mcf2l gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012957900.2	XP_012813354.1	guanine nucleotide exchange factor DBS isoform X1
XM_012957901.3	XP_012813355.1	guanine nucleotide exchange factor DBS isoform X1
XM_012957903.3	XP_012813357.1	guanine nucleotide exchange factor DBS isoform X3
XM_031897031.1	XP_031752891.1	guanine nucleotide exchange factor DBS isoform X5
XM_012957903.2	XP_012813357.1	guanine nucleotide exchange factor DBS isoform X2
XM_012957906.2	XP_012813360.1	guanine nucleotide exchange factor DBS isoform X5
XM_012957900.3	XP_012813354.1	guanine nucleotide exchange factor DBS isoform X1
XM_031897030.1	XP_031752890.1	guanine nucleotide exchange factor DBS isoform X2
XM_012957906.3	XP_012813360.1	guanine nucleotide exchange factor DBS isoform X8
XM_031897032.1	XP_031752892.1	guanine nucleotide exchange factor DBS isoform X7
XM_031897033.1	XP_031752893.1	guanine nucleotide exchange factor DBS isoform X7
XM_012957901.2	XP_012813355.1	guanine nucleotide exchange factor DBS isoform X1
XM_012957904.2	XP_012813358.1	guanine nucleotide exchange factor DBS isoform X3
XM_002933006.5	XP_002933052.2	guanine nucleotide exchange factor DBS isoform X6
XM_002933006.4	XP_002933052.2	guanine nucleotide exchange factor DBS isoform X4
XM_012957904.3	XP_012813358.1	guanine nucleotide exchange factor DBS isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa06192	XM_002933006.5 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa52505	XM_012957900.3 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52505	XM_012957901.3 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52506	XM_031897030.1 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52507	XM_012957903.3 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52508	XM_012957904.3 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52509	XM_031897031.1 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52510	XM_031897033.1 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa52510	XM_031897032.1 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa52511	XM_012957906.3 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa36977	XM_012957900.2 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa36977	XM_012957901.2 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa36979	XM_012957903.2 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa36980	XM_012957904.2 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa36982	XM_012957906.2 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OXa06192	XM_002933006.4	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

mcf2l ( XM_002933006.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933052.2 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa06192
Clone ID Related Accession (Same CDS sequence)	XM_002933006.5 , XM_002933006.4
Accession Version	XM_002933006.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933006.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGGTTGGAA CAGAGCATCA 
CATTCACTTG ATGTCTCTGA AGAAAATAAT GAAGATTGGT CTAGCGGAGA GGATCCAATT 
AACTCTTCAG ATGCAGAAGA AGATGGAGGA GGTGATGCAC CAGAGAAAAA GCTAACTCCT 
GGTAAATATA CTGTTGTGGC TGATTACGAG AGAGGTTCAG ATGAGCTGCT GGTAAAGAGT 
GGAGATCTTG TTCACATAAT ACATGAAGAG GATGGTGGAA TGTGGTATGT GAAGAACCTG 
AGTACCAATA AGGAAGGTCT GGTACCTGCT AGCAACCTAA TGACTCTCAT AGGCACTTCC 
AAATCTGCCC TCTCTTTAAG CAGCTCTGAA TCGGGACCCG CTTCAAGCAC TTTAAGCACA 
TCTTCTAGCT GTAGTGAGAG CTGCAATGCA GGCACCACCA GTTTCTCTGA TATCAAGGGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933052.2
CDS	513..3995
Translation	MSECWGLLICRALGSGNCFAAFRPCSEEEVDVNDHKVVVPDIIQLVPDDQSELELPSDDIMQQETFPICAADIEDQLRKQFAYLSGGRGENGCPVVTFPEYPAFSEIPEKEFQNVLTYLTSVPILQDAGIGFILVIDRRLDKWTSVKASILRIAASFPGNLQLVIVLRPSGLLQRTLSDIAFKFNKDDFKMKVPIIMLNSATELHSYIDKTQLTRDLGGTLDYCHTNWLSHRTAIENFAVMVKQTAQMLQSFGTELAETELPNDVPSTSLLLHAHTEKKEKMKDHLKIALTQGCDILNKIMEPRKENPEYRMNQDQLDNQTTVERLLLQLNETEAAFDEFWSKHEQKLEQCLQLRHFELNFREISTSLDGISERLEAFTDVGNSCSHAEHILKDFCNFEVKSREVLQTAQNLVREGDQLIKSNHYAVDSILPKCDEIGHLCDSISNTIQQRRQNLHKSLELHRHLEKTMKWCDEGIFLLASQPVDKFQSQDGAEAALQEITRFLETGTDITMKDLAFILKMYNSILTPELQEHVNKVYQKQESMQEMFEKRKGSLRKLAAKQTRPVQPVAPRPEAISKSPTSSPGLHRSSESRYSSGSIQRRINYRRAKSEVDGSQMRSTSVMEDEENLAVLRRHVMNELIETERAYVEELLSVIEGYAAEMNNPLVMDLIPSVLQNKKDVLFGNMEEIYQFHNRIFLRELEHYRDYPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDAAFFQDCQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALASILGILKAVNDSMHQIAITGYEGNLHELGKLLMQGSFNVWTEHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKRDENGEGYEKAPSYSYKHSLNMMFVGITEYVKGDNRKFEIWYNAREEVYIVQAPTTEVKATWVNEIRKVLTSQLKAHRDASHHKSAEHANSLPTPLPSSTSPLRNKSGKIKKLEERKTEPLLNPDVSLTSVPKYLEKGKGWNRASHSLDVSEENNEDWSSGEDPINSSDAEEDGGGDAPEKKLTPGKYTVVADYERGSDELLVKSGDLVHIIHEEDGGMWYVKNLSTNKEGLVPASNLMTLIGTSKSALSLSSSESGPASSTLSTSSSCSESCNAGTTSFSDIKG

Target ORF information:

RefSeq Version	XM_002933006.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933006.5

ORF Insert Sequence:

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGGTTGGAA CAGAGCATCA 
CATTCACTTG ATGTCTCTGA AGAAAATAAT GAAGATTGGT CTAGCGGAGA GGATCCAATT 
AACTCTTCAG ATGCAGAAGA AGATGGAGGA GGTGATGCAC CAGAGAAAAA GCTAACTCCT 
GGTAAATATA CTGTTGTGGC TGATTACGAG AGAGGTTCAG ATGAGCTGCT GGTAAAGAGT 
GGAGATCTTG TTCACATAAT ACATGAAGAG GATGGTGGAA TGTGGTATGT GAAGAACCTG 
AGTACCAATA AGGAAGGTCT GGTACCTGCT AGCAACCTAA TGACTCTCAT AGGCACTTCC 
AAATCTGCCC TCTCTTTAAG CAGCTCTGAA TCGGGACCCG CTTCAAGCAC TTTAAGCACA 
TCTTCTAGCT GTAGTGAGAG CTGCAATGCA GGCACCACCA GTTTCTCTGA TATCAAGGGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l ( XM_012957901.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813355.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa52505
Clone ID Related Accession (Same CDS sequence)	XM_012957901.3 , XM_012957900.3
Accession Version	XM_012957901.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957901.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG 
GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC 
TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT 
AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813355.1
CDS	36..3686
Translation	MSECWGLLICRALGSGNCFAAFRPCSEEEVDVNDHKVVVPDIIQLVPDDQSELELPSDDIMQQETFPICAADIEDQLRKQFAYLSGGRGENGCPVVTFPEYPAFSEIPEKEFQNVLTYLTSVPILQDAGIGFILVIDRRLDKWTSVKASILRIAASFPGNLQLVIVLRPSGLLQRTLSDIAFKFNKDDFKMKVPIIMLNSATELHSYIDKTQLTRDLGGTLDYCHTNWLSHRTAIENFAVMVKQTAQMLQSFGTELAETELPNDVPSTSLLLHAHTEKKEKMKDHLKIALTQGCDILNKIMEPRKENPEYRMNQDQLDNQTTVERLLLQLNETEAAFDEFWSKHEQKLEQCLQLRHFELNFREISTSLDGISERLEAFTDVGNSCSHAEHILKDFCNFEVKSREVLQTAQNLVREGDQLIKSNHYAVDSILPKCDEIGHLCDSISNTIQQRRQNLHKSLELHRHLEKTMKWCDEGIFLLASQPVDKFQSQDGAEAALQEITRFLETGTDITMKDLAFILKMYNSILTPELQEHVNKVYQKQESMQEMFEKRKGSLRKLAAKQTRPVQPVAPRPEAISKSPTSSPGLHRSSESRYSSGSIQRRINYRRAKSEVDGSQMRSTSVMEDEENLAVLRRHVMNELIETERAYVEELLSVIEGYAAEMNNPLVMDLIPSVLQNKKDVLFGNMEEIYQFHNRIFLRELEHYRDYPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDAAFFQDCQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALASILGILKAVNDSMHQIAITGYEGNLHELGKLLMQGSFNVWTEHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKRDENGEGYEKAPSYSYKHSLNMMFVGITEYVKGDNRKFEIWYNAREEVYIVQAPTTEVKATWVNEIRKVLTSQLKAHRDASHHKSAEHANSLPTPLPSSTSPLRNKSGKIKKLEERKTEPLLNPDVSLTSVPKYLEKGKDGTVTSPTSDSSALSKKRFTLQGFSNLKSQKASPTSPDKKAKRHEVKSDPTPFGLRGWNRASHSLDVSEENNEDWSSGEDPINSSDAEEDGGGDAPEKKLTPGKYTVVADYERGSDELLVKSGDLVHIIHEEDGGMWYVKNLSTNKEGLVPASNLMTLIGTSKSALSLSSSESGPASSTLSTSSSCSESCNAGTTSFSDIKG

Target ORF information:

RefSeq Version	XM_012957901.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957901.3

ORF Insert Sequence:

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG 
GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC 
TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT 
AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l ( XM_012957900.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813354.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X1, mRNA.

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CloneID	OXa52505
Clone ID Related Accession (Same CDS sequence)	XM_012957901.3 , XM_012957900.3
Accession Version	XM_012957900.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957900.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG 
GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC 
TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT 
AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813354.1
CDS	514..4164
Translation	MSECWGLLICRALGSGNCFAAFRPCSEEEVDVNDHKVVVPDIIQLVPDDQSELELPSDDIMQQETFPICAADIEDQLRKQFAYLSGGRGENGCPVVTFPEYPAFSEIPEKEFQNVLTYLTSVPILQDAGIGFILVIDRRLDKWTSVKASILRIAASFPGNLQLVIVLRPSGLLQRTLSDIAFKFNKDDFKMKVPIIMLNSATELHSYIDKTQLTRDLGGTLDYCHTNWLSHRTAIENFAVMVKQTAQMLQSFGTELAETELPNDVPSTSLLLHAHTEKKEKMKDHLKIALTQGCDILNKIMEPRKENPEYRMNQDQLDNQTTVERLLLQLNETEAAFDEFWSKHEQKLEQCLQLRHFELNFREISTSLDGISERLEAFTDVGNSCSHAEHILKDFCNFEVKSREVLQTAQNLVREGDQLIKSNHYAVDSILPKCDEIGHLCDSISNTIQQRRQNLHKSLELHRHLEKTMKWCDEGIFLLASQPVDKFQSQDGAEAALQEITRFLETGTDITMKDLAFILKMYNSILTPELQEHVNKVYQKQESMQEMFEKRKGSLRKLAAKQTRPVQPVAPRPEAISKSPTSSPGLHRSSESRYSSGSIQRRINYRRAKSEVDGSQMRSTSVMEDEENLAVLRRHVMNELIETERAYVEELLSVIEGYAAEMNNPLVMDLIPSVLQNKKDVLFGNMEEIYQFHNRIFLRELEHYRDYPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDAAFFQDCQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALASILGILKAVNDSMHQIAITGYEGNLHELGKLLMQGSFNVWTEHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKRDENGEGYEKAPSYSYKHSLNMMFVGITEYVKGDNRKFEIWYNAREEVYIVQAPTTEVKATWVNEIRKVLTSQLKAHRDASHHKSAEHANSLPTPLPSSTSPLRNKSGKIKKLEERKTEPLLNPDVSLTSVPKYLEKGKDGTVTSPTSDSSALSKKRFTLQGFSNLKSQKASPTSPDKKAKRHEVKSDPTPFGLRGWNRASHSLDVSEENNEDWSSGEDPINSSDAEEDGGGDAPEKKLTPGKYTVVADYERGSDELLVKSGDLVHIIHEEDGGMWYVKNLSTNKEGLVPASNLMTLIGTSKSALSLSSSESGPASSTLSTSSSCSESCNAGTTSFSDIKG

Target ORF information:

RefSeq Version	XM_012957900.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957900.3

ORF Insert Sequence:

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG 
GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC 
TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT 
AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l ( XM_031897030.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752890.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa52506
Clone ID Related Accession (Same CDS sequence)	XM_031897030.1
Accession Version	XM_031897030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC AAAGAAGAGA GAGGCACGAG GGCCAGCACA ATGGTAGGTT CTCCTCACGA 
GTGACTCATG TACTTCACAG TGCGTCAGCC TCGGTTCTCC GCTCCCTACC CAGGAGAGGA 
TGGAAGAAAA GCGCCCATGC CGCTTATTTT GATGACATCA TGCAACAGGA GACCTTTCCA 
ATCTGTGCAG CCGATATTGA GGATCAGCTA AGAAAGCAGT TTGCCTACTT GTCAGGTGGA 
AGAGGGGAAA ATGGCTGTCC AGTCGTCACA TTCCCTGAAT ACCCAGCATT TAGCGAGATT 
CCAGAAAAAG AGTTCCAGAA TGTACTCACC TATCTGACAA GTGTCCCAAT TTTACAAGAT 
GCGGGAATTG GCTTCATCTT AGTGATAGAC AGAAGGCTGG ACAAATGGAC CTCAGTGAAA 
GCCTCTATCC TGAGGATTGC GGCATCTTTT CCTGGGAATT TACAGCTTGT TATAGTCCTG 
CGCCCATCAG GACTATTGCA GCGCACCCTC TCGGATATTG CATTCAAATT CAACAAAGAT 
GACTTTAAAA TGAAAGTACC GATCATAATG CTGAATTCAG CAACAGAACT CCATAGTTAT 
ATTGACAAGA CCCAGCTGAC TCGAGATCTT GGTGGCACTC TGGATTACTG CCATACTAAC 
TGGCTCTCAC ATCGCACTGC TATTGAAAAC TTTGCTGTCA TGGTGAAACA AACAGCCCAA 
ATGCTCCAGT CTTTTGGGAC AGAGCTGGCA GAGACAGAGC TGCCAAATGA TGTTCCCTCT 
ACAAGCTTGC TGCTCCACGC ACACACAGAG AAAAAGGAAA AAATGAAGGA TCACCTTAAG 
ATAGCACTGA CACAGGGATG TGACATTTTG AACAAGATCA TGGAGCCACG TAAAGAGAAC 
CCTGAGTATA GGATGAATCA GGACCAACTG GATAACCAAA CTACTGTGGA AAGGCTTCTT 
TTGCAATTAA ATGAAACAGA AGCTGCTTTT GATGAGTTTT GGTCTAAACA TGAACAGAAG 
CTTGAACAGT GTTTACAGCT GCGTCATTTT GAGTTAAACT TCAGAGAGAT TTCTACAAGT 
CTGGATGGTA TTTCTGAGAG GCTAGAAGCT TTTACAGATG TTGGAAACAG CTGCTCCCAT 
GCTGAACACA TCCTGAAGGA CTTCTGTAAC TTTGAAGTTA AATCCCGAGA AGTTTTACAG 
ACAGCCCAGA ATTTAGTCAG AGAAGGTGAT CAACTCATTA AAAGTAATCA CTATGCTGTG 
GACTCCATTC TTCCAAAATG CGACGAGATT GGTCACTTAT GTGACAGCAT TAGCAACACT 
ATTCAGCAGA GACGGCAAAA CCTTCACAAA TCCTTGGAAC TTCACAGACA TTTGGAGAAG 
ACAATGAAGT GGTGTGATGA AGGGATTTTC TTGCTGGCGT CCCAACCTGT GGATAAGTTC 
CAGTCTCAGG ATGGGGCCGA AGCTGCACTT CAGGAGATTA CAAGGTTTCT AGAGACTGGA 
ACAGATATAA CCATGAAAGA TTTGGCTTTC ATTTTGAAGA TGTACAACTC GATTCTCACT 
CCAGAACTCC AGGAACATGT CAATAAGGTA TACCAGAAGC AGGAAAGCAT GCAGGAGATG 
TTTGAGAAAA GGAAAGGTAG CCTTAGAAAA CTTGCAGCAA AGCAAACCAG ACCTGTCCAG 
CCTGTTGCAC CTAGACCAGA AGCAATTTCC AAGTCTCCTA CCTCCTCTCC AGGTCTTCAT 
AGGTCTTCTG AAAGCAGATA TAGCTCAGGA AGTATTCAAC GACGGATAAA TTATCGCAGA 
GCCAAGAGCG AAGTGGATGG TAGTCAGATG AGAAGTACAT CAGTGATGGA AGACGAAGAA 
AATCTAGCAG TGTTAAGAAG ACATGTCATG AATGAACTTA TTGAGACAGA AAGGGCATAT 
GTGGAAGAAC TTCTATCTGT GATTGAGGGT TATGCTGCTG AAATGAATAA TCCTCTAGTG 
ATGGACCTTA TCCCCTCAGT GCTCCAGAAT AAGAAGGATG TTCTTTTCGG CAACATGGAA 
GAAATTTACC AGTTTCATAA CAGAATATTT CTGAGAGAGC TGGAACATTA TAGAGACTAT 
CCAGAACTAG TGGGAAGGTG TTTTTTGGAA AGGATGGAAG ATTTCCAGAT CTATGAAAAG 
TACTGCCAGA ACAAGCCACG GTCAGAGAGT TTGTGGAGGC AATGCTCCGA TGCTGCTTTC 
TTCCAGGATT GCCAAAAGAA ACTGGACCAT AAACTCAGCC TGGATTCCTA TCTTCTAAAG 
CCGGTGCAAA GGATAACAAA GTATCAGCTT CTGTTAAAGG AAATGCTTAA GTATAGTAAA 
AACTGTGAAG GAGCTGAAGA TCTTCAGGAA GCACTTGCCT CTATATTGGG CATTCTTAAA 
GCTGTCAATG ATTCCATGCA TCAGATAGCA ATCACTGGAT ATGAGGGGAA CTTACATGAA 
CTTGGTAAAC TCTTAATGCA AGGATCATTT AATGTATGGA CAGAGCACAA GAAGGGCCAT 
TCCAAAGTAA AGGACCTTGC TAGGTTTAAA CCAATGCAAA GACACCTGTT TCTGCATGAA 
AAAGCTGTGC TGTTCTGCAA GAAGAGGGAT GAGAATGGGG AGGGCTATGA GAAGGCACCA 
TCCTACAGCT ACAAACATTC CTTGAATATG ATGTTTGTTG GAATTACTGA GTATGTAAAA 
GGAGACAATA GGAAATTTGA AATTTGGTAC AACGCAAGGG AGGAGGTTTA TATTGTGCAG 
GCACCAACCA CAGAAGTAAA GGCCACCTGG GTAAATGAAA TCAGAAAGGT GCTGACAAGC 
CAGTTAAAGG CACACAGAGA TGCCAGCCAC CATAAATCGG CAGAGCACGC AAACAGCTTA 
CCTACTCCTC TCCCATCCAG TACTAGTCCC CTGAGAAACA AATCTGGGAA GATCAAAAAG 
CTGGAGGAAA GAAAAACTGA GCCTTTATTG AATCCGGATG TCTCCTTAAC ATCTGTACCA 
AAATACCTTG AAAAAGGCAA AGATGGCACA GTAACTAGTC CTACCTCAGA CTCTTCTGCC 
TTGTCAAAAA AGCGGTTCAC TTTGCAGGGT TTTTCAAACC TCAAAAGTCA GAAAGCCTCT 
CCTACCAGCC CAGACAAAAA AGCTAAACGC CATGAAGTAA AGAGTGACCC AACTCCCTTT 
GGTTTAAGAG GTTGGAACAG AGCATCACAT TCACTTGATG TCTCTGAAGA AAATAATGAA 
GATTGGTCTA GCGGAGAGGA TCCAATTAAC TCTTCAGATG CAGAAGAAGA TGGAGGAGGT 
GATGCACCAG AGAAAAAGCT AACTCCTGGT AAATATACTG TTGTGGCTGA TTACGAGAGA 
GGTTCAGATG AGCTGCTGGT AAAGAGTGGA GATCTTGTTC ACATAATACA TGAAGAGGAT 
GGTGGAATGT GGTATGTGAA GAACCTGAGT ACCAATAAGG AAGGTCTGGT ACCTGCTAGC 
AACCTAATGA CTCTCATAGG CACTTCCAAA TCTGCCCTCT CTTTAAGCAG CTCTGAATCG 
GGACCCGCTT CAAGCACTTT AAGCACATCT TCTAGCTGTA GTGAGAGCTG CAATGCAGGC 
ACCACCAGTT TCTCTGATAT CAAGGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752890.1
CDS	605..4234
Translation	MAQQRRERHEGQHNGRFSSRVTHVLHSASASVLRSLPRRGWKKSAHAAYFDDIMQQETFPICAADIEDQLRKQFAYLSGGRGENGCPVVTFPEYPAFSEIPEKEFQNVLTYLTSVPILQDAGIGFILVIDRRLDKWTSVKASILRIAASFPGNLQLVIVLRPSGLLQRTLSDIAFKFNKDDFKMKVPIIMLNSATELHSYIDKTQLTRDLGGTLDYCHTNWLSHRTAIENFAVMVKQTAQMLQSFGTELAETELPNDVPSTSLLLHAHTEKKEKMKDHLKIALTQGCDILNKIMEPRKENPEYRMNQDQLDNQTTVERLLLQLNETEAAFDEFWSKHEQKLEQCLQLRHFELNFREISTSLDGISERLEAFTDVGNSCSHAEHILKDFCNFEVKSREVLQTAQNLVREGDQLIKSNHYAVDSILPKCDEIGHLCDSISNTIQQRRQNLHKSLELHRHLEKTMKWCDEGIFLLASQPVDKFQSQDGAEAALQEITRFLETGTDITMKDLAFILKMYNSILTPELQEHVNKVYQKQESMQEMFEKRKGSLRKLAAKQTRPVQPVAPRPEAISKSPTSSPGLHRSSESRYSSGSIQRRINYRRAKSEVDGSQMRSTSVMEDEENLAVLRRHVMNELIETERAYVEELLSVIEGYAAEMNNPLVMDLIPSVLQNKKDVLFGNMEEIYQFHNRIFLRELEHYRDYPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDAAFFQDCQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALASILGILKAVNDSMHQIAITGYEGNLHELGKLLMQGSFNVWTEHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKRDENGEGYEKAPSYSYKHSLNMMFVGITEYVKGDNRKFEIWYNAREEVYIVQAPTTEVKATWVNEIRKVLTSQLKAHRDASHHKSAEHANSLPTPLPSSTSPLRNKSGKIKKLEERKTEPLLNPDVSLTSVPKYLEKGKDGTVTSPTSDSSALSKKRFTLQGFSNLKSQKASPTSPDKKAKRHEVKSDPTPFGLRGWNRASHSLDVSEENNEDWSSGEDPINSSDAEEDGGGDAPEKKLTPGKYTVVADYERGSDELLVKSGDLVHIIHEEDGGMWYVKNLSTNKEGLVPASNLMTLIGTSKSALSLSSSESGPASSTLSTSSSCSESCNAGTTSFSDIKG

Target ORF information:

RefSeq Version	XM_031897030.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897030.1

ORF Insert Sequence:

ATGGCTCAGC AAAGAAGAGA GAGGCACGAG GGCCAGCACA ATGGTAGGTT CTCCTCACGA 
GTGACTCATG TACTTCACAG TGCGTCAGCC TCGGTTCTCC GCTCCCTACC CAGGAGAGGA 
TGGAAGAAAA GCGCCCATGC CGCTTATTTT GATGACATCA TGCAACAGGA GACCTTTCCA 
ATCTGTGCAG CCGATATTGA GGATCAGCTA AGAAAGCAGT TTGCCTACTT GTCAGGTGGA 
AGAGGGGAAA ATGGCTGTCC AGTCGTCACA TTCCCTGAAT ACCCAGCATT TAGCGAGATT 
CCAGAAAAAG AGTTCCAGAA TGTACTCACC TATCTGACAA GTGTCCCAAT TTTACAAGAT 
GCGGGAATTG GCTTCATCTT AGTGATAGAC AGAAGGCTGG ACAAATGGAC CTCAGTGAAA 
GCCTCTATCC TGAGGATTGC GGCATCTTTT CCTGGGAATT TACAGCTTGT TATAGTCCTG 
CGCCCATCAG GACTATTGCA GCGCACCCTC TCGGATATTG CATTCAAATT CAACAAAGAT 
GACTTTAAAA TGAAAGTACC GATCATAATG CTGAATTCAG CAACAGAACT CCATAGTTAT 
ATTGACAAGA CCCAGCTGAC TCGAGATCTT GGTGGCACTC TGGATTACTG CCATACTAAC 
TGGCTCTCAC ATCGCACTGC TATTGAAAAC TTTGCTGTCA TGGTGAAACA AACAGCCCAA 
ATGCTCCAGT CTTTTGGGAC AGAGCTGGCA GAGACAGAGC TGCCAAATGA TGTTCCCTCT 
ACAAGCTTGC TGCTCCACGC ACACACAGAG AAAAAGGAAA AAATGAAGGA TCACCTTAAG 
ATAGCACTGA CACAGGGATG TGACATTTTG AACAAGATCA TGGAGCCACG TAAAGAGAAC 
CCTGAGTATA GGATGAATCA GGACCAACTG GATAACCAAA CTACTGTGGA AAGGCTTCTT 
TTGCAATTAA ATGAAACAGA AGCTGCTTTT GATGAGTTTT GGTCTAAACA TGAACAGAAG 
CTTGAACAGT GTTTACAGCT GCGTCATTTT GAGTTAAACT TCAGAGAGAT TTCTACAAGT 
CTGGATGGTA TTTCTGAGAG GCTAGAAGCT TTTACAGATG TTGGAAACAG CTGCTCCCAT 
GCTGAACACA TCCTGAAGGA CTTCTGTAAC TTTGAAGTTA AATCCCGAGA AGTTTTACAG 
ACAGCCCAGA ATTTAGTCAG AGAAGGTGAT CAACTCATTA AAAGTAATCA CTATGCTGTG 
GACTCCATTC TTCCAAAATG CGACGAGATT GGTCACTTAT GTGACAGCAT TAGCAACACT 
ATTCAGCAGA GACGGCAAAA CCTTCACAAA TCCTTGGAAC TTCACAGACA TTTGGAGAAG 
ACAATGAAGT GGTGTGATGA AGGGATTTTC TTGCTGGCGT CCCAACCTGT GGATAAGTTC 
CAGTCTCAGG ATGGGGCCGA AGCTGCACTT CAGGAGATTA CAAGGTTTCT AGAGACTGGA 
ACAGATATAA CCATGAAAGA TTTGGCTTTC ATTTTGAAGA TGTACAACTC GATTCTCACT 
CCAGAACTCC AGGAACATGT CAATAAGGTA TACCAGAAGC AGGAAAGCAT GCAGGAGATG 
TTTGAGAAAA GGAAAGGTAG CCTTAGAAAA CTTGCAGCAA AGCAAACCAG ACCTGTCCAG 
CCTGTTGCAC CTAGACCAGA AGCAATTTCC AAGTCTCCTA CCTCCTCTCC AGGTCTTCAT 
AGGTCTTCTG AAAGCAGATA TAGCTCAGGA AGTATTCAAC GACGGATAAA TTATCGCAGA 
GCCAAGAGCG AAGTGGATGG TAGTCAGATG AGAAGTACAT CAGTGATGGA AGACGAAGAA 
AATCTAGCAG TGTTAAGAAG ACATGTCATG AATGAACTTA TTGAGACAGA AAGGGCATAT 
GTGGAAGAAC TTCTATCTGT GATTGAGGGT TATGCTGCTG AAATGAATAA TCCTCTAGTG 
ATGGACCTTA TCCCCTCAGT GCTCCAGAAT AAGAAGGATG TTCTTTTCGG CAACATGGAA 
GAAATTTACC AGTTTCATAA CAGAATATTT CTGAGAGAGC TGGAACATTA TAGAGACTAT 
CCAGAACTAG TGGGAAGGTG TTTTTTGGAA AGGATGGAAG ATTTCCAGAT CTATGAAAAG 
TACTGCCAGA ACAAGCCACG GTCAGAGAGT TTGTGGAGGC AATGCTCCGA TGCTGCTTTC 
TTCCAGGATT GCCAAAAGAA ACTGGACCAT AAACTCAGCC TGGATTCCTA TCTTCTAAAG 
CCGGTGCAAA GGATAACAAA GTATCAGCTT CTGTTAAAGG AAATGCTTAA GTATAGTAAA 
AACTGTGAAG GAGCTGAAGA TCTTCAGGAA GCACTTGCCT CTATATTGGG CATTCTTAAA 
GCTGTCAATG ATTCCATGCA TCAGATAGCA ATCACTGGAT ATGAGGGGAA CTTACATGAA 
CTTGGTAAAC TCTTAATGCA AGGATCATTT AATGTATGGA CAGAGCACAA GAAGGGCCAT 
TCCAAAGTAA AGGACCTTGC TAGGTTTAAA CCAATGCAAA GACACCTGTT TCTGCATGAA 
AAAGCTGTGC TGTTCTGCAA GAAGAGGGAT GAGAATGGGG AGGGCTATGA GAAGGCACCA 
TCCTACAGCT ACAAACATTC CTTGAATATG ATGTTTGTTG GAATTACTGA GTATGTAAAA 
GGAGACAATA GGAAATTTGA AATTTGGTAC AACGCAAGGG AGGAGGTTTA TATTGTGCAG 
GCACCAACCA CAGAAGTAAA GGCCACCTGG GTAAATGAAA TCAGAAAGGT GCTGACAAGC 
CAGTTAAAGG CACACAGAGA TGCCAGCCAC CATAAATCGG CAGAGCACGC AAACAGCTTA 
CCTACTCCTC TCCCATCCAG TACTAGTCCC CTGAGAAACA AATCTGGGAA GATCAAAAAG 
CTGGAGGAAA GAAAAACTGA GCCTTTATTG AATCCGGATG TCTCCTTAAC ATCTGTACCA 
AAATACCTTG AAAAAGGCAA AGATGGCACA GTAACTAGTC CTACCTCAGA CTCTTCTGCC 
TTGTCAAAAA AGCGGTTCAC TTTGCAGGGT TTTTCAAACC TCAAAAGTCA GAAAGCCTCT 
CCTACCAGCC CAGACAAAAA AGCTAAACGC CATGAAGTAA AGAGTGACCC AACTCCCTTT 
GGTTTAAGAG GTTGGAACAG AGCATCACAT TCACTTGATG TCTCTGAAGA AAATAATGAA 
GATTGGTCTA GCGGAGAGGA TCCAATTAAC TCTTCAGATG CAGAAGAAGA TGGAGGAGGT 
GATGCACCAG AGAAAAAGCT AACTCCTGGT AAATATACTG TTGTGGCTGA TTACGAGAGA 
GGTTCAGATG AGCTGCTGGT AAAGAGTGGA GATCTTGTTC ACATAATACA TGAAGAGGAT 
GGTGGAATGT GGTATGTGAA GAACCTGAGT ACCAATAAGG AAGGTCTGGT ACCTGCTAGC 
AACCTAATGA CTCTCATAGG CACTTCCAAA TCTGCCCTCT CTTTAAGCAG CTCTGAATCG 
GGACCCGCTT CAAGCACTTT AAGCACATCT TCTAGCTGTA GTGAGAGCTG CAATGCAGGC 
ACCACCAGTT TCTCTGATAT CAAGGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l ( XM_012957903.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813357.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa52507
Clone ID Related Accession (Same CDS sequence)	XM_012957903.3
Accession Version	XM_012957903.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957903.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTG TAAAAATTTC AGTGGAAGAA ATGAAAGTCT ACCATTGGTT TTCTGAGATG 
TGCTTAATGC TCCATTCTTC GGTGCACAAT GACATCATGC AACAGGAGAC CTTTCCAATC 
TGTGCAGCCG ATATTGAGGA TCAGCTAAGA AAGCAGTTTG CCTACTTGTC AGGTGGAAGA 
GGGGAAAATG GCTGTCCAGT CGTCACATTC CCTGAATACC CAGCATTTAG CGAGATTCCA 
GAAAAAGAGT TCCAGAATGT ACTCACCTAT CTGACAAGTG TCCCAATTTT ACAAGATGCG 
GGAATTGGCT TCATCTTAGT GATAGACAGA AGGCTGGACA AATGGACCTC AGTGAAAGCC 
TCTATCCTGA GGATTGCGGC ATCTTTTCCT GGGAATTTAC AGCTTGTTAT AGTCCTGCGC 
CCATCAGGAC TATTGCAGCG CACCCTCTCG GATATTGCAT TCAAATTCAA CAAAGATGAC 
TTTAAAATGA AAGTACCGAT CATAATGCTG AATTCAGCAA CAGAACTCCA TAGTTATATT 
GACAAGACCC AGCTGACTCG AGATCTTGGT GGCACTCTGG ATTACTGCCA TACTAACTGG 
CTCTCACATC GCACTGCTAT TGAAAACTTT GCTGTCATGG TGAAACAAAC AGCCCAAATG 
CTCCAGTCTT TTGGGACAGA GCTGGCAGAG ACAGAGCTGC CAAATGATGT TCCCTCTACA 
AGCTTGCTGC TCCACGCACA CACAGAGAAA AAGGAAAAAA TGAAGGATCA CCTTAAGATA 
GCACTGACAC AGGGATGTGA CATTTTGAAC AAGATCATGG AGCCACGTAA AGAGAACCCT 
GAGTATAGGA TGAATCAGGA CCAACTGGAT AACCAAACTA CTGTGGAAAG GCTTCTTTTG 
CAATTAAATG AAACAGAAGC TGCTTTTGAT GAGTTTTGGT CTAAACATGA ACAGAAGCTT 
GAACAGTGTT TACAGCTGCG TCATTTTGAG TTAAACTTCA GAGAGATTTC TACAAGTCTG 
GATGGTATTT CTGAGAGGCT AGAAGCTTTT ACAGATGTTG GAAACAGCTG CTCCCATGCT 
GAACACATCC TGAAGGACTT CTGTAACTTT GAAGTTAAAT CCCGAGAAGT TTTACAGACA 
GCCCAGAATT TAGTCAGAGA AGGTGATCAA CTCATTAAAA GTAATCACTA TGCTGTGGAC 
TCCATTCTTC CAAAATGCGA CGAGATTGGT CACTTATGTG ACAGCATTAG CAACACTATT 
CAGCAGAGAC GGCAAAACCT TCACAAATCC TTGGAACTTC ACAGACATTT GGAGAAGACA 
ATGAAGTGGT GTGATGAAGG GATTTTCTTG CTGGCGTCCC AACCTGTGGA TAAGTTCCAG 
TCTCAGGATG GGGCCGAAGC TGCACTTCAG GAGATTACAA GGTTTCTAGA GACTGGAACA 
GATATAACCA TGAAAGATTT GGCTTTCATT TTGAAGATGT ACAACTCGAT TCTCACTCCA 
GAACTCCAGG AACATGTCAA TAAGGTATAC CAGAAGCAGG AAAGCATGCA GGAGATGTTT 
GAGAAAAGGA AAGGTAGCCT TAGAAAACTT GCAGCAAAGC AAACCAGACC TGTCCAGCCT 
GTTGCACCTA GACCAGAAGC AATTTCCAAG TCTCCTACCT CCTCTCCAGG TCTTCATAGG 
TCTTCTGAAA GCAGATATAG CTCAGGAAGT ATTCAACGAC GGATAAATTA TCGCAGAGCC 
AAGAGCGAAG TGGATGGTAG TCAGATGAGA AGTACATCAG TGATGGAAGA CGAAGAAAAT 
CTAGCAGTGT TAAGAAGACA TGTCATGAAT GAACTTATTG AGACAGAAAG GGCATATGTG 
GAAGAACTTC TATCTGTGAT TGAGGGTTAT GCTGCTGAAA TGAATAATCC TCTAGTGATG 
GACCTTATCC CCTCAGTGCT CCAGAATAAG AAGGATGTTC TTTTCGGCAA CATGGAAGAA 
ATTTACCAGT TTCATAACAG AATATTTCTG AGAGAGCTGG AACATTATAG AGACTATCCA 
GAACTAGTGG GAAGGTGTTT TTTGGAAAGG ATGGAAGATT TCCAGATCTA TGAAAAGTAC 
TGCCAGAACA AGCCACGGTC AGAGAGTTTG TGGAGGCAAT GCTCCGATGC TGCTTTCTTC 
CAGGATTGCC AAAAGAAACT GGACCATAAA CTCAGCCTGG ATTCCTATCT TCTAAAGCCG 
GTGCAAAGGA TAACAAAGTA TCAGCTTCTG TTAAAGGAAA TGCTTAAGTA TAGTAAAAAC 
TGTGAAGGAG CTGAAGATCT TCAGGAAGCA CTTGCCTCTA TATTGGGCAT TCTTAAAGCT 
GTCAATGATT CCATGCATCA GATAGCAATC ACTGGATATG AGGGGAACTT ACATGAACTT 
GGTAAACTCT TAATGCAAGG ATCATTTAAT GTATGGACAG AGCACAAGAA GGGCCATTCC 
AAAGTAAAGG ACCTTGCTAG GTTTAAACCA ATGCAAAGAC ACCTGTTTCT GCATGAAAAA 
GCTGTGCTGT TCTGCAAGAA GAGGGATGAG AATGGGGAGG GCTATGAGAA GGCACCATCC 
TACAGCTACA AACATTCCTT GAATATGATG TTTGTTGGAA TTACTGAGTA TGTAAAAGGA 
GACAATAGGA AATTTGAAAT TTGGTACAAC GCAAGGGAGG AGGTTTATAT TGTGCAGGCA 
CCAACCACAG AAGTAAAGGC CACCTGGGTA AATGAAATCA GAAAGGTGCT GACAAGCCAG 
TTAAAGGCAC ACAGAGATGC CAGCCACCAT AAATCGGCAG AGCACGCAAA CAGCTTACCT 
ACTCCTCTCC CATCCAGTAC TAGTCCCCTG AGAAACAAAT CTGGGAAGAT CAAAAAGCTG 
GAGGAAAGAA AAACTGAGCC TTTATTGAAT CCGGATGTCT CCTTAACATC TGTACCAAAA 
TACCTTGAAA AAGGCAAAGA TGGCACAGTA ACTAGTCCTA CCTCAGACTC TTCTGCCTTG 
TCAAAAAAGC GGTTCACTTT GCAGGGTTTT TCAAACCTCA AAAGTCAGAA AGCCTCTCCT 
ACCAGCCCAG ACAAAAAAGC TAAACGCCAT GAAGTAAAGA GTGACCCAAC TCCCTTTGGT 
TTAAGAGGTT GGAACAGAGC ATCACATTCA CTTGATGTCT CTGAAGAAAA TAATGAAGAT 
TGGTCTAGCG GAGAGGATCC AATTAACTCT TCAGATGCAG AAGAAGATGG AGGAGGTGAT 
GCACCAGAGA AAAAGCTAAC TCCTGGTAAA TATACTGTTG TGGCTGATTA CGAGAGAGGT 
TCAGATGAGC TGCTGGTAAA GAGTGGAGAT CTTGTTCACA TAATACATGA AGAGGATGGT 
GGAATGTGGT ATGTGAAGAA CCTGAGTACC AATAAGGAAG GTCTGGTACC TGCTAGCAAC 
CTAATGACTC TCATAGGCAC TTCCAAATCT GCCCTCTCTT TAAGCAGCTC TGAATCGGGA 
CCCGCTTCAA GCACTTTAAG CACATCTTCT AGCTGTAGTG AGAGCTGCAA TGCAGGCACC 
ACCAGTTTCT CTGATATCAA GGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813357.1
CDS	149..3715
Translation	MDCVKISVEEMKVYHWFSEMCLMLHSSVHNDIMQQETFPICAADIEDQLRKQFAYLSGGRGENGCPVVTFPEYPAFSEIPEKEFQNVLTYLTSVPILQDAGIGFILVIDRRLDKWTSVKASILRIAASFPGNLQLVIVLRPSGLLQRTLSDIAFKFNKDDFKMKVPIIMLNSATELHSYIDKTQLTRDLGGTLDYCHTNWLSHRTAIENFAVMVKQTAQMLQSFGTELAETELPNDVPSTSLLLHAHTEKKEKMKDHLKIALTQGCDILNKIMEPRKENPEYRMNQDQLDNQTTVERLLLQLNETEAAFDEFWSKHEQKLEQCLQLRHFELNFREISTSLDGISERLEAFTDVGNSCSHAEHILKDFCNFEVKSREVLQTAQNLVREGDQLIKSNHYAVDSILPKCDEIGHLCDSISNTIQQRRQNLHKSLELHRHLEKTMKWCDEGIFLLASQPVDKFQSQDGAEAALQEITRFLETGTDITMKDLAFILKMYNSILTPELQEHVNKVYQKQESMQEMFEKRKGSLRKLAAKQTRPVQPVAPRPEAISKSPTSSPGLHRSSESRYSSGSIQRRINYRRAKSEVDGSQMRSTSVMEDEENLAVLRRHVMNELIETERAYVEELLSVIEGYAAEMNNPLVMDLIPSVLQNKKDVLFGNMEEIYQFHNRIFLRELEHYRDYPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDAAFFQDCQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALASILGILKAVNDSMHQIAITGYEGNLHELGKLLMQGSFNVWTEHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKRDENGEGYEKAPSYSYKHSLNMMFVGITEYVKGDNRKFEIWYNAREEVYIVQAPTTEVKATWVNEIRKVLTSQLKAHRDASHHKSAEHANSLPTPLPSSTSPLRNKSGKIKKLEERKTEPLLNPDVSLTSVPKYLEKGKDGTVTSPTSDSSALSKKRFTLQGFSNLKSQKASPTSPDKKAKRHEVKSDPTPFGLRGWNRASHSLDVSEENNEDWSSGEDPINSSDAEEDGGGDAPEKKLTPGKYTVVADYERGSDELLVKSGDLVHIIHEEDGGMWYVKNLSTNKEGLVPASNLMTLIGTSKSALSLSSSESGPASSTLSTSSSCSESCNAGTTSFSDIKG

Target ORF information:

RefSeq Version	XM_012957903.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957903.3

ORF Insert Sequence:

ATGGATTGTG TAAAAATTTC AGTGGAAGAA ATGAAAGTCT ACCATTGGTT TTCTGAGATG 
TGCTTAATGC TCCATTCTTC GGTGCACAAT GACATCATGC AACAGGAGAC CTTTCCAATC 
TGTGCAGCCG ATATTGAGGA TCAGCTAAGA AAGCAGTTTG CCTACTTGTC AGGTGGAAGA 
GGGGAAAATG GCTGTCCAGT CGTCACATTC CCTGAATACC CAGCATTTAG CGAGATTCCA 
GAAAAAGAGT TCCAGAATGT ACTCACCTAT CTGACAAGTG TCCCAATTTT ACAAGATGCG 
GGAATTGGCT TCATCTTAGT GATAGACAGA AGGCTGGACA AATGGACCTC AGTGAAAGCC 
TCTATCCTGA GGATTGCGGC ATCTTTTCCT GGGAATTTAC AGCTTGTTAT AGTCCTGCGC 
CCATCAGGAC TATTGCAGCG CACCCTCTCG GATATTGCAT TCAAATTCAA CAAAGATGAC 
TTTAAAATGA AAGTACCGAT CATAATGCTG AATTCAGCAA CAGAACTCCA TAGTTATATT 
GACAAGACCC AGCTGACTCG AGATCTTGGT GGCACTCTGG ATTACTGCCA TACTAACTGG 
CTCTCACATC GCACTGCTAT TGAAAACTTT GCTGTCATGG TGAAACAAAC AGCCCAAATG 
CTCCAGTCTT TTGGGACAGA GCTGGCAGAG ACAGAGCTGC CAAATGATGT TCCCTCTACA 
AGCTTGCTGC TCCACGCACA CACAGAGAAA AAGGAAAAAA TGAAGGATCA CCTTAAGATA 
GCACTGACAC AGGGATGTGA CATTTTGAAC AAGATCATGG AGCCACGTAA AGAGAACCCT 
GAGTATAGGA TGAATCAGGA CCAACTGGAT AACCAAACTA CTGTGGAAAG GCTTCTTTTG 
CAATTAAATG AAACAGAAGC TGCTTTTGAT GAGTTTTGGT CTAAACATGA ACAGAAGCTT 
GAACAGTGTT TACAGCTGCG TCATTTTGAG TTAAACTTCA GAGAGATTTC TACAAGTCTG 
GATGGTATTT CTGAGAGGCT AGAAGCTTTT ACAGATGTTG GAAACAGCTG CTCCCATGCT 
GAACACATCC TGAAGGACTT CTGTAACTTT GAAGTTAAAT CCCGAGAAGT TTTACAGACA 
GCCCAGAATT TAGTCAGAGA AGGTGATCAA CTCATTAAAA GTAATCACTA TGCTGTGGAC 
TCCATTCTTC CAAAATGCGA CGAGATTGGT CACTTATGTG ACAGCATTAG CAACACTATT 
CAGCAGAGAC GGCAAAACCT TCACAAATCC TTGGAACTTC ACAGACATTT GGAGAAGACA 
ATGAAGTGGT GTGATGAAGG GATTTTCTTG CTGGCGTCCC AACCTGTGGA TAAGTTCCAG 
TCTCAGGATG GGGCCGAAGC TGCACTTCAG GAGATTACAA GGTTTCTAGA GACTGGAACA 
GATATAACCA TGAAAGATTT GGCTTTCATT TTGAAGATGT ACAACTCGAT TCTCACTCCA 
GAACTCCAGG AACATGTCAA TAAGGTATAC CAGAAGCAGG AAAGCATGCA GGAGATGTTT 
GAGAAAAGGA AAGGTAGCCT TAGAAAACTT GCAGCAAAGC AAACCAGACC TGTCCAGCCT 
GTTGCACCTA GACCAGAAGC AATTTCCAAG TCTCCTACCT CCTCTCCAGG TCTTCATAGG 
TCTTCTGAAA GCAGATATAG CTCAGGAAGT ATTCAACGAC GGATAAATTA TCGCAGAGCC 
AAGAGCGAAG TGGATGGTAG TCAGATGAGA AGTACATCAG TGATGGAAGA CGAAGAAAAT 
CTAGCAGTGT TAAGAAGACA TGTCATGAAT GAACTTATTG AGACAGAAAG GGCATATGTG 
GAAGAACTTC TATCTGTGAT TGAGGGTTAT GCTGCTGAAA TGAATAATCC TCTAGTGATG 
GACCTTATCC CCTCAGTGCT CCAGAATAAG AAGGATGTTC TTTTCGGCAA CATGGAAGAA 
ATTTACCAGT TTCATAACAG AATATTTCTG AGAGAGCTGG AACATTATAG AGACTATCCA 
GAACTAGTGG GAAGGTGTTT TTTGGAAAGG ATGGAAGATT TCCAGATCTA TGAAAAGTAC 
TGCCAGAACA AGCCACGGTC AGAGAGTTTG TGGAGGCAAT GCTCCGATGC TGCTTTCTTC 
CAGGATTGCC AAAAGAAACT GGACCATAAA CTCAGCCTGG ATTCCTATCT TCTAAAGCCG 
GTGCAAAGGA TAACAAAGTA TCAGCTTCTG TTAAAGGAAA TGCTTAAGTA TAGTAAAAAC 
TGTGAAGGAG CTGAAGATCT TCAGGAAGCA CTTGCCTCTA TATTGGGCAT TCTTAAAGCT 
GTCAATGATT CCATGCATCA GATAGCAATC ACTGGATATG AGGGGAACTT ACATGAACTT 
GGTAAACTCT TAATGCAAGG ATCATTTAAT GTATGGACAG AGCACAAGAA GGGCCATTCC 
AAAGTAAAGG ACCTTGCTAG GTTTAAACCA ATGCAAAGAC ACCTGTTTCT GCATGAAAAA 
GCTGTGCTGT TCTGCAAGAA GAGGGATGAG AATGGGGAGG GCTATGAGAA GGCACCATCC 
TACAGCTACA AACATTCCTT GAATATGATG TTTGTTGGAA TTACTGAGTA TGTAAAAGGA 
GACAATAGGA AATTTGAAAT TTGGTACAAC GCAAGGGAGG AGGTTTATAT TGTGCAGGCA 
CCAACCACAG AAGTAAAGGC CACCTGGGTA AATGAAATCA GAAAGGTGCT GACAAGCCAG 
TTAAAGGCAC ACAGAGATGC CAGCCACCAT AAATCGGCAG AGCACGCAAA CAGCTTACCT 
ACTCCTCTCC CATCCAGTAC TAGTCCCCTG AGAAACAAAT CTGGGAAGAT CAAAAAGCTG 
GAGGAAAGAA AAACTGAGCC TTTATTGAAT CCGGATGTCT CCTTAACATC TGTACCAAAA 
TACCTTGAAA AAGGCAAAGA TGGCACAGTA ACTAGTCCTA CCTCAGACTC TTCTGCCTTG 
TCAAAAAAGC GGTTCACTTT GCAGGGTTTT TCAAACCTCA AAAGTCAGAA AGCCTCTCCT 
ACCAGCCCAG ACAAAAAAGC TAAACGCCAT GAAGTAAAGA GTGACCCAAC TCCCTTTGGT 
TTAAGAGGTT GGAACAGAGC ATCACATTCA CTTGATGTCT CTGAAGAAAA TAATGAAGAT 
TGGTCTAGCG GAGAGGATCC AATTAACTCT TCAGATGCAG AAGAAGATGG AGGAGGTGAT 
GCACCAGAGA AAAAGCTAAC TCCTGGTAAA TATACTGTTG TGGCTGATTA CGAGAGAGGT 
TCAGATGAGC TGCTGGTAAA GAGTGGAGAT CTTGTTCACA TAATACATGA AGAGGATGGT 
GGAATGTGGT ATGTGAAGAA CCTGAGTACC AATAAGGAAG GTCTGGTACC TGCTAGCAAC 
CTAATGACTC TCATAGGCAC TTCCAAATCT GCCCTCTCTT TAAGCAGCTC TGAATCGGGA 
CCCGCTTCAA GCACTTTAAG CACATCTTCT AGCTGTAGTG AGAGCTGCAA TGCAGGCACC 
ACCAGTTTCT CTGATATCAA GGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l ( XM_012957904.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813358.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X5, mRNA.

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CloneID	OXa52508
Clone ID Related Accession (Same CDS sequence)	XM_012957904.3
Accession Version	XM_012957904.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957904.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGCCTCTCC TACCAGCCCA 
GACAAAAAAG CTAAACGCCA TGAAGTAAAG AGTGACCCAA CTCCCTTTGG TTTAAGAGGT 
TGGAACAGAG CATCACATTC ACTTGATGTC TCTGAAGAAA ATAATGAAGA TTGGTCTAGC 
GGAGAGGATC CAATTAACTC TTCAGATGCA GAAGAAGATG GAGGAGGTGA TGCACCAGAG 
AAAAAGCTAA CTCCTGGTAA ATATACTGTT GTGGCTGATT ACGAGAGAGG TTCAGATGAG 
CTGCTGGTAA AGAGTGGAGA TCTTGTTCAC ATAATACATG AAGAGGATGG TGGAATGTGG 
TATGTGAAGA ACCTGAGTAC CAATAAGGAA GGTCTGGTAC CTGCTAGCAA CCTAATGACT 
CTCATAGGCA CTTCCAAATC TGCCCTCTCT TTAAGCAGCT CTGAATCGGG ACCCGCTTCA 
AGCACTTTAA GCACATCTTC TAGCTGTAGT GAGAGCTGCA ATGCAGGCAC CACCAGTTTC 
TCTGATATCA AGGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813358.1
CDS	513..4070
Translation	MSECWGLLICRALGSGNCFAAFRPCSEEEVDVNDHKVVVPDIIQLVPDDQSELELPSDDIMQQETFPICAADIEDQLRKQFAYLSGGRGENGCPVVTFPEYPAFSEIPEKEFQNVLTYLTSVPILQDAGIGFILVIDRRLDKWTSVKASILRIAASFPGNLQLVIVLRPSGLLQRTLSDIAFKFNKDDFKMKVPIIMLNSATELHSYIDKTQLTRDLGGTLDYCHTNWLSHRTAIENFAVMVKQTAQMLQSFGTELAETELPNDVPSTSLLLHAHTEKKEKMKDHLKIALTQGCDILNKIMEPRKENPEYRMNQDQLDNQTTVERLLLQLNETEAAFDEFWSKHEQKLEQCLQLRHFELNFREISTSLDGISERLEAFTDVGNSCSHAEHILKDFCNFEVKSREVLQTAQNLVREGDQLIKSNHYAVDSILPKCDEIGHLCDSISNTIQQRRQNLHKSLELHRHLEKTMKWCDEGIFLLASQPVDKFQSQDGAEAALQEITRFLETGTDITMKDLAFILKMYNSILTPELQEHVNKVYQKQESMQEMFEKRKGSLRKLAAKQTRPVQPVAPRPEAISKSPTSSPGLHRSSESRYSSGSIQRRINYRRAKSEVDGSQMRSTSVMEDEENLAVLRRHVMNELIETERAYVEELLSVIEGYAAEMNNPLVMDLIPSVLQNKKDVLFGNMEEIYQFHNRIFLRELEHYRDYPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDAAFFQDCQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALASILGILKAVNDSMHQIAITGYEGNLHELGKLLMQGSFNVWTEHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKRDENGEGYEKAPSYSYKHSLNMMFVGITEYVKGDNRKFEIWYNAREEVYIVQAPTTEVKATWVNEIRKVLTSQLKAHRDASHHKSAEHANSLPTPLPSSTSPLRNKSGKIKKLEERKTEPLLNPDVSLTSVPKYLEKGKASPTSPDKKAKRHEVKSDPTPFGLRGWNRASHSLDVSEENNEDWSSGEDPINSSDAEEDGGGDAPEKKLTPGKYTVVADYERGSDELLVKSGDLVHIIHEEDGGMWYVKNLSTNKEGLVPASNLMTLIGTSKSALSLSSSESGPASSTLSTSSSCSESCNAGTTSFSDIKG

Target ORF information:

RefSeq Version	XM_012957904.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957904.3

ORF Insert Sequence:

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGCCTCTCC TACCAGCCCA 
GACAAAAAAG CTAAACGCCA TGAAGTAAAG AGTGACCCAA CTCCCTTTGG TTTAAGAGGT 
TGGAACAGAG CATCACATTC ACTTGATGTC TCTGAAGAAA ATAATGAAGA TTGGTCTAGC 
GGAGAGGATC CAATTAACTC TTCAGATGCA GAAGAAGATG GAGGAGGTGA TGCACCAGAG 
AAAAAGCTAA CTCCTGGTAA ATATACTGTT GTGGCTGATT ACGAGAGAGG TTCAGATGAG 
CTGCTGGTAA AGAGTGGAGA TCTTGTTCAC ATAATACATG AAGAGGATGG TGGAATGTGG 
TATGTGAAGA ACCTGAGTAC CAATAAGGAA GGTCTGGTAC CTGCTAGCAA CCTAATGACT 
CTCATAGGCA CTTCCAAATC TGCCCTCTCT TTAAGCAGCT CTGAATCGGG ACCCGCTTCA 
AGCACTTTAA GCACATCTTC TAGCTGTAGT GAGAGCTGCA ATGCAGGCAC CACCAGTTTC 
TCTGATATCA AGGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l ( XM_031897031.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752891.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X6, mRNA.

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CloneID	OXa52509
Clone ID Related Accession (Same CDS sequence)	XM_031897031.1
Accession Version	XM_031897031.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGG AAGAACTGCT ACGGAGATTA AATGAGGTTT TACACAACAC TGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG 
GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC 
TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT 
AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752891.1
CDS	49..3579
Translation	MAVEELLRRLNEVLHNTDDIMQQETFPICAADIEDQLRKQFAYLSGGRGENGCPVVTFPEYPAFSEIPEKEFQNVLTYLTSVPILQDAGIGFILVIDRRLDKWTSVKASILRIAASFPGNLQLVIVLRPSGLLQRTLSDIAFKFNKDDFKMKVPIIMLNSATELHSYIDKTQLTRDLGGTLDYCHTNWLSHRTAIENFAVMVKQTAQMLQSFGTELAETELPNDVPSTSLLLHAHTEKKEKMKDHLKIALTQGCDILNKIMEPRKENPEYRMNQDQLDNQTTVERLLLQLNETEAAFDEFWSKHEQKLEQCLQLRHFELNFREISTSLDGISERLEAFTDVGNSCSHAEHILKDFCNFEVKSREVLQTAQNLVREGDQLIKSNHYAVDSILPKCDEIGHLCDSISNTIQQRRQNLHKSLELHRHLEKTMKWCDEGIFLLASQPVDKFQSQDGAEAALQEITRFLETGTDITMKDLAFILKMYNSILTPELQEHVNKVYQKQESMQEMFEKRKGSLRKLAAKQTRPVQPVAPRPEAISKSPTSSPGLHRSSESRYSSGSIQRRINYRRAKSEVDGSQMRSTSVMEDEENLAVLRRHVMNELIETERAYVEELLSVIEGYAAEMNNPLVMDLIPSVLQNKKDVLFGNMEEIYQFHNRIFLRELEHYRDYPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDAAFFQDCQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALASILGILKAVNDSMHQIAITGYEGNLHELGKLLMQGSFNVWTEHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKRDENGEGYEKAPSYSYKHSLNMMFVGITEYVKGDNRKFEIWYNAREEVYIVQAPTTEVKATWVNEIRKVLTSQLKAHRDASHHKSAEHANSLPTPLPSSTSPLRNKSGKIKKLEERKTEPLLNPDVSLTSVPKYLEKGKDGTVTSPTSDSSALSKKRFTLQGFSNLKSQKASPTSPDKKAKRHEVKSDPTPFGLRGWNRASHSLDVSEENNEDWSSGEDPINSSDAEEDGGGDAPEKKLTPGKYTVVADYERGSDELLVKSGDLVHIIHEEDGGMWYVKNLSTNKEGLVPASNLMTLIGTSKSALSLSSSESGPASSTLSTSSSCSESCNAGTTSFSDIKG

Target ORF information:

RefSeq Version	XM_031897031.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897031.1

ORF Insert Sequence:

ATGGCTGTGG AAGAACTGCT ACGGAGATTA AATGAGGTTT TACACAACAC TGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG 
GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC 
TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT 
AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l ( XM_031897032.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752892.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X8, mRNA.

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CloneID	OXa52510
Clone ID Related Accession (Same CDS sequence)	XM_031897032.1 , XM_031897033.1
Accession Version	XM_031897032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG 
GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC 
TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT 
AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752892.1
CDS	310..3780
Translation	MQQETFPICAADIEDQLRKQFAYLSGGRGENGCPVVTFPEYPAFSEIPEKEFQNVLTYLTSVPILQDAGIGFILVIDRRLDKWTSVKASILRIAASFPGNLQLVIVLRPSGLLQRTLSDIAFKFNKDDFKMKVPIIMLNSATELHSYIDKTQLTRDLGGTLDYCHTNWLSHRTAIENFAVMVKQTAQMLQSFGTELAETELPNDVPSTSLLLHAHTEKKEKMKDHLKIALTQGCDILNKIMEPRKENPEYRMNQDQLDNQTTVERLLLQLNETEAAFDEFWSKHEQKLEQCLQLRHFELNFREISTSLDGISERLEAFTDVGNSCSHAEHILKDFCNFEVKSREVLQTAQNLVREGDQLIKSNHYAVDSILPKCDEIGHLCDSISNTIQQRRQNLHKSLELHRHLEKTMKWCDEGIFLLASQPVDKFQSQDGAEAALQEITRFLETGTDITMKDLAFILKMYNSILTPELQEHVNKVYQKQESMQEMFEKRKGSLRKLAAKQTRPVQPVAPRPEAISKSPTSSPGLHRSSESRYSSGSIQRRINYRRAKSEVDGSQMRSTSVMEDEENLAVLRRHVMNELIETERAYVEELLSVIEGYAAEMNNPLVMDLIPSVLQNKKDVLFGNMEEIYQFHNRIFLRELEHYRDYPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDAAFFQDCQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALASILGILKAVNDSMHQIAITGYEGNLHELGKLLMQGSFNVWTEHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKRDENGEGYEKAPSYSYKHSLNMMFVGITEYVKGDNRKFEIWYNAREEVYIVQAPTTEVKATWVNEIRKVLTSQLKAHRDASHHKSAEHANSLPTPLPSSTSPLRNKSGKIKKLEERKTEPLLNPDVSLTSVPKYLEKGKDGTVTSPTSDSSALSKKRFTLQGFSNLKSQKASPTSPDKKAKRHEVKSDPTPFGLRGWNRASHSLDVSEENNEDWSSGEDPINSSDAEEDGGGDAPEKKLTPGKYTVVADYERGSDELLVKSGDLVHIIHEEDGGMWYVKNLSTNKEGLVPASNLMTLIGTSKSALSLSSSESGPASSTLSTSSSCSESCNAGTTSFSDIKG

Target ORF information:

RefSeq Version	XM_031897032.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897032.1

ORF Insert Sequence:

ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG 
GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC 
TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT 
AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l ( XM_031897033.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752893.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OXa52510
Clone ID Related Accession (Same CDS sequence)	XM_031897032.1 , XM_031897033.1
Accession Version	XM_031897033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG 
GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC 
TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT 
AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752893.1
CDS	225..3695
Translation	MQQETFPICAADIEDQLRKQFAYLSGGRGENGCPVVTFPEYPAFSEIPEKEFQNVLTYLTSVPILQDAGIGFILVIDRRLDKWTSVKASILRIAASFPGNLQLVIVLRPSGLLQRTLSDIAFKFNKDDFKMKVPIIMLNSATELHSYIDKTQLTRDLGGTLDYCHTNWLSHRTAIENFAVMVKQTAQMLQSFGTELAETELPNDVPSTSLLLHAHTEKKEKMKDHLKIALTQGCDILNKIMEPRKENPEYRMNQDQLDNQTTVERLLLQLNETEAAFDEFWSKHEQKLEQCLQLRHFELNFREISTSLDGISERLEAFTDVGNSCSHAEHILKDFCNFEVKSREVLQTAQNLVREGDQLIKSNHYAVDSILPKCDEIGHLCDSISNTIQQRRQNLHKSLELHRHLEKTMKWCDEGIFLLASQPVDKFQSQDGAEAALQEITRFLETGTDITMKDLAFILKMYNSILTPELQEHVNKVYQKQESMQEMFEKRKGSLRKLAAKQTRPVQPVAPRPEAISKSPTSSPGLHRSSESRYSSGSIQRRINYRRAKSEVDGSQMRSTSVMEDEENLAVLRRHVMNELIETERAYVEELLSVIEGYAAEMNNPLVMDLIPSVLQNKKDVLFGNMEEIYQFHNRIFLRELEHYRDYPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDAAFFQDCQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALASILGILKAVNDSMHQIAITGYEGNLHELGKLLMQGSFNVWTEHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKRDENGEGYEKAPSYSYKHSLNMMFVGITEYVKGDNRKFEIWYNAREEVYIVQAPTTEVKATWVNEIRKVLTSQLKAHRDASHHKSAEHANSLPTPLPSSTSPLRNKSGKIKKLEERKTEPLLNPDVSLTSVPKYLEKGKDGTVTSPTSDSSALSKKRFTLQGFSNLKSQKASPTSPDKKAKRHEVKSDPTPFGLRGWNRASHSLDVSEENNEDWSSGEDPINSSDAEEDGGGDAPEKKLTPGKYTVVADYERGSDELLVKSGDLVHIIHEEDGGMWYVKNLSTNKEGLVPASNLMTLIGTSKSALSLSSSESGPASSTLSTSSSCSESCNAGTTSFSDIKG

Target ORF information:

RefSeq Version	XM_031897033.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897033.1

ORF Insert Sequence:

ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG 
GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC 
TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT 
AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l ( XM_012957906.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813360.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OXa52511
Clone ID Related Accession (Same CDS sequence)	XM_012957906.3
Accession Version	XM_012957906.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957906.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813360.1
CDS	514..3966
Translation	MSECWGLLICRALGSGNCFAAFRPCSEEEVDVNDHKVVVPDIIQLVPDDQSELELPSDDIMQQETFPICAADIEDQLRKQFAYLSGGRGENGCPVVTFPEYPAFSEIPEKEFQNVLTYLTSVPILQDAGIGFILVIDRRLDKWTSVKASILRIAASFPGNLQLVIVLRPSGLLQRTLSDIAFKFNKDDFKMKVPIIMLNSATELHSYIDKTQLTRDLGGTLDYCHTNWLSHRTAIENFAVMVKQTAQMLQSFGTELAETELPNDVPSTSLLLHAHTEKKEKMKDHLKIALTQGCDILNKIMEPRKENPEYRMNQDQLDNQTTVERLLLQLNETEAAFDEFWSKHEQKLEQCLQLRHFELNFREISTSLDGISERLEAFTDVGNSCSHAEHILKDFCNFEVKSREVLQTAQNLVREGDQLIKSNHYAVDSILPKCDEIGHLCDSISNTIQQRRQNLHKSLELHRHLEKTMKWCDEGIFLLASQPVDKFQSQDGAEAALQEITRFLETGTDITMKDLAFILKMYNSILTPELQEHVNKVYQKQESMQEMFEKRKGSLRKLAAKQTRPVQPVAPRPEAISKSPTSSPGLHRSSESRYSSGSIQRRINYRRAKSEVDGSQMRSTSVMEDEENLAVLRRHVMNELIETERAYVEELLSVIEGYAAEMNNPLVMDLIPSVLQNKKDVLFGNMEEIYQFHNRIFLRELEHYRDYPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDAAFFQDCQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALASILGILKAVNDSMHQIAITGYEGNLHELGKLLMQGSFNVWTEHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKRDENGEGYEKAPSYSYKHSLNMMFVGITEYVKGDNRKFEIWYNAREEVYIVQAPTTEVKATWVNEIRKVLTSQLKAHRDASHHKSAEHANSLPTPLPSSTSPLRNKSGKIKKLEERKTEPLLNPDVSLTSVPKYLEKGKDGTVTSPTSDSSALSKKRFTLQGFSNLKSQKASPTSPDKKAKRHEVKSDPTPFGLRGWNRASHSLDVSEENNEDWSSGEDPINSSDAEEDGGGDAPEKKLTPGKYTVVADYERGSDELLVKSGDLVHIIHEEDGGM

Target ORF information:

RefSeq Version	XM_012957906.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957906.3

ORF Insert Sequence:

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l ( XM_002933006.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933052.2 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X5, mRNA.

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CloneID	OXa06192
Clone ID Related Accession (Same CDS sequence)	XM_002933006.5 , XM_002933006.4
Accession Version	XM_002933006.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933006.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGGTTGGAA CAGAGCATCA 
CATTCACTTG ATGTCTCTGA AGAAAATAAT GAAGATTGGT CTAGCGGAGA GGATCCAATT 
AACTCTTCAG ATGCAGAAGA AGATGGAGGA GGTGATGCAC CAGAGAAAAA GCTAACTCCT 
GGTAAATATA CTGTTGTGGC TGATTACGAG AGAGGTTCAG ATGAGCTGCT GGTAAAGAGT 
GGAGATCTTG TTCACATAAT ACATGAAGAG GATGGTGGAA TGTGGTATGT GAAGAACCTG 
AGTACCAATA AGGAAGGTCT GGTACCTGCT AGCAACCTAA TGACTCTCAT AGGCACTTCC 
AAATCTGCCC TCTCTTTAAG CAGCTCTGAA TCGGGACCCG CTTCAAGCAC TTTAAGCACA 
TCTTCTAGCT GTAGTGAGAG CTGCAATGCA GGCACCACCA GTTTCTCTGA TATCAAGGGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933052.2
CDS	672..4154
Translation	MSECWGLLICRALGSGNCFAAFRPCSEEEVDVNDHKVVVPDIIQLVPDDQSELELPSDDIMQQETFPICAADIEDQLRKQFAYLSGGRGENGCPVVTFPEYPAFSEIPEKEFQNVLTYLTSVPILQDAGIGFILVIDRRLDKWTSVKASILRIAASFPGNLQLVIVLRPSGLLQRTLSDIAFKFNKDDFKMKVPIIMLNSATELHSYIDKTQLTRDLGGTLDYCHTNWLSHRTAIENFAVMVKQTAQMLQSFGTELAETELPNDVPSTSLLLHAHTEKKEKMKDHLKIALTQGCDILNKIMEPRKENPEYRMNQDQLDNQTTVERLLLQLNETEAAFDEFWSKHEQKLEQCLQLRHFELNFREISTSLDGISERLEAFTDVGNSCSHAEHILKDFCNFEVKSREVLQTAQNLVREGDQLIKSNHYAVDSILPKCDEIGHLCDSISNTIQQRRQNLHKSLELHRHLEKTMKWCDEGIFLLASQPVDKFQSQDGAEAALQEITRFLETGTDITMKDLAFILKMYNSILTPELQEHVNKVYQKQESMQEMFEKRKGSLRKLAAKQTRPVQPVAPRPEAISKSPTSSPGLHRSSESRYSSGSIQRRINYRRAKSEVDGSQMRSTSVMEDEENLAVLRRHVMNELIETERAYVEELLSVIEGYAAEMNNPLVMDLIPSVLQNKKDVLFGNMEEIYQFHNRIFLRELEHYRDYPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDAAFFQDCQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALASILGILKAVNDSMHQIAITGYEGNLHELGKLLMQGSFNVWTEHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKRDENGEGYEKAPSYSYKHSLNMMFVGITEYVKGDNRKFEIWYNAREEVYIVQAPTTEVKATWVNEIRKVLTSQLKAHRDASHHKSAEHANSLPTPLPSSTSPLRNKSGKIKKLEERKTEPLLNPDVSLTSVPKYLEKGKGWNRASHSLDVSEENNEDWSSGEDPINSSDAEEDGGGDAPEKKLTPGKYTVVADYERGSDELLVKSGDLVHIIHEEDGGMWYVKNLSTNKEGLVPASNLMTLIGTSKSALSLSSSESGPASSTLSTSSSCSESCNAGTTSFSDIKG

Target ORF information:

RefSeq Version	XM_002933006.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933006.4

ORF Insert Sequence:

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGGTTGGAA CAGAGCATCA 
CATTCACTTG ATGTCTCTGA AGAAAATAAT GAAGATTGGT CTAGCGGAGA GGATCCAATT 
AACTCTTCAG ATGCAGAAGA AGATGGAGGA GGTGATGCAC CAGAGAAAAA GCTAACTCCT 
GGTAAATATA CTGTTGTGGC TGATTACGAG AGAGGTTCAG ATGAGCTGCT GGTAAAGAGT 
GGAGATCTTG TTCACATAAT ACATGAAGAG GATGGTGGAA TGTGGTATGT GAAGAACCTG 
AGTACCAATA AGGAAGGTCT GGTACCTGCT AGCAACCTAA TGACTCTCAT AGGCACTTCC 
AAATCTGCCC TCTCTTTAAG CAGCTCTGAA TCGGGACCCG CTTCAAGCAC TTTAAGCACA 
TCTTCTAGCT GTAGTGAGAG CTGCAATGCA GGCACCACCA GTTTCTCTGA TATCAAGGGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l ( XM_012957900.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813354.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa36977
Clone ID Related Accession (Same CDS sequence)	XM_012957900.2 , XM_012957901.2
Accession Version	XM_012957900.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957900.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCTTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG 
GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC 
TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT 
AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813354.1
CDS	678..4328
Translation	MSECWGLLICRALGSGNCFAAFRPCSEEEVDVNDHKVVVPDIIQLVPDDQSELELPSDDIMQQETFPICAADIEDQLRKQFAYLSGGRGENGCPVVTFPEYPAFSEIPEKEFQNVLTYLTSVPILQDAGIGFILVIDRRLDKWTSVKASILRIAASFPGNLQLVIVLRPSGLLQRTLSDIAFKFNKDDFKMKVPIIMLNSATELHSYIDKTQLTRDLGGTLDYCHTNWLSHRTAIENFAVMVKQTAQMLQSFGTELAETELPNDVPSTSLLLHAHTEKKEKMKDHLKIALTQGCDILNKIMEPRKENPEYRMNQDQLDNQTTVERLLLQLNETEAAFDEFWSKHEQKLEQCLQLRHFELNFREISTSLDGISERLEAFTDVGNSCSHAEHILKDFCNFEVKSREVLQTAQNLVREGDQLIKSNHYAVDSILPKCDEIGHLCDSISNTIQQRRQNLHKSLELHRHLEKTMKWCDEGIFLLASQPVDKFQSQDGAEAALQEITRFLETGTDITMKDLAFILKMYNSILTPELQEHVNKVYQKQESMQEMFEKRKGSLRKLAAKQTRPVQPVAPRPEAISKSPTSSPGLHRSSESRYSSGSIQRRINYRRAKSEVDGSQMRSTSVMEDEENLAVLRRHVMNELIETERAYVEELLSVIEGYAAEMNNPLVMDLIPSVLQNKKDVLFGNMEEIYQFHNRIFLRELEHYRDYPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDAAFFQDCQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALASILGILKAVNDSMHQIAITGYEGNLHELGKLLMQGSFNVWTEHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKRDENGEGYEKAPSYSYKHSLNMMFVGITEYVKGDNRKFEIWYNAREEVYIVQAPTTEVKATWVNEIRKVLTSQLKAHRDASHHKSAEHANSLPTPLPSSTSPLRNKSGKIKKLEERKTEPLLNPDVSLTSVPKYLEKGKDGTVTSPTSDSSALSKKRFTLQGFSNLKSQKASPTSPDKKAKRHEVKSDPTPFGLRGWNRASHSLDVSEENNEDWSSGEDPINSSDAEEDGGGDAPEKKLTPGKYTVVADYERGSDELLVKSGDLVHIIHEEDGGMWYVKNLSTNKEGLVPASNLMTLIGTSKSALSLSSSESGPASSTLSTSSSCSESCNAGTTSFSDIKG

Target ORF information:

RefSeq Version	XM_012957900.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957900.2

ORF Insert Sequence:

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCTTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG 
GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC 
TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT 
AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l ( XM_012957901.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813355.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa36977
Clone ID Related Accession (Same CDS sequence)	XM_012957900.2 , XM_012957901.2
Accession Version	XM_012957901.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957901.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCTTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG 
GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC 
TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT 
AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813355.1
CDS	28..3678
Translation	MSECWGLLICRALGSGNCFAAFRPCSEEEVDVNDHKVVVPDIIQLVPDDQSELELPSDDIMQQETFPICAADIEDQLRKQFAYLSGGRGENGCPVVTFPEYPAFSEIPEKEFQNVLTYLTSVPILQDAGIGFILVIDRRLDKWTSVKASILRIAASFPGNLQLVIVLRPSGLLQRTLSDIAFKFNKDDFKMKVPIIMLNSATELHSYIDKTQLTRDLGGTLDYCHTNWLSHRTAIENFAVMVKQTAQMLQSFGTELAETELPNDVPSTSLLLHAHTEKKEKMKDHLKIALTQGCDILNKIMEPRKENPEYRMNQDQLDNQTTVERLLLQLNETEAAFDEFWSKHEQKLEQCLQLRHFELNFREISTSLDGISERLEAFTDVGNSCSHAEHILKDFCNFEVKSREVLQTAQNLVREGDQLIKSNHYAVDSILPKCDEIGHLCDSISNTIQQRRQNLHKSLELHRHLEKTMKWCDEGIFLLASQPVDKFQSQDGAEAALQEITRFLETGTDITMKDLAFILKMYNSILTPELQEHVNKVYQKQESMQEMFEKRKGSLRKLAAKQTRPVQPVAPRPEAISKSPTSSPGLHRSSESRYSSGSIQRRINYRRAKSEVDGSQMRSTSVMEDEENLAVLRRHVMNELIETERAYVEELLSVIEGYAAEMNNPLVMDLIPSVLQNKKDVLFGNMEEIYQFHNRIFLRELEHYRDYPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDAAFFQDCQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALASILGILKAVNDSMHQIAITGYEGNLHELGKLLMQGSFNVWTEHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKRDENGEGYEKAPSYSYKHSLNMMFVGITEYVKGDNRKFEIWYNAREEVYIVQAPTTEVKATWVNEIRKVLTSQLKAHRDASHHKSAEHANSLPTPLPSSTSPLRNKSGKIKKLEERKTEPLLNPDVSLTSVPKYLEKGKDGTVTSPTSDSSALSKKRFTLQGFSNLKSQKASPTSPDKKAKRHEVKSDPTPFGLRGWNRASHSLDVSEENNEDWSSGEDPINSSDAEEDGGGDAPEKKLTPGKYTVVADYERGSDELLVKSGDLVHIIHEEDGGMWYVKNLSTNKEGLVPASNLMTLIGTSKSALSLSSSESGPASSTLSTSSSCSESCNAGTTSFSDIKG

Target ORF information:

RefSeq Version	XM_012957901.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957901.2

ORF Insert Sequence:

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCTTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG 
GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC 
TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT 
AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l ( XM_012957903.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813357.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X3, mRNA.

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CloneID	OXa36979
Clone ID Related Accession (Same CDS sequence)	XM_012957903.2
Accession Version	XM_012957903.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957903.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTG TAAAAATTTC AGTGGAAGAA ATGAAAGTCT ACCATTGGTT TTCTGAGATG 
TGCTTAATGC TCCATTCTTC GGTGCACAAT GACATCATGC AACAGGAGAC CTTTCCAATC 
TGTGCAGCCG ATATTGAGGA TCAGCTAAGA AAGCAGTTTG CCTACTTGTC AGGTGGAAGA 
GGGGAAAATG GCTGTCCAGT CGTCACATTC CCTGAATACC CAGCATTTAG CGAGATTCCA 
GAAAAAGAGT TCCAGAATGT ACTCACCTAT CTGACAAGTG TCCCAATTTT ACAAGATGCG 
GGAATTGGCT TCATCTTAGT GATAGACAGA AGGCTGGACA AATGGACCTC AGTGAAAGCC 
TCTATCCTGA GGATTGCGGC ATCTTTTCCT GGGAATTTAC AGCTTGTTAT AGTCCTGCGC 
CCATCAGGAC TATTGCAGCG CACCCTCTCG GATATTGCAT TCAAATTCAA CAAAGATGAC 
TTTAAAATGA AAGTACCGAT CATAATGCTG AATTCAGCAA CAGAACTCCA TAGTTATATT 
GACAAGACCC AGCTGACTCG AGATCTTGGT GGCACTCTGG ATTACTGCCA TACTAACTGG 
CTCTCACATC GCACTGCTAT TGAAAACTTT GCTGTCATGG TGAAACAAAC AGCCCAAATG 
CTCCAGTCTT TTGGGACAGA GCTGGCAGAG ACAGAGCTGC CAAATGATGT TCCCTCTACA 
AGCTTGCTGC TCCACGCACA CACAGAGAAA AAGGAAAAAA TGAAGGATCA CCTTAAGATA 
GCACTGACAC AGGGATGTGA CATTTTGAAC AAGATCATGG AGCCACGTAA AGAGAACCCT 
GAGTATAGGA TGAATCAGGA CCAACTGGAT AACCAAACTA CTGTGGAAAG GCTTCTTTTG 
CAATTAAATG AAACAGAAGC TGCTTTTGAT GAGTTTTGGT CTAAACATGA ACAGAAGCTT 
GAACAGTGTT TACAGCTGCG TCATTTTGAG TTAAACTTCA GAGAGATTTC TACAAGTCTG 
GATGGTATTT CTGAGAGGCT AGAAGCTTTT ACAGATGTTG GAAACAGCTG CTCCCATGCT 
GAACACATCC TGAAGGACTT CTGTAACTTT GAAGTTAAAT CCCGAGAAGT TTTACAGACA 
GCCCAGAATT TAGTCAGAGA AGGTGATCAA CTCATTAAAA GTAATCACTA TGCTGTGGAC 
TCCATTCTTC CAAAATGCGA CGAGATTGGT CACTTATGTG ACAGCATTAG CAACACTATT 
CAGCAGAGAC GGCAAAACCT TCACAAATCC TTGGAACTTC ACAGACATTT GGAGAAGACA 
ATGAAGTGGT GTGATGAAGG GATTTTCTTG CTGGCGTCCC AACCTGTGGA TAAGTTCCAG 
TCTCAGGATG GGGCCGAAGC TGCACTTCAG GAGATTACAA GGTTTCTAGA GACTGGAACA 
GATATAACCA TGAAAGATTT GGCTTTCATT TTGAAGATGT ACAACTCGAT TCTCACTCCA 
GAACTCCAGG AACATGTCAA TAAGGTATAC CAGAAGCAGG AAAGCATGCA GGAGATGTTT 
GAGAAAAGGA AAGGTAGCCT TAGAAAACTT GCAGCAAAGC AAACCAGACC TGTCCAGCCT 
GTTGCACCTA GACCAGAAGC AATTTCCAAG TCTCCTACCT CCTCTCCAGG TCTTCATAGG 
TCTTCTGAAA GCAGATATAG CTCAGGAAGT ATTCAACGAC GGATAAATTA TCGCAGAGCC 
AAGAGCGAAG TGGATGGTAG TCAGATGAGA AGTACATCAG TGATGGAAGA CGAAGAAAAT 
CTAGCAGTGT TAAGAAGACA TGTCATGAAT GAACTTATTG AGACAGAAAG GGCATATGTG 
GAAGAACTTC TATCTGTGAT TGAGGGTTAT GCTGCTGAAA TGAATAATCC TCTAGTGATG 
GACCTTATCC CCTCAGTGCT CCAGAATAAG AAGGATGTTC TTTTCGGCAA CATGGAAGAA 
ATTTACCAGT TTCATAACAG AATATTTCTG AGAGAGCTGG AACATTATAG AGACTATCCA 
GAACTAGTGG GAAGGTGTTT TTTGGAAAGG ATGGAAGATT TCCAGATCTA TGAAAAGTAC 
TGCCAGAACA AGCCACGGTC AGAGAGTTTG TGGAGGCAAT GCTCCGATGC TGCTTTCTTC 
CAGGATTGCC AAAAGAAACT GGACCATAAA CTCAGCCTGG ATTCCTATCT TCTAAAGCCG 
GTGCAAAGGA TAACAAAGTA TCAGCTTCTG TTAAAGGAAA TGCTTAAGTA TAGTAAAAAC 
TGTGAAGGAG CTGAAGATCT TCAGGAAGCA CTTGCCTCTA TATTGGGCAT TCTTAAAGCT 
GTCAATGATT CCATGCATCA GATAGCAATC ACTGGATATG AGGGGAACTT ACATGAACTT 
GGTAAACTCT TAATGCAAGG ATCATTTAAT GTATGGACAG AGCACAAGAA GGGCCATTCC 
AAAGTAAAGG ACCTTGCTAG GTTTAAACCA ATGCAAAGAC ACCTGTTTCT GCATGAAAAA 
GCTGTGCTGT TCTGCAAGAA GAGGGATGAG AATGGGGAGG GCTATGAGAA GGCACCATCC 
TACAGCTACA AACATTCCTT GAATATGATG TTTGTTGGAA TTACTGAGTA TGTAAAAGGA 
GACAATAGGA AATTTGAAAT TTGGTACAAC GCAAGGGAGG AGGTTTATAT TGTGCAGGCA 
CCAACCACAG AAGTAAAGGC CACCTGGGTA AATGAAATCA GAAAGGTGCT GACAAGCCAG 
TTAAAGGCAC ACAGAGATGC CAGCCACCAT AAATCGGCAG AGCACGCAAA CAGCTTACCT 
ACTCCTCTCC CATCCAGTAC TAGTCCCCTG AGAAACAAAT CTGGGAAGAT CAAAAAGCTG 
GAGGAAAGAA AAACTGAGCC TTTATTGAAT CCGGATGTCT CCTTAACATC TGTACCAAAA 
TACCTTGAAA AAGGCAAAGA TGGCACAGTA ACTAGTCCTA CCTCAGACTC TTCTGCCTTG 
TCAAAAAAGC GGTTCACTTT GCAGGGTTTT TCAAACCTCA AAAGTCAGAA AGCTTCTCCT 
ACCAGCCCAG ACAAAAAAGC TAAACGCCAT GAAGTAAAGA GTGACCCAAC TCCCTTTGGT 
TTAAGAGGTT GGAACAGAGC ATCACATTCA CTTGATGTCT CTGAAGAAAA TAATGAAGAT 
TGGTCTAGCG GAGAGGATCC AATTAACTCT TCAGATGCAG AAGAAGATGG AGGAGGTGAT 
GCACCAGAGA AAAAGCTAAC TCCTGGTAAA TATACTGTTG TGGCTGATTA CGAGAGAGGT 
TCAGATGAGC TGCTGGTAAA GAGTGGAGAT CTTGTTCACA TAATACATGA AGAGGATGGT 
GGAATGTGGT ATGTGAAGAA CCTGAGTACC AATAAGGAAG GTCTGGTACC TGCTAGCAAC 
CTAATGACTC TCATAGGCAC TTCCAAATCT GCCCTCTCTT TAAGCAGCTC TGAATCGGGA 
CCCGCTTCAA GCACTTTAAG CACATCTTCT AGCTGTAGTG AGAGCTGCAA TGCAGGCACC 
ACCAGTTTCT CTGATATCAA GGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813357.1
CDS	195..3761
Translation	MDCVKISVEEMKVYHWFSEMCLMLHSSVHNDIMQQETFPICAADIEDQLRKQFAYLSGGRGENGCPVVTFPEYPAFSEIPEKEFQNVLTYLTSVPILQDAGIGFILVIDRRLDKWTSVKASILRIAASFPGNLQLVIVLRPSGLLQRTLSDIAFKFNKDDFKMKVPIIMLNSATELHSYIDKTQLTRDLGGTLDYCHTNWLSHRTAIENFAVMVKQTAQMLQSFGTELAETELPNDVPSTSLLLHAHTEKKEKMKDHLKIALTQGCDILNKIMEPRKENPEYRMNQDQLDNQTTVERLLLQLNETEAAFDEFWSKHEQKLEQCLQLRHFELNFREISTSLDGISERLEAFTDVGNSCSHAEHILKDFCNFEVKSREVLQTAQNLVREGDQLIKSNHYAVDSILPKCDEIGHLCDSISNTIQQRRQNLHKSLELHRHLEKTMKWCDEGIFLLASQPVDKFQSQDGAEAALQEITRFLETGTDITMKDLAFILKMYNSILTPELQEHVNKVYQKQESMQEMFEKRKGSLRKLAAKQTRPVQPVAPRPEAISKSPTSSPGLHRSSESRYSSGSIQRRINYRRAKSEVDGSQMRSTSVMEDEENLAVLRRHVMNELIETERAYVEELLSVIEGYAAEMNNPLVMDLIPSVLQNKKDVLFGNMEEIYQFHNRIFLRELEHYRDYPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDAAFFQDCQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALASILGILKAVNDSMHQIAITGYEGNLHELGKLLMQGSFNVWTEHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKRDENGEGYEKAPSYSYKHSLNMMFVGITEYVKGDNRKFEIWYNAREEVYIVQAPTTEVKATWVNEIRKVLTSQLKAHRDASHHKSAEHANSLPTPLPSSTSPLRNKSGKIKKLEERKTEPLLNPDVSLTSVPKYLEKGKDGTVTSPTSDSSALSKKRFTLQGFSNLKSQKASPTSPDKKAKRHEVKSDPTPFGLRGWNRASHSLDVSEENNEDWSSGEDPINSSDAEEDGGGDAPEKKLTPGKYTVVADYERGSDELLVKSGDLVHIIHEEDGGMWYVKNLSTNKEGLVPASNLMTLIGTSKSALSLSSSESGPASSTLSTSSSCSESCNAGTTSFSDIKG

Target ORF information:

RefSeq Version	XM_012957903.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957903.2

ORF Insert Sequence:

ATGGATTGTG TAAAAATTTC AGTGGAAGAA ATGAAAGTCT ACCATTGGTT TTCTGAGATG 
TGCTTAATGC TCCATTCTTC GGTGCACAAT GACATCATGC AACAGGAGAC CTTTCCAATC 
TGTGCAGCCG ATATTGAGGA TCAGCTAAGA AAGCAGTTTG CCTACTTGTC AGGTGGAAGA 
GGGGAAAATG GCTGTCCAGT CGTCACATTC CCTGAATACC CAGCATTTAG CGAGATTCCA 
GAAAAAGAGT TCCAGAATGT ACTCACCTAT CTGACAAGTG TCCCAATTTT ACAAGATGCG 
GGAATTGGCT TCATCTTAGT GATAGACAGA AGGCTGGACA AATGGACCTC AGTGAAAGCC 
TCTATCCTGA GGATTGCGGC ATCTTTTCCT GGGAATTTAC AGCTTGTTAT AGTCCTGCGC 
CCATCAGGAC TATTGCAGCG CACCCTCTCG GATATTGCAT TCAAATTCAA CAAAGATGAC 
TTTAAAATGA AAGTACCGAT CATAATGCTG AATTCAGCAA CAGAACTCCA TAGTTATATT 
GACAAGACCC AGCTGACTCG AGATCTTGGT GGCACTCTGG ATTACTGCCA TACTAACTGG 
CTCTCACATC GCACTGCTAT TGAAAACTTT GCTGTCATGG TGAAACAAAC AGCCCAAATG 
CTCCAGTCTT TTGGGACAGA GCTGGCAGAG ACAGAGCTGC CAAATGATGT TCCCTCTACA 
AGCTTGCTGC TCCACGCACA CACAGAGAAA AAGGAAAAAA TGAAGGATCA CCTTAAGATA 
GCACTGACAC AGGGATGTGA CATTTTGAAC AAGATCATGG AGCCACGTAA AGAGAACCCT 
GAGTATAGGA TGAATCAGGA CCAACTGGAT AACCAAACTA CTGTGGAAAG GCTTCTTTTG 
CAATTAAATG AAACAGAAGC TGCTTTTGAT GAGTTTTGGT CTAAACATGA ACAGAAGCTT 
GAACAGTGTT TACAGCTGCG TCATTTTGAG TTAAACTTCA GAGAGATTTC TACAAGTCTG 
GATGGTATTT CTGAGAGGCT AGAAGCTTTT ACAGATGTTG GAAACAGCTG CTCCCATGCT 
GAACACATCC TGAAGGACTT CTGTAACTTT GAAGTTAAAT CCCGAGAAGT TTTACAGACA 
GCCCAGAATT TAGTCAGAGA AGGTGATCAA CTCATTAAAA GTAATCACTA TGCTGTGGAC 
TCCATTCTTC CAAAATGCGA CGAGATTGGT CACTTATGTG ACAGCATTAG CAACACTATT 
CAGCAGAGAC GGCAAAACCT TCACAAATCC TTGGAACTTC ACAGACATTT GGAGAAGACA 
ATGAAGTGGT GTGATGAAGG GATTTTCTTG CTGGCGTCCC AACCTGTGGA TAAGTTCCAG 
TCTCAGGATG GGGCCGAAGC TGCACTTCAG GAGATTACAA GGTTTCTAGA GACTGGAACA 
GATATAACCA TGAAAGATTT GGCTTTCATT TTGAAGATGT ACAACTCGAT TCTCACTCCA 
GAACTCCAGG AACATGTCAA TAAGGTATAC CAGAAGCAGG AAAGCATGCA GGAGATGTTT 
GAGAAAAGGA AAGGTAGCCT TAGAAAACTT GCAGCAAAGC AAACCAGACC TGTCCAGCCT 
GTTGCACCTA GACCAGAAGC AATTTCCAAG TCTCCTACCT CCTCTCCAGG TCTTCATAGG 
TCTTCTGAAA GCAGATATAG CTCAGGAAGT ATTCAACGAC GGATAAATTA TCGCAGAGCC 
AAGAGCGAAG TGGATGGTAG TCAGATGAGA AGTACATCAG TGATGGAAGA CGAAGAAAAT 
CTAGCAGTGT TAAGAAGACA TGTCATGAAT GAACTTATTG AGACAGAAAG GGCATATGTG 
GAAGAACTTC TATCTGTGAT TGAGGGTTAT GCTGCTGAAA TGAATAATCC TCTAGTGATG 
GACCTTATCC CCTCAGTGCT CCAGAATAAG AAGGATGTTC TTTTCGGCAA CATGGAAGAA 
ATTTACCAGT TTCATAACAG AATATTTCTG AGAGAGCTGG AACATTATAG AGACTATCCA 
GAACTAGTGG GAAGGTGTTT TTTGGAAAGG ATGGAAGATT TCCAGATCTA TGAAAAGTAC 
TGCCAGAACA AGCCACGGTC AGAGAGTTTG TGGAGGCAAT GCTCCGATGC TGCTTTCTTC 
CAGGATTGCC AAAAGAAACT GGACCATAAA CTCAGCCTGG ATTCCTATCT TCTAAAGCCG 
GTGCAAAGGA TAACAAAGTA TCAGCTTCTG TTAAAGGAAA TGCTTAAGTA TAGTAAAAAC 
TGTGAAGGAG CTGAAGATCT TCAGGAAGCA CTTGCCTCTA TATTGGGCAT TCTTAAAGCT 
GTCAATGATT CCATGCATCA GATAGCAATC ACTGGATATG AGGGGAACTT ACATGAACTT 
GGTAAACTCT TAATGCAAGG ATCATTTAAT GTATGGACAG AGCACAAGAA GGGCCATTCC 
AAAGTAAAGG ACCTTGCTAG GTTTAAACCA ATGCAAAGAC ACCTGTTTCT GCATGAAAAA 
GCTGTGCTGT TCTGCAAGAA GAGGGATGAG AATGGGGAGG GCTATGAGAA GGCACCATCC 
TACAGCTACA AACATTCCTT GAATATGATG TTTGTTGGAA TTACTGAGTA TGTAAAAGGA 
GACAATAGGA AATTTGAAAT TTGGTACAAC GCAAGGGAGG AGGTTTATAT TGTGCAGGCA 
CCAACCACAG AAGTAAAGGC CACCTGGGTA AATGAAATCA GAAAGGTGCT GACAAGCCAG 
TTAAAGGCAC ACAGAGATGC CAGCCACCAT AAATCGGCAG AGCACGCAAA CAGCTTACCT 
ACTCCTCTCC CATCCAGTAC TAGTCCCCTG AGAAACAAAT CTGGGAAGAT CAAAAAGCTG 
GAGGAAAGAA AAACTGAGCC TTTATTGAAT CCGGATGTCT CCTTAACATC TGTACCAAAA 
TACCTTGAAA AAGGCAAAGA TGGCACAGTA ACTAGTCCTA CCTCAGACTC TTCTGCCTTG 
TCAAAAAAGC GGTTCACTTT GCAGGGTTTT TCAAACCTCA AAAGTCAGAA AGCTTCTCCT 
ACCAGCCCAG ACAAAAAAGC TAAACGCCAT GAAGTAAAGA GTGACCCAAC TCCCTTTGGT 
TTAAGAGGTT GGAACAGAGC ATCACATTCA CTTGATGTCT CTGAAGAAAA TAATGAAGAT 
TGGTCTAGCG GAGAGGATCC AATTAACTCT TCAGATGCAG AAGAAGATGG AGGAGGTGAT 
GCACCAGAGA AAAAGCTAAC TCCTGGTAAA TATACTGTTG TGGCTGATTA CGAGAGAGGT 
TCAGATGAGC TGCTGGTAAA GAGTGGAGAT CTTGTTCACA TAATACATGA AGAGGATGGT 
GGAATGTGGT ATGTGAAGAA CCTGAGTACC AATAAGGAAG GTCTGGTACC TGCTAGCAAC 
CTAATGACTC TCATAGGCAC TTCCAAATCT GCCCTCTCTT TAAGCAGCTC TGAATCGGGA 
CCCGCTTCAA GCACTTTAAG CACATCTTCT AGCTGTAGTG AGAGCTGCAA TGCAGGCACC 
ACCAGTTTCT CTGATATCAA GGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l ( XM_012957904.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813358.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa36980
Clone ID Related Accession (Same CDS sequence)	XM_012957904.2
Accession Version	XM_012957904.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957904.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGCTTCTCC TACCAGCCCA 
GACAAAAAAG CTAAACGCCA TGAAGTAAAG AGTGACCCAA CTCCCTTTGG TTTAAGAGGT 
TGGAACAGAG CATCACATTC ACTTGATGTC TCTGAAGAAA ATAATGAAGA TTGGTCTAGC 
GGAGAGGATC CAATTAACTC TTCAGATGCA GAAGAAGATG GAGGAGGTGA TGCACCAGAG 
AAAAAGCTAA CTCCTGGTAA ATATACTGTT GTGGCTGATT ACGAGAGAGG TTCAGATGAG 
CTGCTGGTAA AGAGTGGAGA TCTTGTTCAC ATAATACATG AAGAGGATGG TGGAATGTGG 
TATGTGAAGA ACCTGAGTAC CAATAAGGAA GGTCTGGTAC CTGCTAGCAA CCTAATGACT 
CTCATAGGCA CTTCCAAATC TGCCCTCTCT TTAAGCAGCT CTGAATCGGG ACCCGCTTCA 
AGCACTTTAA GCACATCTTC TAGCTGTAGT GAGAGCTGCA ATGCAGGCAC CACCAGTTTC 
TCTGATATCA AGGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813358.1
CDS	672..4229
Translation	MSECWGLLICRALGSGNCFAAFRPCSEEEVDVNDHKVVVPDIIQLVPDDQSELELPSDDIMQQETFPICAADIEDQLRKQFAYLSGGRGENGCPVVTFPEYPAFSEIPEKEFQNVLTYLTSVPILQDAGIGFILVIDRRLDKWTSVKASILRIAASFPGNLQLVIVLRPSGLLQRTLSDIAFKFNKDDFKMKVPIIMLNSATELHSYIDKTQLTRDLGGTLDYCHTNWLSHRTAIENFAVMVKQTAQMLQSFGTELAETELPNDVPSTSLLLHAHTEKKEKMKDHLKIALTQGCDILNKIMEPRKENPEYRMNQDQLDNQTTVERLLLQLNETEAAFDEFWSKHEQKLEQCLQLRHFELNFREISTSLDGISERLEAFTDVGNSCSHAEHILKDFCNFEVKSREVLQTAQNLVREGDQLIKSNHYAVDSILPKCDEIGHLCDSISNTIQQRRQNLHKSLELHRHLEKTMKWCDEGIFLLASQPVDKFQSQDGAEAALQEITRFLETGTDITMKDLAFILKMYNSILTPELQEHVNKVYQKQESMQEMFEKRKGSLRKLAAKQTRPVQPVAPRPEAISKSPTSSPGLHRSSESRYSSGSIQRRINYRRAKSEVDGSQMRSTSVMEDEENLAVLRRHVMNELIETERAYVEELLSVIEGYAAEMNNPLVMDLIPSVLQNKKDVLFGNMEEIYQFHNRIFLRELEHYRDYPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDAAFFQDCQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALASILGILKAVNDSMHQIAITGYEGNLHELGKLLMQGSFNVWTEHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKRDENGEGYEKAPSYSYKHSLNMMFVGITEYVKGDNRKFEIWYNAREEVYIVQAPTTEVKATWVNEIRKVLTSQLKAHRDASHHKSAEHANSLPTPLPSSTSPLRNKSGKIKKLEERKTEPLLNPDVSLTSVPKYLEKGKASPTSPDKKAKRHEVKSDPTPFGLRGWNRASHSLDVSEENNEDWSSGEDPINSSDAEEDGGGDAPEKKLTPGKYTVVADYERGSDELLVKSGDLVHIIHEEDGGMWYVKNLSTNKEGLVPASNLMTLIGTSKSALSLSSSESGPASSTLSTSSSCSESCNAGTTSFSDIKG

Target ORF information:

RefSeq Version	XM_012957904.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957904.2

ORF Insert Sequence:

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGCTTCTCC TACCAGCCCA 
GACAAAAAAG CTAAACGCCA TGAAGTAAAG AGTGACCCAA CTCCCTTTGG TTTAAGAGGT 
TGGAACAGAG CATCACATTC ACTTGATGTC TCTGAAGAAA ATAATGAAGA TTGGTCTAGC 
GGAGAGGATC CAATTAACTC TTCAGATGCA GAAGAAGATG GAGGAGGTGA TGCACCAGAG 
AAAAAGCTAA CTCCTGGTAA ATATACTGTT GTGGCTGATT ACGAGAGAGG TTCAGATGAG 
CTGCTGGTAA AGAGTGGAGA TCTTGTTCAC ATAATACATG AAGAGGATGG TGGAATGTGG 
TATGTGAAGA ACCTGAGTAC CAATAAGGAA GGTCTGGTAC CTGCTAGCAA CCTAATGACT 
CTCATAGGCA CTTCCAAATC TGCCCTCTCT TTAAGCAGCT CTGAATCGGG ACCCGCTTCA 
AGCACTTTAA GCACATCTTC TAGCTGTAGT GAGAGCTGCA ATGCAGGCAC CACCAGTTTC 
TCTGATATCA AGGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l ( XM_012957906.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813360.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X6, mRNA.

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CloneID	OXa36982
Clone ID Related Accession (Same CDS sequence)	XM_012957906.2
Accession Version	XM_012957906.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957906.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCTTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813360.1
CDS	684..4136
Translation	MSECWGLLICRALGSGNCFAAFRPCSEEEVDVNDHKVVVPDIIQLVPDDQSELELPSDDIMQQETFPICAADIEDQLRKQFAYLSGGRGENGCPVVTFPEYPAFSEIPEKEFQNVLTYLTSVPILQDAGIGFILVIDRRLDKWTSVKASILRIAASFPGNLQLVIVLRPSGLLQRTLSDIAFKFNKDDFKMKVPIIMLNSATELHSYIDKTQLTRDLGGTLDYCHTNWLSHRTAIENFAVMVKQTAQMLQSFGTELAETELPNDVPSTSLLLHAHTEKKEKMKDHLKIALTQGCDILNKIMEPRKENPEYRMNQDQLDNQTTVERLLLQLNETEAAFDEFWSKHEQKLEQCLQLRHFELNFREISTSLDGISERLEAFTDVGNSCSHAEHILKDFCNFEVKSREVLQTAQNLVREGDQLIKSNHYAVDSILPKCDEIGHLCDSISNTIQQRRQNLHKSLELHRHLEKTMKWCDEGIFLLASQPVDKFQSQDGAEAALQEITRFLETGTDITMKDLAFILKMYNSILTPELQEHVNKVYQKQESMQEMFEKRKGSLRKLAAKQTRPVQPVAPRPEAISKSPTSSPGLHRSSESRYSSGSIQRRINYRRAKSEVDGSQMRSTSVMEDEENLAVLRRHVMNELIETERAYVEELLSVIEGYAAEMNNPLVMDLIPSVLQNKKDVLFGNMEEIYQFHNRIFLRELEHYRDYPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDAAFFQDCQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALASILGILKAVNDSMHQIAITGYEGNLHELGKLLMQGSFNVWTEHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKRDENGEGYEKAPSYSYKHSLNMMFVGITEYVKGDNRKFEIWYNAREEVYIVQAPTTEVKATWVNEIRKVLTSQLKAHRDASHHKSAEHANSLPTPLPSSTSPLRNKSGKIKKLEERKTEPLLNPDVSLTSVPKYLEKGKDGTVTSPTSDSSALSKKRFTLQGFSNLKSQKASPTSPDKKAKRHEVKSDPTPFGLRGWNRASHSLDVSEENNEDWSSGEDPINSSDAEEDGGGDAPEKKLTPGKYTVVADYERGSDELLVKSGDLVHIIHEEDGGM

Target ORF information:

RefSeq Version	XM_012957906.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957906.2

ORF Insert Sequence:

ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT 
GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC 
ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA 
TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA 
AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG 
GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT 
TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT 
GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA 
GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT 
CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC 
ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG 
CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA 
AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC 
ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA 
ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT 
TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC 
TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT 
GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT 
AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT 
AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA 
TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA 
CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG 
TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT 
ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG 
ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG 
CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA 
AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT 
ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA 
CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA 
TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT 
ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT 
GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT 
GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG 
CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA 
GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG 
CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC 
CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG 
GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC 
TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA 
TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG 
ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA 
AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG 
GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT 
GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG 
GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA 
ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG 
GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC 
AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT 
GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT 
CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC 
CTCAAAAGTC AGAAAGCTTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA 
AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT 
GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT 
GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT 
GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT 
CACATAATAC ATGAAGAGGA TGGTGGAATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	mcf2l
Entrez Gene ID	100497730
Full Name	MCF.2 cell line derived transforming sequence-like
Synonyms	arhgef14,dbs,ost
General protein information	Preferred Names MCF.2 cell line derived transforming sequence-like Names guanine nucleotide exchange factor DBS
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Xenopus tropicalis (tropical clawed frog)	LOC100497730	XP_002933052.2
Homo sapiens (human)	MCF2L	NP_001106203.2
Pan troglodytes (chimpanzee)	MCF2L	XP_003314263.1
	MCF2L	XP_001103476.2
Mus musculus (house mouse)	Mcf2l	NP_835177.2
Rattus norvegicus (Norway rat)	Mcf2l	NP_446403.2
Canis lupus familiaris (dog)	MCF2L	XP_005634173.1
Gallus gallus (chicken)	MCF2L	NP_001238992.1
Danio rerio (zebrafish)	mcf2la	XP_005171065.1
Bos taurus (cattle)	MCF2L	XP_005214085.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

mcf2l cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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