Mcf2l cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mcf2l
Entrez Gene ID	17207
Full Name	mcf.2 transforming sequence-like
Synonyms	C130040G20Rik,Dbs,Ost,mKIAA0362
General protein information	Preferred Names mcf.2 transforming sequence-like Names guanine nucleotide exchange factor DBS DBL's big sister MCF2-transforming sequence-like protein Ost gamma exchange factor for RhoA and Cdc42 guanine nucleotide exchange factor OSTIII
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 A1.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017312593.1	XP_017168082.1	guanine nucleotide exchange factor DBS isoform X1
XM_006508710.3	XP_006508773.1	guanine nucleotide exchange factor DBS isoform X4
XM_006508712.3	XP_006508775.1	guanine nucleotide exchange factor DBS isoform X6
XM_017312595.1	XP_017168084.1	guanine nucleotide exchange factor DBS isoform X14
NM_001159485.1	NP_001152957.1	guanine nucleotide exchange factor DBS isoform 3
NM_001371043.1	NP_001357972.1	guanine nucleotide exchange factor DBS isoform 4
NM_178076.3	NP_835177.2	guanine nucleotide exchange factor DBS isoform 1
XM_006508706.3	XP_006508769.1	guanine nucleotide exchange factor DBS isoform X1
XM_006508707.2	XP_006508770.1	guanine nucleotide exchange factor DBS isoform X1
XM_006508714.3	XP_006508777.1	guanine nucleotide exchange factor DBS isoform X8
XM_006508720.3	XP_006508783.1	guanine nucleotide exchange factor DBS isoform X15
NM_001371045.1	NP_001357974.1	guanine nucleotide exchange factor DBS isoform 6 precursor
NM_001159486.2	NP_001152958.1	guanine nucleotide exchange factor DBS isoform 2
NM_001371048.1	NP_001357977.1	guanine nucleotide exchange factor DBS isoform 9 precursor
NM_001371050.1	NP_001357979.1	guanine nucleotide exchange factor DBS isoform 11
NM_001159485.2	NP_001152957.1	guanine nucleotide exchange factor DBS isoform 3
NM_001371049.1	NP_001357978.1	guanine nucleotide exchange factor DBS isoform 10
XM_006508711.3	XP_006508774.1	guanine nucleotide exchange factor DBS isoform X5
XM_006508713.3	XP_006508776.1	guanine nucleotide exchange factor DBS isoform X7
XM_006508715.2	XP_006508778.1	guanine nucleotide exchange factor DBS isoform X9
NM_001159486.1	NP_001152958.1	guanine nucleotide exchange factor DBS isoform 2
XM_006508708.3	XP_006508771.1	guanine nucleotide exchange factor DBS isoform X2
XM_006508717.3	XP_006508780.1	guanine nucleotide exchange factor DBS isoform X11
XM_006508719.3	XP_006508782.1	guanine nucleotide exchange factor DBS isoform X13
XM_006508721.3	XP_006508784.1	guanine nucleotide exchange factor DBS isoform X16
XM_011242040.1	XP_011240342.1	guanine nucleotide exchange factor DBS isoform X1
XM_006508723.3	XP_006508786.1	guanine nucleotide exchange factor DBS isoform X18
NM_001371047.1	NP_001357976.1	guanine nucleotide exchange factor DBS isoform 8
NM_178076.4	NP_835177.2	guanine nucleotide exchange factor DBS isoform 1
XM_006508709.3	XP_006508772.1	guanine nucleotide exchange factor DBS isoform X3
XM_006508716.3	XP_006508779.1	guanine nucleotide exchange factor DBS isoform X10
XM_006508718.3	XP_006508781.1	guanine nucleotide exchange factor DBS isoform X12
XM_006508722.2	XP_006508785.1	guanine nucleotide exchange factor DBS isoform X17
NM_001371046.1	NP_001357975.1	guanine nucleotide exchange factor DBS isoform 7
NM_001371044.1	NP_001357973.1	guanine nucleotide exchange factor DBS isoform 5

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100497730	XP_002933052.2
Homo sapiens (human)	MCF2L	NP_001106203.2
Pan troglodytes (chimpanzee)	MCF2L	XP_003314263.1
	MCF2L	XP_001103476.2
Mus musculus (house mouse)	Mcf2l	NP_835177.2
Rattus norvegicus (Norway rat)	Mcf2l	NP_446403.2
Canis lupus familiaris (dog)	MCF2L	XP_005634173.1
Gallus gallus (chicken)	MCF2L	NP_001238992.1
Danio rerio (zebrafish)	mcf2la	XP_005171065.1
Bos taurus (cattle)	MCF2L	XP_005214085.1

Gene Ontology
Bibliography

Related articles in PubMed

Ptbp2 represses adult-specific splicing to regulate the generation of neuronal precursors in the embryonic brain.
Licatalosi DD, Yano M, Fak JJ, Mele A, Grabinski SE, Zhang C, Darnell RB
Genes & development26(14)1626-42(2012 Jul)

A mutation in the Srrm4 gene causes alternative splicing defects and deafness in the Bronx waltzer mouse.
Nakano Y, Jahan I, Bonde G, Sun X, Hildebrand MS, Engelhardt JF, Smith RJ, Cornell RA, Fritzsch B, B?nfi B
PLoS genetics8(10)e1002966(2012)

Release of autoinhibition of ASEF by APC leads to CDC42 activation and tumor suppression.
Mitin N, Betts L, Yohe ME, Der CJ, Sondek J, Rossman KL
Nature structural & molecular biology14(9)814-23(2007 Sep)

Role of the guanine nucleotide exchange factor Ost in negative regulation of receptor endocytosis by the small GTPase Rac1.
Ieguchi K, Ueda S, Kataoka T, Satoh T
The Journal of biological chemistry282(32)23296-305(2007 Aug)

The Dbs PH domain contributes independently to membrane targeting and regulation of guanine nucleotide-exchange activity.
Baumeister MA, Rossman KL, Sondek J, Lemmon MA
The Biochemical journal400(3)563-72(2006 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dbs transformation is associated with increased phosphorylation of myosin light chain and stress fiber formation, both of which occur in a ROCK-dependent manner
Title: Transformation by the Rho-specific guanine nucleotide exchange factor Dbs requires ROCK I-mediated phosphorylation of myosin light chain.

the Sec14 domain regulates Dbs transformation through at least two distinct mechanisms, neither of which appears to directly influence the in vivo exchange activity of the protein
Title: The Sec14 homology domain regulates the cellular distribution and transforming activity of the Rho-specific guanine nucleotide exchange factor Dbs.

Crystal structure of the DH/PH fragment of Dbs without bound GTPase
Title: Crystal structure of the DH/PH fragment of Dbs without bound GTPase.

Dbs has the potential to promote proliferation of immature thymocytes, but also sensitizes immature thymocytes to deletion.
Title: The RhoA- and CDC42-specific exchange factor Dbs promotes expansion of immature thymocytes and deletion of double-positive and single-positive thymocytes.

Dbs is activated by Rac1 at the pleckstrin homology domain
Title: Pleckstrin homology domain-mediated activation of the rho-specific guanine nucleotide exchange factor Dbs by Rac1.

The following Mcf2l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mcf2l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07073	NM_178076.4 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$692.30 $989.00
OMu82370	NM_001371044.1 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu84904	NM_001371045.1 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu84905	NM_001371043.1 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu84906	NM_001371048.1 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu84907	NM_001371050.1 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu84909	NM_001371047.1 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu84911	NM_001371046.1 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu84912	NM_001371049.1 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu82370	XM_017312595.1 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu07468	NM_001159485.2 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu07470	NM_001159486.2 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu40334	XM_011242040.1 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40334	XM_006508707.2 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40334	XM_017312593.1 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40334	XM_006508706.3 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40335	XM_006508708.3 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40336	XM_006508709.3 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40337	XM_006508710.3 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40338	XM_006508711.3 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40339	XM_006508712.3 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40340	XM_006508713.3 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40341	XM_006508714.3 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40342	XM_006508715.2 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40343	XM_006508716.3 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40344	XM_006508717.3 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40345	XM_006508718.3 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu40346	XM_006508719.3 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu40347	XM_006508720.3 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu40348	XM_006508721.3 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu40349	XM_006508722.2 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu40350	XM_006508723.3 Latest version!	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu07468	NM_001159485.1	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu07470	NM_001159486.1	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu07073	NM_178076.3	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$692.30 $989.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mcf2l ( NM_178076.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_835177.2 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07073
Clone ID Related Accession (Same CDS sequence)	NM_178076.3 , NM_178076.4
Accession Version	NM_178076.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ159636.1, AK082849.1, BC058622.1 and AI853669.1. On Apr 18, 2009 this sequence version replaced NM_178076.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). CCDS Note: The coding region has been updated to extend the N-terminus to one that is supported by available conservation data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058622.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGACGATGC GGTCCCTAGT TCTACCTCTG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC CTCAAAGGTC AGAAAGCCTC TCCGACCAGT 
CCTGACAAAA AAGCTAAGCG GCACGAAGTA AAGAGCGACC CGACTCCCTT TGGTTTGCGA 
GGCTGGAGCA AAACATCCCA CTCCCTGGAG GCCCCTGAGG AGGACGGAGG CTGGTCCAGT 
GCTGAGGAAC TCATCAACTC ATCTGATGCT GAGGAAGACG GTGGAGTGGG CCCTAAGAAG 
CTGGTGCCGG GTAAATACAC AGTGGTGATG GATGATGAGA AAGGGGGCCC TGACACCCTT 
GCCATGAGGA GTGGAGATAT GGTGGAGGTG GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT 
GTTCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT ATCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG GTAAGATCCA CTGTGCGCGC 
CAGCTGTGCC CTGAGCCTGC CGAGATCCTC AGCCCAGAGC CCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_835177.2
CDS	84..3611
Translation	MRFWLRNEEMALEEMVQRLNAVSKNTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGKIHCARQLCPEPAEILSPEPV

Target ORF information:

RefSeq Version	NM_178076.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178076.3

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGACGATGC GGTCCCTAGT TCTACCTCTG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC CTCAAAGGTC AGAAAGCCTC TCCGACCAGT 
CCTGACAAAA AAGCTAAGCG GCACGAAGTA AAGAGCGACC CGACTCCCTT TGGTTTGCGA 
GGCTGGAGCA AAACATCCCA CTCCCTGGAG GCCCCTGAGG AGGACGGAGG CTGGTCCAGT 
GCTGAGGAAC TCATCAACTC ATCTGATGCT GAGGAAGACG GTGGAGTGGG CCCTAAGAAG 
CTGGTGCCGG GTAAATACAC AGTGGTGATG GATGATGAGA AAGGGGGCCC TGACACCCTT 
GCCATGAGGA GTGGAGATAT GGTGGAGGTG GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT 
GTTCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT ATCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG GTAAGATCCA CTGTGCGCGC 
CAGCTGTGCC CTGAGCCTGC CGAGATCCTC AGCCCAGAGC CCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( NM_178076.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_835177.2 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 1, mRNA.

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CloneID	OMu07073
Clone ID Related Accession (Same CDS sequence)	NM_178076.3 , NM_178076.4
Accession Version	NM_178076.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-24
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127308.3. On Jun 25, 2019 this sequence version replaced NM_178076.3. Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: The coding region has been updated to extend the N-terminus to one that is supported by available conservation data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058622.1, SRR7345562.1120612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGACGATGC GGTCCCTAGT TCTACCTCTG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC CTCAAAGGTC AGAAAGCCTC TCCGACCAGT 
CCTGACAAAA AAGCTAAGCG GCACGAAGTA AAGAGCGACC CGACTCCCTT TGGTTTGCGA 
GGCTGGAGCA AAACATCCCA CTCCCTGGAG GCCCCTGAGG AGGACGGAGG CTGGTCCAGT 
GCTGAGGAAC TCATCAACTC ATCTGATGCT GAGGAAGACG GTGGAGTGGG CCCTAAGAAG 
CTGGTGCCGG GTAAATACAC AGTGGTGATG GATGATGAGA AAGGGGGCCC TGACACCCTT 
GCCATGAGGA GTGGAGATAT GGTGGAGGTG GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT 
GTTCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT ATCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG GTAAGATCCA CTGTGCGCGC 
CAGCTGTGCC CTGAGCCTGC CGAGATCCTC AGCCCAGAGC CCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_835177.2
CDS	66..3593
Translation	MRFWLRNEEMALEEMVQRLNAVSKNTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGKIHCARQLCPEPAEILSPEPV

Target ORF information:

RefSeq Version	NM_178076.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178076.4

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGACGATGC GGTCCCTAGT TCTACCTCTG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC CTCAAAGGTC AGAAAGCCTC TCCGACCAGT 
CCTGACAAAA AAGCTAAGCG GCACGAAGTA AAGAGCGACC CGACTCCCTT TGGTTTGCGA 
GGCTGGAGCA AAACATCCCA CTCCCTGGAG GCCCCTGAGG AGGACGGAGG CTGGTCCAGT 
GCTGAGGAAC TCATCAACTC ATCTGATGCT GAGGAAGACG GTGGAGTGGG CCCTAAGAAG 
CTGGTGCCGG GTAAATACAC AGTGGTGATG GATGATGAGA AAGGGGGCCC TGACACCCTT 
GCCATGAGGA GTGGAGATAT GGTGGAGGTG GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT 
GTTCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT ATCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG GTAAGATCCA CTGTGCGCGC 
CAGCTGTGCC CTGAGCCTGC CGAGATCCTC AGCCCAGAGC CCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( NM_001371044.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001357973.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu82370
Clone ID Related Accession (Same CDS sequence)	XM_017312595.1 , NM_001371044.1
Accession Version	NM_001371044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-24
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127308.3. On Jun 25, 2019 this sequence version replaced XM_017312595.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.179594.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAGGCC 
CCTGAGGAGG ACGGAGGCTG GTCCAGTGCT GAGGAACTCA TCAACTCATC TGATGCTGAG 
GAAGACGGTG GAGTGGGCCC TAAGAAGCTG GTGCCGGGTA AATACACAGT GGTGATGGAT 
GATGAGAAAG GGGGCCCTGA CACCCTTGCC ATGAGGAGTG GAGATATGGT GGAGGTGGTG 
GAAGAAGGCG CTGAGGGCCT CTGGTATGTT CGGGACCTGA CCAGCAGCAA GGAGGGATGG 
GTGCCAGCCA GCAGCCTATC CACCCTCCTG GGCAAATCCA GTTCAGCCCA GTGTCTGAGC 
AGCTCAGACT CCAGCCCAGG GTCAGCCGTG CTGAGCAGCT CATCCAGCTT CAGCGAGGGC 
TACCCTGCTC CCTTCTCCGA GCTCCAGGGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357973.1
CDS	66..3518
Translation	MRFWLRNEEMALEEMVQRLNAVSKNTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	NM_001371044.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371044.1

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAGGCC 
CCTGAGGAGG ACGGAGGCTG GTCCAGTGCT GAGGAACTCA TCAACTCATC TGATGCTGAG 
GAAGACGGTG GAGTGGGCCC TAAGAAGCTG GTGCCGGGTA AATACACAGT GGTGATGGAT 
GATGAGAAAG GGGGCCCTGA CACCCTTGCC ATGAGGAGTG GAGATATGGT GGAGGTGGTG 
GAAGAAGGCG CTGAGGGCCT CTGGTATGTT CGGGACCTGA CCAGCAGCAA GGAGGGATGG 
GTGCCAGCCA GCAGCCTATC CACCCTCCTG GGCAAATCCA GTTCAGCCCA GTGTCTGAGC 
AGCTCAGACT CCAGCCCAGG GTCAGCCGTG CTGAGCAGCT CATCCAGCTT CAGCGAGGGC 
TACCCTGCTC CCTTCTCCGA GCTCCAGGGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( NM_001371045.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001357974.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 6, mRNA.

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CloneID	OMu84904
Clone ID Related Accession (Same CDS sequence)	NM_001371045.1
Accession Version	NM_001371045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-24
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform 6 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127308.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR6116558.109946.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGACTTTGA GCCTCATCTC CGTGCTGTCC CAGGACGTCA CAGCCCTGTG GCTCCTGCTG 
AAGACAAGTG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC CGCTGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGACA GGACGGAAGC 
CCAGTCATCA CCTTCCCTGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATC 
TTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCTGTGA AGGCATCCGT CCTGCGGATA 
GCCGCATCTT TCCCAGCCAA CCTGCAGCTT GTCCTTGTTC TTCGGCCCAC GGGCTTTTTC 
CAGAGGACTC TCTCGGACAT TGCCTTCAAG TTCAACAGAG ATGAGTTTAA GATGAAAGTG 
CCGGTCATGA TGCTAAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCGCAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCACTCCA GGTGGCTGTG CCACCGCACA 
GCAATTGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCCC AGATGCTGCA GGCCTTTGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCGAAT GACGTCCAGT CAACCAGCCT GGTGCTCAGC 
GCACACACAG AGAAGAAGGC CAAAGTGAAG GAAGATCTGC AGTTAGCACT GAAGGAGGGG 
AACAGCATCC TGGAGAGCCT CAGAGAGCCA CTGGCTGAGA GCGCGGCCCA CAGTGTGAAC 
CAGGACCAGC TGGACAATCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAGACG 
GAGGCTGCCT TTGACGAATT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAAGG TTTCCGGGAG GTCAAAACCA CCTTGGATTC CATGTCCCAG 
AAGATAGCTG CTTTCACGGA TGTTGGCAAC AGCCTGGCCC ACGTGCAGCA CCTTCTGAAG 
GACTTGACCG CCTTTGAGGA GAAGTCCAGT GTGGCTGTGG ACAAGGCCCG CGCCCTGTCA 
CTGGAAGGTC AGCAGCTGAT TGAGAACAGG CACTACGCTG TGGACTCCAT CCACCCCAAG 
TGTGAAGAGC TCCAGCACCT CTGTGACCAC TTCGCCTCTG AGGTCACCAG GAGGCGGGGC 
CTGCTCAGCA AGTCCTTGGA GCTGCATAGC CTCCTGGAGA CGTCCATGAA GTGGAGCGAC 
GAGGGGATCT TCCTGCTGGC CTCGCAGCCT GTGGACAAAT GCCAGTCCCA GGATGGCGCA 
GAGGCGGCCC TCCAGGAGAT TGAGAAGTTT CTGGAGACCG GTGCAGAAAA TAAGATTCAG 
GAGCTCAATG AGATTTACAA GGAATACGAA TGCATCCTCA ACCAGGACCT TCTGGAACAC 
GTGCAAAAAG TCTTTCAGAA GCAAGAAAGC ACGGAGGAGA TGTTCCACCG CAGGCAAGCC 
AGCCTGAAGA AACTGGCAGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCCCGACCA 
GAGGCGCTTA CCAAGTCGCC CTCCCCCTCG CCAGGCTCCT GGCGAAGCTC TGAGAACTCC 
AGCTCTGAGG GCAACGCACT CCGCAGAGGG CCCTACAGGA GAGCCAAGAG CGAAATGAGT 
GAACCCCGGC AGGGCCGCAC CAGCTCCACA GGGGAAGAGG AAGAGAGCCT GGCCATCCTG 
CGCAGACACG TGATGAATGA GCTTCTGGAC ACCGAGCGGG CCTACGTGGA GGAGCTGCTC 
TGTGTCTTAG AGGGCTATGC CGCAGAGATG GATAACCCCT TGATGGCACA CCTCATCTCA 
ACAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAAAT CTATCATTTT 
CACAACAGAA TATTCCTGCG GGAGCTGGAG AGCTGCATAG ACTGCCCAGA GCTGGTGGGG 
AGGTGCTTTC TGGAAAGGAT GGAGGAGTTC CAGATCTATG AGAAGTACTG TCAGAACAAG 
CCACGCTCTG AGAGCCTGTG GAGGCAGTGC TCTGACTGCC CCTTCTTCCA GGAGTGTCAG 
AAGAAGCTGG ACCACAAGCT GAGCCTGGAC TCCTACCTGC TGAAACCTGT GCAGAGGATA 
ACCAAGTACC AGCTGCTGCT CAAGGAAATG CTGAAGTACA GCAAGCACTG TGAAGGGGCA 
GAGGACCTGC AGGAGGCGCT GAGCTCCATC CTGGGCATCC TCAAGGCTGT GAACGACTCC 
ATGCATCTCA TCGCCATCAC TGGCTATGAT GGAAACCTTG GCGACCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGCGT GTGGACAGAC CACAAGAAGG GCCACACCAA AGTGAAGGAG 
CTAGCCAGGT TCAAGCCCAT GCAGCGGCAC CTGTTTCTGC ACGAGAAGGC CGTCCTCTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGA TACGAGAAGG CTCCTTCCTA CAGCTACAAG 
CAGTCCTTGA ATATGACGGC TGTCGGCATC ACAGAGAACG TGAAGGGTGA CACCAAGAAG 
TTCGAGATCT GGTACAACGC AAGGGAGGAG GTTTATATCA TTCAGGCGCC AACCCCCGAG 
ATTAAAGCAG CGTGGGTGAA TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCTTGC 
CGGGAGGCCA GCCAGCACCG AGCCCTGGAG CAGTCCCACA GCCTTCCTTT GCCCACGCCA 
TCCAGCACCA GTCCCACAAA AGGGAACACA AGAAATGTCA AGAAGCTCGA AGACCGGAAG 
ACGGACCCGC TCAGCCTGGA AGGCTACGTG AGCTCATCGT TACCAAAGCC CCCTGAGAAG 
GGCAAAGGCT GGAGCAAAAC ATCCCACTCC CTGGAGGCCC CTGAGGAGGA CGGAGGCTGG 
TCCAGTGCTG AGGAACTCAT CAACTCATCT GATGCTGAGG AAGACGGTGG AGTGGGCCCT 
AAGAAGCTGG TGCCGGGTAA ATACACAGTG GTGATGGATG ATGAGAAAGG GGGCCCTGAC 
ACCCTTGCCA TGAGGAGTGG AGATATGGTG GAGGTGGTGG AAGAAGGCGC TGAGGGCCTC 
TGGTATGTTC GGGACCTGAC CAGCAGCAAG GAGGGATGGG TGCCAGCCAG CAGCCTATCC 
ACCCTCCTGG GCAAATCCAG TTCAGCCCAG TGTCTGAGCA GCTCAGGTAA GATCCACTGT 
GCGCGCCAGC TGTGCCCTGA GCCTGCCGAG ATCCTCAGCC CAGAGCCCGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357974.1
CDS	237..3590
Translation	MTLSLISVLSQDVTALWLLLKTSADEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGKIHCARQLCPEPAEILSPEPV

Target ORF information:

RefSeq Version	NM_001371045.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371045.1

ORF Insert Sequence:

ATGACTTTGA GCCTCATCTC CGTGCTGTCC CAGGACGTCA CAGCCCTGTG GCTCCTGCTG 
AAGACAAGTG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC CGCTGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGACA GGACGGAAGC 
CCAGTCATCA CCTTCCCTGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATC 
TTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCTGTGA AGGCATCCGT CCTGCGGATA 
GCCGCATCTT TCCCAGCCAA CCTGCAGCTT GTCCTTGTTC TTCGGCCCAC GGGCTTTTTC 
CAGAGGACTC TCTCGGACAT TGCCTTCAAG TTCAACAGAG ATGAGTTTAA GATGAAAGTG 
CCGGTCATGA TGCTAAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCGCAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCACTCCA GGTGGCTGTG CCACCGCACA 
GCAATTGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCCC AGATGCTGCA GGCCTTTGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCGAAT GACGTCCAGT CAACCAGCCT GGTGCTCAGC 
GCACACACAG AGAAGAAGGC CAAAGTGAAG GAAGATCTGC AGTTAGCACT GAAGGAGGGG 
AACAGCATCC TGGAGAGCCT CAGAGAGCCA CTGGCTGAGA GCGCGGCCCA CAGTGTGAAC 
CAGGACCAGC TGGACAATCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAGACG 
GAGGCTGCCT TTGACGAATT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAAGG TTTCCGGGAG GTCAAAACCA CCTTGGATTC CATGTCCCAG 
AAGATAGCTG CTTTCACGGA TGTTGGCAAC AGCCTGGCCC ACGTGCAGCA CCTTCTGAAG 
GACTTGACCG CCTTTGAGGA GAAGTCCAGT GTGGCTGTGG ACAAGGCCCG CGCCCTGTCA 
CTGGAAGGTC AGCAGCTGAT TGAGAACAGG CACTACGCTG TGGACTCCAT CCACCCCAAG 
TGTGAAGAGC TCCAGCACCT CTGTGACCAC TTCGCCTCTG AGGTCACCAG GAGGCGGGGC 
CTGCTCAGCA AGTCCTTGGA GCTGCATAGC CTCCTGGAGA CGTCCATGAA GTGGAGCGAC 
GAGGGGATCT TCCTGCTGGC CTCGCAGCCT GTGGACAAAT GCCAGTCCCA GGATGGCGCA 
GAGGCGGCCC TCCAGGAGAT TGAGAAGTTT CTGGAGACCG GTGCAGAAAA TAAGATTCAG 
GAGCTCAATG AGATTTACAA GGAATACGAA TGCATCCTCA ACCAGGACCT TCTGGAACAC 
GTGCAAAAAG TCTTTCAGAA GCAAGAAAGC ACGGAGGAGA TGTTCCACCG CAGGCAAGCC 
AGCCTGAAGA AACTGGCAGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCCCGACCA 
GAGGCGCTTA CCAAGTCGCC CTCCCCCTCG CCAGGCTCCT GGCGAAGCTC TGAGAACTCC 
AGCTCTGAGG GCAACGCACT CCGCAGAGGG CCCTACAGGA GAGCCAAGAG CGAAATGAGT 
GAACCCCGGC AGGGCCGCAC CAGCTCCACA GGGGAAGAGG AAGAGAGCCT GGCCATCCTG 
CGCAGACACG TGATGAATGA GCTTCTGGAC ACCGAGCGGG CCTACGTGGA GGAGCTGCTC 
TGTGTCTTAG AGGGCTATGC CGCAGAGATG GATAACCCCT TGATGGCACA CCTCATCTCA 
ACAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAAAT CTATCATTTT 
CACAACAGAA TATTCCTGCG GGAGCTGGAG AGCTGCATAG ACTGCCCAGA GCTGGTGGGG 
AGGTGCTTTC TGGAAAGGAT GGAGGAGTTC CAGATCTATG AGAAGTACTG TCAGAACAAG 
CCACGCTCTG AGAGCCTGTG GAGGCAGTGC TCTGACTGCC CCTTCTTCCA GGAGTGTCAG 
AAGAAGCTGG ACCACAAGCT GAGCCTGGAC TCCTACCTGC TGAAACCTGT GCAGAGGATA 
ACCAAGTACC AGCTGCTGCT CAAGGAAATG CTGAAGTACA GCAAGCACTG TGAAGGGGCA 
GAGGACCTGC AGGAGGCGCT GAGCTCCATC CTGGGCATCC TCAAGGCTGT GAACGACTCC 
ATGCATCTCA TCGCCATCAC TGGCTATGAT GGAAACCTTG GCGACCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGCGT GTGGACAGAC CACAAGAAGG GCCACACCAA AGTGAAGGAG 
CTAGCCAGGT TCAAGCCCAT GCAGCGGCAC CTGTTTCTGC ACGAGAAGGC CGTCCTCTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGA TACGAGAAGG CTCCTTCCTA CAGCTACAAG 
CAGTCCTTGA ATATGACGGC TGTCGGCATC ACAGAGAACG TGAAGGGTGA CACCAAGAAG 
TTCGAGATCT GGTACAACGC AAGGGAGGAG GTTTATATCA TTCAGGCGCC AACCCCCGAG 
ATTAAAGCAG CGTGGGTGAA TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCTTGC 
CGGGAGGCCA GCCAGCACCG AGCCCTGGAG CAGTCCCACA GCCTTCCTTT GCCCACGCCA 
TCCAGCACCA GTCCCACAAA AGGGAACACA AGAAATGTCA AGAAGCTCGA AGACCGGAAG 
ACGGACCCGC TCAGCCTGGA AGGCTACGTG AGCTCATCGT TACCAAAGCC CCCTGAGAAG 
GGCAAAGGCT GGAGCAAAAC ATCCCACTCC CTGGAGGCCC CTGAGGAGGA CGGAGGCTGG 
TCCAGTGCTG AGGAACTCAT CAACTCATCT GATGCTGAGG AAGACGGTGG AGTGGGCCCT 
AAGAAGCTGG TGCCGGGTAA ATACACAGTG GTGATGGATG ATGAGAAAGG GGGCCCTGAC 
ACCCTTGCCA TGAGGAGTGG AGATATGGTG GAGGTGGTGG AAGAAGGCGC TGAGGGCCTC 
TGGTATGTTC GGGACCTGAC CAGCAGCAAG GAGGGATGGG TGCCAGCCAG CAGCCTATCC 
ACCCTCCTGG GCAAATCCAG TTCAGCCCAG TGTCTGAGCA GCTCAGGTAA GATCCACTGT 
GCGCGCCAGC TGTGCCCTGA GCCTGCCGAG ATCCTCAGCC CAGAGCCCGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( NM_001371043.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001357972.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu84905
Clone ID Related Accession (Same CDS sequence)	NM_001371043.1
Accession Version	NM_001371043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC134581.5 and AC127308.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGGCTGGAG CAAAACATCC 
CACTCCCTGG AGGCCCCTGA GGAGGACGGA GGCTGGTCCA GTGCTGAGGA ACTCATCAAC 
TCATCTGATG CTGAGGAAGA CGGTGGAGTG GGCCCTAAGA AGCTGGTGCC GGGTAAATAC 
ACAGTGGTGA TGGATGATGA GAAAGGGGGC CCTGACACCC TTGCCATGAG GAGTGGAGAT 
ATGGTGGAGG TGGTGGAAGA AGGCGCTGAG GGCCTCTGGT ATGTTCGGGA CCTGACCAGC 
AGCAAGGAGG GATGGGTGCC AGCCAGCAGC CTATCCACCC TCCTGGGCAA ATCCAGTTCA 
GCCCAGTGTC TGAGCAGCTC AGGTAAGATC CACTGTGCGC GCCAGCTGTG CCCTGAGCCT 
GCCGAGATCC TCAGCCCAGA GCCCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357972.1
CDS	312..3761
Translation	MSDCWCFIFCKEHVRSNPLSPQHDGASREEADHQVDVSDGIRLVPDKAEATAATASDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGKIHCARQLCPEPAEILSPEPV

Target ORF information:

RefSeq Version	NM_001371043.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371043.1

ORF Insert Sequence:

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGGCTGGAG CAAAACATCC 
CACTCCCTGG AGGCCCCTGA GGAGGACGGA GGCTGGTCCA GTGCTGAGGA ACTCATCAAC 
TCATCTGATG CTGAGGAAGA CGGTGGAGTG GGCCCTAAGA AGCTGGTGCC GGGTAAATAC 
ACAGTGGTGA TGGATGATGA GAAAGGGGGC CCTGACACCC TTGCCATGAG GAGTGGAGAT 
ATGGTGGAGG TGGTGGAAGA AGGCGCTGAG GGCCTCTGGT ATGTTCGGGA CCTGACCAGC 
AGCAAGGAGG GATGGGTGCC AGCCAGCAGC CTATCCACCC TCCTGGGCAA ATCCAGTTCA 
GCCCAGTGTC TGAGCAGCTC AGGTAAGATC CACTGTGCGC GCCAGCTGTG CCCTGAGCCT 
GCCGAGATCC TCAGCCCAGA GCCCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( NM_001371048.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001357977.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OMu84906
Clone ID Related Accession (Same CDS sequence)	NM_001371048.1
Accession Version	NM_001371048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-24
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform 9 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127308.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7652917.1005079.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGACTTTGA GCCTCATCTC CGTGCTGTCC CAGGACGTCA CAGCCCTGTG GCTCCTGCTG 
AAGACAAGTG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC CGCTGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGACA GGACGGAAGC 
CCAGTCATCA CCTTCCCTGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATC 
TTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCTGTGA AGGCATCCGT CCTGCGGATA 
GCCGCATCTT TCCCAGCCAA CCTGCAGCTT GTCCTTGTTC TTCGGCCCAC GGGCTTTTTC 
CAGAGGACTC TCTCGGACAT TGCCTTCAAG TTCAACAGAG ATGAGTTTAA GATGAAAGTG 
CCGGTCATGA TGCTAAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCGCAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCACTCCA GGTGGCTGTG CCACCGCACA 
GCAATTGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCCC AGATGCTGCA GGCCTTTGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCGAAT GACGTCCAGT CAACCAGCCT GGTGCTCAGC 
GCACACACAG AGAAGAAGGC CAAAGTGAAG GAAGATCTGC AGTTAGCACT GAAGGAGGGG 
AACAGCATCC TGGAGAGCCT CAGAGAGCCA CTGGCTGAGA GCGCGGCCCA CAGTGTGAAC 
CAGGACCAGC TGGACAATCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAGACG 
GAGGCTGCCT TTGACGAATT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAAGG TTTCCGGGAG GTCAAAACCA CCTTGGATTC CATGTCCCAG 
AAGATAGCTG CTTTCACGGA TGTTGGCAAC AGCCTGGCCC ACGTGCAGCA CCTTCTGAAG 
GACTTGACCG CCTTTGAGGA GAAGTCCAGT GTGGCTGTGG ACAAGGCCCG CGCCCTGTCA 
CTGGAAGGTC AGCAGCTGAT TGAGAACAGG CACTACGCTG TGGACTCCAT CCACCCCAAG 
TGTGAAGAGC TCCAGCACCT CTGTGACCAC TTCGCCTCTG AGGTCACCAG GAGGCGGGGC 
CTGCTCAGCA AGTCCTTGGA GCTGCATAGC CTCCTGGAGA CGTCCATGAA GTGGAGCGAC 
GAGGGGATCT TCCTGCTGGC CTCGCAGCCT GTGGACAAAT GCCAGTCCCA GGATGGCGCA 
GAGGCGGCCC TCCAGGAGAT TGAGAAGTTT CTGGAGACCG GTGCAGAAAA TAAGATTCAG 
GAGCTCAATG AGATTTACAA GGAATACGAA TGCATCCTCA ACCAGGACCT TCTGGAACAC 
GTGCAAAAAG TCTTTCAGAA GCAAGAAAGC ACGGAGGAGA TGTTCCACCG CAGGCAAGCC 
AGCCTGAAGA AACTGGCAGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCCCGACCA 
GAGGCGCTTA CCAAGTCGCC CTCCCCCTCG CCAGGCTCCT GGCGAAGCTC TGAGAACTCC 
AGCTCTGAGG GCAACGCACT CCGCAGAGGG CCCTACAGGA GAGCCAAGAG CGAAATGAGT 
GAACCCCGGC AGGGCCGCAC CAGCTCCACA GGGGAAGAGG AAGAGAGCCT GGCCATCCTG 
CGCAGACACG TGATGAATGA GCTTCTGGAC ACCGAGCGGG CCTACGTGGA GGAGCTGCTC 
TGTGTCTTAG AGGGCTATGC CGCAGAGATG GATAACCCCT TGATGGCACA CCTCATCTCA 
ACAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAAAT CTATCATTTT 
CACAACAGAA TATTCCTGCG GGAGCTGGAG AGCTGCATAG ACTGCCCAGA GCTGGTGGGG 
AGGTGCTTTC TGGAAAGGAT GGAGGAGTTC CAGATCTATG AGAAGTACTG TCAGAACAAG 
CCACGCTCTG AGAGCCTGTG GAGGCAGTGC TCTGACTGCC CCTTCTTCCA GGAGTGTCAG 
AAGAAGCTGG ACCACAAGCT GAGCCTGGAC TCCTACCTGC TGAAACCTGT GCAGAGGATA 
ACCAAGTACC AGCTGCTGCT CAAGGAAATG CTGAAGTACA GCAAGCACTG TGAAGGGGCA 
GAGGACCTGC AGGAGGCGCT GAGCTCCATC CTGGGCATCC TCAAGGCTGT GAACGACTCC 
ATGCATCTCA TCGCCATCAC TGGCTATGAT GGAAACCTTG GCGACCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGCGT GTGGACAGAC CACAAGAAGG GCCACACCAA AGTGAAGGAG 
CTAGCCAGGT TCAAGCCCAT GCAGCGGCAC CTGTTTCTGC ACGAGAAGGC CGTCCTCTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGA TACGAGAAGG CTCCTTCCTA CAGCTACAAG 
CAGTCCTTGA ATATGACGGC TGTCGGCATC ACAGAGAACG TGAAGGGTGA CACCAAGAAG 
TTCGAGATCT GGTACAACGC AAGGGAGGAG GTTTATATCA TTCAGGCGCC AACCCCCGAG 
ATTAAAGCAG CGTGGGTGAA TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCTTGC 
CGGGAGGCCA GCCAGCACCG AGCCCTGGAG CAGTCCCACA GCCTTCCTTT GCCCACGCCA 
TCCAGCACCA GTCCCACAAA AGGGAACACA AGAAATGTCA AGAAGCTCGA AGACCGGAAG 
ACGGACCCGC TCAGCCTGGA AGGCTACGTG AGCTCATCGT TACCAAAGCC CCCTGAGAAG 
GGCAAAGACG ATGCGGTCCC TAGTTCTACC TCTGAAAGCT CTGCGCTTTC CAGAAAGCGC 
TTCACGCTGC AGGGCTTGGC TAACCTCAAA GGTCAGAAAG CCTCTCCGAC CAGTCCTGAC 
AAAAAAGCTA AGCGGCACGA AGTAAAGAGC GACCCGACTC CCTTTGGTTT GCGAGGCTGG 
AGCAAAACAT CCCACTCCCT GGAGGCCCCT GAGGAGGACG GAGGCTGGTC CAGTGCTGAG 
GAACTCATCA ACTCATCTGA TGCTGAGGAA GACGGTGGAG TGGGCCCTAA GAAGCTGGTG 
CCGGGTAAAT ACACAGTGGT GATGGATGAT GAGAAAGGGG GCCCTGACAC CCTTGCCATG 
AGGAGTGGAG ATATGGTGGA GGTGGTGGAA GAAGGCGCTG AGGGCCTCTG GTATGTTCGG 
GACCTGACCA GCAGCAAGGA GGGATGGGTG CCAGCCAGCA GCCTATCCAC CCTCCTGGGC 
AAATCCAGTT CAGCCCAGTG TCTGAGCAGC TCAGGTAAGA TCCACTGTGC GCGCCAGCTG 
TGCCCTGAGC CTGCCGAGAT CCTCAGCCCA GAGCCCGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357977.1
CDS	237..3758
Translation	MTLSLISVLSQDVTALWLLLKTSADEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGKIHCARQLCPEPAEILSPEPV

Target ORF information:

RefSeq Version	NM_001371048.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371048.1

ORF Insert Sequence:

ATGACTTTGA GCCTCATCTC CGTGCTGTCC CAGGACGTCA CAGCCCTGTG GCTCCTGCTG 
AAGACAAGTG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC CGCTGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGACA GGACGGAAGC 
CCAGTCATCA CCTTCCCTGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATC 
TTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCTGTGA AGGCATCCGT CCTGCGGATA 
GCCGCATCTT TCCCAGCCAA CCTGCAGCTT GTCCTTGTTC TTCGGCCCAC GGGCTTTTTC 
CAGAGGACTC TCTCGGACAT TGCCTTCAAG TTCAACAGAG ATGAGTTTAA GATGAAAGTG 
CCGGTCATGA TGCTAAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCGCAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCACTCCA GGTGGCTGTG CCACCGCACA 
GCAATTGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCCC AGATGCTGCA GGCCTTTGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCGAAT GACGTCCAGT CAACCAGCCT GGTGCTCAGC 
GCACACACAG AGAAGAAGGC CAAAGTGAAG GAAGATCTGC AGTTAGCACT GAAGGAGGGG 
AACAGCATCC TGGAGAGCCT CAGAGAGCCA CTGGCTGAGA GCGCGGCCCA CAGTGTGAAC 
CAGGACCAGC TGGACAATCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAGACG 
GAGGCTGCCT TTGACGAATT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAAGG TTTCCGGGAG GTCAAAACCA CCTTGGATTC CATGTCCCAG 
AAGATAGCTG CTTTCACGGA TGTTGGCAAC AGCCTGGCCC ACGTGCAGCA CCTTCTGAAG 
GACTTGACCG CCTTTGAGGA GAAGTCCAGT GTGGCTGTGG ACAAGGCCCG CGCCCTGTCA 
CTGGAAGGTC AGCAGCTGAT TGAGAACAGG CACTACGCTG TGGACTCCAT CCACCCCAAG 
TGTGAAGAGC TCCAGCACCT CTGTGACCAC TTCGCCTCTG AGGTCACCAG GAGGCGGGGC 
CTGCTCAGCA AGTCCTTGGA GCTGCATAGC CTCCTGGAGA CGTCCATGAA GTGGAGCGAC 
GAGGGGATCT TCCTGCTGGC CTCGCAGCCT GTGGACAAAT GCCAGTCCCA GGATGGCGCA 
GAGGCGGCCC TCCAGGAGAT TGAGAAGTTT CTGGAGACCG GTGCAGAAAA TAAGATTCAG 
GAGCTCAATG AGATTTACAA GGAATACGAA TGCATCCTCA ACCAGGACCT TCTGGAACAC 
GTGCAAAAAG TCTTTCAGAA GCAAGAAAGC ACGGAGGAGA TGTTCCACCG CAGGCAAGCC 
AGCCTGAAGA AACTGGCAGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCCCGACCA 
GAGGCGCTTA CCAAGTCGCC CTCCCCCTCG CCAGGCTCCT GGCGAAGCTC TGAGAACTCC 
AGCTCTGAGG GCAACGCACT CCGCAGAGGG CCCTACAGGA GAGCCAAGAG CGAAATGAGT 
GAACCCCGGC AGGGCCGCAC CAGCTCCACA GGGGAAGAGG AAGAGAGCCT GGCCATCCTG 
CGCAGACACG TGATGAATGA GCTTCTGGAC ACCGAGCGGG CCTACGTGGA GGAGCTGCTC 
TGTGTCTTAG AGGGCTATGC CGCAGAGATG GATAACCCCT TGATGGCACA CCTCATCTCA 
ACAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAAAT CTATCATTTT 
CACAACAGAA TATTCCTGCG GGAGCTGGAG AGCTGCATAG ACTGCCCAGA GCTGGTGGGG 
AGGTGCTTTC TGGAAAGGAT GGAGGAGTTC CAGATCTATG AGAAGTACTG TCAGAACAAG 
CCACGCTCTG AGAGCCTGTG GAGGCAGTGC TCTGACTGCC CCTTCTTCCA GGAGTGTCAG 
AAGAAGCTGG ACCACAAGCT GAGCCTGGAC TCCTACCTGC TGAAACCTGT GCAGAGGATA 
ACCAAGTACC AGCTGCTGCT CAAGGAAATG CTGAAGTACA GCAAGCACTG TGAAGGGGCA 
GAGGACCTGC AGGAGGCGCT GAGCTCCATC CTGGGCATCC TCAAGGCTGT GAACGACTCC 
ATGCATCTCA TCGCCATCAC TGGCTATGAT GGAAACCTTG GCGACCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGCGT GTGGACAGAC CACAAGAAGG GCCACACCAA AGTGAAGGAG 
CTAGCCAGGT TCAAGCCCAT GCAGCGGCAC CTGTTTCTGC ACGAGAAGGC CGTCCTCTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGA TACGAGAAGG CTCCTTCCTA CAGCTACAAG 
CAGTCCTTGA ATATGACGGC TGTCGGCATC ACAGAGAACG TGAAGGGTGA CACCAAGAAG 
TTCGAGATCT GGTACAACGC AAGGGAGGAG GTTTATATCA TTCAGGCGCC AACCCCCGAG 
ATTAAAGCAG CGTGGGTGAA TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCTTGC 
CGGGAGGCCA GCCAGCACCG AGCCCTGGAG CAGTCCCACA GCCTTCCTTT GCCCACGCCA 
TCCAGCACCA GTCCCACAAA AGGGAACACA AGAAATGTCA AGAAGCTCGA AGACCGGAAG 
ACGGACCCGC TCAGCCTGGA AGGCTACGTG AGCTCATCGT TACCAAAGCC CCCTGAGAAG 
GGCAAAGACG ATGCGGTCCC TAGTTCTACC TCTGAAAGCT CTGCGCTTTC CAGAAAGCGC 
TTCACGCTGC AGGGCTTGGC TAACCTCAAA GGTCAGAAAG CCTCTCCGAC CAGTCCTGAC 
AAAAAAGCTA AGCGGCACGA AGTAAAGAGC GACCCGACTC CCTTTGGTTT GCGAGGCTGG 
AGCAAAACAT CCCACTCCCT GGAGGCCCCT GAGGAGGACG GAGGCTGGTC CAGTGCTGAG 
GAACTCATCA ACTCATCTGA TGCTGAGGAA GACGGTGGAG TGGGCCCTAA GAAGCTGGTG 
CCGGGTAAAT ACACAGTGGT GATGGATGAT GAGAAAGGGG GCCCTGACAC CCTTGCCATG 
AGGAGTGGAG ATATGGTGGA GGTGGTGGAA GAAGGCGCTG AGGGCCTCTG GTATGTTCGG 
GACCTGACCA GCAGCAAGGA GGGATGGGTG CCAGCCAGCA GCCTATCCAC CCTCCTGGGC 
AAATCCAGTT CAGCCCAGTG TCTGAGCAGC TCAGGTAAGA TCCACTGTGC GCGCCAGCTG 
TGCCCTGAGC CTGCCGAGAT CCTCAGCCCA GAGCCCGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( NM_001371050.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001357979.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OMu84907
Clone ID Related Accession (Same CDS sequence)	NM_001371050.1
Accession Version	NM_001371050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-24
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform 11
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127308.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7652917.1081108.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCAGAAA AGGGTGCGTC CCGGGGGGCC GGGCGGCGGT TCTGGTCACT ATCAAGGAAG 
CGTCGGGGAG CACCAGGGCG CCACAAGCCA GATGAAATCA TGCACCAGGA CATCGTCCCG 
CTCTGTGCCG CTGACATCCA GGAGCAGCTG AAGAAGCGCT TTGCTTACCT GTCTGGTGGG 
CGGGGACAGG ACGGAAGCCC AGTCATCACC TTCCCTGACT ACCCAGCCTT CAGCGAGATC 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TATCTCACCA GCATCCCCAG TTTGCAAGAT 
GCTGGCATTG GATTCATCTT GGTCATAGAC CGAAGGCAGG ACAAATGGAC CTCTGTGAAG 
GCATCCGTCC TGCGGATAGC CGCATCTTTC CCAGCCAACC TGCAGCTTGT CCTTGTTCTT 
CGGCCCACGG GCTTTTTCCA GAGGACTCTC TCGGACATTG CCTTCAAGTT CAACAGAGAT 
GAGTTTAAGA TGAAAGTGCC GGTCATGATG CTAAGCTCAG TGCCAGAACT ACACGGCTAC 
ATTGATAAGT CGCAGCTGAC CGAGGACCTC GGGGGGACCC TGGACTACTG CCACTCCAGG 
TGGCTGTGCC ACCGCACAGC AATTGAAAGC TTCGCCCTGA TGGTGAAGCA GACAGCCCAG 
ATGCTGCAGG CCTTTGGGAC CGAGCTGGCT GAAACAGAGC TGCCGAATGA CGTCCAGTCA 
ACCAGCCTGG TGCTCAGCGC ACACACAGAG AAGAAGGCCA AAGTGAAGGA AGATCTGCAG 
TTAGCACTGA AGGAGGGGAA CAGCATCCTG GAGAGCCTCA GAGAGCCACT GGCTGAGAGC 
GCGGCCCACA GTGTGAACCA GGACCAGCTG GACAATCAGG CCACCGTGCA GAGGCTCCTG 
GCCCAGCTGA ATGAGACGGA GGCTGCCTTT GACGAATTCT GGGCAAAGCA TCAGCAGAAG 
CTGGAACAGT GCCTGCAGCT GCGGCATTTT GAGCAAGGTT TCCGGGAGGT CAAAACCACC 
TTGGATTCCA TGTCCCAGAA GATAGCTGCT TTCACGGATG TTGGCAACAG CCTGGCCCAC 
GTGCAGCACC TTCTGAAGGA CTTGACCGCC TTTGAGGAGA AGTCCAGTGT GGCTGTGGAC 
AAGGCCCGCG CCCTGTCACT GGAAGGTCAG CAGCTGATTG AGAACAGGCA CTACGCTGTG 
GACTCCATCC ACCCCAAGTG TGAAGAGCTC CAGCACCTCT GTGACCACTT CGCCTCTGAG 
GTCACCAGGA GGCGGGGCCT GCTCAGCAAG TCCTTGGAGC TGCATAGCCT CCTGGAGACG 
TCCATGAAGT GGAGCGACGA GGGGATCTTC CTGCTGGCCT CGCAGCCTGT GGACAAATGC 
CAGTCCCAGG ATGGCGCAGA GGCGGCCCTC CAGGAGATTG AGAAGTTTCT GGAGACCGGT 
GCAGAAAATA AGATTCAGGA GCTCAATGAG ATTTACAAGG AATACGAATG CATCCTCAAC 
CAGGACCTTC TGGAACACGT GCAAAAAGTC TTTCAGAAGC AAGAAAGCAC GGAGGAGATG 
TTCCACCGCA GGCAAGCCAG CCTGAAGAAA CTGGCAGCCA AGCAGACACG GCCTGTGCAG 
CCCGTGGCCC CCCGACCAGA GGCGCTTACC AAGTCGCCCT CCCCCTCGCC AGGCTCCTGG 
CGAAGCTCTG AGAACTCCAG CTCTGAGGGC AACGCACTCC GCAGAGGGCC CTACAGGAGA 
GCCAAGAGCG AAATGAGTGA ACCCCGGCAG GGCCGCACCA GCTCCACAGG GGAAGAGGAA 
GAGAGCCTGG CCATCCTGCG CAGACACGTG ATGAATGAGC TTCTGGACAC CGAGCGGGCC 
TACGTGGAGG AGCTGCTCTG TGTCTTAGAG GGCTATGCCG CAGAGATGGA TAACCCCTTG 
ATGGCACACC TCATCTCAAC AGGCCTGCAA AACAAGAAGA ACATTCTGTT TGGAAACATG 
GAGGAAATCT ATCATTTTCA CAACAGAATA TTCCTGCGGG AGCTGGAGAG CTGCATAGAC 
TGCCCAGAGC TGGTGGGGAG GTGCTTTCTG GAAAGGATGG AGGAGTTCCA GATCTATGAG 
AAGTACTGTC AGAACAAGCC ACGCTCTGAG AGCCTGTGGA GGCAGTGCTC TGACTGCCCC 
TTCTTCCAGG AGTGTCAGAA GAAGCTGGAC CACAAGCTGA GCCTGGACTC CTACCTGCTG 
AAACCTGTGC AGAGGATAAC CAAGTACCAG CTGCTGCTCA AGGAAATGCT GAAGTACAGC 
AAGCACTGTG AAGGGGCAGA GGACCTGCAG GAGGCGCTGA GCTCCATCCT GGGCATCCTC 
AAGGCTGTGA ACGACTCCAT GCATCTCATC GCCATCACTG GCTATGATGG AAACCTTGGC 
GACCTGGGGA AGCTGCTGAT GCAAGGTTCC TTCAGCGTGT GGACAGACCA CAAGAAGGGC 
CACACCAAAG TGAAGGAGCT AGCCAGGTTC AAGCCCATGC AGCGGCACCT GTTTCTGCAC 
GAGAAGGCCG TCCTCTTCTG CAAGAAGAGG GAGGAGAATG GGGAGGGATA CGAGAAGGCT 
CCTTCCTACA GCTACAAGCA GTCCTTGAAT ATGACGGCTG TCGGCATCAC AGAGAACGTG 
AAGGGTGACA CCAAGAAGTT CGAGATCTGG TACAACGCAA GGGAGGAGGT TTATATCATT 
CAGGCGCCAA CCCCCGAGAT TAAAGCAGCG TGGGTGAATG AGATTCGGAA GGTGCTGACC 
AGCCAGCTGC AGGCTTGCCG GGAGGCCAGC CAGCACCGAG CCCTGGAGCA GTCCCACAGC 
CTTCCTTTGC CCACGCCATC CAGCACCAGT CCCACAAAAG GGAACACAAG AAATGTCAAG 
AAGCTCGAAG ACCGGAAGAC GGACCCGCTC AGCCTGGAAG GCTACGTGAG CTCATCGTTA 
CCAAAGCCCC CTGAGAAGGG CAAAGGCTGG AGCAAAACAT CCCACTCCCT GGAGGCCCCT 
GAGGAGGACG GAGGCTGGTC CAGTGCTGAG GAACTCATCA ACTCATCTGA TGCTGAGGAA 
GACGGTGGAG TGGGCCCTAA GAAGCTGGTG CCGGGTAAAT ACACAGTGGT GATGGATGAT 
GAGAAAGGGG GCCCTGACAC CCTTGCCATG AGGAGTGGAG ATATGGTGGA GGTGGTGGAA 
GAAGGCGCTG AGGGCCTCTG GTATGTTCGG GACCTGACCA GCAGCAAGGA GGGATGGGTG 
CCAGCCAGCA GCCTATCCAC CCTCCTGGGC AAATCCAGTT CAGCCCAGTG TCTGAGCAGC 
TCAGGTAAGA TCCACTGTGC GCGCCAGCTG TGCCCTGAGC CTGCCGAGAT CCTCAGCCCA 
GAGCCCGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357979.1
CDS	368..3739
Translation	MAEKGASRGAGRRFWSLSRKRRGAPGRHKPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGKIHCARQLCPEPAEILSPEPV

Target ORF information:

RefSeq Version	NM_001371050.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371050.1

ORF Insert Sequence:

ATGGCAGAAA AGGGTGCGTC CCGGGGGGCC GGGCGGCGGT TCTGGTCACT ATCAAGGAAG 
CGTCGGGGAG CACCAGGGCG CCACAAGCCA GATGAAATCA TGCACCAGGA CATCGTCCCG 
CTCTGTGCCG CTGACATCCA GGAGCAGCTG AAGAAGCGCT TTGCTTACCT GTCTGGTGGG 
CGGGGACAGG ACGGAAGCCC AGTCATCACC TTCCCTGACT ACCCAGCCTT CAGCGAGATC 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TATCTCACCA GCATCCCCAG TTTGCAAGAT 
GCTGGCATTG GATTCATCTT GGTCATAGAC CGAAGGCAGG ACAAATGGAC CTCTGTGAAG 
GCATCCGTCC TGCGGATAGC CGCATCTTTC CCAGCCAACC TGCAGCTTGT CCTTGTTCTT 
CGGCCCACGG GCTTTTTCCA GAGGACTCTC TCGGACATTG CCTTCAAGTT CAACAGAGAT 
GAGTTTAAGA TGAAAGTGCC GGTCATGATG CTAAGCTCAG TGCCAGAACT ACACGGCTAC 
ATTGATAAGT CGCAGCTGAC CGAGGACCTC GGGGGGACCC TGGACTACTG CCACTCCAGG 
TGGCTGTGCC ACCGCACAGC AATTGAAAGC TTCGCCCTGA TGGTGAAGCA GACAGCCCAG 
ATGCTGCAGG CCTTTGGGAC CGAGCTGGCT GAAACAGAGC TGCCGAATGA CGTCCAGTCA 
ACCAGCCTGG TGCTCAGCGC ACACACAGAG AAGAAGGCCA AAGTGAAGGA AGATCTGCAG 
TTAGCACTGA AGGAGGGGAA CAGCATCCTG GAGAGCCTCA GAGAGCCACT GGCTGAGAGC 
GCGGCCCACA GTGTGAACCA GGACCAGCTG GACAATCAGG CCACCGTGCA GAGGCTCCTG 
GCCCAGCTGA ATGAGACGGA GGCTGCCTTT GACGAATTCT GGGCAAAGCA TCAGCAGAAG 
CTGGAACAGT GCCTGCAGCT GCGGCATTTT GAGCAAGGTT TCCGGGAGGT CAAAACCACC 
TTGGATTCCA TGTCCCAGAA GATAGCTGCT TTCACGGATG TTGGCAACAG CCTGGCCCAC 
GTGCAGCACC TTCTGAAGGA CTTGACCGCC TTTGAGGAGA AGTCCAGTGT GGCTGTGGAC 
AAGGCCCGCG CCCTGTCACT GGAAGGTCAG CAGCTGATTG AGAACAGGCA CTACGCTGTG 
GACTCCATCC ACCCCAAGTG TGAAGAGCTC CAGCACCTCT GTGACCACTT CGCCTCTGAG 
GTCACCAGGA GGCGGGGCCT GCTCAGCAAG TCCTTGGAGC TGCATAGCCT CCTGGAGACG 
TCCATGAAGT GGAGCGACGA GGGGATCTTC CTGCTGGCCT CGCAGCCTGT GGACAAATGC 
CAGTCCCAGG ATGGCGCAGA GGCGGCCCTC CAGGAGATTG AGAAGTTTCT GGAGACCGGT 
GCAGAAAATA AGATTCAGGA GCTCAATGAG ATTTACAAGG AATACGAATG CATCCTCAAC 
CAGGACCTTC TGGAACACGT GCAAAAAGTC TTTCAGAAGC AAGAAAGCAC GGAGGAGATG 
TTCCACCGCA GGCAAGCCAG CCTGAAGAAA CTGGCAGCCA AGCAGACACG GCCTGTGCAG 
CCCGTGGCCC CCCGACCAGA GGCGCTTACC AAGTCGCCCT CCCCCTCGCC AGGCTCCTGG 
CGAAGCTCTG AGAACTCCAG CTCTGAGGGC AACGCACTCC GCAGAGGGCC CTACAGGAGA 
GCCAAGAGCG AAATGAGTGA ACCCCGGCAG GGCCGCACCA GCTCCACAGG GGAAGAGGAA 
GAGAGCCTGG CCATCCTGCG CAGACACGTG ATGAATGAGC TTCTGGACAC CGAGCGGGCC 
TACGTGGAGG AGCTGCTCTG TGTCTTAGAG GGCTATGCCG CAGAGATGGA TAACCCCTTG 
ATGGCACACC TCATCTCAAC AGGCCTGCAA AACAAGAAGA ACATTCTGTT TGGAAACATG 
GAGGAAATCT ATCATTTTCA CAACAGAATA TTCCTGCGGG AGCTGGAGAG CTGCATAGAC 
TGCCCAGAGC TGGTGGGGAG GTGCTTTCTG GAAAGGATGG AGGAGTTCCA GATCTATGAG 
AAGTACTGTC AGAACAAGCC ACGCTCTGAG AGCCTGTGGA GGCAGTGCTC TGACTGCCCC 
TTCTTCCAGG AGTGTCAGAA GAAGCTGGAC CACAAGCTGA GCCTGGACTC CTACCTGCTG 
AAACCTGTGC AGAGGATAAC CAAGTACCAG CTGCTGCTCA AGGAAATGCT GAAGTACAGC 
AAGCACTGTG AAGGGGCAGA GGACCTGCAG GAGGCGCTGA GCTCCATCCT GGGCATCCTC 
AAGGCTGTGA ACGACTCCAT GCATCTCATC GCCATCACTG GCTATGATGG AAACCTTGGC 
GACCTGGGGA AGCTGCTGAT GCAAGGTTCC TTCAGCGTGT GGACAGACCA CAAGAAGGGC 
CACACCAAAG TGAAGGAGCT AGCCAGGTTC AAGCCCATGC AGCGGCACCT GTTTCTGCAC 
GAGAAGGCCG TCCTCTTCTG CAAGAAGAGG GAGGAGAATG GGGAGGGATA CGAGAAGGCT 
CCTTCCTACA GCTACAAGCA GTCCTTGAAT ATGACGGCTG TCGGCATCAC AGAGAACGTG 
AAGGGTGACA CCAAGAAGTT CGAGATCTGG TACAACGCAA GGGAGGAGGT TTATATCATT 
CAGGCGCCAA CCCCCGAGAT TAAAGCAGCG TGGGTGAATG AGATTCGGAA GGTGCTGACC 
AGCCAGCTGC AGGCTTGCCG GGAGGCCAGC CAGCACCGAG CCCTGGAGCA GTCCCACAGC 
CTTCCTTTGC CCACGCCATC CAGCACCAGT CCCACAAAAG GGAACACAAG AAATGTCAAG 
AAGCTCGAAG ACCGGAAGAC GGACCCGCTC AGCCTGGAAG GCTACGTGAG CTCATCGTTA 
CCAAAGCCCC CTGAGAAGGG CAAAGGCTGG AGCAAAACAT CCCACTCCCT GGAGGCCCCT 
GAGGAGGACG GAGGCTGGTC CAGTGCTGAG GAACTCATCA ACTCATCTGA TGCTGAGGAA 
GACGGTGGAG TGGGCCCTAA GAAGCTGGTG CCGGGTAAAT ACACAGTGGT GATGGATGAT 
GAGAAAGGGG GCCCTGACAC CCTTGCCATG AGGAGTGGAG ATATGGTGGA GGTGGTGGAA 
GAAGGCGCTG AGGGCCTCTG GTATGTTCGG GACCTGACCA GCAGCAAGGA GGGATGGGTG 
CCAGCCAGCA GCCTATCCAC CCTCCTGGGC AAATCCAGTT CAGCCCAGTG TCTGAGCAGC 
TCAGGTAAGA TCCACTGTGC GCGCCAGCTG TGCCCTGAGC CTGCCGAGAT CCTCAGCCCA 
GAGCCCGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( NM_001371047.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001357976.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OMu84909
Clone ID Related Accession (Same CDS sequence)	NM_001371047.1
Accession Version	NM_001371047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-24
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform 8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127308.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.2146617.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAGGCC 
CCTGAGGAGG ACGGAGGCTG GTCCAGTGCT GAGGAACTCA TCAACTCATC TGATGCTGAG 
GAAGACGGTG GAGTGGGCCC TAAGAAGCTG GTGCCGGGTA AATACACAGT GGTGATGGAT 
GATGAGAAAG GGGGCCCTGA CACCCTTGCC ATGAGGAGTG GAGATATGGT GGAGGTGGTG 
GAAGAAGGCG CTGAGGGCCT CTGGTATGTT CGGGACCTGA CCAGCAGCAA GGAGGGATGG 
GTGCCAGCCA GCAGCCTATC CACCCTCCTG GGCAAATCCA GTTCAGCCCA GTGTCTGAGC 
AGCTCAGGTA AGATCCACTG TGCGCGCCAG CTGTGCCCTG AGCCTGCCGA GATCCTCAGC 
CCAGAGCCCG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357976.1
CDS	66..3500
Translation	MRFWLRNEEMALEEMVQRLNAVSKNTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGKIHCARQLCPEPAEILSPEPV

Target ORF information:

RefSeq Version	NM_001371047.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371047.1

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAGGCC 
CCTGAGGAGG ACGGAGGCTG GTCCAGTGCT GAGGAACTCA TCAACTCATC TGATGCTGAG 
GAAGACGGTG GAGTGGGCCC TAAGAAGCTG GTGCCGGGTA AATACACAGT GGTGATGGAT 
GATGAGAAAG GGGGCCCTGA CACCCTTGCC ATGAGGAGTG GAGATATGGT GGAGGTGGTG 
GAAGAAGGCG CTGAGGGCCT CTGGTATGTT CGGGACCTGA CCAGCAGCAA GGAGGGATGG 
GTGCCAGCCA GCAGCCTATC CACCCTCCTG GGCAAATCCA GTTCAGCCCA GTGTCTGAGC 
AGCTCAGGTA AGATCCACTG TGCGCGCCAG CTGTGCCCTG AGCCTGCCGA GATCCTCAGC 
CCAGAGCCCG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( NM_001371046.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001357975.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 7, mRNA.

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CloneID	OMu84911
Clone ID Related Accession (Same CDS sequence)	NM_001371046.1
Accession Version	NM_001371046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-24
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform 7
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127308.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR6116558.15509.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACCAGG ACATCGTCCC GCTCTGTGCC GCTGACATCC AGGAGCAGCT GAAGAAGCGC 
TTTGCTTACC TGTCTGGTGG GCGGGGACAG GACGGAAGCC CAGTCATCAC CTTCCCTGAC 
TACCCAGCCT TCAGCGAGAT CCCGGACAAG GAGTTCCAGA ATGTCATGAC CTATCTCACC 
AGCATCCCCA GTTTGCAAGA TGCTGGCATT GGATTCATCT TGGTCATAGA CCGAAGGCAG 
GACAAATGGA CCTCTGTGAA GGCATCCGTC CTGCGGATAG CCGCATCTTT CCCAGCCAAC 
CTGCAGCTTG TCCTTGTTCT TCGGCCCACG GGCTTTTTCC AGAGGACTCT CTCGGACATT 
GCCTTCAAGT TCAACAGAGA TGAGTTTAAG ATGAAAGTGC CGGTCATGAT GCTAAGCTCA 
GTGCCAGAAC TACACGGCTA CATTGATAAG TCGCAGCTGA CCGAGGACCT CGGGGGGACC 
CTGGACTACT GCCACTCCAG GTGGCTGTGC CACCGCACAG CAATTGAAAG CTTCGCCCTG 
ATGGTGAAGC AGACAGCCCA GATGCTGCAG GCCTTTGGGA CCGAGCTGGC TGAAACAGAG 
CTGCCGAATG ACGTCCAGTC AACCAGCCTG GTGCTCAGCG CACACACAGA GAAGAAGGCC 
AAAGTGAAGG AAGATCTGCA GTTAGCACTG AAGGAGGGGA ACAGCATCCT GGAGAGCCTC 
AGAGAGCCAC TGGCTGAGAG CGCGGCCCAC AGTGTGAACC AGGACCAGCT GGACAATCAG 
GCCACCGTGC AGAGGCTCCT GGCCCAGCTG AATGAGACGG AGGCTGCCTT TGACGAATTC 
TGGGCAAAGC ATCAGCAGAA GCTGGAACAG TGCCTGCAGC TGCGGCATTT TGAGCAAGGT 
TTCCGGGAGG TCAAAACCAC CTTGGATTCC ATGTCCCAGA AGATAGCTGC TTTCACGGAT 
GTTGGCAACA GCCTGGCCCA CGTGCAGCAC CTTCTGAAGG ACTTGACCGC CTTTGAGGAG 
AAGTCCAGTG TGGCTGTGGA CAAGGCCCGC GCCCTGTCAC TGGAAGGTCA GCAGCTGATT 
GAGAACAGGC ACTACGCTGT GGACTCCATC CACCCCAAGT GTGAAGAGCT CCAGCACCTC 
TGTGACCACT TCGCCTCTGA GGTCACCAGG AGGCGGGGCC TGCTCAGCAA GTCCTTGGAG 
CTGCATAGCC TCCTGGAGAC GTCCATGAAG TGGAGCGACG AGGGGATCTT CCTGCTGGCC 
TCGCAGCCTG TGGACAAATG CCAGTCCCAG GATGGCGCAG AGGCGGCCCT CCAGGAGATT 
GAGAAGTTTC TGGAGACCGG TGCAGAAAAT AAGATTCAGG AGCTCAATGA GATTTACAAG 
GAATACGAAT GCATCCTCAA CCAGGACCTT CTGGAACACG TGCAAAAAGT CTTTCAGAAG 
CAAGAAAGCA CGGAGGAGAT GTTCCACCGC AGGCAAGCCA GCCTGAAGAA ACTGGCAGCC 
AAGCAGACAC GGCCTGTGCA GCCCGTGGCC CCCCGACCAG AGGCGCTTAC CAAGTCGCCC 
TCCCCCTCGC CAGGCTCCTG GCGAAGCTCT GAGAACTCCA GCTCTGAGGG CAACGCACTC 
CGCAGAGGGC CCTACAGGAG AGCCAAGAGC GAAATGAGTG AACCCCGGCA GGGCCGCACC 
AGCTCCACAG GGGAAGAGGA AGAGAGCCTG GCCATCCTGC GCAGACACGT GATGAATGAG 
CTTCTGGACA CCGAGCGGGC CTACGTGGAG GAGCTGCTCT GTGTCTTAGA GGGCTATGCC 
GCAGAGATGG ATAACCCCTT GATGGCACAC CTCATCTCAA CAGGCCTGCA AAACAAGAAG 
AACATTCTGT TTGGAAACAT GGAGGAAATC TATCATTTTC ACAACAGAAT ATTCCTGCGG 
GAGCTGGAGA GCTGCATAGA CTGCCCAGAG CTGGTGGGGA GGTGCTTTCT GGAAAGGATG 
GAGGAGTTCC AGATCTATGA GAAGTACTGT CAGAACAAGC CACGCTCTGA GAGCCTGTGG 
AGGCAGTGCT CTGACTGCCC CTTCTTCCAG GAGTGTCAGA AGAAGCTGGA CCACAAGCTG 
AGCCTGGACT CCTACCTGCT GAAACCTGTG CAGAGGATAA CCAAGTACCA GCTGCTGCTC 
AAGGAAATGC TGAAGTACAG CAAGCACTGT GAAGGGGCAG AGGACCTGCA GGAGGCGCTG 
AGCTCCATCC TGGGCATCCT CAAGGCTGTG AACGACTCCA TGCATCTCAT CGCCATCACT 
GGCTATGATG GAAACCTTGG CGACCTGGGG AAGCTGCTGA TGCAAGGTTC CTTCAGCGTG 
TGGACAGACC ACAAGAAGGG CCACACCAAA GTGAAGGAGC TAGCCAGGTT CAAGCCCATG 
CAGCGGCACC TGTTTCTGCA CGAGAAGGCC GTCCTCTTCT GCAAGAAGAG GGAGGAGAAT 
GGGGAGGGAT ACGAGAAGGC TCCTTCCTAC AGCTACAAGC AGTCCTTGAA TATGACGGCT 
GTCGGCATCA CAGAGAACGT GAAGGGTGAC ACCAAGAAGT TCGAGATCTG GTACAACGCA 
AGGGAGGAGG TTTATATCAT TCAGGCGCCA ACCCCCGAGA TTAAAGCAGC GTGGGTGAAT 
GAGATTCGGA AGGTGCTGAC CAGCCAGCTG CAGGCTTGCC GGGAGGCCAG CCAGCACCGA 
GCCCTGGAGC AGTCCCACAG CCTTCCTTTG CCCACGCCAT CCAGCACCAG TCCCACAAAA 
GGGAACACAA GAAATGTCAA GAAGCTCGAA GACCGGAAGA CGGACCCGCT CAGCCTGGAA 
GGCTACGTGA GCTCATCGTT ACCAAAGCCC CCTGAGAAGG GCAAAGCCTC TCCGACCAGT 
CCTGACAAAA AAGCTAAGCG GCACGAAGTA AAGAGCGACC CGACTCCCTT TGGTTTGCGA 
GGCTGGAGCA AAACATCCCA CTCCCTGGAG GCCCCTGAGG AGGACGGAGG CTGGTCCAGT 
GCTGAGGAAC TCATCAACTC ATCTGATGCT GAGGAAGACG GTGGAGTGGG CCCTAAGAAG 
CTGGTGCCGG GTAAATACAC AGTGGTGATG GATGATGAGA AAGGGGGCCC TGACACCCTT 
GCCATGAGGA GTGGAGATAT GGTGGAGGTG GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT 
GTTCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT ATCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC 
GTGCTGAGCA GCTCATCCAG CTTCAGCGAG GGCTACCCTG CTCCCTTCTC CGAGCTCCAG 
GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357975.1
CDS	267..3632
Translation	MHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	NM_001371046.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371046.1

ORF Insert Sequence:

ATGCACCAGG ACATCGTCCC GCTCTGTGCC GCTGACATCC AGGAGCAGCT GAAGAAGCGC 
TTTGCTTACC TGTCTGGTGG GCGGGGACAG GACGGAAGCC CAGTCATCAC CTTCCCTGAC 
TACCCAGCCT TCAGCGAGAT CCCGGACAAG GAGTTCCAGA ATGTCATGAC CTATCTCACC 
AGCATCCCCA GTTTGCAAGA TGCTGGCATT GGATTCATCT TGGTCATAGA CCGAAGGCAG 
GACAAATGGA CCTCTGTGAA GGCATCCGTC CTGCGGATAG CCGCATCTTT CCCAGCCAAC 
CTGCAGCTTG TCCTTGTTCT TCGGCCCACG GGCTTTTTCC AGAGGACTCT CTCGGACATT 
GCCTTCAAGT TCAACAGAGA TGAGTTTAAG ATGAAAGTGC CGGTCATGAT GCTAAGCTCA 
GTGCCAGAAC TACACGGCTA CATTGATAAG TCGCAGCTGA CCGAGGACCT CGGGGGGACC 
CTGGACTACT GCCACTCCAG GTGGCTGTGC CACCGCACAG CAATTGAAAG CTTCGCCCTG 
ATGGTGAAGC AGACAGCCCA GATGCTGCAG GCCTTTGGGA CCGAGCTGGC TGAAACAGAG 
CTGCCGAATG ACGTCCAGTC AACCAGCCTG GTGCTCAGCG CACACACAGA GAAGAAGGCC 
AAAGTGAAGG AAGATCTGCA GTTAGCACTG AAGGAGGGGA ACAGCATCCT GGAGAGCCTC 
AGAGAGCCAC TGGCTGAGAG CGCGGCCCAC AGTGTGAACC AGGACCAGCT GGACAATCAG 
GCCACCGTGC AGAGGCTCCT GGCCCAGCTG AATGAGACGG AGGCTGCCTT TGACGAATTC 
TGGGCAAAGC ATCAGCAGAA GCTGGAACAG TGCCTGCAGC TGCGGCATTT TGAGCAAGGT 
TTCCGGGAGG TCAAAACCAC CTTGGATTCC ATGTCCCAGA AGATAGCTGC TTTCACGGAT 
GTTGGCAACA GCCTGGCCCA CGTGCAGCAC CTTCTGAAGG ACTTGACCGC CTTTGAGGAG 
AAGTCCAGTG TGGCTGTGGA CAAGGCCCGC GCCCTGTCAC TGGAAGGTCA GCAGCTGATT 
GAGAACAGGC ACTACGCTGT GGACTCCATC CACCCCAAGT GTGAAGAGCT CCAGCACCTC 
TGTGACCACT TCGCCTCTGA GGTCACCAGG AGGCGGGGCC TGCTCAGCAA GTCCTTGGAG 
CTGCATAGCC TCCTGGAGAC GTCCATGAAG TGGAGCGACG AGGGGATCTT CCTGCTGGCC 
TCGCAGCCTG TGGACAAATG CCAGTCCCAG GATGGCGCAG AGGCGGCCCT CCAGGAGATT 
GAGAAGTTTC TGGAGACCGG TGCAGAAAAT AAGATTCAGG AGCTCAATGA GATTTACAAG 
GAATACGAAT GCATCCTCAA CCAGGACCTT CTGGAACACG TGCAAAAAGT CTTTCAGAAG 
CAAGAAAGCA CGGAGGAGAT GTTCCACCGC AGGCAAGCCA GCCTGAAGAA ACTGGCAGCC 
AAGCAGACAC GGCCTGTGCA GCCCGTGGCC CCCCGACCAG AGGCGCTTAC CAAGTCGCCC 
TCCCCCTCGC CAGGCTCCTG GCGAAGCTCT GAGAACTCCA GCTCTGAGGG CAACGCACTC 
CGCAGAGGGC CCTACAGGAG AGCCAAGAGC GAAATGAGTG AACCCCGGCA GGGCCGCACC 
AGCTCCACAG GGGAAGAGGA AGAGAGCCTG GCCATCCTGC GCAGACACGT GATGAATGAG 
CTTCTGGACA CCGAGCGGGC CTACGTGGAG GAGCTGCTCT GTGTCTTAGA GGGCTATGCC 
GCAGAGATGG ATAACCCCTT GATGGCACAC CTCATCTCAA CAGGCCTGCA AAACAAGAAG 
AACATTCTGT TTGGAAACAT GGAGGAAATC TATCATTTTC ACAACAGAAT ATTCCTGCGG 
GAGCTGGAGA GCTGCATAGA CTGCCCAGAG CTGGTGGGGA GGTGCTTTCT GGAAAGGATG 
GAGGAGTTCC AGATCTATGA GAAGTACTGT CAGAACAAGC CACGCTCTGA GAGCCTGTGG 
AGGCAGTGCT CTGACTGCCC CTTCTTCCAG GAGTGTCAGA AGAAGCTGGA CCACAAGCTG 
AGCCTGGACT CCTACCTGCT GAAACCTGTG CAGAGGATAA CCAAGTACCA GCTGCTGCTC 
AAGGAAATGC TGAAGTACAG CAAGCACTGT GAAGGGGCAG AGGACCTGCA GGAGGCGCTG 
AGCTCCATCC TGGGCATCCT CAAGGCTGTG AACGACTCCA TGCATCTCAT CGCCATCACT 
GGCTATGATG GAAACCTTGG CGACCTGGGG AAGCTGCTGA TGCAAGGTTC CTTCAGCGTG 
TGGACAGACC ACAAGAAGGG CCACACCAAA GTGAAGGAGC TAGCCAGGTT CAAGCCCATG 
CAGCGGCACC TGTTTCTGCA CGAGAAGGCC GTCCTCTTCT GCAAGAAGAG GGAGGAGAAT 
GGGGAGGGAT ACGAGAAGGC TCCTTCCTAC AGCTACAAGC AGTCCTTGAA TATGACGGCT 
GTCGGCATCA CAGAGAACGT GAAGGGTGAC ACCAAGAAGT TCGAGATCTG GTACAACGCA 
AGGGAGGAGG TTTATATCAT TCAGGCGCCA ACCCCCGAGA TTAAAGCAGC GTGGGTGAAT 
GAGATTCGGA AGGTGCTGAC CAGCCAGCTG CAGGCTTGCC GGGAGGCCAG CCAGCACCGA 
GCCCTGGAGC AGTCCCACAG CCTTCCTTTG CCCACGCCAT CCAGCACCAG TCCCACAAAA 
GGGAACACAA GAAATGTCAA GAAGCTCGAA GACCGGAAGA CGGACCCGCT CAGCCTGGAA 
GGCTACGTGA GCTCATCGTT ACCAAAGCCC CCTGAGAAGG GCAAAGCCTC TCCGACCAGT 
CCTGACAAAA AAGCTAAGCG GCACGAAGTA AAGAGCGACC CGACTCCCTT TGGTTTGCGA 
GGCTGGAGCA AAACATCCCA CTCCCTGGAG GCCCCTGAGG AGGACGGAGG CTGGTCCAGT 
GCTGAGGAAC TCATCAACTC ATCTGATGCT GAGGAAGACG GTGGAGTGGG CCCTAAGAAG 
CTGGTGCCGG GTAAATACAC AGTGGTGATG GATGATGAGA AAGGGGGCCC TGACACCCTT 
GCCATGAGGA GTGGAGATAT GGTGGAGGTG GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT 
GTTCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT ATCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC 
GTGCTGAGCA GCTCATCCAG CTTCAGCGAG GGCTACCCTG CTCCCTTCTC CGAGCTCCAG 
GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( NM_001371049.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001357978.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 10, mRNA.

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CloneID	OMu84912
Clone ID Related Accession (Same CDS sequence)	NM_001371049.1
Accession Version	NM_001371049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-24
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform 10
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127308.3. Transcript Variant: This variant (10) encodes the longest isoform (10). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7652917.1018109.1, SRR7345562.1984838.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGACGATGC GGTCCCTAGT TCTACCTCTG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC CTCAAAGCCT CTCCGACCAG TCCTGACAAA 
AAAGCTAAGC GGCACGAAGT AAAGAGCGAC CCGACTCCCT TTGGTTTGCG AGGCTGGAGC 
AAAACATCCC ACTCCCTGGA GGCCCCTGAG GAGGACGGAG GCTGGTCCAG TGCTGAGGAA 
CTCATCAACT CATCTGATGC TGAGGAAGAC GGTGGAGTGG GCCCTAAGAA GCTGGTGCCG 
GGTAAATACA CAGTGGTGAT GGATGATGAG AAAGGGGGCC CTGACACCCT TGCCATGAGG 
AGTGGAGATA TGGTGGAGGT GGTGGAAGAA GGCGCTGAGG GCCTCTGGTA TGTTCGGGAC 
CTGACCAGCA GCAAGGAGGG ATGGGTGCCA GCCAGCAGCC TATCCACCCT CCTGGGCAAA 
TCCAGTTCAG CCCAGTGTCT GAGCAGCTCA GACTCCAGCC CAGGGTCAGC CGTGCTGAGC 
AGCTCATCCA GCTTCAGCGA GGGCTACCCT GCTCCCTTCT CCGAGCTCCA GGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357978.1
CDS	66..3602
Translation	MRFWLRNEEMALEEMVQRLNAVSKNTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	NM_001371049.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371049.1

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGACGATGC GGTCCCTAGT TCTACCTCTG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC CTCAAAGCCT CTCCGACCAG TCCTGACAAA 
AAAGCTAAGC GGCACGAAGT AAAGAGCGAC CCGACTCCCT TTGGTTTGCG AGGCTGGAGC 
AAAACATCCC ACTCCCTGGA GGCCCCTGAG GAGGACGGAG GCTGGTCCAG TGCTGAGGAA 
CTCATCAACT CATCTGATGC TGAGGAAGAC GGTGGAGTGG GCCCTAAGAA GCTGGTGCCG 
GGTAAATACA CAGTGGTGAT GGATGATGAG AAAGGGGGCC CTGACACCCT TGCCATGAGG 
AGTGGAGATA TGGTGGAGGT GGTGGAAGAA GGCGCTGAGG GCCTCTGGTA TGTTCGGGAC 
CTGACCAGCA GCAAGGAGGG ATGGGTGCCA GCCAGCAGCC TATCCACCCT CCTGGGCAAA 
TCCAGTTCAG CCCAGTGTCT GAGCAGCTCA GACTCCAGCC CAGGGTCAGC CGTGCTGAGC 
AGCTCATCCA GCTTCAGCGA GGGCTACCCT GCTCCCTTCT CCGAGCTCCA GGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_017312595.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168084.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X16, mRNA.

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CloneID	OMu82370
Clone ID Related Accession (Same CDS sequence)	XM_017312595.1 , NM_001371044.1
Accession Version	XM_017312595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAGGCC 
CCTGAGGAGG ACGGAGGCTG GTCCAGTGCT GAGGAACTCA TCAACTCATC TGATGCTGAG 
GAAGACGGTG GAGTGGGCCC TAAGAAGCTG GTGCCGGGTA AATACACAGT GGTGATGGAT 
GATGAGAAAG GGGGCCCTGA CACCCTTGCC ATGAGGAGTG GAGATATGGT GGAGGTGGTG 
GAAGAAGGCG CTGAGGGCCT CTGGTATGTT CGGGACCTGA CCAGCAGCAA GGAGGGATGG 
GTGCCAGCCA GCAGCCTATC CACCCTCCTG GGCAAATCCA GTTCAGCCCA GTGTCTGAGC 
AGCTCAGACT CCAGCCCAGG GTCAGCCGTG CTGAGCAGCT CATCCAGCTT CAGCGAGGGC 
TACCCTGCTC CCTTCTCCGA GCTCCAGGGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168084.1
CDS	100..3552
Translation	MRFWLRNEEMALEEMVQRLNAVSKNTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	XM_017312595.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312595.1

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAGGCC 
CCTGAGGAGG ACGGAGGCTG GTCCAGTGCT GAGGAACTCA TCAACTCATC TGATGCTGAG 
GAAGACGGTG GAGTGGGCCC TAAGAAGCTG GTGCCGGGTA AATACACAGT GGTGATGGAT 
GATGAGAAAG GGGGCCCTGA CACCCTTGCC ATGAGGAGTG GAGATATGGT GGAGGTGGTG 
GAAGAAGGCG CTGAGGGCCT CTGGTATGTT CGGGACCTGA CCAGCAGCAA GGAGGGATGG 
GTGCCAGCCA GCAGCCTATC CACCCTCCTG GGCAAATCCA GTTCAGCCCA GTGTCTGAGC 
AGCTCAGACT CCAGCCCAGG GTCAGCCGTG CTGAGCAGCT CATCCAGCTT CAGCGAGGGC 
TACCCTGCTC CCTTCTCCGA GCTCCAGGGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( NM_001159485.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001152957.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 3, mRNA.

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CloneID	OMu07468
Clone ID Related Accession (Same CDS sequence)	NM_001159485.1 , NM_001159485.2
Accession Version	NM_001159485.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY202611.1, BC058622.1, CF618259.1 and AI853669.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' and 3' coding regions, compared to variant 1, resulting in an isoform (3) that has distinct N- and C-termini and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK170558.1, DQ978380.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGACCCA CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC CGCTGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGACA GGACGGAAGC 
CCAGTCATCA CCTTCCCTGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATC 
TTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCTGTGA AGGCATCCGT CCTGCGGATA 
GCCGCATCTT TCCCAGCCAA CCTGCAGCTT GTCCTTGTTC TTCGGCCCAC GGGCTTTTTC 
CAGAGGACTC TCTCGGACAT TGCCTTCAAG TTCAACAGAG ATGAGTTTAA GATGAAAGTG 
CCGGTCATGA TGCTAAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCGCAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCACTCCA GGTGGCTGTG CCACCGCACA 
GCAATTGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCCC AGATGCTGCA GGCCTTTGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCGAAT GACGTCCAGT CAACCAGCCT GGTGCTCAGC 
GCACACACAG AGAAGAAGGC CAAAGTGAAG GAAGATCTGC AGTTAGCACT GAAGGAGGGG 
AACAGCATCC TGGAGAGCCT CAGAGAGCCA CTGGCTGAGA GCGCGGCCCA CAGTGTGAAC 
CAGGACCAGC TGGACAATCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAGACG 
GAGGCTGCCT TTGACGAATT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAAGG TTTCCGGGAG GTCAAAACCA CCTTGGATTC CATGTCCCAG 
AAGATAGCTG CTTTCACGGA TGTTGGCAAC AGCCTGGCCC ACGTGCAGCA CCTTCTGAAG 
GACTTGACCG CCTTTGAGGA GAAGTCCAGT GTGGCTGTGG ACAAGGCCCG CGCCCTGTCA 
CTGGAAGGTC AGCAGCTGAT TGAGAACAGG CACTACGCTG TGGACTCCAT CCACCCCAAG 
TGTGAAGAGC TCCAGCACCT CTGTGACCAC TTCGCCTCTG AGGTCACCAG GAGGCGGGGC 
CTGCTCAGCA AGTCCTTGGA GCTGCATAGC CTCCTGGAGA CGTCCATGAA GTGGAGCGAC 
GAGGGGATCT TCCTGCTGGC CTCGCAGCCT GTGGACAAAT GCCAGTCCCA GGATGGCGCA 
GAGGCGGCCC TCCAGGAGAT TGAGAAGTTT CTGGAGACCG GTGCAGAAAA TAAGATTCAG 
GAGCTCAATG AGATTTACAA GGAATACGAA TGCATCCTCA ACCAGGACCT TCTGGAACAC 
GTGCAAAAAG TCTTTCAGAA GCAAGAAAGC ACGGAGGAGA TGTTCCACCG CAGGCAAGCC 
AGCCTGAAGA AACTGGCAGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCCCGACCA 
GAGGCGCTTA CCAAGTCGCC CTCCCCCTCG CCAGGCTCCT GGCGAAGCTC TGAGAACTCC 
AGCTCTGAGG GCAACGCACT CCGCAGAGGG CCCTACAGGA GAGCCAAGAG CGAAATGAGT 
GAACCCCGGC AGGGCCGCAC CAGCTCCACA GGGGAAGAGG AAGAGAGCCT GGCCATCCTG 
CGCAGACACG TGATGAATGA GCTTCTGGAC ACCGAGCGGG CCTACGTGGA GGAGCTGCTC 
TGTGTCTTAG AGGGCTATGC CGCAGAGATG GATAACCCCT TGATGGCACA CCTCATCTCA 
ACAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAAAT CTATCATTTT 
CACAACAGAA TATTCCTGCG GGAGCTGGAG AGCTGCATAG ACTGCCCAGA GCTGGTGGGG 
AGGTGCTTTC TGGAAAGGAT GGAGGAGTTC CAGATCTATG AGAAGTACTG TCAGAACAAG 
CCACGCTCTG AGAGCCTGTG GAGGCAGTGC TCTGACTGCC CCTTCTTCCA GGAGTGTCAG 
AAGAAGCTGG ACCACAAGCT GAGCCTGGAC TCCTACCTGC TGAAACCTGT GCAGAGGATA 
ACCAAGTACC AGCTGCTGCT CAAGGAAATG CTGAAGTACA GCAAGCACTG TGAAGGGGCA 
GAGGACCTGC AGGAGGCGCT GAGCTCCATC CTGGGCATCC TCAAGGCTGT GAACGACTCC 
ATGCATCTCA TCGCCATCAC TGGCTATGAT GGAAACCTTG GCGACCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGCGT GTGGACAGAC CACAAGAAGG GCCACACCAA AGTGAAGGAG 
CTAGCCAGGT TCAAGCCCAT GCAGCGGCAC CTGTTTCTGC ACGAGAAGGC CGTCCTCTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGA TACGAGAAGG CTCCTTCCTA CAGCTACAAG 
CAGTCCTTGA ATATGACGGC TGTCGGCATC ACAGAGAACG TGAAGGGTGA CACCAAGAAG 
TTCGAGATCT GGTACAACGC AAGGGAGGAG GTTTATATCA TTCAGGCGCC AACCCCCGAG 
ATTAAAGCAG CGTGGGTGAA TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCTTGC 
CGGGAGGCCA GCCAGCACCG AGCCCTGGAG CAGTCCCACA GCCTTCCTTT GCCCACGCCA 
TCCAGCACCA GTCCCACAAA AGGGAACACA AGAAATGTCA AGAAGCTCGA AGACCGGAAG 
ACGGACCCGC TCAGCCTGGA AGGCTACGTG AGCTCATCGT TACCAAAGCC CCCTGAGAAG 
GGCAAAGGCT GGAGCAAAAC ATCCCACTCC CTGGAGGCCC CTGAGGAGGA CGGAGGCTGG 
TCCAGTGCTG AGGAACTCAT CAACTCATCT GATGCTGAGG AAGACGGTGG AGTGGGCCCT 
AAGAAGCTGG TGCCGGGTAA ATACACAGTG GTGATGGATG ATGAGAAAGG GGGCCCTGAC 
ACCCTTGCCA TGAGGAGTGG AGATATGGTG GAGGTGGTGG AAGAAGGCGC TGAGGGCCTC 
TGGTATGTTC GGGACCTGAC CAGCAGCAAG GAGGGATGGG TGCCAGCCAG CAGCCTATCC 
ACCCTCCTGG GCAAATCCAG TTCAGCCCAG TGTCTGAGCA GCTCAGGTAA GATCCACTGT 
GCGCGCCAGC TGTGCCCTGA GCCTGCCGAG ATCCTCAGCC CAGAGCCCGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001152957.1
CDS	247..3540
Translation	MRPTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGKIHCARQLCPEPAEILSPEPV

Target ORF information:

RefSeq Version	NM_001159485.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159485.1

ORF Insert Sequence:

ATGAGACCCA CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC CGCTGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGACA GGACGGAAGC 
CCAGTCATCA CCTTCCCTGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATC 
TTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCTGTGA AGGCATCCGT CCTGCGGATA 
GCCGCATCTT TCCCAGCCAA CCTGCAGCTT GTCCTTGTTC TTCGGCCCAC GGGCTTTTTC 
CAGAGGACTC TCTCGGACAT TGCCTTCAAG TTCAACAGAG ATGAGTTTAA GATGAAAGTG 
CCGGTCATGA TGCTAAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCGCAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCACTCCA GGTGGCTGTG CCACCGCACA 
GCAATTGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCCC AGATGCTGCA GGCCTTTGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCGAAT GACGTCCAGT CAACCAGCCT GGTGCTCAGC 
GCACACACAG AGAAGAAGGC CAAAGTGAAG GAAGATCTGC AGTTAGCACT GAAGGAGGGG 
AACAGCATCC TGGAGAGCCT CAGAGAGCCA CTGGCTGAGA GCGCGGCCCA CAGTGTGAAC 
CAGGACCAGC TGGACAATCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAGACG 
GAGGCTGCCT TTGACGAATT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAAGG TTTCCGGGAG GTCAAAACCA CCTTGGATTC CATGTCCCAG 
AAGATAGCTG CTTTCACGGA TGTTGGCAAC AGCCTGGCCC ACGTGCAGCA CCTTCTGAAG 
GACTTGACCG CCTTTGAGGA GAAGTCCAGT GTGGCTGTGG ACAAGGCCCG CGCCCTGTCA 
CTGGAAGGTC AGCAGCTGAT TGAGAACAGG CACTACGCTG TGGACTCCAT CCACCCCAAG 
TGTGAAGAGC TCCAGCACCT CTGTGACCAC TTCGCCTCTG AGGTCACCAG GAGGCGGGGC 
CTGCTCAGCA AGTCCTTGGA GCTGCATAGC CTCCTGGAGA CGTCCATGAA GTGGAGCGAC 
GAGGGGATCT TCCTGCTGGC CTCGCAGCCT GTGGACAAAT GCCAGTCCCA GGATGGCGCA 
GAGGCGGCCC TCCAGGAGAT TGAGAAGTTT CTGGAGACCG GTGCAGAAAA TAAGATTCAG 
GAGCTCAATG AGATTTACAA GGAATACGAA TGCATCCTCA ACCAGGACCT TCTGGAACAC 
GTGCAAAAAG TCTTTCAGAA GCAAGAAAGC ACGGAGGAGA TGTTCCACCG CAGGCAAGCC 
AGCCTGAAGA AACTGGCAGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCCCGACCA 
GAGGCGCTTA CCAAGTCGCC CTCCCCCTCG CCAGGCTCCT GGCGAAGCTC TGAGAACTCC 
AGCTCTGAGG GCAACGCACT CCGCAGAGGG CCCTACAGGA GAGCCAAGAG CGAAATGAGT 
GAACCCCGGC AGGGCCGCAC CAGCTCCACA GGGGAAGAGG AAGAGAGCCT GGCCATCCTG 
CGCAGACACG TGATGAATGA GCTTCTGGAC ACCGAGCGGG CCTACGTGGA GGAGCTGCTC 
TGTGTCTTAG AGGGCTATGC CGCAGAGATG GATAACCCCT TGATGGCACA CCTCATCTCA 
ACAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAAAT CTATCATTTT 
CACAACAGAA TATTCCTGCG GGAGCTGGAG AGCTGCATAG ACTGCCCAGA GCTGGTGGGG 
AGGTGCTTTC TGGAAAGGAT GGAGGAGTTC CAGATCTATG AGAAGTACTG TCAGAACAAG 
CCACGCTCTG AGAGCCTGTG GAGGCAGTGC TCTGACTGCC CCTTCTTCCA GGAGTGTCAG 
AAGAAGCTGG ACCACAAGCT GAGCCTGGAC TCCTACCTGC TGAAACCTGT GCAGAGGATA 
ACCAAGTACC AGCTGCTGCT CAAGGAAATG CTGAAGTACA GCAAGCACTG TGAAGGGGCA 
GAGGACCTGC AGGAGGCGCT GAGCTCCATC CTGGGCATCC TCAAGGCTGT GAACGACTCC 
ATGCATCTCA TCGCCATCAC TGGCTATGAT GGAAACCTTG GCGACCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGCGT GTGGACAGAC CACAAGAAGG GCCACACCAA AGTGAAGGAG 
CTAGCCAGGT TCAAGCCCAT GCAGCGGCAC CTGTTTCTGC ACGAGAAGGC CGTCCTCTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGA TACGAGAAGG CTCCTTCCTA CAGCTACAAG 
CAGTCCTTGA ATATGACGGC TGTCGGCATC ACAGAGAACG TGAAGGGTGA CACCAAGAAG 
TTCGAGATCT GGTACAACGC AAGGGAGGAG GTTTATATCA TTCAGGCGCC AACCCCCGAG 
ATTAAAGCAG CGTGGGTGAA TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCTTGC 
CGGGAGGCCA GCCAGCACCG AGCCCTGGAG CAGTCCCACA GCCTTCCTTT GCCCACGCCA 
TCCAGCACCA GTCCCACAAA AGGGAACACA AGAAATGTCA AGAAGCTCGA AGACCGGAAG 
ACGGACCCGC TCAGCCTGGA AGGCTACGTG AGCTCATCGT TACCAAAGCC CCCTGAGAAG 
GGCAAAGGCT GGAGCAAAAC ATCCCACTCC CTGGAGGCCC CTGAGGAGGA CGGAGGCTGG 
TCCAGTGCTG AGGAACTCAT CAACTCATCT GATGCTGAGG AAGACGGTGG AGTGGGCCCT 
AAGAAGCTGG TGCCGGGTAA ATACACAGTG GTGATGGATG ATGAGAAAGG GGGCCCTGAC 
ACCCTTGCCA TGAGGAGTGG AGATATGGTG GAGGTGGTGG AAGAAGGCGC TGAGGGCCTC 
TGGTATGTTC GGGACCTGAC CAGCAGCAAG GAGGGATGGG TGCCAGCCAG CAGCCTATCC 
ACCCTCCTGG GCAAATCCAG TTCAGCCCAG TGTCTGAGCA GCTCAGGTAA GATCCACTGT 
GCGCGCCAGC TGTGCCCTGA GCCTGCCGAG ATCCTCAGCC CAGAGCCCGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( NM_001159485.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001152957.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu07468
Clone ID Related Accession (Same CDS sequence)	NM_001159485.1 , NM_001159485.2
Accession Version	NM_001159485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-24
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127308.3. On Jun 25, 2019 this sequence version replaced NM_001159485.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' and 3' coding regions, compared to variant 1, resulting in an isoform (3) that has distinct N- and C-termini and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK170558.1, DQ978380.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAGACCCA CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC CGCTGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGACA GGACGGAAGC 
CCAGTCATCA CCTTCCCTGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATC 
TTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCTGTGA AGGCATCCGT CCTGCGGATA 
GCCGCATCTT TCCCAGCCAA CCTGCAGCTT GTCCTTGTTC TTCGGCCCAC GGGCTTTTTC 
CAGAGGACTC TCTCGGACAT TGCCTTCAAG TTCAACAGAG ATGAGTTTAA GATGAAAGTG 
CCGGTCATGA TGCTAAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCGCAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCACTCCA GGTGGCTGTG CCACCGCACA 
GCAATTGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCCC AGATGCTGCA GGCCTTTGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCGAAT GACGTCCAGT CAACCAGCCT GGTGCTCAGC 
GCACACACAG AGAAGAAGGC CAAAGTGAAG GAAGATCTGC AGTTAGCACT GAAGGAGGGG 
AACAGCATCC TGGAGAGCCT CAGAGAGCCA CTGGCTGAGA GCGCGGCCCA CAGTGTGAAC 
CAGGACCAGC TGGACAATCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAGACG 
GAGGCTGCCT TTGACGAATT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAAGG TTTCCGGGAG GTCAAAACCA CCTTGGATTC CATGTCCCAG 
AAGATAGCTG CTTTCACGGA TGTTGGCAAC AGCCTGGCCC ACGTGCAGCA CCTTCTGAAG 
GACTTGACCG CCTTTGAGGA GAAGTCCAGT GTGGCTGTGG ACAAGGCCCG CGCCCTGTCA 
CTGGAAGGTC AGCAGCTGAT TGAGAACAGG CACTACGCTG TGGACTCCAT CCACCCCAAG 
TGTGAAGAGC TCCAGCACCT CTGTGACCAC TTCGCCTCTG AGGTCACCAG GAGGCGGGGC 
CTGCTCAGCA AGTCCTTGGA GCTGCATAGC CTCCTGGAGA CGTCCATGAA GTGGAGCGAC 
GAGGGGATCT TCCTGCTGGC CTCGCAGCCT GTGGACAAAT GCCAGTCCCA GGATGGCGCA 
GAGGCGGCCC TCCAGGAGAT TGAGAAGTTT CTGGAGACCG GTGCAGAAAA TAAGATTCAG 
GAGCTCAATG AGATTTACAA GGAATACGAA TGCATCCTCA ACCAGGACCT TCTGGAACAC 
GTGCAAAAAG TCTTTCAGAA GCAAGAAAGC ACGGAGGAGA TGTTCCACCG CAGGCAAGCC 
AGCCTGAAGA AACTGGCAGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCCCGACCA 
GAGGCGCTTA CCAAGTCGCC CTCCCCCTCG CCAGGCTCCT GGCGAAGCTC TGAGAACTCC 
AGCTCTGAGG GCAACGCACT CCGCAGAGGG CCCTACAGGA GAGCCAAGAG CGAAATGAGT 
GAACCCCGGC AGGGCCGCAC CAGCTCCACA GGGGAAGAGG AAGAGAGCCT GGCCATCCTG 
CGCAGACACG TGATGAATGA GCTTCTGGAC ACCGAGCGGG CCTACGTGGA GGAGCTGCTC 
TGTGTCTTAG AGGGCTATGC CGCAGAGATG GATAACCCCT TGATGGCACA CCTCATCTCA 
ACAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAAAT CTATCATTTT 
CACAACAGAA TATTCCTGCG GGAGCTGGAG AGCTGCATAG ACTGCCCAGA GCTGGTGGGG 
AGGTGCTTTC TGGAAAGGAT GGAGGAGTTC CAGATCTATG AGAAGTACTG TCAGAACAAG 
CCACGCTCTG AGAGCCTGTG GAGGCAGTGC TCTGACTGCC CCTTCTTCCA GGAGTGTCAG 
AAGAAGCTGG ACCACAAGCT GAGCCTGGAC TCCTACCTGC TGAAACCTGT GCAGAGGATA 
ACCAAGTACC AGCTGCTGCT CAAGGAAATG CTGAAGTACA GCAAGCACTG TGAAGGGGCA 
GAGGACCTGC AGGAGGCGCT GAGCTCCATC CTGGGCATCC TCAAGGCTGT GAACGACTCC 
ATGCATCTCA TCGCCATCAC TGGCTATGAT GGAAACCTTG GCGACCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGCGT GTGGACAGAC CACAAGAAGG GCCACACCAA AGTGAAGGAG 
CTAGCCAGGT TCAAGCCCAT GCAGCGGCAC CTGTTTCTGC ACGAGAAGGC CGTCCTCTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGA TACGAGAAGG CTCCTTCCTA CAGCTACAAG 
CAGTCCTTGA ATATGACGGC TGTCGGCATC ACAGAGAACG TGAAGGGTGA CACCAAGAAG 
TTCGAGATCT GGTACAACGC AAGGGAGGAG GTTTATATCA TTCAGGCGCC AACCCCCGAG 
ATTAAAGCAG CGTGGGTGAA TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCTTGC 
CGGGAGGCCA GCCAGCACCG AGCCCTGGAG CAGTCCCACA GCCTTCCTTT GCCCACGCCA 
TCCAGCACCA GTCCCACAAA AGGGAACACA AGAAATGTCA AGAAGCTCGA AGACCGGAAG 
ACGGACCCGC TCAGCCTGGA AGGCTACGTG AGCTCATCGT TACCAAAGCC CCCTGAGAAG 
GGCAAAGGCT GGAGCAAAAC ATCCCACTCC CTGGAGGCCC CTGAGGAGGA CGGAGGCTGG 
TCCAGTGCTG AGGAACTCAT CAACTCATCT GATGCTGAGG AAGACGGTGG AGTGGGCCCT 
AAGAAGCTGG TGCCGGGTAA ATACACAGTG GTGATGGATG ATGAGAAAGG GGGCCCTGAC 
ACCCTTGCCA TGAGGAGTGG AGATATGGTG GAGGTGGTGG AAGAAGGCGC TGAGGGCCTC 
TGGTATGTTC GGGACCTGAC CAGCAGCAAG GAGGGATGGG TGCCAGCCAG CAGCCTATCC 
ACCCTCCTGG GCAAATCCAG TTCAGCCCAG TGTCTGAGCA GCTCAGGTAA GATCCACTGT 
GCGCGCCAGC TGTGCCCTGA GCCTGCCGAG ATCCTCAGCC CAGAGCCCGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001152957.1
CDS	242..3535
Translation	MRPTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGKIHCARQLCPEPAEILSPEPV

Target ORF information:

RefSeq Version	NM_001159485.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159485.2

ORF Insert Sequence:

ATGAGACCCA CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC CGCTGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGACA GGACGGAAGC 
CCAGTCATCA CCTTCCCTGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATC 
TTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCTGTGA AGGCATCCGT CCTGCGGATA 
GCCGCATCTT TCCCAGCCAA CCTGCAGCTT GTCCTTGTTC TTCGGCCCAC GGGCTTTTTC 
CAGAGGACTC TCTCGGACAT TGCCTTCAAG TTCAACAGAG ATGAGTTTAA GATGAAAGTG 
CCGGTCATGA TGCTAAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCGCAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCACTCCA GGTGGCTGTG CCACCGCACA 
GCAATTGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCCC AGATGCTGCA GGCCTTTGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCGAAT GACGTCCAGT CAACCAGCCT GGTGCTCAGC 
GCACACACAG AGAAGAAGGC CAAAGTGAAG GAAGATCTGC AGTTAGCACT GAAGGAGGGG 
AACAGCATCC TGGAGAGCCT CAGAGAGCCA CTGGCTGAGA GCGCGGCCCA CAGTGTGAAC 
CAGGACCAGC TGGACAATCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAGACG 
GAGGCTGCCT TTGACGAATT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAAGG TTTCCGGGAG GTCAAAACCA CCTTGGATTC CATGTCCCAG 
AAGATAGCTG CTTTCACGGA TGTTGGCAAC AGCCTGGCCC ACGTGCAGCA CCTTCTGAAG 
GACTTGACCG CCTTTGAGGA GAAGTCCAGT GTGGCTGTGG ACAAGGCCCG CGCCCTGTCA 
CTGGAAGGTC AGCAGCTGAT TGAGAACAGG CACTACGCTG TGGACTCCAT CCACCCCAAG 
TGTGAAGAGC TCCAGCACCT CTGTGACCAC TTCGCCTCTG AGGTCACCAG GAGGCGGGGC 
CTGCTCAGCA AGTCCTTGGA GCTGCATAGC CTCCTGGAGA CGTCCATGAA GTGGAGCGAC 
GAGGGGATCT TCCTGCTGGC CTCGCAGCCT GTGGACAAAT GCCAGTCCCA GGATGGCGCA 
GAGGCGGCCC TCCAGGAGAT TGAGAAGTTT CTGGAGACCG GTGCAGAAAA TAAGATTCAG 
GAGCTCAATG AGATTTACAA GGAATACGAA TGCATCCTCA ACCAGGACCT TCTGGAACAC 
GTGCAAAAAG TCTTTCAGAA GCAAGAAAGC ACGGAGGAGA TGTTCCACCG CAGGCAAGCC 
AGCCTGAAGA AACTGGCAGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCCCGACCA 
GAGGCGCTTA CCAAGTCGCC CTCCCCCTCG CCAGGCTCCT GGCGAAGCTC TGAGAACTCC 
AGCTCTGAGG GCAACGCACT CCGCAGAGGG CCCTACAGGA GAGCCAAGAG CGAAATGAGT 
GAACCCCGGC AGGGCCGCAC CAGCTCCACA GGGGAAGAGG AAGAGAGCCT GGCCATCCTG 
CGCAGACACG TGATGAATGA GCTTCTGGAC ACCGAGCGGG CCTACGTGGA GGAGCTGCTC 
TGTGTCTTAG AGGGCTATGC CGCAGAGATG GATAACCCCT TGATGGCACA CCTCATCTCA 
ACAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAAAT CTATCATTTT 
CACAACAGAA TATTCCTGCG GGAGCTGGAG AGCTGCATAG ACTGCCCAGA GCTGGTGGGG 
AGGTGCTTTC TGGAAAGGAT GGAGGAGTTC CAGATCTATG AGAAGTACTG TCAGAACAAG 
CCACGCTCTG AGAGCCTGTG GAGGCAGTGC TCTGACTGCC CCTTCTTCCA GGAGTGTCAG 
AAGAAGCTGG ACCACAAGCT GAGCCTGGAC TCCTACCTGC TGAAACCTGT GCAGAGGATA 
ACCAAGTACC AGCTGCTGCT CAAGGAAATG CTGAAGTACA GCAAGCACTG TGAAGGGGCA 
GAGGACCTGC AGGAGGCGCT GAGCTCCATC CTGGGCATCC TCAAGGCTGT GAACGACTCC 
ATGCATCTCA TCGCCATCAC TGGCTATGAT GGAAACCTTG GCGACCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGCGT GTGGACAGAC CACAAGAAGG GCCACACCAA AGTGAAGGAG 
CTAGCCAGGT TCAAGCCCAT GCAGCGGCAC CTGTTTCTGC ACGAGAAGGC CGTCCTCTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGA TACGAGAAGG CTCCTTCCTA CAGCTACAAG 
CAGTCCTTGA ATATGACGGC TGTCGGCATC ACAGAGAACG TGAAGGGTGA CACCAAGAAG 
TTCGAGATCT GGTACAACGC AAGGGAGGAG GTTTATATCA TTCAGGCGCC AACCCCCGAG 
ATTAAAGCAG CGTGGGTGAA TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCTTGC 
CGGGAGGCCA GCCAGCACCG AGCCCTGGAG CAGTCCCACA GCCTTCCTTT GCCCACGCCA 
TCCAGCACCA GTCCCACAAA AGGGAACACA AGAAATGTCA AGAAGCTCGA AGACCGGAAG 
ACGGACCCGC TCAGCCTGGA AGGCTACGTG AGCTCATCGT TACCAAAGCC CCCTGAGAAG 
GGCAAAGGCT GGAGCAAAAC ATCCCACTCC CTGGAGGCCC CTGAGGAGGA CGGAGGCTGG 
TCCAGTGCTG AGGAACTCAT CAACTCATCT GATGCTGAGG AAGACGGTGG AGTGGGCCCT 
AAGAAGCTGG TGCCGGGTAA ATACACAGTG GTGATGGATG ATGAGAAAGG GGGCCCTGAC 
ACCCTTGCCA TGAGGAGTGG AGATATGGTG GAGGTGGTGG AAGAAGGCGC TGAGGGCCTC 
TGGTATGTTC GGGACCTGAC CAGCAGCAAG GAGGGATGGG TGCCAGCCAG CAGCCTATCC 
ACCCTCCTGG GCAAATCCAG TTCAGCCCAG TGTCTGAGCA GCTCAGGTAA GATCCACTGT 
GCGCGCCAGC TGTGCCCTGA GCCTGCCGAG ATCCTCAGCC CAGAGCCCGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( NM_001159486.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001152958.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 2, mRNA.

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CloneID	OMu07470
Clone ID Related Accession (Same CDS sequence)	NM_001159486.2 , NM_001159486.1
Accession Version	NM_001159486.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-24
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127308.3. On Jun 25, 2019 this sequence version replaced NM_001159486.1. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and lacks two alternate in-frame exons in the 3' coding region, compared to variant 1, resulting in an isoform (2) that has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK170186.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164136 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAGACCCA CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC CGCTGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGACA GGACGGAAGC 
CCAGTCATCA CCTTCCCTGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATC 
TTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCTGTGA AGGCATCCGT CCTGCGGATA 
GCCGCATCTT TCCCAGCCAA CCTGCAGCTT GTCCTTGTTC TTCGGCCCAC GGGCTTTTTC 
CAGAGGACTC TCTCGGACAT TGCCTTCAAG TTCAACAGAG ATGAGTTTAA GATGAAAGTG 
CCGGTCATGA TGCTAAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCGCAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCACTCCA GGTGGCTGTG CCACCGCACA 
GCAATTGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCCC AGATGCTGCA GGCCTTTGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCGAAT GACGTCCAGT CAACCAGCCT GGTGCTCAGC 
GCACACACAG AGAAGAAGGC CAAAGTGAAG GAAGATCTGC AGTTAGCACT GAAGGAGGGG 
AACAGCATCC TGGAGAGCCT CAGAGAGCCA CTGGCTGAGA GCGCGGCCCA CAGTGTGAAC 
CAGGACCAGC TGGACAATCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAGACG 
GAGGCTGCCT TTGACGAATT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAAGG TTTCCGGGAG GTCAAAACCA CCTTGGATTC CATGTCCCAG 
AAGATAGCTG CTTTCACGGA TGTTGGCAAC AGCCTGGCCC ACGTGCAGCA CCTTCTGAAG 
GACTTGACCG CCTTTGAGGA GAAGTCCAGT GTGGCTGTGG ACAAGGCCCG CGCCCTGTCA 
CTGGAAGGTC AGCAGCTGAT TGAGAACAGG CACTACGCTG TGGACTCCAT CCACCCCAAG 
TGTGAAGAGC TCCAGCACCT CTGTGACCAC TTCGCCTCTG AGGTCACCAG GAGGCGGGGC 
CTGCTCAGCA AGTCCTTGGA GCTGCATAGC CTCCTGGAGA CGTCCATGAA GTGGAGCGAC 
GAGGGGATCT TCCTGCTGGC CTCGCAGCCT GTGGACAAAT GCCAGTCCCA GGATGGCGCA 
GAGGCGGCCC TCCAGGAGAT TGAGAAGTTT CTGGAGACCG GTGCAGAAAA TAAGATTCAG 
GAGCTCAATG AGATTTACAA GGAATACGAA TGCATCCTCA ACCAGGACCT TCTGGAACAC 
GTGCAAAAAG TCTTTCAGAA GCAAGAAAGC ACGGAGGAGA TGTTCCACCG CAGGCAAGCC 
AGCCTGAAGA AACTGGCAGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCCCGACCA 
GAGGCGCTTA CCAAGTCGCC CTCCCCCTCG CCAGGCTCCT GGCGAAGCTC TGAGAACTCC 
AGCTCTGAGG GCAACGCACT CCGCAGAGGG CCCTACAGGA GAGCCAAGAG CGAAATGAGT 
GAACCCCGGC AGGGCCGCAC CAGCTCCACA GGGGAAGAGG AAGAGAGCCT GGCCATCCTG 
CGCAGACACG TGATGAATGA GCTTCTGGAC ACCGAGCGGG CCTACGTGGA GGAGCTGCTC 
TGTGTCTTAG AGGGCTATGC CGCAGAGATG GATAACCCCT TGATGGCACA CCTCATCTCA 
ACAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAAAT CTATCATTTT 
CACAACAGAA TATTCCTGCG GGAGCTGGAG AGCTGCATAG ACTGCCCAGA GCTGGTGGGG 
AGGTGCTTTC TGGAAAGGAT GGAGGAGTTC CAGATCTATG AGAAGTACTG TCAGAACAAG 
CCACGCTCTG AGAGCCTGTG GAGGCAGTGC TCTGACTGCC CCTTCTTCCA GGAGTGTCAG 
AAGAAGCTGG ACCACAAGCT GAGCCTGGAC TCCTACCTGC TGAAACCTGT GCAGAGGATA 
ACCAAGTACC AGCTGCTGCT CAAGGAAATG CTGAAGTACA GCAAGCACTG TGAAGGGGCA 
GAGGACCTGC AGGAGGCGCT GAGCTCCATC CTGGGCATCC TCAAGGCTGT GAACGACTCC 
ATGCATCTCA TCGCCATCAC TGGCTATGAT GGAAACCTTG GCGACCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGCGT GTGGACAGAC CACAAGAAGG GCCACACCAA AGTGAAGGAG 
CTAGCCAGGT TCAAGCCCAT GCAGCGGCAC CTGTTTCTGC ACGAGAAGGC CGTCCTCTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGA TACGAGAAGG CTCCTTCCTA CAGCTACAAG 
CAGTCCTTGA ATATGACGGC TGTCGGCATC ACAGAGAACG TGAAGGGTGA CACCAAGAAG 
TTCGAGATCT GGTACAACGC AAGGGAGGAG GTTTATATCA TTCAGGCGCC AACCCCCGAG 
ATTAAAGCAG CGTGGGTGAA TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCTTGC 
CGGGAGGCCA GCCAGCACCG AGCCCTGGAG CAGTCCCACA GCCTTCCTTT GCCCACGCCA 
TCCAGCACCA GTCCCACAAA AGGGAACACA AGAAATGTCA AGAAGCTCGA AGACCGGAAG 
ACGGACCCGC TCAGCCTGGA AGGCTACGTG AGCTCATCGT TACCAAAGCC CCCTGAGAAG 
GGCAAAGGCT GGAGCAAAAC ATCCCACTCC CTGGAGGCCC CTGAGGAGGA CGGAGGCTGG 
TCCAGTGCTG AGGAACTCAT CAACTCATCT GATGCTGAGG AAGACGGTGG AGTGGGCCCT 
AAGAAGCTGG TGCCGGGTAA ATACACAGTG GTGATGGATG ATGAGAAAGG GGGCCCTGAC 
ACCCTTGCCA TGAGGAGTGG AGATATGGTG GAGGTGGTGG AAGAAGGCGC TGAGGGCCTC 
TGGTATGTTC GGGACCTGAC CAGCAGCAAG GAGGGATGGG TGCCAGCCAG CAGCCTATCC 
ACCCTCCTGG GCAAATCCAG TTCAGCCCAG TGTCTGAGCA GCTCAGGTGT GCAGAGCAAA 
GGGCCAGAGC CGAAGCCTGT CTCCACTGGT TTCCCGCAGA CTCCAGCCCA GGGTCAGCCG 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001152958.1
CDS	242..3547
Translation	MRPTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGVQSKGPEPKPVSTGFPQTPAQGQPC

Target ORF information:

RefSeq Version	NM_001159486.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159486.2

ORF Insert Sequence:

ATGAGACCCA CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC CGCTGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGACA GGACGGAAGC 
CCAGTCATCA CCTTCCCTGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATC 
TTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCTGTGA AGGCATCCGT CCTGCGGATA 
GCCGCATCTT TCCCAGCCAA CCTGCAGCTT GTCCTTGTTC TTCGGCCCAC GGGCTTTTTC 
CAGAGGACTC TCTCGGACAT TGCCTTCAAG TTCAACAGAG ATGAGTTTAA GATGAAAGTG 
CCGGTCATGA TGCTAAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCGCAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCACTCCA GGTGGCTGTG CCACCGCACA 
GCAATTGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCCC AGATGCTGCA GGCCTTTGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCGAAT GACGTCCAGT CAACCAGCCT GGTGCTCAGC 
GCACACACAG AGAAGAAGGC CAAAGTGAAG GAAGATCTGC AGTTAGCACT GAAGGAGGGG 
AACAGCATCC TGGAGAGCCT CAGAGAGCCA CTGGCTGAGA GCGCGGCCCA CAGTGTGAAC 
CAGGACCAGC TGGACAATCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAGACG 
GAGGCTGCCT TTGACGAATT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAAGG TTTCCGGGAG GTCAAAACCA CCTTGGATTC CATGTCCCAG 
AAGATAGCTG CTTTCACGGA TGTTGGCAAC AGCCTGGCCC ACGTGCAGCA CCTTCTGAAG 
GACTTGACCG CCTTTGAGGA GAAGTCCAGT GTGGCTGTGG ACAAGGCCCG CGCCCTGTCA 
CTGGAAGGTC AGCAGCTGAT TGAGAACAGG CACTACGCTG TGGACTCCAT CCACCCCAAG 
TGTGAAGAGC TCCAGCACCT CTGTGACCAC TTCGCCTCTG AGGTCACCAG GAGGCGGGGC 
CTGCTCAGCA AGTCCTTGGA GCTGCATAGC CTCCTGGAGA CGTCCATGAA GTGGAGCGAC 
GAGGGGATCT TCCTGCTGGC CTCGCAGCCT GTGGACAAAT GCCAGTCCCA GGATGGCGCA 
GAGGCGGCCC TCCAGGAGAT TGAGAAGTTT CTGGAGACCG GTGCAGAAAA TAAGATTCAG 
GAGCTCAATG AGATTTACAA GGAATACGAA TGCATCCTCA ACCAGGACCT TCTGGAACAC 
GTGCAAAAAG TCTTTCAGAA GCAAGAAAGC ACGGAGGAGA TGTTCCACCG CAGGCAAGCC 
AGCCTGAAGA AACTGGCAGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCCCGACCA 
GAGGCGCTTA CCAAGTCGCC CTCCCCCTCG CCAGGCTCCT GGCGAAGCTC TGAGAACTCC 
AGCTCTGAGG GCAACGCACT CCGCAGAGGG CCCTACAGGA GAGCCAAGAG CGAAATGAGT 
GAACCCCGGC AGGGCCGCAC CAGCTCCACA GGGGAAGAGG AAGAGAGCCT GGCCATCCTG 
CGCAGACACG TGATGAATGA GCTTCTGGAC ACCGAGCGGG CCTACGTGGA GGAGCTGCTC 
TGTGTCTTAG AGGGCTATGC CGCAGAGATG GATAACCCCT TGATGGCACA CCTCATCTCA 
ACAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAAAT CTATCATTTT 
CACAACAGAA TATTCCTGCG GGAGCTGGAG AGCTGCATAG ACTGCCCAGA GCTGGTGGGG 
AGGTGCTTTC TGGAAAGGAT GGAGGAGTTC CAGATCTATG AGAAGTACTG TCAGAACAAG 
CCACGCTCTG AGAGCCTGTG GAGGCAGTGC TCTGACTGCC CCTTCTTCCA GGAGTGTCAG 
AAGAAGCTGG ACCACAAGCT GAGCCTGGAC TCCTACCTGC TGAAACCTGT GCAGAGGATA 
ACCAAGTACC AGCTGCTGCT CAAGGAAATG CTGAAGTACA GCAAGCACTG TGAAGGGGCA 
GAGGACCTGC AGGAGGCGCT GAGCTCCATC CTGGGCATCC TCAAGGCTGT GAACGACTCC 
ATGCATCTCA TCGCCATCAC TGGCTATGAT GGAAACCTTG GCGACCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGCGT GTGGACAGAC CACAAGAAGG GCCACACCAA AGTGAAGGAG 
CTAGCCAGGT TCAAGCCCAT GCAGCGGCAC CTGTTTCTGC ACGAGAAGGC CGTCCTCTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGA TACGAGAAGG CTCCTTCCTA CAGCTACAAG 
CAGTCCTTGA ATATGACGGC TGTCGGCATC ACAGAGAACG TGAAGGGTGA CACCAAGAAG 
TTCGAGATCT GGTACAACGC AAGGGAGGAG GTTTATATCA TTCAGGCGCC AACCCCCGAG 
ATTAAAGCAG CGTGGGTGAA TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCTTGC 
CGGGAGGCCA GCCAGCACCG AGCCCTGGAG CAGTCCCACA GCCTTCCTTT GCCCACGCCA 
TCCAGCACCA GTCCCACAAA AGGGAACACA AGAAATGTCA AGAAGCTCGA AGACCGGAAG 
ACGGACCCGC TCAGCCTGGA AGGCTACGTG AGCTCATCGT TACCAAAGCC CCCTGAGAAG 
GGCAAAGGCT GGAGCAAAAC ATCCCACTCC CTGGAGGCCC CTGAGGAGGA CGGAGGCTGG 
TCCAGTGCTG AGGAACTCAT CAACTCATCT GATGCTGAGG AAGACGGTGG AGTGGGCCCT 
AAGAAGCTGG TGCCGGGTAA ATACACAGTG GTGATGGATG ATGAGAAAGG GGGCCCTGAC 
ACCCTTGCCA TGAGGAGTGG AGATATGGTG GAGGTGGTGG AAGAAGGCGC TGAGGGCCTC 
TGGTATGTTC GGGACCTGAC CAGCAGCAAG GAGGGATGGG TGCCAGCCAG CAGCCTATCC 
ACCCTCCTGG GCAAATCCAG TTCAGCCCAG TGTCTGAGCA GCTCAGGTGT GCAGAGCAAA 
GGGCCAGAGC CGAAGCCTGT CTCCACTGGT TTCCCGCAGA CTCCAGCCCA GGGTCAGCCG 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( NM_001159486.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001152958.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 2, mRNA.

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CloneID	OMu07470
Clone ID Related Accession (Same CDS sequence)	NM_001159486.2 , NM_001159486.1
Accession Version	NM_001159486.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY202611.1, BC058622.1, BQ555281.1 and AI853669.1. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and lacks two alternate in-frame exons in the 3' coding region, compared to variant 1, resulting in an isoform (2) that has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK170186.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164136 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGACCCA CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC CGCTGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGACA GGACGGAAGC 
CCAGTCATCA CCTTCCCTGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATC 
TTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCTGTGA AGGCATCCGT CCTGCGGATA 
GCCGCATCTT TCCCAGCCAA CCTGCAGCTT GTCCTTGTTC TTCGGCCCAC GGGCTTTTTC 
CAGAGGACTC TCTCGGACAT TGCCTTCAAG TTCAACAGAG ATGAGTTTAA GATGAAAGTG 
CCGGTCATGA TGCTAAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCGCAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCACTCCA GGTGGCTGTG CCACCGCACA 
GCAATTGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCCC AGATGCTGCA GGCCTTTGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCGAAT GACGTCCAGT CAACCAGCCT GGTGCTCAGC 
GCACACACAG AGAAGAAGGC CAAAGTGAAG GAAGATCTGC AGTTAGCACT GAAGGAGGGG 
AACAGCATCC TGGAGAGCCT CAGAGAGCCA CTGGCTGAGA GCGCGGCCCA CAGTGTGAAC 
CAGGACCAGC TGGACAATCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAGACG 
GAGGCTGCCT TTGACGAATT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAAGG TTTCCGGGAG GTCAAAACCA CCTTGGATTC CATGTCCCAG 
AAGATAGCTG CTTTCACGGA TGTTGGCAAC AGCCTGGCCC ACGTGCAGCA CCTTCTGAAG 
GACTTGACCG CCTTTGAGGA GAAGTCCAGT GTGGCTGTGG ACAAGGCCCG CGCCCTGTCA 
CTGGAAGGTC AGCAGCTGAT TGAGAACAGG CACTACGCTG TGGACTCCAT CCACCCCAAG 
TGTGAAGAGC TCCAGCACCT CTGTGACCAC TTCGCCTCTG AGGTCACCAG GAGGCGGGGC 
CTGCTCAGCA AGTCCTTGGA GCTGCATAGC CTCCTGGAGA CGTCCATGAA GTGGAGCGAC 
GAGGGGATCT TCCTGCTGGC CTCGCAGCCT GTGGACAAAT GCCAGTCCCA GGATGGCGCA 
GAGGCGGCCC TCCAGGAGAT TGAGAAGTTT CTGGAGACCG GTGCAGAAAA TAAGATTCAG 
GAGCTCAATG AGATTTACAA GGAATACGAA TGCATCCTCA ACCAGGACCT TCTGGAACAC 
GTGCAAAAAG TCTTTCAGAA GCAAGAAAGC ACGGAGGAGA TGTTCCACCG CAGGCAAGCC 
AGCCTGAAGA AACTGGCAGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCCCGACCA 
GAGGCGCTTA CCAAGTCGCC CTCCCCCTCG CCAGGCTCCT GGCGAAGCTC TGAGAACTCC 
AGCTCTGAGG GCAACGCACT CCGCAGAGGG CCCTACAGGA GAGCCAAGAG CGAAATGAGT 
GAACCCCGGC AGGGCCGCAC CAGCTCCACA GGGGAAGAGG AAGAGAGCCT GGCCATCCTG 
CGCAGACACG TGATGAATGA GCTTCTGGAC ACCGAGCGGG CCTACGTGGA GGAGCTGCTC 
TGTGTCTTAG AGGGCTATGC CGCAGAGATG GATAACCCCT TGATGGCACA CCTCATCTCA 
ACAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAAAT CTATCATTTT 
CACAACAGAA TATTCCTGCG GGAGCTGGAG AGCTGCATAG ACTGCCCAGA GCTGGTGGGG 
AGGTGCTTTC TGGAAAGGAT GGAGGAGTTC CAGATCTATG AGAAGTACTG TCAGAACAAG 
CCACGCTCTG AGAGCCTGTG GAGGCAGTGC TCTGACTGCC CCTTCTTCCA GGAGTGTCAG 
AAGAAGCTGG ACCACAAGCT GAGCCTGGAC TCCTACCTGC TGAAACCTGT GCAGAGGATA 
ACCAAGTACC AGCTGCTGCT CAAGGAAATG CTGAAGTACA GCAAGCACTG TGAAGGGGCA 
GAGGACCTGC AGGAGGCGCT GAGCTCCATC CTGGGCATCC TCAAGGCTGT GAACGACTCC 
ATGCATCTCA TCGCCATCAC TGGCTATGAT GGAAACCTTG GCGACCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGCGT GTGGACAGAC CACAAGAAGG GCCACACCAA AGTGAAGGAG 
CTAGCCAGGT TCAAGCCCAT GCAGCGGCAC CTGTTTCTGC ACGAGAAGGC CGTCCTCTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGA TACGAGAAGG CTCCTTCCTA CAGCTACAAG 
CAGTCCTTGA ATATGACGGC TGTCGGCATC ACAGAGAACG TGAAGGGTGA CACCAAGAAG 
TTCGAGATCT GGTACAACGC AAGGGAGGAG GTTTATATCA TTCAGGCGCC AACCCCCGAG 
ATTAAAGCAG CGTGGGTGAA TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCTTGC 
CGGGAGGCCA GCCAGCACCG AGCCCTGGAG CAGTCCCACA GCCTTCCTTT GCCCACGCCA 
TCCAGCACCA GTCCCACAAA AGGGAACACA AGAAATGTCA AGAAGCTCGA AGACCGGAAG 
ACGGACCCGC TCAGCCTGGA AGGCTACGTG AGCTCATCGT TACCAAAGCC CCCTGAGAAG 
GGCAAAGGCT GGAGCAAAAC ATCCCACTCC CTGGAGGCCC CTGAGGAGGA CGGAGGCTGG 
TCCAGTGCTG AGGAACTCAT CAACTCATCT GATGCTGAGG AAGACGGTGG AGTGGGCCCT 
AAGAAGCTGG TGCCGGGTAA ATACACAGTG GTGATGGATG ATGAGAAAGG GGGCCCTGAC 
ACCCTTGCCA TGAGGAGTGG AGATATGGTG GAGGTGGTGG AAGAAGGCGC TGAGGGCCTC 
TGGTATGTTC GGGACCTGAC CAGCAGCAAG GAGGGATGGG TGCCAGCCAG CAGCCTATCC 
ACCCTCCTGG GCAAATCCAG TTCAGCCCAG TGTCTGAGCA GCTCAGGTGT GCAGAGCAAA 
GGGCCAGAGC CGAAGCCTGT CTCCACTGGT TTCCCGCAGA CTCCAGCCCA GGGTCAGCCG 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001152958.1
CDS	247..3552
Translation	MRPTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGVQSKGPEPKPVSTGFPQTPAQGQPC

Target ORF information:

RefSeq Version	NM_001159486.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159486.1

ORF Insert Sequence:

ATGAGACCCA CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC CGCTGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGACA GGACGGAAGC 
CCAGTCATCA CCTTCCCTGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATC 
TTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCTGTGA AGGCATCCGT CCTGCGGATA 
GCCGCATCTT TCCCAGCCAA CCTGCAGCTT GTCCTTGTTC TTCGGCCCAC GGGCTTTTTC 
CAGAGGACTC TCTCGGACAT TGCCTTCAAG TTCAACAGAG ATGAGTTTAA GATGAAAGTG 
CCGGTCATGA TGCTAAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCGCAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCACTCCA GGTGGCTGTG CCACCGCACA 
GCAATTGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCCC AGATGCTGCA GGCCTTTGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCGAAT GACGTCCAGT CAACCAGCCT GGTGCTCAGC 
GCACACACAG AGAAGAAGGC CAAAGTGAAG GAAGATCTGC AGTTAGCACT GAAGGAGGGG 
AACAGCATCC TGGAGAGCCT CAGAGAGCCA CTGGCTGAGA GCGCGGCCCA CAGTGTGAAC 
CAGGACCAGC TGGACAATCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAGACG 
GAGGCTGCCT TTGACGAATT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAAGG TTTCCGGGAG GTCAAAACCA CCTTGGATTC CATGTCCCAG 
AAGATAGCTG CTTTCACGGA TGTTGGCAAC AGCCTGGCCC ACGTGCAGCA CCTTCTGAAG 
GACTTGACCG CCTTTGAGGA GAAGTCCAGT GTGGCTGTGG ACAAGGCCCG CGCCCTGTCA 
CTGGAAGGTC AGCAGCTGAT TGAGAACAGG CACTACGCTG TGGACTCCAT CCACCCCAAG 
TGTGAAGAGC TCCAGCACCT CTGTGACCAC TTCGCCTCTG AGGTCACCAG GAGGCGGGGC 
CTGCTCAGCA AGTCCTTGGA GCTGCATAGC CTCCTGGAGA CGTCCATGAA GTGGAGCGAC 
GAGGGGATCT TCCTGCTGGC CTCGCAGCCT GTGGACAAAT GCCAGTCCCA GGATGGCGCA 
GAGGCGGCCC TCCAGGAGAT TGAGAAGTTT CTGGAGACCG GTGCAGAAAA TAAGATTCAG 
GAGCTCAATG AGATTTACAA GGAATACGAA TGCATCCTCA ACCAGGACCT TCTGGAACAC 
GTGCAAAAAG TCTTTCAGAA GCAAGAAAGC ACGGAGGAGA TGTTCCACCG CAGGCAAGCC 
AGCCTGAAGA AACTGGCAGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCCCGACCA 
GAGGCGCTTA CCAAGTCGCC CTCCCCCTCG CCAGGCTCCT GGCGAAGCTC TGAGAACTCC 
AGCTCTGAGG GCAACGCACT CCGCAGAGGG CCCTACAGGA GAGCCAAGAG CGAAATGAGT 
GAACCCCGGC AGGGCCGCAC CAGCTCCACA GGGGAAGAGG AAGAGAGCCT GGCCATCCTG 
CGCAGACACG TGATGAATGA GCTTCTGGAC ACCGAGCGGG CCTACGTGGA GGAGCTGCTC 
TGTGTCTTAG AGGGCTATGC CGCAGAGATG GATAACCCCT TGATGGCACA CCTCATCTCA 
ACAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAAAT CTATCATTTT 
CACAACAGAA TATTCCTGCG GGAGCTGGAG AGCTGCATAG ACTGCCCAGA GCTGGTGGGG 
AGGTGCTTTC TGGAAAGGAT GGAGGAGTTC CAGATCTATG AGAAGTACTG TCAGAACAAG 
CCACGCTCTG AGAGCCTGTG GAGGCAGTGC TCTGACTGCC CCTTCTTCCA GGAGTGTCAG 
AAGAAGCTGG ACCACAAGCT GAGCCTGGAC TCCTACCTGC TGAAACCTGT GCAGAGGATA 
ACCAAGTACC AGCTGCTGCT CAAGGAAATG CTGAAGTACA GCAAGCACTG TGAAGGGGCA 
GAGGACCTGC AGGAGGCGCT GAGCTCCATC CTGGGCATCC TCAAGGCTGT GAACGACTCC 
ATGCATCTCA TCGCCATCAC TGGCTATGAT GGAAACCTTG GCGACCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGCGT GTGGACAGAC CACAAGAAGG GCCACACCAA AGTGAAGGAG 
CTAGCCAGGT TCAAGCCCAT GCAGCGGCAC CTGTTTCTGC ACGAGAAGGC CGTCCTCTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGA TACGAGAAGG CTCCTTCCTA CAGCTACAAG 
CAGTCCTTGA ATATGACGGC TGTCGGCATC ACAGAGAACG TGAAGGGTGA CACCAAGAAG 
TTCGAGATCT GGTACAACGC AAGGGAGGAG GTTTATATCA TTCAGGCGCC AACCCCCGAG 
ATTAAAGCAG CGTGGGTGAA TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCTTGC 
CGGGAGGCCA GCCAGCACCG AGCCCTGGAG CAGTCCCACA GCCTTCCTTT GCCCACGCCA 
TCCAGCACCA GTCCCACAAA AGGGAACACA AGAAATGTCA AGAAGCTCGA AGACCGGAAG 
ACGGACCCGC TCAGCCTGGA AGGCTACGTG AGCTCATCGT TACCAAAGCC CCCTGAGAAG 
GGCAAAGGCT GGAGCAAAAC ATCCCACTCC CTGGAGGCCC CTGAGGAGGA CGGAGGCTGG 
TCCAGTGCTG AGGAACTCAT CAACTCATCT GATGCTGAGG AAGACGGTGG AGTGGGCCCT 
AAGAAGCTGG TGCCGGGTAA ATACACAGTG GTGATGGATG ATGAGAAAGG GGGCCCTGAC 
ACCCTTGCCA TGAGGAGTGG AGATATGGTG GAGGTGGTGG AAGAAGGCGC TGAGGGCCTC 
TGGTATGTTC GGGACCTGAC CAGCAGCAAG GAGGGATGGG TGCCAGCCAG CAGCCTATCC 
ACCCTCCTGG GCAAATCCAG TTCAGCCCAG TGTCTGAGCA GCTCAGGTGT GCAGAGCAAA 
GGGCCAGAGC CGAAGCCTGT CTCCACTGGT TTCCCGCAGA CTCCAGCCCA GGGTCAGCCG 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_017312593.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168082.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X3, mRNA.

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CloneID	OMu40334
Clone ID Related Accession (Same CDS sequence)	XM_017312593.1 , XM_006508706.3 , XM_006508707.2 , XM_011242040.1
Accession Version	XM_017312593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGACGATGC GGTCCCTAGT 
TCTACCTCTG AAAGCTCTGC GCTTTCCAGA AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC 
CTCAAAGGTC AGAAAGCCTC TCCGACCAGT CCTGACAAAA AAGCTAAGCG GCACGAAGTA 
AAGAGCGACC CGACTCCCTT TGGTTTGCGA GGCTGGAGCA AAACATCCCA CTCCCTGGAG 
GCCCCTGAGG AGGACGGAGG CTGGTCCAGT GCTGAGGAAC TCATCAACTC ATCTGATGCT 
GAGGAAGACG GTGGAGTGGG CCCTAAGAAG CTGGTGCCGG GTAAATACAC AGTGGTGATG 
GATGATGAGA AAGGGGGCCC TGACACCCTT GCCATGAGGA GTGGAGATAT GGTGGAGGTG 
GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT GTTCGGGACC TGACCAGCAG CAAGGAGGGA 
TGGGTGCCAG CCAGCAGCCT ATCCACCCTC CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG 
AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC GTGCTGAGCA GCTCATCCAG CTTCAGCGAG 
GGCTACCCTG CTCCCTTCTC CGAGCTCCAG GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168082.1
CDS	82..3717
Translation	MSDCWCFIFCKEHVRSNPLSPQHDGASREEADHQVDVSDGIRLVPDKAEATAATASDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	XM_017312593.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312593.1

ORF Insert Sequence:

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGACGATGC GGTCCCTAGT 
TCTACCTCTG AAAGCTCTGC GCTTTCCAGA AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC 
CTCAAAGGTC AGAAAGCCTC TCCGACCAGT CCTGACAAAA AAGCTAAGCG GCACGAAGTA 
AAGAGCGACC CGACTCCCTT TGGTTTGCGA GGCTGGAGCA AAACATCCCA CTCCCTGGAG 
GCCCCTGAGG AGGACGGAGG CTGGTCCAGT GCTGAGGAAC TCATCAACTC ATCTGATGCT 
GAGGAAGACG GTGGAGTGGG CCCTAAGAAG CTGGTGCCGG GTAAATACAC AGTGGTGATG 
GATGATGAGA AAGGGGGCCC TGACACCCTT GCCATGAGGA GTGGAGATAT GGTGGAGGTG 
GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT GTTCGGGACC TGACCAGCAG CAAGGAGGGA 
TGGGTGCCAG CCAGCAGCCT ATCCACCCTC CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG 
AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC GTGCTGAGCA GCTCATCCAG CTTCAGCGAG 
GGCTACCCTG CTCCCTTCTC CGAGCTCCAG GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508706.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508769.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X1, mRNA.

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CloneID	OMu40334
Clone ID Related Accession (Same CDS sequence)	XM_017312593.1 , XM_006508706.3 , XM_006508707.2 , XM_011242040.1
Accession Version	XM_006508706.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGACGATGC GGTCCCTAGT 
TCTACCTCTG AAAGCTCTGC GCTTTCCAGA AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC 
CTCAAAGGTC AGAAAGCCTC TCCGACCAGT CCTGACAAAA AAGCTAAGCG GCACGAAGTA 
AAGAGCGACC CGACTCCCTT TGGTTTGCGA GGCTGGAGCA AAACATCCCA CTCCCTGGAG 
GCCCCTGAGG AGGACGGAGG CTGGTCCAGT GCTGAGGAAC TCATCAACTC ATCTGATGCT 
GAGGAAGACG GTGGAGTGGG CCCTAAGAAG CTGGTGCCGG GTAAATACAC AGTGGTGATG 
GATGATGAGA AAGGGGGCCC TGACACCCTT GCCATGAGGA GTGGAGATAT GGTGGAGGTG 
GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT GTTCGGGACC TGACCAGCAG CAAGGAGGGA 
TGGGTGCCAG CCAGCAGCCT ATCCACCCTC CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG 
AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC GTGCTGAGCA GCTCATCCAG CTTCAGCGAG 
GGCTACCCTG CTCCCTTCTC CGAGCTCCAG GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508769.1
CDS	302..3937
Translation	MSDCWCFIFCKEHVRSNPLSPQHDGASREEADHQVDVSDGIRLVPDKAEATAATASDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	XM_006508706.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508706.3

ORF Insert Sequence:

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGACGATGC GGTCCCTAGT 
TCTACCTCTG AAAGCTCTGC GCTTTCCAGA AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC 
CTCAAAGGTC AGAAAGCCTC TCCGACCAGT CCTGACAAAA AAGCTAAGCG GCACGAAGTA 
AAGAGCGACC CGACTCCCTT TGGTTTGCGA GGCTGGAGCA AAACATCCCA CTCCCTGGAG 
GCCCCTGAGG AGGACGGAGG CTGGTCCAGT GCTGAGGAAC TCATCAACTC ATCTGATGCT 
GAGGAAGACG GTGGAGTGGG CCCTAAGAAG CTGGTGCCGG GTAAATACAC AGTGGTGATG 
GATGATGAGA AAGGGGGCCC TGACACCCTT GCCATGAGGA GTGGAGATAT GGTGGAGGTG 
GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT GTTCGGGACC TGACCAGCAG CAAGGAGGGA 
TGGGTGCCAG CCAGCAGCCT ATCCACCCTC CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG 
AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC GTGCTGAGCA GCTCATCCAG CTTCAGCGAG 
GGCTACCCTG CTCCCTTCTC CGAGCTCCAG GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508707.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508770.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu40334
Clone ID Related Accession (Same CDS sequence)	XM_017312593.1 , XM_006508706.3 , XM_006508707.2 , XM_011242040.1
Accession Version	XM_006508707.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006508707.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGACGATGC GGTCCCTAGT 
TCTACCTCTG AAAGCTCTGC GCTTTCCAGA AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC 
CTCAAAGGTC AGAAAGCCTC TCCGACCAGT CCTGACAAAA AAGCTAAGCG GCACGAAGTA 
AAGAGCGACC CGACTCCCTT TGGTTTGCGA GGCTGGAGCA AAACATCCCA CTCCCTGGAG 
GCCCCTGAGG AGGACGGAGG CTGGTCCAGT GCTGAGGAAC TCATCAACTC ATCTGATGCT 
GAGGAAGACG GTGGAGTGGG CCCTAAGAAG CTGGTGCCGG GTAAATACAC AGTGGTGATG 
GATGATGAGA AAGGGGGCCC TGACACCCTT GCCATGAGGA GTGGAGATAT GGTGGAGGTG 
GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT GTTCGGGACC TGACCAGCAG CAAGGAGGGA 
TGGGTGCCAG CCAGCAGCCT ATCCACCCTC CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG 
AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC GTGCTGAGCA GCTCATCCAG CTTCAGCGAG 
GGCTACCCTG CTCCCTTCTC CGAGCTCCAG GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508770.1
CDS	352..3987
Translation	MSDCWCFIFCKEHVRSNPLSPQHDGASREEADHQVDVSDGIRLVPDKAEATAATASDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	XM_006508707.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508707.2

ORF Insert Sequence:

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGACGATGC GGTCCCTAGT 
TCTACCTCTG AAAGCTCTGC GCTTTCCAGA AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC 
CTCAAAGGTC AGAAAGCCTC TCCGACCAGT CCTGACAAAA AAGCTAAGCG GCACGAAGTA 
AAGAGCGACC CGACTCCCTT TGGTTTGCGA GGCTGGAGCA AAACATCCCA CTCCCTGGAG 
GCCCCTGAGG AGGACGGAGG CTGGTCCAGT GCTGAGGAAC TCATCAACTC ATCTGATGCT 
GAGGAAGACG GTGGAGTGGG CCCTAAGAAG CTGGTGCCGG GTAAATACAC AGTGGTGATG 
GATGATGAGA AAGGGGGCCC TGACACCCTT GCCATGAGGA GTGGAGATAT GGTGGAGGTG 
GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT GTTCGGGACC TGACCAGCAG CAAGGAGGGA 
TGGGTGCCAG CCAGCAGCCT ATCCACCCTC CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG 
AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC GTGCTGAGCA GCTCATCCAG CTTCAGCGAG 
GGCTACCCTG CTCCCTTCTC CGAGCTCCAG GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_011242040.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240342.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OMu40334
Clone ID Related Accession (Same CDS sequence)	XM_017312593.1 , XM_006508706.3 , XM_006508707.2 , XM_011242040.1
Accession Version	XM_011242040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGACGATGC GGTCCCTAGT 
TCTACCTCTG AAAGCTCTGC GCTTTCCAGA AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC 
CTCAAAGGTC AGAAAGCCTC TCCGACCAGT CCTGACAAAA AAGCTAAGCG GCACGAAGTA 
AAGAGCGACC CGACTCCCTT TGGTTTGCGA GGCTGGAGCA AAACATCCCA CTCCCTGGAG 
GCCCCTGAGG AGGACGGAGG CTGGTCCAGT GCTGAGGAAC TCATCAACTC ATCTGATGCT 
GAGGAAGACG GTGGAGTGGG CCCTAAGAAG CTGGTGCCGG GTAAATACAC AGTGGTGATG 
GATGATGAGA AAGGGGGCCC TGACACCCTT GCCATGAGGA GTGGAGATAT GGTGGAGGTG 
GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT GTTCGGGACC TGACCAGCAG CAAGGAGGGA 
TGGGTGCCAG CCAGCAGCCT ATCCACCCTC CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG 
AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC GTGCTGAGCA GCTCATCCAG CTTCAGCGAG 
GGCTACCCTG CTCCCTTCTC CGAGCTCCAG GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240342.1
CDS	95..3730
Translation	MSDCWCFIFCKEHVRSNPLSPQHDGASREEADHQVDVSDGIRLVPDKAEATAATASDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	XM_011242040.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242040.1

ORF Insert Sequence:

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGACGATGC GGTCCCTAGT 
TCTACCTCTG AAAGCTCTGC GCTTTCCAGA AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC 
CTCAAAGGTC AGAAAGCCTC TCCGACCAGT CCTGACAAAA AAGCTAAGCG GCACGAAGTA 
AAGAGCGACC CGACTCCCTT TGGTTTGCGA GGCTGGAGCA AAACATCCCA CTCCCTGGAG 
GCCCCTGAGG AGGACGGAGG CTGGTCCAGT GCTGAGGAAC TCATCAACTC ATCTGATGCT 
GAGGAAGACG GTGGAGTGGG CCCTAAGAAG CTGGTGCCGG GTAAATACAC AGTGGTGATG 
GATGATGAGA AAGGGGGCCC TGACACCCTT GCCATGAGGA GTGGAGATAT GGTGGAGGTG 
GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT GTTCGGGACC TGACCAGCAG CAAGGAGGGA 
TGGGTGCCAG CCAGCAGCCT ATCCACCCTC CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG 
AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC GTGCTGAGCA GCTCATCCAG CTTCAGCGAG 
GGCTACCCTG CTCCCTTCTC CGAGCTCCAG GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508708.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508771.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X4, mRNA.

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CloneID	OMu40335
Clone ID Related Accession (Same CDS sequence)	XM_006508708.3
Accession Version	XM_006508708.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508708.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGACGATGC GGTCCCTAGT 
TCTACCTCTG AAAGCTCTGC GCTTTCCAGA AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC 
CTCAAAGGTC AGAAAGCCTC TCCGACCAGT CCTGACAAAA AAGCTAAGCG GCACGAAGTA 
AAGAGCGACC CGACTCCCTT TGGTTTGCGA GGCTGGAGCA AAACATCCCA CTCCCTGGAG 
GCCCCTGAGG AGGACGGAGG CTGGTCCAGT GCTGAGGAAC TCATCAACTC ATCTGATGCT 
GAGGAAGACG GTGGAGTGGG CCCTAAGAAG CTGGTGCCGG GTAAATACAC AGTGGTGATG 
GATGATGAGA AAGGGGGCCC TGACACCCTT GCCATGAGGA GTGGAGATAT GGTGGAGGTG 
GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT GTTCGGGACC TGACCAGCAG CAAGGAGGGA 
TGGGTGCCAG CCAGCAGCCT ATCCACCCTC CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG 
AGCAGCTCAG GTGTGCAGAG CAAAGGGCCA GAGCCGAAGC CTGTCTCCAC TGGTTTCCCG 
CAGACTCCAG CCCAGGGTCA GCCGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508771.1
CDS	302..3931
Translation	MSDCWCFIFCKEHVRSNPLSPQHDGASREEADHQVDVSDGIRLVPDKAEATAATASDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGVQSKGPEPKPVSTGFPQTPAQGQPC

Target ORF information:

RefSeq Version	XM_006508708.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508708.3

ORF Insert Sequence:

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGACGATGC GGTCCCTAGT 
TCTACCTCTG AAAGCTCTGC GCTTTCCAGA AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC 
CTCAAAGGTC AGAAAGCCTC TCCGACCAGT CCTGACAAAA AAGCTAAGCG GCACGAAGTA 
AAGAGCGACC CGACTCCCTT TGGTTTGCGA GGCTGGAGCA AAACATCCCA CTCCCTGGAG 
GCCCCTGAGG AGGACGGAGG CTGGTCCAGT GCTGAGGAAC TCATCAACTC ATCTGATGCT 
GAGGAAGACG GTGGAGTGGG CCCTAAGAAG CTGGTGCCGG GTAAATACAC AGTGGTGATG 
GATGATGAGA AAGGGGGCCC TGACACCCTT GCCATGAGGA GTGGAGATAT GGTGGAGGTG 
GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT GTTCGGGACC TGACCAGCAG CAAGGAGGGA 
TGGGTGCCAG CCAGCAGCCT ATCCACCCTC CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG 
AGCAGCTCAG GTGTGCAGAG CAAAGGGCCA GAGCCGAAGC CTGTCTCCAC TGGTTTCCCG 
CAGACTCCAG CCCAGGGTCA GCCGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508709.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508772.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu40336
Clone ID Related Accession (Same CDS sequence)	XM_006508709.3
Accession Version	XM_006508709.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508709.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGACGATGC GGTCCCTAGT 
TCTACCTCTG AAAGCTCTGC GCTTTCCAGA AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC 
CTCAAAGCCT CTCCGACCAG TCCTGACAAA AAAGCTAAGC GGCACGAAGT AAAGAGCGAC 
CCGACTCCCT TTGGTTTGCG AGGCTGGAGC AAAACATCCC ACTCCCTGGA GGCCCCTGAG 
GAGGACGGAG GCTGGTCCAG TGCTGAGGAA CTCATCAACT CATCTGATGC TGAGGAAGAC 
GGTGGAGTGG GCCCTAAGAA GCTGGTGCCG GGTAAATACA CAGTGGTGAT GGATGATGAG 
AAAGGGGGCC CTGACACCCT TGCCATGAGG AGTGGAGATA TGGTGGAGGT GGTGGAAGAA 
GGCGCTGAGG GCCTCTGGTA TGTTCGGGAC CTGACCAGCA GCAAGGAGGG ATGGGTGCCA 
GCCAGCAGCC TATCCACCCT CCTGGGCAAA TCCAGTTCAG CCCAGTGTCT GAGCAGCTCA 
GACTCCAGCC CAGGGTCAGC CGTGCTGAGC AGCTCATCCA GCTTCAGCGA GGGCTACCCT 
GCTCCCTTCT CCGAGCTCCA GGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508772.1
CDS	302..3928
Translation	MSDCWCFIFCKEHVRSNPLSPQHDGASREEADHQVDVSDGIRLVPDKAEATAATASDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	XM_006508709.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508709.3

ORF Insert Sequence:

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGACGATGC GGTCCCTAGT 
TCTACCTCTG AAAGCTCTGC GCTTTCCAGA AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC 
CTCAAAGCCT CTCCGACCAG TCCTGACAAA AAAGCTAAGC GGCACGAAGT AAAGAGCGAC 
CCGACTCCCT TTGGTTTGCG AGGCTGGAGC AAAACATCCC ACTCCCTGGA GGCCCCTGAG 
GAGGACGGAG GCTGGTCCAG TGCTGAGGAA CTCATCAACT CATCTGATGC TGAGGAAGAC 
GGTGGAGTGG GCCCTAAGAA GCTGGTGCCG GGTAAATACA CAGTGGTGAT GGATGATGAG 
AAAGGGGGCC CTGACACCCT TGCCATGAGG AGTGGAGATA TGGTGGAGGT GGTGGAAGAA 
GGCGCTGAGG GCCTCTGGTA TGTTCGGGAC CTGACCAGCA GCAAGGAGGG ATGGGTGCCA 
GCCAGCAGCC TATCCACCCT CCTGGGCAAA TCCAGTTCAG CCCAGTGTCT GAGCAGCTCA 
GACTCCAGCC CAGGGTCAGC CGTGCTGAGC AGCTCATCCA GCTTCAGCGA GGGCTACCCT 
GCTCCCTTCT CCGAGCTCCA GGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508710.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508773.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X6, mRNA.

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CloneID	OMu40337
Clone ID Related Accession (Same CDS sequence)	XM_006508710.3
Accession Version	XM_006508710.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508710.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACACA AACGGCCTGC CCGGATCTCT GTGGCAGAGA TGAAACTCTA CTACATGTAC 
TCTGAGTGGT GCTCCTGGCT ACGCTCTCTG GCTGAGGATG AAATCATGCA CCAGGACATC 
GTCCCGCTCT GTGCCGCTGA CATCCAGGAG CAGCTGAAGA AGCGCTTTGC TTACCTGTCT 
GGTGGGCGGG GACAGGACGG AAGCCCAGTC ATCACCTTCC CTGACTACCC AGCCTTCAGC 
GAGATCCCGG ACAAGGAGTT CCAGAATGTC ATGACCTATC TCACCAGCAT CCCCAGTTTG 
CAAGATGCTG GCATTGGATT CATCTTGGTC ATAGACCGAA GGCAGGACAA ATGGACCTCT 
GTGAAGGCAT CCGTCCTGCG GATAGCCGCA TCTTTCCCAG CCAACCTGCA GCTTGTCCTT 
GTTCTTCGGC CCACGGGCTT TTTCCAGAGG ACTCTCTCGG ACATTGCCTT CAAGTTCAAC 
AGAGATGAGT TTAAGATGAA AGTGCCGGTC ATGATGCTAA GCTCAGTGCC AGAACTACAC 
GGCTACATTG ATAAGTCGCA GCTGACCGAG GACCTCGGGG GGACCCTGGA CTACTGCCAC 
TCCAGGTGGC TGTGCCACCG CACAGCAATT GAAAGCTTCG CCCTGATGGT GAAGCAGACA 
GCCCAGATGC TGCAGGCCTT TGGGACCGAG CTGGCTGAAA CAGAGCTGCC GAATGACGTC 
CAGTCAACCA GCCTGGTGCT CAGCGCACAC ACAGAGAAGA AGGCCAAAGT GAAGGAAGAT 
CTGCAGTTAG CACTGAAGGA GGGGAACAGC ATCCTGGAGA GCCTCAGAGA GCCACTGGCT 
GAGAGCGCGG CCCACAGTGT GAACCAGGAC CAGCTGGACA ATCAGGCCAC CGTGCAGAGG 
CTCCTGGCCC AGCTGAATGA GACGGAGGCT GCCTTTGACG AATTCTGGGC AAAGCATCAG 
CAGAAGCTGG AACAGTGCCT GCAGCTGCGG CATTTTGAGC AAGGTTTCCG GGAGGTCAAA 
ACCACCTTGG ATTCCATGTC CCAGAAGATA GCTGCTTTCA CGGATGTTGG CAACAGCCTG 
GCCCACGTGC AGCACCTTCT GAAGGACTTG ACCGCCTTTG AGGAGAAGTC CAGTGTGGCT 
GTGGACAAGG CCCGCGCCCT GTCACTGGAA GGTCAGCAGC TGATTGAGAA CAGGCACTAC 
GCTGTGGACT CCATCCACCC CAAGTGTGAA GAGCTCCAGC ACCTCTGTGA CCACTTCGCC 
TCTGAGGTCA CCAGGAGGCG GGGCCTGCTC AGCAAGTCCT TGGAGCTGCA TAGCCTCCTG 
GAGACGTCCA TGAAGTGGAG CGACGAGGGG ATCTTCCTGC TGGCCTCGCA GCCTGTGGAC 
AAATGCCAGT CCCAGGATGG CGCAGAGGCG GCCCTCCAGG AGATTGAGAA GTTTCTGGAG 
ACCGGTGCAG AAAATAAGAT TCAGGAGCTC AATGAGATTT ACAAGGAATA CGAATGCATC 
CTCAACCAGG ACCTTCTGGA ACACGTGCAA AAAGTCTTTC AGAAGCAAGA AAGCACGGAG 
GAGATGTTCC ACCGCAGGCA AGCCAGCCTG AAGAAACTGG CAGCCAAGCA GACACGGCCT 
GTGCAGCCCG TGGCCCCCCG ACCAGAGGCG CTTACCAAGT CGCCCTCCCC CTCGCCAGGC 
TCCTGGCGAA GCTCTGAGAA CTCCAGCTCT GAGGGCAACG CACTCCGCAG AGGGCCCTAC 
AGGAGAGCCA AGAGCGAAAT GAGTGAACCC CGGCAGGGCC GCACCAGCTC CACAGGGGAA 
GAGGAAGAGA GCCTGGCCAT CCTGCGCAGA CACGTGATGA ATGAGCTTCT GGACACCGAG 
CGGGCCTACG TGGAGGAGCT GCTCTGTGTC TTAGAGGGCT ATGCCGCAGA GATGGATAAC 
CCCTTGATGG CACACCTCAT CTCAACAGGC CTGCAAAACA AGAAGAACAT TCTGTTTGGA 
AACATGGAGG AAATCTATCA TTTTCACAAC AGAATATTCC TGCGGGAGCT GGAGAGCTGC 
ATAGACTGCC CAGAGCTGGT GGGGAGGTGC TTTCTGGAAA GGATGGAGGA GTTCCAGATC 
TATGAGAAGT ACTGTCAGAA CAAGCCACGC TCTGAGAGCC TGTGGAGGCA GTGCTCTGAC 
TGCCCCTTCT TCCAGGAGTG TCAGAAGAAG CTGGACCACA AGCTGAGCCT GGACTCCTAC 
CTGCTGAAAC CTGTGCAGAG GATAACCAAG TACCAGCTGC TGCTCAAGGA AATGCTGAAG 
TACAGCAAGC ACTGTGAAGG GGCAGAGGAC CTGCAGGAGG CGCTGAGCTC CATCCTGGGC 
ATCCTCAAGG CTGTGAACGA CTCCATGCAT CTCATCGCCA TCACTGGCTA TGATGGAAAC 
CTTGGCGACC TGGGGAAGCT GCTGATGCAA GGTTCCTTCA GCGTGTGGAC AGACCACAAG 
AAGGGCCACA CCAAAGTGAA GGAGCTAGCC AGGTTCAAGC CCATGCAGCG GCACCTGTTT 
CTGCACGAGA AGGCCGTCCT CTTCTGCAAG AAGAGGGAGG AGAATGGGGA GGGATACGAG 
AAGGCTCCTT CCTACAGCTA CAAGCAGTCC TTGAATATGA CGGCTGTCGG CATCACAGAG 
AACGTGAAGG GTGACACCAA GAAGTTCGAG ATCTGGTACA ACGCAAGGGA GGAGGTTTAT 
ATCATTCAGG CGCCAACCCC CGAGATTAAA GCAGCGTGGG TGAATGAGAT TCGGAAGGTG 
CTGACCAGCC AGCTGCAGGC TTGCCGGGAG GCCAGCCAGC ACCGAGCCCT GGAGCAGTCC 
CACAGCCTTC CTTTGCCCAC GCCATCCAGC ACCAGTCCCA CAAAAGGGAA CACAAGAAAT 
GTCAAGAAGC TCGAAGACCG GAAGACGGAC CCGCTCAGCC TGGAAGGCTA CGTGAGCTCA 
TCGTTACCAA AGCCCCCTGA GAAGGGCAAA GACGATGCGG TCCCTAGTTC TACCTCTGAA 
AGCTCTGCGC TTTCCAGAAA GCGCTTCACG CTGCAGGGCT TGGCTAACCT CAAAGGTCAG 
AAAGCCTCTC CGACCAGTCC TGACAAAAAA GCTAAGCGGC ACGAAGTAAA GAGCGACCCG 
ACTCCCTTTG GTTTGCGAGG CTGGAGCAAA ACATCCCACT CCCTGGAGGC CCCTGAGGAG 
GACGGAGGCT GGTCCAGTGC TGAGGAACTC ATCAACTCAT CTGATGCTGA GGAAGACGGT 
GGAGTGGGCC CTAAGAAGCT GGTGCCGGGT AAATACACAG TGGTGATGGA TGATGAGAAA 
GGGGGCCCTG ACACCCTTGC CATGAGGAGT GGAGATATGG TGGAGGTGGT GGAAGAAGGC 
GCTGAGGGCC TCTGGTATGT TCGGGACCTG ACCAGCAGCA AGGAGGGATG GGTGCCAGCC 
AGCAGCCTAT CCACCCTCCT GGGCAAATCC AGTTCAGCCC AGTGTCTGAG CAGCTCAGAC 
TCCAGCCCAG GGTCAGCCGT GCTGAGCAGC TCATCCAGCT TCAGCGAGGG CTACCCTGCT 
CCCTTCTCCG AGCTCCAGGG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508773.1
CDS	114..3677
Translation	MAHKRPARISVAEMKLYYMYSEWCSWLRSLAEDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	XM_006508710.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508710.3

ORF Insert Sequence:

ATGGCACACA AACGGCCTGC CCGGATCTCT GTGGCAGAGA TGAAACTCTA CTACATGTAC 
TCTGAGTGGT GCTCCTGGCT ACGCTCTCTG GCTGAGGATG AAATCATGCA CCAGGACATC 
GTCCCGCTCT GTGCCGCTGA CATCCAGGAG CAGCTGAAGA AGCGCTTTGC TTACCTGTCT 
GGTGGGCGGG GACAGGACGG AAGCCCAGTC ATCACCTTCC CTGACTACCC AGCCTTCAGC 
GAGATCCCGG ACAAGGAGTT CCAGAATGTC ATGACCTATC TCACCAGCAT CCCCAGTTTG 
CAAGATGCTG GCATTGGATT CATCTTGGTC ATAGACCGAA GGCAGGACAA ATGGACCTCT 
GTGAAGGCAT CCGTCCTGCG GATAGCCGCA TCTTTCCCAG CCAACCTGCA GCTTGTCCTT 
GTTCTTCGGC CCACGGGCTT TTTCCAGAGG ACTCTCTCGG ACATTGCCTT CAAGTTCAAC 
AGAGATGAGT TTAAGATGAA AGTGCCGGTC ATGATGCTAA GCTCAGTGCC AGAACTACAC 
GGCTACATTG ATAAGTCGCA GCTGACCGAG GACCTCGGGG GGACCCTGGA CTACTGCCAC 
TCCAGGTGGC TGTGCCACCG CACAGCAATT GAAAGCTTCG CCCTGATGGT GAAGCAGACA 
GCCCAGATGC TGCAGGCCTT TGGGACCGAG CTGGCTGAAA CAGAGCTGCC GAATGACGTC 
CAGTCAACCA GCCTGGTGCT CAGCGCACAC ACAGAGAAGA AGGCCAAAGT GAAGGAAGAT 
CTGCAGTTAG CACTGAAGGA GGGGAACAGC ATCCTGGAGA GCCTCAGAGA GCCACTGGCT 
GAGAGCGCGG CCCACAGTGT GAACCAGGAC CAGCTGGACA ATCAGGCCAC CGTGCAGAGG 
CTCCTGGCCC AGCTGAATGA GACGGAGGCT GCCTTTGACG AATTCTGGGC AAAGCATCAG 
CAGAAGCTGG AACAGTGCCT GCAGCTGCGG CATTTTGAGC AAGGTTTCCG GGAGGTCAAA 
ACCACCTTGG ATTCCATGTC CCAGAAGATA GCTGCTTTCA CGGATGTTGG CAACAGCCTG 
GCCCACGTGC AGCACCTTCT GAAGGACTTG ACCGCCTTTG AGGAGAAGTC CAGTGTGGCT 
GTGGACAAGG CCCGCGCCCT GTCACTGGAA GGTCAGCAGC TGATTGAGAA CAGGCACTAC 
GCTGTGGACT CCATCCACCC CAAGTGTGAA GAGCTCCAGC ACCTCTGTGA CCACTTCGCC 
TCTGAGGTCA CCAGGAGGCG GGGCCTGCTC AGCAAGTCCT TGGAGCTGCA TAGCCTCCTG 
GAGACGTCCA TGAAGTGGAG CGACGAGGGG ATCTTCCTGC TGGCCTCGCA GCCTGTGGAC 
AAATGCCAGT CCCAGGATGG CGCAGAGGCG GCCCTCCAGG AGATTGAGAA GTTTCTGGAG 
ACCGGTGCAG AAAATAAGAT TCAGGAGCTC AATGAGATTT ACAAGGAATA CGAATGCATC 
CTCAACCAGG ACCTTCTGGA ACACGTGCAA AAAGTCTTTC AGAAGCAAGA AAGCACGGAG 
GAGATGTTCC ACCGCAGGCA AGCCAGCCTG AAGAAACTGG CAGCCAAGCA GACACGGCCT 
GTGCAGCCCG TGGCCCCCCG ACCAGAGGCG CTTACCAAGT CGCCCTCCCC CTCGCCAGGC 
TCCTGGCGAA GCTCTGAGAA CTCCAGCTCT GAGGGCAACG CACTCCGCAG AGGGCCCTAC 
AGGAGAGCCA AGAGCGAAAT GAGTGAACCC CGGCAGGGCC GCACCAGCTC CACAGGGGAA 
GAGGAAGAGA GCCTGGCCAT CCTGCGCAGA CACGTGATGA ATGAGCTTCT GGACACCGAG 
CGGGCCTACG TGGAGGAGCT GCTCTGTGTC TTAGAGGGCT ATGCCGCAGA GATGGATAAC 
CCCTTGATGG CACACCTCAT CTCAACAGGC CTGCAAAACA AGAAGAACAT TCTGTTTGGA 
AACATGGAGG AAATCTATCA TTTTCACAAC AGAATATTCC TGCGGGAGCT GGAGAGCTGC 
ATAGACTGCC CAGAGCTGGT GGGGAGGTGC TTTCTGGAAA GGATGGAGGA GTTCCAGATC 
TATGAGAAGT ACTGTCAGAA CAAGCCACGC TCTGAGAGCC TGTGGAGGCA GTGCTCTGAC 
TGCCCCTTCT TCCAGGAGTG TCAGAAGAAG CTGGACCACA AGCTGAGCCT GGACTCCTAC 
CTGCTGAAAC CTGTGCAGAG GATAACCAAG TACCAGCTGC TGCTCAAGGA AATGCTGAAG 
TACAGCAAGC ACTGTGAAGG GGCAGAGGAC CTGCAGGAGG CGCTGAGCTC CATCCTGGGC 
ATCCTCAAGG CTGTGAACGA CTCCATGCAT CTCATCGCCA TCACTGGCTA TGATGGAAAC 
CTTGGCGACC TGGGGAAGCT GCTGATGCAA GGTTCCTTCA GCGTGTGGAC AGACCACAAG 
AAGGGCCACA CCAAAGTGAA GGAGCTAGCC AGGTTCAAGC CCATGCAGCG GCACCTGTTT 
CTGCACGAGA AGGCCGTCCT CTTCTGCAAG AAGAGGGAGG AGAATGGGGA GGGATACGAG 
AAGGCTCCTT CCTACAGCTA CAAGCAGTCC TTGAATATGA CGGCTGTCGG CATCACAGAG 
AACGTGAAGG GTGACACCAA GAAGTTCGAG ATCTGGTACA ACGCAAGGGA GGAGGTTTAT 
ATCATTCAGG CGCCAACCCC CGAGATTAAA GCAGCGTGGG TGAATGAGAT TCGGAAGGTG 
CTGACCAGCC AGCTGCAGGC TTGCCGGGAG GCCAGCCAGC ACCGAGCCCT GGAGCAGTCC 
CACAGCCTTC CTTTGCCCAC GCCATCCAGC ACCAGTCCCA CAAAAGGGAA CACAAGAAAT 
GTCAAGAAGC TCGAAGACCG GAAGACGGAC CCGCTCAGCC TGGAAGGCTA CGTGAGCTCA 
TCGTTACCAA AGCCCCCTGA GAAGGGCAAA GACGATGCGG TCCCTAGTTC TACCTCTGAA 
AGCTCTGCGC TTTCCAGAAA GCGCTTCACG CTGCAGGGCT TGGCTAACCT CAAAGGTCAG 
AAAGCCTCTC CGACCAGTCC TGACAAAAAA GCTAAGCGGC ACGAAGTAAA GAGCGACCCG 
ACTCCCTTTG GTTTGCGAGG CTGGAGCAAA ACATCCCACT CCCTGGAGGC CCCTGAGGAG 
GACGGAGGCT GGTCCAGTGC TGAGGAACTC ATCAACTCAT CTGATGCTGA GGAAGACGGT 
GGAGTGGGCC CTAAGAAGCT GGTGCCGGGT AAATACACAG TGGTGATGGA TGATGAGAAA 
GGGGGCCCTG ACACCCTTGC CATGAGGAGT GGAGATATGG TGGAGGTGGT GGAAGAAGGC 
GCTGAGGGCC TCTGGTATGT TCGGGACCTG ACCAGCAGCA AGGAGGGATG GGTGCCAGCC 
AGCAGCCTAT CCACCCTCCT GGGCAAATCC AGTTCAGCCC AGTGTCTGAG CAGCTCAGAC 
TCCAGCCCAG GGTCAGCCGT GCTGAGCAGC TCATCCAGCT TCAGCGAGGG CTACCCTGCT 
CCCTTCTCCG AGCTCCAGGG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508711.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508774.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X7, mRNA.

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CloneID	OMu40338
Clone ID Related Accession (Same CDS sequence)	XM_006508711.3
Accession Version	XM_006508711.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508711.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAA AGGGTGCGTC CCGGGGGGCC GGGCGGCGGT TCTGGTCACT ATCAAGGAAG 
CGTCGGGGAG CACCAGGGCG CCACAAGCCA GATGAAATCA TGCACCAGGA CATCGTCCCG 
CTCTGTGCCG CTGACATCCA GGAGCAGCTG AAGAAGCGCT TTGCTTACCT GTCTGGTGGG 
CGGGGACAGG ACGGAAGCCC AGTCATCACC TTCCCTGACT ACCCAGCCTT CAGCGAGATC 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TATCTCACCA GCATCCCCAG TTTGCAAGAT 
GCTGGCATTG GATTCATCTT GGTCATAGAC CGAAGGCAGG ACAAATGGAC CTCTGTGAAG 
GCATCCGTCC TGCGGATAGC CGCATCTTTC CCAGCCAACC TGCAGCTTGT CCTTGTTCTT 
CGGCCCACGG GCTTTTTCCA GAGGACTCTC TCGGACATTG CCTTCAAGTT CAACAGAGAT 
GAGTTTAAGA TGAAAGTGCC GGTCATGATG CTAAGCTCAG TGCCAGAACT ACACGGCTAC 
ATTGATAAGT CGCAGCTGAC CGAGGACCTC GGGGGGACCC TGGACTACTG CCACTCCAGG 
TGGCTGTGCC ACCGCACAGC AATTGAAAGC TTCGCCCTGA TGGTGAAGCA GACAGCCCAG 
ATGCTGCAGG CCTTTGGGAC CGAGCTGGCT GAAACAGAGC TGCCGAATGA CGTCCAGTCA 
ACCAGCCTGG TGCTCAGCGC ACACACAGAG AAGAAGGCCA AAGTGAAGGA AGATCTGCAG 
TTAGCACTGA AGGAGGGGAA CAGCATCCTG GAGAGCCTCA GAGAGCCACT GGCTGAGAGC 
GCGGCCCACA GTGTGAACCA GGACCAGCTG GACAATCAGG CCACCGTGCA GAGGCTCCTG 
GCCCAGCTGA ATGAGACGGA GGCTGCCTTT GACGAATTCT GGGCAAAGCA TCAGCAGAAG 
CTGGAACAGT GCCTGCAGCT GCGGCATTTT GAGCAAGGTT TCCGGGAGGT CAAAACCACC 
TTGGATTCCA TGTCCCAGAA GATAGCTGCT TTCACGGATG TTGGCAACAG CCTGGCCCAC 
GTGCAGCACC TTCTGAAGGA CTTGACCGCC TTTGAGGAGA AGTCCAGTGT GGCTGTGGAC 
AAGGCCCGCG CCCTGTCACT GGAAGGTCAG CAGCTGATTG AGAACAGGCA CTACGCTGTG 
GACTCCATCC ACCCCAAGTG TGAAGAGCTC CAGCACCTCT GTGACCACTT CGCCTCTGAG 
GTCACCAGGA GGCGGGGCCT GCTCAGCAAG TCCTTGGAGC TGCATAGCCT CCTGGAGACG 
TCCATGAAGT GGAGCGACGA GGGGATCTTC CTGCTGGCCT CGCAGCCTGT GGACAAATGC 
CAGTCCCAGG ATGGCGCAGA GGCGGCCCTC CAGGAGATTG AGAAGTTTCT GGAGACCGGT 
GCAGAAAATA AGATTCAGGA GCTCAATGAG ATTTACAAGG AATACGAATG CATCCTCAAC 
CAGGACCTTC TGGAACACGT GCAAAAAGTC TTTCAGAAGC AAGAAAGCAC GGAGGAGATG 
TTCCACCGCA GGCAAGCCAG CCTGAAGAAA CTGGCAGCCA AGCAGACACG GCCTGTGCAG 
CCCGTGGCCC CCCGACCAGA GGCGCTTACC AAGTCGCCCT CCCCCTCGCC AGGCTCCTGG 
CGAAGCTCTG AGAACTCCAG CTCTGAGGGC AACGCACTCC GCAGAGGGCC CTACAGGAGA 
GCCAAGAGCG AAATGAGTGA ACCCCGGCAG GGCCGCACCA GCTCCACAGG GGAAGAGGAA 
GAGAGCCTGG CCATCCTGCG CAGACACGTG ATGAATGAGC TTCTGGACAC CGAGCGGGCC 
TACGTGGAGG AGCTGCTCTG TGTCTTAGAG GGCTATGCCG CAGAGATGGA TAACCCCTTG 
ATGGCACACC TCATCTCAAC AGGCCTGCAA AACAAGAAGA ACATTCTGTT TGGAAACATG 
GAGGAAATCT ATCATTTTCA CAACAGAATA TTCCTGCGGG AGCTGGAGAG CTGCATAGAC 
TGCCCAGAGC TGGTGGGGAG GTGCTTTCTG GAAAGGATGG AGGAGTTCCA GATCTATGAG 
AAGTACTGTC AGAACAAGCC ACGCTCTGAG AGCCTGTGGA GGCAGTGCTC TGACTGCCCC 
TTCTTCCAGG AGTGTCAGAA GAAGCTGGAC CACAAGCTGA GCCTGGACTC CTACCTGCTG 
AAACCTGTGC AGAGGATAAC CAAGTACCAG CTGCTGCTCA AGGAAATGCT GAAGTACAGC 
AAGCACTGTG AAGGGGCAGA GGACCTGCAG GAGGCGCTGA GCTCCATCCT GGGCATCCTC 
AAGGCTGTGA ACGACTCCAT GCATCTCATC GCCATCACTG GCTATGATGG AAACCTTGGC 
GACCTGGGGA AGCTGCTGAT GCAAGGTTCC TTCAGCGTGT GGACAGACCA CAAGAAGGGC 
CACACCAAAG TGAAGGAGCT AGCCAGGTTC AAGCCCATGC AGCGGCACCT GTTTCTGCAC 
GAGAAGGCCG TCCTCTTCTG CAAGAAGAGG GAGGAGAATG GGGAGGGATA CGAGAAGGCT 
CCTTCCTACA GCTACAAGCA GTCCTTGAAT ATGACGGCTG TCGGCATCAC AGAGAACGTG 
AAGGGTGACA CCAAGAAGTT CGAGATCTGG TACAACGCAA GGGAGGAGGT TTATATCATT 
CAGGCGCCAA CCCCCGAGAT TAAAGCAGCG TGGGTGAATG AGATTCGGAA GGTGCTGACC 
AGCCAGCTGC AGGCTTGCCG GGAGGCCAGC CAGCACCGAG CCCTGGAGCA GTCCCACAGC 
CTTCCTTTGC CCACGCCATC CAGCACCAGT CCCACAAAAG GGAACACAAG AAATGTCAAG 
AAGCTCGAAG ACCGGAAGAC GGACCCGCTC AGCCTGGAAG GCTACGTGAG CTCATCGTTA 
CCAAAGCCCC CTGAGAAGGG CAAAGACGAT GCGGTCCCTA GTTCTACCTC TGAAAGCTCT 
GCGCTTTCCA GAAAGCGCTT CACGCTGCAG GGCTTGGCTA ACCTCAAAGG TCAGAAAGCC 
TCTCCGACCA GTCCTGACAA AAAAGCTAAG CGGCACGAAG TAAAGAGCGA CCCGACTCCC 
TTTGGTTTGC GAGGCTGGAG CAAAACATCC CACTCCCTGG AGGCCCCTGA GGAGGACGGA 
GGCTGGTCCA GTGCTGAGGA ACTCATCAAC TCATCTGATG CTGAGGAAGA CGGTGGAGTG 
GGCCCTAAGA AGCTGGTGCC GGGTAAATAC ACAGTGGTGA TGGATGATGA GAAAGGGGGC 
CCTGACACCC TTGCCATGAG GAGTGGAGAT ATGGTGGAGG TGGTGGAAGA AGGCGCTGAG 
GGCCTCTGGT ATGTTCGGGA CCTGACCAGC AGCAAGGAGG GATGGGTGCC AGCCAGCAGC 
CTATCCACCC TCCTGGGCAA ATCCAGTTCA GCCCAGTGTC TGAGCAGCTC AGACTCCAGC 
CCAGGGTCAG CCGTGCTGAG CAGCTCATCC AGCTTCAGCG AGGGCTACCC TGCTCCCTTC 
TCCGAGCTCC AGGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508774.1
CDS	282..3839
Translation	MAEKGASRGAGRRFWSLSRKRRGAPGRHKPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	XM_006508711.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508711.3

ORF Insert Sequence:

ATGGCAGAAA AGGGTGCGTC CCGGGGGGCC GGGCGGCGGT TCTGGTCACT ATCAAGGAAG 
CGTCGGGGAG CACCAGGGCG CCACAAGCCA GATGAAATCA TGCACCAGGA CATCGTCCCG 
CTCTGTGCCG CTGACATCCA GGAGCAGCTG AAGAAGCGCT TTGCTTACCT GTCTGGTGGG 
CGGGGACAGG ACGGAAGCCC AGTCATCACC TTCCCTGACT ACCCAGCCTT CAGCGAGATC 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TATCTCACCA GCATCCCCAG TTTGCAAGAT 
GCTGGCATTG GATTCATCTT GGTCATAGAC CGAAGGCAGG ACAAATGGAC CTCTGTGAAG 
GCATCCGTCC TGCGGATAGC CGCATCTTTC CCAGCCAACC TGCAGCTTGT CCTTGTTCTT 
CGGCCCACGG GCTTTTTCCA GAGGACTCTC TCGGACATTG CCTTCAAGTT CAACAGAGAT 
GAGTTTAAGA TGAAAGTGCC GGTCATGATG CTAAGCTCAG TGCCAGAACT ACACGGCTAC 
ATTGATAAGT CGCAGCTGAC CGAGGACCTC GGGGGGACCC TGGACTACTG CCACTCCAGG 
TGGCTGTGCC ACCGCACAGC AATTGAAAGC TTCGCCCTGA TGGTGAAGCA GACAGCCCAG 
ATGCTGCAGG CCTTTGGGAC CGAGCTGGCT GAAACAGAGC TGCCGAATGA CGTCCAGTCA 
ACCAGCCTGG TGCTCAGCGC ACACACAGAG AAGAAGGCCA AAGTGAAGGA AGATCTGCAG 
TTAGCACTGA AGGAGGGGAA CAGCATCCTG GAGAGCCTCA GAGAGCCACT GGCTGAGAGC 
GCGGCCCACA GTGTGAACCA GGACCAGCTG GACAATCAGG CCACCGTGCA GAGGCTCCTG 
GCCCAGCTGA ATGAGACGGA GGCTGCCTTT GACGAATTCT GGGCAAAGCA TCAGCAGAAG 
CTGGAACAGT GCCTGCAGCT GCGGCATTTT GAGCAAGGTT TCCGGGAGGT CAAAACCACC 
TTGGATTCCA TGTCCCAGAA GATAGCTGCT TTCACGGATG TTGGCAACAG CCTGGCCCAC 
GTGCAGCACC TTCTGAAGGA CTTGACCGCC TTTGAGGAGA AGTCCAGTGT GGCTGTGGAC 
AAGGCCCGCG CCCTGTCACT GGAAGGTCAG CAGCTGATTG AGAACAGGCA CTACGCTGTG 
GACTCCATCC ACCCCAAGTG TGAAGAGCTC CAGCACCTCT GTGACCACTT CGCCTCTGAG 
GTCACCAGGA GGCGGGGCCT GCTCAGCAAG TCCTTGGAGC TGCATAGCCT CCTGGAGACG 
TCCATGAAGT GGAGCGACGA GGGGATCTTC CTGCTGGCCT CGCAGCCTGT GGACAAATGC 
CAGTCCCAGG ATGGCGCAGA GGCGGCCCTC CAGGAGATTG AGAAGTTTCT GGAGACCGGT 
GCAGAAAATA AGATTCAGGA GCTCAATGAG ATTTACAAGG AATACGAATG CATCCTCAAC 
CAGGACCTTC TGGAACACGT GCAAAAAGTC TTTCAGAAGC AAGAAAGCAC GGAGGAGATG 
TTCCACCGCA GGCAAGCCAG CCTGAAGAAA CTGGCAGCCA AGCAGACACG GCCTGTGCAG 
CCCGTGGCCC CCCGACCAGA GGCGCTTACC AAGTCGCCCT CCCCCTCGCC AGGCTCCTGG 
CGAAGCTCTG AGAACTCCAG CTCTGAGGGC AACGCACTCC GCAGAGGGCC CTACAGGAGA 
GCCAAGAGCG AAATGAGTGA ACCCCGGCAG GGCCGCACCA GCTCCACAGG GGAAGAGGAA 
GAGAGCCTGG CCATCCTGCG CAGACACGTG ATGAATGAGC TTCTGGACAC CGAGCGGGCC 
TACGTGGAGG AGCTGCTCTG TGTCTTAGAG GGCTATGCCG CAGAGATGGA TAACCCCTTG 
ATGGCACACC TCATCTCAAC AGGCCTGCAA AACAAGAAGA ACATTCTGTT TGGAAACATG 
GAGGAAATCT ATCATTTTCA CAACAGAATA TTCCTGCGGG AGCTGGAGAG CTGCATAGAC 
TGCCCAGAGC TGGTGGGGAG GTGCTTTCTG GAAAGGATGG AGGAGTTCCA GATCTATGAG 
AAGTACTGTC AGAACAAGCC ACGCTCTGAG AGCCTGTGGA GGCAGTGCTC TGACTGCCCC 
TTCTTCCAGG AGTGTCAGAA GAAGCTGGAC CACAAGCTGA GCCTGGACTC CTACCTGCTG 
AAACCTGTGC AGAGGATAAC CAAGTACCAG CTGCTGCTCA AGGAAATGCT GAAGTACAGC 
AAGCACTGTG AAGGGGCAGA GGACCTGCAG GAGGCGCTGA GCTCCATCCT GGGCATCCTC 
AAGGCTGTGA ACGACTCCAT GCATCTCATC GCCATCACTG GCTATGATGG AAACCTTGGC 
GACCTGGGGA AGCTGCTGAT GCAAGGTTCC TTCAGCGTGT GGACAGACCA CAAGAAGGGC 
CACACCAAAG TGAAGGAGCT AGCCAGGTTC AAGCCCATGC AGCGGCACCT GTTTCTGCAC 
GAGAAGGCCG TCCTCTTCTG CAAGAAGAGG GAGGAGAATG GGGAGGGATA CGAGAAGGCT 
CCTTCCTACA GCTACAAGCA GTCCTTGAAT ATGACGGCTG TCGGCATCAC AGAGAACGTG 
AAGGGTGACA CCAAGAAGTT CGAGATCTGG TACAACGCAA GGGAGGAGGT TTATATCATT 
CAGGCGCCAA CCCCCGAGAT TAAAGCAGCG TGGGTGAATG AGATTCGGAA GGTGCTGACC 
AGCCAGCTGC AGGCTTGCCG GGAGGCCAGC CAGCACCGAG CCCTGGAGCA GTCCCACAGC 
CTTCCTTTGC CCACGCCATC CAGCACCAGT CCCACAAAAG GGAACACAAG AAATGTCAAG 
AAGCTCGAAG ACCGGAAGAC GGACCCGCTC AGCCTGGAAG GCTACGTGAG CTCATCGTTA 
CCAAAGCCCC CTGAGAAGGG CAAAGACGAT GCGGTCCCTA GTTCTACCTC TGAAAGCTCT 
GCGCTTTCCA GAAAGCGCTT CACGCTGCAG GGCTTGGCTA ACCTCAAAGG TCAGAAAGCC 
TCTCCGACCA GTCCTGACAA AAAAGCTAAG CGGCACGAAG TAAAGAGCGA CCCGACTCCC 
TTTGGTTTGC GAGGCTGGAG CAAAACATCC CACTCCCTGG AGGCCCCTGA GGAGGACGGA 
GGCTGGTCCA GTGCTGAGGA ACTCATCAAC TCATCTGATG CTGAGGAAGA CGGTGGAGTG 
GGCCCTAAGA AGCTGGTGCC GGGTAAATAC ACAGTGGTGA TGGATGATGA GAAAGGGGGC 
CCTGACACCC TTGCCATGAG GAGTGGAGAT ATGGTGGAGG TGGTGGAAGA AGGCGCTGAG 
GGCCTCTGGT ATGTTCGGGA CCTGACCAGC AGCAAGGAGG GATGGGTGCC AGCCAGCAGC 
CTATCCACCC TCCTGGGCAA ATCCAGTTCA GCCCAGTGTC TGAGCAGCTC AGACTCCAGC 
CCAGGGTCAG CCGTGCTGAG CAGCTCATCC AGCTTCAGCG AGGGCTACCC TGCTCCCTTC 
TCCGAGCTCC AGGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508712.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508775.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu40339
Clone ID Related Accession (Same CDS sequence)	XM_006508712.3
Accession Version	XM_006508712.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508712.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGGC TCAGCCATTC TCACTTGGTC TCCAGCAGCG GAGGCATCCT GCAGCCTCGG 
CCTCCATCTG TGAGGAGAGC AGCAGATGAA ATCATGCACC AGGACATCGT CCCGCTCTGT 
GCCGCTGACA TCCAGGAGCA GCTGAAGAAG CGCTTTGCTT ACCTGTCTGG TGGGCGGGGA 
CAGGACGGAA GCCCAGTCAT CACCTTCCCT GACTACCCAG CCTTCAGCGA GATCCCGGAC 
AAGGAGTTCC AGAATGTCAT GACCTATCTC ACCAGCATCC CCAGTTTGCA AGATGCTGGC 
ATTGGATTCA TCTTGGTCAT AGACCGAAGG CAGGACAAAT GGACCTCTGT GAAGGCATCC 
GTCCTGCGGA TAGCCGCATC TTTCCCAGCC AACCTGCAGC TTGTCCTTGT TCTTCGGCCC 
ACGGGCTTTT TCCAGAGGAC TCTCTCGGAC ATTGCCTTCA AGTTCAACAG AGATGAGTTT 
AAGATGAAAG TGCCGGTCAT GATGCTAAGC TCAGTGCCAG AACTACACGG CTACATTGAT 
AAGTCGCAGC TGACCGAGGA CCTCGGGGGG ACCCTGGACT ACTGCCACTC CAGGTGGCTG 
TGCCACCGCA CAGCAATTGA AAGCTTCGCC CTGATGGTGA AGCAGACAGC CCAGATGCTG 
CAGGCCTTTG GGACCGAGCT GGCTGAAACA GAGCTGCCGA ATGACGTCCA GTCAACCAGC 
CTGGTGCTCA GCGCACACAC AGAGAAGAAG GCCAAAGTGA AGGAAGATCT GCAGTTAGCA 
CTGAAGGAGG GGAACAGCAT CCTGGAGAGC CTCAGAGAGC CACTGGCTGA GAGCGCGGCC 
CACAGTGTGA ACCAGGACCA GCTGGACAAT CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAATGAGA CGGAGGCTGC CTTTGACGAA TTCTGGGCAA AGCATCAGCA GAAGCTGGAA 
CAGTGCCTGC AGCTGCGGCA TTTTGAGCAA GGTTTCCGGG AGGTCAAAAC CACCTTGGAT 
TCCATGTCCC AGAAGATAGC TGCTTTCACG GATGTTGGCA ACAGCCTGGC CCACGTGCAG 
CACCTTCTGA AGGACTTGAC CGCCTTTGAG GAGAAGTCCA GTGTGGCTGT GGACAAGGCC 
CGCGCCCTGT CACTGGAAGG TCAGCAGCTG ATTGAGAACA GGCACTACGC TGTGGACTCC 
ATCCACCCCA AGTGTGAAGA GCTCCAGCAC CTCTGTGACC ACTTCGCCTC TGAGGTCACC 
AGGAGGCGGG GCCTGCTCAG CAAGTCCTTG GAGCTGCATA GCCTCCTGGA GACGTCCATG 
AAGTGGAGCG ACGAGGGGAT CTTCCTGCTG GCCTCGCAGC CTGTGGACAA ATGCCAGTCC 
CAGGATGGCG CAGAGGCGGC CCTCCAGGAG ATTGAGAAGT TTCTGGAGAC CGGTGCAGAA 
AATAAGATTC AGGAGCTCAA TGAGATTTAC AAGGAATACG AATGCATCCT CAACCAGGAC 
CTTCTGGAAC ACGTGCAAAA AGTCTTTCAG AAGCAAGAAA GCACGGAGGA GATGTTCCAC 
CGCAGGCAAG CCAGCCTGAA GAAACTGGCA GCCAAGCAGA CACGGCCTGT GCAGCCCGTG 
GCCCCCCGAC CAGAGGCGCT TACCAAGTCG CCCTCCCCCT CGCCAGGCTC CTGGCGAAGC 
TCTGAGAACT CCAGCTCTGA GGGCAACGCA CTCCGCAGAG GGCCCTACAG GAGAGCCAAG 
AGCGAAATGA GTGAACCCCG GCAGGGCCGC ACCAGCTCCA CAGGGGAAGA GGAAGAGAGC 
CTGGCCATCC TGCGCAGACA CGTGATGAAT GAGCTTCTGG ACACCGAGCG GGCCTACGTG 
GAGGAGCTGC TCTGTGTCTT AGAGGGCTAT GCCGCAGAGA TGGATAACCC CTTGATGGCA 
CACCTCATCT CAACAGGCCT GCAAAACAAG AAGAACATTC TGTTTGGAAA CATGGAGGAA 
ATCTATCATT TTCACAACAG AATATTCCTG CGGGAGCTGG AGAGCTGCAT AGACTGCCCA 
GAGCTGGTGG GGAGGTGCTT TCTGGAAAGG ATGGAGGAGT TCCAGATCTA TGAGAAGTAC 
TGTCAGAACA AGCCACGCTC TGAGAGCCTG TGGAGGCAGT GCTCTGACTG CCCCTTCTTC 
CAGGAGTGTC AGAAGAAGCT GGACCACAAG CTGAGCCTGG ACTCCTACCT GCTGAAACCT 
GTGCAGAGGA TAACCAAGTA CCAGCTGCTG CTCAAGGAAA TGCTGAAGTA CAGCAAGCAC 
TGTGAAGGGG CAGAGGACCT GCAGGAGGCG CTGAGCTCCA TCCTGGGCAT CCTCAAGGCT 
GTGAACGACT CCATGCATCT CATCGCCATC ACTGGCTATG ATGGAAACCT TGGCGACCTG 
GGGAAGCTGC TGATGCAAGG TTCCTTCAGC GTGTGGACAG ACCACAAGAA GGGCCACACC 
AAAGTGAAGG AGCTAGCCAG GTTCAAGCCC ATGCAGCGGC ACCTGTTTCT GCACGAGAAG 
GCCGTCCTCT TCTGCAAGAA GAGGGAGGAG AATGGGGAGG GATACGAGAA GGCTCCTTCC 
TACAGCTACA AGCAGTCCTT GAATATGACG GCTGTCGGCA TCACAGAGAA CGTGAAGGGT 
GACACCAAGA AGTTCGAGAT CTGGTACAAC GCAAGGGAGG AGGTTTATAT CATTCAGGCG 
CCAACCCCCG AGATTAAAGC AGCGTGGGTG AATGAGATTC GGAAGGTGCT GACCAGCCAG 
CTGCAGGCTT GCCGGGAGGC CAGCCAGCAC CGAGCCCTGG AGCAGTCCCA CAGCCTTCCT 
TTGCCCACGC CATCCAGCAC CAGTCCCACA AAAGGGAACA CAAGAAATGT CAAGAAGCTC 
GAAGACCGGA AGACGGACCC GCTCAGCCTG GAAGGCTACG TGAGCTCATC GTTACCAAAG 
CCCCCTGAGA AGGGCAAAGA CGATGCGGTC CCTAGTTCTA CCTCTGAAAG CTCTGCGCTT 
TCCAGAAAGC GCTTCACGCT GCAGGGCTTG GCTAACCTCA AAGGTCAGAA AGCCTCTCCG 
ACCAGTCCTG ACAAAAAAGC TAAGCGGCAC GAAGTAAAGA GCGACCCGAC TCCCTTTGGT 
TTGCGAGGCT GGAGCAAAAC ATCCCACTCC CTGGAGGCCC CTGAGGAGGA CGGAGGCTGG 
TCCAGTGCTG AGGAACTCAT CAACTCATCT GATGCTGAGG AAGACGGTGG AGTGGGCCCT 
AAGAAGCTGG TGCCGGGTAA ATACACAGTG GTGATGGATG ATGAGAAAGG GGGCCCTGAC 
ACCCTTGCCA TGAGGAGTGG AGATATGGTG GAGGTGGTGG AAGAAGGCGC TGAGGGCCTC 
TGGTATGTTC GGGACCTGAC CAGCAGCAAG GAGGGATGGG TGCCAGCCAG CAGCCTATCC 
ACCCTCCTGG GCAAATCCAG TTCAGCCCAG TGTCTGAGCA GCTCAGACTC CAGCCCAGGG 
TCAGCCGTGC TGAGCAGCTC ATCCAGCTTC AGCGAGGGCT ACCCTGCTCC CTTCTCCGAG 
CTCCAGGGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508775.1
CDS	88..3639
Translation	MGGLSHSHLVSSSGGILQPRPPSVRRAADEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	XM_006508712.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508712.3

ORF Insert Sequence:

ATGGGCGGGC TCAGCCATTC TCACTTGGTC TCCAGCAGCG GAGGCATCCT GCAGCCTCGG 
CCTCCATCTG TGAGGAGAGC AGCAGATGAA ATCATGCACC AGGACATCGT CCCGCTCTGT 
GCCGCTGACA TCCAGGAGCA GCTGAAGAAG CGCTTTGCTT ACCTGTCTGG TGGGCGGGGA 
CAGGACGGAA GCCCAGTCAT CACCTTCCCT GACTACCCAG CCTTCAGCGA GATCCCGGAC 
AAGGAGTTCC AGAATGTCAT GACCTATCTC ACCAGCATCC CCAGTTTGCA AGATGCTGGC 
ATTGGATTCA TCTTGGTCAT AGACCGAAGG CAGGACAAAT GGACCTCTGT GAAGGCATCC 
GTCCTGCGGA TAGCCGCATC TTTCCCAGCC AACCTGCAGC TTGTCCTTGT TCTTCGGCCC 
ACGGGCTTTT TCCAGAGGAC TCTCTCGGAC ATTGCCTTCA AGTTCAACAG AGATGAGTTT 
AAGATGAAAG TGCCGGTCAT GATGCTAAGC TCAGTGCCAG AACTACACGG CTACATTGAT 
AAGTCGCAGC TGACCGAGGA CCTCGGGGGG ACCCTGGACT ACTGCCACTC CAGGTGGCTG 
TGCCACCGCA CAGCAATTGA AAGCTTCGCC CTGATGGTGA AGCAGACAGC CCAGATGCTG 
CAGGCCTTTG GGACCGAGCT GGCTGAAACA GAGCTGCCGA ATGACGTCCA GTCAACCAGC 
CTGGTGCTCA GCGCACACAC AGAGAAGAAG GCCAAAGTGA AGGAAGATCT GCAGTTAGCA 
CTGAAGGAGG GGAACAGCAT CCTGGAGAGC CTCAGAGAGC CACTGGCTGA GAGCGCGGCC 
CACAGTGTGA ACCAGGACCA GCTGGACAAT CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAATGAGA CGGAGGCTGC CTTTGACGAA TTCTGGGCAA AGCATCAGCA GAAGCTGGAA 
CAGTGCCTGC AGCTGCGGCA TTTTGAGCAA GGTTTCCGGG AGGTCAAAAC CACCTTGGAT 
TCCATGTCCC AGAAGATAGC TGCTTTCACG GATGTTGGCA ACAGCCTGGC CCACGTGCAG 
CACCTTCTGA AGGACTTGAC CGCCTTTGAG GAGAAGTCCA GTGTGGCTGT GGACAAGGCC 
CGCGCCCTGT CACTGGAAGG TCAGCAGCTG ATTGAGAACA GGCACTACGC TGTGGACTCC 
ATCCACCCCA AGTGTGAAGA GCTCCAGCAC CTCTGTGACC ACTTCGCCTC TGAGGTCACC 
AGGAGGCGGG GCCTGCTCAG CAAGTCCTTG GAGCTGCATA GCCTCCTGGA GACGTCCATG 
AAGTGGAGCG ACGAGGGGAT CTTCCTGCTG GCCTCGCAGC CTGTGGACAA ATGCCAGTCC 
CAGGATGGCG CAGAGGCGGC CCTCCAGGAG ATTGAGAAGT TTCTGGAGAC CGGTGCAGAA 
AATAAGATTC AGGAGCTCAA TGAGATTTAC AAGGAATACG AATGCATCCT CAACCAGGAC 
CTTCTGGAAC ACGTGCAAAA AGTCTTTCAG AAGCAAGAAA GCACGGAGGA GATGTTCCAC 
CGCAGGCAAG CCAGCCTGAA GAAACTGGCA GCCAAGCAGA CACGGCCTGT GCAGCCCGTG 
GCCCCCCGAC CAGAGGCGCT TACCAAGTCG CCCTCCCCCT CGCCAGGCTC CTGGCGAAGC 
TCTGAGAACT CCAGCTCTGA GGGCAACGCA CTCCGCAGAG GGCCCTACAG GAGAGCCAAG 
AGCGAAATGA GTGAACCCCG GCAGGGCCGC ACCAGCTCCA CAGGGGAAGA GGAAGAGAGC 
CTGGCCATCC TGCGCAGACA CGTGATGAAT GAGCTTCTGG ACACCGAGCG GGCCTACGTG 
GAGGAGCTGC TCTGTGTCTT AGAGGGCTAT GCCGCAGAGA TGGATAACCC CTTGATGGCA 
CACCTCATCT CAACAGGCCT GCAAAACAAG AAGAACATTC TGTTTGGAAA CATGGAGGAA 
ATCTATCATT TTCACAACAG AATATTCCTG CGGGAGCTGG AGAGCTGCAT AGACTGCCCA 
GAGCTGGTGG GGAGGTGCTT TCTGGAAAGG ATGGAGGAGT TCCAGATCTA TGAGAAGTAC 
TGTCAGAACA AGCCACGCTC TGAGAGCCTG TGGAGGCAGT GCTCTGACTG CCCCTTCTTC 
CAGGAGTGTC AGAAGAAGCT GGACCACAAG CTGAGCCTGG ACTCCTACCT GCTGAAACCT 
GTGCAGAGGA TAACCAAGTA CCAGCTGCTG CTCAAGGAAA TGCTGAAGTA CAGCAAGCAC 
TGTGAAGGGG CAGAGGACCT GCAGGAGGCG CTGAGCTCCA TCCTGGGCAT CCTCAAGGCT 
GTGAACGACT CCATGCATCT CATCGCCATC ACTGGCTATG ATGGAAACCT TGGCGACCTG 
GGGAAGCTGC TGATGCAAGG TTCCTTCAGC GTGTGGACAG ACCACAAGAA GGGCCACACC 
AAAGTGAAGG AGCTAGCCAG GTTCAAGCCC ATGCAGCGGC ACCTGTTTCT GCACGAGAAG 
GCCGTCCTCT TCTGCAAGAA GAGGGAGGAG AATGGGGAGG GATACGAGAA GGCTCCTTCC 
TACAGCTACA AGCAGTCCTT GAATATGACG GCTGTCGGCA TCACAGAGAA CGTGAAGGGT 
GACACCAAGA AGTTCGAGAT CTGGTACAAC GCAAGGGAGG AGGTTTATAT CATTCAGGCG 
CCAACCCCCG AGATTAAAGC AGCGTGGGTG AATGAGATTC GGAAGGTGCT GACCAGCCAG 
CTGCAGGCTT GCCGGGAGGC CAGCCAGCAC CGAGCCCTGG AGCAGTCCCA CAGCCTTCCT 
TTGCCCACGC CATCCAGCAC CAGTCCCACA AAAGGGAACA CAAGAAATGT CAAGAAGCTC 
GAAGACCGGA AGACGGACCC GCTCAGCCTG GAAGGCTACG TGAGCTCATC GTTACCAAAG 
CCCCCTGAGA AGGGCAAAGA CGATGCGGTC CCTAGTTCTA CCTCTGAAAG CTCTGCGCTT 
TCCAGAAAGC GCTTCACGCT GCAGGGCTTG GCTAACCTCA AAGGTCAGAA AGCCTCTCCG 
ACCAGTCCTG ACAAAAAAGC TAAGCGGCAC GAAGTAAAGA GCGACCCGAC TCCCTTTGGT 
TTGCGAGGCT GGAGCAAAAC ATCCCACTCC CTGGAGGCCC CTGAGGAGGA CGGAGGCTGG 
TCCAGTGCTG AGGAACTCAT CAACTCATCT GATGCTGAGG AAGACGGTGG AGTGGGCCCT 
AAGAAGCTGG TGCCGGGTAA ATACACAGTG GTGATGGATG ATGAGAAAGG GGGCCCTGAC 
ACCCTTGCCA TGAGGAGTGG AGATATGGTG GAGGTGGTGG AAGAAGGCGC TGAGGGCCTC 
TGGTATGTTC GGGACCTGAC CAGCAGCAAG GAGGGATGGG TGCCAGCCAG CAGCCTATCC 
ACCCTCCTGG GCAAATCCAG TTCAGCCCAG TGTCTGAGCA GCTCAGACTC CAGCCCAGGG 
TCAGCCGTGC TGAGCAGCTC ATCCAGCTTC AGCGAGGGCT ACCCTGCTCC CTTCTCCGAG 
CTCCAGGGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508713.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508776.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X9, mRNA.

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CloneID	OMu40340
Clone ID Related Accession (Same CDS sequence)	XM_006508713.3
Accession Version	XM_006508713.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGACGATGC GGTCCCTAGT TCTACCTCTG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC CTCAAAGGTC AGAAAGCCTC TCCGACCAGT 
CCTGACAAAA AAGCTAAGCG GCACGAAGTA AAGAGCGACC CGACTCCCTT TGGTTTGCGA 
GGCTGGAGCA AAACATCCCA CTCCCTGGAG GCCCCTGAGG AGGACGGAGG CTGGTCCAGT 
GCTGAGGAAC TCATCAACTC ATCTGATGCT GAGGAAGACG GTGGAGTGGG CCCTAAGAAG 
CTGGTGCCGG GTAAATACAC AGTGGTGATG GATGATGAGA AAGGGGGCCC TGACACCCTT 
GCCATGAGGA GTGGAGATAT GGTGGAGGTG GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT 
GTTCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT ATCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC 
GTGCTGAGCA GCTCATCCAG CTTCAGCGAG GGCTACCCTG CTCCCTTCTC CGAGCTCCAG 
GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508776.1
CDS	100..3645
Translation	MRFWLRNEEMALEEMVQRLNAVSKNTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	XM_006508713.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508713.3

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGACGATGC GGTCCCTAGT TCTACCTCTG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC CTCAAAGGTC AGAAAGCCTC TCCGACCAGT 
CCTGACAAAA AAGCTAAGCG GCACGAAGTA AAGAGCGACC CGACTCCCTT TGGTTTGCGA 
GGCTGGAGCA AAACATCCCA CTCCCTGGAG GCCCCTGAGG AGGACGGAGG CTGGTCCAGT 
GCTGAGGAAC TCATCAACTC ATCTGATGCT GAGGAAGACG GTGGAGTGGG CCCTAAGAAG 
CTGGTGCCGG GTAAATACAC AGTGGTGATG GATGATGAGA AAGGGGGCCC TGACACCCTT 
GCCATGAGGA GTGGAGATAT GGTGGAGGTG GTGGAAGAAG GCGCTGAGGG CCTCTGGTAT 
GTTCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT ATCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC 
GTGCTGAGCA GCTCATCCAG CTTCAGCGAG GGCTACCCTG CTCCCTTCTC CGAGCTCCAG 
GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508714.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508777.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X10, mRNA.

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CloneID	OMu40341
Clone ID Related Accession (Same CDS sequence)	XM_006508714.3
Accession Version	XM_006508714.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508714.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGCCTCTCC GACCAGTCCT 
GACAAAAAAG CTAAGCGGCA CGAAGTAAAG AGCGACCCGA CTCCCTTTGG TTTGCGAGGC 
TGGAGCAAAA CATCCCACTC CCTGGAGGCC CCTGAGGAGG ACGGAGGCTG GTCCAGTGCT 
GAGGAACTCA TCAACTCATC TGATGCTGAG GAAGACGGTG GAGTGGGCCC TAAGAAGCTG 
GTGCCGGGTA AATACACAGT GGTGATGGAT GATGAGAAAG GGGGCCCTGA CACCCTTGCC 
ATGAGGAGTG GAGATATGGT GGAGGTGGTG GAAGAAGGCG CTGAGGGCCT CTGGTATGTT 
CGGGACCTGA CCAGCAGCAA GGAGGGATGG GTGCCAGCCA GCAGCCTATC CACCCTCCTG 
GGCAAATCCA GTTCAGCCCA GTGTCTGAGC AGCTCAGACT CCAGCCCAGG GTCAGCCGTG 
CTGAGCAGCT CATCCAGCTT CAGCGAGGGC TACCCTGCTC CCTTCTCCGA GCTCCAGGGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508777.1
CDS	302..3844
Translation	MSDCWCFIFCKEHVRSNPLSPQHDGASREEADHQVDVSDGIRLVPDKAEATAATASDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	XM_006508714.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508714.3

ORF Insert Sequence:

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGCCTCTCC GACCAGTCCT 
GACAAAAAAG CTAAGCGGCA CGAAGTAAAG AGCGACCCGA CTCCCTTTGG TTTGCGAGGC 
TGGAGCAAAA CATCCCACTC CCTGGAGGCC CCTGAGGAGG ACGGAGGCTG GTCCAGTGCT 
GAGGAACTCA TCAACTCATC TGATGCTGAG GAAGACGGTG GAGTGGGCCC TAAGAAGCTG 
GTGCCGGGTA AATACACAGT GGTGATGGAT GATGAGAAAG GGGGCCCTGA CACCCTTGCC 
ATGAGGAGTG GAGATATGGT GGAGGTGGTG GAAGAAGGCG CTGAGGGCCT CTGGTATGTT 
CGGGACCTGA CCAGCAGCAA GGAGGGATGG GTGCCAGCCA GCAGCCTATC CACCCTCCTG 
GGCAAATCCA GTTCAGCCCA GTGTCTGAGC AGCTCAGACT CCAGCCCAGG GTCAGCCGTG 
CTGAGCAGCT CATCCAGCTT CAGCGAGGGC TACCCTGCTC CCTTCTCCGA GCTCCAGGGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508715.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508778.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu40342
Clone ID Related Accession (Same CDS sequence)	XM_006508715.2
Accession Version	XM_006508715.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006508715.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTGA GCCTCATCTC CGTGCTGTCC CAGGACGTCA CAGCCCTGTG GCTCCTGCTG 
AAGACAAGTG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC CGCTGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGACA GGACGGAAGC 
CCAGTCATCA CCTTCCCTGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATC 
TTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCTGTGA AGGCATCCGT CCTGCGGATA 
GCCGCATCTT TCCCAGCCAA CCTGCAGCTT GTCCTTGTTC TTCGGCCCAC GGGCTTTTTC 
CAGAGGACTC TCTCGGACAT TGCCTTCAAG TTCAACAGAG ATGAGTTTAA GATGAAAGTG 
CCGGTCATGA TGCTAAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCGCAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCACTCCA GGTGGCTGTG CCACCGCACA 
GCAATTGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCCC AGATGCTGCA GGCCTTTGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCGAAT GACGTCCAGT CAACCAGCCT GGTGCTCAGC 
GCACACACAG AGAAGAAGGC CAAAGTGAAG GAAGATCTGC AGTTAGCACT GAAGGAGGGG 
AACAGCATCC TGGAGAGCCT CAGAGAGCCA CTGGCTGAGA GCGCGGCCCA CAGTGTGAAC 
CAGGACCAGC TGGACAATCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAGACG 
GAGGCTGCCT TTGACGAATT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAAGG TTTCCGGGAG GTCAAAACCA CCTTGGATTC CATGTCCCAG 
AAGATAGCTG CTTTCACGGA TGTTGGCAAC AGCCTGGCCC ACGTGCAGCA CCTTCTGAAG 
GACTTGACCG CCTTTGAGGA GAAGTCCAGT GTGGCTGTGG ACAAGGCCCG CGCCCTGTCA 
CTGGAAGGTC AGCAGCTGAT TGAGAACAGG CACTACGCTG TGGACTCCAT CCACCCCAAG 
TGTGAAGAGC TCCAGCACCT CTGTGACCAC TTCGCCTCTG AGGTCACCAG GAGGCGGGGC 
CTGCTCAGCA AGTCCTTGGA GCTGCATAGC CTCCTGGAGA CGTCCATGAA GTGGAGCGAC 
GAGGGGATCT TCCTGCTGGC CTCGCAGCCT GTGGACAAAT GCCAGTCCCA GGATGGCGCA 
GAGGCGGCCC TCCAGGAGAT TGAGAAGTTT CTGGAGACCG GTGCAGAAAA TAAGATTCAG 
GAGCTCAATG AGATTTACAA GGAATACGAA TGCATCCTCA ACCAGGACCT TCTGGAACAC 
GTGCAAAAAG TCTTTCAGAA GCAAGAAAGC ACGGAGGAGA TGTTCCACCG CAGGCAAGCC 
AGCCTGAAGA AACTGGCAGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCCCGACCA 
GAGGCGCTTA CCAAGTCGCC CTCCCCCTCG CCAGGCTCCT GGCGAAGCTC TGAGAACTCC 
AGCTCTGAGG GCAACGCACT CCGCAGAGGG CCCTACAGGA GAGCCAAGAG CGAAATGAGT 
GAACCCCGGC AGGGCCGCAC CAGCTCCACA GGGGAAGAGG AAGAGAGCCT GGCCATCCTG 
CGCAGACACG TGATGAATGA GCTTCTGGAC ACCGAGCGGG CCTACGTGGA GGAGCTGCTC 
TGTGTCTTAG AGGGCTATGC CGCAGAGATG GATAACCCCT TGATGGCACA CCTCATCTCA 
ACAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAAAT CTATCATTTT 
CACAACAGAA TATTCCTGCG GGAGCTGGAG AGCTGCATAG ACTGCCCAGA GCTGGTGGGG 
AGGTGCTTTC TGGAAAGGAT GGAGGAGTTC CAGATCTATG AGAAGTACTG TCAGAACAAG 
CCACGCTCTG AGAGCCTGTG GAGGCAGTGC TCTGACTGCC CCTTCTTCCA GGAGTGTCAG 
AAGAAGCTGG ACCACAAGCT GAGCCTGGAC TCCTACCTGC TGAAACCTGT GCAGAGGATA 
ACCAAGTACC AGCTGCTGCT CAAGGAAATG CTGAAGTACA GCAAGCACTG TGAAGGGGCA 
GAGGACCTGC AGGAGGCGCT GAGCTCCATC CTGGGCATCC TCAAGGCTGT GAACGACTCC 
ATGCATCTCA TCGCCATCAC TGGCTATGAT GGAAACCTTG GCGACCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGCGT GTGGACAGAC CACAAGAAGG GCCACACCAA AGTGAAGGAG 
CTAGCCAGGT TCAAGCCCAT GCAGCGGCAC CTGTTTCTGC ACGAGAAGGC CGTCCTCTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGA TACGAGAAGG CTCCTTCCTA CAGCTACAAG 
CAGTCCTTGA ATATGACGGC TGTCGGCATC ACAGAGAACG TGAAGGGTGA CACCAAGAAG 
TTCGAGATCT GGTACAACGC AAGGGAGGAG GTTTATATCA TTCAGGCGCC AACCCCCGAG 
ATTAAAGCAG CGTGGGTGAA TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCTTGC 
CGGGAGGCCA GCCAGCACCG AGCCCTGGAG CAGTCCCACA GCCTTCCTTT GCCCACGCCA 
TCCAGCACCA GTCCCACAAA AGGGAACACA AGAAATGTCA AGAAGCTCGA AGACCGGAAG 
ACGGACCCGC TCAGCCTGGA AGGCTACGTG AGCTCATCGT TACCAAAGCC CCCTGAGAAG 
GGCAAAGACG ATGCGGTCCC TAGTTCTACC TCTGAAAGCT CTGCGCTTTC CAGAAAGCGC 
TTCACGCTGC AGGGCTTGGC TAACCTCAAA GGTCAGAAAG CCTCTCCGAC CAGTCCTGAC 
AAAAAAGCTA AGCGGCACGA AGTAAAGAGC GACCCGACTC CCTTTGGTTT GCGAGGCTGG 
AGCAAAACAT CCCACTCCCT GGAGGCCCCT GAGGAGGACG GAGGCTGGTC CAGTGCTGAG 
GAACTCATCA ACTCATCTGA TGCTGAGGAA GACGGTGGAG TGGGCCCTAA GAAGCTGGTG 
CCGGGTAAAT ACACAGTGGT GATGGATGAT GAGAAAGGGG GCCCTGACAC CCTTGCCATG 
AGGAGTGGAG ATATGGTGGA GGTGGTGGAA GAAGGCGCTG AGGGCCTCTG GTATGTTCGG 
GACCTGACCA GCAGCAAGGA GGGATGGGTG CCAGCCAGCA GCCTATCCAC CCTCCTGGGC 
AAATCCAGTT CAGCCCAGTG TCTGAGCAGC TCAGACTCCA GCCCAGGGTC AGCCGTGCTG 
AGCAGCTCAT CCAGCTTCAG CGAGGGCTAC CCTGCTCCCT TCTCCGAGCT CCAGGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508778.1
CDS	330..3869
Translation	MTLSLISVLSQDVTALWLLLKTSADEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	XM_006508715.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508715.2

ORF Insert Sequence:

ATGACTTTGA GCCTCATCTC CGTGCTGTCC CAGGACGTCA CAGCCCTGTG GCTCCTGCTG 
AAGACAAGTG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC CGCTGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGACA GGACGGAAGC 
CCAGTCATCA CCTTCCCTGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATC 
TTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCTGTGA AGGCATCCGT CCTGCGGATA 
GCCGCATCTT TCCCAGCCAA CCTGCAGCTT GTCCTTGTTC TTCGGCCCAC GGGCTTTTTC 
CAGAGGACTC TCTCGGACAT TGCCTTCAAG TTCAACAGAG ATGAGTTTAA GATGAAAGTG 
CCGGTCATGA TGCTAAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCGCAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCACTCCA GGTGGCTGTG CCACCGCACA 
GCAATTGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCCC AGATGCTGCA GGCCTTTGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCGAAT GACGTCCAGT CAACCAGCCT GGTGCTCAGC 
GCACACACAG AGAAGAAGGC CAAAGTGAAG GAAGATCTGC AGTTAGCACT GAAGGAGGGG 
AACAGCATCC TGGAGAGCCT CAGAGAGCCA CTGGCTGAGA GCGCGGCCCA CAGTGTGAAC 
CAGGACCAGC TGGACAATCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAGACG 
GAGGCTGCCT TTGACGAATT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAAGG TTTCCGGGAG GTCAAAACCA CCTTGGATTC CATGTCCCAG 
AAGATAGCTG CTTTCACGGA TGTTGGCAAC AGCCTGGCCC ACGTGCAGCA CCTTCTGAAG 
GACTTGACCG CCTTTGAGGA GAAGTCCAGT GTGGCTGTGG ACAAGGCCCG CGCCCTGTCA 
CTGGAAGGTC AGCAGCTGAT TGAGAACAGG CACTACGCTG TGGACTCCAT CCACCCCAAG 
TGTGAAGAGC TCCAGCACCT CTGTGACCAC TTCGCCTCTG AGGTCACCAG GAGGCGGGGC 
CTGCTCAGCA AGTCCTTGGA GCTGCATAGC CTCCTGGAGA CGTCCATGAA GTGGAGCGAC 
GAGGGGATCT TCCTGCTGGC CTCGCAGCCT GTGGACAAAT GCCAGTCCCA GGATGGCGCA 
GAGGCGGCCC TCCAGGAGAT TGAGAAGTTT CTGGAGACCG GTGCAGAAAA TAAGATTCAG 
GAGCTCAATG AGATTTACAA GGAATACGAA TGCATCCTCA ACCAGGACCT TCTGGAACAC 
GTGCAAAAAG TCTTTCAGAA GCAAGAAAGC ACGGAGGAGA TGTTCCACCG CAGGCAAGCC 
AGCCTGAAGA AACTGGCAGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCCCGACCA 
GAGGCGCTTA CCAAGTCGCC CTCCCCCTCG CCAGGCTCCT GGCGAAGCTC TGAGAACTCC 
AGCTCTGAGG GCAACGCACT CCGCAGAGGG CCCTACAGGA GAGCCAAGAG CGAAATGAGT 
GAACCCCGGC AGGGCCGCAC CAGCTCCACA GGGGAAGAGG AAGAGAGCCT GGCCATCCTG 
CGCAGACACG TGATGAATGA GCTTCTGGAC ACCGAGCGGG CCTACGTGGA GGAGCTGCTC 
TGTGTCTTAG AGGGCTATGC CGCAGAGATG GATAACCCCT TGATGGCACA CCTCATCTCA 
ACAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAAAT CTATCATTTT 
CACAACAGAA TATTCCTGCG GGAGCTGGAG AGCTGCATAG ACTGCCCAGA GCTGGTGGGG 
AGGTGCTTTC TGGAAAGGAT GGAGGAGTTC CAGATCTATG AGAAGTACTG TCAGAACAAG 
CCACGCTCTG AGAGCCTGTG GAGGCAGTGC TCTGACTGCC CCTTCTTCCA GGAGTGTCAG 
AAGAAGCTGG ACCACAAGCT GAGCCTGGAC TCCTACCTGC TGAAACCTGT GCAGAGGATA 
ACCAAGTACC AGCTGCTGCT CAAGGAAATG CTGAAGTACA GCAAGCACTG TGAAGGGGCA 
GAGGACCTGC AGGAGGCGCT GAGCTCCATC CTGGGCATCC TCAAGGCTGT GAACGACTCC 
ATGCATCTCA TCGCCATCAC TGGCTATGAT GGAAACCTTG GCGACCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGCGT GTGGACAGAC CACAAGAAGG GCCACACCAA AGTGAAGGAG 
CTAGCCAGGT TCAAGCCCAT GCAGCGGCAC CTGTTTCTGC ACGAGAAGGC CGTCCTCTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGA TACGAGAAGG CTCCTTCCTA CAGCTACAAG 
CAGTCCTTGA ATATGACGGC TGTCGGCATC ACAGAGAACG TGAAGGGTGA CACCAAGAAG 
TTCGAGATCT GGTACAACGC AAGGGAGGAG GTTTATATCA TTCAGGCGCC AACCCCCGAG 
ATTAAAGCAG CGTGGGTGAA TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCTTGC 
CGGGAGGCCA GCCAGCACCG AGCCCTGGAG CAGTCCCACA GCCTTCCTTT GCCCACGCCA 
TCCAGCACCA GTCCCACAAA AGGGAACACA AGAAATGTCA AGAAGCTCGA AGACCGGAAG 
ACGGACCCGC TCAGCCTGGA AGGCTACGTG AGCTCATCGT TACCAAAGCC CCCTGAGAAG 
GGCAAAGACG ATGCGGTCCC TAGTTCTACC TCTGAAAGCT CTGCGCTTTC CAGAAAGCGC 
TTCACGCTGC AGGGCTTGGC TAACCTCAAA GGTCAGAAAG CCTCTCCGAC CAGTCCTGAC 
AAAAAAGCTA AGCGGCACGA AGTAAAGAGC GACCCGACTC CCTTTGGTTT GCGAGGCTGG 
AGCAAAACAT CCCACTCCCT GGAGGCCCCT GAGGAGGACG GAGGCTGGTC CAGTGCTGAG 
GAACTCATCA ACTCATCTGA TGCTGAGGAA GACGGTGGAG TGGGCCCTAA GAAGCTGGTG 
CCGGGTAAAT ACACAGTGGT GATGGATGAT GAGAAAGGGG GCCCTGACAC CCTTGCCATG 
AGGAGTGGAG ATATGGTGGA GGTGGTGGAA GAAGGCGCTG AGGGCCTCTG GTATGTTCGG 
GACCTGACCA GCAGCAAGGA GGGATGGGTG CCAGCCAGCA GCCTATCCAC CCTCCTGGGC 
AAATCCAGTT CAGCCCAGTG TCTGAGCAGC TCAGACTCCA GCCCAGGGTC AGCCGTGCTG 
AGCAGCTCAT CCAGCTTCAG CGAGGGCTAC CCTGCTCCCT TCTCCGAGCT CCAGGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508716.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508779.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X12, mRNA.

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CloneID	OMu40343
Clone ID Related Accession (Same CDS sequence)	XM_006508716.3
Accession Version	XM_006508716.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508716.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACCTC GTGGAGAGCG CGCTCTAGGC TGCTGTGGAC TCTGCACCTG CCCTGCAGCG 
GCGGGAGATG AAATCATGCA CCAGGACATC GTCCCGCTCT GTGCCGCTGA CATCCAGGAG 
CAGCTGAAGA AGCGCTTTGC TTACCTGTCT GGTGGGCGGG GACAGGACGG AAGCCCAGTC 
ATCACCTTCC CTGACTACCC AGCCTTCAGC GAGATCCCGG ACAAGGAGTT CCAGAATGTC 
ATGACCTATC TCACCAGCAT CCCCAGTTTG CAAGATGCTG GCATTGGATT CATCTTGGTC 
ATAGACCGAA GGCAGGACAA ATGGACCTCT GTGAAGGCAT CCGTCCTGCG GATAGCCGCA 
TCTTTCCCAG CCAACCTGCA GCTTGTCCTT GTTCTTCGGC CCACGGGCTT TTTCCAGAGG 
ACTCTCTCGG ACATTGCCTT CAAGTTCAAC AGAGATGAGT TTAAGATGAA AGTGCCGGTC 
ATGATGCTAA GCTCAGTGCC AGAACTACAC GGCTACATTG ATAAGTCGCA GCTGACCGAG 
GACCTCGGGG GGACCCTGGA CTACTGCCAC TCCAGGTGGC TGTGCCACCG CACAGCAATT 
GAAAGCTTCG CCCTGATGGT GAAGCAGACA GCCCAGATGC TGCAGGCCTT TGGGACCGAG 
CTGGCTGAAA CAGAGCTGCC GAATGACGTC CAGTCAACCA GCCTGGTGCT CAGCGCACAC 
ACAGAGAAGA AGGCCAAAGT GAAGGAAGAT CTGCAGTTAG CACTGAAGGA GGGGAACAGC 
ATCCTGGAGA GCCTCAGAGA GCCACTGGCT GAGAGCGCGG CCCACAGTGT GAACCAGGAC 
CAGCTGGACA ATCAGGCCAC CGTGCAGAGG CTCCTGGCCC AGCTGAATGA GACGGAGGCT 
GCCTTTGACG AATTCTGGGC AAAGCATCAG CAGAAGCTGG AACAGTGCCT GCAGCTGCGG 
CATTTTGAGC AAGGTTTCCG GGAGGTCAAA ACCACCTTGG ATTCCATGTC CCAGAAGATA 
GCTGCTTTCA CGGATGTTGG CAACAGCCTG GCCCACGTGC AGCACCTTCT GAAGGACTTG 
ACCGCCTTTG AGGAGAAGTC CAGTGTGGCT GTGGACAAGG CCCGCGCCCT GTCACTGGAA 
GGTCAGCAGC TGATTGAGAA CAGGCACTAC GCTGTGGACT CCATCCACCC CAAGTGTGAA 
GAGCTCCAGC ACCTCTGTGA CCACTTCGCC TCTGAGGTCA CCAGGAGGCG GGGCCTGCTC 
AGCAAGTCCT TGGAGCTGCA TAGCCTCCTG GAGACGTCCA TGAAGTGGAG CGACGAGGGG 
ATCTTCCTGC TGGCCTCGCA GCCTGTGGAC AAATGCCAGT CCCAGGATGG CGCAGAGGCG 
GCCCTCCAGG AGATTGAGAA GTTTCTGGAG ACCGGTGCAG AAAATAAGAT TCAGGAGCTC 
AATGAGATTT ACAAGGAATA CGAATGCATC CTCAACCAGG ACCTTCTGGA ACACGTGCAA 
AAAGTCTTTC AGAAGCAAGA AAGCACGGAG GAGATGTTCC ACCGCAGGCA AGCCAGCCTG 
AAGAAACTGG CAGCCAAGCA GACACGGCCT GTGCAGCCCG TGGCCCCCCG ACCAGAGGCG 
CTTACCAAGT CGCCCTCCCC CTCGCCAGGC TCCTGGCGAA GCTCTGAGAA CTCCAGCTCT 
GAGGGCAACG CACTCCGCAG AGGGCCCTAC AGGAGAGCCA AGAGCGAAAT GAGTGAACCC 
CGGCAGGGCC GCACCAGCTC CACAGGGGAA GAGGAAGAGA GCCTGGCCAT CCTGCGCAGA 
CACGTGATGA ATGAGCTTCT GGACACCGAG CGGGCCTACG TGGAGGAGCT GCTCTGTGTC 
TTAGAGGGCT ATGCCGCAGA GATGGATAAC CCCTTGATGG CACACCTCAT CTCAACAGGC 
CTGCAAAACA AGAAGAACAT TCTGTTTGGA AACATGGAGG AAATCTATCA TTTTCACAAC 
AGAATATTCC TGCGGGAGCT GGAGAGCTGC ATAGACTGCC CAGAGCTGGT GGGGAGGTGC 
TTTCTGGAAA GGATGGAGGA GTTCCAGATC TATGAGAAGT ACTGTCAGAA CAAGCCACGC 
TCTGAGAGCC TGTGGAGGCA GTGCTCTGAC TGCCCCTTCT TCCAGGAGTG TCAGAAGAAG 
CTGGACCACA AGCTGAGCCT GGACTCCTAC CTGCTGAAAC CTGTGCAGAG GATAACCAAG 
TACCAGCTGC TGCTCAAGGA AATGCTGAAG TACAGCAAGC ACTGTGAAGG GGCAGAGGAC 
CTGCAGGAGG CGCTGAGCTC CATCCTGGGC ATCCTCAAGG CTGTGAACGA CTCCATGCAT 
CTCATCGCCA TCACTGGCTA TGATGGAAAC CTTGGCGACC TGGGGAAGCT GCTGATGCAA 
GGTTCCTTCA GCGTGTGGAC AGACCACAAG AAGGGCCACA CCAAAGTGAA GGAGCTAGCC 
AGGTTCAAGC CCATGCAGCG GCACCTGTTT CTGCACGAGA AGGCCGTCCT CTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGATACGAG AAGGCTCCTT CCTACAGCTA CAAGCAGTCC 
TTGAATATGA CGGCTGTCGG CATCACAGAG AACGTGAAGG GTGACACCAA GAAGTTCGAG 
ATCTGGTACA ACGCAAGGGA GGAGGTTTAT ATCATTCAGG CGCCAACCCC CGAGATTAAA 
GCAGCGTGGG TGAATGAGAT TCGGAAGGTG CTGACCAGCC AGCTGCAGGC TTGCCGGGAG 
GCCAGCCAGC ACCGAGCCCT GGAGCAGTCC CACAGCCTTC CTTTGCCCAC GCCATCCAGC 
ACCAGTCCCA CAAAAGGGAA CACAAGAAAT GTCAAGAAGC TCGAAGACCG GAAGACGGAC 
CCGCTCAGCC TGGAAGGCTA CGTGAGCTCA TCGTTACCAA AGCCCCCTGA GAAGGGCAAA 
GACGATGCGG TCCCTAGTTC TACCTCTGAA AGCTCTGCGC TTTCCAGAAA GCGCTTCACG 
CTGCAGGGCT TGGCTAACCT CAAAGGTCAG AAAGCCTCTC CGACCAGTCC TGACAAAAAA 
GCTAAGCGGC ACGAAGTAAA GAGCGACCCG ACTCCCTTTG GTTTGCGAGG CTGGAGCAAA 
ACATCCCACT CCCTGGAGGC CCCTGAGGAG GACGGAGGCT GGTCCAGTGC TGAGGAACTC 
ATCAACTCAT CTGATGCTGA GGAAGACGGT GGAGTGGGCC CTAAGAAGCT GGTGCCGGGT 
AAATACACAG TGGTGATGGA TGATGAGAAA GGGGGCCCTG ACACCCTTGC CATGAGGAGT 
GGAGATATGG TGGAGGTGGT GGAAGAAGGC GCTGAGGGCC TCTGGTATGT TCGGGACCTG 
ACCAGCAGCA AGGAGGGATG GGTGCCAGCC AGCAGCCTAT CCACCCTCCT GGGCAAATCC 
AGTTCAGCCC AGTGTCTGAG CAGCTCAGAC TCCAGCCCAG GGTCAGCCGT GCTGAGCAGC 
TCATCCAGCT TCAGCGAGGG CTACCCTGCT CCCTTCTCCG AGCTCCAGGG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508779.1
CDS	24..3557
Translation	MRPRGERALGCCGLCTCPAAAGDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	XM_006508716.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508716.3

ORF Insert Sequence:

ATGAGACCTC GTGGAGAGCG CGCTCTAGGC TGCTGTGGAC TCTGCACCTG CCCTGCAGCG 
GCGGGAGATG AAATCATGCA CCAGGACATC GTCCCGCTCT GTGCCGCTGA CATCCAGGAG 
CAGCTGAAGA AGCGCTTTGC TTACCTGTCT GGTGGGCGGG GACAGGACGG AAGCCCAGTC 
ATCACCTTCC CTGACTACCC AGCCTTCAGC GAGATCCCGG ACAAGGAGTT CCAGAATGTC 
ATGACCTATC TCACCAGCAT CCCCAGTTTG CAAGATGCTG GCATTGGATT CATCTTGGTC 
ATAGACCGAA GGCAGGACAA ATGGACCTCT GTGAAGGCAT CCGTCCTGCG GATAGCCGCA 
TCTTTCCCAG CCAACCTGCA GCTTGTCCTT GTTCTTCGGC CCACGGGCTT TTTCCAGAGG 
ACTCTCTCGG ACATTGCCTT CAAGTTCAAC AGAGATGAGT TTAAGATGAA AGTGCCGGTC 
ATGATGCTAA GCTCAGTGCC AGAACTACAC GGCTACATTG ATAAGTCGCA GCTGACCGAG 
GACCTCGGGG GGACCCTGGA CTACTGCCAC TCCAGGTGGC TGTGCCACCG CACAGCAATT 
GAAAGCTTCG CCCTGATGGT GAAGCAGACA GCCCAGATGC TGCAGGCCTT TGGGACCGAG 
CTGGCTGAAA CAGAGCTGCC GAATGACGTC CAGTCAACCA GCCTGGTGCT CAGCGCACAC 
ACAGAGAAGA AGGCCAAAGT GAAGGAAGAT CTGCAGTTAG CACTGAAGGA GGGGAACAGC 
ATCCTGGAGA GCCTCAGAGA GCCACTGGCT GAGAGCGCGG CCCACAGTGT GAACCAGGAC 
CAGCTGGACA ATCAGGCCAC CGTGCAGAGG CTCCTGGCCC AGCTGAATGA GACGGAGGCT 
GCCTTTGACG AATTCTGGGC AAAGCATCAG CAGAAGCTGG AACAGTGCCT GCAGCTGCGG 
CATTTTGAGC AAGGTTTCCG GGAGGTCAAA ACCACCTTGG ATTCCATGTC CCAGAAGATA 
GCTGCTTTCA CGGATGTTGG CAACAGCCTG GCCCACGTGC AGCACCTTCT GAAGGACTTG 
ACCGCCTTTG AGGAGAAGTC CAGTGTGGCT GTGGACAAGG CCCGCGCCCT GTCACTGGAA 
GGTCAGCAGC TGATTGAGAA CAGGCACTAC GCTGTGGACT CCATCCACCC CAAGTGTGAA 
GAGCTCCAGC ACCTCTGTGA CCACTTCGCC TCTGAGGTCA CCAGGAGGCG GGGCCTGCTC 
AGCAAGTCCT TGGAGCTGCA TAGCCTCCTG GAGACGTCCA TGAAGTGGAG CGACGAGGGG 
ATCTTCCTGC TGGCCTCGCA GCCTGTGGAC AAATGCCAGT CCCAGGATGG CGCAGAGGCG 
GCCCTCCAGG AGATTGAGAA GTTTCTGGAG ACCGGTGCAG AAAATAAGAT TCAGGAGCTC 
AATGAGATTT ACAAGGAATA CGAATGCATC CTCAACCAGG ACCTTCTGGA ACACGTGCAA 
AAAGTCTTTC AGAAGCAAGA AAGCACGGAG GAGATGTTCC ACCGCAGGCA AGCCAGCCTG 
AAGAAACTGG CAGCCAAGCA GACACGGCCT GTGCAGCCCG TGGCCCCCCG ACCAGAGGCG 
CTTACCAAGT CGCCCTCCCC CTCGCCAGGC TCCTGGCGAA GCTCTGAGAA CTCCAGCTCT 
GAGGGCAACG CACTCCGCAG AGGGCCCTAC AGGAGAGCCA AGAGCGAAAT GAGTGAACCC 
CGGCAGGGCC GCACCAGCTC CACAGGGGAA GAGGAAGAGA GCCTGGCCAT CCTGCGCAGA 
CACGTGATGA ATGAGCTTCT GGACACCGAG CGGGCCTACG TGGAGGAGCT GCTCTGTGTC 
TTAGAGGGCT ATGCCGCAGA GATGGATAAC CCCTTGATGG CACACCTCAT CTCAACAGGC 
CTGCAAAACA AGAAGAACAT TCTGTTTGGA AACATGGAGG AAATCTATCA TTTTCACAAC 
AGAATATTCC TGCGGGAGCT GGAGAGCTGC ATAGACTGCC CAGAGCTGGT GGGGAGGTGC 
TTTCTGGAAA GGATGGAGGA GTTCCAGATC TATGAGAAGT ACTGTCAGAA CAAGCCACGC 
TCTGAGAGCC TGTGGAGGCA GTGCTCTGAC TGCCCCTTCT TCCAGGAGTG TCAGAAGAAG 
CTGGACCACA AGCTGAGCCT GGACTCCTAC CTGCTGAAAC CTGTGCAGAG GATAACCAAG 
TACCAGCTGC TGCTCAAGGA AATGCTGAAG TACAGCAAGC ACTGTGAAGG GGCAGAGGAC 
CTGCAGGAGG CGCTGAGCTC CATCCTGGGC ATCCTCAAGG CTGTGAACGA CTCCATGCAT 
CTCATCGCCA TCACTGGCTA TGATGGAAAC CTTGGCGACC TGGGGAAGCT GCTGATGCAA 
GGTTCCTTCA GCGTGTGGAC AGACCACAAG AAGGGCCACA CCAAAGTGAA GGAGCTAGCC 
AGGTTCAAGC CCATGCAGCG GCACCTGTTT CTGCACGAGA AGGCCGTCCT CTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGATACGAG AAGGCTCCTT CCTACAGCTA CAAGCAGTCC 
TTGAATATGA CGGCTGTCGG CATCACAGAG AACGTGAAGG GTGACACCAA GAAGTTCGAG 
ATCTGGTACA ACGCAAGGGA GGAGGTTTAT ATCATTCAGG CGCCAACCCC CGAGATTAAA 
GCAGCGTGGG TGAATGAGAT TCGGAAGGTG CTGACCAGCC AGCTGCAGGC TTGCCGGGAG 
GCCAGCCAGC ACCGAGCCCT GGAGCAGTCC CACAGCCTTC CTTTGCCCAC GCCATCCAGC 
ACCAGTCCCA CAAAAGGGAA CACAAGAAAT GTCAAGAAGC TCGAAGACCG GAAGACGGAC 
CCGCTCAGCC TGGAAGGCTA CGTGAGCTCA TCGTTACCAA AGCCCCCTGA GAAGGGCAAA 
GACGATGCGG TCCCTAGTTC TACCTCTGAA AGCTCTGCGC TTTCCAGAAA GCGCTTCACG 
CTGCAGGGCT TGGCTAACCT CAAAGGTCAG AAAGCCTCTC CGACCAGTCC TGACAAAAAA 
GCTAAGCGGC ACGAAGTAAA GAGCGACCCG ACTCCCTTTG GTTTGCGAGG CTGGAGCAAA 
ACATCCCACT CCCTGGAGGC CCCTGAGGAG GACGGAGGCT GGTCCAGTGC TGAGGAACTC 
ATCAACTCAT CTGATGCTGA GGAAGACGGT GGAGTGGGCC CTAAGAAGCT GGTGCCGGGT 
AAATACACAG TGGTGATGGA TGATGAGAAA GGGGGCCCTG ACACCCTTGC CATGAGGAGT 
GGAGATATGG TGGAGGTGGT GGAAGAAGGC GCTGAGGGCC TCTGGTATGT TCGGGACCTG 
ACCAGCAGCA AGGAGGGATG GGTGCCAGCC AGCAGCCTAT CCACCCTCCT GGGCAAATCC 
AGTTCAGCCC AGTGTCTGAG CAGCTCAGAC TCCAGCCCAG GGTCAGCCGT GCTGAGCAGC 
TCATCCAGCT TCAGCGAGGG CTACCCTGCT CCCTTCTCCG AGCTCCAGGG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508717.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508780.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu40344
Clone ID Related Accession (Same CDS sequence)	XM_006508717.3
Accession Version	XM_006508717.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508717.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGACGATGC GGTCCCTAGT TCTACCTCTG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC CTCAAAGCCT CTCCGACCAG TCCTGACAAA 
AAAGCTAAGC GGCACGAAGT AAAGAGCGAC CCGACTCCCT TTGGTTTGCG AGGCTGGAGC 
AAAACATCCC ACTCCCTGGA GGCCCCTGAG GAGGACGGAG GCTGGTCCAG TGCTGAGGAA 
CTCATCAACT CATCTGATGC TGAGGAAGAC GGTGGAGTGG GCCCTAAGAA GCTGGTGCCG 
GGTAAATACA CAGTGGTGAT GGATGATGAG AAAGGGGGCC CTGACACCCT TGCCATGAGG 
AGTGGAGATA TGGTGGAGGT GGTGGAAGAA GGCGCTGAGG GCCTCTGGTA TGTTCGGGAC 
CTGACCAGCA GCAAGGAGGG ATGGGTGCCA GCCAGCAGCC TATCCACCCT CCTGGGCAAA 
TCCAGTTCAG CCCAGTGTCT GAGCAGCTCA GGTAAGATCC ACTGTGCGCG CCAGCTGTGC 
CCTGAGCCTG CCGAGATCCT CAGCCCAGAG CCCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508780.1
CDS	100..3618
Translation	MRFWLRNEEMALEEMVQRLNAVSKNTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGKIHCARQLCPEPAEILSPEPV

Target ORF information:

RefSeq Version	XM_006508717.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508717.3

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGACGATGC GGTCCCTAGT TCTACCTCTG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC CTCAAAGCCT CTCCGACCAG TCCTGACAAA 
AAAGCTAAGC GGCACGAAGT AAAGAGCGAC CCGACTCCCT TTGGTTTGCG AGGCTGGAGC 
AAAACATCCC ACTCCCTGGA GGCCCCTGAG GAGGACGGAG GCTGGTCCAG TGCTGAGGAA 
CTCATCAACT CATCTGATGC TGAGGAAGAC GGTGGAGTGG GCCCTAAGAA GCTGGTGCCG 
GGTAAATACA CAGTGGTGAT GGATGATGAG AAAGGGGGCC CTGACACCCT TGCCATGAGG 
AGTGGAGATA TGGTGGAGGT GGTGGAAGAA GGCGCTGAGG GCCTCTGGTA TGTTCGGGAC 
CTGACCAGCA GCAAGGAGGG ATGGGTGCCA GCCAGCAGCC TATCCACCCT CCTGGGCAAA 
TCCAGTTCAG CCCAGTGTCT GAGCAGCTCA GGTAAGATCC ACTGTGCGCG CCAGCTGTGC 
CCTGAGCCTG CCGAGATCCT CAGCCCAGAG CCCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508718.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508781.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X14, mRNA.

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CloneID	OMu40345
Clone ID Related Accession (Same CDS sequence)	XM_006508718.3
Accession Version	XM_006508718.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508718.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGGCTGGAG CAAAACATCC 
CACTCCCTGG AGGCCCCTGA GGAGGACGGA GGCTGGTCCA GTGCTGAGGA ACTCATCAAC 
TCATCTGATG CTGAGGAAGA CGGTGGAGTG GGCCCTAAGA AGCTGGTGCC GGGTAAATAC 
ACAGTGGTGA TGGATGATGA GAAAGGGGGC CCTGACACCC TTGCCATGAG GAGTGGAGAT 
ATGGTGGAGG TGGTGGAAGA AGGCGCTGAG GGCCTCTGGT ATGTTCGGGA CCTGACCAGC 
AGCAAGGAGG GATGGGTGCC AGCCAGCAGC CTATCCACCC TCCTGGGCAA ATCCAGTTCA 
GCCCAGTGTC TGAGCAGCTC AGACTCCAGC CCAGGGTCAG CCGTGCTGAG CAGCTCATCC 
AGCTTCAGCG AGGGCTACCC TGCTCCCTTC TCCGAGCTCC AGGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508781.1
CDS	301..3768
Translation	MSDCWCFIFCKEHVRSNPLSPQHDGASREEADHQVDVSDGIRLVPDKAEATAATASDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	XM_006508718.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508718.3

ORF Insert Sequence:

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGGCTGGAG CAAAACATCC 
CACTCCCTGG AGGCCCCTGA GGAGGACGGA GGCTGGTCCA GTGCTGAGGA ACTCATCAAC 
TCATCTGATG CTGAGGAAGA CGGTGGAGTG GGCCCTAAGA AGCTGGTGCC GGGTAAATAC 
ACAGTGGTGA TGGATGATGA GAAAGGGGGC CCTGACACCC TTGCCATGAG GAGTGGAGAT 
ATGGTGGAGG TGGTGGAAGA AGGCGCTGAG GGCCTCTGGT ATGTTCGGGA CCTGACCAGC 
AGCAAGGAGG GATGGGTGCC AGCCAGCAGC CTATCCACCC TCCTGGGCAA ATCCAGTTCA 
GCCCAGTGTC TGAGCAGCTC AGACTCCAGC CCAGGGTCAG CCGTGCTGAG CAGCTCATCC 
AGCTTCAGCG AGGGCTACCC TGCTCCCTTC TCCGAGCTCC AGGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508719.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508782.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X15, mRNA.

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CloneID	OMu40346
Clone ID Related Accession (Same CDS sequence)	XM_006508719.3
Accession Version	XM_006508719.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508719.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGGCTGGAG CAAAACATCC 
CACTCCCTGG AGGCCCCTGA GGAGGACGGA GGCTGGTCCA GTGCTGAGGA ACTCATCAAC 
TCATCTGATG CTGAGGAAGA CGGTGGAGTG GGCCCTAAGA AGCTGGTGCC GGGTAAATAC 
ACAGTGGTGA TGGATGATGA GAAAGGGGGC CCTGACACCC TTGCCATGAG GAGTGGAGAT 
ATGGTGGAGG TGGTGGAAGA AGGCGCTGAG GGCCTCTGGT ATGTTCGGGA CCTGACCAGC 
AGCAAGGAGG GATGGGTGCC AGCCAGCAGC CTATCCACCC TCCTGGGCAA ATCCAGTTCA 
GCCCAGTGTC TGAGCAGCTC AGGTGTGCAG AGCAAAGGGC CAGAGCCGAA GCCTGTCTCC 
ACTGGTTTCC CGCAGACTCC AGCCCAGGGT CAGCCGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508782.1
CDS	301..3762
Translation	MSDCWCFIFCKEHVRSNPLSPQHDGASREEADHQVDVSDGIRLVPDKAEATAATASDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGVQSKGPEPKPVSTGFPQTPAQGQPC

Target ORF information:

RefSeq Version	XM_006508719.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508719.3

ORF Insert Sequence:

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGTCC CACAAAAGGG 
AACACAAGAA ATGTCAAGAA GCTCGAAGAC CGGAAGACGG ACCCGCTCAG CCTGGAAGGC 
TACGTGAGCT CATCGTTACC AAAGCCCCCT GAGAAGGGCA AAGGCTGGAG CAAAACATCC 
CACTCCCTGG AGGCCCCTGA GGAGGACGGA GGCTGGTCCA GTGCTGAGGA ACTCATCAAC 
TCATCTGATG CTGAGGAAGA CGGTGGAGTG GGCCCTAAGA AGCTGGTGCC GGGTAAATAC 
ACAGTGGTGA TGGATGATGA GAAAGGGGGC CCTGACACCC TTGCCATGAG GAGTGGAGAT 
ATGGTGGAGG TGGTGGAAGA AGGCGCTGAG GGCCTCTGGT ATGTTCGGGA CCTGACCAGC 
AGCAAGGAGG GATGGGTGCC AGCCAGCAGC CTATCCACCC TCCTGGGCAA ATCCAGTTCA 
GCCCAGTGTC TGAGCAGCTC AGGTGTGCAG AGCAAAGGGC CAGAGCCGAA GCCTGTCTCC 
ACTGGTTTCC CGCAGACTCC AGCCCAGGGT CAGCCGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508720.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508783.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OMu40347
Clone ID Related Accession (Same CDS sequence)	XM_006508720.3
Accession Version	XM_006508720.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGGCTGGAG CAAAACATCC CACTCCCTGG AGGCCCCTGA GGAGGACGGA 
GGCTGGTCCA GTGCTGAGGA ACTCATCAAC TCATCTGATG CTGAGGAAGA CGGTGGAGTG 
GGCCCTAAGA AGCTGGTGCC GGGTAAATAC ACAGTGGTGA TGGATGATGA GAAAGGGGGC 
CCTGACACCC TTGCCATGAG GAGTGGAGAT ATGGTGGAGG TGGTGGAAGA AGGCGCTGAG 
GGCCTCTGGT ATGTTCGGGA CCTGACCAGC AGCAAGGAGG GATGGGTGCC AGCCAGCAGC 
CTATCCACCC TCCTGGGCAA ATCCAGTTCA GCCCAGTGTC TGAGCAGCTC AGACTCCAGC 
CCAGGGTCAG CCGTGCTGAG CAGCTCATCC AGCTTCAGCG AGGGCTACCC TGCTCCCTTC 
TCCGAGCTCC AGGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508783.1
CDS	100..3477
Translation	MRFWLRNEEMALEEMVQRLNAVSKNTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	XM_006508720.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508720.3

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCTGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATCTTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CTGTGAAGGC ATCCGTCCTG 
CGGATAGCCG CATCTTTCCC AGCCAACCTG CAGCTTGTCC TTGTTCTTCG GCCCACGGGC 
TTTTTCCAGA GGACTCTCTC GGACATTGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTGCCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCG 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCAA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCTGA AACAGAGCTG CCGAATGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCCAAA GTGAAGGAAG ATCTGCAGTT AGCACTGAAG 
GAGGGGAACA GCATCCTGGA GAGCCTCAGA GAGCCACTGG CTGAGAGCGC GGCCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAGACGGAGG CTGCCTTTGA CGAATTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAAGGTTTC CGGGAGGTCA AAACCACCTT GGATTCCATG 
TCCCAGAAGA TAGCTGCTTT CACGGATGTT GGCAACAGCC TGGCCCACGT GCAGCACCTT 
CTGAAGGACT TGACCGCCTT TGAGGAGAAG TCCAGTGTGG CTGTGGACAA GGCCCGCGCC 
CTGTCACTGG AAGGTCAGCA GCTGATTGAG AACAGGCACT ACGCTGTGGA CTCCATCCAC 
CCCAAGTGTG AAGAGCTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GGATCTTCCT GCTGGCCTCG CAGCCTGTGG ACAAATGCCA GTCCCAGGAT 
GGCGCAGAGG CGGCCCTCCA GGAGATTGAG AAGTTTCTGG AGACCGGTGC AGAAAATAAG 
ATTCAGGAGC TCAATGAGAT TTACAAGGAA TACGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAAAGCACGG AGGAGATGTT CCACCGCAGG 
CAAGCCAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTTACCAA GTCGCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCACTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAAC CCCGGCAGGG CCGCACCAGC TCCACAGGGG AAGAGGAAGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAGCTT CTGGACACCG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCCGCA GAGATGGATA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CATTTTCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATAGACTG CCCAGAGCTG 
GTGGGGAGGT GCTTTCTGGA AAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCTGAGAG CCTGTGGAGG CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG 
TGTCAGAAGA AGCTGGACCA CAAGCTGAGC CTGGACTCCT ACCTGCTGAA ACCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC TCCATCCTGG GCATCCTCAA GGCTGTGAAC 
GACTCCATGC ATCTCATCGC CATCACTGGC TATGATGGAA ACCTTGGCGA CCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGCGTGTGG ACAGACCACA AGAAGGGCCA CACCAAAGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTGT TTCTGCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGATACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACGGCTGTC GGCATCACAG AGAACGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ATATCATTCA GGCGCCAACC 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCTTGCCGGG AGGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCTTTGCCC 
ACGCCATCCA GCACCAGTCC CACAAAAGGG AACACAAGAA ATGTCAAGAA GCTCGAAGAC 
CGGAAGACGG ACCCGCTCAG CCTGGAAGGC TACGTGAGCT CATCGTTACC AAAGCCCCCT 
GAGAAGGGCA AAGGCTGGAG CAAAACATCC CACTCCCTGG AGGCCCCTGA GGAGGACGGA 
GGCTGGTCCA GTGCTGAGGA ACTCATCAAC TCATCTGATG CTGAGGAAGA CGGTGGAGTG 
GGCCCTAAGA AGCTGGTGCC GGGTAAATAC ACAGTGGTGA TGGATGATGA GAAAGGGGGC 
CCTGACACCC TTGCCATGAG GAGTGGAGAT ATGGTGGAGG TGGTGGAAGA AGGCGCTGAG 
GGCCTCTGGT ATGTTCGGGA CCTGACCAGC AGCAAGGAGG GATGGGTGCC AGCCAGCAGC 
CTATCCACCC TCCTGGGCAA ATCCAGTTCA GCCCAGTGTC TGAGCAGCTC AGACTCCAGC 
CCAGGGTCAG CCGTGCTGAG CAGCTCATCC AGCTTCAGCG AGGGCTACCC TGCTCCCTTC 
TCCGAGCTCC AGGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508721.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508784.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OMu40348
Clone ID Related Accession (Same CDS sequence)	XM_006508721.3
Accession Version	XM_006508721.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508721.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAA AGGGTGCGTC CCGGGGGGCC GGGCGGCGGT TCTGGTCACT ATCAAGGAAG 
CGTCGGGGAG CACCAGGGCG CCACAAGCCA GATGAAATCA TGCACCAGGA CATCGTCCCG 
CTCTGTGCCG CTGACATCCA GGAGCAGCTG AAGAAGCGCT TTGCTTACCT GTCTGGTGGG 
CGGGGACAGG ACGGAAGCCC AGTCATCACC TTCCCTGACT ACCCAGCCTT CAGCGAGATC 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TATCTCACCA GCATCCCCAG TTTGCAAGAT 
GCTGGCATTG GATTCATCTT GGTCATAGAC CGAAGGCAGG ACAAATGGAC CTCTGTGAAG 
GCATCCGTCC TGCGGATAGC CGCATCTTTC CCAGCCAACC TGCAGCTTGT CCTTGTTCTT 
CGGCCCACGG GCTTTTTCCA GAGGACTCTC TCGGACATTG CCTTCAAGTT CAACAGAGAT 
GAGTTTAAGA TGAAAGTGCC GGTCATGATG CTAAGCTCAG TGCCAGAACT ACACGGCTAC 
ATTGATAAGT CGCAGCTGAC CGAGGACCTC GGGGGGACCC TGGACTACTG CCACTCCAGG 
TGGCTGTGCC ACCGCACAGC AATTGAAAGC TTCGCCCTGA TGGTGAAGCA GACAGCCCAG 
ATGCTGCAGG CCTTTGGGAC CGAGCTGGCT GAAACAGAGC TGCCGAATGA CGTCCAGTCA 
ACCAGCCTGG TGCTCAGCGC ACACACAGAG AAGAAGGCCA AAGTGAAGGA AGATCTGCAG 
TTAGCACTGA AGGAGGGGAA CAGCATCCTG GAGAGCCTCA GAGAGCCACT GGCTGAGAGC 
GCGGCCCACA GTGTGAACCA GGACCAGCTG GACAATCAGG CCACCGTGCA GAGGCTCCTG 
GCCCAGCTGA ATGAGACGGA GGCTGCCTTT GACGAATTCT GGGCAAAGCA TCAGCAGAAG 
CTGGAACAGT GCCTGCAGCT GCGGCATTTT GAGCAAGGTT TCCGGGAGGT CAAAACCACC 
TTGGATTCCA TGTCCCAGAA GATAGCTGCT TTCACGGATG TTGGCAACAG CCTGGCCCAC 
GTGCAGCACC TTCTGAAGGA CTTGACCGCC TTTGAGGAGA AGTCCAGTGT GGCTGTGGAC 
AAGGCCCGCG CCCTGTCACT GGAAGGTCAG CAGCTGATTG AGAACAGGCA CTACGCTGTG 
GACTCCATCC ACCCCAAGTG TGAAGAGCTC CAGCACCTCT GTGACCACTT CGCCTCTGAG 
GTCACCAGGA GGCGGGGCCT GCTCAGCAAG TCCTTGGAGC TGCATAGCCT CCTGGAGACG 
TCCATGAAGT GGAGCGACGA GGGGATCTTC CTGCTGGCCT CGCAGCCTGT GGACAAATGC 
CAGTCCCAGG ATGGCGCAGA GGCGGCCCTC CAGGAGATTG AGAAGTTTCT GGAGACCGGT 
GCAGAAAATA AGATTCAGGA GCTCAATGAG ATTTACAAGG AATACGAATG CATCCTCAAC 
CAGGACCTTC TGGAACACGT GCAAAAAGTC TTTCAGAAGC AAGAAAGCAC GGAGGAGATG 
TTCCACCGCA GGCAAGCCAG CCTGAAGAAA CTGGCAGCCA AGCAGACACG GCCTGTGCAG 
CCCGTGGCCC CCCGACCAGA GGCGCTTACC AAGTCGCCCT CCCCCTCGCC AGGCTCCTGG 
CGAAGCTCTG AGAACTCCAG CTCTGAGGGC AACGCACTCC GCAGAGGGCC CTACAGGAGA 
GCCAAGAGCG AAATGAGTGA ACCCCGGCAG GGCCGCACCA GCTCCACAGG GGAAGAGGAA 
GAGAGCCTGG CCATCCTGCG CAGACACGTG ATGAATGAGC TTCTGGACAC CGAGCGGGCC 
TACGTGGAGG AGCTGCTCTG TGTCTTAGAG GGCTATGCCG CAGAGATGGA TAACCCCTTG 
ATGGCACACC TCATCTCAAC AGGCCTGCAA AACAAGAAGA ACATTCTGTT TGGAAACATG 
GAGGAAATCT ATCATTTTCA CAACAGAATA TTCCTGCGGG AGCTGGAGAG CTGCATAGAC 
TGCCCAGAGC TGGTGGGGAG GTGCTTTCTG GAAAGGATGG AGGAGTTCCA GATCTATGAG 
AAGTACTGTC AGAACAAGCC ACGCTCTGAG AGCCTGTGGA GGCAGTGCTC TGACTGCCCC 
TTCTTCCAGG AGTGTCAGAA GAAGCTGGAC CACAAGCTGA GCCTGGACTC CTACCTGCTG 
AAACCTGTGC AGAGGATAAC CAAGTACCAG CTGCTGCTCA AGGAAATGCT GAAGTACAGC 
AAGCACTGTG AAGGGGCAGA GGACCTGCAG GAGGCGCTGA GCTCCATCCT GGGCATCCTC 
AAGGCTGTGA ACGACTCCAT GCATCTCATC GCCATCACTG GCTATGATGG AAACCTTGGC 
GACCTGGGGA AGCTGCTGAT GCAAGGTTCC TTCAGCGTGT GGACAGACCA CAAGAAGGGC 
CACACCAAAG TGAAGGAGCT AGCCAGGTTC AAGCCCATGC AGCGGCACCT GTTTCTGCAC 
GAGAAGGCCG TCCTCTTCTG CAAGAAGAGG GAGGAGAATG GGGAGGGATA CGAGAAGGCT 
CCTTCCTACA GCTACAAGCA GTCCTTGAAT ATGACGGCTG TCGGCATCAC AGAGAACGTG 
AAGGGTGACA CCAAGAAGTT CGAGATCTGG TACAACGCAA GGGAGGAGGT TTATATCATT 
CAGGCGCCAA CCCCCGAGAT TAAAGCAGCG TGGGTGAATG AGATTCGGAA GGTGCTGACC 
AGCCAGCTGC AGGCTTGCCG GGAGGCCAGC CAGCACCGAG CCCTGGAGCA GTCCCACAGC 
CTTCCTTTGC CCACGCCATC CAGCACCAGT CCCACAAAAG GGAACACAAG AAATGTCAAG 
AAGCTCGAAG ACCGGAAGAC GGACCCGCTC AGCCTGGAAG GCTACGTGAG CTCATCGTTA 
CCAAAGCCCC CTGAGAAGGG CAAAGGCTGG AGCAAAACAT CCCACTCCCT GGAGGCCCCT 
GAGGAGGACG GAGGCTGGTC CAGTGCTGAG GAACTCATCA ACTCATCTGA TGCTGAGGAA 
GACGGTGGAG TGGGCCCTAA GAAGCTGGTG CCGGGTAAAT ACACAGTGGT GATGGATGAT 
GAGAAAGGGG GCCCTGACAC CCTTGCCATG AGGAGTGGAG ATATGGTGGA GGTGGTGGAA 
GAAGGCGCTG AGGGCCTCTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508784.1
CDS	282..3485
Translation	MAEKGASRGAGRRFWSLSRKRRGAPGRHKPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLW

Target ORF information:

RefSeq Version	XM_006508721.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508721.3

ORF Insert Sequence:

ATGGCAGAAA AGGGTGCGTC CCGGGGGGCC GGGCGGCGGT TCTGGTCACT ATCAAGGAAG 
CGTCGGGGAG CACCAGGGCG CCACAAGCCA GATGAAATCA TGCACCAGGA CATCGTCCCG 
CTCTGTGCCG CTGACATCCA GGAGCAGCTG AAGAAGCGCT TTGCTTACCT GTCTGGTGGG 
CGGGGACAGG ACGGAAGCCC AGTCATCACC TTCCCTGACT ACCCAGCCTT CAGCGAGATC 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TATCTCACCA GCATCCCCAG TTTGCAAGAT 
GCTGGCATTG GATTCATCTT GGTCATAGAC CGAAGGCAGG ACAAATGGAC CTCTGTGAAG 
GCATCCGTCC TGCGGATAGC CGCATCTTTC CCAGCCAACC TGCAGCTTGT CCTTGTTCTT 
CGGCCCACGG GCTTTTTCCA GAGGACTCTC TCGGACATTG CCTTCAAGTT CAACAGAGAT 
GAGTTTAAGA TGAAAGTGCC GGTCATGATG CTAAGCTCAG TGCCAGAACT ACACGGCTAC 
ATTGATAAGT CGCAGCTGAC CGAGGACCTC GGGGGGACCC TGGACTACTG CCACTCCAGG 
TGGCTGTGCC ACCGCACAGC AATTGAAAGC TTCGCCCTGA TGGTGAAGCA GACAGCCCAG 
ATGCTGCAGG CCTTTGGGAC CGAGCTGGCT GAAACAGAGC TGCCGAATGA CGTCCAGTCA 
ACCAGCCTGG TGCTCAGCGC ACACACAGAG AAGAAGGCCA AAGTGAAGGA AGATCTGCAG 
TTAGCACTGA AGGAGGGGAA CAGCATCCTG GAGAGCCTCA GAGAGCCACT GGCTGAGAGC 
GCGGCCCACA GTGTGAACCA GGACCAGCTG GACAATCAGG CCACCGTGCA GAGGCTCCTG 
GCCCAGCTGA ATGAGACGGA GGCTGCCTTT GACGAATTCT GGGCAAAGCA TCAGCAGAAG 
CTGGAACAGT GCCTGCAGCT GCGGCATTTT GAGCAAGGTT TCCGGGAGGT CAAAACCACC 
TTGGATTCCA TGTCCCAGAA GATAGCTGCT TTCACGGATG TTGGCAACAG CCTGGCCCAC 
GTGCAGCACC TTCTGAAGGA CTTGACCGCC TTTGAGGAGA AGTCCAGTGT GGCTGTGGAC 
AAGGCCCGCG CCCTGTCACT GGAAGGTCAG CAGCTGATTG AGAACAGGCA CTACGCTGTG 
GACTCCATCC ACCCCAAGTG TGAAGAGCTC CAGCACCTCT GTGACCACTT CGCCTCTGAG 
GTCACCAGGA GGCGGGGCCT GCTCAGCAAG TCCTTGGAGC TGCATAGCCT CCTGGAGACG 
TCCATGAAGT GGAGCGACGA GGGGATCTTC CTGCTGGCCT CGCAGCCTGT GGACAAATGC 
CAGTCCCAGG ATGGCGCAGA GGCGGCCCTC CAGGAGATTG AGAAGTTTCT GGAGACCGGT 
GCAGAAAATA AGATTCAGGA GCTCAATGAG ATTTACAAGG AATACGAATG CATCCTCAAC 
CAGGACCTTC TGGAACACGT GCAAAAAGTC TTTCAGAAGC AAGAAAGCAC GGAGGAGATG 
TTCCACCGCA GGCAAGCCAG CCTGAAGAAA CTGGCAGCCA AGCAGACACG GCCTGTGCAG 
CCCGTGGCCC CCCGACCAGA GGCGCTTACC AAGTCGCCCT CCCCCTCGCC AGGCTCCTGG 
CGAAGCTCTG AGAACTCCAG CTCTGAGGGC AACGCACTCC GCAGAGGGCC CTACAGGAGA 
GCCAAGAGCG AAATGAGTGA ACCCCGGCAG GGCCGCACCA GCTCCACAGG GGAAGAGGAA 
GAGAGCCTGG CCATCCTGCG CAGACACGTG ATGAATGAGC TTCTGGACAC CGAGCGGGCC 
TACGTGGAGG AGCTGCTCTG TGTCTTAGAG GGCTATGCCG CAGAGATGGA TAACCCCTTG 
ATGGCACACC TCATCTCAAC AGGCCTGCAA AACAAGAAGA ACATTCTGTT TGGAAACATG 
GAGGAAATCT ATCATTTTCA CAACAGAATA TTCCTGCGGG AGCTGGAGAG CTGCATAGAC 
TGCCCAGAGC TGGTGGGGAG GTGCTTTCTG GAAAGGATGG AGGAGTTCCA GATCTATGAG 
AAGTACTGTC AGAACAAGCC ACGCTCTGAG AGCCTGTGGA GGCAGTGCTC TGACTGCCCC 
TTCTTCCAGG AGTGTCAGAA GAAGCTGGAC CACAAGCTGA GCCTGGACTC CTACCTGCTG 
AAACCTGTGC AGAGGATAAC CAAGTACCAG CTGCTGCTCA AGGAAATGCT GAAGTACAGC 
AAGCACTGTG AAGGGGCAGA GGACCTGCAG GAGGCGCTGA GCTCCATCCT GGGCATCCTC 
AAGGCTGTGA ACGACTCCAT GCATCTCATC GCCATCACTG GCTATGATGG AAACCTTGGC 
GACCTGGGGA AGCTGCTGAT GCAAGGTTCC TTCAGCGTGT GGACAGACCA CAAGAAGGGC 
CACACCAAAG TGAAGGAGCT AGCCAGGTTC AAGCCCATGC AGCGGCACCT GTTTCTGCAC 
GAGAAGGCCG TCCTCTTCTG CAAGAAGAGG GAGGAGAATG GGGAGGGATA CGAGAAGGCT 
CCTTCCTACA GCTACAAGCA GTCCTTGAAT ATGACGGCTG TCGGCATCAC AGAGAACGTG 
AAGGGTGACA CCAAGAAGTT CGAGATCTGG TACAACGCAA GGGAGGAGGT TTATATCATT 
CAGGCGCCAA CCCCCGAGAT TAAAGCAGCG TGGGTGAATG AGATTCGGAA GGTGCTGACC 
AGCCAGCTGC AGGCTTGCCG GGAGGCCAGC CAGCACCGAG CCCTGGAGCA GTCCCACAGC 
CTTCCTTTGC CCACGCCATC CAGCACCAGT CCCACAAAAG GGAACACAAG AAATGTCAAG 
AAGCTCGAAG ACCGGAAGAC GGACCCGCTC AGCCTGGAAG GCTACGTGAG CTCATCGTTA 
CCAAAGCCCC CTGAGAAGGG CAAAGGCTGG AGCAAAACAT CCCACTCCCT GGAGGCCCCT 
GAGGAGGACG GAGGCTGGTC CAGTGCTGAG GAACTCATCA ACTCATCTGA TGCTGAGGAA 
GACGGTGGAG TGGGCCCTAA GAAGCTGGTG CCGGGTAAAT ACACAGTGGT GATGGATGAT 
GAGAAAGGGG GCCCTGACAC CCTTGCCATG AGGAGTGGAG ATATGGTGGA GGTGGTGGAA 
GAAGGCGCTG AGGGCCTCTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508722.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508785.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OMu40349
Clone ID Related Accession (Same CDS sequence)	XM_006508722.2
Accession Version	XM_006508722.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006508722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTGC CGGTCATGAT GCTAAGCTCA GTGCCAGAAC TACACGGCTA CATTGATAAG 
TCGCAGCTGA CCGAGGACCT CGGGGGGACC CTGGACTACT GCCACTCCAG GTGGCTGTGC 
CACCGCACAG CAATTGAAAG CTTCGCCCTG ATGGTGAAGC AGACAGCCCA GATGCTGCAG 
GCCTTTGGGA CCGAGCTGGC TGAAACAGAG CTGCCGAATG ACGTCCAGTC AACCAGCCTG 
GTGCTCAGCG CACACACAGA GAAGAAGGCC AAAGTGAAGG AAGATCTGCA GTTAGCACTG 
AAGGAGGGGA ACAGCATCCT GGAGAGCCTC AGAGAGCCAC TGGCTGAGAG CGCGGCCCAC 
AGTGTGAACC AGGACCAGCT GGACAATCAG GCCACCGTGC AGAGGCTCCT GGCCCAGCTG 
AATGAGACGG AGGCTGCCTT TGACGAATTC TGGGCAAAGC ATCAGCAGAA GCTGGAACAG 
TGCCTGCAGC TGCGGCATTT TGAGCAAGGT TTCCGGGAGG TCAAAACCAC CTTGGATTCC 
ATGTCCCAGA AGATAGCTGC TTTCACGGAT GTTGGCAACA GCCTGGCCCA CGTGCAGCAC 
CTTCTGAAGG ACTTGACCGC CTTTGAGGAG AAGTCCAGTG TGGCTGTGGA CAAGGCCCGC 
GCCCTGTCAC TGGAAGGTCA GCAGCTGATT GAGAACAGGC ACTACGCTGT GGACTCCATC 
CACCCCAAGT GTGAAGAGCT CCAGCACCTC TGTGACCACT TCGCCTCTGA GGTCACCAGG 
AGGCGGGGCC TGCTCAGCAA GTCCTTGGAG CTGCATAGCC TCCTGGAGAC GTCCATGAAG 
TGGAGCGACG AGGGGATCTT CCTGCTGGCC TCGCAGCCTG TGGACAAATG CCAGTCCCAG 
GATGGCGCAG AGGCGGCCCT CCAGGAGATT GAGAAGTTTC TGGAGACCGG TGCAGAAAAT 
AAGATTCAGG AGCTCAATGA GATTTACAAG GAATACGAAT GCATCCTCAA CCAGGACCTT 
CTGGAACACG TGCAAAAAGT CTTTCAGAAG CAAGAAAGCA CGGAGGAGAT GTTCCACCGC 
AGGCAAGCCA GCCTGAAGAA ACTGGCAGCC AAGCAGACAC GGCCTGTGCA GCCCGTGGCC 
CCCCGACCAG AGGCGCTTAC CAAGTCGCCC TCCCCCTCGC CAGGCTCCTG GCGAAGCTCT 
GAGAACTCCA GCTCTGAGGG CAACGCACTC CGCAGAGGGC CCTACAGGAG AGCCAAGAGC 
GAAATGAGTG AACCCCGGCA GGGCCGCACC AGCTCCACAG GGGAAGAGGA AGAGAGCCTG 
GCCATCCTGC GCAGACACGT GATGAATGAG CTTCTGGACA CCGAGCGGGC CTACGTGGAG 
GAGCTGCTCT GTGTCTTAGA GGGCTATGCC GCAGAGATGG ATAACCCCTT GATGGCACAC 
CTCATCTCAA CAGGCCTGCA AAACAAGAAG AACATTCTGT TTGGAAACAT GGAGGAAATC 
TATCATTTTC ACAACAGAAT ATTCCTGCGG GAGCTGGAGA GCTGCATAGA CTGCCCAGAG 
CTGGTGGGGA GGTGCTTTCT GGAAAGGATG GAGGAGTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CACGCTCTGA GAGCCTGTGG AGGCAGTGCT CTGACTGCCC CTTCTTCCAG 
GAGTGTCAGA AGAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAACCTGTG 
CAGAGGATAA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAGTACAG CAAGCACTGT 
GAAGGGGCAG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT CAAGGCTGTG 
AACGACTCCA TGCATCTCAT CGCCATCACT GGCTATGATG GAAACCTTGG CGACCTGGGG 
AAGCTGCTGA TGCAAGGTTC CTTCAGCGTG TGGACAGACC ACAAGAAGGG CCACACCAAA 
GTGAAGGAGC TAGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTTCTGCA CGAGAAGGCC 
GTCCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGAT ACGAGAAGGC TCCTTCCTAC 
AGCTACAAGC AGTCCTTGAA TATGACGGCT GTCGGCATCA CAGAGAACGT GAAGGGTGAC 
ACCAAGAAGT TCGAGATCTG GTACAACGCA AGGGAGGAGG TTTATATCAT TCAGGCGCCA 
ACCCCCGAGA TTAAAGCAGC GTGGGTGAAT GAGATTCGGA AGGTGCTGAC CAGCCAGCTG 
CAGGCTTGCC GGGAGGCCAG CCAGCACCGA GCCCTGGAGC AGTCCCACAG CCTTCCTTTG 
CCCACGCCAT CCAGCACCAG TCCCACAAAA GGGAACACAA GAAATGTCAA GAAGCTCGAA 
GACCGGAAGA CGGACCCGCT CAGCCTGGAA GGCTACGTGA GCTCATCGTT ACCAAAGCCC 
CCTGAGAAGG GCAAAGACGA TGCGGTCCCT AGTTCTACCT CTGAAAGCTC TGCGCTTTCC 
AGAAAGCGCT TCACGCTGCA GGGCTTGGCT AACCTCAAAG GTCAGAAAGC CTCTCCGACC 
AGTCCTGACA AAAAAGCTAA GCGGCACGAA GTAAAGAGCG ACCCGACTCC CTTTGGTTTG 
CGAGGCTGGA GCAAAACATC CCACTCCCTG GAGGCCCCTG AGGAGGACGG AGGCTGGTCC 
AGTGCTGAGG AACTCATCAA CTCATCTGAT GCTGAGGAAG ACGGTGGAGT GGGCCCTAAG 
AAGCTGGTGC CGGGTAAATA CACAGTGGTG ATGGATGATG AGAAAGGGGG CCCTGACACC 
CTTGCCATGA GGAGTGGAGA TATGGTGGAG GTGGTGGAAG AAGGCGCTGA GGGCCTCTGG 
TATGTTCGGG ACCTGACCAG CAGCAAGGAG GGATGGGTGC CAGCCAGCAG CCTATCCACC 
CTCCTGGGCA AATCCAGTTC AGCCCAGTGT CTGAGCAGCT CAGACTCCAG CCCAGGGTCA 
GCCGTGCTGA GCAGCTCATC CAGCTTCAGC GAGGGCTACC CTGCTCCCTT CTCCGAGCTC 
CAGGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508785.1
CDS	583..3651
Translation	MKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSSFSEGYPAPFSELQG

Target ORF information:

RefSeq Version	XM_006508722.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508722.2

ORF Insert Sequence:

ATGAAAGTGC CGGTCATGAT GCTAAGCTCA GTGCCAGAAC TACACGGCTA CATTGATAAG 
TCGCAGCTGA CCGAGGACCT CGGGGGGACC CTGGACTACT GCCACTCCAG GTGGCTGTGC 
CACCGCACAG CAATTGAAAG CTTCGCCCTG ATGGTGAAGC AGACAGCCCA GATGCTGCAG 
GCCTTTGGGA CCGAGCTGGC TGAAACAGAG CTGCCGAATG ACGTCCAGTC AACCAGCCTG 
GTGCTCAGCG CACACACAGA GAAGAAGGCC AAAGTGAAGG AAGATCTGCA GTTAGCACTG 
AAGGAGGGGA ACAGCATCCT GGAGAGCCTC AGAGAGCCAC TGGCTGAGAG CGCGGCCCAC 
AGTGTGAACC AGGACCAGCT GGACAATCAG GCCACCGTGC AGAGGCTCCT GGCCCAGCTG 
AATGAGACGG AGGCTGCCTT TGACGAATTC TGGGCAAAGC ATCAGCAGAA GCTGGAACAG 
TGCCTGCAGC TGCGGCATTT TGAGCAAGGT TTCCGGGAGG TCAAAACCAC CTTGGATTCC 
ATGTCCCAGA AGATAGCTGC TTTCACGGAT GTTGGCAACA GCCTGGCCCA CGTGCAGCAC 
CTTCTGAAGG ACTTGACCGC CTTTGAGGAG AAGTCCAGTG TGGCTGTGGA CAAGGCCCGC 
GCCCTGTCAC TGGAAGGTCA GCAGCTGATT GAGAACAGGC ACTACGCTGT GGACTCCATC 
CACCCCAAGT GTGAAGAGCT CCAGCACCTC TGTGACCACT TCGCCTCTGA GGTCACCAGG 
AGGCGGGGCC TGCTCAGCAA GTCCTTGGAG CTGCATAGCC TCCTGGAGAC GTCCATGAAG 
TGGAGCGACG AGGGGATCTT CCTGCTGGCC TCGCAGCCTG TGGACAAATG CCAGTCCCAG 
GATGGCGCAG AGGCGGCCCT CCAGGAGATT GAGAAGTTTC TGGAGACCGG TGCAGAAAAT 
AAGATTCAGG AGCTCAATGA GATTTACAAG GAATACGAAT GCATCCTCAA CCAGGACCTT 
CTGGAACACG TGCAAAAAGT CTTTCAGAAG CAAGAAAGCA CGGAGGAGAT GTTCCACCGC 
AGGCAAGCCA GCCTGAAGAA ACTGGCAGCC AAGCAGACAC GGCCTGTGCA GCCCGTGGCC 
CCCCGACCAG AGGCGCTTAC CAAGTCGCCC TCCCCCTCGC CAGGCTCCTG GCGAAGCTCT 
GAGAACTCCA GCTCTGAGGG CAACGCACTC CGCAGAGGGC CCTACAGGAG AGCCAAGAGC 
GAAATGAGTG AACCCCGGCA GGGCCGCACC AGCTCCACAG GGGAAGAGGA AGAGAGCCTG 
GCCATCCTGC GCAGACACGT GATGAATGAG CTTCTGGACA CCGAGCGGGC CTACGTGGAG 
GAGCTGCTCT GTGTCTTAGA GGGCTATGCC GCAGAGATGG ATAACCCCTT GATGGCACAC 
CTCATCTCAA CAGGCCTGCA AAACAAGAAG AACATTCTGT TTGGAAACAT GGAGGAAATC 
TATCATTTTC ACAACAGAAT ATTCCTGCGG GAGCTGGAGA GCTGCATAGA CTGCCCAGAG 
CTGGTGGGGA GGTGCTTTCT GGAAAGGATG GAGGAGTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CACGCTCTGA GAGCCTGTGG AGGCAGTGCT CTGACTGCCC CTTCTTCCAG 
GAGTGTCAGA AGAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAACCTGTG 
CAGAGGATAA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAGTACAG CAAGCACTGT 
GAAGGGGCAG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT CAAGGCTGTG 
AACGACTCCA TGCATCTCAT CGCCATCACT GGCTATGATG GAAACCTTGG CGACCTGGGG 
AAGCTGCTGA TGCAAGGTTC CTTCAGCGTG TGGACAGACC ACAAGAAGGG CCACACCAAA 
GTGAAGGAGC TAGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTTCTGCA CGAGAAGGCC 
GTCCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGAT ACGAGAAGGC TCCTTCCTAC 
AGCTACAAGC AGTCCTTGAA TATGACGGCT GTCGGCATCA CAGAGAACGT GAAGGGTGAC 
ACCAAGAAGT TCGAGATCTG GTACAACGCA AGGGAGGAGG TTTATATCAT TCAGGCGCCA 
ACCCCCGAGA TTAAAGCAGC GTGGGTGAAT GAGATTCGGA AGGTGCTGAC CAGCCAGCTG 
CAGGCTTGCC GGGAGGCCAG CCAGCACCGA GCCCTGGAGC AGTCCCACAG CCTTCCTTTG 
CCCACGCCAT CCAGCACCAG TCCCACAAAA GGGAACACAA GAAATGTCAA GAAGCTCGAA 
GACCGGAAGA CGGACCCGCT CAGCCTGGAA GGCTACGTGA GCTCATCGTT ACCAAAGCCC 
CCTGAGAAGG GCAAAGACGA TGCGGTCCCT AGTTCTACCT CTGAAAGCTC TGCGCTTTCC 
AGAAAGCGCT TCACGCTGCA GGGCTTGGCT AACCTCAAAG GTCAGAAAGC CTCTCCGACC 
AGTCCTGACA AAAAAGCTAA GCGGCACGAA GTAAAGAGCG ACCCGACTCC CTTTGGTTTG 
CGAGGCTGGA GCAAAACATC CCACTCCCTG GAGGCCCCTG AGGAGGACGG AGGCTGGTCC 
AGTGCTGAGG AACTCATCAA CTCATCTGAT GCTGAGGAAG ACGGTGGAGT GGGCCCTAAG 
AAGCTGGTGC CGGGTAAATA CACAGTGGTG ATGGATGATG AGAAAGGGGG CCCTGACACC 
CTTGCCATGA GGAGTGGAGA TATGGTGGAG GTGGTGGAAG AAGGCGCTGA GGGCCTCTGG 
TATGTTCGGG ACCTGACCAG CAGCAAGGAG GGATGGGTGC CAGCCAGCAG CCTATCCACC 
CTCCTGGGCA AATCCAGTTC AGCCCAGTGT CTGAGCAGCT CAGACTCCAG CCCAGGGTCA 
GCCGTGCTGA GCAGCTCATC CAGCTTCAGC GAGGGCTACC CTGCTCCCTT CTCCGAGCTC 
CAGGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006508723.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508786.1 Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X22, mRNA.

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CloneID	OMu40350
Clone ID Related Accession (Same CDS sequence)	XM_006508723.3
Accession Version	XM_006508723.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange factor DBS isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508723.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGGTC ACTGATGCCT 
TCAGTCCCAC AAAAGGGAAC ACAAGAAATG TCAAGAAGCT CGAAGACCGG AAGACGGACC 
CGCTCAGCCT GGAAGGCTAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508786.1
CDS	303..3326
Translation	MSDCWCFIFCKEHVRSNPLSPQHDGASREEADHQVDVSDGIRLVPDKAEATAATASDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTRSLMPSVPQKGTQEMSRSSKTGRRTRSAWKAT

Target ORF information:

RefSeq Version	XM_006508723.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508723.3

ORF Insert Sequence:

ATGTCCGACT GCTGGTGCTT CATCTTCTGC AAGGAACACG TTAGAAGCAA TCCTCTGTCA 
CCACAGCATG ATGGGGCCTC AAGGGAGGAG GCTGATCACC AGGTGGACGT GTCTGATGGC 
ATCAGGCTGG TTCCAGACAA AGCAGAGGCC ACGGCTGCTA CAGCCTCAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCCGCT GACATCCAGG AGCAGCTGAA GAAGCGCTTT 
GCTTACCTGT CTGGTGGGCG GGGACAGGAC GGAAGCCCAG TCATCACCTT CCCTGACTAC 
CCAGCCTTCA GCGAGATCCC GGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGTT TGCAAGATGC TGGCATTGGA TTCATCTTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CTGTGAAGGC ATCCGTCCTG CGGATAGCCG CATCTTTCCC AGCCAACCTG 
CAGCTTGTCC TTGTTCTTCG GCCCACGGGC TTTTTCCAGA GGACTCTCTC GGACATTGCC 
TTCAAGTTCA ACAGAGATGA GTTTAAGATG AAAGTGCCGG TCATGATGCT AAGCTCAGTG 
CCAGAACTAC ACGGCTACAT TGATAAGTCG CAGCTGACCG AGGACCTCGG GGGGACCCTG 
GACTACTGCC ACTCCAGGTG GCTGTGCCAC CGCACAGCAA TTGAAAGCTT CGCCCTGATG 
GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC TTTGGGACCG AGCTGGCTGA AACAGAGCTG 
CCGAATGACG TCCAGTCAAC CAGCCTGGTG CTCAGCGCAC ACACAGAGAA GAAGGCCAAA 
GTGAAGGAAG ATCTGCAGTT AGCACTGAAG GAGGGGAACA GCATCCTGGA GAGCCTCAGA 
GAGCCACTGG CTGAGAGCGC GGCCCACAGT GTGAACCAGG ACCAGCTGGA CAATCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAGACGGAGG CTGCCTTTGA CGAATTCTGG 
GCAAAGCATC AGCAGAAGCT GGAACAGTGC CTGCAGCTGC GGCATTTTGA GCAAGGTTTC 
CGGGAGGTCA AAACCACCTT GGATTCCATG TCCCAGAAGA TAGCTGCTTT CACGGATGTT 
GGCAACAGCC TGGCCCACGT GCAGCACCTT CTGAAGGACT TGACCGCCTT TGAGGAGAAG 
TCCAGTGTGG CTGTGGACAA GGCCCGCGCC CTGTCACTGG AAGGTCAGCA GCTGATTGAG 
AACAGGCACT ACGCTGTGGA CTCCATCCAC CCCAAGTGTG AAGAGCTCCA GCACCTCTGT 
GACCACTTCG CCTCTGAGGT CACCAGGAGG CGGGGCCTGC TCAGCAAGTC CTTGGAGCTG 
CATAGCCTCC TGGAGACGTC CATGAAGTGG AGCGACGAGG GGATCTTCCT GCTGGCCTCG 
CAGCCTGTGG ACAAATGCCA GTCCCAGGAT GGCGCAGAGG CGGCCCTCCA GGAGATTGAG 
AAGTTTCTGG AGACCGGTGC AGAAAATAAG ATTCAGGAGC TCAATGAGAT TTACAAGGAA 
TACGAATGCA TCCTCAACCA GGACCTTCTG GAACACGTGC AAAAAGTCTT TCAGAAGCAA 
GAAAGCACGG AGGAGATGTT CCACCGCAGG CAAGCCAGCC TGAAGAAACT GGCAGCCAAG 
CAGACACGGC CTGTGCAGCC CGTGGCCCCC CGACCAGAGG CGCTTACCAA GTCGCCCTCC 
CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG AACTCCAGCT CTGAGGGCAA CGCACTCCGC 
AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA ATGAGTGAAC CCCGGCAGGG CCGCACCAGC 
TCCACAGGGG AAGAGGAAGA GAGCCTGGCC ATCCTGCGCA GACACGTGAT GAATGAGCTT 
CTGGACACCG AGCGGGCCTA CGTGGAGGAG CTGCTCTGTG TCTTAGAGGG CTATGCCGCA 
GAGATGGATA ACCCCTTGAT GGCACACCTC ATCTCAACAG GCCTGCAAAA CAAGAAGAAC 
ATTCTGTTTG GAAACATGGA GGAAATCTAT CATTTTCACA ACAGAATATT CCTGCGGGAG 
CTGGAGAGCT GCATAGACTG CCCAGAGCTG GTGGGGAGGT GCTTTCTGGA AAGGATGGAG 
GAGTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCAC GCTCTGAGAG CCTGTGGAGG 
CAGTGCTCTG ACTGCCCCTT CTTCCAGGAG TGTCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA ACCTGTGCAG AGGATAACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AGTACAGCAA GCACTGTGAA GGGGCAGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCTGTGAAC GACTCCATGC ATCTCATCGC CATCACTGGC 
TATGATGGAA ACCTTGGCGA CCTGGGGAAG CTGCTGATGC AAGGTTCCTT CAGCGTGTGG 
ACAGACCACA AGAAGGGCCA CACCAAAGTG AAGGAGCTAG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TTCTGCACGA GAAGGCCGTC CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGATACG AGAAGGCTCC TTCCTACAGC TACAAGCAGT CCTTGAATAT GACGGCTGTC 
GGCATCACAG AGAACGTGAA GGGTGACACC AAGAAGTTCG AGATCTGGTA CAACGCAAGG 
GAGGAGGTTT ATATCATTCA GGCGCCAACC CCCGAGATTA AAGCAGCGTG GGTGAATGAG 
ATTCGGAAGG TGCTGACCAG CCAGCTGCAG GCTTGCCGGG AGGCCAGCCA GCACCGAGCC 
CTGGAGCAGT CCCACAGCCT TCCTTTGCCC ACGCCATCCA GCACCAGGTC ACTGATGCCT 
TCAGTCCCAC AAAAGGGAAC ACAAGAAATG TCAAGAAGCT CGAAGACCGG AAGACGGACC 
CGCTCAGCCT GGAAGGCTAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ptbp2 represses adult-specific splicing to regulate the generation of neuronal precursors in the embryonic brain.
Genes & development26(14)1626-42(2012 Jul)
Licatalosi DD,Yano M,Fak JJ,Mele A,Grabinski SE,Zhang C,Darnell RB

A mutation in the Srrm4 gene causes alternative splicing defects and deafness in the Bronx waltzer mouse.
PLoS genetics8(10)e1002966(2012)
Nakano Y,Jahan I,Bonde G,Sun X,Hildebrand MS,Engelhardt JF,Smith RJ,Cornell RA,Fritzsch B,B?nfi B

Release of autoinhibition of ASEF by APC leads to CDC42 activation and tumor suppression.
Nature structural & molecular biology14(9)814-23(2007 Sep)
Mitin N,Betts L,Yohe ME,Der CJ,Sondek J,Rossman KL

Role of the guanine nucleotide exchange factor Ost in negative regulation of receptor endocytosis by the small GTPase Rac1.
The Journal of biological chemistry282(32)23296-305(2007 Aug)
Ieguchi K,Ueda S,Kataoka T,Satoh T

The Dbs PH domain contributes independently to membrane targeting and regulation of guanine nucleotide-exchange activity.
The Biochemical journal400(3)563-72(2006 Dec)
Baumeister MA,Rossman KL,Sondek J,Lemmon MA

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Mcf2l cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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