Gli2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gli2
Entrez Gene ID	14633
Full Name	GLI-Kruppel family member GLI2
General protein information	Preferred Names GLI-Kruppel family member GLI2 Names zinc finger protein GLI2 Tax helper protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 E2.3\|1 52.17 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_030248278.1	XP_030104138.1	zinc finger protein GLI2 isoform X1
XM_011247924.2	XP_011246226.1	zinc finger protein GLI2 isoform X1
NM_001081125.1	NP_001074594.1	zinc finger protein GLI2
XM_006529141.3	XP_006529204.1	zinc finger protein GLI2 isoform X1
XM_006529141.4	XP_006529204.1	zinc finger protein GLI2 isoform X2
XM_011247924.3	XP_011246226.1	zinc finger protein GLI2 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gli2	NP_001074594.1
Xenopus tropicalis (tropical clawed frog)	LOC100498653	XP_002943329.2
Homo sapiens (human)	GLI2	NP_005261.2
Canis lupus familiaris (dog)	GLI2	XP_003432520.1
Rattus norvegicus (Norway rat)	Gli2	NP_001100639.1
Danio rerio (zebrafish)	gli2a	NP_571042.1
Bos taurus (cattle)	GLI2	NP_001179179.2
Pan troglodytes (chimpanzee)	GLI2	XP_001158280.2
	LOC705877	XP_002808099.1
Gallus gallus (chicken)	GLI2	NP_001258830.1

Gene Ontology

The following Gli2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gli2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu27904	XM_011247924.3 Latest version!	Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu27904	XM_006529141.4 Latest version!	Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu22271	XM_030248278.1 Latest version!	Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu22271	NM_001081125.1 Latest version!	Mus musculus GLI-Kruppel family member GLI2 (Gli2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$909.30 $1299.00
View Old Accession Versions >>
OMu27904	XM_006529141.3	Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu27904	XM_011247924.2	Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gli2 ( XM_006529141.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529204.1 Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu27904
Clone ID Related Accession (Same CDS sequence)	XM_011247924.2 , XM_006529141.3 , XM_006529141.4 , XM_011247924.3
Accession Version	XM_006529141.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	zinc finger protein GLI2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529141.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACACC AGGAGGGAAG GTACCATTAT GACCCTCACT CTGTCCACAG TGTACACGGG 
CCTCCCACCC TAAGTGGCAG CCCTGTCATC TCAGATATCT CCTTGATACG ACTTTCTCCA 
CACCCTGCTG GCCCTGGAGA GTCACCCTTC AGCGCCCACC ACCCCTACGT GAACCCCCAT 
ATGGAGCACT ACCTCCGGTC TGTGCACAGC AGCCCCACAC TCTCAATGAT CTCTGCCGCC 
AGGGGCCTCA GCCCTGCTGA TGTGGCCCAC GAACATCTGA AAGAGAGGGG ACTCTTTAGC 
CTCGCAGCCC CAGGCACCAA CCCTTCAGAC TATTACCACC AGATGACCCT CATGGCAAGC 
CACCCCACCC CTTATGGGGA CCTTCTAATG CAGAGCGGGG GTGCTGCTAG CGCACCCCAT 
CTCCATGACT ACCTCAACCC TGTGGATGCA TCACGATTCT CTAGTCCACG TGTGACCCCA 
CGACTGAGCC GCAAGCGGGC TCTGTCCATC TCCCCGCTCT CAGATGCCAG CCTCGACCTA 
CAACGCATGA TTCGGACCTC TCCCAACTCG CTGGTAGCTT ACATCAACAA CTCCAGGAGC 
AGCTCAGCAG CCAGTGGCTC TTATGGACAT CTGTCTGCTG GTGCCCTCAG CCCAGCCTTC 
ACTTTTCCCC ACCCCATCAA TCCTGTGGCC TACCAGCAGA TCCTGAGCCA GCAGCGGGGC 
CTGGGCTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CTTCACCCAC CTTCTTGGCC 
CAGCAGCCCA TGACTCTCAC CTCCATCAGC ACCATGCCTA CCCAACTCAG CAGCAGTAGC 
AGCAACTGTC TAAATGATGC CAACCAGAAC AAGCAGAACA GCGAGTCAGC TGTGAGCAGC 
ACCGTGAACC CCATCACCAT TCATAAGCGG AGCAAGGTCA AGACTGAGGC TGAGGGCCTG 
CGTCCAGCAT CCCCGCTTGG ACTGACACAG GAGCAGCTGG CCGATCTCAA GGAAGACCTG 
GACAGGGATG ACTGTAAGCA GGAGGCCGAG GTGGTCATCT ACGAGACCAA CTGCCACTGG 
GCAGACTGCA CCAAGGAGTA TGACACACAG GAGCAGCTGG TGCATCATAT CAACAATGAA 
CACATCCACG GGGAGAAGAA GGAGTTCGTG TGCCGCTGGC AGGCCTGCAC GAGAGAGCAG 
AAGCCCTTCA AGGCCCAGTA CATGCTGGTT GTTCACATGC GCAGACACAC GGGTGAGAAG 
CCACACAAGT GCACGTTCGA AGGCTGTTCC AAGGCCTACT CTCGCCTGGA GAACCTGAAG 
ACACACCTGC GTTCACACAC AGGAGAGAAG CCATATGTGT GTGAACACGA AGGCTGTAAC 
AAAGCCTTCT CCAATGCCTC AGACCGCGCC AAGCACCAGA ACCGCACTCA CTCCAATGAG 
AAACCCTACA TCTGCAAGAT CCCAGGCTGC ACCAAGAGGT ACACAGACCC CAGCTCACTC 
CGCAAGCATG TGAAGACTGT CCATGGGCCA GACGCCCATG TCACCAAGAA ACAGCGTAAT 
GATGTGCATG TCCGTGCTCC ACTGCTCAAG GAGAATGGGG ATAATGAGGC CAGCGCCGAG 
CCAGGTGGCC GGGGACCTGA GGAGAGTGTG GAGGCCAGTA GCACCAGCCA CACTGTGGAG 
GACTGCCTAC ATATCAAAGC CATCAAGACA GAGAGCTCCG GGCTTTGTCA GTCCAGCCCC 
GGGGCCCAGT CATCCTGCAG CAGCGAGCCC TCTCCCCTGG GCAGTGCCCC CAACAATGAC 
AGTGGCATGG AGATGCCGGG GACAGGGCCT GGGAGTCTGG GAGACCTGAC AGCACTGGCT 
GACACGTGTC CAGGAGCTGA CACCTCAGCC CTGGCTGCAC CCTCCACTGG TGGCCTGCAG 
CTGCGCAAAC ACATGAGCAC CGTGCATCGC TTTGAGCAGC TGAAGAGAGA GAAGCTCAAG 
TCACTGAAGG ATTCCTGCTC GTGGGCCGGC CCAGCTCCAC ACACCCGCAA CACCAAGCTG 
CCTCCCCTTC CAGTCAATGG TTCTGTCCTG GAAAACTTCA ACAATACAGG GGGCGGTGGA 
CCGGCAGGAC TGCTGCCCAG CCAGCGGCTA CCAGAGCTGA CCGAAGTGAC GATGCTGAGC 
CAGCTGCAGG AACGAAGAGA CAGCTCCACC AGCACCATGA GCTCGGCCTA CACTGTGAGC 
CGCCGCTCCT CTGGCATCTC CCCATACTTC TCTAGCCGTC GCTCCAGCGA GGCTTCGCCT 
CTCGGTGGCC TACGCCCGCA CAACGCCAGC TCAGCAGACT CCTATGACCC CATCTCCACA 
GATGCCTCTC GGCGCTCCAG TGAAGCCAGC CAGTGCAGTG GCGGTGGCCC AGGGCTGCTC 
AACCTCACAC CTGCGCAGCA GTACAACCTG CGTGCCAAGT ACGCAGCGGC CACAGGTGGA 
CCACCGCCCA CGCCACTGCC GGGCCTCGAT CGTGTAAGCC TTCGTACCCG CCTGGCCTTG 
CTGGATGCTC CTGAGCGTGC ACTTCCTGGT GCCTGCCCAC ATCCACTGGG GCCACGGCGT 
GGCAGCGATG GGCCTACCTA TAGCCATGGT CATGGCCATG GCTACGCAGG TGCGGCTCCA 
GCATTCCCCC ACGAGGGGCC AAACAGCAGC ACACGGCGGG CCAGCGACCC TGTGCGGCGC 
CCTGACCCCC TTATTCTGCC TCGAGTGCAA CGTTTCCACA GTACCCACAA CATGAATCCA 
GGTTCACTGC CACCCTGCGC TGATCGGCGT GGCCTGCACG TACAGAGCCA CCCCAGCGTA 
GACAGCAACC TGACCCGCAA CGCCTACTCT CCCAGACCCC CTAGCATCAA TGAGAACGTG 
GTGATGGAGG CCGTGGCTGC TGGGGTAGAC GGCCCAGGGC TAGAGTGCGA CCTGGGGCTG 
GTGGAGGATG AGCTGGTGCT GCCAGATGAT GTGGTACAGT ACATCAAGGC TCACACCGGT 
GGTACCTTGG ATGACGGCAT TCGGCAGGGG TATCCCACAG AAGGTACTGG CTTCCCCGAG 
AACTCTAAGC TGCCCAGTCC TGGGCTACAA GGCCACCGCA GGCTAGCAGC TGCCGACTCC 
AACATGGGTC CTTCTGCTCC TGGACTCGGG GGCTGCCAGC TGAGCTACAG CCCCTCCTCC 
AACCTCAACA AGAGCAACAT GCCTGTGCAG TGGAATGAGG TGAGTTCTGG CACCGTGGAT 
GCCCTGCCTA CCCAGGTGAA GCCACCTCCT TTCCCTCACA GCAACCTGGC TGTGGTCCAA 
CAGAAGCCAG CCTTTGGCCA GTATCCAGGA TATAATCCAC AATCCGTGCA GAGCAGCTCC 
GGAGGTCTAG ACAGCACCCA GCCGCACCTA CAGCTTCGAG GAGCCCCCTC TGCATCAAGA 
GGGAGCTACA CGCAACAGCC TCGACAGCCA GCTGCAGGCA GTCAGTGCCT GGGTATGAGT 
GCGGCCATGA GCCCGCAGGC CAGCTACAGC CAAGCCCACC CCCAGCTGAG CCCAAACATT 
GTCAGCGGAT CTCTGAACCA GTTTTCTCCC TCCTGCAGCA ATATGGCAGC CAAGCCCAGC 
CACCTGGGAC TCCCTCAGCA AATGGAAGTT GTCCCCAATG CCACCATCAT GAATGGCCAT 
CAACGGGAGC ACGGGGTCCC CAATTCATCC CTGGCTGCGG TGTCACAACC TCACCCAGTC 
CTGAGCTATC CCCAGCAGGA CAGCTACCAA CAGGGCTCCA ACCTTCTGTC ATCCCATCAG 
CCTGGCTTCA TGGAGTCCCA GCAGAACGCG GGCTTTGGTC TCATGCAGCC TCGGCCACCC 
CTGGAACCCA ACACGGCCAG CCGTCACCGT GGAGTACGTT CTGGGCAACA GCAGTTGTAT 
GCCAGGACCA CTGGCCAAGC CATGGTCACA TCAGCCAACC AAGAGACAGC AGAAGCTATG 
CCCAAGGGAC CAGCAGGGAC CATGGTATCC CTAGCTCCTC AGCCATCTCA GGACACAGGG 
CGGGCACAAG ATCAGAACAC GCTATACTAC TATGGCCAGA TCCACATGTA TGAACAGAAT 
GGAGGCTGCC CAGCCGTGCA GCCCCAGCCG CCACAACCAC AAGCTTGCTC AGACAGTATC 
CAGCCTGAGC CTTTGCCTTC ACCGGGAGTC AACCAGGTGT CTAGCACCGT GGACTCCCAG 
CTCCTGGAGC CCCCCCAGAT TGACTTTGAT GCCATCATGG ATGATGGTGA TCACTCGAGT 
TTGTTTTCTG GTGCACTGAG CCCAACCCTT CTCCACAATC TCTCCCAGAA TTCCTCACGC 
CTCACCACAC CCCGGAATTC CTTGACACTG CCCTCCATCC CTGCGGGCAT CAGCAACATG 
GCCGTGGGCG ACATGAGTTC CATGCTCACC AGCCTGGCTG AAGAGAGCAA GTTTTTAAAC 
ATGATGACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529204.1
CDS	651..5102
Translation	MRHQEGRYHYDPHSVHSVHGPPTLSGSPVISDISLIRLSPHPAGPGESPFSAHHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAHEHLKERGLFSLAAPGTNPSDYYHQMTLMASHPTPYGDLLMQSGGAASAPHLHDYLNPVDASRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMTLTSISTMPTQLSSSSSNCLNDANQNKQNSESAVSSTVNPITIHKRSKVKTEAEGLRPASPLGLTQEQLADLKEDLDRDDCKQEAEVVIYETNCHWADCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHVRAPLLKENGDNEASAEPGGRGPEESVEASSTSHTVEDCLHIKAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGMEMPGTGPGSLGDLTALADTCPGADTSALAAPSTGGLQLRKHMSTVHRFEQLKREKLKSLKDSCSWAGPAPHTRNTKLPPLPVNGSVLENFNNTGGGGPAGLLPSQRLPELTEVTMLSQLQERRDSSTSTMSSAYTVSRRSSGISPYFSSRRSSEASPLGGLRPHNASSADSYDPISTDASRRSSEASQCSGGGPGLLNLTPAQQYNLRAKYAAATGGPPPTPLPGLDRVSLRTRLALLDAPERALPGACPHPLGPRRGSDGPTYSHGHGHGYAGAAPAFPHEGPNSSTRRASDPVRRPDPLILPRVQRFHSTHNMNPGSLPPCADRRGLHVQSHPSVDSNLTRNAYSPRPPSINENVVMEAVAAGVDGPGLECDLGLVEDELVLPDDVVQYIKAHTGGTLDDGIRQGYPTEGTGFPENSKLPSPGLQGHRRLAAADSNMGPSAPGLGGCQLSYSPSSNLNKSNMPVQWNEVSSGTVDALPTQVKPPPFPHSNLAVVQQKPAFGQYPGYNPQSVQSSSGGLDSTQPHLQLRGAPSASRGSYTQQPRQPAAGSQCLGMSAAMSPQASYSQAHPQLSPNIVSGSLNQFSPSCSNMAAKPSHLGLPQQMEVVPNATIMNGHQREHGVPNSSLAAVSQPHPVLSYPQQDSYQQGSNLLSSHQPGFMESQQNAGFGLMQPRPPLEPNTASRHRGVRSGQQQLYARTTGQAMVTSANQETAEAMPKGPAGTMVSLAPQPSQDTGRAQDQNTLYYYGQIHMYEQNGGCPAVQPQPPQPQACSDSIQPEPLPSPGVNQVSSTVDSQLLEPPQIDFDAIMDDGDHSSLFSGALSPTLLHNLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	XM_006529141.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529141.4

ORF Insert Sequence:

ATGAGACACC AGGAGGGAAG GTACCATTAT GACCCTCACT CTGTCCACAG TGTACACGGG 
CCTCCCACCC TAAGTGGCAG CCCTGTCATC TCAGATATCT CCTTGATACG ACTTTCTCCA 
CACCCTGCTG GCCCTGGAGA GTCACCCTTC AGCGCCCACC ACCCCTACGT GAACCCCCAT 
ATGGAGCACT ACCTCCGGTC TGTGCACAGC AGCCCCACAC TCTCAATGAT CTCTGCCGCC 
AGGGGCCTCA GCCCTGCTGA TGTGGCCCAC GAACATCTGA AAGAGAGGGG ACTCTTTAGC 
CTCGCAGCCC CAGGCACCAA CCCTTCAGAC TATTACCACC AGATGACCCT CATGGCAAGC 
CACCCCACCC CTTATGGGGA CCTTCTAATG CAGAGCGGGG GTGCTGCTAG CGCACCCCAT 
CTCCATGACT ACCTCAACCC TGTGGATGCA TCACGATTCT CTAGTCCACG TGTGACCCCA 
CGACTGAGCC GCAAGCGGGC TCTGTCCATC TCCCCGCTCT CAGATGCCAG CCTCGACCTA 
CAACGCATGA TTCGGACCTC TCCCAACTCG CTGGTAGCTT ACATCAACAA CTCCAGGAGC 
AGCTCAGCAG CCAGTGGCTC TTATGGACAT CTGTCTGCTG GTGCCCTCAG CCCAGCCTTC 
ACTTTTCCCC ACCCCATCAA TCCTGTGGCC TACCAGCAGA TCCTGAGCCA GCAGCGGGGC 
CTGGGCTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CTTCACCCAC CTTCTTGGCC 
CAGCAGCCCA TGACTCTCAC CTCCATCAGC ACCATGCCTA CCCAACTCAG CAGCAGTAGC 
AGCAACTGTC TAAATGATGC CAACCAGAAC AAGCAGAACA GCGAGTCAGC TGTGAGCAGC 
ACCGTGAACC CCATCACCAT TCATAAGCGG AGCAAGGTCA AGACTGAGGC TGAGGGCCTG 
CGTCCAGCAT CCCCGCTTGG ACTGACACAG GAGCAGCTGG CCGATCTCAA GGAAGACCTG 
GACAGGGATG ACTGTAAGCA GGAGGCCGAG GTGGTCATCT ACGAGACCAA CTGCCACTGG 
GCAGACTGCA CCAAGGAGTA TGACACACAG GAGCAGCTGG TGCATCATAT CAACAATGAA 
CACATCCACG GGGAGAAGAA GGAGTTCGTG TGCCGCTGGC AGGCCTGCAC GAGAGAGCAG 
AAGCCCTTCA AGGCCCAGTA CATGCTGGTT GTTCACATGC GCAGACACAC GGGTGAGAAG 
CCACACAAGT GCACGTTCGA AGGCTGTTCC AAGGCCTACT CTCGCCTGGA GAACCTGAAG 
ACACACCTGC GTTCACACAC AGGAGAGAAG CCATATGTGT GTGAACACGA AGGCTGTAAC 
AAAGCCTTCT CCAATGCCTC AGACCGCGCC AAGCACCAGA ACCGCACTCA CTCCAATGAG 
AAACCCTACA TCTGCAAGAT CCCAGGCTGC ACCAAGAGGT ACACAGACCC CAGCTCACTC 
CGCAAGCATG TGAAGACTGT CCATGGGCCA GACGCCCATG TCACCAAGAA ACAGCGTAAT 
GATGTGCATG TCCGTGCTCC ACTGCTCAAG GAGAATGGGG ATAATGAGGC CAGCGCCGAG 
CCAGGTGGCC GGGGACCTGA GGAGAGTGTG GAGGCCAGTA GCACCAGCCA CACTGTGGAG 
GACTGCCTAC ATATCAAAGC CATCAAGACA GAGAGCTCCG GGCTTTGTCA GTCCAGCCCC 
GGGGCCCAGT CATCCTGCAG CAGCGAGCCC TCTCCCCTGG GCAGTGCCCC CAACAATGAC 
AGTGGCATGG AGATGCCGGG GACAGGGCCT GGGAGTCTGG GAGACCTGAC AGCACTGGCT 
GACACGTGTC CAGGAGCTGA CACCTCAGCC CTGGCTGCAC CCTCCACTGG TGGCCTGCAG 
CTGCGCAAAC ACATGAGCAC CGTGCATCGC TTTGAGCAGC TGAAGAGAGA GAAGCTCAAG 
TCACTGAAGG ATTCCTGCTC GTGGGCCGGC CCAGCTCCAC ACACCCGCAA CACCAAGCTG 
CCTCCCCTTC CAGTCAATGG TTCTGTCCTG GAAAACTTCA ACAATACAGG GGGCGGTGGA 
CCGGCAGGAC TGCTGCCCAG CCAGCGGCTA CCAGAGCTGA CCGAAGTGAC GATGCTGAGC 
CAGCTGCAGG AACGAAGAGA CAGCTCCACC AGCACCATGA GCTCGGCCTA CACTGTGAGC 
CGCCGCTCCT CTGGCATCTC CCCATACTTC TCTAGCCGTC GCTCCAGCGA GGCTTCGCCT 
CTCGGTGGCC TACGCCCGCA CAACGCCAGC TCAGCAGACT CCTATGACCC CATCTCCACA 
GATGCCTCTC GGCGCTCCAG TGAAGCCAGC CAGTGCAGTG GCGGTGGCCC AGGGCTGCTC 
AACCTCACAC CTGCGCAGCA GTACAACCTG CGTGCCAAGT ACGCAGCGGC CACAGGTGGA 
CCACCGCCCA CGCCACTGCC GGGCCTCGAT CGTGTAAGCC TTCGTACCCG CCTGGCCTTG 
CTGGATGCTC CTGAGCGTGC ACTTCCTGGT GCCTGCCCAC ATCCACTGGG GCCACGGCGT 
GGCAGCGATG GGCCTACCTA TAGCCATGGT CATGGCCATG GCTACGCAGG TGCGGCTCCA 
GCATTCCCCC ACGAGGGGCC AAACAGCAGC ACACGGCGGG CCAGCGACCC TGTGCGGCGC 
CCTGACCCCC TTATTCTGCC TCGAGTGCAA CGTTTCCACA GTACCCACAA CATGAATCCA 
GGTTCACTGC CACCCTGCGC TGATCGGCGT GGCCTGCACG TACAGAGCCA CCCCAGCGTA 
GACAGCAACC TGACCCGCAA CGCCTACTCT CCCAGACCCC CTAGCATCAA TGAGAACGTG 
GTGATGGAGG CCGTGGCTGC TGGGGTAGAC GGCCCAGGGC TAGAGTGCGA CCTGGGGCTG 
GTGGAGGATG AGCTGGTGCT GCCAGATGAT GTGGTACAGT ACATCAAGGC TCACACCGGT 
GGTACCTTGG ATGACGGCAT TCGGCAGGGG TATCCCACAG AAGGTACTGG CTTCCCCGAG 
AACTCTAAGC TGCCCAGTCC TGGGCTACAA GGCCACCGCA GGCTAGCAGC TGCCGACTCC 
AACATGGGTC CTTCTGCTCC TGGACTCGGG GGCTGCCAGC TGAGCTACAG CCCCTCCTCC 
AACCTCAACA AGAGCAACAT GCCTGTGCAG TGGAATGAGG TGAGTTCTGG CACCGTGGAT 
GCCCTGCCTA CCCAGGTGAA GCCACCTCCT TTCCCTCACA GCAACCTGGC TGTGGTCCAA 
CAGAAGCCAG CCTTTGGCCA GTATCCAGGA TATAATCCAC AATCCGTGCA GAGCAGCTCC 
GGAGGTCTAG ACAGCACCCA GCCGCACCTA CAGCTTCGAG GAGCCCCCTC TGCATCAAGA 
GGGAGCTACA CGCAACAGCC TCGACAGCCA GCTGCAGGCA GTCAGTGCCT GGGTATGAGT 
GCGGCCATGA GCCCGCAGGC CAGCTACAGC CAAGCCCACC CCCAGCTGAG CCCAAACATT 
GTCAGCGGAT CTCTGAACCA GTTTTCTCCC TCCTGCAGCA ATATGGCAGC CAAGCCCAGC 
CACCTGGGAC TCCCTCAGCA AATGGAAGTT GTCCCCAATG CCACCATCAT GAATGGCCAT 
CAACGGGAGC ACGGGGTCCC CAATTCATCC CTGGCTGCGG TGTCACAACC TCACCCAGTC 
CTGAGCTATC CCCAGCAGGA CAGCTACCAA CAGGGCTCCA ACCTTCTGTC ATCCCATCAG 
CCTGGCTTCA TGGAGTCCCA GCAGAACGCG GGCTTTGGTC TCATGCAGCC TCGGCCACCC 
CTGGAACCCA ACACGGCCAG CCGTCACCGT GGAGTACGTT CTGGGCAACA GCAGTTGTAT 
GCCAGGACCA CTGGCCAAGC CATGGTCACA TCAGCCAACC AAGAGACAGC AGAAGCTATG 
CCCAAGGGAC CAGCAGGGAC CATGGTATCC CTAGCTCCTC AGCCATCTCA GGACACAGGG 
CGGGCACAAG ATCAGAACAC GCTATACTAC TATGGCCAGA TCCACATGTA TGAACAGAAT 
GGAGGCTGCC CAGCCGTGCA GCCCCAGCCG CCACAACCAC AAGCTTGCTC AGACAGTATC 
CAGCCTGAGC CTTTGCCTTC ACCGGGAGTC AACCAGGTGT CTAGCACCGT GGACTCCCAG 
CTCCTGGAGC CCCCCCAGAT TGACTTTGAT GCCATCATGG ATGATGGTGA TCACTCGAGT 
TTGTTTTCTG GTGCACTGAG CCCAACCCTT CTCCACAATC TCTCCCAGAA TTCCTCACGC 
CTCACCACAC CCCGGAATTC CTTGACACTG CCCTCCATCC CTGCGGGCAT CAGCAACATG 
GCCGTGGGCG ACATGAGTTC CATGCTCACC AGCCTGGCTG AAGAGAGCAA GTTTTTAAAC 
ATGATGACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gli2 ( XM_011247924.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246226.1 Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu27904
Clone ID Related Accession (Same CDS sequence)	XM_011247924.2 , XM_006529141.3 , XM_006529141.4 , XM_011247924.3
Accession Version	XM_011247924.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	zinc finger protein GLI2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247924.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACACC AGGAGGGAAG GTACCATTAT GACCCTCACT CTGTCCACAG TGTACACGGG 
CCTCCCACCC TAAGTGGCAG CCCTGTCATC TCAGATATCT CCTTGATACG ACTTTCTCCA 
CACCCTGCTG GCCCTGGAGA GTCACCCTTC AGCGCCCACC ACCCCTACGT GAACCCCCAT 
ATGGAGCACT ACCTCCGGTC TGTGCACAGC AGCCCCACAC TCTCAATGAT CTCTGCCGCC 
AGGGGCCTCA GCCCTGCTGA TGTGGCCCAC GAACATCTGA AAGAGAGGGG ACTCTTTAGC 
CTCGCAGCCC CAGGCACCAA CCCTTCAGAC TATTACCACC AGATGACCCT CATGGCAAGC 
CACCCCACCC CTTATGGGGA CCTTCTAATG CAGAGCGGGG GTGCTGCTAG CGCACCCCAT 
CTCCATGACT ACCTCAACCC TGTGGATGCA TCACGATTCT CTAGTCCACG TGTGACCCCA 
CGACTGAGCC GCAAGCGGGC TCTGTCCATC TCCCCGCTCT CAGATGCCAG CCTCGACCTA 
CAACGCATGA TTCGGACCTC TCCCAACTCG CTGGTAGCTT ACATCAACAA CTCCAGGAGC 
AGCTCAGCAG CCAGTGGCTC TTATGGACAT CTGTCTGCTG GTGCCCTCAG CCCAGCCTTC 
ACTTTTCCCC ACCCCATCAA TCCTGTGGCC TACCAGCAGA TCCTGAGCCA GCAGCGGGGC 
CTGGGCTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CTTCACCCAC CTTCTTGGCC 
CAGCAGCCCA TGACTCTCAC CTCCATCAGC ACCATGCCTA CCCAACTCAG CAGCAGTAGC 
AGCAACTGTC TAAATGATGC CAACCAGAAC AAGCAGAACA GCGAGTCAGC TGTGAGCAGC 
ACCGTGAACC CCATCACCAT TCATAAGCGG AGCAAGGTCA AGACTGAGGC TGAGGGCCTG 
CGTCCAGCAT CCCCGCTTGG ACTGACACAG GAGCAGCTGG CCGATCTCAA GGAAGACCTG 
GACAGGGATG ACTGTAAGCA GGAGGCCGAG GTGGTCATCT ACGAGACCAA CTGCCACTGG 
GCAGACTGCA CCAAGGAGTA TGACACACAG GAGCAGCTGG TGCATCATAT CAACAATGAA 
CACATCCACG GGGAGAAGAA GGAGTTCGTG TGCCGCTGGC AGGCCTGCAC GAGAGAGCAG 
AAGCCCTTCA AGGCCCAGTA CATGCTGGTT GTTCACATGC GCAGACACAC GGGTGAGAAG 
CCACACAAGT GCACGTTCGA AGGCTGTTCC AAGGCCTACT CTCGCCTGGA GAACCTGAAG 
ACACACCTGC GTTCACACAC AGGAGAGAAG CCATATGTGT GTGAACACGA AGGCTGTAAC 
AAAGCCTTCT CCAATGCCTC AGACCGCGCC AAGCACCAGA ACCGCACTCA CTCCAATGAG 
AAACCCTACA TCTGCAAGAT CCCAGGCTGC ACCAAGAGGT ACACAGACCC CAGCTCACTC 
CGCAAGCATG TGAAGACTGT CCATGGGCCA GACGCCCATG TCACCAAGAA ACAGCGTAAT 
GATGTGCATG TCCGTGCTCC ACTGCTCAAG GAGAATGGGG ATAATGAGGC CAGCGCCGAG 
CCAGGTGGCC GGGGACCTGA GGAGAGTGTG GAGGCCAGTA GCACCAGCCA CACTGTGGAG 
GACTGCCTAC ATATCAAAGC CATCAAGACA GAGAGCTCCG GGCTTTGTCA GTCCAGCCCC 
GGGGCCCAGT CATCCTGCAG CAGCGAGCCC TCTCCCCTGG GCAGTGCCCC CAACAATGAC 
AGTGGCATGG AGATGCCGGG GACAGGGCCT GGGAGTCTGG GAGACCTGAC AGCACTGGCT 
GACACGTGTC CAGGAGCTGA CACCTCAGCC CTGGCTGCAC CCTCCACTGG TGGCCTGCAG 
CTGCGCAAAC ACATGAGCAC CGTGCATCGC TTTGAGCAGC TGAAGAGAGA GAAGCTCAAG 
TCACTGAAGG ATTCCTGCTC GTGGGCCGGC CCAGCTCCAC ACACCCGCAA CACCAAGCTG 
CCTCCCCTTC CAGTCAATGG TTCTGTCCTG GAAAACTTCA ACAATACAGG GGGCGGTGGA 
CCGGCAGGAC TGCTGCCCAG CCAGCGGCTA CCAGAGCTGA CCGAAGTGAC GATGCTGAGC 
CAGCTGCAGG AACGAAGAGA CAGCTCCACC AGCACCATGA GCTCGGCCTA CACTGTGAGC 
CGCCGCTCCT CTGGCATCTC CCCATACTTC TCTAGCCGTC GCTCCAGCGA GGCTTCGCCT 
CTCGGTGGCC TACGCCCGCA CAACGCCAGC TCAGCAGACT CCTATGACCC CATCTCCACA 
GATGCCTCTC GGCGCTCCAG TGAAGCCAGC CAGTGCAGTG GCGGTGGCCC AGGGCTGCTC 
AACCTCACAC CTGCGCAGCA GTACAACCTG CGTGCCAAGT ACGCAGCGGC CACAGGTGGA 
CCACCGCCCA CGCCACTGCC GGGCCTCGAT CGTGTAAGCC TTCGTACCCG CCTGGCCTTG 
CTGGATGCTC CTGAGCGTGC ACTTCCTGGT GCCTGCCCAC ATCCACTGGG GCCACGGCGT 
GGCAGCGATG GGCCTACCTA TAGCCATGGT CATGGCCATG GCTACGCAGG TGCGGCTCCA 
GCATTCCCCC ACGAGGGGCC AAACAGCAGC ACACGGCGGG CCAGCGACCC TGTGCGGCGC 
CCTGACCCCC TTATTCTGCC TCGAGTGCAA CGTTTCCACA GTACCCACAA CATGAATCCA 
GGTTCACTGC CACCCTGCGC TGATCGGCGT GGCCTGCACG TACAGAGCCA CCCCAGCGTA 
GACAGCAACC TGACCCGCAA CGCCTACTCT CCCAGACCCC CTAGCATCAA TGAGAACGTG 
GTGATGGAGG CCGTGGCTGC TGGGGTAGAC GGCCCAGGGC TAGAGTGCGA CCTGGGGCTG 
GTGGAGGATG AGCTGGTGCT GCCAGATGAT GTGGTACAGT ACATCAAGGC TCACACCGGT 
GGTACCTTGG ATGACGGCAT TCGGCAGGGG TATCCCACAG AAGGTACTGG CTTCCCCGAG 
AACTCTAAGC TGCCCAGTCC TGGGCTACAA GGCCACCGCA GGCTAGCAGC TGCCGACTCC 
AACATGGGTC CTTCTGCTCC TGGACTCGGG GGCTGCCAGC TGAGCTACAG CCCCTCCTCC 
AACCTCAACA AGAGCAACAT GCCTGTGCAG TGGAATGAGG TGAGTTCTGG CACCGTGGAT 
GCCCTGCCTA CCCAGGTGAA GCCACCTCCT TTCCCTCACA GCAACCTGGC TGTGGTCCAA 
CAGAAGCCAG CCTTTGGCCA GTATCCAGGA TATAATCCAC AATCCGTGCA GAGCAGCTCC 
GGAGGTCTAG ACAGCACCCA GCCGCACCTA CAGCTTCGAG GAGCCCCCTC TGCATCAAGA 
GGGAGCTACA CGCAACAGCC TCGACAGCCA GCTGCAGGCA GTCAGTGCCT GGGTATGAGT 
GCGGCCATGA GCCCGCAGGC CAGCTACAGC CAAGCCCACC CCCAGCTGAG CCCAAACATT 
GTCAGCGGAT CTCTGAACCA GTTTTCTCCC TCCTGCAGCA ATATGGCAGC CAAGCCCAGC 
CACCTGGGAC TCCCTCAGCA AATGGAAGTT GTCCCCAATG CCACCATCAT GAATGGCCAT 
CAACGGGAGC ACGGGGTCCC CAATTCATCC CTGGCTGCGG TGTCACAACC TCACCCAGTC 
CTGAGCTATC CCCAGCAGGA CAGCTACCAA CAGGGCTCCA ACCTTCTGTC ATCCCATCAG 
CCTGGCTTCA TGGAGTCCCA GCAGAACGCG GGCTTTGGTC TCATGCAGCC TCGGCCACCC 
CTGGAACCCA ACACGGCCAG CCGTCACCGT GGAGTACGTT CTGGGCAACA GCAGTTGTAT 
GCCAGGACCA CTGGCCAAGC CATGGTCACA TCAGCCAACC AAGAGACAGC AGAAGCTATG 
CCCAAGGGAC CAGCAGGGAC CATGGTATCC CTAGCTCCTC AGCCATCTCA GGACACAGGG 
CGGGCACAAG ATCAGAACAC GCTATACTAC TATGGCCAGA TCCACATGTA TGAACAGAAT 
GGAGGCTGCC CAGCCGTGCA GCCCCAGCCG CCACAACCAC AAGCTTGCTC AGACAGTATC 
CAGCCTGAGC CTTTGCCTTC ACCGGGAGTC AACCAGGTGT CTAGCACCGT GGACTCCCAG 
CTCCTGGAGC CCCCCCAGAT TGACTTTGAT GCCATCATGG ATGATGGTGA TCACTCGAGT 
TTGTTTTCTG GTGCACTGAG CCCAACCCTT CTCCACAATC TCTCCCAGAA TTCCTCACGC 
CTCACCACAC CCCGGAATTC CTTGACACTG CCCTCCATCC CTGCGGGCAT CAGCAACATG 
GCCGTGGGCG ACATGAGTTC CATGCTCACC AGCCTGGCTG AAGAGAGCAA GTTTTTAAAC 
ATGATGACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246226.1
CDS	352..4803
Translation	MRHQEGRYHYDPHSVHSVHGPPTLSGSPVISDISLIRLSPHPAGPGESPFSAHHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAHEHLKERGLFSLAAPGTNPSDYYHQMTLMASHPTPYGDLLMQSGGAASAPHLHDYLNPVDASRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMTLTSISTMPTQLSSSSSNCLNDANQNKQNSESAVSSTVNPITIHKRSKVKTEAEGLRPASPLGLTQEQLADLKEDLDRDDCKQEAEVVIYETNCHWADCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHVRAPLLKENGDNEASAEPGGRGPEESVEASSTSHTVEDCLHIKAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGMEMPGTGPGSLGDLTALADTCPGADTSALAAPSTGGLQLRKHMSTVHRFEQLKREKLKSLKDSCSWAGPAPHTRNTKLPPLPVNGSVLENFNNTGGGGPAGLLPSQRLPELTEVTMLSQLQERRDSSTSTMSSAYTVSRRSSGISPYFSSRRSSEASPLGGLRPHNASSADSYDPISTDASRRSSEASQCSGGGPGLLNLTPAQQYNLRAKYAAATGGPPPTPLPGLDRVSLRTRLALLDAPERALPGACPHPLGPRRGSDGPTYSHGHGHGYAGAAPAFPHEGPNSSTRRASDPVRRPDPLILPRVQRFHSTHNMNPGSLPPCADRRGLHVQSHPSVDSNLTRNAYSPRPPSINENVVMEAVAAGVDGPGLECDLGLVEDELVLPDDVVQYIKAHTGGTLDDGIRQGYPTEGTGFPENSKLPSPGLQGHRRLAAADSNMGPSAPGLGGCQLSYSPSSNLNKSNMPVQWNEVSSGTVDALPTQVKPPPFPHSNLAVVQQKPAFGQYPGYNPQSVQSSSGGLDSTQPHLQLRGAPSASRGSYTQQPRQPAAGSQCLGMSAAMSPQASYSQAHPQLSPNIVSGSLNQFSPSCSNMAAKPSHLGLPQQMEVVPNATIMNGHQREHGVPNSSLAAVSQPHPVLSYPQQDSYQQGSNLLSSHQPGFMESQQNAGFGLMQPRPPLEPNTASRHRGVRSGQQQLYARTTGQAMVTSANQETAEAMPKGPAGTMVSLAPQPSQDTGRAQDQNTLYYYGQIHMYEQNGGCPAVQPQPPQPQACSDSIQPEPLPSPGVNQVSSTVDSQLLEPPQIDFDAIMDDGDHSSLFSGALSPTLLHNLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	XM_011247924.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247924.3

ORF Insert Sequence:

ATGAGACACC AGGAGGGAAG GTACCATTAT GACCCTCACT CTGTCCACAG TGTACACGGG 
CCTCCCACCC TAAGTGGCAG CCCTGTCATC TCAGATATCT CCTTGATACG ACTTTCTCCA 
CACCCTGCTG GCCCTGGAGA GTCACCCTTC AGCGCCCACC ACCCCTACGT GAACCCCCAT 
ATGGAGCACT ACCTCCGGTC TGTGCACAGC AGCCCCACAC TCTCAATGAT CTCTGCCGCC 
AGGGGCCTCA GCCCTGCTGA TGTGGCCCAC GAACATCTGA AAGAGAGGGG ACTCTTTAGC 
CTCGCAGCCC CAGGCACCAA CCCTTCAGAC TATTACCACC AGATGACCCT CATGGCAAGC 
CACCCCACCC CTTATGGGGA CCTTCTAATG CAGAGCGGGG GTGCTGCTAG CGCACCCCAT 
CTCCATGACT ACCTCAACCC TGTGGATGCA TCACGATTCT CTAGTCCACG TGTGACCCCA 
CGACTGAGCC GCAAGCGGGC TCTGTCCATC TCCCCGCTCT CAGATGCCAG CCTCGACCTA 
CAACGCATGA TTCGGACCTC TCCCAACTCG CTGGTAGCTT ACATCAACAA CTCCAGGAGC 
AGCTCAGCAG CCAGTGGCTC TTATGGACAT CTGTCTGCTG GTGCCCTCAG CCCAGCCTTC 
ACTTTTCCCC ACCCCATCAA TCCTGTGGCC TACCAGCAGA TCCTGAGCCA GCAGCGGGGC 
CTGGGCTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CTTCACCCAC CTTCTTGGCC 
CAGCAGCCCA TGACTCTCAC CTCCATCAGC ACCATGCCTA CCCAACTCAG CAGCAGTAGC 
AGCAACTGTC TAAATGATGC CAACCAGAAC AAGCAGAACA GCGAGTCAGC TGTGAGCAGC 
ACCGTGAACC CCATCACCAT TCATAAGCGG AGCAAGGTCA AGACTGAGGC TGAGGGCCTG 
CGTCCAGCAT CCCCGCTTGG ACTGACACAG GAGCAGCTGG CCGATCTCAA GGAAGACCTG 
GACAGGGATG ACTGTAAGCA GGAGGCCGAG GTGGTCATCT ACGAGACCAA CTGCCACTGG 
GCAGACTGCA CCAAGGAGTA TGACACACAG GAGCAGCTGG TGCATCATAT CAACAATGAA 
CACATCCACG GGGAGAAGAA GGAGTTCGTG TGCCGCTGGC AGGCCTGCAC GAGAGAGCAG 
AAGCCCTTCA AGGCCCAGTA CATGCTGGTT GTTCACATGC GCAGACACAC GGGTGAGAAG 
CCACACAAGT GCACGTTCGA AGGCTGTTCC AAGGCCTACT CTCGCCTGGA GAACCTGAAG 
ACACACCTGC GTTCACACAC AGGAGAGAAG CCATATGTGT GTGAACACGA AGGCTGTAAC 
AAAGCCTTCT CCAATGCCTC AGACCGCGCC AAGCACCAGA ACCGCACTCA CTCCAATGAG 
AAACCCTACA TCTGCAAGAT CCCAGGCTGC ACCAAGAGGT ACACAGACCC CAGCTCACTC 
CGCAAGCATG TGAAGACTGT CCATGGGCCA GACGCCCATG TCACCAAGAA ACAGCGTAAT 
GATGTGCATG TCCGTGCTCC ACTGCTCAAG GAGAATGGGG ATAATGAGGC CAGCGCCGAG 
CCAGGTGGCC GGGGACCTGA GGAGAGTGTG GAGGCCAGTA GCACCAGCCA CACTGTGGAG 
GACTGCCTAC ATATCAAAGC CATCAAGACA GAGAGCTCCG GGCTTTGTCA GTCCAGCCCC 
GGGGCCCAGT CATCCTGCAG CAGCGAGCCC TCTCCCCTGG GCAGTGCCCC CAACAATGAC 
AGTGGCATGG AGATGCCGGG GACAGGGCCT GGGAGTCTGG GAGACCTGAC AGCACTGGCT 
GACACGTGTC CAGGAGCTGA CACCTCAGCC CTGGCTGCAC CCTCCACTGG TGGCCTGCAG 
CTGCGCAAAC ACATGAGCAC CGTGCATCGC TTTGAGCAGC TGAAGAGAGA GAAGCTCAAG 
TCACTGAAGG ATTCCTGCTC GTGGGCCGGC CCAGCTCCAC ACACCCGCAA CACCAAGCTG 
CCTCCCCTTC CAGTCAATGG TTCTGTCCTG GAAAACTTCA ACAATACAGG GGGCGGTGGA 
CCGGCAGGAC TGCTGCCCAG CCAGCGGCTA CCAGAGCTGA CCGAAGTGAC GATGCTGAGC 
CAGCTGCAGG AACGAAGAGA CAGCTCCACC AGCACCATGA GCTCGGCCTA CACTGTGAGC 
CGCCGCTCCT CTGGCATCTC CCCATACTTC TCTAGCCGTC GCTCCAGCGA GGCTTCGCCT 
CTCGGTGGCC TACGCCCGCA CAACGCCAGC TCAGCAGACT CCTATGACCC CATCTCCACA 
GATGCCTCTC GGCGCTCCAG TGAAGCCAGC CAGTGCAGTG GCGGTGGCCC AGGGCTGCTC 
AACCTCACAC CTGCGCAGCA GTACAACCTG CGTGCCAAGT ACGCAGCGGC CACAGGTGGA 
CCACCGCCCA CGCCACTGCC GGGCCTCGAT CGTGTAAGCC TTCGTACCCG CCTGGCCTTG 
CTGGATGCTC CTGAGCGTGC ACTTCCTGGT GCCTGCCCAC ATCCACTGGG GCCACGGCGT 
GGCAGCGATG GGCCTACCTA TAGCCATGGT CATGGCCATG GCTACGCAGG TGCGGCTCCA 
GCATTCCCCC ACGAGGGGCC AAACAGCAGC ACACGGCGGG CCAGCGACCC TGTGCGGCGC 
CCTGACCCCC TTATTCTGCC TCGAGTGCAA CGTTTCCACA GTACCCACAA CATGAATCCA 
GGTTCACTGC CACCCTGCGC TGATCGGCGT GGCCTGCACG TACAGAGCCA CCCCAGCGTA 
GACAGCAACC TGACCCGCAA CGCCTACTCT CCCAGACCCC CTAGCATCAA TGAGAACGTG 
GTGATGGAGG CCGTGGCTGC TGGGGTAGAC GGCCCAGGGC TAGAGTGCGA CCTGGGGCTG 
GTGGAGGATG AGCTGGTGCT GCCAGATGAT GTGGTACAGT ACATCAAGGC TCACACCGGT 
GGTACCTTGG ATGACGGCAT TCGGCAGGGG TATCCCACAG AAGGTACTGG CTTCCCCGAG 
AACTCTAAGC TGCCCAGTCC TGGGCTACAA GGCCACCGCA GGCTAGCAGC TGCCGACTCC 
AACATGGGTC CTTCTGCTCC TGGACTCGGG GGCTGCCAGC TGAGCTACAG CCCCTCCTCC 
AACCTCAACA AGAGCAACAT GCCTGTGCAG TGGAATGAGG TGAGTTCTGG CACCGTGGAT 
GCCCTGCCTA CCCAGGTGAA GCCACCTCCT TTCCCTCACA GCAACCTGGC TGTGGTCCAA 
CAGAAGCCAG CCTTTGGCCA GTATCCAGGA TATAATCCAC AATCCGTGCA GAGCAGCTCC 
GGAGGTCTAG ACAGCACCCA GCCGCACCTA CAGCTTCGAG GAGCCCCCTC TGCATCAAGA 
GGGAGCTACA CGCAACAGCC TCGACAGCCA GCTGCAGGCA GTCAGTGCCT GGGTATGAGT 
GCGGCCATGA GCCCGCAGGC CAGCTACAGC CAAGCCCACC CCCAGCTGAG CCCAAACATT 
GTCAGCGGAT CTCTGAACCA GTTTTCTCCC TCCTGCAGCA ATATGGCAGC CAAGCCCAGC 
CACCTGGGAC TCCCTCAGCA AATGGAAGTT GTCCCCAATG CCACCATCAT GAATGGCCAT 
CAACGGGAGC ACGGGGTCCC CAATTCATCC CTGGCTGCGG TGTCACAACC TCACCCAGTC 
CTGAGCTATC CCCAGCAGGA CAGCTACCAA CAGGGCTCCA ACCTTCTGTC ATCCCATCAG 
CCTGGCTTCA TGGAGTCCCA GCAGAACGCG GGCTTTGGTC TCATGCAGCC TCGGCCACCC 
CTGGAACCCA ACACGGCCAG CCGTCACCGT GGAGTACGTT CTGGGCAACA GCAGTTGTAT 
GCCAGGACCA CTGGCCAAGC CATGGTCACA TCAGCCAACC AAGAGACAGC AGAAGCTATG 
CCCAAGGGAC CAGCAGGGAC CATGGTATCC CTAGCTCCTC AGCCATCTCA GGACACAGGG 
CGGGCACAAG ATCAGAACAC GCTATACTAC TATGGCCAGA TCCACATGTA TGAACAGAAT 
GGAGGCTGCC CAGCCGTGCA GCCCCAGCCG CCACAACCAC AAGCTTGCTC AGACAGTATC 
CAGCCTGAGC CTTTGCCTTC ACCGGGAGTC AACCAGGTGT CTAGCACCGT GGACTCCCAG 
CTCCTGGAGC CCCCCCAGAT TGACTTTGAT GCCATCATGG ATGATGGTGA TCACTCGAGT 
TTGTTTTCTG GTGCACTGAG CCCAACCCTT CTCCACAATC TCTCCCAGAA TTCCTCACGC 
CTCACCACAC CCCGGAATTC CTTGACACTG CCCTCCATCC CTGCGGGCAT CAGCAACATG 
GCCGTGGGCG ACATGAGTTC CATGCTCACC AGCCTGGCTG AAGAGAGCAA GTTTTTAAAC 
ATGATGACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gli2 ( XM_030248278.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104138.1 Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X1, mRNA.

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CloneID	OMu22271
Clone ID Related Accession (Same CDS sequence)	XM_030248278.1 , NM_001081125.1
Accession Version	XM_030248278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	zinc finger protein GLI2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTT CTGCCCCAGC CCCTGCACTG GAGAAGAAAG AAGCCAAGAG TGGTCTCTTG 
GAGGACAGCA GCTTCCCCGA CCCAGGGAAA AAGGCCTGTC CTCTGGCGGT GGCCGCAGCT 
GTAGCCGCCC ACGGAGTTCC TCAGCAGCTC CTGCCGGCTT TCCACGCGCC TTTGCCGATT 
GACATGAGAC ACCAGGAGGG AAGGTACCAT TATGACCCTC ACTCTGTCCA CAGTGTACAC 
GGGCCTCCCA CCCTAAGTGG CAGCCCTGTC ATCTCAGATA TCTCCTTGAT ACGACTTTCT 
CCACACCCTG CTGGCCCTGG AGAGTCACCC TTCAGCGCCC ACCACCCCTA CGTGAACCCC 
CATATGGAGC ACTACCTCCG GTCTGTGCAC AGCAGCCCCA CACTCTCAAT GATCTCTGCC 
GCCAGGGGCC TCAGCCCTGC TGATGTGGCC CACGAACATC TGAAAGAGAG GGGACTCTTT 
AGCCTCGCAG CCCCAGGCAC CAACCCTTCA GACTATTACC ACCAGATGAC CCTCATGGCA 
AGCCACCCCA CCCCTTATGG GGACCTTCTA ATGCAGAGCG GGGGTGCTGC TAGCGCACCC 
CATCTCCATG ACTACCTCAA CCCTGTGGAT GCATCACGAT TCTCTAGTCC ACGTGTGACC 
CCACGACTGA GCCGCAAGCG GGCTCTGTCC ATCTCCCCGC TCTCAGATGC CAGCCTCGAC 
CTACAACGCA TGATTCGGAC CTCTCCCAAC TCGCTGGTAG CTTACATCAA CAACTCCAGG 
AGCAGCTCAG CAGCCAGTGG CTCTTATGGA CATCTGTCTG CTGGTGCCCT CAGCCCAGCC 
TTCACTTTTC CCCACCCCAT CAATCCTGTG GCCTACCAGC AGATCCTGAG CCAGCAGCGG 
GGCCTGGGCT CAGCCTTTGG ACACACACCA CCCCTGATCC AGCCTTCACC CACCTTCTTG 
GCCCAGCAGC CCATGACTCT CACCTCCATC AGCACCATGC CTACCCAACT CAGCAGCAGT 
AGCAGCAACT GTCTAAATGA TGCCAACCAG AACAAGCAGA ACAGCGAGTC AGCTGTGAGC 
AGCACCGTGA ACCCCATCAC CATTCATAAG CGGAGCAAGG TCAAGACTGA GGCTGAGGGC 
CTGCGTCCAG CATCCCCGCT TGGACTGACA CAGGAGCAGC TGGCCGATCT CAAGGAAGAC 
CTGGACAGGG ATGACTGTAA GCAGGAGGCC GAGGTGGTCA TCTACGAGAC CAACTGCCAC 
TGGGCAGACT GCACCAAGGA GTATGACACA CAGGAGCAGC TGGTGCATCA TATCAACAAT 
GAACACATCC ACGGGGAGAA GAAGGAGTTC GTGTGCCGCT GGCAGGCCTG CACGAGAGAG 
CAGAAGCCCT TCAAGGCCCA GTACATGCTG GTTGTTCACA TGCGCAGACA CACGGGTGAG 
AAGCCACACA AGTGCACGTT CGAAGGCTGT TCCAAGGCCT ACTCTCGCCT GGAGAACCTG 
AAGACACACC TGCGTTCACA CACAGGAGAG AAGCCATATG TGTGTGAACA CGAAGGCTGT 
AACAAAGCCT TCTCCAATGC CTCAGACCGC GCCAAGCACC AGAACCGCAC TCACTCCAAT 
GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GGTACACAGA CCCCAGCTCA 
CTCCGCAAGC ATGTGAAGAC TGTCCATGGG CCAGACGCCC ATGTCACCAA GAAACAGCGT 
AATGATGTGC ATGTCCGTGC TCCACTGCTC AAGGAGAATG GGGATAATGA GGCCAGCGCC 
GAGCCAGGTG GCCGGGGACC TGAGGAGAGT GTGGAGGCCA GTAGCACCAG CCACACTGTG 
GAGGACTGCC TACATATCAA AGCCATCAAG ACAGAGAGCT CCGGGCTTTG TCAGTCCAGC 
CCCGGGGCCC AGTCATCCTG CAGCAGCGAG CCCTCTCCCC TGGGCAGTGC CCCCAACAAT 
GACAGTGGCA TGGAGATGCC GGGGACAGGG CCTGGGAGTC TGGGAGACCT GACAGCACTG 
GCTGACACGT GTCCAGGAGC TGACACCTCA GCCCTGGCTG CACCCTCCAC TGGTGGCCTG 
CAGCTGCGCA AACACATGAG CACCGTGCAT CGCTTTGAGC AGCTGAAGAG AGAGAAGCTC 
AAGTCACTGA AGGATTCCTG CTCGTGGGCC GGCCCAGCTC CACACACCCG CAACACCAAG 
CTGCCTCCCC TTCCAGTCAA TGGTTCTGTC CTGGAAAACT TCAACAATAC AGGGGGCGGT 
GGACCGGCAG GACTGCTGCC CAGCCAGCGG CTACCAGAGC TGACCGAAGT GACGATGCTG 
AGCCAGCTGC AGGAACGAAG AGACAGCTCC ACCAGCACCA TGAGCTCGGC CTACACTGTG 
AGCCGCCGCT CCTCTGGCAT CTCCCCATAC TTCTCTAGCC GTCGCTCCAG CGAGGCTTCG 
CCTCTCGGTG GCCTACGCCC GCACAACGCC AGCTCAGCAG ACTCCTATGA CCCCATCTCC 
ACAGATGCCT CTCGGCGCTC CAGTGAAGCC AGCCAGTGCA GTGGCGGTGG CCCAGGGCTG 
CTCAACCTCA CACCTGCGCA GCAGTACAAC CTGCGTGCCA AGTACGCAGC GGCCACAGGT 
GGACCACCGC CCACGCCACT GCCGGGCCTC GATCGTGTAA GCCTTCGTAC CCGCCTGGCC 
TTGCTGGATG CTCCTGAGCG TGCACTTCCT GGTGCCTGCC CACATCCACT GGGGCCACGG 
CGTGGCAGCG ATGGGCCTAC CTATAGCCAT GGTCATGGCC ATGGCTACGC AGGTGCGGCT 
CCAGCATTCC CCCACGAGGG GCCAAACAGC AGCACACGGC GGGCCAGCGA CCCTGTGCGG 
CGCCCTGACC CCCTTATTCT GCCTCGAGTG CAACGTTTCC ACAGTACCCA CAACATGAAT 
CCAGGTTCAC TGCCACCCTG CGCTGATCGG CGTGGCCTGC ACGTACAGAG CCACCCCAGC 
GTAGACAGCA ACCTGACCCG CAACGCCTAC TCTCCCAGAC CCCCTAGCAT CAATGAGAAC 
GTGGTGATGG AGGCCGTGGC TGCTGGGGTA GACGGCCCAG GGCTAGAGTG CGACCTGGGG 
CTGGTGGAGG ATGAGCTGGT GCTGCCAGAT GATGTGGTAC AGTACATCAA GGCTCACACC 
GGTGGTACCT TGGATGACGG CATTCGGCAG GGGTATCCCA CAGAAGGTAC TGGCTTCCCC 
GAGAACTCTA AGCTGCCCAG TCCTGGGCTA CAAGGCCACC GCAGGCTAGC AGCTGCCGAC 
TCCAACATGG GTCCTTCTGC TCCTGGACTC GGGGGCTGCC AGCTGAGCTA CAGCCCCTCC 
TCCAACCTCA ACAAGAGCAA CATGCCTGTG CAGTGGAATG AGGTGAGTTC TGGCACCGTG 
GATGCCCTGC CTACCCAGGT GAAGCCACCT CCTTTCCCTC ACAGCAACCT GGCTGTGGTC 
CAACAGAAGC CAGCCTTTGG CCAGTATCCA GGATATAATC CACAATCCGT GCAGAGCAGC 
TCCGGAGGTC TAGACAGCAC CCAGCCGCAC CTACAGCTTC GAGGAGCCCC CTCTGCATCA 
AGAGGGAGCT ACACGCAACA GCCTCGACAG CCAGCTGCAG GCAGTCAGTG CCTGGGTATG 
AGTGCGGCCA TGAGCCCGCA GGCCAGCTAC AGCCAAGCCC ACCCCCAGCT GAGCCCAAAC 
ATTGTCAGCG GATCTCTGAA CCAGTTTTCT CCCTCCTGCA GCAATATGGC AGCCAAGCCC 
AGCCACCTGG GACTCCCTCA GCAAATGGAA GTTGTCCCCA ATGCCACCAT CATGAATGGC 
CATCAACGGG AGCACGGGGT CCCCAATTCA TCCCTGGCTG CGGTGTCACA ACCTCACCCA 
GTCCTGAGCT ATCCCCAGCA GGACAGCTAC CAACAGGGCT CCAACCTTCT GTCATCCCAT 
CAGCCTGGCT TCATGGAGTC CCAGCAGAAC GCGGGCTTTG GTCTCATGCA GCCTCGGCCA 
CCCCTGGAAC CCAACACGGC CAGCCGTCAC CGTGGAGTAC GTTCTGGGCA ACAGCAGTTG 
TATGCCAGGA CCACTGGCCA AGCCATGGTC ACATCAGCCA ACCAAGAGAC AGCAGAAGCT 
ATGCCCAAGG GACCAGCAGG GACCATGGTA TCCCTAGCTC CTCAGCCATC TCAGGACACA 
GGGCGGGCAC AAGATCAGAA CACGCTATAC TACTATGGCC AGATCCACAT GTATGAACAG 
AATGGAGGCT GCCCAGCCGT GCAGCCCCAG CCGCCACAAC CACAAGCTTG CTCAGACAGT 
ATCCAGCCTG AGCCTTTGCC TTCACCGGGA GTCAACCAGG TGTCTAGCAC CGTGGACTCC 
CAGCTCCTGG AGCCCCCCCA GATTGACTTT GATGCCATCA TGGATGATGG TGATCACTCG 
AGTTTGTTTT CTGGTGCACT GAGCCCAACC CTTCTCCACA ATCTCTCCCA GAATTCCTCA 
CGCCTCACCA CACCCCGGAA TTCCTTGACA CTGCCCTCCA TCCCTGCGGG CATCAGCAAC 
ATGGCCGTGG GCGACATGAG TTCCATGCTC ACCAGCCTGG CTGAAGAGAG CAAGTTTTTA 
AACATGATGA CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104138.1
CDS	398..5032
Translation	METSAPAPALEKKEAKSGLLEDSSFPDPGKKACPLAVAAAVAAHGVPQQLLPAFHAPLPIDMRHQEGRYHYDPHSVHSVHGPPTLSGSPVISDISLIRLSPHPAGPGESPFSAHHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAHEHLKERGLFSLAAPGTNPSDYYHQMTLMASHPTPYGDLLMQSGGAASAPHLHDYLNPVDASRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMTLTSISTMPTQLSSSSSNCLNDANQNKQNSESAVSSTVNPITIHKRSKVKTEAEGLRPASPLGLTQEQLADLKEDLDRDDCKQEAEVVIYETNCHWADCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHVRAPLLKENGDNEASAEPGGRGPEESVEASSTSHTVEDCLHIKAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGMEMPGTGPGSLGDLTALADTCPGADTSALAAPSTGGLQLRKHMSTVHRFEQLKREKLKSLKDSCSWAGPAPHTRNTKLPPLPVNGSVLENFNNTGGGGPAGLLPSQRLPELTEVTMLSQLQERRDSSTSTMSSAYTVSRRSSGISPYFSSRRSSEASPLGGLRPHNASSADSYDPISTDASRRSSEASQCSGGGPGLLNLTPAQQYNLRAKYAAATGGPPPTPLPGLDRVSLRTRLALLDAPERALPGACPHPLGPRRGSDGPTYSHGHGHGYAGAAPAFPHEGPNSSTRRASDPVRRPDPLILPRVQRFHSTHNMNPGSLPPCADRRGLHVQSHPSVDSNLTRNAYSPRPPSINENVVMEAVAAGVDGPGLECDLGLVEDELVLPDDVVQYIKAHTGGTLDDGIRQGYPTEGTGFPENSKLPSPGLQGHRRLAAADSNMGPSAPGLGGCQLSYSPSSNLNKSNMPVQWNEVSSGTVDALPTQVKPPPFPHSNLAVVQQKPAFGQYPGYNPQSVQSSSGGLDSTQPHLQLRGAPSASRGSYTQQPRQPAAGSQCLGMSAAMSPQASYSQAHPQLSPNIVSGSLNQFSPSCSNMAAKPSHLGLPQQMEVVPNATIMNGHQREHGVPNSSLAAVSQPHPVLSYPQQDSYQQGSNLLSSHQPGFMESQQNAGFGLMQPRPPLEPNTASRHRGVRSGQQQLYARTTGQAMVTSANQETAEAMPKGPAGTMVSLAPQPSQDTGRAQDQNTLYYYGQIHMYEQNGGCPAVQPQPPQPQACSDSIQPEPLPSPGVNQVSSTVDSQLLEPPQIDFDAIMDDGDHSSLFSGALSPTLLHNLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	XM_030248278.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248278.1

ORF Insert Sequence:

ATGGAGACTT CTGCCCCAGC CCCTGCACTG GAGAAGAAAG AAGCCAAGAG TGGTCTCTTG 
GAGGACAGCA GCTTCCCCGA CCCAGGGAAA AAGGCCTGTC CTCTGGCGGT GGCCGCAGCT 
GTAGCCGCCC ACGGAGTTCC TCAGCAGCTC CTGCCGGCTT TCCACGCGCC TTTGCCGATT 
GACATGAGAC ACCAGGAGGG AAGGTACCAT TATGACCCTC ACTCTGTCCA CAGTGTACAC 
GGGCCTCCCA CCCTAAGTGG CAGCCCTGTC ATCTCAGATA TCTCCTTGAT ACGACTTTCT 
CCACACCCTG CTGGCCCTGG AGAGTCACCC TTCAGCGCCC ACCACCCCTA CGTGAACCCC 
CATATGGAGC ACTACCTCCG GTCTGTGCAC AGCAGCCCCA CACTCTCAAT GATCTCTGCC 
GCCAGGGGCC TCAGCCCTGC TGATGTGGCC CACGAACATC TGAAAGAGAG GGGACTCTTT 
AGCCTCGCAG CCCCAGGCAC CAACCCTTCA GACTATTACC ACCAGATGAC CCTCATGGCA 
AGCCACCCCA CCCCTTATGG GGACCTTCTA ATGCAGAGCG GGGGTGCTGC TAGCGCACCC 
CATCTCCATG ACTACCTCAA CCCTGTGGAT GCATCACGAT TCTCTAGTCC ACGTGTGACC 
CCACGACTGA GCCGCAAGCG GGCTCTGTCC ATCTCCCCGC TCTCAGATGC CAGCCTCGAC 
CTACAACGCA TGATTCGGAC CTCTCCCAAC TCGCTGGTAG CTTACATCAA CAACTCCAGG 
AGCAGCTCAG CAGCCAGTGG CTCTTATGGA CATCTGTCTG CTGGTGCCCT CAGCCCAGCC 
TTCACTTTTC CCCACCCCAT CAATCCTGTG GCCTACCAGC AGATCCTGAG CCAGCAGCGG 
GGCCTGGGCT CAGCCTTTGG ACACACACCA CCCCTGATCC AGCCTTCACC CACCTTCTTG 
GCCCAGCAGC CCATGACTCT CACCTCCATC AGCACCATGC CTACCCAACT CAGCAGCAGT 
AGCAGCAACT GTCTAAATGA TGCCAACCAG AACAAGCAGA ACAGCGAGTC AGCTGTGAGC 
AGCACCGTGA ACCCCATCAC CATTCATAAG CGGAGCAAGG TCAAGACTGA GGCTGAGGGC 
CTGCGTCCAG CATCCCCGCT TGGACTGACA CAGGAGCAGC TGGCCGATCT CAAGGAAGAC 
CTGGACAGGG ATGACTGTAA GCAGGAGGCC GAGGTGGTCA TCTACGAGAC CAACTGCCAC 
TGGGCAGACT GCACCAAGGA GTATGACACA CAGGAGCAGC TGGTGCATCA TATCAACAAT 
GAACACATCC ACGGGGAGAA GAAGGAGTTC GTGTGCCGCT GGCAGGCCTG CACGAGAGAG 
CAGAAGCCCT TCAAGGCCCA GTACATGCTG GTTGTTCACA TGCGCAGACA CACGGGTGAG 
AAGCCACACA AGTGCACGTT CGAAGGCTGT TCCAAGGCCT ACTCTCGCCT GGAGAACCTG 
AAGACACACC TGCGTTCACA CACAGGAGAG AAGCCATATG TGTGTGAACA CGAAGGCTGT 
AACAAAGCCT TCTCCAATGC CTCAGACCGC GCCAAGCACC AGAACCGCAC TCACTCCAAT 
GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GGTACACAGA CCCCAGCTCA 
CTCCGCAAGC ATGTGAAGAC TGTCCATGGG CCAGACGCCC ATGTCACCAA GAAACAGCGT 
AATGATGTGC ATGTCCGTGC TCCACTGCTC AAGGAGAATG GGGATAATGA GGCCAGCGCC 
GAGCCAGGTG GCCGGGGACC TGAGGAGAGT GTGGAGGCCA GTAGCACCAG CCACACTGTG 
GAGGACTGCC TACATATCAA AGCCATCAAG ACAGAGAGCT CCGGGCTTTG TCAGTCCAGC 
CCCGGGGCCC AGTCATCCTG CAGCAGCGAG CCCTCTCCCC TGGGCAGTGC CCCCAACAAT 
GACAGTGGCA TGGAGATGCC GGGGACAGGG CCTGGGAGTC TGGGAGACCT GACAGCACTG 
GCTGACACGT GTCCAGGAGC TGACACCTCA GCCCTGGCTG CACCCTCCAC TGGTGGCCTG 
CAGCTGCGCA AACACATGAG CACCGTGCAT CGCTTTGAGC AGCTGAAGAG AGAGAAGCTC 
AAGTCACTGA AGGATTCCTG CTCGTGGGCC GGCCCAGCTC CACACACCCG CAACACCAAG 
CTGCCTCCCC TTCCAGTCAA TGGTTCTGTC CTGGAAAACT TCAACAATAC AGGGGGCGGT 
GGACCGGCAG GACTGCTGCC CAGCCAGCGG CTACCAGAGC TGACCGAAGT GACGATGCTG 
AGCCAGCTGC AGGAACGAAG AGACAGCTCC ACCAGCACCA TGAGCTCGGC CTACACTGTG 
AGCCGCCGCT CCTCTGGCAT CTCCCCATAC TTCTCTAGCC GTCGCTCCAG CGAGGCTTCG 
CCTCTCGGTG GCCTACGCCC GCACAACGCC AGCTCAGCAG ACTCCTATGA CCCCATCTCC 
ACAGATGCCT CTCGGCGCTC CAGTGAAGCC AGCCAGTGCA GTGGCGGTGG CCCAGGGCTG 
CTCAACCTCA CACCTGCGCA GCAGTACAAC CTGCGTGCCA AGTACGCAGC GGCCACAGGT 
GGACCACCGC CCACGCCACT GCCGGGCCTC GATCGTGTAA GCCTTCGTAC CCGCCTGGCC 
TTGCTGGATG CTCCTGAGCG TGCACTTCCT GGTGCCTGCC CACATCCACT GGGGCCACGG 
CGTGGCAGCG ATGGGCCTAC CTATAGCCAT GGTCATGGCC ATGGCTACGC AGGTGCGGCT 
CCAGCATTCC CCCACGAGGG GCCAAACAGC AGCACACGGC GGGCCAGCGA CCCTGTGCGG 
CGCCCTGACC CCCTTATTCT GCCTCGAGTG CAACGTTTCC ACAGTACCCA CAACATGAAT 
CCAGGTTCAC TGCCACCCTG CGCTGATCGG CGTGGCCTGC ACGTACAGAG CCACCCCAGC 
GTAGACAGCA ACCTGACCCG CAACGCCTAC TCTCCCAGAC CCCCTAGCAT CAATGAGAAC 
GTGGTGATGG AGGCCGTGGC TGCTGGGGTA GACGGCCCAG GGCTAGAGTG CGACCTGGGG 
CTGGTGGAGG ATGAGCTGGT GCTGCCAGAT GATGTGGTAC AGTACATCAA GGCTCACACC 
GGTGGTACCT TGGATGACGG CATTCGGCAG GGGTATCCCA CAGAAGGTAC TGGCTTCCCC 
GAGAACTCTA AGCTGCCCAG TCCTGGGCTA CAAGGCCACC GCAGGCTAGC AGCTGCCGAC 
TCCAACATGG GTCCTTCTGC TCCTGGACTC GGGGGCTGCC AGCTGAGCTA CAGCCCCTCC 
TCCAACCTCA ACAAGAGCAA CATGCCTGTG CAGTGGAATG AGGTGAGTTC TGGCACCGTG 
GATGCCCTGC CTACCCAGGT GAAGCCACCT CCTTTCCCTC ACAGCAACCT GGCTGTGGTC 
CAACAGAAGC CAGCCTTTGG CCAGTATCCA GGATATAATC CACAATCCGT GCAGAGCAGC 
TCCGGAGGTC TAGACAGCAC CCAGCCGCAC CTACAGCTTC GAGGAGCCCC CTCTGCATCA 
AGAGGGAGCT ACACGCAACA GCCTCGACAG CCAGCTGCAG GCAGTCAGTG CCTGGGTATG 
AGTGCGGCCA TGAGCCCGCA GGCCAGCTAC AGCCAAGCCC ACCCCCAGCT GAGCCCAAAC 
ATTGTCAGCG GATCTCTGAA CCAGTTTTCT CCCTCCTGCA GCAATATGGC AGCCAAGCCC 
AGCCACCTGG GACTCCCTCA GCAAATGGAA GTTGTCCCCA ATGCCACCAT CATGAATGGC 
CATCAACGGG AGCACGGGGT CCCCAATTCA TCCCTGGCTG CGGTGTCACA ACCTCACCCA 
GTCCTGAGCT ATCCCCAGCA GGACAGCTAC CAACAGGGCT CCAACCTTCT GTCATCCCAT 
CAGCCTGGCT TCATGGAGTC CCAGCAGAAC GCGGGCTTTG GTCTCATGCA GCCTCGGCCA 
CCCCTGGAAC CCAACACGGC CAGCCGTCAC CGTGGAGTAC GTTCTGGGCA ACAGCAGTTG 
TATGCCAGGA CCACTGGCCA AGCCATGGTC ACATCAGCCA ACCAAGAGAC AGCAGAAGCT 
ATGCCCAAGG GACCAGCAGG GACCATGGTA TCCCTAGCTC CTCAGCCATC TCAGGACACA 
GGGCGGGCAC AAGATCAGAA CACGCTATAC TACTATGGCC AGATCCACAT GTATGAACAG 
AATGGAGGCT GCCCAGCCGT GCAGCCCCAG CCGCCACAAC CACAAGCTTG CTCAGACAGT 
ATCCAGCCTG AGCCTTTGCC TTCACCGGGA GTCAACCAGG TGTCTAGCAC CGTGGACTCC 
CAGCTCCTGG AGCCCCCCCA GATTGACTTT GATGCCATCA TGGATGATGG TGATCACTCG 
AGTTTGTTTT CTGGTGCACT GAGCCCAACC CTTCTCCACA ATCTCTCCCA GAATTCCTCA 
CGCCTCACCA CACCCCGGAA TTCCTTGACA CTGCCCTCCA TCCCTGCGGG CATCAGCAAC 
ATGGCCGTGG GCGACATGAG TTCCATGCTC ACCAGCCTGG CTGAAGAGAG CAAGTTTTTA 
AACATGATGA CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gli2 ( NM_001081125.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074594.1 Mus musculus GLI-Kruppel family member GLI2 (Gli2), mRNA.

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CloneID	OMu22271
Clone ID Related Accession (Same CDS sequence)	XM_030248278.1 , NM_001081125.1
Accession Version	NM_001081125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	zinc finger protein GLI2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ102399.1, BC085190.1, AM262744.1 and AK051858.1. On Feb 6, 2007 this sequence version replaced XM_136212.7. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC082604.1, X99104.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACTT CTGCCCCAGC CCCTGCACTG GAGAAGAAAG AAGCCAAGAG TGGTCTCTTG 
GAGGACAGCA GCTTCCCCGA CCCAGGGAAA AAGGCCTGTC CTCTGGCGGT GGCCGCAGCT 
GTAGCCGCCC ACGGAGTTCC TCAGCAGCTC CTGCCGGCTT TCCACGCGCC TTTGCCGATT 
GACATGAGAC ACCAGGAGGG AAGGTACCAT TATGACCCTC ACTCTGTCCA CAGTGTACAC 
GGGCCTCCCA CCCTAAGTGG CAGCCCTGTC ATCTCAGATA TCTCCTTGAT ACGACTTTCT 
CCACACCCTG CTGGCCCTGG AGAGTCACCC TTCAGCGCCC ACCACCCCTA CGTGAACCCC 
CATATGGAGC ACTACCTCCG GTCTGTGCAC AGCAGCCCCA CACTCTCAAT GATCTCTGCC 
GCCAGGGGCC TCAGCCCTGC TGATGTGGCC CACGAACATC TGAAAGAGAG GGGACTCTTT 
AGCCTCGCAG CCCCAGGCAC CAACCCTTCA GACTATTACC ACCAGATGAC CCTCATGGCA 
AGCCACCCCA CCCCTTATGG GGACCTTCTA ATGCAGAGCG GGGGTGCTGC TAGCGCACCC 
CATCTCCATG ACTACCTCAA CCCTGTGGAT GCATCACGAT TCTCTAGTCC ACGTGTGACC 
CCACGACTGA GCCGCAAGCG GGCTCTGTCC ATCTCCCCGC TCTCAGATGC CAGCCTCGAC 
CTACAACGCA TGATTCGGAC CTCTCCCAAC TCGCTGGTAG CTTACATCAA CAACTCCAGG 
AGCAGCTCAG CAGCCAGTGG CTCTTATGGA CATCTGTCTG CTGGTGCCCT CAGCCCAGCC 
TTCACTTTTC CCCACCCCAT CAATCCTGTG GCCTACCAGC AGATCCTGAG CCAGCAGCGG 
GGCCTGGGCT CAGCCTTTGG ACACACACCA CCCCTGATCC AGCCTTCACC CACCTTCTTG 
GCCCAGCAGC CCATGACTCT CACCTCCATC AGCACCATGC CTACCCAACT CAGCAGCAGT 
AGCAGCAACT GTCTAAATGA TGCCAACCAG AACAAGCAGA ACAGCGAGTC AGCTGTGAGC 
AGCACCGTGA ACCCCATCAC CATTCATAAG CGGAGCAAGG TCAAGACTGA GGCTGAGGGC 
CTGCGTCCAG CATCCCCGCT TGGACTGACA CAGGAGCAGC TGGCCGATCT CAAGGAAGAC 
CTGGACAGGG ATGACTGTAA GCAGGAGGCC GAGGTGGTCA TCTACGAGAC CAACTGCCAC 
TGGGCAGACT GCACCAAGGA GTATGACACA CAGGAGCAGC TGGTGCATCA TATCAACAAT 
GAACACATCC ACGGGGAGAA GAAGGAGTTC GTGTGCCGCT GGCAGGCCTG CACGAGAGAG 
CAGAAGCCCT TCAAGGCCCA GTACATGCTG GTTGTTCACA TGCGCAGACA CACGGGTGAG 
AAGCCACACA AGTGCACGTT CGAAGGCTGT TCCAAGGCCT ACTCTCGCCT GGAGAACCTG 
AAGACACACC TGCGTTCACA CACAGGAGAG AAGCCATATG TGTGTGAACA CGAAGGCTGT 
AACAAAGCCT TCTCCAATGC CTCAGACCGC GCCAAGCACC AGAACCGCAC TCACTCCAAT 
GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GGTACACAGA CCCCAGCTCA 
CTCCGCAAGC ATGTGAAGAC TGTCCATGGG CCAGACGCCC ATGTCACCAA GAAACAGCGT 
AATGATGTGC ATGTCCGTGC TCCACTGCTC AAGGAGAATG GGGATAATGA GGCCAGCGCC 
GAGCCAGGTG GCCGGGGACC TGAGGAGAGT GTGGAGGCCA GTAGCACCAG CCACACTGTG 
GAGGACTGCC TACATATCAA AGCCATCAAG ACAGAGAGCT CCGGGCTTTG TCAGTCCAGC 
CCCGGGGCCC AGTCATCCTG CAGCAGCGAG CCCTCTCCCC TGGGCAGTGC CCCCAACAAT 
GACAGTGGCA TGGAGATGCC GGGGACAGGG CCTGGGAGTC TGGGAGACCT GACAGCACTG 
GCTGACACGT GTCCAGGAGC TGACACCTCA GCCCTGGCTG CACCCTCCAC TGGTGGCCTG 
CAGCTGCGCA AACACATGAG CACCGTGCAT CGCTTTGAGC AGCTGAAGAG AGAGAAGCTC 
AAGTCACTGA AGGATTCCTG CTCGTGGGCC GGCCCAGCTC CACACACCCG CAACACCAAG 
CTGCCTCCCC TTCCAGTCAA TGGTTCTGTC CTGGAAAACT TCAACAATAC AGGGGGCGGT 
GGACCGGCAG GACTGCTGCC CAGCCAGCGG CTACCAGAGC TGACCGAAGT GACGATGCTG 
AGCCAGCTGC AGGAACGAAG AGACAGCTCC ACCAGCACCA TGAGCTCGGC CTACACTGTG 
AGCCGCCGCT CCTCTGGCAT CTCCCCATAC TTCTCTAGCC GTCGCTCCAG CGAGGCTTCG 
CCTCTCGGTG GCCTACGCCC GCACAACGCC AGCTCAGCAG ACTCCTATGA CCCCATCTCC 
ACAGATGCCT CTCGGCGCTC CAGTGAAGCC AGCCAGTGCA GTGGCGGTGG CCCAGGGCTG 
CTCAACCTCA CACCTGCGCA GCAGTACAAC CTGCGTGCCA AGTACGCAGC GGCCACAGGT 
GGACCACCGC CCACGCCACT GCCGGGCCTC GATCGTGTAA GCCTTCGTAC CCGCCTGGCC 
TTGCTGGATG CTCCTGAGCG TGCACTTCCT GGTGCCTGCC CACATCCACT GGGGCCACGG 
CGTGGCAGCG ATGGGCCTAC CTATAGCCAT GGTCATGGCC ATGGCTACGC AGGTGCGGCT 
CCAGCATTCC CCCACGAGGG GCCAAACAGC AGCACACGGC GGGCCAGCGA CCCTGTGCGG 
CGCCCTGACC CCCTTATTCT GCCTCGAGTG CAACGTTTCC ACAGTACCCA CAACATGAAT 
CCAGGTTCAC TGCCACCCTG CGCTGATCGG CGTGGCCTGC ACGTACAGAG CCACCCCAGC 
GTAGACAGCA ACCTGACCCG CAACGCCTAC TCTCCCAGAC CCCCTAGCAT CAATGAGAAC 
GTGGTGATGG AGGCCGTGGC TGCTGGGGTA GACGGCCCAG GGCTAGAGTG CGACCTGGGG 
CTGGTGGAGG ATGAGCTGGT GCTGCCAGAT GATGTGGTAC AGTACATCAA GGCTCACACC 
GGTGGTACCT TGGATGACGG CATTCGGCAG GGGTATCCCA CAGAAGGTAC TGGCTTCCCC 
GAGAACTCTA AGCTGCCCAG TCCTGGGCTA CAAGGCCACC GCAGGCTAGC AGCTGCCGAC 
TCCAACATGG GTCCTTCTGC TCCTGGACTC GGGGGCTGCC AGCTGAGCTA CAGCCCCTCC 
TCCAACCTCA ACAAGAGCAA CATGCCTGTG CAGTGGAATG AGGTGAGTTC TGGCACCGTG 
GATGCCCTGC CTACCCAGGT GAAGCCACCT CCTTTCCCTC ACAGCAACCT GGCTGTGGTC 
CAACAGAAGC CAGCCTTTGG CCAGTATCCA GGATATAATC CACAATCCGT GCAGAGCAGC 
TCCGGAGGTC TAGACAGCAC CCAGCCGCAC CTACAGCTTC GAGGAGCCCC CTCTGCATCA 
AGAGGGAGCT ACACGCAACA GCCTCGACAG CCAGCTGCAG GCAGTCAGTG CCTGGGTATG 
AGTGCGGCCA TGAGCCCGCA GGCCAGCTAC AGCCAAGCCC ACCCCCAGCT GAGCCCAAAC 
ATTGTCAGCG GATCTCTGAA CCAGTTTTCT CCCTCCTGCA GCAATATGGC AGCCAAGCCC 
AGCCACCTGG GACTCCCTCA GCAAATGGAA GTTGTCCCCA ATGCCACCAT CATGAATGGC 
CATCAACGGG AGCACGGGGT CCCCAATTCA TCCCTGGCTG CGGTGTCACA ACCTCACCCA 
GTCCTGAGCT ATCCCCAGCA GGACAGCTAC CAACAGGGCT CCAACCTTCT GTCATCCCAT 
CAGCCTGGCT TCATGGAGTC CCAGCAGAAC GCGGGCTTTG GTCTCATGCA GCCTCGGCCA 
CCCCTGGAAC CCAACACGGC CAGCCGTCAC CGTGGAGTAC GTTCTGGGCA ACAGCAGTTG 
TATGCCAGGA CCACTGGCCA AGCCATGGTC ACATCAGCCA ACCAAGAGAC AGCAGAAGCT 
ATGCCCAAGG GACCAGCAGG GACCATGGTA TCCCTAGCTC CTCAGCCATC TCAGGACACA 
GGGCGGGCAC AAGATCAGAA CACGCTATAC TACTATGGCC AGATCCACAT GTATGAACAG 
AATGGAGGCT GCCCAGCCGT GCAGCCCCAG CCGCCACAAC CACAAGCTTG CTCAGACAGT 
ATCCAGCCTG AGCCTTTGCC TTCACCGGGA GTCAACCAGG TGTCTAGCAC CGTGGACTCC 
CAGCTCCTGG AGCCCCCCCA GATTGACTTT GATGCCATCA TGGATGATGG TGATCACTCG 
AGTTTGTTTT CTGGTGCACT GAGCCCAACC CTTCTCCACA ATCTCTCCCA GAATTCCTCA 
CGCCTCACCA CACCCCGGAA TTCCTTGACA CTGCCCTCCA TCCCTGCGGG CATCAGCAAC 
ATGGCCGTGG GCGACATGAG TTCCATGCTC ACCAGCCTGG CTGAAGAGAG CAAGTTTTTA 
AACATGATGA CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074594.1
CDS	350..4984
Translation	METSAPAPALEKKEAKSGLLEDSSFPDPGKKACPLAVAAAVAAHGVPQQLLPAFHAPLPIDMRHQEGRYHYDPHSVHSVHGPPTLSGSPVISDISLIRLSPHPAGPGESPFSAHHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAHEHLKERGLFSLAAPGTNPSDYYHQMTLMASHPTPYGDLLMQSGGAASAPHLHDYLNPVDASRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMTLTSISTMPTQLSSSSSNCLNDANQNKQNSESAVSSTVNPITIHKRSKVKTEAEGLRPASPLGLTQEQLADLKEDLDRDDCKQEAEVVIYETNCHWADCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHVRAPLLKENGDNEASAEPGGRGPEESVEASSTSHTVEDCLHIKAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGMEMPGTGPGSLGDLTALADTCPGADTSALAAPSTGGLQLRKHMSTVHRFEQLKREKLKSLKDSCSWAGPAPHTRNTKLPPLPVNGSVLENFNNTGGGGPAGLLPSQRLPELTEVTMLSQLQERRDSSTSTMSSAYTVSRRSSGISPYFSSRRSSEASPLGGLRPHNASSADSYDPISTDASRRSSEASQCSGGGPGLLNLTPAQQYNLRAKYAAATGGPPPTPLPGLDRVSLRTRLALLDAPERALPGACPHPLGPRRGSDGPTYSHGHGHGYAGAAPAFPHEGPNSSTRRASDPVRRPDPLILPRVQRFHSTHNMNPGSLPPCADRRGLHVQSHPSVDSNLTRNAYSPRPPSINENVVMEAVAAGVDGPGLECDLGLVEDELVLPDDVVQYIKAHTGGTLDDGIRQGYPTEGTGFPENSKLPSPGLQGHRRLAAADSNMGPSAPGLGGCQLSYSPSSNLNKSNMPVQWNEVSSGTVDALPTQVKPPPFPHSNLAVVQQKPAFGQYPGYNPQSVQSSSGGLDSTQPHLQLRGAPSASRGSYTQQPRQPAAGSQCLGMSAAMSPQASYSQAHPQLSPNIVSGSLNQFSPSCSNMAAKPSHLGLPQQMEVVPNATIMNGHQREHGVPNSSLAAVSQPHPVLSYPQQDSYQQGSNLLSSHQPGFMESQQNAGFGLMQPRPPLEPNTASRHRGVRSGQQQLYARTTGQAMVTSANQETAEAMPKGPAGTMVSLAPQPSQDTGRAQDQNTLYYYGQIHMYEQNGGCPAVQPQPPQPQACSDSIQPEPLPSPGVNQVSSTVDSQLLEPPQIDFDAIMDDGDHSSLFSGALSPTLLHNLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	NM_001081125.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GLI-Kruppel family member GLI2 (Gli2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081125.1

ORF Insert Sequence:

ATGGAGACTT CTGCCCCAGC CCCTGCACTG GAGAAGAAAG AAGCCAAGAG TGGTCTCTTG 
GAGGACAGCA GCTTCCCCGA CCCAGGGAAA AAGGCCTGTC CTCTGGCGGT GGCCGCAGCT 
GTAGCCGCCC ACGGAGTTCC TCAGCAGCTC CTGCCGGCTT TCCACGCGCC TTTGCCGATT 
GACATGAGAC ACCAGGAGGG AAGGTACCAT TATGACCCTC ACTCTGTCCA CAGTGTACAC 
GGGCCTCCCA CCCTAAGTGG CAGCCCTGTC ATCTCAGATA TCTCCTTGAT ACGACTTTCT 
CCACACCCTG CTGGCCCTGG AGAGTCACCC TTCAGCGCCC ACCACCCCTA CGTGAACCCC 
CATATGGAGC ACTACCTCCG GTCTGTGCAC AGCAGCCCCA CACTCTCAAT GATCTCTGCC 
GCCAGGGGCC TCAGCCCTGC TGATGTGGCC CACGAACATC TGAAAGAGAG GGGACTCTTT 
AGCCTCGCAG CCCCAGGCAC CAACCCTTCA GACTATTACC ACCAGATGAC CCTCATGGCA 
AGCCACCCCA CCCCTTATGG GGACCTTCTA ATGCAGAGCG GGGGTGCTGC TAGCGCACCC 
CATCTCCATG ACTACCTCAA CCCTGTGGAT GCATCACGAT TCTCTAGTCC ACGTGTGACC 
CCACGACTGA GCCGCAAGCG GGCTCTGTCC ATCTCCCCGC TCTCAGATGC CAGCCTCGAC 
CTACAACGCA TGATTCGGAC CTCTCCCAAC TCGCTGGTAG CTTACATCAA CAACTCCAGG 
AGCAGCTCAG CAGCCAGTGG CTCTTATGGA CATCTGTCTG CTGGTGCCCT CAGCCCAGCC 
TTCACTTTTC CCCACCCCAT CAATCCTGTG GCCTACCAGC AGATCCTGAG CCAGCAGCGG 
GGCCTGGGCT CAGCCTTTGG ACACACACCA CCCCTGATCC AGCCTTCACC CACCTTCTTG 
GCCCAGCAGC CCATGACTCT CACCTCCATC AGCACCATGC CTACCCAACT CAGCAGCAGT 
AGCAGCAACT GTCTAAATGA TGCCAACCAG AACAAGCAGA ACAGCGAGTC AGCTGTGAGC 
AGCACCGTGA ACCCCATCAC CATTCATAAG CGGAGCAAGG TCAAGACTGA GGCTGAGGGC 
CTGCGTCCAG CATCCCCGCT TGGACTGACA CAGGAGCAGC TGGCCGATCT CAAGGAAGAC 
CTGGACAGGG ATGACTGTAA GCAGGAGGCC GAGGTGGTCA TCTACGAGAC CAACTGCCAC 
TGGGCAGACT GCACCAAGGA GTATGACACA CAGGAGCAGC TGGTGCATCA TATCAACAAT 
GAACACATCC ACGGGGAGAA GAAGGAGTTC GTGTGCCGCT GGCAGGCCTG CACGAGAGAG 
CAGAAGCCCT TCAAGGCCCA GTACATGCTG GTTGTTCACA TGCGCAGACA CACGGGTGAG 
AAGCCACACA AGTGCACGTT CGAAGGCTGT TCCAAGGCCT ACTCTCGCCT GGAGAACCTG 
AAGACACACC TGCGTTCACA CACAGGAGAG AAGCCATATG TGTGTGAACA CGAAGGCTGT 
AACAAAGCCT TCTCCAATGC CTCAGACCGC GCCAAGCACC AGAACCGCAC TCACTCCAAT 
GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GGTACACAGA CCCCAGCTCA 
CTCCGCAAGC ATGTGAAGAC TGTCCATGGG CCAGACGCCC ATGTCACCAA GAAACAGCGT 
AATGATGTGC ATGTCCGTGC TCCACTGCTC AAGGAGAATG GGGATAATGA GGCCAGCGCC 
GAGCCAGGTG GCCGGGGACC TGAGGAGAGT GTGGAGGCCA GTAGCACCAG CCACACTGTG 
GAGGACTGCC TACATATCAA AGCCATCAAG ACAGAGAGCT CCGGGCTTTG TCAGTCCAGC 
CCCGGGGCCC AGTCATCCTG CAGCAGCGAG CCCTCTCCCC TGGGCAGTGC CCCCAACAAT 
GACAGTGGCA TGGAGATGCC GGGGACAGGG CCTGGGAGTC TGGGAGACCT GACAGCACTG 
GCTGACACGT GTCCAGGAGC TGACACCTCA GCCCTGGCTG CACCCTCCAC TGGTGGCCTG 
CAGCTGCGCA AACACATGAG CACCGTGCAT CGCTTTGAGC AGCTGAAGAG AGAGAAGCTC 
AAGTCACTGA AGGATTCCTG CTCGTGGGCC GGCCCAGCTC CACACACCCG CAACACCAAG 
CTGCCTCCCC TTCCAGTCAA TGGTTCTGTC CTGGAAAACT TCAACAATAC AGGGGGCGGT 
GGACCGGCAG GACTGCTGCC CAGCCAGCGG CTACCAGAGC TGACCGAAGT GACGATGCTG 
AGCCAGCTGC AGGAACGAAG AGACAGCTCC ACCAGCACCA TGAGCTCGGC CTACACTGTG 
AGCCGCCGCT CCTCTGGCAT CTCCCCATAC TTCTCTAGCC GTCGCTCCAG CGAGGCTTCG 
CCTCTCGGTG GCCTACGCCC GCACAACGCC AGCTCAGCAG ACTCCTATGA CCCCATCTCC 
ACAGATGCCT CTCGGCGCTC CAGTGAAGCC AGCCAGTGCA GTGGCGGTGG CCCAGGGCTG 
CTCAACCTCA CACCTGCGCA GCAGTACAAC CTGCGTGCCA AGTACGCAGC GGCCACAGGT 
GGACCACCGC CCACGCCACT GCCGGGCCTC GATCGTGTAA GCCTTCGTAC CCGCCTGGCC 
TTGCTGGATG CTCCTGAGCG TGCACTTCCT GGTGCCTGCC CACATCCACT GGGGCCACGG 
CGTGGCAGCG ATGGGCCTAC CTATAGCCAT GGTCATGGCC ATGGCTACGC AGGTGCGGCT 
CCAGCATTCC CCCACGAGGG GCCAAACAGC AGCACACGGC GGGCCAGCGA CCCTGTGCGG 
CGCCCTGACC CCCTTATTCT GCCTCGAGTG CAACGTTTCC ACAGTACCCA CAACATGAAT 
CCAGGTTCAC TGCCACCCTG CGCTGATCGG CGTGGCCTGC ACGTACAGAG CCACCCCAGC 
GTAGACAGCA ACCTGACCCG CAACGCCTAC TCTCCCAGAC CCCCTAGCAT CAATGAGAAC 
GTGGTGATGG AGGCCGTGGC TGCTGGGGTA GACGGCCCAG GGCTAGAGTG CGACCTGGGG 
CTGGTGGAGG ATGAGCTGGT GCTGCCAGAT GATGTGGTAC AGTACATCAA GGCTCACACC 
GGTGGTACCT TGGATGACGG CATTCGGCAG GGGTATCCCA CAGAAGGTAC TGGCTTCCCC 
GAGAACTCTA AGCTGCCCAG TCCTGGGCTA CAAGGCCACC GCAGGCTAGC AGCTGCCGAC 
TCCAACATGG GTCCTTCTGC TCCTGGACTC GGGGGCTGCC AGCTGAGCTA CAGCCCCTCC 
TCCAACCTCA ACAAGAGCAA CATGCCTGTG CAGTGGAATG AGGTGAGTTC TGGCACCGTG 
GATGCCCTGC CTACCCAGGT GAAGCCACCT CCTTTCCCTC ACAGCAACCT GGCTGTGGTC 
CAACAGAAGC CAGCCTTTGG CCAGTATCCA GGATATAATC CACAATCCGT GCAGAGCAGC 
TCCGGAGGTC TAGACAGCAC CCAGCCGCAC CTACAGCTTC GAGGAGCCCC CTCTGCATCA 
AGAGGGAGCT ACACGCAACA GCCTCGACAG CCAGCTGCAG GCAGTCAGTG CCTGGGTATG 
AGTGCGGCCA TGAGCCCGCA GGCCAGCTAC AGCCAAGCCC ACCCCCAGCT GAGCCCAAAC 
ATTGTCAGCG GATCTCTGAA CCAGTTTTCT CCCTCCTGCA GCAATATGGC AGCCAAGCCC 
AGCCACCTGG GACTCCCTCA GCAAATGGAA GTTGTCCCCA ATGCCACCAT CATGAATGGC 
CATCAACGGG AGCACGGGGT CCCCAATTCA TCCCTGGCTG CGGTGTCACA ACCTCACCCA 
GTCCTGAGCT ATCCCCAGCA GGACAGCTAC CAACAGGGCT CCAACCTTCT GTCATCCCAT 
CAGCCTGGCT TCATGGAGTC CCAGCAGAAC GCGGGCTTTG GTCTCATGCA GCCTCGGCCA 
CCCCTGGAAC CCAACACGGC CAGCCGTCAC CGTGGAGTAC GTTCTGGGCA ACAGCAGTTG 
TATGCCAGGA CCACTGGCCA AGCCATGGTC ACATCAGCCA ACCAAGAGAC AGCAGAAGCT 
ATGCCCAAGG GACCAGCAGG GACCATGGTA TCCCTAGCTC CTCAGCCATC TCAGGACACA 
GGGCGGGCAC AAGATCAGAA CACGCTATAC TACTATGGCC AGATCCACAT GTATGAACAG 
AATGGAGGCT GCCCAGCCGT GCAGCCCCAG CCGCCACAAC CACAAGCTTG CTCAGACAGT 
ATCCAGCCTG AGCCTTTGCC TTCACCGGGA GTCAACCAGG TGTCTAGCAC CGTGGACTCC 
CAGCTCCTGG AGCCCCCCCA GATTGACTTT GATGCCATCA TGGATGATGG TGATCACTCG 
AGTTTGTTTT CTGGTGCACT GAGCCCAACC CTTCTCCACA ATCTCTCCCA GAATTCCTCA 
CGCCTCACCA CACCCCGGAA TTCCTTGACA CTGCCCTCCA TCCCTGCGGG CATCAGCAAC 
ATGGCCGTGG GCGACATGAG TTCCATGCTC ACCAGCCTGG CTGAAGAGAG CAAGTTTTTA 
AACATGATGA CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gli2 ( XM_011247924.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246226.1 Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu27904
Clone ID Related Accession (Same CDS sequence)	XM_011247924.2 , XM_006529141.3 , XM_006529141.4 , XM_011247924.3
Accession Version	XM_011247924.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	zinc finger protein GLI2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247924.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACACC AGGAGGGAAG GTACCATTAT GACCCTCACT CTGTCCACAG TGTACACGGG 
CCTCCCACCC TAAGTGGCAG CCCTGTCATC TCAGATATCT CCTTGATACG ACTTTCTCCA 
CACCCTGCTG GCCCTGGAGA GTCACCCTTC AGCGCCCACC ACCCCTACGT GAACCCCCAT 
ATGGAGCACT ACCTCCGGTC TGTGCACAGC AGCCCCACAC TCTCAATGAT CTCTGCCGCC 
AGGGGCCTCA GCCCTGCTGA TGTGGCCCAC GAACATCTGA AAGAGAGGGG ACTCTTTAGC 
CTCGCAGCCC CAGGCACCAA CCCTTCAGAC TATTACCACC AGATGACCCT CATGGCAAGC 
CACCCCACCC CTTATGGGGA CCTTCTAATG CAGAGCGGGG GTGCTGCTAG CGCACCCCAT 
CTCCATGACT ACCTCAACCC TGTGGATGCA TCACGATTCT CTAGTCCACG TGTGACCCCA 
CGACTGAGCC GCAAGCGGGC TCTGTCCATC TCCCCGCTCT CAGATGCCAG CCTCGACCTA 
CAACGCATGA TTCGGACCTC TCCCAACTCG CTGGTAGCTT ACATCAACAA CTCCAGGAGC 
AGCTCAGCAG CCAGTGGCTC TTATGGACAT CTGTCTGCTG GTGCCCTCAG CCCAGCCTTC 
ACTTTTCCCC ACCCCATCAA TCCTGTGGCC TACCAGCAGA TCCTGAGCCA GCAGCGGGGC 
CTGGGCTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CTTCACCCAC CTTCTTGGCC 
CAGCAGCCCA TGACTCTCAC CTCCATCAGC ACCATGCCTA CCCAACTCAG CAGCAGTAGC 
AGCAACTGTC TAAATGATGC CAACCAGAAC AAGCAGAACA GCGAGTCAGC TGTGAGCAGC 
ACCGTGAACC CCATCACCAT TCATAAGCGG AGCAAGGTCA AGACTGAGGC TGAGGGCCTG 
CGTCCAGCAT CCCCGCTTGG ACTGACACAG GAGCAGCTGG CCGATCTCAA GGAAGACCTG 
GACAGGGATG ACTGTAAGCA GGAGGCCGAG GTGGTCATCT ACGAGACCAA CTGCCACTGG 
GCAGACTGCA CCAAGGAGTA TGACACACAG GAGCAGCTGG TGCATCATAT CAACAATGAA 
CACATCCACG GGGAGAAGAA GGAGTTCGTG TGCCGCTGGC AGGCCTGCAC GAGAGAGCAG 
AAGCCCTTCA AGGCCCAGTA CATGCTGGTT GTTCACATGC GCAGACACAC GGGTGAGAAG 
CCACACAAGT GCACGTTCGA AGGCTGTTCC AAGGCCTACT CTCGCCTGGA GAACCTGAAG 
ACACACCTGC GTTCACACAC AGGAGAGAAG CCATATGTGT GTGAACACGA AGGCTGTAAC 
AAAGCCTTCT CCAATGCCTC AGACCGCGCC AAGCACCAGA ACCGCACTCA CTCCAATGAG 
AAACCCTACA TCTGCAAGAT CCCAGGCTGC ACCAAGAGGT ACACAGACCC CAGCTCACTC 
CGCAAGCATG TGAAGACTGT CCATGGGCCA GACGCCCATG TCACCAAGAA ACAGCGTAAT 
GATGTGCATG TCCGTGCTCC ACTGCTCAAG GAGAATGGGG ATAATGAGGC CAGCGCCGAG 
CCAGGTGGCC GGGGACCTGA GGAGAGTGTG GAGGCCAGTA GCACCAGCCA CACTGTGGAG 
GACTGCCTAC ATATCAAAGC CATCAAGACA GAGAGCTCCG GGCTTTGTCA GTCCAGCCCC 
GGGGCCCAGT CATCCTGCAG CAGCGAGCCC TCTCCCCTGG GCAGTGCCCC CAACAATGAC 
AGTGGCATGG AGATGCCGGG GACAGGGCCT GGGAGTCTGG GAGACCTGAC AGCACTGGCT 
GACACGTGTC CAGGAGCTGA CACCTCAGCC CTGGCTGCAC CCTCCACTGG TGGCCTGCAG 
CTGCGCAAAC ACATGAGCAC CGTGCATCGC TTTGAGCAGC TGAAGAGAGA GAAGCTCAAG 
TCACTGAAGG ATTCCTGCTC GTGGGCCGGC CCAGCTCCAC ACACCCGCAA CACCAAGCTG 
CCTCCCCTTC CAGTCAATGG TTCTGTCCTG GAAAACTTCA ACAATACAGG GGGCGGTGGA 
CCGGCAGGAC TGCTGCCCAG CCAGCGGCTA CCAGAGCTGA CCGAAGTGAC GATGCTGAGC 
CAGCTGCAGG AACGAAGAGA CAGCTCCACC AGCACCATGA GCTCGGCCTA CACTGTGAGC 
CGCCGCTCCT CTGGCATCTC CCCATACTTC TCTAGCCGTC GCTCCAGCGA GGCTTCGCCT 
CTCGGTGGCC TACGCCCGCA CAACGCCAGC TCAGCAGACT CCTATGACCC CATCTCCACA 
GATGCCTCTC GGCGCTCCAG TGAAGCCAGC CAGTGCAGTG GCGGTGGCCC AGGGCTGCTC 
AACCTCACAC CTGCGCAGCA GTACAACCTG CGTGCCAAGT ACGCAGCGGC CACAGGTGGA 
CCACCGCCCA CGCCACTGCC GGGCCTCGAT CGTGTAAGCC TTCGTACCCG CCTGGCCTTG 
CTGGATGCTC CTGAGCGTGC ACTTCCTGGT GCCTGCCCAC ATCCACTGGG GCCACGGCGT 
GGCAGCGATG GGCCTACCTA TAGCCATGGT CATGGCCATG GCTACGCAGG TGCGGCTCCA 
GCATTCCCCC ACGAGGGGCC AAACAGCAGC ACACGGCGGG CCAGCGACCC TGTGCGGCGC 
CCTGACCCCC TTATTCTGCC TCGAGTGCAA CGTTTCCACA GTACCCACAA CATGAATCCA 
GGTTCACTGC CACCCTGCGC TGATCGGCGT GGCCTGCACG TACAGAGCCA CCCCAGCGTA 
GACAGCAACC TGACCCGCAA CGCCTACTCT CCCAGACCCC CTAGCATCAA TGAGAACGTG 
GTGATGGAGG CCGTGGCTGC TGGGGTAGAC GGCCCAGGGC TAGAGTGCGA CCTGGGGCTG 
GTGGAGGATG AGCTGGTGCT GCCAGATGAT GTGGTACAGT ACATCAAGGC TCACACCGGT 
GGTACCTTGG ATGACGGCAT TCGGCAGGGG TATCCCACAG AAGGTACTGG CTTCCCCGAG 
AACTCTAAGC TGCCCAGTCC TGGGCTACAA GGCCACCGCA GGCTAGCAGC TGCCGACTCC 
AACATGGGTC CTTCTGCTCC TGGACTCGGG GGCTGCCAGC TGAGCTACAG CCCCTCCTCC 
AACCTCAACA AGAGCAACAT GCCTGTGCAG TGGAATGAGG TGAGTTCTGG CACCGTGGAT 
GCCCTGCCTA CCCAGGTGAA GCCACCTCCT TTCCCTCACA GCAACCTGGC TGTGGTCCAA 
CAGAAGCCAG CCTTTGGCCA GTATCCAGGA TATAATCCAC AATCCGTGCA GAGCAGCTCC 
GGAGGTCTAG ACAGCACCCA GCCGCACCTA CAGCTTCGAG GAGCCCCCTC TGCATCAAGA 
GGGAGCTACA CGCAACAGCC TCGACAGCCA GCTGCAGGCA GTCAGTGCCT GGGTATGAGT 
GCGGCCATGA GCCCGCAGGC CAGCTACAGC CAAGCCCACC CCCAGCTGAG CCCAAACATT 
GTCAGCGGAT CTCTGAACCA GTTTTCTCCC TCCTGCAGCA ATATGGCAGC CAAGCCCAGC 
CACCTGGGAC TCCCTCAGCA AATGGAAGTT GTCCCCAATG CCACCATCAT GAATGGCCAT 
CAACGGGAGC ACGGGGTCCC CAATTCATCC CTGGCTGCGG TGTCACAACC TCACCCAGTC 
CTGAGCTATC CCCAGCAGGA CAGCTACCAA CAGGGCTCCA ACCTTCTGTC ATCCCATCAG 
CCTGGCTTCA TGGAGTCCCA GCAGAACGCG GGCTTTGGTC TCATGCAGCC TCGGCCACCC 
CTGGAACCCA ACACGGCCAG CCGTCACCGT GGAGTACGTT CTGGGCAACA GCAGTTGTAT 
GCCAGGACCA CTGGCCAAGC CATGGTCACA TCAGCCAACC AAGAGACAGC AGAAGCTATG 
CCCAAGGGAC CAGCAGGGAC CATGGTATCC CTAGCTCCTC AGCCATCTCA GGACACAGGG 
CGGGCACAAG ATCAGAACAC GCTATACTAC TATGGCCAGA TCCACATGTA TGAACAGAAT 
GGAGGCTGCC CAGCCGTGCA GCCCCAGCCG CCACAACCAC AAGCTTGCTC AGACAGTATC 
CAGCCTGAGC CTTTGCCTTC ACCGGGAGTC AACCAGGTGT CTAGCACCGT GGACTCCCAG 
CTCCTGGAGC CCCCCCAGAT TGACTTTGAT GCCATCATGG ATGATGGTGA TCACTCGAGT 
TTGTTTTCTG GTGCACTGAG CCCAACCCTT CTCCACAATC TCTCCCAGAA TTCCTCACGC 
CTCACCACAC CCCGGAATTC CTTGACACTG CCCTCCATCC CTGCGGGCAT CAGCAACATG 
GCCGTGGGCG ACATGAGTTC CATGCTCACC AGCCTGGCTG AAGAGAGCAA GTTTTTAAAC 
ATGATGACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246226.1
CDS	355..4806
Translation	MRHQEGRYHYDPHSVHSVHGPPTLSGSPVISDISLIRLSPHPAGPGESPFSAHHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAHEHLKERGLFSLAAPGTNPSDYYHQMTLMASHPTPYGDLLMQSGGAASAPHLHDYLNPVDASRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMTLTSISTMPTQLSSSSSNCLNDANQNKQNSESAVSSTVNPITIHKRSKVKTEAEGLRPASPLGLTQEQLADLKEDLDRDDCKQEAEVVIYETNCHWADCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHVRAPLLKENGDNEASAEPGGRGPEESVEASSTSHTVEDCLHIKAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGMEMPGTGPGSLGDLTALADTCPGADTSALAAPSTGGLQLRKHMSTVHRFEQLKREKLKSLKDSCSWAGPAPHTRNTKLPPLPVNGSVLENFNNTGGGGPAGLLPSQRLPELTEVTMLSQLQERRDSSTSTMSSAYTVSRRSSGISPYFSSRRSSEASPLGGLRPHNASSADSYDPISTDASRRSSEASQCSGGGPGLLNLTPAQQYNLRAKYAAATGGPPPTPLPGLDRVSLRTRLALLDAPERALPGACPHPLGPRRGSDGPTYSHGHGHGYAGAAPAFPHEGPNSSTRRASDPVRRPDPLILPRVQRFHSTHNMNPGSLPPCADRRGLHVQSHPSVDSNLTRNAYSPRPPSINENVVMEAVAAGVDGPGLECDLGLVEDELVLPDDVVQYIKAHTGGTLDDGIRQGYPTEGTGFPENSKLPSPGLQGHRRLAAADSNMGPSAPGLGGCQLSYSPSSNLNKSNMPVQWNEVSSGTVDALPTQVKPPPFPHSNLAVVQQKPAFGQYPGYNPQSVQSSSGGLDSTQPHLQLRGAPSASRGSYTQQPRQPAAGSQCLGMSAAMSPQASYSQAHPQLSPNIVSGSLNQFSPSCSNMAAKPSHLGLPQQMEVVPNATIMNGHQREHGVPNSSLAAVSQPHPVLSYPQQDSYQQGSNLLSSHQPGFMESQQNAGFGLMQPRPPLEPNTASRHRGVRSGQQQLYARTTGQAMVTSANQETAEAMPKGPAGTMVSLAPQPSQDTGRAQDQNTLYYYGQIHMYEQNGGCPAVQPQPPQPQACSDSIQPEPLPSPGVNQVSSTVDSQLLEPPQIDFDAIMDDGDHSSLFSGALSPTLLHNLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	XM_011247924.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247924.2

ORF Insert Sequence:

ATGAGACACC AGGAGGGAAG GTACCATTAT GACCCTCACT CTGTCCACAG TGTACACGGG 
CCTCCCACCC TAAGTGGCAG CCCTGTCATC TCAGATATCT CCTTGATACG ACTTTCTCCA 
CACCCTGCTG GCCCTGGAGA GTCACCCTTC AGCGCCCACC ACCCCTACGT GAACCCCCAT 
ATGGAGCACT ACCTCCGGTC TGTGCACAGC AGCCCCACAC TCTCAATGAT CTCTGCCGCC 
AGGGGCCTCA GCCCTGCTGA TGTGGCCCAC GAACATCTGA AAGAGAGGGG ACTCTTTAGC 
CTCGCAGCCC CAGGCACCAA CCCTTCAGAC TATTACCACC AGATGACCCT CATGGCAAGC 
CACCCCACCC CTTATGGGGA CCTTCTAATG CAGAGCGGGG GTGCTGCTAG CGCACCCCAT 
CTCCATGACT ACCTCAACCC TGTGGATGCA TCACGATTCT CTAGTCCACG TGTGACCCCA 
CGACTGAGCC GCAAGCGGGC TCTGTCCATC TCCCCGCTCT CAGATGCCAG CCTCGACCTA 
CAACGCATGA TTCGGACCTC TCCCAACTCG CTGGTAGCTT ACATCAACAA CTCCAGGAGC 
AGCTCAGCAG CCAGTGGCTC TTATGGACAT CTGTCTGCTG GTGCCCTCAG CCCAGCCTTC 
ACTTTTCCCC ACCCCATCAA TCCTGTGGCC TACCAGCAGA TCCTGAGCCA GCAGCGGGGC 
CTGGGCTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CTTCACCCAC CTTCTTGGCC 
CAGCAGCCCA TGACTCTCAC CTCCATCAGC ACCATGCCTA CCCAACTCAG CAGCAGTAGC 
AGCAACTGTC TAAATGATGC CAACCAGAAC AAGCAGAACA GCGAGTCAGC TGTGAGCAGC 
ACCGTGAACC CCATCACCAT TCATAAGCGG AGCAAGGTCA AGACTGAGGC TGAGGGCCTG 
CGTCCAGCAT CCCCGCTTGG ACTGACACAG GAGCAGCTGG CCGATCTCAA GGAAGACCTG 
GACAGGGATG ACTGTAAGCA GGAGGCCGAG GTGGTCATCT ACGAGACCAA CTGCCACTGG 
GCAGACTGCA CCAAGGAGTA TGACACACAG GAGCAGCTGG TGCATCATAT CAACAATGAA 
CACATCCACG GGGAGAAGAA GGAGTTCGTG TGCCGCTGGC AGGCCTGCAC GAGAGAGCAG 
AAGCCCTTCA AGGCCCAGTA CATGCTGGTT GTTCACATGC GCAGACACAC GGGTGAGAAG 
CCACACAAGT GCACGTTCGA AGGCTGTTCC AAGGCCTACT CTCGCCTGGA GAACCTGAAG 
ACACACCTGC GTTCACACAC AGGAGAGAAG CCATATGTGT GTGAACACGA AGGCTGTAAC 
AAAGCCTTCT CCAATGCCTC AGACCGCGCC AAGCACCAGA ACCGCACTCA CTCCAATGAG 
AAACCCTACA TCTGCAAGAT CCCAGGCTGC ACCAAGAGGT ACACAGACCC CAGCTCACTC 
CGCAAGCATG TGAAGACTGT CCATGGGCCA GACGCCCATG TCACCAAGAA ACAGCGTAAT 
GATGTGCATG TCCGTGCTCC ACTGCTCAAG GAGAATGGGG ATAATGAGGC CAGCGCCGAG 
CCAGGTGGCC GGGGACCTGA GGAGAGTGTG GAGGCCAGTA GCACCAGCCA CACTGTGGAG 
GACTGCCTAC ATATCAAAGC CATCAAGACA GAGAGCTCCG GGCTTTGTCA GTCCAGCCCC 
GGGGCCCAGT CATCCTGCAG CAGCGAGCCC TCTCCCCTGG GCAGTGCCCC CAACAATGAC 
AGTGGCATGG AGATGCCGGG GACAGGGCCT GGGAGTCTGG GAGACCTGAC AGCACTGGCT 
GACACGTGTC CAGGAGCTGA CACCTCAGCC CTGGCTGCAC CCTCCACTGG TGGCCTGCAG 
CTGCGCAAAC ACATGAGCAC CGTGCATCGC TTTGAGCAGC TGAAGAGAGA GAAGCTCAAG 
TCACTGAAGG ATTCCTGCTC GTGGGCCGGC CCAGCTCCAC ACACCCGCAA CACCAAGCTG 
CCTCCCCTTC CAGTCAATGG TTCTGTCCTG GAAAACTTCA ACAATACAGG GGGCGGTGGA 
CCGGCAGGAC TGCTGCCCAG CCAGCGGCTA CCAGAGCTGA CCGAAGTGAC GATGCTGAGC 
CAGCTGCAGG AACGAAGAGA CAGCTCCACC AGCACCATGA GCTCGGCCTA CACTGTGAGC 
CGCCGCTCCT CTGGCATCTC CCCATACTTC TCTAGCCGTC GCTCCAGCGA GGCTTCGCCT 
CTCGGTGGCC TACGCCCGCA CAACGCCAGC TCAGCAGACT CCTATGACCC CATCTCCACA 
GATGCCTCTC GGCGCTCCAG TGAAGCCAGC CAGTGCAGTG GCGGTGGCCC AGGGCTGCTC 
AACCTCACAC CTGCGCAGCA GTACAACCTG CGTGCCAAGT ACGCAGCGGC CACAGGTGGA 
CCACCGCCCA CGCCACTGCC GGGCCTCGAT CGTGTAAGCC TTCGTACCCG CCTGGCCTTG 
CTGGATGCTC CTGAGCGTGC ACTTCCTGGT GCCTGCCCAC ATCCACTGGG GCCACGGCGT 
GGCAGCGATG GGCCTACCTA TAGCCATGGT CATGGCCATG GCTACGCAGG TGCGGCTCCA 
GCATTCCCCC ACGAGGGGCC AAACAGCAGC ACACGGCGGG CCAGCGACCC TGTGCGGCGC 
CCTGACCCCC TTATTCTGCC TCGAGTGCAA CGTTTCCACA GTACCCACAA CATGAATCCA 
GGTTCACTGC CACCCTGCGC TGATCGGCGT GGCCTGCACG TACAGAGCCA CCCCAGCGTA 
GACAGCAACC TGACCCGCAA CGCCTACTCT CCCAGACCCC CTAGCATCAA TGAGAACGTG 
GTGATGGAGG CCGTGGCTGC TGGGGTAGAC GGCCCAGGGC TAGAGTGCGA CCTGGGGCTG 
GTGGAGGATG AGCTGGTGCT GCCAGATGAT GTGGTACAGT ACATCAAGGC TCACACCGGT 
GGTACCTTGG ATGACGGCAT TCGGCAGGGG TATCCCACAG AAGGTACTGG CTTCCCCGAG 
AACTCTAAGC TGCCCAGTCC TGGGCTACAA GGCCACCGCA GGCTAGCAGC TGCCGACTCC 
AACATGGGTC CTTCTGCTCC TGGACTCGGG GGCTGCCAGC TGAGCTACAG CCCCTCCTCC 
AACCTCAACA AGAGCAACAT GCCTGTGCAG TGGAATGAGG TGAGTTCTGG CACCGTGGAT 
GCCCTGCCTA CCCAGGTGAA GCCACCTCCT TTCCCTCACA GCAACCTGGC TGTGGTCCAA 
CAGAAGCCAG CCTTTGGCCA GTATCCAGGA TATAATCCAC AATCCGTGCA GAGCAGCTCC 
GGAGGTCTAG ACAGCACCCA GCCGCACCTA CAGCTTCGAG GAGCCCCCTC TGCATCAAGA 
GGGAGCTACA CGCAACAGCC TCGACAGCCA GCTGCAGGCA GTCAGTGCCT GGGTATGAGT 
GCGGCCATGA GCCCGCAGGC CAGCTACAGC CAAGCCCACC CCCAGCTGAG CCCAAACATT 
GTCAGCGGAT CTCTGAACCA GTTTTCTCCC TCCTGCAGCA ATATGGCAGC CAAGCCCAGC 
CACCTGGGAC TCCCTCAGCA AATGGAAGTT GTCCCCAATG CCACCATCAT GAATGGCCAT 
CAACGGGAGC ACGGGGTCCC CAATTCATCC CTGGCTGCGG TGTCACAACC TCACCCAGTC 
CTGAGCTATC CCCAGCAGGA CAGCTACCAA CAGGGCTCCA ACCTTCTGTC ATCCCATCAG 
CCTGGCTTCA TGGAGTCCCA GCAGAACGCG GGCTTTGGTC TCATGCAGCC TCGGCCACCC 
CTGGAACCCA ACACGGCCAG CCGTCACCGT GGAGTACGTT CTGGGCAACA GCAGTTGTAT 
GCCAGGACCA CTGGCCAAGC CATGGTCACA TCAGCCAACC AAGAGACAGC AGAAGCTATG 
CCCAAGGGAC CAGCAGGGAC CATGGTATCC CTAGCTCCTC AGCCATCTCA GGACACAGGG 
CGGGCACAAG ATCAGAACAC GCTATACTAC TATGGCCAGA TCCACATGTA TGAACAGAAT 
GGAGGCTGCC CAGCCGTGCA GCCCCAGCCG CCACAACCAC AAGCTTGCTC AGACAGTATC 
CAGCCTGAGC CTTTGCCTTC ACCGGGAGTC AACCAGGTGT CTAGCACCGT GGACTCCCAG 
CTCCTGGAGC CCCCCCAGAT TGACTTTGAT GCCATCATGG ATGATGGTGA TCACTCGAGT 
TTGTTTTCTG GTGCACTGAG CCCAACCCTT CTCCACAATC TCTCCCAGAA TTCCTCACGC 
CTCACCACAC CCCGGAATTC CTTGACACTG CCCTCCATCC CTGCGGGCAT CAGCAACATG 
GCCGTGGGCG ACATGAGTTC CATGCTCACC AGCCTGGCTG AAGAGAGCAA GTTTTTAAAC 
ATGATGACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gli2 ( XM_006529141.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529204.1 Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu27904
Clone ID Related Accession (Same CDS sequence)	XM_011247924.2 , XM_006529141.3 , XM_006529141.4 , XM_011247924.3
Accession Version	XM_006529141.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	zinc finger protein GLI2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529141.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACACC AGGAGGGAAG GTACCATTAT GACCCTCACT CTGTCCACAG TGTACACGGG 
CCTCCCACCC TAAGTGGCAG CCCTGTCATC TCAGATATCT CCTTGATACG ACTTTCTCCA 
CACCCTGCTG GCCCTGGAGA GTCACCCTTC AGCGCCCACC ACCCCTACGT GAACCCCCAT 
ATGGAGCACT ACCTCCGGTC TGTGCACAGC AGCCCCACAC TCTCAATGAT CTCTGCCGCC 
AGGGGCCTCA GCCCTGCTGA TGTGGCCCAC GAACATCTGA AAGAGAGGGG ACTCTTTAGC 
CTCGCAGCCC CAGGCACCAA CCCTTCAGAC TATTACCACC AGATGACCCT CATGGCAAGC 
CACCCCACCC CTTATGGGGA CCTTCTAATG CAGAGCGGGG GTGCTGCTAG CGCACCCCAT 
CTCCATGACT ACCTCAACCC TGTGGATGCA TCACGATTCT CTAGTCCACG TGTGACCCCA 
CGACTGAGCC GCAAGCGGGC TCTGTCCATC TCCCCGCTCT CAGATGCCAG CCTCGACCTA 
CAACGCATGA TTCGGACCTC TCCCAACTCG CTGGTAGCTT ACATCAACAA CTCCAGGAGC 
AGCTCAGCAG CCAGTGGCTC TTATGGACAT CTGTCTGCTG GTGCCCTCAG CCCAGCCTTC 
ACTTTTCCCC ACCCCATCAA TCCTGTGGCC TACCAGCAGA TCCTGAGCCA GCAGCGGGGC 
CTGGGCTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CTTCACCCAC CTTCTTGGCC 
CAGCAGCCCA TGACTCTCAC CTCCATCAGC ACCATGCCTA CCCAACTCAG CAGCAGTAGC 
AGCAACTGTC TAAATGATGC CAACCAGAAC AAGCAGAACA GCGAGTCAGC TGTGAGCAGC 
ACCGTGAACC CCATCACCAT TCATAAGCGG AGCAAGGTCA AGACTGAGGC TGAGGGCCTG 
CGTCCAGCAT CCCCGCTTGG ACTGACACAG GAGCAGCTGG CCGATCTCAA GGAAGACCTG 
GACAGGGATG ACTGTAAGCA GGAGGCCGAG GTGGTCATCT ACGAGACCAA CTGCCACTGG 
GCAGACTGCA CCAAGGAGTA TGACACACAG GAGCAGCTGG TGCATCATAT CAACAATGAA 
CACATCCACG GGGAGAAGAA GGAGTTCGTG TGCCGCTGGC AGGCCTGCAC GAGAGAGCAG 
AAGCCCTTCA AGGCCCAGTA CATGCTGGTT GTTCACATGC GCAGACACAC GGGTGAGAAG 
CCACACAAGT GCACGTTCGA AGGCTGTTCC AAGGCCTACT CTCGCCTGGA GAACCTGAAG 
ACACACCTGC GTTCACACAC AGGAGAGAAG CCATATGTGT GTGAACACGA AGGCTGTAAC 
AAAGCCTTCT CCAATGCCTC AGACCGCGCC AAGCACCAGA ACCGCACTCA CTCCAATGAG 
AAACCCTACA TCTGCAAGAT CCCAGGCTGC ACCAAGAGGT ACACAGACCC CAGCTCACTC 
CGCAAGCATG TGAAGACTGT CCATGGGCCA GACGCCCATG TCACCAAGAA ACAGCGTAAT 
GATGTGCATG TCCGTGCTCC ACTGCTCAAG GAGAATGGGG ATAATGAGGC CAGCGCCGAG 
CCAGGTGGCC GGGGACCTGA GGAGAGTGTG GAGGCCAGTA GCACCAGCCA CACTGTGGAG 
GACTGCCTAC ATATCAAAGC CATCAAGACA GAGAGCTCCG GGCTTTGTCA GTCCAGCCCC 
GGGGCCCAGT CATCCTGCAG CAGCGAGCCC TCTCCCCTGG GCAGTGCCCC CAACAATGAC 
AGTGGCATGG AGATGCCGGG GACAGGGCCT GGGAGTCTGG GAGACCTGAC AGCACTGGCT 
GACACGTGTC CAGGAGCTGA CACCTCAGCC CTGGCTGCAC CCTCCACTGG TGGCCTGCAG 
CTGCGCAAAC ACATGAGCAC CGTGCATCGC TTTGAGCAGC TGAAGAGAGA GAAGCTCAAG 
TCACTGAAGG ATTCCTGCTC GTGGGCCGGC CCAGCTCCAC ACACCCGCAA CACCAAGCTG 
CCTCCCCTTC CAGTCAATGG TTCTGTCCTG GAAAACTTCA ACAATACAGG GGGCGGTGGA 
CCGGCAGGAC TGCTGCCCAG CCAGCGGCTA CCAGAGCTGA CCGAAGTGAC GATGCTGAGC 
CAGCTGCAGG AACGAAGAGA CAGCTCCACC AGCACCATGA GCTCGGCCTA CACTGTGAGC 
CGCCGCTCCT CTGGCATCTC CCCATACTTC TCTAGCCGTC GCTCCAGCGA GGCTTCGCCT 
CTCGGTGGCC TACGCCCGCA CAACGCCAGC TCAGCAGACT CCTATGACCC CATCTCCACA 
GATGCCTCTC GGCGCTCCAG TGAAGCCAGC CAGTGCAGTG GCGGTGGCCC AGGGCTGCTC 
AACCTCACAC CTGCGCAGCA GTACAACCTG CGTGCCAAGT ACGCAGCGGC CACAGGTGGA 
CCACCGCCCA CGCCACTGCC GGGCCTCGAT CGTGTAAGCC TTCGTACCCG CCTGGCCTTG 
CTGGATGCTC CTGAGCGTGC ACTTCCTGGT GCCTGCCCAC ATCCACTGGG GCCACGGCGT 
GGCAGCGATG GGCCTACCTA TAGCCATGGT CATGGCCATG GCTACGCAGG TGCGGCTCCA 
GCATTCCCCC ACGAGGGGCC AAACAGCAGC ACACGGCGGG CCAGCGACCC TGTGCGGCGC 
CCTGACCCCC TTATTCTGCC TCGAGTGCAA CGTTTCCACA GTACCCACAA CATGAATCCA 
GGTTCACTGC CACCCTGCGC TGATCGGCGT GGCCTGCACG TACAGAGCCA CCCCAGCGTA 
GACAGCAACC TGACCCGCAA CGCCTACTCT CCCAGACCCC CTAGCATCAA TGAGAACGTG 
GTGATGGAGG CCGTGGCTGC TGGGGTAGAC GGCCCAGGGC TAGAGTGCGA CCTGGGGCTG 
GTGGAGGATG AGCTGGTGCT GCCAGATGAT GTGGTACAGT ACATCAAGGC TCACACCGGT 
GGTACCTTGG ATGACGGCAT TCGGCAGGGG TATCCCACAG AAGGTACTGG CTTCCCCGAG 
AACTCTAAGC TGCCCAGTCC TGGGCTACAA GGCCACCGCA GGCTAGCAGC TGCCGACTCC 
AACATGGGTC CTTCTGCTCC TGGACTCGGG GGCTGCCAGC TGAGCTACAG CCCCTCCTCC 
AACCTCAACA AGAGCAACAT GCCTGTGCAG TGGAATGAGG TGAGTTCTGG CACCGTGGAT 
GCCCTGCCTA CCCAGGTGAA GCCACCTCCT TTCCCTCACA GCAACCTGGC TGTGGTCCAA 
CAGAAGCCAG CCTTTGGCCA GTATCCAGGA TATAATCCAC AATCCGTGCA GAGCAGCTCC 
GGAGGTCTAG ACAGCACCCA GCCGCACCTA CAGCTTCGAG GAGCCCCCTC TGCATCAAGA 
GGGAGCTACA CGCAACAGCC TCGACAGCCA GCTGCAGGCA GTCAGTGCCT GGGTATGAGT 
GCGGCCATGA GCCCGCAGGC CAGCTACAGC CAAGCCCACC CCCAGCTGAG CCCAAACATT 
GTCAGCGGAT CTCTGAACCA GTTTTCTCCC TCCTGCAGCA ATATGGCAGC CAAGCCCAGC 
CACCTGGGAC TCCCTCAGCA AATGGAAGTT GTCCCCAATG CCACCATCAT GAATGGCCAT 
CAACGGGAGC ACGGGGTCCC CAATTCATCC CTGGCTGCGG TGTCACAACC TCACCCAGTC 
CTGAGCTATC CCCAGCAGGA CAGCTACCAA CAGGGCTCCA ACCTTCTGTC ATCCCATCAG 
CCTGGCTTCA TGGAGTCCCA GCAGAACGCG GGCTTTGGTC TCATGCAGCC TCGGCCACCC 
CTGGAACCCA ACACGGCCAG CCGTCACCGT GGAGTACGTT CTGGGCAACA GCAGTTGTAT 
GCCAGGACCA CTGGCCAAGC CATGGTCACA TCAGCCAACC AAGAGACAGC AGAAGCTATG 
CCCAAGGGAC CAGCAGGGAC CATGGTATCC CTAGCTCCTC AGCCATCTCA GGACACAGGG 
CGGGCACAAG ATCAGAACAC GCTATACTAC TATGGCCAGA TCCACATGTA TGAACAGAAT 
GGAGGCTGCC CAGCCGTGCA GCCCCAGCCG CCACAACCAC AAGCTTGCTC AGACAGTATC 
CAGCCTGAGC CTTTGCCTTC ACCGGGAGTC AACCAGGTGT CTAGCACCGT GGACTCCCAG 
CTCCTGGAGC CCCCCCAGAT TGACTTTGAT GCCATCATGG ATGATGGTGA TCACTCGAGT 
TTGTTTTCTG GTGCACTGAG CCCAACCCTT CTCCACAATC TCTCCCAGAA TTCCTCACGC 
CTCACCACAC CCCGGAATTC CTTGACACTG CCCTCCATCC CTGCGGGCAT CAGCAACATG 
GCCGTGGGCG ACATGAGTTC CATGCTCACC AGCCTGGCTG AAGAGAGCAA GTTTTTAAAC 
ATGATGACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529204.1
CDS	361..4812
Translation	MRHQEGRYHYDPHSVHSVHGPPTLSGSPVISDISLIRLSPHPAGPGESPFSAHHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAHEHLKERGLFSLAAPGTNPSDYYHQMTLMASHPTPYGDLLMQSGGAASAPHLHDYLNPVDASRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMTLTSISTMPTQLSSSSSNCLNDANQNKQNSESAVSSTVNPITIHKRSKVKTEAEGLRPASPLGLTQEQLADLKEDLDRDDCKQEAEVVIYETNCHWADCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHVRAPLLKENGDNEASAEPGGRGPEESVEASSTSHTVEDCLHIKAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGMEMPGTGPGSLGDLTALADTCPGADTSALAAPSTGGLQLRKHMSTVHRFEQLKREKLKSLKDSCSWAGPAPHTRNTKLPPLPVNGSVLENFNNTGGGGPAGLLPSQRLPELTEVTMLSQLQERRDSSTSTMSSAYTVSRRSSGISPYFSSRRSSEASPLGGLRPHNASSADSYDPISTDASRRSSEASQCSGGGPGLLNLTPAQQYNLRAKYAAATGGPPPTPLPGLDRVSLRTRLALLDAPERALPGACPHPLGPRRGSDGPTYSHGHGHGYAGAAPAFPHEGPNSSTRRASDPVRRPDPLILPRVQRFHSTHNMNPGSLPPCADRRGLHVQSHPSVDSNLTRNAYSPRPPSINENVVMEAVAAGVDGPGLECDLGLVEDELVLPDDVVQYIKAHTGGTLDDGIRQGYPTEGTGFPENSKLPSPGLQGHRRLAAADSNMGPSAPGLGGCQLSYSPSSNLNKSNMPVQWNEVSSGTVDALPTQVKPPPFPHSNLAVVQQKPAFGQYPGYNPQSVQSSSGGLDSTQPHLQLRGAPSASRGSYTQQPRQPAAGSQCLGMSAAMSPQASYSQAHPQLSPNIVSGSLNQFSPSCSNMAAKPSHLGLPQQMEVVPNATIMNGHQREHGVPNSSLAAVSQPHPVLSYPQQDSYQQGSNLLSSHQPGFMESQQNAGFGLMQPRPPLEPNTASRHRGVRSGQQQLYARTTGQAMVTSANQETAEAMPKGPAGTMVSLAPQPSQDTGRAQDQNTLYYYGQIHMYEQNGGCPAVQPQPPQPQACSDSIQPEPLPSPGVNQVSSTVDSQLLEPPQIDFDAIMDDGDHSSLFSGALSPTLLHNLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	XM_006529141.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529141.3

ORF Insert Sequence:

ATGAGACACC AGGAGGGAAG GTACCATTAT GACCCTCACT CTGTCCACAG TGTACACGGG 
CCTCCCACCC TAAGTGGCAG CCCTGTCATC TCAGATATCT CCTTGATACG ACTTTCTCCA 
CACCCTGCTG GCCCTGGAGA GTCACCCTTC AGCGCCCACC ACCCCTACGT GAACCCCCAT 
ATGGAGCACT ACCTCCGGTC TGTGCACAGC AGCCCCACAC TCTCAATGAT CTCTGCCGCC 
AGGGGCCTCA GCCCTGCTGA TGTGGCCCAC GAACATCTGA AAGAGAGGGG ACTCTTTAGC 
CTCGCAGCCC CAGGCACCAA CCCTTCAGAC TATTACCACC AGATGACCCT CATGGCAAGC 
CACCCCACCC CTTATGGGGA CCTTCTAATG CAGAGCGGGG GTGCTGCTAG CGCACCCCAT 
CTCCATGACT ACCTCAACCC TGTGGATGCA TCACGATTCT CTAGTCCACG TGTGACCCCA 
CGACTGAGCC GCAAGCGGGC TCTGTCCATC TCCCCGCTCT CAGATGCCAG CCTCGACCTA 
CAACGCATGA TTCGGACCTC TCCCAACTCG CTGGTAGCTT ACATCAACAA CTCCAGGAGC 
AGCTCAGCAG CCAGTGGCTC TTATGGACAT CTGTCTGCTG GTGCCCTCAG CCCAGCCTTC 
ACTTTTCCCC ACCCCATCAA TCCTGTGGCC TACCAGCAGA TCCTGAGCCA GCAGCGGGGC 
CTGGGCTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CTTCACCCAC CTTCTTGGCC 
CAGCAGCCCA TGACTCTCAC CTCCATCAGC ACCATGCCTA CCCAACTCAG CAGCAGTAGC 
AGCAACTGTC TAAATGATGC CAACCAGAAC AAGCAGAACA GCGAGTCAGC TGTGAGCAGC 
ACCGTGAACC CCATCACCAT TCATAAGCGG AGCAAGGTCA AGACTGAGGC TGAGGGCCTG 
CGTCCAGCAT CCCCGCTTGG ACTGACACAG GAGCAGCTGG CCGATCTCAA GGAAGACCTG 
GACAGGGATG ACTGTAAGCA GGAGGCCGAG GTGGTCATCT ACGAGACCAA CTGCCACTGG 
GCAGACTGCA CCAAGGAGTA TGACACACAG GAGCAGCTGG TGCATCATAT CAACAATGAA 
CACATCCACG GGGAGAAGAA GGAGTTCGTG TGCCGCTGGC AGGCCTGCAC GAGAGAGCAG 
AAGCCCTTCA AGGCCCAGTA CATGCTGGTT GTTCACATGC GCAGACACAC GGGTGAGAAG 
CCACACAAGT GCACGTTCGA AGGCTGTTCC AAGGCCTACT CTCGCCTGGA GAACCTGAAG 
ACACACCTGC GTTCACACAC AGGAGAGAAG CCATATGTGT GTGAACACGA AGGCTGTAAC 
AAAGCCTTCT CCAATGCCTC AGACCGCGCC AAGCACCAGA ACCGCACTCA CTCCAATGAG 
AAACCCTACA TCTGCAAGAT CCCAGGCTGC ACCAAGAGGT ACACAGACCC CAGCTCACTC 
CGCAAGCATG TGAAGACTGT CCATGGGCCA GACGCCCATG TCACCAAGAA ACAGCGTAAT 
GATGTGCATG TCCGTGCTCC ACTGCTCAAG GAGAATGGGG ATAATGAGGC CAGCGCCGAG 
CCAGGTGGCC GGGGACCTGA GGAGAGTGTG GAGGCCAGTA GCACCAGCCA CACTGTGGAG 
GACTGCCTAC ATATCAAAGC CATCAAGACA GAGAGCTCCG GGCTTTGTCA GTCCAGCCCC 
GGGGCCCAGT CATCCTGCAG CAGCGAGCCC TCTCCCCTGG GCAGTGCCCC CAACAATGAC 
AGTGGCATGG AGATGCCGGG GACAGGGCCT GGGAGTCTGG GAGACCTGAC AGCACTGGCT 
GACACGTGTC CAGGAGCTGA CACCTCAGCC CTGGCTGCAC CCTCCACTGG TGGCCTGCAG 
CTGCGCAAAC ACATGAGCAC CGTGCATCGC TTTGAGCAGC TGAAGAGAGA GAAGCTCAAG 
TCACTGAAGG ATTCCTGCTC GTGGGCCGGC CCAGCTCCAC ACACCCGCAA CACCAAGCTG 
CCTCCCCTTC CAGTCAATGG TTCTGTCCTG GAAAACTTCA ACAATACAGG GGGCGGTGGA 
CCGGCAGGAC TGCTGCCCAG CCAGCGGCTA CCAGAGCTGA CCGAAGTGAC GATGCTGAGC 
CAGCTGCAGG AACGAAGAGA CAGCTCCACC AGCACCATGA GCTCGGCCTA CACTGTGAGC 
CGCCGCTCCT CTGGCATCTC CCCATACTTC TCTAGCCGTC GCTCCAGCGA GGCTTCGCCT 
CTCGGTGGCC TACGCCCGCA CAACGCCAGC TCAGCAGACT CCTATGACCC CATCTCCACA 
GATGCCTCTC GGCGCTCCAG TGAAGCCAGC CAGTGCAGTG GCGGTGGCCC AGGGCTGCTC 
AACCTCACAC CTGCGCAGCA GTACAACCTG CGTGCCAAGT ACGCAGCGGC CACAGGTGGA 
CCACCGCCCA CGCCACTGCC GGGCCTCGAT CGTGTAAGCC TTCGTACCCG CCTGGCCTTG 
CTGGATGCTC CTGAGCGTGC ACTTCCTGGT GCCTGCCCAC ATCCACTGGG GCCACGGCGT 
GGCAGCGATG GGCCTACCTA TAGCCATGGT CATGGCCATG GCTACGCAGG TGCGGCTCCA 
GCATTCCCCC ACGAGGGGCC AAACAGCAGC ACACGGCGGG CCAGCGACCC TGTGCGGCGC 
CCTGACCCCC TTATTCTGCC TCGAGTGCAA CGTTTCCACA GTACCCACAA CATGAATCCA 
GGTTCACTGC CACCCTGCGC TGATCGGCGT GGCCTGCACG TACAGAGCCA CCCCAGCGTA 
GACAGCAACC TGACCCGCAA CGCCTACTCT CCCAGACCCC CTAGCATCAA TGAGAACGTG 
GTGATGGAGG CCGTGGCTGC TGGGGTAGAC GGCCCAGGGC TAGAGTGCGA CCTGGGGCTG 
GTGGAGGATG AGCTGGTGCT GCCAGATGAT GTGGTACAGT ACATCAAGGC TCACACCGGT 
GGTACCTTGG ATGACGGCAT TCGGCAGGGG TATCCCACAG AAGGTACTGG CTTCCCCGAG 
AACTCTAAGC TGCCCAGTCC TGGGCTACAA GGCCACCGCA GGCTAGCAGC TGCCGACTCC 
AACATGGGTC CTTCTGCTCC TGGACTCGGG GGCTGCCAGC TGAGCTACAG CCCCTCCTCC 
AACCTCAACA AGAGCAACAT GCCTGTGCAG TGGAATGAGG TGAGTTCTGG CACCGTGGAT 
GCCCTGCCTA CCCAGGTGAA GCCACCTCCT TTCCCTCACA GCAACCTGGC TGTGGTCCAA 
CAGAAGCCAG CCTTTGGCCA GTATCCAGGA TATAATCCAC AATCCGTGCA GAGCAGCTCC 
GGAGGTCTAG ACAGCACCCA GCCGCACCTA CAGCTTCGAG GAGCCCCCTC TGCATCAAGA 
GGGAGCTACA CGCAACAGCC TCGACAGCCA GCTGCAGGCA GTCAGTGCCT GGGTATGAGT 
GCGGCCATGA GCCCGCAGGC CAGCTACAGC CAAGCCCACC CCCAGCTGAG CCCAAACATT 
GTCAGCGGAT CTCTGAACCA GTTTTCTCCC TCCTGCAGCA ATATGGCAGC CAAGCCCAGC 
CACCTGGGAC TCCCTCAGCA AATGGAAGTT GTCCCCAATG CCACCATCAT GAATGGCCAT 
CAACGGGAGC ACGGGGTCCC CAATTCATCC CTGGCTGCGG TGTCACAACC TCACCCAGTC 
CTGAGCTATC CCCAGCAGGA CAGCTACCAA CAGGGCTCCA ACCTTCTGTC ATCCCATCAG 
CCTGGCTTCA TGGAGTCCCA GCAGAACGCG GGCTTTGGTC TCATGCAGCC TCGGCCACCC 
CTGGAACCCA ACACGGCCAG CCGTCACCGT GGAGTACGTT CTGGGCAACA GCAGTTGTAT 
GCCAGGACCA CTGGCCAAGC CATGGTCACA TCAGCCAACC AAGAGACAGC AGAAGCTATG 
CCCAAGGGAC CAGCAGGGAC CATGGTATCC CTAGCTCCTC AGCCATCTCA GGACACAGGG 
CGGGCACAAG ATCAGAACAC GCTATACTAC TATGGCCAGA TCCACATGTA TGAACAGAAT 
GGAGGCTGCC CAGCCGTGCA GCCCCAGCCG CCACAACCAC AAGCTTGCTC AGACAGTATC 
CAGCCTGAGC CTTTGCCTTC ACCGGGAGTC AACCAGGTGT CTAGCACCGT GGACTCCCAG 
CTCCTGGAGC CCCCCCAGAT TGACTTTGAT GCCATCATGG ATGATGGTGA TCACTCGAGT 
TTGTTTTCTG GTGCACTGAG CCCAACCCTT CTCCACAATC TCTCCCAGAA TTCCTCACGC 
CTCACCACAC CCCGGAATTC CTTGACACTG CCCTCCATCC CTGCGGGCAT CAGCAACATG 
GCCGTGGGCG ACATGAGTTC CATGCTCACC AGCCTGGCTG AAGAGAGCAA GTTTTTAAAC 
ATGATGACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Gli2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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