GLI2 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_005270.4	NP_005261.2	zinc finger protein GLI2
NM_001371271.1	NP_001358200.1	zinc finger protein GLI2 isoform 1
NM_001374353.1	NP_001361282.1	zinc finger protein GLI2 isoform 2
XM_011510974.2	XP_011509276.1	zinc finger protein GLI2 isoform X9
XM_024452795.1	XP_024308563.1	zinc finger protein GLI2 isoform X3
XM_011510969.2	XP_011509271.2	zinc finger protein GLI2 isoform X1
XM_017003818.1	XP_016859307.1	zinc finger protein GLI2 isoform X2
NM_001374354.1	NP_001361283.1	zinc finger protein GLI2 isoform 3
XM_006712422.2	XP_006712485.1	zinc finger protein GLI2 isoform X3
XM_011510970.2	XP_011509272.1	zinc finger protein GLI2 isoform X6
XM_011510973.2	XP_011509275.1	zinc finger protein GLI2 isoform X8
XM_011510971.2	XP_011509273.1	zinc finger protein GLI2 isoform X7
NM_005270.5	NP_005261.2	zinc finger protein GLI2 isoform 1
XM_006712422.3	XP_006712485.1	zinc finger protein GLI2 isoform X4
XM_024452794.1	XP_024308562.1	zinc finger protein GLI2 isoform X3
XM_011510972.2	XP_011509274.2	zinc finger protein GLI2 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28661	NM_005270.5 Latest version!	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu28661	NM_001371271.1 Latest version!	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu28661	XM_024452795.1 Latest version!	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu28661	XM_024452794.1 Latest version!	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu57986	NM_001374354.1 Latest version!	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu36103	NM_001374353.1 Latest version!	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu36103	XM_006712422.3 Latest version!	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu57983	XM_011510970.2 Latest version!	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu57984	XM_011510971.2 Latest version!	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu57985	XM_011510973.2 Latest version!	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu57986	XM_011510974.2 Latest version!	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu89279	XM_011510969.2 Latest version!	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu89280	XM_017003818.1 Latest version!	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu89281	XM_011510972.2 Latest version!	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
View Old Accession Versions >>
OHu28661	NM_005270.4	Homo sapiens GLI family zinc finger 2 (GLI2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu36103	XM_006712422.2	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
Hide Old Accession Versions >>

GLI2 ( NM_005270.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005261.2 Homo sapiens GLI family zinc finger 2 (GLI2), mRNA.

Price & Availability↑

CloneID	OHu28661
Clone ID Related Accession (Same CDS sequence)	NM_005270.4 , NM_001371271.1 , XM_024452795.1 , NM_005270.5 , XM_024452794.1
Accession Version	NM_005270.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	zinc finger protein GLI2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ004398.1, DQ086814.1, AB209354.1 and AJ707583.1. This sequence is a reference standard in the RefSeqGene project. On Jun 13, 2008 this sequence version replaced NM_005270.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ086814.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG 
GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG 
GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG 
CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC 
CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG 
ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG 
TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC 
ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG 
AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG 
ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT 
GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC 
CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC 
GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC 
AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC 
CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG 
AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA 
CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG 
CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG 
GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG 
CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGGCCAGGT GTCTGGACAC 
GGCTCATGTG GGTGTGCCCT TCCCCTCTCC CAGGAGCAGC TGGCTGACCT CAAGGAAGAT 
CTGGACAGGG ATGACTGTAA GCAGGAGGCT GAGGTGGTCA TCTATGAGAC CAACTGCCAC 
TGGGAAGACT GCACCAAGGA GTACGACACC CAGGAGCAGC TGGTGCATCA CATCAACAAC 
GAGCACATCC ACGGGGAGAA GAAGGAGTTT GTGTGCCGCT GGCAGGCCTG CACGCGGGAG 
CAGAAGCCCT TCAAGGCGCA GTACATGCTG GTGGTGCACA TGCGGCGACA CACGGGCGAG 
AAGCCCCACA AGTGCACGTT CGAGGGCTGC TCGAAGGCCT ACTCCCGCCT GGAGAACCTG 
AAGACACACC TGCGGTCCCA CACCGGGGAG AAGCCATATG TGTGTGAGCA CGAGGGCTGC 
AACAAAGCCT TCTCCAACGC CTCGGACCGC GCCAAGCACC AGAATCGCAC CCACTCCAAC 
GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GATACACAGA CCCCAGCTCT 
CTCCGGAAGC ATGTGAAAAC GGTCCACGGC CCAGATGCCC ACGTCACCAA GAAGCAGCGC 
AATGACGTGC ACCTCCGCAC ACCGCTGCTC AAAGAGAATG GGGACAGTGA GGCCGGCACG 
GAGCCTGGCG GCCCAGAGAG CACCGAGGCC AGCAGCACCA GCCAGGCCGT GGAGGACTGC 
CTGCACGTCA GAGCCATCAA GACCGAGAGC TCCGGGCTGT GTCAGTCCAG CCCCGGGGCC 
CAGTCGTCCT GCAGCAGCGA GCCCTCTCCT CTGGGCAGTG CCCCCAACAA TGACAGTGGC 
GTGGAGATGC CGGGGACGGG GCCCGGGAGC CTGGGAGACC TGACGGCACT GGATGACACA 
CCCCCAGGGG CCGACACCTC AGCCCTGGCT GCCCCCTCCG CTGGTGGCCT CCAGCTGCGC 
AAACACATGA CCACCATGCA CCGGTTCGAG CAGCTCAAGA AGGAGAAGCT CAAGTCACTC 
AAGGATTCCT GCTCATGGGC CGGGCCGACT CCACACACGC GGAACACCAA GCTGCCTCCC 
CTCCCGGGAA GTGGCTCCAT CCTGGAAAAC TTCAGTGGCA GTGGGGGCGG CGGGCCCGCG 
GGGCTGCTGC CGAACCCGCG GCTGTCGGAG CTGTCCGCGA GCGAGGTGAC CATGCTGAGC 
CAGCTGCAGG AGCGCCGCGA CAGCTCCACC AGCACGGTCA GCTCGGCCTA CACCGTGAGC 
CGCCGCTCCT CCGGCATCTC CCCCTACTTC TCCAGCCGCC GCTCCAGCGA GGCCTCGCCC 
CTGGGCGCCG GCCGCCCGCA CAACGCGAGC TCCGCTGACT CCTACGACCC CATCTCCACG 
GACGCGTCGC GGCGCTCGAG CGAGGCCAGC CAGTGCAGCG GCGGCTCCGG GCTGCTCAAC 
CTCACGCCGG CGCAGCAGTA CAGCCTGCGG GCCAAGTACG CGGCAGCCAC TGGCGGCCCC 
CCGCCCACTC CGCTGCCGGG CCTGGAGCGC ATGAGCCTGC GGACCAGGCT GGCGCTGCTG 
GACGCGCCCG AGCGCACGCT GCCCGCCGGC TGCCCACGCC CACTGGGGCC GCGGCGTGGC 
AGCGACGGGC CGACCTATGG CCACGGCCAC GCGGGGGCTG CGCCCGCCTT CCCCCACGAG 
GCTCCAGGCG GCGGAGCCAG GCGGGCCAGC GACCCTGTGC GGCGGCCCGA TGCCCTGTCC 
CTGCCGCGGG TGCAGCGCTT CCACAGCACC CACAACGTGA ACCCCGGCCC GCTGCCGCCC 
TGTGCCGACA GGCGAGGCCT CCGCCTGCAG AGCCACCCGA GCACCGACGG CGGCCTGGCC 
CGCGGCGCCT ACTCGCCCCG GCCGCCTAGC ATCAGCGAGA ACGTGGCGAT GGAGGCCGTG 
GCGGCAGGAG TGGACGGCGC GGGGCCCGAG GCCGACCTGG GGCTGCCGGA GGACGACCTG 
GTGCTTCCAG ACGACGTGGT GCAGTACATC AAGGCGCACG CCAGTGGCGC TCTGGACGAG 
GGCACCGGGC AGGTGTATCC CACGGAAAGC ACTGGCTTCT CTGACAACCC CAGACTACCC 
AGCCCGGGGC TGCACGGCCA GCGCAGGATG GTGGCTGCGG ACTCCAACGT GGGCCCCTCC 
GCCCCTATGC TGGGAGGATG CCAGTTAGGC TTTGGGGCGC CCTCCAGCCT GAACAAAAAT 
AACATGCCTG TGCAGTGGAA TGAGGTGAGC TCCGGCACCG TAGACGCCCT GGCCAGCCAG 
GTGAAGCCTC CACCCTTTCC TCAGGGCAAC CTGGCGGTGG TGCAGCAGAA GCCTGCCTTT 
GGCCAGTACC CGGGCTACAG TCCGCAAGGC CTACAGGCTA GCCCTGGGGG CCTGGACAGC 
ACGCAGCCAC ACCTGCAGCC CCGCAGCGGA GCCCCCTCCC AGGGCATCCC CAGGGTAAAC 
TACATGCAGC AGCTGCGACA GCCAGTGGCA GGCAGCCAGT GTCCTGGCAT GACTACCACT 
ATGAGCCCCC ATGCCTGCTA TGGCCAAGTC CACCCCCAGC TGAGCCCCAG CACCATCAGT 
GGGGCCCTCA ACCAGTTCCC CCAATCCTGC AGCAACATGC CAGCCAAGCC AGGGCATCTG 
GGGCACCCTC AGCAGACAGA AGTGGCACCT GACCCCACCA CGATGGGCAA TCGCCACAGG 
GAACTTGGGG TCCCCGATTC AGCCCTGGCT GGAGTGCCAC CACCTCACCC AGTCCAGAGC 
TACCCACAGC AGAGCCATCA CCTGGCAGCC TCCATGAGCC AGGAGGGCTA CCACCAGGTC 
CCCAGCCTTC TGCCTGCCCG CCAGCCTGGC TTCATGGAGC CCCAAACAGG CCCGATGGGG 
GTGGCTACAG CAGGCTTTGG CCTAGTGCAG CCCCGGCCTC CCCTCGAGCC CAGCCCCACT 
GGCCGCCACC GTGGGGTACG TGCTGTGCAG CAGCAGCTGG CCTACGCCAG GGCCACAGGC 
CATGCCATGG CTGCCATGCC GTCCAGTCAG GAAACAGCAG AGGCTGTGCC CAAGGGAGCG 
ATGGGCAACA TGGGGTCGGT GCCTCCCCAG CCGCCTCCGC AGGACGCAGG TGGGGCCCCG 
GACCACAGCA TGCTCTACTA CTACGGCCAG ATCCACATGT ACGAACAGGA TGGAGGCCTG 
GAGAACCTCG GGAGCTGCCA GGTCATGCGG TCCCAGCCAC CACAGCCACA GGCCTGTCAG 
GACAGCATCC AGCCCCAGCC CTTGCCCTCA CCAGGGGTCA ACCAGGTGTC CAGCACTGTG 
GACTCCCAGC TCCTGGAGGC CCCCCAGATT GACTTCGATG CCATCATGGA TGATGGCGAT 
CACTCGAGTT TGTTCTCGGG TGCTCTGAGC CCCAGCCTCC TCCACAGCCT CTCCCAGAAC 
TCCTCCCGCC TCACCACCCC CCGAAACTCC TTGACCCTGC CCTCCATCCC CGCAGGCATC 
AGCAACATGG CTGTCGGGGA CATGAGCTCC ATGCTCACCA GCCTCGCCGA GGAGAGCAAG 
TTCCTGAACA TGATGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005261.2
CDS	31..4791
Translation	METSASATASEKQEAKSGILEAAGFPDPGKKASPLVVAAAAAAAVAAQGVPQHLLPPFHAPLPIDMRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQGQVSGHGSCGCALPLSQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	NM_005270.4
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 2 (GLI2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005270.4

ORF Insert Sequence:

ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG 
GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG 
GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG 
CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC 
CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG 
ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG 
TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC 
ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG 
AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG 
ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT 
GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC 
CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC 
GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC 
AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC 
CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG 
AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA 
CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG 
CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG 
GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG 
CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGGCCAGGT GTCTGGACAC 
GGCTCATGTG GGTGTGCCCT TCCCCTCTCC CAGGAGCAGC TGGCTGACCT CAAGGAAGAT 
CTGGACAGGG ATGACTGTAA GCAGGAGGCT GAGGTGGTCA TCTATGAGAC CAACTGCCAC 
TGGGAAGACT GCACCAAGGA GTACGACACC CAGGAGCAGC TGGTGCATCA CATCAACAAC 
GAGCACATCC ACGGGGAGAA GAAGGAGTTT GTGTGCCGCT GGCAGGCCTG CACGCGGGAG 
CAGAAGCCCT TCAAGGCGCA GTACATGCTG GTGGTGCACA TGCGGCGACA CACGGGCGAG 
AAGCCCCACA AGTGCACGTT CGAGGGCTGC TCGAAGGCCT ACTCCCGCCT GGAGAACCTG 
AAGACACACC TGCGGTCCCA CACCGGGGAG AAGCCATATG TGTGTGAGCA CGAGGGCTGC 
AACAAAGCCT TCTCCAACGC CTCGGACCGC GCCAAGCACC AGAATCGCAC CCACTCCAAC 
GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GATACACAGA CCCCAGCTCT 
CTCCGGAAGC ATGTGAAAAC GGTCCACGGC CCAGATGCCC ACGTCACCAA GAAGCAGCGC 
AATGACGTGC ACCTCCGCAC ACCGCTGCTC AAAGAGAATG GGGACAGTGA GGCCGGCACG 
GAGCCTGGCG GCCCAGAGAG CACCGAGGCC AGCAGCACCA GCCAGGCCGT GGAGGACTGC 
CTGCACGTCA GAGCCATCAA GACCGAGAGC TCCGGGCTGT GTCAGTCCAG CCCCGGGGCC 
CAGTCGTCCT GCAGCAGCGA GCCCTCTCCT CTGGGCAGTG CCCCCAACAA TGACAGTGGC 
GTGGAGATGC CGGGGACGGG GCCCGGGAGC CTGGGAGACC TGACGGCACT GGATGACACA 
CCCCCAGGGG CCGACACCTC AGCCCTGGCT GCCCCCTCCG CTGGTGGCCT CCAGCTGCGC 
AAACACATGA CCACCATGCA CCGGTTCGAG CAGCTCAAGA AGGAGAAGCT CAAGTCACTC 
AAGGATTCCT GCTCATGGGC CGGGCCGACT CCACACACGC GGAACACCAA GCTGCCTCCC 
CTCCCGGGAA GTGGCTCCAT CCTGGAAAAC TTCAGTGGCA GTGGGGGCGG CGGGCCCGCG 
GGGCTGCTGC CGAACCCGCG GCTGTCGGAG CTGTCCGCGA GCGAGGTGAC CATGCTGAGC 
CAGCTGCAGG AGCGCCGCGA CAGCTCCACC AGCACGGTCA GCTCGGCCTA CACCGTGAGC 
CGCCGCTCCT CCGGCATCTC CCCCTACTTC TCCAGCCGCC GCTCCAGCGA GGCCTCGCCC 
CTGGGCGCCG GCCGCCCGCA CAACGCGAGC TCCGCTGACT CCTACGACCC CATCTCCACG 
GACGCGTCGC GGCGCTCGAG CGAGGCCAGC CAGTGCAGCG GCGGCTCCGG GCTGCTCAAC 
CTCACGCCGG CGCAGCAGTA CAGCCTGCGG GCCAAGTACG CGGCAGCCAC TGGCGGCCCC 
CCGCCCACTC CGCTGCCGGG CCTGGAGCGC ATGAGCCTGC GGACCAGGCT GGCGCTGCTG 
GACGCGCCCG AGCGCACGCT GCCCGCCGGC TGCCCACGCC CACTGGGGCC GCGGCGTGGC 
AGCGACGGGC CGACCTATGG CCACGGCCAC GCGGGGGCTG CGCCCGCCTT CCCCCACGAG 
GCTCCAGGCG GCGGAGCCAG GCGGGCCAGC GACCCTGTGC GGCGGCCCGA TGCCCTGTCC 
CTGCCGCGGG TGCAGCGCTT CCACAGCACC CACAACGTGA ACCCCGGCCC GCTGCCGCCC 
TGTGCCGACA GGCGAGGCCT CCGCCTGCAG AGCCACCCGA GCACCGACGG CGGCCTGGCC 
CGCGGCGCCT ACTCGCCCCG GCCGCCTAGC ATCAGCGAGA ACGTGGCGAT GGAGGCCGTG 
GCGGCAGGAG TGGACGGCGC GGGGCCCGAG GCCGACCTGG GGCTGCCGGA GGACGACCTG 
GTGCTTCCAG ACGACGTGGT GCAGTACATC AAGGCGCACG CCAGTGGCGC TCTGGACGAG 
GGCACCGGGC AGGTGTATCC CACGGAAAGC ACTGGCTTCT CTGACAACCC CAGACTACCC 
AGCCCGGGGC TGCACGGCCA GCGCAGGATG GTGGCTGCGG ACTCCAACGT GGGCCCCTCC 
GCCCCTATGC TGGGAGGATG CCAGTTAGGC TTTGGGGCGC CCTCCAGCCT GAACAAAAAT 
AACATGCCTG TGCAGTGGAA TGAGGTGAGC TCCGGCACCG TAGACGCCCT GGCCAGCCAG 
GTGAAGCCTC CACCCTTTCC TCAGGGCAAC CTGGCGGTGG TGCAGCAGAA GCCTGCCTTT 
GGCCAGTACC CGGGCTACAG TCCGCAAGGC CTACAGGCTA GCCCTGGGGG CCTGGACAGC 
ACGCAGCCAC ACCTGCAGCC CCGCAGCGGA GCCCCCTCCC AGGGCATCCC CAGGGTAAAC 
TACATGCAGC AGCTGCGACA GCCAGTGGCA GGCAGCCAGT GTCCTGGCAT GACTACCACT 
ATGAGCCCCC ATGCCTGCTA TGGCCAAGTC CACCCCCAGC TGAGCCCCAG CACCATCAGT 
GGGGCCCTCA ACCAGTTCCC CCAATCCTGC AGCAACATGC CAGCCAAGCC AGGGCATCTG 
GGGCACCCTC AGCAGACAGA AGTGGCACCT GACCCCACCA CGATGGGCAA TCGCCACAGG 
GAACTTGGGG TCCCCGATTC AGCCCTGGCT GGAGTGCCAC CACCTCACCC AGTCCAGAGC 
TACCCACAGC AGAGCCATCA CCTGGCAGCC TCCATGAGCC AGGAGGGCTA CCACCAGGTC 
CCCAGCCTTC TGCCTGCCCG CCAGCCTGGC TTCATGGAGC CCCAAACAGG CCCGATGGGG 
GTGGCTACAG CAGGCTTTGG CCTAGTGCAG CCCCGGCCTC CCCTCGAGCC CAGCCCCACT 
GGCCGCCACC GTGGGGTACG TGCTGTGCAG CAGCAGCTGG CCTACGCCAG GGCCACAGGC 
CATGCCATGG CTGCCATGCC GTCCAGTCAG GAAACAGCAG AGGCTGTGCC CAAGGGAGCG 
ATGGGCAACA TGGGGTCGGT GCCTCCCCAG CCGCCTCCGC AGGACGCAGG TGGGGCCCCG 
GACCACAGCA TGCTCTACTA CTACGGCCAG ATCCACATGT ACGAACAGGA TGGAGGCCTG 
GAGAACCTCG GGAGCTGCCA GGTCATGCGG TCCCAGCCAC CACAGCCACA GGCCTGTCAG 
GACAGCATCC AGCCCCAGCC CTTGCCCTCA CCAGGGGTCA ACCAGGTGTC CAGCACTGTG 
GACTCCCAGC TCCTGGAGGC CCCCCAGATT GACTTCGATG CCATCATGGA TGATGGCGAT 
CACTCGAGTT TGTTCTCGGG TGCTCTGAGC CCCAGCCTCC TCCACAGCCT CTCCCAGAAC 
TCCTCCCGCC TCACCACCCC CCGAAACTCC TTGACCCTGC CCTCCATCCC CGCAGGCATC 
AGCAACATGG CTGTCGGGGA CATGAGCTCC ATGCTCACCA GCCTCGCCGA GGAGAGCAAG 
TTCCTGAACA TGATGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( NM_001371271.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001358200.1 Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant 1, mRNA.

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CloneID	OHu28661
Clone ID Related Accession (Same CDS sequence)	NM_005270.4 , NM_001371271.1 , XM_024452795.1 , NM_005270.5 , XM_024452794.1
Accession Version	NM_001371271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	zinc finger protein GLI2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC017033.5 and AC016764.8. On Jul 6, 2019 this sequence version replaced XM_024452794.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: DQ086814.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG 
GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG 
GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG 
CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC 
CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG 
ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG 
TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC 
ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG 
AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG 
ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT 
GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC 
CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC 
GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC 
AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC 
CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG 
AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA 
CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG 
CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG 
GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG 
CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGGCCAGGT GTCTGGACAC 
GGCTCATGTG GGTGTGCCCT TCCCCTCTCC CAGGAGCAGC TGGCTGACCT CAAGGAAGAT 
CTGGACAGGG ATGACTGTAA GCAGGAGGCT GAGGTGGTCA TCTATGAGAC CAACTGCCAC 
TGGGAAGACT GCACCAAGGA GTACGACACC CAGGAGCAGC TGGTGCATCA CATCAACAAC 
GAGCACATCC ACGGGGAGAA GAAGGAGTTT GTGTGCCGCT GGCAGGCCTG CACGCGGGAG 
CAGAAGCCCT TCAAGGCGCA GTACATGCTG GTGGTGCACA TGCGGCGACA CACGGGCGAG 
AAGCCCCACA AGTGCACGTT CGAGGGCTGC TCGAAGGCCT ACTCCCGCCT GGAGAACCTG 
AAGACACACC TGCGGTCCCA CACCGGGGAG AAGCCATATG TGTGTGAGCA CGAGGGCTGC 
AACAAAGCCT TCTCCAACGC CTCGGACCGC GCCAAGCACC AGAATCGCAC CCACTCCAAC 
GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GATACACAGA CCCCAGCTCT 
CTCCGGAAGC ATGTGAAAAC GGTCCACGGC CCAGATGCCC ACGTCACCAA GAAGCAGCGC 
AATGACGTGC ACCTCCGCAC ACCGCTGCTC AAAGAGAATG GGGACAGTGA GGCCGGCACG 
GAGCCTGGCG GCCCAGAGAG CACCGAGGCC AGCAGCACCA GCCAGGCCGT GGAGGACTGC 
CTGCACGTCA GAGCCATCAA GACCGAGAGC TCCGGGCTGT GTCAGTCCAG CCCCGGGGCC 
CAGTCGTCCT GCAGCAGCGA GCCCTCTCCT CTGGGCAGTG CCCCCAACAA TGACAGTGGC 
GTGGAGATGC CGGGGACGGG GCCCGGGAGC CTGGGAGACC TGACGGCACT GGATGACACA 
CCCCCAGGGG CCGACACCTC AGCCCTGGCT GCCCCCTCCG CTGGTGGCCT CCAGCTGCGC 
AAACACATGA CCACCATGCA CCGGTTCGAG CAGCTCAAGA AGGAGAAGCT CAAGTCACTC 
AAGGATTCCT GCTCATGGGC CGGGCCGACT CCACACACGC GGAACACCAA GCTGCCTCCC 
CTCCCGGGAA GTGGCTCCAT CCTGGAAAAC TTCAGTGGCA GTGGGGGCGG CGGGCCCGCG 
GGGCTGCTGC CGAACCCGCG GCTGTCGGAG CTGTCCGCGA GCGAGGTGAC CATGCTGAGC 
CAGCTGCAGG AGCGCCGCGA CAGCTCCACC AGCACGGTCA GCTCGGCCTA CACCGTGAGC 
CGCCGCTCCT CCGGCATCTC CCCCTACTTC TCCAGCCGCC GCTCCAGCGA GGCCTCGCCC 
CTGGGCGCCG GCCGCCCGCA CAACGCGAGC TCCGCTGACT CCTACGACCC CATCTCCACG 
GACGCGTCGC GGCGCTCGAG CGAGGCCAGC CAGTGCAGCG GCGGCTCCGG GCTGCTCAAC 
CTCACGCCGG CGCAGCAGTA CAGCCTGCGG GCCAAGTACG CGGCAGCCAC TGGCGGCCCC 
CCGCCCACTC CGCTGCCGGG CCTGGAGCGC ATGAGCCTGC GGACCAGGCT GGCGCTGCTG 
GACGCGCCCG AGCGCACGCT GCCCGCCGGC TGCCCACGCC CACTGGGGCC GCGGCGTGGC 
AGCGACGGGC CGACCTATGG CCACGGCCAC GCGGGGGCTG CGCCCGCCTT CCCCCACGAG 
GCTCCAGGCG GCGGAGCCAG GCGGGCCAGC GACCCTGTGC GGCGGCCCGA TGCCCTGTCC 
CTGCCGCGGG TGCAGCGCTT CCACAGCACC CACAACGTGA ACCCCGGCCC GCTGCCGCCC 
TGTGCCGACA GGCGAGGCCT CCGCCTGCAG AGCCACCCGA GCACCGACGG CGGCCTGGCC 
CGCGGCGCCT ACTCGCCCCG GCCGCCTAGC ATCAGCGAGA ACGTGGCGAT GGAGGCCGTG 
GCGGCAGGAG TGGACGGCGC GGGGCCCGAG GCCGACCTGG GGCTGCCGGA GGACGACCTG 
GTGCTTCCAG ACGACGTGGT GCAGTACATC AAGGCGCACG CCAGTGGCGC TCTGGACGAG 
GGCACCGGGC AGGTGTATCC CACGGAAAGC ACTGGCTTCT CTGACAACCC CAGACTACCC 
AGCCCGGGGC TGCACGGCCA GCGCAGGATG GTGGCTGCGG ACTCCAACGT GGGCCCCTCC 
GCCCCTATGC TGGGAGGATG CCAGTTAGGC TTTGGGGCGC CCTCCAGCCT GAACAAAAAT 
AACATGCCTG TGCAGTGGAA TGAGGTGAGC TCCGGCACCG TAGACGCCCT GGCCAGCCAG 
GTGAAGCCTC CACCCTTTCC TCAGGGCAAC CTGGCGGTGG TGCAGCAGAA GCCTGCCTTT 
GGCCAGTACC CGGGCTACAG TCCGCAAGGC CTACAGGCTA GCCCTGGGGG CCTGGACAGC 
ACGCAGCCAC ACCTGCAGCC CCGCAGCGGA GCCCCCTCCC AGGGCATCCC CAGGGTAAAC 
TACATGCAGC AGCTGCGACA GCCAGTGGCA GGCAGCCAGT GTCCTGGCAT GACTACCACT 
ATGAGCCCCC ATGCCTGCTA TGGCCAAGTC CACCCCCAGC TGAGCCCCAG CACCATCAGT 
GGGGCCCTCA ACCAGTTCCC CCAATCCTGC AGCAACATGC CAGCCAAGCC AGGGCATCTG 
GGGCACCCTC AGCAGACAGA AGTGGCACCT GACCCCACCA CGATGGGCAA TCGCCACAGG 
GAACTTGGGG TCCCCGATTC AGCCCTGGCT GGAGTGCCAC CACCTCACCC AGTCCAGAGC 
TACCCACAGC AGAGCCATCA CCTGGCAGCC TCCATGAGCC AGGAGGGCTA CCACCAGGTC 
CCCAGCCTTC TGCCTGCCCG CCAGCCTGGC TTCATGGAGC CCCAAACAGG CCCGATGGGG 
GTGGCTACAG CAGGCTTTGG CCTAGTGCAG CCCCGGCCTC CCCTCGAGCC CAGCCCCACT 
GGCCGCCACC GTGGGGTACG TGCTGTGCAG CAGCAGCTGG CCTACGCCAG GGCCACAGGC 
CATGCCATGG CTGCCATGCC GTCCAGTCAG GAAACAGCAG AGGCTGTGCC CAAGGGAGCG 
ATGGGCAACA TGGGGTCGGT GCCTCCCCAG CCGCCTCCGC AGGACGCAGG TGGGGCCCCG 
GACCACAGCA TGCTCTACTA CTACGGCCAG ATCCACATGT ACGAACAGGA TGGAGGCCTG 
GAGAACCTCG GGAGCTGCCA GGTCATGCGG TCCCAGCCAC CACAGCCACA GGCCTGTCAG 
GACAGCATCC AGCCCCAGCC CTTGCCCTCA CCAGGGGTCA ACCAGGTGTC CAGCACTGTG 
GACTCCCAGC TCCTGGAGGC CCCCCAGATT GACTTCGATG CCATCATGGA TGATGGCGAT 
CACTCGAGTT TGTTCTCGGG TGCTCTGAGC CCCAGCCTCC TCCACAGCCT CTCCCAGAAC 
TCCTCCCGCC TCACCACCCC CCGAAACTCC TTGACCCTGC CCTCCATCCC CGCAGGCATC 
AGCAACATGG CTGTCGGGGA CATGAGCTCC ATGCTCACCA GCCTCGCCGA GGAGAGCAAG 
TTCCTGAACA TGATGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001358200.1
CDS	449..5209
Translation	METSASATASEKQEAKSGILEAAGFPDPGKKASPLVVAAAAAAAVAAQGVPQHLLPPFHAPLPIDMRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQGQVSGHGSCGCALPLSQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	NM_001371271.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371271.1

ORF Insert Sequence:

ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG 
GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG 
GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG 
CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC 
CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG 
ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG 
TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC 
ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG 
AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG 
ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT 
GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC 
CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC 
GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC 
AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC 
CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG 
AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA 
CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG 
CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG 
GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG 
CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGGCCAGGT GTCTGGACAC 
GGCTCATGTG GGTGTGCCCT TCCCCTCTCC CAGGAGCAGC TGGCTGACCT CAAGGAAGAT 
CTGGACAGGG ATGACTGTAA GCAGGAGGCT GAGGTGGTCA TCTATGAGAC CAACTGCCAC 
TGGGAAGACT GCACCAAGGA GTACGACACC CAGGAGCAGC TGGTGCATCA CATCAACAAC 
GAGCACATCC ACGGGGAGAA GAAGGAGTTT GTGTGCCGCT GGCAGGCCTG CACGCGGGAG 
CAGAAGCCCT TCAAGGCGCA GTACATGCTG GTGGTGCACA TGCGGCGACA CACGGGCGAG 
AAGCCCCACA AGTGCACGTT CGAGGGCTGC TCGAAGGCCT ACTCCCGCCT GGAGAACCTG 
AAGACACACC TGCGGTCCCA CACCGGGGAG AAGCCATATG TGTGTGAGCA CGAGGGCTGC 
AACAAAGCCT TCTCCAACGC CTCGGACCGC GCCAAGCACC AGAATCGCAC CCACTCCAAC 
GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GATACACAGA CCCCAGCTCT 
CTCCGGAAGC ATGTGAAAAC GGTCCACGGC CCAGATGCCC ACGTCACCAA GAAGCAGCGC 
AATGACGTGC ACCTCCGCAC ACCGCTGCTC AAAGAGAATG GGGACAGTGA GGCCGGCACG 
GAGCCTGGCG GCCCAGAGAG CACCGAGGCC AGCAGCACCA GCCAGGCCGT GGAGGACTGC 
CTGCACGTCA GAGCCATCAA GACCGAGAGC TCCGGGCTGT GTCAGTCCAG CCCCGGGGCC 
CAGTCGTCCT GCAGCAGCGA GCCCTCTCCT CTGGGCAGTG CCCCCAACAA TGACAGTGGC 
GTGGAGATGC CGGGGACGGG GCCCGGGAGC CTGGGAGACC TGACGGCACT GGATGACACA 
CCCCCAGGGG CCGACACCTC AGCCCTGGCT GCCCCCTCCG CTGGTGGCCT CCAGCTGCGC 
AAACACATGA CCACCATGCA CCGGTTCGAG CAGCTCAAGA AGGAGAAGCT CAAGTCACTC 
AAGGATTCCT GCTCATGGGC CGGGCCGACT CCACACACGC GGAACACCAA GCTGCCTCCC 
CTCCCGGGAA GTGGCTCCAT CCTGGAAAAC TTCAGTGGCA GTGGGGGCGG CGGGCCCGCG 
GGGCTGCTGC CGAACCCGCG GCTGTCGGAG CTGTCCGCGA GCGAGGTGAC CATGCTGAGC 
CAGCTGCAGG AGCGCCGCGA CAGCTCCACC AGCACGGTCA GCTCGGCCTA CACCGTGAGC 
CGCCGCTCCT CCGGCATCTC CCCCTACTTC TCCAGCCGCC GCTCCAGCGA GGCCTCGCCC 
CTGGGCGCCG GCCGCCCGCA CAACGCGAGC TCCGCTGACT CCTACGACCC CATCTCCACG 
GACGCGTCGC GGCGCTCGAG CGAGGCCAGC CAGTGCAGCG GCGGCTCCGG GCTGCTCAAC 
CTCACGCCGG CGCAGCAGTA CAGCCTGCGG GCCAAGTACG CGGCAGCCAC TGGCGGCCCC 
CCGCCCACTC CGCTGCCGGG CCTGGAGCGC ATGAGCCTGC GGACCAGGCT GGCGCTGCTG 
GACGCGCCCG AGCGCACGCT GCCCGCCGGC TGCCCACGCC CACTGGGGCC GCGGCGTGGC 
AGCGACGGGC CGACCTATGG CCACGGCCAC GCGGGGGCTG CGCCCGCCTT CCCCCACGAG 
GCTCCAGGCG GCGGAGCCAG GCGGGCCAGC GACCCTGTGC GGCGGCCCGA TGCCCTGTCC 
CTGCCGCGGG TGCAGCGCTT CCACAGCACC CACAACGTGA ACCCCGGCCC GCTGCCGCCC 
TGTGCCGACA GGCGAGGCCT CCGCCTGCAG AGCCACCCGA GCACCGACGG CGGCCTGGCC 
CGCGGCGCCT ACTCGCCCCG GCCGCCTAGC ATCAGCGAGA ACGTGGCGAT GGAGGCCGTG 
GCGGCAGGAG TGGACGGCGC GGGGCCCGAG GCCGACCTGG GGCTGCCGGA GGACGACCTG 
GTGCTTCCAG ACGACGTGGT GCAGTACATC AAGGCGCACG CCAGTGGCGC TCTGGACGAG 
GGCACCGGGC AGGTGTATCC CACGGAAAGC ACTGGCTTCT CTGACAACCC CAGACTACCC 
AGCCCGGGGC TGCACGGCCA GCGCAGGATG GTGGCTGCGG ACTCCAACGT GGGCCCCTCC 
GCCCCTATGC TGGGAGGATG CCAGTTAGGC TTTGGGGCGC CCTCCAGCCT GAACAAAAAT 
AACATGCCTG TGCAGTGGAA TGAGGTGAGC TCCGGCACCG TAGACGCCCT GGCCAGCCAG 
GTGAAGCCTC CACCCTTTCC TCAGGGCAAC CTGGCGGTGG TGCAGCAGAA GCCTGCCTTT 
GGCCAGTACC CGGGCTACAG TCCGCAAGGC CTACAGGCTA GCCCTGGGGG CCTGGACAGC 
ACGCAGCCAC ACCTGCAGCC CCGCAGCGGA GCCCCCTCCC AGGGCATCCC CAGGGTAAAC 
TACATGCAGC AGCTGCGACA GCCAGTGGCA GGCAGCCAGT GTCCTGGCAT GACTACCACT 
ATGAGCCCCC ATGCCTGCTA TGGCCAAGTC CACCCCCAGC TGAGCCCCAG CACCATCAGT 
GGGGCCCTCA ACCAGTTCCC CCAATCCTGC AGCAACATGC CAGCCAAGCC AGGGCATCTG 
GGGCACCCTC AGCAGACAGA AGTGGCACCT GACCCCACCA CGATGGGCAA TCGCCACAGG 
GAACTTGGGG TCCCCGATTC AGCCCTGGCT GGAGTGCCAC CACCTCACCC AGTCCAGAGC 
TACCCACAGC AGAGCCATCA CCTGGCAGCC TCCATGAGCC AGGAGGGCTA CCACCAGGTC 
CCCAGCCTTC TGCCTGCCCG CCAGCCTGGC TTCATGGAGC CCCAAACAGG CCCGATGGGG 
GTGGCTACAG CAGGCTTTGG CCTAGTGCAG CCCCGGCCTC CCCTCGAGCC CAGCCCCACT 
GGCCGCCACC GTGGGGTACG TGCTGTGCAG CAGCAGCTGG CCTACGCCAG GGCCACAGGC 
CATGCCATGG CTGCCATGCC GTCCAGTCAG GAAACAGCAG AGGCTGTGCC CAAGGGAGCG 
ATGGGCAACA TGGGGTCGGT GCCTCCCCAG CCGCCTCCGC AGGACGCAGG TGGGGCCCCG 
GACCACAGCA TGCTCTACTA CTACGGCCAG ATCCACATGT ACGAACAGGA TGGAGGCCTG 
GAGAACCTCG GGAGCTGCCA GGTCATGCGG TCCCAGCCAC CACAGCCACA GGCCTGTCAG 
GACAGCATCC AGCCCCAGCC CTTGCCCTCA CCAGGGGTCA ACCAGGTGTC CAGCACTGTG 
GACTCCCAGC TCCTGGAGGC CCCCCAGATT GACTTCGATG CCATCATGGA TGATGGCGAT 
CACTCGAGTT TGTTCTCGGG TGCTCTGAGC CCCAGCCTCC TCCACAGCCT CTCCCAGAAC 
TCCTCCCGCC TCACCACCCC CCGAAACTCC TTGACCCTGC CCTCCATCCC CGCAGGCATC 
AGCAACATGG CTGTCGGGGA CATGAGCTCC ATGCTCACCA GCCTCGCCGA GGAGAGCAAG 
TTCCTGAACA TGATGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( NM_005270.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005261.2 Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28661
Clone ID Related Accession (Same CDS sequence)	NM_005270.4 , NM_001371271.1 , XM_024452795.1 , NM_005270.5 , XM_024452794.1
Accession Version	NM_005270.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	zinc finger protein GLI2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC017033.5 and AC016764.8. On or before Jul 6, 2019 this sequence version replaced XM_024452795.1, NM_005270.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: DQ086814.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG 
GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG 
GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG 
CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC 
CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG 
ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG 
TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC 
ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG 
AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG 
ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT 
GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC 
CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC 
GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC 
AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC 
CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG 
AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA 
CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG 
CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG 
GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG 
CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGGCCAGGT GTCTGGACAC 
GGCTCATGTG GGTGTGCCCT TCCCCTCTCC CAGGAGCAGC TGGCTGACCT CAAGGAAGAT 
CTGGACAGGG ATGACTGTAA GCAGGAGGCT GAGGTGGTCA TCTATGAGAC CAACTGCCAC 
TGGGAAGACT GCACCAAGGA GTACGACACC CAGGAGCAGC TGGTGCATCA CATCAACAAC 
GAGCACATCC ACGGGGAGAA GAAGGAGTTT GTGTGCCGCT GGCAGGCCTG CACGCGGGAG 
CAGAAGCCCT TCAAGGCGCA GTACATGCTG GTGGTGCACA TGCGGCGACA CACGGGCGAG 
AAGCCCCACA AGTGCACGTT CGAGGGCTGC TCGAAGGCCT ACTCCCGCCT GGAGAACCTG 
AAGACACACC TGCGGTCCCA CACCGGGGAG AAGCCATATG TGTGTGAGCA CGAGGGCTGC 
AACAAAGCCT TCTCCAACGC CTCGGACCGC GCCAAGCACC AGAATCGCAC CCACTCCAAC 
GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GATACACAGA CCCCAGCTCT 
CTCCGGAAGC ATGTGAAAAC GGTCCACGGC CCAGATGCCC ACGTCACCAA GAAGCAGCGC 
AATGACGTGC ACCTCCGCAC ACCGCTGCTC AAAGAGAATG GGGACAGTGA GGCCGGCACG 
GAGCCTGGCG GCCCAGAGAG CACCGAGGCC AGCAGCACCA GCCAGGCCGT GGAGGACTGC 
CTGCACGTCA GAGCCATCAA GACCGAGAGC TCCGGGCTGT GTCAGTCCAG CCCCGGGGCC 
CAGTCGTCCT GCAGCAGCGA GCCCTCTCCT CTGGGCAGTG CCCCCAACAA TGACAGTGGC 
GTGGAGATGC CGGGGACGGG GCCCGGGAGC CTGGGAGACC TGACGGCACT GGATGACACA 
CCCCCAGGGG CCGACACCTC AGCCCTGGCT GCCCCCTCCG CTGGTGGCCT CCAGCTGCGC 
AAACACATGA CCACCATGCA CCGGTTCGAG CAGCTCAAGA AGGAGAAGCT CAAGTCACTC 
AAGGATTCCT GCTCATGGGC CGGGCCGACT CCACACACGC GGAACACCAA GCTGCCTCCC 
CTCCCGGGAA GTGGCTCCAT CCTGGAAAAC TTCAGTGGCA GTGGGGGCGG CGGGCCCGCG 
GGGCTGCTGC CGAACCCGCG GCTGTCGGAG CTGTCCGCGA GCGAGGTGAC CATGCTGAGC 
CAGCTGCAGG AGCGCCGCGA CAGCTCCACC AGCACGGTCA GCTCGGCCTA CACCGTGAGC 
CGCCGCTCCT CCGGCATCTC CCCCTACTTC TCCAGCCGCC GCTCCAGCGA GGCCTCGCCC 
CTGGGCGCCG GCCGCCCGCA CAACGCGAGC TCCGCTGACT CCTACGACCC CATCTCCACG 
GACGCGTCGC GGCGCTCGAG CGAGGCCAGC CAGTGCAGCG GCGGCTCCGG GCTGCTCAAC 
CTCACGCCGG CGCAGCAGTA CAGCCTGCGG GCCAAGTACG CGGCAGCCAC TGGCGGCCCC 
CCGCCCACTC CGCTGCCGGG CCTGGAGCGC ATGAGCCTGC GGACCAGGCT GGCGCTGCTG 
GACGCGCCCG AGCGCACGCT GCCCGCCGGC TGCCCACGCC CACTGGGGCC GCGGCGTGGC 
AGCGACGGGC CGACCTATGG CCACGGCCAC GCGGGGGCTG CGCCCGCCTT CCCCCACGAG 
GCTCCAGGCG GCGGAGCCAG GCGGGCCAGC GACCCTGTGC GGCGGCCCGA TGCCCTGTCC 
CTGCCGCGGG TGCAGCGCTT CCACAGCACC CACAACGTGA ACCCCGGCCC GCTGCCGCCC 
TGTGCCGACA GGCGAGGCCT CCGCCTGCAG AGCCACCCGA GCACCGACGG CGGCCTGGCC 
CGCGGCGCCT ACTCGCCCCG GCCGCCTAGC ATCAGCGAGA ACGTGGCGAT GGAGGCCGTG 
GCGGCAGGAG TGGACGGCGC GGGGCCCGAG GCCGACCTGG GGCTGCCGGA GGACGACCTG 
GTGCTTCCAG ACGACGTGGT GCAGTACATC AAGGCGCACG CCAGTGGCGC TCTGGACGAG 
GGCACCGGGC AGGTGTATCC CACGGAAAGC ACTGGCTTCT CTGACAACCC CAGACTACCC 
AGCCCGGGGC TGCACGGCCA GCGCAGGATG GTGGCTGCGG ACTCCAACGT GGGCCCCTCC 
GCCCCTATGC TGGGAGGATG CCAGTTAGGC TTTGGGGCGC CCTCCAGCCT GAACAAAAAT 
AACATGCCTG TGCAGTGGAA TGAGGTGAGC TCCGGCACCG TAGACGCCCT GGCCAGCCAG 
GTGAAGCCTC CACCCTTTCC TCAGGGCAAC CTGGCGGTGG TGCAGCAGAA GCCTGCCTTT 
GGCCAGTACC CGGGCTACAG TCCGCAAGGC CTACAGGCTA GCCCTGGGGG CCTGGACAGC 
ACGCAGCCAC ACCTGCAGCC CCGCAGCGGA GCCCCCTCCC AGGGCATCCC CAGGGTAAAC 
TACATGCAGC AGCTGCGACA GCCAGTGGCA GGCAGCCAGT GTCCTGGCAT GACTACCACT 
ATGAGCCCCC ATGCCTGCTA TGGCCAAGTC CACCCCCAGC TGAGCCCCAG CACCATCAGT 
GGGGCCCTCA ACCAGTTCCC CCAATCCTGC AGCAACATGC CAGCCAAGCC AGGGCATCTG 
GGGCACCCTC AGCAGACAGA AGTGGCACCT GACCCCACCA CGATGGGCAA TCGCCACAGG 
GAACTTGGGG TCCCCGATTC AGCCCTGGCT GGAGTGCCAC CACCTCACCC AGTCCAGAGC 
TACCCACAGC AGAGCCATCA CCTGGCAGCC TCCATGAGCC AGGAGGGCTA CCACCAGGTC 
CCCAGCCTTC TGCCTGCCCG CCAGCCTGGC TTCATGGAGC CCCAAACAGG CCCGATGGGG 
GTGGCTACAG CAGGCTTTGG CCTAGTGCAG CCCCGGCCTC CCCTCGAGCC CAGCCCCACT 
GGCCGCCACC GTGGGGTACG TGCTGTGCAG CAGCAGCTGG CCTACGCCAG GGCCACAGGC 
CATGCCATGG CTGCCATGCC GTCCAGTCAG GAAACAGCAG AGGCTGTGCC CAAGGGAGCG 
ATGGGCAACA TGGGGTCGGT GCCTCCCCAG CCGCCTCCGC AGGACGCAGG TGGGGCCCCG 
GACCACAGCA TGCTCTACTA CTACGGCCAG ATCCACATGT ACGAACAGGA TGGAGGCCTG 
GAGAACCTCG GGAGCTGCCA GGTCATGCGG TCCCAGCCAC CACAGCCACA GGCCTGTCAG 
GACAGCATCC AGCCCCAGCC CTTGCCCTCA CCAGGGGTCA ACCAGGTGTC CAGCACTGTG 
GACTCCCAGC TCCTGGAGGC CCCCCAGATT GACTTCGATG CCATCATGGA TGATGGCGAT 
CACTCGAGTT TGTTCTCGGG TGCTCTGAGC CCCAGCCTCC TCCACAGCCT CTCCCAGAAC 
TCCTCCCGCC TCACCACCCC CCGAAACTCC TTGACCCTGC CCTCCATCCC CGCAGGCATC 
AGCAACATGG CTGTCGGGGA CATGAGCTCC ATGCTCACCA GCCTCGCCGA GGAGAGCAAG 
TTCCTGAACA TGATGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005261.2
CDS	189..4949
Translation	METSASATASEKQEAKSGILEAAGFPDPGKKASPLVVAAAAAAAVAAQGVPQHLLPPFHAPLPIDMRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQGQVSGHGSCGCALPLSQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	NM_005270.5
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005270.5

ORF Insert Sequence:

ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG 
GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG 
GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG 
CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC 
CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG 
ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG 
TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC 
ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG 
AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG 
ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT 
GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC 
CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC 
GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC 
AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC 
CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG 
AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA 
CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG 
CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG 
GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG 
CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGGCCAGGT GTCTGGACAC 
GGCTCATGTG GGTGTGCCCT TCCCCTCTCC CAGGAGCAGC TGGCTGACCT CAAGGAAGAT 
CTGGACAGGG ATGACTGTAA GCAGGAGGCT GAGGTGGTCA TCTATGAGAC CAACTGCCAC 
TGGGAAGACT GCACCAAGGA GTACGACACC CAGGAGCAGC TGGTGCATCA CATCAACAAC 
GAGCACATCC ACGGGGAGAA GAAGGAGTTT GTGTGCCGCT GGCAGGCCTG CACGCGGGAG 
CAGAAGCCCT TCAAGGCGCA GTACATGCTG GTGGTGCACA TGCGGCGACA CACGGGCGAG 
AAGCCCCACA AGTGCACGTT CGAGGGCTGC TCGAAGGCCT ACTCCCGCCT GGAGAACCTG 
AAGACACACC TGCGGTCCCA CACCGGGGAG AAGCCATATG TGTGTGAGCA CGAGGGCTGC 
AACAAAGCCT TCTCCAACGC CTCGGACCGC GCCAAGCACC AGAATCGCAC CCACTCCAAC 
GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GATACACAGA CCCCAGCTCT 
CTCCGGAAGC ATGTGAAAAC GGTCCACGGC CCAGATGCCC ACGTCACCAA GAAGCAGCGC 
AATGACGTGC ACCTCCGCAC ACCGCTGCTC AAAGAGAATG GGGACAGTGA GGCCGGCACG 
GAGCCTGGCG GCCCAGAGAG CACCGAGGCC AGCAGCACCA GCCAGGCCGT GGAGGACTGC 
CTGCACGTCA GAGCCATCAA GACCGAGAGC TCCGGGCTGT GTCAGTCCAG CCCCGGGGCC 
CAGTCGTCCT GCAGCAGCGA GCCCTCTCCT CTGGGCAGTG CCCCCAACAA TGACAGTGGC 
GTGGAGATGC CGGGGACGGG GCCCGGGAGC CTGGGAGACC TGACGGCACT GGATGACACA 
CCCCCAGGGG CCGACACCTC AGCCCTGGCT GCCCCCTCCG CTGGTGGCCT CCAGCTGCGC 
AAACACATGA CCACCATGCA CCGGTTCGAG CAGCTCAAGA AGGAGAAGCT CAAGTCACTC 
AAGGATTCCT GCTCATGGGC CGGGCCGACT CCACACACGC GGAACACCAA GCTGCCTCCC 
CTCCCGGGAA GTGGCTCCAT CCTGGAAAAC TTCAGTGGCA GTGGGGGCGG CGGGCCCGCG 
GGGCTGCTGC CGAACCCGCG GCTGTCGGAG CTGTCCGCGA GCGAGGTGAC CATGCTGAGC 
CAGCTGCAGG AGCGCCGCGA CAGCTCCACC AGCACGGTCA GCTCGGCCTA CACCGTGAGC 
CGCCGCTCCT CCGGCATCTC CCCCTACTTC TCCAGCCGCC GCTCCAGCGA GGCCTCGCCC 
CTGGGCGCCG GCCGCCCGCA CAACGCGAGC TCCGCTGACT CCTACGACCC CATCTCCACG 
GACGCGTCGC GGCGCTCGAG CGAGGCCAGC CAGTGCAGCG GCGGCTCCGG GCTGCTCAAC 
CTCACGCCGG CGCAGCAGTA CAGCCTGCGG GCCAAGTACG CGGCAGCCAC TGGCGGCCCC 
CCGCCCACTC CGCTGCCGGG CCTGGAGCGC ATGAGCCTGC GGACCAGGCT GGCGCTGCTG 
GACGCGCCCG AGCGCACGCT GCCCGCCGGC TGCCCACGCC CACTGGGGCC GCGGCGTGGC 
AGCGACGGGC CGACCTATGG CCACGGCCAC GCGGGGGCTG CGCCCGCCTT CCCCCACGAG 
GCTCCAGGCG GCGGAGCCAG GCGGGCCAGC GACCCTGTGC GGCGGCCCGA TGCCCTGTCC 
CTGCCGCGGG TGCAGCGCTT CCACAGCACC CACAACGTGA ACCCCGGCCC GCTGCCGCCC 
TGTGCCGACA GGCGAGGCCT CCGCCTGCAG AGCCACCCGA GCACCGACGG CGGCCTGGCC 
CGCGGCGCCT ACTCGCCCCG GCCGCCTAGC ATCAGCGAGA ACGTGGCGAT GGAGGCCGTG 
GCGGCAGGAG TGGACGGCGC GGGGCCCGAG GCCGACCTGG GGCTGCCGGA GGACGACCTG 
GTGCTTCCAG ACGACGTGGT GCAGTACATC AAGGCGCACG CCAGTGGCGC TCTGGACGAG 
GGCACCGGGC AGGTGTATCC CACGGAAAGC ACTGGCTTCT CTGACAACCC CAGACTACCC 
AGCCCGGGGC TGCACGGCCA GCGCAGGATG GTGGCTGCGG ACTCCAACGT GGGCCCCTCC 
GCCCCTATGC TGGGAGGATG CCAGTTAGGC TTTGGGGCGC CCTCCAGCCT GAACAAAAAT 
AACATGCCTG TGCAGTGGAA TGAGGTGAGC TCCGGCACCG TAGACGCCCT GGCCAGCCAG 
GTGAAGCCTC CACCCTTTCC TCAGGGCAAC CTGGCGGTGG TGCAGCAGAA GCCTGCCTTT 
GGCCAGTACC CGGGCTACAG TCCGCAAGGC CTACAGGCTA GCCCTGGGGG CCTGGACAGC 
ACGCAGCCAC ACCTGCAGCC CCGCAGCGGA GCCCCCTCCC AGGGCATCCC CAGGGTAAAC 
TACATGCAGC AGCTGCGACA GCCAGTGGCA GGCAGCCAGT GTCCTGGCAT GACTACCACT 
ATGAGCCCCC ATGCCTGCTA TGGCCAAGTC CACCCCCAGC TGAGCCCCAG CACCATCAGT 
GGGGCCCTCA ACCAGTTCCC CCAATCCTGC AGCAACATGC CAGCCAAGCC AGGGCATCTG 
GGGCACCCTC AGCAGACAGA AGTGGCACCT GACCCCACCA CGATGGGCAA TCGCCACAGG 
GAACTTGGGG TCCCCGATTC AGCCCTGGCT GGAGTGCCAC CACCTCACCC AGTCCAGAGC 
TACCCACAGC AGAGCCATCA CCTGGCAGCC TCCATGAGCC AGGAGGGCTA CCACCAGGTC 
CCCAGCCTTC TGCCTGCCCG CCAGCCTGGC TTCATGGAGC CCCAAACAGG CCCGATGGGG 
GTGGCTACAG CAGGCTTTGG CCTAGTGCAG CCCCGGCCTC CCCTCGAGCC CAGCCCCACT 
GGCCGCCACC GTGGGGTACG TGCTGTGCAG CAGCAGCTGG CCTACGCCAG GGCCACAGGC 
CATGCCATGG CTGCCATGCC GTCCAGTCAG GAAACAGCAG AGGCTGTGCC CAAGGGAGCG 
ATGGGCAACA TGGGGTCGGT GCCTCCCCAG CCGCCTCCGC AGGACGCAGG TGGGGCCCCG 
GACCACAGCA TGCTCTACTA CTACGGCCAG ATCCACATGT ACGAACAGGA TGGAGGCCTG 
GAGAACCTCG GGAGCTGCCA GGTCATGCGG TCCCAGCCAC CACAGCCACA GGCCTGTCAG 
GACAGCATCC AGCCCCAGCC CTTGCCCTCA CCAGGGGTCA ACCAGGTGTC CAGCACTGTG 
GACTCCCAGC TCCTGGAGGC CCCCCAGATT GACTTCGATG CCATCATGGA TGATGGCGAT 
CACTCGAGTT TGTTCTCGGG TGCTCTGAGC CCCAGCCTCC TCCACAGCCT CTCCCAGAAC 
TCCTCCCGCC TCACCACCCC CCGAAACTCC TTGACCCTGC CCTCCATCCC CGCAGGCATC 
AGCAACATGG CTGTCGGGGA CATGAGCTCC ATGCTCACCA GCCTCGCCGA GGAGAGCAAG 
TTCCTGAACA TGATGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( XM_024452795.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308563.1 Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X4, mRNA.

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CloneID	OHu28661
Clone ID Related Accession (Same CDS sequence)	NM_005270.4 , NM_001371271.1 , XM_024452795.1 , NM_005270.5 , XM_024452794.1
Accession Version	XM_024452795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	zinc finger protein GLI2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG 
GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG 
GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG 
CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC 
CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG 
ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG 
TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC 
ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG 
AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG 
ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT 
GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC 
CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC 
GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC 
AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC 
CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG 
AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA 
CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG 
CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG 
GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG 
CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGGCCAGGT GTCTGGACAC 
GGCTCATGTG GGTGTGCCCT TCCCCTCTCC CAGGAGCAGC TGGCTGACCT CAAGGAAGAT 
CTGGACAGGG ATGACTGTAA GCAGGAGGCT GAGGTGGTCA TCTATGAGAC CAACTGCCAC 
TGGGAAGACT GCACCAAGGA GTACGACACC CAGGAGCAGC TGGTGCATCA CATCAACAAC 
GAGCACATCC ACGGGGAGAA GAAGGAGTTT GTGTGCCGCT GGCAGGCCTG CACGCGGGAG 
CAGAAGCCCT TCAAGGCGCA GTACATGCTG GTGGTGCACA TGCGGCGACA CACGGGCGAG 
AAGCCCCACA AGTGCACGTT CGAGGGCTGC TCGAAGGCCT ACTCCCGCCT GGAGAACCTG 
AAGACACACC TGCGGTCCCA CACCGGGGAG AAGCCATATG TGTGTGAGCA CGAGGGCTGC 
AACAAAGCCT TCTCCAACGC CTCGGACCGC GCCAAGCACC AGAATCGCAC CCACTCCAAC 
GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GATACACAGA CCCCAGCTCT 
CTCCGGAAGC ATGTGAAAAC GGTCCACGGC CCAGATGCCC ACGTCACCAA GAAGCAGCGC 
AATGACGTGC ACCTCCGCAC ACCGCTGCTC AAAGAGAATG GGGACAGTGA GGCCGGCACG 
GAGCCTGGCG GCCCAGAGAG CACCGAGGCC AGCAGCACCA GCCAGGCCGT GGAGGACTGC 
CTGCACGTCA GAGCCATCAA GACCGAGAGC TCCGGGCTGT GTCAGTCCAG CCCCGGGGCC 
CAGTCGTCCT GCAGCAGCGA GCCCTCTCCT CTGGGCAGTG CCCCCAACAA TGACAGTGGC 
GTGGAGATGC CGGGGACGGG GCCCGGGAGC CTGGGAGACC TGACGGCACT GGATGACACA 
CCCCCAGGGG CCGACACCTC AGCCCTGGCT GCCCCCTCCG CTGGTGGCCT CCAGCTGCGC 
AAACACATGA CCACCATGCA CCGGTTCGAG CAGCTCAAGA AGGAGAAGCT CAAGTCACTC 
AAGGATTCCT GCTCATGGGC CGGGCCGACT CCACACACGC GGAACACCAA GCTGCCTCCC 
CTCCCGGGAA GTGGCTCCAT CCTGGAAAAC TTCAGTGGCA GTGGGGGCGG CGGGCCCGCG 
GGGCTGCTGC CGAACCCGCG GCTGTCGGAG CTGTCCGCGA GCGAGGTGAC CATGCTGAGC 
CAGCTGCAGG AGCGCCGCGA CAGCTCCACC AGCACGGTCA GCTCGGCCTA CACCGTGAGC 
CGCCGCTCCT CCGGCATCTC CCCCTACTTC TCCAGCCGCC GCTCCAGCGA GGCCTCGCCC 
CTGGGCGCCG GCCGCCCGCA CAACGCGAGC TCCGCTGACT CCTACGACCC CATCTCCACG 
GACGCGTCGC GGCGCTCGAG CGAGGCCAGC CAGTGCAGCG GCGGCTCCGG GCTGCTCAAC 
CTCACGCCGG CGCAGCAGTA CAGCCTGCGG GCCAAGTACG CGGCAGCCAC TGGCGGCCCC 
CCGCCCACTC CGCTGCCGGG CCTGGAGCGC ATGAGCCTGC GGACCAGGCT GGCGCTGCTG 
GACGCGCCCG AGCGCACGCT GCCCGCCGGC TGCCCACGCC CACTGGGGCC GCGGCGTGGC 
AGCGACGGGC CGACCTATGG CCACGGCCAC GCGGGGGCTG CGCCCGCCTT CCCCCACGAG 
GCTCCAGGCG GCGGAGCCAG GCGGGCCAGC GACCCTGTGC GGCGGCCCGA TGCCCTGTCC 
CTGCCGCGGG TGCAGCGCTT CCACAGCACC CACAACGTGA ACCCCGGCCC GCTGCCGCCC 
TGTGCCGACA GGCGAGGCCT CCGCCTGCAG AGCCACCCGA GCACCGACGG CGGCCTGGCC 
CGCGGCGCCT ACTCGCCCCG GCCGCCTAGC ATCAGCGAGA ACGTGGCGAT GGAGGCCGTG 
GCGGCAGGAG TGGACGGCGC GGGGCCCGAG GCCGACCTGG GGCTGCCGGA GGACGACCTG 
GTGCTTCCAG ACGACGTGGT GCAGTACATC AAGGCGCACG CCAGTGGCGC TCTGGACGAG 
GGCACCGGGC AGGTGTATCC CACGGAAAGC ACTGGCTTCT CTGACAACCC CAGACTACCC 
AGCCCGGGGC TGCACGGCCA GCGCAGGATG GTGGCTGCGG ACTCCAACGT GGGCCCCTCC 
GCCCCTATGC TGGGAGGATG CCAGTTAGGC TTTGGGGCGC CCTCCAGCCT GAACAAAAAT 
AACATGCCTG TGCAGTGGAA TGAGGTGAGC TCCGGCACCG TAGACGCCCT GGCCAGCCAG 
GTGAAGCCTC CACCCTTTCC TCAGGGCAAC CTGGCGGTGG TGCAGCAGAA GCCTGCCTTT 
GGCCAGTACC CGGGCTACAG TCCGCAAGGC CTACAGGCTA GCCCTGGGGG CCTGGACAGC 
ACGCAGCCAC ACCTGCAGCC CCGCAGCGGA GCCCCCTCCC AGGGCATCCC CAGGGTAAAC 
TACATGCAGC AGCTGCGACA GCCAGTGGCA GGCAGCCAGT GTCCTGGCAT GACTACCACT 
ATGAGCCCCC ATGCCTGCTA TGGCCAAGTC CACCCCCAGC TGAGCCCCAG CACCATCAGT 
GGGGCCCTCA ACCAGTTCCC CCAATCCTGC AGCAACATGC CAGCCAAGCC AGGGCATCTG 
GGGCACCCTC AGCAGACAGA AGTGGCACCT GACCCCACCA CGATGGGCAA TCGCCACAGG 
GAACTTGGGG TCCCCGATTC AGCCCTGGCT GGAGTGCCAC CACCTCACCC AGTCCAGAGC 
TACCCACAGC AGAGCCATCA CCTGGCAGCC TCCATGAGCC AGGAGGGCTA CCACCAGGTC 
CCCAGCCTTC TGCCTGCCCG CCAGCCTGGC TTCATGGAGC CCCAAACAGG CCCGATGGGG 
GTGGCTACAG CAGGCTTTGG CCTAGTGCAG CCCCGGCCTC CCCTCGAGCC CAGCCCCACT 
GGCCGCCACC GTGGGGTACG TGCTGTGCAG CAGCAGCTGG CCTACGCCAG GGCCACAGGC 
CATGCCATGG CTGCCATGCC GTCCAGTCAG GAAACAGCAG AGGCTGTGCC CAAGGGAGCG 
ATGGGCAACA TGGGGTCGGT GCCTCCCCAG CCGCCTCCGC AGGACGCAGG TGGGGCCCCG 
GACCACAGCA TGCTCTACTA CTACGGCCAG ATCCACATGT ACGAACAGGA TGGAGGCCTG 
GAGAACCTCG GGAGCTGCCA GGTCATGCGG TCCCAGCCAC CACAGCCACA GGCCTGTCAG 
GACAGCATCC AGCCCCAGCC CTTGCCCTCA CCAGGGGTCA ACCAGGTGTC CAGCACTGTG 
GACTCCCAGC TCCTGGAGGC CCCCCAGATT GACTTCGATG CCATCATGGA TGATGGCGAT 
CACTCGAGTT TGTTCTCGGG TGCTCTGAGC CCCAGCCTCC TCCACAGCCT CTCCCAGAAC 
TCCTCCCGCC TCACCACCCC CCGAAACTCC TTGACCCTGC CCTCCATCCC CGCAGGCATC 
AGCAACATGG CTGTCGGGGA CATGAGCTCC ATGCTCACCA GCCTCGCCGA GGAGAGCAAG 
TTCCTGAACA TGATGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308563.1
CDS	3202..7962
Translation	METSASATASEKQEAKSGILEAAGFPDPGKKASPLVVAAAAAAAVAAQGVPQHLLPPFHAPLPIDMRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQGQVSGHGSCGCALPLSQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	XM_024452795.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452795.1

ORF Insert Sequence:

ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG 
GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG 
GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG 
CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC 
CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG 
ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG 
TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC 
ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG 
AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG 
ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT 
GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC 
CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC 
GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC 
AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC 
CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG 
AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA 
CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG 
CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG 
GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG 
CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGGCCAGGT GTCTGGACAC 
GGCTCATGTG GGTGTGCCCT TCCCCTCTCC CAGGAGCAGC TGGCTGACCT CAAGGAAGAT 
CTGGACAGGG ATGACTGTAA GCAGGAGGCT GAGGTGGTCA TCTATGAGAC CAACTGCCAC 
TGGGAAGACT GCACCAAGGA GTACGACACC CAGGAGCAGC TGGTGCATCA CATCAACAAC 
GAGCACATCC ACGGGGAGAA GAAGGAGTTT GTGTGCCGCT GGCAGGCCTG CACGCGGGAG 
CAGAAGCCCT TCAAGGCGCA GTACATGCTG GTGGTGCACA TGCGGCGACA CACGGGCGAG 
AAGCCCCACA AGTGCACGTT CGAGGGCTGC TCGAAGGCCT ACTCCCGCCT GGAGAACCTG 
AAGACACACC TGCGGTCCCA CACCGGGGAG AAGCCATATG TGTGTGAGCA CGAGGGCTGC 
AACAAAGCCT TCTCCAACGC CTCGGACCGC GCCAAGCACC AGAATCGCAC CCACTCCAAC 
GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GATACACAGA CCCCAGCTCT 
CTCCGGAAGC ATGTGAAAAC GGTCCACGGC CCAGATGCCC ACGTCACCAA GAAGCAGCGC 
AATGACGTGC ACCTCCGCAC ACCGCTGCTC AAAGAGAATG GGGACAGTGA GGCCGGCACG 
GAGCCTGGCG GCCCAGAGAG CACCGAGGCC AGCAGCACCA GCCAGGCCGT GGAGGACTGC 
CTGCACGTCA GAGCCATCAA GACCGAGAGC TCCGGGCTGT GTCAGTCCAG CCCCGGGGCC 
CAGTCGTCCT GCAGCAGCGA GCCCTCTCCT CTGGGCAGTG CCCCCAACAA TGACAGTGGC 
GTGGAGATGC CGGGGACGGG GCCCGGGAGC CTGGGAGACC TGACGGCACT GGATGACACA 
CCCCCAGGGG CCGACACCTC AGCCCTGGCT GCCCCCTCCG CTGGTGGCCT CCAGCTGCGC 
AAACACATGA CCACCATGCA CCGGTTCGAG CAGCTCAAGA AGGAGAAGCT CAAGTCACTC 
AAGGATTCCT GCTCATGGGC CGGGCCGACT CCACACACGC GGAACACCAA GCTGCCTCCC 
CTCCCGGGAA GTGGCTCCAT CCTGGAAAAC TTCAGTGGCA GTGGGGGCGG CGGGCCCGCG 
GGGCTGCTGC CGAACCCGCG GCTGTCGGAG CTGTCCGCGA GCGAGGTGAC CATGCTGAGC 
CAGCTGCAGG AGCGCCGCGA CAGCTCCACC AGCACGGTCA GCTCGGCCTA CACCGTGAGC 
CGCCGCTCCT CCGGCATCTC CCCCTACTTC TCCAGCCGCC GCTCCAGCGA GGCCTCGCCC 
CTGGGCGCCG GCCGCCCGCA CAACGCGAGC TCCGCTGACT CCTACGACCC CATCTCCACG 
GACGCGTCGC GGCGCTCGAG CGAGGCCAGC CAGTGCAGCG GCGGCTCCGG GCTGCTCAAC 
CTCACGCCGG CGCAGCAGTA CAGCCTGCGG GCCAAGTACG CGGCAGCCAC TGGCGGCCCC 
CCGCCCACTC CGCTGCCGGG CCTGGAGCGC ATGAGCCTGC GGACCAGGCT GGCGCTGCTG 
GACGCGCCCG AGCGCACGCT GCCCGCCGGC TGCCCACGCC CACTGGGGCC GCGGCGTGGC 
AGCGACGGGC CGACCTATGG CCACGGCCAC GCGGGGGCTG CGCCCGCCTT CCCCCACGAG 
GCTCCAGGCG GCGGAGCCAG GCGGGCCAGC GACCCTGTGC GGCGGCCCGA TGCCCTGTCC 
CTGCCGCGGG TGCAGCGCTT CCACAGCACC CACAACGTGA ACCCCGGCCC GCTGCCGCCC 
TGTGCCGACA GGCGAGGCCT CCGCCTGCAG AGCCACCCGA GCACCGACGG CGGCCTGGCC 
CGCGGCGCCT ACTCGCCCCG GCCGCCTAGC ATCAGCGAGA ACGTGGCGAT GGAGGCCGTG 
GCGGCAGGAG TGGACGGCGC GGGGCCCGAG GCCGACCTGG GGCTGCCGGA GGACGACCTG 
GTGCTTCCAG ACGACGTGGT GCAGTACATC AAGGCGCACG CCAGTGGCGC TCTGGACGAG 
GGCACCGGGC AGGTGTATCC CACGGAAAGC ACTGGCTTCT CTGACAACCC CAGACTACCC 
AGCCCGGGGC TGCACGGCCA GCGCAGGATG GTGGCTGCGG ACTCCAACGT GGGCCCCTCC 
GCCCCTATGC TGGGAGGATG CCAGTTAGGC TTTGGGGCGC CCTCCAGCCT GAACAAAAAT 
AACATGCCTG TGCAGTGGAA TGAGGTGAGC TCCGGCACCG TAGACGCCCT GGCCAGCCAG 
GTGAAGCCTC CACCCTTTCC TCAGGGCAAC CTGGCGGTGG TGCAGCAGAA GCCTGCCTTT 
GGCCAGTACC CGGGCTACAG TCCGCAAGGC CTACAGGCTA GCCCTGGGGG CCTGGACAGC 
ACGCAGCCAC ACCTGCAGCC CCGCAGCGGA GCCCCCTCCC AGGGCATCCC CAGGGTAAAC 
TACATGCAGC AGCTGCGACA GCCAGTGGCA GGCAGCCAGT GTCCTGGCAT GACTACCACT 
ATGAGCCCCC ATGCCTGCTA TGGCCAAGTC CACCCCCAGC TGAGCCCCAG CACCATCAGT 
GGGGCCCTCA ACCAGTTCCC CCAATCCTGC AGCAACATGC CAGCCAAGCC AGGGCATCTG 
GGGCACCCTC AGCAGACAGA AGTGGCACCT GACCCCACCA CGATGGGCAA TCGCCACAGG 
GAACTTGGGG TCCCCGATTC AGCCCTGGCT GGAGTGCCAC CACCTCACCC AGTCCAGAGC 
TACCCACAGC AGAGCCATCA CCTGGCAGCC TCCATGAGCC AGGAGGGCTA CCACCAGGTC 
CCCAGCCTTC TGCCTGCCCG CCAGCCTGGC TTCATGGAGC CCCAAACAGG CCCGATGGGG 
GTGGCTACAG CAGGCTTTGG CCTAGTGCAG CCCCGGCCTC CCCTCGAGCC CAGCCCCACT 
GGCCGCCACC GTGGGGTACG TGCTGTGCAG CAGCAGCTGG CCTACGCCAG GGCCACAGGC 
CATGCCATGG CTGCCATGCC GTCCAGTCAG GAAACAGCAG AGGCTGTGCC CAAGGGAGCG 
ATGGGCAACA TGGGGTCGGT GCCTCCCCAG CCGCCTCCGC AGGACGCAGG TGGGGCCCCG 
GACCACAGCA TGCTCTACTA CTACGGCCAG ATCCACATGT ACGAACAGGA TGGAGGCCTG 
GAGAACCTCG GGAGCTGCCA GGTCATGCGG TCCCAGCCAC CACAGCCACA GGCCTGTCAG 
GACAGCATCC AGCCCCAGCC CTTGCCCTCA CCAGGGGTCA ACCAGGTGTC CAGCACTGTG 
GACTCCCAGC TCCTGGAGGC CCCCCAGATT GACTTCGATG CCATCATGGA TGATGGCGAT 
CACTCGAGTT TGTTCTCGGG TGCTCTGAGC CCCAGCCTCC TCCACAGCCT CTCCCAGAAC 
TCCTCCCGCC TCACCACCCC CCGAAACTCC TTGACCCTGC CCTCCATCCC CGCAGGCATC 
AGCAACATGG CTGTCGGGGA CATGAGCTCC ATGCTCACCA GCCTCGCCGA GGAGAGCAAG 
TTCCTGAACA TGATGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( XM_024452794.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308562.1 Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X3, mRNA.

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CloneID	OHu28661
Clone ID Related Accession (Same CDS sequence)	NM_005270.4 , NM_001371271.1 , XM_024452795.1 , NM_005270.5 , XM_024452794.1
Accession Version	XM_024452794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	zinc finger protein GLI2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG 
GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG 
GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG 
CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC 
CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG 
ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG 
TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC 
ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG 
AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG 
ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT 
GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC 
CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC 
GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC 
AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC 
CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG 
AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA 
CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG 
CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG 
GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG 
CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGGCCAGGT GTCTGGACAC 
GGCTCATGTG GGTGTGCCCT TCCCCTCTCC CAGGAGCAGC TGGCTGACCT CAAGGAAGAT 
CTGGACAGGG ATGACTGTAA GCAGGAGGCT GAGGTGGTCA TCTATGAGAC CAACTGCCAC 
TGGGAAGACT GCACCAAGGA GTACGACACC CAGGAGCAGC TGGTGCATCA CATCAACAAC 
GAGCACATCC ACGGGGAGAA GAAGGAGTTT GTGTGCCGCT GGCAGGCCTG CACGCGGGAG 
CAGAAGCCCT TCAAGGCGCA GTACATGCTG GTGGTGCACA TGCGGCGACA CACGGGCGAG 
AAGCCCCACA AGTGCACGTT CGAGGGCTGC TCGAAGGCCT ACTCCCGCCT GGAGAACCTG 
AAGACACACC TGCGGTCCCA CACCGGGGAG AAGCCATATG TGTGTGAGCA CGAGGGCTGC 
AACAAAGCCT TCTCCAACGC CTCGGACCGC GCCAAGCACC AGAATCGCAC CCACTCCAAC 
GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GATACACAGA CCCCAGCTCT 
CTCCGGAAGC ATGTGAAAAC GGTCCACGGC CCAGATGCCC ACGTCACCAA GAAGCAGCGC 
AATGACGTGC ACCTCCGCAC ACCGCTGCTC AAAGAGAATG GGGACAGTGA GGCCGGCACG 
GAGCCTGGCG GCCCAGAGAG CACCGAGGCC AGCAGCACCA GCCAGGCCGT GGAGGACTGC 
CTGCACGTCA GAGCCATCAA GACCGAGAGC TCCGGGCTGT GTCAGTCCAG CCCCGGGGCC 
CAGTCGTCCT GCAGCAGCGA GCCCTCTCCT CTGGGCAGTG CCCCCAACAA TGACAGTGGC 
GTGGAGATGC CGGGGACGGG GCCCGGGAGC CTGGGAGACC TGACGGCACT GGATGACACA 
CCCCCAGGGG CCGACACCTC AGCCCTGGCT GCCCCCTCCG CTGGTGGCCT CCAGCTGCGC 
AAACACATGA CCACCATGCA CCGGTTCGAG CAGCTCAAGA AGGAGAAGCT CAAGTCACTC 
AAGGATTCCT GCTCATGGGC CGGGCCGACT CCACACACGC GGAACACCAA GCTGCCTCCC 
CTCCCGGGAA GTGGCTCCAT CCTGGAAAAC TTCAGTGGCA GTGGGGGCGG CGGGCCCGCG 
GGGCTGCTGC CGAACCCGCG GCTGTCGGAG CTGTCCGCGA GCGAGGTGAC CATGCTGAGC 
CAGCTGCAGG AGCGCCGCGA CAGCTCCACC AGCACGGTCA GCTCGGCCTA CACCGTGAGC 
CGCCGCTCCT CCGGCATCTC CCCCTACTTC TCCAGCCGCC GCTCCAGCGA GGCCTCGCCC 
CTGGGCGCCG GCCGCCCGCA CAACGCGAGC TCCGCTGACT CCTACGACCC CATCTCCACG 
GACGCGTCGC GGCGCTCGAG CGAGGCCAGC CAGTGCAGCG GCGGCTCCGG GCTGCTCAAC 
CTCACGCCGG CGCAGCAGTA CAGCCTGCGG GCCAAGTACG CGGCAGCCAC TGGCGGCCCC 
CCGCCCACTC CGCTGCCGGG CCTGGAGCGC ATGAGCCTGC GGACCAGGCT GGCGCTGCTG 
GACGCGCCCG AGCGCACGCT GCCCGCCGGC TGCCCACGCC CACTGGGGCC GCGGCGTGGC 
AGCGACGGGC CGACCTATGG CCACGGCCAC GCGGGGGCTG CGCCCGCCTT CCCCCACGAG 
GCTCCAGGCG GCGGAGCCAG GCGGGCCAGC GACCCTGTGC GGCGGCCCGA TGCCCTGTCC 
CTGCCGCGGG TGCAGCGCTT CCACAGCACC CACAACGTGA ACCCCGGCCC GCTGCCGCCC 
TGTGCCGACA GGCGAGGCCT CCGCCTGCAG AGCCACCCGA GCACCGACGG CGGCCTGGCC 
CGCGGCGCCT ACTCGCCCCG GCCGCCTAGC ATCAGCGAGA ACGTGGCGAT GGAGGCCGTG 
GCGGCAGGAG TGGACGGCGC GGGGCCCGAG GCCGACCTGG GGCTGCCGGA GGACGACCTG 
GTGCTTCCAG ACGACGTGGT GCAGTACATC AAGGCGCACG CCAGTGGCGC TCTGGACGAG 
GGCACCGGGC AGGTGTATCC CACGGAAAGC ACTGGCTTCT CTGACAACCC CAGACTACCC 
AGCCCGGGGC TGCACGGCCA GCGCAGGATG GTGGCTGCGG ACTCCAACGT GGGCCCCTCC 
GCCCCTATGC TGGGAGGATG CCAGTTAGGC TTTGGGGCGC CCTCCAGCCT GAACAAAAAT 
AACATGCCTG TGCAGTGGAA TGAGGTGAGC TCCGGCACCG TAGACGCCCT GGCCAGCCAG 
GTGAAGCCTC CACCCTTTCC TCAGGGCAAC CTGGCGGTGG TGCAGCAGAA GCCTGCCTTT 
GGCCAGTACC CGGGCTACAG TCCGCAAGGC CTACAGGCTA GCCCTGGGGG CCTGGACAGC 
ACGCAGCCAC ACCTGCAGCC CCGCAGCGGA GCCCCCTCCC AGGGCATCCC CAGGGTAAAC 
TACATGCAGC AGCTGCGACA GCCAGTGGCA GGCAGCCAGT GTCCTGGCAT GACTACCACT 
ATGAGCCCCC ATGCCTGCTA TGGCCAAGTC CACCCCCAGC TGAGCCCCAG CACCATCAGT 
GGGGCCCTCA ACCAGTTCCC CCAATCCTGC AGCAACATGC CAGCCAAGCC AGGGCATCTG 
GGGCACCCTC AGCAGACAGA AGTGGCACCT GACCCCACCA CGATGGGCAA TCGCCACAGG 
GAACTTGGGG TCCCCGATTC AGCCCTGGCT GGAGTGCCAC CACCTCACCC AGTCCAGAGC 
TACCCACAGC AGAGCCATCA CCTGGCAGCC TCCATGAGCC AGGAGGGCTA CCACCAGGTC 
CCCAGCCTTC TGCCTGCCCG CCAGCCTGGC TTCATGGAGC CCCAAACAGG CCCGATGGGG 
GTGGCTACAG CAGGCTTTGG CCTAGTGCAG CCCCGGCCTC CCCTCGAGCC CAGCCCCACT 
GGCCGCCACC GTGGGGTACG TGCTGTGCAG CAGCAGCTGG CCTACGCCAG GGCCACAGGC 
CATGCCATGG CTGCCATGCC GTCCAGTCAG GAAACAGCAG AGGCTGTGCC CAAGGGAGCG 
ATGGGCAACA TGGGGTCGGT GCCTCCCCAG CCGCCTCCGC AGGACGCAGG TGGGGCCCCG 
GACCACAGCA TGCTCTACTA CTACGGCCAG ATCCACATGT ACGAACAGGA TGGAGGCCTG 
GAGAACCTCG GGAGCTGCCA GGTCATGCGG TCCCAGCCAC CACAGCCACA GGCCTGTCAG 
GACAGCATCC AGCCCCAGCC CTTGCCCTCA CCAGGGGTCA ACCAGGTGTC CAGCACTGTG 
GACTCCCAGC TCCTGGAGGC CCCCCAGATT GACTTCGATG CCATCATGGA TGATGGCGAT 
CACTCGAGTT TGTTCTCGGG TGCTCTGAGC CCCAGCCTCC TCCACAGCCT CTCCCAGAAC 
TCCTCCCGCC TCACCACCCC CCGAAACTCC TTGACCCTGC CCTCCATCCC CGCAGGCATC 
AGCAACATGG CTGTCGGGGA CATGAGCTCC ATGCTCACCA GCCTCGCCGA GGAGAGCAAG 
TTCCTGAACA TGATGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308562.1
CDS	452..5212
Translation	METSASATASEKQEAKSGILEAAGFPDPGKKASPLVVAAAAAAAVAAQGVPQHLLPPFHAPLPIDMRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQGQVSGHGSCGCALPLSQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	XM_024452794.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452794.1

ORF Insert Sequence:

ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG 
GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG 
GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG 
CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC 
CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG 
ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG 
TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC 
ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG 
AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG 
ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT 
GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC 
CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC 
GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC 
AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC 
CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG 
AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA 
CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG 
CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG 
GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG 
CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGGCCAGGT GTCTGGACAC 
GGCTCATGTG GGTGTGCCCT TCCCCTCTCC CAGGAGCAGC TGGCTGACCT CAAGGAAGAT 
CTGGACAGGG ATGACTGTAA GCAGGAGGCT GAGGTGGTCA TCTATGAGAC CAACTGCCAC 
TGGGAAGACT GCACCAAGGA GTACGACACC CAGGAGCAGC TGGTGCATCA CATCAACAAC 
GAGCACATCC ACGGGGAGAA GAAGGAGTTT GTGTGCCGCT GGCAGGCCTG CACGCGGGAG 
CAGAAGCCCT TCAAGGCGCA GTACATGCTG GTGGTGCACA TGCGGCGACA CACGGGCGAG 
AAGCCCCACA AGTGCACGTT CGAGGGCTGC TCGAAGGCCT ACTCCCGCCT GGAGAACCTG 
AAGACACACC TGCGGTCCCA CACCGGGGAG AAGCCATATG TGTGTGAGCA CGAGGGCTGC 
AACAAAGCCT TCTCCAACGC CTCGGACCGC GCCAAGCACC AGAATCGCAC CCACTCCAAC 
GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GATACACAGA CCCCAGCTCT 
CTCCGGAAGC ATGTGAAAAC GGTCCACGGC CCAGATGCCC ACGTCACCAA GAAGCAGCGC 
AATGACGTGC ACCTCCGCAC ACCGCTGCTC AAAGAGAATG GGGACAGTGA GGCCGGCACG 
GAGCCTGGCG GCCCAGAGAG CACCGAGGCC AGCAGCACCA GCCAGGCCGT GGAGGACTGC 
CTGCACGTCA GAGCCATCAA GACCGAGAGC TCCGGGCTGT GTCAGTCCAG CCCCGGGGCC 
CAGTCGTCCT GCAGCAGCGA GCCCTCTCCT CTGGGCAGTG CCCCCAACAA TGACAGTGGC 
GTGGAGATGC CGGGGACGGG GCCCGGGAGC CTGGGAGACC TGACGGCACT GGATGACACA 
CCCCCAGGGG CCGACACCTC AGCCCTGGCT GCCCCCTCCG CTGGTGGCCT CCAGCTGCGC 
AAACACATGA CCACCATGCA CCGGTTCGAG CAGCTCAAGA AGGAGAAGCT CAAGTCACTC 
AAGGATTCCT GCTCATGGGC CGGGCCGACT CCACACACGC GGAACACCAA GCTGCCTCCC 
CTCCCGGGAA GTGGCTCCAT CCTGGAAAAC TTCAGTGGCA GTGGGGGCGG CGGGCCCGCG 
GGGCTGCTGC CGAACCCGCG GCTGTCGGAG CTGTCCGCGA GCGAGGTGAC CATGCTGAGC 
CAGCTGCAGG AGCGCCGCGA CAGCTCCACC AGCACGGTCA GCTCGGCCTA CACCGTGAGC 
CGCCGCTCCT CCGGCATCTC CCCCTACTTC TCCAGCCGCC GCTCCAGCGA GGCCTCGCCC 
CTGGGCGCCG GCCGCCCGCA CAACGCGAGC TCCGCTGACT CCTACGACCC CATCTCCACG 
GACGCGTCGC GGCGCTCGAG CGAGGCCAGC CAGTGCAGCG GCGGCTCCGG GCTGCTCAAC 
CTCACGCCGG CGCAGCAGTA CAGCCTGCGG GCCAAGTACG CGGCAGCCAC TGGCGGCCCC 
CCGCCCACTC CGCTGCCGGG CCTGGAGCGC ATGAGCCTGC GGACCAGGCT GGCGCTGCTG 
GACGCGCCCG AGCGCACGCT GCCCGCCGGC TGCCCACGCC CACTGGGGCC GCGGCGTGGC 
AGCGACGGGC CGACCTATGG CCACGGCCAC GCGGGGGCTG CGCCCGCCTT CCCCCACGAG 
GCTCCAGGCG GCGGAGCCAG GCGGGCCAGC GACCCTGTGC GGCGGCCCGA TGCCCTGTCC 
CTGCCGCGGG TGCAGCGCTT CCACAGCACC CACAACGTGA ACCCCGGCCC GCTGCCGCCC 
TGTGCCGACA GGCGAGGCCT CCGCCTGCAG AGCCACCCGA GCACCGACGG CGGCCTGGCC 
CGCGGCGCCT ACTCGCCCCG GCCGCCTAGC ATCAGCGAGA ACGTGGCGAT GGAGGCCGTG 
GCGGCAGGAG TGGACGGCGC GGGGCCCGAG GCCGACCTGG GGCTGCCGGA GGACGACCTG 
GTGCTTCCAG ACGACGTGGT GCAGTACATC AAGGCGCACG CCAGTGGCGC TCTGGACGAG 
GGCACCGGGC AGGTGTATCC CACGGAAAGC ACTGGCTTCT CTGACAACCC CAGACTACCC 
AGCCCGGGGC TGCACGGCCA GCGCAGGATG GTGGCTGCGG ACTCCAACGT GGGCCCCTCC 
GCCCCTATGC TGGGAGGATG CCAGTTAGGC TTTGGGGCGC CCTCCAGCCT GAACAAAAAT 
AACATGCCTG TGCAGTGGAA TGAGGTGAGC TCCGGCACCG TAGACGCCCT GGCCAGCCAG 
GTGAAGCCTC CACCCTTTCC TCAGGGCAAC CTGGCGGTGG TGCAGCAGAA GCCTGCCTTT 
GGCCAGTACC CGGGCTACAG TCCGCAAGGC CTACAGGCTA GCCCTGGGGG CCTGGACAGC 
ACGCAGCCAC ACCTGCAGCC CCGCAGCGGA GCCCCCTCCC AGGGCATCCC CAGGGTAAAC 
TACATGCAGC AGCTGCGACA GCCAGTGGCA GGCAGCCAGT GTCCTGGCAT GACTACCACT 
ATGAGCCCCC ATGCCTGCTA TGGCCAAGTC CACCCCCAGC TGAGCCCCAG CACCATCAGT 
GGGGCCCTCA ACCAGTTCCC CCAATCCTGC AGCAACATGC CAGCCAAGCC AGGGCATCTG 
GGGCACCCTC AGCAGACAGA AGTGGCACCT GACCCCACCA CGATGGGCAA TCGCCACAGG 
GAACTTGGGG TCCCCGATTC AGCCCTGGCT GGAGTGCCAC CACCTCACCC AGTCCAGAGC 
TACCCACAGC AGAGCCATCA CCTGGCAGCC TCCATGAGCC AGGAGGGCTA CCACCAGGTC 
CCCAGCCTTC TGCCTGCCCG CCAGCCTGGC TTCATGGAGC CCCAAACAGG CCCGATGGGG 
GTGGCTACAG CAGGCTTTGG CCTAGTGCAG CCCCGGCCTC CCCTCGAGCC CAGCCCCACT 
GGCCGCCACC GTGGGGTACG TGCTGTGCAG CAGCAGCTGG CCTACGCCAG GGCCACAGGC 
CATGCCATGG CTGCCATGCC GTCCAGTCAG GAAACAGCAG AGGCTGTGCC CAAGGGAGCG 
ATGGGCAACA TGGGGTCGGT GCCTCCCCAG CCGCCTCCGC AGGACGCAGG TGGGGCCCCG 
GACCACAGCA TGCTCTACTA CTACGGCCAG ATCCACATGT ACGAACAGGA TGGAGGCCTG 
GAGAACCTCG GGAGCTGCCA GGTCATGCGG TCCCAGCCAC CACAGCCACA GGCCTGTCAG 
GACAGCATCC AGCCCCAGCC CTTGCCCTCA CCAGGGGTCA ACCAGGTGTC CAGCACTGTG 
GACTCCCAGC TCCTGGAGGC CCCCCAGATT GACTTCGATG CCATCATGGA TGATGGCGAT 
CACTCGAGTT TGTTCTCGGG TGCTCTGAGC CCCAGCCTCC TCCACAGCCT CTCCCAGAAC 
TCCTCCCGCC TCACCACCCC CCGAAACTCC TTGACCCTGC CCTCCATCCC CGCAGGCATC 
AGCAACATGG CTGTCGGGGA CATGAGCTCC ATGCTCACCA GCCTCGCCGA GGAGAGCAAG 
TTCCTGAACA TGATGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( NM_001374354.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361283.1 Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu57986
Clone ID Related Accession (Same CDS sequence)	XM_011510974.2 , NM_001374354.1
Accession Version	NM_001374354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	zinc finger protein GLI2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC017033.5 and AC016764.8. On Sep 23, 2019 this sequence version replaced XM_011510974.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DRR138518.71525.1 [ECO:0000332] ##Evidence-Data-END##

ATGGAGCACT ACCTCCGTTC TGTGCACAGC AGCCCCACGC TCTCCATGAT CTCTGCAGCC 
AGGGGCCTCA GCCCCGCTGA TGTGGCCCAG GAGCACCTTA AGGAGAGGGG ACTGTTTGGC 
CTTCCTGCTC CAGGCACCAC CCCCTCAGAC TATTACCACC AGATGACCCT CGTGGCAGGC 
CACCCCGCGC CCTACGGGGA CCTGCTGATG CAGAGCGGGG GCGCTGCCAG CGCACCCCAT 
CTCCACGACT ACCTCAACCC CGTGGACGTG TCCCGTTTCT CCAGCCCGCG GGTGACGCCC 
CGCCTGAGCC GCAAGCGGGC GCTGTCCATC TCCCCACTCT CAGACGCCAG CCTGGACCTG 
CAGCGGATGA TCCGCACCTC ACCCAACTCG CTAGTGGCCT ACATCAACAA CTCCCGAAGC 
AGCTCGGCGG CCAGCGGTTC CTACGGGCAT CTGTCAGCGG GTGCCCTCAG CCCAGCCTTC 
ACCTTCCCCC ACCCCATCAA CCCCGTGGCC TACCAGCAGA TTCTGAGCCA GCAGAGGGGT 
CTGGGGTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CCTCACCCAC CTTCCTGGCC 
CAGCAGCCCA TGGCCCTCAC CTCCATCAAT GCCACGCCCA CCCAGCTCAG CAGCAGCAGC 
AACTGTCTGA GTGACACCAA CCAGAACAAG CAGAGCAGTG AGTCGGCCGT CAGCAGCACC 
GTCAACCCTG TCGCCATTCA CAAGCGCAGC AAGGTCAAGA CCGAGCCTGA GGGCCTGCGG 
CCGGCCTCCC CTCTGGCGCT GACGCAGGAG CAGCTGGCTG ACCTCAAGGA AGATCTGGAC 
AGGGATGACT GTAAGCAGGA GGCTGAGGTG GTCATCTATG AGACCAACTG CCACTGGGAA 
GACTGCACCA AGGAGTACGA CACCCAGGAG CAGCTGGTGC ATCACATCAA CAACGAGCAC 
ATCCACGGGG AGAAGAAGGA GTTTGTGTGC CGCTGGCAGG CCTGCACGCG GGAGCAGAAG 
CCCTTCAAGG CGCAGTACAT GCTGGTGGTG CACATGCGGC GACACACGGG CGAGAAGCCC 
CACAAGTGCA CGTTCGAGGG CTGCTCGAAG GCCTACTCCC GCCTGGAGAA CCTGAAGACA 
CACCTGCGGT CCCACACCGG GGAGAAGCCA TATGTGTGTG AGCACGAGGG CTGCAACAAA 
GCCTTCTCCA ACGCCTCGGA CCGCGCCAAG CACCAGAATC GCACCCACTC CAACGAGAAA 
CCCTACATCT GCAAGATCCC AGGCTGCACC AAGAGATACA CAGACCCCAG CTCTCTCCGG 
AAGCATGTGA AAACGGTCCA CGGCCCAGAT GCCCACGTCA CCAAGAAGCA GCGCAATGAC 
GTGCACCTCC GCACACCGCT GCTCAAAGAG AATGGGGACA GTGAGGCCGG CACGGAGCCT 
GGCGGCCCAG AGAGCACCGA GGCCAGCAGC ACCAGCCAGG CCGTGGAGGA CTGCCTGCAC 
GTCAGAGCCA TCAAGACCGA GAGCTCCGGG CTGTGTCAGT CCAGCCCCGG GGCCCAGTCG 
TCCTGCAGCA GCGAGCCCTC TCCTCTGGGC AGTGCCCCCA ACAATGACAG TGGCGTGGAG 
ATGCCGGGGA CGGGGCCCGG GAGCCTGGGA GACCTGACGG CACTGGATGA CACACCCCCA 
GGGGCCGACA CCTCAGCCCT GGCTGCCCCC TCCGCTGGTG GCCTCCAGCT GCGCAAACAC 
ATGACCACCA TGCACCGGTT CGAGCAGCTC AAGAAGGAGA AGCTCAAGTC ACTCAAGGAT 
TCCTGCTCAT GGGCCGGGCC GACTCCACAC ACGCGGAACA CCAAGCTGCC TCCCCTCCCG 
GGAAGTGGCT CCATCCTGGA AAACTTCAGT GGCAGTGGGG GCGGCGGGCC CGCGGGGCTG 
CTGCCGAACC CGCGGCTGTC GGAGCTGTCC GCGAGCGAGG TGACCATGCT GAGCCAGCTG 
CAGGAGCGCC GCGACAGCTC CACCAGCACG GTCAGCTCGG CCTACACCGT GAGCCGCCGC 
TCCTCCGGCA TCTCCCCCTA CTTCTCCAGC CGCCGCTCCA GCGAGGCCTC GCCCCTGGGC 
GCCGGCCGCC CGCACAACGC GAGCTCCGCT GACTCCTACG ACCCCATCTC CACGGACGCG 
TCGCGGCGCT CGAGCGAGGC CAGCCAGTGC AGCGGCGGCT CCGGGCTGCT CAACCTCACG 
CCGGCGCAGC AGTACAGCCT GCGGGCCAAG TACGCGGCAG CCACTGGCGG CCCCCCGCCC 
ACTCCGCTGC CGGGCCTGGA GCGCATGAGC CTGCGGACCA GGCTGGCGCT GCTGGACGCG 
CCCGAGCGCA CGCTGCCCGC CGGCTGCCCA CGCCCACTGG GGCCGCGGCG TGGCAGCGAC 
GGGCCGACCT ATGGCCACGG CCACGCGGGG GCTGCGCCCG CCTTCCCCCA CGAGGCTCCA 
GGCGGCGGAG CCAGGCGGGC CAGCGACCCT GTGCGGCGGC CCGATGCCCT GTCCCTGCCG 
CGGGTGCAGC GCTTCCACAG CACCCACAAC GTGAACCCCG GCCCGCTGCC GCCCTGTGCC 
GACAGGCGAG GCCTCCGCCT GCAGAGCCAC CCGAGCACCG ACGGCGGCCT GGCCCGCGGC 
GCCTACTCGC CCCGGCCGCC TAGCATCAGC GAGAACGTGG CGATGGAGGC CGTGGCGGCA 
GGAGTGGACG GCGCGGGGCC CGAGGCCGAC CTGGGGCTGC CGGAGGACGA CCTGGTGCTT 
CCAGACGACG TGGTGCAGTA CATCAAGGCG CACGCCAGTG GCGCTCTGGA CGAGGGCACC 
GGGCAGGTGT ATCCCACGGA AAGCACTGGC TTCTCTGACA ACCCCAGACT ACCCAGCCCG 
GGGCTGCACG GCCAGCGCAG GATGGTGGCT GCGGACTCCA ACGTGGGCCC CTCCGCCCCT 
ATGCTGGGAG GATGCCAGTT AGGCTTTGGG GCGCCCTCCA GCCTGAACAA AAATAACATG 
CCTGTGCAGT GGAATGAGGT GAGCTCCGGC ACCGTAGACG CCCTGGCCAG CCAGGTGAAG 
CCTCCACCCT TTCCTCAGGG CAACCTGGCG GTGGTGCAGC AGAAGCCTGC CTTTGGCCAG 
TACCCGGGCT ACAGTCCGCA AGGCCTACAG GCTAGCCCTG GGGGCCTGGA CAGCACGCAG 
CCACACCTGC AGCCCCGCAG CGGAGCCCCC TCCCAGGGCA TCCCCAGGGT AAACTACATG 
CAGCAGCTGC GACAGCCAGT GGCAGGCAGC CAGTGTCCTG GCATGACTAC CACTATGAGC 
CCCCATGCCT GCTATGGCCA AGTCCACCCC CAGCTGAGCC CCAGCACCAT CAGTGGGGCC 
CTCAACCAGT TCCCCCAATC CTGCAGCAAC ATGCCAGCCA AGCCAGGGCA TCTGGGGCAC 
CCTCAGCAGA CAGAAGTGGC ACCTGACCCC ACCACGATGG GCAATCGCCA CAGGGAACTT 
GGGGTCCCCG ATTCAGCCCT GGCTGGAGTG CCACCACCTC ACCCAGTCCA GAGCTACCCA 
CAGCAGAGCC ATCACCTGGC AGCCTCCATG AGCCAGGAGG GCTACCACCA GGTCCCCAGC 
CTTCTGCCTG CCCGCCAGCC TGGCTTCATG GAGCCCCAAA CAGGCCCGAT GGGGGTGGCT 
ACAGCAGGCT TTGGCCTAGT GCAGCCCCGG CCTCCCCTCG AGCCCAGCCC CACTGGCCGC 
CACCGTGGGG TACGTGCTGT GCAGCAGCAG CTGGCCTACG CCAGGGCCAC AGGCCATGCC 
ATGGCTGCCA TGCCGTCCAG TCAGGAAACA GCAGAGGCTG TGCCCAAGGG AGCGATGGGC 
AACATGGGGT CGGTGCCTCC CCAGCCGCCT CCGCAGGACG CAGGTGGGGC CCCGGACCAC 
AGCATGCTCT ACTACTACGG CCAGATCCAC ATGTACGAAC AGGATGGAGG CCTGGAGAAC 
CTCGGGAGCT GCCAGGTCAT GCGGTCCCAG CCACCACAGC CACAGGCCTG TCAGGACAGC 
ATCCAGCCCC AGCCCTTGCC CTCACCAGGG GTCAACCAGG TGTCCAGCAC TGTGGACTCC 
CAGCTCCTGG AGGCCCCCCA GATTGACTTC GATGCCATCA TGGATGATGG CGATCACTCG 
AGTTTGTTCT CGGGTGCTCT GAGCCCCAGC CTCCTCCACA GCCTCTCCCA GAACTCCTCC 
CGCCTCACCA CCCCCCGAAA CTCCTTGACC CTGCCCTCCA TCCCCGCAGG CATCAGCAAC 
ATGGCTGTCG GGGACATGAG CTCCATGCTC ACCAGCCTCG CCGAGGAGAG CAAGTTCCTG 
AACATGATGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361283.1
CDS	718..5052
Translation	MEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	NM_001374354.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374354.1

ORF Insert Sequence:

ATGGAGCACT ACCTCCGTTC TGTGCACAGC AGCCCCACGC TCTCCATGAT CTCTGCAGCC 
AGGGGCCTCA GCCCCGCTGA TGTGGCCCAG GAGCACCTTA AGGAGAGGGG ACTGTTTGGC 
CTTCCTGCTC CAGGCACCAC CCCCTCAGAC TATTACCACC AGATGACCCT CGTGGCAGGC 
CACCCCGCGC CCTACGGGGA CCTGCTGATG CAGAGCGGGG GCGCTGCCAG CGCACCCCAT 
CTCCACGACT ACCTCAACCC CGTGGACGTG TCCCGTTTCT CCAGCCCGCG GGTGACGCCC 
CGCCTGAGCC GCAAGCGGGC GCTGTCCATC TCCCCACTCT CAGACGCCAG CCTGGACCTG 
CAGCGGATGA TCCGCACCTC ACCCAACTCG CTAGTGGCCT ACATCAACAA CTCCCGAAGC 
AGCTCGGCGG CCAGCGGTTC CTACGGGCAT CTGTCAGCGG GTGCCCTCAG CCCAGCCTTC 
ACCTTCCCCC ACCCCATCAA CCCCGTGGCC TACCAGCAGA TTCTGAGCCA GCAGAGGGGT 
CTGGGGTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CCTCACCCAC CTTCCTGGCC 
CAGCAGCCCA TGGCCCTCAC CTCCATCAAT GCCACGCCCA CCCAGCTCAG CAGCAGCAGC 
AACTGTCTGA GTGACACCAA CCAGAACAAG CAGAGCAGTG AGTCGGCCGT CAGCAGCACC 
GTCAACCCTG TCGCCATTCA CAAGCGCAGC AAGGTCAAGA CCGAGCCTGA GGGCCTGCGG 
CCGGCCTCCC CTCTGGCGCT GACGCAGGAG CAGCTGGCTG ACCTCAAGGA AGATCTGGAC 
AGGGATGACT GTAAGCAGGA GGCTGAGGTG GTCATCTATG AGACCAACTG CCACTGGGAA 
GACTGCACCA AGGAGTACGA CACCCAGGAG CAGCTGGTGC ATCACATCAA CAACGAGCAC 
ATCCACGGGG AGAAGAAGGA GTTTGTGTGC CGCTGGCAGG CCTGCACGCG GGAGCAGAAG 
CCCTTCAAGG CGCAGTACAT GCTGGTGGTG CACATGCGGC GACACACGGG CGAGAAGCCC 
CACAAGTGCA CGTTCGAGGG CTGCTCGAAG GCCTACTCCC GCCTGGAGAA CCTGAAGACA 
CACCTGCGGT CCCACACCGG GGAGAAGCCA TATGTGTGTG AGCACGAGGG CTGCAACAAA 
GCCTTCTCCA ACGCCTCGGA CCGCGCCAAG CACCAGAATC GCACCCACTC CAACGAGAAA 
CCCTACATCT GCAAGATCCC AGGCTGCACC AAGAGATACA CAGACCCCAG CTCTCTCCGG 
AAGCATGTGA AAACGGTCCA CGGCCCAGAT GCCCACGTCA CCAAGAAGCA GCGCAATGAC 
GTGCACCTCC GCACACCGCT GCTCAAAGAG AATGGGGACA GTGAGGCCGG CACGGAGCCT 
GGCGGCCCAG AGAGCACCGA GGCCAGCAGC ACCAGCCAGG CCGTGGAGGA CTGCCTGCAC 
GTCAGAGCCA TCAAGACCGA GAGCTCCGGG CTGTGTCAGT CCAGCCCCGG GGCCCAGTCG 
TCCTGCAGCA GCGAGCCCTC TCCTCTGGGC AGTGCCCCCA ACAATGACAG TGGCGTGGAG 
ATGCCGGGGA CGGGGCCCGG GAGCCTGGGA GACCTGACGG CACTGGATGA CACACCCCCA 
GGGGCCGACA CCTCAGCCCT GGCTGCCCCC TCCGCTGGTG GCCTCCAGCT GCGCAAACAC 
ATGACCACCA TGCACCGGTT CGAGCAGCTC AAGAAGGAGA AGCTCAAGTC ACTCAAGGAT 
TCCTGCTCAT GGGCCGGGCC GACTCCACAC ACGCGGAACA CCAAGCTGCC TCCCCTCCCG 
GGAAGTGGCT CCATCCTGGA AAACTTCAGT GGCAGTGGGG GCGGCGGGCC CGCGGGGCTG 
CTGCCGAACC CGCGGCTGTC GGAGCTGTCC GCGAGCGAGG TGACCATGCT GAGCCAGCTG 
CAGGAGCGCC GCGACAGCTC CACCAGCACG GTCAGCTCGG CCTACACCGT GAGCCGCCGC 
TCCTCCGGCA TCTCCCCCTA CTTCTCCAGC CGCCGCTCCA GCGAGGCCTC GCCCCTGGGC 
GCCGGCCGCC CGCACAACGC GAGCTCCGCT GACTCCTACG ACCCCATCTC CACGGACGCG 
TCGCGGCGCT CGAGCGAGGC CAGCCAGTGC AGCGGCGGCT CCGGGCTGCT CAACCTCACG 
CCGGCGCAGC AGTACAGCCT GCGGGCCAAG TACGCGGCAG CCACTGGCGG CCCCCCGCCC 
ACTCCGCTGC CGGGCCTGGA GCGCATGAGC CTGCGGACCA GGCTGGCGCT GCTGGACGCG 
CCCGAGCGCA CGCTGCCCGC CGGCTGCCCA CGCCCACTGG GGCCGCGGCG TGGCAGCGAC 
GGGCCGACCT ATGGCCACGG CCACGCGGGG GCTGCGCCCG CCTTCCCCCA CGAGGCTCCA 
GGCGGCGGAG CCAGGCGGGC CAGCGACCCT GTGCGGCGGC CCGATGCCCT GTCCCTGCCG 
CGGGTGCAGC GCTTCCACAG CACCCACAAC GTGAACCCCG GCCCGCTGCC GCCCTGTGCC 
GACAGGCGAG GCCTCCGCCT GCAGAGCCAC CCGAGCACCG ACGGCGGCCT GGCCCGCGGC 
GCCTACTCGC CCCGGCCGCC TAGCATCAGC GAGAACGTGG CGATGGAGGC CGTGGCGGCA 
GGAGTGGACG GCGCGGGGCC CGAGGCCGAC CTGGGGCTGC CGGAGGACGA CCTGGTGCTT 
CCAGACGACG TGGTGCAGTA CATCAAGGCG CACGCCAGTG GCGCTCTGGA CGAGGGCACC 
GGGCAGGTGT ATCCCACGGA AAGCACTGGC TTCTCTGACA ACCCCAGACT ACCCAGCCCG 
GGGCTGCACG GCCAGCGCAG GATGGTGGCT GCGGACTCCA ACGTGGGCCC CTCCGCCCCT 
ATGCTGGGAG GATGCCAGTT AGGCTTTGGG GCGCCCTCCA GCCTGAACAA AAATAACATG 
CCTGTGCAGT GGAATGAGGT GAGCTCCGGC ACCGTAGACG CCCTGGCCAG CCAGGTGAAG 
CCTCCACCCT TTCCTCAGGG CAACCTGGCG GTGGTGCAGC AGAAGCCTGC CTTTGGCCAG 
TACCCGGGCT ACAGTCCGCA AGGCCTACAG GCTAGCCCTG GGGGCCTGGA CAGCACGCAG 
CCACACCTGC AGCCCCGCAG CGGAGCCCCC TCCCAGGGCA TCCCCAGGGT AAACTACATG 
CAGCAGCTGC GACAGCCAGT GGCAGGCAGC CAGTGTCCTG GCATGACTAC CACTATGAGC 
CCCCATGCCT GCTATGGCCA AGTCCACCCC CAGCTGAGCC CCAGCACCAT CAGTGGGGCC 
CTCAACCAGT TCCCCCAATC CTGCAGCAAC ATGCCAGCCA AGCCAGGGCA TCTGGGGCAC 
CCTCAGCAGA CAGAAGTGGC ACCTGACCCC ACCACGATGG GCAATCGCCA CAGGGAACTT 
GGGGTCCCCG ATTCAGCCCT GGCTGGAGTG CCACCACCTC ACCCAGTCCA GAGCTACCCA 
CAGCAGAGCC ATCACCTGGC AGCCTCCATG AGCCAGGAGG GCTACCACCA GGTCCCCAGC 
CTTCTGCCTG CCCGCCAGCC TGGCTTCATG GAGCCCCAAA CAGGCCCGAT GGGGGTGGCT 
ACAGCAGGCT TTGGCCTAGT GCAGCCCCGG CCTCCCCTCG AGCCCAGCCC CACTGGCCGC 
CACCGTGGGG TACGTGCTGT GCAGCAGCAG CTGGCCTACG CCAGGGCCAC AGGCCATGCC 
ATGGCTGCCA TGCCGTCCAG TCAGGAAACA GCAGAGGCTG TGCCCAAGGG AGCGATGGGC 
AACATGGGGT CGGTGCCTCC CCAGCCGCCT CCGCAGGACG CAGGTGGGGC CCCGGACCAC 
AGCATGCTCT ACTACTACGG CCAGATCCAC ATGTACGAAC AGGATGGAGG CCTGGAGAAC 
CTCGGGAGCT GCCAGGTCAT GCGGTCCCAG CCACCACAGC CACAGGCCTG TCAGGACAGC 
ATCCAGCCCC AGCCCTTGCC CTCACCAGGG GTCAACCAGG TGTCCAGCAC TGTGGACTCC 
CAGCTCCTGG AGGCCCCCCA GATTGACTTC GATGCCATCA TGGATGATGG CGATCACTCG 
AGTTTGTTCT CGGGTGCTCT GAGCCCCAGC CTCCTCCACA GCCTCTCCCA GAACTCCTCC 
CGCCTCACCA CCCCCCGAAA CTCCTTGACC CTGCCCTCCA TCCCCGCAGG CATCAGCAAC 
ATGGCTGTCG GGGACATGAG CTCCATGCTC ACCAGCCTCG CCGAGGAGAG CAAGTTCCTG 
AACATGATGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( NM_001374353.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361282.1 Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu36103
Clone ID Related Accession (Same CDS sequence)	NM_001374353.1 , XM_006712422.2 , XM_006712422.3
Accession Version	NM_001374353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	zinc finger protein GLI2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC017033.5 and AC016764.8. On Sep 23, 2019 this sequence version replaced XM_006712422.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG 
GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG 
GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG 
CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC 
CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG 
ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG 
TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC 
ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG 
AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG 
ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT 
GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC 
CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC 
GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC 
AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC 
CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG 
AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA 
CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG 
CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG 
GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG 
CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGAGCAGCT GGCTGACCTC 
AAGGAAGATC TGGACAGGGA TGACTGTAAG CAGGAGGCTG AGGTGGTCAT CTATGAGACC 
AACTGCCACT GGGAAGACTG CACCAAGGAG TACGACACCC AGGAGCAGCT GGTGCATCAC 
ATCAACAACG AGCACATCCA CGGGGAGAAG AAGGAGTTTG TGTGCCGCTG GCAGGCCTGC 
ACGCGGGAGC AGAAGCCCTT CAAGGCGCAG TACATGCTGG TGGTGCACAT GCGGCGACAC 
ACGGGCGAGA AGCCCCACAA GTGCACGTTC GAGGGCTGCT CGAAGGCCTA CTCCCGCCTG 
GAGAACCTGA AGACACACCT GCGGTCCCAC ACCGGGGAGA AGCCATATGT GTGTGAGCAC 
GAGGGCTGCA ACAAAGCCTT CTCCAACGCC TCGGACCGCG CCAAGCACCA GAATCGCACC 
CACTCCAACG AGAAACCCTA CATCTGCAAG ATCCCAGGCT GCACCAAGAG ATACACAGAC 
CCCAGCTCTC TCCGGAAGCA TGTGAAAACG GTCCACGGCC CAGATGCCCA CGTCACCAAG 
AAGCAGCGCA ATGACGTGCA CCTCCGCACA CCGCTGCTCA AAGAGAATGG GGACAGTGAG 
GCCGGCACGG AGCCTGGCGG CCCAGAGAGC ACCGAGGCCA GCAGCACCAG CCAGGCCGTG 
GAGGACTGCC TGCACGTCAG AGCCATCAAG ACCGAGAGCT CCGGGCTGTG TCAGTCCAGC 
CCCGGGGCCC AGTCGTCCTG CAGCAGCGAG CCCTCTCCTC TGGGCAGTGC CCCCAACAAT 
GACAGTGGCG TGGAGATGCC GGGGACGGGG CCCGGGAGCC TGGGAGACCT GACGGCACTG 
GATGACACAC CCCCAGGGGC CGACACCTCA GCCCTGGCTG CCCCCTCCGC TGGTGGCCTC 
CAGCTGCGCA AACACATGAC CACCATGCAC CGGTTCGAGC AGCTCAAGAA GGAGAAGCTC 
AAGTCACTCA AGGATTCCTG CTCATGGGCC GGGCCGACTC CACACACGCG GAACACCAAG 
CTGCCTCCCC TCCCGGGAAG TGGCTCCATC CTGGAAAACT TCAGTGGCAG TGGGGGCGGC 
GGGCCCGCGG GGCTGCTGCC GAACCCGCGG CTGTCGGAGC TGTCCGCGAG CGAGGTGACC 
ATGCTGAGCC AGCTGCAGGA GCGCCGCGAC AGCTCCACCA GCACGGTCAG CTCGGCCTAC 
ACCGTGAGCC GCCGCTCCTC CGGCATCTCC CCCTACTTCT CCAGCCGCCG CTCCAGCGAG 
GCCTCGCCCC TGGGCGCCGG CCGCCCGCAC AACGCGAGCT CCGCTGACTC CTACGACCCC 
ATCTCCACGG ACGCGTCGCG GCGCTCGAGC GAGGCCAGCC AGTGCAGCGG CGGCTCCGGG 
CTGCTCAACC TCACGCCGGC GCAGCAGTAC AGCCTGCGGG CCAAGTACGC GGCAGCCACT 
GGCGGCCCCC CGCCCACTCC GCTGCCGGGC CTGGAGCGCA TGAGCCTGCG GACCAGGCTG 
GCGCTGCTGG ACGCGCCCGA GCGCACGCTG CCCGCCGGCT GCCCACGCCC ACTGGGGCCG 
CGGCGTGGCA GCGACGGGCC GACCTATGGC CACGGCCACG CGGGGGCTGC GCCCGCCTTC 
CCCCACGAGG CTCCAGGCGG CGGAGCCAGG CGGGCCAGCG ACCCTGTGCG GCGGCCCGAT 
GCCCTGTCCC TGCCGCGGGT GCAGCGCTTC CACAGCACCC ACAACGTGAA CCCCGGCCCG 
CTGCCGCCCT GTGCCGACAG GCGAGGCCTC CGCCTGCAGA GCCACCCGAG CACCGACGGC 
GGCCTGGCCC GCGGCGCCTA CTCGCCCCGG CCGCCTAGCA TCAGCGAGAA CGTGGCGATG 
GAGGCCGTGG CGGCAGGAGT GGACGGCGCG GGGCCCGAGG CCGACCTGGG GCTGCCGGAG 
GACGACCTGG TGCTTCCAGA CGACGTGGTG CAGTACATCA AGGCGCACGC CAGTGGCGCT 
CTGGACGAGG GCACCGGGCA GGTGTATCCC ACGGAAAGCA CTGGCTTCTC TGACAACCCC 
AGACTACCCA GCCCGGGGCT GCACGGCCAG CGCAGGATGG TGGCTGCGGA CTCCAACGTG 
GGCCCCTCCG CCCCTATGCT GGGAGGATGC CAGTTAGGCT TTGGGGCGCC CTCCAGCCTG 
AACAAAAATA ACATGCCTGT GCAGTGGAAT GAGGTGAGCT CCGGCACCGT AGACGCCCTG 
GCCAGCCAGG TGAAGCCTCC ACCCTTTCCT CAGGGCAACC TGGCGGTGGT GCAGCAGAAG 
CCTGCCTTTG GCCAGTACCC GGGCTACAGT CCGCAAGGCC TACAGGCTAG CCCTGGGGGC 
CTGGACAGCA CGCAGCCACA CCTGCAGCCC CGCAGCGGAG CCCCCTCCCA GGGCATCCCC 
AGGGTAAACT ACATGCAGCA GCTGCGACAG CCAGTGGCAG GCAGCCAGTG TCCTGGCATG 
ACTACCACTA TGAGCCCCCA TGCCTGCTAT GGCCAAGTCC ACCCCCAGCT GAGCCCCAGC 
ACCATCAGTG GGGCCCTCAA CCAGTTCCCC CAATCCTGCA GCAACATGCC AGCCAAGCCA 
GGGCATCTGG GGCACCCTCA GCAGACAGAA GTGGCACCTG ACCCCACCAC GATGGGCAAT 
CGCCACAGGG AACTTGGGGT CCCCGATTCA GCCCTGGCTG GAGTGCCACC ACCTCACCCA 
GTCCAGAGCT ACCCACAGCA GAGCCATCAC CTGGCAGCCT CCATGAGCCA GGAGGGCTAC 
CACCAGGTCC CCAGCCTTCT GCCTGCCCGC CAGCCTGGCT TCATGGAGCC CCAAACAGGC 
CCGATGGGGG TGGCTACAGC AGGCTTTGGC CTAGTGCAGC CCCGGCCTCC CCTCGAGCCC 
AGCCCCACTG GCCGCCACCG TGGGGTACGT GCTGTGCAGC AGCAGCTGGC CTACGCCAGG 
GCCACAGGCC ATGCCATGGC TGCCATGCCG TCCAGTCAGG AAACAGCAGA GGCTGTGCCC 
AAGGGAGCGA TGGGCAACAT GGGGTCGGTG CCTCCCCAGC CGCCTCCGCA GGACGCAGGT 
GGGGCCCCGG ACCACAGCAT GCTCTACTAC TACGGCCAGA TCCACATGTA CGAACAGGAT 
GGAGGCCTGG AGAACCTCGG GAGCTGCCAG GTCATGCGGT CCCAGCCACC ACAGCCACAG 
GCCTGTCAGG ACAGCATCCA GCCCCAGCCC TTGCCCTCAC CAGGGGTCAA CCAGGTGTCC 
AGCACTGTGG ACTCCCAGCT CCTGGAGGCC CCCCAGATTG ACTTCGATGC CATCATGGAT 
GATGGCGATC ACTCGAGTTT GTTCTCGGGT GCTCTGAGCC CCAGCCTCCT CCACAGCCTC 
TCCCAGAACT CCTCCCGCCT CACCACCCCC CGAAACTCCT TGACCCTGCC CTCCATCCCC 
GCAGGCATCA GCAACATGGC TGTCGGGGAC ATGAGCTCCA TGCTCACCAG CCTCGCCGAG 
GAGAGCAAGT TCCTGAACAT GATGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361282.1
CDS	449..5158
Translation	METSASATASEKQEAKSGILEAAGFPDPGKKASPLVVAAAAAAAVAAQGVPQHLLPPFHAPLPIDMRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	NM_001374353.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374353.1

ORF Insert Sequence:

ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG 
GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG 
GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG 
CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC 
CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG 
ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG 
TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC 
ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG 
AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG 
ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT 
GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC 
CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC 
GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC 
AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC 
CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG 
AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA 
CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG 
CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG 
GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG 
CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGAGCAGCT GGCTGACCTC 
AAGGAAGATC TGGACAGGGA TGACTGTAAG CAGGAGGCTG AGGTGGTCAT CTATGAGACC 
AACTGCCACT GGGAAGACTG CACCAAGGAG TACGACACCC AGGAGCAGCT GGTGCATCAC 
ATCAACAACG AGCACATCCA CGGGGAGAAG AAGGAGTTTG TGTGCCGCTG GCAGGCCTGC 
ACGCGGGAGC AGAAGCCCTT CAAGGCGCAG TACATGCTGG TGGTGCACAT GCGGCGACAC 
ACGGGCGAGA AGCCCCACAA GTGCACGTTC GAGGGCTGCT CGAAGGCCTA CTCCCGCCTG 
GAGAACCTGA AGACACACCT GCGGTCCCAC ACCGGGGAGA AGCCATATGT GTGTGAGCAC 
GAGGGCTGCA ACAAAGCCTT CTCCAACGCC TCGGACCGCG CCAAGCACCA GAATCGCACC 
CACTCCAACG AGAAACCCTA CATCTGCAAG ATCCCAGGCT GCACCAAGAG ATACACAGAC 
CCCAGCTCTC TCCGGAAGCA TGTGAAAACG GTCCACGGCC CAGATGCCCA CGTCACCAAG 
AAGCAGCGCA ATGACGTGCA CCTCCGCACA CCGCTGCTCA AAGAGAATGG GGACAGTGAG 
GCCGGCACGG AGCCTGGCGG CCCAGAGAGC ACCGAGGCCA GCAGCACCAG CCAGGCCGTG 
GAGGACTGCC TGCACGTCAG AGCCATCAAG ACCGAGAGCT CCGGGCTGTG TCAGTCCAGC 
CCCGGGGCCC AGTCGTCCTG CAGCAGCGAG CCCTCTCCTC TGGGCAGTGC CCCCAACAAT 
GACAGTGGCG TGGAGATGCC GGGGACGGGG CCCGGGAGCC TGGGAGACCT GACGGCACTG 
GATGACACAC CCCCAGGGGC CGACACCTCA GCCCTGGCTG CCCCCTCCGC TGGTGGCCTC 
CAGCTGCGCA AACACATGAC CACCATGCAC CGGTTCGAGC AGCTCAAGAA GGAGAAGCTC 
AAGTCACTCA AGGATTCCTG CTCATGGGCC GGGCCGACTC CACACACGCG GAACACCAAG 
CTGCCTCCCC TCCCGGGAAG TGGCTCCATC CTGGAAAACT TCAGTGGCAG TGGGGGCGGC 
GGGCCCGCGG GGCTGCTGCC GAACCCGCGG CTGTCGGAGC TGTCCGCGAG CGAGGTGACC 
ATGCTGAGCC AGCTGCAGGA GCGCCGCGAC AGCTCCACCA GCACGGTCAG CTCGGCCTAC 
ACCGTGAGCC GCCGCTCCTC CGGCATCTCC CCCTACTTCT CCAGCCGCCG CTCCAGCGAG 
GCCTCGCCCC TGGGCGCCGG CCGCCCGCAC AACGCGAGCT CCGCTGACTC CTACGACCCC 
ATCTCCACGG ACGCGTCGCG GCGCTCGAGC GAGGCCAGCC AGTGCAGCGG CGGCTCCGGG 
CTGCTCAACC TCACGCCGGC GCAGCAGTAC AGCCTGCGGG CCAAGTACGC GGCAGCCACT 
GGCGGCCCCC CGCCCACTCC GCTGCCGGGC CTGGAGCGCA TGAGCCTGCG GACCAGGCTG 
GCGCTGCTGG ACGCGCCCGA GCGCACGCTG CCCGCCGGCT GCCCACGCCC ACTGGGGCCG 
CGGCGTGGCA GCGACGGGCC GACCTATGGC CACGGCCACG CGGGGGCTGC GCCCGCCTTC 
CCCCACGAGG CTCCAGGCGG CGGAGCCAGG CGGGCCAGCG ACCCTGTGCG GCGGCCCGAT 
GCCCTGTCCC TGCCGCGGGT GCAGCGCTTC CACAGCACCC ACAACGTGAA CCCCGGCCCG 
CTGCCGCCCT GTGCCGACAG GCGAGGCCTC CGCCTGCAGA GCCACCCGAG CACCGACGGC 
GGCCTGGCCC GCGGCGCCTA CTCGCCCCGG CCGCCTAGCA TCAGCGAGAA CGTGGCGATG 
GAGGCCGTGG CGGCAGGAGT GGACGGCGCG GGGCCCGAGG CCGACCTGGG GCTGCCGGAG 
GACGACCTGG TGCTTCCAGA CGACGTGGTG CAGTACATCA AGGCGCACGC CAGTGGCGCT 
CTGGACGAGG GCACCGGGCA GGTGTATCCC ACGGAAAGCA CTGGCTTCTC TGACAACCCC 
AGACTACCCA GCCCGGGGCT GCACGGCCAG CGCAGGATGG TGGCTGCGGA CTCCAACGTG 
GGCCCCTCCG CCCCTATGCT GGGAGGATGC CAGTTAGGCT TTGGGGCGCC CTCCAGCCTG 
AACAAAAATA ACATGCCTGT GCAGTGGAAT GAGGTGAGCT CCGGCACCGT AGACGCCCTG 
GCCAGCCAGG TGAAGCCTCC ACCCTTTCCT CAGGGCAACC TGGCGGTGGT GCAGCAGAAG 
CCTGCCTTTG GCCAGTACCC GGGCTACAGT CCGCAAGGCC TACAGGCTAG CCCTGGGGGC 
CTGGACAGCA CGCAGCCACA CCTGCAGCCC CGCAGCGGAG CCCCCTCCCA GGGCATCCCC 
AGGGTAAACT ACATGCAGCA GCTGCGACAG CCAGTGGCAG GCAGCCAGTG TCCTGGCATG 
ACTACCACTA TGAGCCCCCA TGCCTGCTAT GGCCAAGTCC ACCCCCAGCT GAGCCCCAGC 
ACCATCAGTG GGGCCCTCAA CCAGTTCCCC CAATCCTGCA GCAACATGCC AGCCAAGCCA 
GGGCATCTGG GGCACCCTCA GCAGACAGAA GTGGCACCTG ACCCCACCAC GATGGGCAAT 
CGCCACAGGG AACTTGGGGT CCCCGATTCA GCCCTGGCTG GAGTGCCACC ACCTCACCCA 
GTCCAGAGCT ACCCACAGCA GAGCCATCAC CTGGCAGCCT CCATGAGCCA GGAGGGCTAC 
CACCAGGTCC CCAGCCTTCT GCCTGCCCGC CAGCCTGGCT TCATGGAGCC CCAAACAGGC 
CCGATGGGGG TGGCTACAGC AGGCTTTGGC CTAGTGCAGC CCCGGCCTCC CCTCGAGCCC 
AGCCCCACTG GCCGCCACCG TGGGGTACGT GCTGTGCAGC AGCAGCTGGC CTACGCCAGG 
GCCACAGGCC ATGCCATGGC TGCCATGCCG TCCAGTCAGG AAACAGCAGA GGCTGTGCCC 
AAGGGAGCGA TGGGCAACAT GGGGTCGGTG CCTCCCCAGC CGCCTCCGCA GGACGCAGGT 
GGGGCCCCGG ACCACAGCAT GCTCTACTAC TACGGCCAGA TCCACATGTA CGAACAGGAT 
GGAGGCCTGG AGAACCTCGG GAGCTGCCAG GTCATGCGGT CCCAGCCACC ACAGCCACAG 
GCCTGTCAGG ACAGCATCCA GCCCCAGCCC TTGCCCTCAC CAGGGGTCAA CCAGGTGTCC 
AGCACTGTGG ACTCCCAGCT CCTGGAGGCC CCCCAGATTG ACTTCGATGC CATCATGGAT 
GATGGCGATC ACTCGAGTTT GTTCTCGGGT GCTCTGAGCC CCAGCCTCCT CCACAGCCTC 
TCCCAGAACT CCTCCCGCCT CACCACCCCC CGAAACTCCT TGACCCTGCC CTCCATCCCC 
GCAGGCATCA GCAACATGGC TGTCGGGGAC ATGAGCTCCA TGCTCACCAG CCTCGCCGAG 
GAGAGCAAGT TCCTGAACAT GATGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( XM_006712422.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712485.1 Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X3, mRNA.

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CloneID	OHu36103
Clone ID Related Accession (Same CDS sequence)	NM_001374353.1 , XM_006712422.2 , XM_006712422.3
Accession Version	XM_006712422.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	zinc finger protein GLI2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG 
GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG 
GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG 
CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC 
CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG 
ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG 
TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC 
ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG 
AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG 
ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT 
GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC 
CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC 
GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC 
AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC 
CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG 
AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA 
CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG 
CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG 
GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG 
CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGAGCAGCT GGCTGACCTC 
AAGGAAGATC TGGACAGGGA TGACTGTAAG CAGGAGGCTG AGGTGGTCAT CTATGAGACC 
AACTGCCACT GGGAAGACTG CACCAAGGAG TACGACACCC AGGAGCAGCT GGTGCATCAC 
ATCAACAACG AGCACATCCA CGGGGAGAAG AAGGAGTTTG TGTGCCGCTG GCAGGCCTGC 
ACGCGGGAGC AGAAGCCCTT CAAGGCGCAG TACATGCTGG TGGTGCACAT GCGGCGACAC 
ACGGGCGAGA AGCCCCACAA GTGCACGTTC GAGGGCTGCT CGAAGGCCTA CTCCCGCCTG 
GAGAACCTGA AGACACACCT GCGGTCCCAC ACCGGGGAGA AGCCATATGT GTGTGAGCAC 
GAGGGCTGCA ACAAAGCCTT CTCCAACGCC TCGGACCGCG CCAAGCACCA GAATCGCACC 
CACTCCAACG AGAAACCCTA CATCTGCAAG ATCCCAGGCT GCACCAAGAG ATACACAGAC 
CCCAGCTCTC TCCGGAAGCA TGTGAAAACG GTCCACGGCC CAGATGCCCA CGTCACCAAG 
AAGCAGCGCA ATGACGTGCA CCTCCGCACA CCGCTGCTCA AAGAGAATGG GGACAGTGAG 
GCCGGCACGG AGCCTGGCGG CCCAGAGAGC ACCGAGGCCA GCAGCACCAG CCAGGCCGTG 
GAGGACTGCC TGCACGTCAG AGCCATCAAG ACCGAGAGCT CCGGGCTGTG TCAGTCCAGC 
CCCGGGGCCC AGTCGTCCTG CAGCAGCGAG CCCTCTCCTC TGGGCAGTGC CCCCAACAAT 
GACAGTGGCG TGGAGATGCC GGGGACGGGG CCCGGGAGCC TGGGAGACCT GACGGCACTG 
GATGACACAC CCCCAGGGGC CGACACCTCA GCCCTGGCTG CCCCCTCCGC TGGTGGCCTC 
CAGCTGCGCA AACACATGAC CACCATGCAC CGGTTCGAGC AGCTCAAGAA GGAGAAGCTC 
AAGTCACTCA AGGATTCCTG CTCATGGGCC GGGCCGACTC CACACACGCG GAACACCAAG 
CTGCCTCCCC TCCCGGGAAG TGGCTCCATC CTGGAAAACT TCAGTGGCAG TGGGGGCGGC 
GGGCCCGCGG GGCTGCTGCC GAACCCGCGG CTGTCGGAGC TGTCCGCGAG CGAGGTGACC 
ATGCTGAGCC AGCTGCAGGA GCGCCGCGAC AGCTCCACCA GCACGGTCAG CTCGGCCTAC 
ACCGTGAGCC GCCGCTCCTC CGGCATCTCC CCCTACTTCT CCAGCCGCCG CTCCAGCGAG 
GCCTCGCCCC TGGGCGCCGG CCGCCCGCAC AACGCGAGCT CCGCTGACTC CTACGACCCC 
ATCTCCACGG ACGCGTCGCG GCGCTCGAGC GAGGCCAGCC AGTGCAGCGG CGGCTCCGGG 
CTGCTCAACC TCACGCCGGC GCAGCAGTAC AGCCTGCGGG CCAAGTACGC GGCAGCCACT 
GGCGGCCCCC CGCCCACTCC GCTGCCGGGC CTGGAGCGCA TGAGCCTGCG GACCAGGCTG 
GCGCTGCTGG ACGCGCCCGA GCGCACGCTG CCCGCCGGCT GCCCACGCCC ACTGGGGCCG 
CGGCGTGGCA GCGACGGGCC GACCTATGGC CACGGCCACG CGGGGGCTGC GCCCGCCTTC 
CCCCACGAGG CTCCAGGCGG CGGAGCCAGG CGGGCCAGCG ACCCTGTGCG GCGGCCCGAT 
GCCCTGTCCC TGCCGCGGGT GCAGCGCTTC CACAGCACCC ACAACGTGAA CCCCGGCCCG 
CTGCCGCCCT GTGCCGACAG GCGAGGCCTC CGCCTGCAGA GCCACCCGAG CACCGACGGC 
GGCCTGGCCC GCGGCGCCTA CTCGCCCCGG CCGCCTAGCA TCAGCGAGAA CGTGGCGATG 
GAGGCCGTGG CGGCAGGAGT GGACGGCGCG GGGCCCGAGG CCGACCTGGG GCTGCCGGAG 
GACGACCTGG TGCTTCCAGA CGACGTGGTG CAGTACATCA AGGCGCACGC CAGTGGCGCT 
CTGGACGAGG GCACCGGGCA GGTGTATCCC ACGGAAAGCA CTGGCTTCTC TGACAACCCC 
AGACTACCCA GCCCGGGGCT GCACGGCCAG CGCAGGATGG TGGCTGCGGA CTCCAACGTG 
GGCCCCTCCG CCCCTATGCT GGGAGGATGC CAGTTAGGCT TTGGGGCGCC CTCCAGCCTG 
AACAAAAATA ACATGCCTGT GCAGTGGAAT GAGGTGAGCT CCGGCACCGT AGACGCCCTG 
GCCAGCCAGG TGAAGCCTCC ACCCTTTCCT CAGGGCAACC TGGCGGTGGT GCAGCAGAAG 
CCTGCCTTTG GCCAGTACCC GGGCTACAGT CCGCAAGGCC TACAGGCTAG CCCTGGGGGC 
CTGGACAGCA CGCAGCCACA CCTGCAGCCC CGCAGCGGAG CCCCCTCCCA GGGCATCCCC 
AGGGTAAACT ACATGCAGCA GCTGCGACAG CCAGTGGCAG GCAGCCAGTG TCCTGGCATG 
ACTACCACTA TGAGCCCCCA TGCCTGCTAT GGCCAAGTCC ACCCCCAGCT GAGCCCCAGC 
ACCATCAGTG GGGCCCTCAA CCAGTTCCCC CAATCCTGCA GCAACATGCC AGCCAAGCCA 
GGGCATCTGG GGCACCCTCA GCAGACAGAA GTGGCACCTG ACCCCACCAC GATGGGCAAT 
CGCCACAGGG AACTTGGGGT CCCCGATTCA GCCCTGGCTG GAGTGCCACC ACCTCACCCA 
GTCCAGAGCT ACCCACAGCA GAGCCATCAC CTGGCAGCCT CCATGAGCCA GGAGGGCTAC 
CACCAGGTCC CCAGCCTTCT GCCTGCCCGC CAGCCTGGCT TCATGGAGCC CCAAACAGGC 
CCGATGGGGG TGGCTACAGC AGGCTTTGGC CTAGTGCAGC CCCGGCCTCC CCTCGAGCCC 
AGCCCCACTG GCCGCCACCG TGGGGTACGT GCTGTGCAGC AGCAGCTGGC CTACGCCAGG 
GCCACAGGCC ATGCCATGGC TGCCATGCCG TCCAGTCAGG AAACAGCAGA GGCTGTGCCC 
AAGGGAGCGA TGGGCAACAT GGGGTCGGTG CCTCCCCAGC CGCCTCCGCA GGACGCAGGT 
GGGGCCCCGG ACCACAGCAT GCTCTACTAC TACGGCCAGA TCCACATGTA CGAACAGGAT 
GGAGGCCTGG AGAACCTCGG GAGCTGCCAG GTCATGCGGT CCCAGCCACC ACAGCCACAG 
GCCTGTCAGG ACAGCATCCA GCCCCAGCCC TTGCCCTCAC CAGGGGTCAA CCAGGTGTCC 
AGCACTGTGG ACTCCCAGCT CCTGGAGGCC CCCCAGATTG ACTTCGATGC CATCATGGAT 
GATGGCGATC ACTCGAGTTT GTTCTCGGGT GCTCTGAGCC CCAGCCTCCT CCACAGCCTC 
TCCCAGAACT CCTCCCGCCT CACCACCCCC CGAAACTCCT TGACCCTGCC CTCCATCCCC 
GCAGGCATCA GCAACATGGC TGTCGGGGAC ATGAGCTCCA TGCTCACCAG CCTCGCCGAG 
GAGAGCAAGT TCCTGAACAT GATGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712485.1
CDS	31..4740
Translation	METSASATASEKQEAKSGILEAAGFPDPGKKASPLVVAAAAAAAVAAQGVPQHLLPPFHAPLPIDMRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	XM_006712422.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712422.2

ORF Insert Sequence:

ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG 
GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG 
GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG 
CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC 
CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG 
ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG 
TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC 
ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG 
AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG 
ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT 
GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC 
CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC 
GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC 
AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC 
CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG 
AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA 
CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG 
CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG 
GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG 
CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGAGCAGCT GGCTGACCTC 
AAGGAAGATC TGGACAGGGA TGACTGTAAG CAGGAGGCTG AGGTGGTCAT CTATGAGACC 
AACTGCCACT GGGAAGACTG CACCAAGGAG TACGACACCC AGGAGCAGCT GGTGCATCAC 
ATCAACAACG AGCACATCCA CGGGGAGAAG AAGGAGTTTG TGTGCCGCTG GCAGGCCTGC 
ACGCGGGAGC AGAAGCCCTT CAAGGCGCAG TACATGCTGG TGGTGCACAT GCGGCGACAC 
ACGGGCGAGA AGCCCCACAA GTGCACGTTC GAGGGCTGCT CGAAGGCCTA CTCCCGCCTG 
GAGAACCTGA AGACACACCT GCGGTCCCAC ACCGGGGAGA AGCCATATGT GTGTGAGCAC 
GAGGGCTGCA ACAAAGCCTT CTCCAACGCC TCGGACCGCG CCAAGCACCA GAATCGCACC 
CACTCCAACG AGAAACCCTA CATCTGCAAG ATCCCAGGCT GCACCAAGAG ATACACAGAC 
CCCAGCTCTC TCCGGAAGCA TGTGAAAACG GTCCACGGCC CAGATGCCCA CGTCACCAAG 
AAGCAGCGCA ATGACGTGCA CCTCCGCACA CCGCTGCTCA AAGAGAATGG GGACAGTGAG 
GCCGGCACGG AGCCTGGCGG CCCAGAGAGC ACCGAGGCCA GCAGCACCAG CCAGGCCGTG 
GAGGACTGCC TGCACGTCAG AGCCATCAAG ACCGAGAGCT CCGGGCTGTG TCAGTCCAGC 
CCCGGGGCCC AGTCGTCCTG CAGCAGCGAG CCCTCTCCTC TGGGCAGTGC CCCCAACAAT 
GACAGTGGCG TGGAGATGCC GGGGACGGGG CCCGGGAGCC TGGGAGACCT GACGGCACTG 
GATGACACAC CCCCAGGGGC CGACACCTCA GCCCTGGCTG CCCCCTCCGC TGGTGGCCTC 
CAGCTGCGCA AACACATGAC CACCATGCAC CGGTTCGAGC AGCTCAAGAA GGAGAAGCTC 
AAGTCACTCA AGGATTCCTG CTCATGGGCC GGGCCGACTC CACACACGCG GAACACCAAG 
CTGCCTCCCC TCCCGGGAAG TGGCTCCATC CTGGAAAACT TCAGTGGCAG TGGGGGCGGC 
GGGCCCGCGG GGCTGCTGCC GAACCCGCGG CTGTCGGAGC TGTCCGCGAG CGAGGTGACC 
ATGCTGAGCC AGCTGCAGGA GCGCCGCGAC AGCTCCACCA GCACGGTCAG CTCGGCCTAC 
ACCGTGAGCC GCCGCTCCTC CGGCATCTCC CCCTACTTCT CCAGCCGCCG CTCCAGCGAG 
GCCTCGCCCC TGGGCGCCGG CCGCCCGCAC AACGCGAGCT CCGCTGACTC CTACGACCCC 
ATCTCCACGG ACGCGTCGCG GCGCTCGAGC GAGGCCAGCC AGTGCAGCGG CGGCTCCGGG 
CTGCTCAACC TCACGCCGGC GCAGCAGTAC AGCCTGCGGG CCAAGTACGC GGCAGCCACT 
GGCGGCCCCC CGCCCACTCC GCTGCCGGGC CTGGAGCGCA TGAGCCTGCG GACCAGGCTG 
GCGCTGCTGG ACGCGCCCGA GCGCACGCTG CCCGCCGGCT GCCCACGCCC ACTGGGGCCG 
CGGCGTGGCA GCGACGGGCC GACCTATGGC CACGGCCACG CGGGGGCTGC GCCCGCCTTC 
CCCCACGAGG CTCCAGGCGG CGGAGCCAGG CGGGCCAGCG ACCCTGTGCG GCGGCCCGAT 
GCCCTGTCCC TGCCGCGGGT GCAGCGCTTC CACAGCACCC ACAACGTGAA CCCCGGCCCG 
CTGCCGCCCT GTGCCGACAG GCGAGGCCTC CGCCTGCAGA GCCACCCGAG CACCGACGGC 
GGCCTGGCCC GCGGCGCCTA CTCGCCCCGG CCGCCTAGCA TCAGCGAGAA CGTGGCGATG 
GAGGCCGTGG CGGCAGGAGT GGACGGCGCG GGGCCCGAGG CCGACCTGGG GCTGCCGGAG 
GACGACCTGG TGCTTCCAGA CGACGTGGTG CAGTACATCA AGGCGCACGC CAGTGGCGCT 
CTGGACGAGG GCACCGGGCA GGTGTATCCC ACGGAAAGCA CTGGCTTCTC TGACAACCCC 
AGACTACCCA GCCCGGGGCT GCACGGCCAG CGCAGGATGG TGGCTGCGGA CTCCAACGTG 
GGCCCCTCCG CCCCTATGCT GGGAGGATGC CAGTTAGGCT TTGGGGCGCC CTCCAGCCTG 
AACAAAAATA ACATGCCTGT GCAGTGGAAT GAGGTGAGCT CCGGCACCGT AGACGCCCTG 
GCCAGCCAGG TGAAGCCTCC ACCCTTTCCT CAGGGCAACC TGGCGGTGGT GCAGCAGAAG 
CCTGCCTTTG GCCAGTACCC GGGCTACAGT CCGCAAGGCC TACAGGCTAG CCCTGGGGGC 
CTGGACAGCA CGCAGCCACA CCTGCAGCCC CGCAGCGGAG CCCCCTCCCA GGGCATCCCC 
AGGGTAAACT ACATGCAGCA GCTGCGACAG CCAGTGGCAG GCAGCCAGTG TCCTGGCATG 
ACTACCACTA TGAGCCCCCA TGCCTGCTAT GGCCAAGTCC ACCCCCAGCT GAGCCCCAGC 
ACCATCAGTG GGGCCCTCAA CCAGTTCCCC CAATCCTGCA GCAACATGCC AGCCAAGCCA 
GGGCATCTGG GGCACCCTCA GCAGACAGAA GTGGCACCTG ACCCCACCAC GATGGGCAAT 
CGCCACAGGG AACTTGGGGT CCCCGATTCA GCCCTGGCTG GAGTGCCACC ACCTCACCCA 
GTCCAGAGCT ACCCACAGCA GAGCCATCAC CTGGCAGCCT CCATGAGCCA GGAGGGCTAC 
CACCAGGTCC CCAGCCTTCT GCCTGCCCGC CAGCCTGGCT TCATGGAGCC CCAAACAGGC 
CCGATGGGGG TGGCTACAGC AGGCTTTGGC CTAGTGCAGC CCCGGCCTCC CCTCGAGCCC 
AGCCCCACTG GCCGCCACCG TGGGGTACGT GCTGTGCAGC AGCAGCTGGC CTACGCCAGG 
GCCACAGGCC ATGCCATGGC TGCCATGCCG TCCAGTCAGG AAACAGCAGA GGCTGTGCCC 
AAGGGAGCGA TGGGCAACAT GGGGTCGGTG CCTCCCCAGC CGCCTCCGCA GGACGCAGGT 
GGGGCCCCGG ACCACAGCAT GCTCTACTAC TACGGCCAGA TCCACATGTA CGAACAGGAT 
GGAGGCCTGG AGAACCTCGG GAGCTGCCAG GTCATGCGGT CCCAGCCACC ACAGCCACAG 
GCCTGTCAGG ACAGCATCCA GCCCCAGCCC TTGCCCTCAC CAGGGGTCAA CCAGGTGTCC 
AGCACTGTGG ACTCCCAGCT CCTGGAGGCC CCCCAGATTG ACTTCGATGC CATCATGGAT 
GATGGCGATC ACTCGAGTTT GTTCTCGGGT GCTCTGAGCC CCAGCCTCCT CCACAGCCTC 
TCCCAGAACT CCTCCCGCCT CACCACCCCC CGAAACTCCT TGACCCTGCC CTCCATCCCC 
GCAGGCATCA GCAACATGGC TGTCGGGGAC ATGAGCTCCA TGCTCACCAG CCTCGCCGAG 
GAGAGCAAGT TCCTGAACAT GATGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( XM_006712422.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712485.1 Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X5, mRNA.

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CloneID	OHu36103
Clone ID Related Accession (Same CDS sequence)	NM_001374353.1 , XM_006712422.2 , XM_006712422.3
Accession Version	XM_006712422.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	zinc finger protein GLI2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006712422.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG 
GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG 
GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG 
CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC 
CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG 
ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG 
TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC 
ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG 
AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG 
ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT 
GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC 
CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC 
GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC 
AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC 
CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG 
AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA 
CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG 
CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG 
GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG 
CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGAGCAGCT GGCTGACCTC 
AAGGAAGATC TGGACAGGGA TGACTGTAAG CAGGAGGCTG AGGTGGTCAT CTATGAGACC 
AACTGCCACT GGGAAGACTG CACCAAGGAG TACGACACCC AGGAGCAGCT GGTGCATCAC 
ATCAACAACG AGCACATCCA CGGGGAGAAG AAGGAGTTTG TGTGCCGCTG GCAGGCCTGC 
ACGCGGGAGC AGAAGCCCTT CAAGGCGCAG TACATGCTGG TGGTGCACAT GCGGCGACAC 
ACGGGCGAGA AGCCCCACAA GTGCACGTTC GAGGGCTGCT CGAAGGCCTA CTCCCGCCTG 
GAGAACCTGA AGACACACCT GCGGTCCCAC ACCGGGGAGA AGCCATATGT GTGTGAGCAC 
GAGGGCTGCA ACAAAGCCTT CTCCAACGCC TCGGACCGCG CCAAGCACCA GAATCGCACC 
CACTCCAACG AGAAACCCTA CATCTGCAAG ATCCCAGGCT GCACCAAGAG ATACACAGAC 
CCCAGCTCTC TCCGGAAGCA TGTGAAAACG GTCCACGGCC CAGATGCCCA CGTCACCAAG 
AAGCAGCGCA ATGACGTGCA CCTCCGCACA CCGCTGCTCA AAGAGAATGG GGACAGTGAG 
GCCGGCACGG AGCCTGGCGG CCCAGAGAGC ACCGAGGCCA GCAGCACCAG CCAGGCCGTG 
GAGGACTGCC TGCACGTCAG AGCCATCAAG ACCGAGAGCT CCGGGCTGTG TCAGTCCAGC 
CCCGGGGCCC AGTCGTCCTG CAGCAGCGAG CCCTCTCCTC TGGGCAGTGC CCCCAACAAT 
GACAGTGGCG TGGAGATGCC GGGGACGGGG CCCGGGAGCC TGGGAGACCT GACGGCACTG 
GATGACACAC CCCCAGGGGC CGACACCTCA GCCCTGGCTG CCCCCTCCGC TGGTGGCCTC 
CAGCTGCGCA AACACATGAC CACCATGCAC CGGTTCGAGC AGCTCAAGAA GGAGAAGCTC 
AAGTCACTCA AGGATTCCTG CTCATGGGCC GGGCCGACTC CACACACGCG GAACACCAAG 
CTGCCTCCCC TCCCGGGAAG TGGCTCCATC CTGGAAAACT TCAGTGGCAG TGGGGGCGGC 
GGGCCCGCGG GGCTGCTGCC GAACCCGCGG CTGTCGGAGC TGTCCGCGAG CGAGGTGACC 
ATGCTGAGCC AGCTGCAGGA GCGCCGCGAC AGCTCCACCA GCACGGTCAG CTCGGCCTAC 
ACCGTGAGCC GCCGCTCCTC CGGCATCTCC CCCTACTTCT CCAGCCGCCG CTCCAGCGAG 
GCCTCGCCCC TGGGCGCCGG CCGCCCGCAC AACGCGAGCT CCGCTGACTC CTACGACCCC 
ATCTCCACGG ACGCGTCGCG GCGCTCGAGC GAGGCCAGCC AGTGCAGCGG CGGCTCCGGG 
CTGCTCAACC TCACGCCGGC GCAGCAGTAC AGCCTGCGGG CCAAGTACGC GGCAGCCACT 
GGCGGCCCCC CGCCCACTCC GCTGCCGGGC CTGGAGCGCA TGAGCCTGCG GACCAGGCTG 
GCGCTGCTGG ACGCGCCCGA GCGCACGCTG CCCGCCGGCT GCCCACGCCC ACTGGGGCCG 
CGGCGTGGCA GCGACGGGCC GACCTATGGC CACGGCCACG CGGGGGCTGC GCCCGCCTTC 
CCCCACGAGG CTCCAGGCGG CGGAGCCAGG CGGGCCAGCG ACCCTGTGCG GCGGCCCGAT 
GCCCTGTCCC TGCCGCGGGT GCAGCGCTTC CACAGCACCC ACAACGTGAA CCCCGGCCCG 
CTGCCGCCCT GTGCCGACAG GCGAGGCCTC CGCCTGCAGA GCCACCCGAG CACCGACGGC 
GGCCTGGCCC GCGGCGCCTA CTCGCCCCGG CCGCCTAGCA TCAGCGAGAA CGTGGCGATG 
GAGGCCGTGG CGGCAGGAGT GGACGGCGCG GGGCCCGAGG CCGACCTGGG GCTGCCGGAG 
GACGACCTGG TGCTTCCAGA CGACGTGGTG CAGTACATCA AGGCGCACGC CAGTGGCGCT 
CTGGACGAGG GCACCGGGCA GGTGTATCCC ACGGAAAGCA CTGGCTTCTC TGACAACCCC 
AGACTACCCA GCCCGGGGCT GCACGGCCAG CGCAGGATGG TGGCTGCGGA CTCCAACGTG 
GGCCCCTCCG CCCCTATGCT GGGAGGATGC CAGTTAGGCT TTGGGGCGCC CTCCAGCCTG 
AACAAAAATA ACATGCCTGT GCAGTGGAAT GAGGTGAGCT CCGGCACCGT AGACGCCCTG 
GCCAGCCAGG TGAAGCCTCC ACCCTTTCCT CAGGGCAACC TGGCGGTGGT GCAGCAGAAG 
CCTGCCTTTG GCCAGTACCC GGGCTACAGT CCGCAAGGCC TACAGGCTAG CCCTGGGGGC 
CTGGACAGCA CGCAGCCACA CCTGCAGCCC CGCAGCGGAG CCCCCTCCCA GGGCATCCCC 
AGGGTAAACT ACATGCAGCA GCTGCGACAG CCAGTGGCAG GCAGCCAGTG TCCTGGCATG 
ACTACCACTA TGAGCCCCCA TGCCTGCTAT GGCCAAGTCC ACCCCCAGCT GAGCCCCAGC 
ACCATCAGTG GGGCCCTCAA CCAGTTCCCC CAATCCTGCA GCAACATGCC AGCCAAGCCA 
GGGCATCTGG GGCACCCTCA GCAGACAGAA GTGGCACCTG ACCCCACCAC GATGGGCAAT 
CGCCACAGGG AACTTGGGGT CCCCGATTCA GCCCTGGCTG GAGTGCCACC ACCTCACCCA 
GTCCAGAGCT ACCCACAGCA GAGCCATCAC CTGGCAGCCT CCATGAGCCA GGAGGGCTAC 
CACCAGGTCC CCAGCCTTCT GCCTGCCCGC CAGCCTGGCT TCATGGAGCC CCAAACAGGC 
CCGATGGGGG TGGCTACAGC AGGCTTTGGC CTAGTGCAGC CCCGGCCTCC CCTCGAGCCC 
AGCCCCACTG GCCGCCACCG TGGGGTACGT GCTGTGCAGC AGCAGCTGGC CTACGCCAGG 
GCCACAGGCC ATGCCATGGC TGCCATGCCG TCCAGTCAGG AAACAGCAGA GGCTGTGCCC 
AAGGGAGCGA TGGGCAACAT GGGGTCGGTG CCTCCCCAGC CGCCTCCGCA GGACGCAGGT 
GGGGCCCCGG ACCACAGCAT GCTCTACTAC TACGGCCAGA TCCACATGTA CGAACAGGAT 
GGAGGCCTGG AGAACCTCGG GAGCTGCCAG GTCATGCGGT CCCAGCCACC ACAGCCACAG 
GCCTGTCAGG ACAGCATCCA GCCCCAGCCC TTGCCCTCAC CAGGGGTCAA CCAGGTGTCC 
AGCACTGTGG ACTCCCAGCT CCTGGAGGCC CCCCAGATTG ACTTCGATGC CATCATGGAT 
GATGGCGATC ACTCGAGTTT GTTCTCGGGT GCTCTGAGCC CCAGCCTCCT CCACAGCCTC 
TCCCAGAACT CCTCCCGCCT CACCACCCCC CGAAACTCCT TGACCCTGCC CTCCATCCCC 
GCAGGCATCA GCAACATGGC TGTCGGGGAC ATGAGCTCCA TGCTCACCAG CCTCGCCGAG 
GAGAGCAAGT TCCTGAACAT GATGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712485.1
CDS	452..5161
Translation	METSASATASEKQEAKSGILEAAGFPDPGKKASPLVVAAAAAAAVAAQGVPQHLLPPFHAPLPIDMRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	XM_006712422.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712422.3

ORF Insert Sequence:

ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG 
GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG 
GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG 
CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC 
CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG 
ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG 
TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC 
ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG 
AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG 
ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT 
GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC 
CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC 
GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC 
AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC 
CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG 
AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA 
CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG 
CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG 
GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG 
CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGAGCAGCT GGCTGACCTC 
AAGGAAGATC TGGACAGGGA TGACTGTAAG CAGGAGGCTG AGGTGGTCAT CTATGAGACC 
AACTGCCACT GGGAAGACTG CACCAAGGAG TACGACACCC AGGAGCAGCT GGTGCATCAC 
ATCAACAACG AGCACATCCA CGGGGAGAAG AAGGAGTTTG TGTGCCGCTG GCAGGCCTGC 
ACGCGGGAGC AGAAGCCCTT CAAGGCGCAG TACATGCTGG TGGTGCACAT GCGGCGACAC 
ACGGGCGAGA AGCCCCACAA GTGCACGTTC GAGGGCTGCT CGAAGGCCTA CTCCCGCCTG 
GAGAACCTGA AGACACACCT GCGGTCCCAC ACCGGGGAGA AGCCATATGT GTGTGAGCAC 
GAGGGCTGCA ACAAAGCCTT CTCCAACGCC TCGGACCGCG CCAAGCACCA GAATCGCACC 
CACTCCAACG AGAAACCCTA CATCTGCAAG ATCCCAGGCT GCACCAAGAG ATACACAGAC 
CCCAGCTCTC TCCGGAAGCA TGTGAAAACG GTCCACGGCC CAGATGCCCA CGTCACCAAG 
AAGCAGCGCA ATGACGTGCA CCTCCGCACA CCGCTGCTCA AAGAGAATGG GGACAGTGAG 
GCCGGCACGG AGCCTGGCGG CCCAGAGAGC ACCGAGGCCA GCAGCACCAG CCAGGCCGTG 
GAGGACTGCC TGCACGTCAG AGCCATCAAG ACCGAGAGCT CCGGGCTGTG TCAGTCCAGC 
CCCGGGGCCC AGTCGTCCTG CAGCAGCGAG CCCTCTCCTC TGGGCAGTGC CCCCAACAAT 
GACAGTGGCG TGGAGATGCC GGGGACGGGG CCCGGGAGCC TGGGAGACCT GACGGCACTG 
GATGACACAC CCCCAGGGGC CGACACCTCA GCCCTGGCTG CCCCCTCCGC TGGTGGCCTC 
CAGCTGCGCA AACACATGAC CACCATGCAC CGGTTCGAGC AGCTCAAGAA GGAGAAGCTC 
AAGTCACTCA AGGATTCCTG CTCATGGGCC GGGCCGACTC CACACACGCG GAACACCAAG 
CTGCCTCCCC TCCCGGGAAG TGGCTCCATC CTGGAAAACT TCAGTGGCAG TGGGGGCGGC 
GGGCCCGCGG GGCTGCTGCC GAACCCGCGG CTGTCGGAGC TGTCCGCGAG CGAGGTGACC 
ATGCTGAGCC AGCTGCAGGA GCGCCGCGAC AGCTCCACCA GCACGGTCAG CTCGGCCTAC 
ACCGTGAGCC GCCGCTCCTC CGGCATCTCC CCCTACTTCT CCAGCCGCCG CTCCAGCGAG 
GCCTCGCCCC TGGGCGCCGG CCGCCCGCAC AACGCGAGCT CCGCTGACTC CTACGACCCC 
ATCTCCACGG ACGCGTCGCG GCGCTCGAGC GAGGCCAGCC AGTGCAGCGG CGGCTCCGGG 
CTGCTCAACC TCACGCCGGC GCAGCAGTAC AGCCTGCGGG CCAAGTACGC GGCAGCCACT 
GGCGGCCCCC CGCCCACTCC GCTGCCGGGC CTGGAGCGCA TGAGCCTGCG GACCAGGCTG 
GCGCTGCTGG ACGCGCCCGA GCGCACGCTG CCCGCCGGCT GCCCACGCCC ACTGGGGCCG 
CGGCGTGGCA GCGACGGGCC GACCTATGGC CACGGCCACG CGGGGGCTGC GCCCGCCTTC 
CCCCACGAGG CTCCAGGCGG CGGAGCCAGG CGGGCCAGCG ACCCTGTGCG GCGGCCCGAT 
GCCCTGTCCC TGCCGCGGGT GCAGCGCTTC CACAGCACCC ACAACGTGAA CCCCGGCCCG 
CTGCCGCCCT GTGCCGACAG GCGAGGCCTC CGCCTGCAGA GCCACCCGAG CACCGACGGC 
GGCCTGGCCC GCGGCGCCTA CTCGCCCCGG CCGCCTAGCA TCAGCGAGAA CGTGGCGATG 
GAGGCCGTGG CGGCAGGAGT GGACGGCGCG GGGCCCGAGG CCGACCTGGG GCTGCCGGAG 
GACGACCTGG TGCTTCCAGA CGACGTGGTG CAGTACATCA AGGCGCACGC CAGTGGCGCT 
CTGGACGAGG GCACCGGGCA GGTGTATCCC ACGGAAAGCA CTGGCTTCTC TGACAACCCC 
AGACTACCCA GCCCGGGGCT GCACGGCCAG CGCAGGATGG TGGCTGCGGA CTCCAACGTG 
GGCCCCTCCG CCCCTATGCT GGGAGGATGC CAGTTAGGCT TTGGGGCGCC CTCCAGCCTG 
AACAAAAATA ACATGCCTGT GCAGTGGAAT GAGGTGAGCT CCGGCACCGT AGACGCCCTG 
GCCAGCCAGG TGAAGCCTCC ACCCTTTCCT CAGGGCAACC TGGCGGTGGT GCAGCAGAAG 
CCTGCCTTTG GCCAGTACCC GGGCTACAGT CCGCAAGGCC TACAGGCTAG CCCTGGGGGC 
CTGGACAGCA CGCAGCCACA CCTGCAGCCC CGCAGCGGAG CCCCCTCCCA GGGCATCCCC 
AGGGTAAACT ACATGCAGCA GCTGCGACAG CCAGTGGCAG GCAGCCAGTG TCCTGGCATG 
ACTACCACTA TGAGCCCCCA TGCCTGCTAT GGCCAAGTCC ACCCCCAGCT GAGCCCCAGC 
ACCATCAGTG GGGCCCTCAA CCAGTTCCCC CAATCCTGCA GCAACATGCC AGCCAAGCCA 
GGGCATCTGG GGCACCCTCA GCAGACAGAA GTGGCACCTG ACCCCACCAC GATGGGCAAT 
CGCCACAGGG AACTTGGGGT CCCCGATTCA GCCCTGGCTG GAGTGCCACC ACCTCACCCA 
GTCCAGAGCT ACCCACAGCA GAGCCATCAC CTGGCAGCCT CCATGAGCCA GGAGGGCTAC 
CACCAGGTCC CCAGCCTTCT GCCTGCCCGC CAGCCTGGCT TCATGGAGCC CCAAACAGGC 
CCGATGGGGG TGGCTACAGC AGGCTTTGGC CTAGTGCAGC CCCGGCCTCC CCTCGAGCCC 
AGCCCCACTG GCCGCCACCG TGGGGTACGT GCTGTGCAGC AGCAGCTGGC CTACGCCAGG 
GCCACAGGCC ATGCCATGGC TGCCATGCCG TCCAGTCAGG AAACAGCAGA GGCTGTGCCC 
AAGGGAGCGA TGGGCAACAT GGGGTCGGTG CCTCCCCAGC CGCCTCCGCA GGACGCAGGT 
GGGGCCCCGG ACCACAGCAT GCTCTACTAC TACGGCCAGA TCCACATGTA CGAACAGGAT 
GGAGGCCTGG AGAACCTCGG GAGCTGCCAG GTCATGCGGT CCCAGCCACC ACAGCCACAG 
GCCTGTCAGG ACAGCATCCA GCCCCAGCCC TTGCCCTCAC CAGGGGTCAA CCAGGTGTCC 
AGCACTGTGG ACTCCCAGCT CCTGGAGGCC CCCCAGATTG ACTTCGATGC CATCATGGAT 
GATGGCGATC ACTCGAGTTT GTTCTCGGGT GCTCTGAGCC CCAGCCTCCT CCACAGCCTC 
TCCCAGAACT CCTCCCGCCT CACCACCCCC CGAAACTCCT TGACCCTGCC CTCCATCCCC 
GCAGGCATCA GCAACATGGC TGTCGGGGAC ATGAGCTCCA TGCTCACCAG CCTCGCCGAG 
GAGAGCAAGT TCCTGAACAT GATGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( XM_011510970.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509272.1 Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X7, mRNA.

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CloneID	OHu57983
Clone ID Related Accession (Same CDS sequence)	XM_011510970.2
Accession Version	XM_011510970.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	zinc finger protein GLI2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510970.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTGC CGCAGCATCT CTTGCCACCA TTCCATGCGC CCCTACCGAT TGACATGCGA 
CACCAGGAAG GAAGGTACCA TTACGAGCCT CATTCTGTCC ACGGTGTGCA CGGGCCCCCT 
GCCCTCAGCG GCAGCCCTGT CATCTCTGAC ATCTCCTTGA TCCGGCTTTC CCCGCACCCG 
GCTGGCCCTG GGGAGTCCCC CTTCAACGCC CCCCACCCGT ACGTGAACCC CCACATGGAG 
CACTACCTCC GTTCTGTGCA CAGCAGCCCC ACGCTCTCCA TGATCTCTGC AGCCAGGGGC 
CTCAGCCCCG CTGATGTGGC CCAGGAGCAC CTTAAGGAGA GGGGACTGTT TGGCCTTCCT 
GCTCCAGGCA CCACCCCCTC AGACTATTAC CACCAGATGA CCCTCGTGGC AGGCCACCCC 
GCGCCCTACG GGGACCTGCT GATGCAGAGC GGGGGCGCTG CCAGCGCACC CCATCTCCAC 
GACTACCTCA ACCCCGTGGA CGTGTCCCGT TTCTCCAGCC CGCGGGTGAC GCCCCGCCTG 
AGCCGCAAGC GGGCGCTGTC CATCTCCCCA CTCTCAGACG CCAGCCTGGA CCTGCAGCGG 
ATGATCCGCA CCTCACCCAA CTCGCTAGTG GCCTACATCA ACAACTCCCG AAGCAGCTCG 
GCGGCCAGCG GTTCCTACGG GCATCTGTCA GCGGGTGCCC TCAGCCCAGC CTTCACCTTC 
CCCCACCCCA TCAACCCCGT GGCCTACCAG CAGATTCTGA GCCAGCAGAG GGGTCTGGGG 
TCAGCCTTTG GACACACACC ACCCCTGATC CAGCCCTCAC CCACCTTCCT GGCCCAGCAG 
CCCATGGCCC TCACCTCCAT CAATGCCACG CCCACCCAGC TCAGCAGCAG CAGCAACTGT 
CTGAGTGACA CCAACCAGAA CAAGCAGAGC AGTGAGTCGG CCGTCAGCAG CACCGTCAAC 
CCTGTCGCCA TTCACAAGCG CAGCAAGGTC AAGACCGAGC CTGAGGGCCT GCGGCCGGCC 
TCCCCTCTGG CGCTGACGCA GGGCCAGGTG TCTGGACACG GCTCATGTGG GTGTGCCCTT 
CCCCTCTCCC AGGAGCAGCT GGCTGACCTC AAGGAAGATC TGGACAGGGA TGACTGTAAG 
CAGGAGGCTG AGGTGGTCAT CTATGAGACC AACTGCCACT GGGAAGACTG CACCAAGGAG 
TACGACACCC AGGAGCAGCT GGTGCATCAC ATCAACAACG AGCACATCCA CGGGGAGAAG 
AAGGAGTTTG TGTGCCGCTG GCAGGCCTGC ACGCGGGAGC AGAAGCCCTT CAAGGCGCAG 
TACATGCTGG TGGTGCACAT GCGGCGACAC ACGGGCGAGA AGCCCCACAA GTGCACGTTC 
GAGGGCTGCT CGAAGGCCTA CTCCCGCCTG GAGAACCTGA AGACACACCT GCGGTCCCAC 
ACCGGGGAGA AGCCATATGT GTGTGAGCAC GAGGGCTGCA ACAAAGCCTT CTCCAACGCC 
TCGGACCGCG CCAAGCACCA GAATCGCACC CACTCCAACG AGAAACCCTA CATCTGCAAG 
ATCCCAGGCT GCACCAAGAG ATACACAGAC CCCAGCTCTC TCCGGAAGCA TGTGAAAACG 
GTCCACGGCC CAGATGCCCA CGTCACCAAG AAGCAGCGCA ATGACGTGCA CCTCCGCACA 
CCGCTGCTCA AAGAGAATGG GGACAGTGAG GCCGGCACGG AGCCTGGCGG CCCAGAGAGC 
ACCGAGGCCA GCAGCACCAG CCAGGCCGTG GAGGACTGCC TGCACGTCAG AGCCATCAAG 
ACCGAGAGCT CCGGGCTGTG TCAGTCCAGC CCCGGGGCCC AGTCGTCCTG CAGCAGCGAG 
CCCTCTCCTC TGGGCAGTGC CCCCAACAAT GACAGTGGCG TGGAGATGCC GGGGACGGGG 
CCCGGGAGCC TGGGAGACCT GACGGCACTG GATGACACAC CCCCAGGGGC CGACACCTCA 
GCCCTGGCTG CCCCCTCCGC TGGTGGCCTC CAGCTGCGCA AACACATGAC CACCATGCAC 
CGGTTCGAGC AGCTCAAGAA GGAGAAGCTC AAGTCACTCA AGGATTCCTG CTCATGGGCC 
GGGCCGACTC CACACACGCG GAACACCAAG CTGCCTCCCC TCCCGGGAAG TGGCTCCATC 
CTGGAAAACT TCAGTGGCAG TGGGGGCGGC GGGCCCGCGG GGCTGCTGCC GAACCCGCGG 
CTGTCGGAGC TGTCCGCGAG CGAGGTGACC ATGCTGAGCC AGCTGCAGGA GCGCCGCGAC 
AGCTCCACCA GCACGGTCAG CTCGGCCTAC ACCGTGAGCC GCCGCTCCTC CGGCATCTCC 
CCCTACTTCT CCAGCCGCCG CTCCAGCGAG GCCTCGCCCC TGGGCGCCGG CCGCCCGCAC 
AACGCGAGCT CCGCTGACTC CTACGACCCC ATCTCCACGG ACGCGTCGCG GCGCTCGAGC 
GAGGCCAGCC AGTGCAGCGG CGGCTCCGGG CTGCTCAACC TCACGCCGGC GCAGCAGTAC 
AGCCTGCGGG CCAAGTACGC GGCAGCCACT GGCGGCCCCC CGCCCACTCC GCTGCCGGGC 
CTGGAGCGCA TGAGCCTGCG GACCAGGCTG GCGCTGCTGG ACGCGCCCGA GCGCACGCTG 
CCCGCCGGCT GCCCACGCCC ACTGGGGCCG CGGCGTGGCA GCGACGGGCC GACCTATGGC 
CACGGCCACG CGGGGGCTGC GCCCGCCTTC CCCCACGAGG CTCCAGGCGG CGGAGCCAGG 
CGGGCCAGCG ACCCTGTGCG GCGGCCCGAT GCCCTGTCCC TGCCGCGGGT GCAGCGCTTC 
CACAGCACCC ACAACGTGAA CCCCGGCCCG CTGCCGCCCT GTGCCGACAG GCGAGGCCTC 
CGCCTGCAGA GCCACCCGAG CACCGACGGC GGCCTGGCCC GCGGCGCCTA CTCGCCCCGG 
CCGCCTAGCA TCAGCGAGAA CGTGGCGATG GAGGCCGTGG CGGCAGGAGT GGACGGCGCG 
GGGCCCGAGG CCGACCTGGG GCTGCCGGAG GACGACCTGG TGCTTCCAGA CGACGTGGTG 
CAGTACATCA AGGCGCACGC CAGTGGCGCT CTGGACGAGG GCACCGGGCA GGTGTATCCC 
ACGGAAAGCA CTGGCTTCTC TGACAACCCC AGACTACCCA GCCCGGGGCT GCACGGCCAG 
CGCAGGATGG TGGCTGCGGA CTCCAACGTG GGCCCCTCCG CCCCTATGCT GGGAGGATGC 
CAGTTAGGCT TTGGGGCGCC CTCCAGCCTG AACAAAAATA ACATGCCTGT GCAGTGGAAT 
GAGGTGAGCT CCGGCACCGT AGACGCCCTG GCCAGCCAGG TGAAGCCTCC ACCCTTTCCT 
CAGGGCAACC TGGCGGTGGT GCAGCAGAAG CCTGCCTTTG GCCAGTACCC GGGCTACAGT 
CCGCAAGGCC TACAGGCTAG CCCTGGGGGC CTGGACAGCA CGCAGCCACA CCTGCAGCCC 
CGCAGCGGAG CCCCCTCCCA GGGCATCCCC AGGGTAAACT ACATGCAGCA GCTGCGACAG 
CCAGTGGCAG GCAGCCAGTG TCCTGGCATG ACTACCACTA TGAGCCCCCA TGCCTGCTAT 
GGCCAAGTCC ACCCCCAGCT GAGCCCCAGC ACCATCAGTG GGGCCCTCAA CCAGTTCCCC 
CAATCCTGCA GCAACATGCC AGCCAAGCCA GGGCATCTGG GGCACCCTCA GCAGACAGAA 
GTGGCACCTG ACCCCACCAC GATGGGCAAT CGCCACAGGG AACTTGGGGT CCCCGATTCA 
GCCCTGGCTG GAGTGCCACC ACCTCACCCA GTCCAGAGCT ACCCACAGCA GAGCCATCAC 
CTGGCAGCCT CCATGAGCCA GGAGGGCTAC CACCAGGTCC CCAGCCTTCT GCCTGCCCGC 
CAGCCTGGCT TCATGGAGCC CCAAACAGGC CCGATGGGGG TGGCTACAGC AGGCTTTGGC 
CTAGTGCAGC CCCGGCCTCC CCTCGAGCCC AGCCCCACTG GCCGCCACCG TGGGGTACGT 
GCTGTGCAGC AGCAGCTGGC CTACGCCAGG GCCACAGGCC ATGCCATGGC TGCCATGCCG 
TCCAGTCAGG AAACAGCAGA GGCTGTGCCC AAGGGAGCGA TGGGCAACAT GGGGTCGGTG 
CCTCCCCAGC CGCCTCCGCA GGACGCAGGT GGGGCCCCGG ACCACAGCAT GCTCTACTAC 
TACGGCCAGA TCCACATGTA CGAACAGGAT GGAGGCCTGG AGAACCTCGG GAGCTGCCAG 
GTCATGCGGT CCCAGCCACC ACAGCCACAG GCCTGTCAGG ACAGCATCCA GCCCCAGCCC 
TTGCCCTCAC CAGGGGTCAA CCAGGTGTCC AGCACTGTGG ACTCCCAGCT CCTGGAGGCC 
CCCCAGATTG ACTTCGATGC CATCATGGAT GATGGCGATC ACTCGAGTTT GTTCTCGGGT 
GCTCTGAGCC CCAGCCTCCT CCACAGCCTC TCCCAGAACT CCTCCCGCCT CACCACCCCC 
CGAAACTCCT TGACCCTGCC CTCCATCCCC GCAGGCATCA GCAACATGGC TGTCGGGGAC 
ATGAGCTCCA TGCTCACCAG CCTCGCCGAG GAGAGCAAGT TCCTGAACAT GATGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509272.1
CDS	1815..6434
Translation	MLVPQHLLPPFHAPLPIDMRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQGQVSGHGSCGCALPLSQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	XM_011510970.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510970.2

ORF Insert Sequence:

ATGCTGGTGC CGCAGCATCT CTTGCCACCA TTCCATGCGC CCCTACCGAT TGACATGCGA 
CACCAGGAAG GAAGGTACCA TTACGAGCCT CATTCTGTCC ACGGTGTGCA CGGGCCCCCT 
GCCCTCAGCG GCAGCCCTGT CATCTCTGAC ATCTCCTTGA TCCGGCTTTC CCCGCACCCG 
GCTGGCCCTG GGGAGTCCCC CTTCAACGCC CCCCACCCGT ACGTGAACCC CCACATGGAG 
CACTACCTCC GTTCTGTGCA CAGCAGCCCC ACGCTCTCCA TGATCTCTGC AGCCAGGGGC 
CTCAGCCCCG CTGATGTGGC CCAGGAGCAC CTTAAGGAGA GGGGACTGTT TGGCCTTCCT 
GCTCCAGGCA CCACCCCCTC AGACTATTAC CACCAGATGA CCCTCGTGGC AGGCCACCCC 
GCGCCCTACG GGGACCTGCT GATGCAGAGC GGGGGCGCTG CCAGCGCACC CCATCTCCAC 
GACTACCTCA ACCCCGTGGA CGTGTCCCGT TTCTCCAGCC CGCGGGTGAC GCCCCGCCTG 
AGCCGCAAGC GGGCGCTGTC CATCTCCCCA CTCTCAGACG CCAGCCTGGA CCTGCAGCGG 
ATGATCCGCA CCTCACCCAA CTCGCTAGTG GCCTACATCA ACAACTCCCG AAGCAGCTCG 
GCGGCCAGCG GTTCCTACGG GCATCTGTCA GCGGGTGCCC TCAGCCCAGC CTTCACCTTC 
CCCCACCCCA TCAACCCCGT GGCCTACCAG CAGATTCTGA GCCAGCAGAG GGGTCTGGGG 
TCAGCCTTTG GACACACACC ACCCCTGATC CAGCCCTCAC CCACCTTCCT GGCCCAGCAG 
CCCATGGCCC TCACCTCCAT CAATGCCACG CCCACCCAGC TCAGCAGCAG CAGCAACTGT 
CTGAGTGACA CCAACCAGAA CAAGCAGAGC AGTGAGTCGG CCGTCAGCAG CACCGTCAAC 
CCTGTCGCCA TTCACAAGCG CAGCAAGGTC AAGACCGAGC CTGAGGGCCT GCGGCCGGCC 
TCCCCTCTGG CGCTGACGCA GGGCCAGGTG TCTGGACACG GCTCATGTGG GTGTGCCCTT 
CCCCTCTCCC AGGAGCAGCT GGCTGACCTC AAGGAAGATC TGGACAGGGA TGACTGTAAG 
CAGGAGGCTG AGGTGGTCAT CTATGAGACC AACTGCCACT GGGAAGACTG CACCAAGGAG 
TACGACACCC AGGAGCAGCT GGTGCATCAC ATCAACAACG AGCACATCCA CGGGGAGAAG 
AAGGAGTTTG TGTGCCGCTG GCAGGCCTGC ACGCGGGAGC AGAAGCCCTT CAAGGCGCAG 
TACATGCTGG TGGTGCACAT GCGGCGACAC ACGGGCGAGA AGCCCCACAA GTGCACGTTC 
GAGGGCTGCT CGAAGGCCTA CTCCCGCCTG GAGAACCTGA AGACACACCT GCGGTCCCAC 
ACCGGGGAGA AGCCATATGT GTGTGAGCAC GAGGGCTGCA ACAAAGCCTT CTCCAACGCC 
TCGGACCGCG CCAAGCACCA GAATCGCACC CACTCCAACG AGAAACCCTA CATCTGCAAG 
ATCCCAGGCT GCACCAAGAG ATACACAGAC CCCAGCTCTC TCCGGAAGCA TGTGAAAACG 
GTCCACGGCC CAGATGCCCA CGTCACCAAG AAGCAGCGCA ATGACGTGCA CCTCCGCACA 
CCGCTGCTCA AAGAGAATGG GGACAGTGAG GCCGGCACGG AGCCTGGCGG CCCAGAGAGC 
ACCGAGGCCA GCAGCACCAG CCAGGCCGTG GAGGACTGCC TGCACGTCAG AGCCATCAAG 
ACCGAGAGCT CCGGGCTGTG TCAGTCCAGC CCCGGGGCCC AGTCGTCCTG CAGCAGCGAG 
CCCTCTCCTC TGGGCAGTGC CCCCAACAAT GACAGTGGCG TGGAGATGCC GGGGACGGGG 
CCCGGGAGCC TGGGAGACCT GACGGCACTG GATGACACAC CCCCAGGGGC CGACACCTCA 
GCCCTGGCTG CCCCCTCCGC TGGTGGCCTC CAGCTGCGCA AACACATGAC CACCATGCAC 
CGGTTCGAGC AGCTCAAGAA GGAGAAGCTC AAGTCACTCA AGGATTCCTG CTCATGGGCC 
GGGCCGACTC CACACACGCG GAACACCAAG CTGCCTCCCC TCCCGGGAAG TGGCTCCATC 
CTGGAAAACT TCAGTGGCAG TGGGGGCGGC GGGCCCGCGG GGCTGCTGCC GAACCCGCGG 
CTGTCGGAGC TGTCCGCGAG CGAGGTGACC ATGCTGAGCC AGCTGCAGGA GCGCCGCGAC 
AGCTCCACCA GCACGGTCAG CTCGGCCTAC ACCGTGAGCC GCCGCTCCTC CGGCATCTCC 
CCCTACTTCT CCAGCCGCCG CTCCAGCGAG GCCTCGCCCC TGGGCGCCGG CCGCCCGCAC 
AACGCGAGCT CCGCTGACTC CTACGACCCC ATCTCCACGG ACGCGTCGCG GCGCTCGAGC 
GAGGCCAGCC AGTGCAGCGG CGGCTCCGGG CTGCTCAACC TCACGCCGGC GCAGCAGTAC 
AGCCTGCGGG CCAAGTACGC GGCAGCCACT GGCGGCCCCC CGCCCACTCC GCTGCCGGGC 
CTGGAGCGCA TGAGCCTGCG GACCAGGCTG GCGCTGCTGG ACGCGCCCGA GCGCACGCTG 
CCCGCCGGCT GCCCACGCCC ACTGGGGCCG CGGCGTGGCA GCGACGGGCC GACCTATGGC 
CACGGCCACG CGGGGGCTGC GCCCGCCTTC CCCCACGAGG CTCCAGGCGG CGGAGCCAGG 
CGGGCCAGCG ACCCTGTGCG GCGGCCCGAT GCCCTGTCCC TGCCGCGGGT GCAGCGCTTC 
CACAGCACCC ACAACGTGAA CCCCGGCCCG CTGCCGCCCT GTGCCGACAG GCGAGGCCTC 
CGCCTGCAGA GCCACCCGAG CACCGACGGC GGCCTGGCCC GCGGCGCCTA CTCGCCCCGG 
CCGCCTAGCA TCAGCGAGAA CGTGGCGATG GAGGCCGTGG CGGCAGGAGT GGACGGCGCG 
GGGCCCGAGG CCGACCTGGG GCTGCCGGAG GACGACCTGG TGCTTCCAGA CGACGTGGTG 
CAGTACATCA AGGCGCACGC CAGTGGCGCT CTGGACGAGG GCACCGGGCA GGTGTATCCC 
ACGGAAAGCA CTGGCTTCTC TGACAACCCC AGACTACCCA GCCCGGGGCT GCACGGCCAG 
CGCAGGATGG TGGCTGCGGA CTCCAACGTG GGCCCCTCCG CCCCTATGCT GGGAGGATGC 
CAGTTAGGCT TTGGGGCGCC CTCCAGCCTG AACAAAAATA ACATGCCTGT GCAGTGGAAT 
GAGGTGAGCT CCGGCACCGT AGACGCCCTG GCCAGCCAGG TGAAGCCTCC ACCCTTTCCT 
CAGGGCAACC TGGCGGTGGT GCAGCAGAAG CCTGCCTTTG GCCAGTACCC GGGCTACAGT 
CCGCAAGGCC TACAGGCTAG CCCTGGGGGC CTGGACAGCA CGCAGCCACA CCTGCAGCCC 
CGCAGCGGAG CCCCCTCCCA GGGCATCCCC AGGGTAAACT ACATGCAGCA GCTGCGACAG 
CCAGTGGCAG GCAGCCAGTG TCCTGGCATG ACTACCACTA TGAGCCCCCA TGCCTGCTAT 
GGCCAAGTCC ACCCCCAGCT GAGCCCCAGC ACCATCAGTG GGGCCCTCAA CCAGTTCCCC 
CAATCCTGCA GCAACATGCC AGCCAAGCCA GGGCATCTGG GGCACCCTCA GCAGACAGAA 
GTGGCACCTG ACCCCACCAC GATGGGCAAT CGCCACAGGG AACTTGGGGT CCCCGATTCA 
GCCCTGGCTG GAGTGCCACC ACCTCACCCA GTCCAGAGCT ACCCACAGCA GAGCCATCAC 
CTGGCAGCCT CCATGAGCCA GGAGGGCTAC CACCAGGTCC CCAGCCTTCT GCCTGCCCGC 
CAGCCTGGCT TCATGGAGCC CCAAACAGGC CCGATGGGGG TGGCTACAGC AGGCTTTGGC 
CTAGTGCAGC CCCGGCCTCC CCTCGAGCCC AGCCCCACTG GCCGCCACCG TGGGGTACGT 
GCTGTGCAGC AGCAGCTGGC CTACGCCAGG GCCACAGGCC ATGCCATGGC TGCCATGCCG 
TCCAGTCAGG AAACAGCAGA GGCTGTGCCC AAGGGAGCGA TGGGCAACAT GGGGTCGGTG 
CCTCCCCAGC CGCCTCCGCA GGACGCAGGT GGGGCCCCGG ACCACAGCAT GCTCTACTAC 
TACGGCCAGA TCCACATGTA CGAACAGGAT GGAGGCCTGG AGAACCTCGG GAGCTGCCAG 
GTCATGCGGT CCCAGCCACC ACAGCCACAG GCCTGTCAGG ACAGCATCCA GCCCCAGCCC 
TTGCCCTCAC CAGGGGTCAA CCAGGTGTCC AGCACTGTGG ACTCCCAGCT CCTGGAGGCC 
CCCCAGATTG ACTTCGATGC CATCATGGAT GATGGCGATC ACTCGAGTTT GTTCTCGGGT 
GCTCTGAGCC CCAGCCTCCT CCACAGCCTC TCCCAGAACT CCTCCCGCCT CACCACCCCC 
CGAAACTCCT TGACCCTGCC CTCCATCCCC GCAGGCATCA GCAACATGGC TGTCGGGGAC 
ATGAGCTCCA TGCTCACCAG CCTCGCCGAG GAGAGCAAGT TCCTGAACAT GATGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( XM_011510971.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509273.1 Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X8, mRNA.

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CloneID	OHu57984
Clone ID Related Accession (Same CDS sequence)	XM_011510971.2
Accession Version	XM_011510971.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	zinc finger protein GLI2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510971.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACACC AGGAAGGAAG GTACCATTAC GAGCCTCATT CTGTCCACGG TGTGCACGGG 
CCCCCTGCCC TCAGCGGCAG CCCTGTCATC TCTGACATCT CCTTGATCCG GCTTTCCCCG 
CACCCGGCTG GCCCTGGGGA GTCCCCCTTC AACGCCCCCC ACCCGTACGT GAACCCCCAC 
ATGGAGCACT ACCTCCGTTC TGTGCACAGC AGCCCCACGC TCTCCATGAT CTCTGCAGCC 
AGGGGCCTCA GCCCCGCTGA TGTGGCCCAG GAGCACCTTA AGGAGAGGGG ACTGTTTGGC 
CTTCCTGCTC CAGGCACCAC CCCCTCAGAC TATTACCACC AGATGACCCT CGTGGCAGGC 
CACCCCGCGC CCTACGGGGA CCTGCTGATG CAGAGCGGGG GCGCTGCCAG CGCACCCCAT 
CTCCACGACT ACCTCAACCC CGTGGACGTG TCCCGTTTCT CCAGCCCGCG GGTGACGCCC 
CGCCTGAGCC GCAAGCGGGC GCTGTCCATC TCCCCACTCT CAGACGCCAG CCTGGACCTG 
CAGCGGATGA TCCGCACCTC ACCCAACTCG CTAGTGGCCT ACATCAACAA CTCCCGAAGC 
AGCTCGGCGG CCAGCGGTTC CTACGGGCAT CTGTCAGCGG GTGCCCTCAG CCCAGCCTTC 
ACCTTCCCCC ACCCCATCAA CCCCGTGGCC TACCAGCAGA TTCTGAGCCA GCAGAGGGGT 
CTGGGGTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CCTCACCCAC CTTCCTGGCC 
CAGCAGCCCA TGGCCCTCAC CTCCATCAAT GCCACGCCCA CCCAGCTCAG CAGCAGCAGC 
AACTGTCTGA GTGACACCAA CCAGAACAAG CAGAGCAGTG AGTCGGCCGT CAGCAGCACC 
GTCAACCCTG TCGCCATTCA CAAGCGCAGC AAGGTCAAGA CCGAGCCTGA GGGCCTGCGG 
CCGGCCTCCC CTCTGGCGCT GACGCAGGGC CAGGTGTCTG GACACGGCTC ATGTGGGTGT 
GCCCTTCCCC TCTCCCAGGA GCAGCTGGCT GACCTCAAGG AAGATCTGGA CAGGGATGAC 
TGTAAGCAGG AGGCTGAGGT GGTCATCTAT GAGACCAACT GCCACTGGGA AGACTGCACC 
AAGGAGTACG ACACCCAGGA GCAGCTGGTG CATCACATCA ACAACGAGCA CATCCACGGG 
GAGAAGAAGG AGTTTGTGTG CCGCTGGCAG GCCTGCACGC GGGAGCAGAA GCCCTTCAAG 
GCGCAGTACA TGCTGGTGGT GCACATGCGG CGACACACGG GCGAGAAGCC CCACAAGTGC 
ACGTTCGAGG GCTGCTCGAA GGCCTACTCC CGCCTGGAGA ACCTGAAGAC ACACCTGCGG 
TCCCACACCG GGGAGAAGCC ATATGTGTGT GAGCACGAGG GCTGCAACAA AGCCTTCTCC 
AACGCCTCGG ACCGCGCCAA GCACCAGAAT CGCACCCACT CCAACGAGAA ACCCTACATC 
TGCAAGATCC CAGGCTGCAC CAAGAGATAC ACAGACCCCA GCTCTCTCCG GAAGCATGTG 
AAAACGGTCC ACGGCCCAGA TGCCCACGTC ACCAAGAAGC AGCGCAATGA CGTGCACCTC 
CGCACACCGC TGCTCAAAGA GAATGGGGAC AGTGAGGCCG GCACGGAGCC TGGCGGCCCA 
GAGAGCACCG AGGCCAGCAG CACCAGCCAG GCCGTGGAGG ACTGCCTGCA CGTCAGAGCC 
ATCAAGACCG AGAGCTCCGG GCTGTGTCAG TCCAGCCCCG GGGCCCAGTC GTCCTGCAGC 
AGCGAGCCCT CTCCTCTGGG CAGTGCCCCC AACAATGACA GTGGCGTGGA GATGCCGGGG 
ACGGGGCCCG GGAGCCTGGG AGACCTGACG GCACTGGATG ACACACCCCC AGGGGCCGAC 
ACCTCAGCCC TGGCTGCCCC CTCCGCTGGT GGCCTCCAGC TGCGCAAACA CATGACCACC 
ATGCACCGGT TCGAGCAGCT CAAGAAGGAG AAGCTCAAGT CACTCAAGGA TTCCTGCTCA 
TGGGCCGGGC CGACTCCACA CACGCGGAAC ACCAAGCTGC CTCCCCTCCC GGGAAGTGGC 
TCCATCCTGG AAAACTTCAG TGGCAGTGGG GGCGGCGGGC CCGCGGGGCT GCTGCCGAAC 
CCGCGGCTGT CGGAGCTGTC CGCGAGCGAG GTGACCATGC TGAGCCAGCT GCAGGAGCGC 
CGCGACAGCT CCACCAGCAC GGTCAGCTCG GCCTACACCG TGAGCCGCCG CTCCTCCGGC 
ATCTCCCCCT ACTTCTCCAG CCGCCGCTCC AGCGAGGCCT CGCCCCTGGG CGCCGGCCGC 
CCGCACAACG CGAGCTCCGC TGACTCCTAC GACCCCATCT CCACGGACGC GTCGCGGCGC 
TCGAGCGAGG CCAGCCAGTG CAGCGGCGGC TCCGGGCTGC TCAACCTCAC GCCGGCGCAG 
CAGTACAGCC TGCGGGCCAA GTACGCGGCA GCCACTGGCG GCCCCCCGCC CACTCCGCTG 
CCGGGCCTGG AGCGCATGAG CCTGCGGACC AGGCTGGCGC TGCTGGACGC GCCCGAGCGC 
ACGCTGCCCG CCGGCTGCCC ACGCCCACTG GGGCCGCGGC GTGGCAGCGA CGGGCCGACC 
TATGGCCACG GCCACGCGGG GGCTGCGCCC GCCTTCCCCC ACGAGGCTCC AGGCGGCGGA 
GCCAGGCGGG CCAGCGACCC TGTGCGGCGG CCCGATGCCC TGTCCCTGCC GCGGGTGCAG 
CGCTTCCACA GCACCCACAA CGTGAACCCC GGCCCGCTGC CGCCCTGTGC CGACAGGCGA 
GGCCTCCGCC TGCAGAGCCA CCCGAGCACC GACGGCGGCC TGGCCCGCGG CGCCTACTCG 
CCCCGGCCGC CTAGCATCAG CGAGAACGTG GCGATGGAGG CCGTGGCGGC AGGAGTGGAC 
GGCGCGGGGC CCGAGGCCGA CCTGGGGCTG CCGGAGGACG ACCTGGTGCT TCCAGACGAC 
GTGGTGCAGT ACATCAAGGC GCACGCCAGT GGCGCTCTGG ACGAGGGCAC CGGGCAGGTG 
TATCCCACGG AAAGCACTGG CTTCTCTGAC AACCCCAGAC TACCCAGCCC GGGGCTGCAC 
GGCCAGCGCA GGATGGTGGC TGCGGACTCC AACGTGGGCC CCTCCGCCCC TATGCTGGGA 
GGATGCCAGT TAGGCTTTGG GGCGCCCTCC AGCCTGAACA AAAATAACAT GCCTGTGCAG 
TGGAATGAGG TGAGCTCCGG CACCGTAGAC GCCCTGGCCA GCCAGGTGAA GCCTCCACCC 
TTTCCTCAGG GCAACCTGGC GGTGGTGCAG CAGAAGCCTG CCTTTGGCCA GTACCCGGGC 
TACAGTCCGC AAGGCCTACA GGCTAGCCCT GGGGGCCTGG ACAGCACGCA GCCACACCTG 
CAGCCCCGCA GCGGAGCCCC CTCCCAGGGC ATCCCCAGGG TAAACTACAT GCAGCAGCTG 
CGACAGCCAG TGGCAGGCAG CCAGTGTCCT GGCATGACTA CCACTATGAG CCCCCATGCC 
TGCTATGGCC AAGTCCACCC CCAGCTGAGC CCCAGCACCA TCAGTGGGGC CCTCAACCAG 
TTCCCCCAAT CCTGCAGCAA CATGCCAGCC AAGCCAGGGC ATCTGGGGCA CCCTCAGCAG 
ACAGAAGTGG CACCTGACCC CACCACGATG GGCAATCGCC ACAGGGAACT TGGGGTCCCC 
GATTCAGCCC TGGCTGGAGT GCCACCACCT CACCCAGTCC AGAGCTACCC ACAGCAGAGC 
CATCACCTGG CAGCCTCCAT GAGCCAGGAG GGCTACCACC AGGTCCCCAG CCTTCTGCCT 
GCCCGCCAGC CTGGCTTCAT GGAGCCCCAA ACAGGCCCGA TGGGGGTGGC TACAGCAGGC 
TTTGGCCTAG TGCAGCCCCG GCCTCCCCTC GAGCCCAGCC CCACTGGCCG CCACCGTGGG 
GTACGTGCTG TGCAGCAGCA GCTGGCCTAC GCCAGGGCCA CAGGCCATGC CATGGCTGCC 
ATGCCGTCCA GTCAGGAAAC AGCAGAGGCT GTGCCCAAGG GAGCGATGGG CAACATGGGG 
TCGGTGCCTC CCCAGCCGCC TCCGCAGGAC GCAGGTGGGG CCCCGGACCA CAGCATGCTC 
TACTACTACG GCCAGATCCA CATGTACGAA CAGGATGGAG GCCTGGAGAA CCTCGGGAGC 
TGCCAGGTCA TGCGGTCCCA GCCACCACAG CCACAGGCCT GTCAGGACAG CATCCAGCCC 
CAGCCCTTGC CCTCACCAGG GGTCAACCAG GTGTCCAGCA CTGTGGACTC CCAGCTCCTG 
GAGGCCCCCC AGATTGACTT CGATGCCATC ATGGATGATG GCGATCACTC GAGTTTGTTC 
TCGGGTGCTC TGAGCCCCAG CCTCCTCCAC AGCCTCTCCC AGAACTCCTC CCGCCTCACC 
ACCCCCCGAA ACTCCTTGAC CCTGCCCTCC ATCCCCGCAG GCATCAGCAA CATGGCTGTC 
GGGGACATGA GCTCCATGCT CACCAGCCTC GCCGAGGAGA GCAAGTTCCT GAACATGATG 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509273.1
CDS	1119..5684
Translation	MRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQGQVSGHGSCGCALPLSQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	XM_011510971.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510971.2

ORF Insert Sequence:

ATGCGACACC AGGAAGGAAG GTACCATTAC GAGCCTCATT CTGTCCACGG TGTGCACGGG 
CCCCCTGCCC TCAGCGGCAG CCCTGTCATC TCTGACATCT CCTTGATCCG GCTTTCCCCG 
CACCCGGCTG GCCCTGGGGA GTCCCCCTTC AACGCCCCCC ACCCGTACGT GAACCCCCAC 
ATGGAGCACT ACCTCCGTTC TGTGCACAGC AGCCCCACGC TCTCCATGAT CTCTGCAGCC 
AGGGGCCTCA GCCCCGCTGA TGTGGCCCAG GAGCACCTTA AGGAGAGGGG ACTGTTTGGC 
CTTCCTGCTC CAGGCACCAC CCCCTCAGAC TATTACCACC AGATGACCCT CGTGGCAGGC 
CACCCCGCGC CCTACGGGGA CCTGCTGATG CAGAGCGGGG GCGCTGCCAG CGCACCCCAT 
CTCCACGACT ACCTCAACCC CGTGGACGTG TCCCGTTTCT CCAGCCCGCG GGTGACGCCC 
CGCCTGAGCC GCAAGCGGGC GCTGTCCATC TCCCCACTCT CAGACGCCAG CCTGGACCTG 
CAGCGGATGA TCCGCACCTC ACCCAACTCG CTAGTGGCCT ACATCAACAA CTCCCGAAGC 
AGCTCGGCGG CCAGCGGTTC CTACGGGCAT CTGTCAGCGG GTGCCCTCAG CCCAGCCTTC 
ACCTTCCCCC ACCCCATCAA CCCCGTGGCC TACCAGCAGA TTCTGAGCCA GCAGAGGGGT 
CTGGGGTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CCTCACCCAC CTTCCTGGCC 
CAGCAGCCCA TGGCCCTCAC CTCCATCAAT GCCACGCCCA CCCAGCTCAG CAGCAGCAGC 
AACTGTCTGA GTGACACCAA CCAGAACAAG CAGAGCAGTG AGTCGGCCGT CAGCAGCACC 
GTCAACCCTG TCGCCATTCA CAAGCGCAGC AAGGTCAAGA CCGAGCCTGA GGGCCTGCGG 
CCGGCCTCCC CTCTGGCGCT GACGCAGGGC CAGGTGTCTG GACACGGCTC ATGTGGGTGT 
GCCCTTCCCC TCTCCCAGGA GCAGCTGGCT GACCTCAAGG AAGATCTGGA CAGGGATGAC 
TGTAAGCAGG AGGCTGAGGT GGTCATCTAT GAGACCAACT GCCACTGGGA AGACTGCACC 
AAGGAGTACG ACACCCAGGA GCAGCTGGTG CATCACATCA ACAACGAGCA CATCCACGGG 
GAGAAGAAGG AGTTTGTGTG CCGCTGGCAG GCCTGCACGC GGGAGCAGAA GCCCTTCAAG 
GCGCAGTACA TGCTGGTGGT GCACATGCGG CGACACACGG GCGAGAAGCC CCACAAGTGC 
ACGTTCGAGG GCTGCTCGAA GGCCTACTCC CGCCTGGAGA ACCTGAAGAC ACACCTGCGG 
TCCCACACCG GGGAGAAGCC ATATGTGTGT GAGCACGAGG GCTGCAACAA AGCCTTCTCC 
AACGCCTCGG ACCGCGCCAA GCACCAGAAT CGCACCCACT CCAACGAGAA ACCCTACATC 
TGCAAGATCC CAGGCTGCAC CAAGAGATAC ACAGACCCCA GCTCTCTCCG GAAGCATGTG 
AAAACGGTCC ACGGCCCAGA TGCCCACGTC ACCAAGAAGC AGCGCAATGA CGTGCACCTC 
CGCACACCGC TGCTCAAAGA GAATGGGGAC AGTGAGGCCG GCACGGAGCC TGGCGGCCCA 
GAGAGCACCG AGGCCAGCAG CACCAGCCAG GCCGTGGAGG ACTGCCTGCA CGTCAGAGCC 
ATCAAGACCG AGAGCTCCGG GCTGTGTCAG TCCAGCCCCG GGGCCCAGTC GTCCTGCAGC 
AGCGAGCCCT CTCCTCTGGG CAGTGCCCCC AACAATGACA GTGGCGTGGA GATGCCGGGG 
ACGGGGCCCG GGAGCCTGGG AGACCTGACG GCACTGGATG ACACACCCCC AGGGGCCGAC 
ACCTCAGCCC TGGCTGCCCC CTCCGCTGGT GGCCTCCAGC TGCGCAAACA CATGACCACC 
ATGCACCGGT TCGAGCAGCT CAAGAAGGAG AAGCTCAAGT CACTCAAGGA TTCCTGCTCA 
TGGGCCGGGC CGACTCCACA CACGCGGAAC ACCAAGCTGC CTCCCCTCCC GGGAAGTGGC 
TCCATCCTGG AAAACTTCAG TGGCAGTGGG GGCGGCGGGC CCGCGGGGCT GCTGCCGAAC 
CCGCGGCTGT CGGAGCTGTC CGCGAGCGAG GTGACCATGC TGAGCCAGCT GCAGGAGCGC 
CGCGACAGCT CCACCAGCAC GGTCAGCTCG GCCTACACCG TGAGCCGCCG CTCCTCCGGC 
ATCTCCCCCT ACTTCTCCAG CCGCCGCTCC AGCGAGGCCT CGCCCCTGGG CGCCGGCCGC 
CCGCACAACG CGAGCTCCGC TGACTCCTAC GACCCCATCT CCACGGACGC GTCGCGGCGC 
TCGAGCGAGG CCAGCCAGTG CAGCGGCGGC TCCGGGCTGC TCAACCTCAC GCCGGCGCAG 
CAGTACAGCC TGCGGGCCAA GTACGCGGCA GCCACTGGCG GCCCCCCGCC CACTCCGCTG 
CCGGGCCTGG AGCGCATGAG CCTGCGGACC AGGCTGGCGC TGCTGGACGC GCCCGAGCGC 
ACGCTGCCCG CCGGCTGCCC ACGCCCACTG GGGCCGCGGC GTGGCAGCGA CGGGCCGACC 
TATGGCCACG GCCACGCGGG GGCTGCGCCC GCCTTCCCCC ACGAGGCTCC AGGCGGCGGA 
GCCAGGCGGG CCAGCGACCC TGTGCGGCGG CCCGATGCCC TGTCCCTGCC GCGGGTGCAG 
CGCTTCCACA GCACCCACAA CGTGAACCCC GGCCCGCTGC CGCCCTGTGC CGACAGGCGA 
GGCCTCCGCC TGCAGAGCCA CCCGAGCACC GACGGCGGCC TGGCCCGCGG CGCCTACTCG 
CCCCGGCCGC CTAGCATCAG CGAGAACGTG GCGATGGAGG CCGTGGCGGC AGGAGTGGAC 
GGCGCGGGGC CCGAGGCCGA CCTGGGGCTG CCGGAGGACG ACCTGGTGCT TCCAGACGAC 
GTGGTGCAGT ACATCAAGGC GCACGCCAGT GGCGCTCTGG ACGAGGGCAC CGGGCAGGTG 
TATCCCACGG AAAGCACTGG CTTCTCTGAC AACCCCAGAC TACCCAGCCC GGGGCTGCAC 
GGCCAGCGCA GGATGGTGGC TGCGGACTCC AACGTGGGCC CCTCCGCCCC TATGCTGGGA 
GGATGCCAGT TAGGCTTTGG GGCGCCCTCC AGCCTGAACA AAAATAACAT GCCTGTGCAG 
TGGAATGAGG TGAGCTCCGG CACCGTAGAC GCCCTGGCCA GCCAGGTGAA GCCTCCACCC 
TTTCCTCAGG GCAACCTGGC GGTGGTGCAG CAGAAGCCTG CCTTTGGCCA GTACCCGGGC 
TACAGTCCGC AAGGCCTACA GGCTAGCCCT GGGGGCCTGG ACAGCACGCA GCCACACCTG 
CAGCCCCGCA GCGGAGCCCC CTCCCAGGGC ATCCCCAGGG TAAACTACAT GCAGCAGCTG 
CGACAGCCAG TGGCAGGCAG CCAGTGTCCT GGCATGACTA CCACTATGAG CCCCCATGCC 
TGCTATGGCC AAGTCCACCC CCAGCTGAGC CCCAGCACCA TCAGTGGGGC CCTCAACCAG 
TTCCCCCAAT CCTGCAGCAA CATGCCAGCC AAGCCAGGGC ATCTGGGGCA CCCTCAGCAG 
ACAGAAGTGG CACCTGACCC CACCACGATG GGCAATCGCC ACAGGGAACT TGGGGTCCCC 
GATTCAGCCC TGGCTGGAGT GCCACCACCT CACCCAGTCC AGAGCTACCC ACAGCAGAGC 
CATCACCTGG CAGCCTCCAT GAGCCAGGAG GGCTACCACC AGGTCCCCAG CCTTCTGCCT 
GCCCGCCAGC CTGGCTTCAT GGAGCCCCAA ACAGGCCCGA TGGGGGTGGC TACAGCAGGC 
TTTGGCCTAG TGCAGCCCCG GCCTCCCCTC GAGCCCAGCC CCACTGGCCG CCACCGTGGG 
GTACGTGCTG TGCAGCAGCA GCTGGCCTAC GCCAGGGCCA CAGGCCATGC CATGGCTGCC 
ATGCCGTCCA GTCAGGAAAC AGCAGAGGCT GTGCCCAAGG GAGCGATGGG CAACATGGGG 
TCGGTGCCTC CCCAGCCGCC TCCGCAGGAC GCAGGTGGGG CCCCGGACCA CAGCATGCTC 
TACTACTACG GCCAGATCCA CATGTACGAA CAGGATGGAG GCCTGGAGAA CCTCGGGAGC 
TGCCAGGTCA TGCGGTCCCA GCCACCACAG CCACAGGCCT GTCAGGACAG CATCCAGCCC 
CAGCCCTTGC CCTCACCAGG GGTCAACCAG GTGTCCAGCA CTGTGGACTC CCAGCTCCTG 
GAGGCCCCCC AGATTGACTT CGATGCCATC ATGGATGATG GCGATCACTC GAGTTTGTTC 
TCGGGTGCTC TGAGCCCCAG CCTCCTCCAC AGCCTCTCCC AGAACTCCTC CCGCCTCACC 
ACCCCCCGAA ACTCCTTGAC CCTGCCCTCC ATCCCCGCAG GCATCAGCAA CATGGCTGTC 
GGGGACATGA GCTCCATGCT CACCAGCCTC GCCGAGGAGA GCAAGTTCCT GAACATGATG 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( XM_011510973.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509275.1 Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu57985
Clone ID Related Accession (Same CDS sequence)	XM_011510973.2
Accession Version	XM_011510973.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	zinc finger protein GLI2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510973.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACT ACCTCCGTTC TGTGCACAGC AGCCCCACGC TCTCCATGAT CTCTGCAGCC 
AGGGGCCTCA GCCCCGCTGA TGTGGCCCAG GAGCACCTTA AGGAGAGGGG ACTGTTTGGC 
CTTCCTGCTC CAGGCACCAC CCCCTCAGAC TATTACCACC AGATGACCCT CGTGGCAGGC 
CACCCCGCGC CCTACGGGGA CCTGCTGATG CAGAGCGGGG GCGCTGCCAG CGCACCCCAT 
CTCCACGACT ACCTCAACCC CGTGGACGTG TCCCGTTTCT CCAGCCCGCG GGTGACGCCC 
CGCCTGAGCC GCAAGCGGGC GCTGTCCATC TCCCCACTCT CAGACGCCAG CCTGGACCTG 
CAGCGGATGA TCCGCACCTC ACCCAACTCG CTAGTGGCCT ACATCAACAA CTCCCGAAGC 
AGCTCGGCGG CCAGCGGTTC CTACGGGCAT CTGTCAGCGG GTGCCCTCAG CCCAGCCTTC 
ACCTTCCCCC ACCCCATCAA CCCCGTGGCC TACCAGCAGA TTCTGAGCCA GCAGAGGGGT 
CTGGGGTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CCTCACCCAC CTTCCTGGCC 
CAGCAGCCCA TGGCCCTCAC CTCCATCAAT GCCACGCCCA CCCAGCTCAG CAGCAGCAGC 
AACTGTCTGA GTGACACCAA CCAGAACAAG CAGAGCAGTG AGTCGGCCGT CAGCAGCACC 
GTCAACCCTG TCGCCATTCA CAAGCGCAGC AAGGTCAAGA CCGAGCCTGA GGGCCTGCGG 
CCGGCCTCCC CTCTGGCGCT GACGCAGGGC CAGGTGTCTG GACACGGCTC ATGTGGGTGT 
GCCCTTCCCC TCTCCCAGGA GCAGCTGGCT GACCTCAAGG AAGATCTGGA CAGGGATGAC 
TGTAAGCAGG AGGCTGAGGT GGTCATCTAT GAGACCAACT GCCACTGGGA AGACTGCACC 
AAGGAGTACG ACACCCAGGA GCAGCTGGTG CATCACATCA ACAACGAGCA CATCCACGGG 
GAGAAGAAGG AGTTTGTGTG CCGCTGGCAG GCCTGCACGC GGGAGCAGAA GCCCTTCAAG 
GCGCAGTACA TGCTGGTGGT GCACATGCGG CGACACACGG GCGAGAAGCC CCACAAGTGC 
ACGTTCGAGG GCTGCTCGAA GGCCTACTCC CGCCTGGAGA ACCTGAAGAC ACACCTGCGG 
TCCCACACCG GGGAGAAGCC ATATGTGTGT GAGCACGAGG GCTGCAACAA AGCCTTCTCC 
AACGCCTCGG ACCGCGCCAA GCACCAGAAT CGCACCCACT CCAACGAGAA ACCCTACATC 
TGCAAGATCC CAGGCTGCAC CAAGAGATAC ACAGACCCCA GCTCTCTCCG GAAGCATGTG 
AAAACGGTCC ACGGCCCAGA TGCCCACGTC ACCAAGAAGC AGCGCAATGA CGTGCACCTC 
CGCACACCGC TGCTCAAAGA GAATGGGGAC AGTGAGGCCG GCACGGAGCC TGGCGGCCCA 
GAGAGCACCG AGGCCAGCAG CACCAGCCAG GCCGTGGAGG ACTGCCTGCA CGTCAGAGCC 
ATCAAGACCG AGAGCTCCGG GCTGTGTCAG TCCAGCCCCG GGGCCCAGTC GTCCTGCAGC 
AGCGAGCCCT CTCCTCTGGG CAGTGCCCCC AACAATGACA GTGGCGTGGA GATGCCGGGG 
ACGGGGCCCG GGAGCCTGGG AGACCTGACG GCACTGGATG ACACACCCCC AGGGGCCGAC 
ACCTCAGCCC TGGCTGCCCC CTCCGCTGGT GGCCTCCAGC TGCGCAAACA CATGACCACC 
ATGCACCGGT TCGAGCAGCT CAAGAAGGAG AAGCTCAAGT CACTCAAGGA TTCCTGCTCA 
TGGGCCGGGC CGACTCCACA CACGCGGAAC ACCAAGCTGC CTCCCCTCCC GGGAAGTGGC 
TCCATCCTGG AAAACTTCAG TGGCAGTGGG GGCGGCGGGC CCGCGGGGCT GCTGCCGAAC 
CCGCGGCTGT CGGAGCTGTC CGCGAGCGAG GTGACCATGC TGAGCCAGCT GCAGGAGCGC 
CGCGACAGCT CCACCAGCAC GGTCAGCTCG GCCTACACCG TGAGCCGCCG CTCCTCCGGC 
ATCTCCCCCT ACTTCTCCAG CCGCCGCTCC AGCGAGGCCT CGCCCCTGGG CGCCGGCCGC 
CCGCACAACG CGAGCTCCGC TGACTCCTAC GACCCCATCT CCACGGACGC GTCGCGGCGC 
TCGAGCGAGG CCAGCCAGTG CAGCGGCGGC TCCGGGCTGC TCAACCTCAC GCCGGCGCAG 
CAGTACAGCC TGCGGGCCAA GTACGCGGCA GCCACTGGCG GCCCCCCGCC CACTCCGCTG 
CCGGGCCTGG AGCGCATGAG CCTGCGGACC AGGCTGGCGC TGCTGGACGC GCCCGAGCGC 
ACGCTGCCCG CCGGCTGCCC ACGCCCACTG GGGCCGCGGC GTGGCAGCGA CGGGCCGACC 
TATGGCCACG GCCACGCGGG GGCTGCGCCC GCCTTCCCCC ACGAGGCTCC AGGCGGCGGA 
GCCAGGCGGG CCAGCGACCC TGTGCGGCGG CCCGATGCCC TGTCCCTGCC GCGGGTGCAG 
CGCTTCCACA GCACCCACAA CGTGAACCCC GGCCCGCTGC CGCCCTGTGC CGACAGGCGA 
GGCCTCCGCC TGCAGAGCCA CCCGAGCACC GACGGCGGCC TGGCCCGCGG CGCCTACTCG 
CCCCGGCCGC CTAGCATCAG CGAGAACGTG GCGATGGAGG CCGTGGCGGC AGGAGTGGAC 
GGCGCGGGGC CCGAGGCCGA CCTGGGGCTG CCGGAGGACG ACCTGGTGCT TCCAGACGAC 
GTGGTGCAGT ACATCAAGGC GCACGCCAGT GGCGCTCTGG ACGAGGGCAC CGGGCAGGTG 
TATCCCACGG AAAGCACTGG CTTCTCTGAC AACCCCAGAC TACCCAGCCC GGGGCTGCAC 
GGCCAGCGCA GGATGGTGGC TGCGGACTCC AACGTGGGCC CCTCCGCCCC TATGCTGGGA 
GGATGCCAGT TAGGCTTTGG GGCGCCCTCC AGCCTGAACA AAAATAACAT GCCTGTGCAG 
TGGAATGAGG TGAGCTCCGG CACCGTAGAC GCCCTGGCCA GCCAGGTGAA GCCTCCACCC 
TTTCCTCAGG GCAACCTGGC GGTGGTGCAG CAGAAGCCTG CCTTTGGCCA GTACCCGGGC 
TACAGTCCGC AAGGCCTACA GGCTAGCCCT GGGGGCCTGG ACAGCACGCA GCCACACCTG 
CAGCCCCGCA GCGGAGCCCC CTCCCAGGGC ATCCCCAGGG TAAACTACAT GCAGCAGCTG 
CGACAGCCAG TGGCAGGCAG CCAGTGTCCT GGCATGACTA CCACTATGAG CCCCCATGCC 
TGCTATGGCC AAGTCCACCC CCAGCTGAGC CCCAGCACCA TCAGTGGGGC CCTCAACCAG 
TTCCCCCAAT CCTGCAGCAA CATGCCAGCC AAGCCAGGGC ATCTGGGGCA CCCTCAGCAG 
ACAGAAGTGG CACCTGACCC CACCACGATG GGCAATCGCC ACAGGGAACT TGGGGTCCCC 
GATTCAGCCC TGGCTGGAGT GCCACCACCT CACCCAGTCC AGAGCTACCC ACAGCAGAGC 
CATCACCTGG CAGCCTCCAT GAGCCAGGAG GGCTACCACC AGGTCCCCAG CCTTCTGCCT 
GCCCGCCAGC CTGGCTTCAT GGAGCCCCAA ACAGGCCCGA TGGGGGTGGC TACAGCAGGC 
TTTGGCCTAG TGCAGCCCCG GCCTCCCCTC GAGCCCAGCC CCACTGGCCG CCACCGTGGG 
GTACGTGCTG TGCAGCAGCA GCTGGCCTAC GCCAGGGCCA CAGGCCATGC CATGGCTGCC 
ATGCCGTCCA GTCAGGAAAC AGCAGAGGCT GTGCCCAAGG GAGCGATGGG CAACATGGGG 
TCGGTGCCTC CCCAGCCGCC TCCGCAGGAC GCAGGTGGGG CCCCGGACCA CAGCATGCTC 
TACTACTACG GCCAGATCCA CATGTACGAA CAGGATGGAG GCCTGGAGAA CCTCGGGAGC 
TGCCAGGTCA TGCGGTCCCA GCCACCACAG CCACAGGCCT GTCAGGACAG CATCCAGCCC 
CAGCCCTTGC CCTCACCAGG GGTCAACCAG GTGTCCAGCA CTGTGGACTC CCAGCTCCTG 
GAGGCCCCCC AGATTGACTT CGATGCCATC ATGGATGATG GCGATCACTC GAGTTTGTTC 
TCGGGTGCTC TGAGCCCCAG CCTCCTCCAC AGCCTCTCCC AGAACTCCTC CCGCCTCACC 
ACCCCCCGAA ACTCCTTGAC CCTGCCCTCC ATCCCCGCAG GCATCAGCAA CATGGCTGTC 
GGGGACATGA GCTCCATGCT CACCAGCCTC GCCGAGGAGA GCAAGTTCCT GAACATGATG 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509275.1
CDS	187..4572
Translation	MEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQGQVSGHGSCGCALPLSQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	XM_011510973.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510973.2

ORF Insert Sequence:

ATGGAGCACT ACCTCCGTTC TGTGCACAGC AGCCCCACGC TCTCCATGAT CTCTGCAGCC 
AGGGGCCTCA GCCCCGCTGA TGTGGCCCAG GAGCACCTTA AGGAGAGGGG ACTGTTTGGC 
CTTCCTGCTC CAGGCACCAC CCCCTCAGAC TATTACCACC AGATGACCCT CGTGGCAGGC 
CACCCCGCGC CCTACGGGGA CCTGCTGATG CAGAGCGGGG GCGCTGCCAG CGCACCCCAT 
CTCCACGACT ACCTCAACCC CGTGGACGTG TCCCGTTTCT CCAGCCCGCG GGTGACGCCC 
CGCCTGAGCC GCAAGCGGGC GCTGTCCATC TCCCCACTCT CAGACGCCAG CCTGGACCTG 
CAGCGGATGA TCCGCACCTC ACCCAACTCG CTAGTGGCCT ACATCAACAA CTCCCGAAGC 
AGCTCGGCGG CCAGCGGTTC CTACGGGCAT CTGTCAGCGG GTGCCCTCAG CCCAGCCTTC 
ACCTTCCCCC ACCCCATCAA CCCCGTGGCC TACCAGCAGA TTCTGAGCCA GCAGAGGGGT 
CTGGGGTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CCTCACCCAC CTTCCTGGCC 
CAGCAGCCCA TGGCCCTCAC CTCCATCAAT GCCACGCCCA CCCAGCTCAG CAGCAGCAGC 
AACTGTCTGA GTGACACCAA CCAGAACAAG CAGAGCAGTG AGTCGGCCGT CAGCAGCACC 
GTCAACCCTG TCGCCATTCA CAAGCGCAGC AAGGTCAAGA CCGAGCCTGA GGGCCTGCGG 
CCGGCCTCCC CTCTGGCGCT GACGCAGGGC CAGGTGTCTG GACACGGCTC ATGTGGGTGT 
GCCCTTCCCC TCTCCCAGGA GCAGCTGGCT GACCTCAAGG AAGATCTGGA CAGGGATGAC 
TGTAAGCAGG AGGCTGAGGT GGTCATCTAT GAGACCAACT GCCACTGGGA AGACTGCACC 
AAGGAGTACG ACACCCAGGA GCAGCTGGTG CATCACATCA ACAACGAGCA CATCCACGGG 
GAGAAGAAGG AGTTTGTGTG CCGCTGGCAG GCCTGCACGC GGGAGCAGAA GCCCTTCAAG 
GCGCAGTACA TGCTGGTGGT GCACATGCGG CGACACACGG GCGAGAAGCC CCACAAGTGC 
ACGTTCGAGG GCTGCTCGAA GGCCTACTCC CGCCTGGAGA ACCTGAAGAC ACACCTGCGG 
TCCCACACCG GGGAGAAGCC ATATGTGTGT GAGCACGAGG GCTGCAACAA AGCCTTCTCC 
AACGCCTCGG ACCGCGCCAA GCACCAGAAT CGCACCCACT CCAACGAGAA ACCCTACATC 
TGCAAGATCC CAGGCTGCAC CAAGAGATAC ACAGACCCCA GCTCTCTCCG GAAGCATGTG 
AAAACGGTCC ACGGCCCAGA TGCCCACGTC ACCAAGAAGC AGCGCAATGA CGTGCACCTC 
CGCACACCGC TGCTCAAAGA GAATGGGGAC AGTGAGGCCG GCACGGAGCC TGGCGGCCCA 
GAGAGCACCG AGGCCAGCAG CACCAGCCAG GCCGTGGAGG ACTGCCTGCA CGTCAGAGCC 
ATCAAGACCG AGAGCTCCGG GCTGTGTCAG TCCAGCCCCG GGGCCCAGTC GTCCTGCAGC 
AGCGAGCCCT CTCCTCTGGG CAGTGCCCCC AACAATGACA GTGGCGTGGA GATGCCGGGG 
ACGGGGCCCG GGAGCCTGGG AGACCTGACG GCACTGGATG ACACACCCCC AGGGGCCGAC 
ACCTCAGCCC TGGCTGCCCC CTCCGCTGGT GGCCTCCAGC TGCGCAAACA CATGACCACC 
ATGCACCGGT TCGAGCAGCT CAAGAAGGAG AAGCTCAAGT CACTCAAGGA TTCCTGCTCA 
TGGGCCGGGC CGACTCCACA CACGCGGAAC ACCAAGCTGC CTCCCCTCCC GGGAAGTGGC 
TCCATCCTGG AAAACTTCAG TGGCAGTGGG GGCGGCGGGC CCGCGGGGCT GCTGCCGAAC 
CCGCGGCTGT CGGAGCTGTC CGCGAGCGAG GTGACCATGC TGAGCCAGCT GCAGGAGCGC 
CGCGACAGCT CCACCAGCAC GGTCAGCTCG GCCTACACCG TGAGCCGCCG CTCCTCCGGC 
ATCTCCCCCT ACTTCTCCAG CCGCCGCTCC AGCGAGGCCT CGCCCCTGGG CGCCGGCCGC 
CCGCACAACG CGAGCTCCGC TGACTCCTAC GACCCCATCT CCACGGACGC GTCGCGGCGC 
TCGAGCGAGG CCAGCCAGTG CAGCGGCGGC TCCGGGCTGC TCAACCTCAC GCCGGCGCAG 
CAGTACAGCC TGCGGGCCAA GTACGCGGCA GCCACTGGCG GCCCCCCGCC CACTCCGCTG 
CCGGGCCTGG AGCGCATGAG CCTGCGGACC AGGCTGGCGC TGCTGGACGC GCCCGAGCGC 
ACGCTGCCCG CCGGCTGCCC ACGCCCACTG GGGCCGCGGC GTGGCAGCGA CGGGCCGACC 
TATGGCCACG GCCACGCGGG GGCTGCGCCC GCCTTCCCCC ACGAGGCTCC AGGCGGCGGA 
GCCAGGCGGG CCAGCGACCC TGTGCGGCGG CCCGATGCCC TGTCCCTGCC GCGGGTGCAG 
CGCTTCCACA GCACCCACAA CGTGAACCCC GGCCCGCTGC CGCCCTGTGC CGACAGGCGA 
GGCCTCCGCC TGCAGAGCCA CCCGAGCACC GACGGCGGCC TGGCCCGCGG CGCCTACTCG 
CCCCGGCCGC CTAGCATCAG CGAGAACGTG GCGATGGAGG CCGTGGCGGC AGGAGTGGAC 
GGCGCGGGGC CCGAGGCCGA CCTGGGGCTG CCGGAGGACG ACCTGGTGCT TCCAGACGAC 
GTGGTGCAGT ACATCAAGGC GCACGCCAGT GGCGCTCTGG ACGAGGGCAC CGGGCAGGTG 
TATCCCACGG AAAGCACTGG CTTCTCTGAC AACCCCAGAC TACCCAGCCC GGGGCTGCAC 
GGCCAGCGCA GGATGGTGGC TGCGGACTCC AACGTGGGCC CCTCCGCCCC TATGCTGGGA 
GGATGCCAGT TAGGCTTTGG GGCGCCCTCC AGCCTGAACA AAAATAACAT GCCTGTGCAG 
TGGAATGAGG TGAGCTCCGG CACCGTAGAC GCCCTGGCCA GCCAGGTGAA GCCTCCACCC 
TTTCCTCAGG GCAACCTGGC GGTGGTGCAG CAGAAGCCTG CCTTTGGCCA GTACCCGGGC 
TACAGTCCGC AAGGCCTACA GGCTAGCCCT GGGGGCCTGG ACAGCACGCA GCCACACCTG 
CAGCCCCGCA GCGGAGCCCC CTCCCAGGGC ATCCCCAGGG TAAACTACAT GCAGCAGCTG 
CGACAGCCAG TGGCAGGCAG CCAGTGTCCT GGCATGACTA CCACTATGAG CCCCCATGCC 
TGCTATGGCC AAGTCCACCC CCAGCTGAGC CCCAGCACCA TCAGTGGGGC CCTCAACCAG 
TTCCCCCAAT CCTGCAGCAA CATGCCAGCC AAGCCAGGGC ATCTGGGGCA CCCTCAGCAG 
ACAGAAGTGG CACCTGACCC CACCACGATG GGCAATCGCC ACAGGGAACT TGGGGTCCCC 
GATTCAGCCC TGGCTGGAGT GCCACCACCT CACCCAGTCC AGAGCTACCC ACAGCAGAGC 
CATCACCTGG CAGCCTCCAT GAGCCAGGAG GGCTACCACC AGGTCCCCAG CCTTCTGCCT 
GCCCGCCAGC CTGGCTTCAT GGAGCCCCAA ACAGGCCCGA TGGGGGTGGC TACAGCAGGC 
TTTGGCCTAG TGCAGCCCCG GCCTCCCCTC GAGCCCAGCC CCACTGGCCG CCACCGTGGG 
GTACGTGCTG TGCAGCAGCA GCTGGCCTAC GCCAGGGCCA CAGGCCATGC CATGGCTGCC 
ATGCCGTCCA GTCAGGAAAC AGCAGAGGCT GTGCCCAAGG GAGCGATGGG CAACATGGGG 
TCGGTGCCTC CCCAGCCGCC TCCGCAGGAC GCAGGTGGGG CCCCGGACCA CAGCATGCTC 
TACTACTACG GCCAGATCCA CATGTACGAA CAGGATGGAG GCCTGGAGAA CCTCGGGAGC 
TGCCAGGTCA TGCGGTCCCA GCCACCACAG CCACAGGCCT GTCAGGACAG CATCCAGCCC 
CAGCCCTTGC CCTCACCAGG GGTCAACCAG GTGTCCAGCA CTGTGGACTC CCAGCTCCTG 
GAGGCCCCCC AGATTGACTT CGATGCCATC ATGGATGATG GCGATCACTC GAGTTTGTTC 
TCGGGTGCTC TGAGCCCCAG CCTCCTCCAC AGCCTCTCCC AGAACTCCTC CCGCCTCACC 
ACCCCCCGAA ACTCCTTGAC CCTGCCCTCC ATCCCCGCAG GCATCAGCAA CATGGCTGTC 
GGGGACATGA GCTCCATGCT CACCAGCCTC GCCGAGGAGA GCAAGTTCCT GAACATGATG 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( XM_011510974.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509276.1 Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X10, mRNA.

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CloneID	OHu57986
Clone ID Related Accession (Same CDS sequence)	XM_011510974.2 , NM_001374354.1
Accession Version	XM_011510974.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	zinc finger protein GLI2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510974.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGAGCACT ACCTCCGTTC TGTGCACAGC AGCCCCACGC TCTCCATGAT CTCTGCAGCC 
AGGGGCCTCA GCCCCGCTGA TGTGGCCCAG GAGCACCTTA AGGAGAGGGG ACTGTTTGGC 
CTTCCTGCTC CAGGCACCAC CCCCTCAGAC TATTACCACC AGATGACCCT CGTGGCAGGC 
CACCCCGCGC CCTACGGGGA CCTGCTGATG CAGAGCGGGG GCGCTGCCAG CGCACCCCAT 
CTCCACGACT ACCTCAACCC CGTGGACGTG TCCCGTTTCT CCAGCCCGCG GGTGACGCCC 
CGCCTGAGCC GCAAGCGGGC GCTGTCCATC TCCCCACTCT CAGACGCCAG CCTGGACCTG 
CAGCGGATGA TCCGCACCTC ACCCAACTCG CTAGTGGCCT ACATCAACAA CTCCCGAAGC 
AGCTCGGCGG CCAGCGGTTC CTACGGGCAT CTGTCAGCGG GTGCCCTCAG CCCAGCCTTC 
ACCTTCCCCC ACCCCATCAA CCCCGTGGCC TACCAGCAGA TTCTGAGCCA GCAGAGGGGT 
CTGGGGTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CCTCACCCAC CTTCCTGGCC 
CAGCAGCCCA TGGCCCTCAC CTCCATCAAT GCCACGCCCA CCCAGCTCAG CAGCAGCAGC 
AACTGTCTGA GTGACACCAA CCAGAACAAG CAGAGCAGTG AGTCGGCCGT CAGCAGCACC 
GTCAACCCTG TCGCCATTCA CAAGCGCAGC AAGGTCAAGA CCGAGCCTGA GGGCCTGCGG 
CCGGCCTCCC CTCTGGCGCT GACGCAGGAG CAGCTGGCTG ACCTCAAGGA AGATCTGGAC 
AGGGATGACT GTAAGCAGGA GGCTGAGGTG GTCATCTATG AGACCAACTG CCACTGGGAA 
GACTGCACCA AGGAGTACGA CACCCAGGAG CAGCTGGTGC ATCACATCAA CAACGAGCAC 
ATCCACGGGG AGAAGAAGGA GTTTGTGTGC CGCTGGCAGG CCTGCACGCG GGAGCAGAAG 
CCCTTCAAGG CGCAGTACAT GCTGGTGGTG CACATGCGGC GACACACGGG CGAGAAGCCC 
CACAAGTGCA CGTTCGAGGG CTGCTCGAAG GCCTACTCCC GCCTGGAGAA CCTGAAGACA 
CACCTGCGGT CCCACACCGG GGAGAAGCCA TATGTGTGTG AGCACGAGGG CTGCAACAAA 
GCCTTCTCCA ACGCCTCGGA CCGCGCCAAG CACCAGAATC GCACCCACTC CAACGAGAAA 
CCCTACATCT GCAAGATCCC AGGCTGCACC AAGAGATACA CAGACCCCAG CTCTCTCCGG 
AAGCATGTGA AAACGGTCCA CGGCCCAGAT GCCCACGTCA CCAAGAAGCA GCGCAATGAC 
GTGCACCTCC GCACACCGCT GCTCAAAGAG AATGGGGACA GTGAGGCCGG CACGGAGCCT 
GGCGGCCCAG AGAGCACCGA GGCCAGCAGC ACCAGCCAGG CCGTGGAGGA CTGCCTGCAC 
GTCAGAGCCA TCAAGACCGA GAGCTCCGGG CTGTGTCAGT CCAGCCCCGG GGCCCAGTCG 
TCCTGCAGCA GCGAGCCCTC TCCTCTGGGC AGTGCCCCCA ACAATGACAG TGGCGTGGAG 
ATGCCGGGGA CGGGGCCCGG GAGCCTGGGA GACCTGACGG CACTGGATGA CACACCCCCA 
GGGGCCGACA CCTCAGCCCT GGCTGCCCCC TCCGCTGGTG GCCTCCAGCT GCGCAAACAC 
ATGACCACCA TGCACCGGTT CGAGCAGCTC AAGAAGGAGA AGCTCAAGTC ACTCAAGGAT 
TCCTGCTCAT GGGCCGGGCC GACTCCACAC ACGCGGAACA CCAAGCTGCC TCCCCTCCCG 
GGAAGTGGCT CCATCCTGGA AAACTTCAGT GGCAGTGGGG GCGGCGGGCC CGCGGGGCTG 
CTGCCGAACC CGCGGCTGTC GGAGCTGTCC GCGAGCGAGG TGACCATGCT GAGCCAGCTG 
CAGGAGCGCC GCGACAGCTC CACCAGCACG GTCAGCTCGG CCTACACCGT GAGCCGCCGC 
TCCTCCGGCA TCTCCCCCTA CTTCTCCAGC CGCCGCTCCA GCGAGGCCTC GCCCCTGGGC 
GCCGGCCGCC CGCACAACGC GAGCTCCGCT GACTCCTACG ACCCCATCTC CACGGACGCG 
TCGCGGCGCT CGAGCGAGGC CAGCCAGTGC AGCGGCGGCT CCGGGCTGCT CAACCTCACG 
CCGGCGCAGC AGTACAGCCT GCGGGCCAAG TACGCGGCAG CCACTGGCGG CCCCCCGCCC 
ACTCCGCTGC CGGGCCTGGA GCGCATGAGC CTGCGGACCA GGCTGGCGCT GCTGGACGCG 
CCCGAGCGCA CGCTGCCCGC CGGCTGCCCA CGCCCACTGG GGCCGCGGCG TGGCAGCGAC 
GGGCCGACCT ATGGCCACGG CCACGCGGGG GCTGCGCCCG CCTTCCCCCA CGAGGCTCCA 
GGCGGCGGAG CCAGGCGGGC CAGCGACCCT GTGCGGCGGC CCGATGCCCT GTCCCTGCCG 
CGGGTGCAGC GCTTCCACAG CACCCACAAC GTGAACCCCG GCCCGCTGCC GCCCTGTGCC 
GACAGGCGAG GCCTCCGCCT GCAGAGCCAC CCGAGCACCG ACGGCGGCCT GGCCCGCGGC 
GCCTACTCGC CCCGGCCGCC TAGCATCAGC GAGAACGTGG CGATGGAGGC CGTGGCGGCA 
GGAGTGGACG GCGCGGGGCC CGAGGCCGAC CTGGGGCTGC CGGAGGACGA CCTGGTGCTT 
CCAGACGACG TGGTGCAGTA CATCAAGGCG CACGCCAGTG GCGCTCTGGA CGAGGGCACC 
GGGCAGGTGT ATCCCACGGA AAGCACTGGC TTCTCTGACA ACCCCAGACT ACCCAGCCCG 
GGGCTGCACG GCCAGCGCAG GATGGTGGCT GCGGACTCCA ACGTGGGCCC CTCCGCCCCT 
ATGCTGGGAG GATGCCAGTT AGGCTTTGGG GCGCCCTCCA GCCTGAACAA AAATAACATG 
CCTGTGCAGT GGAATGAGGT GAGCTCCGGC ACCGTAGACG CCCTGGCCAG CCAGGTGAAG 
CCTCCACCCT TTCCTCAGGG CAACCTGGCG GTGGTGCAGC AGAAGCCTGC CTTTGGCCAG 
TACCCGGGCT ACAGTCCGCA AGGCCTACAG GCTAGCCCTG GGGGCCTGGA CAGCACGCAG 
CCACACCTGC AGCCCCGCAG CGGAGCCCCC TCCCAGGGCA TCCCCAGGGT AAACTACATG 
CAGCAGCTGC GACAGCCAGT GGCAGGCAGC CAGTGTCCTG GCATGACTAC CACTATGAGC 
CCCCATGCCT GCTATGGCCA AGTCCACCCC CAGCTGAGCC CCAGCACCAT CAGTGGGGCC 
CTCAACCAGT TCCCCCAATC CTGCAGCAAC ATGCCAGCCA AGCCAGGGCA TCTGGGGCAC 
CCTCAGCAGA CAGAAGTGGC ACCTGACCCC ACCACGATGG GCAATCGCCA CAGGGAACTT 
GGGGTCCCCG ATTCAGCCCT GGCTGGAGTG CCACCACCTC ACCCAGTCCA GAGCTACCCA 
CAGCAGAGCC ATCACCTGGC AGCCTCCATG AGCCAGGAGG GCTACCACCA GGTCCCCAGC 
CTTCTGCCTG CCCGCCAGCC TGGCTTCATG GAGCCCCAAA CAGGCCCGAT GGGGGTGGCT 
ACAGCAGGCT TTGGCCTAGT GCAGCCCCGG CCTCCCCTCG AGCCCAGCCC CACTGGCCGC 
CACCGTGGGG TACGTGCTGT GCAGCAGCAG CTGGCCTACG CCAGGGCCAC AGGCCATGCC 
ATGGCTGCCA TGCCGTCCAG TCAGGAAACA GCAGAGGCTG TGCCCAAGGG AGCGATGGGC 
AACATGGGGT CGGTGCCTCC CCAGCCGCCT CCGCAGGACG CAGGTGGGGC CCCGGACCAC 
AGCATGCTCT ACTACTACGG CCAGATCCAC ATGTACGAAC AGGATGGAGG CCTGGAGAAC 
CTCGGGAGCT GCCAGGTCAT GCGGTCCCAG CCACCACAGC CACAGGCCTG TCAGGACAGC 
ATCCAGCCCC AGCCCTTGCC CTCACCAGGG GTCAACCAGG TGTCCAGCAC TGTGGACTCC 
CAGCTCCTGG AGGCCCCCCA GATTGACTTC GATGCCATCA TGGATGATGG CGATCACTCG 
AGTTTGTTCT CGGGTGCTCT GAGCCCCAGC CTCCTCCACA GCCTCTCCCA GAACTCCTCC 
CGCCTCACCA CCCCCCGAAA CTCCTTGACC CTGCCCTCCA TCCCCGCAGG CATCAGCAAC 
ATGGCTGTCG GGGACATGAG CTCCATGCTC ACCAGCCTCG CCGAGGAGAG CAAGTTCCTG 
AACATGATGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509276.1
CDS	246..4580
Translation	MEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	XM_011510974.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510974.2

ORF Insert Sequence:

ATGGAGCACT ACCTCCGTTC TGTGCACAGC AGCCCCACGC TCTCCATGAT CTCTGCAGCC 
AGGGGCCTCA GCCCCGCTGA TGTGGCCCAG GAGCACCTTA AGGAGAGGGG ACTGTTTGGC 
CTTCCTGCTC CAGGCACCAC CCCCTCAGAC TATTACCACC AGATGACCCT CGTGGCAGGC 
CACCCCGCGC CCTACGGGGA CCTGCTGATG CAGAGCGGGG GCGCTGCCAG CGCACCCCAT 
CTCCACGACT ACCTCAACCC CGTGGACGTG TCCCGTTTCT CCAGCCCGCG GGTGACGCCC 
CGCCTGAGCC GCAAGCGGGC GCTGTCCATC TCCCCACTCT CAGACGCCAG CCTGGACCTG 
CAGCGGATGA TCCGCACCTC ACCCAACTCG CTAGTGGCCT ACATCAACAA CTCCCGAAGC 
AGCTCGGCGG CCAGCGGTTC CTACGGGCAT CTGTCAGCGG GTGCCCTCAG CCCAGCCTTC 
ACCTTCCCCC ACCCCATCAA CCCCGTGGCC TACCAGCAGA TTCTGAGCCA GCAGAGGGGT 
CTGGGGTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CCTCACCCAC CTTCCTGGCC 
CAGCAGCCCA TGGCCCTCAC CTCCATCAAT GCCACGCCCA CCCAGCTCAG CAGCAGCAGC 
AACTGTCTGA GTGACACCAA CCAGAACAAG CAGAGCAGTG AGTCGGCCGT CAGCAGCACC 
GTCAACCCTG TCGCCATTCA CAAGCGCAGC AAGGTCAAGA CCGAGCCTGA GGGCCTGCGG 
CCGGCCTCCC CTCTGGCGCT GACGCAGGAG CAGCTGGCTG ACCTCAAGGA AGATCTGGAC 
AGGGATGACT GTAAGCAGGA GGCTGAGGTG GTCATCTATG AGACCAACTG CCACTGGGAA 
GACTGCACCA AGGAGTACGA CACCCAGGAG CAGCTGGTGC ATCACATCAA CAACGAGCAC 
ATCCACGGGG AGAAGAAGGA GTTTGTGTGC CGCTGGCAGG CCTGCACGCG GGAGCAGAAG 
CCCTTCAAGG CGCAGTACAT GCTGGTGGTG CACATGCGGC GACACACGGG CGAGAAGCCC 
CACAAGTGCA CGTTCGAGGG CTGCTCGAAG GCCTACTCCC GCCTGGAGAA CCTGAAGACA 
CACCTGCGGT CCCACACCGG GGAGAAGCCA TATGTGTGTG AGCACGAGGG CTGCAACAAA 
GCCTTCTCCA ACGCCTCGGA CCGCGCCAAG CACCAGAATC GCACCCACTC CAACGAGAAA 
CCCTACATCT GCAAGATCCC AGGCTGCACC AAGAGATACA CAGACCCCAG CTCTCTCCGG 
AAGCATGTGA AAACGGTCCA CGGCCCAGAT GCCCACGTCA CCAAGAAGCA GCGCAATGAC 
GTGCACCTCC GCACACCGCT GCTCAAAGAG AATGGGGACA GTGAGGCCGG CACGGAGCCT 
GGCGGCCCAG AGAGCACCGA GGCCAGCAGC ACCAGCCAGG CCGTGGAGGA CTGCCTGCAC 
GTCAGAGCCA TCAAGACCGA GAGCTCCGGG CTGTGTCAGT CCAGCCCCGG GGCCCAGTCG 
TCCTGCAGCA GCGAGCCCTC TCCTCTGGGC AGTGCCCCCA ACAATGACAG TGGCGTGGAG 
ATGCCGGGGA CGGGGCCCGG GAGCCTGGGA GACCTGACGG CACTGGATGA CACACCCCCA 
GGGGCCGACA CCTCAGCCCT GGCTGCCCCC TCCGCTGGTG GCCTCCAGCT GCGCAAACAC 
ATGACCACCA TGCACCGGTT CGAGCAGCTC AAGAAGGAGA AGCTCAAGTC ACTCAAGGAT 
TCCTGCTCAT GGGCCGGGCC GACTCCACAC ACGCGGAACA CCAAGCTGCC TCCCCTCCCG 
GGAAGTGGCT CCATCCTGGA AAACTTCAGT GGCAGTGGGG GCGGCGGGCC CGCGGGGCTG 
CTGCCGAACC CGCGGCTGTC GGAGCTGTCC GCGAGCGAGG TGACCATGCT GAGCCAGCTG 
CAGGAGCGCC GCGACAGCTC CACCAGCACG GTCAGCTCGG CCTACACCGT GAGCCGCCGC 
TCCTCCGGCA TCTCCCCCTA CTTCTCCAGC CGCCGCTCCA GCGAGGCCTC GCCCCTGGGC 
GCCGGCCGCC CGCACAACGC GAGCTCCGCT GACTCCTACG ACCCCATCTC CACGGACGCG 
TCGCGGCGCT CGAGCGAGGC CAGCCAGTGC AGCGGCGGCT CCGGGCTGCT CAACCTCACG 
CCGGCGCAGC AGTACAGCCT GCGGGCCAAG TACGCGGCAG CCACTGGCGG CCCCCCGCCC 
ACTCCGCTGC CGGGCCTGGA GCGCATGAGC CTGCGGACCA GGCTGGCGCT GCTGGACGCG 
CCCGAGCGCA CGCTGCCCGC CGGCTGCCCA CGCCCACTGG GGCCGCGGCG TGGCAGCGAC 
GGGCCGACCT ATGGCCACGG CCACGCGGGG GCTGCGCCCG CCTTCCCCCA CGAGGCTCCA 
GGCGGCGGAG CCAGGCGGGC CAGCGACCCT GTGCGGCGGC CCGATGCCCT GTCCCTGCCG 
CGGGTGCAGC GCTTCCACAG CACCCACAAC GTGAACCCCG GCCCGCTGCC GCCCTGTGCC 
GACAGGCGAG GCCTCCGCCT GCAGAGCCAC CCGAGCACCG ACGGCGGCCT GGCCCGCGGC 
GCCTACTCGC CCCGGCCGCC TAGCATCAGC GAGAACGTGG CGATGGAGGC CGTGGCGGCA 
GGAGTGGACG GCGCGGGGCC CGAGGCCGAC CTGGGGCTGC CGGAGGACGA CCTGGTGCTT 
CCAGACGACG TGGTGCAGTA CATCAAGGCG CACGCCAGTG GCGCTCTGGA CGAGGGCACC 
GGGCAGGTGT ATCCCACGGA AAGCACTGGC TTCTCTGACA ACCCCAGACT ACCCAGCCCG 
GGGCTGCACG GCCAGCGCAG GATGGTGGCT GCGGACTCCA ACGTGGGCCC CTCCGCCCCT 
ATGCTGGGAG GATGCCAGTT AGGCTTTGGG GCGCCCTCCA GCCTGAACAA AAATAACATG 
CCTGTGCAGT GGAATGAGGT GAGCTCCGGC ACCGTAGACG CCCTGGCCAG CCAGGTGAAG 
CCTCCACCCT TTCCTCAGGG CAACCTGGCG GTGGTGCAGC AGAAGCCTGC CTTTGGCCAG 
TACCCGGGCT ACAGTCCGCA AGGCCTACAG GCTAGCCCTG GGGGCCTGGA CAGCACGCAG 
CCACACCTGC AGCCCCGCAG CGGAGCCCCC TCCCAGGGCA TCCCCAGGGT AAACTACATG 
CAGCAGCTGC GACAGCCAGT GGCAGGCAGC CAGTGTCCTG GCATGACTAC CACTATGAGC 
CCCCATGCCT GCTATGGCCA AGTCCACCCC CAGCTGAGCC CCAGCACCAT CAGTGGGGCC 
CTCAACCAGT TCCCCCAATC CTGCAGCAAC ATGCCAGCCA AGCCAGGGCA TCTGGGGCAC 
CCTCAGCAGA CAGAAGTGGC ACCTGACCCC ACCACGATGG GCAATCGCCA CAGGGAACTT 
GGGGTCCCCG ATTCAGCCCT GGCTGGAGTG CCACCACCTC ACCCAGTCCA GAGCTACCCA 
CAGCAGAGCC ATCACCTGGC AGCCTCCATG AGCCAGGAGG GCTACCACCA GGTCCCCAGC 
CTTCTGCCTG CCCGCCAGCC TGGCTTCATG GAGCCCCAAA CAGGCCCGAT GGGGGTGGCT 
ACAGCAGGCT TTGGCCTAGT GCAGCCCCGG CCTCCCCTCG AGCCCAGCCC CACTGGCCGC 
CACCGTGGGG TACGTGCTGT GCAGCAGCAG CTGGCCTACG CCAGGGCCAC AGGCCATGCC 
ATGGCTGCCA TGCCGTCCAG TCAGGAAACA GCAGAGGCTG TGCCCAAGGG AGCGATGGGC 
AACATGGGGT CGGTGCCTCC CCAGCCGCCT CCGCAGGACG CAGGTGGGGC CCCGGACCAC 
AGCATGCTCT ACTACTACGG CCAGATCCAC ATGTACGAAC AGGATGGAGG CCTGGAGAAC 
CTCGGGAGCT GCCAGGTCAT GCGGTCCCAG CCACCACAGC CACAGGCCTG TCAGGACAGC 
ATCCAGCCCC AGCCCTTGCC CTCACCAGGG GTCAACCAGG TGTCCAGCAC TGTGGACTCC 
CAGCTCCTGG AGGCCCCCCA GATTGACTTC GATGCCATCA TGGATGATGG CGATCACTCG 
AGTTTGTTCT CGGGTGCTCT GAGCCCCAGC CTCCTCCACA GCCTCTCCCA GAACTCCTCC 
CGCCTCACCA CCCCCCGAAA CTCCTTGACC CTGCCCTCCA TCCCCGCAGG CATCAGCAAC 
ATGGCTGTCG GGGACATGAG CTCCATGCTC ACCAGCCTCG CCGAGGAGAG CAAGTTCCTG 
AACATGATGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( XM_011510969.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509271.2 Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA.

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CloneID	OHu89279
Clone ID Related Accession (Same CDS sequence)	XM_011510969.2
Accession Version	XM_011510969.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	zinc finger protein GLI2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCCA AGTTCCAGAC CCTGGAGGGC AGCCGCTGCC CCTGGAAGGC ATGCCGACGT 
CAGCTTCTCC CGGGAGGCGG AATTCATGCG GGCCCTAGTC TGAGAAAGGC ACCCGCAGGG 
TGGACAGCAT GCTGCCCGCC AGCAGTGTGG CTGGATCCCC GAGTCCAGCC AGCCTTTCCG 
TTAGCCAGCC AGCCAGCGAG CGCTCCTAAA CCCTGGCCGC CGCCGGTTAT AAATGATTCA 
TGCAGCCCCG CCTCCCTCGG CGGCCACTTG ATGTTCCCTG CGCTCCAGCC CGGCGGTCCA 
CCGCCTCTCC TGCCGCTGGG CAGTGCCGCC GCTGGGCAGT GCCCTCCCCG CACCGCCGCC 
CCTCTGGGTG CTGCGTCCCT CCGCCCTCCC CGCACGCTGG TGCCGCAGCA TCTCTTGCCA 
CCATTCCATG CGCCCCTACC GATTGACATG CGACACCAGG AAGGAAGGTA CCATTACGAG 
CCTCATTCTG TCCACGGTGT GCACGGGCCC CCTGCCCTCA GCGGCAGCCC TGTCATCTCT 
GACATCTCCT TGATCCGGCT TTCCCCGCAC CCGGCTGGCC CTGGGGAGTC CCCCTTCAAC 
GCCCCCCACC CGTACGTGAA CCCCCACATG GAGCACTACC TCCGTTCTGT GCACAGCAGC 
CCCACGCTCT CCATGATCTC TGCAGCCAGG GGCCTCAGCC CCGCTGATGT GGCCCAGGAG 
CACCTTAAGG AGAGGGGACT GTTTGGCCTT CCTGCTCCAG GCACCACCCC CTCAGACTAT 
TACCACCAGA TGACCCTCGT GGCAGGCCAC CCCGCGCCCT ACGGGGACCT GCTGATGCAG 
AGCGGGGGCG CTGCCAGCGC ACCCCATCTC CACGACTACC TCAACCCCGT GGACGTGTCC 
CGTTTCTCCA GCCCGCGGGT GACGCCCCGC CTGAGCCGCA AGCGGGCGCT GTCCATCTCC 
CCACTCTCAG ACGCCAGCCT GGACCTGCAG CGGATGATCC GCACCTCACC CAACTCGCTA 
GTGGCCTACA TCAACAACTC CCGAAGCAGC TCGGCGGCCA GCGGTTCCTA CGGGCATCTG 
TCAGCGGGTG CCCTCAGCCC AGCCTTCACC TTCCCCCACC CCATCAACCC CGTGGCCTAC 
CAGCAGATTC TGAGCCAGCA GAGGGGTCTG GGGTCAGCCT TTGGACACAC ACCACCCCTG 
ATCCAGCCCT CACCCACCTT CCTGGCCCAG CAGCCCATGG CCCTCACCTC CATCAATGCC 
ACGCCCACCC AGCTCAGCAG CAGCAGCAAC TGTCTGAGTG ACACCAACCA GAACAAGCAG 
AGCAGTGAGT CGGCCGTCAG CAGCACCGTC AACCCTGTCG CCATTCACAA GCGCAGCAAG 
GTCAAGACCG AGCCTGAGGG CCTGCGGCCG GCCTCCCCTC TGGCGCTGAC GCAGGGCCAG 
GTGTCTGGAC ACGGCTCATG TGGGTGTGCC CTTCCCCTCT CCCAGGAGCA GCTGGCTGAC 
CTCAAGGAAG ATCTGGACAG GGATGACTGT AAGCAGGAGG CTGAGGTGGT CATCTATGAG 
ACCAACTGCC ACTGGGAAGA CTGCACCAAG GAGTACGACA CCCAGGAGCA GCTGGTGCAT 
CACATCAACA ACGAGCACAT CCACGGGGAG AAGAAGGAGT TTGTGTGCCG CTGGCAGGCC 
TGCACGCGGG AGCAGAAGCC CTTCAAGGCG CAGTACATGC TGGTGGTGCA CATGCGGCGA 
CACACGGGCG AGAAGCCCCA CAAGTGCACG TTCGAGGGCT GCTCGAAGGC CTACTCCCGC 
CTGGAGAACC TGAAGACACA CCTGCGGTCC CACACCGGGG AGAAGCCATA TGTGTGTGAG 
CACGAGGGCT GCAACAAAGC CTTCTCCAAC GCCTCGGACC GCGCCAAGCA CCAGAATCGC 
ACCCACTCCA ACGAGAAACC CTACATCTGC AAGATCCCAG GCTGCACCAA GAGATACACA 
GACCCCAGCT CTCTCCGGAA GCATGTGAAA ACGGTCCACG GCCCAGATGC CCACGTCACC 
AAGAAGCAGC GCAATGACGT GCACCTCCGC ACACCGCTGC TCAAAGAGAA TGGGGACAGT 
GAGGCCGGCA CGGAGCCTGG CGGCCCAGAG AGCACCGAGG CCAGCAGCAC CAGCCAGGCC 
GTGGAGGACT GCCTGCACGT CAGAGCCATC AAGACCGAGA GCTCCGGGCT GTGTCAGTCC 
AGCCCCGGGG CCCAGTCGTC CTGCAGCAGC GAGCCCTCTC CTCTGGGCAG TGCCCCCAAC 
AATGACAGTG GCGTGGAGAT GCCGGGGACG GGGCCCGGGA GCCTGGGAGA CCTGACGGCA 
CTGGATGACA CACCCCCAGG GGCCGACACC TCAGCCCTGG CTGCCCCCTC CGCTGGTGGC 
CTCCAGCTGC GCAAACACAT GACCACCATG CACCGGTTCG AGCAGCTCAA GAAGGAGAAG 
CTCAAGTCAC TCAAGGATTC CTGCTCATGG GCCGGGCCGA CTCCACACAC GCGGAACACC 
AAGCTGCCTC CCCTCCCGGG AAGTGGCTCC ATCCTGGAAA ACTTCAGTGG CAGTGGGGGC 
GGCGGGCCCG CGGGGCTGCT GCCGAACCCG CGGCTGTCGG AGCTGTCCGC GAGCGAGGTG 
ACCATGCTGA GCCAGCTGCA GGAGCGCCGC GACAGCTCCA CCAGCACGGT CAGCTCGGCC 
TACACCGTGA GCCGCCGCTC CTCCGGCATC TCCCCCTACT TCTCCAGCCG CCGCTCCAGC 
GAGGCCTCGC CCCTGGGCGC CGGCCGCCCG CACAACGCGA GCTCCGCTGA CTCCTACGAC 
CCCATCTCCA CGGACGCGTC GCGGCGCTCG AGCGAGGCCA GCCAGTGCAG CGGCGGCTCC 
GGGCTGCTCA ACCTCACGCC GGCGCAGCAG TACAGCCTGC GGGCCAAGTA CGCGGCAGCC 
ACTGGCGGCC CCCCGCCCAC TCCGCTGCCG GGCCTGGAGC GCATGAGCCT GCGGACCAGG 
CTGGCGCTGC TGGACGCGCC CGAGCGCACG CTGCCCGCCG GCTGCCCACG CCCACTGGGG 
CCGCGGCGTG GCAGCGACGG GCCGACCTAT GGCCACGGCC ACGCGGGGGC TGCGCCCGCC 
TTCCCCCACG AGGCTCCAGG CGGCGGAGCC AGGCGGGCCA GCGACCCTGT GCGGCGGCCC 
GATGCCCTGT CCCTGCCGCG GGTGCAGCGC TTCCACAGCA CCCACAACGT GAACCCCGGC 
CCGCTGCCGC CCTGTGCCGA CAGGCGAGGC CTCCGCCTGC AGAGCCACCC GAGCACCGAC 
GGCGGCCTGG CCCGCGGCGC CTACTCGCCC CGGCCGCCTA GCATCAGCGA GAACGTGGCG 
ATGGAGGCCG TGGCGGCAGG AGTGGACGGC GCGGGGCCCG AGGCCGACCT GGGGCTGCCG 
GAGGACGACC TGGTGCTTCC AGACGACGTG GTGCAGTACA TCAAGGCGCA CGCCAGTGGC 
GCTCTGGACG AGGGCACCGG GCAGGTGTAT CCCACGGAAA GCACTGGCTT CTCTGACAAC 
CCCAGACTAC CCAGCCCGGG GCTGCACGGC CAGCGCAGGA TGGTGGCTGC GGACTCCAAC 
GTGGGCCCCT CCGCCCCTAT GCTGGGAGGA TGCCAGTTAG GCTTTGGGGC GCCCTCCAGC 
CTGAACAAAA ATAACATGCC TGTGCAGTGG AATGAGGTGA GCTCCGGCAC CGTAGACGCC 
CTGGCCAGCC AGGTGAAGCC TCCACCCTTT CCTCAGGGCA ACCTGGCGGT GGTGCAGCAG 
AAGCCTGCCT TTGGCCAGTA CCCGGGCTAC AGTCCGCAAG GCCTACAGGC TAGCCCTGGG 
GGCCTGGACA GCACGCAGCC ACACCTGCAG CCCCGCAGCG GAGCCCCCTC CCAGGGCATC 
CCCAGGGTAA ACTACATGCA GCAGCTGCGA CAGCCAGTGG CAGGCAGCCA GTGTCCTGGC 
ATGACTACCA CTATGAGCCC CCATGCCTGC TATGGCCAAG TCCACCCCCA GCTGAGCCCC 
AGCACCATCA GTGGGGCCCT CAACCAGTTC CCCCAATCCT GCAGCAACAT GCCAGCCAAG 
CCAGGGCATC TGGGGCACCC TCAGCAGACA GAAGTGGCAC CTGACCCCAC CACGATGGGC 
AATCGCCACA GGGAACTTGG GGTCCCCGAT TCAGCCCTGG CTGGAGTGCC ACCACCTCAC 
CCAGTCCAGA GCTACCCACA GCAGAGCCAT CACCTGGCAG CCTCCATGAG CCAGGAGGGC 
TACCACCAGG TCCCCAGCCT TCTGCCTGCC CGCCAGCCTG GCTTCATGGA GCCCCAAACA 
GGCCCGATGG GGGTGGCTAC AGCAGGCTTT GGCCTAGTGC AGCCCCGGCC TCCCCTCGAG 
CCCAGCCCCA CTGGCCGCCA CCGTGGGGTA CGTGCTGTGC AGCAGCAGCT GGCCTACGCC 
AGGGCCACAG GCCATGCCAT GGCTGCCATG CCGTCCAGTC AGGAAACAGC AGAGGCTGTG 
CCCAAGGGAG CGATGGGCAA CATGGGGTCG GTGCCTCCCC AGCCGCCTCC GCAGGACGCA 
GGTGGGGCCC CGGACCACAG CATGCTCTAC TACTACGGCC AGATCCACAT GTACGAACAG 
GATGGAGGCC TGGAGAACCT CGGGAGCTGC CAGGTCATGC GGTCCCAGCC ACCACAGCCA 
CAGGCCTGTC AGGACAGCAT CCAGCCCCAG CCCTTGCCCT CACCAGGGGT CAACCAGGTG 
TCCAGCACTG TGGACTCCCA GCTCCTGGAG GCCCCCCAGA TTGACTTCGA TGCCATCATG 
GATGATGGCG ATCACTCGAG TTTGTTCTCG GGTGCTCTGA GCCCCAGCCT CCTCCACAGC 
CTCTCCCAGA ACTCCTCCCG CCTCACCACC CCCCGAAACT CCTTGACCCT GCCCTCCATC 
CCCGCAGGCA TCAGCAACAT GGCTGTCGGG GACATGAGCT CCATGCTCAC CAGCCTCGCC 
GAGGAGAGCA AGTTCCTGAA CATGATGACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509271.2
CDS	411..5423
Translation	MTPKFQTLEGSRCPWKACRRQLLPGGGIHAGPSLRKAPAGWTACCPPAVWLDPRVQPAFPLASQPASAPKPWPPPVINDSCSPASLGGHLMFPALQPGGPPPLLPLGSAAAGQCPPRTAAPLGAASLRPPRTLVPQHLLPPFHAPLPIDMRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQGQVSGHGSCGCALPLSQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	XM_011510969.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510969.2

ORF Insert Sequence:

ATGACCCCCA AGTTCCAGAC CCTGGAGGGC AGCCGCTGCC CCTGGAAGGC ATGCCGACGT 
CAGCTTCTCC CGGGAGGCGG AATTCATGCG GGCCCTAGTC TGAGAAAGGC ACCCGCAGGG 
TGGACAGCAT GCTGCCCGCC AGCAGTGTGG CTGGATCCCC GAGTCCAGCC AGCCTTTCCG 
TTAGCCAGCC AGCCAGCGAG CGCTCCTAAA CCCTGGCCGC CGCCGGTTAT AAATGATTCA 
TGCAGCCCCG CCTCCCTCGG CGGCCACTTG ATGTTCCCTG CGCTCCAGCC CGGCGGTCCA 
CCGCCTCTCC TGCCGCTGGG CAGTGCCGCC GCTGGGCAGT GCCCTCCCCG CACCGCCGCC 
CCTCTGGGTG CTGCGTCCCT CCGCCCTCCC CGCACGCTGG TGCCGCAGCA TCTCTTGCCA 
CCATTCCATG CGCCCCTACC GATTGACATG CGACACCAGG AAGGAAGGTA CCATTACGAG 
CCTCATTCTG TCCACGGTGT GCACGGGCCC CCTGCCCTCA GCGGCAGCCC TGTCATCTCT 
GACATCTCCT TGATCCGGCT TTCCCCGCAC CCGGCTGGCC CTGGGGAGTC CCCCTTCAAC 
GCCCCCCACC CGTACGTGAA CCCCCACATG GAGCACTACC TCCGTTCTGT GCACAGCAGC 
CCCACGCTCT CCATGATCTC TGCAGCCAGG GGCCTCAGCC CCGCTGATGT GGCCCAGGAG 
CACCTTAAGG AGAGGGGACT GTTTGGCCTT CCTGCTCCAG GCACCACCCC CTCAGACTAT 
TACCACCAGA TGACCCTCGT GGCAGGCCAC CCCGCGCCCT ACGGGGACCT GCTGATGCAG 
AGCGGGGGCG CTGCCAGCGC ACCCCATCTC CACGACTACC TCAACCCCGT GGACGTGTCC 
CGTTTCTCCA GCCCGCGGGT GACGCCCCGC CTGAGCCGCA AGCGGGCGCT GTCCATCTCC 
CCACTCTCAG ACGCCAGCCT GGACCTGCAG CGGATGATCC GCACCTCACC CAACTCGCTA 
GTGGCCTACA TCAACAACTC CCGAAGCAGC TCGGCGGCCA GCGGTTCCTA CGGGCATCTG 
TCAGCGGGTG CCCTCAGCCC AGCCTTCACC TTCCCCCACC CCATCAACCC CGTGGCCTAC 
CAGCAGATTC TGAGCCAGCA GAGGGGTCTG GGGTCAGCCT TTGGACACAC ACCACCCCTG 
ATCCAGCCCT CACCCACCTT CCTGGCCCAG CAGCCCATGG CCCTCACCTC CATCAATGCC 
ACGCCCACCC AGCTCAGCAG CAGCAGCAAC TGTCTGAGTG ACACCAACCA GAACAAGCAG 
AGCAGTGAGT CGGCCGTCAG CAGCACCGTC AACCCTGTCG CCATTCACAA GCGCAGCAAG 
GTCAAGACCG AGCCTGAGGG CCTGCGGCCG GCCTCCCCTC TGGCGCTGAC GCAGGGCCAG 
GTGTCTGGAC ACGGCTCATG TGGGTGTGCC CTTCCCCTCT CCCAGGAGCA GCTGGCTGAC 
CTCAAGGAAG ATCTGGACAG GGATGACTGT AAGCAGGAGG CTGAGGTGGT CATCTATGAG 
ACCAACTGCC ACTGGGAAGA CTGCACCAAG GAGTACGACA CCCAGGAGCA GCTGGTGCAT 
CACATCAACA ACGAGCACAT CCACGGGGAG AAGAAGGAGT TTGTGTGCCG CTGGCAGGCC 
TGCACGCGGG AGCAGAAGCC CTTCAAGGCG CAGTACATGC TGGTGGTGCA CATGCGGCGA 
CACACGGGCG AGAAGCCCCA CAAGTGCACG TTCGAGGGCT GCTCGAAGGC CTACTCCCGC 
CTGGAGAACC TGAAGACACA CCTGCGGTCC CACACCGGGG AGAAGCCATA TGTGTGTGAG 
CACGAGGGCT GCAACAAAGC CTTCTCCAAC GCCTCGGACC GCGCCAAGCA CCAGAATCGC 
ACCCACTCCA ACGAGAAACC CTACATCTGC AAGATCCCAG GCTGCACCAA GAGATACACA 
GACCCCAGCT CTCTCCGGAA GCATGTGAAA ACGGTCCACG GCCCAGATGC CCACGTCACC 
AAGAAGCAGC GCAATGACGT GCACCTCCGC ACACCGCTGC TCAAAGAGAA TGGGGACAGT 
GAGGCCGGCA CGGAGCCTGG CGGCCCAGAG AGCACCGAGG CCAGCAGCAC CAGCCAGGCC 
GTGGAGGACT GCCTGCACGT CAGAGCCATC AAGACCGAGA GCTCCGGGCT GTGTCAGTCC 
AGCCCCGGGG CCCAGTCGTC CTGCAGCAGC GAGCCCTCTC CTCTGGGCAG TGCCCCCAAC 
AATGACAGTG GCGTGGAGAT GCCGGGGACG GGGCCCGGGA GCCTGGGAGA CCTGACGGCA 
CTGGATGACA CACCCCCAGG GGCCGACACC TCAGCCCTGG CTGCCCCCTC CGCTGGTGGC 
CTCCAGCTGC GCAAACACAT GACCACCATG CACCGGTTCG AGCAGCTCAA GAAGGAGAAG 
CTCAAGTCAC TCAAGGATTC CTGCTCATGG GCCGGGCCGA CTCCACACAC GCGGAACACC 
AAGCTGCCTC CCCTCCCGGG AAGTGGCTCC ATCCTGGAAA ACTTCAGTGG CAGTGGGGGC 
GGCGGGCCCG CGGGGCTGCT GCCGAACCCG CGGCTGTCGG AGCTGTCCGC GAGCGAGGTG 
ACCATGCTGA GCCAGCTGCA GGAGCGCCGC GACAGCTCCA CCAGCACGGT CAGCTCGGCC 
TACACCGTGA GCCGCCGCTC CTCCGGCATC TCCCCCTACT TCTCCAGCCG CCGCTCCAGC 
GAGGCCTCGC CCCTGGGCGC CGGCCGCCCG CACAACGCGA GCTCCGCTGA CTCCTACGAC 
CCCATCTCCA CGGACGCGTC GCGGCGCTCG AGCGAGGCCA GCCAGTGCAG CGGCGGCTCC 
GGGCTGCTCA ACCTCACGCC GGCGCAGCAG TACAGCCTGC GGGCCAAGTA CGCGGCAGCC 
ACTGGCGGCC CCCCGCCCAC TCCGCTGCCG GGCCTGGAGC GCATGAGCCT GCGGACCAGG 
CTGGCGCTGC TGGACGCGCC CGAGCGCACG CTGCCCGCCG GCTGCCCACG CCCACTGGGG 
CCGCGGCGTG GCAGCGACGG GCCGACCTAT GGCCACGGCC ACGCGGGGGC TGCGCCCGCC 
TTCCCCCACG AGGCTCCAGG CGGCGGAGCC AGGCGGGCCA GCGACCCTGT GCGGCGGCCC 
GATGCCCTGT CCCTGCCGCG GGTGCAGCGC TTCCACAGCA CCCACAACGT GAACCCCGGC 
CCGCTGCCGC CCTGTGCCGA CAGGCGAGGC CTCCGCCTGC AGAGCCACCC GAGCACCGAC 
GGCGGCCTGG CCCGCGGCGC CTACTCGCCC CGGCCGCCTA GCATCAGCGA GAACGTGGCG 
ATGGAGGCCG TGGCGGCAGG AGTGGACGGC GCGGGGCCCG AGGCCGACCT GGGGCTGCCG 
GAGGACGACC TGGTGCTTCC AGACGACGTG GTGCAGTACA TCAAGGCGCA CGCCAGTGGC 
GCTCTGGACG AGGGCACCGG GCAGGTGTAT CCCACGGAAA GCACTGGCTT CTCTGACAAC 
CCCAGACTAC CCAGCCCGGG GCTGCACGGC CAGCGCAGGA TGGTGGCTGC GGACTCCAAC 
GTGGGCCCCT CCGCCCCTAT GCTGGGAGGA TGCCAGTTAG GCTTTGGGGC GCCCTCCAGC 
CTGAACAAAA ATAACATGCC TGTGCAGTGG AATGAGGTGA GCTCCGGCAC CGTAGACGCC 
CTGGCCAGCC AGGTGAAGCC TCCACCCTTT CCTCAGGGCA ACCTGGCGGT GGTGCAGCAG 
AAGCCTGCCT TTGGCCAGTA CCCGGGCTAC AGTCCGCAAG GCCTACAGGC TAGCCCTGGG 
GGCCTGGACA GCACGCAGCC ACACCTGCAG CCCCGCAGCG GAGCCCCCTC CCAGGGCATC 
CCCAGGGTAA ACTACATGCA GCAGCTGCGA CAGCCAGTGG CAGGCAGCCA GTGTCCTGGC 
ATGACTACCA CTATGAGCCC CCATGCCTGC TATGGCCAAG TCCACCCCCA GCTGAGCCCC 
AGCACCATCA GTGGGGCCCT CAACCAGTTC CCCCAATCCT GCAGCAACAT GCCAGCCAAG 
CCAGGGCATC TGGGGCACCC TCAGCAGACA GAAGTGGCAC CTGACCCCAC CACGATGGGC 
AATCGCCACA GGGAACTTGG GGTCCCCGAT TCAGCCCTGG CTGGAGTGCC ACCACCTCAC 
CCAGTCCAGA GCTACCCACA GCAGAGCCAT CACCTGGCAG CCTCCATGAG CCAGGAGGGC 
TACCACCAGG TCCCCAGCCT TCTGCCTGCC CGCCAGCCTG GCTTCATGGA GCCCCAAACA 
GGCCCGATGG GGGTGGCTAC AGCAGGCTTT GGCCTAGTGC AGCCCCGGCC TCCCCTCGAG 
CCCAGCCCCA CTGGCCGCCA CCGTGGGGTA CGTGCTGTGC AGCAGCAGCT GGCCTACGCC 
AGGGCCACAG GCCATGCCAT GGCTGCCATG CCGTCCAGTC AGGAAACAGC AGAGGCTGTG 
CCCAAGGGAG CGATGGGCAA CATGGGGTCG GTGCCTCCCC AGCCGCCTCC GCAGGACGCA 
GGTGGGGCCC CGGACCACAG CATGCTCTAC TACTACGGCC AGATCCACAT GTACGAACAG 
GATGGAGGCC TGGAGAACCT CGGGAGCTGC CAGGTCATGC GGTCCCAGCC ACCACAGCCA 
CAGGCCTGTC AGGACAGCAT CCAGCCCCAG CCCTTGCCCT CACCAGGGGT CAACCAGGTG 
TCCAGCACTG TGGACTCCCA GCTCCTGGAG GCCCCCCAGA TTGACTTCGA TGCCATCATG 
GATGATGGCG ATCACTCGAG TTTGTTCTCG GGTGCTCTGA GCCCCAGCCT CCTCCACAGC 
CTCTCCCAGA ACTCCTCCCG CCTCACCACC CCCCGAAACT CCTTGACCCT GCCCTCCATC 
CCCGCAGGCA TCAGCAACAT GGCTGTCGGG GACATGAGCT CCATGCTCAC CAGCCTCGCC 
GAGGAGAGCA AGTTCCTGAA CATGATGACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( XM_017003818.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859307.1 Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X2, mRNA.

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CloneID	OHu89280
Clone ID Related Accession (Same CDS sequence)	XM_017003818.1
Accession Version	XM_017003818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	zinc finger protein GLI2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCCA AGTTCCAGAC CCTGGAGGGC AGCCGCTGCC CCTGGAAGGC ATGCCGACGT 
CAGCTTCTCC CGGGAGGCGG AATTCATGCG GGCCCTAGTC TGAGAAAGGC ACCCGCAGGG 
TGGACAGCAT GCTGCCCGCC AGCAGTGTGG CTGGATCCCC GAGTCCAGCC AGCCTTTCCG 
TTAGCCAGCC AGCCAGCGAG CGCTCCTAAA CCCTGGCCGC CGCCGGTTAT AAATGATTCA 
TGCAGCCCCG CCTCCCTCGG CGGCCACTTG ATGTTCCCTG CGCTCCAGCC CGGCGGTCCA 
CCGCCTCTCC TGCCGCTGGG CAGTGCCGCC GCTGGGCAGT GCCCTCCCCG CACCGCCGCC 
CCTCTGGGTG CTGCGTCCCT CCGCCCTCCC CGCACGCTGG TGCCGCAGCA TCTCTTGCCA 
CCATTCCATG CGCCCCTACC GATTGACATG CGACACCAGG AAGGAAGGTA CCATTACGAG 
CCTCATTCTG TCCACGGTGT GCACGGGCCC CCTGCCCTCA GCGGCAGCCC TGTCATCTCT 
GACATCTCCT TGATCCGGCT TTCCCCGCAC CCGGCTGGCC CTGGGGAGTC CCCCTTCAAC 
GCCCCCCACC CGTACGTGAA CCCCCACATG GAGCACTACC TCCGTTCTGT GCACAGCAGC 
CCCACGCTCT CCATGATCTC TGCAGCCAGG GGCCTCAGCC CCGCTGATGT GGCCCAGGAG 
CACCTTAAGG AGAGGGGACT GTTTGGCCTT CCTGCTCCAG GCACCACCCC CTCAGACTAT 
TACCACCAGA TGACCCTCGT GGCAGGCCAC CCCGCGCCCT ACGGGGACCT GCTGATGCAG 
AGCGGGGGCG CTGCCAGCGC ACCCCATCTC CACGACTACC TCAACCCCGT GGACGTGTCC 
CGTTTCTCCA GCCCGCGGGT GACGCCCCGC CTGAGCCGCA AGCGGGCGCT GTCCATCTCC 
CCACTCTCAG ACGCCAGCCT GGACCTGCAG CGGATGATCC GCACCTCACC CAACTCGCTA 
GTGGCCTACA TCAACAACTC CCGAAGCAGC TCGGCGGCCA GCGGTTCCTA CGGGCATCTG 
TCAGCGGGTG CCCTCAGCCC AGCCTTCACC TTCCCCCACC CCATCAACCC CGTGGCCTAC 
CAGCAGATTC TGAGCCAGCA GAGGGGTCTG GGGTCAGCCT TTGGACACAC ACCACCCCTG 
ATCCAGCCCT CACCCACCTT CCTGGCCCAG CAGCCCATGG CCCTCACCTC CATCAATGCC 
ACGCCCACCC AGCTCAGCAG CAGCAGCAAC TGTCTGAGTG ACACCAACCA GAACAAGCAG 
AGCAGTGAGT CGGCCGTCAG CAGCACCGTC AACCCTGTCG CCATTCACAA GCGCAGCAAG 
GTCAAGACCG AGCCTGAGGG CCTGCGGCCG GCCTCCCCTC TGGCGCTGAC GCAGGAGCAG 
CTGGCTGACC TCAAGGAAGA TCTGGACAGG GATGACTGTA AGCAGGAGGC TGAGGTGGTC 
ATCTATGAGA CCAACTGCCA CTGGGAAGAC TGCACCAAGG AGTACGACAC CCAGGAGCAG 
CTGGTGCATC ACATCAACAA CGAGCACATC CACGGGGAGA AGAAGGAGTT TGTGTGCCGC 
TGGCAGGCCT GCACGCGGGA GCAGAAGCCC TTCAAGGCGC AGTACATGCT GGTGGTGCAC 
ATGCGGCGAC ACACGGGCGA GAAGCCCCAC AAGTGCACGT TCGAGGGCTG CTCGAAGGCC 
TACTCCCGCC TGGAGAACCT GAAGACACAC CTGCGGTCCC ACACCGGGGA GAAGCCATAT 
GTGTGTGAGC ACGAGGGCTG CAACAAAGCC TTCTCCAACG CCTCGGACCG CGCCAAGCAC 
CAGAATCGCA CCCACTCCAA CGAGAAACCC TACATCTGCA AGATCCCAGG CTGCACCAAG 
AGATACACAG ACCCCAGCTC TCTCCGGAAG CATGTGAAAA CGGTCCACGG CCCAGATGCC 
CACGTCACCA AGAAGCAGCG CAATGACGTG CACCTCCGCA CACCGCTGCT CAAAGAGAAT 
GGGGACAGTG AGGCCGGCAC GGAGCCTGGC GGCCCAGAGA GCACCGAGGC CAGCAGCACC 
AGCCAGGCCG TGGAGGACTG CCTGCACGTC AGAGCCATCA AGACCGAGAG CTCCGGGCTG 
TGTCAGTCCA GCCCCGGGGC CCAGTCGTCC TGCAGCAGCG AGCCCTCTCC TCTGGGCAGT 
GCCCCCAACA ATGACAGTGG CGTGGAGATG CCGGGGACGG GGCCCGGGAG CCTGGGAGAC 
CTGACGGCAC TGGATGACAC ACCCCCAGGG GCCGACACCT CAGCCCTGGC TGCCCCCTCC 
GCTGGTGGCC TCCAGCTGCG CAAACACATG ACCACCATGC ACCGGTTCGA GCAGCTCAAG 
AAGGAGAAGC TCAAGTCACT CAAGGATTCC TGCTCATGGG CCGGGCCGAC TCCACACACG 
CGGAACACCA AGCTGCCTCC CCTCCCGGGA AGTGGCTCCA TCCTGGAAAA CTTCAGTGGC 
AGTGGGGGCG GCGGGCCCGC GGGGCTGCTG CCGAACCCGC GGCTGTCGGA GCTGTCCGCG 
AGCGAGGTGA CCATGCTGAG CCAGCTGCAG GAGCGCCGCG ACAGCTCCAC CAGCACGGTC 
AGCTCGGCCT ACACCGTGAG CCGCCGCTCC TCCGGCATCT CCCCCTACTT CTCCAGCCGC 
CGCTCCAGCG AGGCCTCGCC CCTGGGCGCC GGCCGCCCGC ACAACGCGAG CTCCGCTGAC 
TCCTACGACC CCATCTCCAC GGACGCGTCG CGGCGCTCGA GCGAGGCCAG CCAGTGCAGC 
GGCGGCTCCG GGCTGCTCAA CCTCACGCCG GCGCAGCAGT ACAGCCTGCG GGCCAAGTAC 
GCGGCAGCCA CTGGCGGCCC CCCGCCCACT CCGCTGCCGG GCCTGGAGCG CATGAGCCTG 
CGGACCAGGC TGGCGCTGCT GGACGCGCCC GAGCGCACGC TGCCCGCCGG CTGCCCACGC 
CCACTGGGGC CGCGGCGTGG CAGCGACGGG CCGACCTATG GCCACGGCCA CGCGGGGGCT 
GCGCCCGCCT TCCCCCACGA GGCTCCAGGC GGCGGAGCCA GGCGGGCCAG CGACCCTGTG 
CGGCGGCCCG ATGCCCTGTC CCTGCCGCGG GTGCAGCGCT TCCACAGCAC CCACAACGTG 
AACCCCGGCC CGCTGCCGCC CTGTGCCGAC AGGCGAGGCC TCCGCCTGCA GAGCCACCCG 
AGCACCGACG GCGGCCTGGC CCGCGGCGCC TACTCGCCCC GGCCGCCTAG CATCAGCGAG 
AACGTGGCGA TGGAGGCCGT GGCGGCAGGA GTGGACGGCG CGGGGCCCGA GGCCGACCTG 
GGGCTGCCGG AGGACGACCT GGTGCTTCCA GACGACGTGG TGCAGTACAT CAAGGCGCAC 
GCCAGTGGCG CTCTGGACGA GGGCACCGGG CAGGTGTATC CCACGGAAAG CACTGGCTTC 
TCTGACAACC CCAGACTACC CAGCCCGGGG CTGCACGGCC AGCGCAGGAT GGTGGCTGCG 
GACTCCAACG TGGGCCCCTC CGCCCCTATG CTGGGAGGAT GCCAGTTAGG CTTTGGGGCG 
CCCTCCAGCC TGAACAAAAA TAACATGCCT GTGCAGTGGA ATGAGGTGAG CTCCGGCACC 
GTAGACGCCC TGGCCAGCCA GGTGAAGCCT CCACCCTTTC CTCAGGGCAA CCTGGCGGTG 
GTGCAGCAGA AGCCTGCCTT TGGCCAGTAC CCGGGCTACA GTCCGCAAGG CCTACAGGCT 
AGCCCTGGGG GCCTGGACAG CACGCAGCCA CACCTGCAGC CCCGCAGCGG AGCCCCCTCC 
CAGGGCATCC CCAGGGTAAA CTACATGCAG CAGCTGCGAC AGCCAGTGGC AGGCAGCCAG 
TGTCCTGGCA TGACTACCAC TATGAGCCCC CATGCCTGCT ATGGCCAAGT CCACCCCCAG 
CTGAGCCCCA GCACCATCAG TGGGGCCCTC AACCAGTTCC CCCAATCCTG CAGCAACATG 
CCAGCCAAGC CAGGGCATCT GGGGCACCCT CAGCAGACAG AAGTGGCACC TGACCCCACC 
ACGATGGGCA ATCGCCACAG GGAACTTGGG GTCCCCGATT CAGCCCTGGC TGGAGTGCCA 
CCACCTCACC CAGTCCAGAG CTACCCACAG CAGAGCCATC ACCTGGCAGC CTCCATGAGC 
CAGGAGGGCT ACCACCAGGT CCCCAGCCTT CTGCCTGCCC GCCAGCCTGG CTTCATGGAG 
CCCCAAACAG GCCCGATGGG GGTGGCTACA GCAGGCTTTG GCCTAGTGCA GCCCCGGCCT 
CCCCTCGAGC CCAGCCCCAC TGGCCGCCAC CGTGGGGTAC GTGCTGTGCA GCAGCAGCTG 
GCCTACGCCA GGGCCACAGG CCATGCCATG GCTGCCATGC CGTCCAGTCA GGAAACAGCA 
GAGGCTGTGC CCAAGGGAGC GATGGGCAAC ATGGGGTCGG TGCCTCCCCA GCCGCCTCCG 
CAGGACGCAG GTGGGGCCCC GGACCACAGC ATGCTCTACT ACTACGGCCA GATCCACATG 
TACGAACAGG ATGGAGGCCT GGAGAACCTC GGGAGCTGCC AGGTCATGCG GTCCCAGCCA 
CCACAGCCAC AGGCCTGTCA GGACAGCATC CAGCCCCAGC CCTTGCCCTC ACCAGGGGTC 
AACCAGGTGT CCAGCACTGT GGACTCCCAG CTCCTGGAGG CCCCCCAGAT TGACTTCGAT 
GCCATCATGG ATGATGGCGA TCACTCGAGT TTGTTCTCGG GTGCTCTGAG CCCCAGCCTC 
CTCCACAGCC TCTCCCAGAA CTCCTCCCGC CTCACCACCC CCCGAAACTC CTTGACCCTG 
CCCTCCATCC CCGCAGGCAT CAGCAACATG GCTGTCGGGG ACATGAGCTC CATGCTCACC 
AGCCTCGCCG AGGAGAGCAA GTTCCTGAAC ATGATGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859307.1
CDS	411..5372
Translation	MTPKFQTLEGSRCPWKACRRQLLPGGGIHAGPSLRKAPAGWTACCPPAVWLDPRVQPAFPLASQPASAPKPWPPPVINDSCSPASLGGHLMFPALQPGGPPPLLPLGSAAAGQCPPRTAAPLGAASLRPPRTLVPQHLLPPFHAPLPIDMRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	XM_017003818.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003818.1

ORF Insert Sequence:

ATGACCCCCA AGTTCCAGAC CCTGGAGGGC AGCCGCTGCC CCTGGAAGGC ATGCCGACGT 
CAGCTTCTCC CGGGAGGCGG AATTCATGCG GGCCCTAGTC TGAGAAAGGC ACCCGCAGGG 
TGGACAGCAT GCTGCCCGCC AGCAGTGTGG CTGGATCCCC GAGTCCAGCC AGCCTTTCCG 
TTAGCCAGCC AGCCAGCGAG CGCTCCTAAA CCCTGGCCGC CGCCGGTTAT AAATGATTCA 
TGCAGCCCCG CCTCCCTCGG CGGCCACTTG ATGTTCCCTG CGCTCCAGCC CGGCGGTCCA 
CCGCCTCTCC TGCCGCTGGG CAGTGCCGCC GCTGGGCAGT GCCCTCCCCG CACCGCCGCC 
CCTCTGGGTG CTGCGTCCCT CCGCCCTCCC CGCACGCTGG TGCCGCAGCA TCTCTTGCCA 
CCATTCCATG CGCCCCTACC GATTGACATG CGACACCAGG AAGGAAGGTA CCATTACGAG 
CCTCATTCTG TCCACGGTGT GCACGGGCCC CCTGCCCTCA GCGGCAGCCC TGTCATCTCT 
GACATCTCCT TGATCCGGCT TTCCCCGCAC CCGGCTGGCC CTGGGGAGTC CCCCTTCAAC 
GCCCCCCACC CGTACGTGAA CCCCCACATG GAGCACTACC TCCGTTCTGT GCACAGCAGC 
CCCACGCTCT CCATGATCTC TGCAGCCAGG GGCCTCAGCC CCGCTGATGT GGCCCAGGAG 
CACCTTAAGG AGAGGGGACT GTTTGGCCTT CCTGCTCCAG GCACCACCCC CTCAGACTAT 
TACCACCAGA TGACCCTCGT GGCAGGCCAC CCCGCGCCCT ACGGGGACCT GCTGATGCAG 
AGCGGGGGCG CTGCCAGCGC ACCCCATCTC CACGACTACC TCAACCCCGT GGACGTGTCC 
CGTTTCTCCA GCCCGCGGGT GACGCCCCGC CTGAGCCGCA AGCGGGCGCT GTCCATCTCC 
CCACTCTCAG ACGCCAGCCT GGACCTGCAG CGGATGATCC GCACCTCACC CAACTCGCTA 
GTGGCCTACA TCAACAACTC CCGAAGCAGC TCGGCGGCCA GCGGTTCCTA CGGGCATCTG 
TCAGCGGGTG CCCTCAGCCC AGCCTTCACC TTCCCCCACC CCATCAACCC CGTGGCCTAC 
CAGCAGATTC TGAGCCAGCA GAGGGGTCTG GGGTCAGCCT TTGGACACAC ACCACCCCTG 
ATCCAGCCCT CACCCACCTT CCTGGCCCAG CAGCCCATGG CCCTCACCTC CATCAATGCC 
ACGCCCACCC AGCTCAGCAG CAGCAGCAAC TGTCTGAGTG ACACCAACCA GAACAAGCAG 
AGCAGTGAGT CGGCCGTCAG CAGCACCGTC AACCCTGTCG CCATTCACAA GCGCAGCAAG 
GTCAAGACCG AGCCTGAGGG CCTGCGGCCG GCCTCCCCTC TGGCGCTGAC GCAGGAGCAG 
CTGGCTGACC TCAAGGAAGA TCTGGACAGG GATGACTGTA AGCAGGAGGC TGAGGTGGTC 
ATCTATGAGA CCAACTGCCA CTGGGAAGAC TGCACCAAGG AGTACGACAC CCAGGAGCAG 
CTGGTGCATC ACATCAACAA CGAGCACATC CACGGGGAGA AGAAGGAGTT TGTGTGCCGC 
TGGCAGGCCT GCACGCGGGA GCAGAAGCCC TTCAAGGCGC AGTACATGCT GGTGGTGCAC 
ATGCGGCGAC ACACGGGCGA GAAGCCCCAC AAGTGCACGT TCGAGGGCTG CTCGAAGGCC 
TACTCCCGCC TGGAGAACCT GAAGACACAC CTGCGGTCCC ACACCGGGGA GAAGCCATAT 
GTGTGTGAGC ACGAGGGCTG CAACAAAGCC TTCTCCAACG CCTCGGACCG CGCCAAGCAC 
CAGAATCGCA CCCACTCCAA CGAGAAACCC TACATCTGCA AGATCCCAGG CTGCACCAAG 
AGATACACAG ACCCCAGCTC TCTCCGGAAG CATGTGAAAA CGGTCCACGG CCCAGATGCC 
CACGTCACCA AGAAGCAGCG CAATGACGTG CACCTCCGCA CACCGCTGCT CAAAGAGAAT 
GGGGACAGTG AGGCCGGCAC GGAGCCTGGC GGCCCAGAGA GCACCGAGGC CAGCAGCACC 
AGCCAGGCCG TGGAGGACTG CCTGCACGTC AGAGCCATCA AGACCGAGAG CTCCGGGCTG 
TGTCAGTCCA GCCCCGGGGC CCAGTCGTCC TGCAGCAGCG AGCCCTCTCC TCTGGGCAGT 
GCCCCCAACA ATGACAGTGG CGTGGAGATG CCGGGGACGG GGCCCGGGAG CCTGGGAGAC 
CTGACGGCAC TGGATGACAC ACCCCCAGGG GCCGACACCT CAGCCCTGGC TGCCCCCTCC 
GCTGGTGGCC TCCAGCTGCG CAAACACATG ACCACCATGC ACCGGTTCGA GCAGCTCAAG 
AAGGAGAAGC TCAAGTCACT CAAGGATTCC TGCTCATGGG CCGGGCCGAC TCCACACACG 
CGGAACACCA AGCTGCCTCC CCTCCCGGGA AGTGGCTCCA TCCTGGAAAA CTTCAGTGGC 
AGTGGGGGCG GCGGGCCCGC GGGGCTGCTG CCGAACCCGC GGCTGTCGGA GCTGTCCGCG 
AGCGAGGTGA CCATGCTGAG CCAGCTGCAG GAGCGCCGCG ACAGCTCCAC CAGCACGGTC 
AGCTCGGCCT ACACCGTGAG CCGCCGCTCC TCCGGCATCT CCCCCTACTT CTCCAGCCGC 
CGCTCCAGCG AGGCCTCGCC CCTGGGCGCC GGCCGCCCGC ACAACGCGAG CTCCGCTGAC 
TCCTACGACC CCATCTCCAC GGACGCGTCG CGGCGCTCGA GCGAGGCCAG CCAGTGCAGC 
GGCGGCTCCG GGCTGCTCAA CCTCACGCCG GCGCAGCAGT ACAGCCTGCG GGCCAAGTAC 
GCGGCAGCCA CTGGCGGCCC CCCGCCCACT CCGCTGCCGG GCCTGGAGCG CATGAGCCTG 
CGGACCAGGC TGGCGCTGCT GGACGCGCCC GAGCGCACGC TGCCCGCCGG CTGCCCACGC 
CCACTGGGGC CGCGGCGTGG CAGCGACGGG CCGACCTATG GCCACGGCCA CGCGGGGGCT 
GCGCCCGCCT TCCCCCACGA GGCTCCAGGC GGCGGAGCCA GGCGGGCCAG CGACCCTGTG 
CGGCGGCCCG ATGCCCTGTC CCTGCCGCGG GTGCAGCGCT TCCACAGCAC CCACAACGTG 
AACCCCGGCC CGCTGCCGCC CTGTGCCGAC AGGCGAGGCC TCCGCCTGCA GAGCCACCCG 
AGCACCGACG GCGGCCTGGC CCGCGGCGCC TACTCGCCCC GGCCGCCTAG CATCAGCGAG 
AACGTGGCGA TGGAGGCCGT GGCGGCAGGA GTGGACGGCG CGGGGCCCGA GGCCGACCTG 
GGGCTGCCGG AGGACGACCT GGTGCTTCCA GACGACGTGG TGCAGTACAT CAAGGCGCAC 
GCCAGTGGCG CTCTGGACGA GGGCACCGGG CAGGTGTATC CCACGGAAAG CACTGGCTTC 
TCTGACAACC CCAGACTACC CAGCCCGGGG CTGCACGGCC AGCGCAGGAT GGTGGCTGCG 
GACTCCAACG TGGGCCCCTC CGCCCCTATG CTGGGAGGAT GCCAGTTAGG CTTTGGGGCG 
CCCTCCAGCC TGAACAAAAA TAACATGCCT GTGCAGTGGA ATGAGGTGAG CTCCGGCACC 
GTAGACGCCC TGGCCAGCCA GGTGAAGCCT CCACCCTTTC CTCAGGGCAA CCTGGCGGTG 
GTGCAGCAGA AGCCTGCCTT TGGCCAGTAC CCGGGCTACA GTCCGCAAGG CCTACAGGCT 
AGCCCTGGGG GCCTGGACAG CACGCAGCCA CACCTGCAGC CCCGCAGCGG AGCCCCCTCC 
CAGGGCATCC CCAGGGTAAA CTACATGCAG CAGCTGCGAC AGCCAGTGGC AGGCAGCCAG 
TGTCCTGGCA TGACTACCAC TATGAGCCCC CATGCCTGCT ATGGCCAAGT CCACCCCCAG 
CTGAGCCCCA GCACCATCAG TGGGGCCCTC AACCAGTTCC CCCAATCCTG CAGCAACATG 
CCAGCCAAGC CAGGGCATCT GGGGCACCCT CAGCAGACAG AAGTGGCACC TGACCCCACC 
ACGATGGGCA ATCGCCACAG GGAACTTGGG GTCCCCGATT CAGCCCTGGC TGGAGTGCCA 
CCACCTCACC CAGTCCAGAG CTACCCACAG CAGAGCCATC ACCTGGCAGC CTCCATGAGC 
CAGGAGGGCT ACCACCAGGT CCCCAGCCTT CTGCCTGCCC GCCAGCCTGG CTTCATGGAG 
CCCCAAACAG GCCCGATGGG GGTGGCTACA GCAGGCTTTG GCCTAGTGCA GCCCCGGCCT 
CCCCTCGAGC CCAGCCCCAC TGGCCGCCAC CGTGGGGTAC GTGCTGTGCA GCAGCAGCTG 
GCCTACGCCA GGGCCACAGG CCATGCCATG GCTGCCATGC CGTCCAGTCA GGAAACAGCA 
GAGGCTGTGC CCAAGGGAGC GATGGGCAAC ATGGGGTCGG TGCCTCCCCA GCCGCCTCCG 
CAGGACGCAG GTGGGGCCCC GGACCACAGC ATGCTCTACT ACTACGGCCA GATCCACATG 
TACGAACAGG ATGGAGGCCT GGAGAACCTC GGGAGCTGCC AGGTCATGCG GTCCCAGCCA 
CCACAGCCAC AGGCCTGTCA GGACAGCATC CAGCCCCAGC CCTTGCCCTC ACCAGGGGTC 
AACCAGGTGT CCAGCACTGT GGACTCCCAG CTCCTGGAGG CCCCCCAGAT TGACTTCGAT 
GCCATCATGG ATGATGGCGA TCACTCGAGT TTGTTCTCGG GTGCTCTGAG CCCCAGCCTC 
CTCCACAGCC TCTCCCAGAA CTCCTCCCGC CTCACCACCC CCCGAAACTC CTTGACCCTG 
CCCTCCATCC CCGCAGGCAT CAGCAACATG GCTGTCGGGG ACATGAGCTC CATGCTCACC 
AGCCTCGCCG AGGAGAGCAA GTTCCTGAAC ATGATGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( XM_011510972.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509274.2 Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X6, mRNA.

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CloneID	OHu89281
Clone ID Related Accession (Same CDS sequence)	XM_011510972.2
Accession Version	XM_011510972.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	zinc finger protein GLI2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAGC CGTGGTTCAC ACTCAGGTCT GTCTGGATTC ATGGCTCAGT GCCGCAGCAT 
CTCTTGCCAC CATTCCATGC GCCCCTACCG ATTGACATGC GACACCAGGA AGGAAGGTAC 
CATTACGAGC CTCATTCTGT CCACGGTGTG CACGGGCCCC CTGCCCTCAG CGGCAGCCCT 
GTCATCTCTG ACATCTCCTT GATCCGGCTT TCCCCGCACC CGGCTGGCCC TGGGGAGTCC 
CCCTTCAACG CCCCCCACCC GTACGTGAAC CCCCACATGG AGCACTACCT CCGTTCTGTG 
CACAGCAGCC CCACGCTCTC CATGATCTCT GCAGCCAGGG GCCTCAGCCC CGCTGATGTG 
GCCCAGGAGC ACCTTAAGGA GAGGGGACTG TTTGGCCTTC CTGCTCCAGG CACCACCCCC 
TCAGACTATT ACCACCAGAT GACCCTCGTG GCAGGCCACC CCGCGCCCTA CGGGGACCTG 
CTGATGCAGA GCGGGGGCGC TGCCAGCGCA CCCCATCTCC ACGACTACCT CAACCCCGTG 
GACGTGTCCC GTTTCTCCAG CCCGCGGGTG ACGCCCCGCC TGAGCCGCAA GCGGGCGCTG 
TCCATCTCCC CACTCTCAGA CGCCAGCCTG GACCTGCAGC GGATGATCCG CACCTCACCC 
AACTCGCTAG TGGCCTACAT CAACAACTCC CGAAGCAGCT CGGCGGCCAG CGGTTCCTAC 
GGGCATCTGT CAGCGGGTGC CCTCAGCCCA GCCTTCACCT TCCCCCACCC CATCAACCCC 
GTGGCCTACC AGCAGATTCT GAGCCAGCAG AGGGGTCTGG GGTCAGCCTT TGGACACACA 
CCACCCCTGA TCCAGCCCTC ACCCACCTTC CTGGCCCAGC AGCCCATGGC CCTCACCTCC 
ATCAATGCCA CGCCCACCCA GCTCAGCAGC AGCAGCAACT GTCTGAGTGA CACCAACCAG 
AACAAGCAGA GCAGTGAGTC GGCCGTCAGC AGCACCGTCA ACCCTGTCGC CATTCACAAG 
CGCAGCAAGG TCAAGACCGA GCCTGAGGGC CTGCGGCCGG CCTCCCCTCT GGCGCTGACG 
CAGGGCCAGG TGTCTGGACA CGGCTCATGT GGGTGTGCCC TTCCCCTCTC CCAGGAGCAG 
CTGGCTGACC TCAAGGAAGA TCTGGACAGG GATGACTGTA AGCAGGAGGC TGAGGTGGTC 
ATCTATGAGA CCAACTGCCA CTGGGAAGAC TGCACCAAGG AGTACGACAC CCAGGAGCAG 
CTGGTGCATC ACATCAACAA CGAGCACATC CACGGGGAGA AGAAGGAGTT TGTGTGCCGC 
TGGCAGGCCT GCACGCGGGA GCAGAAGCCC TTCAAGGCGC AGTACATGCT GGTGGTGCAC 
ATGCGGCGAC ACACGGGCGA GAAGCCCCAC AAGTGCACGT TCGAGGGCTG CTCGAAGGCC 
TACTCCCGCC TGGAGAACCT GAAGACACAC CTGCGGTCCC ACACCGGGGA GAAGCCATAT 
GTGTGTGAGC ACGAGGGCTG CAACAAAGCC TTCTCCAACG CCTCGGACCG CGCCAAGCAC 
CAGAATCGCA CCCACTCCAA CGAGAAACCC TACATCTGCA AGATCCCAGG CTGCACCAAG 
AGATACACAG ACCCCAGCTC TCTCCGGAAG CATGTGAAAA CGGTCCACGG CCCAGATGCC 
CACGTCACCA AGAAGCAGCG CAATGACGTG CACCTCCGCA CACCGCTGCT CAAAGAGAAT 
GGGGACAGTG AGGCCGGCAC GGAGCCTGGC GGCCCAGAGA GCACCGAGGC CAGCAGCACC 
AGCCAGGCCG TGGAGGACTG CCTGCACGTC AGAGCCATCA AGACCGAGAG CTCCGGGCTG 
TGTCAGTCCA GCCCCGGGGC CCAGTCGTCC TGCAGCAGCG AGCCCTCTCC TCTGGGCAGT 
GCCCCCAACA ATGACAGTGG CGTGGAGATG CCGGGGACGG GGCCCGGGAG CCTGGGAGAC 
CTGACGGCAC TGGATGACAC ACCCCCAGGG GCCGACACCT CAGCCCTGGC TGCCCCCTCC 
GCTGGTGGCC TCCAGCTGCG CAAACACATG ACCACCATGC ACCGGTTCGA GCAGCTCAAG 
AAGGAGAAGC TCAAGTCACT CAAGGATTCC TGCTCATGGG CCGGGCCGAC TCCACACACG 
CGGAACACCA AGCTGCCTCC CCTCCCGGGA AGTGGCTCCA TCCTGGAAAA CTTCAGTGGC 
AGTGGGGGCG GCGGGCCCGC GGGGCTGCTG CCGAACCCGC GGCTGTCGGA GCTGTCCGCG 
AGCGAGGTGA CCATGCTGAG CCAGCTGCAG GAGCGCCGCG ACAGCTCCAC CAGCACGGTC 
AGCTCGGCCT ACACCGTGAG CCGCCGCTCC TCCGGCATCT CCCCCTACTT CTCCAGCCGC 
CGCTCCAGCG AGGCCTCGCC CCTGGGCGCC GGCCGCCCGC ACAACGCGAG CTCCGCTGAC 
TCCTACGACC CCATCTCCAC GGACGCGTCG CGGCGCTCGA GCGAGGCCAG CCAGTGCAGC 
GGCGGCTCCG GGCTGCTCAA CCTCACGCCG GCGCAGCAGT ACAGCCTGCG GGCCAAGTAC 
GCGGCAGCCA CTGGCGGCCC CCCGCCCACT CCGCTGCCGG GCCTGGAGCG CATGAGCCTG 
CGGACCAGGC TGGCGCTGCT GGACGCGCCC GAGCGCACGC TGCCCGCCGG CTGCCCACGC 
CCACTGGGGC CGCGGCGTGG CAGCGACGGG CCGACCTATG GCCACGGCCA CGCGGGGGCT 
GCGCCCGCCT TCCCCCACGA GGCTCCAGGC GGCGGAGCCA GGCGGGCCAG CGACCCTGTG 
CGGCGGCCCG ATGCCCTGTC CCTGCCGCGG GTGCAGCGCT TCCACAGCAC CCACAACGTG 
AACCCCGGCC CGCTGCCGCC CTGTGCCGAC AGGCGAGGCC TCCGCCTGCA GAGCCACCCG 
AGCACCGACG GCGGCCTGGC CCGCGGCGCC TACTCGCCCC GGCCGCCTAG CATCAGCGAG 
AACGTGGCGA TGGAGGCCGT GGCGGCAGGA GTGGACGGCG CGGGGCCCGA GGCCGACCTG 
GGGCTGCCGG AGGACGACCT GGTGCTTCCA GACGACGTGG TGCAGTACAT CAAGGCGCAC 
GCCAGTGGCG CTCTGGACGA GGGCACCGGG CAGGTGTATC CCACGGAAAG CACTGGCTTC 
TCTGACAACC CCAGACTACC CAGCCCGGGG CTGCACGGCC AGCGCAGGAT GGTGGCTGCG 
GACTCCAACG TGGGCCCCTC CGCCCCTATG CTGGGAGGAT GCCAGTTAGG CTTTGGGGCG 
CCCTCCAGCC TGAACAAAAA TAACATGCCT GTGCAGTGGA ATGAGGTGAG CTCCGGCACC 
GTAGACGCCC TGGCCAGCCA GGTGAAGCCT CCACCCTTTC CTCAGGGCAA CCTGGCGGTG 
GTGCAGCAGA AGCCTGCCTT TGGCCAGTAC CCGGGCTACA GTCCGCAAGG CCTACAGGCT 
AGCCCTGGGG GCCTGGACAG CACGCAGCCA CACCTGCAGC CCCGCAGCGG AGCCCCCTCC 
CAGGGCATCC CCAGGGTAAA CTACATGCAG CAGCTGCGAC AGCCAGTGGC AGGCAGCCAG 
TGTCCTGGCA TGACTACCAC TATGAGCCCC CATGCCTGCT ATGGCCAAGT CCACCCCCAG 
CTGAGCCCCA GCACCATCAG TGGGGCCCTC AACCAGTTCC CCCAATCCTG CAGCAACATG 
CCAGCCAAGC CAGGGCATCT GGGGCACCCT CAGCAGACAG AAGTGGCACC TGACCCCACC 
ACGATGGGCA ATCGCCACAG GGAACTTGGG GTCCCCGATT CAGCCCTGGC TGGAGTGCCA 
CCACCTCACC CAGTCCAGAG CTACCCACAG CAGAGCCATC ACCTGGCAGC CTCCATGAGC 
CAGGAGGGCT ACCACCAGGT CCCCAGCCTT CTGCCTGCCC GCCAGCCTGG CTTCATGGAG 
CCCCAAACAG GCCCGATGGG GGTGGCTACA GCAGGCTTTG GCCTAGTGCA GCCCCGGCCT 
CCCCTCGAGC CCAGCCCCAC TGGCCGCCAC CGTGGGGTAC GTGCTGTGCA GCAGCAGCTG 
GCCTACGCCA GGGCCACAGG CCATGCCATG GCTGCCATGC CGTCCAGTCA GGAAACAGCA 
GAGGCTGTGC CCAAGGGAGC GATGGGCAAC ATGGGGTCGG TGCCTCCCCA GCCGCCTCCG 
CAGGACGCAG GTGGGGCCCC GGACCACAGC ATGCTCTACT ACTACGGCCA GATCCACATG 
TACGAACAGG ATGGAGGCCT GGAGAACCTC GGGAGCTGCC AGGTCATGCG GTCCCAGCCA 
CCACAGCCAC AGGCCTGTCA GGACAGCATC CAGCCCCAGC CCTTGCCCTC ACCAGGGGTC 
AACCAGGTGT CCAGCACTGT GGACTCCCAG CTCCTGGAGG CCCCCCAGAT TGACTTCGAT 
GCCATCATGG ATGATGGCGA TCACTCGAGT TTGTTCTCGG GTGCTCTGAG CCCCAGCCTC 
CTCCACAGCC TCTCCCAGAA CTCCTCCCGC CTCACCACCC CCCGAAACTC CTTGACCCTG 
CCCTCCATCC CCGCAGGCAT CAGCAACATG GCTGTCGGGG ACATGAGCTC CATGCTCACC 
AGCCTCGCCG AGGAGAGCAA GTTCCTGAAC ATGATGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509274.2
CDS	160..4821
Translation	MTEPWFTLRSVWIHGSVPQHLLPPFHAPLPIDMRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQGQVSGHGSCGCALPLSQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Target ORF information:

RefSeq Version	XM_011510972.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 2 (GLI2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510972.2

ORF Insert Sequence:

ATGACAGAGC CGTGGTTCAC ACTCAGGTCT GTCTGGATTC ATGGCTCAGT GCCGCAGCAT 
CTCTTGCCAC CATTCCATGC GCCCCTACCG ATTGACATGC GACACCAGGA AGGAAGGTAC 
CATTACGAGC CTCATTCTGT CCACGGTGTG CACGGGCCCC CTGCCCTCAG CGGCAGCCCT 
GTCATCTCTG ACATCTCCTT GATCCGGCTT TCCCCGCACC CGGCTGGCCC TGGGGAGTCC 
CCCTTCAACG CCCCCCACCC GTACGTGAAC CCCCACATGG AGCACTACCT CCGTTCTGTG 
CACAGCAGCC CCACGCTCTC CATGATCTCT GCAGCCAGGG GCCTCAGCCC CGCTGATGTG 
GCCCAGGAGC ACCTTAAGGA GAGGGGACTG TTTGGCCTTC CTGCTCCAGG CACCACCCCC 
TCAGACTATT ACCACCAGAT GACCCTCGTG GCAGGCCACC CCGCGCCCTA CGGGGACCTG 
CTGATGCAGA GCGGGGGCGC TGCCAGCGCA CCCCATCTCC ACGACTACCT CAACCCCGTG 
GACGTGTCCC GTTTCTCCAG CCCGCGGGTG ACGCCCCGCC TGAGCCGCAA GCGGGCGCTG 
TCCATCTCCC CACTCTCAGA CGCCAGCCTG GACCTGCAGC GGATGATCCG CACCTCACCC 
AACTCGCTAG TGGCCTACAT CAACAACTCC CGAAGCAGCT CGGCGGCCAG CGGTTCCTAC 
GGGCATCTGT CAGCGGGTGC CCTCAGCCCA GCCTTCACCT TCCCCCACCC CATCAACCCC 
GTGGCCTACC AGCAGATTCT GAGCCAGCAG AGGGGTCTGG GGTCAGCCTT TGGACACACA 
CCACCCCTGA TCCAGCCCTC ACCCACCTTC CTGGCCCAGC AGCCCATGGC CCTCACCTCC 
ATCAATGCCA CGCCCACCCA GCTCAGCAGC AGCAGCAACT GTCTGAGTGA CACCAACCAG 
AACAAGCAGA GCAGTGAGTC GGCCGTCAGC AGCACCGTCA ACCCTGTCGC CATTCACAAG 
CGCAGCAAGG TCAAGACCGA GCCTGAGGGC CTGCGGCCGG CCTCCCCTCT GGCGCTGACG 
CAGGGCCAGG TGTCTGGACA CGGCTCATGT GGGTGTGCCC TTCCCCTCTC CCAGGAGCAG 
CTGGCTGACC TCAAGGAAGA TCTGGACAGG GATGACTGTA AGCAGGAGGC TGAGGTGGTC 
ATCTATGAGA CCAACTGCCA CTGGGAAGAC TGCACCAAGG AGTACGACAC CCAGGAGCAG 
CTGGTGCATC ACATCAACAA CGAGCACATC CACGGGGAGA AGAAGGAGTT TGTGTGCCGC 
TGGCAGGCCT GCACGCGGGA GCAGAAGCCC TTCAAGGCGC AGTACATGCT GGTGGTGCAC 
ATGCGGCGAC ACACGGGCGA GAAGCCCCAC AAGTGCACGT TCGAGGGCTG CTCGAAGGCC 
TACTCCCGCC TGGAGAACCT GAAGACACAC CTGCGGTCCC ACACCGGGGA GAAGCCATAT 
GTGTGTGAGC ACGAGGGCTG CAACAAAGCC TTCTCCAACG CCTCGGACCG CGCCAAGCAC 
CAGAATCGCA CCCACTCCAA CGAGAAACCC TACATCTGCA AGATCCCAGG CTGCACCAAG 
AGATACACAG ACCCCAGCTC TCTCCGGAAG CATGTGAAAA CGGTCCACGG CCCAGATGCC 
CACGTCACCA AGAAGCAGCG CAATGACGTG CACCTCCGCA CACCGCTGCT CAAAGAGAAT 
GGGGACAGTG AGGCCGGCAC GGAGCCTGGC GGCCCAGAGA GCACCGAGGC CAGCAGCACC 
AGCCAGGCCG TGGAGGACTG CCTGCACGTC AGAGCCATCA AGACCGAGAG CTCCGGGCTG 
TGTCAGTCCA GCCCCGGGGC CCAGTCGTCC TGCAGCAGCG AGCCCTCTCC TCTGGGCAGT 
GCCCCCAACA ATGACAGTGG CGTGGAGATG CCGGGGACGG GGCCCGGGAG CCTGGGAGAC 
CTGACGGCAC TGGATGACAC ACCCCCAGGG GCCGACACCT CAGCCCTGGC TGCCCCCTCC 
GCTGGTGGCC TCCAGCTGCG CAAACACATG ACCACCATGC ACCGGTTCGA GCAGCTCAAG 
AAGGAGAAGC TCAAGTCACT CAAGGATTCC TGCTCATGGG CCGGGCCGAC TCCACACACG 
CGGAACACCA AGCTGCCTCC CCTCCCGGGA AGTGGCTCCA TCCTGGAAAA CTTCAGTGGC 
AGTGGGGGCG GCGGGCCCGC GGGGCTGCTG CCGAACCCGC GGCTGTCGGA GCTGTCCGCG 
AGCGAGGTGA CCATGCTGAG CCAGCTGCAG GAGCGCCGCG ACAGCTCCAC CAGCACGGTC 
AGCTCGGCCT ACACCGTGAG CCGCCGCTCC TCCGGCATCT CCCCCTACTT CTCCAGCCGC 
CGCTCCAGCG AGGCCTCGCC CCTGGGCGCC GGCCGCCCGC ACAACGCGAG CTCCGCTGAC 
TCCTACGACC CCATCTCCAC GGACGCGTCG CGGCGCTCGA GCGAGGCCAG CCAGTGCAGC 
GGCGGCTCCG GGCTGCTCAA CCTCACGCCG GCGCAGCAGT ACAGCCTGCG GGCCAAGTAC 
GCGGCAGCCA CTGGCGGCCC CCCGCCCACT CCGCTGCCGG GCCTGGAGCG CATGAGCCTG 
CGGACCAGGC TGGCGCTGCT GGACGCGCCC GAGCGCACGC TGCCCGCCGG CTGCCCACGC 
CCACTGGGGC CGCGGCGTGG CAGCGACGGG CCGACCTATG GCCACGGCCA CGCGGGGGCT 
GCGCCCGCCT TCCCCCACGA GGCTCCAGGC GGCGGAGCCA GGCGGGCCAG CGACCCTGTG 
CGGCGGCCCG ATGCCCTGTC CCTGCCGCGG GTGCAGCGCT TCCACAGCAC CCACAACGTG 
AACCCCGGCC CGCTGCCGCC CTGTGCCGAC AGGCGAGGCC TCCGCCTGCA GAGCCACCCG 
AGCACCGACG GCGGCCTGGC CCGCGGCGCC TACTCGCCCC GGCCGCCTAG CATCAGCGAG 
AACGTGGCGA TGGAGGCCGT GGCGGCAGGA GTGGACGGCG CGGGGCCCGA GGCCGACCTG 
GGGCTGCCGG AGGACGACCT GGTGCTTCCA GACGACGTGG TGCAGTACAT CAAGGCGCAC 
GCCAGTGGCG CTCTGGACGA GGGCACCGGG CAGGTGTATC CCACGGAAAG CACTGGCTTC 
TCTGACAACC CCAGACTACC CAGCCCGGGG CTGCACGGCC AGCGCAGGAT GGTGGCTGCG 
GACTCCAACG TGGGCCCCTC CGCCCCTATG CTGGGAGGAT GCCAGTTAGG CTTTGGGGCG 
CCCTCCAGCC TGAACAAAAA TAACATGCCT GTGCAGTGGA ATGAGGTGAG CTCCGGCACC 
GTAGACGCCC TGGCCAGCCA GGTGAAGCCT CCACCCTTTC CTCAGGGCAA CCTGGCGGTG 
GTGCAGCAGA AGCCTGCCTT TGGCCAGTAC CCGGGCTACA GTCCGCAAGG CCTACAGGCT 
AGCCCTGGGG GCCTGGACAG CACGCAGCCA CACCTGCAGC CCCGCAGCGG AGCCCCCTCC 
CAGGGCATCC CCAGGGTAAA CTACATGCAG CAGCTGCGAC AGCCAGTGGC AGGCAGCCAG 
TGTCCTGGCA TGACTACCAC TATGAGCCCC CATGCCTGCT ATGGCCAAGT CCACCCCCAG 
CTGAGCCCCA GCACCATCAG TGGGGCCCTC AACCAGTTCC CCCAATCCTG CAGCAACATG 
CCAGCCAAGC CAGGGCATCT GGGGCACCCT CAGCAGACAG AAGTGGCACC TGACCCCACC 
ACGATGGGCA ATCGCCACAG GGAACTTGGG GTCCCCGATT CAGCCCTGGC TGGAGTGCCA 
CCACCTCACC CAGTCCAGAG CTACCCACAG CAGAGCCATC ACCTGGCAGC CTCCATGAGC 
CAGGAGGGCT ACCACCAGGT CCCCAGCCTT CTGCCTGCCC GCCAGCCTGG CTTCATGGAG 
CCCCAAACAG GCCCGATGGG GGTGGCTACA GCAGGCTTTG GCCTAGTGCA GCCCCGGCCT 
CCCCTCGAGC CCAGCCCCAC TGGCCGCCAC CGTGGGGTAC GTGCTGTGCA GCAGCAGCTG 
GCCTACGCCA GGGCCACAGG CCATGCCATG GCTGCCATGC CGTCCAGTCA GGAAACAGCA 
GAGGCTGTGC CCAAGGGAGC GATGGGCAAC ATGGGGTCGG TGCCTCCCCA GCCGCCTCCG 
CAGGACGCAG GTGGGGCCCC GGACCACAGC ATGCTCTACT ACTACGGCCA GATCCACATG 
TACGAACAGG ATGGAGGCCT GGAGAACCTC GGGAGCTGCC AGGTCATGCG GTCCCAGCCA 
CCACAGCCAC AGGCCTGTCA GGACAGCATC CAGCCCCAGC CCTTGCCCTC ACCAGGGGTC 
AACCAGGTGT CCAGCACTGT GGACTCCCAG CTCCTGGAGG CCCCCCAGAT TGACTTCGAT 
GCCATCATGG ATGATGGCGA TCACTCGAGT TTGTTCTCGG GTGCTCTGAG CCCCAGCCTC 
CTCCACAGCC TCTCCCAGAA CTCCTCCCGC CTCACCACCC CCCGAAACTC CTTGACCCTG 
CCCTCCATCC CCGCAGGCAT CAGCAACATG GCTGTCGGGG ACATGAGCTC CATGCTCACC 
AGCCTCGCCG AGGAGAGCAA GTTCCTGAAC ATGATGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GLI2
Entrez Gene ID	2736
Full Name	GLI family zinc finger 2
Synonyms	CJS,HPE9,PHS2,THP1,THP2
General protein information	Preferred Names GLI family zinc finger 2 Names zinc finger protein GLI2 GLI family zinc finger protein 2 GLI-Kruppel family member GLI2 glioma-associated oncogene family zinc finger 2 oncogene GLI2 tax helper protein 1 tax helper protein 2 tax-responsive element-2 holding protein tax-responsive element-25-bp sequence binding protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q14.2
Summary	This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008].
Disorder MIM:	165230
Disorder Html:	Holoprosencephaly 9, 610829 (3); Culler-Jones syndrome, 615849 (3)

Mus musculus (house mouse)	Gli2	NP_001074594.1
Xenopus tropicalis (tropical clawed frog)	LOC100498653	XP_002943329.2
Homo sapiens (human)	GLI2	NP_005261.2
Canis lupus familiaris (dog)	GLI2	XP_003432520.1
Rattus norvegicus (Norway rat)	Gli2	NP_001100639.1
Danio rerio (zebrafish)	gli2a	NP_571042.1
Bos taurus (cattle)	GLI2	NP_001179179.2
Pan troglodytes (chimpanzee)	GLI2	XP_001158280.2
	LOC705877	XP_002808099.1
Gallus gallus (chicken)	GLI2	NP_001258830.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

GLI2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

ORF Insert Sequence:

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