GLI2 cDNA ORF clone, Bos taurus(cattle)

The following GLI2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLI2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa37605 NM_001192250.2
Latest version!
Bos taurus GLI family zinc finger 2 (GLI2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa87936 XM_015458080.2
Latest version!
Bos taurus GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa87937 XM_015458081.1
Latest version!
Bos taurus GLI family zinc finger 2 (GLI2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OBa87936 XM_015458080.1 Bos taurus GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa37605
    Clone ID Related Accession (Same CDS sequence) NM_001192250.2
    Accession Version NM_001192250.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product zinc finger protein GLI2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000002.1. On Feb 13, 2012 this sequence version replaced NM_001192250.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145422, SAMN03145425 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGACAT CCGCCTCAGC CACGGCCACG GACAAGAAAG AAGTCAAAGG TGGGATCCTG 
    GAGGGCCTTG CTTTCCCTGA CCCCGGCAGG AAAGCCTCGG CCCTCGCGGT GGCCACGGCG
    GCGGCTGCGG CCGCAGTAGC TGCCCAAGGA GTGCCGCCGC ATCTCCTGCC ACCGTTCCAC
    GCCCCCTTCC CGATTGACAT GCGACACCAG GAGGGACGGT ACCATTACGA GCCTCACTCT
    GTCCCCGGCG TGCACGGGCC TCCCGCCCTG AGCGGCAGCC CTGTCATCTC TGATATCTCA
    TTGATTCGGC TTTCCCCACA CCCTGCCGGC CCTGGGGAGT CCCCCTTCAA CGCCCCCCAC
    CCGTACGTGA GCCCCCACAT GGACCATTAC CTGCGGGCTG TGCACGGCAG CCCAACACAT
    CCCGCCATCT CTGCAGCCAG GGGCCTCAGC CCCGCGGATG TGGCTCACGA GCACCTTAAG
    GAGCGGGGGC TCTTTGGCCT TCCCGCTGCA GGCACCAACG CCTCAGACTA TTACCACCAG
    ATGACTCTCA TGGCAGGTCA CCCCGCGCCA TATGGGGACC TGCTGATGCA GACTGGGGGT
    GCGGCCAGTG CACCCCATCT CCATGACTAC CTCAATCCAG TGGATGTGTC ACGTTTCTCC
    AGCCCCCGGG TGACACCCCG CCTGAGCCGC AAGCGGGCGC TGTCCATCTC CCCGCTCTCA
    GACGCCAGCC TCGACCTCCA GCGCATGATC CGGACCTCGC CCAACTCGCT GGTGGCCTAC
    ATCAACAACT CCCGCAGCAG CTCGGCTGCC AGCGGCTCCT ACGGACACCT GTCGGCCGGT
    GCCCTCAGCC CAGCCTTCAC CTTCCCCCAC CCCATCAACC CCGTGGCCTA CCAGCAGATC
    CTGAGCCAGC AGAGGGGCCT GGGCTCAGCC TTCGGACACA CGCCACCCCT GATCCAGCCC
    TCGCCCACCT TCCTGGCCCA GCAGCCAGTG GCCTTCACCT CCATCAATGC CACACCCACC
    CAGCTCAGTA GCAGCAGCAA CTGTCTGAGT GACGCCAATC AGAACAAGCA GAGCAGCGAG
    TCAGCCGTGA GCAGCACTGT CAACCCCATC ATCATTCACA AGCGCAGCAA GGTCAAAACT
    GAGGCTGACG GCCTACGGCC AGCCTCCCCA CTGACCCTAA CACAGGTAAC CTGCCAGCTG
    TCCTCGGGCC GGGCTCCAGC CTTGTCCCCT AGGGGCCGTG GCCGCACAAG GCTCACGTGG
    GTATGCTCTG CCCCACTCCC CCAGGAGCAG CTGGCTGACC TCAAGGAGGA CCTGGAGAGA
    GACGATTGTA AGCAGGAGGC TGAGGTGGTC ATCTATGAGA CCAACTGCCA CTGGGAGGAC
    TGCACCAAGG AGTACGACAC GCAGGAGCAG CTGGTGCACC ACATCAACAA CGAGCACATC
    CACGGGGAGA AGAAGGAGTT TGTGTGCCGC TGGCAGGCCT GCACGCGTGA GCAGAAGCCC
    TTCAAGGCAC AGTACATGCT TGTGGTGCAC ATGCGCAGAC ACACAGGCGA GAAGCCCCAC
    AAGTGCACGT TCGAGGGCTG CTCAAAGGCC TATTCCCGCC TGGAGAACCT GAAGACTCAC
    CTGCGCTCCC ACACCGGGGA AAAGCCGTAT GTGTGTGAGC ACGAGGGCTG CAACAAAGCC
    TTCTCCAATG CCTCAGACCG CGCCAAGCAC CAGAACCGCA CCCACTCCAA CGAGAAACCC
    TACATCTGCA AGATTCCAGG CTGCACCAAG CGGTACACGG ACCCCAGCTC CCTCCGGAAG
    CACGTGAAGA CGGTCCATGG CCCAGACGCT CACGTCACCA AGAAACAGCG CAACGATGTG
    CACCTCCGCG CCCCTCTGCT CCGGGAGAAT GGGGACAGCG AGGCCGGTGC CGAGCCCGGC
    CGGGGCTCCG AGGACAACGC CGAGGCCAGC AGCACTAGCC AGGCCGTGGA GGACGGCCTG
    CACGTCAAGG CCATCAAGAC GGAGCGCTCC GGGCTGTGTC AGTCCAGCCC CGGAGCCCAG
    TCTTCCTGCA GCAGCGAGCC CTCTCCCCTG GGCAGTGCCC CCAACAATGA CAGTGGCGTG
    GAGATGCCGG GGACAGGGCC CGGGAGCCTG GCAGACCTGA CAGCTCTGGA GGACCCGCCG
    CCAGGGGCTG ACACCTCCGC CCTGGCCGCC CCCTCTGCTG GTGGCCTACA GCTGCGCAAA
    CACATGACCG CTATGCACCG GTTCGAGCAG CTCAAGAAGG AGAAACTCAA GTCACTGAAG
    GATTCCTGCT CATGGGCCGG GCCAGCTCCG CACACCCGGA CCTCCAAGCT GCCTCCCCTC
    CCGGGAAGTG GCTCCGTGCT GGAAAACTTC AGCGGCGGCG GGGGCGGCGG ACCCGCGGGG
    CTGCTGCCCA ACCCGCGGCT GTCTGAGCTG TTCCCGGGCG AGGTGACAGT GCTGAGCCAC
    CTGCAGGAAC GCCGGGACAG CTCCACCAGC ACAGTCAGCT CGGCCTACAC CGTGAGCCGC
    CGCTCCTCCG GCATCTCCCC CTACTTCTCT AGTCGCCGCT CCAGCGAGGC CTCGCCGCTG
    GGCGCCGGCC GCGCGCACCC TGCCAGCTCT GCTGATTCCT ACGACCCCAT CTCCACCGAC
    GCATCACGGC GCTCGAGTGA GGCCAGTCAG TGCGGCGGCG GCCCGGGGCT TCTCCAGCTC
    ACACCCGCCC AGCAGTACAG CCTACGGGCC AAGTACGCAG CAGCCACAGG TGGACCTCCC
    CCGACCCCGC TGCCGGGCCT GGAGCGCACG AGCCTGCGGA CCCGGCTGGC GCTGCTGGAT
    GCTCCCGAGC GCGCGCTGCC CGCTGCCTGC CTGCGCCCGA TCGGGCCTCG GCGGGGCAGC
    GACGGGCCAC ACTACGGGCC CTCGGGGCCG TTGCCCGCTT TCCCCCACGA GGCACCAGGC
    GGCGGGGCGC GGCGGGCCAG CGATCCTGGG AGGCGGCCTG ATGCCCTGGC AGCCCCGCGG
    GTGCAGCGCT TCCACAGCGC CCTCGACGTG ACCCCAGGCA CGCTACCGCC ACCCTGCGGC
    CAGGGCCGAG GCCTCCGTCT GCAAAGCCAC ACGAGCGCAG ACGGCAGCCT GGCCCGCAGC
    GGCTACTCGC CCCGGCCGCC CAGCATCAGC GAGAACGTGG TGATGGAAGC CTTGGCGGCG
    GGGGCGGACG GCACAGGCCC AGAGGTCAGC CTCGGACTGC CAGAGGACGA CCTCGTGCTC
    CCCGATGACG TGGTGCAGTA CATCAAGGCA CGTACCAGTG GCACCCTGGA GGAGAGTACC
    CCACCGGTGT ACCCCCCGGA AAGCACCTGC TTCTCTGAGA ACCCCAAACT GCCCAGCCCC
    GGGCTGCACG GCCAACGTAC CATAGCAGCT GCAGACTCAA ACGTGGGCCC CTCCGCCCCG
    GTGCTGGGAG GCTGCCCACT GGGCTACGGG GCCCCCTCCA GCCTGAACAA GAACAGCATG
    CCTGTGCAGT GGAACGAAGT GAGCTCAGGC ACCGTGGACG CACTGGCCAG CCAGGTGAAG
    CCTCTGCCCT TCCCACAGGG CAACCTAGCT GTGGTACCAC AGAAACCAGC CTTCGCCCAG
    TACCCAGGAT ACAATCCGCA GGGCCTGCAG CCGAGCCCTG GGGGCCTAGA CAACTCACAG
    CCACACTTAC AGCCCTACAG TGGAGCCCCA TCCGCACCCA GGGGAAATTA CACACAGCAG
    CTGCGGCAGC CAGGGGCTGG CAGCCAGTGT CCCAACATGA CTGCTGCAGT GAACCCCCAC
    CCCGGCTATG GCCAAGCCCA CCCCCAGCTG AGCACCATGG GTGGGACCCT GAACCAATTC
    CCCTCCTCTT GCAGCAACAT GGCAACCAAG CCAGGCCACC TGGGGCTCCC CCAGCAAATG
    GAAGTTGCTC CTGACCCCAC CATGATGGGC AACAGCCACA GGGAGCTCGG CATGCCCAAT
    TCCAGCCTGG CCGGGATGCC ACCACCTCAT CCAGCACAGA ACTACCCACA GCAGAGTCAT
    CACCTGGTGA CCACCATGAG CCAGGAGGGC TACCACCAAG GCCCCAACCT GGTACCTTCC
    CACCAACCTA GTTTCATGGA GCCCCAGCAG GGCACAATGG GGGCAGCTGG ATCTAGCTTT
    GGCCTAATGC AACCCCGGCC ACCCCCAGAG CCTGGCCCTG CCGGCCGCCA CCGTGGGGTA
    CGTGCAGGGC AGCAGCTAGC CTATGCCAGG GCAACTGGCC ATGCCATGGC TGCTATGTCA
    GCCAACCAGG AGATGACAGA GTCTGTACCC AAGGGGACAA TGGGCAGCAT GTTGTCCCTA
    CCCCCTCAGC CACCCCCACA GGACACAGGT GGGACCCAGG ACCACAGCAT GCTCTACTAC
    TATGGCCAGA TCCACATGTA CGAGCAGAAC GGAGGCCCTG AGAACCAGGC CGGCTGCCAA
    GCCCTGCGAC CCCAGCCAAC ACAGCCCCAG GCCTTGCCCT CGCCGGGGGT CAACCAGGTG
    TCCAGCACTG TGGACTCCCA GCTACTTGAG GCACCCCAGA TTGATTTTGA TGCCATCATG
    GATGACAGTG ATCACTCCAG CTTGCTTTCT GGCGCCCTGA GCCCCAGCCT CCTCCACGGC
    CTCTCCCAGA GCTCCTCCCG CCTCACCACC CCACGCAGCT CCCTGACTCT GCCCTCCATC
    CCCGCCGGTA TCAGCAACAT GGCCGTGGGG GACATGAGCT CCATGCTCAC CAGCCTGGCC
    GAGGAGAGCA AGTTCTTGAA CATGATGACC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179179.2
    CDS1..4773
    Translation

    Target ORF information:

    RefSeq Version NM_001192250.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 2 (GLI2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192250.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGACAT CCGCCTCAGC CACGGCCACG GACAAGAAAG AAGTCAAAGG TGGGATCCTG 
    GAGGGCCTTG CTTTCCCTGA CCCCGGCAGG AAAGCCTCGG CCCTCGCGGT GGCCACGGCG
    GCGGCTGCGG CCGCAGTAGC TGCCCAAGGA GTGCCGCCGC ATCTCCTGCC ACCGTTCCAC
    GCCCCCTTCC CGATTGACAT GCGACACCAG GAGGGACGGT ACCATTACGA GCCTCACTCT
    GTCCCCGGCG TGCACGGGCC TCCCGCCCTG AGCGGCAGCC CTGTCATCTC TGATATCTCA
    TTGATTCGGC TTTCCCCACA CCCTGCCGGC CCTGGGGAGT CCCCCTTCAA CGCCCCCCAC
    CCGTACGTGA GCCCCCACAT GGACCATTAC CTGCGGGCTG TGCACGGCAG CCCAACACAT
    CCCGCCATCT CTGCAGCCAG GGGCCTCAGC CCCGCGGATG TGGCTCACGA GCACCTTAAG
    GAGCGGGGGC TCTTTGGCCT TCCCGCTGCA GGCACCAACG CCTCAGACTA TTACCACCAG
    ATGACTCTCA TGGCAGGTCA CCCCGCGCCA TATGGGGACC TGCTGATGCA GACTGGGGGT
    GCGGCCAGTG CACCCCATCT CCATGACTAC CTCAATCCAG TGGATGTGTC ACGTTTCTCC
    AGCCCCCGGG TGACACCCCG CCTGAGCCGC AAGCGGGCGC TGTCCATCTC CCCGCTCTCA
    GACGCCAGCC TCGACCTCCA GCGCATGATC CGGACCTCGC CCAACTCGCT GGTGGCCTAC
    ATCAACAACT CCCGCAGCAG CTCGGCTGCC AGCGGCTCCT ACGGACACCT GTCGGCCGGT
    GCCCTCAGCC CAGCCTTCAC CTTCCCCCAC CCCATCAACC CCGTGGCCTA CCAGCAGATC
    CTGAGCCAGC AGAGGGGCCT GGGCTCAGCC TTCGGACACA CGCCACCCCT GATCCAGCCC
    TCGCCCACCT TCCTGGCCCA GCAGCCAGTG GCCTTCACCT CCATCAATGC CACACCCACC
    CAGCTCAGTA GCAGCAGCAA CTGTCTGAGT GACGCCAATC AGAACAAGCA GAGCAGCGAG
    TCAGCCGTGA GCAGCACTGT CAACCCCATC ATCATTCACA AGCGCAGCAA GGTCAAAACT
    GAGGCTGACG GCCTACGGCC AGCCTCCCCA CTGACCCTAA CACAGGTAAC CTGCCAGCTG
    TCCTCGGGCC GGGCTCCAGC CTTGTCCCCT AGGGGCCGTG GCCGCACAAG GCTCACGTGG
    GTATGCTCTG CCCCACTCCC CCAGGAGCAG CTGGCTGACC TCAAGGAGGA CCTGGAGAGA
    GACGATTGTA AGCAGGAGGC TGAGGTGGTC ATCTATGAGA CCAACTGCCA CTGGGAGGAC
    TGCACCAAGG AGTACGACAC GCAGGAGCAG CTGGTGCACC ACATCAACAA CGAGCACATC
    CACGGGGAGA AGAAGGAGTT TGTGTGCCGC TGGCAGGCCT GCACGCGTGA GCAGAAGCCC
    TTCAAGGCAC AGTACATGCT TGTGGTGCAC ATGCGCAGAC ACACAGGCGA GAAGCCCCAC
    AAGTGCACGT TCGAGGGCTG CTCAAAGGCC TATTCCCGCC TGGAGAACCT GAAGACTCAC
    CTGCGCTCCC ACACCGGGGA AAAGCCGTAT GTGTGTGAGC ACGAGGGCTG CAACAAAGCC
    TTCTCCAATG CCTCAGACCG CGCCAAGCAC CAGAACCGCA CCCACTCCAA CGAGAAACCC
    TACATCTGCA AGATTCCAGG CTGCACCAAG CGGTACACGG ACCCCAGCTC CCTCCGGAAG
    CACGTGAAGA CGGTCCATGG CCCAGACGCT CACGTCACCA AGAAACAGCG CAACGATGTG
    CACCTCCGCG CCCCTCTGCT CCGGGAGAAT GGGGACAGCG AGGCCGGTGC CGAGCCCGGC
    CGGGGCTCCG AGGACAACGC CGAGGCCAGC AGCACTAGCC AGGCCGTGGA GGACGGCCTG
    CACGTCAAGG CCATCAAGAC GGAGCGCTCC GGGCTGTGTC AGTCCAGCCC CGGAGCCCAG
    TCTTCCTGCA GCAGCGAGCC CTCTCCCCTG GGCAGTGCCC CCAACAATGA CAGTGGCGTG
    GAGATGCCGG GGACAGGGCC CGGGAGCCTG GCAGACCTGA CAGCTCTGGA GGACCCGCCG
    CCAGGGGCTG ACACCTCCGC CCTGGCCGCC CCCTCTGCTG GTGGCCTACA GCTGCGCAAA
    CACATGACCG CTATGCACCG GTTCGAGCAG CTCAAGAAGG AGAAACTCAA GTCACTGAAG
    GATTCCTGCT CATGGGCCGG GCCAGCTCCG CACACCCGGA CCTCCAAGCT GCCTCCCCTC
    CCGGGAAGTG GCTCCGTGCT GGAAAACTTC AGCGGCGGCG GGGGCGGCGG ACCCGCGGGG
    CTGCTGCCCA ACCCGCGGCT GTCTGAGCTG TTCCCGGGCG AGGTGACAGT GCTGAGCCAC
    CTGCAGGAAC GCCGGGACAG CTCCACCAGC ACAGTCAGCT CGGCCTACAC CGTGAGCCGC
    CGCTCCTCCG GCATCTCCCC CTACTTCTCT AGTCGCCGCT CCAGCGAGGC CTCGCCGCTG
    GGCGCCGGCC GCGCGCACCC TGCCAGCTCT GCTGATTCCT ACGACCCCAT CTCCACCGAC
    GCATCACGGC GCTCGAGTGA GGCCAGTCAG TGCGGCGGCG GCCCGGGGCT TCTCCAGCTC
    ACACCCGCCC AGCAGTACAG CCTACGGGCC AAGTACGCAG CAGCCACAGG TGGACCTCCC
    CCGACCCCGC TGCCGGGCCT GGAGCGCACG AGCCTGCGGA CCCGGCTGGC GCTGCTGGAT
    GCTCCCGAGC GCGCGCTGCC CGCTGCCTGC CTGCGCCCGA TCGGGCCTCG GCGGGGCAGC
    GACGGGCCAC ACTACGGGCC CTCGGGGCCG TTGCCCGCTT TCCCCCACGA GGCACCAGGC
    GGCGGGGCGC GGCGGGCCAG CGATCCTGGG AGGCGGCCTG ATGCCCTGGC AGCCCCGCGG
    GTGCAGCGCT TCCACAGCGC CCTCGACGTG ACCCCAGGCA CGCTACCGCC ACCCTGCGGC
    CAGGGCCGAG GCCTCCGTCT GCAAAGCCAC ACGAGCGCAG ACGGCAGCCT GGCCCGCAGC
    GGCTACTCGC CCCGGCCGCC CAGCATCAGC GAGAACGTGG TGATGGAAGC CTTGGCGGCG
    GGGGCGGACG GCACAGGCCC AGAGGTCAGC CTCGGACTGC CAGAGGACGA CCTCGTGCTC
    CCCGATGACG TGGTGCAGTA CATCAAGGCA CGTACCAGTG GCACCCTGGA GGAGAGTACC
    CCACCGGTGT ACCCCCCGGA AAGCACCTGC TTCTCTGAGA ACCCCAAACT GCCCAGCCCC
    GGGCTGCACG GCCAACGTAC CATAGCAGCT GCAGACTCAA ACGTGGGCCC CTCCGCCCCG
    GTGCTGGGAG GCTGCCCACT GGGCTACGGG GCCCCCTCCA GCCTGAACAA GAACAGCATG
    CCTGTGCAGT GGAACGAAGT GAGCTCAGGC ACCGTGGACG CACTGGCCAG CCAGGTGAAG
    CCTCTGCCCT TCCCACAGGG CAACCTAGCT GTGGTACCAC AGAAACCAGC CTTCGCCCAG
    TACCCAGGAT ACAATCCGCA GGGCCTGCAG CCGAGCCCTG GGGGCCTAGA CAACTCACAG
    CCACACTTAC AGCCCTACAG TGGAGCCCCA TCCGCACCCA GGGGAAATTA CACACAGCAG
    CTGCGGCAGC CAGGGGCTGG CAGCCAGTGT CCCAACATGA CTGCTGCAGT GAACCCCCAC
    CCCGGCTATG GCCAAGCCCA CCCCCAGCTG AGCACCATGG GTGGGACCCT GAACCAATTC
    CCCTCCTCTT GCAGCAACAT GGCAACCAAG CCAGGCCACC TGGGGCTCCC CCAGCAAATG
    GAAGTTGCTC CTGACCCCAC CATGATGGGC AACAGCCACA GGGAGCTCGG CATGCCCAAT
    TCCAGCCTGG CCGGGATGCC ACCACCTCAT CCAGCACAGA ACTACCCACA GCAGAGTCAT
    CACCTGGTGA CCACCATGAG CCAGGAGGGC TACCACCAAG GCCCCAACCT GGTACCTTCC
    CACCAACCTA GTTTCATGGA GCCCCAGCAG GGCACAATGG GGGCAGCTGG ATCTAGCTTT
    GGCCTAATGC AACCCCGGCC ACCCCCAGAG CCTGGCCCTG CCGGCCGCCA CCGTGGGGTA
    CGTGCAGGGC AGCAGCTAGC CTATGCCAGG GCAACTGGCC ATGCCATGGC TGCTATGTCA
    GCCAACCAGG AGATGACAGA GTCTGTACCC AAGGGGACAA TGGGCAGCAT GTTGTCCCTA
    CCCCCTCAGC CACCCCCACA GGACACAGGT GGGACCCAGG ACCACAGCAT GCTCTACTAC
    TATGGCCAGA TCCACATGTA CGAGCAGAAC GGAGGCCCTG AGAACCAGGC CGGCTGCCAA
    GCCCTGCGAC CCCAGCCAAC ACAGCCCCAG GCCTTGCCCT CGCCGGGGGT CAACCAGGTG
    TCCAGCACTG TGGACTCCCA GCTACTTGAG GCACCCCAGA TTGATTTTGA TGCCATCATG
    GATGACAGTG ATCACTCCAG CTTGCTTTCT GGCGCCCTGA GCCCCAGCCT CCTCCACGGC
    CTCTCCCAGA GCTCCTCCCG CCTCACCACC CCACGCAGCT CCCTGACTCT GCCCTCCATC
    CCCGCCGGTA TCAGCAACAT GGCCGTGGGG GACATGAGCT CCATGCTCAC CAGCCTGGCC
    GAGGAGAGCA AGTTCTTGAA CATGATGACC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87936
    Clone ID Related Accession (Same CDS sequence) XM_015458080.1 , XM_015458080.2
    Accession Version XM_015458080.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product zinc finger protein GLI2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103843.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGACAT CCGCCTCAGC CACGGCCACG GACAAGAAAG AAGTCAAAGG TGGGATCCTG 
    GAGGGCCTTG CTTTCCCTGA CCCCGGCAGG AAAGCCTCGG CCCTCGCGGT GGCCACGGCG
    GCGGCTGCGG CCGCAGTAGC TGCCCAAGGA GTGCCGCCGC ATCTCCTGCC ACCGTTCCAC
    GCCCCCTTCC CGATTGACAT GCGACACCAG GAGGGACGGT ACCATTACGA GCCTCACTCT
    GTCCCCGGCG TGCACGGGCC TCCCGCCCTG AGCGGCAGCC CTGTCATCTC TGATATCTCA
    TTGATTCGGC TTTCCCCACA CCCTGCCGGC CCTGGGGAGT CCCCCTTCAA CGCCCCCCAC
    CCGTACGTGA GCCCCCACAT GGACCATTAC CTGCGGGCTG TGCACGGCAG CCCAACACAT
    CCCGCCATCT CTGCAGCCAG GGGCCTCAGC CCCGCGGATG TGGCTCACGA GCACCTTAAG
    GAGCGGGGGC TCTTTGGCCT TCCCGCTGCA GGCACCAACG CCTCAGACTA TTACCACCAG
    ATGACTCTCA TGGCAGGTCA CCCCGCGCCA TATGGGGACC TGCTGATGCA GACTGGGGGT
    GCGGCCAGTG CACCCCATCT CCATGACTAC CTCAATCCAG TGGATGTGTC ACGTTTCTCC
    AGCCCCCGGG TGACACCCCG CCTGAGCCGC AAGCGGGCGC TGTCCATCTC CCCGCTCTCA
    GACGCCAGCC TCGACCTCCA GCGCATGATC CGGACCTCGC CCAACTCGCT GGTGGCCTAC
    ATCAACAACT CCCGCAGCAG CTCGGCTGCC AGCGGCTCCT ACGGACACCT GTCGGCCGGT
    GCCCTCAGCC CAGCCTTCAC CTTCCCCCAC CCCATCAACC CCGTGGCCTA CCAGCAGATC
    CTGAGCCAGC AGAGGGGCCT GGGCTCAGCC TTCGGACACA CGCCACCCCT GATCCAGCCC
    TCGCCCACCT TCCTGGCCCA GCAGCCAGTG GCCTTCACCT CCATCAATGC CACACCCACC
    CAGCTCAGTA GCAGCAGCAA CTGTCTGAGT GACGCCAATC AGAACAAGCA GAGCAGCGAG
    TCAGCCGTGA GCAGCACTGT CAACCCCATC ATCATTCACA AGCGCAGCAA GGTCAAAACT
    GAGGCTGACG GCCTACGGCC AGCCTCCCCA CTGACCCTAA CACAGGAGCA GCTGGCTGAC
    CTCAAGGAGG ACCTGGAGAG AGACGATTGT AAGCAGGAGG CTGAGGTGGT CATCTATGAG
    ACCAACTGCC ACTGGGAGGA CTGCACCAAG GAGTACGACA CGCAGGAGCA GCTGGTGCAC
    CACATCAACA ACGAGCACAT CCACGGGGAG AAGAAGGAGT TTGTGTGCCG CTGGCAGGCC
    TGCACGCGTG AGCAGAAGCC CTTCAAGGCA CAGTACATGC TTGTGGTGCA CATGCGCAGA
    CACACAGGCG AGAAGCCCCA CAAGTGCACG TTCGAGGGCT GCTCAAAGGC CTATTCCCGC
    CTGGAGAACC TGAAGACTCA CCTGCGCTCC CACACCGGGG AAAAGCCGTA TGTGTGTGAG
    CACGAGGGCT GCAACAAAGC CTTCTCCAAT GCCTCAGACC GCGCCAAGCA CCAGAACCGC
    ACCCACTCCA ACGAGAAACC CTACATCTGC AAGATTCCAG GCTGCACCAA GCGGTACACG
    GACCCCAGCT CCCTCCGGAA GCACGTGAAG ACGGTCCATG GCCCAGACGC TCACGTCACC
    AAGAAACAGC GCAACGATGT GCACCTCCGC GCCCCTCTGC TCCGGGAGAA TGGGGACAGC
    GAGGCCGGTG CCGAGCCCGG CCGGGGCTCC GAGGACAACG CCGAGGCCAG CAGCACTAGC
    CAGGCCGTGG AGGACGGCCT GCACGTCAAG GCCATCAAGA CGGAGCGCTC CGGGCTGTGT
    CAGTCCAGCC CCGGAGCCCA GTCTTCCTGC AGCAGCGAGC CCTCTCCCCT GGGCAGTGCC
    CCCAACAATG ACAGTGGCGT GGAGATGCCG GGGACAGGGC CCGGGAGCCT GGCAGACCTG
    ACAGCTCTGG AGGACCCGCC GCCAGGGGCT GACACCTCCG CCCTGGCCGC CCCCTCTGCT
    GGTGGCCTAC AGCTGCGCAA ACACATGACC GCTATGCACC GGTTCGAGCA GCTCAAGAAG
    GAGAAACTCA AGTCACTGAA GGATTCCTGC TCATGGGCCG GGCCAGCTCC GCACACCCGG
    ACCTCCAAGC TGCCTCCCCT CCCGGGAAGT GGCTCCGTGC TGGAAAACTT CAGCGGCGGC
    GGGGGCGGCG GACCCGCGGG GCTGCTGCCC AACCCGCGGC TGTCTGAGCT GTTCCCGGGC
    GAGGTGACAG TGCTGAGCCA CCTGCAGGAA CGCCGGGACA GCTCCACCAG CACAGTCAGC
    TCGGCCTACA CCGTGAGCCG CCGCTCCTCC GGCATCTCCC CCTACTTCTC TAGTCGCCGC
    TCCAGCGAGG CCTCGCCGCT GGGCGCCGGC CGCGCGCACC CTGCCAGCTC TGCTGATTCC
    TACGACCCCA TCTCCACCGA CGCATCACGG CGCTCGAGTG AGGCCAGTCA GTGCGGCGGC
    GGCCCGGGGC TTCTCCAGCT CACACCCGCC CAGCAGTACA GCCTACGGGC CAAGTACGCA
    GCAGCCACAG GTGGACCTCC CCCGACCCCG CTGCCGGGCC TGGAGCGCAC GAGCCTGCGG
    ACCCGGCTGG CGCTGCTGGA TGCTCCCGAG CGCGCGCTGC CCGCTGCCTG CCTGCGCCCG
    ATCGGGCCTC GGCGGGGCAG CGACGGGCCA CACTACGGGC CCTCGGGGCC GTTGCCCGCT
    TTCCCCCACG AGGCACCAGG CGGCGGGGCG CGGCGGGCCA GCGATCCTGG GAGGCGGCCT
    GATGCCCTGG CAGCCCCGCG GGTGCAGCGC TTCCACAGCG CCCTCGACGT GACCCCAGGC
    ACGCTACCGC CACCCTGCGG CCAGGGCCGA GGCCTCCGTC TGCAAAGCCA CACGAGCGCA
    GACGGCAGCC TGGCCCGCAG CGGCTACTCG CCCCGGCCGC CCAGCATCAG CGAGAACGTG
    GTGATGGAAG CCTTGGCGGC GGGGGCGGAC GGCACAGGCC CAGAGGTCAG CCTCGGACTG
    CCAGAGGACG ACCTCGTGCT CCCCGATGAC GTGGTGCAGT ACATCAAGGC ACGTACCAGT
    GGCACCCTGG AGGAGAGTAC CCCACCGGTG TACCCCCCGG AAAGCACCTG CTTCTCTGAG
    AACCCCAAAC TGCCCAGCCC CGGGCTGCAC GGCCAACGTA CCATAGCAGC TGCAGACTCA
    AACGTGGGCC CCTCCGCCCC GGTGCTGGGA GGCTGCCCAC TGGGCTACGG GGCCCCCTCC
    AGCCTGAACA AGAACAGCAT GCCTGTGCAG TGGAACGAAG TGAGCTCAGG CACCGTGGAC
    GCACTGGCCA GCCAGGTGAA GCCTCTGCCC TTCCCACAGG GCAACCTAGC TGTGGTACCA
    CAGAAACCAG CCTTCGCCCA GTACCCAGGA TACAATCCGC AGGGCCTGCA GCCGAGCCCT
    GGGGGCCTAG ACAACTCACA GCCACACTTA CAGCCCTACA GTGGAGCCCC ATCCGCACCC
    AGGGGAAATT ACACACAGCA GCTGCGGCAG CCAGGGGCTG GCAGCCAGTG TCCCAACATG
    ACTGCTGCAG TGAACCCCCA CCCCGGCTAT GGCCAAGCCC ACCCCCAGCT GAGCACCATG
    GGTGGGACCC TGAACCAATT CCCCTCCTCT TGCAGCAACA TGGCAACCAA GCCAGGCCAC
    CTGGGGCTCC CCCAGCAAAT GGAAGTTGCT CCTGACCCCA CCATGATGGG CAACAGCCAC
    AGGGAGCTCG GCATGCCCAA TTCCAGCCTG GCCGGGATGC CACCACCTCA TCCAGCACAG
    AACTACCCAC AGCAGAGTCA TCACCTGGTG ACCACCATGA GCCAGGAGGG CTACCACCAA
    GGCCCCAACC TGGTACCTTC CCACCAACCT AGTTTCATGG AGCCCCAGCA GGGCACAATG
    GGGGCAGCTG GATCTAGCTT TGGCCTAATG CAACCCCGGC CACCCCCAGA GCCTGGCCCT
    GCCGGCCGCC ACCGTGGGGT ACGTGCAGGG CAGCAGCTAG CCTATGCCAG GGCAACTGGC
    CATGCCATGG CTGCTATGTC AGCCAACCAG GAGATGACAG AGTCTGTACC CAAGGGGACA
    ATGGGCAGCA TGTTGTCCCT ACCCCCTCAG CCACCCCCAC AGGACACAGG TGGGACCCAG
    GACCACAGCA TGCTCTACTA CTATGGCCAG ATCCACATGT ACGAGCAGAA CGGAGGCCCT
    GAGAACCAGG CCGGCTGCCA AGCCCTGCGA CCCCAGCCAA CACAGCCCCA GGCCTTGCCC
    TCGCCGGGGG TCAACCAGGT GTCCAGCACT GTGGACTCCC AGCTACTTGA GGCACCCCAG
    ATTGATTTTG ATGCCATCAT GGATGACAGT GATCACTCCA GCTTGCTTTC TGGCGCCCTG
    AGCCCCAGCC TCCTCCACGG CCTCTCCCAG AGCTCCTCCC GCCTCACCAC CCCACGCAGC
    TCCCTGACTC TGCCCTCCAT CCCCGCCGGT ATCAGCAACA TGGCCGTGGG GGACATGAGC
    TCCATGCTCA CCAGCCTGGC CGAGGAGAGC AAGTTCTTGA ACATGATGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015313566.1
    CDS57..4730
    Translation

    Target ORF information:

    RefSeq Version XM_015458080.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458080.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGACAT CCGCCTCAGC CACGGCCACG GACAAGAAAG AAGTCAAAGG TGGGATCCTG 
    GAGGGCCTTG CTTTCCCTGA CCCCGGCAGG AAAGCCTCGG CCCTCGCGGT GGCCACGGCG
    GCGGCTGCGG CCGCAGTAGC TGCCCAAGGA GTGCCGCCGC ATCTCCTGCC ACCGTTCCAC
    GCCCCCTTCC CGATTGACAT GCGACACCAG GAGGGACGGT ACCATTACGA GCCTCACTCT
    GTCCCCGGCG TGCACGGGCC TCCCGCCCTG AGCGGCAGCC CTGTCATCTC TGATATCTCA
    TTGATTCGGC TTTCCCCACA CCCTGCCGGC CCTGGGGAGT CCCCCTTCAA CGCCCCCCAC
    CCGTACGTGA GCCCCCACAT GGACCATTAC CTGCGGGCTG TGCACGGCAG CCCAACACAT
    CCCGCCATCT CTGCAGCCAG GGGCCTCAGC CCCGCGGATG TGGCTCACGA GCACCTTAAG
    GAGCGGGGGC TCTTTGGCCT TCCCGCTGCA GGCACCAACG CCTCAGACTA TTACCACCAG
    ATGACTCTCA TGGCAGGTCA CCCCGCGCCA TATGGGGACC TGCTGATGCA GACTGGGGGT
    GCGGCCAGTG CACCCCATCT CCATGACTAC CTCAATCCAG TGGATGTGTC ACGTTTCTCC
    AGCCCCCGGG TGACACCCCG CCTGAGCCGC AAGCGGGCGC TGTCCATCTC CCCGCTCTCA
    GACGCCAGCC TCGACCTCCA GCGCATGATC CGGACCTCGC CCAACTCGCT GGTGGCCTAC
    ATCAACAACT CCCGCAGCAG CTCGGCTGCC AGCGGCTCCT ACGGACACCT GTCGGCCGGT
    GCCCTCAGCC CAGCCTTCAC CTTCCCCCAC CCCATCAACC CCGTGGCCTA CCAGCAGATC
    CTGAGCCAGC AGAGGGGCCT GGGCTCAGCC TTCGGACACA CGCCACCCCT GATCCAGCCC
    TCGCCCACCT TCCTGGCCCA GCAGCCAGTG GCCTTCACCT CCATCAATGC CACACCCACC
    CAGCTCAGTA GCAGCAGCAA CTGTCTGAGT GACGCCAATC AGAACAAGCA GAGCAGCGAG
    TCAGCCGTGA GCAGCACTGT CAACCCCATC ATCATTCACA AGCGCAGCAA GGTCAAAACT
    GAGGCTGACG GCCTACGGCC AGCCTCCCCA CTGACCCTAA CACAGGAGCA GCTGGCTGAC
    CTCAAGGAGG ACCTGGAGAG AGACGATTGT AAGCAGGAGG CTGAGGTGGT CATCTATGAG
    ACCAACTGCC ACTGGGAGGA CTGCACCAAG GAGTACGACA CGCAGGAGCA GCTGGTGCAC
    CACATCAACA ACGAGCACAT CCACGGGGAG AAGAAGGAGT TTGTGTGCCG CTGGCAGGCC
    TGCACGCGTG AGCAGAAGCC CTTCAAGGCA CAGTACATGC TTGTGGTGCA CATGCGCAGA
    CACACAGGCG AGAAGCCCCA CAAGTGCACG TTCGAGGGCT GCTCAAAGGC CTATTCCCGC
    CTGGAGAACC TGAAGACTCA CCTGCGCTCC CACACCGGGG AAAAGCCGTA TGTGTGTGAG
    CACGAGGGCT GCAACAAAGC CTTCTCCAAT GCCTCAGACC GCGCCAAGCA CCAGAACCGC
    ACCCACTCCA ACGAGAAACC CTACATCTGC AAGATTCCAG GCTGCACCAA GCGGTACACG
    GACCCCAGCT CCCTCCGGAA GCACGTGAAG ACGGTCCATG GCCCAGACGC TCACGTCACC
    AAGAAACAGC GCAACGATGT GCACCTCCGC GCCCCTCTGC TCCGGGAGAA TGGGGACAGC
    GAGGCCGGTG CCGAGCCCGG CCGGGGCTCC GAGGACAACG CCGAGGCCAG CAGCACTAGC
    CAGGCCGTGG AGGACGGCCT GCACGTCAAG GCCATCAAGA CGGAGCGCTC CGGGCTGTGT
    CAGTCCAGCC CCGGAGCCCA GTCTTCCTGC AGCAGCGAGC CCTCTCCCCT GGGCAGTGCC
    CCCAACAATG ACAGTGGCGT GGAGATGCCG GGGACAGGGC CCGGGAGCCT GGCAGACCTG
    ACAGCTCTGG AGGACCCGCC GCCAGGGGCT GACACCTCCG CCCTGGCCGC CCCCTCTGCT
    GGTGGCCTAC AGCTGCGCAA ACACATGACC GCTATGCACC GGTTCGAGCA GCTCAAGAAG
    GAGAAACTCA AGTCACTGAA GGATTCCTGC TCATGGGCCG GGCCAGCTCC GCACACCCGG
    ACCTCCAAGC TGCCTCCCCT CCCGGGAAGT GGCTCCGTGC TGGAAAACTT CAGCGGCGGC
    GGGGGCGGCG GACCCGCGGG GCTGCTGCCC AACCCGCGGC TGTCTGAGCT GTTCCCGGGC
    GAGGTGACAG TGCTGAGCCA CCTGCAGGAA CGCCGGGACA GCTCCACCAG CACAGTCAGC
    TCGGCCTACA CCGTGAGCCG CCGCTCCTCC GGCATCTCCC CCTACTTCTC TAGTCGCCGC
    TCCAGCGAGG CCTCGCCGCT GGGCGCCGGC CGCGCGCACC CTGCCAGCTC TGCTGATTCC
    TACGACCCCA TCTCCACCGA CGCATCACGG CGCTCGAGTG AGGCCAGTCA GTGCGGCGGC
    GGCCCGGGGC TTCTCCAGCT CACACCCGCC CAGCAGTACA GCCTACGGGC CAAGTACGCA
    GCAGCCACAG GTGGACCTCC CCCGACCCCG CTGCCGGGCC TGGAGCGCAC GAGCCTGCGG
    ACCCGGCTGG CGCTGCTGGA TGCTCCCGAG CGCGCGCTGC CCGCTGCCTG CCTGCGCCCG
    ATCGGGCCTC GGCGGGGCAG CGACGGGCCA CACTACGGGC CCTCGGGGCC GTTGCCCGCT
    TTCCCCCACG AGGCACCAGG CGGCGGGGCG CGGCGGGCCA GCGATCCTGG GAGGCGGCCT
    GATGCCCTGG CAGCCCCGCG GGTGCAGCGC TTCCACAGCG CCCTCGACGT GACCCCAGGC
    ACGCTACCGC CACCCTGCGG CCAGGGCCGA GGCCTCCGTC TGCAAAGCCA CACGAGCGCA
    GACGGCAGCC TGGCCCGCAG CGGCTACTCG CCCCGGCCGC CCAGCATCAG CGAGAACGTG
    GTGATGGAAG CCTTGGCGGC GGGGGCGGAC GGCACAGGCC CAGAGGTCAG CCTCGGACTG
    CCAGAGGACG ACCTCGTGCT CCCCGATGAC GTGGTGCAGT ACATCAAGGC ACGTACCAGT
    GGCACCCTGG AGGAGAGTAC CCCACCGGTG TACCCCCCGG AAAGCACCTG CTTCTCTGAG
    AACCCCAAAC TGCCCAGCCC CGGGCTGCAC GGCCAACGTA CCATAGCAGC TGCAGACTCA
    AACGTGGGCC CCTCCGCCCC GGTGCTGGGA GGCTGCCCAC TGGGCTACGG GGCCCCCTCC
    AGCCTGAACA AGAACAGCAT GCCTGTGCAG TGGAACGAAG TGAGCTCAGG CACCGTGGAC
    GCACTGGCCA GCCAGGTGAA GCCTCTGCCC TTCCCACAGG GCAACCTAGC TGTGGTACCA
    CAGAAACCAG CCTTCGCCCA GTACCCAGGA TACAATCCGC AGGGCCTGCA GCCGAGCCCT
    GGGGGCCTAG ACAACTCACA GCCACACTTA CAGCCCTACA GTGGAGCCCC ATCCGCACCC
    AGGGGAAATT ACACACAGCA GCTGCGGCAG CCAGGGGCTG GCAGCCAGTG TCCCAACATG
    ACTGCTGCAG TGAACCCCCA CCCCGGCTAT GGCCAAGCCC ACCCCCAGCT GAGCACCATG
    GGTGGGACCC TGAACCAATT CCCCTCCTCT TGCAGCAACA TGGCAACCAA GCCAGGCCAC
    CTGGGGCTCC CCCAGCAAAT GGAAGTTGCT CCTGACCCCA CCATGATGGG CAACAGCCAC
    AGGGAGCTCG GCATGCCCAA TTCCAGCCTG GCCGGGATGC CACCACCTCA TCCAGCACAG
    AACTACCCAC AGCAGAGTCA TCACCTGGTG ACCACCATGA GCCAGGAGGG CTACCACCAA
    GGCCCCAACC TGGTACCTTC CCACCAACCT AGTTTCATGG AGCCCCAGCA GGGCACAATG
    GGGGCAGCTG GATCTAGCTT TGGCCTAATG CAACCCCGGC CACCCCCAGA GCCTGGCCCT
    GCCGGCCGCC ACCGTGGGGT ACGTGCAGGG CAGCAGCTAG CCTATGCCAG GGCAACTGGC
    CATGCCATGG CTGCTATGTC AGCCAACCAG GAGATGACAG AGTCTGTACC CAAGGGGACA
    ATGGGCAGCA TGTTGTCCCT ACCCCCTCAG CCACCCCCAC AGGACACAGG TGGGACCCAG
    GACCACAGCA TGCTCTACTA CTATGGCCAG ATCCACATGT ACGAGCAGAA CGGAGGCCCT
    GAGAACCAGG CCGGCTGCCA AGCCCTGCGA CCCCAGCCAA CACAGCCCCA GGCCTTGCCC
    TCGCCGGGGG TCAACCAGGT GTCCAGCACT GTGGACTCCC AGCTACTTGA GGCACCCCAG
    ATTGATTTTG ATGCCATCAT GGATGACAGT GATCACTCCA GCTTGCTTTC TGGCGCCCTG
    AGCCCCAGCC TCCTCCACGG CCTCTCCCAG AGCTCCTCCC GCCTCACCAC CCCACGCAGC
    TCCCTGACTC TGCCCTCCAT CCCCGCCGGT ATCAGCAACA TGGCCGTGGG GGACATGAGC
    TCCATGCTCA CCAGCCTGGC CGAGGAGAGC AAGTTCTTGA ACATGATGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87936
    Clone ID Related Accession (Same CDS sequence) XM_015458080.1 , XM_015458080.2
    Accession Version XM_015458080.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein GLI2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGACAT CCGCCTCAGC CACGGCCACG GACAAGAAAG AAGTCAAAGG TGGGATCCTG 
    GAGGGCCTTG CTTTCCCTGA CCCCGGCAGG AAAGCCTCGG CCCTCGCGGT GGCCACGGCG
    GCGGCTGCGG CCGCAGTAGC TGCCCAAGGA GTGCCGCCGC ATCTCCTGCC ACCGTTCCAC
    GCCCCCTTCC CGATTGACAT GCGACACCAG GAGGGACGGT ACCATTACGA GCCTCACTCT
    GTCCCCGGCG TGCACGGGCC TCCCGCCCTG AGCGGCAGCC CTGTCATCTC TGATATCTCA
    TTGATTCGGC TTTCCCCACA CCCTGCCGGC CCTGGGGAGT CCCCCTTCAA CGCCCCCCAC
    CCGTACGTGA GCCCCCACAT GGACCATTAC CTGCGGGCTG TGCACGGCAG CCCAACACAT
    CCCGCCATCT CTGCAGCCAG GGGCCTCAGC CCCGCGGATG TGGCTCACGA GCACCTTAAG
    GAGCGGGGGC TCTTTGGCCT TCCCGCTGCA GGCACCAACG CCTCAGACTA TTACCACCAG
    ATGACTCTCA TGGCAGGTCA CCCCGCGCCA TATGGGGACC TGCTGATGCA GACTGGGGGT
    GCGGCCAGTG CACCCCATCT CCATGACTAC CTCAATCCAG TGGATGTGTC ACGTTTCTCC
    AGCCCCCGGG TGACACCCCG CCTGAGCCGC AAGCGGGCGC TGTCCATCTC CCCGCTCTCA
    GACGCCAGCC TCGACCTCCA GCGCATGATC CGGACCTCGC CCAACTCGCT GGTGGCCTAC
    ATCAACAACT CCCGCAGCAG CTCGGCTGCC AGCGGCTCCT ACGGACACCT GTCGGCCGGT
    GCCCTCAGCC CAGCCTTCAC CTTCCCCCAC CCCATCAACC CCGTGGCCTA CCAGCAGATC
    CTGAGCCAGC AGAGGGGCCT GGGCTCAGCC TTCGGACACA CGCCACCCCT GATCCAGCCC
    TCGCCCACCT TCCTGGCCCA GCAGCCAGTG GCCTTCACCT CCATCAATGC CACACCCACC
    CAGCTCAGTA GCAGCAGCAA CTGTCTGAGT GACGCCAATC AGAACAAGCA GAGCAGCGAG
    TCAGCCGTGA GCAGCACTGT CAACCCCATC ATCATTCACA AGCGCAGCAA GGTCAAAACT
    GAGGCTGACG GCCTACGGCC AGCCTCCCCA CTGACCCTAA CACAGGAGCA GCTGGCTGAC
    CTCAAGGAGG ACCTGGAGAG AGACGATTGT AAGCAGGAGG CTGAGGTGGT CATCTATGAG
    ACCAACTGCC ACTGGGAGGA CTGCACCAAG GAGTACGACA CGCAGGAGCA GCTGGTGCAC
    CACATCAACA ACGAGCACAT CCACGGGGAG AAGAAGGAGT TTGTGTGCCG CTGGCAGGCC
    TGCACGCGTG AGCAGAAGCC CTTCAAGGCA CAGTACATGC TTGTGGTGCA CATGCGCAGA
    CACACAGGCG AGAAGCCCCA CAAGTGCACG TTCGAGGGCT GCTCAAAGGC CTATTCCCGC
    CTGGAGAACC TGAAGACTCA CCTGCGCTCC CACACCGGGG AAAAGCCGTA TGTGTGTGAG
    CACGAGGGCT GCAACAAAGC CTTCTCCAAT GCCTCAGACC GCGCCAAGCA CCAGAACCGC
    ACCCACTCCA ACGAGAAACC CTACATCTGC AAGATTCCAG GCTGCACCAA GCGGTACACG
    GACCCCAGCT CCCTCCGGAA GCACGTGAAG ACGGTCCATG GCCCAGACGC TCACGTCACC
    AAGAAACAGC GCAACGATGT GCACCTCCGC GCCCCTCTGC TCCGGGAGAA TGGGGACAGC
    GAGGCCGGTG CCGAGCCCGG CCGGGGCTCC GAGGACAACG CCGAGGCCAG CAGCACTAGC
    CAGGCCGTGG AGGACGGCCT GCACGTCAAG GCCATCAAGA CGGAGCGCTC CGGGCTGTGT
    CAGTCCAGCC CCGGAGCCCA GTCTTCCTGC AGCAGCGAGC CCTCTCCCCT GGGCAGTGCC
    CCCAACAATG ACAGTGGCGT GGAGATGCCG GGGACAGGGC CCGGGAGCCT GGCAGACCTG
    ACAGCTCTGG AGGACCCGCC GCCAGGGGCT GACACCTCCG CCCTGGCCGC CCCCTCTGCT
    GGTGGCCTAC AGCTGCGCAA ACACATGACC GCTATGCACC GGTTCGAGCA GCTCAAGAAG
    GAGAAACTCA AGTCACTGAA GGATTCCTGC TCATGGGCCG GGCCAGCTCC GCACACCCGG
    ACCTCCAAGC TGCCTCCCCT CCCGGGAAGT GGCTCCGTGC TGGAAAACTT CAGCGGCGGC
    GGGGGCGGCG GACCCGCGGG GCTGCTGCCC AACCCGCGGC TGTCTGAGCT GTTCCCGGGC
    GAGGTGACAG TGCTGAGCCA CCTGCAGGAA CGCCGGGACA GCTCCACCAG CACAGTCAGC
    TCGGCCTACA CCGTGAGCCG CCGCTCCTCC GGCATCTCCC CCTACTTCTC TAGTCGCCGC
    TCCAGCGAGG CCTCGCCGCT GGGCGCCGGC CGCGCGCACC CTGCCAGCTC TGCTGATTCC
    TACGACCCCA TCTCCACCGA CGCATCACGG CGCTCGAGTG AGGCCAGTCA GTGCGGCGGC
    GGCCCGGGGC TTCTCCAGCT CACACCCGCC CAGCAGTACA GCCTACGGGC CAAGTACGCA
    GCAGCCACAG GTGGACCTCC CCCGACCCCG CTGCCGGGCC TGGAGCGCAC GAGCCTGCGG
    ACCCGGCTGG CGCTGCTGGA TGCTCCCGAG CGCGCGCTGC CCGCTGCCTG CCTGCGCCCG
    ATCGGGCCTC GGCGGGGCAG CGACGGGCCA CACTACGGGC CCTCGGGGCC GTTGCCCGCT
    TTCCCCCACG AGGCACCAGG CGGCGGGGCG CGGCGGGCCA GCGATCCTGG GAGGCGGCCT
    GATGCCCTGG CAGCCCCGCG GGTGCAGCGC TTCCACAGCG CCCTCGACGT GACCCCAGGC
    ACGCTACCGC CACCCTGCGG CCAGGGCCGA GGCCTCCGTC TGCAAAGCCA CACGAGCGCA
    GACGGCAGCC TGGCCCGCAG CGGCTACTCG CCCCGGCCGC CCAGCATCAG CGAGAACGTG
    GTGATGGAAG CCTTGGCGGC GGGGGCGGAC GGCACAGGCC CAGAGGTCAG CCTCGGACTG
    CCAGAGGACG ACCTCGTGCT CCCCGATGAC GTGGTGCAGT ACATCAAGGC ACGTACCAGT
    GGCACCCTGG AGGAGAGTAC CCCACCGGTG TACCCCCCGG AAAGCACCTG CTTCTCTGAG
    AACCCCAAAC TGCCCAGCCC CGGGCTGCAC GGCCAACGTA CCATAGCAGC TGCAGACTCA
    AACGTGGGCC CCTCCGCCCC GGTGCTGGGA GGCTGCCCAC TGGGCTACGG GGCCCCCTCC
    AGCCTGAACA AGAACAGCAT GCCTGTGCAG TGGAACGAAG TGAGCTCAGG CACCGTGGAC
    GCACTGGCCA GCCAGGTGAA GCCTCTGCCC TTCCCACAGG GCAACCTAGC TGTGGTACCA
    CAGAAACCAG CCTTCGCCCA GTACCCAGGA TACAATCCGC AGGGCCTGCA GCCGAGCCCT
    GGGGGCCTAG ACAACTCACA GCCACACTTA CAGCCCTACA GTGGAGCCCC ATCCGCACCC
    AGGGGAAATT ACACACAGCA GCTGCGGCAG CCAGGGGCTG GCAGCCAGTG TCCCAACATG
    ACTGCTGCAG TGAACCCCCA CCCCGGCTAT GGCCAAGCCC ACCCCCAGCT GAGCACCATG
    GGTGGGACCC TGAACCAATT CCCCTCCTCT TGCAGCAACA TGGCAACCAA GCCAGGCCAC
    CTGGGGCTCC CCCAGCAAAT GGAAGTTGCT CCTGACCCCA CCATGATGGG CAACAGCCAC
    AGGGAGCTCG GCATGCCCAA TTCCAGCCTG GCCGGGATGC CACCACCTCA TCCAGCACAG
    AACTACCCAC AGCAGAGTCA TCACCTGGTG ACCACCATGA GCCAGGAGGG CTACCACCAA
    GGCCCCAACC TGGTACCTTC CCACCAACCT AGTTTCATGG AGCCCCAGCA GGGCACAATG
    GGGGCAGCTG GATCTAGCTT TGGCCTAATG CAACCCCGGC CACCCCCAGA GCCTGGCCCT
    GCCGGCCGCC ACCGTGGGGT ACGTGCAGGG CAGCAGCTAG CCTATGCCAG GGCAACTGGC
    CATGCCATGG CTGCTATGTC AGCCAACCAG GAGATGACAG AGTCTGTACC CAAGGGGACA
    ATGGGCAGCA TGTTGTCCCT ACCCCCTCAG CCACCCCCAC AGGACACAGG TGGGACCCAG
    GACCACAGCA TGCTCTACTA CTATGGCCAG ATCCACATGT ACGAGCAGAA CGGAGGCCCT
    GAGAACCAGG CCGGCTGCCA AGCCCTGCGA CCCCAGCCAA CACAGCCCCA GGCCTTGCCC
    TCGCCGGGGG TCAACCAGGT GTCCAGCACT GTGGACTCCC AGCTACTTGA GGCACCCCAG
    ATTGATTTTG ATGCCATCAT GGATGACAGT GATCACTCCA GCTTGCTTTC TGGCGCCCTG
    AGCCCCAGCC TCCTCCACGG CCTCTCCCAG AGCTCCTCCC GCCTCACCAC CCCACGCAGC
    TCCCTGACTC TGCCCTCCAT CCCCGCCGGT ATCAGCAACA TGGCCGTGGG GGACATGAGC
    TCCATGCTCA CCAGCCTGGC CGAGGAGAGC AAGTTCTTGA ACATGATGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015313566.1
    CDS55..4728
    Translation

    Target ORF information:

    RefSeq Version XM_015458080.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458080.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGACAT CCGCCTCAGC CACGGCCACG GACAAGAAAG AAGTCAAAGG TGGGATCCTG 
    GAGGGCCTTG CTTTCCCTGA CCCCGGCAGG AAAGCCTCGG CCCTCGCGGT GGCCACGGCG
    GCGGCTGCGG CCGCAGTAGC TGCCCAAGGA GTGCCGCCGC ATCTCCTGCC ACCGTTCCAC
    GCCCCCTTCC CGATTGACAT GCGACACCAG GAGGGACGGT ACCATTACGA GCCTCACTCT
    GTCCCCGGCG TGCACGGGCC TCCCGCCCTG AGCGGCAGCC CTGTCATCTC TGATATCTCA
    TTGATTCGGC TTTCCCCACA CCCTGCCGGC CCTGGGGAGT CCCCCTTCAA CGCCCCCCAC
    CCGTACGTGA GCCCCCACAT GGACCATTAC CTGCGGGCTG TGCACGGCAG CCCAACACAT
    CCCGCCATCT CTGCAGCCAG GGGCCTCAGC CCCGCGGATG TGGCTCACGA GCACCTTAAG
    GAGCGGGGGC TCTTTGGCCT TCCCGCTGCA GGCACCAACG CCTCAGACTA TTACCACCAG
    ATGACTCTCA TGGCAGGTCA CCCCGCGCCA TATGGGGACC TGCTGATGCA GACTGGGGGT
    GCGGCCAGTG CACCCCATCT CCATGACTAC CTCAATCCAG TGGATGTGTC ACGTTTCTCC
    AGCCCCCGGG TGACACCCCG CCTGAGCCGC AAGCGGGCGC TGTCCATCTC CCCGCTCTCA
    GACGCCAGCC TCGACCTCCA GCGCATGATC CGGACCTCGC CCAACTCGCT GGTGGCCTAC
    ATCAACAACT CCCGCAGCAG CTCGGCTGCC AGCGGCTCCT ACGGACACCT GTCGGCCGGT
    GCCCTCAGCC CAGCCTTCAC CTTCCCCCAC CCCATCAACC CCGTGGCCTA CCAGCAGATC
    CTGAGCCAGC AGAGGGGCCT GGGCTCAGCC TTCGGACACA CGCCACCCCT GATCCAGCCC
    TCGCCCACCT TCCTGGCCCA GCAGCCAGTG GCCTTCACCT CCATCAATGC CACACCCACC
    CAGCTCAGTA GCAGCAGCAA CTGTCTGAGT GACGCCAATC AGAACAAGCA GAGCAGCGAG
    TCAGCCGTGA GCAGCACTGT CAACCCCATC ATCATTCACA AGCGCAGCAA GGTCAAAACT
    GAGGCTGACG GCCTACGGCC AGCCTCCCCA CTGACCCTAA CACAGGAGCA GCTGGCTGAC
    CTCAAGGAGG ACCTGGAGAG AGACGATTGT AAGCAGGAGG CTGAGGTGGT CATCTATGAG
    ACCAACTGCC ACTGGGAGGA CTGCACCAAG GAGTACGACA CGCAGGAGCA GCTGGTGCAC
    CACATCAACA ACGAGCACAT CCACGGGGAG AAGAAGGAGT TTGTGTGCCG CTGGCAGGCC
    TGCACGCGTG AGCAGAAGCC CTTCAAGGCA CAGTACATGC TTGTGGTGCA CATGCGCAGA
    CACACAGGCG AGAAGCCCCA CAAGTGCACG TTCGAGGGCT GCTCAAAGGC CTATTCCCGC
    CTGGAGAACC TGAAGACTCA CCTGCGCTCC CACACCGGGG AAAAGCCGTA TGTGTGTGAG
    CACGAGGGCT GCAACAAAGC CTTCTCCAAT GCCTCAGACC GCGCCAAGCA CCAGAACCGC
    ACCCACTCCA ACGAGAAACC CTACATCTGC AAGATTCCAG GCTGCACCAA GCGGTACACG
    GACCCCAGCT CCCTCCGGAA GCACGTGAAG ACGGTCCATG GCCCAGACGC TCACGTCACC
    AAGAAACAGC GCAACGATGT GCACCTCCGC GCCCCTCTGC TCCGGGAGAA TGGGGACAGC
    GAGGCCGGTG CCGAGCCCGG CCGGGGCTCC GAGGACAACG CCGAGGCCAG CAGCACTAGC
    CAGGCCGTGG AGGACGGCCT GCACGTCAAG GCCATCAAGA CGGAGCGCTC CGGGCTGTGT
    CAGTCCAGCC CCGGAGCCCA GTCTTCCTGC AGCAGCGAGC CCTCTCCCCT GGGCAGTGCC
    CCCAACAATG ACAGTGGCGT GGAGATGCCG GGGACAGGGC CCGGGAGCCT GGCAGACCTG
    ACAGCTCTGG AGGACCCGCC GCCAGGGGCT GACACCTCCG CCCTGGCCGC CCCCTCTGCT
    GGTGGCCTAC AGCTGCGCAA ACACATGACC GCTATGCACC GGTTCGAGCA GCTCAAGAAG
    GAGAAACTCA AGTCACTGAA GGATTCCTGC TCATGGGCCG GGCCAGCTCC GCACACCCGG
    ACCTCCAAGC TGCCTCCCCT CCCGGGAAGT GGCTCCGTGC TGGAAAACTT CAGCGGCGGC
    GGGGGCGGCG GACCCGCGGG GCTGCTGCCC AACCCGCGGC TGTCTGAGCT GTTCCCGGGC
    GAGGTGACAG TGCTGAGCCA CCTGCAGGAA CGCCGGGACA GCTCCACCAG CACAGTCAGC
    TCGGCCTACA CCGTGAGCCG CCGCTCCTCC GGCATCTCCC CCTACTTCTC TAGTCGCCGC
    TCCAGCGAGG CCTCGCCGCT GGGCGCCGGC CGCGCGCACC CTGCCAGCTC TGCTGATTCC
    TACGACCCCA TCTCCACCGA CGCATCACGG CGCTCGAGTG AGGCCAGTCA GTGCGGCGGC
    GGCCCGGGGC TTCTCCAGCT CACACCCGCC CAGCAGTACA GCCTACGGGC CAAGTACGCA
    GCAGCCACAG GTGGACCTCC CCCGACCCCG CTGCCGGGCC TGGAGCGCAC GAGCCTGCGG
    ACCCGGCTGG CGCTGCTGGA TGCTCCCGAG CGCGCGCTGC CCGCTGCCTG CCTGCGCCCG
    ATCGGGCCTC GGCGGGGCAG CGACGGGCCA CACTACGGGC CCTCGGGGCC GTTGCCCGCT
    TTCCCCCACG AGGCACCAGG CGGCGGGGCG CGGCGGGCCA GCGATCCTGG GAGGCGGCCT
    GATGCCCTGG CAGCCCCGCG GGTGCAGCGC TTCCACAGCG CCCTCGACGT GACCCCAGGC
    ACGCTACCGC CACCCTGCGG CCAGGGCCGA GGCCTCCGTC TGCAAAGCCA CACGAGCGCA
    GACGGCAGCC TGGCCCGCAG CGGCTACTCG CCCCGGCCGC CCAGCATCAG CGAGAACGTG
    GTGATGGAAG CCTTGGCGGC GGGGGCGGAC GGCACAGGCC CAGAGGTCAG CCTCGGACTG
    CCAGAGGACG ACCTCGTGCT CCCCGATGAC GTGGTGCAGT ACATCAAGGC ACGTACCAGT
    GGCACCCTGG AGGAGAGTAC CCCACCGGTG TACCCCCCGG AAAGCACCTG CTTCTCTGAG
    AACCCCAAAC TGCCCAGCCC CGGGCTGCAC GGCCAACGTA CCATAGCAGC TGCAGACTCA
    AACGTGGGCC CCTCCGCCCC GGTGCTGGGA GGCTGCCCAC TGGGCTACGG GGCCCCCTCC
    AGCCTGAACA AGAACAGCAT GCCTGTGCAG TGGAACGAAG TGAGCTCAGG CACCGTGGAC
    GCACTGGCCA GCCAGGTGAA GCCTCTGCCC TTCCCACAGG GCAACCTAGC TGTGGTACCA
    CAGAAACCAG CCTTCGCCCA GTACCCAGGA TACAATCCGC AGGGCCTGCA GCCGAGCCCT
    GGGGGCCTAG ACAACTCACA GCCACACTTA CAGCCCTACA GTGGAGCCCC ATCCGCACCC
    AGGGGAAATT ACACACAGCA GCTGCGGCAG CCAGGGGCTG GCAGCCAGTG TCCCAACATG
    ACTGCTGCAG TGAACCCCCA CCCCGGCTAT GGCCAAGCCC ACCCCCAGCT GAGCACCATG
    GGTGGGACCC TGAACCAATT CCCCTCCTCT TGCAGCAACA TGGCAACCAA GCCAGGCCAC
    CTGGGGCTCC CCCAGCAAAT GGAAGTTGCT CCTGACCCCA CCATGATGGG CAACAGCCAC
    AGGGAGCTCG GCATGCCCAA TTCCAGCCTG GCCGGGATGC CACCACCTCA TCCAGCACAG
    AACTACCCAC AGCAGAGTCA TCACCTGGTG ACCACCATGA GCCAGGAGGG CTACCACCAA
    GGCCCCAACC TGGTACCTTC CCACCAACCT AGTTTCATGG AGCCCCAGCA GGGCACAATG
    GGGGCAGCTG GATCTAGCTT TGGCCTAATG CAACCCCGGC CACCCCCAGA GCCTGGCCCT
    GCCGGCCGCC ACCGTGGGGT ACGTGCAGGG CAGCAGCTAG CCTATGCCAG GGCAACTGGC
    CATGCCATGG CTGCTATGTC AGCCAACCAG GAGATGACAG AGTCTGTACC CAAGGGGACA
    ATGGGCAGCA TGTTGTCCCT ACCCCCTCAG CCACCCCCAC AGGACACAGG TGGGACCCAG
    GACCACAGCA TGCTCTACTA CTATGGCCAG ATCCACATGT ACGAGCAGAA CGGAGGCCCT
    GAGAACCAGG CCGGCTGCCA AGCCCTGCGA CCCCAGCCAA CACAGCCCCA GGCCTTGCCC
    TCGCCGGGGG TCAACCAGGT GTCCAGCACT GTGGACTCCC AGCTACTTGA GGCACCCCAG
    ATTGATTTTG ATGCCATCAT GGATGACAGT GATCACTCCA GCTTGCTTTC TGGCGCCCTG
    AGCCCCAGCC TCCTCCACGG CCTCTCCCAG AGCTCCTCCC GCCTCACCAC CCCACGCAGC
    TCCCTGACTC TGCCCTCCAT CCCCGCCGGT ATCAGCAACA TGGCCGTGGG GGACATGAGC
    TCCATGCTCA CCAGCCTGGC CGAGGAGAGC AAGTTCTTGA ACATGATGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87937
    Clone ID Related Accession (Same CDS sequence) XM_015458081.1
    Accession Version XM_015458081.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4134bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product zinc finger protein GLI2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103843.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGACTCTCA TGGCAGGTCA CCCCGCGCCA TATGGGGACC TGCTGATGCA GACTGGGGGT 
    GCGGCCAGTG CACCCCATCT CCATGACTAC CTCAATCCAG TGGATGTGTC ACGTTTCTCC
    AGCCCCCGGG TGACACCCCG CCTGAGCCGC AAGCGGGCGC TGTCCATCTC CCCGCTCTCA
    GACGCCAGCC TCGACCTCCA GCGCATGATC CGGACCTCGC CCAACTCGCT GGTGGCCTAC
    ATCAACAACT CCCGCAGCAG CTCGGCTGCC AGCGGCTCCT ACGGACACCT GTCGGCCGGT
    GCCCTCAGCC CAGCCTTCAC CTTCCCCCAC CCCATCAACC CCGTGGCCTA CCAGCAGATC
    CTGAGCCAGC AGAGGGGCCT GGGCTCAGCC TTCGGACACA CGCCACCCCT GATCCAGCCC
    TCGCCCACCT TCCTGGCCCA GCAGCCAGTG GCCTTCACCT CCATCAATGC CACACCCACC
    CAGCTCAGTA GCAGCAGCAA CTGTCTGAGT GACGCCAATC AGAACAAGCA GAGCAGCGAG
    TCAGCCGTGA GCAGCACTGT CAACCCCATC ATCATTCACA AGCGCAGCAA GGTCAAAACT
    GAGGCTGACG GCCTACGGCC AGCCTCCCCA CTGACCCTAA CACAGGAGCA GCTGGCTGAC
    CTCAAGGAGG ACCTGGAGAG AGACGATTGT AAGCAGGAGG CTGAGGTGGT CATCTATGAG
    ACCAACTGCC ACTGGGAGGA CTGCACCAAG GAGTACGACA CGCAGGAGCA GCTGGTGCAC
    CACATCAACA ACGAGCACAT CCACGGGGAG AAGAAGGAGT TTGTGTGCCG CTGGCAGGCC
    TGCACGCGTG AGCAGAAGCC CTTCAAGGCA CAGTACATGC TTGTGGTGCA CATGCGCAGA
    CACACAGGCG AGAAGCCCCA CAAGTGCACG TTCGAGGGCT GCTCAAAGGC CTATTCCCGC
    CTGGAGAACC TGAAGACTCA CCTGCGCTCC CACACCGGGG AAAAGCCGTA TGTGTGTGAG
    CACGAGGGCT GCAACAAAGC CTTCTCCAAT GCCTCAGACC GCGCCAAGCA CCAGAACCGC
    ACCCACTCCA ACGAGAAACC CTACATCTGC AAGATTCCAG GCTGCACCAA GCGGTACACG
    GACCCCAGCT CCCTCCGGAA GCACGTGAAG ACGGTCCATG GCCCAGACGC TCACGTCACC
    AAGAAACAGC GCAACGATGT GCACCTCCGC GCCCCTCTGC TCCGGGAGAA TGGGGACAGC
    GAGGCCGGTG CCGAGCCCGG CCGGGGCTCC GAGGACAACG CCGAGGCCAG CAGCACTAGC
    CAGGCCGTGG AGGACGGCCT GCACGTCAAG GCCATCAAGA CGGAGCGCTC CGGGCTGTGT
    CAGTCCAGCC CCGGAGCCCA GTCTTCCTGC AGCAGCGAGC CCTCTCCCCT GGGCAGTGCC
    CCCAACAATG ACAGTGGCGT GGAGATGCCG GGGACAGGGC CCGGGAGCCT GGCAGACCTG
    ACAGCTCTGG AGGACCCGCC GCCAGGGGCT GACACCTCCG CCCTGGCCGC CCCCTCTGCT
    GGTGGCCTAC AGCTGCGCAA ACACATGACC GCTATGCACC GGTTCGAGCA GCTCAAGAAG
    GAGAAACTCA AGTCACTGAA GGATTCCTGC TCATGGGCCG GGCCAGCTCC GCACACCCGG
    ACCTCCAAGC TGCCTCCCCT CCCGGGAAGT GGCTCCGTGC TGGAAAACTT CAGCGGCGGC
    GGGGGCGGCG GACCCGCGGG GCTGCTGCCC AACCCGCGGC TGTCTGAGCT GTTCCCGGGC
    GAGGTGACAG TGCTGAGCCA CCTGCAGGAA CGCCGGGACA GCTCCACCAG CACAGTCAGC
    TCGGCCTACA CCGTGAGCCG CCGCTCCTCC GGCATCTCCC CCTACTTCTC TAGTCGCCGC
    TCCAGCGAGG CCTCGCCGCT GGGCGCCGGC CGCGCGCACC CTGCCAGCTC TGCTGATTCC
    TACGACCCCA TCTCCACCGA CGCATCACGG CGCTCGAGTG AGGCCAGTCA GTGCGGCGGC
    GGCCCGGGGC TTCTCCAGCT CACACCCGCC CAGCAGTACA GCCTACGGGC CAAGTACGCA
    GCAGCCACAG GTGGACCTCC CCCGACCCCG CTGCCGGGCC TGGAGCGCAC GAGCCTGCGG
    ACCCGGCTGG CGCTGCTGGA TGCTCCCGAG CGCGCGCTGC CCGCTGCCTG CCTGCGCCCG
    ATCGGGCCTC GGCGGGGCAG CGACGGGCCA CACTACGGGC CCTCGGGGCC GTTGCCCGCT
    TTCCCCCACG AGGCACCAGG CGGCGGGGCG CGGCGGGCCA GCGATCCTGG GAGGCGGCCT
    GATGCCCTGG CAGCCCCGCG GGTGCAGCGC TTCCACAGCG CCCTCGACGT GACCCCAGGC
    ACGCTACCGC CACCCTGCGG CCAGGGCCGA GGCCTCCGTC TGCAAAGCCA CACGAGCGCA
    GACGGCAGCC TGGCCCGCAG CGGCTACTCG CCCCGGCCGC CCAGCATCAG CGAGAACGTG
    GTGATGGAAG CCTTGGCGGC GGGGGCGGAC GGCACAGGCC CAGAGGTCAG CCTCGGACTG
    CCAGAGGACG ACCTCGTGCT CCCCGATGAC GTGGTGCAGT ACATCAAGGC ACGTACCAGT
    GGCACCCTGG AGGAGAGTAC CCCACCGGTG TACCCCCCGG AAAGCACCTG CTTCTCTGAG
    AACCCCAAAC TGCCCAGCCC CGGGCTGCAC GGCCAACGTA CCATAGCAGC TGCAGACTCA
    AACGTGGGCC CCTCCGCCCC GGTGCTGGGA GGCTGCCCAC TGGGCTACGG GGCCCCCTCC
    AGCCTGAACA AGAACAGCAT GCCTGTGCAG TGGAACGAAG TGAGCTCAGG CACCGTGGAC
    GCACTGGCCA GCCAGGTGAA GCCTCTGCCC TTCCCACAGG GCAACCTAGC TGTGGTACCA
    CAGAAACCAG CCTTCGCCCA GTACCCAGGA TACAATCCGC AGGGCCTGCA GCCGAGCCCT
    GGGGGCCTAG ACAACTCACA GCCACACTTA CAGCCCTACA GTGGAGCCCC ATCCGCACCC
    AGGGGAAATT ACACACAGCA GCTGCGGCAG CCAGGGGCTG GCAGCCAGTG TCCCAACATG
    ACTGCTGCAG TGAACCCCCA CCCCGGCTAT GGCCAAGCCC ACCCCCAGCT GAGCACCATG
    GGTGGGACCC TGAACCAATT CCCCTCCTCT TGCAGCAACA TGGCAACCAA GCCAGGCCAC
    CTGGGGCTCC CCCAGCAAAT GGAAGTTGCT CCTGACCCCA CCATGATGGG CAACAGCCAC
    AGGGAGCTCG GCATGCCCAA TTCCAGCCTG GCCGGGATGC CACCACCTCA TCCAGCACAG
    AACTACCCAC AGCAGAGTCA TCACCTGGTG ACCACCATGA GCCAGGAGGG CTACCACCAA
    GGCCCCAACC TGGTACCTTC CCACCAACCT AGTTTCATGG AGCCCCAGCA GGGCACAATG
    GGGGCAGCTG GATCTAGCTT TGGCCTAATG CAACCCCGGC CACCCCCAGA GCCTGGCCCT
    GCCGGCCGCC ACCGTGGGGT ACGTGCAGGG CAGCAGCTAG CCTATGCCAG GGCAACTGGC
    CATGCCATGG CTGCTATGTC AGCCAACCAG GAGATGACAG AGTCTGTACC CAAGGGGACA
    ATGGGCAGCA TGTTGTCCCT ACCCCCTCAG CCACCCCCAC AGGACACAGG TGGGACCCAG
    GACCACAGCA TGCTCTACTA CTATGGCCAG ATCCACATGT ACGAGCAGAA CGGAGGCCCT
    GAGAACCAGG CCGGCTGCCA AGCCCTGCGA CCCCAGCCAA CACAGCCCCA GGCCTTGCCC
    TCGCCGGGGG TCAACCAGGT GTCCAGCACT GTGGACTCCC AGCTACTTGA GGCACCCCAG
    ATTGATTTTG ATGCCATCAT GGATGACAGT GATCACTCCA GCTTGCTTTC TGGCGCCCTG
    AGCCCCAGCC TCCTCCACGG CCTCTCCCAG AGCTCCTCCC GCCTCACCAC CCCACGCAGC
    TCCCTGACTC TGCCCTCCAT CCCCGCCGGT ATCAGCAACA TGGCCGTGGG GGACATGAGC
    TCCATGCTCA CCAGCCTGGC CGAGGAGAGC AAGTTCTTGA ACATGATGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015313567.1
    CDS2146..6279
    Translation

    Target ORF information:

    RefSeq Version XM_015458081.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 2 (GLI2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458081.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGACTCTCA TGGCAGGTCA CCCCGCGCCA TATGGGGACC TGCTGATGCA GACTGGGGGT 
    GCGGCCAGTG CACCCCATCT CCATGACTAC CTCAATCCAG TGGATGTGTC ACGTTTCTCC
    AGCCCCCGGG TGACACCCCG CCTGAGCCGC AAGCGGGCGC TGTCCATCTC CCCGCTCTCA
    GACGCCAGCC TCGACCTCCA GCGCATGATC CGGACCTCGC CCAACTCGCT GGTGGCCTAC
    ATCAACAACT CCCGCAGCAG CTCGGCTGCC AGCGGCTCCT ACGGACACCT GTCGGCCGGT
    GCCCTCAGCC CAGCCTTCAC CTTCCCCCAC CCCATCAACC CCGTGGCCTA CCAGCAGATC
    CTGAGCCAGC AGAGGGGCCT GGGCTCAGCC TTCGGACACA CGCCACCCCT GATCCAGCCC
    TCGCCCACCT TCCTGGCCCA GCAGCCAGTG GCCTTCACCT CCATCAATGC CACACCCACC
    CAGCTCAGTA GCAGCAGCAA CTGTCTGAGT GACGCCAATC AGAACAAGCA GAGCAGCGAG
    TCAGCCGTGA GCAGCACTGT CAACCCCATC ATCATTCACA AGCGCAGCAA GGTCAAAACT
    GAGGCTGACG GCCTACGGCC AGCCTCCCCA CTGACCCTAA CACAGGAGCA GCTGGCTGAC
    CTCAAGGAGG ACCTGGAGAG AGACGATTGT AAGCAGGAGG CTGAGGTGGT CATCTATGAG
    ACCAACTGCC ACTGGGAGGA CTGCACCAAG GAGTACGACA CGCAGGAGCA GCTGGTGCAC
    CACATCAACA ACGAGCACAT CCACGGGGAG AAGAAGGAGT TTGTGTGCCG CTGGCAGGCC
    TGCACGCGTG AGCAGAAGCC CTTCAAGGCA CAGTACATGC TTGTGGTGCA CATGCGCAGA
    CACACAGGCG AGAAGCCCCA CAAGTGCACG TTCGAGGGCT GCTCAAAGGC CTATTCCCGC
    CTGGAGAACC TGAAGACTCA CCTGCGCTCC CACACCGGGG AAAAGCCGTA TGTGTGTGAG
    CACGAGGGCT GCAACAAAGC CTTCTCCAAT GCCTCAGACC GCGCCAAGCA CCAGAACCGC
    ACCCACTCCA ACGAGAAACC CTACATCTGC AAGATTCCAG GCTGCACCAA GCGGTACACG
    GACCCCAGCT CCCTCCGGAA GCACGTGAAG ACGGTCCATG GCCCAGACGC TCACGTCACC
    AAGAAACAGC GCAACGATGT GCACCTCCGC GCCCCTCTGC TCCGGGAGAA TGGGGACAGC
    GAGGCCGGTG CCGAGCCCGG CCGGGGCTCC GAGGACAACG CCGAGGCCAG CAGCACTAGC
    CAGGCCGTGG AGGACGGCCT GCACGTCAAG GCCATCAAGA CGGAGCGCTC CGGGCTGTGT
    CAGTCCAGCC CCGGAGCCCA GTCTTCCTGC AGCAGCGAGC CCTCTCCCCT GGGCAGTGCC
    CCCAACAATG ACAGTGGCGT GGAGATGCCG GGGACAGGGC CCGGGAGCCT GGCAGACCTG
    ACAGCTCTGG AGGACCCGCC GCCAGGGGCT GACACCTCCG CCCTGGCCGC CCCCTCTGCT
    GGTGGCCTAC AGCTGCGCAA ACACATGACC GCTATGCACC GGTTCGAGCA GCTCAAGAAG
    GAGAAACTCA AGTCACTGAA GGATTCCTGC TCATGGGCCG GGCCAGCTCC GCACACCCGG
    ACCTCCAAGC TGCCTCCCCT CCCGGGAAGT GGCTCCGTGC TGGAAAACTT CAGCGGCGGC
    GGGGGCGGCG GACCCGCGGG GCTGCTGCCC AACCCGCGGC TGTCTGAGCT GTTCCCGGGC
    GAGGTGACAG TGCTGAGCCA CCTGCAGGAA CGCCGGGACA GCTCCACCAG CACAGTCAGC
    TCGGCCTACA CCGTGAGCCG CCGCTCCTCC GGCATCTCCC CCTACTTCTC TAGTCGCCGC
    TCCAGCGAGG CCTCGCCGCT GGGCGCCGGC CGCGCGCACC CTGCCAGCTC TGCTGATTCC
    TACGACCCCA TCTCCACCGA CGCATCACGG CGCTCGAGTG AGGCCAGTCA GTGCGGCGGC
    GGCCCGGGGC TTCTCCAGCT CACACCCGCC CAGCAGTACA GCCTACGGGC CAAGTACGCA
    GCAGCCACAG GTGGACCTCC CCCGACCCCG CTGCCGGGCC TGGAGCGCAC GAGCCTGCGG
    ACCCGGCTGG CGCTGCTGGA TGCTCCCGAG CGCGCGCTGC CCGCTGCCTG CCTGCGCCCG
    ATCGGGCCTC GGCGGGGCAG CGACGGGCCA CACTACGGGC CCTCGGGGCC GTTGCCCGCT
    TTCCCCCACG AGGCACCAGG CGGCGGGGCG CGGCGGGCCA GCGATCCTGG GAGGCGGCCT
    GATGCCCTGG CAGCCCCGCG GGTGCAGCGC TTCCACAGCG CCCTCGACGT GACCCCAGGC
    ACGCTACCGC CACCCTGCGG CCAGGGCCGA GGCCTCCGTC TGCAAAGCCA CACGAGCGCA
    GACGGCAGCC TGGCCCGCAG CGGCTACTCG CCCCGGCCGC CCAGCATCAG CGAGAACGTG
    GTGATGGAAG CCTTGGCGGC GGGGGCGGAC GGCACAGGCC CAGAGGTCAG CCTCGGACTG
    CCAGAGGACG ACCTCGTGCT CCCCGATGAC GTGGTGCAGT ACATCAAGGC ACGTACCAGT
    GGCACCCTGG AGGAGAGTAC CCCACCGGTG TACCCCCCGG AAAGCACCTG CTTCTCTGAG
    AACCCCAAAC TGCCCAGCCC CGGGCTGCAC GGCCAACGTA CCATAGCAGC TGCAGACTCA
    AACGTGGGCC CCTCCGCCCC GGTGCTGGGA GGCTGCCCAC TGGGCTACGG GGCCCCCTCC
    AGCCTGAACA AGAACAGCAT GCCTGTGCAG TGGAACGAAG TGAGCTCAGG CACCGTGGAC
    GCACTGGCCA GCCAGGTGAA GCCTCTGCCC TTCCCACAGG GCAACCTAGC TGTGGTACCA
    CAGAAACCAG CCTTCGCCCA GTACCCAGGA TACAATCCGC AGGGCCTGCA GCCGAGCCCT
    GGGGGCCTAG ACAACTCACA GCCACACTTA CAGCCCTACA GTGGAGCCCC ATCCGCACCC
    AGGGGAAATT ACACACAGCA GCTGCGGCAG CCAGGGGCTG GCAGCCAGTG TCCCAACATG
    ACTGCTGCAG TGAACCCCCA CCCCGGCTAT GGCCAAGCCC ACCCCCAGCT GAGCACCATG
    GGTGGGACCC TGAACCAATT CCCCTCCTCT TGCAGCAACA TGGCAACCAA GCCAGGCCAC
    CTGGGGCTCC CCCAGCAAAT GGAAGTTGCT CCTGACCCCA CCATGATGGG CAACAGCCAC
    AGGGAGCTCG GCATGCCCAA TTCCAGCCTG GCCGGGATGC CACCACCTCA TCCAGCACAG
    AACTACCCAC AGCAGAGTCA TCACCTGGTG ACCACCATGA GCCAGGAGGG CTACCACCAA
    GGCCCCAACC TGGTACCTTC CCACCAACCT AGTTTCATGG AGCCCCAGCA GGGCACAATG
    GGGGCAGCTG GATCTAGCTT TGGCCTAATG CAACCCCGGC CACCCCCAGA GCCTGGCCCT
    GCCGGCCGCC ACCGTGGGGT ACGTGCAGGG CAGCAGCTAG CCTATGCCAG GGCAACTGGC
    CATGCCATGG CTGCTATGTC AGCCAACCAG GAGATGACAG AGTCTGTACC CAAGGGGACA
    ATGGGCAGCA TGTTGTCCCT ACCCCCTCAG CCACCCCCAC AGGACACAGG TGGGACCCAG
    GACCACAGCA TGCTCTACTA CTATGGCCAG ATCCACATGT ACGAGCAGAA CGGAGGCCCT
    GAGAACCAGG CCGGCTGCCA AGCCCTGCGA CCCCAGCCAA CACAGCCCCA GGCCTTGCCC
    TCGCCGGGGG TCAACCAGGT GTCCAGCACT GTGGACTCCC AGCTACTTGA GGCACCCCAG
    ATTGATTTTG ATGCCATCAT GGATGACAGT GATCACTCCA GCTTGCTTTC TGGCGCCCTG
    AGCCCCAGCC TCCTCCACGG CCTCTCCCAG AGCTCCTCCC GCCTCACCAC CCCACGCAGC
    TCCCTGACTC TGCCCTCCAT CCCCGCCGGT ATCAGCAACA TGGCCGTGGG GGACATGAGC
    TCCATGCTCA CCAGCCTGGC CGAGGAGAGC AAGTTCTTGA ACATGATGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.