gRNAs
The following GLI2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLI2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OBa37605 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001192250.2 | |
| Accession Version | NM_001192250.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-29 | |
| Organism | Bos taurus(cattle) | |
| Product | zinc finger protein GLI2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000002.1. On Feb 13, 2012 this sequence version replaced NM_001192250.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145422, SAMN03145425 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGAGACAT CCGCCTCAGC CACGGCCACG GACAAGAAAG AAGTCAAAGG TGGGATCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001179179.2 |
| CDS | 1..4773 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001192250.2 |
1 | ATGGAGACAT CCGCCTCAGC CACGGCCACG GACAAGAAAG AAGTCAAAGG TGGGATCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa87936 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015458080.1 , XM_015458080.2 | |
| Accession Version | XM_015458080.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4674bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-25 | |
| Organism | Bos taurus(cattle) | |
| Product | zinc finger protein GLI2 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103843.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGACAT CCGCCTCAGC CACGGCCACG GACAAGAAAG AAGTCAAAGG TGGGATCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015313566.1 |
| CDS | 57..4730 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015458080.1 |
1 | ATGGAGACAT CCGCCTCAGC CACGGCCACG GACAAGAAAG AAGTCAAAGG TGGGATCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa87936 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015458080.1 , XM_015458080.2 | |
| Accession Version | XM_015458080.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4674bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | zinc finger protein GLI2 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGACAT CCGCCTCAGC CACGGCCACG GACAAGAAAG AAGTCAAAGG TGGGATCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015313566.1 |
| CDS | 55..4728 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015458080.2 |
1 | ATGGAGACAT CCGCCTCAGC CACGGCCACG GACAAGAAAG AAGTCAAAGG TGGGATCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa87937 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015458081.1 | |
| Accession Version | XM_015458081.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4134bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-25 | |
| Organism | Bos taurus(cattle) | |
| Product | zinc finger protein GLI2 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103843.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGACTCTCA TGGCAGGTCA CCCCGCGCCA TATGGGGACC TGCTGATGCA GACTGGGGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015313567.1 |
| CDS | 2146..6279 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015458081.1 |
1 | ATGACTCTCA TGGCAGGTCA CCCCGCGCCA TATGGGGACC TGCTGATGCA GACTGGGGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Expression analysis of Gli1 and Gli2 in different tissues and muscle-derived cells of Qinchuan cattle. |
A whole-genome assembly of the domestic cow, Bos taurus. |
