Mcf2l cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mcf2l
Entrez Gene ID	117020
Full Name	MCF.2 cell line derived transforming sequence-like
Synonyms	Ost,mcf 2l
General protein information	Preferred Names MCF.2 cell line derived transforming sequence-like Names guanine nucleotide exchange factor DBS DBL's big sister MCF2-transforming sequence-like protein OST oncogene mcf.2 transforming sequence-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16q12.5
Summary	oncogene that can activate small GTP-binding proteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006253416.3	XP_006253478.1	guanine nucleotide exchange factor DBS isoform X2
XM_017599979.1	XP_017455468.1	guanine nucleotide exchange factor DBS isoform X6
XM_006253421.3	XP_006253483.1	guanine nucleotide exchange factor DBS isoform X3
XM_008771381.2	XP_008769603.1	guanine nucleotide exchange factor DBS isoform X1
XM_006253423.3	XP_006253485.1	guanine nucleotide exchange factor DBS isoform X4
XM_006253424.3	XP_006253486.1	guanine nucleotide exchange factor DBS isoform X5
NM_053951.2	NP_446403.2	guanine nucleotide exchange factor DBS

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100497730	XP_002933052.2
Homo sapiens (human)	MCF2L	NP_001106203.2
Pan troglodytes (chimpanzee)	MCF2L	XP_003314263.1
	MCF2L	XP_001103476.2
Mus musculus (house mouse)	Mcf2l	NP_835177.2
Rattus norvegicus (Norway rat)	Mcf2l	NP_446403.2
Canis lupus familiaris (dog)	MCF2L	XP_005634173.1
Gallus gallus (chicken)	MCF2L	NP_001238992.1
Danio rerio (zebrafish)	mcf2la	XP_005171065.1
Bos taurus (cattle)	MCF2L	XP_005214085.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
B?hm D, Keller K, Pieter J, Boehm N, Wolters D, Siggelkow W, Lebrecht A, Schmidt M, K?lbl H, Pfeiffer N, Grus FH
Oncology reports28(2)429-38(2012 Aug)

Ccpg1, a novel scaffold protein that regulates the activity of the Rho guanine nucleotide exchange factor Dbs.
Kostenko EV, Olabisi OO, Sahay S, Rodriguez PL, Whitehead IP
Molecular and cellular biology26(23)8964-75(2006 Dec)

Role of the Sec14-like domain of Dbl family exchange factors in the regulation of Rho family GTPases in different subcellular sites.
Ueda S, Kataoka T, Satoh T
Cellular signalling16(8)899-906(2004 Aug)

Multifunctional roles for the PH domain of Dbs in regulating Rho GTPase activation.
Rossman KL, Cheng L, Mahon GM, Rojas RJ, Snyder JT, Whitehead IP, Sondek J
The Journal of biological chemistry278(20)18393-400(2003 May)

A novel oncogene, ost, encodes a guanine nucleotide exchange factor that potentially links Rho and Rac signaling pathways.
Horii Y, Beeler JF, Sakaguchi K, Tachibana M, Miki T
The EMBO journal13(20)4776-86(1994 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mcf2l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mcf2l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa44081	XM_008771381.2 Latest version!	Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
ORa58741	XM_017599979.1 Latest version!	Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa11158	NM_053951.2 Latest version!	Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa37550	XM_006253416.3 Latest version!	Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa37555	XM_006253421.3 Latest version!	Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa37557	XM_006253423.3 Latest version!	Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa37558	XM_006253424.3 Latest version!	Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mcf2l ( XM_008771381.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769603.1 Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa44081
Clone ID Related Accession (Same CDS sequence)	XM_008771381.2
Accession Version	XM_008771381.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008771381.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGGT GTGCCCTGGC AATCTACCGT TTGCAGTGGC ATTTAGAGTT ACCTACAGGT 
GGCTACTCAC GAAACGGAAT GTCAAGCAAG CAGTGCGAGT GGAGAAGTGG AGCACCCTCA 
GGTCAGATAA GAACAGAGGC CAGAAAAGCT TATGGAACGT GCATGAAGTC AAAGGGTTCC 
CACGAAGCAG TCTTGGGAGA GCCTGGTCAC CTGAGACTTC GGAGTGGACA CAATTTCCCT 
GCAGTCCAGC TGAGAAGTCT GCAACGGTCG CCTCAGTCCC CAGCGCCACC GCCCCTCCCC 
CACCGTGGCT CCCGCTCCCG CCTTCCCTTC TCCTCCCTCC CTGTCTCAGT GGCCCCAGAC 
CGACCGGCGG GCGGAGCCTG GGAGCGTGGC ACCTGCGAGC AAGTGAGCTC GCTGGACGAG 
GAGAAGACGG TGCCCCGCAC AGGCAGCCTC ATGTCCAACT GCTGGTGTTT CATCTTCTGC 
AAGGAACGAG TTAGAAGCAA TTCTTCATCG CCACAGCACG ATGGGACCTC GAGGGAGGAG 
GCTGATCACC AGGTGGACGT GTCTGATGGC ATCAGGCTGG TTCCAGACAA AGCAGAGGCC 
ACGATGGCCA CAGCCTCAGA TGAAATCATG CACCAGGACA TCGTCCCACT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCAGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATCGGG 
TTCATCTTGG TCATAGACCG AAGACAGGAC AAATGGACTT CTGTGAAGGC GTCAGTCCTG 
CGAATAGCCG CATCATTCCC AGCCAACCTG CAGCTCGTCC TCGTTCTCCG ACCCACGGGC 
TTCTTCCAGA GGACTCTCTC GGACATAGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTACCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ATGGCTACAT TGACAAGTCA 
CAGCTGACCG AGGACCTTGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCGA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCCGA GACTGAGCTG CCCAACGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCTAAA GTGAAGGAGG ATCTGCAGCT GGCGCTGACG 
GAGGGGAACA GCATCCTTGA GAGCCTCAGG GAGCCGCTGG CTGAGAGCAT AGTCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACTGTGAAGA GGCTCCTGAC CCAGCTGAAT 
GAGACCGAGG CTGCCTTTGA CGAGTTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTACAGCTGC GACATTTTGA ACAAGGTTTC CGGGAGGTCA AAACCGCCTT GGATTCCATG 
TCCCAGAAGA TAGCCGCGTT CACAGATGTT GGCAACAGCC TGGCCCATGT GCAGCACCTC 
CTGAAGGACT TGACCACCTT TGAGGAGAAG TCCAGCGTGG CCGTGGACAA GGCCAGAGCC 
CTGTCCCTGG AGGGGCAGCA GCTGATTGAG AACCGGCACT ATGCTGTAGA CTCTATCCAT 
CCGAAGTGTG AGGAACTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGACCTTC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GCATCTTCCT GCTGGCCTCT CAGCCTGTGG ACAAATGCCA ATCTCAGGAT 
GGTGCGGAGG CAGCCCTGCA GGAGATCGAG AAGTTTCTGG AGACCGGTGC AGAAAACAAG 
ATTCAGGAGC TCAATAAGAT TTACAAGGAA TATGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAGAGCACCG AGGAGATGTT CCACCGCAGG 
CAGGCTAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTAACCAA GTCCCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCCCTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAGC CCCGGCAGGG CCGCACCAGC TCCACAGGAG AAGAGGAGGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAACTT CTGGACACGG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCTGCA GAGATGGACA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CACTTCCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATCGACTG CCCAGAGCTG 
GTGGGGAGAT GCTTTCTGGA GAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCCGAGAG CCTGTGGAGA CAGTGCTCTG ACTGTCCCTT CTTCCAGGAG 
TGCCAGAAGA AGCTGGACCA CAAGCTGAGC CTCGACTCCT ACCTGCTGAA GCCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCCGAGG ACCTGCAAGA GGCACTGAGC TCCATCCTGG GCATCCTCAA GGCAGTGAAC 
GACTCCATGC ACCTCATAGC CATCACTGGC TATGATGGAA ACCTTGGGGA CCTGGGGAAG 
CTGCTGATGC AAGGCTCCTT CAGCGTGTGG ACGGACCACA AGAAGGGCCA CACCAAGGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTCT TCCTTCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGGTACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACTGCTGTT GGCATCACAG AGAATGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ACATCATTCA GGCACCAACT 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAACTGCAG 
GCCTGCCGGG AAGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCCTTGCCC 
ACGCCAGCCA GCACCAGTCC CACAAAAGGG AGCACAAGAA ATGTCAAGAA GCTCGAAGAT 
CGGAAGACGG ACCCGCTCTG CCTGGAAGGC TGCGTGAGCT CATCATTACC AAAGCCCCCC 
GAGAAGGGCA AAGACGATGC GGTCCCTAGT TCTACCTCTG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC CTCAAAGGTC AGAAAGCCTC TCCGACCAGT 
CCTGACAAAA AAGCTAAGCG GCATGAAGTA AAGAGCGACC CGACTCCCTT TGGTTTGCGA 
GGCTGGAGCA AAACATCCCA CTCACTGGAG GCCCCTGAGG AGGACGGAGG CTGGTCCAGT 
GCTGAGGAGC TCATCAATTC ATCCGATGCT GAGGAAGACG GAGGAGTGGG CCCTAGGAAG 
CTGGTTCCAG GTAAATACAC AGTGCTGATG GATGGTGAGA AAGGGGGCTC TGACACCCTT 
GCCATGAGGA GTGGAGATAT GGTGGAGGTT GTGGAAGAAG GAACTGAGGG CCTCTGGTAT 
GTTCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT AGCCACCCTC 
CTGGGCAAAT CCAGCTCAGC CCAGTGTCTG AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC 
GTGCTGAGCA ACTCATCCAG CTGCAGCGAG GGCTACCCTA CTCCCTTCTC CGAGCTCCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769603.1
CDS	45..4130
Translation	MFRCALAIYRLQWHLELPTGGYSRNGMSSKQCEWRSGAPSGQIRTEARKAYGTCMKSKGSHEAVLGEPGHLRLRSGHNFPAVQLRSLQRSPQSPAPPPLPHRGSRSRLPFSSLPVSVAPDRPAGGAWERGTCEQVSSLDEEKTVPRTGSLMSNCWCFIFCKERVRSNSSSPQHDGTSREEADHQVDVSDGIRLVPDKAEATMATASDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALTEGNSILESLREPLAESIVHSVNQDQLDNQATVKRLLTQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTALDSMSQKIAAFTDVGNSLAHVQHLLKDLTTFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRDLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPASTSPTKGSTRNVKKLEDRKTDPLCLEGCVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPRKLVPGKYTVLMDGEKGGSDTLAMRSGDMVEVVEEGTEGLWYVRDLTSSKEGWVPASSLATLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGYPTPFSELQG

Target ORF information:

RefSeq Version	XM_008771381.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008771381.2

ORF Insert Sequence:

ATGTTTAGGT GTGCCCTGGC AATCTACCGT TTGCAGTGGC ATTTAGAGTT ACCTACAGGT 
GGCTACTCAC GAAACGGAAT GTCAAGCAAG CAGTGCGAGT GGAGAAGTGG AGCACCCTCA 
GGTCAGATAA GAACAGAGGC CAGAAAAGCT TATGGAACGT GCATGAAGTC AAAGGGTTCC 
CACGAAGCAG TCTTGGGAGA GCCTGGTCAC CTGAGACTTC GGAGTGGACA CAATTTCCCT 
GCAGTCCAGC TGAGAAGTCT GCAACGGTCG CCTCAGTCCC CAGCGCCACC GCCCCTCCCC 
CACCGTGGCT CCCGCTCCCG CCTTCCCTTC TCCTCCCTCC CTGTCTCAGT GGCCCCAGAC 
CGACCGGCGG GCGGAGCCTG GGAGCGTGGC ACCTGCGAGC AAGTGAGCTC GCTGGACGAG 
GAGAAGACGG TGCCCCGCAC AGGCAGCCTC ATGTCCAACT GCTGGTGTTT CATCTTCTGC 
AAGGAACGAG TTAGAAGCAA TTCTTCATCG CCACAGCACG ATGGGACCTC GAGGGAGGAG 
GCTGATCACC AGGTGGACGT GTCTGATGGC ATCAGGCTGG TTCCAGACAA AGCAGAGGCC 
ACGATGGCCA CAGCCTCAGA TGAAATCATG CACCAGGACA TCGTCCCACT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCAGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATCGGG 
TTCATCTTGG TCATAGACCG AAGACAGGAC AAATGGACTT CTGTGAAGGC GTCAGTCCTG 
CGAATAGCCG CATCATTCCC AGCCAACCTG CAGCTCGTCC TCGTTCTCCG ACCCACGGGC 
TTCTTCCAGA GGACTCTCTC GGACATAGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTACCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ATGGCTACAT TGACAAGTCA 
CAGCTGACCG AGGACCTTGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCGA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCCGA GACTGAGCTG CCCAACGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCTAAA GTGAAGGAGG ATCTGCAGCT GGCGCTGACG 
GAGGGGAACA GCATCCTTGA GAGCCTCAGG GAGCCGCTGG CTGAGAGCAT AGTCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACTGTGAAGA GGCTCCTGAC CCAGCTGAAT 
GAGACCGAGG CTGCCTTTGA CGAGTTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTACAGCTGC GACATTTTGA ACAAGGTTTC CGGGAGGTCA AAACCGCCTT GGATTCCATG 
TCCCAGAAGA TAGCCGCGTT CACAGATGTT GGCAACAGCC TGGCCCATGT GCAGCACCTC 
CTGAAGGACT TGACCACCTT TGAGGAGAAG TCCAGCGTGG CCGTGGACAA GGCCAGAGCC 
CTGTCCCTGG AGGGGCAGCA GCTGATTGAG AACCGGCACT ATGCTGTAGA CTCTATCCAT 
CCGAAGTGTG AGGAACTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGACCTTC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GCATCTTCCT GCTGGCCTCT CAGCCTGTGG ACAAATGCCA ATCTCAGGAT 
GGTGCGGAGG CAGCCCTGCA GGAGATCGAG AAGTTTCTGG AGACCGGTGC AGAAAACAAG 
ATTCAGGAGC TCAATAAGAT TTACAAGGAA TATGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAGAGCACCG AGGAGATGTT CCACCGCAGG 
CAGGCTAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTAACCAA GTCCCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCCCTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAGC CCCGGCAGGG CCGCACCAGC TCCACAGGAG AAGAGGAGGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAACTT CTGGACACGG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCTGCA GAGATGGACA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CACTTCCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATCGACTG CCCAGAGCTG 
GTGGGGAGAT GCTTTCTGGA GAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCCGAGAG CCTGTGGAGA CAGTGCTCTG ACTGTCCCTT CTTCCAGGAG 
TGCCAGAAGA AGCTGGACCA CAAGCTGAGC CTCGACTCCT ACCTGCTGAA GCCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCCGAGG ACCTGCAAGA GGCACTGAGC TCCATCCTGG GCATCCTCAA GGCAGTGAAC 
GACTCCATGC ACCTCATAGC CATCACTGGC TATGATGGAA ACCTTGGGGA CCTGGGGAAG 
CTGCTGATGC AAGGCTCCTT CAGCGTGTGG ACGGACCACA AGAAGGGCCA CACCAAGGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTCT TCCTTCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGGTACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACTGCTGTT GGCATCACAG AGAATGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ACATCATTCA GGCACCAACT 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAACTGCAG 
GCCTGCCGGG AAGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCCTTGCCC 
ACGCCAGCCA GCACCAGTCC CACAAAAGGG AGCACAAGAA ATGTCAAGAA GCTCGAAGAT 
CGGAAGACGG ACCCGCTCTG CCTGGAAGGC TGCGTGAGCT CATCATTACC AAAGCCCCCC 
GAGAAGGGCA AAGACGATGC GGTCCCTAGT TCTACCTCTG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC CTCAAAGGTC AGAAAGCCTC TCCGACCAGT 
CCTGACAAAA AAGCTAAGCG GCATGAAGTA AAGAGCGACC CGACTCCCTT TGGTTTGCGA 
GGCTGGAGCA AAACATCCCA CTCACTGGAG GCCCCTGAGG AGGACGGAGG CTGGTCCAGT 
GCTGAGGAGC TCATCAATTC ATCCGATGCT GAGGAAGACG GAGGAGTGGG CCCTAGGAAG 
CTGGTTCCAG GTAAATACAC AGTGCTGATG GATGGTGAGA AAGGGGGCTC TGACACCCTT 
GCCATGAGGA GTGGAGATAT GGTGGAGGTT GTGGAAGAAG GAACTGAGGG CCTCTGGTAT 
GTTCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT AGCCACCCTC 
CTGGGCAAAT CCAGCTCAGC CCAGTGTCTG AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC 
GTGCTGAGCA ACTCATCCAG CTGCAGCGAG GGCTACCCTA CTCCCTTCTC CGAGCTCCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_017599979.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455468.1 Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa58741
Clone ID Related Accession (Same CDS sequence)	XM_017599979.1
Accession Version	XM_017599979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide exchange factor DBS isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACCTC GTGGGGAGCG CGCTCTGGGC TGCTGTGGCC TCTGCACCTG CCCGGCAGCG 
GCAGGAGATG AAATCATGCA CCAGGACATC GTCCCACTCT GTGCCGCTGA CATCCAGGAG 
CAGCTGAAGA AGCGCTTTGC TTACCTGTCT GGTGGGCGGG GACAGGACGG AAGCCCAGTC 
ATCACCTTCC CAGACTACCC AGCCTTCAGC GAGATCCCGG ACAAGGAGTT CCAGAATGTC 
ATGACCTATC TCACCAGCAT CCCCAGTTTG CAAGATGCTG GCATCGGGTT CATCTTGGTC 
ATAGACCGAA GACAGGACAA ATGGACTTCT GTGAAGGCGT CAGTCCTGCG AATAGCCGCA 
TCATTCCCAG CCAACCTGCA GCTCGTCCTC GTTCTCCGAC CCACGGGCTT CTTCCAGAGG 
ACTCTCTCGG ACATAGCCTT CAAGTTCAAC AGAGATGAGT TTAAGATGAA AGTACCGGTC 
ATGATGCTAA GCTCAGTGCC AGAACTACAT GGCTACATTG ACAAGTCACA GCTGACCGAG 
GACCTTGGGG GGACCCTGGA CTACTGCCAC TCCAGGTGGC TGTGCCACCG CACAGCGATT 
GAAAGCTTCG CCCTGATGGT GAAGCAGACA GCCCAGATGC TGCAGGCCTT TGGGACCGAG 
CTGGCCGAGA CTGAGCTGCC CAACGACGTC CAGTCAACCA GCCTGGTGCT CAGCGCACAC 
ACAGAGAAGA AGGCTAAAGT GAAGGAGGAT CTGCAGCTGG CGCTGACGGA GGGGAACAGC 
ATCCTTGAGA GCCTCAGGGA GCCGCTGGCT GAGAGCATAG TCCACAGTGT GAACCAGGAC 
CAGCTGGACA ATCAGGCCAC TGTGAAGAGG CTCCTGACCC AGCTGAATGA GACCGAGGCT 
GCCTTTGACG AGTTCTGGGC AAAGCATCAG CAGAAGCTGG AACAGTGCCT ACAGCTGCGA 
CATTTTGAAC AAGGTTTCCG GGAGGTCAAA ACCGCCTTGG ATTCCATGTC CCAGAAGATA 
GCCGCGTTCA CAGATGTTGG CAACAGCCTG GCCCATGTGC AGCACCTCCT GAAGGACTTG 
ACCACCTTTG AGGAGAAGTC CAGCGTGGCC GTGGACAAGG CCAGAGCCCT GTCCCTGGAG 
GGGCAGCAGC TGATTGAGAA CCGGCACTAT GCTGTAGACT CTATCCATCC GAAGTGTGAG 
GAACTCCAGC ACCTCTGTGA CCACTTCGCC TCTGAGGTCA CCAGGAGGCG GGACCTTCTC 
AGCAAGTCCT TGGAGCTGCA TAGCCTCCTG GAGACGTCCA TGAAGTGGAG CGACGAGGGC 
ATCTTCCTGC TGGCCTCTCA GCCTGTGGAC AAATGCCAAT CTCAGGATGG TGCGGAGGCA 
GCCCTGCAGG AGATCGAGAA GTTTCTGGAG ACCGGTGCAG AAAACAAGAT TCAGGAGCTC 
AATAAGATTT ACAAGGAATA TGAATGCATC CTCAACCAGG ACCTTCTGGA ACACGTGCAA 
AAAGTCTTTC AGAAGCAAGA GAGCACCGAG GAGATGTTCC ACCGCAGGCA GGCTAGCCTG 
AAGAAACTGG CAGCCAAGCA GACACGGCCT GTGCAGCCCG TGGCCCCCCG ACCAGAGGCG 
CTAACCAAGT CCCCCTCCCC CTCGCCAGGC TCCTGGCGAA GCTCTGAGAA CTCCAGCTCT 
GAGGGCAACG CCCTCCGCAG AGGGCCCTAC AGGAGAGCCA AGAGCGAAAT GAGTGAGCCC 
CGGCAGGGCC GCACCAGCTC CACAGGAGAA GAGGAGGAGA GCCTGGCCAT CCTGCGCAGA 
CACGTGATGA ATGAACTTCT GGACACGGAG CGGGCCTACG TGGAGGAGCT GCTCTGTGTC 
TTAGAGGGCT ATGCTGCAGA GATGGACAAC CCCTTGATGG CACACCTCAT CTCAACAGGC 
CTGCAAAACA AGAAGAACAT TCTGTTTGGA AACATGGAGG AAATCTATCA CTTCCACAAC 
AGAATATTCC TGCGGGAGCT GGAGAGCTGC ATCGACTGCC CAGAGCTGGT GGGGAGATGC 
TTTCTGGAGA GGATGGAGGA GTTCCAGATC TATGAGAAGT ACTGTCAGAA CAAGCCACGC 
TCCGAGAGCC TGTGGAGACA GTGCTCTGAC TGTCCCTTCT TCCAGGAGTG CCAGAAGAAG 
CTGGACCACA AGCTGAGCCT CGACTCCTAC CTGCTGAAGC CTGTGCAGAG GATAACCAAG 
TACCAGCTGC TGCTCAAGGA AATGCTGAAG TACAGCAAGC ACTGTGAAGG GGCCGAGGAC 
CTGCAAGAGG CACTGAGCTC CATCCTGGGC ATCCTCAAGG CAGTGAACGA CTCCATGCAC 
CTCATAGCCA TCACTGGCTA TGATGGAAAC CTTGGGGACC TGGGGAAGCT GCTGATGCAA 
GGCTCCTTCA GCGTGTGGAC GGACCACAAG AAGGGCCACA CCAAGGTGAA GGAGCTAGCC 
AGGTTCAAGC CCATGCAGCG GCACCTCTTC CTTCACGAGA AGGCCGTCCT CTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGGTACGAG AAGGCTCCTT CCTACAGCTA CAAGCAGTCC 
TTGAATATGA CTGCTGTTGG CATCACAGAG AATGTGAAGG GTGACACCAA GAAGTTCGAG 
ATCTGGTACA ACGCAAGGGA GGAGGTTTAC ATCATTCAGG CACCAACTCC CGAGATTAAA 
GCAGCGTGGG TGAATGAGAT TCGGAAGGTG CTGACCAGCC AACTGCAGGC CTGCCGGGAA 
GCCAGCCAGC ACCGAGCCCT GGAGCAGTCC CACAGCCTTC CCTTGCCCAC GCCAGCCAGC 
ACCAGTCCCA CAAAAGGGAG CACAAGAAAT GTCAAGAAGC TCGAAGATCG GAAGACGGAC 
CCGCTCTGCC TGGAAGGCTG CGTGAGCTCA TCATTACCAA AGCCCCCCGA GAAGGGCAAA 
GACGATGCGG TCCCTAGTTC TACCTCTGAA AGCTCTGCGC TTTCCAGAAA GCGCTTCACG 
CTGCAGGGCT TGGCTAACCT CAAAGGTCAG AAAGCCTCTC CGACCAGTCC TGACAAAAAA 
GCTAAGCGGC ATGAAGTAAA GAGCGACCCG ACTCCCTTTG GTTTGCGAGG CTGGAGCAAA 
ACATCCCACT CACTGGAGGC CCCTGAGGAG GACGGAGGCT GGTCCAGTGC TGAGGAGCTC 
ATCAATTCAT CCGATGCTGA GGAAGACGGA GGAGTGGGCC CTAGGAAGCT GGTTCCAGGT 
AAATACACAG TGCTGATGGA TGGTGAGAAA GGGGGCTCTG ACACCCTTGC CATGAGGAGT 
GGAGATATGG TGGAGGTTGT GGAAGAAGGA ACTGAGGGCC TCTGGTATGT TCGGGACCTG 
ACCAGCAGCA AGGAGGGATG GGTGCCAGCC AGCAGCCTAG CCACCCTCCT GGGCAAATCC 
AGCTCAGCCC AGTGTCTGAG CAGCTCAGAC TCCAGCCCAG GGTCAGCCGT GCTGAGCAAC 
TCATCCAGCT GCAGCGAGGG CTACCCTACT CCCTTCTCCG AGCTCCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455468.1
CDS	2545..6078
Translation	MRPRGERALGCCGLCTCPAAAGDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALTEGNSILESLREPLAESIVHSVNQDQLDNQATVKRLLTQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTALDSMSQKIAAFTDVGNSLAHVQHLLKDLTTFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRDLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPASTSPTKGSTRNVKKLEDRKTDPLCLEGCVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPRKLVPGKYTVLMDGEKGGSDTLAMRSGDMVEVVEEGTEGLWYVRDLTSSKEGWVPASSLATLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGYPTPFSELQG

Target ORF information:

RefSeq Version	XM_017599979.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599979.1

ORF Insert Sequence:

ATGAGACCTC GTGGGGAGCG CGCTCTGGGC TGCTGTGGCC TCTGCACCTG CCCGGCAGCG 
GCAGGAGATG AAATCATGCA CCAGGACATC GTCCCACTCT GTGCCGCTGA CATCCAGGAG 
CAGCTGAAGA AGCGCTTTGC TTACCTGTCT GGTGGGCGGG GACAGGACGG AAGCCCAGTC 
ATCACCTTCC CAGACTACCC AGCCTTCAGC GAGATCCCGG ACAAGGAGTT CCAGAATGTC 
ATGACCTATC TCACCAGCAT CCCCAGTTTG CAAGATGCTG GCATCGGGTT CATCTTGGTC 
ATAGACCGAA GACAGGACAA ATGGACTTCT GTGAAGGCGT CAGTCCTGCG AATAGCCGCA 
TCATTCCCAG CCAACCTGCA GCTCGTCCTC GTTCTCCGAC CCACGGGCTT CTTCCAGAGG 
ACTCTCTCGG ACATAGCCTT CAAGTTCAAC AGAGATGAGT TTAAGATGAA AGTACCGGTC 
ATGATGCTAA GCTCAGTGCC AGAACTACAT GGCTACATTG ACAAGTCACA GCTGACCGAG 
GACCTTGGGG GGACCCTGGA CTACTGCCAC TCCAGGTGGC TGTGCCACCG CACAGCGATT 
GAAAGCTTCG CCCTGATGGT GAAGCAGACA GCCCAGATGC TGCAGGCCTT TGGGACCGAG 
CTGGCCGAGA CTGAGCTGCC CAACGACGTC CAGTCAACCA GCCTGGTGCT CAGCGCACAC 
ACAGAGAAGA AGGCTAAAGT GAAGGAGGAT CTGCAGCTGG CGCTGACGGA GGGGAACAGC 
ATCCTTGAGA GCCTCAGGGA GCCGCTGGCT GAGAGCATAG TCCACAGTGT GAACCAGGAC 
CAGCTGGACA ATCAGGCCAC TGTGAAGAGG CTCCTGACCC AGCTGAATGA GACCGAGGCT 
GCCTTTGACG AGTTCTGGGC AAAGCATCAG CAGAAGCTGG AACAGTGCCT ACAGCTGCGA 
CATTTTGAAC AAGGTTTCCG GGAGGTCAAA ACCGCCTTGG ATTCCATGTC CCAGAAGATA 
GCCGCGTTCA CAGATGTTGG CAACAGCCTG GCCCATGTGC AGCACCTCCT GAAGGACTTG 
ACCACCTTTG AGGAGAAGTC CAGCGTGGCC GTGGACAAGG CCAGAGCCCT GTCCCTGGAG 
GGGCAGCAGC TGATTGAGAA CCGGCACTAT GCTGTAGACT CTATCCATCC GAAGTGTGAG 
GAACTCCAGC ACCTCTGTGA CCACTTCGCC TCTGAGGTCA CCAGGAGGCG GGACCTTCTC 
AGCAAGTCCT TGGAGCTGCA TAGCCTCCTG GAGACGTCCA TGAAGTGGAG CGACGAGGGC 
ATCTTCCTGC TGGCCTCTCA GCCTGTGGAC AAATGCCAAT CTCAGGATGG TGCGGAGGCA 
GCCCTGCAGG AGATCGAGAA GTTTCTGGAG ACCGGTGCAG AAAACAAGAT TCAGGAGCTC 
AATAAGATTT ACAAGGAATA TGAATGCATC CTCAACCAGG ACCTTCTGGA ACACGTGCAA 
AAAGTCTTTC AGAAGCAAGA GAGCACCGAG GAGATGTTCC ACCGCAGGCA GGCTAGCCTG 
AAGAAACTGG CAGCCAAGCA GACACGGCCT GTGCAGCCCG TGGCCCCCCG ACCAGAGGCG 
CTAACCAAGT CCCCCTCCCC CTCGCCAGGC TCCTGGCGAA GCTCTGAGAA CTCCAGCTCT 
GAGGGCAACG CCCTCCGCAG AGGGCCCTAC AGGAGAGCCA AGAGCGAAAT GAGTGAGCCC 
CGGCAGGGCC GCACCAGCTC CACAGGAGAA GAGGAGGAGA GCCTGGCCAT CCTGCGCAGA 
CACGTGATGA ATGAACTTCT GGACACGGAG CGGGCCTACG TGGAGGAGCT GCTCTGTGTC 
TTAGAGGGCT ATGCTGCAGA GATGGACAAC CCCTTGATGG CACACCTCAT CTCAACAGGC 
CTGCAAAACA AGAAGAACAT TCTGTTTGGA AACATGGAGG AAATCTATCA CTTCCACAAC 
AGAATATTCC TGCGGGAGCT GGAGAGCTGC ATCGACTGCC CAGAGCTGGT GGGGAGATGC 
TTTCTGGAGA GGATGGAGGA GTTCCAGATC TATGAGAAGT ACTGTCAGAA CAAGCCACGC 
TCCGAGAGCC TGTGGAGACA GTGCTCTGAC TGTCCCTTCT TCCAGGAGTG CCAGAAGAAG 
CTGGACCACA AGCTGAGCCT CGACTCCTAC CTGCTGAAGC CTGTGCAGAG GATAACCAAG 
TACCAGCTGC TGCTCAAGGA AATGCTGAAG TACAGCAAGC ACTGTGAAGG GGCCGAGGAC 
CTGCAAGAGG CACTGAGCTC CATCCTGGGC ATCCTCAAGG CAGTGAACGA CTCCATGCAC 
CTCATAGCCA TCACTGGCTA TGATGGAAAC CTTGGGGACC TGGGGAAGCT GCTGATGCAA 
GGCTCCTTCA GCGTGTGGAC GGACCACAAG AAGGGCCACA CCAAGGTGAA GGAGCTAGCC 
AGGTTCAAGC CCATGCAGCG GCACCTCTTC CTTCACGAGA AGGCCGTCCT CTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGGTACGAG AAGGCTCCTT CCTACAGCTA CAAGCAGTCC 
TTGAATATGA CTGCTGTTGG CATCACAGAG AATGTGAAGG GTGACACCAA GAAGTTCGAG 
ATCTGGTACA ACGCAAGGGA GGAGGTTTAC ATCATTCAGG CACCAACTCC CGAGATTAAA 
GCAGCGTGGG TGAATGAGAT TCGGAAGGTG CTGACCAGCC AACTGCAGGC CTGCCGGGAA 
GCCAGCCAGC ACCGAGCCCT GGAGCAGTCC CACAGCCTTC CCTTGCCCAC GCCAGCCAGC 
ACCAGTCCCA CAAAAGGGAG CACAAGAAAT GTCAAGAAGC TCGAAGATCG GAAGACGGAC 
CCGCTCTGCC TGGAAGGCTG CGTGAGCTCA TCATTACCAA AGCCCCCCGA GAAGGGCAAA 
GACGATGCGG TCCCTAGTTC TACCTCTGAA AGCTCTGCGC TTTCCAGAAA GCGCTTCACG 
CTGCAGGGCT TGGCTAACCT CAAAGGTCAG AAAGCCTCTC CGACCAGTCC TGACAAAAAA 
GCTAAGCGGC ATGAAGTAAA GAGCGACCCG ACTCCCTTTG GTTTGCGAGG CTGGAGCAAA 
ACATCCCACT CACTGGAGGC CCCTGAGGAG GACGGAGGCT GGTCCAGTGC TGAGGAGCTC 
ATCAATTCAT CCGATGCTGA GGAAGACGGA GGAGTGGGCC CTAGGAAGCT GGTTCCAGGT 
AAATACACAG TGCTGATGGA TGGTGAGAAA GGGGGCTCTG ACACCCTTGC CATGAGGAGT 
GGAGATATGG TGGAGGTTGT GGAAGAAGGA ACTGAGGGCC TCTGGTATGT TCGGGACCTG 
ACCAGCAGCA AGGAGGGATG GGTGCCAGCC AGCAGCCTAG CCACCCTCCT GGGCAAATCC 
AGCTCAGCCC AGTGTCTGAG CAGCTCAGAC TCCAGCCCAG GGTCAGCCGT GCTGAGCAAC 
TCATCCAGCT GCAGCGAGGG CTACCCTACT CCCTTCTCCG AGCTCCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( NM_053951.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446403.2 Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), mRNA.

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CloneID	ORa11158
Clone ID Related Accession (Same CDS sequence)	NM_053951.2
Accession Version	NM_053951.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide exchange factor DBS
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473970.2. On Jan 11, 2008 this sequence version replaced NM_053951.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCACT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCAGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATCGGG 
TTCATCTTGG TCATAGACCG AAGACAGGAC AAATGGACTT CTGTGAAGGC GTCAGTCCTG 
CGAATAGCCG CATCATTCCC AGCCAACCTG CAGCTCGTCC TCGTTCTCCG ACCCACGGGC 
TTCTTCCAGA GGACTCTCTC GGACATAGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTACCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ATGGCTACAT TGACAAGTCA 
CAGCTGACCG AGGACCTTGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCGA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCCGA GACTGAGCTG CCCAACGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCTAAA GTGAAGGAGG ATCTGCAGCT GGCGCTGACG 
GAGGGGAACA GCATCCTTGA GAGCCTCAGG GAGCCGCTGG CTGAGAGCAT AGTCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACTGTGAAGA GGCTCCTGAC CCAGCTGAAT 
GAGACCGAGG CTGCCTTTGA CGAGTTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTACAGCTGC GACATTTTGA ACAAGGTTTC CGGGAGGTCA AAACCGCCTT GGATTCCATG 
TCCCAGAAGA TAGCCGCGTT CACAGATGTT GGCAACAGCC TGGCCCATGT GCAGCACCTC 
CTGAAGGACT TGACCACCTT TGAGGAGAAG TCCAGCGTGG CCGTGGACAA GGCCAGAGCC 
CTGTCCCTGG AGGGGCAGCA GCTGATTGAG AACCGGCACT ATGCTGTAGA CTCTATCCAT 
CCGAAGTGTG AGGAACTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGACCTTC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GCATCTTCCT GCTGGCCTCT CAGCCTGTGG ACAAATGCCA ATCTCAGGAT 
GGTGCGGAGG CAGCCCTGCA GGAGATCGAG AAGTTTCTGG AGACCGGTGC AGAAAACAAG 
ATTCAGGAGC TCAATAAGAT TTACAAGGAA TATGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAGAGCACCG AGGAGATGTT CCACCGCAGG 
CAGGCTAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTAACCAA GTCCCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCCCTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAGC CCCGGCAGGG CCGCACCAGC TCCACAGGAG AAGAGGAGGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAACTT CTGGACACGG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCTGCA GAGATGGACA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CACTTCCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATCGACTG CCCAGAGCTG 
GTGGGGAGAT GCTTTCTGGA GAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCCGAGAG CCTGTGGAGA CAGTGCTCTG ACTGTCCCTT CTTCCAGGAG 
TGCCAGAAGA AGCTGGACCA CAAGCTGAGC CTCGACTCCT ACCTGCTGAA GCCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCCGAGG ACCTGCAAGA GGCACTGAGC TCCATCCTGG GCATCCTCAA GGCAGTGAAC 
GACTCCATGC ACCTCATAGC CATCACTGGC TATGATGGAA ACCTTGGGGA CCTGGGGAAG 
CTGCTGATGC AAGGCTCCTT CAGCGTGTGG ACGGACCACA AGAAGGGCCA CACCAAGGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTCT TCCTTCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGGTACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACTGCTGTT GGCATCACAG AGAATGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ACATCATTCA GGCACCAACT 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAACTGCAG 
GCCTGCCGGG AAGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCCTTGCCC 
ACGCCAGCCA GCACCAGTCC CACAAAAGGG AGCACAAGAA ATGTCAAGAA GCTCGAAGAT 
CGGAAGACGG ACCCGCTCTG CCTGGAAGGC TGCGTGAGCT CATCATTACC AAAGCCCCCC 
GAGAAGGGCA AAGACGATGC GGTCCCTAGT TCTACCTCTG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC CTCAAAGCCT CTCCGACCAG TCCTGACAAA 
AAAGCTAAGC GGCATGAAGT AAAGAGCGAC CCGACTCCCT TTGGTTTGCG AGGCTGGAGC 
AAAACATCCC ACTCACTGGA GGCCCCTGAG GAGGACGGAG GCTGGTCCAG TGCTGAGGAG 
CTCATCAATT CATCCGATGC TGAGGAAGAC GGAGGAGTGG GCCCTAGGAA GCTGGTTCCA 
GGTAAATACA CAGTGCTGAT GGATGGTGAG AAAGGGGGCT CTGACACCCT TGCCATGAGG 
AGTGGAGATA TGGTGGAGGT TGTGGAAGAA GGAACTGAGG GCCTCTGGTA TGTTCGGGAC 
CTGACCAGCA GCAAGGAGGG ATGGGTGCCA GCCAGCAGCC TAGCCACCCT CCTGGGCAAA 
TCCAGCTCAG CCCAGTGTCT GAGCAGCTCA GGTAAGACCC ACTGTGCGCG CCAGCTGTGC 
CCTGAGCCTG CCAAGATCCT CAGCCCAGAG CCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446403.2
CDS	19..3537
Translation	MRFWLRNEEMALEEMVQRLNAVSKNTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALTEGNSILESLREPLAESIVHSVNQDQLDNQATVKRLLTQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTALDSMSQKIAAFTDVGNSLAHVQHLLKDLTTFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRDLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPASTSPTKGSTRNVKKLEDRKTDPLCLEGCVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPRKLVPGKYTVLMDGEKGGSDTLAMRSGDMVEVVEEGTEGLWYVRDLTSSKEGWVPASSLATLLGKSSSAQCLSSSGKTHCARQLCPEPAKILSPEPV

Target ORF information:

RefSeq Version	NM_053951.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053951.2

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCACT CTGTGCCGCT 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGACAGGAC 
GGAAGCCCAG TCATCACCTT CCCAGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATCGGG 
TTCATCTTGG TCATAGACCG AAGACAGGAC AAATGGACTT CTGTGAAGGC GTCAGTCCTG 
CGAATAGCCG CATCATTCCC AGCCAACCTG CAGCTCGTCC TCGTTCTCCG ACCCACGGGC 
TTCTTCCAGA GGACTCTCTC GGACATAGCC TTCAAGTTCA ACAGAGATGA GTTTAAGATG 
AAAGTACCGG TCATGATGCT AAGCTCAGTG CCAGAACTAC ATGGCTACAT TGACAAGTCA 
CAGCTGACCG AGGACCTTGG GGGGACCCTG GACTACTGCC ACTCCAGGTG GCTGTGCCAC 
CGCACAGCGA TTGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCCCAGAT GCTGCAGGCC 
TTTGGGACCG AGCTGGCCGA GACTGAGCTG CCCAACGACG TCCAGTCAAC CAGCCTGGTG 
CTCAGCGCAC ACACAGAGAA GAAGGCTAAA GTGAAGGAGG ATCTGCAGCT GGCGCTGACG 
GAGGGGAACA GCATCCTTGA GAGCCTCAGG GAGCCGCTGG CTGAGAGCAT AGTCCACAGT 
GTGAACCAGG ACCAGCTGGA CAATCAGGCC ACTGTGAAGA GGCTCCTGAC CCAGCTGAAT 
GAGACCGAGG CTGCCTTTGA CGAGTTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTACAGCTGC GACATTTTGA ACAAGGTTTC CGGGAGGTCA AAACCGCCTT GGATTCCATG 
TCCCAGAAGA TAGCCGCGTT CACAGATGTT GGCAACAGCC TGGCCCATGT GCAGCACCTC 
CTGAAGGACT TGACCACCTT TGAGGAGAAG TCCAGCGTGG CCGTGGACAA GGCCAGAGCC 
CTGTCCCTGG AGGGGCAGCA GCTGATTGAG AACCGGCACT ATGCTGTAGA CTCTATCCAT 
CCGAAGTGTG AGGAACTCCA GCACCTCTGT GACCACTTCG CCTCTGAGGT CACCAGGAGG 
CGGGACCTTC TCAGCAAGTC CTTGGAGCTG CATAGCCTCC TGGAGACGTC CATGAAGTGG 
AGCGACGAGG GCATCTTCCT GCTGGCCTCT CAGCCTGTGG ACAAATGCCA ATCTCAGGAT 
GGTGCGGAGG CAGCCCTGCA GGAGATCGAG AAGTTTCTGG AGACCGGTGC AGAAAACAAG 
ATTCAGGAGC TCAATAAGAT TTACAAGGAA TATGAATGCA TCCTCAACCA GGACCTTCTG 
GAACACGTGC AAAAAGTCTT TCAGAAGCAA GAGAGCACCG AGGAGATGTT CCACCGCAGG 
CAGGCTAGCC TGAAGAAACT GGCAGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCC 
CGACCAGAGG CGCTAACCAA GTCCCCCTCC CCCTCGCCAG GCTCCTGGCG AAGCTCTGAG 
AACTCCAGCT CTGAGGGCAA CGCCCTCCGC AGAGGGCCCT ACAGGAGAGC CAAGAGCGAA 
ATGAGTGAGC CCCGGCAGGG CCGCACCAGC TCCACAGGAG AAGAGGAGGA GAGCCTGGCC 
ATCCTGCGCA GACACGTGAT GAATGAACTT CTGGACACGG AGCGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TCTTAGAGGG CTATGCTGCA GAGATGGACA ACCCCTTGAT GGCACACCTC 
ATCTCAACAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAAATCTAT 
CACTTCCACA ACAGAATATT CCTGCGGGAG CTGGAGAGCT GCATCGACTG CCCAGAGCTG 
GTGGGGAGAT GCTTTCTGGA GAGGATGGAG GAGTTCCAGA TCTATGAGAA GTACTGTCAG 
AACAAGCCAC GCTCCGAGAG CCTGTGGAGA CAGTGCTCTG ACTGTCCCTT CTTCCAGGAG 
TGCCAGAAGA AGCTGGACCA CAAGCTGAGC CTCGACTCCT ACCTGCTGAA GCCTGTGCAG 
AGGATAACCA AGTACCAGCT GCTGCTCAAG GAAATGCTGA AGTACAGCAA GCACTGTGAA 
GGGGCCGAGG ACCTGCAAGA GGCACTGAGC TCCATCCTGG GCATCCTCAA GGCAGTGAAC 
GACTCCATGC ACCTCATAGC CATCACTGGC TATGATGGAA ACCTTGGGGA CCTGGGGAAG 
CTGCTGATGC AAGGCTCCTT CAGCGTGTGG ACGGACCACA AGAAGGGCCA CACCAAGGTG 
AAGGAGCTAG CCAGGTTCAA GCCCATGCAG CGGCACCTCT TCCTTCACGA GAAGGCCGTC 
CTCTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGGTACG AGAAGGCTCC TTCCTACAGC 
TACAAGCAGT CCTTGAATAT GACTGCTGTT GGCATCACAG AGAATGTGAA GGGTGACACC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGG GAGGAGGTTT ACATCATTCA GGCACCAACT 
CCCGAGATTA AAGCAGCGTG GGTGAATGAG ATTCGGAAGG TGCTGACCAG CCAACTGCAG 
GCCTGCCGGG AAGCCAGCCA GCACCGAGCC CTGGAGCAGT CCCACAGCCT TCCCTTGCCC 
ACGCCAGCCA GCACCAGTCC CACAAAAGGG AGCACAAGAA ATGTCAAGAA GCTCGAAGAT 
CGGAAGACGG ACCCGCTCTG CCTGGAAGGC TGCGTGAGCT CATCATTACC AAAGCCCCCC 
GAGAAGGGCA AAGACGATGC GGTCCCTAGT TCTACCTCTG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC CTCAAAGCCT CTCCGACCAG TCCTGACAAA 
AAAGCTAAGC GGCATGAAGT AAAGAGCGAC CCGACTCCCT TTGGTTTGCG AGGCTGGAGC 
AAAACATCCC ACTCACTGGA GGCCCCTGAG GAGGACGGAG GCTGGTCCAG TGCTGAGGAG 
CTCATCAATT CATCCGATGC TGAGGAAGAC GGAGGAGTGG GCCCTAGGAA GCTGGTTCCA 
GGTAAATACA CAGTGCTGAT GGATGGTGAG AAAGGGGGCT CTGACACCCT TGCCATGAGG 
AGTGGAGATA TGGTGGAGGT TGTGGAAGAA GGAACTGAGG GCCTCTGGTA TGTTCGGGAC 
CTGACCAGCA GCAAGGAGGG ATGGGTGCCA GCCAGCAGCC TAGCCACCCT CCTGGGCAAA 
TCCAGCTCAG CCCAGTGTCT GAGCAGCTCA GGTAAGACCC ACTGTGCGCG CCAGCTGTGC 
CCTGAGCCTG CCAAGATCCT CAGCCCAGAG CCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006253416.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253478.1 Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa37550
Clone ID Related Accession (Same CDS sequence)	XM_006253416.3
Accession Version	XM_006253416.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006253416.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACTA GCCTCCCAGG TGCTCAGGTG GTGCACACGC ATGACTCCAG GTGCGGGGTG 
GTGACACGCC CGGCAGGCTC AGTGCTTCCG CTTCCGCCTA GCGCCCCCCG CTCCCCCATC 
AACGCAAAGC GCGCCCCCCT CCCTGTGGCG CGGAGACCAA TCCTGGGAAG CCAAGGAAAG 
CGCCACAACC CCCTCCCCGC TCCAGCCGCG CCCCCTCCCC ACGCTCAGCC CCACCCGACA 
GCACCTCTGG TGCAGCGGAG GACGATCTTC TGGGGATCGG TTTTGTTGAG TCGGAGAATG 
AGGTTTTGGC TGAGAAATGA AGAGATGGCC CTAGAAGAAA TGGTGCAGAG ATTAAACGCG 
GTTTCCAAGA ACACTGATGA AATCATGCAC CAGGACATCG TCCCACTCTG TGCCGCTGAC 
ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT TACCTGTCTG GTGGGCGGGG ACAGGACGGA 
AGCCCAGTCA TCACCTTCCC AGACTACCCA GCCTTCAGCG AGATCCCGGA CAAGGAGTTC 
CAGAATGTCA TGACCTATCT CACCAGCATC CCCAGTTTGC AAGATGCTGG CATCGGGTTC 
ATCTTGGTCA TAGACCGAAG ACAGGACAAA TGGACTTCTG TGAAGGCGTC AGTCCTGCGA 
ATAGCCGCAT CATTCCCAGC CAACCTGCAG CTCGTCCTCG TTCTCCGACC CACGGGCTTC 
TTCCAGAGGA CTCTCTCGGA CATAGCCTTC AAGTTCAACA GAGATGAGTT TAAGATGAAA 
GTACCGGTCA TGATGCTAAG CTCAGTGCCA GAACTACATG GCTACATTGA CAAGTCACAG 
CTGACCGAGG ACCTTGGGGG GACCCTGGAC TACTGCCACT CCAGGTGGCT GTGCCACCGC 
ACAGCGATTG AAAGCTTCGC CCTGATGGTG AAGCAGACAG CCCAGATGCT GCAGGCCTTT 
GGGACCGAGC TGGCCGAGAC TGAGCTGCCC AACGACGTCC AGTCAACCAG CCTGGTGCTC 
AGCGCACACA CAGAGAAGAA GGCTAAAGTG AAGGAGGATC TGCAGCTGGC GCTGACGGAG 
GGGAACAGCA TCCTTGAGAG CCTCAGGGAG CCGCTGGCTG AGAGCATAGT CCACAGTGTG 
AACCAGGACC AGCTGGACAA TCAGGCCACT GTGAAGAGGC TCCTGACCCA GCTGAATGAG 
ACCGAGGCTG CCTTTGACGA GTTCTGGGCA AAGCATCAGC AGAAGCTGGA ACAGTGCCTA 
CAGCTGCGAC ATTTTGAACA AGGTTTCCGG GAGGTCAAAA CCGCCTTGGA TTCCATGTCC 
CAGAAGATAG CCGCGTTCAC AGATGTTGGC AACAGCCTGG CCCATGTGCA GCACCTCCTG 
AAGGACTTGA CCACCTTTGA GGAGAAGTCC AGCGTGGCCG TGGACAAGGC CAGAGCCCTG 
TCCCTGGAGG GGCAGCAGCT GATTGAGAAC CGGCACTATG CTGTAGACTC TATCCATCCG 
AAGTGTGAGG AACTCCAGCA CCTCTGTGAC CACTTCGCCT CTGAGGTCAC CAGGAGGCGG 
GACCTTCTCA GCAAGTCCTT GGAGCTGCAT AGCCTCCTGG AGACGTCCAT GAAGTGGAGC 
GACGAGGGCA TCTTCCTGCT GGCCTCTCAG CCTGTGGACA AATGCCAATC TCAGGATGGT 
GCGGAGGCAG CCCTGCAGGA GATCGAGAAG TTTCTGGAGA CCGGTGCAGA AAACAAGATT 
CAGGAGCTCA ATAAGATTTA CAAGGAATAT GAATGCATCC TCAACCAGGA CCTTCTGGAA 
CACGTGCAAA AAGTCTTTCA GAAGCAAGAG AGCACCGAGG AGATGTTCCA CCGCAGGCAG 
GCTAGCCTGA AGAAACTGGC AGCCAAGCAG ACACGGCCTG TGCAGCCCGT GGCCCCCCGA 
CCAGAGGCGC TAACCAAGTC CCCCTCCCCC TCGCCAGGCT CCTGGCGAAG CTCTGAGAAC 
TCCAGCTCTG AGGGCAACGC CCTCCGCAGA GGGCCCTACA GGAGAGCCAA GAGCGAAATG 
AGTGAGCCCC GGCAGGGCCG CACCAGCTCC ACAGGAGAAG AGGAGGAGAG CCTGGCCATC 
CTGCGCAGAC ACGTGATGAA TGAACTTCTG GACACGGAGC GGGCCTACGT GGAGGAGCTG 
CTCTGTGTCT TAGAGGGCTA TGCTGCAGAG ATGGACAACC CCTTGATGGC ACACCTCATC 
TCAACAGGCC TGCAAAACAA GAAGAACATT CTGTTTGGAA ACATGGAGGA AATCTATCAC 
TTCCACAACA GAATATTCCT GCGGGAGCTG GAGAGCTGCA TCGACTGCCC AGAGCTGGTG 
GGGAGATGCT TTCTGGAGAG GATGGAGGAG TTCCAGATCT ATGAGAAGTA CTGTCAGAAC 
AAGCCACGCT CCGAGAGCCT GTGGAGACAG TGCTCTGACT GTCCCTTCTT CCAGGAGTGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTC GACTCCTACC TGCTGAAGCC TGTGCAGAGG 
ATAACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAGT ACAGCAAGCA CTGTGAAGGG 
GCCGAGGACC TGCAAGAGGC ACTGAGCTCC ATCCTGGGCA TCCTCAAGGC AGTGAACGAC 
TCCATGCACC TCATAGCCAT CACTGGCTAT GATGGAAACC TTGGGGACCT GGGGAAGCTG 
CTGATGCAAG GCTCCTTCAG CGTGTGGACG GACCACAAGA AGGGCCACAC CAAGGTGAAG 
GAGCTAGCCA GGTTCAAGCC CATGCAGCGG CACCTCTTCC TTCACGAGAA GGCCGTCCTC 
TTCTGCAAGA AGAGGGAGGA GAATGGGGAG GGGTACGAGA AGGCTCCTTC CTACAGCTAC 
AAGCAGTCCT TGAATATGAC TGCTGTTGGC ATCACAGAGA ATGTGAAGGG TGACACCAAG 
AAGTTCGAGA TCTGGTACAA CGCAAGGGAG GAGGTTTACA TCATTCAGGC ACCAACTCCC 
GAGATTAAAG CAGCGTGGGT GAATGAGATT CGGAAGGTGC TGACCAGCCA ACTGCAGGCC 
TGCCGGGAAG CCAGCCAGCA CCGAGCCCTG GAGCAGTCCC ACAGCCTTCC CTTGCCCACG 
CCAGCCAGCA CCAGTCCCAC AAAAGGGAGC ACAAGAAATG TCAAGAAGCT CGAAGATCGG 
AAGACGGACC CGCTCTGCCT GGAAGGCTGC GTGAGCTCAT CATTACCAAA GCCCCCCGAG 
AAGGGCAAAG ACGATGCGGT CCCTAGTTCT ACCTCTGAAA GCTCTGCGCT TTCCAGAAAG 
CGCTTCACGC TGCAGGGCTT GGCTAACCTC AAAGGTCAGA AAGCCTCTCC GACCAGTCCT 
GACAAAAAAG CTAAGCGGCA TGAAGTAAAG AGCGACCCGA CTCCCTTTGG TTTGCGAGGC 
TGGAGCAAAA CATCCCACTC ACTGGAGGCC CCTGAGGAGG ACGGAGGCTG GTCCAGTGCT 
GAGGAGCTCA TCAATTCATC CGATGCTGAG GAAGACGGAG GAGTGGGCCC TAGGAAGCTG 
GTTCCAGGTA AATACACAGT GCTGATGGAT GGTGAGAAAG GGGGCTCTGA CACCCTTGCC 
ATGAGGAGTG GAGATATGGT GGAGGTTGTG GAAGAAGGAA CTGAGGGCCT CTGGTATGTT 
CGGGACCTGA CCAGCAGCAA GGAGGGATGG GTGCCAGCCA GCAGCCTAGC CACCCTCCTG 
GGCAAATCCA GCTCAGCCCA GTGTCTGAGC AGCTCAGACT CCAGCCCAGG GTCAGCCGTG 
CTGAGCAACT CATCCAGCTG CAGCGAGGGC TACCCTACTC CCTTCTCCGA GCTCCAGGGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253478.1
CDS	167..4009
Translation	MQTSLPGAQVVHTHDSRCGVVTRPAGSVLPLPPSAPRSPINAKRAPLPVARRPILGSQGKRHNPLPAPAAPPPHAQPHPTAPLVQRRTIFWGSVLLSRRMRFWLRNEEMALEEMVQRLNAVSKNTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALTEGNSILESLREPLAESIVHSVNQDQLDNQATVKRLLTQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTALDSMSQKIAAFTDVGNSLAHVQHLLKDLTTFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRDLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPASTSPTKGSTRNVKKLEDRKTDPLCLEGCVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPRKLVPGKYTVLMDGEKGGSDTLAMRSGDMVEVVEEGTEGLWYVRDLTSSKEGWVPASSLATLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGYPTPFSELQG

Target ORF information:

RefSeq Version	XM_006253416.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253416.3

ORF Insert Sequence:

ATGCAAACTA GCCTCCCAGG TGCTCAGGTG GTGCACACGC ATGACTCCAG GTGCGGGGTG 
GTGACACGCC CGGCAGGCTC AGTGCTTCCG CTTCCGCCTA GCGCCCCCCG CTCCCCCATC 
AACGCAAAGC GCGCCCCCCT CCCTGTGGCG CGGAGACCAA TCCTGGGAAG CCAAGGAAAG 
CGCCACAACC CCCTCCCCGC TCCAGCCGCG CCCCCTCCCC ACGCTCAGCC CCACCCGACA 
GCACCTCTGG TGCAGCGGAG GACGATCTTC TGGGGATCGG TTTTGTTGAG TCGGAGAATG 
AGGTTTTGGC TGAGAAATGA AGAGATGGCC CTAGAAGAAA TGGTGCAGAG ATTAAACGCG 
GTTTCCAAGA ACACTGATGA AATCATGCAC CAGGACATCG TCCCACTCTG TGCCGCTGAC 
ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT TACCTGTCTG GTGGGCGGGG ACAGGACGGA 
AGCCCAGTCA TCACCTTCCC AGACTACCCA GCCTTCAGCG AGATCCCGGA CAAGGAGTTC 
CAGAATGTCA TGACCTATCT CACCAGCATC CCCAGTTTGC AAGATGCTGG CATCGGGTTC 
ATCTTGGTCA TAGACCGAAG ACAGGACAAA TGGACTTCTG TGAAGGCGTC AGTCCTGCGA 
ATAGCCGCAT CATTCCCAGC CAACCTGCAG CTCGTCCTCG TTCTCCGACC CACGGGCTTC 
TTCCAGAGGA CTCTCTCGGA CATAGCCTTC AAGTTCAACA GAGATGAGTT TAAGATGAAA 
GTACCGGTCA TGATGCTAAG CTCAGTGCCA GAACTACATG GCTACATTGA CAAGTCACAG 
CTGACCGAGG ACCTTGGGGG GACCCTGGAC TACTGCCACT CCAGGTGGCT GTGCCACCGC 
ACAGCGATTG AAAGCTTCGC CCTGATGGTG AAGCAGACAG CCCAGATGCT GCAGGCCTTT 
GGGACCGAGC TGGCCGAGAC TGAGCTGCCC AACGACGTCC AGTCAACCAG CCTGGTGCTC 
AGCGCACACA CAGAGAAGAA GGCTAAAGTG AAGGAGGATC TGCAGCTGGC GCTGACGGAG 
GGGAACAGCA TCCTTGAGAG CCTCAGGGAG CCGCTGGCTG AGAGCATAGT CCACAGTGTG 
AACCAGGACC AGCTGGACAA TCAGGCCACT GTGAAGAGGC TCCTGACCCA GCTGAATGAG 
ACCGAGGCTG CCTTTGACGA GTTCTGGGCA AAGCATCAGC AGAAGCTGGA ACAGTGCCTA 
CAGCTGCGAC ATTTTGAACA AGGTTTCCGG GAGGTCAAAA CCGCCTTGGA TTCCATGTCC 
CAGAAGATAG CCGCGTTCAC AGATGTTGGC AACAGCCTGG CCCATGTGCA GCACCTCCTG 
AAGGACTTGA CCACCTTTGA GGAGAAGTCC AGCGTGGCCG TGGACAAGGC CAGAGCCCTG 
TCCCTGGAGG GGCAGCAGCT GATTGAGAAC CGGCACTATG CTGTAGACTC TATCCATCCG 
AAGTGTGAGG AACTCCAGCA CCTCTGTGAC CACTTCGCCT CTGAGGTCAC CAGGAGGCGG 
GACCTTCTCA GCAAGTCCTT GGAGCTGCAT AGCCTCCTGG AGACGTCCAT GAAGTGGAGC 
GACGAGGGCA TCTTCCTGCT GGCCTCTCAG CCTGTGGACA AATGCCAATC TCAGGATGGT 
GCGGAGGCAG CCCTGCAGGA GATCGAGAAG TTTCTGGAGA CCGGTGCAGA AAACAAGATT 
CAGGAGCTCA ATAAGATTTA CAAGGAATAT GAATGCATCC TCAACCAGGA CCTTCTGGAA 
CACGTGCAAA AAGTCTTTCA GAAGCAAGAG AGCACCGAGG AGATGTTCCA CCGCAGGCAG 
GCTAGCCTGA AGAAACTGGC AGCCAAGCAG ACACGGCCTG TGCAGCCCGT GGCCCCCCGA 
CCAGAGGCGC TAACCAAGTC CCCCTCCCCC TCGCCAGGCT CCTGGCGAAG CTCTGAGAAC 
TCCAGCTCTG AGGGCAACGC CCTCCGCAGA GGGCCCTACA GGAGAGCCAA GAGCGAAATG 
AGTGAGCCCC GGCAGGGCCG CACCAGCTCC ACAGGAGAAG AGGAGGAGAG CCTGGCCATC 
CTGCGCAGAC ACGTGATGAA TGAACTTCTG GACACGGAGC GGGCCTACGT GGAGGAGCTG 
CTCTGTGTCT TAGAGGGCTA TGCTGCAGAG ATGGACAACC CCTTGATGGC ACACCTCATC 
TCAACAGGCC TGCAAAACAA GAAGAACATT CTGTTTGGAA ACATGGAGGA AATCTATCAC 
TTCCACAACA GAATATTCCT GCGGGAGCTG GAGAGCTGCA TCGACTGCCC AGAGCTGGTG 
GGGAGATGCT TTCTGGAGAG GATGGAGGAG TTCCAGATCT ATGAGAAGTA CTGTCAGAAC 
AAGCCACGCT CCGAGAGCCT GTGGAGACAG TGCTCTGACT GTCCCTTCTT CCAGGAGTGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTC GACTCCTACC TGCTGAAGCC TGTGCAGAGG 
ATAACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAGT ACAGCAAGCA CTGTGAAGGG 
GCCGAGGACC TGCAAGAGGC ACTGAGCTCC ATCCTGGGCA TCCTCAAGGC AGTGAACGAC 
TCCATGCACC TCATAGCCAT CACTGGCTAT GATGGAAACC TTGGGGACCT GGGGAAGCTG 
CTGATGCAAG GCTCCTTCAG CGTGTGGACG GACCACAAGA AGGGCCACAC CAAGGTGAAG 
GAGCTAGCCA GGTTCAAGCC CATGCAGCGG CACCTCTTCC TTCACGAGAA GGCCGTCCTC 
TTCTGCAAGA AGAGGGAGGA GAATGGGGAG GGGTACGAGA AGGCTCCTTC CTACAGCTAC 
AAGCAGTCCT TGAATATGAC TGCTGTTGGC ATCACAGAGA ATGTGAAGGG TGACACCAAG 
AAGTTCGAGA TCTGGTACAA CGCAAGGGAG GAGGTTTACA TCATTCAGGC ACCAACTCCC 
GAGATTAAAG CAGCGTGGGT GAATGAGATT CGGAAGGTGC TGACCAGCCA ACTGCAGGCC 
TGCCGGGAAG CCAGCCAGCA CCGAGCCCTG GAGCAGTCCC ACAGCCTTCC CTTGCCCACG 
CCAGCCAGCA CCAGTCCCAC AAAAGGGAGC ACAAGAAATG TCAAGAAGCT CGAAGATCGG 
AAGACGGACC CGCTCTGCCT GGAAGGCTGC GTGAGCTCAT CATTACCAAA GCCCCCCGAG 
AAGGGCAAAG ACGATGCGGT CCCTAGTTCT ACCTCTGAAA GCTCTGCGCT TTCCAGAAAG 
CGCTTCACGC TGCAGGGCTT GGCTAACCTC AAAGGTCAGA AAGCCTCTCC GACCAGTCCT 
GACAAAAAAG CTAAGCGGCA TGAAGTAAAG AGCGACCCGA CTCCCTTTGG TTTGCGAGGC 
TGGAGCAAAA CATCCCACTC ACTGGAGGCC CCTGAGGAGG ACGGAGGCTG GTCCAGTGCT 
GAGGAGCTCA TCAATTCATC CGATGCTGAG GAAGACGGAG GAGTGGGCCC TAGGAAGCTG 
GTTCCAGGTA AATACACAGT GCTGATGGAT GGTGAGAAAG GGGGCTCTGA CACCCTTGCC 
ATGAGGAGTG GAGATATGGT GGAGGTTGTG GAAGAAGGAA CTGAGGGCCT CTGGTATGTT 
CGGGACCTGA CCAGCAGCAA GGAGGGATGG GTGCCAGCCA GCAGCCTAGC CACCCTCCTG 
GGCAAATCCA GCTCAGCCCA GTGTCTGAGC AGCTCAGACT CCAGCCCAGG GTCAGCCGTG 
CTGAGCAACT CATCCAGCTG CAGCGAGGGC TACCCTACTC CCTTCTCCGA GCTCCAGGGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006253421.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253483.1 Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa37555
Clone ID Related Accession (Same CDS sequence)	XM_006253421.3
Accession Version	XM_006253421.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006253421.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACTA GCCTCCCAGG TGCTCAGGTG GTGCACACGC ATGACTCCAG GTGCGGGGTG 
GTGACACGCC CGGCAGGCTC AGTGCTTCCG CTTCCGCCTA GCGCCCCCCG CTCCCCCATC 
AACGCAAAGC GCGCCCCCCT CCCTGTGGCG CGGAGACCAA TCCTGGGAAG CCAAGGAAAG 
CGCCACAACC CCCTCCCCGC TCCAGCCGCG CCCCCTCCCC ACGCTCAGCC CCACCCGACA 
GCACCTCTGG TGCAGCGGAG GACGATCTTC TGGGGATCGG TTTTGTTGAG TCGGAGAATG 
AGGTTTTGGC TGAGAAATGA AGAGATGGCC CTAGAAGAAA TGGTGCAGAG ATTAAACGCG 
GTTTCCAAGA ACACTGATGA AATCATGCAC CAGGACATCG TCCCACTCTG TGCCGCTGAC 
ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT TACCTGTCTG GTGGGCGGGG ACAGGACGGA 
AGCCCAGTCA TCACCTTCCC AGACTACCCA GCCTTCAGCG AGATCCCGGA CAAGGAGTTC 
CAGAATGTCA TGACCTATCT CACCAGCATC CCCAGTTTGC AAGATGCTGG CATCGGGTTC 
ATCTTGGTCA TAGACCGAAG ACAGGACAAA TGGACTTCTG TGAAGGCGTC AGTCCTGCGA 
ATAGCCGCAT CATTCCCAGC CAACCTGCAG CTCGTCCTCG TTCTCCGACC CACGGGCTTC 
TTCCAGAGGA CTCTCTCGGA CATAGCCTTC AAGTTCAACA GAGATGAGTT TAAGATGAAA 
GTACCGGTCA TGATGCTAAG CTCAGTGCCA GAACTACATG GCTACATTGA CAAGTCACAG 
CTGACCGAGG ACCTTGGGGG GACCCTGGAC TACTGCCACT CCAGGTGGCT GTGCCACCGC 
ACAGCGATTG AAAGCTTCGC CCTGATGGTG AAGCAGACAG CCCAGATGCT GCAGGCCTTT 
GGGACCGAGC TGGCCGAGAC TGAGCTGCCC AACGACGTCC AGTCAACCAG CCTGGTGCTC 
AGCGCACACA CAGAGAAGAA GGCTAAAGTG AAGGAGGATC TGCAGCTGGC GCTGACGGAG 
GGGAACAGCA TCCTTGAGAG CCTCAGGGAG CCGCTGGCTG AGAGCATAGT CCACAGTGTG 
AACCAGGACC AGCTGGACAA TCAGGCCACT GTGAAGAGGC TCCTGACCCA GCTGAATGAG 
ACCGAGGCTG CCTTTGACGA GTTCTGGGCA AAGCATCAGC AGAAGCTGGA ACAGTGCCTA 
CAGCTGCGAC ATTTTGAACA AGGTTTCCGG GAGGTCAAAA CCGCCTTGGA TTCCATGTCC 
CAGAAGATAG CCGCGTTCAC AGATGTTGGC AACAGCCTGG CCCATGTGCA GCACCTCCTG 
AAGGACTTGA CCACCTTTGA GGAGAAGTCC AGCGTGGCCG TGGACAAGGC CAGAGCCCTG 
TCCCTGGAGG GGCAGCAGCT GATTGAGAAC CGGCACTATG CTGTAGACTC TATCCATCCG 
AAGTGTGAGG AACTCCAGCA CCTCTGTGAC CACTTCGCCT CTGAGGTCAC CAGGAGGCGG 
GACCTTCTCA GCAAGTCCTT GGAGCTGCAT AGCCTCCTGG AGACGTCCAT GAAGTGGAGC 
GACGAGGGCA TCTTCCTGCT GGCCTCTCAG CCTGTGGACA AATGCCAATC TCAGGATGGT 
GCGGAGGCAG CCCTGCAGGA GATCGAGAAG TTTCTGGAGA CCGGTGCAGA AAACAAGATT 
CAGGAGCTCA ATAAGATTTA CAAGGAATAT GAATGCATCC TCAACCAGGA CCTTCTGGAA 
CACGTGCAAA AAGTCTTTCA GAAGCAAGAG AGCACCGAGG AGATGTTCCA CCGCAGGCAG 
GCTAGCCTGA AGAAACTGGC AGCCAAGCAG ACACGGCCTG TGCAGCCCGT GGCCCCCCGA 
CCAGAGGCGC TAACCAAGTC CCCCTCCCCC TCGCCAGGCT CCTGGCGAAG CTCTGAGAAC 
TCCAGCTCTG AGGGCAACGC CCTCCGCAGA GGGCCCTACA GGAGAGCCAA GAGCGAAATG 
AGTGAGCCCC GGCAGGGCCG CACCAGCTCC ACAGGAGAAG AGGAGGAGAG CCTGGCCATC 
CTGCGCAGAC ACGTGATGAA TGAACTTCTG GACACGGAGC GGGCCTACGT GGAGGAGCTG 
CTCTGTGTCT TAGAGGGCTA TGCTGCAGAG ATGGACAACC CCTTGATGGC ACACCTCATC 
TCAACAGGCC TGCAAAACAA GAAGAACATT CTGTTTGGAA ACATGGAGGA AATCTATCAC 
TTCCACAACA GAATATTCCT GCGGGAGCTG GAGAGCTGCA TCGACTGCCC AGAGCTGGTG 
GGGAGATGCT TTCTGGAGAG GATGGAGGAG TTCCAGATCT ATGAGAAGTA CTGTCAGAAC 
AAGCCACGCT CCGAGAGCCT GTGGAGACAG TGCTCTGACT GTCCCTTCTT CCAGGAGTGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTC GACTCCTACC TGCTGAAGCC TGTGCAGAGG 
ATAACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAGT ACAGCAAGCA CTGTGAAGGG 
GCCGAGGACC TGCAAGAGGC ACTGAGCTCC ATCCTGGGCA TCCTCAAGGC AGTGAACGAC 
TCCATGCACC TCATAGCCAT CACTGGCTAT GATGGAAACC TTGGGGACCT GGGGAAGCTG 
CTGATGCAAG GCTCCTTCAG CGTGTGGACG GACCACAAGA AGGGCCACAC CAAGGTGAAG 
GAGCTAGCCA GGTTCAAGCC CATGCAGCGG CACCTCTTCC TTCACGAGAA GGCCGTCCTC 
TTCTGCAAGA AGAGGGAGGA GAATGGGGAG GGGTACGAGA AGGCTCCTTC CTACAGCTAC 
AAGCAGTCCT TGAATATGAC TGCTGTTGGC ATCACAGAGA ATGTGAAGGG TGACACCAAG 
AAGTTCGAGA TCTGGTACAA CGCAAGGGAG GAGGTTTACA TCATTCAGGC ACCAACTCCC 
GAGATTAAAG CAGCGTGGGT GAATGAGATT CGGAAGGTGC TGACCAGCCA ACTGCAGGCC 
TGCCGGGAAG CCAGCCAGCA CCGAGCCCTG GAGCAGTCCC ACAGCCTTCC CTTGCCCACG 
CCAGCCAGCA CCAGTCCCAC AAAAGGGAGC ACAAGAAATG TCAAGAAGCT CGAAGATCGG 
AAGACGGACC CGCTCTGCCT GGAAGGCTGC GTGAGCTCAT CATTACCAAA GCCCCCCGAG 
AAGGGCAAAG GCTGGAGCAA AACATCCCAC TCACTGGAGG CCCCTGAGGA GGACGGAGGC 
TGGTCCAGTG CTGAGGAGCT CATCAATTCA TCCGATGCTG AGGAAGACGG AGGAGTGGGC 
CCTAGGAAGC TGGTTCCAGG TAAATACACA GTGCTGATGG ATGGTGAGAA AGGGGGCTCT 
GACACCCTTG CCATGAGGAG TGGAGATATG GTGGAGGTTG TGGAAGAAGG AACTGAGGGC 
CTCTGGTATG TTCGGGACCT GACCAGCAGC AAGGAGGGAT GGGTGCCAGC CAGCAGCCTA 
GCCACCCTCC TGGGCAAATC CAGCTCAGCC CAGTGTCTGA GCAGCTCAGA CTCCAGCCCA 
GGGTCAGCCG TGCTGAGCAA CTCATCCAGC TGCAGCGAGG GCTACCCTAC TCCCTTCTCC 
GAGCTCCAGG GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253483.1
CDS	166..3840
Translation	MQTSLPGAQVVHTHDSRCGVVTRPAGSVLPLPPSAPRSPINAKRAPLPVARRPILGSQGKRHNPLPAPAAPPPHAQPHPTAPLVQRRTIFWGSVLLSRRMRFWLRNEEMALEEMVQRLNAVSKNTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALTEGNSILESLREPLAESIVHSVNQDQLDNQATVKRLLTQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTALDSMSQKIAAFTDVGNSLAHVQHLLKDLTTFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRDLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPASTSPTKGSTRNVKKLEDRKTDPLCLEGCVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPRKLVPGKYTVLMDGEKGGSDTLAMRSGDMVEVVEEGTEGLWYVRDLTSSKEGWVPASSLATLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGYPTPFSELQG

Target ORF information:

RefSeq Version	XM_006253421.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253421.3

ORF Insert Sequence:

ATGCAAACTA GCCTCCCAGG TGCTCAGGTG GTGCACACGC ATGACTCCAG GTGCGGGGTG 
GTGACACGCC CGGCAGGCTC AGTGCTTCCG CTTCCGCCTA GCGCCCCCCG CTCCCCCATC 
AACGCAAAGC GCGCCCCCCT CCCTGTGGCG CGGAGACCAA TCCTGGGAAG CCAAGGAAAG 
CGCCACAACC CCCTCCCCGC TCCAGCCGCG CCCCCTCCCC ACGCTCAGCC CCACCCGACA 
GCACCTCTGG TGCAGCGGAG GACGATCTTC TGGGGATCGG TTTTGTTGAG TCGGAGAATG 
AGGTTTTGGC TGAGAAATGA AGAGATGGCC CTAGAAGAAA TGGTGCAGAG ATTAAACGCG 
GTTTCCAAGA ACACTGATGA AATCATGCAC CAGGACATCG TCCCACTCTG TGCCGCTGAC 
ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT TACCTGTCTG GTGGGCGGGG ACAGGACGGA 
AGCCCAGTCA TCACCTTCCC AGACTACCCA GCCTTCAGCG AGATCCCGGA CAAGGAGTTC 
CAGAATGTCA TGACCTATCT CACCAGCATC CCCAGTTTGC AAGATGCTGG CATCGGGTTC 
ATCTTGGTCA TAGACCGAAG ACAGGACAAA TGGACTTCTG TGAAGGCGTC AGTCCTGCGA 
ATAGCCGCAT CATTCCCAGC CAACCTGCAG CTCGTCCTCG TTCTCCGACC CACGGGCTTC 
TTCCAGAGGA CTCTCTCGGA CATAGCCTTC AAGTTCAACA GAGATGAGTT TAAGATGAAA 
GTACCGGTCA TGATGCTAAG CTCAGTGCCA GAACTACATG GCTACATTGA CAAGTCACAG 
CTGACCGAGG ACCTTGGGGG GACCCTGGAC TACTGCCACT CCAGGTGGCT GTGCCACCGC 
ACAGCGATTG AAAGCTTCGC CCTGATGGTG AAGCAGACAG CCCAGATGCT GCAGGCCTTT 
GGGACCGAGC TGGCCGAGAC TGAGCTGCCC AACGACGTCC AGTCAACCAG CCTGGTGCTC 
AGCGCACACA CAGAGAAGAA GGCTAAAGTG AAGGAGGATC TGCAGCTGGC GCTGACGGAG 
GGGAACAGCA TCCTTGAGAG CCTCAGGGAG CCGCTGGCTG AGAGCATAGT CCACAGTGTG 
AACCAGGACC AGCTGGACAA TCAGGCCACT GTGAAGAGGC TCCTGACCCA GCTGAATGAG 
ACCGAGGCTG CCTTTGACGA GTTCTGGGCA AAGCATCAGC AGAAGCTGGA ACAGTGCCTA 
CAGCTGCGAC ATTTTGAACA AGGTTTCCGG GAGGTCAAAA CCGCCTTGGA TTCCATGTCC 
CAGAAGATAG CCGCGTTCAC AGATGTTGGC AACAGCCTGG CCCATGTGCA GCACCTCCTG 
AAGGACTTGA CCACCTTTGA GGAGAAGTCC AGCGTGGCCG TGGACAAGGC CAGAGCCCTG 
TCCCTGGAGG GGCAGCAGCT GATTGAGAAC CGGCACTATG CTGTAGACTC TATCCATCCG 
AAGTGTGAGG AACTCCAGCA CCTCTGTGAC CACTTCGCCT CTGAGGTCAC CAGGAGGCGG 
GACCTTCTCA GCAAGTCCTT GGAGCTGCAT AGCCTCCTGG AGACGTCCAT GAAGTGGAGC 
GACGAGGGCA TCTTCCTGCT GGCCTCTCAG CCTGTGGACA AATGCCAATC TCAGGATGGT 
GCGGAGGCAG CCCTGCAGGA GATCGAGAAG TTTCTGGAGA CCGGTGCAGA AAACAAGATT 
CAGGAGCTCA ATAAGATTTA CAAGGAATAT GAATGCATCC TCAACCAGGA CCTTCTGGAA 
CACGTGCAAA AAGTCTTTCA GAAGCAAGAG AGCACCGAGG AGATGTTCCA CCGCAGGCAG 
GCTAGCCTGA AGAAACTGGC AGCCAAGCAG ACACGGCCTG TGCAGCCCGT GGCCCCCCGA 
CCAGAGGCGC TAACCAAGTC CCCCTCCCCC TCGCCAGGCT CCTGGCGAAG CTCTGAGAAC 
TCCAGCTCTG AGGGCAACGC CCTCCGCAGA GGGCCCTACA GGAGAGCCAA GAGCGAAATG 
AGTGAGCCCC GGCAGGGCCG CACCAGCTCC ACAGGAGAAG AGGAGGAGAG CCTGGCCATC 
CTGCGCAGAC ACGTGATGAA TGAACTTCTG GACACGGAGC GGGCCTACGT GGAGGAGCTG 
CTCTGTGTCT TAGAGGGCTA TGCTGCAGAG ATGGACAACC CCTTGATGGC ACACCTCATC 
TCAACAGGCC TGCAAAACAA GAAGAACATT CTGTTTGGAA ACATGGAGGA AATCTATCAC 
TTCCACAACA GAATATTCCT GCGGGAGCTG GAGAGCTGCA TCGACTGCCC AGAGCTGGTG 
GGGAGATGCT TTCTGGAGAG GATGGAGGAG TTCCAGATCT ATGAGAAGTA CTGTCAGAAC 
AAGCCACGCT CCGAGAGCCT GTGGAGACAG TGCTCTGACT GTCCCTTCTT CCAGGAGTGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTC GACTCCTACC TGCTGAAGCC TGTGCAGAGG 
ATAACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAGT ACAGCAAGCA CTGTGAAGGG 
GCCGAGGACC TGCAAGAGGC ACTGAGCTCC ATCCTGGGCA TCCTCAAGGC AGTGAACGAC 
TCCATGCACC TCATAGCCAT CACTGGCTAT GATGGAAACC TTGGGGACCT GGGGAAGCTG 
CTGATGCAAG GCTCCTTCAG CGTGTGGACG GACCACAAGA AGGGCCACAC CAAGGTGAAG 
GAGCTAGCCA GGTTCAAGCC CATGCAGCGG CACCTCTTCC TTCACGAGAA GGCCGTCCTC 
TTCTGCAAGA AGAGGGAGGA GAATGGGGAG GGGTACGAGA AGGCTCCTTC CTACAGCTAC 
AAGCAGTCCT TGAATATGAC TGCTGTTGGC ATCACAGAGA ATGTGAAGGG TGACACCAAG 
AAGTTCGAGA TCTGGTACAA CGCAAGGGAG GAGGTTTACA TCATTCAGGC ACCAACTCCC 
GAGATTAAAG CAGCGTGGGT GAATGAGATT CGGAAGGTGC TGACCAGCCA ACTGCAGGCC 
TGCCGGGAAG CCAGCCAGCA CCGAGCCCTG GAGCAGTCCC ACAGCCTTCC CTTGCCCACG 
CCAGCCAGCA CCAGTCCCAC AAAAGGGAGC ACAAGAAATG TCAAGAAGCT CGAAGATCGG 
AAGACGGACC CGCTCTGCCT GGAAGGCTGC GTGAGCTCAT CATTACCAAA GCCCCCCGAG 
AAGGGCAAAG GCTGGAGCAA AACATCCCAC TCACTGGAGG CCCCTGAGGA GGACGGAGGC 
TGGTCCAGTG CTGAGGAGCT CATCAATTCA TCCGATGCTG AGGAAGACGG AGGAGTGGGC 
CCTAGGAAGC TGGTTCCAGG TAAATACACA GTGCTGATGG ATGGTGAGAA AGGGGGCTCT 
GACACCCTTG CCATGAGGAG TGGAGATATG GTGGAGGTTG TGGAAGAAGG AACTGAGGGC 
CTCTGGTATG TTCGGGACCT GACCAGCAGC AAGGAGGGAT GGGTGCCAGC CAGCAGCCTA 
GCCACCCTCC TGGGCAAATC CAGCTCAGCC CAGTGTCTGA GCAGCTCAGA CTCCAGCCCA 
GGGTCAGCCG TGCTGAGCAA CTCATCCAGC TGCAGCGAGG GCTACCCTAC TCCCTTCTCC 
GAGCTCCAGG GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006253423.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253485.1 Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X4, mRNA.

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CloneID	ORa37557
Clone ID Related Accession (Same CDS sequence)	XM_006253423.3
Accession Version	XM_006253423.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide exchange factor DBS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006253423.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA AGGGTGCGTC CCGGGGGGCC GGGCGGCGGT TCTGGTCACT ACCAAGGAAG 
CGTCGGGGAG CACCAGGGCG CCACAAGCCA GATGAAATCA TGCACCAGGA CATCGTCCCA 
CTCTGTGCCG CTGACATCCA GGAGCAGCTG AAGAAGCGCT TTGCTTACCT GTCTGGTGGG 
CGGGGACAGG ACGGAAGCCC AGTCATCACC TTCCCAGACT ACCCAGCCTT CAGCGAGATC 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TATCTCACCA GCATCCCCAG TTTGCAAGAT 
GCTGGCATCG GGTTCATCTT GGTCATAGAC CGAAGACAGG ACAAATGGAC TTCTGTGAAG 
GCGTCAGTCC TGCGAATAGC CGCATCATTC CCAGCCAACC TGCAGCTCGT CCTCGTTCTC 
CGACCCACGG GCTTCTTCCA GAGGACTCTC TCGGACATAG CCTTCAAGTT CAACAGAGAT 
GAGTTTAAGA TGAAAGTACC GGTCATGATG CTAAGCTCAG TGCCAGAACT ACATGGCTAC 
ATTGACAAGT CACAGCTGAC CGAGGACCTT GGGGGGACCC TGGACTACTG CCACTCCAGG 
TGGCTGTGCC ACCGCACAGC GATTGAAAGC TTCGCCCTGA TGGTGAAGCA GACAGCCCAG 
ATGCTGCAGG CCTTTGGGAC CGAGCTGGCC GAGACTGAGC TGCCCAACGA CGTCCAGTCA 
ACCAGCCTGG TGCTCAGCGC ACACACAGAG AAGAAGGCTA AAGTGAAGGA GGATCTGCAG 
CTGGCGCTGA CGGAGGGGAA CAGCATCCTT GAGAGCCTCA GGGAGCCGCT GGCTGAGAGC 
ATAGTCCACA GTGTGAACCA GGACCAGCTG GACAATCAGG CCACTGTGAA GAGGCTCCTG 
ACCCAGCTGA ATGAGACCGA GGCTGCCTTT GACGAGTTCT GGGCAAAGCA TCAGCAGAAG 
CTGGAACAGT GCCTACAGCT GCGACATTTT GAACAAGGTT TCCGGGAGGT CAAAACCGCC 
TTGGATTCCA TGTCCCAGAA GATAGCCGCG TTCACAGATG TTGGCAACAG CCTGGCCCAT 
GTGCAGCACC TCCTGAAGGA CTTGACCACC TTTGAGGAGA AGTCCAGCGT GGCCGTGGAC 
AAGGCCAGAG CCCTGTCCCT GGAGGGGCAG CAGCTGATTG AGAACCGGCA CTATGCTGTA 
GACTCTATCC ATCCGAAGTG TGAGGAACTC CAGCACCTCT GTGACCACTT CGCCTCTGAG 
GTCACCAGGA GGCGGGACCT TCTCAGCAAG TCCTTGGAGC TGCATAGCCT CCTGGAGACG 
TCCATGAAGT GGAGCGACGA GGGCATCTTC CTGCTGGCCT CTCAGCCTGT GGACAAATGC 
CAATCTCAGG ATGGTGCGGA GGCAGCCCTG CAGGAGATCG AGAAGTTTCT GGAGACCGGT 
GCAGAAAACA AGATTCAGGA GCTCAATAAG ATTTACAAGG AATATGAATG CATCCTCAAC 
CAGGACCTTC TGGAACACGT GCAAAAAGTC TTTCAGAAGC AAGAGAGCAC CGAGGAGATG 
TTCCACCGCA GGCAGGCTAG CCTGAAGAAA CTGGCAGCCA AGCAGACACG GCCTGTGCAG 
CCCGTGGCCC CCCGACCAGA GGCGCTAACC AAGTCCCCCT CCCCCTCGCC AGGCTCCTGG 
CGAAGCTCTG AGAACTCCAG CTCTGAGGGC AACGCCCTCC GCAGAGGGCC CTACAGGAGA 
GCCAAGAGCG AAATGAGTGA GCCCCGGCAG GGCCGCACCA GCTCCACAGG AGAAGAGGAG 
GAGAGCCTGG CCATCCTGCG CAGACACGTG ATGAATGAAC TTCTGGACAC GGAGCGGGCC 
TACGTGGAGG AGCTGCTCTG TGTCTTAGAG GGCTATGCTG CAGAGATGGA CAACCCCTTG 
ATGGCACACC TCATCTCAAC AGGCCTGCAA AACAAGAAGA ACATTCTGTT TGGAAACATG 
GAGGAAATCT ATCACTTCCA CAACAGAATA TTCCTGCGGG AGCTGGAGAG CTGCATCGAC 
TGCCCAGAGC TGGTGGGGAG ATGCTTTCTG GAGAGGATGG AGGAGTTCCA GATCTATGAG 
AAGTACTGTC AGAACAAGCC ACGCTCCGAG AGCCTGTGGA GACAGTGCTC TGACTGTCCC 
TTCTTCCAGG AGTGCCAGAA GAAGCTGGAC CACAAGCTGA GCCTCGACTC CTACCTGCTG 
AAGCCTGTGC AGAGGATAAC CAAGTACCAG CTGCTGCTCA AGGAAATGCT GAAGTACAGC 
AAGCACTGTG AAGGGGCCGA GGACCTGCAA GAGGCACTGA GCTCCATCCT GGGCATCCTC 
AAGGCAGTGA ACGACTCCAT GCACCTCATA GCCATCACTG GCTATGATGG AAACCTTGGG 
GACCTGGGGA AGCTGCTGAT GCAAGGCTCC TTCAGCGTGT GGACGGACCA CAAGAAGGGC 
CACACCAAGG TGAAGGAGCT AGCCAGGTTC AAGCCCATGC AGCGGCACCT CTTCCTTCAC 
GAGAAGGCCG TCCTCTTCTG CAAGAAGAGG GAGGAGAATG GGGAGGGGTA CGAGAAGGCT 
CCTTCCTACA GCTACAAGCA GTCCTTGAAT ATGACTGCTG TTGGCATCAC AGAGAATGTG 
AAGGGTGACA CCAAGAAGTT CGAGATCTGG TACAACGCAA GGGAGGAGGT TTACATCATT 
CAGGCACCAA CTCCCGAGAT TAAAGCAGCG TGGGTGAATG AGATTCGGAA GGTGCTGACC 
AGCCAACTGC AGGCCTGCCG GGAAGCCAGC CAGCACCGAG CCCTGGAGCA GTCCCACAGC 
CTTCCCTTGC CCACGCCAGC CAGCACCAGT CCCACAAAAG GGAGCACAAG AAATGTCAAG 
AAGCTCGAAG ATCGGAAGAC GGACCCGCTC TGCCTGGAAG GCTGCGTGAG CTCATCATTA 
CCAAAGCCCC CCGAGAAGGG CAAAGACGAT GCGGTCCCTA GTTCTACCTC TGAAAGCTCT 
GCGCTTTCCA GAAAGCGCTT CACGCTGCAG GGCTTGGCTA ACCTCAAAGG TCAGAAAGCC 
TCTCCGACCA GTCCTGACAA AAAAGCTAAG CGGCATGAAG TAAAGAGCGA CCCGACTCCC 
TTTGGTTTGC GAGGCTGGAG CAAAACATCC CACTCACTGG AGGCCCCTGA GGAGGACGGA 
GGCTGGTCCA GTGCTGAGGA GCTCATCAAT TCATCCGATG CTGAGGAAGA CGGAGGAGTG 
GGCCCTAGGA AGCTGGTTCC AGGTAAATAC ACAGTGCTGA TGGATGGTGA GAAAGGGGGC 
TCTGACACCC TTGCCATGAG GAGTGGAGAT ATGGTGGAGG TTGTGGAAGA AGGAACTGAG 
GGCCTCTGGT ATGTTCGGGA CCTGACCAGC AGCAAGGAGG GATGGGTGCC AGCCAGCAGC 
CTAGCCACCC TCCTGGGCAA ATCCAGCTCA GCCCAGTGTC TGAGCAGCTC AGACTCCAGC 
CCAGGGTCAG CCGTGCTGAG CAACTCATCC AGCTGCAGCG AGGGCTACCC TACTCCCTTC 
TCCGAGCTCC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253485.1
CDS	474..4031
Translation	MSEKGASRGAGRRFWSLPRKRRGAPGRHKPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALTEGNSILESLREPLAESIVHSVNQDQLDNQATVKRLLTQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTALDSMSQKIAAFTDVGNSLAHVQHLLKDLTTFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRDLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPASTSPTKGSTRNVKKLEDRKTDPLCLEGCVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPRKLVPGKYTVLMDGEKGGSDTLAMRSGDMVEVVEEGTEGLWYVRDLTSSKEGWVPASSLATLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGYPTPFSELQG

Target ORF information:

RefSeq Version	XM_006253423.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253423.3

ORF Insert Sequence:

ATGTCAGAAA AGGGTGCGTC CCGGGGGGCC GGGCGGCGGT TCTGGTCACT ACCAAGGAAG 
CGTCGGGGAG CACCAGGGCG CCACAAGCCA GATGAAATCA TGCACCAGGA CATCGTCCCA 
CTCTGTGCCG CTGACATCCA GGAGCAGCTG AAGAAGCGCT TTGCTTACCT GTCTGGTGGG 
CGGGGACAGG ACGGAAGCCC AGTCATCACC TTCCCAGACT ACCCAGCCTT CAGCGAGATC 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TATCTCACCA GCATCCCCAG TTTGCAAGAT 
GCTGGCATCG GGTTCATCTT GGTCATAGAC CGAAGACAGG ACAAATGGAC TTCTGTGAAG 
GCGTCAGTCC TGCGAATAGC CGCATCATTC CCAGCCAACC TGCAGCTCGT CCTCGTTCTC 
CGACCCACGG GCTTCTTCCA GAGGACTCTC TCGGACATAG CCTTCAAGTT CAACAGAGAT 
GAGTTTAAGA TGAAAGTACC GGTCATGATG CTAAGCTCAG TGCCAGAACT ACATGGCTAC 
ATTGACAAGT CACAGCTGAC CGAGGACCTT GGGGGGACCC TGGACTACTG CCACTCCAGG 
TGGCTGTGCC ACCGCACAGC GATTGAAAGC TTCGCCCTGA TGGTGAAGCA GACAGCCCAG 
ATGCTGCAGG CCTTTGGGAC CGAGCTGGCC GAGACTGAGC TGCCCAACGA CGTCCAGTCA 
ACCAGCCTGG TGCTCAGCGC ACACACAGAG AAGAAGGCTA AAGTGAAGGA GGATCTGCAG 
CTGGCGCTGA CGGAGGGGAA CAGCATCCTT GAGAGCCTCA GGGAGCCGCT GGCTGAGAGC 
ATAGTCCACA GTGTGAACCA GGACCAGCTG GACAATCAGG CCACTGTGAA GAGGCTCCTG 
ACCCAGCTGA ATGAGACCGA GGCTGCCTTT GACGAGTTCT GGGCAAAGCA TCAGCAGAAG 
CTGGAACAGT GCCTACAGCT GCGACATTTT GAACAAGGTT TCCGGGAGGT CAAAACCGCC 
TTGGATTCCA TGTCCCAGAA GATAGCCGCG TTCACAGATG TTGGCAACAG CCTGGCCCAT 
GTGCAGCACC TCCTGAAGGA CTTGACCACC TTTGAGGAGA AGTCCAGCGT GGCCGTGGAC 
AAGGCCAGAG CCCTGTCCCT GGAGGGGCAG CAGCTGATTG AGAACCGGCA CTATGCTGTA 
GACTCTATCC ATCCGAAGTG TGAGGAACTC CAGCACCTCT GTGACCACTT CGCCTCTGAG 
GTCACCAGGA GGCGGGACCT TCTCAGCAAG TCCTTGGAGC TGCATAGCCT CCTGGAGACG 
TCCATGAAGT GGAGCGACGA GGGCATCTTC CTGCTGGCCT CTCAGCCTGT GGACAAATGC 
CAATCTCAGG ATGGTGCGGA GGCAGCCCTG CAGGAGATCG AGAAGTTTCT GGAGACCGGT 
GCAGAAAACA AGATTCAGGA GCTCAATAAG ATTTACAAGG AATATGAATG CATCCTCAAC 
CAGGACCTTC TGGAACACGT GCAAAAAGTC TTTCAGAAGC AAGAGAGCAC CGAGGAGATG 
TTCCACCGCA GGCAGGCTAG CCTGAAGAAA CTGGCAGCCA AGCAGACACG GCCTGTGCAG 
CCCGTGGCCC CCCGACCAGA GGCGCTAACC AAGTCCCCCT CCCCCTCGCC AGGCTCCTGG 
CGAAGCTCTG AGAACTCCAG CTCTGAGGGC AACGCCCTCC GCAGAGGGCC CTACAGGAGA 
GCCAAGAGCG AAATGAGTGA GCCCCGGCAG GGCCGCACCA GCTCCACAGG AGAAGAGGAG 
GAGAGCCTGG CCATCCTGCG CAGACACGTG ATGAATGAAC TTCTGGACAC GGAGCGGGCC 
TACGTGGAGG AGCTGCTCTG TGTCTTAGAG GGCTATGCTG CAGAGATGGA CAACCCCTTG 
ATGGCACACC TCATCTCAAC AGGCCTGCAA AACAAGAAGA ACATTCTGTT TGGAAACATG 
GAGGAAATCT ATCACTTCCA CAACAGAATA TTCCTGCGGG AGCTGGAGAG CTGCATCGAC 
TGCCCAGAGC TGGTGGGGAG ATGCTTTCTG GAGAGGATGG AGGAGTTCCA GATCTATGAG 
AAGTACTGTC AGAACAAGCC ACGCTCCGAG AGCCTGTGGA GACAGTGCTC TGACTGTCCC 
TTCTTCCAGG AGTGCCAGAA GAAGCTGGAC CACAAGCTGA GCCTCGACTC CTACCTGCTG 
AAGCCTGTGC AGAGGATAAC CAAGTACCAG CTGCTGCTCA AGGAAATGCT GAAGTACAGC 
AAGCACTGTG AAGGGGCCGA GGACCTGCAA GAGGCACTGA GCTCCATCCT GGGCATCCTC 
AAGGCAGTGA ACGACTCCAT GCACCTCATA GCCATCACTG GCTATGATGG AAACCTTGGG 
GACCTGGGGA AGCTGCTGAT GCAAGGCTCC TTCAGCGTGT GGACGGACCA CAAGAAGGGC 
CACACCAAGG TGAAGGAGCT AGCCAGGTTC AAGCCCATGC AGCGGCACCT CTTCCTTCAC 
GAGAAGGCCG TCCTCTTCTG CAAGAAGAGG GAGGAGAATG GGGAGGGGTA CGAGAAGGCT 
CCTTCCTACA GCTACAAGCA GTCCTTGAAT ATGACTGCTG TTGGCATCAC AGAGAATGTG 
AAGGGTGACA CCAAGAAGTT CGAGATCTGG TACAACGCAA GGGAGGAGGT TTACATCATT 
CAGGCACCAA CTCCCGAGAT TAAAGCAGCG TGGGTGAATG AGATTCGGAA GGTGCTGACC 
AGCCAACTGC AGGCCTGCCG GGAAGCCAGC CAGCACCGAG CCCTGGAGCA GTCCCACAGC 
CTTCCCTTGC CCACGCCAGC CAGCACCAGT CCCACAAAAG GGAGCACAAG AAATGTCAAG 
AAGCTCGAAG ATCGGAAGAC GGACCCGCTC TGCCTGGAAG GCTGCGTGAG CTCATCATTA 
CCAAAGCCCC CCGAGAAGGG CAAAGACGAT GCGGTCCCTA GTTCTACCTC TGAAAGCTCT 
GCGCTTTCCA GAAAGCGCTT CACGCTGCAG GGCTTGGCTA ACCTCAAAGG TCAGAAAGCC 
TCTCCGACCA GTCCTGACAA AAAAGCTAAG CGGCATGAAG TAAAGAGCGA CCCGACTCCC 
TTTGGTTTGC GAGGCTGGAG CAAAACATCC CACTCACTGG AGGCCCCTGA GGAGGACGGA 
GGCTGGTCCA GTGCTGAGGA GCTCATCAAT TCATCCGATG CTGAGGAAGA CGGAGGAGTG 
GGCCCTAGGA AGCTGGTTCC AGGTAAATAC ACAGTGCTGA TGGATGGTGA GAAAGGGGGC 
TCTGACACCC TTGCCATGAG GAGTGGAGAT ATGGTGGAGG TTGTGGAAGA AGGAACTGAG 
GGCCTCTGGT ATGTTCGGGA CCTGACCAGC AGCAAGGAGG GATGGGTGCC AGCCAGCAGC 
CTAGCCACCC TCCTGGGCAA ATCCAGCTCA GCCCAGTGTC TGAGCAGCTC AGACTCCAGC 
CCAGGGTCAG CCGTGCTGAG CAACTCATCC AGCTGCAGCG AGGGCTACCC TACTCCCTTC 
TCCGAGCTCC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_006253424.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253486.1 Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X5, mRNA.

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CloneID	ORa37558
Clone ID Related Accession (Same CDS sequence)	XM_006253424.3
Accession Version	XM_006253424.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006253424.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTGA GCCTCATCTC CGTGCTGTCC CAGGACGTCA CAGCCCTGTG GCTCCTGCTG 
AAGACAAGTG CAGATGAAAT CATGCACCAG GACATCGTCC CACTCTGTGC CGCTGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGACA GGACGGAAGC 
CCAGTCATCA CCTTCCCAGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT CGGGTTCATC 
TTGGTCATAG ACCGAAGACA GGACAAATGG ACTTCTGTGA AGGCGTCAGT CCTGCGAATA 
GCCGCATCAT TCCCAGCCAA CCTGCAGCTC GTCCTCGTTC TCCGACCCAC GGGCTTCTTC 
CAGAGGACTC TCTCGGACAT AGCCTTCAAG TTCAACAGAG ATGAGTTTAA GATGAAAGTA 
CCGGTCATGA TGCTAAGCTC AGTGCCAGAA CTACATGGCT ACATTGACAA GTCACAGCTG 
ACCGAGGACC TTGGGGGGAC CCTGGACTAC TGCCACTCCA GGTGGCTGTG CCACCGCACA 
GCGATTGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCCC AGATGCTGCA GGCCTTTGGG 
ACCGAGCTGG CCGAGACTGA GCTGCCCAAC GACGTCCAGT CAACCAGCCT GGTGCTCAGC 
GCACACACAG AGAAGAAGGC TAAAGTGAAG GAGGATCTGC AGCTGGCGCT GACGGAGGGG 
AACAGCATCC TTGAGAGCCT CAGGGAGCCG CTGGCTGAGA GCATAGTCCA CAGTGTGAAC 
CAGGACCAGC TGGACAATCA GGCCACTGTG AAGAGGCTCC TGACCCAGCT GAATGAGACC 
GAGGCTGCCT TTGACGAGTT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTACAG 
CTGCGACATT TTGAACAAGG TTTCCGGGAG GTCAAAACCG CCTTGGATTC CATGTCCCAG 
AAGATAGCCG CGTTCACAGA TGTTGGCAAC AGCCTGGCCC ATGTGCAGCA CCTCCTGAAG 
GACTTGACCA CCTTTGAGGA GAAGTCCAGC GTGGCCGTGG ACAAGGCCAG AGCCCTGTCC 
CTGGAGGGGC AGCAGCTGAT TGAGAACCGG CACTATGCTG TAGACTCTAT CCATCCGAAG 
TGTGAGGAAC TCCAGCACCT CTGTGACCAC TTCGCCTCTG AGGTCACCAG GAGGCGGGAC 
CTTCTCAGCA AGTCCTTGGA GCTGCATAGC CTCCTGGAGA CGTCCATGAA GTGGAGCGAC 
GAGGGCATCT TCCTGCTGGC CTCTCAGCCT GTGGACAAAT GCCAATCTCA GGATGGTGCG 
GAGGCAGCCC TGCAGGAGAT CGAGAAGTTT CTGGAGACCG GTGCAGAAAA CAAGATTCAG 
GAGCTCAATA AGATTTACAA GGAATATGAA TGCATCCTCA ACCAGGACCT TCTGGAACAC 
GTGCAAAAAG TCTTTCAGAA GCAAGAGAGC ACCGAGGAGA TGTTCCACCG CAGGCAGGCT 
AGCCTGAAGA AACTGGCAGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCCCGACCA 
GAGGCGCTAA CCAAGTCCCC CTCCCCCTCG CCAGGCTCCT GGCGAAGCTC TGAGAACTCC 
AGCTCTGAGG GCAACGCCCT CCGCAGAGGG CCCTACAGGA GAGCCAAGAG CGAAATGAGT 
GAGCCCCGGC AGGGCCGCAC CAGCTCCACA GGAGAAGAGG AGGAGAGCCT GGCCATCCTG 
CGCAGACACG TGATGAATGA ACTTCTGGAC ACGGAGCGGG CCTACGTGGA GGAGCTGCTC 
TGTGTCTTAG AGGGCTATGC TGCAGAGATG GACAACCCCT TGATGGCACA CCTCATCTCA 
ACAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAAAT CTATCACTTC 
CACAACAGAA TATTCCTGCG GGAGCTGGAG AGCTGCATCG ACTGCCCAGA GCTGGTGGGG 
AGATGCTTTC TGGAGAGGAT GGAGGAGTTC CAGATCTATG AGAAGTACTG TCAGAACAAG 
CCACGCTCCG AGAGCCTGTG GAGACAGTGC TCTGACTGTC CCTTCTTCCA GGAGTGCCAG 
AAGAAGCTGG ACCACAAGCT GAGCCTCGAC TCCTACCTGC TGAAGCCTGT GCAGAGGATA 
ACCAAGTACC AGCTGCTGCT CAAGGAAATG CTGAAGTACA GCAAGCACTG TGAAGGGGCC 
GAGGACCTGC AAGAGGCACT GAGCTCCATC CTGGGCATCC TCAAGGCAGT GAACGACTCC 
ATGCACCTCA TAGCCATCAC TGGCTATGAT GGAAACCTTG GGGACCTGGG GAAGCTGCTG 
ATGCAAGGCT CCTTCAGCGT GTGGACGGAC CACAAGAAGG GCCACACCAA GGTGAAGGAG 
CTAGCCAGGT TCAAGCCCAT GCAGCGGCAC CTCTTCCTTC ACGAGAAGGC CGTCCTCTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGG TACGAGAAGG CTCCTTCCTA CAGCTACAAG 
CAGTCCTTGA ATATGACTGC TGTTGGCATC ACAGAGAATG TGAAGGGTGA CACCAAGAAG 
TTCGAGATCT GGTACAACGC AAGGGAGGAG GTTTACATCA TTCAGGCACC AACTCCCGAG 
ATTAAAGCAG CGTGGGTGAA TGAGATTCGG AAGGTGCTGA CCAGCCAACT GCAGGCCTGC 
CGGGAAGCCA GCCAGCACCG AGCCCTGGAG CAGTCCCACA GCCTTCCCTT GCCCACGCCA 
GCCAGCACCA GTCCCACAAA AGGGAGCACA AGAAATGTCA AGAAGCTCGA AGATCGGAAG 
ACGGACCCGC TCTGCCTGGA AGGCTGCGTG AGCTCATCAT TACCAAAGCC CCCCGAGAAG 
GGCAAAGACG ATGCGGTCCC TAGTTCTACC TCTGAAAGCT CTGCGCTTTC CAGAAAGCGC 
TTCACGCTGC AGGGCTTGGC TAACCTCAAA GGTCAGAAAG CCTCTCCGAC CAGTCCTGAC 
AAAAAAGCTA AGCGGCATGA AGTAAAGAGC GACCCGACTC CCTTTGGTTT GCGAGGCTGG 
AGCAAAACAT CCCACTCACT GGAGGCCCCT GAGGAGGACG GAGGCTGGTC CAGTGCTGAG 
GAGCTCATCA ATTCATCCGA TGCTGAGGAA GACGGAGGAG TGGGCCCTAG GAAGCTGGTT 
CCAGGTAAAT ACACAGTGCT GATGGATGGT GAGAAAGGGG GCTCTGACAC CCTTGCCATG 
AGGAGTGGAG ATATGGTGGA GGTTGTGGAA GAAGGAACTG AGGGCCTCTG GTATGTTCGG 
GACCTGACCA GCAGCAAGGA GGGATGGGTG CCAGCCAGCA GCCTAGCCAC CCTCCTGGGC 
AAATCCAGCT CAGCCCAGTG TCTGAGCAGC TCAGACTCCA GCCCAGGGTC AGCCGTGCTG 
AGCAACTCAT CCAGCTGCAG CGAGGGCTAC CCTACTCCCT TCTCCGAGCT CCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253486.1
CDS	2102..5641
Translation	MTLSLISVLSQDVTALWLLLKTSADEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALTEGNSILESLREPLAESIVHSVNQDQLDNQATVKRLLTQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTALDSMSQKIAAFTDVGNSLAHVQHLLKDLTTFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRDLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPASTSPTKGSTRNVKKLEDRKTDPLCLEGCVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPRKLVPGKYTVLMDGEKGGSDTLAMRSGDMVEVVEEGTEGLWYVRDLTSSKEGWVPASSLATLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGYPTPFSELQG

Target ORF information:

RefSeq Version	XM_006253424.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MCF.2 cell line derived transforming sequence-like (Mcf2l), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253424.3

ORF Insert Sequence:

ATGACTTTGA GCCTCATCTC CGTGCTGTCC CAGGACGTCA CAGCCCTGTG GCTCCTGCTG 
AAGACAAGTG CAGATGAAAT CATGCACCAG GACATCGTCC CACTCTGTGC CGCTGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGACA GGACGGAAGC 
CCAGTCATCA CCTTCCCAGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT CGGGTTCATC 
TTGGTCATAG ACCGAAGACA GGACAAATGG ACTTCTGTGA AGGCGTCAGT CCTGCGAATA 
GCCGCATCAT TCCCAGCCAA CCTGCAGCTC GTCCTCGTTC TCCGACCCAC GGGCTTCTTC 
CAGAGGACTC TCTCGGACAT AGCCTTCAAG TTCAACAGAG ATGAGTTTAA GATGAAAGTA 
CCGGTCATGA TGCTAAGCTC AGTGCCAGAA CTACATGGCT ACATTGACAA GTCACAGCTG 
ACCGAGGACC TTGGGGGGAC CCTGGACTAC TGCCACTCCA GGTGGCTGTG CCACCGCACA 
GCGATTGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCCC AGATGCTGCA GGCCTTTGGG 
ACCGAGCTGG CCGAGACTGA GCTGCCCAAC GACGTCCAGT CAACCAGCCT GGTGCTCAGC 
GCACACACAG AGAAGAAGGC TAAAGTGAAG GAGGATCTGC AGCTGGCGCT GACGGAGGGG 
AACAGCATCC TTGAGAGCCT CAGGGAGCCG CTGGCTGAGA GCATAGTCCA CAGTGTGAAC 
CAGGACCAGC TGGACAATCA GGCCACTGTG AAGAGGCTCC TGACCCAGCT GAATGAGACC 
GAGGCTGCCT TTGACGAGTT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTACAG 
CTGCGACATT TTGAACAAGG TTTCCGGGAG GTCAAAACCG CCTTGGATTC CATGTCCCAG 
AAGATAGCCG CGTTCACAGA TGTTGGCAAC AGCCTGGCCC ATGTGCAGCA CCTCCTGAAG 
GACTTGACCA CCTTTGAGGA GAAGTCCAGC GTGGCCGTGG ACAAGGCCAG AGCCCTGTCC 
CTGGAGGGGC AGCAGCTGAT TGAGAACCGG CACTATGCTG TAGACTCTAT CCATCCGAAG 
TGTGAGGAAC TCCAGCACCT CTGTGACCAC TTCGCCTCTG AGGTCACCAG GAGGCGGGAC 
CTTCTCAGCA AGTCCTTGGA GCTGCATAGC CTCCTGGAGA CGTCCATGAA GTGGAGCGAC 
GAGGGCATCT TCCTGCTGGC CTCTCAGCCT GTGGACAAAT GCCAATCTCA GGATGGTGCG 
GAGGCAGCCC TGCAGGAGAT CGAGAAGTTT CTGGAGACCG GTGCAGAAAA CAAGATTCAG 
GAGCTCAATA AGATTTACAA GGAATATGAA TGCATCCTCA ACCAGGACCT TCTGGAACAC 
GTGCAAAAAG TCTTTCAGAA GCAAGAGAGC ACCGAGGAGA TGTTCCACCG CAGGCAGGCT 
AGCCTGAAGA AACTGGCAGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCCCGACCA 
GAGGCGCTAA CCAAGTCCCC CTCCCCCTCG CCAGGCTCCT GGCGAAGCTC TGAGAACTCC 
AGCTCTGAGG GCAACGCCCT CCGCAGAGGG CCCTACAGGA GAGCCAAGAG CGAAATGAGT 
GAGCCCCGGC AGGGCCGCAC CAGCTCCACA GGAGAAGAGG AGGAGAGCCT GGCCATCCTG 
CGCAGACACG TGATGAATGA ACTTCTGGAC ACGGAGCGGG CCTACGTGGA GGAGCTGCTC 
TGTGTCTTAG AGGGCTATGC TGCAGAGATG GACAACCCCT TGATGGCACA CCTCATCTCA 
ACAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAAAT CTATCACTTC 
CACAACAGAA TATTCCTGCG GGAGCTGGAG AGCTGCATCG ACTGCCCAGA GCTGGTGGGG 
AGATGCTTTC TGGAGAGGAT GGAGGAGTTC CAGATCTATG AGAAGTACTG TCAGAACAAG 
CCACGCTCCG AGAGCCTGTG GAGACAGTGC TCTGACTGTC CCTTCTTCCA GGAGTGCCAG 
AAGAAGCTGG ACCACAAGCT GAGCCTCGAC TCCTACCTGC TGAAGCCTGT GCAGAGGATA 
ACCAAGTACC AGCTGCTGCT CAAGGAAATG CTGAAGTACA GCAAGCACTG TGAAGGGGCC 
GAGGACCTGC AAGAGGCACT GAGCTCCATC CTGGGCATCC TCAAGGCAGT GAACGACTCC 
ATGCACCTCA TAGCCATCAC TGGCTATGAT GGAAACCTTG GGGACCTGGG GAAGCTGCTG 
ATGCAAGGCT CCTTCAGCGT GTGGACGGAC CACAAGAAGG GCCACACCAA GGTGAAGGAG 
CTAGCCAGGT TCAAGCCCAT GCAGCGGCAC CTCTTCCTTC ACGAGAAGGC CGTCCTCTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGG TACGAGAAGG CTCCTTCCTA CAGCTACAAG 
CAGTCCTTGA ATATGACTGC TGTTGGCATC ACAGAGAATG TGAAGGGTGA CACCAAGAAG 
TTCGAGATCT GGTACAACGC AAGGGAGGAG GTTTACATCA TTCAGGCACC AACTCCCGAG 
ATTAAAGCAG CGTGGGTGAA TGAGATTCGG AAGGTGCTGA CCAGCCAACT GCAGGCCTGC 
CGGGAAGCCA GCCAGCACCG AGCCCTGGAG CAGTCCCACA GCCTTCCCTT GCCCACGCCA 
GCCAGCACCA GTCCCACAAA AGGGAGCACA AGAAATGTCA AGAAGCTCGA AGATCGGAAG 
ACGGACCCGC TCTGCCTGGA AGGCTGCGTG AGCTCATCAT TACCAAAGCC CCCCGAGAAG 
GGCAAAGACG ATGCGGTCCC TAGTTCTACC TCTGAAAGCT CTGCGCTTTC CAGAAAGCGC 
TTCACGCTGC AGGGCTTGGC TAACCTCAAA GGTCAGAAAG CCTCTCCGAC CAGTCCTGAC 
AAAAAAGCTA AGCGGCATGA AGTAAAGAGC GACCCGACTC CCTTTGGTTT GCGAGGCTGG 
AGCAAAACAT CCCACTCACT GGAGGCCCCT GAGGAGGACG GAGGCTGGTC CAGTGCTGAG 
GAGCTCATCA ATTCATCCGA TGCTGAGGAA GACGGAGGAG TGGGCCCTAG GAAGCTGGTT 
CCAGGTAAAT ACACAGTGCT GATGGATGGT GAGAAAGGGG GCTCTGACAC CCTTGCCATG 
AGGAGTGGAG ATATGGTGGA GGTTGTGGAA GAAGGAACTG AGGGCCTCTG GTATGTTCGG 
GACCTGACCA GCAGCAAGGA GGGATGGGTG CCAGCCAGCA GCCTAGCCAC CCTCCTGGGC 
AAATCCAGCT CAGCCCAGTG TCTGAGCAGC TCAGACTCCA GCCCAGGGTC AGCCGTGCTG 
AGCAACTCAT CCAGCTGCAG CGAGGGCTAC CCTACTCCCT TCTCCGAGCT CCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
Oncology reports28(2)429-38(2012 Aug)
B?hm D,Keller K,Pieter J,Boehm N,Wolters D,Siggelkow W,Lebrecht A,Schmidt M,K?lbl H,Pfeiffer N,Grus FH

Ccpg1, a novel scaffold protein that regulates the activity of the Rho guanine nucleotide exchange factor Dbs.
Molecular and cellular biology26(23)8964-75(2006 Dec)
Kostenko EV,Olabisi OO,Sahay S,Rodriguez PL,Whitehead IP

Role of the Sec14-like domain of Dbl family exchange factors in the regulation of Rho family GTPases in different subcellular sites.
Cellular signalling16(8)899-906(2004 Aug)
Ueda S,Kataoka T,Satoh T

Multifunctional roles for the PH domain of Dbs in regulating Rho GTPase activation.
The Journal of biological chemistry278(20)18393-400(2003 May)
Rossman KL,Cheng L,Mahon GM,Rojas RJ,Snyder JT,Whitehead IP,Sondek J

A novel oncogene, ost, encodes a guanine nucleotide exchange factor that potentially links Rho and Rac signaling pathways.
The EMBO journal13(20)4776-86(1994 Oct)
Horii Y,Beeler JF,Sakaguchi K,Tachibana M,Miki T

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Mcf2l cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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