gRNAs
The following Gli2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gli2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa11983 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017598785.1 , NM_001107169.1 | |
| Accession Version | NM_001107169.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-30 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | zinc finger protein GLI2 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474028.1. On or before Oct 4, 2007 this sequence version replaced XM_001053566.1, XM_222557.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGAGACACC AGGAGGGAAG GTACCATTAT GACCCTCACC CTGTCCACGG TGTGCACGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001100639.1 |
| CDS | 49..4491 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001107169.1 |
1 | ATGAGACACC AGGAGGGAAG GTACCATTAT GACCCTCACC CTGTCCACGG TGTGCACGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa11983 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017598785.1 , NM_001107169.1 | |
| Accession Version | XM_017598785.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | zinc finger protein GLI2 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGACACC AGGAGGGAAG GTACCATTAT GACCCTCACC CTGTCCACGG TGTGCACGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017454274.1 |
| CDS | 1846..6288 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017598785.1 |
1 | ATGAGACACC AGGAGGGAAG GTACCATTAT GACCCTCACC CTGTCCACGG TGTGCACGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa35222 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008769455.2 , XM_017598784.1 | |
| Accession Version | XM_008769455.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | zinc finger protein GLI2 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769455.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGACAT CAGCCCAAGC TCCTGCACTG GAGAAGAAAG AAGCCAAGAG TGGTCTCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008767677.1 |
| CDS | 808..5442 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_008769455.2 |
1 | ATGGAGACAT CAGCCCAAGC TCCTGCACTG GAGAAGAAAG AAGCCAAGAG TGGTCTCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa35222 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008769455.2 , XM_017598784.1 | |
| Accession Version | XM_017598784.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | zinc finger protein GLI2 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGACAT CAGCCCAAGC TCCTGCACTG GAGAAGAAAG AAGCCAAGAG TGGTCTCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017454273.1 |
| CDS | 923..5557 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017598784.1 |
1 | ATGGAGACAT CAGCCCAAGC TCCTGCACTG GAGAAGAAAG AAGCCAAGAG TGGTCTCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Sonic Hedgehog Signaling Affected by Promoter Hypermethylation Induces Aberrant Gli2 Expression in Spina Bifida. |
FOXC1 Activates Smoothened-Independent Hedgehog Signaling in Basal-like Breast Cancer. |
The transcription factor Foxc1 is necessary for Ihh-Gli2-regulated endochondral ossification. |
Sonic hedgehog (SHH) and glioblastoma-2 (Gli-2) expressions are associated with poor jaundice-free survival in biliary atresia. |
Bifurcating action of Smoothened in Hedgehog signaling is mediated by Dlg5. |
