MCF2L gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015465841.1	XP_015321327.1	guanine nucleotide exchange factor DBS isoform X2
XM_010822299.2	XP_010820601.1	guanine nucleotide exchange factor DBS isoform X6
XM_010810999.2	XP_010809301.1	guanine nucleotide exchange factor DBS isoform X6
XM_010810997.3	XP_010809299.2	guanine nucleotide exchange factor DBS isoform X4
XM_025000180.1	XP_024855948.1	guanine nucleotide exchange factor DBS isoform X6
XM_010810998.2	XP_010809300.1	guanine nucleotide exchange factor DBS isoform X5
XM_010810996.2	XP_010809298.1	guanine nucleotide exchange factor DBS isoform X3
XM_015465840.1	XP_015321326.1	guanine nucleotide exchange factor DBS isoform X1
XM_010822296.2	XP_010820598.1	guanine nucleotide exchange factor DBS isoform X3
XM_025000179.1	XP_024855947.1	guanine nucleotide exchange factor DBS isoform X2
XM_015473941.1	XP_015329427.1	guanine nucleotide exchange factor DBS isoform X2
XM_010810996.3	XP_010809298.2	guanine nucleotide exchange factor DBS isoform X3
XM_010822297.2	XP_010820599.1	guanine nucleotide exchange factor DBS isoform X4
XM_010822298.2	XP_010820600.1	guanine nucleotide exchange factor DBS isoform X5
XM_015473940.1	XP_015329426.1	guanine nucleotide exchange factor DBS isoform X1
XM_010810997.2	XP_010809299.1	guanine nucleotide exchange factor DBS isoform X4
XM_025000178.1	XP_024855946.1	guanine nucleotide exchange factor DBS isoform X1
XM_010810999.3	XP_010809301.2	guanine nucleotide exchange factor DBS isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa221355	XM_025000178.1 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa221356	XM_025000179.1 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa221357	XM_010810996.3 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa221358	XM_010810997.3 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa221359	XM_010810999.3 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa221360	XM_025000180.1 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa92205	XM_015473940.1 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92205	XM_015465840.1 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92206	XM_015465841.1 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92206	XM_015473941.1 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa46308	XM_010822296.2 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa46308	XM_010810996.2 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa46309	XM_010822297.2 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa46309	XM_010810997.2 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa46310	XM_010822298.2 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa46310	XM_010810998.2 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa46311	XM_010810999.2 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa46311	XM_010822299.2 Latest version!	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

MCF2L ( XM_025000178.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855946.1 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa221355
Clone ID Related Accession (Same CDS sequence)	XM_025000178.1
Accession Version	XM_025000178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCC CAGCAGCTGA TGCAAAAAGG GGAAACGTTT GGACGCAAGG TCACACGCTC 
AAAAAAACGA AAGGTTCTTC CGGAAGGAAT TCCAGAGGAG CGCAGTCGAC CGGCGGGTGC 
ACTGAAACCC TCAAGGGGGT GTCTTTGGGA TGTTTCCTTT CTTTTGGGAA ATCGATGCAC 
CCGAAATTGT TGAATACGGC ACTGGCTGTG GATGAGATCA TGCACCACGA CATCAGCCCG 
CTCTGTGCCG CCGACATCCG GGACCAGCTG CAGAAGCGCT TCGCTTACCT GTCAGGCGGG 
CGGGGCCAGG ACGGCAGCCC GGTCATCACC TTTCCCGACT ACCCGGCCTT CGGAGACGTC 
CCAGACCAGG ACTTCCAGAA TGTCATGACC TACCTCACCA GCATCCCCAG CCTGCAGGAC 
GCTGGCATCG GGTTCATCCT GGTCATAGAC CGCAGGCAGG ACAGATGGAC CTCGGTCAAG 
GCGTCCATCC TGAGAATAGC GGCATCCTTC CCCGCAAACC TGCAGCTCGT CCTCGTCCTG 
CGGCCAACCG GGTTCTTCCA GAGGACACTC TCCGACCTCG CCTTCAGGTT CAACAGAGAT 
GACTTTAAGA TGAAGGTGCC GGTCATAATG CTGAGCTCAG TCCCAGAACT ACACGGCTAC 
ATCGACAAGT CCCAGCTGAC GGAGGACCTG GGTGGGACGC TGGCCTACTG CCACTCCAGG 
TGGCTCCGCC ATCGCACAGC CATCGAGAGC TTCGCCCTCC TGGTCAAGCA GACGGCGCAG 
ATGCTGCAGA CCTTTGGGAC CGAGCTGGCC GAGACGGAGC TGCCCAATGA CGTGCAGTCC 
ACGAGCTCCG TGCTCCTCGC CCACACGGAG AAGAAGGACA GAGCCAAGGA GGATATGAGG 
CTGGCGCTTG TCGAGGGGCG GCGCGTCCTG GAGAGCATCC ACGAGCCACT GGCCCGGAGC 
CCAGATCAGA GCCCCAACCA GGACCAACTG GACAGTCAGA GCACCGTGCA CAGGCTCCTG 
GCCCAGCTGA ACGAGACCGA GGCCGCCTTT GACGAGTTCT GGGCAAAACA TCAGCAAAAA 
CTGGAGCAGT GCCTGCAGCT CCGGCATTTC GAGCAGGACT TCCGAGAGGT CAAATCGGCC 
CTGGACGTGC TGGCCCAGAA GGTCACGACT TTCACCGACG TGGGCAACAG CCTGGCACAC 
GTGCAGCACC TCCTGAAGGA CCTGACCAGC TTCGAGGAGA AGTCCAGTGC CGCCGTGCAG 
CGGGCCCGCA CGCTGTCCCA GGAGGGTGAG CGGCTCATCG AGCACAAGCA CTATGCCGTG 
GACTCCATCC GCCCCAAGTG TCACGAGCTC CGCCACCTGT GCGACCAGTT CGCGGCCGAC 
GTGGAGCGGA GGAGGGGGCT GCTGGGCAAG TCGCTGGAGC TGCACGGCCT CCTGGAGGCG 
TCCATGAAGT GGTGTGACGA GGGCATCTAC CTGCTGGCAT CGCAGCCTGT GGACAAGTGC 
CAGTCCCAGG ATGGCGCAGA GGCCGCCCTC CAGGAGCTCG AGAGGTTTCT GGAGACGGGG 
GCAGAAAATA AGATCCAGGA GCTCAGCAAG ATCTTCCAGG ACTACGAGCC CATCCTCACC 
AAAGACCTGC TGGAGCATGT GCAGAGGGTC TTCCAGAAGC AGGAGAGCGT GGAGGAGATG 
TTCCACCGCA GGCAGGCCAG CCTCAAGAAG CTGGCGGCCA AGCAGACTCG GCCCGTGCAG 
CCCGTGGCCC CCCGGCCGGA AGCACTCACC AAGTCGCCCT GCCCCTCCCC AGGCATCCGG 
AGGGGTTCTG AGAACCACAG TTCCGAGGGC AGTGCACTCC GGAGGGGGCC CTACAGGAGG 
GCCAAGAGTG AGGTGAGCGA GGGCCGGCAG GGCCGTGGCG GCTCCACGGG GGACGAGGAG 
AGCCTGGCCG TCCTGCGGAG GCATGTGATG AAGGAGCTTC TGGACACAGA GCGGGTCTAC 
GTGGAGGAGC TGCTGTGCGT CCTGGAGGGC TACGCCGCGG AGATGGACAA CCCGTTAATG 
ACTCATCTCA TCTCCACTGG CCTTCAGAAC AAGAAGGACA TCCTGTTTGG AAACATGGAA 
GAAATTTACC ACTTTCATAA CAGGATATTC CTGAGGGAGC TGGAGAACTA CACGGACTGC 
CCGGAGCTGG TTGGCAGGTG CTTTCTGGAG CGGATGGAGG AGTTCCAGAT CTACGAGAAG 
TACTGTCAGA ACAAGCCGCG CTCCGAGAGC CTGTGGAGAC AGTGCTCCGA CTGCCCCTTC 
TTCCAGGAGT GCCAGAAGAA GCTGGACCAC AAGCTGAGCC TGGACTCCTA CCTGCTGAAG 
CCAGTCCAGA GGATCACCAA GTACCAGCTG CTGCTGAAGG AGATGCTTAA ATACAGCAAG 
AGTTGCGAGG GGGCGGAGGA CCTGCAGGAG GCCCTGAGCT CCATCCTGGG CATCCTCAAG 
GCCGTGAACG ACTCCATGCA CCTGATCGCC ATCACCGGCT ACGAGGGGAA CCTGAGTGAC 
CTGGGGAGGC TGCTCATGCA GGGCTCCTTC AGTGTGTGGA CCGACCACAA GAGGGGCCAC 
GCCAAGGTGA AGGACCTGGC CCGCTTCAAG CCCATGCAGC GCCACCTGTT CCTGCACGAG 
AAGGCCGTGC TCTTCTGTAA GCGGCGGGAG GAGAACGGCG AGGGCTACGA GAAGGCGCCC 
TCCTACAGCT ACAAGCAGTC CCTGAACATG ACGGCCGTCG GCATCACGGA GAACGTGAAA 
GGAGACGCCA AGAAGTTTGA GATCTGGTAC AACGCCCGGG AGGAGGTGTA CATCATCCAG 
GCACCCACTC CTGAAATCAA GGCCGCCTGG GTGAGCGAGA TCCGGAAGGT GCTGACCAGC 
CAGCTGCAGG CATGCAGAGA GGCCAGCCAG CATCGCGCCC TCGAGCAGTC CCAGAGCCTG 
CCCCTGCCCA CACCAGCTGG CACCAGTCCC TCGAAAGCGA GCACAAGAAA CATAAAAAAG 
TTAGAAGAAA GAAAAACGGA CCCGCTGAGC CTGGAGGGAT ACGGGGGGCC GACACCACAG 
CAGAGGGCCC CCGAGAAGGG CAAAGATGAC GCGGTCACTA GCTCTACCTC AGAAAGCTCT 
GCCCTTTCTA AAAAGCGCTT CACCCTGCAG AGCTTTGCTG CCCTCAAAGC CCAGAAAGCT 
TCTCCCACCA GTCCTGACAA AAAAGCTAAG CGACACCAAG TAAAGAGCGA CCCCACGCCT 
TTTGGGTTAC GAGGCTGGAG CAAAACGTCC CACCCATCAG AGACGCCTGA GGACAACGAT 
GGCTGGTCCA GCACCGAGGA GCCCGTCAAC TCCTCGGACG CGGAGGAGGA GGGCAGAGCG 
GGCCCCGGGA AGCTGGTCCC CGGGAGGTAC ACCGTCACCA GGCTGGACGA GAAGGGGGTC 
TCCGACGTGC TGGCCCTGAG AAGCGGGGAC GAGGTGGAGC TCGTGCAGGA GGGAGATGAG 
GGCCTCTGGT GCGTGCGGAA CCTGAGCTCC GGCGTCGAGG GCTGGGTGCC CGCCCACAGC 
CTGTCCACTC TCCTCTGCCA GGGTGGCCCC GTGGGGTGCC TGAGCAGCCC AGAGTCCAGT 
GCGGGTTCCG CGGTGCTGAG CCCCTCGTCC AGCTGCAGTG AGAGCTGCAC GGCCGCCCTC 
GCTGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855946.1
CDS	19..3696
Translation	MSFPAADAKRGNVWTQGHTLKKTKGSSGRNSRGAQSTGGCTETLKGVSLGCFLSFGKSMHPKLLNTALAVDEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKDDAVTSSTSESSALSKKRFTLQSFAALKAQKASPTSPDKKAKRHQVKSDPTPFGLRGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPAHSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_025000178.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000178.1

ORF Insert Sequence:

ATGAGCTTCC CAGCAGCTGA TGCAAAAAGG GGAAACGTTT GGACGCAAGG TCACACGCTC 
AAAAAAACGA AAGGTTCTTC CGGAAGGAAT TCCAGAGGAG CGCAGTCGAC CGGCGGGTGC 
ACTGAAACCC TCAAGGGGGT GTCTTTGGGA TGTTTCCTTT CTTTTGGGAA ATCGATGCAC 
CCGAAATTGT TGAATACGGC ACTGGCTGTG GATGAGATCA TGCACCACGA CATCAGCCCG 
CTCTGTGCCG CCGACATCCG GGACCAGCTG CAGAAGCGCT TCGCTTACCT GTCAGGCGGG 
CGGGGCCAGG ACGGCAGCCC GGTCATCACC TTTCCCGACT ACCCGGCCTT CGGAGACGTC 
CCAGACCAGG ACTTCCAGAA TGTCATGACC TACCTCACCA GCATCCCCAG CCTGCAGGAC 
GCTGGCATCG GGTTCATCCT GGTCATAGAC CGCAGGCAGG ACAGATGGAC CTCGGTCAAG 
GCGTCCATCC TGAGAATAGC GGCATCCTTC CCCGCAAACC TGCAGCTCGT CCTCGTCCTG 
CGGCCAACCG GGTTCTTCCA GAGGACACTC TCCGACCTCG CCTTCAGGTT CAACAGAGAT 
GACTTTAAGA TGAAGGTGCC GGTCATAATG CTGAGCTCAG TCCCAGAACT ACACGGCTAC 
ATCGACAAGT CCCAGCTGAC GGAGGACCTG GGTGGGACGC TGGCCTACTG CCACTCCAGG 
TGGCTCCGCC ATCGCACAGC CATCGAGAGC TTCGCCCTCC TGGTCAAGCA GACGGCGCAG 
ATGCTGCAGA CCTTTGGGAC CGAGCTGGCC GAGACGGAGC TGCCCAATGA CGTGCAGTCC 
ACGAGCTCCG TGCTCCTCGC CCACACGGAG AAGAAGGACA GAGCCAAGGA GGATATGAGG 
CTGGCGCTTG TCGAGGGGCG GCGCGTCCTG GAGAGCATCC ACGAGCCACT GGCCCGGAGC 
CCAGATCAGA GCCCCAACCA GGACCAACTG GACAGTCAGA GCACCGTGCA CAGGCTCCTG 
GCCCAGCTGA ACGAGACCGA GGCCGCCTTT GACGAGTTCT GGGCAAAACA TCAGCAAAAA 
CTGGAGCAGT GCCTGCAGCT CCGGCATTTC GAGCAGGACT TCCGAGAGGT CAAATCGGCC 
CTGGACGTGC TGGCCCAGAA GGTCACGACT TTCACCGACG TGGGCAACAG CCTGGCACAC 
GTGCAGCACC TCCTGAAGGA CCTGACCAGC TTCGAGGAGA AGTCCAGTGC CGCCGTGCAG 
CGGGCCCGCA CGCTGTCCCA GGAGGGTGAG CGGCTCATCG AGCACAAGCA CTATGCCGTG 
GACTCCATCC GCCCCAAGTG TCACGAGCTC CGCCACCTGT GCGACCAGTT CGCGGCCGAC 
GTGGAGCGGA GGAGGGGGCT GCTGGGCAAG TCGCTGGAGC TGCACGGCCT CCTGGAGGCG 
TCCATGAAGT GGTGTGACGA GGGCATCTAC CTGCTGGCAT CGCAGCCTGT GGACAAGTGC 
CAGTCCCAGG ATGGCGCAGA GGCCGCCCTC CAGGAGCTCG AGAGGTTTCT GGAGACGGGG 
GCAGAAAATA AGATCCAGGA GCTCAGCAAG ATCTTCCAGG ACTACGAGCC CATCCTCACC 
AAAGACCTGC TGGAGCATGT GCAGAGGGTC TTCCAGAAGC AGGAGAGCGT GGAGGAGATG 
TTCCACCGCA GGCAGGCCAG CCTCAAGAAG CTGGCGGCCA AGCAGACTCG GCCCGTGCAG 
CCCGTGGCCC CCCGGCCGGA AGCACTCACC AAGTCGCCCT GCCCCTCCCC AGGCATCCGG 
AGGGGTTCTG AGAACCACAG TTCCGAGGGC AGTGCACTCC GGAGGGGGCC CTACAGGAGG 
GCCAAGAGTG AGGTGAGCGA GGGCCGGCAG GGCCGTGGCG GCTCCACGGG GGACGAGGAG 
AGCCTGGCCG TCCTGCGGAG GCATGTGATG AAGGAGCTTC TGGACACAGA GCGGGTCTAC 
GTGGAGGAGC TGCTGTGCGT CCTGGAGGGC TACGCCGCGG AGATGGACAA CCCGTTAATG 
ACTCATCTCA TCTCCACTGG CCTTCAGAAC AAGAAGGACA TCCTGTTTGG AAACATGGAA 
GAAATTTACC ACTTTCATAA CAGGATATTC CTGAGGGAGC TGGAGAACTA CACGGACTGC 
CCGGAGCTGG TTGGCAGGTG CTTTCTGGAG CGGATGGAGG AGTTCCAGAT CTACGAGAAG 
TACTGTCAGA ACAAGCCGCG CTCCGAGAGC CTGTGGAGAC AGTGCTCCGA CTGCCCCTTC 
TTCCAGGAGT GCCAGAAGAA GCTGGACCAC AAGCTGAGCC TGGACTCCTA CCTGCTGAAG 
CCAGTCCAGA GGATCACCAA GTACCAGCTG CTGCTGAAGG AGATGCTTAA ATACAGCAAG 
AGTTGCGAGG GGGCGGAGGA CCTGCAGGAG GCCCTGAGCT CCATCCTGGG CATCCTCAAG 
GCCGTGAACG ACTCCATGCA CCTGATCGCC ATCACCGGCT ACGAGGGGAA CCTGAGTGAC 
CTGGGGAGGC TGCTCATGCA GGGCTCCTTC AGTGTGTGGA CCGACCACAA GAGGGGCCAC 
GCCAAGGTGA AGGACCTGGC CCGCTTCAAG CCCATGCAGC GCCACCTGTT CCTGCACGAG 
AAGGCCGTGC TCTTCTGTAA GCGGCGGGAG GAGAACGGCG AGGGCTACGA GAAGGCGCCC 
TCCTACAGCT ACAAGCAGTC CCTGAACATG ACGGCCGTCG GCATCACGGA GAACGTGAAA 
GGAGACGCCA AGAAGTTTGA GATCTGGTAC AACGCCCGGG AGGAGGTGTA CATCATCCAG 
GCACCCACTC CTGAAATCAA GGCCGCCTGG GTGAGCGAGA TCCGGAAGGT GCTGACCAGC 
CAGCTGCAGG CATGCAGAGA GGCCAGCCAG CATCGCGCCC TCGAGCAGTC CCAGAGCCTG 
CCCCTGCCCA CACCAGCTGG CACCAGTCCC TCGAAAGCGA GCACAAGAAA CATAAAAAAG 
TTAGAAGAAA GAAAAACGGA CCCGCTGAGC CTGGAGGGAT ACGGGGGGCC GACACCACAG 
CAGAGGGCCC CCGAGAAGGG CAAAGATGAC GCGGTCACTA GCTCTACCTC AGAAAGCTCT 
GCCCTTTCTA AAAAGCGCTT CACCCTGCAG AGCTTTGCTG CCCTCAAAGC CCAGAAAGCT 
TCTCCCACCA GTCCTGACAA AAAAGCTAAG CGACACCAAG TAAAGAGCGA CCCCACGCCT 
TTTGGGTTAC GAGGCTGGAG CAAAACGTCC CACCCATCAG AGACGCCTGA GGACAACGAT 
GGCTGGTCCA GCACCGAGGA GCCCGTCAAC TCCTCGGACG CGGAGGAGGA GGGCAGAGCG 
GGCCCCGGGA AGCTGGTCCC CGGGAGGTAC ACCGTCACCA GGCTGGACGA GAAGGGGGTC 
TCCGACGTGC TGGCCCTGAG AAGCGGGGAC GAGGTGGAGC TCGTGCAGGA GGGAGATGAG 
GGCCTCTGGT GCGTGCGGAA CCTGAGCTCC GGCGTCGAGG GCTGGGTGCC CGCCCACAGC 
CTGTCCACTC TCCTCTGCCA GGGTGGCCCC GTGGGGTGCC TGAGCAGCCC AGAGTCCAGT 
GCGGGTTCCG CGGTGCTGAG CCCCTCGTCC AGCTGCAGTG AGAGCTGCAC GGCCGCCCTC 
GCTGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_025000179.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855947.1 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa221356
Clone ID Related Accession (Same CDS sequence)	XM_025000179.1
Accession Version	XM_025000179.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCC CAGCAGCTGA TGCAAAAAGG GGAAACGTTT GGACGCAAGG TCACACGCTC 
AAAAAAACGA AAGGTTCTTC CGGAAGGAAT TCCAGAGGAG CGCAGTCGAC CGGCGGGTGC 
ACTGAAACCC TCAAGGGGGT GTCTTTGGGA TGTTTCCTTT CTTTTGGGAA ATCGATGCAC 
CCGAAATTGT TGAATACGGC ACTGGCTGTG GATGAGATCA TGCACCACGA CATCAGCCCG 
CTCTGTGCCG CCGACATCCG GGACCAGCTG CAGAAGCGCT TCGCTTACCT GTCAGGCGGG 
CGGGGCCAGG ACGGCAGCCC GGTCATCACC TTTCCCGACT ACCCGGCCTT CGGAGACGTC 
CCAGACCAGG ACTTCCAGAA TGTCATGACC TACCTCACCA GCATCCCCAG CCTGCAGGAC 
GCTGGCATCG GGTTCATCCT GGTCATAGAC CGCAGGCAGG ACAGATGGAC CTCGGTCAAG 
GCGTCCATCC TGAGAATAGC GGCATCCTTC CCCGCAAACC TGCAGCTCGT CCTCGTCCTG 
CGGCCAACCG GGTTCTTCCA GAGGACACTC TCCGACCTCG CCTTCAGGTT CAACAGAGAT 
GACTTTAAGA TGAAGGTGCC GGTCATAATG CTGAGCTCAG TCCCAGAACT ACACGGCTAC 
ATCGACAAGT CCCAGCTGAC GGAGGACCTG GGTGGGACGC TGGCCTACTG CCACTCCAGG 
TGGCTCCGCC ATCGCACAGC CATCGAGAGC TTCGCCCTCC TGGTCAAGCA GACGGCGCAG 
ATGCTGCAGA CCTTTGGGAC CGAGCTGGCC GAGACGGAGC TGCCCAATGA CGTGCAGTCC 
ACGAGCTCCG TGCTCCTCGC CCACACGGAG AAGAAGGACA GAGCCAAGGA GGATATGAGG 
CTGGCGCTTG TCGAGGGGCG GCGCGTCCTG GAGAGCATCC ACGAGCCACT GGCCCGGAGC 
CCAGATCAGA GCCCCAACCA GGACCAACTG GACAGTCAGA GCACCGTGCA CAGGCTCCTG 
GCCCAGCTGA ACGAGACCGA GGCCGCCTTT GACGAGTTCT GGGCAAAACA TCAGCAAAAA 
CTGGAGCAGT GCCTGCAGCT CCGGCATTTC GAGCAGGACT TCCGAGAGGT CAAATCGGCC 
CTGGACGTGC TGGCCCAGAA GGTCACGACT TTCACCGACG TGGGCAACAG CCTGGCACAC 
GTGCAGCACC TCCTGAAGGA CCTGACCAGC TTCGAGGAGA AGTCCAGTGC CGCCGTGCAG 
CGGGCCCGCA CGCTGTCCCA GGAGGGTGAG CGGCTCATCG AGCACAAGCA CTATGCCGTG 
GACTCCATCC GCCCCAAGTG TCACGAGCTC CGCCACCTGT GCGACCAGTT CGCGGCCGAC 
GTGGAGCGGA GGAGGGGGCT GCTGGGCAAG TCGCTGGAGC TGCACGGCCT CCTGGAGGCG 
TCCATGAAGT GGTGTGACGA GGGCATCTAC CTGCTGGCAT CGCAGCCTGT GGACAAGTGC 
CAGTCCCAGG ATGGCGCAGA GGCCGCCCTC CAGGAGCTCG AGAGGTTTCT GGAGACGGGG 
GCAGAAAATA AGATCCAGGA GCTCAGCAAG ATCTTCCAGG ACTACGAGCC CATCCTCACC 
AAAGACCTGC TGGAGCATGT GCAGAGGGTC TTCCAGAAGC AGGAGAGCGT GGAGGAGATG 
TTCCACCGCA GGCAGGCCAG CCTCAAGAAG CTGGCGGCCA AGCAGACTCG GCCCGTGCAG 
CCCGTGGCCC CCCGGCCGGA AGCACTCACC AAGTCGCCCT GCCCCTCCCC AGGCATCCGG 
AGGGGTTCTG AGAACCACAG TTCCGAGGGC AGTGCACTCC GGAGGGGGCC CTACAGGAGG 
GCCAAGAGTG AGGTGAGCGA GGGCCGGCAG GGCCGTGGCG GCTCCACGGG GGACGAGGAG 
AGCCTGGCCG TCCTGCGGAG GCATGTGATG AAGGAGCTTC TGGACACAGA GCGGGTCTAC 
GTGGAGGAGC TGCTGTGCGT CCTGGAGGGC TACGCCGCGG AGATGGACAA CCCGTTAATG 
ACTCATCTCA TCTCCACTGG CCTTCAGAAC AAGAAGGACA TCCTGTTTGG AAACATGGAA 
GAAATTTACC ACTTTCATAA CAGGATATTC CTGAGGGAGC TGGAGAACTA CACGGACTGC 
CCGGAGCTGG TTGGCAGGTG CTTTCTGGAG CGGATGGAGG AGTTCCAGAT CTACGAGAAG 
TACTGTCAGA ACAAGCCGCG CTCCGAGAGC CTGTGGAGAC AGTGCTCCGA CTGCCCCTTC 
TTCCAGGAGT GCCAGAAGAA GCTGGACCAC AAGCTGAGCC TGGACTCCTA CCTGCTGAAG 
CCAGTCCAGA GGATCACCAA GTACCAGCTG CTGCTGAAGG AGATGCTTAA ATACAGCAAG 
AGTTGCGAGG GGGCGGAGGA CCTGCAGGAG GCCCTGAGCT CCATCCTGGG CATCCTCAAG 
GCCGTGAACG ACTCCATGCA CCTGATCGCC ATCACCGGCT ACGAGGGGAA CCTGAGTGAC 
CTGGGGAGGC TGCTCATGCA GGGCTCCTTC AGTGTGTGGA CCGACCACAA GAGGGGCCAC 
GCCAAGGTGA AGGACCTGGC CCGCTTCAAG CCCATGCAGC GCCACCTGTT CCTGCACGAG 
AAGGCCGTGC TCTTCTGTAA GCGGCGGGAG GAGAACGGCG AGGGCTACGA GAAGGCGCCC 
TCCTACAGCT ACAAGCAGTC CCTGAACATG ACGGCCGTCG GCATCACGGA GAACGTGAAA 
GGAGACGCCA AGAAGTTTGA GATCTGGTAC AACGCCCGGG AGGAGGTGTA CATCATCCAG 
GCACCCACTC CTGAAATCAA GGCCGCCTGG GTGAGCGAGA TCCGGAAGGT GCTGACCAGC 
CAGCTGCAGG CATGCAGAGA GGCCAGCCAG CATCGCGCCC TCGAGCAGTC CCAGAGCCTG 
CCCCTGCCCA CACCAGCTGG CACCAGTCCC TCGAAAGCGA GCACAAGAAA CATAAAAAAG 
TTAGAAGAAA GAAAAACGGA CCCGCTGAGC CTGGAGGGAT ACGGGGGGCC GACACCACAG 
CAGAGGGCCC CCGAGAAGGG CAAAGGCTGG AGCAAAACGT CCCACCCATC AGAGACGCCT 
GAGGACAACG ATGGCTGGTC CAGCACCGAG GAGCCCGTCA ACTCCTCGGA CGCGGAGGAG 
GAGGGCAGAG CGGGCCCCGG GAAGCTGGTC CCCGGGAGGT ACACCGTCAC CAGGCTGGAC 
GAGAAGGGGG TCTCCGACGT GCTGGCCCTG AGAAGCGGGG ACGAGGTGGA GCTCGTGCAG 
GAGGGAGATG AGGGCCTCTG GTGCGTGCGG AACCTGAGCT CCGGCGTCGA GGGCTGGGTG 
CCCGCCCACA GCCTGTCCAC TCTCCTCTGC CAGGGTGGCC CCGTGGGGTG CCTGAGCAGC 
CCAGAGTCCA GTGCGGGTTC CGCGGTGCTG AGCCCCTCGT CCAGCTGCAG TGAGAGCTGC 
ACGGCCGCCC TCGCTGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855947.1
CDS	14..3523
Translation	MSFPAADAKRGNVWTQGHTLKKTKGSSGRNSRGAQSTGGCTETLKGVSLGCFLSFGKSMHPKLLNTALAVDEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPAHSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_025000179.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000179.1

ORF Insert Sequence:

ATGAGCTTCC CAGCAGCTGA TGCAAAAAGG GGAAACGTTT GGACGCAAGG TCACACGCTC 
AAAAAAACGA AAGGTTCTTC CGGAAGGAAT TCCAGAGGAG CGCAGTCGAC CGGCGGGTGC 
ACTGAAACCC TCAAGGGGGT GTCTTTGGGA TGTTTCCTTT CTTTTGGGAA ATCGATGCAC 
CCGAAATTGT TGAATACGGC ACTGGCTGTG GATGAGATCA TGCACCACGA CATCAGCCCG 
CTCTGTGCCG CCGACATCCG GGACCAGCTG CAGAAGCGCT TCGCTTACCT GTCAGGCGGG 
CGGGGCCAGG ACGGCAGCCC GGTCATCACC TTTCCCGACT ACCCGGCCTT CGGAGACGTC 
CCAGACCAGG ACTTCCAGAA TGTCATGACC TACCTCACCA GCATCCCCAG CCTGCAGGAC 
GCTGGCATCG GGTTCATCCT GGTCATAGAC CGCAGGCAGG ACAGATGGAC CTCGGTCAAG 
GCGTCCATCC TGAGAATAGC GGCATCCTTC CCCGCAAACC TGCAGCTCGT CCTCGTCCTG 
CGGCCAACCG GGTTCTTCCA GAGGACACTC TCCGACCTCG CCTTCAGGTT CAACAGAGAT 
GACTTTAAGA TGAAGGTGCC GGTCATAATG CTGAGCTCAG TCCCAGAACT ACACGGCTAC 
ATCGACAAGT CCCAGCTGAC GGAGGACCTG GGTGGGACGC TGGCCTACTG CCACTCCAGG 
TGGCTCCGCC ATCGCACAGC CATCGAGAGC TTCGCCCTCC TGGTCAAGCA GACGGCGCAG 
ATGCTGCAGA CCTTTGGGAC CGAGCTGGCC GAGACGGAGC TGCCCAATGA CGTGCAGTCC 
ACGAGCTCCG TGCTCCTCGC CCACACGGAG AAGAAGGACA GAGCCAAGGA GGATATGAGG 
CTGGCGCTTG TCGAGGGGCG GCGCGTCCTG GAGAGCATCC ACGAGCCACT GGCCCGGAGC 
CCAGATCAGA GCCCCAACCA GGACCAACTG GACAGTCAGA GCACCGTGCA CAGGCTCCTG 
GCCCAGCTGA ACGAGACCGA GGCCGCCTTT GACGAGTTCT GGGCAAAACA TCAGCAAAAA 
CTGGAGCAGT GCCTGCAGCT CCGGCATTTC GAGCAGGACT TCCGAGAGGT CAAATCGGCC 
CTGGACGTGC TGGCCCAGAA GGTCACGACT TTCACCGACG TGGGCAACAG CCTGGCACAC 
GTGCAGCACC TCCTGAAGGA CCTGACCAGC TTCGAGGAGA AGTCCAGTGC CGCCGTGCAG 
CGGGCCCGCA CGCTGTCCCA GGAGGGTGAG CGGCTCATCG AGCACAAGCA CTATGCCGTG 
GACTCCATCC GCCCCAAGTG TCACGAGCTC CGCCACCTGT GCGACCAGTT CGCGGCCGAC 
GTGGAGCGGA GGAGGGGGCT GCTGGGCAAG TCGCTGGAGC TGCACGGCCT CCTGGAGGCG 
TCCATGAAGT GGTGTGACGA GGGCATCTAC CTGCTGGCAT CGCAGCCTGT GGACAAGTGC 
CAGTCCCAGG ATGGCGCAGA GGCCGCCCTC CAGGAGCTCG AGAGGTTTCT GGAGACGGGG 
GCAGAAAATA AGATCCAGGA GCTCAGCAAG ATCTTCCAGG ACTACGAGCC CATCCTCACC 
AAAGACCTGC TGGAGCATGT GCAGAGGGTC TTCCAGAAGC AGGAGAGCGT GGAGGAGATG 
TTCCACCGCA GGCAGGCCAG CCTCAAGAAG CTGGCGGCCA AGCAGACTCG GCCCGTGCAG 
CCCGTGGCCC CCCGGCCGGA AGCACTCACC AAGTCGCCCT GCCCCTCCCC AGGCATCCGG 
AGGGGTTCTG AGAACCACAG TTCCGAGGGC AGTGCACTCC GGAGGGGGCC CTACAGGAGG 
GCCAAGAGTG AGGTGAGCGA GGGCCGGCAG GGCCGTGGCG GCTCCACGGG GGACGAGGAG 
AGCCTGGCCG TCCTGCGGAG GCATGTGATG AAGGAGCTTC TGGACACAGA GCGGGTCTAC 
GTGGAGGAGC TGCTGTGCGT CCTGGAGGGC TACGCCGCGG AGATGGACAA CCCGTTAATG 
ACTCATCTCA TCTCCACTGG CCTTCAGAAC AAGAAGGACA TCCTGTTTGG AAACATGGAA 
GAAATTTACC ACTTTCATAA CAGGATATTC CTGAGGGAGC TGGAGAACTA CACGGACTGC 
CCGGAGCTGG TTGGCAGGTG CTTTCTGGAG CGGATGGAGG AGTTCCAGAT CTACGAGAAG 
TACTGTCAGA ACAAGCCGCG CTCCGAGAGC CTGTGGAGAC AGTGCTCCGA CTGCCCCTTC 
TTCCAGGAGT GCCAGAAGAA GCTGGACCAC AAGCTGAGCC TGGACTCCTA CCTGCTGAAG 
CCAGTCCAGA GGATCACCAA GTACCAGCTG CTGCTGAAGG AGATGCTTAA ATACAGCAAG 
AGTTGCGAGG GGGCGGAGGA CCTGCAGGAG GCCCTGAGCT CCATCCTGGG CATCCTCAAG 
GCCGTGAACG ACTCCATGCA CCTGATCGCC ATCACCGGCT ACGAGGGGAA CCTGAGTGAC 
CTGGGGAGGC TGCTCATGCA GGGCTCCTTC AGTGTGTGGA CCGACCACAA GAGGGGCCAC 
GCCAAGGTGA AGGACCTGGC CCGCTTCAAG CCCATGCAGC GCCACCTGTT CCTGCACGAG 
AAGGCCGTGC TCTTCTGTAA GCGGCGGGAG GAGAACGGCG AGGGCTACGA GAAGGCGCCC 
TCCTACAGCT ACAAGCAGTC CCTGAACATG ACGGCCGTCG GCATCACGGA GAACGTGAAA 
GGAGACGCCA AGAAGTTTGA GATCTGGTAC AACGCCCGGG AGGAGGTGTA CATCATCCAG 
GCACCCACTC CTGAAATCAA GGCCGCCTGG GTGAGCGAGA TCCGGAAGGT GCTGACCAGC 
CAGCTGCAGG CATGCAGAGA GGCCAGCCAG CATCGCGCCC TCGAGCAGTC CCAGAGCCTG 
CCCCTGCCCA CACCAGCTGG CACCAGTCCC TCGAAAGCGA GCACAAGAAA CATAAAAAAG 
TTAGAAGAAA GAAAAACGGA CCCGCTGAGC CTGGAGGGAT ACGGGGGGCC GACACCACAG 
CAGAGGGCCC CCGAGAAGGG CAAAGGCTGG AGCAAAACGT CCCACCCATC AGAGACGCCT 
GAGGACAACG ATGGCTGGTC CAGCACCGAG GAGCCCGTCA ACTCCTCGGA CGCGGAGGAG 
GAGGGCAGAG CGGGCCCCGG GAAGCTGGTC CCCGGGAGGT ACACCGTCAC CAGGCTGGAC 
GAGAAGGGGG TCTCCGACGT GCTGGCCCTG AGAAGCGGGG ACGAGGTGGA GCTCGTGCAG 
GAGGGAGATG AGGGCCTCTG GTGCGTGCGG AACCTGAGCT CCGGCGTCGA GGGCTGGGTG 
CCCGCCCACA GCCTGTCCAC TCTCCTCTGC CAGGGTGGCC CCGTGGGGTG CCTGAGCAGC 
CCAGAGTCCA GTGCGGGTTC CGCGGTGCTG AGCCCCTCGT CCAGCTGCAG TGAGAGCTGC 
ACGGCCGCCC TCGCTGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_010810996.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010809298.2 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.

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CloneID	OBa221357
Clone ID Related Accession (Same CDS sequence)	XM_010810996.3
Accession Version	XM_010810996.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810996.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT GGCTGAGACA TGAAGAGATG GCCCTGGAGG AAATGGTGCA GAGATTGAAT 
GCGGTTTCCA AGCGCACTGA TGAGATCATG CACCACGACA TCAGCCCGCT CTGTGCCGCC 
GACATCCGGG ACCAGCTGCA GAAGCGCTTC GCTTACCTGT CAGGCGGGCG GGGCCAGGAC 
GGCAGCCCGG TCATCACCTT TCCCGACTAC CCGGCCTTCG GAGACGTCCC AGACCAGGAC 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGG 
TTCATCCTGG TCATAGACCG CAGGCAGGAC AGATGGACCT CGGTCAAGGC GTCCATCCTG 
AGAATAGCGG CATCCTTCCC CGCAAACCTG CAGCTCGTCC TCGTCCTGCG GCCAACCGGG 
TTCTTCCAGA GGACACTCTC CGACCTCGCC TTCAGGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCAGTC CCAGAACTAC ACGGCTACAT CGACAAGTCC 
CAGCTGACGG AGGACCTGGG TGGGACGCTG GCCTACTGCC ACTCCAGGTG GCTCCGCCAT 
CGCACAGCCA TCGAGAGCTT CGCCCTCCTG GTCAAGCAGA CGGCGCAGAT GCTGCAGACC 
TTTGGGACCG AGCTGGCCGA GACGGAGCTG CCCAATGACG TGCAGTCCAC GAGCTCCGTG 
CTCCTCGCCC ACACGGAGAA GAAGGACAGA GCCAAGGAGG ATATGAGGCT GGCGCTTGTC 
GAGGGGCGGC GCGTCCTGGA GAGCATCCAC GAGCCACTGG CCCGGAGCCC AGATCAGAGC 
CCCAACCAGG ACCAACTGGA CAGTCAGAGC ACCGTGCACA GGCTCCTGGC CCAGCTGAAC 
GAGACCGAGG CCGCCTTTGA CGAGTTCTGG GCAAAACATC AGCAAAAACT GGAGCAGTGC 
CTGCAGCTCC GGCATTTCGA GCAGGACTTC CGAGAGGTCA AATCGGCCCT GGACGTGCTG 
GCCCAGAAGG TCACGACTTT CACCGACGTG GGCAACAGCC TGGCACACGT GCAGCACCTC 
CTGAAGGACC TGACCAGCTT CGAGGAGAAG TCCAGTGCCG CCGTGCAGCG GGCCCGCACG 
CTGTCCCAGG AGGGTGAGCG GCTCATCGAG CACAAGCACT ATGCCGTGGA CTCCATCCGC 
CCCAAGTGTC ACGAGCTCCG CCACCTGTGC GACCAGTTCG CGGCCGACGT GGAGCGGAGG 
AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG CACGGCCTCC TGGAGGCGTC CATGAAGTGG 
TGTGACGAGG GCATCTACCT GCTGGCATCG CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT 
GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG AGGTTTCTGG AGACGGGGGC AGAAAATAAG 
ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC TACGAGCCCA TCCTCACCAA AGACCTGCTG 
GAGCATGTGC AGAGGGTCTT CCAGAAGCAG GAGAGCGTGG AGGAGATGTT CCACCGCAGG 
CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG CAGACTCGGC CCGTGCAGCC CGTGGCCCCC 
CGGCCGGAAG CACTCACCAA GTCGCCCTGC CCCTCCCCAG GCATCCGGAG GGGTTCTGAG 
AACCACAGTT CCGAGGGCAG TGCACTCCGG AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG 
GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC TCCACGGGGG ACGAGGAGAG CCTGGCCGTC 
CTGCGGAGGC ATGTGATGAA GGAGCTTCTG GACACAGAGC GGGTCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGACAACC CGTTAATGAC TCATCTCATC 
TCCACTGGCC TTCAGAACAA GAAGGACATC CTGTTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GGATATTCCT GAGGGAGCTG GAGAACTACA CGGACTGCCC GGAGCTGGTT 
GGCAGGTGCT TTCTGGAGCG GATGGAGGAG TTCCAGATCT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGCT CCGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCCTTCTT CCAGGAGTGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTGAAGGAG ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG 
GCGGAGGACC TGCAGGAGGC CCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TGATCGCCAT CACCGGCTAC GAGGGGAACC TGAGTGACCT GGGGAGGCTG 
CTCATGCAGG GCTCCTTCAG TGTGTGGACC GACCACAAGA GGGGCCACGC CAAGGTGAAG 
GACCTGGCCC GCTTCAAGCC CATGCAGCGC CACCTGTTCC TGCACGAGAA GGCCGTGCTC 
TTCTGTAAGC GGCGGGAGGA GAACGGCGAG GGCTACGAGA AGGCGCCCTC CTACAGCTAC 
AAGCAGTCCC TGAACATGAC GGCCGTCGGC ATCACGGAGA ACGTGAAAGG AGACGCCAAG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTGTACA TCATCCAGGC ACCCACTCCT 
GAAATCAAGG CCGCCTGGGT GAGCGAGATC CGGAAGGTGC TGACCAGCCA GCTGCAGGCA 
TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC GAGCAGTCCC AGAGCCTGCC CCTGCCCACA 
CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC ACAAGAAACA TAAAAAAGTT AGAAGAAAGA 
AAAACGGACC CGCTGAGCCT GGAGGGATAC GGGGGGCCGA CACCACAGCA GAGGGCCCCC 
GAGAAGGGCA AAGATGACGC GGTCACTAGC TCTACCTCAG AAAGCTCTGC CCTTTCTAAA 
AAGCGCTTCA CCCTGCAGAG CTTTGCTGCC CTCAAAGCCC AGAAAGCTTC TCCCACCAGT 
CCTGACAAAA AAGCTAAGCG ACACCAAGTA AAGAGCGACC CCACGCCTTT TGGGTTACGA 
GGCTGGAGCA AAACGTCCCA CCCATCAGAG ACGCCTGAGG ACAACGATGG CTGGTCCAGC 
ACCGAGGAGC CCGTCAACTC CTCGGACGCG GAGGAGGAGG GCAGAGCGGG CCCCGGGAAG 
CTGGTCCCCG GGAGGTACAC CGTCACCAGG CTGGACGAGA AGGGGGTCTC CGACGTGCTG 
GCCCTGAGAA GCGGGGACGA GGTGGAGCTC GTGCAGGAGG GAGATGAGGG CCTCTGGTGC 
GTGCGGAACC TGAGCTCCGG CGTCGAGGGC TGGGTGCCCG CCCACAGCCT GTCCACTCTC 
CTCTGCCAGG GTGGCCCCGT GGGGTGCCTG AGCAGCCCAG AGTCCAGTGC GGGTTCCGCG 
GTGCTGAGCC CCTCGTCCAG CTGCAGTGAG AGCTGCACGG CCGCCCTCGC TGACCTGCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010809298.2
CDS	79..3624
Translation	MRFWLRHEEMALEEMVQRLNAVSKRTDEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKDDAVTSSTSESSALSKKRFTLQSFAALKAQKASPTSPDKKAKRHQVKSDPTPFGLRGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPAHSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_010810996.3
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810996.3

ORF Insert Sequence:

ATGAGGTTCT GGCTGAGACA TGAAGAGATG GCCCTGGAGG AAATGGTGCA GAGATTGAAT 
GCGGTTTCCA AGCGCACTGA TGAGATCATG CACCACGACA TCAGCCCGCT CTGTGCCGCC 
GACATCCGGG ACCAGCTGCA GAAGCGCTTC GCTTACCTGT CAGGCGGGCG GGGCCAGGAC 
GGCAGCCCGG TCATCACCTT TCCCGACTAC CCGGCCTTCG GAGACGTCCC AGACCAGGAC 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGG 
TTCATCCTGG TCATAGACCG CAGGCAGGAC AGATGGACCT CGGTCAAGGC GTCCATCCTG 
AGAATAGCGG CATCCTTCCC CGCAAACCTG CAGCTCGTCC TCGTCCTGCG GCCAACCGGG 
TTCTTCCAGA GGACACTCTC CGACCTCGCC TTCAGGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCAGTC CCAGAACTAC ACGGCTACAT CGACAAGTCC 
CAGCTGACGG AGGACCTGGG TGGGACGCTG GCCTACTGCC ACTCCAGGTG GCTCCGCCAT 
CGCACAGCCA TCGAGAGCTT CGCCCTCCTG GTCAAGCAGA CGGCGCAGAT GCTGCAGACC 
TTTGGGACCG AGCTGGCCGA GACGGAGCTG CCCAATGACG TGCAGTCCAC GAGCTCCGTG 
CTCCTCGCCC ACACGGAGAA GAAGGACAGA GCCAAGGAGG ATATGAGGCT GGCGCTTGTC 
GAGGGGCGGC GCGTCCTGGA GAGCATCCAC GAGCCACTGG CCCGGAGCCC AGATCAGAGC 
CCCAACCAGG ACCAACTGGA CAGTCAGAGC ACCGTGCACA GGCTCCTGGC CCAGCTGAAC 
GAGACCGAGG CCGCCTTTGA CGAGTTCTGG GCAAAACATC AGCAAAAACT GGAGCAGTGC 
CTGCAGCTCC GGCATTTCGA GCAGGACTTC CGAGAGGTCA AATCGGCCCT GGACGTGCTG 
GCCCAGAAGG TCACGACTTT CACCGACGTG GGCAACAGCC TGGCACACGT GCAGCACCTC 
CTGAAGGACC TGACCAGCTT CGAGGAGAAG TCCAGTGCCG CCGTGCAGCG GGCCCGCACG 
CTGTCCCAGG AGGGTGAGCG GCTCATCGAG CACAAGCACT ATGCCGTGGA CTCCATCCGC 
CCCAAGTGTC ACGAGCTCCG CCACCTGTGC GACCAGTTCG CGGCCGACGT GGAGCGGAGG 
AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG CACGGCCTCC TGGAGGCGTC CATGAAGTGG 
TGTGACGAGG GCATCTACCT GCTGGCATCG CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT 
GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG AGGTTTCTGG AGACGGGGGC AGAAAATAAG 
ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC TACGAGCCCA TCCTCACCAA AGACCTGCTG 
GAGCATGTGC AGAGGGTCTT CCAGAAGCAG GAGAGCGTGG AGGAGATGTT CCACCGCAGG 
CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG CAGACTCGGC CCGTGCAGCC CGTGGCCCCC 
CGGCCGGAAG CACTCACCAA GTCGCCCTGC CCCTCCCCAG GCATCCGGAG GGGTTCTGAG 
AACCACAGTT CCGAGGGCAG TGCACTCCGG AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG 
GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC TCCACGGGGG ACGAGGAGAG CCTGGCCGTC 
CTGCGGAGGC ATGTGATGAA GGAGCTTCTG GACACAGAGC GGGTCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGACAACC CGTTAATGAC TCATCTCATC 
TCCACTGGCC TTCAGAACAA GAAGGACATC CTGTTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GGATATTCCT GAGGGAGCTG GAGAACTACA CGGACTGCCC GGAGCTGGTT 
GGCAGGTGCT TTCTGGAGCG GATGGAGGAG TTCCAGATCT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGCT CCGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCCTTCTT CCAGGAGTGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTGAAGGAG ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG 
GCGGAGGACC TGCAGGAGGC CCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TGATCGCCAT CACCGGCTAC GAGGGGAACC TGAGTGACCT GGGGAGGCTG 
CTCATGCAGG GCTCCTTCAG TGTGTGGACC GACCACAAGA GGGGCCACGC CAAGGTGAAG 
GACCTGGCCC GCTTCAAGCC CATGCAGCGC CACCTGTTCC TGCACGAGAA GGCCGTGCTC 
TTCTGTAAGC GGCGGGAGGA GAACGGCGAG GGCTACGAGA AGGCGCCCTC CTACAGCTAC 
AAGCAGTCCC TGAACATGAC GGCCGTCGGC ATCACGGAGA ACGTGAAAGG AGACGCCAAG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTGTACA TCATCCAGGC ACCCACTCCT 
GAAATCAAGG CCGCCTGGGT GAGCGAGATC CGGAAGGTGC TGACCAGCCA GCTGCAGGCA 
TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC GAGCAGTCCC AGAGCCTGCC CCTGCCCACA 
CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC ACAAGAAACA TAAAAAAGTT AGAAGAAAGA 
AAAACGGACC CGCTGAGCCT GGAGGGATAC GGGGGGCCGA CACCACAGCA GAGGGCCCCC 
GAGAAGGGCA AAGATGACGC GGTCACTAGC TCTACCTCAG AAAGCTCTGC CCTTTCTAAA 
AAGCGCTTCA CCCTGCAGAG CTTTGCTGCC CTCAAAGCCC AGAAAGCTTC TCCCACCAGT 
CCTGACAAAA AAGCTAAGCG ACACCAAGTA AAGAGCGACC CCACGCCTTT TGGGTTACGA 
GGCTGGAGCA AAACGTCCCA CCCATCAGAG ACGCCTGAGG ACAACGATGG CTGGTCCAGC 
ACCGAGGAGC CCGTCAACTC CTCGGACGCG GAGGAGGAGG GCAGAGCGGG CCCCGGGAAG 
CTGGTCCCCG GGAGGTACAC CGTCACCAGG CTGGACGAGA AGGGGGTCTC CGACGTGCTG 
GCCCTGAGAA GCGGGGACGA GGTGGAGCTC GTGCAGGAGG GAGATGAGGG CCTCTGGTGC 
GTGCGGAACC TGAGCTCCGG CGTCGAGGGC TGGGTGCCCG CCCACAGCCT GTCCACTCTC 
CTCTGCCAGG GTGGCCCCGT GGGGTGCCTG AGCAGCCCAG AGTCCAGTGC GGGTTCCGCG 
GTGCTGAGCC CCTCGTCCAG CTGCAGTGAG AGCTGCACGG CCGCCCTCGC TGACCTGCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_010810997.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010809299.2 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa221358
Clone ID Related Accession (Same CDS sequence)	XM_010810997.3
Accession Version	XM_010810997.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810997.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT GGCTGAGACA TGAAGAGATG GCCCTGGAGG AAATGGTGCA GAGATTGAAT 
GCGGTTTCCA AGCGCACTGA TGAGATCATG CACCACGACA TCAGCCCGCT CTGTGCCGCC 
GACATCCGGG ACCAGCTGCA GAAGCGCTTC GCTTACCTGT CAGGCGGGCG GGGCCAGGAC 
GGCAGCCCGG TCATCACCTT TCCCGACTAC CCGGCCTTCG GAGACGTCCC AGACCAGGAC 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGG 
TTCATCCTGG TCATAGACCG CAGGCAGGAC AGATGGACCT CGGTCAAGGC GTCCATCCTG 
AGAATAGCGG CATCCTTCCC CGCAAACCTG CAGCTCGTCC TCGTCCTGCG GCCAACCGGG 
TTCTTCCAGA GGACACTCTC CGACCTCGCC TTCAGGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCAGTC CCAGAACTAC ACGGCTACAT CGACAAGTCC 
CAGCTGACGG AGGACCTGGG TGGGACGCTG GCCTACTGCC ACTCCAGGTG GCTCCGCCAT 
CGCACAGCCA TCGAGAGCTT CGCCCTCCTG GTCAAGCAGA CGGCGCAGAT GCTGCAGACC 
TTTGGGACCG AGCTGGCCGA GACGGAGCTG CCCAATGACG TGCAGTCCAC GAGCTCCGTG 
CTCCTCGCCC ACACGGAGAA GAAGGACAGA GCCAAGGAGG ATATGAGGCT GGCGCTTGTC 
GAGGGGCGGC GCGTCCTGGA GAGCATCCAC GAGCCACTGG CCCGGAGCCC AGATCAGAGC 
CCCAACCAGG ACCAACTGGA CAGTCAGAGC ACCGTGCACA GGCTCCTGGC CCAGCTGAAC 
GAGACCGAGG CCGCCTTTGA CGAGTTCTGG GCAAAACATC AGCAAAAACT GGAGCAGTGC 
CTGCAGCTCC GGCATTTCGA GCAGGACTTC CGAGAGGTCA AATCGGCCCT GGACGTGCTG 
GCCCAGAAGG TCACGACTTT CACCGACGTG GGCAACAGCC TGGCACACGT GCAGCACCTC 
CTGAAGGACC TGACCAGCTT CGAGGAGAAG TCCAGTGCCG CCGTGCAGCG GGCCCGCACG 
CTGTCCCAGG AGGGTGAGCG GCTCATCGAG CACAAGCACT ATGCCGTGGA CTCCATCCGC 
CCCAAGTGTC ACGAGCTCCG CCACCTGTGC GACCAGTTCG CGGCCGACGT GGAGCGGAGG 
AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG CACGGCCTCC TGGAGGCGTC CATGAAGTGG 
TGTGACGAGG GCATCTACCT GCTGGCATCG CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT 
GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG AGGTTTCTGG AGACGGGGGC AGAAAATAAG 
ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC TACGAGCCCA TCCTCACCAA AGACCTGCTG 
GAGCATGTGC AGAGGGTCTT CCAGAAGCAG GAGAGCGTGG AGGAGATGTT CCACCGCAGG 
CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG CAGACTCGGC CCGTGCAGCC CGTGGCCCCC 
CGGCCGGAAG CACTCACCAA GTCGCCCTGC CCCTCCCCAG GCATCCGGAG GGGTTCTGAG 
AACCACAGTT CCGAGGGCAG TGCACTCCGG AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG 
GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC TCCACGGGGG ACGAGGAGAG CCTGGCCGTC 
CTGCGGAGGC ATGTGATGAA GGAGCTTCTG GACACAGAGC GGGTCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGACAACC CGTTAATGAC TCATCTCATC 
TCCACTGGCC TTCAGAACAA GAAGGACATC CTGTTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GGATATTCCT GAGGGAGCTG GAGAACTACA CGGACTGCCC GGAGCTGGTT 
GGCAGGTGCT TTCTGGAGCG GATGGAGGAG TTCCAGATCT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGCT CCGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCCTTCTT CCAGGAGTGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTGAAGGAG ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG 
GCGGAGGACC TGCAGGAGGC CCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TGATCGCCAT CACCGGCTAC GAGGGGAACC TGAGTGACCT GGGGAGGCTG 
CTCATGCAGG GCTCCTTCAG TGTGTGGACC GACCACAAGA GGGGCCACGC CAAGGTGAAG 
GACCTGGCCC GCTTCAAGCC CATGCAGCGC CACCTGTTCC TGCACGAGAA GGCCGTGCTC 
TTCTGTAAGC GGCGGGAGGA GAACGGCGAG GGCTACGAGA AGGCGCCCTC CTACAGCTAC 
AAGCAGTCCC TGAACATGAC GGCCGTCGGC ATCACGGAGA ACGTGAAAGG AGACGCCAAG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTGTACA TCATCCAGGC ACCCACTCCT 
GAAATCAAGG CCGCCTGGGT GAGCGAGATC CGGAAGGTGC TGACCAGCCA GCTGCAGGCA 
TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC GAGCAGTCCC AGAGCCTGCC CCTGCCCACA 
CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC ACAAGAAACA TAAAAAAGTT AGAAGAAAGA 
AAAACGGACC CGCTGAGCCT GGAGGGATAC GGGGGGCCGA CACCACAGCA GAGGGCCCCC 
GAGAAGGGCA AAGGCTGGAG CAAAACGTCC CACCCATCAG AGACGCCTGA GGACAACGAT 
GGCTGGTCCA GCACCGAGGA GCCCGTCAAC TCCTCGGACG CGGAGGAGGA GGGCAGAGCG 
GGCCCCGGGA AGCTGGTCCC CGGGAGGTAC ACCGTCACCA GGCTGGACGA GAAGGGGGTC 
TCCGACGTGC TGGCCCTGAG AAGCGGGGAC GAGGTGGAGC TCGTGCAGGA GGGAGATGAG 
GGCCTCTGGT GCGTGCGGAA CCTGAGCTCC GGCGTCGAGG GCTGGGTGCC CGCCCACAGC 
CTGTCCACTC TCCTCTGCCA GGGTGGCCCC GTGGGGTGCC TGAGCAGCCC AGAGTCCAGT 
GCGGGTTCCG CGGTGCTGAG CCCCTCGTCC AGCTGCAGTG AGAGCTGCAC GGCCGCCCTC 
GCTGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010809299.2
CDS	79..3456
Translation	MRFWLRHEEMALEEMVQRLNAVSKRTDEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPAHSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_010810997.3
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810997.3

ORF Insert Sequence:

ATGAGGTTCT GGCTGAGACA TGAAGAGATG GCCCTGGAGG AAATGGTGCA GAGATTGAAT 
GCGGTTTCCA AGCGCACTGA TGAGATCATG CACCACGACA TCAGCCCGCT CTGTGCCGCC 
GACATCCGGG ACCAGCTGCA GAAGCGCTTC GCTTACCTGT CAGGCGGGCG GGGCCAGGAC 
GGCAGCCCGG TCATCACCTT TCCCGACTAC CCGGCCTTCG GAGACGTCCC AGACCAGGAC 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGG 
TTCATCCTGG TCATAGACCG CAGGCAGGAC AGATGGACCT CGGTCAAGGC GTCCATCCTG 
AGAATAGCGG CATCCTTCCC CGCAAACCTG CAGCTCGTCC TCGTCCTGCG GCCAACCGGG 
TTCTTCCAGA GGACACTCTC CGACCTCGCC TTCAGGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCAGTC CCAGAACTAC ACGGCTACAT CGACAAGTCC 
CAGCTGACGG AGGACCTGGG TGGGACGCTG GCCTACTGCC ACTCCAGGTG GCTCCGCCAT 
CGCACAGCCA TCGAGAGCTT CGCCCTCCTG GTCAAGCAGA CGGCGCAGAT GCTGCAGACC 
TTTGGGACCG AGCTGGCCGA GACGGAGCTG CCCAATGACG TGCAGTCCAC GAGCTCCGTG 
CTCCTCGCCC ACACGGAGAA GAAGGACAGA GCCAAGGAGG ATATGAGGCT GGCGCTTGTC 
GAGGGGCGGC GCGTCCTGGA GAGCATCCAC GAGCCACTGG CCCGGAGCCC AGATCAGAGC 
CCCAACCAGG ACCAACTGGA CAGTCAGAGC ACCGTGCACA GGCTCCTGGC CCAGCTGAAC 
GAGACCGAGG CCGCCTTTGA CGAGTTCTGG GCAAAACATC AGCAAAAACT GGAGCAGTGC 
CTGCAGCTCC GGCATTTCGA GCAGGACTTC CGAGAGGTCA AATCGGCCCT GGACGTGCTG 
GCCCAGAAGG TCACGACTTT CACCGACGTG GGCAACAGCC TGGCACACGT GCAGCACCTC 
CTGAAGGACC TGACCAGCTT CGAGGAGAAG TCCAGTGCCG CCGTGCAGCG GGCCCGCACG 
CTGTCCCAGG AGGGTGAGCG GCTCATCGAG CACAAGCACT ATGCCGTGGA CTCCATCCGC 
CCCAAGTGTC ACGAGCTCCG CCACCTGTGC GACCAGTTCG CGGCCGACGT GGAGCGGAGG 
AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG CACGGCCTCC TGGAGGCGTC CATGAAGTGG 
TGTGACGAGG GCATCTACCT GCTGGCATCG CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT 
GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG AGGTTTCTGG AGACGGGGGC AGAAAATAAG 
ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC TACGAGCCCA TCCTCACCAA AGACCTGCTG 
GAGCATGTGC AGAGGGTCTT CCAGAAGCAG GAGAGCGTGG AGGAGATGTT CCACCGCAGG 
CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG CAGACTCGGC CCGTGCAGCC CGTGGCCCCC 
CGGCCGGAAG CACTCACCAA GTCGCCCTGC CCCTCCCCAG GCATCCGGAG GGGTTCTGAG 
AACCACAGTT CCGAGGGCAG TGCACTCCGG AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG 
GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC TCCACGGGGG ACGAGGAGAG CCTGGCCGTC 
CTGCGGAGGC ATGTGATGAA GGAGCTTCTG GACACAGAGC GGGTCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGACAACC CGTTAATGAC TCATCTCATC 
TCCACTGGCC TTCAGAACAA GAAGGACATC CTGTTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GGATATTCCT GAGGGAGCTG GAGAACTACA CGGACTGCCC GGAGCTGGTT 
GGCAGGTGCT TTCTGGAGCG GATGGAGGAG TTCCAGATCT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGCT CCGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCCTTCTT CCAGGAGTGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTGAAGGAG ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG 
GCGGAGGACC TGCAGGAGGC CCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TGATCGCCAT CACCGGCTAC GAGGGGAACC TGAGTGACCT GGGGAGGCTG 
CTCATGCAGG GCTCCTTCAG TGTGTGGACC GACCACAAGA GGGGCCACGC CAAGGTGAAG 
GACCTGGCCC GCTTCAAGCC CATGCAGCGC CACCTGTTCC TGCACGAGAA GGCCGTGCTC 
TTCTGTAAGC GGCGGGAGGA GAACGGCGAG GGCTACGAGA AGGCGCCCTC CTACAGCTAC 
AAGCAGTCCC TGAACATGAC GGCCGTCGGC ATCACGGAGA ACGTGAAAGG AGACGCCAAG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTGTACA TCATCCAGGC ACCCACTCCT 
GAAATCAAGG CCGCCTGGGT GAGCGAGATC CGGAAGGTGC TGACCAGCCA GCTGCAGGCA 
TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC GAGCAGTCCC AGAGCCTGCC CCTGCCCACA 
CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC ACAAGAAACA TAAAAAAGTT AGAAGAAAGA 
AAAACGGACC CGCTGAGCCT GGAGGGATAC GGGGGGCCGA CACCACAGCA GAGGGCCCCC 
GAGAAGGGCA AAGGCTGGAG CAAAACGTCC CACCCATCAG AGACGCCTGA GGACAACGAT 
GGCTGGTCCA GCACCGAGGA GCCCGTCAAC TCCTCGGACG CGGAGGAGGA GGGCAGAGCG 
GGCCCCGGGA AGCTGGTCCC CGGGAGGTAC ACCGTCACCA GGCTGGACGA GAAGGGGGTC 
TCCGACGTGC TGGCCCTGAG AAGCGGGGAC GAGGTGGAGC TCGTGCAGGA GGGAGATGAG 
GGCCTCTGGT GCGTGCGGAA CCTGAGCTCC GGCGTCGAGG GCTGGGTGCC CGCCCACAGC 
CTGTCCACTC TCCTCTGCCA GGGTGGCCCC GTGGGGTGCC TGAGCAGCCC AGAGTCCAGT 
GCGGGTTCCG CGGTGCTGAG CCCCTCGTCC AGCTGCAGTG AGAGCTGCAC GGCCGCCCTC 
GCTGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_010810999.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010809301.2 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa221359
Clone ID Related Accession (Same CDS sequence)	XM_010810999.3
Accession Version	XM_010810999.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810999.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTGA GCCTCCTCTC TCTGCTGTCG CGGGACCTGT CCACGCTCTG GCTGCTCCTG 
AAGACCCGCA CAGATGAGAT CATGCACCAC GACATCAGCC CGCTCTGTGC CGCCGACATC 
CGGGACCAGC TGCAGAAGCG CTTCGCTTAC CTGTCAGGCG GGCGGGGCCA GGACGGCAGC 
CCGGTCATCA CCTTTCCCGA CTACCCGGCC TTCGGAGACG TCCCAGACCA GGACTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGGTTCATC 
CTGGTCATAG ACCGCAGGCA GGACAGATGG ACCTCGGTCA AGGCGTCCAT CCTGAGAATA 
GCGGCATCCT TCCCCGCAAA CCTGCAGCTC GTCCTCGTCC TGCGGCCAAC CGGGTTCTTC 
CAGAGGACAC TCTCCGACCT CGCCTTCAGG TTCAACAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC AGTCCCAGAA CTACACGGCT ACATCGACAA GTCCCAGCTG 
ACGGAGGACC TGGGTGGGAC GCTGGCCTAC TGCCACTCCA GGTGGCTCCG CCATCGCACA 
GCCATCGAGA GCTTCGCCCT CCTGGTCAAG CAGACGGCGC AGATGCTGCA GACCTTTGGG 
ACCGAGCTGG CCGAGACGGA GCTGCCCAAT GACGTGCAGT CCACGAGCTC CGTGCTCCTC 
GCCCACACGG AGAAGAAGGA CAGAGCCAAG GAGGATATGA GGCTGGCGCT TGTCGAGGGG 
CGGCGCGTCC TGGAGAGCAT CCACGAGCCA CTGGCCCGGA GCCCAGATCA GAGCCCCAAC 
CAGGACCAAC TGGACAGTCA GAGCACCGTG CACAGGCTCC TGGCCCAGCT GAACGAGACC 
GAGGCCGCCT TTGACGAGTT CTGGGCAAAA CATCAGCAAA AACTGGAGCA GTGCCTGCAG 
CTCCGGCATT TCGAGCAGGA CTTCCGAGAG GTCAAATCGG CCCTGGACGT GCTGGCCCAG 
AAGGTCACGA CTTTCACCGA CGTGGGCAAC AGCCTGGCAC ACGTGCAGCA CCTCCTGAAG 
GACCTGACCA GCTTCGAGGA GAAGTCCAGT GCCGCCGTGC AGCGGGCCCG CACGCTGTCC 
CAGGAGGGTG AGCGGCTCAT CGAGCACAAG CACTATGCCG TGGACTCCAT CCGCCCCAAG 
TGTCACGAGC TCCGCCACCT GTGCGACCAG TTCGCGGCCG ACGTGGAGCG GAGGAGGGGG 
CTGCTGGGCA AGTCGCTGGA GCTGCACGGC CTCCTGGAGG CGTCCATGAA GTGGTGTGAC 
GAGGGCATCT ACCTGCTGGC ATCGCAGCCT GTGGACAAGT GCCAGTCCCA GGATGGCGCA 
GAGGCCGCCC TCCAGGAGCT CGAGAGGTTT CTGGAGACGG GGGCAGAAAA TAAGATCCAG 
GAGCTCAGCA AGATCTTCCA GGACTACGAG CCCATCCTCA CCAAAGACCT GCTGGAGCAT 
GTGCAGAGGG TCTTCCAGAA GCAGGAGAGC GTGGAGGAGA TGTTCCACCG CAGGCAGGCC 
AGCCTCAAGA AGCTGGCGGC CAAGCAGACT CGGCCCGTGC AGCCCGTGGC CCCCCGGCCG 
GAAGCACTCA CCAAGTCGCC CTGCCCCTCC CCAGGCATCC GGAGGGGTTC TGAGAACCAC 
AGTTCCGAGG GCAGTGCACT CCGGAGGGGG CCCTACAGGA GGGCCAAGAG TGAGGTGAGC 
GAGGGCCGGC AGGGCCGTGG CGGCTCCACG GGGGACGAGG AGAGCCTGGC CGTCCTGCGG 
AGGCATGTGA TGAAGGAGCT TCTGGACACA GAGCGGGTCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAC AACCCGTTAA TGACTCATCT CATCTCCACT 
GGCCTTCAGA ACAAGAAGGA CATCCTGTTT GGAAACATGG AAGAAATTTA CCACTTTCAT 
AACAGGATAT TCCTGAGGGA GCTGGAGAAC TACACGGACT GCCCGGAGCT GGTTGGCAGG 
TGCTTTCTGG AGCGGATGGA GGAGTTCCAG ATCTACGAGA AGTACTGTCA GAACAAGCCG 
CGCTCCGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCCT TCTTCCAGGA GTGCCAGAAG 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTCCA GAGGATCACC 
AAGTACCAGC TGCTGCTGAA GGAGATGCTT AAATACAGCA AGAGTTGCGA GGGGGCGGAG 
GACCTGCAGG AGGCCCTGAG CTCCATCCTG GGCATCCTCA AGGCCGTGAA CGACTCCATG 
CACCTGATCG CCATCACCGG CTACGAGGGG AACCTGAGTG ACCTGGGGAG GCTGCTCATG 
CAGGGCTCCT TCAGTGTGTG GACCGACCAC AAGAGGGGCC ACGCCAAGGT GAAGGACCTG 
GCCCGCTTCA AGCCCATGCA GCGCCACCTG TTCCTGCACG AGAAGGCCGT GCTCTTCTGT 
AAGCGGCGGG AGGAGAACGG CGAGGGCTAC GAGAAGGCGC CCTCCTACAG CTACAAGCAG 
TCCCTGAACA TGACGGCCGT CGGCATCACG GAGAACGTGA AAGGAGACGC CAAGAAGTTT 
GAGATCTGGT ACAACGCCCG GGAGGAGGTG TACATCATCC AGGCACCCAC TCCTGAAATC 
AAGGCCGCCT GGGTGAGCGA GATCCGGAAG GTGCTGACCA GCCAGCTGCA GGCATGCAGA 
GAGGCCAGCC AGCATCGCGC CCTCGAGCAG TCCCAGAGCC TGCCCCTGCC CACACCAGCT 
GGCACCAGTC CCTCGAAAGC GAGCACAAGA AACATAAAAA AGTTAGAAGA AAGAAAAACG 
GACCCGCTGA GCCTGGAGGG ATACGGGGGG CCGACACCAC AGCAGAGGGC CCCCGAGAAG 
GGCAAAGGCT GGAGCAAAAC GTCCCACCCA TCAGAGACGC CTGAGGACAA CGATGGCTGG 
TCCAGCACCG AGGAGCCCGT CAACTCCTCG GACGCGGAGG AGGAGGGCAG AGCGGGCCCC 
GGGAAGCTGG TCCCCGGGAG GTACACCGTC ACCAGGCTGG ACGAGAAGGG GGTCTCCGAC 
GTGCTGGCCC TGAGAAGCGG GGACGAGGTG GAGCTCGTGC AGGAGGGAGA TGAGGGCCTC 
TGGTGCGTGC GGAACCTGAG CTCCGGCGTC GAGGGCTGGG TGCCCGCCCA CAGCCTGTCC 
ACTCTCCTCT GCCAGGGTGG CCCCGTGGGG TGCCTGAGCA GCCCAGAGTC CAGTGCGGGT 
TCCGCGGTGC TGAGCCCCTC GTCCAGCTGC AGTGAGAGCT GCACGGCCGC CCTCGCTGAC 
CTGCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010809301.2
CDS	50..3421
Translation	MTLSLLSLLSRDLSTLWLLLKTRTDEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPAHSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_010810999.3
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810999.3

ORF Insert Sequence:

ATGACCCTGA GCCTCCTCTC TCTGCTGTCG CGGGACCTGT CCACGCTCTG GCTGCTCCTG 
AAGACCCGCA CAGATGAGAT CATGCACCAC GACATCAGCC CGCTCTGTGC CGCCGACATC 
CGGGACCAGC TGCAGAAGCG CTTCGCTTAC CTGTCAGGCG GGCGGGGCCA GGACGGCAGC 
CCGGTCATCA CCTTTCCCGA CTACCCGGCC TTCGGAGACG TCCCAGACCA GGACTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGGTTCATC 
CTGGTCATAG ACCGCAGGCA GGACAGATGG ACCTCGGTCA AGGCGTCCAT CCTGAGAATA 
GCGGCATCCT TCCCCGCAAA CCTGCAGCTC GTCCTCGTCC TGCGGCCAAC CGGGTTCTTC 
CAGAGGACAC TCTCCGACCT CGCCTTCAGG TTCAACAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC AGTCCCAGAA CTACACGGCT ACATCGACAA GTCCCAGCTG 
ACGGAGGACC TGGGTGGGAC GCTGGCCTAC TGCCACTCCA GGTGGCTCCG CCATCGCACA 
GCCATCGAGA GCTTCGCCCT CCTGGTCAAG CAGACGGCGC AGATGCTGCA GACCTTTGGG 
ACCGAGCTGG CCGAGACGGA GCTGCCCAAT GACGTGCAGT CCACGAGCTC CGTGCTCCTC 
GCCCACACGG AGAAGAAGGA CAGAGCCAAG GAGGATATGA GGCTGGCGCT TGTCGAGGGG 
CGGCGCGTCC TGGAGAGCAT CCACGAGCCA CTGGCCCGGA GCCCAGATCA GAGCCCCAAC 
CAGGACCAAC TGGACAGTCA GAGCACCGTG CACAGGCTCC TGGCCCAGCT GAACGAGACC 
GAGGCCGCCT TTGACGAGTT CTGGGCAAAA CATCAGCAAA AACTGGAGCA GTGCCTGCAG 
CTCCGGCATT TCGAGCAGGA CTTCCGAGAG GTCAAATCGG CCCTGGACGT GCTGGCCCAG 
AAGGTCACGA CTTTCACCGA CGTGGGCAAC AGCCTGGCAC ACGTGCAGCA CCTCCTGAAG 
GACCTGACCA GCTTCGAGGA GAAGTCCAGT GCCGCCGTGC AGCGGGCCCG CACGCTGTCC 
CAGGAGGGTG AGCGGCTCAT CGAGCACAAG CACTATGCCG TGGACTCCAT CCGCCCCAAG 
TGTCACGAGC TCCGCCACCT GTGCGACCAG TTCGCGGCCG ACGTGGAGCG GAGGAGGGGG 
CTGCTGGGCA AGTCGCTGGA GCTGCACGGC CTCCTGGAGG CGTCCATGAA GTGGTGTGAC 
GAGGGCATCT ACCTGCTGGC ATCGCAGCCT GTGGACAAGT GCCAGTCCCA GGATGGCGCA 
GAGGCCGCCC TCCAGGAGCT CGAGAGGTTT CTGGAGACGG GGGCAGAAAA TAAGATCCAG 
GAGCTCAGCA AGATCTTCCA GGACTACGAG CCCATCCTCA CCAAAGACCT GCTGGAGCAT 
GTGCAGAGGG TCTTCCAGAA GCAGGAGAGC GTGGAGGAGA TGTTCCACCG CAGGCAGGCC 
AGCCTCAAGA AGCTGGCGGC CAAGCAGACT CGGCCCGTGC AGCCCGTGGC CCCCCGGCCG 
GAAGCACTCA CCAAGTCGCC CTGCCCCTCC CCAGGCATCC GGAGGGGTTC TGAGAACCAC 
AGTTCCGAGG GCAGTGCACT CCGGAGGGGG CCCTACAGGA GGGCCAAGAG TGAGGTGAGC 
GAGGGCCGGC AGGGCCGTGG CGGCTCCACG GGGGACGAGG AGAGCCTGGC CGTCCTGCGG 
AGGCATGTGA TGAAGGAGCT TCTGGACACA GAGCGGGTCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAC AACCCGTTAA TGACTCATCT CATCTCCACT 
GGCCTTCAGA ACAAGAAGGA CATCCTGTTT GGAAACATGG AAGAAATTTA CCACTTTCAT 
AACAGGATAT TCCTGAGGGA GCTGGAGAAC TACACGGACT GCCCGGAGCT GGTTGGCAGG 
TGCTTTCTGG AGCGGATGGA GGAGTTCCAG ATCTACGAGA AGTACTGTCA GAACAAGCCG 
CGCTCCGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCCT TCTTCCAGGA GTGCCAGAAG 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTCCA GAGGATCACC 
AAGTACCAGC TGCTGCTGAA GGAGATGCTT AAATACAGCA AGAGTTGCGA GGGGGCGGAG 
GACCTGCAGG AGGCCCTGAG CTCCATCCTG GGCATCCTCA AGGCCGTGAA CGACTCCATG 
CACCTGATCG CCATCACCGG CTACGAGGGG AACCTGAGTG ACCTGGGGAG GCTGCTCATG 
CAGGGCTCCT TCAGTGTGTG GACCGACCAC AAGAGGGGCC ACGCCAAGGT GAAGGACCTG 
GCCCGCTTCA AGCCCATGCA GCGCCACCTG TTCCTGCACG AGAAGGCCGT GCTCTTCTGT 
AAGCGGCGGG AGGAGAACGG CGAGGGCTAC GAGAAGGCGC CCTCCTACAG CTACAAGCAG 
TCCCTGAACA TGACGGCCGT CGGCATCACG GAGAACGTGA AAGGAGACGC CAAGAAGTTT 
GAGATCTGGT ACAACGCCCG GGAGGAGGTG TACATCATCC AGGCACCCAC TCCTGAAATC 
AAGGCCGCCT GGGTGAGCGA GATCCGGAAG GTGCTGACCA GCCAGCTGCA GGCATGCAGA 
GAGGCCAGCC AGCATCGCGC CCTCGAGCAG TCCCAGAGCC TGCCCCTGCC CACACCAGCT 
GGCACCAGTC CCTCGAAAGC GAGCACAAGA AACATAAAAA AGTTAGAAGA AAGAAAAACG 
GACCCGCTGA GCCTGGAGGG ATACGGGGGG CCGACACCAC AGCAGAGGGC CCCCGAGAAG 
GGCAAAGGCT GGAGCAAAAC GTCCCACCCA TCAGAGACGC CTGAGGACAA CGATGGCTGG 
TCCAGCACCG AGGAGCCCGT CAACTCCTCG GACGCGGAGG AGGAGGGCAG AGCGGGCCCC 
GGGAAGCTGG TCCCCGGGAG GTACACCGTC ACCAGGCTGG ACGAGAAGGG GGTCTCCGAC 
GTGCTGGCCC TGAGAAGCGG GGACGAGGTG GAGCTCGTGC AGGAGGGAGA TGAGGGCCTC 
TGGTGCGTGC GGAACCTGAG CTCCGGCGTC GAGGGCTGGG TGCCCGCCCA CAGCCTGTCC 
ACTCTCCTCT GCCAGGGTGG CCCCGTGGGG TGCCTGAGCA GCCCAGAGTC CAGTGCGGGT 
TCCGCGGTGC TGAGCCCCTC GTCCAGCTGC AGTGAGAGCT GCACGGCCGC CCTCGCTGAC 
CTGCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_025000180.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855948.1 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X19, mRNA.

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CloneID	OBa221360
Clone ID Related Accession (Same CDS sequence)	XM_025000180.1
Accession Version	XM_025000180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGACT GTTGGAGGCT CATCCTTTGC CAGAAAACCG GAAGCGACGA TGCTTCTTCC 
TCGCGAGGGT CCCACGAGGC CGAAGAGGAG GACAGCGACC CTCGGGTGGA CGTCCCTGAC 
GTCATCAGGC TTGTTCCAGA CCCGCCGGAG GGGCCACCCC TGCCTGCAGA TGAGATCATG 
CACCACGACA TCAGCCCGCT CTGTGCCGCC GACATCCGGG ACCAGCTGCA GAAGCGCTTC 
GCTTACCTGT CAGGCGGGCG GGGCCAGGAC GGCAGCCCGG TCATCACCTT TCCCGACTAC 
CCGGCCTTCG GAGACGTCCC AGACCAGGAC TTCCAGAATG TCATGACCTA CCTCACCAGC 
ATCCCCAGCC TGCAGGACGC TGGCATCGGG TTCATCCTGG TCATAGACCG CAGGCAGGAC 
AGATGGACCT CGGTCAAGGC GTCCATCCTG AGAATAGCGG CATCCTTCCC CGCAAACCTG 
CAGCTCGTCC TCGTCCTGCG GCCAACCGGG TTCTTCCAGA GGACACTCTC CGACCTCGCC 
TTCAGGTTCA ACAGAGATGA CTTTAAGATG AAGGTGCCGG TCATAATGCT GAGCTCAGTC 
CCAGAACTAC ACGGCTACAT CGACAAGTCC CAGCTGACGG AGGACCTGGG TGGGACGCTG 
GCCTACTGCC ACTCCAGGTG GCTCCGCCAT CGCACAGCCA TCGAGAGCTT CGCCCTCCTG 
GTCAAGCAGA CGGCGCAGAT GCTGCAGACC TTTGGGACCG AGCTGGCCGA GACGGAGCTG 
CCCAATGACG TGCAGTCCAC GAGCTCCGTG CTCCTCGCCC ACACGGAGAA GAAGGACAGA 
GCCAAGGAGG ATATGAGGCT GGCGCTTGTC GAGGGGCGGC GCGTCCTGGA GAGCATCCAC 
GAGCCACTGG CCCGGAGCCC AGATCAGAGC CCCAACCAGG ACCAACTGGA CAGTCAGAGC 
ACCGTGCACA GGCTCCTGGC CCAGCTGAAC GAGACCGAGG CCGCCTTTGA CGAGTTCTGG 
GCAAAACATC AGCAAAAACT GGAGCAGTGC CTGCAGCTCC GGCATTTCGA GCAGGACTTC 
CGAGAGGTCA AATCGGCCCT GGACGTGCTG GCCCAGAAGG TCACGACTTT CACCGACGTG 
GGCAACAGCC TGGCACACGT GCAGCACCTC CTGAAGGACC TGACCAGCTT CGAGGAGAAG 
TCCAGTGCCG CCGTGCAGCG GGCCCGCACG CTGTCCCAGG AGGGTGAGCG GCTCATCGAG 
CACAAGCACT ATGCCGTGGA CTCCATCCGC CCCAAGTGTC ACGAGCTCCG CCACCTGTGC 
GACCAGTTCG CGGCCGACGT GGAGCGGAGG AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG 
CACGGCCTCC TGGAGGCGTC CATGAAGTGG TGTGACGAGG GCATCTACCT GCTGGCATCG 
CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG 
AGGTTTCTGG AGACGGGGGC AGAAAATAAG ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC 
TACGAGCCCA TCCTCACCAA AGACCTGCTG GAGCATGTGC AGAGGGTCTT CCAGAAGCAG 
GAGAGCGTGG AGGAGATGTT CCACCGCAGG CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG 
CAGACTCGGC CCGTGCAGCC CGTGGCCCCC CGGCCGGAAG CACTCACCAA GTCGCCCTGC 
CCCTCCCCAG GCATCCGGAG GGGTTCTGAG AACCACAGTT CCGAGGGCAG TGCACTCCGG 
AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC 
TCCACGGGGG ACGAGGAGAG CCTGGCCGTC CTGCGGAGGC ATGTGATGAA GGAGCTTCTG 
GACACAGAGC GGGTCTACGT GGAGGAGCTG CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG 
ATGGACAACC CGTTAATGAC TCATCTCATC TCCACTGGCC TTCAGAACAA GAAGGACATC 
CTGTTTGGAA ACATGGAAGA AATTTACCAC TTTCATAACA GGATATTCCT GAGGGAGCTG 
GAGAACTACA CGGACTGCCC GGAGCTGGTT GGCAGGTGCT TTCTGGAGCG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCGCGCT CCGAGAGCCT GTGGAGACAG 
TGCTCCGACT GCCCCTTCTT CCAGGAGTGC CAGAAGAAGC TGGACCACAA GCTGAGCCTG 
GACTCCTACC TGCTGAAGCC AGTCCAGAGG ATCACCAAGT ACCAGCTGCT GCTGAAGGAG 
ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG GCGGAGGACC TGCAGGAGGC CCTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC CGTGAACGAC TCCATGCACC TGATCGCCAT CACCGGCTAC 
GAGGGGAACC TGAGTGACCT GGGGAGGCTG CTCATGCAGG GCTCCTTCAG TGTGTGGACC 
GACCACAAGA GGGGCCACGC CAAGGTGAAG GACCTGGCCC GCTTCAAGCC CATGCAGCGC 
CACCTGTTCC TGCACGAGAA GGCCGTGCTC TTCTGTAAGC GGCGGGAGGA GAACGGCGAG 
GGCTACGAGA AGGCGCCCTC CTACAGCTAC AAGCAGTCCC TGAACATGAC GGCCGTCGGC 
ATCACGGAGA ACGTGAAAGG AGACGCCAAG AAGTTTGAGA TCTGGTACAA CGCCCGGGAG 
GAGGTGTACA TCATCCAGGC ACCCACTCCT GAAATCAAGG CCGCCTGGGT GAGCGAGATC 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCA TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC 
GAGCAGTCCC AGAGCCTGCC CCTGCCCACA CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC 
ACAAGAAACA TAAAAAAGTT AGAAGAAAGA AAAACGGACC CGCTGAGCCT GGAGGGATAC 
GGGGGGCCGA CACCACAGCA GAGGGCCCCC GAGAAGGGCA AAGGCTGGAG CAAAACGTCC 
CACCCATCAG AGACGCCTGA GGACAACGAT GGCTGGTCCA GCACCGAGGA GCCCGTCAAC 
TCCTCGGACG CGGAGGAGGA GGGCAGAGCG GGCCCCGGGA AGCTGGTCCC CGGGAGGTAC 
ACCGTCACCA GGCTGGACGA GAAGGGGGTC TCCGACGTGC TGGCCCTGAG AAGCGGGGAC 
GAGGTGGAGC TCGTGCAGGA GGGAGATGAG GGCCTCTGGT GCGTGCGGAA CCTGAGCTCC 
GGCGTCGAGG GCTGGGTGCC CGCCCACAGC CTGTCCACTC TCCTCTGCCA GGGTGGCCCC 
GTGGGGTGCC TGAGCAGCCC AGAGTCCAGT GCGGGTTCCG CGGTGCTGAG CCCCTCGTCC 
AGCTGCAGTG AGAGCTGCAC GGCCGCCCTC GCTGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855948.1
CDS	5..3472
Translation	MFDCWRLILCQKTGSDDASSSRGSHEAEEEDSDPRVDVPDVIRLVPDPPEGPPLPADEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPAHSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_025000180.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000180.1

ORF Insert Sequence:

ATGTTCGACT GTTGGAGGCT CATCCTTTGC CAGAAAACCG GAAGCGACGA TGCTTCTTCC 
TCGCGAGGGT CCCACGAGGC CGAAGAGGAG GACAGCGACC CTCGGGTGGA CGTCCCTGAC 
GTCATCAGGC TTGTTCCAGA CCCGCCGGAG GGGCCACCCC TGCCTGCAGA TGAGATCATG 
CACCACGACA TCAGCCCGCT CTGTGCCGCC GACATCCGGG ACCAGCTGCA GAAGCGCTTC 
GCTTACCTGT CAGGCGGGCG GGGCCAGGAC GGCAGCCCGG TCATCACCTT TCCCGACTAC 
CCGGCCTTCG GAGACGTCCC AGACCAGGAC TTCCAGAATG TCATGACCTA CCTCACCAGC 
ATCCCCAGCC TGCAGGACGC TGGCATCGGG TTCATCCTGG TCATAGACCG CAGGCAGGAC 
AGATGGACCT CGGTCAAGGC GTCCATCCTG AGAATAGCGG CATCCTTCCC CGCAAACCTG 
CAGCTCGTCC TCGTCCTGCG GCCAACCGGG TTCTTCCAGA GGACACTCTC CGACCTCGCC 
TTCAGGTTCA ACAGAGATGA CTTTAAGATG AAGGTGCCGG TCATAATGCT GAGCTCAGTC 
CCAGAACTAC ACGGCTACAT CGACAAGTCC CAGCTGACGG AGGACCTGGG TGGGACGCTG 
GCCTACTGCC ACTCCAGGTG GCTCCGCCAT CGCACAGCCA TCGAGAGCTT CGCCCTCCTG 
GTCAAGCAGA CGGCGCAGAT GCTGCAGACC TTTGGGACCG AGCTGGCCGA GACGGAGCTG 
CCCAATGACG TGCAGTCCAC GAGCTCCGTG CTCCTCGCCC ACACGGAGAA GAAGGACAGA 
GCCAAGGAGG ATATGAGGCT GGCGCTTGTC GAGGGGCGGC GCGTCCTGGA GAGCATCCAC 
GAGCCACTGG CCCGGAGCCC AGATCAGAGC CCCAACCAGG ACCAACTGGA CAGTCAGAGC 
ACCGTGCACA GGCTCCTGGC CCAGCTGAAC GAGACCGAGG CCGCCTTTGA CGAGTTCTGG 
GCAAAACATC AGCAAAAACT GGAGCAGTGC CTGCAGCTCC GGCATTTCGA GCAGGACTTC 
CGAGAGGTCA AATCGGCCCT GGACGTGCTG GCCCAGAAGG TCACGACTTT CACCGACGTG 
GGCAACAGCC TGGCACACGT GCAGCACCTC CTGAAGGACC TGACCAGCTT CGAGGAGAAG 
TCCAGTGCCG CCGTGCAGCG GGCCCGCACG CTGTCCCAGG AGGGTGAGCG GCTCATCGAG 
CACAAGCACT ATGCCGTGGA CTCCATCCGC CCCAAGTGTC ACGAGCTCCG CCACCTGTGC 
GACCAGTTCG CGGCCGACGT GGAGCGGAGG AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG 
CACGGCCTCC TGGAGGCGTC CATGAAGTGG TGTGACGAGG GCATCTACCT GCTGGCATCG 
CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG 
AGGTTTCTGG AGACGGGGGC AGAAAATAAG ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC 
TACGAGCCCA TCCTCACCAA AGACCTGCTG GAGCATGTGC AGAGGGTCTT CCAGAAGCAG 
GAGAGCGTGG AGGAGATGTT CCACCGCAGG CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG 
CAGACTCGGC CCGTGCAGCC CGTGGCCCCC CGGCCGGAAG CACTCACCAA GTCGCCCTGC 
CCCTCCCCAG GCATCCGGAG GGGTTCTGAG AACCACAGTT CCGAGGGCAG TGCACTCCGG 
AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC 
TCCACGGGGG ACGAGGAGAG CCTGGCCGTC CTGCGGAGGC ATGTGATGAA GGAGCTTCTG 
GACACAGAGC GGGTCTACGT GGAGGAGCTG CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG 
ATGGACAACC CGTTAATGAC TCATCTCATC TCCACTGGCC TTCAGAACAA GAAGGACATC 
CTGTTTGGAA ACATGGAAGA AATTTACCAC TTTCATAACA GGATATTCCT GAGGGAGCTG 
GAGAACTACA CGGACTGCCC GGAGCTGGTT GGCAGGTGCT TTCTGGAGCG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCGCGCT CCGAGAGCCT GTGGAGACAG 
TGCTCCGACT GCCCCTTCTT CCAGGAGTGC CAGAAGAAGC TGGACCACAA GCTGAGCCTG 
GACTCCTACC TGCTGAAGCC AGTCCAGAGG ATCACCAAGT ACCAGCTGCT GCTGAAGGAG 
ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG GCGGAGGACC TGCAGGAGGC CCTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC CGTGAACGAC TCCATGCACC TGATCGCCAT CACCGGCTAC 
GAGGGGAACC TGAGTGACCT GGGGAGGCTG CTCATGCAGG GCTCCTTCAG TGTGTGGACC 
GACCACAAGA GGGGCCACGC CAAGGTGAAG GACCTGGCCC GCTTCAAGCC CATGCAGCGC 
CACCTGTTCC TGCACGAGAA GGCCGTGCTC TTCTGTAAGC GGCGGGAGGA GAACGGCGAG 
GGCTACGAGA AGGCGCCCTC CTACAGCTAC AAGCAGTCCC TGAACATGAC GGCCGTCGGC 
ATCACGGAGA ACGTGAAAGG AGACGCCAAG AAGTTTGAGA TCTGGTACAA CGCCCGGGAG 
GAGGTGTACA TCATCCAGGC ACCCACTCCT GAAATCAAGG CCGCCTGGGT GAGCGAGATC 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCA TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC 
GAGCAGTCCC AGAGCCTGCC CCTGCCCACA CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC 
ACAAGAAACA TAAAAAAGTT AGAAGAAAGA AAAACGGACC CGCTGAGCCT GGAGGGATAC 
GGGGGGCCGA CACCACAGCA GAGGGCCCCC GAGAAGGGCA AAGGCTGGAG CAAAACGTCC 
CACCCATCAG AGACGCCTGA GGACAACGAT GGCTGGTCCA GCACCGAGGA GCCCGTCAAC 
TCCTCGGACG CGGAGGAGGA GGGCAGAGCG GGCCCCGGGA AGCTGGTCCC CGGGAGGTAC 
ACCGTCACCA GGCTGGACGA GAAGGGGGTC TCCGACGTGC TGGCCCTGAG AAGCGGGGAC 
GAGGTGGAGC TCGTGCAGGA GGGAGATGAG GGCCTCTGGT GCGTGCGGAA CCTGAGCTCC 
GGCGTCGAGG GCTGGGTGCC CGCCCACAGC CTGTCCACTC TCCTCTGCCA GGGTGGCCCC 
GTGGGGTGCC TGAGCAGCCC AGAGTCCAGT GCGGGTTCCG CGGTGCTGAG CCCCTCGTCC 
AGCTGCAGTG AGAGCTGCAC GGCCGCCCTC GCTGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_015465840.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321326.1 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa92205
Clone ID Related Accession (Same CDS sequence)	XM_015465840.1 , XM_015473940.1
Accession Version	XM_015465840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646112.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCCG CGACCTCGGG CTGCTTGCCT TCCTGGCTGA GGTGCATCAG CAGGCCGGCC 
GGCCGGCTGC AGGCCTGCCC AGAGGCCGGG AAGTGGGACA CGGACCTGTC AGTCAGCGTG 
CGCCTGCTCT TAGGCGCATT AGCAAAATCT CTGCAGGAAA CAGCGGGTGC CGAGCCAGAG 
CTCGTGCTCG GCTTAACTCT TCAACACCCG GGAGCCCGGG AAGCAGGTAG GGCCTCAGCC 
ACGGGTCAGG ATCCGCGGGG GACGTGTCCA CCGTCCCCCG GACAGTGGGG CGGGGACACA 
GTCTCTGTGC CCGCCTGGCT TCCTCCTGCC GGGCCTGCCT TTCCTCGGGA GCCCAGTTTC 
CTGACGTCCT CTCCGCTGGA TGTCGCCACA GGAGCCTGTC AGGATGAGAT CATGCACCAC 
GACATCAGCC CGCTCTGTGC CGCCGACATC CGGGACCAGC TGCAGAAGCG CTTCGCTTAC 
CTGTCAGGCG GGCGGGGCCA GGACGGCAGC CCGGTCATCA CCTTTCCCGA CTACCCGGCC 
TTCGGAGACG TCCCAGACCA GGACTTCCAG AATGTCATGA CCTACCTCAC CAGCATCCCC 
AGCCTGCAGG ACGCTGGCAT CGGGTTCATC CTGGTCATAG ACCGCAGGCA GGACAGATGG 
ACCTCGGTCA AGGCGTCCAT CCTGAGAATA GCGGCATCCT TCCCCGCAAA CCTGCAGCTC 
GTCCTCGTCC TGCGGCCAAC CGGGTTCTTC CAGAGGACAC TCTCCGACCT CGCCTTCAGG 
TTCAACAGAG ATGACTTTAA GATGAAGGTG CCGGTCATAA TGCTGAGCTC AGTCCCAGAA 
CTACACGGCT ACATCGACAA GTCCCAGCTG ACGGAGGACC TGGGTGGGAC GCTGGCCTAC 
TGCCACTCCA GGTGGCTCCG CCATCGCACA GCCATCGAGA GCTTCGCCCT CCTGGTCAAG 
CAGACGGCGC AGATGCTGCA GACCTTTGGG ACCGAGCTGG CCGAGACGGA GCTGCCCAAT 
GACGTGCAGT CCACGAGCTC CGTGCTCCTC GCCCACACGG AGAAGAAGGA CAGAGCCAAG 
GAGGATATGA GGCTGGCGCT TGTCGAGGGG CGGCGCGTCC TGGAGAGCAT CCACGAGCCA 
CTGGCCCGGA GCCCAGATCA GAGCCCCAAC CAGGACCAAC TGGACAGTCA GAGCACCGTG 
CACAGGCTCC TGGCCCAGCT GAACGAGACC GAGGCCGCCT TTGACGAGTT CTGGGCAAAA 
CATCAGCAAA AACTGGAGCA GTGCCTGCAG CTCCGGCATT TCGAGCAGGA CTTCCGAGAG 
GTCAAATCGG CCCTGGACGT GCTGGCCCAG AAGGTCACGA CTTTCACCGA CGTGGGCAAC 
AGCCTGGCAC ACGTGCAGCA CCTCCTGAAG GACCTGACCA GCTTCGAGGA GAAGTCCAGT 
GCCGCCGTGC AGCGGGCCCG CACGCTGTCC CAGGAGGGTG AGCGGCTCAT CGAGCACAAG 
CACTATGCCG TGGACTCCAT CCGCCCCAAG TGTCACGAGC TCCGCCACCT GTGCGACCAG 
TTCGCGGCCG ACGTGGAGCG GAGGAGGGGG CTGCTGGGCA AGTCGCTGGA GCTGCACGGC 
CTCCTGGAGG CGTCCATGAA GTGGTGTGAC GAGGGCATCT ACCTGCTGGC ATCGCAGCCT 
GTGGACAAGT GCCAGTCCCA GGATGGCGCA GAGGCCGCCC TCCAGGAGCT CGAGAGGTTT 
CTGGAGACGG GGGCAGAAAA TAAGATCCAG GAGCTCAGCA AGATCTTCCA GGACTACGAG 
CCCATCCTCA CCAAAGACCT GCTGGAGCAT GTGCAGAGGG TCTTCCAGAA GCAGGAGAGC 
GTGGAGGAGA TGTTCCACCG CAGGCAGGCC AGCCTCAAGA AGCTGGCGGC CAAGCAGACT 
CGGCCCGTGC AGCCCGTGGC CCCCCGGCCG GAAGCACTCA CCAAGTCGCC CTGCCCCTCC 
CCAGGCATCC GGAGGGGTTC TGAGAACCAC AGTTCCGAGG GCAGTGCACT CCGGAGGGGG 
CCCTACAGGA GGGCCAAGAG TGAGGTGAGC GAGGGCCGGC AGGGCCGTGG CGGCTCCACG 
GGGGACGAGG AGAGCCTGGC CGTCCTGCGG AGGCATGTGA TGAAGGAGCT TCTGGACACA 
GAGCGGGTCT ACGTGGAGGA GCTGCTGTGC GTCCTGGAGG GCTACGCCGC GGAGATGGAC 
AACCCGTTAA TGACTCATCT CATCTCCACT GGCCTTCAGA ACAAGAAGGA CATCCTGTTT 
GGAAACATGG AAGAAATTTA CCACTTTCAT AACAGGATAT TCCTGAGGGA GCTGGAGAAC 
TACACGGACT GCCCGGAGCT GGTTGGCAGG TGCTTTCTGG AGCGGATGGA GGAGTTCCAG 
ATCTACGAGA AGTACTGTCA GAACAAGCCG CGCTCCGAGA GCCTGTGGAG ACAGTGCTCC 
GACTGCCCCT TCTTCCAGGA GTGCCAGAAG AAGCTGGACC ACAAGCTGAG CCTGGACTCC 
TACCTGCTGA AGCCAGTCCA GAGGATCACC AAGTACCAGC TGCTGCTGAA GGAGATGCTT 
AAATACAGCA AGAGTTGCGA GGGGGCGGAG GACCTGCAGG AGGCCCTGAG CTCCATCCTG 
GGCATCCTCA AGGCCGTGAA CGACTCCATG CACCTGATCG CCATCACCGG CTACGAGGGG 
AACCTGAGTG ACCTGGGGAG GCTGCTCATG CAGGGCTCCT TCAGTGTGTG GACCGACCAC 
AAGAGGGGCC ACGCCAAGGT GAAGGACCTG GCCCGCTTCA AGCCCATGCA GCGCCACCTG 
TTCCTGCACG AGAAGGCCGT GCTCTTCTGT AAGCGGCGGG AGGAGAACGG CGAGGGCTAC 
GAGAAGGCGC CCTCCTACAG CTACAAGCAG TCCCTGAACA TGACGGCCGT CGGCATCACG 
GAGAACGTGA AAGGAGACGC CAAGAAGTTT GAGATCTGGT ACAACGCCCG GGAGGAGGTG 
TACATCATCC AGGCACCCAC TCCTGAAATC AAGGCCGCCT GGGTGAGCGA GATCCGGAAG 
GTGCTGACCA GCCAGCTGCA GGCATGCAGA GAGGCCAGCC AGCATCGCGC CCTCGAGCAG 
TCCCAGAGCC TGCCCCTGCC CACACCAGCT GGCACCAGTC CCTCGAAAGC GAGCACAAGA 
AACATAAAAA AGTTAGAAGA AAGAAAAACG GACCCGCTGA GCCTGGAGGG ATACGGGGGG 
CCGACACCAC AGCAGAGGGC CCCCGAGAAG GGCAAAGATG ACGCGGTCAC TAGCTCTACC 
TCAGAAAGCT CTGCCCTTTC TAAAAAGCGC TTCACCCTGC AGAGCTTTGC TGCCCTCAAA 
GCCCAGAAAG CTTCTCCCAC CAGTCCTGAC AAAAAAGCTA AGCGACACCA AGTAAAGAGC 
GACCCCACGC CTTTTGGGTT ACGAGGCTGG AGCAAAACGT CCCACCCATC AGAGACGCCT 
GAGGACAACG ATGGCTGGTC CAGCACCGAG GAGCCCGTCA ACTCCTCGGA CGCGGAGGAG 
GAGGGCAGAG CGGGCCCCGG GAAGCTGGTC CCCGGGAGGT ACACCGTCAC CAGGCTGGAC 
GAGAAGGGGG TCTCCGACGT GCTGGCCCTG AGAAGCGGGG ACGAGGTGGA GCTCGTGCAG 
GAGGGAGATG AGGGCCTCTG GTGCGTGCGG AACCTGAGCT CCGGCGTCGA GGGCTGGGTG 
CCCGCCCGCA GCCTGTCCAC TCTCCTCTGC CAGGGTGGCC CCGTGGGGTG CCTGAGCAGC 
CCAGAGTCCA GTGCGGGTTC CGCGGTGCTG AGCCCCTCGT CCAGCTGCAG TGAGAGCTGC 
ACGGCCGCCC TCGCTGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321326.1
CDS	120..3989
Translation	MLAATSGCLPSWLRCISRPAGRLQACPEAGKWDTDLSVSVRLLLGALAKSLQETAGAEPELVLGLTLQHPGAREAGRASATGQDPRGTCPPSPGQWGGDTVSVPAWLPPAGPAFPREPSFLTSSPLDVATGACQDEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKDDAVTSSTSESSALSKKRFTLQSFAALKAQKASPTSPDKKAKRHQVKSDPTPFGLRGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPARSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_015465840.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465840.1

ORF Insert Sequence:

ATGCTGGCCG CGACCTCGGG CTGCTTGCCT TCCTGGCTGA GGTGCATCAG CAGGCCGGCC 
GGCCGGCTGC AGGCCTGCCC AGAGGCCGGG AAGTGGGACA CGGACCTGTC AGTCAGCGTG 
CGCCTGCTCT TAGGCGCATT AGCAAAATCT CTGCAGGAAA CAGCGGGTGC CGAGCCAGAG 
CTCGTGCTCG GCTTAACTCT TCAACACCCG GGAGCCCGGG AAGCAGGTAG GGCCTCAGCC 
ACGGGTCAGG ATCCGCGGGG GACGTGTCCA CCGTCCCCCG GACAGTGGGG CGGGGACACA 
GTCTCTGTGC CCGCCTGGCT TCCTCCTGCC GGGCCTGCCT TTCCTCGGGA GCCCAGTTTC 
CTGACGTCCT CTCCGCTGGA TGTCGCCACA GGAGCCTGTC AGGATGAGAT CATGCACCAC 
GACATCAGCC CGCTCTGTGC CGCCGACATC CGGGACCAGC TGCAGAAGCG CTTCGCTTAC 
CTGTCAGGCG GGCGGGGCCA GGACGGCAGC CCGGTCATCA CCTTTCCCGA CTACCCGGCC 
TTCGGAGACG TCCCAGACCA GGACTTCCAG AATGTCATGA CCTACCTCAC CAGCATCCCC 
AGCCTGCAGG ACGCTGGCAT CGGGTTCATC CTGGTCATAG ACCGCAGGCA GGACAGATGG 
ACCTCGGTCA AGGCGTCCAT CCTGAGAATA GCGGCATCCT TCCCCGCAAA CCTGCAGCTC 
GTCCTCGTCC TGCGGCCAAC CGGGTTCTTC CAGAGGACAC TCTCCGACCT CGCCTTCAGG 
TTCAACAGAG ATGACTTTAA GATGAAGGTG CCGGTCATAA TGCTGAGCTC AGTCCCAGAA 
CTACACGGCT ACATCGACAA GTCCCAGCTG ACGGAGGACC TGGGTGGGAC GCTGGCCTAC 
TGCCACTCCA GGTGGCTCCG CCATCGCACA GCCATCGAGA GCTTCGCCCT CCTGGTCAAG 
CAGACGGCGC AGATGCTGCA GACCTTTGGG ACCGAGCTGG CCGAGACGGA GCTGCCCAAT 
GACGTGCAGT CCACGAGCTC CGTGCTCCTC GCCCACACGG AGAAGAAGGA CAGAGCCAAG 
GAGGATATGA GGCTGGCGCT TGTCGAGGGG CGGCGCGTCC TGGAGAGCAT CCACGAGCCA 
CTGGCCCGGA GCCCAGATCA GAGCCCCAAC CAGGACCAAC TGGACAGTCA GAGCACCGTG 
CACAGGCTCC TGGCCCAGCT GAACGAGACC GAGGCCGCCT TTGACGAGTT CTGGGCAAAA 
CATCAGCAAA AACTGGAGCA GTGCCTGCAG CTCCGGCATT TCGAGCAGGA CTTCCGAGAG 
GTCAAATCGG CCCTGGACGT GCTGGCCCAG AAGGTCACGA CTTTCACCGA CGTGGGCAAC 
AGCCTGGCAC ACGTGCAGCA CCTCCTGAAG GACCTGACCA GCTTCGAGGA GAAGTCCAGT 
GCCGCCGTGC AGCGGGCCCG CACGCTGTCC CAGGAGGGTG AGCGGCTCAT CGAGCACAAG 
CACTATGCCG TGGACTCCAT CCGCCCCAAG TGTCACGAGC TCCGCCACCT GTGCGACCAG 
TTCGCGGCCG ACGTGGAGCG GAGGAGGGGG CTGCTGGGCA AGTCGCTGGA GCTGCACGGC 
CTCCTGGAGG CGTCCATGAA GTGGTGTGAC GAGGGCATCT ACCTGCTGGC ATCGCAGCCT 
GTGGACAAGT GCCAGTCCCA GGATGGCGCA GAGGCCGCCC TCCAGGAGCT CGAGAGGTTT 
CTGGAGACGG GGGCAGAAAA TAAGATCCAG GAGCTCAGCA AGATCTTCCA GGACTACGAG 
CCCATCCTCA CCAAAGACCT GCTGGAGCAT GTGCAGAGGG TCTTCCAGAA GCAGGAGAGC 
GTGGAGGAGA TGTTCCACCG CAGGCAGGCC AGCCTCAAGA AGCTGGCGGC CAAGCAGACT 
CGGCCCGTGC AGCCCGTGGC CCCCCGGCCG GAAGCACTCA CCAAGTCGCC CTGCCCCTCC 
CCAGGCATCC GGAGGGGTTC TGAGAACCAC AGTTCCGAGG GCAGTGCACT CCGGAGGGGG 
CCCTACAGGA GGGCCAAGAG TGAGGTGAGC GAGGGCCGGC AGGGCCGTGG CGGCTCCACG 
GGGGACGAGG AGAGCCTGGC CGTCCTGCGG AGGCATGTGA TGAAGGAGCT TCTGGACACA 
GAGCGGGTCT ACGTGGAGGA GCTGCTGTGC GTCCTGGAGG GCTACGCCGC GGAGATGGAC 
AACCCGTTAA TGACTCATCT CATCTCCACT GGCCTTCAGA ACAAGAAGGA CATCCTGTTT 
GGAAACATGG AAGAAATTTA CCACTTTCAT AACAGGATAT TCCTGAGGGA GCTGGAGAAC 
TACACGGACT GCCCGGAGCT GGTTGGCAGG TGCTTTCTGG AGCGGATGGA GGAGTTCCAG 
ATCTACGAGA AGTACTGTCA GAACAAGCCG CGCTCCGAGA GCCTGTGGAG ACAGTGCTCC 
GACTGCCCCT TCTTCCAGGA GTGCCAGAAG AAGCTGGACC ACAAGCTGAG CCTGGACTCC 
TACCTGCTGA AGCCAGTCCA GAGGATCACC AAGTACCAGC TGCTGCTGAA GGAGATGCTT 
AAATACAGCA AGAGTTGCGA GGGGGCGGAG GACCTGCAGG AGGCCCTGAG CTCCATCCTG 
GGCATCCTCA AGGCCGTGAA CGACTCCATG CACCTGATCG CCATCACCGG CTACGAGGGG 
AACCTGAGTG ACCTGGGGAG GCTGCTCATG CAGGGCTCCT TCAGTGTGTG GACCGACCAC 
AAGAGGGGCC ACGCCAAGGT GAAGGACCTG GCCCGCTTCA AGCCCATGCA GCGCCACCTG 
TTCCTGCACG AGAAGGCCGT GCTCTTCTGT AAGCGGCGGG AGGAGAACGG CGAGGGCTAC 
GAGAAGGCGC CCTCCTACAG CTACAAGCAG TCCCTGAACA TGACGGCCGT CGGCATCACG 
GAGAACGTGA AAGGAGACGC CAAGAAGTTT GAGATCTGGT ACAACGCCCG GGAGGAGGTG 
TACATCATCC AGGCACCCAC TCCTGAAATC AAGGCCGCCT GGGTGAGCGA GATCCGGAAG 
GTGCTGACCA GCCAGCTGCA GGCATGCAGA GAGGCCAGCC AGCATCGCGC CCTCGAGCAG 
TCCCAGAGCC TGCCCCTGCC CACACCAGCT GGCACCAGTC CCTCGAAAGC GAGCACAAGA 
AACATAAAAA AGTTAGAAGA AAGAAAAACG GACCCGCTGA GCCTGGAGGG ATACGGGGGG 
CCGACACCAC AGCAGAGGGC CCCCGAGAAG GGCAAAGATG ACGCGGTCAC TAGCTCTACC 
TCAGAAAGCT CTGCCCTTTC TAAAAAGCGC TTCACCCTGC AGAGCTTTGC TGCCCTCAAA 
GCCCAGAAAG CTTCTCCCAC CAGTCCTGAC AAAAAAGCTA AGCGACACCA AGTAAAGAGC 
GACCCCACGC CTTTTGGGTT ACGAGGCTGG AGCAAAACGT CCCACCCATC AGAGACGCCT 
GAGGACAACG ATGGCTGGTC CAGCACCGAG GAGCCCGTCA ACTCCTCGGA CGCGGAGGAG 
GAGGGCAGAG CGGGCCCCGG GAAGCTGGTC CCCGGGAGGT ACACCGTCAC CAGGCTGGAC 
GAGAAGGGGG TCTCCGACGT GCTGGCCCTG AGAAGCGGGG ACGAGGTGGA GCTCGTGCAG 
GAGGGAGATG AGGGCCTCTG GTGCGTGCGG AACCTGAGCT CCGGCGTCGA GGGCTGGGTG 
CCCGCCCGCA GCCTGTCCAC TCTCCTCTGC CAGGGTGGCC CCGTGGGGTG CCTGAGCAGC 
CCAGAGTCCA GTGCGGGTTC CGCGGTGCTG AGCCCCTCGT CCAGCTGCAG TGAGAGCTGC 
ACGGCCGCCC TCGCTGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_015473940.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329426.1 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA.

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CloneID	OBa92205
Clone ID Related Accession (Same CDS sequence)	XM_015465840.1 , XM_015473940.1
Accession Version	XM_015473940.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCCG CGACCTCGGG CTGCTTGCCT TCCTGGCTGA GGTGCATCAG CAGGCCGGCC 
GGCCGGCTGC AGGCCTGCCC AGAGGCCGGG AAGTGGGACA CGGACCTGTC AGTCAGCGTG 
CGCCTGCTCT TAGGCGCATT AGCAAAATCT CTGCAGGAAA CAGCGGGTGC CGAGCCAGAG 
CTCGTGCTCG GCTTAACTCT TCAACACCCG GGAGCCCGGG AAGCAGGTAG GGCCTCAGCC 
ACGGGTCAGG ATCCGCGGGG GACGTGTCCA CCGTCCCCCG GACAGTGGGG CGGGGACACA 
GTCTCTGTGC CCGCCTGGCT TCCTCCTGCC GGGCCTGCCT TTCCTCGGGA GCCCAGTTTC 
CTGACGTCCT CTCCGCTGGA TGTCGCCACA GGAGCCTGTC AGGATGAGAT CATGCACCAC 
GACATCAGCC CGCTCTGTGC CGCCGACATC CGGGACCAGC TGCAGAAGCG CTTCGCTTAC 
CTGTCAGGCG GGCGGGGCCA GGACGGCAGC CCGGTCATCA CCTTTCCCGA CTACCCGGCC 
TTCGGAGACG TCCCAGACCA GGACTTCCAG AATGTCATGA CCTACCTCAC CAGCATCCCC 
AGCCTGCAGG ACGCTGGCAT CGGGTTCATC CTGGTCATAG ACCGCAGGCA GGACAGATGG 
ACCTCGGTCA AGGCGTCCAT CCTGAGAATA GCGGCATCCT TCCCCGCAAA CCTGCAGCTC 
GTCCTCGTCC TGCGGCCAAC CGGGTTCTTC CAGAGGACAC TCTCCGACCT CGCCTTCAGG 
TTCAACAGAG ATGACTTTAA GATGAAGGTG CCGGTCATAA TGCTGAGCTC AGTCCCAGAA 
CTACACGGCT ACATCGACAA GTCCCAGCTG ACGGAGGACC TGGGTGGGAC GCTGGCCTAC 
TGCCACTCCA GGTGGCTCCG CCATCGCACA GCCATCGAGA GCTTCGCCCT CCTGGTCAAG 
CAGACGGCGC AGATGCTGCA GACCTTTGGG ACCGAGCTGG CCGAGACGGA GCTGCCCAAT 
GACGTGCAGT CCACGAGCTC CGTGCTCCTC GCCCACACGG AGAAGAAGGA CAGAGCCAAG 
GAGGATATGA GGCTGGCGCT TGTCGAGGGG CGGCGCGTCC TGGAGAGCAT CCACGAGCCA 
CTGGCCCGGA GCCCAGATCA GAGCCCCAAC CAGGACCAAC TGGACAGTCA GAGCACCGTG 
CACAGGCTCC TGGCCCAGCT GAACGAGACC GAGGCCGCCT TTGACGAGTT CTGGGCAAAA 
CATCAGCAAA AACTGGAGCA GTGCCTGCAG CTCCGGCATT TCGAGCAGGA CTTCCGAGAG 
GTCAAATCGG CCCTGGACGT GCTGGCCCAG AAGGTCACGA CTTTCACCGA CGTGGGCAAC 
AGCCTGGCAC ACGTGCAGCA CCTCCTGAAG GACCTGACCA GCTTCGAGGA GAAGTCCAGT 
GCCGCCGTGC AGCGGGCCCG CACGCTGTCC CAGGAGGGTG AGCGGCTCAT CGAGCACAAG 
CACTATGCCG TGGACTCCAT CCGCCCCAAG TGTCACGAGC TCCGCCACCT GTGCGACCAG 
TTCGCGGCCG ACGTGGAGCG GAGGAGGGGG CTGCTGGGCA AGTCGCTGGA GCTGCACGGC 
CTCCTGGAGG CGTCCATGAA GTGGTGTGAC GAGGGCATCT ACCTGCTGGC ATCGCAGCCT 
GTGGACAAGT GCCAGTCCCA GGATGGCGCA GAGGCCGCCC TCCAGGAGCT CGAGAGGTTT 
CTGGAGACGG GGGCAGAAAA TAAGATCCAG GAGCTCAGCA AGATCTTCCA GGACTACGAG 
CCCATCCTCA CCAAAGACCT GCTGGAGCAT GTGCAGAGGG TCTTCCAGAA GCAGGAGAGC 
GTGGAGGAGA TGTTCCACCG CAGGCAGGCC AGCCTCAAGA AGCTGGCGGC CAAGCAGACT 
CGGCCCGTGC AGCCCGTGGC CCCCCGGCCG GAAGCACTCA CCAAGTCGCC CTGCCCCTCC 
CCAGGCATCC GGAGGGGTTC TGAGAACCAC AGTTCCGAGG GCAGTGCACT CCGGAGGGGG 
CCCTACAGGA GGGCCAAGAG TGAGGTGAGC GAGGGCCGGC AGGGCCGTGG CGGCTCCACG 
GGGGACGAGG AGAGCCTGGC CGTCCTGCGG AGGCATGTGA TGAAGGAGCT TCTGGACACA 
GAGCGGGTCT ACGTGGAGGA GCTGCTGTGC GTCCTGGAGG GCTACGCCGC GGAGATGGAC 
AACCCGTTAA TGACTCATCT CATCTCCACT GGCCTTCAGA ACAAGAAGGA CATCCTGTTT 
GGAAACATGG AAGAAATTTA CCACTTTCAT AACAGGATAT TCCTGAGGGA GCTGGAGAAC 
TACACGGACT GCCCGGAGCT GGTTGGCAGG TGCTTTCTGG AGCGGATGGA GGAGTTCCAG 
ATCTACGAGA AGTACTGTCA GAACAAGCCG CGCTCCGAGA GCCTGTGGAG ACAGTGCTCC 
GACTGCCCCT TCTTCCAGGA GTGCCAGAAG AAGCTGGACC ACAAGCTGAG CCTGGACTCC 
TACCTGCTGA AGCCAGTCCA GAGGATCACC AAGTACCAGC TGCTGCTGAA GGAGATGCTT 
AAATACAGCA AGAGTTGCGA GGGGGCGGAG GACCTGCAGG AGGCCCTGAG CTCCATCCTG 
GGCATCCTCA AGGCCGTGAA CGACTCCATG CACCTGATCG CCATCACCGG CTACGAGGGG 
AACCTGAGTG ACCTGGGGAG GCTGCTCATG CAGGGCTCCT TCAGTGTGTG GACCGACCAC 
AAGAGGGGCC ACGCCAAGGT GAAGGACCTG GCCCGCTTCA AGCCCATGCA GCGCCACCTG 
TTCCTGCACG AGAAGGCCGT GCTCTTCTGT AAGCGGCGGG AGGAGAACGG CGAGGGCTAC 
GAGAAGGCGC CCTCCTACAG CTACAAGCAG TCCCTGAACA TGACGGCCGT CGGCATCACG 
GAGAACGTGA AAGGAGACGC CAAGAAGTTT GAGATCTGGT ACAACGCCCG GGAGGAGGTG 
TACATCATCC AGGCACCCAC TCCTGAAATC AAGGCCGCCT GGGTGAGCGA GATCCGGAAG 
GTGCTGACCA GCCAGCTGCA GGCATGCAGA GAGGCCAGCC AGCATCGCGC CCTCGAGCAG 
TCCCAGAGCC TGCCCCTGCC CACACCAGCT GGCACCAGTC CCTCGAAAGC GAGCACAAGA 
AACATAAAAA AGTTAGAAGA AAGAAAAACG GACCCGCTGA GCCTGGAGGG ATACGGGGGG 
CCGACACCAC AGCAGAGGGC CCCCGAGAAG GGCAAAGATG ACGCGGTCAC TAGCTCTACC 
TCAGAAAGCT CTGCCCTTTC TAAAAAGCGC TTCACCCTGC AGAGCTTTGC TGCCCTCAAA 
GCCCAGAAAG CTTCTCCCAC CAGTCCTGAC AAAAAAGCTA AGCGACACCA AGTAAAGAGC 
GACCCCACGC CTTTTGGGTT ACGAGGCTGG AGCAAAACGT CCCACCCATC AGAGACGCCT 
GAGGACAACG ATGGCTGGTC CAGCACCGAG GAGCCCGTCA ACTCCTCGGA CGCGGAGGAG 
GAGGGCAGAG CGGGCCCCGG GAAGCTGGTC CCCGGGAGGT ACACCGTCAC CAGGCTGGAC 
GAGAAGGGGG TCTCCGACGT GCTGGCCCTG AGAAGCGGGG ACGAGGTGGA GCTCGTGCAG 
GAGGGAGATG AGGGCCTCTG GTGCGTGCGG AACCTGAGCT CCGGCGTCGA GGGCTGGGTG 
CCCGCCCGCA GCCTGTCCAC TCTCCTCTGC CAGGGTGGCC CCGTGGGGTG CCTGAGCAGC 
CCAGAGTCCA GTGCGGGTTC CGCGGTGCTG AGCCCCTCGT CCAGCTGCAG TGAGAGCTGC 
ACGGCCGCCC TCGCTGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329426.1
CDS	120..3989
Translation	MLAATSGCLPSWLRCISRPAGRLQACPEAGKWDTDLSVSVRLLLGALAKSLQETAGAEPELVLGLTLQHPGAREAGRASATGQDPRGTCPPSPGQWGGDTVSVPAWLPPAGPAFPREPSFLTSSPLDVATGACQDEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKDDAVTSSTSESSALSKKRFTLQSFAALKAQKASPTSPDKKAKRHQVKSDPTPFGLRGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPARSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_015473940.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473940.1

ORF Insert Sequence:

ATGCTGGCCG CGACCTCGGG CTGCTTGCCT TCCTGGCTGA GGTGCATCAG CAGGCCGGCC 
GGCCGGCTGC AGGCCTGCCC AGAGGCCGGG AAGTGGGACA CGGACCTGTC AGTCAGCGTG 
CGCCTGCTCT TAGGCGCATT AGCAAAATCT CTGCAGGAAA CAGCGGGTGC CGAGCCAGAG 
CTCGTGCTCG GCTTAACTCT TCAACACCCG GGAGCCCGGG AAGCAGGTAG GGCCTCAGCC 
ACGGGTCAGG ATCCGCGGGG GACGTGTCCA CCGTCCCCCG GACAGTGGGG CGGGGACACA 
GTCTCTGTGC CCGCCTGGCT TCCTCCTGCC GGGCCTGCCT TTCCTCGGGA GCCCAGTTTC 
CTGACGTCCT CTCCGCTGGA TGTCGCCACA GGAGCCTGTC AGGATGAGAT CATGCACCAC 
GACATCAGCC CGCTCTGTGC CGCCGACATC CGGGACCAGC TGCAGAAGCG CTTCGCTTAC 
CTGTCAGGCG GGCGGGGCCA GGACGGCAGC CCGGTCATCA CCTTTCCCGA CTACCCGGCC 
TTCGGAGACG TCCCAGACCA GGACTTCCAG AATGTCATGA CCTACCTCAC CAGCATCCCC 
AGCCTGCAGG ACGCTGGCAT CGGGTTCATC CTGGTCATAG ACCGCAGGCA GGACAGATGG 
ACCTCGGTCA AGGCGTCCAT CCTGAGAATA GCGGCATCCT TCCCCGCAAA CCTGCAGCTC 
GTCCTCGTCC TGCGGCCAAC CGGGTTCTTC CAGAGGACAC TCTCCGACCT CGCCTTCAGG 
TTCAACAGAG ATGACTTTAA GATGAAGGTG CCGGTCATAA TGCTGAGCTC AGTCCCAGAA 
CTACACGGCT ACATCGACAA GTCCCAGCTG ACGGAGGACC TGGGTGGGAC GCTGGCCTAC 
TGCCACTCCA GGTGGCTCCG CCATCGCACA GCCATCGAGA GCTTCGCCCT CCTGGTCAAG 
CAGACGGCGC AGATGCTGCA GACCTTTGGG ACCGAGCTGG CCGAGACGGA GCTGCCCAAT 
GACGTGCAGT CCACGAGCTC CGTGCTCCTC GCCCACACGG AGAAGAAGGA CAGAGCCAAG 
GAGGATATGA GGCTGGCGCT TGTCGAGGGG CGGCGCGTCC TGGAGAGCAT CCACGAGCCA 
CTGGCCCGGA GCCCAGATCA GAGCCCCAAC CAGGACCAAC TGGACAGTCA GAGCACCGTG 
CACAGGCTCC TGGCCCAGCT GAACGAGACC GAGGCCGCCT TTGACGAGTT CTGGGCAAAA 
CATCAGCAAA AACTGGAGCA GTGCCTGCAG CTCCGGCATT TCGAGCAGGA CTTCCGAGAG 
GTCAAATCGG CCCTGGACGT GCTGGCCCAG AAGGTCACGA CTTTCACCGA CGTGGGCAAC 
AGCCTGGCAC ACGTGCAGCA CCTCCTGAAG GACCTGACCA GCTTCGAGGA GAAGTCCAGT 
GCCGCCGTGC AGCGGGCCCG CACGCTGTCC CAGGAGGGTG AGCGGCTCAT CGAGCACAAG 
CACTATGCCG TGGACTCCAT CCGCCCCAAG TGTCACGAGC TCCGCCACCT GTGCGACCAG 
TTCGCGGCCG ACGTGGAGCG GAGGAGGGGG CTGCTGGGCA AGTCGCTGGA GCTGCACGGC 
CTCCTGGAGG CGTCCATGAA GTGGTGTGAC GAGGGCATCT ACCTGCTGGC ATCGCAGCCT 
GTGGACAAGT GCCAGTCCCA GGATGGCGCA GAGGCCGCCC TCCAGGAGCT CGAGAGGTTT 
CTGGAGACGG GGGCAGAAAA TAAGATCCAG GAGCTCAGCA AGATCTTCCA GGACTACGAG 
CCCATCCTCA CCAAAGACCT GCTGGAGCAT GTGCAGAGGG TCTTCCAGAA GCAGGAGAGC 
GTGGAGGAGA TGTTCCACCG CAGGCAGGCC AGCCTCAAGA AGCTGGCGGC CAAGCAGACT 
CGGCCCGTGC AGCCCGTGGC CCCCCGGCCG GAAGCACTCA CCAAGTCGCC CTGCCCCTCC 
CCAGGCATCC GGAGGGGTTC TGAGAACCAC AGTTCCGAGG GCAGTGCACT CCGGAGGGGG 
CCCTACAGGA GGGCCAAGAG TGAGGTGAGC GAGGGCCGGC AGGGCCGTGG CGGCTCCACG 
GGGGACGAGG AGAGCCTGGC CGTCCTGCGG AGGCATGTGA TGAAGGAGCT TCTGGACACA 
GAGCGGGTCT ACGTGGAGGA GCTGCTGTGC GTCCTGGAGG GCTACGCCGC GGAGATGGAC 
AACCCGTTAA TGACTCATCT CATCTCCACT GGCCTTCAGA ACAAGAAGGA CATCCTGTTT 
GGAAACATGG AAGAAATTTA CCACTTTCAT AACAGGATAT TCCTGAGGGA GCTGGAGAAC 
TACACGGACT GCCCGGAGCT GGTTGGCAGG TGCTTTCTGG AGCGGATGGA GGAGTTCCAG 
ATCTACGAGA AGTACTGTCA GAACAAGCCG CGCTCCGAGA GCCTGTGGAG ACAGTGCTCC 
GACTGCCCCT TCTTCCAGGA GTGCCAGAAG AAGCTGGACC ACAAGCTGAG CCTGGACTCC 
TACCTGCTGA AGCCAGTCCA GAGGATCACC AAGTACCAGC TGCTGCTGAA GGAGATGCTT 
AAATACAGCA AGAGTTGCGA GGGGGCGGAG GACCTGCAGG AGGCCCTGAG CTCCATCCTG 
GGCATCCTCA AGGCCGTGAA CGACTCCATG CACCTGATCG CCATCACCGG CTACGAGGGG 
AACCTGAGTG ACCTGGGGAG GCTGCTCATG CAGGGCTCCT TCAGTGTGTG GACCGACCAC 
AAGAGGGGCC ACGCCAAGGT GAAGGACCTG GCCCGCTTCA AGCCCATGCA GCGCCACCTG 
TTCCTGCACG AGAAGGCCGT GCTCTTCTGT AAGCGGCGGG AGGAGAACGG CGAGGGCTAC 
GAGAAGGCGC CCTCCTACAG CTACAAGCAG TCCCTGAACA TGACGGCCGT CGGCATCACG 
GAGAACGTGA AAGGAGACGC CAAGAAGTTT GAGATCTGGT ACAACGCCCG GGAGGAGGTG 
TACATCATCC AGGCACCCAC TCCTGAAATC AAGGCCGCCT GGGTGAGCGA GATCCGGAAG 
GTGCTGACCA GCCAGCTGCA GGCATGCAGA GAGGCCAGCC AGCATCGCGC CCTCGAGCAG 
TCCCAGAGCC TGCCCCTGCC CACACCAGCT GGCACCAGTC CCTCGAAAGC GAGCACAAGA 
AACATAAAAA AGTTAGAAGA AAGAAAAACG GACCCGCTGA GCCTGGAGGG ATACGGGGGG 
CCGACACCAC AGCAGAGGGC CCCCGAGAAG GGCAAAGATG ACGCGGTCAC TAGCTCTACC 
TCAGAAAGCT CTGCCCTTTC TAAAAAGCGC TTCACCCTGC AGAGCTTTGC TGCCCTCAAA 
GCCCAGAAAG CTTCTCCCAC CAGTCCTGAC AAAAAAGCTA AGCGACACCA AGTAAAGAGC 
GACCCCACGC CTTTTGGGTT ACGAGGCTGG AGCAAAACGT CCCACCCATC AGAGACGCCT 
GAGGACAACG ATGGCTGGTC CAGCACCGAG GAGCCCGTCA ACTCCTCGGA CGCGGAGGAG 
GAGGGCAGAG CGGGCCCCGG GAAGCTGGTC CCCGGGAGGT ACACCGTCAC CAGGCTGGAC 
GAGAAGGGGG TCTCCGACGT GCTGGCCCTG AGAAGCGGGG ACGAGGTGGA GCTCGTGCAG 
GAGGGAGATG AGGGCCTCTG GTGCGTGCGG AACCTGAGCT CCGGCGTCGA GGGCTGGGTG 
CCCGCCCGCA GCCTGTCCAC TCTCCTCTGC CAGGGTGGCC CCGTGGGGTG CCTGAGCAGC 
CCAGAGTCCA GTGCGGGTTC CGCGGTGCTG AGCCCCTCGT CCAGCTGCAG TGAGAGCTGC 
ACGGCCGCCC TCGCTGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_015465841.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321327.1 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa92206
Clone ID Related Accession (Same CDS sequence)	XM_015465841.1 , XM_015473941.1
Accession Version	XM_015465841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646112.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCCG CGACCTCGGG CTGCTTGCCT TCCTGGCTGA GGTGCATCAG CAGGCCGGCC 
GGCCGGCTGC AGGCCTGCCC AGAGGCCGGG AAGTGGGACA CGGACCTGTC AGTCAGCGTG 
CGCCTGCTCT TAGGCGCATT AGCAAAATCT CTGCAGGAAA CAGCGGGTGC CGAGCCAGAG 
CTCGTGCTCG GCTTAACTCT TCAACACCCG GGAGCCCGGG AAGCAGGTAG GGCCTCAGCC 
ACGGGTCAGG ATCCGCGGGG GACGTGTCCA CCGTCCCCCG GACAGTGGGG CGGGGACACA 
GTCTCTGTGC CCGCCTGGCT TCCTCCTGCC GGGCCTGCCT TTCCTCGGGA GCCCAGTTTC 
CTGACGTCCT CTCCGCTGGA TGTCGCCACA GGAGCCTGTC AGGATGAGAT CATGCACCAC 
GACATCAGCC CGCTCTGTGC CGCCGACATC CGGGACCAGC TGCAGAAGCG CTTCGCTTAC 
CTGTCAGGCG GGCGGGGCCA GGACGGCAGC CCGGTCATCA CCTTTCCCGA CTACCCGGCC 
TTCGGAGACG TCCCAGACCA GGACTTCCAG AATGTCATGA CCTACCTCAC CAGCATCCCC 
AGCCTGCAGG ACGCTGGCAT CGGGTTCATC CTGGTCATAG ACCGCAGGCA GGACAGATGG 
ACCTCGGTCA AGGCGTCCAT CCTGAGAATA GCGGCATCCT TCCCCGCAAA CCTGCAGCTC 
GTCCTCGTCC TGCGGCCAAC CGGGTTCTTC CAGAGGACAC TCTCCGACCT CGCCTTCAGG 
TTCAACAGAG ATGACTTTAA GATGAAGGTG CCGGTCATAA TGCTGAGCTC AGTCCCAGAA 
CTACACGGCT ACATCGACAA GTCCCAGCTG ACGGAGGACC TGGGTGGGAC GCTGGCCTAC 
TGCCACTCCA GGTGGCTCCG CCATCGCACA GCCATCGAGA GCTTCGCCCT CCTGGTCAAG 
CAGACGGCGC AGATGCTGCA GACCTTTGGG ACCGAGCTGG CCGAGACGGA GCTGCCCAAT 
GACGTGCAGT CCACGAGCTC CGTGCTCCTC GCCCACACGG AGAAGAAGGA CAGAGCCAAG 
GAGGATATGA GGCTGGCGCT TGTCGAGGGG CGGCGCGTCC TGGAGAGCAT CCACGAGCCA 
CTGGCCCGGA GCCCAGATCA GAGCCCCAAC CAGGACCAAC TGGACAGTCA GAGCACCGTG 
CACAGGCTCC TGGCCCAGCT GAACGAGACC GAGGCCGCCT TTGACGAGTT CTGGGCAAAA 
CATCAGCAAA AACTGGAGCA GTGCCTGCAG CTCCGGCATT TCGAGCAGGA CTTCCGAGAG 
GTCAAATCGG CCCTGGACGT GCTGGCCCAG AAGGTCACGA CTTTCACCGA CGTGGGCAAC 
AGCCTGGCAC ACGTGCAGCA CCTCCTGAAG GACCTGACCA GCTTCGAGGA GAAGTCCAGT 
GCCGCCGTGC AGCGGGCCCG CACGCTGTCC CAGGAGGGTG AGCGGCTCAT CGAGCACAAG 
CACTATGCCG TGGACTCCAT CCGCCCCAAG TGTCACGAGC TCCGCCACCT GTGCGACCAG 
TTCGCGGCCG ACGTGGAGCG GAGGAGGGGG CTGCTGGGCA AGTCGCTGGA GCTGCACGGC 
CTCCTGGAGG CGTCCATGAA GTGGTGTGAC GAGGGCATCT ACCTGCTGGC ATCGCAGCCT 
GTGGACAAGT GCCAGTCCCA GGATGGCGCA GAGGCCGCCC TCCAGGAGCT CGAGAGGTTT 
CTGGAGACGG GGGCAGAAAA TAAGATCCAG GAGCTCAGCA AGATCTTCCA GGACTACGAG 
CCCATCCTCA CCAAAGACCT GCTGGAGCAT GTGCAGAGGG TCTTCCAGAA GCAGGAGAGC 
GTGGAGGAGA TGTTCCACCG CAGGCAGGCC AGCCTCAAGA AGCTGGCGGC CAAGCAGACT 
CGGCCCGTGC AGCCCGTGGC CCCCCGGCCG GAAGCACTCA CCAAGTCGCC CTGCCCCTCC 
CCAGGCATCC GGAGGGGTTC TGAGAACCAC AGTTCCGAGG GCAGTGCACT CCGGAGGGGG 
CCCTACAGGA GGGCCAAGAG TGAGGTGAGC GAGGGCCGGC AGGGCCGTGG CGGCTCCACG 
GGGGACGAGG AGAGCCTGGC CGTCCTGCGG AGGCATGTGA TGAAGGAGCT TCTGGACACA 
GAGCGGGTCT ACGTGGAGGA GCTGCTGTGC GTCCTGGAGG GCTACGCCGC GGAGATGGAC 
AACCCGTTAA TGACTCATCT CATCTCCACT GGCCTTCAGA ACAAGAAGGA CATCCTGTTT 
GGAAACATGG AAGAAATTTA CCACTTTCAT AACAGGATAT TCCTGAGGGA GCTGGAGAAC 
TACACGGACT GCCCGGAGCT GGTTGGCAGG TGCTTTCTGG AGCGGATGGA GGAGTTCCAG 
ATCTACGAGA AGTACTGTCA GAACAAGCCG CGCTCCGAGA GCCTGTGGAG ACAGTGCTCC 
GACTGCCCCT TCTTCCAGGA GTGCCAGAAG AAGCTGGACC ACAAGCTGAG CCTGGACTCC 
TACCTGCTGA AGCCAGTCCA GAGGATCACC AAGTACCAGC TGCTGCTGAA GGAGATGCTT 
AAATACAGCA AGAGTTGCGA GGGGGCGGAG GACCTGCAGG AGGCCCTGAG CTCCATCCTG 
GGCATCCTCA AGGCCGTGAA CGACTCCATG CACCTGATCG CCATCACCGG CTACGAGGGG 
AACCTGAGTG ACCTGGGGAG GCTGCTCATG CAGGGCTCCT TCAGTGTGTG GACCGACCAC 
AAGAGGGGCC ACGCCAAGGT GAAGGACCTG GCCCGCTTCA AGCCCATGCA GCGCCACCTG 
TTCCTGCACG AGAAGGCCGT GCTCTTCTGT AAGCGGCGGG AGGAGAACGG CGAGGGCTAC 
GAGAAGGCGC CCTCCTACAG CTACAAGCAG TCCCTGAACA TGACGGCCGT CGGCATCACG 
GAGAACGTGA AAGGAGACGC CAAGAAGTTT GAGATCTGGT ACAACGCCCG GGAGGAGGTG 
TACATCATCC AGGCACCCAC TCCTGAAATC AAGGCCGCCT GGGTGAGCGA GATCCGGAAG 
GTGCTGACCA GCCAGCTGCA GGCATGCAGA GAGGCCAGCC AGCATCGCGC CCTCGAGCAG 
TCCCAGAGCC TGCCCCTGCC CACACCAGCT GGCACCAGTC CCTCGAAAGC GAGCACAAGA 
AACATAAAAA AGTTAGAAGA AAGAAAAACG GACCCGCTGA GCCTGGAGGG ATACGGGGGG 
CCGACACCAC AGCAGAGGGC CCCCGAGAAG GGCAAAGGCT GGAGCAAAAC GTCCCACCCA 
TCAGAGACGC CTGAGGACAA CGATGGCTGG TCCAGCACCG AGGAGCCCGT CAACTCCTCG 
GACGCGGAGG AGGAGGGCAG AGCGGGCCCC GGGAAGCTGG TCCCCGGGAG GTACACCGTC 
ACCAGGCTGG ACGAGAAGGG GGTCTCCGAC GTGCTGGCCC TGAGAAGCGG GGACGAGGTG 
GAGCTCGTGC AGGAGGGAGA TGAGGGCCTC TGGTGCGTGC GGAACCTGAG CTCCGGCGTC 
GAGGGCTGGG TGCCCGCCCG CAGCCTGTCC ACTCTCCTCT GCCAGGGTGG CCCCGTGGGG 
TGCCTGAGCA GCCCAGAGTC CAGTGCGGGT TCCGCGGTGC TGAGCCCCTC GTCCAGCTGC 
AGTGAGAGCT GCACGGCCGC CCTCGCTGAC CTGCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321327.1
CDS	120..3821
Translation	MLAATSGCLPSWLRCISRPAGRLQACPEAGKWDTDLSVSVRLLLGALAKSLQETAGAEPELVLGLTLQHPGAREAGRASATGQDPRGTCPPSPGQWGGDTVSVPAWLPPAGPAFPREPSFLTSSPLDVATGACQDEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPARSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_015465841.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465841.1

ORF Insert Sequence:

ATGCTGGCCG CGACCTCGGG CTGCTTGCCT TCCTGGCTGA GGTGCATCAG CAGGCCGGCC 
GGCCGGCTGC AGGCCTGCCC AGAGGCCGGG AAGTGGGACA CGGACCTGTC AGTCAGCGTG 
CGCCTGCTCT TAGGCGCATT AGCAAAATCT CTGCAGGAAA CAGCGGGTGC CGAGCCAGAG 
CTCGTGCTCG GCTTAACTCT TCAACACCCG GGAGCCCGGG AAGCAGGTAG GGCCTCAGCC 
ACGGGTCAGG ATCCGCGGGG GACGTGTCCA CCGTCCCCCG GACAGTGGGG CGGGGACACA 
GTCTCTGTGC CCGCCTGGCT TCCTCCTGCC GGGCCTGCCT TTCCTCGGGA GCCCAGTTTC 
CTGACGTCCT CTCCGCTGGA TGTCGCCACA GGAGCCTGTC AGGATGAGAT CATGCACCAC 
GACATCAGCC CGCTCTGTGC CGCCGACATC CGGGACCAGC TGCAGAAGCG CTTCGCTTAC 
CTGTCAGGCG GGCGGGGCCA GGACGGCAGC CCGGTCATCA CCTTTCCCGA CTACCCGGCC 
TTCGGAGACG TCCCAGACCA GGACTTCCAG AATGTCATGA CCTACCTCAC CAGCATCCCC 
AGCCTGCAGG ACGCTGGCAT CGGGTTCATC CTGGTCATAG ACCGCAGGCA GGACAGATGG 
ACCTCGGTCA AGGCGTCCAT CCTGAGAATA GCGGCATCCT TCCCCGCAAA CCTGCAGCTC 
GTCCTCGTCC TGCGGCCAAC CGGGTTCTTC CAGAGGACAC TCTCCGACCT CGCCTTCAGG 
TTCAACAGAG ATGACTTTAA GATGAAGGTG CCGGTCATAA TGCTGAGCTC AGTCCCAGAA 
CTACACGGCT ACATCGACAA GTCCCAGCTG ACGGAGGACC TGGGTGGGAC GCTGGCCTAC 
TGCCACTCCA GGTGGCTCCG CCATCGCACA GCCATCGAGA GCTTCGCCCT CCTGGTCAAG 
CAGACGGCGC AGATGCTGCA GACCTTTGGG ACCGAGCTGG CCGAGACGGA GCTGCCCAAT 
GACGTGCAGT CCACGAGCTC CGTGCTCCTC GCCCACACGG AGAAGAAGGA CAGAGCCAAG 
GAGGATATGA GGCTGGCGCT TGTCGAGGGG CGGCGCGTCC TGGAGAGCAT CCACGAGCCA 
CTGGCCCGGA GCCCAGATCA GAGCCCCAAC CAGGACCAAC TGGACAGTCA GAGCACCGTG 
CACAGGCTCC TGGCCCAGCT GAACGAGACC GAGGCCGCCT TTGACGAGTT CTGGGCAAAA 
CATCAGCAAA AACTGGAGCA GTGCCTGCAG CTCCGGCATT TCGAGCAGGA CTTCCGAGAG 
GTCAAATCGG CCCTGGACGT GCTGGCCCAG AAGGTCACGA CTTTCACCGA CGTGGGCAAC 
AGCCTGGCAC ACGTGCAGCA CCTCCTGAAG GACCTGACCA GCTTCGAGGA GAAGTCCAGT 
GCCGCCGTGC AGCGGGCCCG CACGCTGTCC CAGGAGGGTG AGCGGCTCAT CGAGCACAAG 
CACTATGCCG TGGACTCCAT CCGCCCCAAG TGTCACGAGC TCCGCCACCT GTGCGACCAG 
TTCGCGGCCG ACGTGGAGCG GAGGAGGGGG CTGCTGGGCA AGTCGCTGGA GCTGCACGGC 
CTCCTGGAGG CGTCCATGAA GTGGTGTGAC GAGGGCATCT ACCTGCTGGC ATCGCAGCCT 
GTGGACAAGT GCCAGTCCCA GGATGGCGCA GAGGCCGCCC TCCAGGAGCT CGAGAGGTTT 
CTGGAGACGG GGGCAGAAAA TAAGATCCAG GAGCTCAGCA AGATCTTCCA GGACTACGAG 
CCCATCCTCA CCAAAGACCT GCTGGAGCAT GTGCAGAGGG TCTTCCAGAA GCAGGAGAGC 
GTGGAGGAGA TGTTCCACCG CAGGCAGGCC AGCCTCAAGA AGCTGGCGGC CAAGCAGACT 
CGGCCCGTGC AGCCCGTGGC CCCCCGGCCG GAAGCACTCA CCAAGTCGCC CTGCCCCTCC 
CCAGGCATCC GGAGGGGTTC TGAGAACCAC AGTTCCGAGG GCAGTGCACT CCGGAGGGGG 
CCCTACAGGA GGGCCAAGAG TGAGGTGAGC GAGGGCCGGC AGGGCCGTGG CGGCTCCACG 
GGGGACGAGG AGAGCCTGGC CGTCCTGCGG AGGCATGTGA TGAAGGAGCT TCTGGACACA 
GAGCGGGTCT ACGTGGAGGA GCTGCTGTGC GTCCTGGAGG GCTACGCCGC GGAGATGGAC 
AACCCGTTAA TGACTCATCT CATCTCCACT GGCCTTCAGA ACAAGAAGGA CATCCTGTTT 
GGAAACATGG AAGAAATTTA CCACTTTCAT AACAGGATAT TCCTGAGGGA GCTGGAGAAC 
TACACGGACT GCCCGGAGCT GGTTGGCAGG TGCTTTCTGG AGCGGATGGA GGAGTTCCAG 
ATCTACGAGA AGTACTGTCA GAACAAGCCG CGCTCCGAGA GCCTGTGGAG ACAGTGCTCC 
GACTGCCCCT TCTTCCAGGA GTGCCAGAAG AAGCTGGACC ACAAGCTGAG CCTGGACTCC 
TACCTGCTGA AGCCAGTCCA GAGGATCACC AAGTACCAGC TGCTGCTGAA GGAGATGCTT 
AAATACAGCA AGAGTTGCGA GGGGGCGGAG GACCTGCAGG AGGCCCTGAG CTCCATCCTG 
GGCATCCTCA AGGCCGTGAA CGACTCCATG CACCTGATCG CCATCACCGG CTACGAGGGG 
AACCTGAGTG ACCTGGGGAG GCTGCTCATG CAGGGCTCCT TCAGTGTGTG GACCGACCAC 
AAGAGGGGCC ACGCCAAGGT GAAGGACCTG GCCCGCTTCA AGCCCATGCA GCGCCACCTG 
TTCCTGCACG AGAAGGCCGT GCTCTTCTGT AAGCGGCGGG AGGAGAACGG CGAGGGCTAC 
GAGAAGGCGC CCTCCTACAG CTACAAGCAG TCCCTGAACA TGACGGCCGT CGGCATCACG 
GAGAACGTGA AAGGAGACGC CAAGAAGTTT GAGATCTGGT ACAACGCCCG GGAGGAGGTG 
TACATCATCC AGGCACCCAC TCCTGAAATC AAGGCCGCCT GGGTGAGCGA GATCCGGAAG 
GTGCTGACCA GCCAGCTGCA GGCATGCAGA GAGGCCAGCC AGCATCGCGC CCTCGAGCAG 
TCCCAGAGCC TGCCCCTGCC CACACCAGCT GGCACCAGTC CCTCGAAAGC GAGCACAAGA 
AACATAAAAA AGTTAGAAGA AAGAAAAACG GACCCGCTGA GCCTGGAGGG ATACGGGGGG 
CCGACACCAC AGCAGAGGGC CCCCGAGAAG GGCAAAGGCT GGAGCAAAAC GTCCCACCCA 
TCAGAGACGC CTGAGGACAA CGATGGCTGG TCCAGCACCG AGGAGCCCGT CAACTCCTCG 
GACGCGGAGG AGGAGGGCAG AGCGGGCCCC GGGAAGCTGG TCCCCGGGAG GTACACCGTC 
ACCAGGCTGG ACGAGAAGGG GGTCTCCGAC GTGCTGGCCC TGAGAAGCGG GGACGAGGTG 
GAGCTCGTGC AGGAGGGAGA TGAGGGCCTC TGGTGCGTGC GGAACCTGAG CTCCGGCGTC 
GAGGGCTGGG TGCCCGCCCG CAGCCTGTCC ACTCTCCTCT GCCAGGGTGG CCCCGTGGGG 
TGCCTGAGCA GCCCAGAGTC CAGTGCGGGT TCCGCGGTGC TGAGCCCCTC GTCCAGCTGC 
AGTGAGAGCT GCACGGCCGC CCTCGCTGAC CTGCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_015473941.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329427.1 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa92206
Clone ID Related Accession (Same CDS sequence)	XM_015465841.1 , XM_015473941.1
Accession Version	XM_015473941.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCCG CGACCTCGGG CTGCTTGCCT TCCTGGCTGA GGTGCATCAG CAGGCCGGCC 
GGCCGGCTGC AGGCCTGCCC AGAGGCCGGG AAGTGGGACA CGGACCTGTC AGTCAGCGTG 
CGCCTGCTCT TAGGCGCATT AGCAAAATCT CTGCAGGAAA CAGCGGGTGC CGAGCCAGAG 
CTCGTGCTCG GCTTAACTCT TCAACACCCG GGAGCCCGGG AAGCAGGTAG GGCCTCAGCC 
ACGGGTCAGG ATCCGCGGGG GACGTGTCCA CCGTCCCCCG GACAGTGGGG CGGGGACACA 
GTCTCTGTGC CCGCCTGGCT TCCTCCTGCC GGGCCTGCCT TTCCTCGGGA GCCCAGTTTC 
CTGACGTCCT CTCCGCTGGA TGTCGCCACA GGAGCCTGTC AGGATGAGAT CATGCACCAC 
GACATCAGCC CGCTCTGTGC CGCCGACATC CGGGACCAGC TGCAGAAGCG CTTCGCTTAC 
CTGTCAGGCG GGCGGGGCCA GGACGGCAGC CCGGTCATCA CCTTTCCCGA CTACCCGGCC 
TTCGGAGACG TCCCAGACCA GGACTTCCAG AATGTCATGA CCTACCTCAC CAGCATCCCC 
AGCCTGCAGG ACGCTGGCAT CGGGTTCATC CTGGTCATAG ACCGCAGGCA GGACAGATGG 
ACCTCGGTCA AGGCGTCCAT CCTGAGAATA GCGGCATCCT TCCCCGCAAA CCTGCAGCTC 
GTCCTCGTCC TGCGGCCAAC CGGGTTCTTC CAGAGGACAC TCTCCGACCT CGCCTTCAGG 
TTCAACAGAG ATGACTTTAA GATGAAGGTG CCGGTCATAA TGCTGAGCTC AGTCCCAGAA 
CTACACGGCT ACATCGACAA GTCCCAGCTG ACGGAGGACC TGGGTGGGAC GCTGGCCTAC 
TGCCACTCCA GGTGGCTCCG CCATCGCACA GCCATCGAGA GCTTCGCCCT CCTGGTCAAG 
CAGACGGCGC AGATGCTGCA GACCTTTGGG ACCGAGCTGG CCGAGACGGA GCTGCCCAAT 
GACGTGCAGT CCACGAGCTC CGTGCTCCTC GCCCACACGG AGAAGAAGGA CAGAGCCAAG 
GAGGATATGA GGCTGGCGCT TGTCGAGGGG CGGCGCGTCC TGGAGAGCAT CCACGAGCCA 
CTGGCCCGGA GCCCAGATCA GAGCCCCAAC CAGGACCAAC TGGACAGTCA GAGCACCGTG 
CACAGGCTCC TGGCCCAGCT GAACGAGACC GAGGCCGCCT TTGACGAGTT CTGGGCAAAA 
CATCAGCAAA AACTGGAGCA GTGCCTGCAG CTCCGGCATT TCGAGCAGGA CTTCCGAGAG 
GTCAAATCGG CCCTGGACGT GCTGGCCCAG AAGGTCACGA CTTTCACCGA CGTGGGCAAC 
AGCCTGGCAC ACGTGCAGCA CCTCCTGAAG GACCTGACCA GCTTCGAGGA GAAGTCCAGT 
GCCGCCGTGC AGCGGGCCCG CACGCTGTCC CAGGAGGGTG AGCGGCTCAT CGAGCACAAG 
CACTATGCCG TGGACTCCAT CCGCCCCAAG TGTCACGAGC TCCGCCACCT GTGCGACCAG 
TTCGCGGCCG ACGTGGAGCG GAGGAGGGGG CTGCTGGGCA AGTCGCTGGA GCTGCACGGC 
CTCCTGGAGG CGTCCATGAA GTGGTGTGAC GAGGGCATCT ACCTGCTGGC ATCGCAGCCT 
GTGGACAAGT GCCAGTCCCA GGATGGCGCA GAGGCCGCCC TCCAGGAGCT CGAGAGGTTT 
CTGGAGACGG GGGCAGAAAA TAAGATCCAG GAGCTCAGCA AGATCTTCCA GGACTACGAG 
CCCATCCTCA CCAAAGACCT GCTGGAGCAT GTGCAGAGGG TCTTCCAGAA GCAGGAGAGC 
GTGGAGGAGA TGTTCCACCG CAGGCAGGCC AGCCTCAAGA AGCTGGCGGC CAAGCAGACT 
CGGCCCGTGC AGCCCGTGGC CCCCCGGCCG GAAGCACTCA CCAAGTCGCC CTGCCCCTCC 
CCAGGCATCC GGAGGGGTTC TGAGAACCAC AGTTCCGAGG GCAGTGCACT CCGGAGGGGG 
CCCTACAGGA GGGCCAAGAG TGAGGTGAGC GAGGGCCGGC AGGGCCGTGG CGGCTCCACG 
GGGGACGAGG AGAGCCTGGC CGTCCTGCGG AGGCATGTGA TGAAGGAGCT TCTGGACACA 
GAGCGGGTCT ACGTGGAGGA GCTGCTGTGC GTCCTGGAGG GCTACGCCGC GGAGATGGAC 
AACCCGTTAA TGACTCATCT CATCTCCACT GGCCTTCAGA ACAAGAAGGA CATCCTGTTT 
GGAAACATGG AAGAAATTTA CCACTTTCAT AACAGGATAT TCCTGAGGGA GCTGGAGAAC 
TACACGGACT GCCCGGAGCT GGTTGGCAGG TGCTTTCTGG AGCGGATGGA GGAGTTCCAG 
ATCTACGAGA AGTACTGTCA GAACAAGCCG CGCTCCGAGA GCCTGTGGAG ACAGTGCTCC 
GACTGCCCCT TCTTCCAGGA GTGCCAGAAG AAGCTGGACC ACAAGCTGAG CCTGGACTCC 
TACCTGCTGA AGCCAGTCCA GAGGATCACC AAGTACCAGC TGCTGCTGAA GGAGATGCTT 
AAATACAGCA AGAGTTGCGA GGGGGCGGAG GACCTGCAGG AGGCCCTGAG CTCCATCCTG 
GGCATCCTCA AGGCCGTGAA CGACTCCATG CACCTGATCG CCATCACCGG CTACGAGGGG 
AACCTGAGTG ACCTGGGGAG GCTGCTCATG CAGGGCTCCT TCAGTGTGTG GACCGACCAC 
AAGAGGGGCC ACGCCAAGGT GAAGGACCTG GCCCGCTTCA AGCCCATGCA GCGCCACCTG 
TTCCTGCACG AGAAGGCCGT GCTCTTCTGT AAGCGGCGGG AGGAGAACGG CGAGGGCTAC 
GAGAAGGCGC CCTCCTACAG CTACAAGCAG TCCCTGAACA TGACGGCCGT CGGCATCACG 
GAGAACGTGA AAGGAGACGC CAAGAAGTTT GAGATCTGGT ACAACGCCCG GGAGGAGGTG 
TACATCATCC AGGCACCCAC TCCTGAAATC AAGGCCGCCT GGGTGAGCGA GATCCGGAAG 
GTGCTGACCA GCCAGCTGCA GGCATGCAGA GAGGCCAGCC AGCATCGCGC CCTCGAGCAG 
TCCCAGAGCC TGCCCCTGCC CACACCAGCT GGCACCAGTC CCTCGAAAGC GAGCACAAGA 
AACATAAAAA AGTTAGAAGA AAGAAAAACG GACCCGCTGA GCCTGGAGGG ATACGGGGGG 
CCGACACCAC AGCAGAGGGC CCCCGAGAAG GGCAAAGGCT GGAGCAAAAC GTCCCACCCA 
TCAGAGACGC CTGAGGACAA CGATGGCTGG TCCAGCACCG AGGAGCCCGT CAACTCCTCG 
GACGCGGAGG AGGAGGGCAG AGCGGGCCCC GGGAAGCTGG TCCCCGGGAG GTACACCGTC 
ACCAGGCTGG ACGAGAAGGG GGTCTCCGAC GTGCTGGCCC TGAGAAGCGG GGACGAGGTG 
GAGCTCGTGC AGGAGGGAGA TGAGGGCCTC TGGTGCGTGC GGAACCTGAG CTCCGGCGTC 
GAGGGCTGGG TGCCCGCCCG CAGCCTGTCC ACTCTCCTCT GCCAGGGTGG CCCCGTGGGG 
TGCCTGAGCA GCCCAGAGTC CAGTGCGGGT TCCGCGGTGC TGAGCCCCTC GTCCAGCTGC 
AGTGAGAGCT GCACGGCCGC CCTCGCTGAC CTGCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329427.1
CDS	120..3821
Translation	MLAATSGCLPSWLRCISRPAGRLQACPEAGKWDTDLSVSVRLLLGALAKSLQETAGAEPELVLGLTLQHPGAREAGRASATGQDPRGTCPPSPGQWGGDTVSVPAWLPPAGPAFPREPSFLTSSPLDVATGACQDEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPARSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_015473941.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473941.1

ORF Insert Sequence:

ATGCTGGCCG CGACCTCGGG CTGCTTGCCT TCCTGGCTGA GGTGCATCAG CAGGCCGGCC 
GGCCGGCTGC AGGCCTGCCC AGAGGCCGGG AAGTGGGACA CGGACCTGTC AGTCAGCGTG 
CGCCTGCTCT TAGGCGCATT AGCAAAATCT CTGCAGGAAA CAGCGGGTGC CGAGCCAGAG 
CTCGTGCTCG GCTTAACTCT TCAACACCCG GGAGCCCGGG AAGCAGGTAG GGCCTCAGCC 
ACGGGTCAGG ATCCGCGGGG GACGTGTCCA CCGTCCCCCG GACAGTGGGG CGGGGACACA 
GTCTCTGTGC CCGCCTGGCT TCCTCCTGCC GGGCCTGCCT TTCCTCGGGA GCCCAGTTTC 
CTGACGTCCT CTCCGCTGGA TGTCGCCACA GGAGCCTGTC AGGATGAGAT CATGCACCAC 
GACATCAGCC CGCTCTGTGC CGCCGACATC CGGGACCAGC TGCAGAAGCG CTTCGCTTAC 
CTGTCAGGCG GGCGGGGCCA GGACGGCAGC CCGGTCATCA CCTTTCCCGA CTACCCGGCC 
TTCGGAGACG TCCCAGACCA GGACTTCCAG AATGTCATGA CCTACCTCAC CAGCATCCCC 
AGCCTGCAGG ACGCTGGCAT CGGGTTCATC CTGGTCATAG ACCGCAGGCA GGACAGATGG 
ACCTCGGTCA AGGCGTCCAT CCTGAGAATA GCGGCATCCT TCCCCGCAAA CCTGCAGCTC 
GTCCTCGTCC TGCGGCCAAC CGGGTTCTTC CAGAGGACAC TCTCCGACCT CGCCTTCAGG 
TTCAACAGAG ATGACTTTAA GATGAAGGTG CCGGTCATAA TGCTGAGCTC AGTCCCAGAA 
CTACACGGCT ACATCGACAA GTCCCAGCTG ACGGAGGACC TGGGTGGGAC GCTGGCCTAC 
TGCCACTCCA GGTGGCTCCG CCATCGCACA GCCATCGAGA GCTTCGCCCT CCTGGTCAAG 
CAGACGGCGC AGATGCTGCA GACCTTTGGG ACCGAGCTGG CCGAGACGGA GCTGCCCAAT 
GACGTGCAGT CCACGAGCTC CGTGCTCCTC GCCCACACGG AGAAGAAGGA CAGAGCCAAG 
GAGGATATGA GGCTGGCGCT TGTCGAGGGG CGGCGCGTCC TGGAGAGCAT CCACGAGCCA 
CTGGCCCGGA GCCCAGATCA GAGCCCCAAC CAGGACCAAC TGGACAGTCA GAGCACCGTG 
CACAGGCTCC TGGCCCAGCT GAACGAGACC GAGGCCGCCT TTGACGAGTT CTGGGCAAAA 
CATCAGCAAA AACTGGAGCA GTGCCTGCAG CTCCGGCATT TCGAGCAGGA CTTCCGAGAG 
GTCAAATCGG CCCTGGACGT GCTGGCCCAG AAGGTCACGA CTTTCACCGA CGTGGGCAAC 
AGCCTGGCAC ACGTGCAGCA CCTCCTGAAG GACCTGACCA GCTTCGAGGA GAAGTCCAGT 
GCCGCCGTGC AGCGGGCCCG CACGCTGTCC CAGGAGGGTG AGCGGCTCAT CGAGCACAAG 
CACTATGCCG TGGACTCCAT CCGCCCCAAG TGTCACGAGC TCCGCCACCT GTGCGACCAG 
TTCGCGGCCG ACGTGGAGCG GAGGAGGGGG CTGCTGGGCA AGTCGCTGGA GCTGCACGGC 
CTCCTGGAGG CGTCCATGAA GTGGTGTGAC GAGGGCATCT ACCTGCTGGC ATCGCAGCCT 
GTGGACAAGT GCCAGTCCCA GGATGGCGCA GAGGCCGCCC TCCAGGAGCT CGAGAGGTTT 
CTGGAGACGG GGGCAGAAAA TAAGATCCAG GAGCTCAGCA AGATCTTCCA GGACTACGAG 
CCCATCCTCA CCAAAGACCT GCTGGAGCAT GTGCAGAGGG TCTTCCAGAA GCAGGAGAGC 
GTGGAGGAGA TGTTCCACCG CAGGCAGGCC AGCCTCAAGA AGCTGGCGGC CAAGCAGACT 
CGGCCCGTGC AGCCCGTGGC CCCCCGGCCG GAAGCACTCA CCAAGTCGCC CTGCCCCTCC 
CCAGGCATCC GGAGGGGTTC TGAGAACCAC AGTTCCGAGG GCAGTGCACT CCGGAGGGGG 
CCCTACAGGA GGGCCAAGAG TGAGGTGAGC GAGGGCCGGC AGGGCCGTGG CGGCTCCACG 
GGGGACGAGG AGAGCCTGGC CGTCCTGCGG AGGCATGTGA TGAAGGAGCT TCTGGACACA 
GAGCGGGTCT ACGTGGAGGA GCTGCTGTGC GTCCTGGAGG GCTACGCCGC GGAGATGGAC 
AACCCGTTAA TGACTCATCT CATCTCCACT GGCCTTCAGA ACAAGAAGGA CATCCTGTTT 
GGAAACATGG AAGAAATTTA CCACTTTCAT AACAGGATAT TCCTGAGGGA GCTGGAGAAC 
TACACGGACT GCCCGGAGCT GGTTGGCAGG TGCTTTCTGG AGCGGATGGA GGAGTTCCAG 
ATCTACGAGA AGTACTGTCA GAACAAGCCG CGCTCCGAGA GCCTGTGGAG ACAGTGCTCC 
GACTGCCCCT TCTTCCAGGA GTGCCAGAAG AAGCTGGACC ACAAGCTGAG CCTGGACTCC 
TACCTGCTGA AGCCAGTCCA GAGGATCACC AAGTACCAGC TGCTGCTGAA GGAGATGCTT 
AAATACAGCA AGAGTTGCGA GGGGGCGGAG GACCTGCAGG AGGCCCTGAG CTCCATCCTG 
GGCATCCTCA AGGCCGTGAA CGACTCCATG CACCTGATCG CCATCACCGG CTACGAGGGG 
AACCTGAGTG ACCTGGGGAG GCTGCTCATG CAGGGCTCCT TCAGTGTGTG GACCGACCAC 
AAGAGGGGCC ACGCCAAGGT GAAGGACCTG GCCCGCTTCA AGCCCATGCA GCGCCACCTG 
TTCCTGCACG AGAAGGCCGT GCTCTTCTGT AAGCGGCGGG AGGAGAACGG CGAGGGCTAC 
GAGAAGGCGC CCTCCTACAG CTACAAGCAG TCCCTGAACA TGACGGCCGT CGGCATCACG 
GAGAACGTGA AAGGAGACGC CAAGAAGTTT GAGATCTGGT ACAACGCCCG GGAGGAGGTG 
TACATCATCC AGGCACCCAC TCCTGAAATC AAGGCCGCCT GGGTGAGCGA GATCCGGAAG 
GTGCTGACCA GCCAGCTGCA GGCATGCAGA GAGGCCAGCC AGCATCGCGC CCTCGAGCAG 
TCCCAGAGCC TGCCCCTGCC CACACCAGCT GGCACCAGTC CCTCGAAAGC GAGCACAAGA 
AACATAAAAA AGTTAGAAGA AAGAAAAACG GACCCGCTGA GCCTGGAGGG ATACGGGGGG 
CCGACACCAC AGCAGAGGGC CCCCGAGAAG GGCAAAGGCT GGAGCAAAAC GTCCCACCCA 
TCAGAGACGC CTGAGGACAA CGATGGCTGG TCCAGCACCG AGGAGCCCGT CAACTCCTCG 
GACGCGGAGG AGGAGGGCAG AGCGGGCCCC GGGAAGCTGG TCCCCGGGAG GTACACCGTC 
ACCAGGCTGG ACGAGAAGGG GGTCTCCGAC GTGCTGGCCC TGAGAAGCGG GGACGAGGTG 
GAGCTCGTGC AGGAGGGAGA TGAGGGCCTC TGGTGCGTGC GGAACCTGAG CTCCGGCGTC 
GAGGGCTGGG TGCCCGCCCG CAGCCTGTCC ACTCTCCTCT GCCAGGGTGG CCCCGTGGGG 
TGCCTGAGCA GCCCAGAGTC CAGTGCGGGT TCCGCGGTGC TGAGCCCCTC GTCCAGCTGC 
AGTGAGAGCT GCACGGCCGC CCTCGCTGAC CTGCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_010810996.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010809298.1 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X19, mRNA.

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CloneID	OBa46308
Clone ID Related Accession (Same CDS sequence)	XM_010810996.2 , XM_010822296.2
Accession Version	XM_010810996.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810996.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT GGCTGAGACA TGAAGAGATG GCCCTGGAGG AAATGGTGCA GAGATTGAAT 
GCGGTTTCCA AGCGCACTGA TGAGATCATG CACCACGACA TCAGCCCGCT CTGTGCCGCC 
GACATCCGGG ACCAGCTGCA GAAGCGCTTC GCTTACCTGT CAGGCGGGCG GGGCCAGGAC 
GGCAGCCCGG TCATCACCTT TCCCGACTAC CCGGCCTTCG GAGACGTCCC AGACCAGGAC 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGG 
TTCATCCTGG TCATAGACCG CAGGCAGGAC AGATGGACCT CGGTCAAGGC GTCCATCCTG 
AGAATAGCGG CATCCTTCCC CGCAAACCTG CAGCTCGTCC TCGTCCTGCG GCCAACCGGG 
TTCTTCCAGA GGACACTCTC CGACCTCGCC TTCAGGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCAGTC CCAGAACTAC ACGGCTACAT CGACAAGTCC 
CAGCTGACGG AGGACCTGGG TGGGACGCTG GCCTACTGCC ACTCCAGGTG GCTCCGCCAT 
CGCACAGCCA TCGAGAGCTT CGCCCTCCTG GTCAAGCAGA CGGCGCAGAT GCTGCAGACC 
TTTGGGACCG AGCTGGCCGA GACGGAGCTG CCCAATGACG TGCAGTCCAC GAGCTCCGTG 
CTCCTCGCCC ACACGGAGAA GAAGGACAGA GCCAAGGAGG ATATGAGGCT GGCGCTTGTC 
GAGGGGCGGC GCGTCCTGGA GAGCATCCAC GAGCCACTGG CCCGGAGCCC AGATCAGAGC 
CCCAACCAGG ACCAACTGGA CAGTCAGAGC ACCGTGCACA GGCTCCTGGC CCAGCTGAAC 
GAGACCGAGG CCGCCTTTGA CGAGTTCTGG GCAAAACATC AGCAAAAACT GGAGCAGTGC 
CTGCAGCTCC GGCATTTCGA GCAGGACTTC CGAGAGGTCA AATCGGCCCT GGACGTGCTG 
GCCCAGAAGG TCACGACTTT CACCGACGTG GGCAACAGCC TGGCACACGT GCAGCACCTC 
CTGAAGGACC TGACCAGCTT CGAGGAGAAG TCCAGTGCCG CCGTGCAGCG GGCCCGCACG 
CTGTCCCAGG AGGGTGAGCG GCTCATCGAG CACAAGCACT ATGCCGTGGA CTCCATCCGC 
CCCAAGTGTC ACGAGCTCCG CCACCTGTGC GACCAGTTCG CGGCCGACGT GGAGCGGAGG 
AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG CACGGCCTCC TGGAGGCGTC CATGAAGTGG 
TGTGACGAGG GCATCTACCT GCTGGCATCG CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT 
GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG AGGTTTCTGG AGACGGGGGC AGAAAATAAG 
ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC TACGAGCCCA TCCTCACCAA AGACCTGCTG 
GAGCATGTGC AGAGGGTCTT CCAGAAGCAG GAGAGCGTGG AGGAGATGTT CCACCGCAGG 
CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG CAGACTCGGC CCGTGCAGCC CGTGGCCCCC 
CGGCCGGAAG CACTCACCAA GTCGCCCTGC CCCTCCCCAG GCATCCGGAG GGGTTCTGAG 
AACCACAGTT CCGAGGGCAG TGCACTCCGG AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG 
GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC TCCACGGGGG ACGAGGAGAG CCTGGCCGTC 
CTGCGGAGGC ATGTGATGAA GGAGCTTCTG GACACAGAGC GGGTCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGACAACC CGTTAATGAC TCATCTCATC 
TCCACTGGCC TTCAGAACAA GAAGGACATC CTGTTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GGATATTCCT GAGGGAGCTG GAGAACTACA CGGACTGCCC GGAGCTGGTT 
GGCAGGTGCT TTCTGGAGCG GATGGAGGAG TTCCAGATCT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGCT CCGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCCTTCTT CCAGGAGTGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTGAAGGAG ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG 
GCGGAGGACC TGCAGGAGGC CCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TGATCGCCAT CACCGGCTAC GAGGGGAACC TGAGTGACCT GGGGAGGCTG 
CTCATGCAGG GCTCCTTCAG TGTGTGGACC GACCACAAGA GGGGCCACGC CAAGGTGAAG 
GACCTGGCCC GCTTCAAGCC CATGCAGCGC CACCTGTTCC TGCACGAGAA GGCCGTGCTC 
TTCTGTAAGC GGCGGGAGGA GAACGGCGAG GGCTACGAGA AGGCGCCCTC CTACAGCTAC 
AAGCAGTCCC TGAACATGAC GGCCGTCGGC ATCACGGAGA ACGTGAAAGG AGACGCCAAG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTGTACA TCATCCAGGC ACCCACTCCT 
GAAATCAAGG CCGCCTGGGT GAGCGAGATC CGGAAGGTGC TGACCAGCCA GCTGCAGGCA 
TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC GAGCAGTCCC AGAGCCTGCC CCTGCCCACA 
CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC ACAAGAAACA TAAAAAAGTT AGAAGAAAGA 
AAAACGGACC CGCTGAGCCT GGAGGGATAC GGGGGGCCGA CACCACAGCA GAGGGCCCCC 
GAGAAGGGCA AAGATGACGC GGTCACTAGC TCTACCTCAG AAAGCTCTGC CCTTTCTAAA 
AAGCGCTTCA CCCTGCAGAG CTTTGCTGCC CTCAAAGCCC AGAAAGCTTC TCCCACCAGT 
CCTGACAAAA AAGCTAAGCG ACACCAAGTA AAGAGCGACC CCACGCCTTT TGGGTTACGA 
GGCTGGAGCA AAACGTCCCA CCCATCAGAG ACGCCTGAGG ACAACGATGG CTGGTCCAGC 
ACCGAGGAGC CCGTCAACTC CTCGGACGCG GAGGAGGAGG GCAGAGCGGG CCCCGGGAAG 
CTGGTCCCCG GGAGGTACAC CGTCACCAGG CTGGACGAGA AGGGGGTCTC CGACGTGCTG 
GCCCTGAGAA GCGGGGACGA GGTGGAGCTC GTGCAGGAGG GAGATGAGGG CCTCTGGTGC 
GTGCGGAACC TGAGCTCCGG CGTCGAGGGC TGGGTGCCCG CCCGCAGCCT GTCCACTCTC 
CTCTGCCAGG GTGGCCCCGT GGGGTGCCTG AGCAGCCCAG AGTCCAGTGC GGGTTCCGCG 
GTGCTGAGCC CCTCGTCCAG CTGCAGTGAG AGCTGCACGG CCGCCCTCGC TGACCTGCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010809298.1
CDS	91..3636
Translation	MRFWLRHEEMALEEMVQRLNAVSKRTDEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKDDAVTSSTSESSALSKKRFTLQSFAALKAQKASPTSPDKKAKRHQVKSDPTPFGLRGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPARSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_010810996.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810996.2

ORF Insert Sequence:

ATGAGGTTCT GGCTGAGACA TGAAGAGATG GCCCTGGAGG AAATGGTGCA GAGATTGAAT 
GCGGTTTCCA AGCGCACTGA TGAGATCATG CACCACGACA TCAGCCCGCT CTGTGCCGCC 
GACATCCGGG ACCAGCTGCA GAAGCGCTTC GCTTACCTGT CAGGCGGGCG GGGCCAGGAC 
GGCAGCCCGG TCATCACCTT TCCCGACTAC CCGGCCTTCG GAGACGTCCC AGACCAGGAC 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGG 
TTCATCCTGG TCATAGACCG CAGGCAGGAC AGATGGACCT CGGTCAAGGC GTCCATCCTG 
AGAATAGCGG CATCCTTCCC CGCAAACCTG CAGCTCGTCC TCGTCCTGCG GCCAACCGGG 
TTCTTCCAGA GGACACTCTC CGACCTCGCC TTCAGGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCAGTC CCAGAACTAC ACGGCTACAT CGACAAGTCC 
CAGCTGACGG AGGACCTGGG TGGGACGCTG GCCTACTGCC ACTCCAGGTG GCTCCGCCAT 
CGCACAGCCA TCGAGAGCTT CGCCCTCCTG GTCAAGCAGA CGGCGCAGAT GCTGCAGACC 
TTTGGGACCG AGCTGGCCGA GACGGAGCTG CCCAATGACG TGCAGTCCAC GAGCTCCGTG 
CTCCTCGCCC ACACGGAGAA GAAGGACAGA GCCAAGGAGG ATATGAGGCT GGCGCTTGTC 
GAGGGGCGGC GCGTCCTGGA GAGCATCCAC GAGCCACTGG CCCGGAGCCC AGATCAGAGC 
CCCAACCAGG ACCAACTGGA CAGTCAGAGC ACCGTGCACA GGCTCCTGGC CCAGCTGAAC 
GAGACCGAGG CCGCCTTTGA CGAGTTCTGG GCAAAACATC AGCAAAAACT GGAGCAGTGC 
CTGCAGCTCC GGCATTTCGA GCAGGACTTC CGAGAGGTCA AATCGGCCCT GGACGTGCTG 
GCCCAGAAGG TCACGACTTT CACCGACGTG GGCAACAGCC TGGCACACGT GCAGCACCTC 
CTGAAGGACC TGACCAGCTT CGAGGAGAAG TCCAGTGCCG CCGTGCAGCG GGCCCGCACG 
CTGTCCCAGG AGGGTGAGCG GCTCATCGAG CACAAGCACT ATGCCGTGGA CTCCATCCGC 
CCCAAGTGTC ACGAGCTCCG CCACCTGTGC GACCAGTTCG CGGCCGACGT GGAGCGGAGG 
AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG CACGGCCTCC TGGAGGCGTC CATGAAGTGG 
TGTGACGAGG GCATCTACCT GCTGGCATCG CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT 
GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG AGGTTTCTGG AGACGGGGGC AGAAAATAAG 
ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC TACGAGCCCA TCCTCACCAA AGACCTGCTG 
GAGCATGTGC AGAGGGTCTT CCAGAAGCAG GAGAGCGTGG AGGAGATGTT CCACCGCAGG 
CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG CAGACTCGGC CCGTGCAGCC CGTGGCCCCC 
CGGCCGGAAG CACTCACCAA GTCGCCCTGC CCCTCCCCAG GCATCCGGAG GGGTTCTGAG 
AACCACAGTT CCGAGGGCAG TGCACTCCGG AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG 
GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC TCCACGGGGG ACGAGGAGAG CCTGGCCGTC 
CTGCGGAGGC ATGTGATGAA GGAGCTTCTG GACACAGAGC GGGTCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGACAACC CGTTAATGAC TCATCTCATC 
TCCACTGGCC TTCAGAACAA GAAGGACATC CTGTTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GGATATTCCT GAGGGAGCTG GAGAACTACA CGGACTGCCC GGAGCTGGTT 
GGCAGGTGCT TTCTGGAGCG GATGGAGGAG TTCCAGATCT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGCT CCGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCCTTCTT CCAGGAGTGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTGAAGGAG ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG 
GCGGAGGACC TGCAGGAGGC CCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TGATCGCCAT CACCGGCTAC GAGGGGAACC TGAGTGACCT GGGGAGGCTG 
CTCATGCAGG GCTCCTTCAG TGTGTGGACC GACCACAAGA GGGGCCACGC CAAGGTGAAG 
GACCTGGCCC GCTTCAAGCC CATGCAGCGC CACCTGTTCC TGCACGAGAA GGCCGTGCTC 
TTCTGTAAGC GGCGGGAGGA GAACGGCGAG GGCTACGAGA AGGCGCCCTC CTACAGCTAC 
AAGCAGTCCC TGAACATGAC GGCCGTCGGC ATCACGGAGA ACGTGAAAGG AGACGCCAAG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTGTACA TCATCCAGGC ACCCACTCCT 
GAAATCAAGG CCGCCTGGGT GAGCGAGATC CGGAAGGTGC TGACCAGCCA GCTGCAGGCA 
TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC GAGCAGTCCC AGAGCCTGCC CCTGCCCACA 
CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC ACAAGAAACA TAAAAAAGTT AGAAGAAAGA 
AAAACGGACC CGCTGAGCCT GGAGGGATAC GGGGGGCCGA CACCACAGCA GAGGGCCCCC 
GAGAAGGGCA AAGATGACGC GGTCACTAGC TCTACCTCAG AAAGCTCTGC CCTTTCTAAA 
AAGCGCTTCA CCCTGCAGAG CTTTGCTGCC CTCAAAGCCC AGAAAGCTTC TCCCACCAGT 
CCTGACAAAA AAGCTAAGCG ACACCAAGTA AAGAGCGACC CCACGCCTTT TGGGTTACGA 
GGCTGGAGCA AAACGTCCCA CCCATCAGAG ACGCCTGAGG ACAACGATGG CTGGTCCAGC 
ACCGAGGAGC CCGTCAACTC CTCGGACGCG GAGGAGGAGG GCAGAGCGGG CCCCGGGAAG 
CTGGTCCCCG GGAGGTACAC CGTCACCAGG CTGGACGAGA AGGGGGTCTC CGACGTGCTG 
GCCCTGAGAA GCGGGGACGA GGTGGAGCTC GTGCAGGAGG GAGATGAGGG CCTCTGGTGC 
GTGCGGAACC TGAGCTCCGG CGTCGAGGGC TGGGTGCCCG CCCGCAGCCT GTCCACTCTC 
CTCTGCCAGG GTGGCCCCGT GGGGTGCCTG AGCAGCCCAG AGTCCAGTGC GGGTTCCGCG 
GTGCTGAGCC CCTCGTCCAG CTGCAGTGAG AGCTGCACGG CCGCCCTCGC TGACCTGCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_010822296.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820598.1 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X19, mRNA.

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CloneID	OBa46308
Clone ID Related Accession (Same CDS sequence)	XM_010810996.2 , XM_010822296.2
Accession Version	XM_010822296.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646112.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822296.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT GGCTGAGACA TGAAGAGATG GCCCTGGAGG AAATGGTGCA GAGATTGAAT 
GCGGTTTCCA AGCGCACTGA TGAGATCATG CACCACGACA TCAGCCCGCT CTGTGCCGCC 
GACATCCGGG ACCAGCTGCA GAAGCGCTTC GCTTACCTGT CAGGCGGGCG GGGCCAGGAC 
GGCAGCCCGG TCATCACCTT TCCCGACTAC CCGGCCTTCG GAGACGTCCC AGACCAGGAC 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGG 
TTCATCCTGG TCATAGACCG CAGGCAGGAC AGATGGACCT CGGTCAAGGC GTCCATCCTG 
AGAATAGCGG CATCCTTCCC CGCAAACCTG CAGCTCGTCC TCGTCCTGCG GCCAACCGGG 
TTCTTCCAGA GGACACTCTC CGACCTCGCC TTCAGGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCAGTC CCAGAACTAC ACGGCTACAT CGACAAGTCC 
CAGCTGACGG AGGACCTGGG TGGGACGCTG GCCTACTGCC ACTCCAGGTG GCTCCGCCAT 
CGCACAGCCA TCGAGAGCTT CGCCCTCCTG GTCAAGCAGA CGGCGCAGAT GCTGCAGACC 
TTTGGGACCG AGCTGGCCGA GACGGAGCTG CCCAATGACG TGCAGTCCAC GAGCTCCGTG 
CTCCTCGCCC ACACGGAGAA GAAGGACAGA GCCAAGGAGG ATATGAGGCT GGCGCTTGTC 
GAGGGGCGGC GCGTCCTGGA GAGCATCCAC GAGCCACTGG CCCGGAGCCC AGATCAGAGC 
CCCAACCAGG ACCAACTGGA CAGTCAGAGC ACCGTGCACA GGCTCCTGGC CCAGCTGAAC 
GAGACCGAGG CCGCCTTTGA CGAGTTCTGG GCAAAACATC AGCAAAAACT GGAGCAGTGC 
CTGCAGCTCC GGCATTTCGA GCAGGACTTC CGAGAGGTCA AATCGGCCCT GGACGTGCTG 
GCCCAGAAGG TCACGACTTT CACCGACGTG GGCAACAGCC TGGCACACGT GCAGCACCTC 
CTGAAGGACC TGACCAGCTT CGAGGAGAAG TCCAGTGCCG CCGTGCAGCG GGCCCGCACG 
CTGTCCCAGG AGGGTGAGCG GCTCATCGAG CACAAGCACT ATGCCGTGGA CTCCATCCGC 
CCCAAGTGTC ACGAGCTCCG CCACCTGTGC GACCAGTTCG CGGCCGACGT GGAGCGGAGG 
AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG CACGGCCTCC TGGAGGCGTC CATGAAGTGG 
TGTGACGAGG GCATCTACCT GCTGGCATCG CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT 
GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG AGGTTTCTGG AGACGGGGGC AGAAAATAAG 
ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC TACGAGCCCA TCCTCACCAA AGACCTGCTG 
GAGCATGTGC AGAGGGTCTT CCAGAAGCAG GAGAGCGTGG AGGAGATGTT CCACCGCAGG 
CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG CAGACTCGGC CCGTGCAGCC CGTGGCCCCC 
CGGCCGGAAG CACTCACCAA GTCGCCCTGC CCCTCCCCAG GCATCCGGAG GGGTTCTGAG 
AACCACAGTT CCGAGGGCAG TGCACTCCGG AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG 
GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC TCCACGGGGG ACGAGGAGAG CCTGGCCGTC 
CTGCGGAGGC ATGTGATGAA GGAGCTTCTG GACACAGAGC GGGTCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGACAACC CGTTAATGAC TCATCTCATC 
TCCACTGGCC TTCAGAACAA GAAGGACATC CTGTTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GGATATTCCT GAGGGAGCTG GAGAACTACA CGGACTGCCC GGAGCTGGTT 
GGCAGGTGCT TTCTGGAGCG GATGGAGGAG TTCCAGATCT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGCT CCGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCCTTCTT CCAGGAGTGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTGAAGGAG ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG 
GCGGAGGACC TGCAGGAGGC CCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TGATCGCCAT CACCGGCTAC GAGGGGAACC TGAGTGACCT GGGGAGGCTG 
CTCATGCAGG GCTCCTTCAG TGTGTGGACC GACCACAAGA GGGGCCACGC CAAGGTGAAG 
GACCTGGCCC GCTTCAAGCC CATGCAGCGC CACCTGTTCC TGCACGAGAA GGCCGTGCTC 
TTCTGTAAGC GGCGGGAGGA GAACGGCGAG GGCTACGAGA AGGCGCCCTC CTACAGCTAC 
AAGCAGTCCC TGAACATGAC GGCCGTCGGC ATCACGGAGA ACGTGAAAGG AGACGCCAAG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTGTACA TCATCCAGGC ACCCACTCCT 
GAAATCAAGG CCGCCTGGGT GAGCGAGATC CGGAAGGTGC TGACCAGCCA GCTGCAGGCA 
TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC GAGCAGTCCC AGAGCCTGCC CCTGCCCACA 
CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC ACAAGAAACA TAAAAAAGTT AGAAGAAAGA 
AAAACGGACC CGCTGAGCCT GGAGGGATAC GGGGGGCCGA CACCACAGCA GAGGGCCCCC 
GAGAAGGGCA AAGATGACGC GGTCACTAGC TCTACCTCAG AAAGCTCTGC CCTTTCTAAA 
AAGCGCTTCA CCCTGCAGAG CTTTGCTGCC CTCAAAGCCC AGAAAGCTTC TCCCACCAGT 
CCTGACAAAA AAGCTAAGCG ACACCAAGTA AAGAGCGACC CCACGCCTTT TGGGTTACGA 
GGCTGGAGCA AAACGTCCCA CCCATCAGAG ACGCCTGAGG ACAACGATGG CTGGTCCAGC 
ACCGAGGAGC CCGTCAACTC CTCGGACGCG GAGGAGGAGG GCAGAGCGGG CCCCGGGAAG 
CTGGTCCCCG GGAGGTACAC CGTCACCAGG CTGGACGAGA AGGGGGTCTC CGACGTGCTG 
GCCCTGAGAA GCGGGGACGA GGTGGAGCTC GTGCAGGAGG GAGATGAGGG CCTCTGGTGC 
GTGCGGAACC TGAGCTCCGG CGTCGAGGGC TGGGTGCCCG CCCGCAGCCT GTCCACTCTC 
CTCTGCCAGG GTGGCCCCGT GGGGTGCCTG AGCAGCCCAG AGTCCAGTGC GGGTTCCGCG 
GTGCTGAGCC CCTCGTCCAG CTGCAGTGAG AGCTGCACGG CCGCCCTCGC TGACCTGCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820598.1
CDS	91..3636
Translation	MRFWLRHEEMALEEMVQRLNAVSKRTDEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKDDAVTSSTSESSALSKKRFTLQSFAALKAQKASPTSPDKKAKRHQVKSDPTPFGLRGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPARSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_010822296.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822296.2

ORF Insert Sequence:

ATGAGGTTCT GGCTGAGACA TGAAGAGATG GCCCTGGAGG AAATGGTGCA GAGATTGAAT 
GCGGTTTCCA AGCGCACTGA TGAGATCATG CACCACGACA TCAGCCCGCT CTGTGCCGCC 
GACATCCGGG ACCAGCTGCA GAAGCGCTTC GCTTACCTGT CAGGCGGGCG GGGCCAGGAC 
GGCAGCCCGG TCATCACCTT TCCCGACTAC CCGGCCTTCG GAGACGTCCC AGACCAGGAC 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGG 
TTCATCCTGG TCATAGACCG CAGGCAGGAC AGATGGACCT CGGTCAAGGC GTCCATCCTG 
AGAATAGCGG CATCCTTCCC CGCAAACCTG CAGCTCGTCC TCGTCCTGCG GCCAACCGGG 
TTCTTCCAGA GGACACTCTC CGACCTCGCC TTCAGGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCAGTC CCAGAACTAC ACGGCTACAT CGACAAGTCC 
CAGCTGACGG AGGACCTGGG TGGGACGCTG GCCTACTGCC ACTCCAGGTG GCTCCGCCAT 
CGCACAGCCA TCGAGAGCTT CGCCCTCCTG GTCAAGCAGA CGGCGCAGAT GCTGCAGACC 
TTTGGGACCG AGCTGGCCGA GACGGAGCTG CCCAATGACG TGCAGTCCAC GAGCTCCGTG 
CTCCTCGCCC ACACGGAGAA GAAGGACAGA GCCAAGGAGG ATATGAGGCT GGCGCTTGTC 
GAGGGGCGGC GCGTCCTGGA GAGCATCCAC GAGCCACTGG CCCGGAGCCC AGATCAGAGC 
CCCAACCAGG ACCAACTGGA CAGTCAGAGC ACCGTGCACA GGCTCCTGGC CCAGCTGAAC 
GAGACCGAGG CCGCCTTTGA CGAGTTCTGG GCAAAACATC AGCAAAAACT GGAGCAGTGC 
CTGCAGCTCC GGCATTTCGA GCAGGACTTC CGAGAGGTCA AATCGGCCCT GGACGTGCTG 
GCCCAGAAGG TCACGACTTT CACCGACGTG GGCAACAGCC TGGCACACGT GCAGCACCTC 
CTGAAGGACC TGACCAGCTT CGAGGAGAAG TCCAGTGCCG CCGTGCAGCG GGCCCGCACG 
CTGTCCCAGG AGGGTGAGCG GCTCATCGAG CACAAGCACT ATGCCGTGGA CTCCATCCGC 
CCCAAGTGTC ACGAGCTCCG CCACCTGTGC GACCAGTTCG CGGCCGACGT GGAGCGGAGG 
AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG CACGGCCTCC TGGAGGCGTC CATGAAGTGG 
TGTGACGAGG GCATCTACCT GCTGGCATCG CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT 
GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG AGGTTTCTGG AGACGGGGGC AGAAAATAAG 
ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC TACGAGCCCA TCCTCACCAA AGACCTGCTG 
GAGCATGTGC AGAGGGTCTT CCAGAAGCAG GAGAGCGTGG AGGAGATGTT CCACCGCAGG 
CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG CAGACTCGGC CCGTGCAGCC CGTGGCCCCC 
CGGCCGGAAG CACTCACCAA GTCGCCCTGC CCCTCCCCAG GCATCCGGAG GGGTTCTGAG 
AACCACAGTT CCGAGGGCAG TGCACTCCGG AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG 
GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC TCCACGGGGG ACGAGGAGAG CCTGGCCGTC 
CTGCGGAGGC ATGTGATGAA GGAGCTTCTG GACACAGAGC GGGTCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGACAACC CGTTAATGAC TCATCTCATC 
TCCACTGGCC TTCAGAACAA GAAGGACATC CTGTTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GGATATTCCT GAGGGAGCTG GAGAACTACA CGGACTGCCC GGAGCTGGTT 
GGCAGGTGCT TTCTGGAGCG GATGGAGGAG TTCCAGATCT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGCT CCGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCCTTCTT CCAGGAGTGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTGAAGGAG ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG 
GCGGAGGACC TGCAGGAGGC CCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TGATCGCCAT CACCGGCTAC GAGGGGAACC TGAGTGACCT GGGGAGGCTG 
CTCATGCAGG GCTCCTTCAG TGTGTGGACC GACCACAAGA GGGGCCACGC CAAGGTGAAG 
GACCTGGCCC GCTTCAAGCC CATGCAGCGC CACCTGTTCC TGCACGAGAA GGCCGTGCTC 
TTCTGTAAGC GGCGGGAGGA GAACGGCGAG GGCTACGAGA AGGCGCCCTC CTACAGCTAC 
AAGCAGTCCC TGAACATGAC GGCCGTCGGC ATCACGGAGA ACGTGAAAGG AGACGCCAAG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTGTACA TCATCCAGGC ACCCACTCCT 
GAAATCAAGG CCGCCTGGGT GAGCGAGATC CGGAAGGTGC TGACCAGCCA GCTGCAGGCA 
TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC GAGCAGTCCC AGAGCCTGCC CCTGCCCACA 
CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC ACAAGAAACA TAAAAAAGTT AGAAGAAAGA 
AAAACGGACC CGCTGAGCCT GGAGGGATAC GGGGGGCCGA CACCACAGCA GAGGGCCCCC 
GAGAAGGGCA AAGATGACGC GGTCACTAGC TCTACCTCAG AAAGCTCTGC CCTTTCTAAA 
AAGCGCTTCA CCCTGCAGAG CTTTGCTGCC CTCAAAGCCC AGAAAGCTTC TCCCACCAGT 
CCTGACAAAA AAGCTAAGCG ACACCAAGTA AAGAGCGACC CCACGCCTTT TGGGTTACGA 
GGCTGGAGCA AAACGTCCCA CCCATCAGAG ACGCCTGAGG ACAACGATGG CTGGTCCAGC 
ACCGAGGAGC CCGTCAACTC CTCGGACGCG GAGGAGGAGG GCAGAGCGGG CCCCGGGAAG 
CTGGTCCCCG GGAGGTACAC CGTCACCAGG CTGGACGAGA AGGGGGTCTC CGACGTGCTG 
GCCCTGAGAA GCGGGGACGA GGTGGAGCTC GTGCAGGAGG GAGATGAGGG CCTCTGGTGC 
GTGCGGAACC TGAGCTCCGG CGTCGAGGGC TGGGTGCCCG CCCGCAGCCT GTCCACTCTC 
CTCTGCCAGG GTGGCCCCGT GGGGTGCCTG AGCAGCCCAG AGTCCAGTGC GGGTTCCGCG 
GTGCTGAGCC CCTCGTCCAG CTGCAGTGAG AGCTGCACGG CCGCCCTCGC TGACCTGCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_010822297.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820599.1 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OBa46309
Clone ID Related Accession (Same CDS sequence)	XM_010822297.2 , XM_010810997.2
Accession Version	XM_010822297.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646112.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822297.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT GGCTGAGACA TGAAGAGATG GCCCTGGAGG AAATGGTGCA GAGATTGAAT 
GCGGTTTCCA AGCGCACTGA TGAGATCATG CACCACGACA TCAGCCCGCT CTGTGCCGCC 
GACATCCGGG ACCAGCTGCA GAAGCGCTTC GCTTACCTGT CAGGCGGGCG GGGCCAGGAC 
GGCAGCCCGG TCATCACCTT TCCCGACTAC CCGGCCTTCG GAGACGTCCC AGACCAGGAC 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGG 
TTCATCCTGG TCATAGACCG CAGGCAGGAC AGATGGACCT CGGTCAAGGC GTCCATCCTG 
AGAATAGCGG CATCCTTCCC CGCAAACCTG CAGCTCGTCC TCGTCCTGCG GCCAACCGGG 
TTCTTCCAGA GGACACTCTC CGACCTCGCC TTCAGGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCAGTC CCAGAACTAC ACGGCTACAT CGACAAGTCC 
CAGCTGACGG AGGACCTGGG TGGGACGCTG GCCTACTGCC ACTCCAGGTG GCTCCGCCAT 
CGCACAGCCA TCGAGAGCTT CGCCCTCCTG GTCAAGCAGA CGGCGCAGAT GCTGCAGACC 
TTTGGGACCG AGCTGGCCGA GACGGAGCTG CCCAATGACG TGCAGTCCAC GAGCTCCGTG 
CTCCTCGCCC ACACGGAGAA GAAGGACAGA GCCAAGGAGG ATATGAGGCT GGCGCTTGTC 
GAGGGGCGGC GCGTCCTGGA GAGCATCCAC GAGCCACTGG CCCGGAGCCC AGATCAGAGC 
CCCAACCAGG ACCAACTGGA CAGTCAGAGC ACCGTGCACA GGCTCCTGGC CCAGCTGAAC 
GAGACCGAGG CCGCCTTTGA CGAGTTCTGG GCAAAACATC AGCAAAAACT GGAGCAGTGC 
CTGCAGCTCC GGCATTTCGA GCAGGACTTC CGAGAGGTCA AATCGGCCCT GGACGTGCTG 
GCCCAGAAGG TCACGACTTT CACCGACGTG GGCAACAGCC TGGCACACGT GCAGCACCTC 
CTGAAGGACC TGACCAGCTT CGAGGAGAAG TCCAGTGCCG CCGTGCAGCG GGCCCGCACG 
CTGTCCCAGG AGGGTGAGCG GCTCATCGAG CACAAGCACT ATGCCGTGGA CTCCATCCGC 
CCCAAGTGTC ACGAGCTCCG CCACCTGTGC GACCAGTTCG CGGCCGACGT GGAGCGGAGG 
AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG CACGGCCTCC TGGAGGCGTC CATGAAGTGG 
TGTGACGAGG GCATCTACCT GCTGGCATCG CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT 
GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG AGGTTTCTGG AGACGGGGGC AGAAAATAAG 
ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC TACGAGCCCA TCCTCACCAA AGACCTGCTG 
GAGCATGTGC AGAGGGTCTT CCAGAAGCAG GAGAGCGTGG AGGAGATGTT CCACCGCAGG 
CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG CAGACTCGGC CCGTGCAGCC CGTGGCCCCC 
CGGCCGGAAG CACTCACCAA GTCGCCCTGC CCCTCCCCAG GCATCCGGAG GGGTTCTGAG 
AACCACAGTT CCGAGGGCAG TGCACTCCGG AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG 
GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC TCCACGGGGG ACGAGGAGAG CCTGGCCGTC 
CTGCGGAGGC ATGTGATGAA GGAGCTTCTG GACACAGAGC GGGTCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGACAACC CGTTAATGAC TCATCTCATC 
TCCACTGGCC TTCAGAACAA GAAGGACATC CTGTTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GGATATTCCT GAGGGAGCTG GAGAACTACA CGGACTGCCC GGAGCTGGTT 
GGCAGGTGCT TTCTGGAGCG GATGGAGGAG TTCCAGATCT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGCT CCGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCCTTCTT CCAGGAGTGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTGAAGGAG ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG 
GCGGAGGACC TGCAGGAGGC CCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TGATCGCCAT CACCGGCTAC GAGGGGAACC TGAGTGACCT GGGGAGGCTG 
CTCATGCAGG GCTCCTTCAG TGTGTGGACC GACCACAAGA GGGGCCACGC CAAGGTGAAG 
GACCTGGCCC GCTTCAAGCC CATGCAGCGC CACCTGTTCC TGCACGAGAA GGCCGTGCTC 
TTCTGTAAGC GGCGGGAGGA GAACGGCGAG GGCTACGAGA AGGCGCCCTC CTACAGCTAC 
AAGCAGTCCC TGAACATGAC GGCCGTCGGC ATCACGGAGA ACGTGAAAGG AGACGCCAAG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTGTACA TCATCCAGGC ACCCACTCCT 
GAAATCAAGG CCGCCTGGGT GAGCGAGATC CGGAAGGTGC TGACCAGCCA GCTGCAGGCA 
TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC GAGCAGTCCC AGAGCCTGCC CCTGCCCACA 
CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC ACAAGAAACA TAAAAAAGTT AGAAGAAAGA 
AAAACGGACC CGCTGAGCCT GGAGGGATAC GGGGGGCCGA CACCACAGCA GAGGGCCCCC 
GAGAAGGGCA AAGGCTGGAG CAAAACGTCC CACCCATCAG AGACGCCTGA GGACAACGAT 
GGCTGGTCCA GCACCGAGGA GCCCGTCAAC TCCTCGGACG CGGAGGAGGA GGGCAGAGCG 
GGCCCCGGGA AGCTGGTCCC CGGGAGGTAC ACCGTCACCA GGCTGGACGA GAAGGGGGTC 
TCCGACGTGC TGGCCCTGAG AAGCGGGGAC GAGGTGGAGC TCGTGCAGGA GGGAGATGAG 
GGCCTCTGGT GCGTGCGGAA CCTGAGCTCC GGCGTCGAGG GCTGGGTGCC CGCCCGCAGC 
CTGTCCACTC TCCTCTGCCA GGGTGGCCCC GTGGGGTGCC TGAGCAGCCC AGAGTCCAGT 
GCGGGTTCCG CGGTGCTGAG CCCCTCGTCC AGCTGCAGTG AGAGCTGCAC GGCCGCCCTC 
GCTGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820599.1
CDS	91..3468
Translation	MRFWLRHEEMALEEMVQRLNAVSKRTDEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPARSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_010822297.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822297.2

ORF Insert Sequence:

ATGAGGTTCT GGCTGAGACA TGAAGAGATG GCCCTGGAGG AAATGGTGCA GAGATTGAAT 
GCGGTTTCCA AGCGCACTGA TGAGATCATG CACCACGACA TCAGCCCGCT CTGTGCCGCC 
GACATCCGGG ACCAGCTGCA GAAGCGCTTC GCTTACCTGT CAGGCGGGCG GGGCCAGGAC 
GGCAGCCCGG TCATCACCTT TCCCGACTAC CCGGCCTTCG GAGACGTCCC AGACCAGGAC 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGG 
TTCATCCTGG TCATAGACCG CAGGCAGGAC AGATGGACCT CGGTCAAGGC GTCCATCCTG 
AGAATAGCGG CATCCTTCCC CGCAAACCTG CAGCTCGTCC TCGTCCTGCG GCCAACCGGG 
TTCTTCCAGA GGACACTCTC CGACCTCGCC TTCAGGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCAGTC CCAGAACTAC ACGGCTACAT CGACAAGTCC 
CAGCTGACGG AGGACCTGGG TGGGACGCTG GCCTACTGCC ACTCCAGGTG GCTCCGCCAT 
CGCACAGCCA TCGAGAGCTT CGCCCTCCTG GTCAAGCAGA CGGCGCAGAT GCTGCAGACC 
TTTGGGACCG AGCTGGCCGA GACGGAGCTG CCCAATGACG TGCAGTCCAC GAGCTCCGTG 
CTCCTCGCCC ACACGGAGAA GAAGGACAGA GCCAAGGAGG ATATGAGGCT GGCGCTTGTC 
GAGGGGCGGC GCGTCCTGGA GAGCATCCAC GAGCCACTGG CCCGGAGCCC AGATCAGAGC 
CCCAACCAGG ACCAACTGGA CAGTCAGAGC ACCGTGCACA GGCTCCTGGC CCAGCTGAAC 
GAGACCGAGG CCGCCTTTGA CGAGTTCTGG GCAAAACATC AGCAAAAACT GGAGCAGTGC 
CTGCAGCTCC GGCATTTCGA GCAGGACTTC CGAGAGGTCA AATCGGCCCT GGACGTGCTG 
GCCCAGAAGG TCACGACTTT CACCGACGTG GGCAACAGCC TGGCACACGT GCAGCACCTC 
CTGAAGGACC TGACCAGCTT CGAGGAGAAG TCCAGTGCCG CCGTGCAGCG GGCCCGCACG 
CTGTCCCAGG AGGGTGAGCG GCTCATCGAG CACAAGCACT ATGCCGTGGA CTCCATCCGC 
CCCAAGTGTC ACGAGCTCCG CCACCTGTGC GACCAGTTCG CGGCCGACGT GGAGCGGAGG 
AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG CACGGCCTCC TGGAGGCGTC CATGAAGTGG 
TGTGACGAGG GCATCTACCT GCTGGCATCG CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT 
GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG AGGTTTCTGG AGACGGGGGC AGAAAATAAG 
ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC TACGAGCCCA TCCTCACCAA AGACCTGCTG 
GAGCATGTGC AGAGGGTCTT CCAGAAGCAG GAGAGCGTGG AGGAGATGTT CCACCGCAGG 
CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG CAGACTCGGC CCGTGCAGCC CGTGGCCCCC 
CGGCCGGAAG CACTCACCAA GTCGCCCTGC CCCTCCCCAG GCATCCGGAG GGGTTCTGAG 
AACCACAGTT CCGAGGGCAG TGCACTCCGG AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG 
GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC TCCACGGGGG ACGAGGAGAG CCTGGCCGTC 
CTGCGGAGGC ATGTGATGAA GGAGCTTCTG GACACAGAGC GGGTCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGACAACC CGTTAATGAC TCATCTCATC 
TCCACTGGCC TTCAGAACAA GAAGGACATC CTGTTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GGATATTCCT GAGGGAGCTG GAGAACTACA CGGACTGCCC GGAGCTGGTT 
GGCAGGTGCT TTCTGGAGCG GATGGAGGAG TTCCAGATCT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGCT CCGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCCTTCTT CCAGGAGTGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTGAAGGAG ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG 
GCGGAGGACC TGCAGGAGGC CCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TGATCGCCAT CACCGGCTAC GAGGGGAACC TGAGTGACCT GGGGAGGCTG 
CTCATGCAGG GCTCCTTCAG TGTGTGGACC GACCACAAGA GGGGCCACGC CAAGGTGAAG 
GACCTGGCCC GCTTCAAGCC CATGCAGCGC CACCTGTTCC TGCACGAGAA GGCCGTGCTC 
TTCTGTAAGC GGCGGGAGGA GAACGGCGAG GGCTACGAGA AGGCGCCCTC CTACAGCTAC 
AAGCAGTCCC TGAACATGAC GGCCGTCGGC ATCACGGAGA ACGTGAAAGG AGACGCCAAG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTGTACA TCATCCAGGC ACCCACTCCT 
GAAATCAAGG CCGCCTGGGT GAGCGAGATC CGGAAGGTGC TGACCAGCCA GCTGCAGGCA 
TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC GAGCAGTCCC AGAGCCTGCC CCTGCCCACA 
CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC ACAAGAAACA TAAAAAAGTT AGAAGAAAGA 
AAAACGGACC CGCTGAGCCT GGAGGGATAC GGGGGGCCGA CACCACAGCA GAGGGCCCCC 
GAGAAGGGCA AAGGCTGGAG CAAAACGTCC CACCCATCAG AGACGCCTGA GGACAACGAT 
GGCTGGTCCA GCACCGAGGA GCCCGTCAAC TCCTCGGACG CGGAGGAGGA GGGCAGAGCG 
GGCCCCGGGA AGCTGGTCCC CGGGAGGTAC ACCGTCACCA GGCTGGACGA GAAGGGGGTC 
TCCGACGTGC TGGCCCTGAG AAGCGGGGAC GAGGTGGAGC TCGTGCAGGA GGGAGATGAG 
GGCCTCTGGT GCGTGCGGAA CCTGAGCTCC GGCGTCGAGG GCTGGGTGCC CGCCCGCAGC 
CTGTCCACTC TCCTCTGCCA GGGTGGCCCC GTGGGGTGCC TGAGCAGCCC AGAGTCCAGT 
GCGGGTTCCG CGGTGCTGAG CCCCTCGTCC AGCTGCAGTG AGAGCTGCAC GGCCGCCCTC 
GCTGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_010810997.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010809299.1 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OBa46309
Clone ID Related Accession (Same CDS sequence)	XM_010822297.2 , XM_010810997.2
Accession Version	XM_010810997.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810997.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT GGCTGAGACA TGAAGAGATG GCCCTGGAGG AAATGGTGCA GAGATTGAAT 
GCGGTTTCCA AGCGCACTGA TGAGATCATG CACCACGACA TCAGCCCGCT CTGTGCCGCC 
GACATCCGGG ACCAGCTGCA GAAGCGCTTC GCTTACCTGT CAGGCGGGCG GGGCCAGGAC 
GGCAGCCCGG TCATCACCTT TCCCGACTAC CCGGCCTTCG GAGACGTCCC AGACCAGGAC 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGG 
TTCATCCTGG TCATAGACCG CAGGCAGGAC AGATGGACCT CGGTCAAGGC GTCCATCCTG 
AGAATAGCGG CATCCTTCCC CGCAAACCTG CAGCTCGTCC TCGTCCTGCG GCCAACCGGG 
TTCTTCCAGA GGACACTCTC CGACCTCGCC TTCAGGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCAGTC CCAGAACTAC ACGGCTACAT CGACAAGTCC 
CAGCTGACGG AGGACCTGGG TGGGACGCTG GCCTACTGCC ACTCCAGGTG GCTCCGCCAT 
CGCACAGCCA TCGAGAGCTT CGCCCTCCTG GTCAAGCAGA CGGCGCAGAT GCTGCAGACC 
TTTGGGACCG AGCTGGCCGA GACGGAGCTG CCCAATGACG TGCAGTCCAC GAGCTCCGTG 
CTCCTCGCCC ACACGGAGAA GAAGGACAGA GCCAAGGAGG ATATGAGGCT GGCGCTTGTC 
GAGGGGCGGC GCGTCCTGGA GAGCATCCAC GAGCCACTGG CCCGGAGCCC AGATCAGAGC 
CCCAACCAGG ACCAACTGGA CAGTCAGAGC ACCGTGCACA GGCTCCTGGC CCAGCTGAAC 
GAGACCGAGG CCGCCTTTGA CGAGTTCTGG GCAAAACATC AGCAAAAACT GGAGCAGTGC 
CTGCAGCTCC GGCATTTCGA GCAGGACTTC CGAGAGGTCA AATCGGCCCT GGACGTGCTG 
GCCCAGAAGG TCACGACTTT CACCGACGTG GGCAACAGCC TGGCACACGT GCAGCACCTC 
CTGAAGGACC TGACCAGCTT CGAGGAGAAG TCCAGTGCCG CCGTGCAGCG GGCCCGCACG 
CTGTCCCAGG AGGGTGAGCG GCTCATCGAG CACAAGCACT ATGCCGTGGA CTCCATCCGC 
CCCAAGTGTC ACGAGCTCCG CCACCTGTGC GACCAGTTCG CGGCCGACGT GGAGCGGAGG 
AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG CACGGCCTCC TGGAGGCGTC CATGAAGTGG 
TGTGACGAGG GCATCTACCT GCTGGCATCG CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT 
GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG AGGTTTCTGG AGACGGGGGC AGAAAATAAG 
ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC TACGAGCCCA TCCTCACCAA AGACCTGCTG 
GAGCATGTGC AGAGGGTCTT CCAGAAGCAG GAGAGCGTGG AGGAGATGTT CCACCGCAGG 
CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG CAGACTCGGC CCGTGCAGCC CGTGGCCCCC 
CGGCCGGAAG CACTCACCAA GTCGCCCTGC CCCTCCCCAG GCATCCGGAG GGGTTCTGAG 
AACCACAGTT CCGAGGGCAG TGCACTCCGG AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG 
GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC TCCACGGGGG ACGAGGAGAG CCTGGCCGTC 
CTGCGGAGGC ATGTGATGAA GGAGCTTCTG GACACAGAGC GGGTCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGACAACC CGTTAATGAC TCATCTCATC 
TCCACTGGCC TTCAGAACAA GAAGGACATC CTGTTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GGATATTCCT GAGGGAGCTG GAGAACTACA CGGACTGCCC GGAGCTGGTT 
GGCAGGTGCT TTCTGGAGCG GATGGAGGAG TTCCAGATCT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGCT CCGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCCTTCTT CCAGGAGTGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTGAAGGAG ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG 
GCGGAGGACC TGCAGGAGGC CCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TGATCGCCAT CACCGGCTAC GAGGGGAACC TGAGTGACCT GGGGAGGCTG 
CTCATGCAGG GCTCCTTCAG TGTGTGGACC GACCACAAGA GGGGCCACGC CAAGGTGAAG 
GACCTGGCCC GCTTCAAGCC CATGCAGCGC CACCTGTTCC TGCACGAGAA GGCCGTGCTC 
TTCTGTAAGC GGCGGGAGGA GAACGGCGAG GGCTACGAGA AGGCGCCCTC CTACAGCTAC 
AAGCAGTCCC TGAACATGAC GGCCGTCGGC ATCACGGAGA ACGTGAAAGG AGACGCCAAG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTGTACA TCATCCAGGC ACCCACTCCT 
GAAATCAAGG CCGCCTGGGT GAGCGAGATC CGGAAGGTGC TGACCAGCCA GCTGCAGGCA 
TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC GAGCAGTCCC AGAGCCTGCC CCTGCCCACA 
CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC ACAAGAAACA TAAAAAAGTT AGAAGAAAGA 
AAAACGGACC CGCTGAGCCT GGAGGGATAC GGGGGGCCGA CACCACAGCA GAGGGCCCCC 
GAGAAGGGCA AAGGCTGGAG CAAAACGTCC CACCCATCAG AGACGCCTGA GGACAACGAT 
GGCTGGTCCA GCACCGAGGA GCCCGTCAAC TCCTCGGACG CGGAGGAGGA GGGCAGAGCG 
GGCCCCGGGA AGCTGGTCCC CGGGAGGTAC ACCGTCACCA GGCTGGACGA GAAGGGGGTC 
TCCGACGTGC TGGCCCTGAG AAGCGGGGAC GAGGTGGAGC TCGTGCAGGA GGGAGATGAG 
GGCCTCTGGT GCGTGCGGAA CCTGAGCTCC GGCGTCGAGG GCTGGGTGCC CGCCCGCAGC 
CTGTCCACTC TCCTCTGCCA GGGTGGCCCC GTGGGGTGCC TGAGCAGCCC AGAGTCCAGT 
GCGGGTTCCG CGGTGCTGAG CCCCTCGTCC AGCTGCAGTG AGAGCTGCAC GGCCGCCCTC 
GCTGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010809299.1
CDS	91..3468
Translation	MRFWLRHEEMALEEMVQRLNAVSKRTDEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPARSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_010810997.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810997.2

ORF Insert Sequence:

ATGAGGTTCT GGCTGAGACA TGAAGAGATG GCCCTGGAGG AAATGGTGCA GAGATTGAAT 
GCGGTTTCCA AGCGCACTGA TGAGATCATG CACCACGACA TCAGCCCGCT CTGTGCCGCC 
GACATCCGGG ACCAGCTGCA GAAGCGCTTC GCTTACCTGT CAGGCGGGCG GGGCCAGGAC 
GGCAGCCCGG TCATCACCTT TCCCGACTAC CCGGCCTTCG GAGACGTCCC AGACCAGGAC 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGG 
TTCATCCTGG TCATAGACCG CAGGCAGGAC AGATGGACCT CGGTCAAGGC GTCCATCCTG 
AGAATAGCGG CATCCTTCCC CGCAAACCTG CAGCTCGTCC TCGTCCTGCG GCCAACCGGG 
TTCTTCCAGA GGACACTCTC CGACCTCGCC TTCAGGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCAGTC CCAGAACTAC ACGGCTACAT CGACAAGTCC 
CAGCTGACGG AGGACCTGGG TGGGACGCTG GCCTACTGCC ACTCCAGGTG GCTCCGCCAT 
CGCACAGCCA TCGAGAGCTT CGCCCTCCTG GTCAAGCAGA CGGCGCAGAT GCTGCAGACC 
TTTGGGACCG AGCTGGCCGA GACGGAGCTG CCCAATGACG TGCAGTCCAC GAGCTCCGTG 
CTCCTCGCCC ACACGGAGAA GAAGGACAGA GCCAAGGAGG ATATGAGGCT GGCGCTTGTC 
GAGGGGCGGC GCGTCCTGGA GAGCATCCAC GAGCCACTGG CCCGGAGCCC AGATCAGAGC 
CCCAACCAGG ACCAACTGGA CAGTCAGAGC ACCGTGCACA GGCTCCTGGC CCAGCTGAAC 
GAGACCGAGG CCGCCTTTGA CGAGTTCTGG GCAAAACATC AGCAAAAACT GGAGCAGTGC 
CTGCAGCTCC GGCATTTCGA GCAGGACTTC CGAGAGGTCA AATCGGCCCT GGACGTGCTG 
GCCCAGAAGG TCACGACTTT CACCGACGTG GGCAACAGCC TGGCACACGT GCAGCACCTC 
CTGAAGGACC TGACCAGCTT CGAGGAGAAG TCCAGTGCCG CCGTGCAGCG GGCCCGCACG 
CTGTCCCAGG AGGGTGAGCG GCTCATCGAG CACAAGCACT ATGCCGTGGA CTCCATCCGC 
CCCAAGTGTC ACGAGCTCCG CCACCTGTGC GACCAGTTCG CGGCCGACGT GGAGCGGAGG 
AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG CACGGCCTCC TGGAGGCGTC CATGAAGTGG 
TGTGACGAGG GCATCTACCT GCTGGCATCG CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT 
GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG AGGTTTCTGG AGACGGGGGC AGAAAATAAG 
ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC TACGAGCCCA TCCTCACCAA AGACCTGCTG 
GAGCATGTGC AGAGGGTCTT CCAGAAGCAG GAGAGCGTGG AGGAGATGTT CCACCGCAGG 
CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG CAGACTCGGC CCGTGCAGCC CGTGGCCCCC 
CGGCCGGAAG CACTCACCAA GTCGCCCTGC CCCTCCCCAG GCATCCGGAG GGGTTCTGAG 
AACCACAGTT CCGAGGGCAG TGCACTCCGG AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG 
GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC TCCACGGGGG ACGAGGAGAG CCTGGCCGTC 
CTGCGGAGGC ATGTGATGAA GGAGCTTCTG GACACAGAGC GGGTCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGACAACC CGTTAATGAC TCATCTCATC 
TCCACTGGCC TTCAGAACAA GAAGGACATC CTGTTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GGATATTCCT GAGGGAGCTG GAGAACTACA CGGACTGCCC GGAGCTGGTT 
GGCAGGTGCT TTCTGGAGCG GATGGAGGAG TTCCAGATCT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGCT CCGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCCTTCTT CCAGGAGTGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTGAAGGAG ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG 
GCGGAGGACC TGCAGGAGGC CCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TGATCGCCAT CACCGGCTAC GAGGGGAACC TGAGTGACCT GGGGAGGCTG 
CTCATGCAGG GCTCCTTCAG TGTGTGGACC GACCACAAGA GGGGCCACGC CAAGGTGAAG 
GACCTGGCCC GCTTCAAGCC CATGCAGCGC CACCTGTTCC TGCACGAGAA GGCCGTGCTC 
TTCTGTAAGC GGCGGGAGGA GAACGGCGAG GGCTACGAGA AGGCGCCCTC CTACAGCTAC 
AAGCAGTCCC TGAACATGAC GGCCGTCGGC ATCACGGAGA ACGTGAAAGG AGACGCCAAG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTGTACA TCATCCAGGC ACCCACTCCT 
GAAATCAAGG CCGCCTGGGT GAGCGAGATC CGGAAGGTGC TGACCAGCCA GCTGCAGGCA 
TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC GAGCAGTCCC AGAGCCTGCC CCTGCCCACA 
CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC ACAAGAAACA TAAAAAAGTT AGAAGAAAGA 
AAAACGGACC CGCTGAGCCT GGAGGGATAC GGGGGGCCGA CACCACAGCA GAGGGCCCCC 
GAGAAGGGCA AAGGCTGGAG CAAAACGTCC CACCCATCAG AGACGCCTGA GGACAACGAT 
GGCTGGTCCA GCACCGAGGA GCCCGTCAAC TCCTCGGACG CGGAGGAGGA GGGCAGAGCG 
GGCCCCGGGA AGCTGGTCCC CGGGAGGTAC ACCGTCACCA GGCTGGACGA GAAGGGGGTC 
TCCGACGTGC TGGCCCTGAG AAGCGGGGAC GAGGTGGAGC TCGTGCAGGA GGGAGATGAG 
GGCCTCTGGT GCGTGCGGAA CCTGAGCTCC GGCGTCGAGG GCTGGGTGCC CGCCCGCAGC 
CTGTCCACTC TCCTCTGCCA GGGTGGCCCC GTGGGGTGCC TGAGCAGCCC AGAGTCCAGT 
GCGGGTTCCG CGGTGCTGAG CCCCTCGTCC AGCTGCAGTG AGAGCTGCAC GGCCGCCCTC 
GCTGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_010810998.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010809300.1 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OBa46310
Clone ID Related Accession (Same CDS sequence)	XM_010810998.2 , XM_010822298.2
Accession Version	XM_010810998.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGACT GTTGGAGGCT CATCCTTTGC CAGAAAACCG GAAGCGACGA TGCTTCTTCC 
TCGCGAGGGT CCCACGAGGC CGAAGAGGAG GACAGCGACC CTCGGGTGGA CGTCCCTGAC 
GTCATCAGGC TTGTTCCAGA CCCGCCGGAG GGGCCACCCC TGCCTGCAGA TGAGATCATG 
CACCACGACA TCAGCCCGCT CTGTGCCGCC GACATCCGGG ACCAGCTGCA GAAGCGCTTC 
GCTTACCTGT CAGGCGGGCG GGGCCAGGAC GGCAGCCCGG TCATCACCTT TCCCGACTAC 
CCGGCCTTCG GAGACGTCCC AGACCAGGAC TTCCAGAATG TCATGACCTA CCTCACCAGC 
ATCCCCAGCC TGCAGGACGC TGGCATCGGG TTCATCCTGG TCATAGACCG CAGGCAGGAC 
AGATGGACCT CGGTCAAGGC GTCCATCCTG AGAATAGCGG CATCCTTCCC CGCAAACCTG 
CAGCTCGTCC TCGTCCTGCG GCCAACCGGG TTCTTCCAGA GGACACTCTC CGACCTCGCC 
TTCAGGTTCA ACAGAGATGA CTTTAAGATG AAGGTGCCGG TCATAATGCT GAGCTCAGTC 
CCAGAACTAC ACGGCTACAT CGACAAGTCC CAGCTGACGG AGGACCTGGG TGGGACGCTG 
GCCTACTGCC ACTCCAGGTG GCTCCGCCAT CGCACAGCCA TCGAGAGCTT CGCCCTCCTG 
GTCAAGCAGA CGGCGCAGAT GCTGCAGACC TTTGGGACCG AGCTGGCCGA GACGGAGCTG 
CCCAATGACG TGCAGTCCAC GAGCTCCGTG CTCCTCGCCC ACACGGAGAA GAAGGACAGA 
GCCAAGGAGG ATATGAGGCT GGCGCTTGTC GAGGGGCGGC GCGTCCTGGA GAGCATCCAC 
GAGCCACTGG CCCGGAGCCC AGATCAGAGC CCCAACCAGG ACCAACTGGA CAGTCAGAGC 
ACCGTGCACA GGCTCCTGGC CCAGCTGAAC GAGACCGAGG CCGCCTTTGA CGAGTTCTGG 
GCAAAACATC AGCAAAAACT GGAGCAGTGC CTGCAGCTCC GGCATTTCGA GCAGGACTTC 
CGAGAGGTCA AATCGGCCCT GGACGTGCTG GCCCAGAAGG TCACGACTTT CACCGACGTG 
GGCAACAGCC TGGCACACGT GCAGCACCTC CTGAAGGACC TGACCAGCTT CGAGGAGAAG 
TCCAGTGCCG CCGTGCAGCG GGCCCGCACG CTGTCCCAGG AGGGTGAGCG GCTCATCGAG 
CACAAGCACT ATGCCGTGGA CTCCATCCGC CCCAAGTGTC ACGAGCTCCG CCACCTGTGC 
GACCAGTTCG CGGCCGACGT GGAGCGGAGG AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG 
CACGGCCTCC TGGAGGCGTC CATGAAGTGG TGTGACGAGG GCATCTACCT GCTGGCATCG 
CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG 
AGGTTTCTGG AGACGGGGGC AGAAAATAAG ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC 
TACGAGCCCA TCCTCACCAA AGACCTGCTG GAGCATGTGC AGAGGGTCTT CCAGAAGCAG 
GAGAGCGTGG AGGAGATGTT CCACCGCAGG CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG 
CAGACTCGGC CCGTGCAGCC CGTGGCCCCC CGGCCGGAAG CACTCACCAA GTCGCCCTGC 
CCCTCCCCAG GCATCCGGAG GGGTTCTGAG AACCACAGTT CCGAGGGCAG TGCACTCCGG 
AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC 
TCCACGGGGG ACGAGGAGAG CCTGGCCGTC CTGCGGAGGC ATGTGATGAA GGAGCTTCTG 
GACACAGAGC GGGTCTACGT GGAGGAGCTG CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG 
ATGGACAACC CGTTAATGAC TCATCTCATC TCCACTGGCC TTCAGAACAA GAAGGACATC 
CTGTTTGGAA ACATGGAAGA AATTTACCAC TTTCATAACA GGATATTCCT GAGGGAGCTG 
GAGAACTACA CGGACTGCCC GGAGCTGGTT GGCAGGTGCT TTCTGGAGCG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCGCGCT CCGAGAGCCT GTGGAGACAG 
TGCTCCGACT GCCCCTTCTT CCAGGAGTGC CAGAAGAAGC TGGACCACAA GCTGAGCCTG 
GACTCCTACC TGCTGAAGCC AGTCCAGAGG ATCACCAAGT ACCAGCTGCT GCTGAAGGAG 
ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG GCGGAGGACC TGCAGGAGGC CCTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC CGTGAACGAC TCCATGCACC TGATCGCCAT CACCGGCTAC 
GAGGGGAACC TGAGTGACCT GGGGAGGCTG CTCATGCAGG GCTCCTTCAG TGTGTGGACC 
GACCACAAGA GGGGCCACGC CAAGGTGAAG GACCTGGCCC GCTTCAAGCC CATGCAGCGC 
CACCTGTTCC TGCACGAGAA GGCCGTGCTC TTCTGTAAGC GGCGGGAGGA GAACGGCGAG 
GGCTACGAGA AGGCGCCCTC CTACAGCTAC AAGCAGTCCC TGAACATGAC GGCCGTCGGC 
ATCACGGAGA ACGTGAAAGG AGACGCCAAG AAGTTTGAGA TCTGGTACAA CGCCCGGGAG 
GAGGTGTACA TCATCCAGGC ACCCACTCCT GAAATCAAGG CCGCCTGGGT GAGCGAGATC 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCA TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC 
GAGCAGTCCC AGAGCCTGCC CCTGCCCACA CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC 
ACAAGAAACA TAAAAAAGTT AGAAGAAAGA AAAACGGACC CGCTGAGCCT GGAGGGATAC 
GGGGGGCCGA CACCACAGCA GAGGGCCCCC GAGAAGGGCA AAGGCTGGAG CAAAACGTCC 
CACCCATCAG AGACGCCTGA GGACAACGAT GGCTGGTCCA GCACCGAGGA GCCCGTCAAC 
TCCTCGGACG CGGAGGAGGA GGGCAGAGCG GGCCCCGGGA AGCTGGTCCC CGGGAGGTAC 
ACCGTCACCA GGCTGGACGA GAAGGGGGTC TCCGACGTGC TGGCCCTGAG AAGCGGGGAC 
GAGGTGGAGC TCGTGCAGGA GGGAGATGAG GGCCTCTGGT GCGTGCGGAA CCTGAGCTCC 
GGCGTCGAGG GCTGGGTGCC CGCCCGCAGC CTGTCCACTC TCCTCTGCCA GGGTGGCCCC 
GTGGGGTGCC TGAGCAGCCC AGAGTCCAGT GCGGGTTCCG CGGTGCTGAG CCCCTCGTCC 
AGCTGCAGTG AGAGCTGCAC GGCCGCCCTC GCTGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010809300.1
CDS	84..3551
Translation	MFDCWRLILCQKTGSDDASSSRGSHEAEEEDSDPRVDVPDVIRLVPDPPEGPPLPADEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPARSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_010810998.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810998.2

ORF Insert Sequence:

ATGTTCGACT GTTGGAGGCT CATCCTTTGC CAGAAAACCG GAAGCGACGA TGCTTCTTCC 
TCGCGAGGGT CCCACGAGGC CGAAGAGGAG GACAGCGACC CTCGGGTGGA CGTCCCTGAC 
GTCATCAGGC TTGTTCCAGA CCCGCCGGAG GGGCCACCCC TGCCTGCAGA TGAGATCATG 
CACCACGACA TCAGCCCGCT CTGTGCCGCC GACATCCGGG ACCAGCTGCA GAAGCGCTTC 
GCTTACCTGT CAGGCGGGCG GGGCCAGGAC GGCAGCCCGG TCATCACCTT TCCCGACTAC 
CCGGCCTTCG GAGACGTCCC AGACCAGGAC TTCCAGAATG TCATGACCTA CCTCACCAGC 
ATCCCCAGCC TGCAGGACGC TGGCATCGGG TTCATCCTGG TCATAGACCG CAGGCAGGAC 
AGATGGACCT CGGTCAAGGC GTCCATCCTG AGAATAGCGG CATCCTTCCC CGCAAACCTG 
CAGCTCGTCC TCGTCCTGCG GCCAACCGGG TTCTTCCAGA GGACACTCTC CGACCTCGCC 
TTCAGGTTCA ACAGAGATGA CTTTAAGATG AAGGTGCCGG TCATAATGCT GAGCTCAGTC 
CCAGAACTAC ACGGCTACAT CGACAAGTCC CAGCTGACGG AGGACCTGGG TGGGACGCTG 
GCCTACTGCC ACTCCAGGTG GCTCCGCCAT CGCACAGCCA TCGAGAGCTT CGCCCTCCTG 
GTCAAGCAGA CGGCGCAGAT GCTGCAGACC TTTGGGACCG AGCTGGCCGA GACGGAGCTG 
CCCAATGACG TGCAGTCCAC GAGCTCCGTG CTCCTCGCCC ACACGGAGAA GAAGGACAGA 
GCCAAGGAGG ATATGAGGCT GGCGCTTGTC GAGGGGCGGC GCGTCCTGGA GAGCATCCAC 
GAGCCACTGG CCCGGAGCCC AGATCAGAGC CCCAACCAGG ACCAACTGGA CAGTCAGAGC 
ACCGTGCACA GGCTCCTGGC CCAGCTGAAC GAGACCGAGG CCGCCTTTGA CGAGTTCTGG 
GCAAAACATC AGCAAAAACT GGAGCAGTGC CTGCAGCTCC GGCATTTCGA GCAGGACTTC 
CGAGAGGTCA AATCGGCCCT GGACGTGCTG GCCCAGAAGG TCACGACTTT CACCGACGTG 
GGCAACAGCC TGGCACACGT GCAGCACCTC CTGAAGGACC TGACCAGCTT CGAGGAGAAG 
TCCAGTGCCG CCGTGCAGCG GGCCCGCACG CTGTCCCAGG AGGGTGAGCG GCTCATCGAG 
CACAAGCACT ATGCCGTGGA CTCCATCCGC CCCAAGTGTC ACGAGCTCCG CCACCTGTGC 
GACCAGTTCG CGGCCGACGT GGAGCGGAGG AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG 
CACGGCCTCC TGGAGGCGTC CATGAAGTGG TGTGACGAGG GCATCTACCT GCTGGCATCG 
CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG 
AGGTTTCTGG AGACGGGGGC AGAAAATAAG ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC 
TACGAGCCCA TCCTCACCAA AGACCTGCTG GAGCATGTGC AGAGGGTCTT CCAGAAGCAG 
GAGAGCGTGG AGGAGATGTT CCACCGCAGG CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG 
CAGACTCGGC CCGTGCAGCC CGTGGCCCCC CGGCCGGAAG CACTCACCAA GTCGCCCTGC 
CCCTCCCCAG GCATCCGGAG GGGTTCTGAG AACCACAGTT CCGAGGGCAG TGCACTCCGG 
AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC 
TCCACGGGGG ACGAGGAGAG CCTGGCCGTC CTGCGGAGGC ATGTGATGAA GGAGCTTCTG 
GACACAGAGC GGGTCTACGT GGAGGAGCTG CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG 
ATGGACAACC CGTTAATGAC TCATCTCATC TCCACTGGCC TTCAGAACAA GAAGGACATC 
CTGTTTGGAA ACATGGAAGA AATTTACCAC TTTCATAACA GGATATTCCT GAGGGAGCTG 
GAGAACTACA CGGACTGCCC GGAGCTGGTT GGCAGGTGCT TTCTGGAGCG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCGCGCT CCGAGAGCCT GTGGAGACAG 
TGCTCCGACT GCCCCTTCTT CCAGGAGTGC CAGAAGAAGC TGGACCACAA GCTGAGCCTG 
GACTCCTACC TGCTGAAGCC AGTCCAGAGG ATCACCAAGT ACCAGCTGCT GCTGAAGGAG 
ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG GCGGAGGACC TGCAGGAGGC CCTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC CGTGAACGAC TCCATGCACC TGATCGCCAT CACCGGCTAC 
GAGGGGAACC TGAGTGACCT GGGGAGGCTG CTCATGCAGG GCTCCTTCAG TGTGTGGACC 
GACCACAAGA GGGGCCACGC CAAGGTGAAG GACCTGGCCC GCTTCAAGCC CATGCAGCGC 
CACCTGTTCC TGCACGAGAA GGCCGTGCTC TTCTGTAAGC GGCGGGAGGA GAACGGCGAG 
GGCTACGAGA AGGCGCCCTC CTACAGCTAC AAGCAGTCCC TGAACATGAC GGCCGTCGGC 
ATCACGGAGA ACGTGAAAGG AGACGCCAAG AAGTTTGAGA TCTGGTACAA CGCCCGGGAG 
GAGGTGTACA TCATCCAGGC ACCCACTCCT GAAATCAAGG CCGCCTGGGT GAGCGAGATC 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCA TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC 
GAGCAGTCCC AGAGCCTGCC CCTGCCCACA CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC 
ACAAGAAACA TAAAAAAGTT AGAAGAAAGA AAAACGGACC CGCTGAGCCT GGAGGGATAC 
GGGGGGCCGA CACCACAGCA GAGGGCCCCC GAGAAGGGCA AAGGCTGGAG CAAAACGTCC 
CACCCATCAG AGACGCCTGA GGACAACGAT GGCTGGTCCA GCACCGAGGA GCCCGTCAAC 
TCCTCGGACG CGGAGGAGGA GGGCAGAGCG GGCCCCGGGA AGCTGGTCCC CGGGAGGTAC 
ACCGTCACCA GGCTGGACGA GAAGGGGGTC TCCGACGTGC TGGCCCTGAG AAGCGGGGAC 
GAGGTGGAGC TCGTGCAGGA GGGAGATGAG GGCCTCTGGT GCGTGCGGAA CCTGAGCTCC 
GGCGTCGAGG GCTGGGTGCC CGCCCGCAGC CTGTCCACTC TCCTCTGCCA GGGTGGCCCC 
GTGGGGTGCC TGAGCAGCCC AGAGTCCAGT GCGGGTTCCG CGGTGCTGAG CCCCTCGTCC 
AGCTGCAGTG AGAGCTGCAC GGCCGCCCTC GCTGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_010822298.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820600.1 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X21, mRNA.

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CloneID	OBa46310
Clone ID Related Accession (Same CDS sequence)	XM_010810998.2 , XM_010822298.2
Accession Version	XM_010822298.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646112.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822298.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGACT GTTGGAGGCT CATCCTTTGC CAGAAAACCG GAAGCGACGA TGCTTCTTCC 
TCGCGAGGGT CCCACGAGGC CGAAGAGGAG GACAGCGACC CTCGGGTGGA CGTCCCTGAC 
GTCATCAGGC TTGTTCCAGA CCCGCCGGAG GGGCCACCCC TGCCTGCAGA TGAGATCATG 
CACCACGACA TCAGCCCGCT CTGTGCCGCC GACATCCGGG ACCAGCTGCA GAAGCGCTTC 
GCTTACCTGT CAGGCGGGCG GGGCCAGGAC GGCAGCCCGG TCATCACCTT TCCCGACTAC 
CCGGCCTTCG GAGACGTCCC AGACCAGGAC TTCCAGAATG TCATGACCTA CCTCACCAGC 
ATCCCCAGCC TGCAGGACGC TGGCATCGGG TTCATCCTGG TCATAGACCG CAGGCAGGAC 
AGATGGACCT CGGTCAAGGC GTCCATCCTG AGAATAGCGG CATCCTTCCC CGCAAACCTG 
CAGCTCGTCC TCGTCCTGCG GCCAACCGGG TTCTTCCAGA GGACACTCTC CGACCTCGCC 
TTCAGGTTCA ACAGAGATGA CTTTAAGATG AAGGTGCCGG TCATAATGCT GAGCTCAGTC 
CCAGAACTAC ACGGCTACAT CGACAAGTCC CAGCTGACGG AGGACCTGGG TGGGACGCTG 
GCCTACTGCC ACTCCAGGTG GCTCCGCCAT CGCACAGCCA TCGAGAGCTT CGCCCTCCTG 
GTCAAGCAGA CGGCGCAGAT GCTGCAGACC TTTGGGACCG AGCTGGCCGA GACGGAGCTG 
CCCAATGACG TGCAGTCCAC GAGCTCCGTG CTCCTCGCCC ACACGGAGAA GAAGGACAGA 
GCCAAGGAGG ATATGAGGCT GGCGCTTGTC GAGGGGCGGC GCGTCCTGGA GAGCATCCAC 
GAGCCACTGG CCCGGAGCCC AGATCAGAGC CCCAACCAGG ACCAACTGGA CAGTCAGAGC 
ACCGTGCACA GGCTCCTGGC CCAGCTGAAC GAGACCGAGG CCGCCTTTGA CGAGTTCTGG 
GCAAAACATC AGCAAAAACT GGAGCAGTGC CTGCAGCTCC GGCATTTCGA GCAGGACTTC 
CGAGAGGTCA AATCGGCCCT GGACGTGCTG GCCCAGAAGG TCACGACTTT CACCGACGTG 
GGCAACAGCC TGGCACACGT GCAGCACCTC CTGAAGGACC TGACCAGCTT CGAGGAGAAG 
TCCAGTGCCG CCGTGCAGCG GGCCCGCACG CTGTCCCAGG AGGGTGAGCG GCTCATCGAG 
CACAAGCACT ATGCCGTGGA CTCCATCCGC CCCAAGTGTC ACGAGCTCCG CCACCTGTGC 
GACCAGTTCG CGGCCGACGT GGAGCGGAGG AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG 
CACGGCCTCC TGGAGGCGTC CATGAAGTGG TGTGACGAGG GCATCTACCT GCTGGCATCG 
CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG 
AGGTTTCTGG AGACGGGGGC AGAAAATAAG ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC 
TACGAGCCCA TCCTCACCAA AGACCTGCTG GAGCATGTGC AGAGGGTCTT CCAGAAGCAG 
GAGAGCGTGG AGGAGATGTT CCACCGCAGG CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG 
CAGACTCGGC CCGTGCAGCC CGTGGCCCCC CGGCCGGAAG CACTCACCAA GTCGCCCTGC 
CCCTCCCCAG GCATCCGGAG GGGTTCTGAG AACCACAGTT CCGAGGGCAG TGCACTCCGG 
AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC 
TCCACGGGGG ACGAGGAGAG CCTGGCCGTC CTGCGGAGGC ATGTGATGAA GGAGCTTCTG 
GACACAGAGC GGGTCTACGT GGAGGAGCTG CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG 
ATGGACAACC CGTTAATGAC TCATCTCATC TCCACTGGCC TTCAGAACAA GAAGGACATC 
CTGTTTGGAA ACATGGAAGA AATTTACCAC TTTCATAACA GGATATTCCT GAGGGAGCTG 
GAGAACTACA CGGACTGCCC GGAGCTGGTT GGCAGGTGCT TTCTGGAGCG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCGCGCT CCGAGAGCCT GTGGAGACAG 
TGCTCCGACT GCCCCTTCTT CCAGGAGTGC CAGAAGAAGC TGGACCACAA GCTGAGCCTG 
GACTCCTACC TGCTGAAGCC AGTCCAGAGG ATCACCAAGT ACCAGCTGCT GCTGAAGGAG 
ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG GCGGAGGACC TGCAGGAGGC CCTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC CGTGAACGAC TCCATGCACC TGATCGCCAT CACCGGCTAC 
GAGGGGAACC TGAGTGACCT GGGGAGGCTG CTCATGCAGG GCTCCTTCAG TGTGTGGACC 
GACCACAAGA GGGGCCACGC CAAGGTGAAG GACCTGGCCC GCTTCAAGCC CATGCAGCGC 
CACCTGTTCC TGCACGAGAA GGCCGTGCTC TTCTGTAAGC GGCGGGAGGA GAACGGCGAG 
GGCTACGAGA AGGCGCCCTC CTACAGCTAC AAGCAGTCCC TGAACATGAC GGCCGTCGGC 
ATCACGGAGA ACGTGAAAGG AGACGCCAAG AAGTTTGAGA TCTGGTACAA CGCCCGGGAG 
GAGGTGTACA TCATCCAGGC ACCCACTCCT GAAATCAAGG CCGCCTGGGT GAGCGAGATC 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCA TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC 
GAGCAGTCCC AGAGCCTGCC CCTGCCCACA CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC 
ACAAGAAACA TAAAAAAGTT AGAAGAAAGA AAAACGGACC CGCTGAGCCT GGAGGGATAC 
GGGGGGCCGA CACCACAGCA GAGGGCCCCC GAGAAGGGCA AAGGCTGGAG CAAAACGTCC 
CACCCATCAG AGACGCCTGA GGACAACGAT GGCTGGTCCA GCACCGAGGA GCCCGTCAAC 
TCCTCGGACG CGGAGGAGGA GGGCAGAGCG GGCCCCGGGA AGCTGGTCCC CGGGAGGTAC 
ACCGTCACCA GGCTGGACGA GAAGGGGGTC TCCGACGTGC TGGCCCTGAG AAGCGGGGAC 
GAGGTGGAGC TCGTGCAGGA GGGAGATGAG GGCCTCTGGT GCGTGCGGAA CCTGAGCTCC 
GGCGTCGAGG GCTGGGTGCC CGCCCGCAGC CTGTCCACTC TCCTCTGCCA GGGTGGCCCC 
GTGGGGTGCC TGAGCAGCCC AGAGTCCAGT GCGGGTTCCG CGGTGCTGAG CCCCTCGTCC 
AGCTGCAGTG AGAGCTGCAC GGCCGCCCTC GCTGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820600.1
CDS	84..3551
Translation	MFDCWRLILCQKTGSDDASSSRGSHEAEEEDSDPRVDVPDVIRLVPDPPEGPPLPADEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPARSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_010822298.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822298.2

ORF Insert Sequence:

ATGTTCGACT GTTGGAGGCT CATCCTTTGC CAGAAAACCG GAAGCGACGA TGCTTCTTCC 
TCGCGAGGGT CCCACGAGGC CGAAGAGGAG GACAGCGACC CTCGGGTGGA CGTCCCTGAC 
GTCATCAGGC TTGTTCCAGA CCCGCCGGAG GGGCCACCCC TGCCTGCAGA TGAGATCATG 
CACCACGACA TCAGCCCGCT CTGTGCCGCC GACATCCGGG ACCAGCTGCA GAAGCGCTTC 
GCTTACCTGT CAGGCGGGCG GGGCCAGGAC GGCAGCCCGG TCATCACCTT TCCCGACTAC 
CCGGCCTTCG GAGACGTCCC AGACCAGGAC TTCCAGAATG TCATGACCTA CCTCACCAGC 
ATCCCCAGCC TGCAGGACGC TGGCATCGGG TTCATCCTGG TCATAGACCG CAGGCAGGAC 
AGATGGACCT CGGTCAAGGC GTCCATCCTG AGAATAGCGG CATCCTTCCC CGCAAACCTG 
CAGCTCGTCC TCGTCCTGCG GCCAACCGGG TTCTTCCAGA GGACACTCTC CGACCTCGCC 
TTCAGGTTCA ACAGAGATGA CTTTAAGATG AAGGTGCCGG TCATAATGCT GAGCTCAGTC 
CCAGAACTAC ACGGCTACAT CGACAAGTCC CAGCTGACGG AGGACCTGGG TGGGACGCTG 
GCCTACTGCC ACTCCAGGTG GCTCCGCCAT CGCACAGCCA TCGAGAGCTT CGCCCTCCTG 
GTCAAGCAGA CGGCGCAGAT GCTGCAGACC TTTGGGACCG AGCTGGCCGA GACGGAGCTG 
CCCAATGACG TGCAGTCCAC GAGCTCCGTG CTCCTCGCCC ACACGGAGAA GAAGGACAGA 
GCCAAGGAGG ATATGAGGCT GGCGCTTGTC GAGGGGCGGC GCGTCCTGGA GAGCATCCAC 
GAGCCACTGG CCCGGAGCCC AGATCAGAGC CCCAACCAGG ACCAACTGGA CAGTCAGAGC 
ACCGTGCACA GGCTCCTGGC CCAGCTGAAC GAGACCGAGG CCGCCTTTGA CGAGTTCTGG 
GCAAAACATC AGCAAAAACT GGAGCAGTGC CTGCAGCTCC GGCATTTCGA GCAGGACTTC 
CGAGAGGTCA AATCGGCCCT GGACGTGCTG GCCCAGAAGG TCACGACTTT CACCGACGTG 
GGCAACAGCC TGGCACACGT GCAGCACCTC CTGAAGGACC TGACCAGCTT CGAGGAGAAG 
TCCAGTGCCG CCGTGCAGCG GGCCCGCACG CTGTCCCAGG AGGGTGAGCG GCTCATCGAG 
CACAAGCACT ATGCCGTGGA CTCCATCCGC CCCAAGTGTC ACGAGCTCCG CCACCTGTGC 
GACCAGTTCG CGGCCGACGT GGAGCGGAGG AGGGGGCTGC TGGGCAAGTC GCTGGAGCTG 
CACGGCCTCC TGGAGGCGTC CATGAAGTGG TGTGACGAGG GCATCTACCT GCTGGCATCG 
CAGCCTGTGG ACAAGTGCCA GTCCCAGGAT GGCGCAGAGG CCGCCCTCCA GGAGCTCGAG 
AGGTTTCTGG AGACGGGGGC AGAAAATAAG ATCCAGGAGC TCAGCAAGAT CTTCCAGGAC 
TACGAGCCCA TCCTCACCAA AGACCTGCTG GAGCATGTGC AGAGGGTCTT CCAGAAGCAG 
GAGAGCGTGG AGGAGATGTT CCACCGCAGG CAGGCCAGCC TCAAGAAGCT GGCGGCCAAG 
CAGACTCGGC CCGTGCAGCC CGTGGCCCCC CGGCCGGAAG CACTCACCAA GTCGCCCTGC 
CCCTCCCCAG GCATCCGGAG GGGTTCTGAG AACCACAGTT CCGAGGGCAG TGCACTCCGG 
AGGGGGCCCT ACAGGAGGGC CAAGAGTGAG GTGAGCGAGG GCCGGCAGGG CCGTGGCGGC 
TCCACGGGGG ACGAGGAGAG CCTGGCCGTC CTGCGGAGGC ATGTGATGAA GGAGCTTCTG 
GACACAGAGC GGGTCTACGT GGAGGAGCTG CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG 
ATGGACAACC CGTTAATGAC TCATCTCATC TCCACTGGCC TTCAGAACAA GAAGGACATC 
CTGTTTGGAA ACATGGAAGA AATTTACCAC TTTCATAACA GGATATTCCT GAGGGAGCTG 
GAGAACTACA CGGACTGCCC GGAGCTGGTT GGCAGGTGCT TTCTGGAGCG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCGCGCT CCGAGAGCCT GTGGAGACAG 
TGCTCCGACT GCCCCTTCTT CCAGGAGTGC CAGAAGAAGC TGGACCACAA GCTGAGCCTG 
GACTCCTACC TGCTGAAGCC AGTCCAGAGG ATCACCAAGT ACCAGCTGCT GCTGAAGGAG 
ATGCTTAAAT ACAGCAAGAG TTGCGAGGGG GCGGAGGACC TGCAGGAGGC CCTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC CGTGAACGAC TCCATGCACC TGATCGCCAT CACCGGCTAC 
GAGGGGAACC TGAGTGACCT GGGGAGGCTG CTCATGCAGG GCTCCTTCAG TGTGTGGACC 
GACCACAAGA GGGGCCACGC CAAGGTGAAG GACCTGGCCC GCTTCAAGCC CATGCAGCGC 
CACCTGTTCC TGCACGAGAA GGCCGTGCTC TTCTGTAAGC GGCGGGAGGA GAACGGCGAG 
GGCTACGAGA AGGCGCCCTC CTACAGCTAC AAGCAGTCCC TGAACATGAC GGCCGTCGGC 
ATCACGGAGA ACGTGAAAGG AGACGCCAAG AAGTTTGAGA TCTGGTACAA CGCCCGGGAG 
GAGGTGTACA TCATCCAGGC ACCCACTCCT GAAATCAAGG CCGCCTGGGT GAGCGAGATC 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCA TGCAGAGAGG CCAGCCAGCA TCGCGCCCTC 
GAGCAGTCCC AGAGCCTGCC CCTGCCCACA CCAGCTGGCA CCAGTCCCTC GAAAGCGAGC 
ACAAGAAACA TAAAAAAGTT AGAAGAAAGA AAAACGGACC CGCTGAGCCT GGAGGGATAC 
GGGGGGCCGA CACCACAGCA GAGGGCCCCC GAGAAGGGCA AAGGCTGGAG CAAAACGTCC 
CACCCATCAG AGACGCCTGA GGACAACGAT GGCTGGTCCA GCACCGAGGA GCCCGTCAAC 
TCCTCGGACG CGGAGGAGGA GGGCAGAGCG GGCCCCGGGA AGCTGGTCCC CGGGAGGTAC 
ACCGTCACCA GGCTGGACGA GAAGGGGGTC TCCGACGTGC TGGCCCTGAG AAGCGGGGAC 
GAGGTGGAGC TCGTGCAGGA GGGAGATGAG GGCCTCTGGT GCGTGCGGAA CCTGAGCTCC 
GGCGTCGAGG GCTGGGTGCC CGCCCGCAGC CTGTCCACTC TCCTCTGCCA GGGTGGCCCC 
GTGGGGTGCC TGAGCAGCCC AGAGTCCAGT GCGGGTTCCG CGGTGCTGAG CCCCTCGTCC 
AGCTGCAGTG AGAGCTGCAC GGCCGCCCTC GCTGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_010822299.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820601.1 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OBa46311
Clone ID Related Accession (Same CDS sequence)	XM_010822299.2 , XM_010810999.2
Accession Version	XM_010822299.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646112.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822299.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTGA GCCTCCTCTC TCTGCTGTCG CGGGACCTGT CCACGCTCTG GCTGCTCCTG 
AAGACCCGCA CAGATGAGAT CATGCACCAC GACATCAGCC CGCTCTGTGC CGCCGACATC 
CGGGACCAGC TGCAGAAGCG CTTCGCTTAC CTGTCAGGCG GGCGGGGCCA GGACGGCAGC 
CCGGTCATCA CCTTTCCCGA CTACCCGGCC TTCGGAGACG TCCCAGACCA GGACTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGGTTCATC 
CTGGTCATAG ACCGCAGGCA GGACAGATGG ACCTCGGTCA AGGCGTCCAT CCTGAGAATA 
GCGGCATCCT TCCCCGCAAA CCTGCAGCTC GTCCTCGTCC TGCGGCCAAC CGGGTTCTTC 
CAGAGGACAC TCTCCGACCT CGCCTTCAGG TTCAACAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC AGTCCCAGAA CTACACGGCT ACATCGACAA GTCCCAGCTG 
ACGGAGGACC TGGGTGGGAC GCTGGCCTAC TGCCACTCCA GGTGGCTCCG CCATCGCACA 
GCCATCGAGA GCTTCGCCCT CCTGGTCAAG CAGACGGCGC AGATGCTGCA GACCTTTGGG 
ACCGAGCTGG CCGAGACGGA GCTGCCCAAT GACGTGCAGT CCACGAGCTC CGTGCTCCTC 
GCCCACACGG AGAAGAAGGA CAGAGCCAAG GAGGATATGA GGCTGGCGCT TGTCGAGGGG 
CGGCGCGTCC TGGAGAGCAT CCACGAGCCA CTGGCCCGGA GCCCAGATCA GAGCCCCAAC 
CAGGACCAAC TGGACAGTCA GAGCACCGTG CACAGGCTCC TGGCCCAGCT GAACGAGACC 
GAGGCCGCCT TTGACGAGTT CTGGGCAAAA CATCAGCAAA AACTGGAGCA GTGCCTGCAG 
CTCCGGCATT TCGAGCAGGA CTTCCGAGAG GTCAAATCGG CCCTGGACGT GCTGGCCCAG 
AAGGTCACGA CTTTCACCGA CGTGGGCAAC AGCCTGGCAC ACGTGCAGCA CCTCCTGAAG 
GACCTGACCA GCTTCGAGGA GAAGTCCAGT GCCGCCGTGC AGCGGGCCCG CACGCTGTCC 
CAGGAGGGTG AGCGGCTCAT CGAGCACAAG CACTATGCCG TGGACTCCAT CCGCCCCAAG 
TGTCACGAGC TCCGCCACCT GTGCGACCAG TTCGCGGCCG ACGTGGAGCG GAGGAGGGGG 
CTGCTGGGCA AGTCGCTGGA GCTGCACGGC CTCCTGGAGG CGTCCATGAA GTGGTGTGAC 
GAGGGCATCT ACCTGCTGGC ATCGCAGCCT GTGGACAAGT GCCAGTCCCA GGATGGCGCA 
GAGGCCGCCC TCCAGGAGCT CGAGAGGTTT CTGGAGACGG GGGCAGAAAA TAAGATCCAG 
GAGCTCAGCA AGATCTTCCA GGACTACGAG CCCATCCTCA CCAAAGACCT GCTGGAGCAT 
GTGCAGAGGG TCTTCCAGAA GCAGGAGAGC GTGGAGGAGA TGTTCCACCG CAGGCAGGCC 
AGCCTCAAGA AGCTGGCGGC CAAGCAGACT CGGCCCGTGC AGCCCGTGGC CCCCCGGCCG 
GAAGCACTCA CCAAGTCGCC CTGCCCCTCC CCAGGCATCC GGAGGGGTTC TGAGAACCAC 
AGTTCCGAGG GCAGTGCACT CCGGAGGGGG CCCTACAGGA GGGCCAAGAG TGAGGTGAGC 
GAGGGCCGGC AGGGCCGTGG CGGCTCCACG GGGGACGAGG AGAGCCTGGC CGTCCTGCGG 
AGGCATGTGA TGAAGGAGCT TCTGGACACA GAGCGGGTCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAC AACCCGTTAA TGACTCATCT CATCTCCACT 
GGCCTTCAGA ACAAGAAGGA CATCCTGTTT GGAAACATGG AAGAAATTTA CCACTTTCAT 
AACAGGATAT TCCTGAGGGA GCTGGAGAAC TACACGGACT GCCCGGAGCT GGTTGGCAGG 
TGCTTTCTGG AGCGGATGGA GGAGTTCCAG ATCTACGAGA AGTACTGTCA GAACAAGCCG 
CGCTCCGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCCT TCTTCCAGGA GTGCCAGAAG 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTCCA GAGGATCACC 
AAGTACCAGC TGCTGCTGAA GGAGATGCTT AAATACAGCA AGAGTTGCGA GGGGGCGGAG 
GACCTGCAGG AGGCCCTGAG CTCCATCCTG GGCATCCTCA AGGCCGTGAA CGACTCCATG 
CACCTGATCG CCATCACCGG CTACGAGGGG AACCTGAGTG ACCTGGGGAG GCTGCTCATG 
CAGGGCTCCT TCAGTGTGTG GACCGACCAC AAGAGGGGCC ACGCCAAGGT GAAGGACCTG 
GCCCGCTTCA AGCCCATGCA GCGCCACCTG TTCCTGCACG AGAAGGCCGT GCTCTTCTGT 
AAGCGGCGGG AGGAGAACGG CGAGGGCTAC GAGAAGGCGC CCTCCTACAG CTACAAGCAG 
TCCCTGAACA TGACGGCCGT CGGCATCACG GAGAACGTGA AAGGAGACGC CAAGAAGTTT 
GAGATCTGGT ACAACGCCCG GGAGGAGGTG TACATCATCC AGGCACCCAC TCCTGAAATC 
AAGGCCGCCT GGGTGAGCGA GATCCGGAAG GTGCTGACCA GCCAGCTGCA GGCATGCAGA 
GAGGCCAGCC AGCATCGCGC CCTCGAGCAG TCCCAGAGCC TGCCCCTGCC CACACCAGCT 
GGCACCAGTC CCTCGAAAGC GAGCACAAGA AACATAAAAA AGTTAGAAGA AAGAAAAACG 
GACCCGCTGA GCCTGGAGGG ATACGGGGGG CCGACACCAC AGCAGAGGGC CCCCGAGAAG 
GGCAAAGGCT GGAGCAAAAC GTCCCACCCA TCAGAGACGC CTGAGGACAA CGATGGCTGG 
TCCAGCACCG AGGAGCCCGT CAACTCCTCG GACGCGGAGG AGGAGGGCAG AGCGGGCCCC 
GGGAAGCTGG TCCCCGGGAG GTACACCGTC ACCAGGCTGG ACGAGAAGGG GGTCTCCGAC 
GTGCTGGCCC TGAGAAGCGG GGACGAGGTG GAGCTCGTGC AGGAGGGAGA TGAGGGCCTC 
TGGTGCGTGC GGAACCTGAG CTCCGGCGTC GAGGGCTGGG TGCCCGCCCG CAGCCTGTCC 
ACTCTCCTCT GCCAGGGTGG CCCCGTGGGG TGCCTGAGCA GCCCAGAGTC CAGTGCGGGT 
TCCGCGGTGC TGAGCCCCTC GTCCAGCTGC AGTGAGAGCT GCACGGCCGC CCTCGCTGAC 
CTGCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820601.1
CDS	22..3393
Translation	MTLSLLSLLSRDLSTLWLLLKTRTDEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPARSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_010822299.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822299.2

ORF Insert Sequence:

ATGACCCTGA GCCTCCTCTC TCTGCTGTCG CGGGACCTGT CCACGCTCTG GCTGCTCCTG 
AAGACCCGCA CAGATGAGAT CATGCACCAC GACATCAGCC CGCTCTGTGC CGCCGACATC 
CGGGACCAGC TGCAGAAGCG CTTCGCTTAC CTGTCAGGCG GGCGGGGCCA GGACGGCAGC 
CCGGTCATCA CCTTTCCCGA CTACCCGGCC TTCGGAGACG TCCCAGACCA GGACTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGGTTCATC 
CTGGTCATAG ACCGCAGGCA GGACAGATGG ACCTCGGTCA AGGCGTCCAT CCTGAGAATA 
GCGGCATCCT TCCCCGCAAA CCTGCAGCTC GTCCTCGTCC TGCGGCCAAC CGGGTTCTTC 
CAGAGGACAC TCTCCGACCT CGCCTTCAGG TTCAACAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC AGTCCCAGAA CTACACGGCT ACATCGACAA GTCCCAGCTG 
ACGGAGGACC TGGGTGGGAC GCTGGCCTAC TGCCACTCCA GGTGGCTCCG CCATCGCACA 
GCCATCGAGA GCTTCGCCCT CCTGGTCAAG CAGACGGCGC AGATGCTGCA GACCTTTGGG 
ACCGAGCTGG CCGAGACGGA GCTGCCCAAT GACGTGCAGT CCACGAGCTC CGTGCTCCTC 
GCCCACACGG AGAAGAAGGA CAGAGCCAAG GAGGATATGA GGCTGGCGCT TGTCGAGGGG 
CGGCGCGTCC TGGAGAGCAT CCACGAGCCA CTGGCCCGGA GCCCAGATCA GAGCCCCAAC 
CAGGACCAAC TGGACAGTCA GAGCACCGTG CACAGGCTCC TGGCCCAGCT GAACGAGACC 
GAGGCCGCCT TTGACGAGTT CTGGGCAAAA CATCAGCAAA AACTGGAGCA GTGCCTGCAG 
CTCCGGCATT TCGAGCAGGA CTTCCGAGAG GTCAAATCGG CCCTGGACGT GCTGGCCCAG 
AAGGTCACGA CTTTCACCGA CGTGGGCAAC AGCCTGGCAC ACGTGCAGCA CCTCCTGAAG 
GACCTGACCA GCTTCGAGGA GAAGTCCAGT GCCGCCGTGC AGCGGGCCCG CACGCTGTCC 
CAGGAGGGTG AGCGGCTCAT CGAGCACAAG CACTATGCCG TGGACTCCAT CCGCCCCAAG 
TGTCACGAGC TCCGCCACCT GTGCGACCAG TTCGCGGCCG ACGTGGAGCG GAGGAGGGGG 
CTGCTGGGCA AGTCGCTGGA GCTGCACGGC CTCCTGGAGG CGTCCATGAA GTGGTGTGAC 
GAGGGCATCT ACCTGCTGGC ATCGCAGCCT GTGGACAAGT GCCAGTCCCA GGATGGCGCA 
GAGGCCGCCC TCCAGGAGCT CGAGAGGTTT CTGGAGACGG GGGCAGAAAA TAAGATCCAG 
GAGCTCAGCA AGATCTTCCA GGACTACGAG CCCATCCTCA CCAAAGACCT GCTGGAGCAT 
GTGCAGAGGG TCTTCCAGAA GCAGGAGAGC GTGGAGGAGA TGTTCCACCG CAGGCAGGCC 
AGCCTCAAGA AGCTGGCGGC CAAGCAGACT CGGCCCGTGC AGCCCGTGGC CCCCCGGCCG 
GAAGCACTCA CCAAGTCGCC CTGCCCCTCC CCAGGCATCC GGAGGGGTTC TGAGAACCAC 
AGTTCCGAGG GCAGTGCACT CCGGAGGGGG CCCTACAGGA GGGCCAAGAG TGAGGTGAGC 
GAGGGCCGGC AGGGCCGTGG CGGCTCCACG GGGGACGAGG AGAGCCTGGC CGTCCTGCGG 
AGGCATGTGA TGAAGGAGCT TCTGGACACA GAGCGGGTCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAC AACCCGTTAA TGACTCATCT CATCTCCACT 
GGCCTTCAGA ACAAGAAGGA CATCCTGTTT GGAAACATGG AAGAAATTTA CCACTTTCAT 
AACAGGATAT TCCTGAGGGA GCTGGAGAAC TACACGGACT GCCCGGAGCT GGTTGGCAGG 
TGCTTTCTGG AGCGGATGGA GGAGTTCCAG ATCTACGAGA AGTACTGTCA GAACAAGCCG 
CGCTCCGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCCT TCTTCCAGGA GTGCCAGAAG 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTCCA GAGGATCACC 
AAGTACCAGC TGCTGCTGAA GGAGATGCTT AAATACAGCA AGAGTTGCGA GGGGGCGGAG 
GACCTGCAGG AGGCCCTGAG CTCCATCCTG GGCATCCTCA AGGCCGTGAA CGACTCCATG 
CACCTGATCG CCATCACCGG CTACGAGGGG AACCTGAGTG ACCTGGGGAG GCTGCTCATG 
CAGGGCTCCT TCAGTGTGTG GACCGACCAC AAGAGGGGCC ACGCCAAGGT GAAGGACCTG 
GCCCGCTTCA AGCCCATGCA GCGCCACCTG TTCCTGCACG AGAAGGCCGT GCTCTTCTGT 
AAGCGGCGGG AGGAGAACGG CGAGGGCTAC GAGAAGGCGC CCTCCTACAG CTACAAGCAG 
TCCCTGAACA TGACGGCCGT CGGCATCACG GAGAACGTGA AAGGAGACGC CAAGAAGTTT 
GAGATCTGGT ACAACGCCCG GGAGGAGGTG TACATCATCC AGGCACCCAC TCCTGAAATC 
AAGGCCGCCT GGGTGAGCGA GATCCGGAAG GTGCTGACCA GCCAGCTGCA GGCATGCAGA 
GAGGCCAGCC AGCATCGCGC CCTCGAGCAG TCCCAGAGCC TGCCCCTGCC CACACCAGCT 
GGCACCAGTC CCTCGAAAGC GAGCACAAGA AACATAAAAA AGTTAGAAGA AAGAAAAACG 
GACCCGCTGA GCCTGGAGGG ATACGGGGGG CCGACACCAC AGCAGAGGGC CCCCGAGAAG 
GGCAAAGGCT GGAGCAAAAC GTCCCACCCA TCAGAGACGC CTGAGGACAA CGATGGCTGG 
TCCAGCACCG AGGAGCCCGT CAACTCCTCG GACGCGGAGG AGGAGGGCAG AGCGGGCCCC 
GGGAAGCTGG TCCCCGGGAG GTACACCGTC ACCAGGCTGG ACGAGAAGGG GGTCTCCGAC 
GTGCTGGCCC TGAGAAGCGG GGACGAGGTG GAGCTCGTGC AGGAGGGAGA TGAGGGCCTC 
TGGTGCGTGC GGAACCTGAG CTCCGGCGTC GAGGGCTGGG TGCCCGCCCG CAGCCTGTCC 
ACTCTCCTCT GCCAGGGTGG CCCCGTGGGG TGCCTGAGCA GCCCAGAGTC CAGTGCGGGT 
TCCGCGGTGC TGAGCCCCTC GTCCAGCTGC AGTGAGAGCT GCACGGCCGC CCTCGCTGAC 
CTGCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_010810999.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010809301.1 Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OBa46311
Clone ID Related Accession (Same CDS sequence)	XM_010822299.2 , XM_010810999.2
Accession Version	XM_010810999.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanine nucleotide exchange factor DBS isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810999.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTGA GCCTCCTCTC TCTGCTGTCG CGGGACCTGT CCACGCTCTG GCTGCTCCTG 
AAGACCCGCA CAGATGAGAT CATGCACCAC GACATCAGCC CGCTCTGTGC CGCCGACATC 
CGGGACCAGC TGCAGAAGCG CTTCGCTTAC CTGTCAGGCG GGCGGGGCCA GGACGGCAGC 
CCGGTCATCA CCTTTCCCGA CTACCCGGCC TTCGGAGACG TCCCAGACCA GGACTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGGTTCATC 
CTGGTCATAG ACCGCAGGCA GGACAGATGG ACCTCGGTCA AGGCGTCCAT CCTGAGAATA 
GCGGCATCCT TCCCCGCAAA CCTGCAGCTC GTCCTCGTCC TGCGGCCAAC CGGGTTCTTC 
CAGAGGACAC TCTCCGACCT CGCCTTCAGG TTCAACAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC AGTCCCAGAA CTACACGGCT ACATCGACAA GTCCCAGCTG 
ACGGAGGACC TGGGTGGGAC GCTGGCCTAC TGCCACTCCA GGTGGCTCCG CCATCGCACA 
GCCATCGAGA GCTTCGCCCT CCTGGTCAAG CAGACGGCGC AGATGCTGCA GACCTTTGGG 
ACCGAGCTGG CCGAGACGGA GCTGCCCAAT GACGTGCAGT CCACGAGCTC CGTGCTCCTC 
GCCCACACGG AGAAGAAGGA CAGAGCCAAG GAGGATATGA GGCTGGCGCT TGTCGAGGGG 
CGGCGCGTCC TGGAGAGCAT CCACGAGCCA CTGGCCCGGA GCCCAGATCA GAGCCCCAAC 
CAGGACCAAC TGGACAGTCA GAGCACCGTG CACAGGCTCC TGGCCCAGCT GAACGAGACC 
GAGGCCGCCT TTGACGAGTT CTGGGCAAAA CATCAGCAAA AACTGGAGCA GTGCCTGCAG 
CTCCGGCATT TCGAGCAGGA CTTCCGAGAG GTCAAATCGG CCCTGGACGT GCTGGCCCAG 
AAGGTCACGA CTTTCACCGA CGTGGGCAAC AGCCTGGCAC ACGTGCAGCA CCTCCTGAAG 
GACCTGACCA GCTTCGAGGA GAAGTCCAGT GCCGCCGTGC AGCGGGCCCG CACGCTGTCC 
CAGGAGGGTG AGCGGCTCAT CGAGCACAAG CACTATGCCG TGGACTCCAT CCGCCCCAAG 
TGTCACGAGC TCCGCCACCT GTGCGACCAG TTCGCGGCCG ACGTGGAGCG GAGGAGGGGG 
CTGCTGGGCA AGTCGCTGGA GCTGCACGGC CTCCTGGAGG CGTCCATGAA GTGGTGTGAC 
GAGGGCATCT ACCTGCTGGC ATCGCAGCCT GTGGACAAGT GCCAGTCCCA GGATGGCGCA 
GAGGCCGCCC TCCAGGAGCT CGAGAGGTTT CTGGAGACGG GGGCAGAAAA TAAGATCCAG 
GAGCTCAGCA AGATCTTCCA GGACTACGAG CCCATCCTCA CCAAAGACCT GCTGGAGCAT 
GTGCAGAGGG TCTTCCAGAA GCAGGAGAGC GTGGAGGAGA TGTTCCACCG CAGGCAGGCC 
AGCCTCAAGA AGCTGGCGGC CAAGCAGACT CGGCCCGTGC AGCCCGTGGC CCCCCGGCCG 
GAAGCACTCA CCAAGTCGCC CTGCCCCTCC CCAGGCATCC GGAGGGGTTC TGAGAACCAC 
AGTTCCGAGG GCAGTGCACT CCGGAGGGGG CCCTACAGGA GGGCCAAGAG TGAGGTGAGC 
GAGGGCCGGC AGGGCCGTGG CGGCTCCACG GGGGACGAGG AGAGCCTGGC CGTCCTGCGG 
AGGCATGTGA TGAAGGAGCT TCTGGACACA GAGCGGGTCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAC AACCCGTTAA TGACTCATCT CATCTCCACT 
GGCCTTCAGA ACAAGAAGGA CATCCTGTTT GGAAACATGG AAGAAATTTA CCACTTTCAT 
AACAGGATAT TCCTGAGGGA GCTGGAGAAC TACACGGACT GCCCGGAGCT GGTTGGCAGG 
TGCTTTCTGG AGCGGATGGA GGAGTTCCAG ATCTACGAGA AGTACTGTCA GAACAAGCCG 
CGCTCCGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCCT TCTTCCAGGA GTGCCAGAAG 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTCCA GAGGATCACC 
AAGTACCAGC TGCTGCTGAA GGAGATGCTT AAATACAGCA AGAGTTGCGA GGGGGCGGAG 
GACCTGCAGG AGGCCCTGAG CTCCATCCTG GGCATCCTCA AGGCCGTGAA CGACTCCATG 
CACCTGATCG CCATCACCGG CTACGAGGGG AACCTGAGTG ACCTGGGGAG GCTGCTCATG 
CAGGGCTCCT TCAGTGTGTG GACCGACCAC AAGAGGGGCC ACGCCAAGGT GAAGGACCTG 
GCCCGCTTCA AGCCCATGCA GCGCCACCTG TTCCTGCACG AGAAGGCCGT GCTCTTCTGT 
AAGCGGCGGG AGGAGAACGG CGAGGGCTAC GAGAAGGCGC CCTCCTACAG CTACAAGCAG 
TCCCTGAACA TGACGGCCGT CGGCATCACG GAGAACGTGA AAGGAGACGC CAAGAAGTTT 
GAGATCTGGT ACAACGCCCG GGAGGAGGTG TACATCATCC AGGCACCCAC TCCTGAAATC 
AAGGCCGCCT GGGTGAGCGA GATCCGGAAG GTGCTGACCA GCCAGCTGCA GGCATGCAGA 
GAGGCCAGCC AGCATCGCGC CCTCGAGCAG TCCCAGAGCC TGCCCCTGCC CACACCAGCT 
GGCACCAGTC CCTCGAAAGC GAGCACAAGA AACATAAAAA AGTTAGAAGA AAGAAAAACG 
GACCCGCTGA GCCTGGAGGG ATACGGGGGG CCGACACCAC AGCAGAGGGC CCCCGAGAAG 
GGCAAAGGCT GGAGCAAAAC GTCCCACCCA TCAGAGACGC CTGAGGACAA CGATGGCTGG 
TCCAGCACCG AGGAGCCCGT CAACTCCTCG GACGCGGAGG AGGAGGGCAG AGCGGGCCCC 
GGGAAGCTGG TCCCCGGGAG GTACACCGTC ACCAGGCTGG ACGAGAAGGG GGTCTCCGAC 
GTGCTGGCCC TGAGAAGCGG GGACGAGGTG GAGCTCGTGC AGGAGGGAGA TGAGGGCCTC 
TGGTGCGTGC GGAACCTGAG CTCCGGCGTC GAGGGCTGGG TGCCCGCCCG CAGCCTGTCC 
ACTCTCCTCT GCCAGGGTGG CCCCGTGGGG TGCCTGAGCA GCCCAGAGTC CAGTGCGGGT 
TCCGCGGTGC TGAGCCCCTC GTCCAGCTGC AGTGAGAGCT GCACGGCCGC CCTCGCTGAC 
CTGCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010809301.1
CDS	22..3393
Translation	MTLSLLSLLSRDLSTLWLLLKTRTDEIMHHDISPLCAADIRDQLQKRFAYLSGGRGQDGSPVITFPDYPAFGDVPDQDFQNVMTYLTSIPSLQDAGIGFILVIDRRQDRWTSVKASILRIAASFPANLQLVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVIMLSSVPELHGYIDKSQLTEDLGGTLAYCHSRWLRHRTAIESFALLVKQTAQMLQTFGTELAETELPNDVQSTSSVLLAHTEKKDRAKEDMRLALVEGRRVLESIHEPLARSPDQSPNQDQLDSQSTVHRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKSALDVLAQKVTTFTDVGNSLAHVQHLLKDLTSFEEKSSAAVQRARTLSQEGERLIEHKHYAVDSIRPKCHELRHLCDQFAADVERRRGLLGKSLELHGLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQELERFLETGAENKIQELSKIFQDYEPILTKDLLEHVQRVFQKQESVEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGIRRGSENHSSEGSALRRGPYRRAKSEVSEGRQGRGGSTGDEESLAVLRRHVMKELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDILFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYEGNLSDLGRLLMQGSFSVWTDHKRGHAKVKDLARFKPMQRHLFLHEKAVLFCKRREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSQSLPLPTPAGTSPSKASTRNIKKLEERKTDPLSLEGYGGPTPQQRAPEKGKGWSKTSHPSETPEDNDGWSSTEEPVNSSDAEEEGRAGPGKLVPGRYTVTRLDEKGVSDVLALRSGDEVELVQEGDEGLWCVRNLSSGVEGWVPARSLSTLLCQGGPVGCLSSPESSAGSAVLSPSSSCSESCTAALADLQG

Target ORF information:

RefSeq Version	XM_010810999.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810999.2

ORF Insert Sequence:

ATGACCCTGA GCCTCCTCTC TCTGCTGTCG CGGGACCTGT CCACGCTCTG GCTGCTCCTG 
AAGACCCGCA CAGATGAGAT CATGCACCAC GACATCAGCC CGCTCTGTGC CGCCGACATC 
CGGGACCAGC TGCAGAAGCG CTTCGCTTAC CTGTCAGGCG GGCGGGGCCA GGACGGCAGC 
CCGGTCATCA CCTTTCCCGA CTACCCGGCC TTCGGAGACG TCCCAGACCA GGACTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGGTTCATC 
CTGGTCATAG ACCGCAGGCA GGACAGATGG ACCTCGGTCA AGGCGTCCAT CCTGAGAATA 
GCGGCATCCT TCCCCGCAAA CCTGCAGCTC GTCCTCGTCC TGCGGCCAAC CGGGTTCTTC 
CAGAGGACAC TCTCCGACCT CGCCTTCAGG TTCAACAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC AGTCCCAGAA CTACACGGCT ACATCGACAA GTCCCAGCTG 
ACGGAGGACC TGGGTGGGAC GCTGGCCTAC TGCCACTCCA GGTGGCTCCG CCATCGCACA 
GCCATCGAGA GCTTCGCCCT CCTGGTCAAG CAGACGGCGC AGATGCTGCA GACCTTTGGG 
ACCGAGCTGG CCGAGACGGA GCTGCCCAAT GACGTGCAGT CCACGAGCTC CGTGCTCCTC 
GCCCACACGG AGAAGAAGGA CAGAGCCAAG GAGGATATGA GGCTGGCGCT TGTCGAGGGG 
CGGCGCGTCC TGGAGAGCAT CCACGAGCCA CTGGCCCGGA GCCCAGATCA GAGCCCCAAC 
CAGGACCAAC TGGACAGTCA GAGCACCGTG CACAGGCTCC TGGCCCAGCT GAACGAGACC 
GAGGCCGCCT TTGACGAGTT CTGGGCAAAA CATCAGCAAA AACTGGAGCA GTGCCTGCAG 
CTCCGGCATT TCGAGCAGGA CTTCCGAGAG GTCAAATCGG CCCTGGACGT GCTGGCCCAG 
AAGGTCACGA CTTTCACCGA CGTGGGCAAC AGCCTGGCAC ACGTGCAGCA CCTCCTGAAG 
GACCTGACCA GCTTCGAGGA GAAGTCCAGT GCCGCCGTGC AGCGGGCCCG CACGCTGTCC 
CAGGAGGGTG AGCGGCTCAT CGAGCACAAG CACTATGCCG TGGACTCCAT CCGCCCCAAG 
TGTCACGAGC TCCGCCACCT GTGCGACCAG TTCGCGGCCG ACGTGGAGCG GAGGAGGGGG 
CTGCTGGGCA AGTCGCTGGA GCTGCACGGC CTCCTGGAGG CGTCCATGAA GTGGTGTGAC 
GAGGGCATCT ACCTGCTGGC ATCGCAGCCT GTGGACAAGT GCCAGTCCCA GGATGGCGCA 
GAGGCCGCCC TCCAGGAGCT CGAGAGGTTT CTGGAGACGG GGGCAGAAAA TAAGATCCAG 
GAGCTCAGCA AGATCTTCCA GGACTACGAG CCCATCCTCA CCAAAGACCT GCTGGAGCAT 
GTGCAGAGGG TCTTCCAGAA GCAGGAGAGC GTGGAGGAGA TGTTCCACCG CAGGCAGGCC 
AGCCTCAAGA AGCTGGCGGC CAAGCAGACT CGGCCCGTGC AGCCCGTGGC CCCCCGGCCG 
GAAGCACTCA CCAAGTCGCC CTGCCCCTCC CCAGGCATCC GGAGGGGTTC TGAGAACCAC 
AGTTCCGAGG GCAGTGCACT CCGGAGGGGG CCCTACAGGA GGGCCAAGAG TGAGGTGAGC 
GAGGGCCGGC AGGGCCGTGG CGGCTCCACG GGGGACGAGG AGAGCCTGGC CGTCCTGCGG 
AGGCATGTGA TGAAGGAGCT TCTGGACACA GAGCGGGTCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAC AACCCGTTAA TGACTCATCT CATCTCCACT 
GGCCTTCAGA ACAAGAAGGA CATCCTGTTT GGAAACATGG AAGAAATTTA CCACTTTCAT 
AACAGGATAT TCCTGAGGGA GCTGGAGAAC TACACGGACT GCCCGGAGCT GGTTGGCAGG 
TGCTTTCTGG AGCGGATGGA GGAGTTCCAG ATCTACGAGA AGTACTGTCA GAACAAGCCG 
CGCTCCGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCCT TCTTCCAGGA GTGCCAGAAG 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTCCA GAGGATCACC 
AAGTACCAGC TGCTGCTGAA GGAGATGCTT AAATACAGCA AGAGTTGCGA GGGGGCGGAG 
GACCTGCAGG AGGCCCTGAG CTCCATCCTG GGCATCCTCA AGGCCGTGAA CGACTCCATG 
CACCTGATCG CCATCACCGG CTACGAGGGG AACCTGAGTG ACCTGGGGAG GCTGCTCATG 
CAGGGCTCCT TCAGTGTGTG GACCGACCAC AAGAGGGGCC ACGCCAAGGT GAAGGACCTG 
GCCCGCTTCA AGCCCATGCA GCGCCACCTG TTCCTGCACG AGAAGGCCGT GCTCTTCTGT 
AAGCGGCGGG AGGAGAACGG CGAGGGCTAC GAGAAGGCGC CCTCCTACAG CTACAAGCAG 
TCCCTGAACA TGACGGCCGT CGGCATCACG GAGAACGTGA AAGGAGACGC CAAGAAGTTT 
GAGATCTGGT ACAACGCCCG GGAGGAGGTG TACATCATCC AGGCACCCAC TCCTGAAATC 
AAGGCCGCCT GGGTGAGCGA GATCCGGAAG GTGCTGACCA GCCAGCTGCA GGCATGCAGA 
GAGGCCAGCC AGCATCGCGC CCTCGAGCAG TCCCAGAGCC TGCCCCTGCC CACACCAGCT 
GGCACCAGTC CCTCGAAAGC GAGCACAAGA AACATAAAAA AGTTAGAAGA AAGAAAAACG 
GACCCGCTGA GCCTGGAGGG ATACGGGGGG CCGACACCAC AGCAGAGGGC CCCCGAGAAG 
GGCAAAGGCT GGAGCAAAAC GTCCCACCCA TCAGAGACGC CTGAGGACAA CGATGGCTGG 
TCCAGCACCG AGGAGCCCGT CAACTCCTCG GACGCGGAGG AGGAGGGCAG AGCGGGCCCC 
GGGAAGCTGG TCCCCGGGAG GTACACCGTC ACCAGGCTGG ACGAGAAGGG GGTCTCCGAC 
GTGCTGGCCC TGAGAAGCGG GGACGAGGTG GAGCTCGTGC AGGAGGGAGA TGAGGGCCTC 
TGGTGCGTGC GGAACCTGAG CTCCGGCGTC GAGGGCTGGG TGCCCGCCCG CAGCCTGTCC 
ACTCTCCTCT GCCAGGGTGG CCCCGTGGGG TGCCTGAGCA GCCCAGAGTC CAGTGCGGGT 
TCCGCGGTGC TGAGCCCCTC GTCCAGCTGC AGTGAGAGCT GCACGGCCGC CCTCGCTGAC 
CTGCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MCF2L
Entrez Gene ID	505595
Full Name	MCF.2 cell line derived transforming sequence like
General protein information	Preferred Names MCF.2 cell line derived transforming sequence like Names guanine nucleotide exchange factor DBS
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Xenopus tropicalis (tropical clawed frog)	LOC100497730	XP_002933052.2
Homo sapiens (human)	MCF2L	NP_001106203.2
Pan troglodytes (chimpanzee)	MCF2L	XP_003314263.1
	MCF2L	XP_001103476.2
Mus musculus (house mouse)	Mcf2l	NP_835177.2
Rattus norvegicus (Norway rat)	Mcf2l	NP_446403.2
Canis lupus familiaris (dog)	MCF2L	XP_005634173.1
Gallus gallus (chicken)	MCF2L	NP_001238992.1
Danio rerio (zebrafish)	mcf2la	XP_005171065.1
Bos taurus (cattle)	MCF2L	XP_005214085.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MCF2L cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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