCCACT CATGGCCTCC TCCTTTGACA
    GCAATACACA CGCCTAGTAC AGCTGAGCCC TCCAAATTTC CTTTTCCCAC AAAGGATTCT
    CAGCACATCA GTTCTGCGGT CCCAAACCAA AAACAATATG ATACATCTTC AAAAACTCAC
    AGTAATTCTC AGCAGGGAAC ATCCATGCTC AAAGATGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GCGATCAAAC CTCAGAGAAG CCTCCTTTCG CACCTGCACC TCCAAGCGCT
    CCACCGGCGC TGCCAGCAGC CGCGGCCTCA GCACATTCCA GCAGCGCCGA ATCGGAGAGC
    ACCAGCGACT CCGACAGCTC CTCGGACTCG GAGAGCGAGA GCAGCTCCAG TGACAGCGAG
    GAGAACGAAC CCCGAGAAGC CCCGGCTCCC GAGCCCGAGC CTCCGACAAC CAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCCGCGG TGCCCACCGA GGCCCCGGGT
    AGCACGGAGC CCCCGGCCCG ACCCCCGGAC ACTAAGGGCA AGGGCGGCGA CAGCGCCACC
    GCGGGTCACG AGCGCCCAGA GTCCAAAGAG CCTCCCCCCA AAGGCTCCAG CAAAGCCCCC
    CGGGTCCCAT CCGAAGGCCC CCACGCGGGC AAGAGGAGCT GTCAGAAGTC CCCTACCCAG
    CAGGAGCCCC CCCAGCGGCA AACCGTGGGA AGCAAACAGC CGAAAAAGCC CATCAAGGCT
    TCCGCCCCCG GGCACGCGGA TGCGCCTGCA GATGCGCGTG CCTGCCTGCA GGTGGAGAGC
    GAGCCCGGAC TTCCTCCCCC GGCCTCCAGG GACCAGCCCT CCAAAGACAA GCCCAAGGTG
    AAGACGAAAG GGCGGCCCCG CGCCGCAGAC AGCCGGGAGC CCAAGCCGGC GGTGCCCACC
    CCCAGCGACC GGAAGAAGCA CCGCAGCGGC CCCCCGAAGG CGCCCCCGAA GGACGCGGCG
    GCAGAGGACC GGAGCCCCGA ACACTTTGCG CTCGTTCCCC TGACCCAGAG CCAGGGCCCT
    GTCCGCGGTG GCAGCGGCGG CGCCGGCGCC AGGACTAGTG GCTGCAGGCT GGCTGTGGTG
    GTCCAGGAGG ACCGCCGCAA AGACAAACCC CCGGTGCCTT CGAGAGACAC CAAGCTGCTC
    TCCCCGCTCA GGGACGCTCC GCCGCCACAA AGCTTGGTGG TGAAGATCAC ACTCGACCTC
    CTGTCTCGGA TACCCCAGCC CCCAGGGAAG GGCAGCCGCC CCAAGAAACC AGAAGACAAA
    CAGCCACCAG CAGGGAAGAA GCCAGATCCT GAGAAGAGAA GCTCAGAAAA CGCCAGCAAG
    TTAGCCAAAA AGAGAAAGGG TGAAGCAGAG AAAGACCACG ATAGCAAGAA AGTCAGGCTG
    GAGAAGGAAG TCAAGTCACA GCCGTTGTCT TCCTCTTCCC ACAAGGAATC TTCCAAATCA
    AAGATGTCCA GGCCCTCCTC TGAGCCCTCA AAGAAGGAAA TGCTTCCCCC TTCGCTCGTG
    TCATCCTCAT CCTCCCAGAA GCCAGCCAAG CCTGCACAGA AGAGGCCACG GCAGGAAGAC
    CCCGGCTGCC AGGACCCCCA AAAAAGTGCC GGTAGTGCCA AGGGCAGCCA CAGAGACCCT
    TCTGCCTCCA AACACAGAAA AGCAGAGGGA AAGGTCTCTG GAAGCTCCAC AGAGCACAAA
    GGATCTTCTG GAGATACTGC AAACCGTTTT CCAGTGCCTT CTTTGCCAAA TGGTAACTCC
    AAACCAGGGA AGCCTCACGT GAAGTTTGAC AAACAACAAG CCGATTTTCA CCTGAAGGAA
    GCCAAAAGGT TGAAGGACAA AGCAGAATTA GTGACGGACA AGGTAGGGAA GGCCTTTAAA
    TACCTGGAGG CTGCCTTGTC CTTCATTGAG TGCGGCGTCG CCCTGGAGTC GGAGAGCCCC
    GCGTCCAAGT CGGCGTACTC CATCTACTCG GAGACCGTGG ACCTCATTAA GTTCATACTG
    TCATTAAAAT CCTTCTCAGA TGCCACAACG CCAACACAGG AGAAGATATT TGCTGTTCTA
    TGCATGCGTT GCCAGTCCAT TTTGAACATG GCAATGTTTC GTTGTAAAAA AGATATGGCG
    ATAAAGTATT CCCGCACGCT TAACGAACAC TTCTTCAAGA CTTCTTCCAA GGTTGCCCAG
    GCACCTTCTC CATGCATCGC AAGAAGCACA GGCACACCGT CCCCCCTCTC TCCGATCCCT
    TCTCCGGCCA GCTCCATAGG GTCCCAGTCA AGCGCTGGCA GCGTGGGGAG CGGGGCGCTG
    CCCGCCACCA TCAGCACCCC CGTCACCATC CAGAACATGA CATCCTCCTA CGTCAGCATC
    ACTTCCCACG TCCTTACCGC CTTTGACCTG TGGGAACAGG CTGAGGTCCT TACCAGGAAG
    AACAAAGAAT TCTTTGCTCA GCTCAGCACA AGTGTGTGCG CGCTGGCCCT CAACAGCAGT
    TTGATGGATC TGGTGCATTA TACAAGACAG GGTTTCCAGC GGCTAAAGCA AGTAACCAAG
    ACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804604.2
    CDS300..3968
    Translation

    Target ORF information:

    RefSeq Version XM_010806302.3
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806302.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCCC AGTCAAGCAG CTTGTACAAT GAAGACAGAA ACCTGCTTCG AATTAGAGAG 
    AAGGAAAGAC GCAACCAGGA AGCTCACCAA GATAAAGAGG CATTTTCTGA AAAGATCCCC
    CTTTTTGGAG AGCCATACAA GACAGAGAAA GGTGATGAGC TATCCAGTCG GATACAGAAC
    ATGCTGGGAA ACTATGAAGA GATGAAGGAG TTCCTTAGTA GCAAGTCCCA CCCCCAGCGC
    TTGGATGCTT CTGAAAATAG GCTGGGGAAG CCGAGATACC CTTTATTTCC TGAGAAGGGG
    AGCAGCCTTC CACCCCACTC CTTCCACACC AGTGTCCACC ACCAGCCCGT TAACACTCCT
    GCCTGTGGGC CACATCCTGC TGGTAACATC GGCCACCACC CAAAGATGGC GCAGCCAAGA
    ATGGAACCAA TGCCAAGTCT CCACGCCAAA AGCTATGGCC CACCGGACAG CCAGCACCTG
    ACCCAAGAGC GCCTCGGTCA GGAGGGGTAC GGCTCTAGTC ATCACAAAAA GGGTGACCGC
    AGGGCTGATG GAGACCACTG TGCTTTGACG ACAGACTCTG CTCCGGAGAG GGAACTTTCA
    CCTCTGTTCT CTTTGCCTTC CCCAGTCCCC CCTTTGTCAC CTGTACATTC CAACCAGCAG
    ACTGCTCCCA GGACGCAAGG AAGCGGCAAA GTTCCCAGCA GTAGCAGTAG CAATAAAGGC
    TACTGCCTGG CCAAGTCTCC CAAGGACCTA GCGGTAAAGG TGCACGATAA AGAGACCCCT
    CAAGACAGCA CAGGGGCAGT GGCCAGCCTT GGGGTAGCCC CTCCTCAGCC ACCTTCTCAG
    ACCTTCCCCC CACCCTCCCT CCCCTCAAAA AGCCTTGCAA TGCAGCAGAA GCCCACGGCT
    TATGTCCGGC CCATGGACGG TCAAGACCAG GCTCCTAGCG AGTCTCCTGA ACTGAAACCC
    CTGCCGGAGG AGTACCGGCA GCAGACCTTT GAGAAATCAG ACTTAAAAGT GCCTGCCAAA
    GCCAAGCTCA CGAAGCTGAA AATGCCTTCT CAGTCAGTCG AGCAGACCTA CTCCAATGAA
    GTCCATTGTG TTGAAGAGAT TCTGAAGGAA ATGACCCACT CATGGCCTCC TCCTTTGACA
    GCAATACACA CGCCTAGTAC AGCTGAGCCC TCCAAATTTC CTTTTCCCAC AAAGGATTCT
    CAGCACATCA GTTCTGCGGT CCCAAACCAA AAACAATATG ATACATCTTC AAAAACTCAC
    AGTAATTCTC AGCAGGGAAC ATCCATGCTC AAAGATGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GCGATCAAAC CTCAGAGAAG CCTCCTTTCG CACCTGCACC TCCAAGCGCT
    CCACCGGCGC TGCCAGCAGC CGCGGCCTCA GCACATTCCA GCAGCGCCGA ATCGGAGAGC
    ACCAGCGACT CCGACAGCTC CTCGGACTCG GAGAGCGAGA GCAGCTCCAG TGACAGCGAG
    GAGAACGAAC CCCGAGAAGC CCCGGCTCCC GAGCCCGAGC CTCCGACAAC CAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCCGCGG TGCCCACCGA GGCCCCGGGT
    AGCACGGAGC CCCCGGCCCG ACCCCCGGAC ACTAAGGGCA AGGGCGGCGA CAGCGCCACC
    GCGGGTCACG AGCGCCCAGA GTCCAAAGAG CCTCCCCCCA AAGGCTCCAG CAAAGCCCCC
    CGGGTCCCAT CCGAAGGCCC CCACGCGGGC AAGAGGAGCT GTCAGAAGTC CCCTACCCAG
    CAGGAGCCCC CCCAGCGGCA AACCGTGGGA AGCAAACAGC CGAAAAAGCC CATCAAGGCT
    TCCGCCCCCG GGCACGCGGA TGCGCCTGCA GATGCGCGTG CCTGCCTGCA GGTGGAGAGC
    GAGCCCGGAC TTCCTCCCCC GGCCTCCAGG GACCAGCCCT CCAAAGACAA GCCCAAGGTG
    AAGACGAAAG GGCGGCCCCG CGCCGCAGAC AGCCGGGAGC CCAAGCCGGC GGTGCCCACC
    CCCAGCGACC GGAAGAAGCA CCGCAGCGGC CCCCCGAAGG CGCCCCCGAA GGACGCGGCG
    GCAGAGGACC GGAGCCCCGA ACACTTTGCG CTCGTTCCCC TGACCCAGAG CCAGGGCCCT
    GTCCGCGGTG GCAGCGGCGG CGCCGGCGCC AGGACTAGTG GCTGCAGGCT GGCTGTGGTG
    GTCCAGGAGG ACCGCCGCAA AGACAAACCC CCGGTGCCTT CGAGAGACAC CAAGCTGCTC
    TCCCCGCTCA GGGACGCTCC GCCGCCACAA AGCTTGGTGG TGAAGATCAC ACTCGACCTC
    CTGTCTCGGA TACCCCAGCC CCCAGGGAAG GGCAGCCGCC CCAAGAAACC AGAAGACAAA
    CAGCCACCAG CAGGGAAGAA GCCAGATCCT GAGAAGAGAA GCTCAGAAAA CGCCAGCAAG
    TTAGCCAAAA AGAGAAAGGG TGAAGCAGAG AAAGACCACG ATAGCAAGAA AGTCAGGCTG
    GAGAAGGAAG TCAAGTCACA GCCGTTGTCT TCCTCTTCCC ACAAGGAATC TTCCAAATCA
    AAGATGTCCA GGCCCTCCTC TGAGCCCTCA AAGAAGGAAA TGCTTCCCCC TTCGCTCGTG
    TCATCCTCAT CCTCCCAGAA GCCAGCCAAG CCTGCACAGA AGAGGCCACG GCAGGAAGAC
    CCCGGCTGCC AGGACCCCCA AAAAAGTGCC GGTAGTGCCA AGGGCAGCCA CAGAGACCCT
    TCTGCCTCCA AACACAGAAA AGCAGAGGGA AAGGTCTCTG GAAGCTCCAC AGAGCACAAA
    GGATCTTCTG GAGATACTGC AAACCGTTTT CCAGTGCCTT CTTTGCCAAA TGGTAACTCC
    AAACCAGGGA AGCCTCACGT GAAGTTTGAC AAACAACAAG CCGATTTTCA CCTGAAGGAA
    GCCAAAAGGT TGAAGGACAA AGCAGAATTA GTGACGGACA AGGTAGGGAA GGCCTTTAAA
    TACCTGGAGG CTGCCTTGTC CTTCATTGAG TGCGGCGTCG CCCTGGAGTC GGAGAGCCCC
    GCGTCCAAGT CGGCGTACTC CATCTACTCG GAGACCGTGG ACCTCATTAA GTTCATACTG
    TCATTAAAAT CCTTCTCAGA TGCCACAACG CCAACACAGG AGAAGATATT TGCTGTTCTA
    TGCATGCGTT GCCAGTCCAT TTTGAACATG GCAATGTTTC GTTGTAAAAA AGATATGGCG
    ATAAAGTATT CCCGCACGCT TAACGAACAC TTCTTCAAGA CTTCTTCCAA GGTTGCCCAG
    GCACCTTCTC CATGCATCGC AAGAAGCACA GGCACACCGT CCCCCCTCTC TCCGATCCCT
    TCTCCGGCCA GCTCCATAGG GTCCCAGTCA AGCGCTGGCA GCGTGGGGAG CGGGGCGCTG
    CCCGCCACCA TCAGCACCCC CGTCACCATC CAGAACATGA CATCCTCCTA CGTCAGCATC
    ACTTCCCACG TCCTTACCGC CTTTGACCTG TGGGAACAGG CTGAGGTCCT TACCAGGAAG
    AACAAAGAAT TCTTTGCTCA GCTCAGCACA AGTGTGTGCG CGCTGGCCCT CAACAGCAGT
    TTGATGGATC TGGTGCATTA TACAAGACAG GGTTTCCAGC GGCTAAAGCA AGTAACCAAG
    ACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235458
    Clone ID Related Accession (Same CDS sequence) XM_005208213.4
    Accession Version XM_005208213.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208213.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCCC AGTCAAGCTT GTACAATGAA GACAGAAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC TCACCAAGAT AAAGAGGCAT TTTCTGAAAA GATCCCCCTT
    TTTGGAGAGC CATACAAGAC AGAGAAAGGT GATGAGCTAT CCAGTCGGAT ACAGAACATG
    CTGGGAAACT ATGAAGAGAT GAAGGAGTTC CTTAGTAGCA AGTCCCACCC CCAGCGCTTG
    GATGCTTCTG AAAATAGGCT GGGGAAGCCG AGATACCCTT TATTTCCTGA GAAGGGGAGC
    AGCCTTCCAC CCCACTCCTT CCACACCAGT GTCCACCACC AGCCCGTTAA CACTCCTGCC
    TGTGGGCCAC ATCCTGCTGG TAACATCGGC CACCACCCAA AGATGGCGCA GCCAAGAATG
    GAACCAATGC CAAGTCTCCA CGCCAAAAGC TATGGCCCAC CGGACAGCCA GCACCTGACC
    CAAGAGCGCC TCGGTCAGGA GGGGTACGGC TCTAGTCATC ACAAAAAGGG TGACCGCAGG
    GCTGATGGAG ACCACTGTGC TTTGACGACA GACTCTGCTC CGGAGAGGGA ACTTTCACCT
    CTGTTCTCTT TGCCTTCCCC AGTCCCCCCT TTGTCACCTG TACATTCCAA CCAGCAGACT
    GCTCCCAGGA CGCAAGGAAG CGGCAAAGTT CCCAGCAGTA GCAGTAGCAA TAAAGGCTAC
    TGCCTGGCCA AGTCTCCCAA GGACCTAGCG GTAAAGGTGC ACGATAAAGA GACCCCTCAA
    GACAGCACAG GGGCAGTGGC CAGCCTTGGG GTAGCCCCTC CTCAGCCACC TTCTCAGACC
    TTCCCCCCAC CCTCCCTCCC CTCAAAAAGC CTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGACGGTCA AGACCAGGCT CCTAGCGAGT CTCCTGAACT GAAACCCCTG
    CCGGAGGAGT ACCGGCAGCA GACCTTTGAG AAATCAGACT TAAAAGTGCC TGCCAAAGCC
    AAGCTCACGA AGCTGAAAAT GCCTTCTCAG TCAGTCGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCACTCAT GGCCTCCTCC TTTGACAGCA
    ATACACACGC CTAGTACAGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG
    CACATCAGTT CTGCGGTCCC AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACAGT
    AATTCTCAGC AGGGAACATC CATGCTCAAA GATGACCTTC AGCTCAGTGA CAGTGAGGAC
    AGTGACAGCG ATCAAACCTC AGAGAAGCCT CCTTTCGCAC CTGCACCTCC AAGCGCTCCA
    CCGGCGCTGC CAGCAGCCGC GGCCTCAGCA CATTCCAGCA GCGCCGAATC GGAGAGCACC
    AGCGACTCCG ACAGCTCCTC GGACTCGGAG AGCGAGAGCA GCTCCAGTGA CAGCGAGGAG
    AACGAACCCC GAGAAGCCCC GGCTCCCGAG CCCGAGCCTC CGACAACCAA CAAATGGCAG
    CTGGACAACT GGCTGACCAA AGTCAGCCAG CCCGCGGTGC CCACCGAGGC CCCGGGTAGC
    ACGGAGCCCC CGGCCCGACC CCCGGACACT AAGGGCAAGG GCGGCGACAG CGCCACCGCG
    GGTCACGAGC GCCCAGAGTC CAAAGAGCCT CCCCCCAAAG GCTCCAGCAA AGCCCCCCGG
    GTCCCATCCG AAGGCCCCCA CGCGGGCAAG AGGAGCTGTC AGAAGTCCCC TACCCAGCAG
    GAGCCCCCCC AGCGGCAAAC CGTGGGAAGC AAACAGCCGA AAAAGCCCAT CAAGGCTTCC
    GCCCCCGGGC ACGCGGATGC GCCTGCAGAT GCGCGTGCCT GCCTGCAGGT GGAGAGCGAG
    CCCGGACTTC CTCCCCCGGC CTCCAGGGAC CAGCCCTCCA AAGACAAGCC CAAGGTGAAG
    ACGAAAGGGC GGCCCCGCGC CGCAGACAGC CGGGAGCCCA AGCCGGCGGT GCCCACCCCC
    AGCGACCGGA AGAAGCACCG CAGCGGCCCC CCGAAGGCGC CCCCGAAGGA CGCGGCGGCA
    GAGGACCGGA GCCCCGAACA CTTTGCGCTC GTTCCCCTGA CCCAGAGCCA GGGCCCTGTC
    CGCGGTGGCA GCGGCGGCGC CGGCGCCAGG ACTAGTGGCT GCAGGCTGGC TGTGGTGGTC
    CAGGAGGACC GCCGCAAAGA CAAACCCCCG GTGCCTTCGA GAGACACCAA GCTGCTCTCC
    CCGCTCAGGG ACGCTCCGCC GCCACAAAGC TTGGTGGTGA AGATCACACT CGACCTCCTG
    TCTCGGATAC CCCAGCCCCC AGGGAAGGGC AGCCGCCCCA AGAAACCAGA AGACAAACAG
    CCACCAGCAG GGAAGAAGCC AGATCCTGAG AAGAGAAGCT CAGAAAACGC CAGCAAGTTA
    GCCAAAAAGA GAAAGGGTGA AGCAGAGAAA GACCACGATA GCAAGAAAGT CAGGCTGGAG
    AAGGAAGTCA AGTCACAGCC GTTGTCTTCC TCTTCCCACA AGGAATCTTC CAAATCAAAG
    ATGTCCAGGC CCTCCTCTGA GCCCTCAAAG AAGGAAATGC TTCCCCCTTC GCTCGTGTCA
    TCCTCATCCT CCCAGAAGCC AGCCAAGCCT GCACAGAAGA GGCCACGGCA GGAAGACCCC
    GGCTGCCAGG ACCCCCAAAA AAGTGCCGGT AGTGCCAAGG GCAGCCACAG AGACCCTTCT
    GCCTCCAAAC ACAGAAAAGC AGAGGGAAAG GTCTCTGGAA GCTCCACAGA GCACAAAGGA
    TCTTCTGGAG ATACTGCAAA CCGTTTTCCA GTGCCTTCTT TGCCAAATGG TAACTCCAAA
    CCAGGGAAGC CTCACGTGAA GTTTGACAAA CAACAAGCCG ATTTTCACCT GAAGGAAGCC
    AAAAGGTTGA AGGACAAAGC AGAATTAGTG ACGGACAAGG TAGGGAAGGC CTTTAAATAC
    CTGGAGGCTG CCTTGTCCTT CATTGAGTGC GGCGTCGCCC TGGAGTCGGA GAGCCCCGCG
    TCCAAGTCGG CGTACTCCAT CTACTCGGAG ACCGTGGACC TCATTAAGTT CATACTGTCA
    TTAAAATCCT TCTCAGATGC CACAACGCCA ACACAGGAGA AGATATTTGC TGTTCTATGC
    ATGCGTTGCC AGTCCATTTT GAACATGGCA ATGTTTCGTT GTAAAAAAGA TATGGCGATA
    AAGTATTCCC GCACGCTTAA CGAACACTTC TTCAAGACTT CTTCCAAGGT TGCCCAGGCA
    CCTTCTCCAT GCATCGCAAG AAGCACAGGC ACACCGTCCC CCCTCTCTCC GATCCCTTCT
    CCGGCCAGCT CCATAGGGTC CCAGTCAAGC GCTGGCAGCG TGGGGAGCGG GGCGCTGCCC
    GCCACCATCA GCACCCCCGT CACCATCCAG AACATGACAT CCTCCTACGT CAGCATCACT
    TCCCACGTCC TTACCGCCTT TGACCTGTGG GAACAGGCTG AGGTCCTTAC CAGGAAGAAC
    AAAGAATTCT TTGCTCAGCT CAGCACAAGT GTGTGCGCGC TGGCCCTCAA CAGCAGTTTG
    ATGGATCTGG TGCATTATAC AAGACAGGGT TTCCAGCGGC TAAAGCAAGT AACCAAGACA
    CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208270.2
    CDS202..3867
    Translation

    Target ORF information:

    RefSeq Version XM_005208213.4
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208213.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCCC AGTCAAGCTT GTACAATGAA GACAGAAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC TCACCAAGAT AAAGAGGCAT TTTCTGAAAA GATCCCCCTT
    TTTGGAGAGC CATACAAGAC AGAGAAAGGT GATGAGCTAT CCAGTCGGAT ACAGAACATG
    CTGGGAAACT ATGAAGAGAT GAAGGAGTTC CTTAGTAGCA AGTCCCACCC CCAGCGCTTG
    GATGCTTCTG AAAATAGGCT GGGGAAGCCG AGATACCCTT TATTTCCTGA GAAGGGGAGC
    AGCCTTCCAC CCCACTCCTT CCACACCAGT GTCCACCACC AGCCCGTTAA CACTCCTGCC
    TGTGGGCCAC ATCCTGCTGG TAACATCGGC CACCACCCAA AGATGGCGCA GCCAAGAATG
    GAACCAATGC CAAGTCTCCA CGCCAAAAGC TATGGCCCAC CGGACAGCCA GCACCTGACC
    CAAGAGCGCC TCGGTCAGGA GGGGTACGGC TCTAGTCATC ACAAAAAGGG TGACCGCAGG
    GCTGATGGAG ACCACTGTGC TTTGACGACA GACTCTGCTC CGGAGAGGGA ACTTTCACCT
    CTGTTCTCTT TGCCTTCCCC AGTCCCCCCT TTGTCACCTG TACATTCCAA CCAGCAGACT
    GCTCCCAGGA CGCAAGGAAG CGGCAAAGTT CCCAGCAGTA GCAGTAGCAA TAAAGGCTAC
    TGCCTGGCCA AGTCTCCCAA GGACCTAGCG GTAAAGGTGC ACGATAAAGA GACCCCTCAA
    GACAGCACAG GGGCAGTGGC CAGCCTTGGG GTAGCCCCTC CTCAGCCACC TTCTCAGACC
    TTCCCCCCAC CCTCCCTCCC CTCAAAAAGC CTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGACGGTCA AGACCAGGCT CCTAGCGAGT CTCCTGAACT GAAACCCCTG
    CCGGAGGAGT ACCGGCAGCA GACCTTTGAG AAATCAGACT TAAAAGTGCC TGCCAAAGCC
    AAGCTCACGA AGCTGAAAAT GCCTTCTCAG TCAGTCGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCACTCAT GGCCTCCTCC TTTGACAGCA
    ATACACACGC CTAGTACAGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG
    CACATCAGTT CTGCGGTCCC AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACAGT
    AATTCTCAGC AGGGAACATC CATGCTCAAA GATGACCTTC AGCTCAGTGA CAGTGAGGAC
    AGTGACAGCG ATCAAACCTC AGAGAAGCCT CCTTTCGCAC CTGCACCTCC AAGCGCTCCA
    CCGGCGCTGC CAGCAGCCGC GGCCTCAGCA CATTCCAGCA GCGCCGAATC GGAGAGCACC
    AGCGACTCCG ACAGCTCCTC GGACTCGGAG AGCGAGAGCA GCTCCAGTGA CAGCGAGGAG
    AACGAACCCC GAGAAGCCCC GGCTCCCGAG CCCGAGCCTC CGACAACCAA CAAATGGCAG
    CTGGACAACT GGCTGACCAA AGTCAGCCAG CCCGCGGTGC CCACCGAGGC CCCGGGTAGC
    ACGGAGCCCC CGGCCCGACC CCCGGACACT AAGGGCAAGG GCGGCGACAG CGCCACCGCG
    GGTCACGAGC GCCCAGAGTC CAAAGAGCCT CCCCCCAAAG GCTCCAGCAA AGCCCCCCGG
    GTCCCATCCG AAGGCCCCCA CGCGGGCAAG AGGAGCTGTC AGAAGTCCCC TACCCAGCAG
    GAGCCCCCCC AGCGGCAAAC CGTGGGAAGC AAACAGCCGA AAAAGCCCAT CAAGGCTTCC
    GCCCCCGGGC ACGCGGATGC GCCTGCAGAT GCGCGTGCCT GCCTGCAGGT GGAGAGCGAG
    CCCGGACTTC CTCCCCCGGC CTCCAGGGAC CAGCCCTCCA AAGACAAGCC CAAGGTGAAG
    ACGAAAGGGC GGCCCCGCGC CGCAGACAGC CGGGAGCCCA AGCCGGCGGT GCCCACCCCC
    AGCGACCGGA AGAAGCACCG CAGCGGCCCC CCGAAGGCGC CCCCGAAGGA CGCGGCGGCA
    GAGGACCGGA GCCCCGAACA CTTTGCGCTC GTTCCCCTGA CCCAGAGCCA GGGCCCTGTC
    CGCGGTGGCA GCGGCGGCGC CGGCGCCAGG ACTAGTGGCT GCAGGCTGGC TGTGGTGGTC
    CAGGAGGACC GCCGCAAAGA CAAACCCCCG GTGCCTTCGA GAGACACCAA GCTGCTCTCC
    CCGCTCAGGG ACGCTCCGCC GCCACAAAGC TTGGTGGTGA AGATCACACT CGACCTCCTG
    TCTCGGATAC CCCAGCCCCC AGGGAAGGGC AGCCGCCCCA AGAAACCAGA AGACAAACAG
    CCACCAGCAG GGAAGAAGCC AGATCCTGAG AAGAGAAGCT CAGAAAACGC CAGCAAGTTA
    GCCAAAAAGA GAAAGGGTGA AGCAGAGAAA GACCACGATA GCAAGAAAGT CAGGCTGGAG
    AAGGAAGTCA AGTCACAGCC GTTGTCTTCC TCTTCCCACA AGGAATCTTC CAAATCAAAG
    ATGTCCAGGC CCTCCTCTGA GCCCTCAAAG AAGGAAATGC TTCCCCCTTC GCTCGTGTCA
    TCCTCATCCT CCCAGAAGCC AGCCAAGCCT GCACAGAAGA GGCCACGGCA GGAAGACCCC
    GGCTGCCAGG ACCCCCAAAA AAGTGCCGGT AGTGCCAAGG GCAGCCACAG AGACCCTTCT
    GCCTCCAAAC ACAGAAAAGC AGAGGGAAAG GTCTCTGGAA GCTCCACAGA GCACAAAGGA
    TCTTCTGGAG ATACTGCAAA CCGTTTTCCA GTGCCTTCTT TGCCAAATGG TAACTCCAAA
    CCAGGGAAGC CTCACGTGAA GTTTGACAAA CAACAAGCCG ATTTTCACCT GAAGGAAGCC
    AAAAGGTTGA AGGACAAAGC AGAATTAGTG ACGGACAAGG TAGGGAAGGC CTTTAAATAC
    CTGGAGGCTG CCTTGTCCTT CATTGAGTGC GGCGTCGCCC TGGAGTCGGA GAGCCCCGCG
    TCCAAGTCGG CGTACTCCAT CTACTCGGAG ACCGTGGACC TCATTAAGTT CATACTGTCA
    TTAAAATCCT TCTCAGATGC CACAACGCCA ACACAGGAGA AGATATTTGC TGTTCTATGC
    ATGCGTTGCC AGTCCATTTT GAACATGGCA ATGTTTCGTT GTAAAAAAGA TATGGCGATA
    AAGTATTCCC GCACGCTTAA CGAACACTTC TTCAAGACTT CTTCCAAGGT TGCCCAGGCA
    CCTTCTCCAT GCATCGCAAG AAGCACAGGC ACACCGTCCC CCCTCTCTCC GATCCCTTCT
    CCGGCCAGCT CCATAGGGTC CCAGTCAAGC GCTGGCAGCG TGGGGAGCGG GGCGCTGCCC
    GCCACCATCA GCACCCCCGT CACCATCCAG AACATGACAT CCTCCTACGT CAGCATCACT
    TCCCACGTCC TTACCGCCTT TGACCTGTGG GAACAGGCTG AGGTCCTTAC CAGGAAGAAC
    AAAGAATTCT TTGCTCAGCT CAGCACAAGT GTGTGCGCGC TGGCCCTCAA CAGCAGTTTG
    ATGGATCTGG TGCATTATAC AAGACAGGGT TTCCAGCGGC TAAAGCAAGT AACCAAGACA
    CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01736
    Clone ID Related Accession (Same CDS sequence) NM_001191525.1
    Accession Version NM_001191525.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2011-10-25
    Organism Bos taurus(Cattle)
    Product AF4/FMR2 family member 1
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03061174.1 and AAFC03061173.1. On Jul 17, 2010 this sequence version replaced XM_002704634.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCCC AGTCAAGCTT GTACAATGAA GACAGAAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC TCACCAAGAT AAAGAGGCAT TTTCTGAAAA GATCCCCCTT
    TTTGGAGAGC CATACAAGAC AGAGAAAGGT GATGAGCTAT CCAGTCGGAT ACAGAACATG
    CTGGGAAACT ATGAAGAGAT GAAGGAGTTC CTTAGTAGCA AGTCCCACCC CCAGCGCTTG
    GATGCTTCTG AAAATAGGCT GGGGAAGCCG AGATACCCTT TATTTCCTGA GAAGGGGAGC
    AGCCTTCCAC CCCACTCCTT CCACACCAGT GTCCACCACC AGCCCGTTAA CACTCCTGCC
    TGTGGGCCAC ATCCTGCTGG TAACATCGGC CACCACCCAA AGATGGCGCA GCCAAGAATG
    GAACCAATGC CAAGTCTCCA CGCCAAAAGC TATGGCCCAC CGGACAGCCA GCACCTGACC
    CAAGAGCGCC TCGGTCAGGA GGGGTACGGC TCTAGTCATC ACAAAAAGGG TGACCGCAGG
    GCTGATGGAG ACCACTGTGC TTTGACGACA GACTCTGCTC CGGAGAGGGA ACTTTCACCT
    CTGTTCTCTT TGCCTTCCCC AGTCCCCCCT TTGTCACCTG TACATTCCAA CCAGCAGACT
    GCTCCCAGGA CGCAAGGAAG CGGCAAAGTT CCCAGCAGTA GCAGTAGCAA TAAAGGCTAC
    TGCCTGGCCA AGTCTCCCAA GGACCTAGCG GTAAAGGTGC ACGATAAAGA GACCCCTCAA
    GACAGCACAG GGGCAGTGGC CAGCCTTGGG GTAGCCCCTC CTCAGCCACC TTCTCAGACC
    TTCCCCCCAC CCTCCCTCCC CTCAAAAAGC CTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGACGGTCA AGACCAGGCT CCTAGCGAGT CTCCTGAACT GAAACCCCTG
    CCGGAGGAGT ACCGGCAGCA GACCTTTGAG AAATCAGACT TAAAAGTGCC TGCCAAAGCC
    AAGCTCACGA AGCTGAAAAT GCCTTCTCAG TCAGTCGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCACTCAT GGCCTCCTCC TTTGACAGCA
    ATACACACGC CTAGTACAGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG
    CACATCAGTT CTGCGGTCCC AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACAGT
    AATTCTCAGC AGGGAACATC AAGCATGCTC AAAGATGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GCGATCAAAC CTCAGAGAAG CCTCCTTTCG CACCTGCACC TCCAAGCGCT
    CCACCGGCGC TGCCAGCAGC CGCGGCCTCA GCACATTCCA GCAGCGCCGA ATCGGAGAGC
    ACCAGCGACT CCGACAGCTC CTCGGACTCG GAGAGCGAGA GCAGCTCCAG TGACAGCGAG
    GAGAACGAAC CCCGAGAAGC CCCGGCTCCC GAGCCCGAGC CTCCGACAAC CAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCCGCGG TGCCCACCGA GGCCCCGGGT
    AGCACGGAGC CCCCGGCCCG ACCCCCGGAC ACTAAGGGCA AGGGCGGCGA CAGCGCCACC
    GCGGGTCACG AGCGCCCAGA GTCCAAAGAG CCTCCCCCCA AAGGCTCCAG CAAAGCCCCC
    CGGGTCCCAT CCGAAGGCCC CCACGCGGGC AAGAGGAGCT GTCAGAAGTC CCCTACCCAG
    CAGGAGCCCC CCCAGCGGCA AACCGTGGGA AGCAAACAGC CGAAAAAGCC CATCAAGGCT
    TCCGCCCCCG GGCACGCGGA TGCGCCTGCA GATGCGCGTG CCTGCCTGCA GGTGGAGAGC
    GAGCCCGGAC TTCCTCCCCC GGCCTCCAGG GACCAGCCCT CCAAAGACAA GCCCAAGGTG
    AAGACGAAAG GGCGGCCCCG CGCCGCAGAC AGCCGGGAGC CCAAGCCGGC GGTGCCCACC
    CCCAGCGACC GGAAGAAGCA CCGCAGCGGC CCCCCGAAGG CGCCCCCGAA GGACGCGGCG
    GCAGAGGACC GGAGCCCCGA ACACTTTGCG CTCGTTCCCC TGACCCAGAG CCAGGGCCCT
    GTCCGCGGTG GCAGCGGCGG CGCCGGCGCC AGGACTAGTG GCTGCAGGCT GGCTGTGGTG
    GTCCAGGAGG ACCGCCGCAA AGACAAACCC CCGGTGCCTT CGAGAGACAC CAAGCTGCTC
    TCCCCGCTCA GGGACACTCC GCCGCCACAA AGCTTGGTGG TGAAGATCAC ACTCGACCTC
    CTGTCTCGGA TACCCCAGCC CCCAGGGAAG GGCAGCCGCC CCAAGAAACC AGAAGACAAA
    CAGCCACCAG CAGGGAAGAA GCCAGATCCT GAGAAGAGAA GCTCAGAAAA CGCCAGCAAG
    TTAGCCAAAA AGAGAAAGGG TGAAGCAGAG AAAGACCACG ATAGCAAGAA AGTCAGGCTG
    GAGAAGGAAG TCAAGTCACA GCCGTTGTCT TCCTCTTCCC ACAAGGAATC TTCCAAATCA
    AAGATGTCCA GGCCCTCCTC TGAGCCCTCA AAGAAGGAAA TGCTTCCCCC TTCGCTCGTG
    TCATCCTCAT CCTCCCAGAA GCCAGCCAAG CCTGCACAGA AGAGGCCACG GCAGGAAGAC
    CCCAGCTGCC AGGACCCCCC AAAAAGTGCC GGTAGTGCCA AGGGCAGCCA CAGAGACCCT
    TCTGCCTCCA AACACAGAAA AGCAGAGGGG AAGGTCTCTG GAAGCTCCAC AGAGCACAAA
    GGATCTTCTG GAGATACTGC AAACCGTTTT CCAGTGCCTT CTTTGCCAAA TGGTAACTCC
    AAACCAGGGA AGCCTCACGT GAAGTTTGAC AAACAACAAG CCGATTTTCA CCTGAAGGAA
    GCCAAAAGGT TGAAGGACAA AGCAGAATTA GTGACGGACA AGGTAGGGAA GGCCTTTAAA
    TACCTGGAGG CTGCCTTGTC CTTCATTGAG TGCGGCGTCG CCCTGGAGTC GGAGAGCCCC
    GCGTCCAAGT CGGCGTACTC CATCTACTCG GAGACCGTGG ACCTCATTAA GTTCATACTG
    TCATTAAAAT CCTTCTCAGA TGCCACAACG CCAACACAGG AGAAGATATT TGCTGTTCTA
    TGCATGCGTT GCCAGTCCAT TTTGAACATG GCAATGTTTC GTTGTAAAAA AGATATGGCG
    ATAAAGTATT CCCGCACGCT TAACGAACAC TTCTTCAAGA CTTCTTCCAA GGTTGCCCAG
    GCACCTTCTC CATGCATCGC AAGCACAGGC ACACCGTCCC CCCTCTCTCC GATCCCTTCT
    CCGGCCAGCT CCATAGGGTC CCAGTCAAGC GCTGGCAGCG TGGGGAGCGG GGCGCTGCCC
    GCCACCATCA GCACCCCCGT CACCATCCAG AACATGACAT CCTCCTACGT CAGCATCACT
    TCCCACGTCC TTACCGCCTT TGACCTGTGG GAACAGGCTG AGGTCCTTAC CAGGAAGAAC
    AAAGAATTCT TTGCTCAGCT CAGCACAAGT GTGTGCGCGC TGGCCCTCAA CAGCAGTTTG
    ATGGATCTGG TGCATTATAC AAGACAGGGT TTCCAGCGGC TAAAGCAAGT AACCAAGACA
    CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178454.1
    CDS176..3841
    Translation

    Target ORF information:

    RefSeq Version NM_001191525.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus AF4/FMR2 family, member 1 (AFF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191525.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCCC AGTCAAGCTT GTACAATGAA GACAGAAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC TCACCAAGAT AAAGAGGCAT TTTCTGAAAA GATCCCCCTT
    TTTGGAGAGC CATACAAGAC AGAGAAAGGT GATGAGCTAT CCAGTCGGAT ACAGAACATG
    CTGGGAAACT ATGAAGAGAT GAAGGAGTTC CTTAGTAGCA AGTCCCACCC CCAGCGCTTG
    GATGCTTCTG AAAATAGGCT GGGGAAGCCG AGATACCCTT TATTTCCTGA GAAGGGGAGC
    AGCCTTCCAC CCCACTCCTT CCACACCAGT GTCCACCACC AGCCCGTTAA CACTCCTGCC
    TGTGGGCCAC ATCCTGCTGG TAACATCGGC CACCACCCAA AGATGGCGCA GCCAAGAATG
    GAACCAATGC CAAGTCTCCA CGCCAAAAGC TATGGCCCAC CGGACAGCCA GCACCTGACC
    CAAGAGCGCC TCGGTCAGGA GGGGTACGGC TCTAGTCATC ACAAAAAGGG TGACCGCAGG
    GCTGATGGAG ACCACTGTGC TTTGACGACA GACTCTGCTC CGGAGAGGGA ACTTTCACCT
    CTGTTCTCTT TGCCTTCCCC AGTCCCCCCT TTGTCACCTG TACATTCCAA CCAGCAGACT
    GCTCCCAGGA CGCAAGGAAG CGGCAAAGTT CCCAGCAGTA GCAGTAGCAA TAAAGGCTAC
    TGCCTGGCCA AGTCTCCCAA GGACCTAGCG GTAAAGGTGC ACGATAAAGA GACCCCTCAA
    GACAGCACAG GGGCAGTGGC CAGCCTTGGG GTAGCCCCTC CTCAGCCACC TTCTCAGACC
    TTCCCCCCAC CCTCCCTCCC CTCAAAAAGC CTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGACGGTCA AGACCAGGCT CCTAGCGAGT CTCCTGAACT GAAACCCCTG
    CCGGAGGAGT ACCGGCAGCA GACCTTTGAG AAATCAGACT TAAAAGTGCC TGCCAAAGCC
    AAGCTCACGA AGCTGAAAAT GCCTTCTCAG TCAGTCGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCACTCAT GGCCTCCTCC TTTGACAGCA
    ATACACACGC CTAGTACAGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG
    CACATCAGTT CTGCGGTCCC AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACAGT
    AATTCTCAGC AGGGAACATC AAGCATGCTC AAAGATGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GCGATCAAAC CTCAGAGAAG CCTCCTTTCG CACCTGCACC TCCAAGCGCT
    CCACCGGCGC TGCCAGCAGC CGCGGCCTCA GCACATTCCA GCAGCGCCGA ATCGGAGAGC
    ACCAGCGACT CCGACAGCTC CTCGGACTCG GAGAGCGAGA GCAGCTCCAG TGACAGCGAG
    GAGAACGAAC CCCGAGAAGC CCCGGCTCCC GAGCCCGAGC CTCCGACAAC CAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCCGCGG TGCCCACCGA GGCCCCGGGT
    AGCACGGAGC CCCCGGCCCG ACCCCCGGAC ACTAAGGGCA AGGGCGGCGA CAGCGCCACC
    GCGGGTCACG AGCGCCCAGA GTCCAAAGAG CCTCCCCCCA AAGGCTCCAG CAAAGCCCCC
    CGGGTCCCAT CCGAAGGCCC CCACGCGGGC AAGAGGAGCT GTCAGAAGTC CCCTACCCAG
    CAGGAGCCCC CCCAGCGGCA AACCGTGGGA AGCAAACAGC CGAAAAAGCC CATCAAGGCT
    TCCGCCCCCG GGCACGCGGA TGCGCCTGCA GATGCGCGTG CCTGCCTGCA GGTGGAGAGC
    GAGCCCGGAC TTCCTCCCCC GGCCTCCAGG GACCAGCCCT CCAAAGACAA GCCCAAGGTG
    AAGACGAAAG GGCGGCCCCG CGCCGCAGAC AGCCGGGAGC CCAAGCCGGC GGTGCCCACC
    CCCAGCGACC GGAAGAAGCA CCGCAGCGGC CCCCCGAAGG CGCCCCCGAA GGACGCGGCG
    GCAGAGGACC GGAGCCCCGA ACACTTTGCG CTCGTTCCCC TGACCCAGAG CCAGGGCCCT
    GTCCGCGGTG GCAGCGGCGG CGCCGGCGCC AGGACTAGTG GCTGCAGGCT GGCTGTGGTG
    GTCCAGGAGG ACCGCCGCAA AGACAAACCC CCGGTGCCTT CGAGAGACAC CAAGCTGCTC
    TCCCCGCTCA GGGACACTCC GCCGCCACAA AGCTTGGTGG TGAAGATCAC ACTCGACCTC
    CTGTCTCGGA TACCCCAGCC CCCAGGGAAG GGCAGCCGCC CCAAGAAACC AGAAGACAAA
    CAGCCACCAG CAGGGAAGAA GCCAGATCCT GAGAAGAGAA GCTCAGAAAA CGCCAGCAAG
    TTAGCCAAAA AGAGAAAGGG TGAAGCAGAG AAAGACCACG ATAGCAAGAA AGTCAGGCTG
    GAGAAGGAAG TCAAGTCACA GCCGTTGTCT TCCTCTTCCC ACAAGGAATC TTCCAAATCA
    AAGATGTCCA GGCCCTCCTC TGAGCCCTCA AAGAAGGAAA TGCTTCCCCC TTCGCTCGTG
    TCATCCTCAT CCTCCCAGAA GCCAGCCAAG CCTGCACAGA AGAGGCCACG GCAGGAAGAC
    CCCAGCTGCC AGGACCCCCC AAAAAGTGCC GGTAGTGCCA AGGGCAGCCA CAGAGACCCT
    TCTGCCTCCA AACACAGAAA AGCAGAGGGG AAGGTCTCTG GAAGCTCCAC AGAGCACAAA
    GGATCTTCTG GAGATACTGC AAACCGTTTT CCAGTGCCTT CTTTGCCAAA TGGTAACTCC
    AAACCAGGGA AGCCTCACGT GAAGTTTGAC AAACAACAAG CCGATTTTCA CCTGAAGGAA
    GCCAAAAGGT TGAAGGACAA AGCAGAATTA GTGACGGACA AGGTAGGGAA GGCCTTTAAA
    TACCTGGAGG CTGCCTTGTC CTTCATTGAG TGCGGCGTCG CCCTGGAGTC GGAGAGCCCC
    GCGTCCAAGT CGGCGTACTC CATCTACTCG GAGACCGTGG ACCTCATTAA GTTCATACTG
    TCATTAAAAT CCTTCTCAGA TGCCACAACG CCAACACAGG AGAAGATATT TGCTGTTCTA
    TGCATGCGTT GCCAGTCCAT TTTGAACATG GCAATGTTTC GTTGTAAAAA AGATATGGCG
    ATAAAGTATT CCCGCACGCT TAACGAACAC TTCTTCAAGA CTTCTTCCAA GGTTGCCCAG
    GCACCTTCTC CATGCATCGC AAGCACAGGC ACACCGTCCC CCCTCTCTCC GATCCCTTCT
    CCGGCCAGCT CCATAGGGTC CCAGTCAAGC GCTGGCAGCG TGGGGAGCGG GGCGCTGCCC
    GCCACCATCA GCACCCCCGT CACCATCCAG AACATGACAT CCTCCTACGT CAGCATCACT
    TCCCACGTCC TTACCGCCTT TGACCTGTGG GAACAGGCTG AGGTCCTTAC CAGGAAGAAC
    AAAGAATTCT TTGCTCAGCT CAGCACAAGT GTGTGCGCGC TGGCCCTCAA CAGCAGTTTG
    ATGGATCTGG TGCATTATAC AAGACAGGGT TTCCAGCGGC TAAAGCAAGT AACCAAGACA
    CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216353
    Clone ID Related Accession (Same CDS sequence) NM_001191525.2
    Accession Version NM_001191525.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02018350.1, DAAA02018351.1 and DAAA02018352.1. On Jun 30, 2017 this sequence version replaced NM_001191525.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02403646, SAMN02403652 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCCC AGTCAAGCTT GTACAATGAA GACAGAAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC TCACCAAGAT AAAGAGGCAT TTTCTGAAAA GATCCCCCTT
    TTTGGAGAGC CATACAAGAC AGAGAAAGGT GATGAGCTAT CCAGTCGGAT ACAGAACATG
    CTGGGAAACT ATGAAGAGAT GAAGGAGTTC CTTAGTAGCA AGTCCCACCC CCAGCGCTTG
    GATGCTTCTG AAAATAGGCT GGGGAAGCCG AGATACCCTT TATTTCCTGA GAAGGGGAGC
    AGCCTTCCAC CCCACTCCTT CCACACCAGT GTCCACCACC AGCCCGTTAA CACTCCTGCC
    TGTGGGCCAC ATCCTGCTGG TAACATCGGC CACCACCCAA AGATGGCGCA GCCAAGAATG
    GAACCAATGC CAAGTCTCCA CGCCAAAAGC TATGGCCCAC CGGACAGCCA GCACCTGACC
    CAAGAGCGCC TCGGTCAGGA GGGGTACGGC TCTAGTCATC ACAAAAAGGG TGACCGCAGG
    GCTGATGGAG ACCACTGTGC TTTGACGACA GACTCTGCTC CGGAGAGGGA ACTTTCACCT
    CTGTTCTCTT TGCCTTCCCC AGTCCCCCCT TTGTCACCTG TACATTCCAA CCAGCAGACT
    GCTCCCAGGA CGCAAGGAAG CGGCAAAGTT CCCAGCAGTA GCAGTAGCAA TAAAGGCTAC
    TGCCTGGCCA AGTCTCCCAA GGACCTAGCG GTAAAGGTGC ACGATAAAGA GACCCCTCAA
    GACAGCACAG GGGCAGTGGC CAGCCTTGGG GTAGCCCCTC CTCAGCCACC TTCTCAGACC
    TTCCCCCCAC CCTCCCTCCC CTCAAAAAGC CTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGACGGTCA AGACCAGGCT CCTAGCGAGT CTCCTGAACT GAAACCCCTG
    CCGGAGGAGT ACCGGCAGCA GACCTTTGAG AAATCAGACT TAAAAGTGCC TGCCAAAGCC
    AAGCTCACGA AGCTGAAAAT GCCTTCTCAG TCAGTCGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCACTCAT GGCCTCCTCC TTTGACAGCA
    ATACACACGC CTAGTACAGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG
    CACATCAGTT CTGCGGTCCC AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACAGT
    AATTCTCAGC AGGGAACATC AAGCATGCTC AAAGATGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GCGATCAAAC CTCAGAGAAG CCTCCTTTCG CACCTGCACC TCCAAGCGCT
    CCACCGGCGC TGCCAGCAGC CGCGGCCTCA GCACATTCCA GCAGCGCCGA ATCGGAGAGC
    ACCAGCGACT CCGACAGCTC CTCGGACTCG GAGAGCGAGA GCAGCTCCAG TGACAGCGAG
    GAGAACGAAC CCCGAGAAGC CCCGGCTCCC GAGCCCGAGC CTCCGACAAC CAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCCGCGG TGCCCACCGA GGCCCCGGGT
    AGCACGGAGC CCCCGGCCCG ACCCCCGGAC ACTAAGGGCA AGGGCGGCGA CAGCGCCACC
    GCGGGTCACG AGCGCCCAGA GTCCAAAGAG CCTCCCCCCA AAGGCTCCAG CAAAGCCCCC
    CGGGTCCCAT CCGAAGGCCC CCACGCGGGC AAGAGGAGCT GTCAGAAGTC CCCTGCCCAG
    CAGGAGCCCC CCCAGCGGCA AACCGTGGGA AGCAAACAGC CGAAAAAGCC CATCAAGGCT
    TCCGCCCCCG GGCACGCGGA TGCGCCTGCA GATGCGCGTG CCTGCCTGCA GGTGGAGAGC
    GAGCCCGGAC TTCCTCCCCC GGCCTCCAGG GACCAGCCCT CCAAAGACAA GCCCAAGGTG
    AAGACGAAAG GGCGGCCCCG CGCCGCAGAC AGCCGGGAGC CCAAGCCGGC GGTGCCCACC
    CCCAGCGACC GGAAGAAGCA CCGCAGCGGC CCCCCGAAGG CGCCCCCGAA GGACGCGGCG
    GCAGAGGACC GGAGCCCCGA ACACTTTGCG CTCGTTCCCC TGACCCAGAG CCAGGGCCCT
    GTCCGCGGTG GCAGCGGCGG CGCCGGCGCC AGGACTAGTG GCTGCAGGCT GGCTGTGGTG
    GTCCAGGAGG ACCGCCGCAA AGACAAACCC CCGGTGCCTT CGAGAGACAC CAAGCTGCTC
    TCCCCGCTCA GGGACACTCC GCCGCCACAA AGCTTGGTGG TGAAGATCAC ACTCGACCTC
    CTGTCTCGGA TACCCCAGCC CCCAGGGAAG GGCAGCCGCC CCAAGAAACC AGAAGACAAA
    CAGCCACCAG CAGGGAAGAA GCCAGATCCT GAGAAGAGAA GCTCAGAAAA CGCCAGCAAG
    TTAGCCAAAA AGAGAAAGGG TGAAGCAGAG AAAGACCACG ATAGCAAGAA AGTCAGGCTG
    GAGAAGGAAG TCAAGTCACA GCCGTTGTCT TCCTCTTCCC ACAAGGAATC TTCCAAATCA
    AAGATGTCCA GGCCCTCCTC TGAGCCCTCA AAGAAGGAAA TGCTTCCCCC TTCGCTCGTG
    TCATCCTCAT CCTCCCAGAA GCCAGCCAAG CCTGCACAGA AGAGGCCACG GCAGGAAGAC
    CCCGGCTGCC AGGACCCCCA AAAAAGTGCC GGTAGTGCCA AGGGCAGCCA CAGAGACCCT
    TCTGCCTCCA AACACAGAAA AGCAGAGGGA AAGGTCTCTG GAAGCTCCAC AGAGCACAAA
    GGATCTTCTG GAGATACTGC AAACCGTTTT CCAGTGCCTT CTTTGCCAAA TGGTAACTCC
    AAACCAGGGA AGCCTCACGT GAAGTTTGAC AAACAACAAG CCGATTTTCA CCTGAAGGAA
    GCCAAAAGGT TGAAGGACAA AGCAGAATTA GTGACGGACA AGGTAGGGAA GGCCTTTAAA
    TACCTGGAGG CTGCCTTGTC CTTCATTGAG TGCGGCGTCG CCCTGGAGTC GGAGAGCCCC
    GCGTCCAAGT CGGCGTACTC CATCTACTCG GAGACCGTGG ACCTCATTAA GTTCATACTG
    TCATTAAAAT CCTTCTCAGA TGCCACAACG CCAACACAGG AGAAGATATT TGCTGTTCTA
    TGCATGCGTT GCCAGTCCAT TTTGAACATG GCAATGTTTC GTTGTAAAAA AGATATGGCG
    ATAAAGTATT CCCGCACGCT TAACGAACAC TTCTTCAAGA CTTCTTCCAA GGTTGCCCAG
    GCACCTTCTC CATGCATCGC AAGCACAGGC ACACCGTCCC CCCTCTCTCC GATCCCTTCT
    CCGGCCAGCT CCATAGGGTC CCAGTCAAGC GCTGGCAGCG TGGGGAGCGG GGCGCTGCCC
    GCCACCATCA GCACCCCCGT CACCATCCAG AACATGACAT CCTCCTACGT CAGCATCACT
    TCCCACGTCC TTACCGCCTT TGACCTGTGG GAACAGGCTG AGGTCCTTAC CAGGAAGAAC
    AAAGAATTCT TTGCTCAGCT CAGCACAAGT GTGTGCGCGC TGGCCCTCAA CAGCAGTTTG
    ATGGATCTGG TGCATTATAC AAGACAGGGT TTCCAGCGGC TAAAGCAAGT AACCAAGACA
    CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178454.2
    CDS176..3841
    Translation

    Target ORF information:

    RefSeq Version NM_001191525.2
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 1 (AFF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191525.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCCC AGTCAAGCTT GTACAATGAA GACAGAAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC TCACCAAGAT AAAGAGGCAT TTTCTGAAAA GATCCCCCTT
    TTTGGAGAGC CATACAAGAC AGAGAAAGGT GATGAGCTAT CCAGTCGGAT ACAGAACATG
    CTGGGAAACT ATGAAGAGAT GAAGGAGTTC CTTAGTAGCA AGTCCCACCC CCAGCGCTTG
    GATGCTTCTG AAAATAGGCT GGGGAAGCCG AGATACCCTT TATTTCCTGA GAAGGGGAGC
    AGCCTTCCAC CCCACTCCTT CCACACCAGT GTCCACCACC AGCCCGTTAA CACTCCTGCC
    TGTGGGCCAC ATCCTGCTGG TAACATCGGC CACCACCCAA AGATGGCGCA GCCAAGAATG
    GAACCAATGC CAAGTCTCCA CGCCAAAAGC TATGGCCCAC CGGACAGCCA GCACCTGACC
    CAAGAGCGCC TCGGTCAGGA GGGGTACGGC TCTAGTCATC ACAAAAAGGG TGACCGCAGG
    GCTGATGGAG ACCACTGTGC TTTGACGACA GACTCTGCTC CGGAGAGGGA ACTTTCACCT
    CTGTTCTCTT TGCCTTCCCC AGTCCCCCCT TTGTCACCTG TACATTCCAA CCAGCAGACT
    GCTCCCAGGA CGCAAGGAAG CGGCAAAGTT CCCAGCAGTA GCAGTAGCAA TAAAGGCTAC
    TGCCTGGCCA AGTCTCCCAA GGACCTAGCG GTAAAGGTGC ACGATAAAGA GACCCCTCAA
    GACAGCACAG GGGCAGTGGC CAGCCTTGGG GTAGCCCCTC CTCAGCCACC TTCTCAGACC
    TTCCCCCCAC CCTCCCTCCC CTCAAAAAGC CTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGACGGTCA AGACCAGGCT CCTAGCGAGT CTCCTGAACT GAAACCCCTG
    CCGGAGGAGT ACCGGCAGCA GACCTTTGAG AAATCAGACT TAAAAGTGCC TGCCAAAGCC
    AAGCTCACGA AGCTGAAAAT GCCTTCTCAG TCAGTCGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCACTCAT GGCCTCCTCC TTTGACAGCA
    ATACACACGC CTAGTACAGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG
    CACATCAGTT CTGCGGTCCC AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACAGT
    AATTCTCAGC AGGGAACATC AAGCATGCTC AAAGATGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GCGATCAAAC CTCAGAGAAG CCTCCTTTCG CACCTGCACC TCCAAGCGCT
    CCACCGGCGC TGCCAGCAGC CGCGGCCTCA GCACATTCCA GCAGCGCCGA ATCGGAGAGC
    ACCAGCGACT CCGACAGCTC CTCGGACTCG GAGAGCGAGA GCAGCTCCAG TGACAGCGAG
    GAGAACGAAC CCCGAGAAGC CCCGGCTCCC GAGCCCGAGC CTCCGACAAC CAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCCGCGG TGCCCACCGA GGCCCCGGGT
    AGCACGGAGC CCCCGGCCCG ACCCCCGGAC ACTAAGGGCA AGGGCGGCGA CAGCGCCACC
    GCGGGTCACG AGCGCCCAGA GTCCAAAGAG CCTCCCCCCA AAGGCTCCAG CAAAGCCCCC
    CGGGTCCCAT CCGAAGGCCC CCACGCGGGC AAGAGGAGCT GTCAGAAGTC CCCTGCCCAG
    CAGGAGCCCC CCCAGCGGCA AACCGTGGGA AGCAAACAGC CGAAAAAGCC CATCAAGGCT
    TCCGCCCCCG GGCACGCGGA TGCGCCTGCA GATGCGCGTG CCTGCCTGCA GGTGGAGAGC
    GAGCCCGGAC TTCCTCCCCC GGCCTCCAGG GACCAGCCCT CCAAAGACAA GCCCAAGGTG
    AAGACGAAAG GGCGGCCCCG CGCCGCAGAC AGCCGGGAGC CCAAGCCGGC GGTGCCCACC
    CCCAGCGACC GGAAGAAGCA CCGCAGCGGC CCCCCGAAGG CGCCCCCGAA GGACGCGGCG
    GCAGAGGACC GGAGCCCCGA ACACTTTGCG CTCGTTCCCC TGACCCAGAG CCAGGGCCCT
    GTCCGCGGTG GCAGCGGCGG CGCCGGCGCC AGGACTAGTG GCTGCAGGCT GGCTGTGGTG
    GTCCAGGAGG ACCGCCGCAA AGACAAACCC CCGGTGCCTT CGAGAGACAC CAAGCTGCTC
    TCCCCGCTCA GGGACACTCC GCCGCCACAA AGCTTGGTGG TGAAGATCAC ACTCGACCTC
    CTGTCTCGGA TACCCCAGCC CCCAGGGAAG GGCAGCCGCC CCAAGAAACC AGAAGACAAA
    CAGCCACCAG CAGGGAAGAA GCCAGATCCT GAGAAGAGAA GCTCAGAAAA CGCCAGCAAG
    TTAGCCAAAA AGAGAAAGGG TGAAGCAGAG AAAGACCACG ATAGCAAGAA AGTCAGGCTG
    GAGAAGGAAG TCAAGTCACA GCCGTTGTCT TCCTCTTCCC ACAAGGAATC TTCCAAATCA
    AAGATGTCCA GGCCCTCCTC TGAGCCCTCA AAGAAGGAAA TGCTTCCCCC TTCGCTCGTG
    TCATCCTCAT CCTCCCAGAA GCCAGCCAAG CCTGCACAGA AGAGGCCACG GCAGGAAGAC
    CCCGGCTGCC AGGACCCCCA AAAAAGTGCC GGTAGTGCCA AGGGCAGCCA CAGAGACCCT
    TCTGCCTCCA AACACAGAAA AGCAGAGGGA AAGGTCTCTG GAAGCTCCAC AGAGCACAAA
    GGATCTTCTG GAGATACTGC AAACCGTTTT CCAGTGCCTT CTTTGCCAAA TGGTAACTCC
    AAACCAGGGA AGCCTCACGT GAAGTTTGAC AAACAACAAG CCGATTTTCA CCTGAAGGAA
    GCCAAAAGGT TGAAGGACAA AGCAGAATTA GTGACGGACA AGGTAGGGAA GGCCTTTAAA
    TACCTGGAGG CTGCCTTGTC CTTCATTGAG TGCGGCGTCG CCCTGGAGTC GGAGAGCCCC
    GCGTCCAAGT CGGCGTACTC CATCTACTCG GAGACCGTGG ACCTCATTAA GTTCATACTG
    TCATTAAAAT CCTTCTCAGA TGCCACAACG CCAACACAGG AGAAGATATT TGCTGTTCTA
    TGCATGCGTT GCCAGTCCAT TTTGAACATG GCAATGTTTC GTTGTAAAAA AGATATGGCG
    ATAAAGTATT CCCGCACGCT TAACGAACAC TTCTTCAAGA CTTCTTCCAA GGTTGCCCAG
    GCACCTTCTC CATGCATCGC AAGCACAGGC ACACCGTCCC CCCTCTCTCC GATCCCTTCT
    CCGGCCAGCT CCATAGGGTC CCAGTCAAGC GCTGGCAGCG TGGGGAGCGG GGCGCTGCCC
    GCCACCATCA GCACCCCCGT CACCATCCAG AACATGACAT CCTCCTACGT CAGCATCACT
    TCCCACGTCC TTACCGCCTT TGACCTGTGG GAACAGGCTG AGGTCCTTAC CAGGAAGAAC
    AAAGAATTCT TTGCTCAGCT CAGCACAAGT GTGTGCGCGC TGGCCCTCAA CAGCAGTTTG
    ATGGATCTGG TGCATTATAC AAGACAGGGT TTCCAGCGGC TAAAGCAAGT AACCAAGACA
    CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20595
    Clone ID Related Accession (Same CDS sequence) XM_010806299.2 , XM_005208211.3
    Accession Version XM_010806299.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103983.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806299.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CCGAAAGAGT CAACAGCAGC AGCAACAGCA GCTTGTACAA TGAAGACAGA 
    AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGATAAAGAG
    GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCATACA AGACAGAGAA AGGTGATGAG
    CTATCCAGTC GGATACAGAA CATGCTGGGA AACTATGAAG AGATGAAGGA GTTCCTTAGT
    AGCAAGTCCC ACCCCCAGCG CTTGGATGCT TCTGAAAATA GGCTGGGGAA GCCGAGATAC
    CCTTTATTTC CTGAGAAGGG GAGCAGCCTT CCACCCCACT CCTTCCACAC CAGTGTCCAC
    CACCAGCCCG TTAACACTCC TGCCTGTGGG CCACATCCTG CTGGTAACAT CGGCCACCAC
    CCAAAGATGG CGCAGCCAAG AATGGAACCA ATGCCAAGTC TCCACGCCAA AAGCTATGGC
    CCACCGGACA GCCAGCACCT GACCCAAGAG CGCCTCGGTC AGGAGGGGTA CGGCTCTAGT
    CATCACAAAA AGGGTGACCG CAGGGCTGAT GGAGACCACT GTGCTTTGAC GACAGACTCT
    GCTCCGGAGA GGGAACTTTC ACCTCTGTTC TCTTTGCCTT CCCCAGTCCC CCCTTTGTCA
    CCTGTACATT CCAACCAGCA GACTGCTCCC AGGACGCAAG GAAGCGGCAA AGTTCCCAGC
    AGTAGCAGTA GCAATAAAGG CTACTGCCTG GCCAAGTCTC CCAAGGACCT AGCGGTAAAG
    GTGCACGATA AAGAGACCCC TCAAGACAGC ACAGGGGCAG TGGCCAGCCT TGGGGTAGCC
    CCTCCTCAGC CACCTTCTCA GACCTTCCCC CCACCCTCCC TCCCCTCAAA AAGCCTTGCA
    ATGCAGCAGA AGCCCACGGC TTATGTCCGG CCCATGGACG GTCAAGACCA GGCTCCTAGC
    GAGTCTCCTG AACTGAAACC CCTGCCGGAG GAGTACCGGC AGCAGACCTT TGAGAAATCA
    GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACGAAGCTGA AAATGCCTTC TCAGTCAGTC
    GAGCAGACCT ACTCCAATGA AGTCCATTGT GTTGAAGAGA TTCTGAAGGA AATGACCCAC
    TCATGGCCTC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CAGCTGAGCC CTCCAAATTT
    CCTTTTCCCA CAAAGGATTC TCAGCACATC AGTTCTGCGG TCCCAAACCA AAAACAATAT
    GATACATCTT CAAAAACTCA CAGTAATTCT CAGCAGGGAA CATCCATGCT CAAAGATGAC
    CTTCAGCTCA GTGACAGTGA GGACAGTGAC AGCGATCAAA CCTCAGAGAA GCCTCCTTTC
    GCACCTGCAC CTCCAAGCGC TCCACCGGCG CTGCCAGCAG CCGCGGCCTC AGCACATTCC
    AGCAGCGCCG AATCGGAGAG CACCAGCGAC TCCGACAGCT CCTCGGACTC GGAGAGCGAG
    AGCAGCTCCA GTGACAGCGA GGAGAACGAA CCCCGAGAAG CCCCGGCTCC CGAGCCCGAG
    CCTCCGACAA CCAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAGCCCGCG
    GTGCCCACCG AGGCCCCGGG TAGCACGGAG CCCCCGGCCC GACCCCCGGA CACTAAGGGC
    AAGGGCGGCG ACAGCGCCAC CGCGGGTCAC GAGCGCCCAG AGTCCAAAGA GCCTCCCCCC
    AAAGGCTCCA GCAAAGCCCC CCGGGTCCCA TCCGAAGGCC CCCACGCGGG CAAGAGGAGC
    TGTCAGAAGT CCCCTGCCCA GCAGGAGCCC CCCCAGCGGC AAACCGTGGG AAGCAAACAG
    CCGAAAAAGC CCATCAAGGC TTCCGCCCCC GGGCACGCGG ATGCGCCTGC AGATGCGCGT
    GCCTGCCTGC AGGTGGAGAG CGAGCCCGGA CTTCCTCCCC CGGCCTCCAG GGACCAGCCC
    TCCAAAGACA AGCCCAAGGT GAAGACGAAA GGGCGGCCCC GCGCCGCAGA CAGCCGGGAG
    CCCAAGCCGG CGGTGCCCAC CCCCAGCGAC CGGAAGAAGC ACCGCAGCGG CCCCCCGAAG
    GCGCCCCCGA AGGACGCGGC GGCAGAGGAC CGGAGCCCCG AACACTTTGC GCTCGTTCCC
    CTGACCCAGA GCCAGGGCCC TGTCCGCGGT GGCAGCGGCG GCGCCGGCGC CAGGACTAGT
    GGCTGCAGGC TGGCTGTGGT GGTCCAGGAG GACCGCCGCA AAGACAAACC CCCGGTGCCT
    TCGAGAGACA CCAAGCTGCT CTCCCCGCTC AGGGACACTC CGCCGCCACA AAGCTTGGTG
    GTGAAGATCA CACTCGACCT CCTGTCTCGG ATACCCCAGC CCCCAGGGAA GGGCAGCCGC
    CCCAAGAAAC CAGAAGACAA ACAGCCACCA GCAGGGAAGA AGCCAGATCC TGAGAAGAGA
    AGCTCAGAAA ACGCCAGCAA GTTAGCCAAA AAGAGAAAGG GTGAAGCAGA GAAAGACCAC
    GATAGCAAGA AAGTCAGGCT GGAGAAGGAA GTCAAGTCAC AGCCGTTGTC TTCCTCTTCC
    CACAAGGAAT CTTCCAAATC AAAGATGTCC AGGCCCTCCT CTGAGCCCTC AAAGAAGGAA
    ATGCTTCCCC CTTCGCTCGT GTCATCCTCA TCCTCCCAGA AGCCAGCCAA GCCTGCACAG
    AAGAGGCCAC GGCAGGAAGA CCCCGGCTGC CAGGACCCCC AAAAAAGTGC CGGTAGTGCC
    AAGGGCAGCC ACAGAGACCC TTCTGCCTCC AAACACAGAA AAGCAGAGGG AAAGGTCTCT
    GGAAGCTCCA CAGAGCACAA AGGATCTTCT GGAGATACTG CAAACCGTTT TCCAGTGCCT
    TCTTTGCCAA ATGGTAACTC CAAACCAGGG AAGCCTCACG TGAAGTTTGA CAAACAACAA
    GCCGATTTTC ACCTGAAGGA AGCCAAAAGG TTGAAGGACA AAGCAGAATT AGTGACGGAC
    AAGGTAGGGA AGGCCTTTAA ATACCTGGAG GCTGCCTTGT CCTTCATTGA GTGCGGCGTC
    GCCCTGGAGT CGGAGAGCCC CGCGTCCAAG TCGGCGTACT CCATCTACTC GGAGACCGTG
    GACCTCATTA AGTTCATACT GTCATTAAAA TCCTTCTCAG ATGCCACAAC GCCAACACAG
    GAGAAGATAT TTGCTGTTCT ATGCATGCGT TGCCAGTCCA TTTTGAACAT GGCAATGTTT
    CGTTGTAAAA AAGATATGGC GATAAAGTAT TCCCGCACGC TTAACGAACA CTTCTTCAAG
    ACTTCTTCCA AGGTTGCCCA GGCACCTTCT CCATGCATCG CAAGAAGCAC AGGCACACCG
    TCCCCCCTCT CTCCGATCCC TTCTCCGGCC AGCTCCATAG GGTCCCAGTC AAGCGCTGGC
    AGCGTGGGGA GCGGGGCGCT GCCCGCCACC ATCAGCACCC CCGTCACCAT CCAGAACATG
    ACATCCTCCT ACGTCAGCAT CACTTCCCAC GTCCTTACCG CCTTTGACCT GTGGGAACAG
    GCTGAGGTCC TTACCAGGAA GAACAAAGAA TTCTTTGCTC AGCTCAGCAC AAGTGTGTGC
    GCGCTGGCCC TCAACAGCAG TTTGATGGAT CTGGTGCATT ATACAAGACA GGGTTTCCAG
    CGGCTAAAGC AAGTAACCAA GACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804601.1
    CDS136..3825
    Translation

    Target ORF information:

    RefSeq Version XM_010806299.2
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806299.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CCGAAAGAGT CAACAGCAGC AGCAACAGCA GCTTGTACAA TGAAGACAGA 
    AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGATAAAGAG
    GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCATACA AGACAGAGAA AGGTGATGAG
    CTATCCAGTC GGATACAGAA CATGCTGGGA AACTATGAAG AGATGAAGGA GTTCCTTAGT
    AGCAAGTCCC ACCCCCAGCG CTTGGATGCT TCTGAAAATA GGCTGGGGAA GCCGAGATAC
    CCTTTATTTC CTGAGAAGGG GAGCAGCCTT CCACCCCACT CCTTCCACAC CAGTGTCCAC
    CACCAGCCCG TTAACACTCC TGCCTGTGGG CCACATCCTG CTGGTAACAT CGGCCACCAC
    CCAAAGATGG CGCAGCCAAG AATGGAACCA ATGCCAAGTC TCCACGCCAA AAGCTATGGC
    CCACCGGACA GCCAGCACCT GACCCAAGAG CGCCTCGGTC AGGAGGGGTA CGGCTCTAGT
    CATCACAAAA AGGGTGACCG CAGGGCTGAT GGAGACCACT GTGCTTTGAC GACAGACTCT
    GCTCCGGAGA GGGAACTTTC ACCTCTGTTC TCTTTGCCTT CCCCAGTCCC CCCTTTGTCA
    CCTGTACATT CCAACCAGCA GACTGCTCCC AGGACGCAAG GAAGCGGCAA AGTTCCCAGC
    AGTAGCAGTA GCAATAAAGG CTACTGCCTG GCCAAGTCTC CCAAGGACCT AGCGGTAAAG
    GTGCACGATA AAGAGACCCC TCAAGACAGC ACAGGGGCAG TGGCCAGCCT TGGGGTAGCC
    CCTCCTCAGC CACCTTCTCA GACCTTCCCC CCACCCTCCC TCCCCTCAAA AAGCCTTGCA
    ATGCAGCAGA AGCCCACGGC TTATGTCCGG CCCATGGACG GTCAAGACCA GGCTCCTAGC
    GAGTCTCCTG AACTGAAACC CCTGCCGGAG GAGTACCGGC AGCAGACCTT TGAGAAATCA
    GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACGAAGCTGA AAATGCCTTC TCAGTCAGTC
    GAGCAGACCT ACTCCAATGA AGTCCATTGT GTTGAAGAGA TTCTGAAGGA AATGACCCAC
    TCATGGCCTC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CAGCTGAGCC CTCCAAATTT
    CCTTTTCCCA CAAAGGATTC TCAGCACATC AGTTCTGCGG TCCCAAACCA AAAACAATAT
    GATACATCTT CAAAAACTCA CAGTAATTCT CAGCAGGGAA CATCCATGCT CAAAGATGAC
    CTTCAGCTCA GTGACAGTGA GGACAGTGAC AGCGATCAAA CCTCAGAGAA GCCTCCTTTC
    GCACCTGCAC CTCCAAGCGC TCCACCGGCG CTGCCAGCAG CCGCGGCCTC AGCACATTCC
    AGCAGCGCCG AATCGGAGAG CACCAGCGAC TCCGACAGCT CCTCGGACTC GGAGAGCGAG
    AGCAGCTCCA GTGACAGCGA GGAGAACGAA CCCCGAGAAG CCCCGGCTCC CGAGCCCGAG
    CCTCCGACAA CCAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAGCCCGCG
    GTGCCCACCG AGGCCCCGGG TAGCACGGAG CCCCCGGCCC GACCCCCGGA CACTAAGGGC
    AAGGGCGGCG ACAGCGCCAC CGCGGGTCAC GAGCGCCCAG AGTCCAAAGA GCCTCCCCCC
    AAAGGCTCCA GCAAAGCCCC CCGGGTCCCA TCCGAAGGCC CCCACGCGGG CAAGAGGAGC
    TGTCAGAAGT CCCCTGCCCA GCAGGAGCCC CCCCAGCGGC AAACCGTGGG AAGCAAACAG
    CCGAAAAAGC CCATCAAGGC TTCCGCCCCC GGGCACGCGG ATGCGCCTGC AGATGCGCGT
    GCCTGCCTGC AGGTGGAGAG CGAGCCCGGA CTTCCTCCCC CGGCCTCCAG GGACCAGCCC
    TCCAAAGACA AGCCCAAGGT GAAGACGAAA GGGCGGCCCC GCGCCGCAGA CAGCCGGGAG
    CCCAAGCCGG CGGTGCCCAC CCCCAGCGAC CGGAAGAAGC ACCGCAGCGG CCCCCCGAAG
    GCGCCCCCGA AGGACGCGGC GGCAGAGGAC CGGAGCCCCG AACACTTTGC GCTCGTTCCC
    CTGACCCAGA GCCAGGGCCC TGTCCGCGGT GGCAGCGGCG GCGCCGGCGC CAGGACTAGT
    GGCTGCAGGC TGGCTGTGGT GGTCCAGGAG GACCGCCGCA AAGACAAACC CCCGGTGCCT
    TCGAGAGACA CCAAGCTGCT CTCCCCGCTC AGGGACACTC CGCCGCCACA AAGCTTGGTG
    GTGAAGATCA CACTCGACCT CCTGTCTCGG ATACCCCAGC CCCCAGGGAA GGGCAGCCGC
    CCCAAGAAAC CAGAAGACAA ACAGCCACCA GCAGGGAAGA AGCCAGATCC TGAGAAGAGA
    AGCTCAGAAA ACGCCAGCAA GTTAGCCAAA AAGAGAAAGG GTGAAGCAGA GAAAGACCAC
    GATAGCAAGA AAGTCAGGCT GGAGAAGGAA GTCAAGTCAC AGCCGTTGTC TTCCTCTTCC
    CACAAGGAAT CTTCCAAATC AAAGATGTCC AGGCCCTCCT CTGAGCCCTC AAAGAAGGAA
    ATGCTTCCCC CTTCGCTCGT GTCATCCTCA TCCTCCCAGA AGCCAGCCAA GCCTGCACAG
    AAGAGGCCAC GGCAGGAAGA CCCCGGCTGC CAGGACCCCC AAAAAAGTGC CGGTAGTGCC
    AAGGGCAGCC ACAGAGACCC TTCTGCCTCC AAACACAGAA AAGCAGAGGG AAAGGTCTCT
    GGAAGCTCCA CAGAGCACAA AGGATCTTCT GGAGATACTG CAAACCGTTT TCCAGTGCCT
    TCTTTGCCAA ATGGTAACTC CAAACCAGGG AAGCCTCACG TGAAGTTTGA CAAACAACAA
    GCCGATTTTC ACCTGAAGGA AGCCAAAAGG TTGAAGGACA AAGCAGAATT AGTGACGGAC
    AAGGTAGGGA AGGCCTTTAA ATACCTGGAG GCTGCCTTGT CCTTCATTGA GTGCGGCGTC
    GCCCTGGAGT CGGAGAGCCC CGCGTCCAAG TCGGCGTACT CCATCTACTC GGAGACCGTG
    GACCTCATTA AGTTCATACT GTCATTAAAA TCCTTCTCAG ATGCCACAAC GCCAACACAG
    GAGAAGATAT TTGCTGTTCT ATGCATGCGT TGCCAGTCCA TTTTGAACAT GGCAATGTTT
    CGTTGTAAAA AAGATATGGC GATAAAGTAT TCCCGCACGC TTAACGAACA CTTCTTCAAG
    ACTTCTTCCA AGGTTGCCCA GGCACCTTCT CCATGCATCG CAAGAAGCAC AGGCACACCG
    TCCCCCCTCT CTCCGATCCC TTCTCCGGCC AGCTCCATAG GGTCCCAGTC AAGCGCTGGC
    AGCGTGGGGA GCGGGGCGCT GCCCGCCACC ATCAGCACCC CCGTCACCAT CCAGAACATG
    ACATCCTCCT ACGTCAGCAT CACTTCCCAC GTCCTTACCG CCTTTGACCT GTGGGAACAG
    GCTGAGGTCC TTACCAGGAA GAACAAAGAA TTCTTTGCTC AGCTCAGCAC AAGTGTGTGC
    GCGCTGGCCC TCAACAGCAG TTTGATGGAT CTGGTGCATT ATACAAGACA GGGTTTCCAG
    CGGCTAAAGC AAGTAACCAA GACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20595
    Clone ID Related Accession (Same CDS sequence) XM_010806299.2 , XM_005208211.3
    Accession Version XM_005208211.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103983.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005208211.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CCGAAAGAGT CAACAGCAGC AGCAACAGCA GCTTGTACAA TGAAGACAGA 
    AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGATAAAGAG
    GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCATACA AGACAGAGAA AGGTGATGAG
    CTATCCAGTC GGATACAGAA CATGCTGGGA AACTATGAAG AGATGAAGGA GTTCCTTAGT
    AGCAAGTCCC ACCCCCAGCG CTTGGATGCT TCTGAAAATA GGCTGGGGAA GCCGAGATAC
    CCTTTATTTC CTGAGAAGGG GAGCAGCCTT CCACCCCACT CCTTCCACAC CAGTGTCCAC
    CACCAGCCCG TTAACACTCC TGCCTGTGGG CCACATCCTG CTGGTAACAT CGGCCACCAC
    CCAAAGATGG CGCAGCCAAG AATGGAACCA ATGCCAAGTC TCCACGCCAA AAGCTATGGC
    CCACCGGACA GCCAGCACCT GACCCAAGAG CGCCTCGGTC AGGAGGGGTA CGGCTCTAGT
    CATCACAAAA AGGGTGACCG CAGGGCTGAT GGAGACCACT GTGCTTTGAC GACAGACTCT
    GCTCCGGAGA GGGAACTTTC ACCTCTGTTC TCTTTGCCTT CCCCAGTCCC CCCTTTGTCA
    CCTGTACATT CCAACCAGCA GACTGCTCCC AGGACGCAAG GAAGCGGCAA AGTTCCCAGC
    AGTAGCAGTA GCAATAAAGG CTACTGCCTG GCCAAGTCTC CCAAGGACCT AGCGGTAAAG
    GTGCACGATA AAGAGACCCC TCAAGACAGC ACAGGGGCAG TGGCCAGCCT TGGGGTAGCC
    CCTCCTCAGC CACCTTCTCA GACCTTCCCC CCACCCTCCC TCCCCTCAAA AAGCCTTGCA
    ATGCAGCAGA AGCCCACGGC TTATGTCCGG CCCATGGACG GTCAAGACCA GGCTCCTAGC
    GAGTCTCCTG AACTGAAACC CCTGCCGGAG GAGTACCGGC AGCAGACCTT TGAGAAATCA
    GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACGAAGCTGA AAATGCCTTC TCAGTCAGTC
    GAGCAGACCT ACTCCAATGA AGTCCATTGT GTTGAAGAGA TTCTGAAGGA AATGACCCAC
    TCATGGCCTC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CAGCTGAGCC CTCCAAATTT
    CCTTTTCCCA CAAAGGATTC TCAGCACATC AGTTCTGCGG TCCCAAACCA AAAACAATAT
    GATACATCTT CAAAAACTCA CAGTAATTCT CAGCAGGGAA CATCCATGCT CAAAGATGAC
    CTTCAGCTCA GTGACAGTGA GGACAGTGAC AGCGATCAAA CCTCAGAGAA GCCTCCTTTC
    GCACCTGCAC CTCCAAGCGC TCCACCGGCG CTGCCAGCAG CCGCGGCCTC AGCACATTCC
    AGCAGCGCCG AATCGGAGAG CACCAGCGAC TCCGACAGCT CCTCGGACTC GGAGAGCGAG
    AGCAGCTCCA GTGACAGCGA GGAGAACGAA CCCCGAGAAG CCCCGGCTCC CGAGCCCGAG
    CCTCCGACAA CCAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAGCCCGCG
    GTGCCCACCG AGGCCCCGGG TAGCACGGAG CCCCCGGCCC GACCCCCGGA CACTAAGGGC
    AAGGGCGGCG ACAGCGCCAC CGCGGGTCAC GAGCGCCCAG AGTCCAAAGA GCCTCCCCCC
    AAAGGCTCCA GCAAAGCCCC CCGGGTCCCA TCCGAAGGCC CCCACGCGGG CAAGAGGAGC
    TGTCAGAAGT CCCCTGCCCA GCAGGAGCCC CCCCAGCGGC AAACCGTGGG AAGCAAACAG
    CCGAAAAAGC CCATCAAGGC TTCCGCCCCC GGGCACGCGG ATGCGCCTGC AGATGCGCGT
    GCCTGCCTGC AGGTGGAGAG CGAGCCCGGA CTTCCTCCCC CGGCCTCCAG GGACCAGCCC
    TCCAAAGACA AGCCCAAGGT GAAGACGAAA GGGCGGCCCC GCGCCGCAGA CAGCCGGGAG
    CCCAAGCCGG CGGTGCCCAC CCCCAGCGAC CGGAAGAAGC ACCGCAGCGG CCCCCCGAAG
    GCGCCCCCGA AGGACGCGGC GGCAGAGGAC CGGAGCCCCG AACACTTTGC GCTCGTTCCC
    CTGACCCAGA GCCAGGGCCC TGTCCGCGGT GGCAGCGGCG GCGCCGGCGC CAGGACTAGT
    GGCTGCAGGC TGGCTGTGGT GGTCCAGGAG GACCGCCGCA AAGACAAACC CCCGGTGCCT
    TCGAGAGACA CCAAGCTGCT CTCCCCGCTC AGGGACACTC CGCCGCCACA AAGCTTGGTG
    GTGAAGATCA CACTCGACCT CCTGTCTCGG ATACCCCAGC CCCCAGGGAA GGGCAGCCGC
    CCCAAGAAAC CAGAAGACAA ACAGCCACCA GCAGGGAAGA AGCCAGATCC TGAGAAGAGA
    AGCTCAGAAA ACGCCAGCAA GTTAGCCAAA AAGAGAAAGG GTGAAGCAGA GAAAGACCAC
    GATAGCAAGA AAGTCAGGCT GGAGAAGGAA GTCAAGTCAC AGCCGTTGTC TTCCTCTTCC
    CACAAGGAAT CTTCCAAATC AAAGATGTCC AGGCCCTCCT CTGAGCCCTC AAAGAAGGAA
    ATGCTTCCCC CTTCGCTCGT GTCATCCTCA TCCTCCCAGA AGCCAGCCAA GCCTGCACAG
    AAGAGGCCAC GGCAGGAAGA CCCCGGCTGC CAGGACCCCC AAAAAAGTGC CGGTAGTGCC
    AAGGGCAGCC ACAGAGACCC TTCTGCCTCC AAACACAGAA AAGCAGAGGG AAAGGTCTCT
    GGAAGCTCCA CAGAGCACAA AGGATCTTCT GGAGATACTG CAAACCGTTT TCCAGTGCCT
    TCTTTGCCAA ATGGTAACTC CAAACCAGGG AAGCCTCACG TGAAGTTTGA CAAACAACAA
    GCCGATTTTC ACCTGAAGGA AGCCAAAAGG TTGAAGGACA AAGCAGAATT AGTGACGGAC
    AAGGTAGGGA AGGCCTTTAA ATACCTGGAG GCTGCCTTGT CCTTCATTGA GTGCGGCGTC
    GCCCTGGAGT CGGAGAGCCC CGCGTCCAAG TCGGCGTACT CCATCTACTC GGAGACCGTG
    GACCTCATTA AGTTCATACT GTCATTAAAA TCCTTCTCAG ATGCCACAAC GCCAACACAG
    GAGAAGATAT TTGCTGTTCT ATGCATGCGT TGCCAGTCCA TTTTGAACAT GGCAATGTTT
    CGTTGTAAAA AAGATATGGC GATAAAGTAT TCCCGCACGC TTAACGAACA CTTCTTCAAG
    ACTTCTTCCA AGGTTGCCCA GGCACCTTCT CCATGCATCG CAAGAAGCAC AGGCACACCG
    TCCCCCCTCT CTCCGATCCC TTCTCCGGCC AGCTCCATAG GGTCCCAGTC AAGCGCTGGC
    AGCGTGGGGA GCGGGGCGCT GCCCGCCACC ATCAGCACCC CCGTCACCAT CCAGAACATG
    ACATCCTCCT ACGTCAGCAT CACTTCCCAC GTCCTTACCG CCTTTGACCT GTGGGAACAG
    GCTGAGGTCC TTACCAGGAA GAACAAAGAA TTCTTTGCTC AGCTCAGCAC AAGTGTGTGC
    GCGCTGGCCC TCAACAGCAG TTTGATGGAT CTGGTGCATT ATACAAGACA GGGTTTCCAG
    CGGCTAAAGC AAGTAACCAA GACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208268.1
    CDS233..3922
    Translation

    Target ORF information:

    RefSeq Version XM_005208211.3
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208211.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CCGAAAGAGT CAACAGCAGC AGCAACAGCA GCTTGTACAA TGAAGACAGA 
    AACCTGCTTC GAATTAGAGA GAAGGAAAGA CGCAACCAGG AAGCTCACCA AGATAAAGAG
    GCATTTTCTG AAAAGATCCC CCTTTTTGGA GAGCCATACA AGACAGAGAA AGGTGATGAG
    CTATCCAGTC GGATACAGAA CATGCTGGGA AACTATGAAG AGATGAAGGA GTTCCTTAGT
    AGCAAGTCCC ACCCCCAGCG CTTGGATGCT TCTGAAAATA GGCTGGGGAA GCCGAGATAC
    CCTTTATTTC CTGAGAAGGG GAGCAGCCTT CCACCCCACT CCTTCCACAC CAGTGTCCAC
    CACCAGCCCG TTAACACTCC TGCCTGTGGG CCACATCCTG CTGGTAACAT CGGCCACCAC
    CCAAAGATGG CGCAGCCAAG AATGGAACCA ATGCCAAGTC TCCACGCCAA AAGCTATGGC
    CCACCGGACA GCCAGCACCT GACCCAAGAG CGCCTCGGTC AGGAGGGGTA CGGCTCTAGT
    CATCACAAAA AGGGTGACCG CAGGGCTGAT GGAGACCACT GTGCTTTGAC GACAGACTCT
    GCTCCGGAGA GGGAACTTTC ACCTCTGTTC TCTTTGCCTT CCCCAGTCCC CCCTTTGTCA
    CCTGTACATT CCAACCAGCA GACTGCTCCC AGGACGCAAG GAAGCGGCAA AGTTCCCAGC
    AGTAGCAGTA GCAATAAAGG CTACTGCCTG GCCAAGTCTC CCAAGGACCT AGCGGTAAAG
    GTGCACGATA AAGAGACCCC TCAAGACAGC ACAGGGGCAG TGGCCAGCCT TGGGGTAGCC
    CCTCCTCAGC CACCTTCTCA GACCTTCCCC CCACCCTCCC TCCCCTCAAA AAGCCTTGCA
    ATGCAGCAGA AGCCCACGGC TTATGTCCGG CCCATGGACG GTCAAGACCA GGCTCCTAGC
    GAGTCTCCTG AACTGAAACC CCTGCCGGAG GAGTACCGGC AGCAGACCTT TGAGAAATCA
    GACTTAAAAG TGCCTGCCAA AGCCAAGCTC ACGAAGCTGA AAATGCCTTC TCAGTCAGTC
    GAGCAGACCT ACTCCAATGA AGTCCATTGT GTTGAAGAGA TTCTGAAGGA AATGACCCAC
    TCATGGCCTC CTCCTTTGAC AGCAATACAC ACGCCTAGTA CAGCTGAGCC CTCCAAATTT
    CCTTTTCCCA CAAAGGATTC TCAGCACATC AGTTCTGCGG TCCCAAACCA AAAACAATAT
    GATACATCTT CAAAAACTCA CAGTAATTCT CAGCAGGGAA CATCCATGCT CAAAGATGAC
    CTTCAGCTCA GTGACAGTGA GGACAGTGAC AGCGATCAAA CCTCAGAGAA GCCTCCTTTC
    GCACCTGCAC CTCCAAGCGC TCCACCGGCG CTGCCAGCAG CCGCGGCCTC AGCACATTCC
    AGCAGCGCCG AATCGGAGAG CACCAGCGAC TCCGACAGCT CCTCGGACTC GGAGAGCGAG
    AGCAGCTCCA GTGACAGCGA GGAGAACGAA CCCCGAGAAG CCCCGGCTCC CGAGCCCGAG
    CCTCCGACAA CCAACAAATG GCAGCTGGAC AACTGGCTGA CCAAAGTCAG CCAGCCCGCG
    GTGCCCACCG AGGCCCCGGG TAGCACGGAG CCCCCGGCCC GACCCCCGGA CACTAAGGGC
    AAGGGCGGCG ACAGCGCCAC CGCGGGTCAC GAGCGCCCAG AGTCCAAAGA GCCTCCCCCC
    AAAGGCTCCA GCAAAGCCCC CCGGGTCCCA TCCGAAGGCC CCCACGCGGG CAAGAGGAGC
    TGTCAGAAGT CCCCTGCCCA GCAGGAGCCC CCCCAGCGGC AAACCGTGGG AAGCAAACAG
    CCGAAAAAGC CCATCAAGGC TTCCGCCCCC GGGCACGCGG ATGCGCCTGC AGATGCGCGT
    GCCTGCCTGC AGGTGGAGAG CGAGCCCGGA CTTCCTCCCC CGGCCTCCAG GGACCAGCCC
    TCCAAAGACA AGCCCAAGGT GAAGACGAAA GGGCGGCCCC GCGCCGCAGA CAGCCGGGAG
    CCCAAGCCGG CGGTGCCCAC CCCCAGCGAC CGGAAGAAGC ACCGCAGCGG CCCCCCGAAG
    GCGCCCCCGA AGGACGCGGC GGCAGAGGAC CGGAGCCCCG AACACTTTGC GCTCGTTCCC
    CTGACCCAGA GCCAGGGCCC TGTCCGCGGT GGCAGCGGCG GCGCCGGCGC CAGGACTAGT
    GGCTGCAGGC TGGCTGTGGT GGTCCAGGAG GACCGCCGCA AAGACAAACC CCCGGTGCCT
    TCGAGAGACA CCAAGCTGCT CTCCCCGCTC AGGGACACTC CGCCGCCACA AAGCTTGGTG
    GTGAAGATCA CACTCGACCT CCTGTCTCGG ATACCCCAGC CCCCAGGGAA GGGCAGCCGC
    CCCAAGAAAC CAGAAGACAA ACAGCCACCA GCAGGGAAGA AGCCAGATCC TGAGAAGAGA
    AGCTCAGAAA ACGCCAGCAA GTTAGCCAAA AAGAGAAAGG GTGAAGCAGA GAAAGACCAC
    GATAGCAAGA AAGTCAGGCT GGAGAAGGAA GTCAAGTCAC AGCCGTTGTC TTCCTCTTCC
    CACAAGGAAT CTTCCAAATC AAAGATGTCC AGGCCCTCCT CTGAGCCCTC AAAGAAGGAA
    ATGCTTCCCC CTTCGCTCGT GTCATCCTCA TCCTCCCAGA AGCCAGCCAA GCCTGCACAG
    AAGAGGCCAC GGCAGGAAGA CCCCGGCTGC CAGGACCCCC AAAAAAGTGC CGGTAGTGCC
    AAGGGCAGCC ACAGAGACCC TTCTGCCTCC AAACACAGAA AAGCAGAGGG AAAGGTCTCT
    GGAAGCTCCA CAGAGCACAA AGGATCTTCT GGAGATACTG CAAACCGTTT TCCAGTGCCT
    TCTTTGCCAA ATGGTAACTC CAAACCAGGG AAGCCTCACG TGAAGTTTGA CAAACAACAA
    GCCGATTTTC ACCTGAAGGA AGCCAAAAGG TTGAAGGACA AAGCAGAATT AGTGACGGAC
    AAGGTAGGGA AGGCCTTTAA ATACCTGGAG GCTGCCTTGT CCTTCATTGA GTGCGGCGTC
    GCCCTGGAGT CGGAGAGCCC CGCGTCCAAG TCGGCGTACT CCATCTACTC GGAGACCGTG
    GACCTCATTA AGTTCATACT GTCATTAAAA TCCTTCTCAG ATGCCACAAC GCCAACACAG
    GAGAAGATAT TTGCTGTTCT ATGCATGCGT TGCCAGTCCA TTTTGAACAT GGCAATGTTT
    CGTTGTAAAA AAGATATGGC GATAAAGTAT TCCCGCACGC TTAACGAACA CTTCTTCAAG
    ACTTCTTCCA AGGTTGCCCA GGCACCTTCT CCATGCATCG CAAGAAGCAC AGGCACACCG
    TCCCCCCTCT CTCCGATCCC TTCTCCGGCC AGCTCCATAG GGTCCCAGTC AAGCGCTGGC
    AGCGTGGGGA GCGGGGCGCT GCCCGCCACC ATCAGCACCC CCGTCACCAT CCAGAACATG
    ACATCCTCCT ACGTCAGCAT CACTTCCCAC GTCCTTACCG CCTTTGACCT GTGGGAACAG
    GCTGAGGTCC TTACCAGGAA GAACAAAGAA TTCTTTGCTC AGCTCAGCAC AAGTGTGTGC
    GCGCTGGCCC TCAACAGCAG TTTGATGGAT CTGGTGCATT ATACAAGACA GGGTTTCCAG
    CGGCTAAAGC AAGTAACCAA GACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20597
    Clone ID Related Accession (Same CDS sequence) XM_005208213.3
    Accession Version XM_005208213.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103983.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005208213.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCCC AGTCAAGCTT GTACAATGAA GACAGAAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC TCACCAAGAT AAAGAGGCAT TTTCTGAAAA GATCCCCCTT
    TTTGGAGAGC CATACAAGAC AGAGAAAGGT GATGAGCTAT CCAGTCGGAT ACAGAACATG
    CTGGGAAACT ATGAAGAGAT GAAGGAGTTC CTTAGTAGCA AGTCCCACCC CCAGCGCTTG
    GATGCTTCTG AAAATAGGCT GGGGAAGCCG AGATACCCTT TATTTCCTGA GAAGGGGAGC
    AGCCTTCCAC CCCACTCCTT CCACACCAGT GTCCACCACC AGCCCGTTAA CACTCCTGCC
    TGTGGGCCAC ATCCTGCTGG TAACATCGGC CACCACCCAA AGATGGCGCA GCCAAGAATG
    GAACCAATGC CAAGTCTCCA CGCCAAAAGC TATGGCCCAC CGGACAGCCA GCACCTGACC
    CAAGAGCGCC TCGGTCAGGA GGGGTACGGC TCTAGTCATC ACAAAAAGGG TGACCGCAGG
    GCTGATGGAG ACCACTGTGC TTTGACGACA GACTCTGCTC CGGAGAGGGA ACTTTCACCT
    CTGTTCTCTT TGCCTTCCCC AGTCCCCCCT TTGTCACCTG TACATTCCAA CCAGCAGACT
    GCTCCCAGGA CGCAAGGAAG CGGCAAAGTT CCCAGCAGTA GCAGTAGCAA TAAAGGCTAC
    TGCCTGGCCA AGTCTCCCAA GGACCTAGCG GTAAAGGTGC ACGATAAAGA GACCCCTCAA
    GACAGCACAG GGGCAGTGGC CAGCCTTGGG GTAGCCCCTC CTCAGCCACC TTCTCAGACC
    TTCCCCCCAC CCTCCCTCCC CTCAAAAAGC CTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGACGGTCA AGACCAGGCT CCTAGCGAGT CTCCTGAACT GAAACCCCTG
    CCGGAGGAGT ACCGGCAGCA GACCTTTGAG AAATCAGACT TAAAAGTGCC TGCCAAAGCC
    AAGCTCACGA AGCTGAAAAT GCCTTCTCAG TCAGTCGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCACTCAT GGCCTCCTCC TTTGACAGCA
    ATACACACGC CTAGTACAGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG
    CACATCAGTT CTGCGGTCCC AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACAGT
    AATTCTCAGC AGGGAACATC CATGCTCAAA GATGACCTTC AGCTCAGTGA CAGTGAGGAC
    AGTGACAGCG ATCAAACCTC AGAGAAGCCT CCTTTCGCAC CTGCACCTCC AAGCGCTCCA
    CCGGCGCTGC CAGCAGCCGC GGCCTCAGCA CATTCCAGCA GCGCCGAATC GGAGAGCACC
    AGCGACTCCG ACAGCTCCTC GGACTCGGAG AGCGAGAGCA GCTCCAGTGA CAGCGAGGAG
    AACGAACCCC GAGAAGCCCC GGCTCCCGAG CCCGAGCCTC CGACAACCAA CAAATGGCAG
    CTGGACAACT GGCTGACCAA AGTCAGCCAG CCCGCGGTGC CCACCGAGGC CCCGGGTAGC
    ACGGAGCCCC CGGCCCGACC CCCGGACACT AAGGGCAAGG GCGGCGACAG CGCCACCGCG
    GGTCACGAGC GCCCAGAGTC CAAAGAGCCT CCCCCCAAAG GCTCCAGCAA AGCCCCCCGG
    GTCCCATCCG AAGGCCCCCA CGCGGGCAAG AGGAGCTGTC AGAAGTCCCC TGCCCAGCAG
    GAGCCCCCCC AGCGGCAAAC CGTGGGAAGC AAACAGCCGA AAAAGCCCAT CAAGGCTTCC
    GCCCCCGGGC ACGCGGATGC GCCTGCAGAT GCGCGTGCCT GCCTGCAGGT GGAGAGCGAG
    CCCGGACTTC CTCCCCCGGC CTCCAGGGAC CAGCCCTCCA AAGACAAGCC CAAGGTGAAG
    ACGAAAGGGC GGCCCCGCGC CGCAGACAGC CGGGAGCCCA AGCCGGCGGT GCCCACCCCC
    AGCGACCGGA AGAAGCACCG CAGCGGCCCC CCGAAGGCGC CCCCGAAGGA CGCGGCGGCA
    GAGGACCGGA GCCCCGAACA CTTTGCGCTC GTTCCCCTGA CCCAGAGCCA GGGCCCTGTC
    CGCGGTGGCA GCGGCGGCGC CGGCGCCAGG ACTAGTGGCT GCAGGCTGGC TGTGGTGGTC
    CAGGAGGACC GCCGCAAAGA CAAACCCCCG GTGCCTTCGA GAGACACCAA GCTGCTCTCC
    CCGCTCAGGG ACACTCCGCC GCCACAAAGC TTGGTGGTGA AGATCACACT CGACCTCCTG
    TCTCGGATAC CCCAGCCCCC AGGGAAGGGC AGCCGCCCCA AGAAACCAGA AGACAAACAG
    CCACCAGCAG GGAAGAAGCC AGATCCTGAG AAGAGAAGCT CAGAAAACGC CAGCAAGTTA
    GCCAAAAAGA GAAAGGGTGA AGCAGAGAAA GACCACGATA GCAAGAAAGT CAGGCTGGAG
    AAGGAAGTCA AGTCACAGCC GTTGTCTTCC TCTTCCCACA AGGAATCTTC CAAATCAAAG
    ATGTCCAGGC CCTCCTCTGA GCCCTCAAAG AAGGAAATGC TTCCCCCTTC GCTCGTGTCA
    TCCTCATCCT CCCAGAAGCC AGCCAAGCCT GCACAGAAGA GGCCACGGCA GGAAGACCCC
    GGCTGCCAGG ACCCCCAAAA AAGTGCCGGT AGTGCCAAGG GCAGCCACAG AGACCCTTCT
    GCCTCCAAAC ACAGAAAAGC AGAGGGAAAG GTCTCTGGAA GCTCCACAGA GCACAAAGGA
    TCTTCTGGAG ATACTGCAAA CCGTTTTCCA GTGCCTTCTT TGCCAAATGG TAACTCCAAA
    CCAGGGAAGC CTCACGTGAA GTTTGACAAA CAACAAGCCG ATTTTCACCT GAAGGAAGCC
    AAAAGGTTGA AGGACAAAGC AGAATTAGTG ACGGACAAGG TAGGGAAGGC CTTTAAATAC
    CTGGAGGCTG CCTTGTCCTT CATTGAGTGC GGCGTCGCCC TGGAGTCGGA GAGCCCCGCG
    TCCAAGTCGG CGTACTCCAT CTACTCGGAG ACCGTGGACC TCATTAAGTT CATACTGTCA
    TTAAAATCCT TCTCAGATGC CACAACGCCA ACACAGGAGA AGATATTTGC TGTTCTATGC
    ATGCGTTGCC AGTCCATTTT GAACATGGCA ATGTTTCGTT GTAAAAAAGA TATGGCGATA
    AAGTATTCCC GCACGCTTAA CGAACACTTC TTCAAGACTT CTTCCAAGGT TGCCCAGGCA
    CCTTCTCCAT GCATCGCAAG AAGCACAGGC ACACCGTCCC CCCTCTCTCC GATCCCTTCT
    CCGGCCAGCT CCATAGGGTC CCAGTCAAGC GCTGGCAGCG TGGGGAGCGG GGCGCTGCCC
    GCCACCATCA GCACCCCCGT CACCATCCAG AACATGACAT CCTCCTACGT CAGCATCACT
    TCCCACGTCC TTACCGCCTT TGACCTGTGG GAACAGGCTG AGGTCCTTAC CAGGAAGAAC
    AAAGAATTCT TTGCTCAGCT CAGCACAAGT GTGTGCGCGC TGGCCCTCAA CAGCAGTTTG
    ATGGATCTGG TGCATTATAC AAGACAGGGT TTCCAGCGGC TAAAGCAAGT AACCAAGACA
    CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208270.1
    CDS176..3841
    Translation

    Target ORF information:

    RefSeq Version XM_005208213.3
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208213.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCCC AGTCAAGCTT GTACAATGAA GACAGAAACC TGCTTCGAAT TAGAGAGAAG 
    GAAAGACGCA ACCAGGAAGC TCACCAAGAT AAAGAGGCAT TTTCTGAAAA GATCCCCCTT
    TTTGGAGAGC CATACAAGAC AGAGAAAGGT GATGAGCTAT CCAGTCGGAT ACAGAACATG
    CTGGGAAACT ATGAAGAGAT GAAGGAGTTC CTTAGTAGCA AGTCCCACCC CCAGCGCTTG
    GATGCTTCTG AAAATAGGCT GGGGAAGCCG AGATACCCTT TATTTCCTGA GAAGGGGAGC
    AGCCTTCCAC CCCACTCCTT CCACACCAGT GTCCACCACC AGCCCGTTAA CACTCCTGCC
    TGTGGGCCAC ATCCTGCTGG TAACATCGGC CACCACCCAA AGATGGCGCA GCCAAGAATG
    GAACCAATGC CAAGTCTCCA CGCCAAAAGC TATGGCCCAC CGGACAGCCA GCACCTGACC
    CAAGAGCGCC TCGGTCAGGA GGGGTACGGC TCTAGTCATC ACAAAAAGGG TGACCGCAGG
    GCTGATGGAG ACCACTGTGC TTTGACGACA GACTCTGCTC CGGAGAGGGA ACTTTCACCT
    CTGTTCTCTT TGCCTTCCCC AGTCCCCCCT TTGTCACCTG TACATTCCAA CCAGCAGACT
    GCTCCCAGGA CGCAAGGAAG CGGCAAAGTT CCCAGCAGTA GCAGTAGCAA TAAAGGCTAC
    TGCCTGGCCA AGTCTCCCAA GGACCTAGCG GTAAAGGTGC ACGATAAAGA GACCCCTCAA
    GACAGCACAG GGGCAGTGGC CAGCCTTGGG GTAGCCCCTC CTCAGCCACC TTCTCAGACC
    TTCCCCCCAC CCTCCCTCCC CTCAAAAAGC CTTGCAATGC AGCAGAAGCC CACGGCTTAT
    GTCCGGCCCA TGGACGGTCA AGACCAGGCT CCTAGCGAGT CTCCTGAACT GAAACCCCTG
    CCGGAGGAGT ACCGGCAGCA GACCTTTGAG AAATCAGACT TAAAAGTGCC TGCCAAAGCC
    AAGCTCACGA AGCTGAAAAT GCCTTCTCAG TCAGTCGAGC AGACCTACTC CAATGAAGTC
    CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCACTCAT GGCCTCCTCC TTTGACAGCA
    ATACACACGC CTAGTACAGC TGAGCCCTCC AAATTTCCTT TTCCCACAAA GGATTCTCAG
    CACATCAGTT CTGCGGTCCC AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACAGT
    AATTCTCAGC AGGGAACATC CATGCTCAAA GATGACCTTC AGCTCAGTGA CAGTGAGGAC
    AGTGACAGCG ATCAAACCTC AGAGAAGCCT CCTTTCGCAC CTGCACCTCC AAGCGCTCCA
    CCGGCGCTGC CAGCAGCCGC GGCCTCAGCA CATTCCAGCA GCGCCGAATC GGAGAGCACC
    AGCGACTCCG ACAGCTCCTC GGACTCGGAG AGCGAGAGCA GCTCCAGTGA CAGCGAGGAG
    AACGAACCCC GAGAAGCCCC GGCTCCCGAG CCCGAGCCTC CGACAACCAA CAAATGGCAG
    CTGGACAACT GGCTGACCAA AGTCAGCCAG CCCGCGGTGC CCACCGAGGC CCCGGGTAGC
    ACGGAGCCCC CGGCCCGACC CCCGGACACT AAGGGCAAGG GCGGCGACAG CGCCACCGCG
    GGTCACGAGC GCCCAGAGTC CAAAGAGCCT CCCCCCAAAG GCTCCAGCAA AGCCCCCCGG
    GTCCCATCCG AAGGCCCCCA CGCGGGCAAG AGGAGCTGTC AGAAGTCCCC TGCCCAGCAG
    GAGCCCCCCC AGCGGCAAAC CGTGGGAAGC AAACAGCCGA AAAAGCCCAT CAAGGCTTCC
    GCCCCCGGGC ACGCGGATGC GCCTGCAGAT GCGCGTGCCT GCCTGCAGGT GGAGAGCGAG
    CCCGGACTTC CTCCCCCGGC CTCCAGGGAC CAGCCCTCCA AAGACAAGCC CAAGGTGAAG
    ACGAAAGGGC GGCCCCGCGC CGCAGACAGC CGGGAGCCCA AGCCGGCGGT GCCCACCCCC
    AGCGACCGGA AGAAGCACCG CAGCGGCCCC CCGAAGGCGC CCCCGAAGGA CGCGGCGGCA
    GAGGACCGGA GCCCCGAACA CTTTGCGCTC GTTCCCCTGA CCCAGAGCCA GGGCCCTGTC
    CGCGGTGGCA GCGGCGGCGC CGGCGCCAGG ACTAGTGGCT GCAGGCTGGC TGTGGTGGTC
    CAGGAGGACC GCCGCAAAGA CAAACCCCCG GTGCCTTCGA GAGACACCAA GCTGCTCTCC
    CCGCTCAGGG ACACTCCGCC GCCACAAAGC TTGGTGGTGA AGATCACACT CGACCTCCTG
    TCTCGGATAC CCCAGCCCCC AGGGAAGGGC AGCCGCCCCA AGAAACCAGA AGACAAACAG
    CCACCAGCAG GGAAGAAGCC AGATCCTGAG AAGAGAAGCT CAGAAAACGC CAGCAAGTTA
    GCCAAAAAGA GAAAGGGTGA AGCAGAGAAA GACCACGATA GCAAGAAAGT CAGGCTGGAG
    AAGGAAGTCA AGTCACAGCC GTTGTCTTCC TCTTCCCACA AGGAATCTTC CAAATCAAAG
    ATGTCCAGGC CCTCCTCTGA GCCCTCAAAG AAGGAAATGC TTCCCCCTTC GCTCGTGTCA
    TCCTCATCCT CCCAGAAGCC AGCCAAGCCT GCACAGAAGA GGCCACGGCA GGAAGACCCC
    GGCTGCCAGG ACCCCCAAAA AAGTGCCGGT AGTGCCAAGG GCAGCCACAG AGACCCTTCT
    GCCTCCAAAC ACAGAAAAGC AGAGGGAAAG GTCTCTGGAA GCTCCACAGA GCACAAAGGA
    TCTTCTGGAG ATACTGCAAA CCGTTTTCCA GTGCCTTCTT TGCCAAATGG TAACTCCAAA
    CCAGGGAAGC CTCACGTGAA GTTTGACAAA CAACAAGCCG ATTTTCACCT GAAGGAAGCC
    AAAAGGTTGA AGGACAAAGC AGAATTAGTG ACGGACAAGG TAGGGAAGGC CTTTAAATAC
    CTGGAGGCTG CCTTGTCCTT CATTGAGTGC GGCGTCGCCC TGGAGTCGGA GAGCCCCGCG
    TCCAAGTCGG CGTACTCCAT CTACTCGGAG ACCGTGGACC TCATTAAGTT CATACTGTCA
    TTAAAATCCT TCTCAGATGC CACAACGCCA ACACAGGAGA AGATATTTGC TGTTCTATGC
    ATGCGTTGCC AGTCCATTTT GAACATGGCA ATGTTTCGTT GTAAAAAAGA TATGGCGATA
    AAGTATTCCC GCACGCTTAA CGAACACTTC TTCAAGACTT CTTCCAAGGT TGCCCAGGCA
    CCTTCTCCAT GCATCGCAAG AAGCACAGGC ACACCGTCCC CCCTCTCTCC GATCCCTTCT
    CCGGCCAGCT CCATAGGGTC CCAGTCAAGC GCTGGCAGCG TGGGGAGCGG GGCGCTGCCC
    GCCACCATCA GCACCCCCGT CACCATCCAG AACATGACAT CCTCCTACGT CAGCATCACT
    TCCCACGTCC TTACCGCCTT TGACCTGTGG GAACAGGCTG AGGTCCTTAC CAGGAAGAAC
    AAAGAATTCT TTGCTCAGCT CAGCACAAGT GTGTGCGCGC TGGCCCTCAA CAGCAGTTTG
    ATGGATCTGG TGCATTATAC AAGACAGGGT TTCCAGCGGC TAAAGCAAGT AACCAAGACA
    CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57021
    Clone ID Related Accession (Same CDS sequence) XM_010806300.2
    Accession Version XM_010806300.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3687bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103983.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806300.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CCGAAAGAGT CAACAGCAGC AGCAACAGCT TGTACAATGA AGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CTCACCAAGA TAAAGAGGCA
    TTTTCTGAAA AGATCCCCCT TTTTGGAGAG CCATACAAGA CAGAGAAAGG TGATGAGCTA
    TCCAGTCGGA TACAGAACAT GCTGGGAAAC TATGAAGAGA TGAAGGAGTT CCTTAGTAGC
    AAGTCCCACC CCCAGCGCTT GGATGCTTCT GAAAATAGGC TGGGGAAGCC GAGATACCCT
    TTATTTCCTG AGAAGGGGAG CAGCCTTCCA CCCCACTCCT TCCACACCAG TGTCCACCAC
    CAGCCCGTTA ACACTCCTGC CTGTGGGCCA CATCCTGCTG GTAACATCGG CCACCACCCA
    AAGATGGCGC AGCCAAGAAT GGAACCAATG CCAAGTCTCC ACGCCAAAAG CTATGGCCCA
    CCGGACAGCC AGCACCTGAC CCAAGAGCGC CTCGGTCAGG AGGGGTACGG CTCTAGTCAT
    CACAAAAAGG GTGACCGCAG GGCTGATGGA GACCACTGTG CTTTGACGAC AGACTCTGCT
    CCGGAGAGGG AACTTTCACC TCTGTTCTCT TTGCCTTCCC CAGTCCCCCC TTTGTCACCT
    GTACATTCCA ACCAGCAGAC TGCTCCCAGG ACGCAAGGAA GCGGCAAAGT TCCCAGCAGT
    AGCAGTAGCA ATAAAGGCTA CTGCCTGGCC AAGTCTCCCA AGGACCTAGC GGTAAAGGTG
    CACGATAAAG AGACCCCTCA AGACAGCACA GGGGCAGTGG CCAGCCTTGG GGTAGCCCCT
    CCTCAGCCAC CTTCTCAGAC CTTCCCCCCA CCCTCCCTCC CCTCAAAAAG CCTTGCAATG
    CAGCAGAAGC CCACGGCTTA TGTCCGGCCC ATGGACGGTC AAGACCAGGC TCCTAGCGAG
    TCTCCTGAAC TGAAACCCCT GCCGGAGGAG TACCGGCAGC AGACCTTTGA GAAATCAGAC
    TTAAAAGTGC CTGCCAAAGC CAAGCTCACG AAGCTGAAAA TGCCTTCTCA GTCAGTCGAG
    CAGACCTACT CCAATGAAGT CCATTGTGTT GAAGAGATTC TGAAGGAAAT GACCCACTCA
    TGGCCTCCTC CTTTGACAGC AATACACACG CCTAGTACAG CTGAGCCCTC CAAATTTCCT
    TTTCCCACAA AGGATTCTCA GCACATCAGT TCTGCGGTCC CAAACCAAAA ACAATATGAT
    ACATCTTCAA AAACTCACAG TAATTCTCAG CAGGGAACAT CCATGCTCAA AGATGACCTT
    CAGCTCAGTG ACAGTGAGGA CAGTGACAGC GATCAAACCT CAGAGAAGCC TCCTTTCGCA
    CCTGCACCTC CAAGCGCTCC ACCGGCGCTG CCAGCAGCCG CGGCCTCAGC ACATTCCAGC
    AGCGCCGAAT CGGAGAGCAC CAGCGACTCC GACAGCTCCT CGGACTCGGA GAGCGAGAGC
    AGCTCCAGTG ACAGCGAGGA GAACGAACCC CGAGAAGCCC CGGCTCCCGA GCCCGAGCCT
    CCGACAACCA ACAAATGGCA GCTGGACAAC TGGCTGACCA AAGTCAGCCA GCCCGCGGTG
    CCCACCGAGG CCCCGGGTAG CACGGAGCCC CCGGCCCGAC CCCCGGACAC TAAGGGCAAG
    GGCGGCGACA GCGCCACCGC GGGTCACGAG CGCCCAGAGT CCAAAGAGCC TCCCCCCAAA
    GGCTCCAGCA AAGCCCCCCG GGTCCCATCC GAAGGCCCCC ACGCGGGCAA GAGGAGCTGT
    CAGAAGTCCC CTGCCCAGCA GGAGCCCCCC CAGCGGCAAA CCGTGGGAAG CAAACAGCCG
    AAAAAGCCCA TCAAGGCTTC CGCCCCCGGG CACGCGGATG CGCCTGCAGA TGCGCGTGCC
    TGCCTGCAGG TGGAGAGCGA GCCCGGACTT CCTCCCCCGG CCTCCAGGGA CCAGCCCTCC
    AAAGACAAGC CCAAGGTGAA GACGAAAGGG CGGCCCCGCG CCGCAGACAG CCGGGAGCCC
    AAGCCGGCGG TGCCCACCCC CAGCGACCGG AAGAAGCACC GCAGCGGCCC CCCGAAGGCG
    CCCCCGAAGG ACGCGGCGGC AGAGGACCGG AGCCCCGAAC ACTTTGCGCT CGTTCCCCTG
    ACCCAGAGCC AGGGCCCTGT CCGCGGTGGC AGCGGCGGCG CCGGCGCCAG GACTAGTGGC
    TGCAGGCTGG CTGTGGTGGT CCAGGAGGAC CGCCGCAAAG ACAAACCCCC GGTGCCTTCG
    AGAGACACCA AGCTGCTCTC CCCGCTCAGG GACACTCCGC CGCCACAAAG CTTGGTGGTG
    AAGATCACAC TCGACCTCCT GTCTCGGATA CCCCAGCCCC CAGGGAAGGG CAGCCGCCCC
    AAGAAACCAG AAGACAAACA GCCACCAGCA GGGAAGAAGC CAGATCCTGA GAAGAGAAGC
    TCAGAAAACG CCAGCAAGTT AGCCAAAAAG AGAAAGGGTG AAGCAGAGAA AGACCACGAT
    AGCAAGAAAG TCAGGCTGGA GAAGGAAGTC AAGTCACAGC CGTTGTCTTC CTCTTCCCAC
    AAGGAATCTT CCAAATCAAA GATGTCCAGG CCCTCCTCTG AGCCCTCAAA GAAGGAAATG
    CTTCCCCCTT CGCTCGTGTC ATCCTCATCC TCCCAGAAGC CAGCCAAGCC TGCACAGAAG
    AGGCCACGGC AGGAAGACCC CGGCTGCCAG GACCCCCAAA AAAGTGCCGG TAGTGCCAAG
    GGCAGCCACA GAGACCCTTC TGCCTCCAAA CACAGAAAAG CAGAGGGAAA GGTCTCTGGA
    AGCTCCACAG AGCACAAAGG ATCTTCTGGA GATACTGCAA ACCGTTTTCC AGTGCCTTCT
    TTGCCAAATG GTAACTCCAA ACCAGGGAAG CCTCACGTGA AGTTTGACAA ACAACAAGCC
    GATTTTCACC TGAAGGAAGC CAAAAGGTTG AAGGACAAAG CAGAATTAGT GACGGACAAG
    GTAGGGAAGG CCTTTAAATA CCTGGAGGCT GCCTTGTCCT TCATTGAGTG CGGCGTCGCC
    CTGGAGTCGG AGAGCCCCGC GTCCAAGTCG GCGTACTCCA TCTACTCGGA GACCGTGGAC
    CTCATTAAGT TCATACTGTC ATTAAAATCC TTCTCAGATG CCACAACGCC AACACAGGAG
    AAGATATTTG CTGTTCTATG CATGCGTTGC CAGTCCATTT TGAACATGGC AATGTTTCGT
    TGTAAAAAAG ATATGGCGAT AAAGTATTCC CGCACGCTTA ACGAACACTT CTTCAAGACT
    TCTTCCAAGG TTGCCCAGGC ACCTTCTCCA TGCATCGCAA GAAGCACAGG CACACCGTCC
    CCCCTCTCTC CGATCCCTTC TCCGGCCAGC TCCATAGGGT CCCAGTCAAG CGCTGGCAGC
    GTGGGGAGCG GGGCGCTGCC CGCCACCATC AGCACCCCCG TCACCATCCA GAACATGACA
    TCCTCCTACG TCAGCATCAC TTCCCACGTC CTTACCGCCT TTGACCTGTG GGAACAGGCT
    GAGGTCCTTA CCAGGAAGAA CAAAGAATTC TTTGCTCAGC TCAGCACAAG TGTGTGCGCG
    CTGGCCCTCA ACAGCAGTTT GATGGATCTG GTGCATTATA CAAGACAGGG TTTCCAGCGG
    CTAAAGCAAG TAACCAAGAC ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804602.1
    CDS233..3919
    Translation

    Target ORF information:

    RefSeq Version XM_010806300.2
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806300.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTTTA CCGAAAGAGT CAACAGCAGC AGCAACAGCT TGTACAATGA AGACAGAAAC 
    CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CTCACCAAGA TAAAGAGGCA
    TTTTCTGAAA AGATCCCCCT TTTTGGAGAG CCATACAAGA CAGAGAAAGG TGATGAGCTA
    TCCAGTCGGA TACAGAACAT GCTGGGAAAC TATGAAGAGA TGAAGGAGTT CCTTAGTAGC
    AAGTCCCACC CCCAGCGCTT GGATGCTTCT GAAAATAGGC TGGGGAAGCC GAGATACCCT
    TTATTTCCTG AGAAGGGGAG CAGCCTTCCA CCCCACTCCT TCCACACCAG TGTCCACCAC
    CAGCCCGTTA ACACTCCTGC CTGTGGGCCA CATCCTGCTG GTAACATCGG CCACCACCCA
    AAGATGGCGC AGCCAAGAAT GGAACCAATG CCAAGTCTCC ACGCCAAAAG CTATGGCCCA
    CCGGACAGCC AGCACCTGAC CCAAGAGCGC CTCGGTCAGG AGGGGTACGG CTCTAGTCAT
    CACAAAAAGG GTGACCGCAG GGCTGATGGA GACCACTGTG CTTTGACGAC AGACTCTGCT
    CCGGAGAGGG AACTTTCACC TCTGTTCTCT TTGCCTTCCC CAGTCCCCCC TTTGTCACCT
    GTACATTCCA ACCAGCAGAC TGCTCCCAGG ACGCAAGGAA GCGGCAAAGT TCCCAGCAGT
    AGCAGTAGCA ATAAAGGCTA CTGCCTGGCC AAGTCTCCCA AGGACCTAGC GGTAAAGGTG
    CACGATAAAG AGACCCCTCA AGACAGCACA GGGGCAGTGG CCAGCCTTGG GGTAGCCCCT
    CCTCAGCCAC CTTCTCAGAC CTTCCCCCCA CCCTCCCTCC CCTCAAAAAG CCTTGCAATG
    CAGCAGAAGC CCACGGCTTA TGTCCGGCCC ATGGACGGTC AAGACCAGGC TCCTAGCGAG
    TCTCCTGAAC TGAAACCCCT GCCGGAGGAG TACCGGCAGC AGACCTTTGA GAAATCAGAC
    TTAAAAGTGC CTGCCAAAGC CAAGCTCACG AAGCTGAAAA TGCCTTCTCA GTCAGTCGAG
    CAGACCTACT CCAATGAAGT CCATTGTGTT GAAGAGATTC TGAAGGAAAT GACCCACTCA
    TGGCCTCCTC CTTTGACAGC AATACACACG CCTAGTACAG CTGAGCCCTC CAAATTTCCT
    TTTCCCACAA AGGATTCTCA GCACATCAGT TCTGCGGTCC CAAACCAAAA ACAATATGAT
    ACATCTTCAA AAACTCACAG TAATTCTCAG CAGGGAACAT CCATGCTCAA AGATGACCTT
    CAGCTCAGTG ACAGTGAGGA CAGTGACAGC GATCAAACCT CAGAGAAGCC TCCTTTCGCA
    CCTGCACCTC CAAGCGCTCC ACCGGCGCTG CCAGCAGCCG CGGCCTCAGC ACATTCCAGC
    AGCGCCGAAT CGGAGAGCAC CAGCGACTCC GACAGCTCCT CGGACTCGGA GAGCGAGAGC
    AGCTCCAGTG ACAGCGAGGA GAACGAACCC CGAGAAGCCC CGGCTCCCGA GCCCGAGCCT
    CCGACAACCA ACAAATGGCA GCTGGACAAC TGGCTGACCA AAGTCAGCCA GCCCGCGGTG
    CCCACCGAGG CCCCGGGTAG CACGGAGCCC CCGGCCCGAC CCCCGGACAC TAAGGGCAAG
    GGCGGCGACA GCGCCACCGC GGGTCACGAG CGCCCAGAGT CCAAAGAGCC TCCCCCCAAA
    GGCTCCAGCA AAGCCCCCCG GGTCCCATCC GAAGGCCCCC ACGCGGGCAA GAGGAGCTGT
    CAGAAGTCCC CTGCCCAGCA GGAGCCCCCC CAGCGGCAAA CCGTGGGAAG CAAACAGCCG
    AAAAAGCCCA TCAAGGCTTC CGCCCCCGGG CACGCGGATG CGCCTGCAGA TGCGCGTGCC
    TGCCTGCAGG TGGAGAGCGA GCCCGGACTT CCTCCCCCGG CCTCCAGGGA CCAGCCCTCC
    AAAGACAAGC CCAAGGTGAA GACGAAAGGG CGGCCCCGCG CCGCAGACAG CCGGGAGCCC
    AAGCCGGCGG TGCCCACCCC CAGCGACCGG AAGAAGCACC GCAGCGGCCC CCCGAAGGCG
    CCCCCGAAGG ACGCGGCGGC AGAGGACCGG AGCCCCGAAC ACTTTGCGCT CGTTCCCCTG
    ACCCAGAGCC AGGGCCCTGT CCGCGGTGGC AGCGGCGGCG CCGGCGCCAG GACTAGTGGC
    TGCAGGCTGG CTGTGGTGGT CCAGGAGGAC CGCCGCAAAG ACAAACCCCC GGTGCCTTCG
    AGAGACACCA AGCTGCTCTC CCCGCTCAGG GACACTCCGC CGCCACAAAG CTTGGTGGTG
    AAGATCACAC TCGACCTCCT GTCTCGGATA CCCCAGCCCC CAGGGAAGGG CAGCCGCCCC
    AAGAAACCAG AAGACAAACA GCCACCAGCA GGGAAGAAGC CAGATCCTGA GAAGAGAAGC
    TCAGAAAACG CCAGCAAGTT AGCCAAAAAG AGAAAGGGTG AAGCAGAGAA AGACCACGAT
    AGCAAGAAAG TCAGGCTGGA GAAGGAAGTC AAGTCACAGC CGTTGTCTTC CTCTTCCCAC
    AAGGAATCTT CCAAATCAAA GATGTCCAGG CCCTCCTCTG AGCCCTCAAA GAAGGAAATG
    CTTCCCCCTT CGCTCGTGTC ATCCTCATCC TCCCAGAAGC CAGCCAAGCC TGCACAGAAG
    AGGCCACGGC AGGAAGACCC CGGCTGCCAG GACCCCCAAA AAAGTGCCGG TAGTGCCAAG
    GGCAGCCACA GAGACCCTTC TGCCTCCAAA CACAGAAAAG CAGAGGGAAA GGTCTCTGGA
    AGCTCCACAG AGCACAAAGG ATCTTCTGGA GATACTGCAA ACCGTTTTCC AGTGCCTTCT
    TTGCCAAATG GTAACTCCAA ACCAGGGAAG CCTCACGTGA AGTTTGACAA ACAACAAGCC
    GATTTTCACC TGAAGGAAGC CAAAAGGTTG AAGGACAAAG CAGAATTAGT GACGGACAAG
    GTAGGGAAGG CCTTTAAATA CCTGGAGGCT GCCTTGTCCT TCATTGAGTG CGGCGTCGCC
    CTGGAGTCGG AGAGCCCCGC GTCCAAGTCG GCGTACTCCA TCTACTCGGA GACCGTGGAC
    CTCATTAAGT TCATACTGTC ATTAAAATCC TTCTCAGATG CCACAACGCC AACACAGGAG
    AAGATATTTG CTGTTCTATG CATGCGTTGC CAGTCCATTT TGAACATGGC AATGTTTCGT
    TGTAAAAAAG ATATGGCGAT AAAGTATTCC CGCACGCTTA ACGAACACTT CTTCAAGACT
    TCTTCCAAGG TTGCCCAGGC ACCTTCTCCA TGCATCGCAA GAAGCACAGG CACACCGTCC
    CCCCTCTCTC CGATCCCTTC TCCGGCCAGC TCCATAGGGT CCCAGTCAAG CGCTGGCAGC
    GTGGGGAGCG GGGCGCTGCC CGCCACCATC AGCACCCCCG TCACCATCCA GAACATGACA
    TCCTCCTACG TCAGCATCAC TTCCCACGTC CTTACCGCCT TTGACCTGTG GGAACAGGCT
    GAGGTCCTTA CCAGGAAGAA CAAAGAATTC TTTGCTCAGC TCAGCACAAG TGTGTGCGCG
    CTGGCCCTCA ACAGCAGTTT GATGGATCTG GTGCATTATA CAAGACAGGG TTTCCAGCGG
    CTAAAGCAAG TAACCAAGAC ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57022
    Clone ID Related Accession (Same CDS sequence) XM_010806302.2
    Accession Version XM_010806302.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103983.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806302.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCCC AGTCAAGCAG CTTGTACAAT GAAGACAGAA ACCTGCTTCG AATTAGAGAG 
    AAGGAAAGAC GCAACCAGGA AGCTCACCAA GATAAAGAGG CATTTTCTGA AAAGATCCCC
    CTTTTTGGAG AGCCATACAA GACAGAGAAA GGTGATGAGC TATCCAGTCG GATACAGAAC
    ATGCTGGGAA ACTATGAAGA GATGAAGGAG TTCCTTAGTA GCAAGTCCCA CCCCCAGCGC
    TTGGATGCTT CTGAAAATAG GCTGGGGAAG CCGAGATACC CTTTATTTCC TGAGAAGGGG
    AGCAGCCTTC CACCCCACTC CTTCCACACC AGTGTCCACC ACCAGCCCGT TAACACTCCT
    GCCTGTGGGC CACATCCTGC TGGTAACATC GGCCACCACC CAAAGATGGC GCAGCCAAGA
    ATGGAACCAA TGCCAAGTCT CCACGCCAAA AGCTATGGCC CACCGGACAG CCAGCACCTG
    ACCCAAGAGC GCCTCGGTCA GGAGGGGTAC GGCTCTAGTC ATCACAAAAA GGGTGACCGC
    AGGGCTGATG GAGACCACTG TGCTTTGACG ACAGACTCTG CTCCGGAGAG GGAACTTTCA
    CCTCTGTTCT CTTTGCCTTC CCCAGTCCCC CCTTTGTCAC CTGTACATTC CAACCAGCAG
    ACTGCTCCCA GGACGCAAGG AAGCGGCAAA GTTCCCAGCA GTAGCAGTAG CAATAAAGGC
    TACTGCCTGG CCAAGTCTCC CAAGGACCTA GCGGTAAAGG TGCACGATAA AGAGACCCCT
    CAAGACAGCA CAGGGGCAGT GGCCAGCCTT GGGGTAGCCC CTCCTCAGCC ACCTTCTCAG
    ACCTTCCCCC CACCCTCCCT CCCCTCAAAA AGCCTTGCAA TGCAGCAGAA GCCCACGGCT
    TATGTCCGGC CCATGGACGG TCAAGACCAG GCTCCTAGCG AGTCTCCTGA ACTGAAACCC
    CTGCCGGAGG AGTACCGGCA GCAGACCTTT GAGAAATCAG ACTTAAAAGT GCCTGCCAAA
    GCCAAGCTCA CGAAGCTGAA AATGCCTTCT CAGTCAGTCG AGCAGACCTA CTCCAATGAA
    GTCCATTGTG TTGAAGAGAT TCTGAAGGAA ATGACCCACT CATGGCCTCC TCCTTTGACA
    GCAATACACA CGCCTAGTAC AGCTGAGCCC TCCAAATTTC CTTTTCCCAC AAAGGATTCT
    CAGCACATCA GTTCTGCGGT CCCAAACCAA AAACAATATG ATACATCTTC AAAAACTCAC
    AGTAATTCTC AGCAGGGAAC ATCCATGCTC AAAGATGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GCGATCAAAC CTCAGAGAAG CCTCCTTTCG CACCTGCACC TCCAAGCGCT
    CCACCGGCGC TGCCAGCAGC CGCGGCCTCA GCACATTCCA GCAGCGCCGA ATCGGAGAGC
    ACCAGCGACT CCGACAGCTC CTCGGACTCG GAGAGCGAGA GCAGCTCCAG TGACAGCGAG
    GAGAACGAAC CCCGAGAAGC CCCGGCTCCC GAGCCCGAGC CTCCGACAAC CAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCCGCGG TGCCCACCGA GGCCCCGGGT
    AGCACGGAGC CCCCGGCCCG ACCCCCGGAC ACTAAGGGCA AGGGCGGCGA CAGCGCCACC
    GCGGGTCACG AGCGCCCAGA GTCCAAAGAG CCTCCCCCCA AAGGCTCCAG CAAAGCCCCC
    CGGGTCCCAT CCGAAGGCCC CCACGCGGGC AAGAGGAGCT GTCAGAAGTC CCCTGCCCAG
    CAGGAGCCCC CCCAGCGGCA AACCGTGGGA AGCAAACAGC CGAAAAAGCC CATCAAGGCT
    TCCGCCCCCG GGCACGCGGA TGCGCCTGCA GATGCGCGTG CCTGCCTGCA GGTGGAGAGC
    GAGCCCGGAC TTCCTCCCCC GGCCTCCAGG GACCAGCCCT CCAAAGACAA GCCCAAGGTG
    AAGACGAAAG GGCGGCCCCG CGCCGCAGAC AGCCGGGAGC CCAAGCCGGC GGTGCCCACC
    CCCAGCGACC GGAAGAAGCA CCGCAGCGGC CCCCCGAAGG CGCCCCCGAA GGACGCGGCG
    GCAGAGGACC GGAGCCCCGA ACACTTTGCG CTCGTTCCCC TGACCCAGAG CCAGGGCCCT
    GTCCGCGGTG GCAGCGGCGG CGCCGGCGCC AGGACTAGTG GCTGCAGGCT GGCTGTGGTG
    GTCCAGGAGG ACCGCCGCAA AGACAAACCC CCGGTGCCTT CGAGAGACAC CAAGCTGCTC
    TCCCCGCTCA GGGACACTCC GCCGCCACAA AGCTTGGTGG TGAAGATCAC ACTCGACCTC
    CTGTCTCGGA TACCCCAGCC CCCAGGGAAG GGCAGCCGCC CCAAGAAACC AGAAGACAAA
    CAGCCACCAG CAGGGAAGAA GCCAGATCCT GAGAAGAGAA GCTCAGAAAA CGCCAGCAAG
    TTAGCCAAAA AGAGAAAGGG TGAAGCAGAG AAAGACCACG ATAGCAAGAA AGTCAGGCTG
    GAGAAGGAAG TCAAGTCACA GCCGTTGTCT TCCTCTTCCC ACAAGGAATC TTCCAAATCA
    AAGATGTCCA GGCCCTCCTC TGAGCCCTCA AAGAAGGAAA TGCTTCCCCC TTCGCTCGTG
    TCATCCTCAT CCTCCCAGAA GCCAGCCAAG CCTGCACAGA AGAGGCCACG GCAGGAAGAC
    CCCGGCTGCC AGGACCCCCA AAAAAGTGCC GGTAGTGCCA AGGGCAGCCA CAGAGACCCT
    TCTGCCTCCA AACACAGAAA AGCAGAGGGA AAGGTCTCTG GAAGCTCCAC AGAGCACAAA
    GGATCTTCTG GAGATACTGC AAACCGTTTT CCAGTGCCTT CTTTGCCAAA TGGTAACTCC
    AAACCAGGGA AGCCTCACGT GAAGTTTGAC AAACAACAAG CCGATTTTCA CCTGAAGGAA
    GCCAAAAGGT TGAAGGACAA AGCAGAATTA GTGACGGACA AGGTAGGGAA GGCCTTTAAA
    TACCTGGAGG CTGCCTTGTC CTTCATTGAG TGCGGCGTCG CCCTGGAGTC GGAGAGCCCC
    GCGTCCAAGT CGGCGTACTC CATCTACTCG GAGACCGTGG ACCTCATTAA GTTCATACTG
    TCATTAAAAT CCTTCTCAGA TGCCACAACG CCAACACAGG AGAAGATATT TGCTGTTCTA
    TGCATGCGTT GCCAGTCCAT TTTGAACATG GCAATGTTTC GTTGTAAAAA AGATATGGCG
    ATAAAGTATT CCCGCACGCT TAACGAACAC TTCTTCAAGA CTTCTTCCAA GGTTGCCCAG
    GCACCTTCTC CATGCATCGC AAGAAGCACA GGCACACCGT CCCCCCTCTC TCCGATCCCT
    TCTCCGGCCA GCTCCATAGG GTCCCAGTCA AGCGCTGGCA GCGTGGGGAG CGGGGCGCTG
    CCCGCCACCA TCAGCACCCC CGTCACCATC CAGAACATGA CATCCTCCTA CGTCAGCATC
    ACTTCCCACG TCCTTACCGC CTTTGACCTG TGGGAACAGG CTGAGGTCCT TACCAGGAAG
    AACAAAGAAT TCTTTGCTCA GCTCAGCACA AGTGTGTGCG CGCTGGCCCT CAACAGCAGT
    TTGATGGATC TGGTGCATTA TACAAGACAG GGTTTCCAGC GGCTAAAGCA AGTAACCAAG
    ACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804604.1
    CDS252..3920
    Translation

    Target ORF information:

    RefSeq Version XM_010806302.2
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806302.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCCC AGTCAAGCAG CTTGTACAAT GAAGACAGAA ACCTGCTTCG AATTAGAGAG 
    AAGGAAAGAC GCAACCAGGA AGCTCACCAA GATAAAGAGG CATTTTCTGA AAAGATCCCC
    CTTTTTGGAG AGCCATACAA GACAGAGAAA GGTGATGAGC TATCCAGTCG GATACAGAAC
    ATGCTGGGAA ACTATGAAGA GATGAAGGAG TTCCTTAGTA GCAAGTCCCA CCCCCAGCGC
    TTGGATGCTT CTGAAAATAG GCTGGGGAAG CCGAGATACC CTTTATTTCC TGAGAAGGGG
    AGCAGCCTTC CACCCCACTC CTTCCACACC AGTGTCCACC ACCAGCCCGT TAACACTCCT
    GCCTGTGGGC CACATCCTGC TGGTAACATC GGCCACCACC CAAAGATGGC GCAGCCAAGA
    ATGGAACCAA TGCCAAGTCT CCACGCCAAA AGCTATGGCC CACCGGACAG CCAGCACCTG
    ACCCAAGAGC GCCTCGGTCA GGAGGGGTAC GGCTCTAGTC ATCACAAAAA GGGTGACCGC
    AGGGCTGATG GAGACCACTG TGCTTTGACG ACAGACTCTG CTCCGGAGAG GGAACTTTCA
    CCTCTGTTCT CTTTGCCTTC CCCAGTCCCC CCTTTGTCAC CTGTACATTC CAACCAGCAG
    ACTGCTCCCA GGACGCAAGG AAGCGGCAAA GTTCCCAGCA GTAGCAGTAG CAATAAAGGC
    TACTGCCTGG CCAAGTCTCC CAAGGACCTA GCGGTAAAGG TGCACGATAA AGAGACCCCT
    CAAGACAGCA CAGGGGCAGT GGCCAGCCTT GGGGTAGCCC CTCCTCAGCC ACCTTCTCAG
    ACCTTCCCCC CACCCTCCCT CCCCTCAAAA AGCCTTGCAA TGCAGCAGAA GCCCACGGCT
    TATGTCCGGC CCATGGACGG TCAAGACCAG GCTCCTAGCG AGTCTCCTGA ACTGAAACCC
    CTGCCGGAGG AGTACCGGCA GCAGACCTTT GAGAAATCAG ACTTAAAAGT GCCTGCCAAA
    GCCAAGCTCA CGAAGCTGAA AATGCCTTCT CAGTCAGTCG AGCAGACCTA CTCCAATGAA
    GTCCATTGTG TTGAAGAGAT TCTGAAGGAA ATGACCCACT CATGGCCTCC TCCTTTGACA
    GCAATACACA CGCCTAGTAC AGCTGAGCCC TCCAAATTTC CTTTTCCCAC AAAGGATTCT
    CAGCACATCA GTTCTGCGGT CCCAAACCAA AAACAATATG ATACATCTTC AAAAACTCAC
    AGTAATTCTC AGCAGGGAAC ATCCATGCTC AAAGATGACC TTCAGCTCAG TGACAGTGAG
    GACAGTGACA GCGATCAAAC CTCAGAGAAG CCTCCTTTCG CACCTGCACC TCCAAGCGCT
    CCACCGGCGC TGCCAGCAGC CGCGGCCTCA GCACATTCCA GCAGCGCCGA ATCGGAGAGC
    ACCAGCGACT CCGACAGCTC CTCGGACTCG GAGAGCGAGA GCAGCTCCAG TGACAGCGAG
    GAGAACGAAC CCCGAGAAGC CCCGGCTCCC GAGCCCGAGC CTCCGACAAC CAACAAATGG
    CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCCGCGG TGCCCACCGA GGCCCCGGGT
    AGCACGGAGC CCCCGGCCCG ACCCCCGGAC ACTAAGGGCA AGGGCGGCGA CAGCGCCACC
    GCGGGTCACG AGCGCCCAGA GTCCAAAGAG CCTCCCCCCA AAGGCTCCAG CAAAGCCCCC
    CGGGTCCCAT CCGAAGGCCC CCACGCGGGC AAGAGGAGCT GTCAGAAGTC CCCTGCCCAG
    CAGGAGCCCC CCCAGCGGCA AACCGTGGGA AGCAAACAGC CGAAAAAGCC CATCAAGGCT
    TCCGCCCCCG GGCACGCGGA TGCGCCTGCA GATGCGCGTG CCTGCCTGCA GGTGGAGAGC
    GAGCCCGGAC TTCCTCCCCC GGCCTCCAGG GACCAGCCCT CCAAAGACAA GCCCAAGGTG
    AAGACGAAAG GGCGGCCCCG CGCCGCAGAC AGCCGGGAGC CCAAGCCGGC GGTGCCCACC
    CCCAGCGACC GGAAGAAGCA CCGCAGCGGC CCCCCGAAGG CGCCCCCGAA GGACGCGGCG
    GCAGAGGACC GGAGCCCCGA ACACTTTGCG CTCGTTCCCC TGACCCAGAG CCAGGGCCCT
    GTCCGCGGTG GCAGCGGCGG CGCCGGCGCC AGGACTAGTG GCTGCAGGCT GGCTGTGGTG
    GTCCAGGAGG ACCGCCGCAA AGACAAACCC CCGGTGCCTT CGAGAGACAC CAAGCTGCTC
    TCCCCGCTCA GGGACACTCC GCCGCCACAA AGCTTGGTGG TGAAGATCAC ACTCGACCTC
    CTGTCTCGGA TACCCCAGCC CCCAGGGAAG GGCAGCCGCC CCAAGAAACC AGAAGACAAA
    CAGCCACCAG CAGGGAAGAA GCCAGATCCT GAGAAGAGAA GCTCAGAAAA CGCCAGCAAG
    TTAGCCAAAA AGAGAAAGGG TGAAGCAGAG AAAGACCACG ATAGCAAGAA AGTCAGGCTG
    GAGAAGGAAG TCAAGTCACA GCCGTTGTCT TCCTCTTCCC ACAAGGAATC TTCCAAATCA
    AAGATGTCCA GGCCCTCCTC TGAGCCCTCA AAGAAGGAAA TGCTTCCCCC TTCGCTCGTG
    TCATCCTCAT CCTCCCAGAA GCCAGCCAAG CCTGCACAGA AGAGGCCACG GCAGGAAGAC
    CCCGGCTGCC AGGACCCCCA AAAAAGTGCC GGTAGTGCCA AGGGCAGCCA CAGAGACCCT
    TCTGCCTCCA AACACAGAAA AGCAGAGGGA AAGGTCTCTG GAAGCTCCAC AGAGCACAAA
    GGATCTTCTG GAGATACTGC AAACCGTTTT CCAGTGCCTT CTTTGCCAAA TGGTAACTCC
    AAACCAGGGA AGCCTCACGT GAAGTTTGAC AAACAACAAG CCGATTTTCA CCTGAAGGAA
    GCCAAAAGGT TGAAGGACAA AGCAGAATTA GTGACGGACA AGGTAGGGAA GGCCTTTAAA
    TACCTGGAGG CTGCCTTGTC CTTCATTGAG TGCGGCGTCG CCCTGGAGTC GGAGAGCCCC
    GCGTCCAAGT CGGCGTACTC CATCTACTCG GAGACCGTGG ACCTCATTAA GTTCATACTG
    TCATTAAAAT CCTTCTCAGA TGCCACAACG CCAACACAGG AGAAGATATT TGCTGTTCTA
    TGCATGCGTT GCCAGTCCAT TTTGAACATG GCAATGTTTC GTTGTAAAAA AGATATGGCG
    ATAAAGTATT CCCGCACGCT TAACGAACAC TTCTTCAAGA CTTCTTCCAA GGTTGCCCAG
    GCACCTTCTC CATGCATCGC AAGAAGCACA GGCACACCGT CCCCCCTCTC TCCGATCCCT
    TCTCCGGCCA GCTCCATAGG GTCCCAGTCA AGCGCTGGCA GCGTGGGGAG CGGGGCGCTG
    CCCGCCACCA TCAGCACCCC CGTCACCATC CAGAACATGA CATCCTCCTA CGTCAGCATC
    ACTTCCCACG TCCTTACCGC CTTTGACCTG TGGGAACAGG CTGAGGTCCT TACCAGGAAG
    AACAAAGAAT TCTTTGCTCA GCTCAGCACA AGTGTGTGCG CGCTGGCCCT CAACAGCAGT
    TTGATGGATC TGGTGCATTA TACAAGACAG GGTTTCCAGC GGCTAAAGCA AGTAACCAAG
    ACACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.