GLI2 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	GLI2
Entrez Gene ID	395956
Full Name	GLI family zinc finger 2
Synonyms	GLI2/GLI4,GLI3
General protein information	Preferred Names GLI family zinc finger 2 Names zinc finger protein GLI2 GLI-Kruppel family member GLI2 Zn finger transcription factor zinc finger protein GLI3
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015289787.2	XP_015145273.1	zinc finger protein GLI2 isoform X2
XM_015289787.1	XP_015145273.1	zinc finger protein GLI2 isoform X2
NM_001271902.1	NP_001258831.1	zinc finger protein GLI2 isoform 2
NM_001271901.1	NP_001258830.1	zinc finger protein GLI2 isoform 1
XM_025152142.1	XP_025007910.1	zinc finger protein GLI2 isoform X1
XM_015289786.1	XP_015145272.1	zinc finger protein GLI2 isoform X1
XM_015289786.2	XP_015145272.1	zinc finger protein GLI2 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gli2	NP_001074594.1
Xenopus tropicalis (tropical clawed frog)	LOC100498653	XP_002943329.2
Homo sapiens (human)	GLI2	NP_005261.2
Canis lupus familiaris (dog)	GLI2	XP_003432520.1
Rattus norvegicus (Norway rat)	Gli2	NP_001100639.1
Danio rerio (zebrafish)	gli2a	NP_571042.1
Bos taurus (cattle)	GLI2	NP_001179179.2
Pan troglodytes (chimpanzee)	GLI2	XP_001158280.2
	LOC705877	XP_002808099.1
Gallus gallus (chicken)	GLI2	NP_001258830.1

Gene Ontology
Bibliography

Related articles in PubMed

A potential role of alternative splicing in the regulation of the transcriptional activity of human GLI2 in gonadal tissues.
Speek M, Njunkova O, Pata I, Valdre E, Kogerman P
BMC molecular biology713(2006 Mar)

Similar expression and regulation of Gli2 and Gli3 in the chick limb bud.
Schweitzer R, Vogan KJ, Tabin CJ
Mechanisms of development98(1-2)171-4(2000 Nov)

Sonic hedgehog differentially regulates expression of GLI and GLI3 during limb development.
Marigo V, Johnson RL, Vortkamp A, Tabin CJ
Developmental biology180(1)273-83(1996 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

comparison of human, mouse, zebrafish and chicken GLI2 proteins
Title: A potential role of alternative splicing in the regulation of the transcriptional activity of human GLI2 in gonadal tissues.

The following GLI2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLI2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa38541	XM_015289787.2 Latest version!	Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa00089	NM_001271902.1 Latest version!	Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OGa00144	NM_001271901.1 Latest version!	Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa00144	XM_025152142.1 Latest version!	Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa00144	XM_015289786.2 Latest version!	Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OGa38541	XM_015289787.1	Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa00144	XM_015289786.1	Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GLI2 ( XM_015289787.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145273.1 Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa38541
Clone ID Related Accession (Same CDS sequence)	XM_015289787.2 , XM_015289787.1
Accession Version	XM_015289787.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	zinc finger protein GLI2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289787.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA 
GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC 
ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG 
CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT 
CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC 
ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC 
AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG 
CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG 
GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG 
GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG 
ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT 
GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC 
AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT 
GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG 
AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT 
CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC 
AACAGCAACT GCATCTCCGA CTCCAGTCAG GAGCATCTGA CAGACCTGAA AGAAGATCTA 
GATAAGGATG AATGTAAACA GGAGCCTGAG GTTATTTATG AGACTAACTG TCACTGGGAA 
GGGTGCACAA AGGAATATGA CACTCAAGAG CAGCTCGTCC ATCACATCAA CAATGATCAT 
ATCCATGGGG AGAAGAAGGA GTTTGTCTGC CGCTGGCAGG ACTGCACGCG GGAGCAGAAG 
CCCTTCAAAG CTCAATACAT GCTGGTGGTG CACATGCGAA GGCACACTGG AGAGAAGCCC 
CACAAGTGCA CGTTTGAGGG TTGCTCCAAG GCTTACTCCC GCCTGGAGAA CTTAAAGACA 
CACCTGAGGT CCCACACTGG AGAAAAACCT TACGTCTGTG AACACGAAGG CTGCAATAAA 
GCCTTTTCCA ATGCCTCGGA TAGAGCCAAG CACCAGAACA GGACGCATTC CAATGAGAAA 
CCCTATGTCT GTAAGATCCC TGGCTGCACA AAGAGGTACA CAGACCCTAG TTCCCTCAGG 
AAACACGTCA AGACCGTACA CGGACCTGAT GCCCACGTCA CAAAGAAACA GCGCAACGAT 
GTCCACCCAA GGCCGCCTCC GCTCAAGGAA AATGGGGACA ACGAGGCGAG CGCCAAGCAG 
AGCAGTAAGG TGTCAGAGGA GAGCCCCGAG GCCAACAGTA CCACGAGGAG CATGGAGGAT 
TGCTTACAAG TCAAAACGAT AAAAACAGAG AATTCTGTGA TGTGTCAGTC CAGTCCTGGT 
GGCCAGTCAT CATGCAGCAG TGAGCCGTCA CCCCTGGGCA GCACCAACAA CAATGACAGT 
GGAGTAGAAA TGAACATGCA CAGCGGGGGA AGTCTGGGAG ATCTGACGGC GTTGGATGAC 
AACGCTCCTG TTGTGGACTC AACGGTCTCA TCTGGTAACT TGACAGTCAG CCTGCAGCTG 
AGGAAACACA TGACGACAAT GCAACGACTT GAACAGCTCA AGAAAGAGAA ACTCAAGACA 
GTTAAGGATT CCTGCTCATG GGTGAACCCA GCTCCACAAG GCAGAAACAC CAAGCTGCCT 
CCCATTTCAG GAAATGGCTC TATTCTAGAA AACAGTGGTG GTTCTTCTGC TACGCTGCCT 
AACCCAAGAA TAATGGAGCT GTCTGTCAAT GAGGTTACGA TGCTGAACCA AATCAATGAG 
CGCCGTGATA GTACGACAAG CACCATCAGC TCTGCCTACA CCGTGAGCCG CAGATCATCA 
GGGATTTCCC CATACTTCTC CAGCCGCCGC TCCAGCGAGG CTTCACAGCT CGGGCACCGT 
CCCAATAACA CGAGCTCTGC TGACTCTTAT GATCCGATTT CAACGGATGC TTCCCGCCGG 
TCAAGTGAGG CGAGCCAGTG CAGCGGGATG CCGGGCCTAC TGAACCTCAC ACCAGCTCAG 
CACTACAGGC TGAAAGCCAA GTATGCTGCT GCCACAGGGG GCCCTCCTCC AACGCCCCTT 
CCAAACATGG AGAGGATGAC CCTGAAGAAC AGAATCTCAC TTATGGATGG ACCAGAGCCC 
ACCTTGCCCT CCATTCGCCT CCCACCAGGC CCCAGGCGTT GCAGTGATGG CAACACCTAT 
GGCTACACAT CCACCGCAGC ATTTCCCCAT GAGGTTCCGG GCAACTGCAC GAGACGGGCG 
AGCGACCCTG TGAGGAGACC TGCTGGAGAG CCCCAGGCCC TCCCGAGAGT TCACCGCTTC 
AACAGCACCA ACAGCATGAA CCCCTTCCAT CCTCCACATC CTGCAGACCG GAGGAATTTC 
AGCATGCAGA GCTATGGGCG CTCTGACGGG AGTCTTGCCC GGCACACCTA CTCACCCCGG 
CCACTGAGCA TCAGTGAAAA CGTCACTATG GAAGCCATGT CTGGGGAGGC AGAGGTGCCT 
GTTGGAGATG ATGACATTAT GCTGCCAGAT GATGTGGTGC AGTACATCAA ATCTCAGAAC 
AACGGGACAG CAGCTGAGAG CACTTCCATC GGGTACAGCA ATGAGATGCA GAGCTTCCAG 
GGAGGCGGGA AGCTGCAGCC CCCAGCCTTT CCCAGCCAGC GCAGGATGGC GGTGGCCGAC 
GCGAGCATGA GCCATTTGGG GCCCATGATG GCAGAGTGTC CCATGAGCTT TGACGCGCCC 
TCAGACATGA ATAAAAATAA CATGCCTGTC CAGTGGAACG AAGTAAGCTC GGGTACAGTT 
GATCTCATGT CCAATCAATC CAAGCAGCAG TTTTCGCAAG GGAACTTAGC AGTGGTCCAG 
CAGAAGCAGA ACTTTGGTCA GTACCAGAGC TACAACCAGC AGCAGATGCA GCTACCAGAG 
AACAGCATGA ATGTAACACA GCAGAGCTTT ATGCAAAGAA ATGTGGGCAT GGATGGGCAG 
AGGCTAAACT GTATGCAGCT GCGGCAGCAG CCAATGAGCC TAGGCCCAGG GATGAACCCT 
GATCTGGGCT CGCAGGCAGG GTATAACCAA GCTCATCAGA TGCTGAGCCC CAGTGCAATC 
AATGGCAACC CAAATCAGCT CTCTCCTTCT TGTAGCAACA TGGTGACAAA GTCTGGGCCC 
CACCTCCACC CTCAGCAAAT GGACATGGCG ACCGACCCAT CCTTAATGGT CAGCAGCAAC 
AGCGAGCGCA CAATGCTGAA CCAGGTCATG CACGAACCGA GCCAGCAGAA CTACTCATCT 
CAGCCAACCC ACATGAACTT CCCCATGGCT CAGGAGGCTT TCCATCAGCC CATCATGCCC 
TCCAACCAGC AGAGCTTTGA GCCCCAGCAG AGCATGATGG GTTCGGCGGC GCAGACGTAT 
CCACCCGGCA TGGTCCAACC TCGTCCCCCC GCTGAGCCCA GCCCGGCCAA CAGGCCTCGA 
GGCCTCCGCA CCGTGCAGCA GCTGGGCTAC ATGAGAACTC CCCACCCTAC AAACACCATG 
AGCCCGGGCC AGGAGGCGGT GGAGGCTGTG CATAAAAGAA CCGGCGACGT GTTGCCAGCA 
TCGACCCAAC AGTGCGCGGA TGGCACGAGG GAAAATAATT TGATGTACTA TTATGGTCAA 
ATCCACATGT ACGAACAAAA CAGCGGCTTT GATAATCACT CGGACTGCAG GGTCAGGCAG 
CAGCAGTGCG CACTGAGCGG CAAGCCGGCC GCTCTGCCAT CCCCGGGCAC CAACCAGGTG 
TCCAGCACGG TGGACTCACA AGGTCTTGAG CCGCCCCAGA TAGATTTTGA TGCCATCATG 
GACGATGGGG ACCACTCGAG CCTGATGTCA GGAACCCTGA GCCCCAGCAT CCTGCAGAAC 
CTCTCCCAGA ACTCCTCTCG CCTGACGACT CCACGAAACT CTCTGACTCT GCCCACCATA 
CCCACGGGAA TAAGCAATAT GGCAATAGGA GATATGAGCT CCATGCTGAC CACCCTGGCA 
GAAGAGAGTA AATTTCTCAA CATGATGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145273.1
CDS	315..4787
Translation	METSASTVAGKKEGKGVVLEGDGFTETGKKPTPLAAAGAAVAQGVPQHIFPAFHAPLPIDMRHQEGRYHYEPHSIHAIHGPPPLSGSPVISDISLIRLSPHPAGPGESPFSPPHPYVTPHMEHYLRSVHGSPTLSVISAARGLSPADVAHEHLKERGLFGLPPPPPGANPTDYYHQMTLMAGHPNPYGDILMQSGGAAGTAHLHEYLSPVDVSRFSSPRVTPRLSRKRALSISPLSDASIDLQTMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGTISPAFSFPHPINPVTYQQILTQQRGLSSAFGHTPPLIQPSPTFPPRQHMAVISVNPPPAQISSNSNCISDSSQEHLTDLKEDLDKDECKQEPEVIYETNCHWEGCTKEYDTQEQLVHHINNDHIHGEKKEFVCRWQDCTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHPRPPPLKENGDNEASAKQSSKVSEESPEANSTTRSMEDCLQVKTIKTENSVMCQSSPGGQSSCSSEPSPLGSTNNNDSGVEMNMHSGGSLGDLTALDDNAPVVDSTVSSGNLTVSLQLRKHMTTMQRLEQLKKEKLKTVKDSCSWVNPAPQGRNTKLPPISGNGSILENSGGSSATLPNPRIMELSVNEVTMLNQINERRDSTTSTISSAYTVSRRSSGISPYFSSRRSSEASQLGHRPNNTSSADSYDPISTDASRRSSEASQCSGMPGLLNLTPAQHYRLKAKYAAATGGPPPTPLPNMERMTLKNRISLMDGPEPTLPSIRLPPGPRRCSDGNTYGYTSTAAFPHEVPGNCTRRASDPVRRPAGEPQALPRVHRFNSTNSMNPFHPPHPADRRNFSMQSYGRSDGSLARHTYSPRPLSISENVTMEAMSGEAEVPVGDDDIMLPDDVVQYIKSQNNGTAAESTSIGYSNEMQSFQGGGKLQPPAFPSQRRMAVADASMSHLGPMMAECPMSFDAPSDMNKNNMPVQWNEVSSGTVDLMSNQSKQQFSQGNLAVVQQKQNFGQYQSYNQQQMQLPENSMNVTQQSFMQRNVGMDGQRLNCMQLRQQPMSLGPGMNPDLGSQAGYNQAHQMLSPSAINGNPNQLSPSCSNMVTKSGPHLHPQQMDMATDPSLMVSSNSERTMLNQVMHEPSQQNYSSQPTHMNFPMAQEAFHQPIMPSNQQSFEPQQSMMGSAAQTYPPGMVQPRPPAEPSPANRPRGLRTVQQLGYMRTPHPTNTMSPGQEAVEAVHKRTGDVLPASTQQCADGTRENNLMYYYGQIHMYEQNSGFDNHSDCRVRQQQCALSGKPAALPSPGTNQVSSTVDSQGLEPPQIDFDAIMDDGDHSSLMSGTLSPSILQNLSQNSSRLTTPRNSLTLPTIPTGISNMAIGDMSSMLTTLAEESKFLNMMS

Target ORF information:

RefSeq Version	XM_015289787.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289787.2

ORF Insert Sequence:

ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA 
GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC 
ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG 
CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT 
CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC 
ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC 
AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG 
CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG 
GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG 
GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG 
ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT 
GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC 
AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT 
GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG 
AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT 
CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC 
AACAGCAACT GCATCTCCGA CTCCAGTCAG GAGCATCTGA CAGACCTGAA AGAAGATCTA 
GATAAGGATG AATGTAAACA GGAGCCTGAG GTTATTTATG AGACTAACTG TCACTGGGAA 
GGGTGCACAA AGGAATATGA CACTCAAGAG CAGCTCGTCC ATCACATCAA CAATGATCAT 
ATCCATGGGG AGAAGAAGGA GTTTGTCTGC CGCTGGCAGG ACTGCACGCG GGAGCAGAAG 
CCCTTCAAAG CTCAATACAT GCTGGTGGTG CACATGCGAA GGCACACTGG AGAGAAGCCC 
CACAAGTGCA CGTTTGAGGG TTGCTCCAAG GCTTACTCCC GCCTGGAGAA CTTAAAGACA 
CACCTGAGGT CCCACACTGG AGAAAAACCT TACGTCTGTG AACACGAAGG CTGCAATAAA 
GCCTTTTCCA ATGCCTCGGA TAGAGCCAAG CACCAGAACA GGACGCATTC CAATGAGAAA 
CCCTATGTCT GTAAGATCCC TGGCTGCACA AAGAGGTACA CAGACCCTAG TTCCCTCAGG 
AAACACGTCA AGACCGTACA CGGACCTGAT GCCCACGTCA CAAAGAAACA GCGCAACGAT 
GTCCACCCAA GGCCGCCTCC GCTCAAGGAA AATGGGGACA ACGAGGCGAG CGCCAAGCAG 
AGCAGTAAGG TGTCAGAGGA GAGCCCCGAG GCCAACAGTA CCACGAGGAG CATGGAGGAT 
TGCTTACAAG TCAAAACGAT AAAAACAGAG AATTCTGTGA TGTGTCAGTC CAGTCCTGGT 
GGCCAGTCAT CATGCAGCAG TGAGCCGTCA CCCCTGGGCA GCACCAACAA CAATGACAGT 
GGAGTAGAAA TGAACATGCA CAGCGGGGGA AGTCTGGGAG ATCTGACGGC GTTGGATGAC 
AACGCTCCTG TTGTGGACTC AACGGTCTCA TCTGGTAACT TGACAGTCAG CCTGCAGCTG 
AGGAAACACA TGACGACAAT GCAACGACTT GAACAGCTCA AGAAAGAGAA ACTCAAGACA 
GTTAAGGATT CCTGCTCATG GGTGAACCCA GCTCCACAAG GCAGAAACAC CAAGCTGCCT 
CCCATTTCAG GAAATGGCTC TATTCTAGAA AACAGTGGTG GTTCTTCTGC TACGCTGCCT 
AACCCAAGAA TAATGGAGCT GTCTGTCAAT GAGGTTACGA TGCTGAACCA AATCAATGAG 
CGCCGTGATA GTACGACAAG CACCATCAGC TCTGCCTACA CCGTGAGCCG CAGATCATCA 
GGGATTTCCC CATACTTCTC CAGCCGCCGC TCCAGCGAGG CTTCACAGCT CGGGCACCGT 
CCCAATAACA CGAGCTCTGC TGACTCTTAT GATCCGATTT CAACGGATGC TTCCCGCCGG 
TCAAGTGAGG CGAGCCAGTG CAGCGGGATG CCGGGCCTAC TGAACCTCAC ACCAGCTCAG 
CACTACAGGC TGAAAGCCAA GTATGCTGCT GCCACAGGGG GCCCTCCTCC AACGCCCCTT 
CCAAACATGG AGAGGATGAC CCTGAAGAAC AGAATCTCAC TTATGGATGG ACCAGAGCCC 
ACCTTGCCCT CCATTCGCCT CCCACCAGGC CCCAGGCGTT GCAGTGATGG CAACACCTAT 
GGCTACACAT CCACCGCAGC ATTTCCCCAT GAGGTTCCGG GCAACTGCAC GAGACGGGCG 
AGCGACCCTG TGAGGAGACC TGCTGGAGAG CCCCAGGCCC TCCCGAGAGT TCACCGCTTC 
AACAGCACCA ACAGCATGAA CCCCTTCCAT CCTCCACATC CTGCAGACCG GAGGAATTTC 
AGCATGCAGA GCTATGGGCG CTCTGACGGG AGTCTTGCCC GGCACACCTA CTCACCCCGG 
CCACTGAGCA TCAGTGAAAA CGTCACTATG GAAGCCATGT CTGGGGAGGC AGAGGTGCCT 
GTTGGAGATG ATGACATTAT GCTGCCAGAT GATGTGGTGC AGTACATCAA ATCTCAGAAC 
AACGGGACAG CAGCTGAGAG CACTTCCATC GGGTACAGCA ATGAGATGCA GAGCTTCCAG 
GGAGGCGGGA AGCTGCAGCC CCCAGCCTTT CCCAGCCAGC GCAGGATGGC GGTGGCCGAC 
GCGAGCATGA GCCATTTGGG GCCCATGATG GCAGAGTGTC CCATGAGCTT TGACGCGCCC 
TCAGACATGA ATAAAAATAA CATGCCTGTC CAGTGGAACG AAGTAAGCTC GGGTACAGTT 
GATCTCATGT CCAATCAATC CAAGCAGCAG TTTTCGCAAG GGAACTTAGC AGTGGTCCAG 
CAGAAGCAGA ACTTTGGTCA GTACCAGAGC TACAACCAGC AGCAGATGCA GCTACCAGAG 
AACAGCATGA ATGTAACACA GCAGAGCTTT ATGCAAAGAA ATGTGGGCAT GGATGGGCAG 
AGGCTAAACT GTATGCAGCT GCGGCAGCAG CCAATGAGCC TAGGCCCAGG GATGAACCCT 
GATCTGGGCT CGCAGGCAGG GTATAACCAA GCTCATCAGA TGCTGAGCCC CAGTGCAATC 
AATGGCAACC CAAATCAGCT CTCTCCTTCT TGTAGCAACA TGGTGACAAA GTCTGGGCCC 
CACCTCCACC CTCAGCAAAT GGACATGGCG ACCGACCCAT CCTTAATGGT CAGCAGCAAC 
AGCGAGCGCA CAATGCTGAA CCAGGTCATG CACGAACCGA GCCAGCAGAA CTACTCATCT 
CAGCCAACCC ACATGAACTT CCCCATGGCT CAGGAGGCTT TCCATCAGCC CATCATGCCC 
TCCAACCAGC AGAGCTTTGA GCCCCAGCAG AGCATGATGG GTTCGGCGGC GCAGACGTAT 
CCACCCGGCA TGGTCCAACC TCGTCCCCCC GCTGAGCCCA GCCCGGCCAA CAGGCCTCGA 
GGCCTCCGCA CCGTGCAGCA GCTGGGCTAC ATGAGAACTC CCCACCCTAC AAACACCATG 
AGCCCGGGCC AGGAGGCGGT GGAGGCTGTG CATAAAAGAA CCGGCGACGT GTTGCCAGCA 
TCGACCCAAC AGTGCGCGGA TGGCACGAGG GAAAATAATT TGATGTACTA TTATGGTCAA 
ATCCACATGT ACGAACAAAA CAGCGGCTTT GATAATCACT CGGACTGCAG GGTCAGGCAG 
CAGCAGTGCG CACTGAGCGG CAAGCCGGCC GCTCTGCCAT CCCCGGGCAC CAACCAGGTG 
TCCAGCACGG TGGACTCACA AGGTCTTGAG CCGCCCCAGA TAGATTTTGA TGCCATCATG 
GACGATGGGG ACCACTCGAG CCTGATGTCA GGAACCCTGA GCCCCAGCAT CCTGCAGAAC 
CTCTCCCAGA ACTCCTCTCG CCTGACGACT CCACGAAACT CTCTGACTCT GCCCACCATA 
CCCACGGGAA TAAGCAATAT GGCAATAGGA GATATGAGCT CCATGCTGAC CACCCTGGCA 
GAAGAGAGTA AATTTCTCAA CATGATGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( XM_015289787.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145273.1 Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa38541
Clone ID Related Accession (Same CDS sequence)	XM_015289787.2 , XM_015289787.1
Accession Version	XM_015289787.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	zinc finger protein GLI2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA 
GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC 
ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG 
CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT 
CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC 
ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC 
AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG 
CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG 
GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG 
GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG 
ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT 
GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC 
AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT 
GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG 
AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT 
CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC 
AACAGCAACT GCATCTCCGA CTCCAGTCAG GAGCATCTGA CAGACCTGAA AGAAGATCTA 
GATAAGGATG AATGTAAACA GGAGCCTGAG GTTATTTATG AGACTAACTG TCACTGGGAA 
GGGTGCACAA AGGAATATGA CACTCAAGAG CAGCTCGTCC ATCACATCAA CAATGATCAT 
ATCCATGGGG AGAAGAAGGA GTTTGTCTGC CGCTGGCAGG ACTGCACGCG GGAGCAGAAG 
CCCTTCAAAG CTCAATACAT GCTGGTGGTG CACATGCGAA GGCACACTGG AGAGAAGCCC 
CACAAGTGCA CGTTTGAGGG TTGCTCCAAG GCTTACTCCC GCCTGGAGAA CTTAAAGACA 
CACCTGAGGT CCCACACTGG AGAAAAACCT TACGTCTGTG AACACGAAGG CTGCAATAAA 
GCCTTTTCCA ATGCCTCGGA TAGAGCCAAG CACCAGAACA GGACGCATTC CAATGAGAAA 
CCCTATGTCT GTAAGATCCC TGGCTGCACA AAGAGGTACA CAGACCCTAG TTCCCTCAGG 
AAACACGTCA AGACCGTACA CGGACCTGAT GCCCACGTCA CAAAGAAACA GCGCAACGAT 
GTCCACCCAA GGCCGCCTCC GCTCAAGGAA AATGGGGACA ACGAGGCGAG CGCCAAGCAG 
AGCAGTAAGG TGTCAGAGGA GAGCCCCGAG GCCAACAGTA CCACGAGGAG CATGGAGGAT 
TGCTTACAAG TCAAAACGAT AAAAACAGAG AATTCTGTGA TGTGTCAGTC CAGTCCTGGT 
GGCCAGTCAT CATGCAGCAG TGAGCCGTCA CCCCTGGGCA GCACCAACAA CAATGACAGT 
GGAGTAGAAA TGAACATGCA CAGCGGGGGA AGTCTGGGAG ATCTGACGGC GTTGGATGAC 
AACGCTCCTG TTGTGGACTC AACGGTCTCA TCTGGTAACT TGACAGTCAG CCTGCAGCTG 
AGGAAACACA TGACGACAAT GCAACGACTT GAACAGCTCA AGAAAGAGAA ACTCAAGACA 
GTTAAGGATT CCTGCTCATG GGTGAACCCA GCTCCACAAG GCAGAAACAC CAAGCTGCCT 
CCCATTTCAG GAAATGGCTC TATTCTAGAA AACAGTGGTG GTTCTTCTGC TACGCTGCCT 
AACCCAAGAA TAATGGAGCT GTCTGTCAAT GAGGTTACGA TGCTGAACCA AATCAATGAG 
CGCCGTGATA GTACGACAAG CACCATCAGC TCTGCCTACA CCGTGAGCCG CAGATCATCA 
GGGATTTCCC CATACTTCTC CAGCCGCCGC TCCAGCGAGG CTTCACAGCT CGGGCACCGT 
CCCAATAACA CGAGCTCTGC TGACTCTTAT GATCCGATTT CAACGGATGC TTCCCGCCGG 
TCAAGTGAGG CGAGCCAGTG CAGCGGGATG CCGGGCCTAC TGAACCTCAC ACCAGCTCAG 
CACTACAGGC TGAAAGCCAA GTATGCTGCT GCCACAGGGG GCCCTCCTCC AACGCCCCTT 
CCAAACATGG AGAGGATGAC CCTGAAGAAC AGAATCTCAC TTATGGATGG ACCAGAGCCC 
ACCTTGCCCT CCATTCGCCT CCCACCAGGC CCCAGGCGTT GCAGTGATGG CAACACCTAT 
GGCTACACAT CCACCGCAGC ATTTCCCCAT GAGGTTCCGG GCAACTGCAC GAGACGGGCG 
AGCGACCCTG TGAGGAGACC TGCTGGAGAG CCCCAGGCCC TCCCGAGAGT TCACCGCTTC 
AACAGCACCA ACAGCATGAA CCCCTTCCAT CCTCCACATC CTGCAGACCG GAGGAATTTC 
AGCATGCAGA GCTATGGGCG CTCTGACGGG AGTCTTGCCC GGCACACCTA CTCACCCCGG 
CCACTGAGCA TCAGTGAAAA CGTCACTATG GAAGCCATGT CTGGGGAGGC AGAGGTGCCT 
GTTGGAGATG ATGACATTAT GCTGCCAGAT GATGTGGTGC AGTACATCAA ATCTCAGAAC 
AACGGGACAG CAGCTGAGAG CACTTCCATC GGGTACAGCA ATGAGATGCA GAGCTTCCAG 
GGAGGCGGGA AGCTGCAGCC CCCAGCCTTT CCCAGCCAGC GCAGGATGGC GGTGGCCGAC 
GCGAGCATGA GCCATTTGGG GCCCATGATG GCAGAGTGTC CCATGAGCTT TGACGCGCCC 
TCAGACATGA ATAAAAATAA CATGCCTGTC CAGTGGAACG AAGTAAGCTC GGGTACAGTT 
GATCTCATGT CCAATCAATC CAAGCAGCAG TTTTCGCAAG GGAACTTAGC AGTGGTCCAG 
CAGAAGCAGA ACTTTGGTCA GTACCAGAGC TACAACCAGC AGCAGATGCA GCTACCAGAG 
AACAGCATGA ATGTAACACA GCAGAGCTTT ATGCAAAGAA ATGTGGGCAT GGATGGGCAG 
AGGCTAAACT GTATGCAGCT GCGGCAGCAG CCAATGAGCC TAGGCCCAGG GATGAACCCT 
GATCTGGGCT CGCAGGCAGG GTATAACCAA GCTCATCAGA TGCTGAGCCC CAGTGCAATC 
AATGGCAACC CAAATCAGCT CTCTCCTTCT TGTAGCAACA TGGTGACAAA GTCTGGGCCC 
CACCTCCACC CTCAGCAAAT GGACATGGCG ACCGACCCAT CCTTAATGGT CAGCAGCAAC 
AGCGAGCGCA CAATGCTGAA CCAGGTCATG CACGAACCGA GCCAGCAGAA CTACTCATCT 
CAGCCAACCC ACATGAACTT CCCCATGGCT CAGGAGGCTT TCCATCAGCC CATCATGCCC 
TCCAACCAGC AGAGCTTTGA GCCCCAGCAG AGCATGATGG GTTCGGCGGC GCAGACGTAT 
CCACCCGGCA TGGTCCAACC TCGTCCCCCC GCTGAGCCCA GCCCGGCCAA CAGGCCTCGA 
GGCCTCCGCA CCGTGCAGCA GCTGGGCTAC ATGAGAACTC CCCACCCTAC AAACACCATG 
AGCCCGGGCC AGGAGGCGGT GGAGGCTGTG CATAAAAGAA CCGGCGACGT GTTGCCAGCA 
TCGACCCAAC AGTGCGCGGA TGGCACGAGG GAAAATAATT TGATGTACTA TTATGGTCAA 
ATCCACATGT ACGAACAAAA CAGCGGCTTT GATAATCACT CGGACTGCAG GGTCAGGCAG 
CAGCAGTGCG CACTGAGCGG CAAGCCGGCC GCTCTGCCAT CCCCGGGCAC CAACCAGGTG 
TCCAGCACGG TGGACTCACA AGGTCTTGAG CCGCCCCAGA TAGATTTTGA TGCCATCATG 
GACGATGGGG ACCACTCGAG CCTGATGTCA GGAACCCTGA GCCCCAGCAT CCTGCAGAAC 
CTCTCCCAGA ACTCCTCTCG CCTGACGACT CCACGAAACT CTCTGACTCT GCCCACCATA 
CCCACGGGAA TAAGCAATAT GGCAATAGGA GATATGAGCT CCATGCTGAC CACCCTGGCA 
GAAGAGAGTA AATTTCTCAA CATGATGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145273.1
CDS	316..4788
Translation	METSASTVAGKKEGKGVVLEGDGFTETGKKPTPLAAAGAAVAQGVPQHIFPAFHAPLPIDMRHQEGRYHYEPHSIHAIHGPPPLSGSPVISDISLIRLSPHPAGPGESPFSPPHPYVTPHMEHYLRSVHGSPTLSVISAARGLSPADVAHEHLKERGLFGLPPPPPGANPTDYYHQMTLMAGHPNPYGDILMQSGGAAGTAHLHEYLSPVDVSRFSSPRVTPRLSRKRALSISPLSDASIDLQTMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGTISPAFSFPHPINPVTYQQILTQQRGLSSAFGHTPPLIQPSPTFPPRQHMAVISVNPPPAQISSNSNCISDSSQEHLTDLKEDLDKDECKQEPEVIYETNCHWEGCTKEYDTQEQLVHHINNDHIHGEKKEFVCRWQDCTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHPRPPPLKENGDNEASAKQSSKVSEESPEANSTTRSMEDCLQVKTIKTENSVMCQSSPGGQSSCSSEPSPLGSTNNNDSGVEMNMHSGGSLGDLTALDDNAPVVDSTVSSGNLTVSLQLRKHMTTMQRLEQLKKEKLKTVKDSCSWVNPAPQGRNTKLPPISGNGSILENSGGSSATLPNPRIMELSVNEVTMLNQINERRDSTTSTISSAYTVSRRSSGISPYFSSRRSSEASQLGHRPNNTSSADSYDPISTDASRRSSEASQCSGMPGLLNLTPAQHYRLKAKYAAATGGPPPTPLPNMERMTLKNRISLMDGPEPTLPSIRLPPGPRRCSDGNTYGYTSTAAFPHEVPGNCTRRASDPVRRPAGEPQALPRVHRFNSTNSMNPFHPPHPADRRNFSMQSYGRSDGSLARHTYSPRPLSISENVTMEAMSGEAEVPVGDDDIMLPDDVVQYIKSQNNGTAAESTSIGYSNEMQSFQGGGKLQPPAFPSQRRMAVADASMSHLGPMMAECPMSFDAPSDMNKNNMPVQWNEVSSGTVDLMSNQSKQQFSQGNLAVVQQKQNFGQYQSYNQQQMQLPENSMNVTQQSFMQRNVGMDGQRLNCMQLRQQPMSLGPGMNPDLGSQAGYNQAHQMLSPSAINGNPNQLSPSCSNMVTKSGPHLHPQQMDMATDPSLMVSSNSERTMLNQVMHEPSQQNYSSQPTHMNFPMAQEAFHQPIMPSNQQSFEPQQSMMGSAAQTYPPGMVQPRPPAEPSPANRPRGLRTVQQLGYMRTPHPTNTMSPGQEAVEAVHKRTGDVLPASTQQCADGTRENNLMYYYGQIHMYEQNSGFDNHSDCRVRQQQCALSGKPAALPSPGTNQVSSTVDSQGLEPPQIDFDAIMDDGDHSSLMSGTLSPSILQNLSQNSSRLTTPRNSLTLPTIPTGISNMAIGDMSSMLTTLAEESKFLNMMS

Target ORF information:

RefSeq Version	XM_015289787.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289787.1

ORF Insert Sequence:

ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA 
GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC 
ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG 
CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT 
CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC 
ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC 
AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG 
CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG 
GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG 
GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG 
ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT 
GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC 
AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT 
GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG 
AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT 
CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC 
AACAGCAACT GCATCTCCGA CTCCAGTCAG GAGCATCTGA CAGACCTGAA AGAAGATCTA 
GATAAGGATG AATGTAAACA GGAGCCTGAG GTTATTTATG AGACTAACTG TCACTGGGAA 
GGGTGCACAA AGGAATATGA CACTCAAGAG CAGCTCGTCC ATCACATCAA CAATGATCAT 
ATCCATGGGG AGAAGAAGGA GTTTGTCTGC CGCTGGCAGG ACTGCACGCG GGAGCAGAAG 
CCCTTCAAAG CTCAATACAT GCTGGTGGTG CACATGCGAA GGCACACTGG AGAGAAGCCC 
CACAAGTGCA CGTTTGAGGG TTGCTCCAAG GCTTACTCCC GCCTGGAGAA CTTAAAGACA 
CACCTGAGGT CCCACACTGG AGAAAAACCT TACGTCTGTG AACACGAAGG CTGCAATAAA 
GCCTTTTCCA ATGCCTCGGA TAGAGCCAAG CACCAGAACA GGACGCATTC CAATGAGAAA 
CCCTATGTCT GTAAGATCCC TGGCTGCACA AAGAGGTACA CAGACCCTAG TTCCCTCAGG 
AAACACGTCA AGACCGTACA CGGACCTGAT GCCCACGTCA CAAAGAAACA GCGCAACGAT 
GTCCACCCAA GGCCGCCTCC GCTCAAGGAA AATGGGGACA ACGAGGCGAG CGCCAAGCAG 
AGCAGTAAGG TGTCAGAGGA GAGCCCCGAG GCCAACAGTA CCACGAGGAG CATGGAGGAT 
TGCTTACAAG TCAAAACGAT AAAAACAGAG AATTCTGTGA TGTGTCAGTC CAGTCCTGGT 
GGCCAGTCAT CATGCAGCAG TGAGCCGTCA CCCCTGGGCA GCACCAACAA CAATGACAGT 
GGAGTAGAAA TGAACATGCA CAGCGGGGGA AGTCTGGGAG ATCTGACGGC GTTGGATGAC 
AACGCTCCTG TTGTGGACTC AACGGTCTCA TCTGGTAACT TGACAGTCAG CCTGCAGCTG 
AGGAAACACA TGACGACAAT GCAACGACTT GAACAGCTCA AGAAAGAGAA ACTCAAGACA 
GTTAAGGATT CCTGCTCATG GGTGAACCCA GCTCCACAAG GCAGAAACAC CAAGCTGCCT 
CCCATTTCAG GAAATGGCTC TATTCTAGAA AACAGTGGTG GTTCTTCTGC TACGCTGCCT 
AACCCAAGAA TAATGGAGCT GTCTGTCAAT GAGGTTACGA TGCTGAACCA AATCAATGAG 
CGCCGTGATA GTACGACAAG CACCATCAGC TCTGCCTACA CCGTGAGCCG CAGATCATCA 
GGGATTTCCC CATACTTCTC CAGCCGCCGC TCCAGCGAGG CTTCACAGCT CGGGCACCGT 
CCCAATAACA CGAGCTCTGC TGACTCTTAT GATCCGATTT CAACGGATGC TTCCCGCCGG 
TCAAGTGAGG CGAGCCAGTG CAGCGGGATG CCGGGCCTAC TGAACCTCAC ACCAGCTCAG 
CACTACAGGC TGAAAGCCAA GTATGCTGCT GCCACAGGGG GCCCTCCTCC AACGCCCCTT 
CCAAACATGG AGAGGATGAC CCTGAAGAAC AGAATCTCAC TTATGGATGG ACCAGAGCCC 
ACCTTGCCCT CCATTCGCCT CCCACCAGGC CCCAGGCGTT GCAGTGATGG CAACACCTAT 
GGCTACACAT CCACCGCAGC ATTTCCCCAT GAGGTTCCGG GCAACTGCAC GAGACGGGCG 
AGCGACCCTG TGAGGAGACC TGCTGGAGAG CCCCAGGCCC TCCCGAGAGT TCACCGCTTC 
AACAGCACCA ACAGCATGAA CCCCTTCCAT CCTCCACATC CTGCAGACCG GAGGAATTTC 
AGCATGCAGA GCTATGGGCG CTCTGACGGG AGTCTTGCCC GGCACACCTA CTCACCCCGG 
CCACTGAGCA TCAGTGAAAA CGTCACTATG GAAGCCATGT CTGGGGAGGC AGAGGTGCCT 
GTTGGAGATG ATGACATTAT GCTGCCAGAT GATGTGGTGC AGTACATCAA ATCTCAGAAC 
AACGGGACAG CAGCTGAGAG CACTTCCATC GGGTACAGCA ATGAGATGCA GAGCTTCCAG 
GGAGGCGGGA AGCTGCAGCC CCCAGCCTTT CCCAGCCAGC GCAGGATGGC GGTGGCCGAC 
GCGAGCATGA GCCATTTGGG GCCCATGATG GCAGAGTGTC CCATGAGCTT TGACGCGCCC 
TCAGACATGA ATAAAAATAA CATGCCTGTC CAGTGGAACG AAGTAAGCTC GGGTACAGTT 
GATCTCATGT CCAATCAATC CAAGCAGCAG TTTTCGCAAG GGAACTTAGC AGTGGTCCAG 
CAGAAGCAGA ACTTTGGTCA GTACCAGAGC TACAACCAGC AGCAGATGCA GCTACCAGAG 
AACAGCATGA ATGTAACACA GCAGAGCTTT ATGCAAAGAA ATGTGGGCAT GGATGGGCAG 
AGGCTAAACT GTATGCAGCT GCGGCAGCAG CCAATGAGCC TAGGCCCAGG GATGAACCCT 
GATCTGGGCT CGCAGGCAGG GTATAACCAA GCTCATCAGA TGCTGAGCCC CAGTGCAATC 
AATGGCAACC CAAATCAGCT CTCTCCTTCT TGTAGCAACA TGGTGACAAA GTCTGGGCCC 
CACCTCCACC CTCAGCAAAT GGACATGGCG ACCGACCCAT CCTTAATGGT CAGCAGCAAC 
AGCGAGCGCA CAATGCTGAA CCAGGTCATG CACGAACCGA GCCAGCAGAA CTACTCATCT 
CAGCCAACCC ACATGAACTT CCCCATGGCT CAGGAGGCTT TCCATCAGCC CATCATGCCC 
TCCAACCAGC AGAGCTTTGA GCCCCAGCAG AGCATGATGG GTTCGGCGGC GCAGACGTAT 
CCACCCGGCA TGGTCCAACC TCGTCCCCCC GCTGAGCCCA GCCCGGCCAA CAGGCCTCGA 
GGCCTCCGCA CCGTGCAGCA GCTGGGCTAC ATGAGAACTC CCCACCCTAC AAACACCATG 
AGCCCGGGCC AGGAGGCGGT GGAGGCTGTG CATAAAAGAA CCGGCGACGT GTTGCCAGCA 
TCGACCCAAC AGTGCGCGGA TGGCACGAGG GAAAATAATT TGATGTACTA TTATGGTCAA 
ATCCACATGT ACGAACAAAA CAGCGGCTTT GATAATCACT CGGACTGCAG GGTCAGGCAG 
CAGCAGTGCG CACTGAGCGG CAAGCCGGCC GCTCTGCCAT CCCCGGGCAC CAACCAGGTG 
TCCAGCACGG TGGACTCACA AGGTCTTGAG CCGCCCCAGA TAGATTTTGA TGCCATCATG 
GACGATGGGG ACCACTCGAG CCTGATGTCA GGAACCCTGA GCCCCAGCAT CCTGCAGAAC 
CTCTCCCAGA ACTCCTCTCG CCTGACGACT CCACGAAACT CTCTGACTCT GCCCACCATA 
CCCACGGGAA TAAGCAATAT GGCAATAGGA GATATGAGCT CCATGCTGAC CACCCTGGCA 
GAAGAGAGTA AATTTCTCAA CATGATGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( NM_001271902.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001258831.1 Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant 2, mRNA.

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CloneID	OGa00089
Clone ID Related Accession (Same CDS sequence)	NM_001271902.1
Accession Version	NM_001271902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Gallus gallus(chicken)
Product	zinc finger protein GLI2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF022818.1, AADN05000350.1 and AM262746.1. Transcript Variant: This variant (2) differs in the 5' UTR, and uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcripts and genomic sequence data because no single transcript from the same breed was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## Transcript exon combination :: AF022818.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201374, SAMEA2201375 [ECO:0000348] ##Evidence-Data-END##

ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGTGCC GCAGCACATT 
TTTCCGGCCT TCCACGCTCC CTTGCCCATT GACATGCGCC ACCAGGAAGG ACGATACCAT 
TACGAGCCTC ACTCTATCCA CGCTATCCAC GGGCCTCCTC CATTGAGCGG CAGCCCCGTC 
ATCTCTGACA TCTCCCTCAT CCGTCTGTCT CCTCATCCTG CCGGGCCTGG CGAATCACCC 
TTCAGCCCAC CGCACCCCTA CGTGACGCCC CACATGGAGC ACTACCTCCG CTCTGTCCAC 
GGCAGCCCCA CGCTCTCCGT CATCTCCGCT GCCAGAGGGC TCAGCCCCGC CGATGTGGCT 
CATGAGCATC TGAAGGAGCG AGGCCTCTTC GGGCTGCCCC CACCGCCACC GGGAGCCAAC 
CCAACCGACT ACTACCACCA AATGACGCTG ATGGCCGGCC ACCCCAACCC CTACGGGGAC 
ATCCTGATGC AGAGCGGCGG AGCAGCCGGC ACGGCCCACC TGCACGAGTA CCTCAGCCCT 
GTTGATGTGT CCCGGTTTTC AAGCCCACGG GTGACTCCGA GGTTAAGCCG AAAACGAGCC 
CTGTCTATCT CCCCGCTGTC TGATGCCAGC ATTGATCTGC AGACGATGAT CAGGACCTCC 
CCAAACTCCC TCGTGGCATA TATTAACAAC TCCAGAAGCA GCTCAGCGGC AAGTGGCTCC 
TACGGCCATT TATCTGCCGG GACAATAAGC CCTGCATTCA GCTTCCCCCA CCCCATCAAC 
CCCGTGACGT ACCAGCAGAT CCTGACCCAG CAGAGAGGTC TGAGCTCAGC TTTCGGGCAC 
ACTCCTCCCC TCATCCAGCC GTCTCCCACC TTTCCCCCTC GGCAGCACAT GGCCGTCATC 
TCCGTCAACC CACCTCCGGC ACAGATCAGC AGCAACAGCA ACTGCATCTC CGACTCCAGT 
CAGAGCAAGC AGAGCAGCGA GTCAGCCGTG AGCAGCACCG TCAATCCCGT AATTAACAAG 
CGCACCAAAG TCAAGACCGA AGTCGAGGGC TTGCCCCCAG TATCCCCAAC CACGCAGGAG 
CATCTGACAG ACCTGAAAGA AGATCTAGAT AAGGATGAAT GTAAACAGGA GCCTGAGGTT 
ATTTATGAGA CTAACTGTCA CTGGGAAGGG TGCACAAAGG AATATGACAC TCAAGAGCAG 
CTCGTCCATC ACATCAACAA TGATCATATC CATGGGGAGA AGAAGGAGTT TGTCTGCCGC 
TGGCAGGACT GCACGCGGGA GCAGAAGCCC TTCAAAGCTC AATACATGCT GGTGGTGCAC 
ATGCGAAGGC ACACTGGAGA GAAGCCCCAC AAGTGCACGT TTGAGGGTTG CTCCAAGGCT 
TACTCCCGCC TGGAGAACTT AAAGACACAC CTGAGGTCCC ACACTGGAGA AAAACCTTAC 
GTCTGTGAAC ACGAAGGCTG CAATAAAGCC TTTTCCAATG CCTCGGATAG AGCCAAGCAC 
CAGAACAGGA CGCATTCCAA TGAGAAACCC TATGTCTGTA AGATCCCTGG CTGCACAAAG 
AGGTACACAG ACCCTAGTTC CCTCAGGAAA CACGTCAAGA CCGTACACGG ACCTGATGCC 
CACGTCACAA AGAAACAGCG CAACGATGTC CACCCAAGGC CGCCTCCGCT CAAGGAAAAT 
GGGGACAACG AGGCGAGCGC CAAGCAGAGC AGTAAGGTGT CAGAGGAGAG CCCCGAGGCC 
AACAGTACCA CGAGGAGCAT GGAGGATTGC TTACAAGTCA AAACGATAAA AACAGAGAAT 
TCTGTGATGT GTCAGTCCAG TCCTGGTGGC CAGTCATCAT GCAGCAGTGA GCCGTCACCC 
CTGGGCAGCA CCAACAACAA TGACAGTGGA GTAGAAATGA ACATGCACAG CGGGGGAAGT 
CTGGGAGATC TGACGGCGTT GGATGACAAC GCTCCTGTTG TGGACTCAAC GGTCTCATCT 
GGTAACTTGA CAGTCAGCCT GCAGCTGAGG AAACACATGA CGACAATGCA ACGACTTGAA 
CAGCTCAAGA AAGAGAAACT CAAGACAGTT AAGGATTCCT GCTCATGGGT GAACCCAGCT 
CCACAAGGCA GAAACACCAA GCTGCCTCCC ATTTCAGGAA ATGGCTCTAT TCTAGAAAAC 
AGTGGTGGTT CTTCTGCTAC GCTGCCTAAC CCAAGAATAA TGGAGCTGTC TGTCAATGAG 
GTTACGATGC TGAACCAAAT CAATGAGCGC CGTGATAGTA CGACAAGCAC CATCAGCTCT 
GCCTACACCG TGAGCCGCAG ATCATCAGGG ATTTCCCCAT ACTTCTCCAG CCGCCGCTCC 
AGCGAGGCTT CACAGCTCGG GCACCGTCCC AATAACACGA GCTCTGCTGA CTCTTATGAT 
CCGATTTCAA CGGATGCTTC CCGCCGGTCA AGTGAGGCGA GCCAGTGCAG CGGGATGCCG 
GGCCTACTGA ACCTCACACC AGCTCAGCAC TACAGGCTGA AAGCCAAGTA TGCTGCTGCC 
ACAGGGGGCC CTCCTCCAAC GCCCCTTCCA AACATGGAGA GGATGACCCT GAAGAACAGA 
ATCTCACTTA TGGATGGACC AGAGCCCACC TTGCCCTCCA TTCGCCTCCC ACCAGGCCCC 
AGGCGTTGCA GTGATGGCAA CACCTATGGC TACACATCCA CCGCAGCATT TCCCCATGAG 
GTTCCGGGCA ACTGCACGAG ACGGGCGAGC GACCCTGTGA GGAGACCTGC TGGAGAGCCC 
CAGGCCCTCC CGAGAGTTCA CCGCTTCAAC AGCACCAACA GCATGAACCC CTTCCATCCT 
CCACATCCTG CAGACCGGAG GAATTTCAGC ATGCAGAGCT ATGGGCGCTC TGACGGGAGT 
CTTGCCCGGC ACACCTACTC ACCCCGGCCA CTGAGCATCA GTGAAAACGT CACTATGGAA 
GCCATGTCTG GGGAGGCAGA GGTGCCTGTT GGAGATGATG ACATTATGCT GCCAGATGAT 
GTGGTGCAGT ACATCAAATC TCAGAACAAC GGGACAGCAG CTGAGAGCAC TTCCATCGGG 
TACAGCAATG AGATGCAGAG CTTCCAGGGA GGCGGGAAGC TGCAGCCCCC AGCCTTTCCC 
AGCCAGCGCA GGATGGCGGT GGCCGACGCG AGCATGAGCC ATTTGGGGCC CATGATGGCA 
GAGTGTCCCA TGAGCTTTGA CGCGCCCTCA GACATGAATA AAAATAACAT GCCTGTCCAG 
TGGAACGAAG TAAGCTCGGG TACAGTTGAT CTCATGTCCA ATCAATCCAA GCAGCAGTTT 
TCGCAAGGGA ACTTAGCAGT GGTCCAGCAG AAGCAGAACT TTGGTCAGTA CCAGAGCTAC 
AACCAGCAGC AGATGCAGCT ACCAGAGAAC AGCATGAATG TAACACAGCA GAGCTTTATG 
CAAAGAAATG TGGGCATGGA TGGGCAGAGG CTAAACTGTA TGCAGCTGCG GCAGCAGCCA 
ATGAGCCTAG GCCCAGGGAT GAACCCTGAT CTGGGCTCGC AGGCAGGGTA TAACCAAGCT 
CATCAGATGC TGAGCCCCAG TGCAATCAAT GGCAACCCAA ATCAGCTCTC TCCTTCTTGT 
AGCAACATGG TGACAAAGTC TGGGCCCCAC CTCCACCCTC AGCAAATGGA CATGGCGACC 
GACCCATCCT TAATGGTCAG CAGCAACAGC GAGCGCACAA TGCTGAACCA GGTCATGCAC 
GAACCGAGCC AGCAGAACTA CTCATCTCAG CCAACCCACA TGAACTTCCC CATGGCTCAG 
GAGGCTTTCC ATCAGCCCAT CATGCCCTCC AACCAGCAGA GCTTTGAGCC CCAGCAGAGC 
ATGATGGGTT CGGCGGCGCA GACGTATCCA CCCGGCATGG TCCAACCTCG TCCCCCCGCT 
GAGCCCAGCC CGGCCAACAG GCCTCGAGGC CTCCGCACCG TGCAGCAGCT GGGCTACATG 
AGAACTCCCC ACCCTACAAA CACCATGAGC CCGGGCCAGG AGGCGGTGGA GGCTGTGCAT 
AAAAGAACCG GCGACGTGTT GCCAGCATCG ACCCAACAGT GCGCGGATGG CACGAGGGAA 
AATAATTTGA TGTACTATTA TGGTCAAATC CACATGTACG AACAAAACAG CGGCTTTGAT 
AATCACTCGG ACTGCAGGGT CAGGCAGCAG CAGTGCGCAC TGAGCGGCAA GCCGGCCGCT 
CTGCCATCCC CGGGCACCAA CCAGGTGTCC AGCACGGTGG ACTCACAAGG TCTTGAGCCG 
CCCCAGATAG ATTTTGATGC CATCATGGAC GATGGGGACC ACTCGAGCCT GATGTCAGGA 
ACCCTGAGCC CCAGCATCCT GCAGAACCTC TCCCAGAACT CCTCTCGCCT GACGACTCCA 
CGAAACTCTC TGACTCTGCC CACCATACCC ACGGGAATAA GCAATATGGC AATAGGAGAT 
ATGAGCTCCA TGCTGACCAC CCTGGCAGAA GAGAGTAAAT TTCTCAACAT GATGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258831.1
CDS	83..4582
Translation	METSASTVAGKKEGKVPQHIFPAFHAPLPIDMRHQEGRYHYEPHSIHAIHGPPPLSGSPVISDISLIRLSPHPAGPGESPFSPPHPYVTPHMEHYLRSVHGSPTLSVISAARGLSPADVAHEHLKERGLFGLPPPPPGANPTDYYHQMTLMAGHPNPYGDILMQSGGAAGTAHLHEYLSPVDVSRFSSPRVTPRLSRKRALSISPLSDASIDLQTMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGTISPAFSFPHPINPVTYQQILTQQRGLSSAFGHTPPLIQPSPTFPPRQHMAVISVNPPPAQISSNSNCISDSSQSKQSSESAVSSTVNPVINKRTKVKTEVEGLPPVSPTTQEHLTDLKEDLDKDECKQEPEVIYETNCHWEGCTKEYDTQEQLVHHINNDHIHGEKKEFVCRWQDCTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHPRPPPLKENGDNEASAKQSSKVSEESPEANSTTRSMEDCLQVKTIKTENSVMCQSSPGGQSSCSSEPSPLGSTNNNDSGVEMNMHSGGSLGDLTALDDNAPVVDSTVSSGNLTVSLQLRKHMTTMQRLEQLKKEKLKTVKDSCSWVNPAPQGRNTKLPPISGNGSILENSGGSSATLPNPRIMELSVNEVTMLNQINERRDSTTSTISSAYTVSRRSSGISPYFSSRRSSEASQLGHRPNNTSSADSYDPISTDASRRSSEASQCSGMPGLLNLTPAQHYRLKAKYAAATGGPPPTPLPNMERMTLKNRISLMDGPEPTLPSIRLPPGPRRCSDGNTYGYTSTAAFPHEVPGNCTRRASDPVRRPAGEPQALPRVHRFNSTNSMNPFHPPHPADRRNFSMQSYGRSDGSLARHTYSPRPLSISENVTMEAMSGEAEVPVGDDDIMLPDDVVQYIKSQNNGTAAESTSIGYSNEMQSFQGGGKLQPPAFPSQRRMAVADASMSHLGPMMAECPMSFDAPSDMNKNNMPVQWNEVSSGTVDLMSNQSKQQFSQGNLAVVQQKQNFGQYQSYNQQQMQLPENSMNVTQQSFMQRNVGMDGQRLNCMQLRQQPMSLGPGMNPDLGSQAGYNQAHQMLSPSAINGNPNQLSPSCSNMVTKSGPHLHPQQMDMATDPSLMVSSNSERTMLNQVMHEPSQQNYSSQPTHMNFPMAQEAFHQPIMPSNQQSFEPQQSMMGSAAQTYPPGMVQPRPPAEPSPANRPRGLRTVQQLGYMRTPHPTNTMSPGQEAVEAVHKRTGDVLPASTQQCADGTRENNLMYYYGQIHMYEQNSGFDNHSDCRVRQQQCALSGKPAALPSPGTNQVSSTVDSQGLEPPQIDFDAIMDDGDHSSLMSGTLSPSILQNLSQNSSRLTTPRNSLTLPTIPTGISNMAIGDMSSMLTTLAEESKFLNMMS

Target ORF information:

RefSeq Version	NM_001271902.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271902.1

ORF Insert Sequence:

ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGTGCC GCAGCACATT 
TTTCCGGCCT TCCACGCTCC CTTGCCCATT GACATGCGCC ACCAGGAAGG ACGATACCAT 
TACGAGCCTC ACTCTATCCA CGCTATCCAC GGGCCTCCTC CATTGAGCGG CAGCCCCGTC 
ATCTCTGACA TCTCCCTCAT CCGTCTGTCT CCTCATCCTG CCGGGCCTGG CGAATCACCC 
TTCAGCCCAC CGCACCCCTA CGTGACGCCC CACATGGAGC ACTACCTCCG CTCTGTCCAC 
GGCAGCCCCA CGCTCTCCGT CATCTCCGCT GCCAGAGGGC TCAGCCCCGC CGATGTGGCT 
CATGAGCATC TGAAGGAGCG AGGCCTCTTC GGGCTGCCCC CACCGCCACC GGGAGCCAAC 
CCAACCGACT ACTACCACCA AATGACGCTG ATGGCCGGCC ACCCCAACCC CTACGGGGAC 
ATCCTGATGC AGAGCGGCGG AGCAGCCGGC ACGGCCCACC TGCACGAGTA CCTCAGCCCT 
GTTGATGTGT CCCGGTTTTC AAGCCCACGG GTGACTCCGA GGTTAAGCCG AAAACGAGCC 
CTGTCTATCT CCCCGCTGTC TGATGCCAGC ATTGATCTGC AGACGATGAT CAGGACCTCC 
CCAAACTCCC TCGTGGCATA TATTAACAAC TCCAGAAGCA GCTCAGCGGC AAGTGGCTCC 
TACGGCCATT TATCTGCCGG GACAATAAGC CCTGCATTCA GCTTCCCCCA CCCCATCAAC 
CCCGTGACGT ACCAGCAGAT CCTGACCCAG CAGAGAGGTC TGAGCTCAGC TTTCGGGCAC 
ACTCCTCCCC TCATCCAGCC GTCTCCCACC TTTCCCCCTC GGCAGCACAT GGCCGTCATC 
TCCGTCAACC CACCTCCGGC ACAGATCAGC AGCAACAGCA ACTGCATCTC CGACTCCAGT 
CAGAGCAAGC AGAGCAGCGA GTCAGCCGTG AGCAGCACCG TCAATCCCGT AATTAACAAG 
CGCACCAAAG TCAAGACCGA AGTCGAGGGC TTGCCCCCAG TATCCCCAAC CACGCAGGAG 
CATCTGACAG ACCTGAAAGA AGATCTAGAT AAGGATGAAT GTAAACAGGA GCCTGAGGTT 
ATTTATGAGA CTAACTGTCA CTGGGAAGGG TGCACAAAGG AATATGACAC TCAAGAGCAG 
CTCGTCCATC ACATCAACAA TGATCATATC CATGGGGAGA AGAAGGAGTT TGTCTGCCGC 
TGGCAGGACT GCACGCGGGA GCAGAAGCCC TTCAAAGCTC AATACATGCT GGTGGTGCAC 
ATGCGAAGGC ACACTGGAGA GAAGCCCCAC AAGTGCACGT TTGAGGGTTG CTCCAAGGCT 
TACTCCCGCC TGGAGAACTT AAAGACACAC CTGAGGTCCC ACACTGGAGA AAAACCTTAC 
GTCTGTGAAC ACGAAGGCTG CAATAAAGCC TTTTCCAATG CCTCGGATAG AGCCAAGCAC 
CAGAACAGGA CGCATTCCAA TGAGAAACCC TATGTCTGTA AGATCCCTGG CTGCACAAAG 
AGGTACACAG ACCCTAGTTC CCTCAGGAAA CACGTCAAGA CCGTACACGG ACCTGATGCC 
CACGTCACAA AGAAACAGCG CAACGATGTC CACCCAAGGC CGCCTCCGCT CAAGGAAAAT 
GGGGACAACG AGGCGAGCGC CAAGCAGAGC AGTAAGGTGT CAGAGGAGAG CCCCGAGGCC 
AACAGTACCA CGAGGAGCAT GGAGGATTGC TTACAAGTCA AAACGATAAA AACAGAGAAT 
TCTGTGATGT GTCAGTCCAG TCCTGGTGGC CAGTCATCAT GCAGCAGTGA GCCGTCACCC 
CTGGGCAGCA CCAACAACAA TGACAGTGGA GTAGAAATGA ACATGCACAG CGGGGGAAGT 
CTGGGAGATC TGACGGCGTT GGATGACAAC GCTCCTGTTG TGGACTCAAC GGTCTCATCT 
GGTAACTTGA CAGTCAGCCT GCAGCTGAGG AAACACATGA CGACAATGCA ACGACTTGAA 
CAGCTCAAGA AAGAGAAACT CAAGACAGTT AAGGATTCCT GCTCATGGGT GAACCCAGCT 
CCACAAGGCA GAAACACCAA GCTGCCTCCC ATTTCAGGAA ATGGCTCTAT TCTAGAAAAC 
AGTGGTGGTT CTTCTGCTAC GCTGCCTAAC CCAAGAATAA TGGAGCTGTC TGTCAATGAG 
GTTACGATGC TGAACCAAAT CAATGAGCGC CGTGATAGTA CGACAAGCAC CATCAGCTCT 
GCCTACACCG TGAGCCGCAG ATCATCAGGG ATTTCCCCAT ACTTCTCCAG CCGCCGCTCC 
AGCGAGGCTT CACAGCTCGG GCACCGTCCC AATAACACGA GCTCTGCTGA CTCTTATGAT 
CCGATTTCAA CGGATGCTTC CCGCCGGTCA AGTGAGGCGA GCCAGTGCAG CGGGATGCCG 
GGCCTACTGA ACCTCACACC AGCTCAGCAC TACAGGCTGA AAGCCAAGTA TGCTGCTGCC 
ACAGGGGGCC CTCCTCCAAC GCCCCTTCCA AACATGGAGA GGATGACCCT GAAGAACAGA 
ATCTCACTTA TGGATGGACC AGAGCCCACC TTGCCCTCCA TTCGCCTCCC ACCAGGCCCC 
AGGCGTTGCA GTGATGGCAA CACCTATGGC TACACATCCA CCGCAGCATT TCCCCATGAG 
GTTCCGGGCA ACTGCACGAG ACGGGCGAGC GACCCTGTGA GGAGACCTGC TGGAGAGCCC 
CAGGCCCTCC CGAGAGTTCA CCGCTTCAAC AGCACCAACA GCATGAACCC CTTCCATCCT 
CCACATCCTG CAGACCGGAG GAATTTCAGC ATGCAGAGCT ATGGGCGCTC TGACGGGAGT 
CTTGCCCGGC ACACCTACTC ACCCCGGCCA CTGAGCATCA GTGAAAACGT CACTATGGAA 
GCCATGTCTG GGGAGGCAGA GGTGCCTGTT GGAGATGATG ACATTATGCT GCCAGATGAT 
GTGGTGCAGT ACATCAAATC TCAGAACAAC GGGACAGCAG CTGAGAGCAC TTCCATCGGG 
TACAGCAATG AGATGCAGAG CTTCCAGGGA GGCGGGAAGC TGCAGCCCCC AGCCTTTCCC 
AGCCAGCGCA GGATGGCGGT GGCCGACGCG AGCATGAGCC ATTTGGGGCC CATGATGGCA 
GAGTGTCCCA TGAGCTTTGA CGCGCCCTCA GACATGAATA AAAATAACAT GCCTGTCCAG 
TGGAACGAAG TAAGCTCGGG TACAGTTGAT CTCATGTCCA ATCAATCCAA GCAGCAGTTT 
TCGCAAGGGA ACTTAGCAGT GGTCCAGCAG AAGCAGAACT TTGGTCAGTA CCAGAGCTAC 
AACCAGCAGC AGATGCAGCT ACCAGAGAAC AGCATGAATG TAACACAGCA GAGCTTTATG 
CAAAGAAATG TGGGCATGGA TGGGCAGAGG CTAAACTGTA TGCAGCTGCG GCAGCAGCCA 
ATGAGCCTAG GCCCAGGGAT GAACCCTGAT CTGGGCTCGC AGGCAGGGTA TAACCAAGCT 
CATCAGATGC TGAGCCCCAG TGCAATCAAT GGCAACCCAA ATCAGCTCTC TCCTTCTTGT 
AGCAACATGG TGACAAAGTC TGGGCCCCAC CTCCACCCTC AGCAAATGGA CATGGCGACC 
GACCCATCCT TAATGGTCAG CAGCAACAGC GAGCGCACAA TGCTGAACCA GGTCATGCAC 
GAACCGAGCC AGCAGAACTA CTCATCTCAG CCAACCCACA TGAACTTCCC CATGGCTCAG 
GAGGCTTTCC ATCAGCCCAT CATGCCCTCC AACCAGCAGA GCTTTGAGCC CCAGCAGAGC 
ATGATGGGTT CGGCGGCGCA GACGTATCCA CCCGGCATGG TCCAACCTCG TCCCCCCGCT 
GAGCCCAGCC CGGCCAACAG GCCTCGAGGC CTCCGCACCG TGCAGCAGCT GGGCTACATG 
AGAACTCCCC ACCCTACAAA CACCATGAGC CCGGGCCAGG AGGCGGTGGA GGCTGTGCAT 
AAAAGAACCG GCGACGTGTT GCCAGCATCG ACCCAACAGT GCGCGGATGG CACGAGGGAA 
AATAATTTGA TGTACTATTA TGGTCAAATC CACATGTACG AACAAAACAG CGGCTTTGAT 
AATCACTCGG ACTGCAGGGT CAGGCAGCAG CAGTGCGCAC TGAGCGGCAA GCCGGCCGCT 
CTGCCATCCC CGGGCACCAA CCAGGTGTCC AGCACGGTGG ACTCACAAGG TCTTGAGCCG 
CCCCAGATAG ATTTTGATGC CATCATGGAC GATGGGGACC ACTCGAGCCT GATGTCAGGA 
ACCCTGAGCC CCAGCATCCT GCAGAACCTC TCCCAGAACT CCTCTCGCCT GACGACTCCA 
CGAAACTCTC TGACTCTGCC CACCATACCC ACGGGAATAA GCAATATGGC AATAGGAGAT 
ATGAGCTCCA TGCTGACCAC CCTGGCAGAA GAGAGTAAAT TTCTCAACAT GATGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( NM_001271901.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001258830.1 Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant 1, mRNA.

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CloneID	OGa00144
Clone ID Related Accession (Same CDS sequence)	NM_001271901.1 , XM_025152142.1 , XM_015289786.1 , XM_015289786.2
Accession Version	NM_001271901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Gallus gallus(chicken)
Product	zinc finger protein GLI2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AM262746.1, AADN05000350.1 and AF022818.1. On Dec 13, 2012 this sequence version replaced XM_003641584.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcripts of different breeds and genomic sequence data because no single transcript from the same breed was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201374, SAMEA2201375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA 
GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC 
ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG 
CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT 
CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC 
ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC 
AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG 
CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG 
GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG 
GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG 
ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT 
GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC 
AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT 
GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG 
AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT 
CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC 
AACAGCAACT GCATCTCCGA CTCCAGTCAG AGCAAGCAGA GCAGCGAGTC AGCCGTGAGC 
AGCACCGTCA ATCCCGTAAT TAACAAGCGC ACCAAAGTCA AGACCGAAGT CGAGGGCTTG 
CCCCCAGTAT CCCCAACCAC GCAGGAGCAT CTGACAGACC TGAAAGAAGA TCTAGATAAG 
GATGAATGTA AACAGGAGCC TGAGGTTATT TATGAGACTA ACTGTCACTG GGAAGGGTGC 
ACAAAGGAAT ATGACACTCA AGAGCAGCTC GTCCATCACA TCAACAATGA TCATATCCAT 
GGGGAGAAGA AGGAGTTTGT CTGCCGCTGG CAGGACTGCA CGCGGGAGCA GAAGCCCTTC 
AAAGCTCAAT ACATGCTGGT GGTGCACATG CGAAGGCACA CTGGAGAGAA GCCCCACAAG 
TGCACGTTTG AGGGTTGCTC CAAGGCTTAC TCCCGCCTGG AGAACTTAAA GACACACCTG 
AGGTCCCACA CTGGAGAAAA ACCTTACGTC TGTGAACACG AAGGCTGCAA TAAAGCCTTT 
TCCAATGCCT CGGATAGAGC CAAGCACCAG AACAGGACGC ATTCCAATGA GAAACCCTAT 
GTCTGTAAGA TCCCTGGCTG CACAAAGAGG TACACAGACC CTAGTTCCCT CAGGAAACAC 
GTCAAGACCG TACACGGACC TGATGCCCAC GTCACAAAGA AACAGCGCAA CGATGTCCAC 
CCAAGGCCGC CTCCGCTCAA GGAAAATGGG GACAACGAGG CGAGCGCCAA GCAGAGCAGT 
AAGGTGTCAG AGGAGAGCCC CGAGGCCAAC AGTACCACGA GGAGCATGGA GGATTGCTTA 
CAAGTCAAAA CGATAAAAAC AGAGAATTCT GTGATGTGTC AGTCCAGTCC TGGTGGCCAG 
TCATCATGCA GCAGTGAGCC GTCACCCCTG GGCAGCACCA ACAACAATGA CAGTGGAGTA 
GAAATGAACA TGCACAGCGG GGGAAGTCTG GGAGATCTGA CGGCGTTGGA TGACAACGCT 
CCTGTTGTGG ACTCAACGGT CTCATCTGGT AACTTGACAG TCAGCCTGCA GCTGAGGAAA 
CACATGACGA CAATGCAACG ACTTGAACAG CTCAAGAAAG AGAAACTCAA GACAGTTAAG 
GATTCCTGCT CATGGGTGAA CCCAGCTCCA CAAGGCAGAA ACACCAAGCT GCCTCCCATT 
TCAGGAAATG GCTCTATTCT AGAAAACAGT GGTGGTTCTT CTGCTACGCT GCCTAACCCA 
AGAATAATGG AGCTGTCTGT CAATGAGGTT ACGATGCTGA ACCAAATCAA TGAGCGCCGT 
GATAGTACGA CAAGCACCAT CAGCTCTGCC TACACCGTGA GCCGCAGATC ATCAGGGATT 
TCCCCATACT TCTCCAGCCG CCGCTCCAGC GAGGCTTCAC AGCTCGGGCA CCGTCCCAAT 
AACACGAGCT CTGCTGACTC TTATGATCCG ATTTCAACGG ATGCTTCCCG CCGGTCAAGT 
GAGGCGAGCC AGTGCAGCGG GATGCCGGGC CTACTGAACC TCACACCAGC TCAGCACTAC 
AGGCTGAAAG CCAAGTATGC TGCTGCCACA GGGGGCCCTC CTCCAACGCC CCTTCCAAAC 
ATGGAGAGGA TGACCCTGAA GAACAGAATC TCACTTATGG ATGGACCAGA GCCCACCTTG 
CCCTCCATTC GCCTCCCACC AGGCCCCAGG CGTTGCAGTG ATGGCAACAC CTATGGCTAC 
ACATCCACCG CAGCATTTCC CCATGAGGTT CCGGGCAACT GCACGAGACG GGCGAGCGAC 
CCTGTGAGGA GACCTGCTGG AGAGCCCCAG GCCCTCCCGA GAGTTCACCG CTTCAACAGC 
ACCAACAGCA TGAACCCCTT CCATCCTCCA CATCCTGCAG ACCGGAGGAA TTTCAGCATG 
CAGAGCTATG GGCGCTCTGA CGGGAGTCTT GCCCGGCACA CCTACTCACC CCGGCCACTG 
AGCATCAGTG AAAACGTCAC TATGGAAGCC ATGTCTGGGG AGGCAGAGGT GCCTGTTGGA 
GATGATGACA TTATGCTGCC AGATGATGTG GTGCAGTACA TCAAATCTCA GAACAACGGG 
ACAGCAGCTG AGAGCACTTC CATCGGGTAC AGCAATGAGA TGCAGAGCTT CCAGGGAGGC 
GGGAAGCTGC AGCCCCCAGC CTTTCCCAGC CAGCGCAGGA TGGCGGTGGC CGACGCGAGC 
ATGAGCCATT TGGGGCCCAT GATGGCAGAG TGTCCCATGA GCTTTGACGC GCCCTCAGAC 
ATGAATAAAA ATAACATGCC TGTCCAGTGG AACGAAGTAA GCTCGGGTAC AGTTGATCTC 
ATGTCCAATC AATCCAAGCA GCAGTTTTCG CAAGGGAACT TAGCAGTGGT CCAGCAGAAG 
CAGAACTTTG GTCAGTACCA GAGCTACAAC CAGCAGCAGA TGCAGCTACC AGAGAACAGC 
ATGAATGTAA CACAGCAGAG CTTTATGCAA AGAAATGTGG GCATGGATGG GCAGAGGCTA 
AACTGTATGC AGCTGCGGCA GCAGCCAATG AGCCTAGGCC CAGGGATGAA CCCTGATCTG 
GGCTCGCAGG CAGGGTATAA CCAAGCTCAT CAGATGCTGA GCCCCAGTGC AATCAATGGC 
AACCCAAATC AGCTCTCTCC TTCTTGTAGC AACATGGTGA CAAAGTCTGG GCCCCACCTC 
CACCCTCAGC AAATGGACAT GGCGACCGAC CCATCCTTAA TGGTCAGCAG CAACAGCGAG 
CGCACAATGC TGAACCAGGT CATGCACGAA CCGAGCCAGC AGAACTACTC ATCTCAGCCA 
ACCCACATGA ACTTCCCCAT GGCTCAGGAG GCTTTCCATC AGCCCATCAT GCCCTCCAAC 
CAGCAGAGCT TTGAGCCCCA GCAGAGCATG ATGGGTTCGG CGGCGCAGAC GTATCCACCC 
GGCATGGTCC AACCTCGTCC CCCCGCTGAG CCCAGCCCGG CCAACAGGCC TCGAGGCCTC 
CGCACCGTGC AGCAGCTGGG CTACATGAGA ACTCCCCACC CTACAAACAC CATGAGCCCG 
GGCCAGGAGG CGGTGGAGGC TGTGCATAAA AGAACCGGCG ACGTGTTGCC AGCATCGACC 
CAACAGTGCG CGGATGGCAC GAGGGAAAAT AATTTGATGT ACTATTATGG TCAAATCCAC 
ATGTACGAAC AAAACAGCGG CTTTGATAAT CACTCGGACT GCAGGGTCAG GCAGCAGCAG 
TGCGCACTGA GCGGCAAGCC GGCCGCTCTG CCATCCCCGG GCACCAACCA GGTGTCCAGC 
ACGGTGGACT CACAAGGTCT TGAGCCGCCC CAGATAGATT TTGATGCCAT CATGGACGAT 
GGGGACCACT CGAGCCTGAT GTCAGGAACC CTGAGCCCCA GCATCCTGCA GAACCTCTCC 
CAGAACTCCT CTCGCCTGAC GACTCCACGA AACTCTCTGA CTCTGCCCAC CATACCCACG 
GGAATAAGCA ATATGGCAAT AGGAGATATG AGCTCCATGC TGACCACCCT GGCAGAAGAG 
AGTAAATTTC TCAACATGAT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258830.1
CDS	99..4685
Translation	METSASTVAGKKEGKGVVLEGDGFTETGKKPTPLAAAGAAVAQGVPQHIFPAFHAPLPIDMRHQEGRYHYEPHSIHAIHGPPPLSGSPVISDISLIRLSPHPAGPGESPFSPPHPYVTPHMEHYLRSVHGSPTLSVISAARGLSPADVAHEHLKERGLFGLPPPPPGANPTDYYHQMTLMAGHPNPYGDILMQSGGAAGTAHLHEYLSPVDVSRFSSPRVTPRLSRKRALSISPLSDASIDLQTMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGTISPAFSFPHPINPVTYQQILTQQRGLSSAFGHTPPLIQPSPTFPPRQHMAVISVNPPPAQISSNSNCISDSSQSKQSSESAVSSTVNPVINKRTKVKTEVEGLPPVSPTTQEHLTDLKEDLDKDECKQEPEVIYETNCHWEGCTKEYDTQEQLVHHINNDHIHGEKKEFVCRWQDCTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHPRPPPLKENGDNEASAKQSSKVSEESPEANSTTRSMEDCLQVKTIKTENSVMCQSSPGGQSSCSSEPSPLGSTNNNDSGVEMNMHSGGSLGDLTALDDNAPVVDSTVSSGNLTVSLQLRKHMTTMQRLEQLKKEKLKTVKDSCSWVNPAPQGRNTKLPPISGNGSILENSGGSSATLPNPRIMELSVNEVTMLNQINERRDSTTSTISSAYTVSRRSSGISPYFSSRRSSEASQLGHRPNNTSSADSYDPISTDASRRSSEASQCSGMPGLLNLTPAQHYRLKAKYAAATGGPPPTPLPNMERMTLKNRISLMDGPEPTLPSIRLPPGPRRCSDGNTYGYTSTAAFPHEVPGNCTRRASDPVRRPAGEPQALPRVHRFNSTNSMNPFHPPHPADRRNFSMQSYGRSDGSLARHTYSPRPLSISENVTMEAMSGEAEVPVGDDDIMLPDDVVQYIKSQNNGTAAESTSIGYSNEMQSFQGGGKLQPPAFPSQRRMAVADASMSHLGPMMAECPMSFDAPSDMNKNNMPVQWNEVSSGTVDLMSNQSKQQFSQGNLAVVQQKQNFGQYQSYNQQQMQLPENSMNVTQQSFMQRNVGMDGQRLNCMQLRQQPMSLGPGMNPDLGSQAGYNQAHQMLSPSAINGNPNQLSPSCSNMVTKSGPHLHPQQMDMATDPSLMVSSNSERTMLNQVMHEPSQQNYSSQPTHMNFPMAQEAFHQPIMPSNQQSFEPQQSMMGSAAQTYPPGMVQPRPPAEPSPANRPRGLRTVQQLGYMRTPHPTNTMSPGQEAVEAVHKRTGDVLPASTQQCADGTRENNLMYYYGQIHMYEQNSGFDNHSDCRVRQQQCALSGKPAALPSPGTNQVSSTVDSQGLEPPQIDFDAIMDDGDHSSLMSGTLSPSILQNLSQNSSRLTTPRNSLTLPTIPTGISNMAIGDMSSMLTTLAEESKFLNMMS

Target ORF information:

RefSeq Version	NM_001271901.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271901.1

ORF Insert Sequence:

ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA 
GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC 
ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG 
CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT 
CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC 
ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC 
AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG 
CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG 
GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG 
GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG 
ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT 
GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC 
AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT 
GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG 
AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT 
CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC 
AACAGCAACT GCATCTCCGA CTCCAGTCAG AGCAAGCAGA GCAGCGAGTC AGCCGTGAGC 
AGCACCGTCA ATCCCGTAAT TAACAAGCGC ACCAAAGTCA AGACCGAAGT CGAGGGCTTG 
CCCCCAGTAT CCCCAACCAC GCAGGAGCAT CTGACAGACC TGAAAGAAGA TCTAGATAAG 
GATGAATGTA AACAGGAGCC TGAGGTTATT TATGAGACTA ACTGTCACTG GGAAGGGTGC 
ACAAAGGAAT ATGACACTCA AGAGCAGCTC GTCCATCACA TCAACAATGA TCATATCCAT 
GGGGAGAAGA AGGAGTTTGT CTGCCGCTGG CAGGACTGCA CGCGGGAGCA GAAGCCCTTC 
AAAGCTCAAT ACATGCTGGT GGTGCACATG CGAAGGCACA CTGGAGAGAA GCCCCACAAG 
TGCACGTTTG AGGGTTGCTC CAAGGCTTAC TCCCGCCTGG AGAACTTAAA GACACACCTG 
AGGTCCCACA CTGGAGAAAA ACCTTACGTC TGTGAACACG AAGGCTGCAA TAAAGCCTTT 
TCCAATGCCT CGGATAGAGC CAAGCACCAG AACAGGACGC ATTCCAATGA GAAACCCTAT 
GTCTGTAAGA TCCCTGGCTG CACAAAGAGG TACACAGACC CTAGTTCCCT CAGGAAACAC 
GTCAAGACCG TACACGGACC TGATGCCCAC GTCACAAAGA AACAGCGCAA CGATGTCCAC 
CCAAGGCCGC CTCCGCTCAA GGAAAATGGG GACAACGAGG CGAGCGCCAA GCAGAGCAGT 
AAGGTGTCAG AGGAGAGCCC CGAGGCCAAC AGTACCACGA GGAGCATGGA GGATTGCTTA 
CAAGTCAAAA CGATAAAAAC AGAGAATTCT GTGATGTGTC AGTCCAGTCC TGGTGGCCAG 
TCATCATGCA GCAGTGAGCC GTCACCCCTG GGCAGCACCA ACAACAATGA CAGTGGAGTA 
GAAATGAACA TGCACAGCGG GGGAAGTCTG GGAGATCTGA CGGCGTTGGA TGACAACGCT 
CCTGTTGTGG ACTCAACGGT CTCATCTGGT AACTTGACAG TCAGCCTGCA GCTGAGGAAA 
CACATGACGA CAATGCAACG ACTTGAACAG CTCAAGAAAG AGAAACTCAA GACAGTTAAG 
GATTCCTGCT CATGGGTGAA CCCAGCTCCA CAAGGCAGAA ACACCAAGCT GCCTCCCATT 
TCAGGAAATG GCTCTATTCT AGAAAACAGT GGTGGTTCTT CTGCTACGCT GCCTAACCCA 
AGAATAATGG AGCTGTCTGT CAATGAGGTT ACGATGCTGA ACCAAATCAA TGAGCGCCGT 
GATAGTACGA CAAGCACCAT CAGCTCTGCC TACACCGTGA GCCGCAGATC ATCAGGGATT 
TCCCCATACT TCTCCAGCCG CCGCTCCAGC GAGGCTTCAC AGCTCGGGCA CCGTCCCAAT 
AACACGAGCT CTGCTGACTC TTATGATCCG ATTTCAACGG ATGCTTCCCG CCGGTCAAGT 
GAGGCGAGCC AGTGCAGCGG GATGCCGGGC CTACTGAACC TCACACCAGC TCAGCACTAC 
AGGCTGAAAG CCAAGTATGC TGCTGCCACA GGGGGCCCTC CTCCAACGCC CCTTCCAAAC 
ATGGAGAGGA TGACCCTGAA GAACAGAATC TCACTTATGG ATGGACCAGA GCCCACCTTG 
CCCTCCATTC GCCTCCCACC AGGCCCCAGG CGTTGCAGTG ATGGCAACAC CTATGGCTAC 
ACATCCACCG CAGCATTTCC CCATGAGGTT CCGGGCAACT GCACGAGACG GGCGAGCGAC 
CCTGTGAGGA GACCTGCTGG AGAGCCCCAG GCCCTCCCGA GAGTTCACCG CTTCAACAGC 
ACCAACAGCA TGAACCCCTT CCATCCTCCA CATCCTGCAG ACCGGAGGAA TTTCAGCATG 
CAGAGCTATG GGCGCTCTGA CGGGAGTCTT GCCCGGCACA CCTACTCACC CCGGCCACTG 
AGCATCAGTG AAAACGTCAC TATGGAAGCC ATGTCTGGGG AGGCAGAGGT GCCTGTTGGA 
GATGATGACA TTATGCTGCC AGATGATGTG GTGCAGTACA TCAAATCTCA GAACAACGGG 
ACAGCAGCTG AGAGCACTTC CATCGGGTAC AGCAATGAGA TGCAGAGCTT CCAGGGAGGC 
GGGAAGCTGC AGCCCCCAGC CTTTCCCAGC CAGCGCAGGA TGGCGGTGGC CGACGCGAGC 
ATGAGCCATT TGGGGCCCAT GATGGCAGAG TGTCCCATGA GCTTTGACGC GCCCTCAGAC 
ATGAATAAAA ATAACATGCC TGTCCAGTGG AACGAAGTAA GCTCGGGTAC AGTTGATCTC 
ATGTCCAATC AATCCAAGCA GCAGTTTTCG CAAGGGAACT TAGCAGTGGT CCAGCAGAAG 
CAGAACTTTG GTCAGTACCA GAGCTACAAC CAGCAGCAGA TGCAGCTACC AGAGAACAGC 
ATGAATGTAA CACAGCAGAG CTTTATGCAA AGAAATGTGG GCATGGATGG GCAGAGGCTA 
AACTGTATGC AGCTGCGGCA GCAGCCAATG AGCCTAGGCC CAGGGATGAA CCCTGATCTG 
GGCTCGCAGG CAGGGTATAA CCAAGCTCAT CAGATGCTGA GCCCCAGTGC AATCAATGGC 
AACCCAAATC AGCTCTCTCC TTCTTGTAGC AACATGGTGA CAAAGTCTGG GCCCCACCTC 
CACCCTCAGC AAATGGACAT GGCGACCGAC CCATCCTTAA TGGTCAGCAG CAACAGCGAG 
CGCACAATGC TGAACCAGGT CATGCACGAA CCGAGCCAGC AGAACTACTC ATCTCAGCCA 
ACCCACATGA ACTTCCCCAT GGCTCAGGAG GCTTTCCATC AGCCCATCAT GCCCTCCAAC 
CAGCAGAGCT TTGAGCCCCA GCAGAGCATG ATGGGTTCGG CGGCGCAGAC GTATCCACCC 
GGCATGGTCC AACCTCGTCC CCCCGCTGAG CCCAGCCCGG CCAACAGGCC TCGAGGCCTC 
CGCACCGTGC AGCAGCTGGG CTACATGAGA ACTCCCCACC CTACAAACAC CATGAGCCCG 
GGCCAGGAGG CGGTGGAGGC TGTGCATAAA AGAACCGGCG ACGTGTTGCC AGCATCGACC 
CAACAGTGCG CGGATGGCAC GAGGGAAAAT AATTTGATGT ACTATTATGG TCAAATCCAC 
ATGTACGAAC AAAACAGCGG CTTTGATAAT CACTCGGACT GCAGGGTCAG GCAGCAGCAG 
TGCGCACTGA GCGGCAAGCC GGCCGCTCTG CCATCCCCGG GCACCAACCA GGTGTCCAGC 
ACGGTGGACT CACAAGGTCT TGAGCCGCCC CAGATAGATT TTGATGCCAT CATGGACGAT 
GGGGACCACT CGAGCCTGAT GTCAGGAACC CTGAGCCCCA GCATCCTGCA GAACCTCTCC 
CAGAACTCCT CTCGCCTGAC GACTCCACGA AACTCTCTGA CTCTGCCCAC CATACCCACG 
GGAATAAGCA ATATGGCAAT AGGAGATATG AGCTCCATGC TGACCACCCT GGCAGAAGAG 
AGTAAATTTC TCAACATGAT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( XM_025152142.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025007910.1 Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X2, mRNA.

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CloneID	OGa00144
Clone ID Related Accession (Same CDS sequence)	NM_001271901.1 , XM_025152142.1 , XM_015289786.1 , XM_015289786.2
Accession Version	XM_025152142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	zinc finger protein GLI2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA 
GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC 
ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG 
CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT 
CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC 
ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC 
AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG 
CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG 
GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG 
GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG 
ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT 
GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC 
AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT 
GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG 
AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT 
CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC 
AACAGCAACT GCATCTCCGA CTCCAGTCAG AGCAAGCAGA GCAGCGAGTC AGCCGTGAGC 
AGCACCGTCA ATCCCGTAAT TAACAAGCGC ACCAAAGTCA AGACCGAAGT CGAGGGCTTG 
CCCCCAGTAT CCCCAACCAC GCAGGAGCAT CTGACAGACC TGAAAGAAGA TCTAGATAAG 
GATGAATGTA AACAGGAGCC TGAGGTTATT TATGAGACTA ACTGTCACTG GGAAGGGTGC 
ACAAAGGAAT ATGACACTCA AGAGCAGCTC GTCCATCACA TCAACAATGA TCATATCCAT 
GGGGAGAAGA AGGAGTTTGT CTGCCGCTGG CAGGACTGCA CGCGGGAGCA GAAGCCCTTC 
AAAGCTCAAT ACATGCTGGT GGTGCACATG CGAAGGCACA CTGGAGAGAA GCCCCACAAG 
TGCACGTTTG AGGGTTGCTC CAAGGCTTAC TCCCGCCTGG AGAACTTAAA GACACACCTG 
AGGTCCCACA CTGGAGAAAA ACCTTACGTC TGTGAACACG AAGGCTGCAA TAAAGCCTTT 
TCCAATGCCT CGGATAGAGC CAAGCACCAG AACAGGACGC ATTCCAATGA GAAACCCTAT 
GTCTGTAAGA TCCCTGGCTG CACAAAGAGG TACACAGACC CTAGTTCCCT CAGGAAACAC 
GTCAAGACCG TACACGGACC TGATGCCCAC GTCACAAAGA AACAGCGCAA CGATGTCCAC 
CCAAGGCCGC CTCCGCTCAA GGAAAATGGG GACAACGAGG CGAGCGCCAA GCAGAGCAGT 
AAGGTGTCAG AGGAGAGCCC CGAGGCCAAC AGTACCACGA GGAGCATGGA GGATTGCTTA 
CAAGTCAAAA CGATAAAAAC AGAGAATTCT GTGATGTGTC AGTCCAGTCC TGGTGGCCAG 
TCATCATGCA GCAGTGAGCC GTCACCCCTG GGCAGCACCA ACAACAATGA CAGTGGAGTA 
GAAATGAACA TGCACAGCGG GGGAAGTCTG GGAGATCTGA CGGCGTTGGA TGACAACGCT 
CCTGTTGTGG ACTCAACGGT CTCATCTGGT AACTTGACAG TCAGCCTGCA GCTGAGGAAA 
CACATGACGA CAATGCAACG ACTTGAACAG CTCAAGAAAG AGAAACTCAA GACAGTTAAG 
GATTCCTGCT CATGGGTGAA CCCAGCTCCA CAAGGCAGAA ACACCAAGCT GCCTCCCATT 
TCAGGAAATG GCTCTATTCT AGAAAACAGT GGTGGTTCTT CTGCTACGCT GCCTAACCCA 
AGAATAATGG AGCTGTCTGT CAATGAGGTT ACGATGCTGA ACCAAATCAA TGAGCGCCGT 
GATAGTACGA CAAGCACCAT CAGCTCTGCC TACACCGTGA GCCGCAGATC ATCAGGGATT 
TCCCCATACT TCTCCAGCCG CCGCTCCAGC GAGGCTTCAC AGCTCGGGCA CCGTCCCAAT 
AACACGAGCT CTGCTGACTC TTATGATCCG ATTTCAACGG ATGCTTCCCG CCGGTCAAGT 
GAGGCGAGCC AGTGCAGCGG GATGCCGGGC CTACTGAACC TCACACCAGC TCAGCACTAC 
AGGCTGAAAG CCAAGTATGC TGCTGCCACA GGGGGCCCTC CTCCAACGCC CCTTCCAAAC 
ATGGAGAGGA TGACCCTGAA GAACAGAATC TCACTTATGG ATGGACCAGA GCCCACCTTG 
CCCTCCATTC GCCTCCCACC AGGCCCCAGG CGTTGCAGTG ATGGCAACAC CTATGGCTAC 
ACATCCACCG CAGCATTTCC CCATGAGGTT CCGGGCAACT GCACGAGACG GGCGAGCGAC 
CCTGTGAGGA GACCTGCTGG AGAGCCCCAG GCCCTCCCGA GAGTTCACCG CTTCAACAGC 
ACCAACAGCA TGAACCCCTT CCATCCTCCA CATCCTGCAG ACCGGAGGAA TTTCAGCATG 
CAGAGCTATG GGCGCTCTGA CGGGAGTCTT GCCCGGCACA CCTACTCACC CCGGCCACTG 
AGCATCAGTG AAAACGTCAC TATGGAAGCC ATGTCTGGGG AGGCAGAGGT GCCTGTTGGA 
GATGATGACA TTATGCTGCC AGATGATGTG GTGCAGTACA TCAAATCTCA GAACAACGGG 
ACAGCAGCTG AGAGCACTTC CATCGGGTAC AGCAATGAGA TGCAGAGCTT CCAGGGAGGC 
GGGAAGCTGC AGCCCCCAGC CTTTCCCAGC CAGCGCAGGA TGGCGGTGGC CGACGCGAGC 
ATGAGCCATT TGGGGCCCAT GATGGCAGAG TGTCCCATGA GCTTTGACGC GCCCTCAGAC 
ATGAATAAAA ATAACATGCC TGTCCAGTGG AACGAAGTAA GCTCGGGTAC AGTTGATCTC 
ATGTCCAATC AATCCAAGCA GCAGTTTTCG CAAGGGAACT TAGCAGTGGT CCAGCAGAAG 
CAGAACTTTG GTCAGTACCA GAGCTACAAC CAGCAGCAGA TGCAGCTACC AGAGAACAGC 
ATGAATGTAA CACAGCAGAG CTTTATGCAA AGAAATGTGG GCATGGATGG GCAGAGGCTA 
AACTGTATGC AGCTGCGGCA GCAGCCAATG AGCCTAGGCC CAGGGATGAA CCCTGATCTG 
GGCTCGCAGG CAGGGTATAA CCAAGCTCAT CAGATGCTGA GCCCCAGTGC AATCAATGGC 
AACCCAAATC AGCTCTCTCC TTCTTGTAGC AACATGGTGA CAAAGTCTGG GCCCCACCTC 
CACCCTCAGC AAATGGACAT GGCGACCGAC CCATCCTTAA TGGTCAGCAG CAACAGCGAG 
CGCACAATGC TGAACCAGGT CATGCACGAA CCGAGCCAGC AGAACTACTC ATCTCAGCCA 
ACCCACATGA ACTTCCCCAT GGCTCAGGAG GCTTTCCATC AGCCCATCAT GCCCTCCAAC 
CAGCAGAGCT TTGAGCCCCA GCAGAGCATG ATGGGTTCGG CGGCGCAGAC GTATCCACCC 
GGCATGGTCC AACCTCGTCC CCCCGCTGAG CCCAGCCCGG CCAACAGGCC TCGAGGCCTC 
CGCACCGTGC AGCAGCTGGG CTACATGAGA ACTCCCCACC CTACAAACAC CATGAGCCCG 
GGCCAGGAGG CGGTGGAGGC TGTGCATAAA AGAACCGGCG ACGTGTTGCC AGCATCGACC 
CAACAGTGCG CGGATGGCAC GAGGGAAAAT AATTTGATGT ACTATTATGG TCAAATCCAC 
ATGTACGAAC AAAACAGCGG CTTTGATAAT CACTCGGACT GCAGGGTCAG GCAGCAGCAG 
TGCGCACTGA GCGGCAAGCC GGCCGCTCTG CCATCCCCGG GCACCAACCA GGTGTCCAGC 
ACGGTGGACT CACAAGGTCT TGAGCCGCCC CAGATAGATT TTGATGCCAT CATGGACGAT 
GGGGACCACT CGAGCCTGAT GTCAGGAACC CTGAGCCCCA GCATCCTGCA GAACCTCTCC 
CAGAACTCCT CTCGCCTGAC GACTCCACGA AACTCTCTGA CTCTGCCCAC CATACCCACG 
GGAATAAGCA ATATGGCAAT AGGAGATATG AGCTCCATGC TGACCACCCT GGCAGAAGAG 
AGTAAATTTC TCAACATGAT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025007910.1
CDS	246..4832
Translation	METSASTVAGKKEGKGVVLEGDGFTETGKKPTPLAAAGAAVAQGVPQHIFPAFHAPLPIDMRHQEGRYHYEPHSIHAIHGPPPLSGSPVISDISLIRLSPHPAGPGESPFSPPHPYVTPHMEHYLRSVHGSPTLSVISAARGLSPADVAHEHLKERGLFGLPPPPPGANPTDYYHQMTLMAGHPNPYGDILMQSGGAAGTAHLHEYLSPVDVSRFSSPRVTPRLSRKRALSISPLSDASIDLQTMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGTISPAFSFPHPINPVTYQQILTQQRGLSSAFGHTPPLIQPSPTFPPRQHMAVISVNPPPAQISSNSNCISDSSQSKQSSESAVSSTVNPVINKRTKVKTEVEGLPPVSPTTQEHLTDLKEDLDKDECKQEPEVIYETNCHWEGCTKEYDTQEQLVHHINNDHIHGEKKEFVCRWQDCTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHPRPPPLKENGDNEASAKQSSKVSEESPEANSTTRSMEDCLQVKTIKTENSVMCQSSPGGQSSCSSEPSPLGSTNNNDSGVEMNMHSGGSLGDLTALDDNAPVVDSTVSSGNLTVSLQLRKHMTTMQRLEQLKKEKLKTVKDSCSWVNPAPQGRNTKLPPISGNGSILENSGGSSATLPNPRIMELSVNEVTMLNQINERRDSTTSTISSAYTVSRRSSGISPYFSSRRSSEASQLGHRPNNTSSADSYDPISTDASRRSSEASQCSGMPGLLNLTPAQHYRLKAKYAAATGGPPPTPLPNMERMTLKNRISLMDGPEPTLPSIRLPPGPRRCSDGNTYGYTSTAAFPHEVPGNCTRRASDPVRRPAGEPQALPRVHRFNSTNSMNPFHPPHPADRRNFSMQSYGRSDGSLARHTYSPRPLSISENVTMEAMSGEAEVPVGDDDIMLPDDVVQYIKSQNNGTAAESTSIGYSNEMQSFQGGGKLQPPAFPSQRRMAVADASMSHLGPMMAECPMSFDAPSDMNKNNMPVQWNEVSSGTVDLMSNQSKQQFSQGNLAVVQQKQNFGQYQSYNQQQMQLPENSMNVTQQSFMQRNVGMDGQRLNCMQLRQQPMSLGPGMNPDLGSQAGYNQAHQMLSPSAINGNPNQLSPSCSNMVTKSGPHLHPQQMDMATDPSLMVSSNSERTMLNQVMHEPSQQNYSSQPTHMNFPMAQEAFHQPIMPSNQQSFEPQQSMMGSAAQTYPPGMVQPRPPAEPSPANRPRGLRTVQQLGYMRTPHPTNTMSPGQEAVEAVHKRTGDVLPASTQQCADGTRENNLMYYYGQIHMYEQNSGFDNHSDCRVRQQQCALSGKPAALPSPGTNQVSSTVDSQGLEPPQIDFDAIMDDGDHSSLMSGTLSPSILQNLSQNSSRLTTPRNSLTLPTIPTGISNMAIGDMSSMLTTLAEESKFLNMMS

Target ORF information:

RefSeq Version	XM_025152142.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025152142.1

ORF Insert Sequence:

ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA 
GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC 
ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG 
CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT 
CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC 
ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC 
AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG 
CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG 
GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG 
GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG 
ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT 
GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC 
AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT 
GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG 
AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT 
CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC 
AACAGCAACT GCATCTCCGA CTCCAGTCAG AGCAAGCAGA GCAGCGAGTC AGCCGTGAGC 
AGCACCGTCA ATCCCGTAAT TAACAAGCGC ACCAAAGTCA AGACCGAAGT CGAGGGCTTG 
CCCCCAGTAT CCCCAACCAC GCAGGAGCAT CTGACAGACC TGAAAGAAGA TCTAGATAAG 
GATGAATGTA AACAGGAGCC TGAGGTTATT TATGAGACTA ACTGTCACTG GGAAGGGTGC 
ACAAAGGAAT ATGACACTCA AGAGCAGCTC GTCCATCACA TCAACAATGA TCATATCCAT 
GGGGAGAAGA AGGAGTTTGT CTGCCGCTGG CAGGACTGCA CGCGGGAGCA GAAGCCCTTC 
AAAGCTCAAT ACATGCTGGT GGTGCACATG CGAAGGCACA CTGGAGAGAA GCCCCACAAG 
TGCACGTTTG AGGGTTGCTC CAAGGCTTAC TCCCGCCTGG AGAACTTAAA GACACACCTG 
AGGTCCCACA CTGGAGAAAA ACCTTACGTC TGTGAACACG AAGGCTGCAA TAAAGCCTTT 
TCCAATGCCT CGGATAGAGC CAAGCACCAG AACAGGACGC ATTCCAATGA GAAACCCTAT 
GTCTGTAAGA TCCCTGGCTG CACAAAGAGG TACACAGACC CTAGTTCCCT CAGGAAACAC 
GTCAAGACCG TACACGGACC TGATGCCCAC GTCACAAAGA AACAGCGCAA CGATGTCCAC 
CCAAGGCCGC CTCCGCTCAA GGAAAATGGG GACAACGAGG CGAGCGCCAA GCAGAGCAGT 
AAGGTGTCAG AGGAGAGCCC CGAGGCCAAC AGTACCACGA GGAGCATGGA GGATTGCTTA 
CAAGTCAAAA CGATAAAAAC AGAGAATTCT GTGATGTGTC AGTCCAGTCC TGGTGGCCAG 
TCATCATGCA GCAGTGAGCC GTCACCCCTG GGCAGCACCA ACAACAATGA CAGTGGAGTA 
GAAATGAACA TGCACAGCGG GGGAAGTCTG GGAGATCTGA CGGCGTTGGA TGACAACGCT 
CCTGTTGTGG ACTCAACGGT CTCATCTGGT AACTTGACAG TCAGCCTGCA GCTGAGGAAA 
CACATGACGA CAATGCAACG ACTTGAACAG CTCAAGAAAG AGAAACTCAA GACAGTTAAG 
GATTCCTGCT CATGGGTGAA CCCAGCTCCA CAAGGCAGAA ACACCAAGCT GCCTCCCATT 
TCAGGAAATG GCTCTATTCT AGAAAACAGT GGTGGTTCTT CTGCTACGCT GCCTAACCCA 
AGAATAATGG AGCTGTCTGT CAATGAGGTT ACGATGCTGA ACCAAATCAA TGAGCGCCGT 
GATAGTACGA CAAGCACCAT CAGCTCTGCC TACACCGTGA GCCGCAGATC ATCAGGGATT 
TCCCCATACT TCTCCAGCCG CCGCTCCAGC GAGGCTTCAC AGCTCGGGCA CCGTCCCAAT 
AACACGAGCT CTGCTGACTC TTATGATCCG ATTTCAACGG ATGCTTCCCG CCGGTCAAGT 
GAGGCGAGCC AGTGCAGCGG GATGCCGGGC CTACTGAACC TCACACCAGC TCAGCACTAC 
AGGCTGAAAG CCAAGTATGC TGCTGCCACA GGGGGCCCTC CTCCAACGCC CCTTCCAAAC 
ATGGAGAGGA TGACCCTGAA GAACAGAATC TCACTTATGG ATGGACCAGA GCCCACCTTG 
CCCTCCATTC GCCTCCCACC AGGCCCCAGG CGTTGCAGTG ATGGCAACAC CTATGGCTAC 
ACATCCACCG CAGCATTTCC CCATGAGGTT CCGGGCAACT GCACGAGACG GGCGAGCGAC 
CCTGTGAGGA GACCTGCTGG AGAGCCCCAG GCCCTCCCGA GAGTTCACCG CTTCAACAGC 
ACCAACAGCA TGAACCCCTT CCATCCTCCA CATCCTGCAG ACCGGAGGAA TTTCAGCATG 
CAGAGCTATG GGCGCTCTGA CGGGAGTCTT GCCCGGCACA CCTACTCACC CCGGCCACTG 
AGCATCAGTG AAAACGTCAC TATGGAAGCC ATGTCTGGGG AGGCAGAGGT GCCTGTTGGA 
GATGATGACA TTATGCTGCC AGATGATGTG GTGCAGTACA TCAAATCTCA GAACAACGGG 
ACAGCAGCTG AGAGCACTTC CATCGGGTAC AGCAATGAGA TGCAGAGCTT CCAGGGAGGC 
GGGAAGCTGC AGCCCCCAGC CTTTCCCAGC CAGCGCAGGA TGGCGGTGGC CGACGCGAGC 
ATGAGCCATT TGGGGCCCAT GATGGCAGAG TGTCCCATGA GCTTTGACGC GCCCTCAGAC 
ATGAATAAAA ATAACATGCC TGTCCAGTGG AACGAAGTAA GCTCGGGTAC AGTTGATCTC 
ATGTCCAATC AATCCAAGCA GCAGTTTTCG CAAGGGAACT TAGCAGTGGT CCAGCAGAAG 
CAGAACTTTG GTCAGTACCA GAGCTACAAC CAGCAGCAGA TGCAGCTACC AGAGAACAGC 
ATGAATGTAA CACAGCAGAG CTTTATGCAA AGAAATGTGG GCATGGATGG GCAGAGGCTA 
AACTGTATGC AGCTGCGGCA GCAGCCAATG AGCCTAGGCC CAGGGATGAA CCCTGATCTG 
GGCTCGCAGG CAGGGTATAA CCAAGCTCAT CAGATGCTGA GCCCCAGTGC AATCAATGGC 
AACCCAAATC AGCTCTCTCC TTCTTGTAGC AACATGGTGA CAAAGTCTGG GCCCCACCTC 
CACCCTCAGC AAATGGACAT GGCGACCGAC CCATCCTTAA TGGTCAGCAG CAACAGCGAG 
CGCACAATGC TGAACCAGGT CATGCACGAA CCGAGCCAGC AGAACTACTC ATCTCAGCCA 
ACCCACATGA ACTTCCCCAT GGCTCAGGAG GCTTTCCATC AGCCCATCAT GCCCTCCAAC 
CAGCAGAGCT TTGAGCCCCA GCAGAGCATG ATGGGTTCGG CGGCGCAGAC GTATCCACCC 
GGCATGGTCC AACCTCGTCC CCCCGCTGAG CCCAGCCCGG CCAACAGGCC TCGAGGCCTC 
CGCACCGTGC AGCAGCTGGG CTACATGAGA ACTCCCCACC CTACAAACAC CATGAGCCCG 
GGCCAGGAGG CGGTGGAGGC TGTGCATAAA AGAACCGGCG ACGTGTTGCC AGCATCGACC 
CAACAGTGCG CGGATGGCAC GAGGGAAAAT AATTTGATGT ACTATTATGG TCAAATCCAC 
ATGTACGAAC AAAACAGCGG CTTTGATAAT CACTCGGACT GCAGGGTCAG GCAGCAGCAG 
TGCGCACTGA GCGGCAAGCC GGCCGCTCTG CCATCCCCGG GCACCAACCA GGTGTCCAGC 
ACGGTGGACT CACAAGGTCT TGAGCCGCCC CAGATAGATT TTGATGCCAT CATGGACGAT 
GGGGACCACT CGAGCCTGAT GTCAGGAACC CTGAGCCCCA GCATCCTGCA GAACCTCTCC 
CAGAACTCCT CTCGCCTGAC GACTCCACGA AACTCTCTGA CTCTGCCCAC CATACCCACG 
GGAATAAGCA ATATGGCAAT AGGAGATATG AGCTCCATGC TGACCACCCT GGCAGAAGAG 
AGTAAATTTC TCAACATGAT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( XM_015289786.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145272.1 Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa00144
Clone ID Related Accession (Same CDS sequence)	NM_001271901.1 , XM_025152142.1 , XM_015289786.1 , XM_015289786.2
Accession Version	XM_015289786.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	zinc finger protein GLI2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA 
GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC 
ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG 
CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT 
CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC 
ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC 
AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG 
CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG 
GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG 
GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG 
ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT 
GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC 
AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT 
GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG 
AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT 
CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC 
AACAGCAACT GCATCTCCGA CTCCAGTCAG AGCAAGCAGA GCAGCGAGTC AGCCGTGAGC 
AGCACCGTCA ATCCCGTAAT TAACAAGCGC ACCAAAGTCA AGACCGAAGT CGAGGGCTTG 
CCCCCAGTAT CCCCAACCAC GCAGGAGCAT CTGACAGACC TGAAAGAAGA TCTAGATAAG 
GATGAATGTA AACAGGAGCC TGAGGTTATT TATGAGACTA ACTGTCACTG GGAAGGGTGC 
ACAAAGGAAT ATGACACTCA AGAGCAGCTC GTCCATCACA TCAACAATGA TCATATCCAT 
GGGGAGAAGA AGGAGTTTGT CTGCCGCTGG CAGGACTGCA CGCGGGAGCA GAAGCCCTTC 
AAAGCTCAAT ACATGCTGGT GGTGCACATG CGAAGGCACA CTGGAGAGAA GCCCCACAAG 
TGCACGTTTG AGGGTTGCTC CAAGGCTTAC TCCCGCCTGG AGAACTTAAA GACACACCTG 
AGGTCCCACA CTGGAGAAAA ACCTTACGTC TGTGAACACG AAGGCTGCAA TAAAGCCTTT 
TCCAATGCCT CGGATAGAGC CAAGCACCAG AACAGGACGC ATTCCAATGA GAAACCCTAT 
GTCTGTAAGA TCCCTGGCTG CACAAAGAGG TACACAGACC CTAGTTCCCT CAGGAAACAC 
GTCAAGACCG TACACGGACC TGATGCCCAC GTCACAAAGA AACAGCGCAA CGATGTCCAC 
CCAAGGCCGC CTCCGCTCAA GGAAAATGGG GACAACGAGG CGAGCGCCAA GCAGAGCAGT 
AAGGTGTCAG AGGAGAGCCC CGAGGCCAAC AGTACCACGA GGAGCATGGA GGATTGCTTA 
CAAGTCAAAA CGATAAAAAC AGAGAATTCT GTGATGTGTC AGTCCAGTCC TGGTGGCCAG 
TCATCATGCA GCAGTGAGCC GTCACCCCTG GGCAGCACCA ACAACAATGA CAGTGGAGTA 
GAAATGAACA TGCACAGCGG GGGAAGTCTG GGAGATCTGA CGGCGTTGGA TGACAACGCT 
CCTGTTGTGG ACTCAACGGT CTCATCTGGT AACTTGACAG TCAGCCTGCA GCTGAGGAAA 
CACATGACGA CAATGCAACG ACTTGAACAG CTCAAGAAAG AGAAACTCAA GACAGTTAAG 
GATTCCTGCT CATGGGTGAA CCCAGCTCCA CAAGGCAGAA ACACCAAGCT GCCTCCCATT 
TCAGGAAATG GCTCTATTCT AGAAAACAGT GGTGGTTCTT CTGCTACGCT GCCTAACCCA 
AGAATAATGG AGCTGTCTGT CAATGAGGTT ACGATGCTGA ACCAAATCAA TGAGCGCCGT 
GATAGTACGA CAAGCACCAT CAGCTCTGCC TACACCGTGA GCCGCAGATC ATCAGGGATT 
TCCCCATACT TCTCCAGCCG CCGCTCCAGC GAGGCTTCAC AGCTCGGGCA CCGTCCCAAT 
AACACGAGCT CTGCTGACTC TTATGATCCG ATTTCAACGG ATGCTTCCCG CCGGTCAAGT 
GAGGCGAGCC AGTGCAGCGG GATGCCGGGC CTACTGAACC TCACACCAGC TCAGCACTAC 
AGGCTGAAAG CCAAGTATGC TGCTGCCACA GGGGGCCCTC CTCCAACGCC CCTTCCAAAC 
ATGGAGAGGA TGACCCTGAA GAACAGAATC TCACTTATGG ATGGACCAGA GCCCACCTTG 
CCCTCCATTC GCCTCCCACC AGGCCCCAGG CGTTGCAGTG ATGGCAACAC CTATGGCTAC 
ACATCCACCG CAGCATTTCC CCATGAGGTT CCGGGCAACT GCACGAGACG GGCGAGCGAC 
CCTGTGAGGA GACCTGCTGG AGAGCCCCAG GCCCTCCCGA GAGTTCACCG CTTCAACAGC 
ACCAACAGCA TGAACCCCTT CCATCCTCCA CATCCTGCAG ACCGGAGGAA TTTCAGCATG 
CAGAGCTATG GGCGCTCTGA CGGGAGTCTT GCCCGGCACA CCTACTCACC CCGGCCACTG 
AGCATCAGTG AAAACGTCAC TATGGAAGCC ATGTCTGGGG AGGCAGAGGT GCCTGTTGGA 
GATGATGACA TTATGCTGCC AGATGATGTG GTGCAGTACA TCAAATCTCA GAACAACGGG 
ACAGCAGCTG AGAGCACTTC CATCGGGTAC AGCAATGAGA TGCAGAGCTT CCAGGGAGGC 
GGGAAGCTGC AGCCCCCAGC CTTTCCCAGC CAGCGCAGGA TGGCGGTGGC CGACGCGAGC 
ATGAGCCATT TGGGGCCCAT GATGGCAGAG TGTCCCATGA GCTTTGACGC GCCCTCAGAC 
ATGAATAAAA ATAACATGCC TGTCCAGTGG AACGAAGTAA GCTCGGGTAC AGTTGATCTC 
ATGTCCAATC AATCCAAGCA GCAGTTTTCG CAAGGGAACT TAGCAGTGGT CCAGCAGAAG 
CAGAACTTTG GTCAGTACCA GAGCTACAAC CAGCAGCAGA TGCAGCTACC AGAGAACAGC 
ATGAATGTAA CACAGCAGAG CTTTATGCAA AGAAATGTGG GCATGGATGG GCAGAGGCTA 
AACTGTATGC AGCTGCGGCA GCAGCCAATG AGCCTAGGCC CAGGGATGAA CCCTGATCTG 
GGCTCGCAGG CAGGGTATAA CCAAGCTCAT CAGATGCTGA GCCCCAGTGC AATCAATGGC 
AACCCAAATC AGCTCTCTCC TTCTTGTAGC AACATGGTGA CAAAGTCTGG GCCCCACCTC 
CACCCTCAGC AAATGGACAT GGCGACCGAC CCATCCTTAA TGGTCAGCAG CAACAGCGAG 
CGCACAATGC TGAACCAGGT CATGCACGAA CCGAGCCAGC AGAACTACTC ATCTCAGCCA 
ACCCACATGA ACTTCCCCAT GGCTCAGGAG GCTTTCCATC AGCCCATCAT GCCCTCCAAC 
CAGCAGAGCT TTGAGCCCCA GCAGAGCATG ATGGGTTCGG CGGCGCAGAC GTATCCACCC 
GGCATGGTCC AACCTCGTCC CCCCGCTGAG CCCAGCCCGG CCAACAGGCC TCGAGGCCTC 
CGCACCGTGC AGCAGCTGGG CTACATGAGA ACTCCCCACC CTACAAACAC CATGAGCCCG 
GGCCAGGAGG CGGTGGAGGC TGTGCATAAA AGAACCGGCG ACGTGTTGCC AGCATCGACC 
CAACAGTGCG CGGATGGCAC GAGGGAAAAT AATTTGATGT ACTATTATGG TCAAATCCAC 
ATGTACGAAC AAAACAGCGG CTTTGATAAT CACTCGGACT GCAGGGTCAG GCAGCAGCAG 
TGCGCACTGA GCGGCAAGCC GGCCGCTCTG CCATCCCCGG GCACCAACCA GGTGTCCAGC 
ACGGTGGACT CACAAGGTCT TGAGCCGCCC CAGATAGATT TTGATGCCAT CATGGACGAT 
GGGGACCACT CGAGCCTGAT GTCAGGAACC CTGAGCCCCA GCATCCTGCA GAACCTCTCC 
CAGAACTCCT CTCGCCTGAC GACTCCACGA AACTCTCTGA CTCTGCCCAC CATACCCACG 
GGAATAAGCA ATATGGCAAT AGGAGATATG AGCTCCATGC TGACCACCCT GGCAGAAGAG 
AGTAAATTTC TCAACATGAT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145272.1
CDS	315..4901
Translation	METSASTVAGKKEGKGVVLEGDGFTETGKKPTPLAAAGAAVAQGVPQHIFPAFHAPLPIDMRHQEGRYHYEPHSIHAIHGPPPLSGSPVISDISLIRLSPHPAGPGESPFSPPHPYVTPHMEHYLRSVHGSPTLSVISAARGLSPADVAHEHLKERGLFGLPPPPPGANPTDYYHQMTLMAGHPNPYGDILMQSGGAAGTAHLHEYLSPVDVSRFSSPRVTPRLSRKRALSISPLSDASIDLQTMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGTISPAFSFPHPINPVTYQQILTQQRGLSSAFGHTPPLIQPSPTFPPRQHMAVISVNPPPAQISSNSNCISDSSQSKQSSESAVSSTVNPVINKRTKVKTEVEGLPPVSPTTQEHLTDLKEDLDKDECKQEPEVIYETNCHWEGCTKEYDTQEQLVHHINNDHIHGEKKEFVCRWQDCTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHPRPPPLKENGDNEASAKQSSKVSEESPEANSTTRSMEDCLQVKTIKTENSVMCQSSPGGQSSCSSEPSPLGSTNNNDSGVEMNMHSGGSLGDLTALDDNAPVVDSTVSSGNLTVSLQLRKHMTTMQRLEQLKKEKLKTVKDSCSWVNPAPQGRNTKLPPISGNGSILENSGGSSATLPNPRIMELSVNEVTMLNQINERRDSTTSTISSAYTVSRRSSGISPYFSSRRSSEASQLGHRPNNTSSADSYDPISTDASRRSSEASQCSGMPGLLNLTPAQHYRLKAKYAAATGGPPPTPLPNMERMTLKNRISLMDGPEPTLPSIRLPPGPRRCSDGNTYGYTSTAAFPHEVPGNCTRRASDPVRRPAGEPQALPRVHRFNSTNSMNPFHPPHPADRRNFSMQSYGRSDGSLARHTYSPRPLSISENVTMEAMSGEAEVPVGDDDIMLPDDVVQYIKSQNNGTAAESTSIGYSNEMQSFQGGGKLQPPAFPSQRRMAVADASMSHLGPMMAECPMSFDAPSDMNKNNMPVQWNEVSSGTVDLMSNQSKQQFSQGNLAVVQQKQNFGQYQSYNQQQMQLPENSMNVTQQSFMQRNVGMDGQRLNCMQLRQQPMSLGPGMNPDLGSQAGYNQAHQMLSPSAINGNPNQLSPSCSNMVTKSGPHLHPQQMDMATDPSLMVSSNSERTMLNQVMHEPSQQNYSSQPTHMNFPMAQEAFHQPIMPSNQQSFEPQQSMMGSAAQTYPPGMVQPRPPAEPSPANRPRGLRTVQQLGYMRTPHPTNTMSPGQEAVEAVHKRTGDVLPASTQQCADGTRENNLMYYYGQIHMYEQNSGFDNHSDCRVRQQQCALSGKPAALPSPGTNQVSSTVDSQGLEPPQIDFDAIMDDGDHSSLMSGTLSPSILQNLSQNSSRLTTPRNSLTLPTIPTGISNMAIGDMSSMLTTLAEESKFLNMMS

Target ORF information:

RefSeq Version	XM_015289786.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289786.1

ORF Insert Sequence:

ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA 
GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC 
ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG 
CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT 
CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC 
ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC 
AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG 
CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG 
GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG 
GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG 
ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT 
GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC 
AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT 
GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG 
AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT 
CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC 
AACAGCAACT GCATCTCCGA CTCCAGTCAG AGCAAGCAGA GCAGCGAGTC AGCCGTGAGC 
AGCACCGTCA ATCCCGTAAT TAACAAGCGC ACCAAAGTCA AGACCGAAGT CGAGGGCTTG 
CCCCCAGTAT CCCCAACCAC GCAGGAGCAT CTGACAGACC TGAAAGAAGA TCTAGATAAG 
GATGAATGTA AACAGGAGCC TGAGGTTATT TATGAGACTA ACTGTCACTG GGAAGGGTGC 
ACAAAGGAAT ATGACACTCA AGAGCAGCTC GTCCATCACA TCAACAATGA TCATATCCAT 
GGGGAGAAGA AGGAGTTTGT CTGCCGCTGG CAGGACTGCA CGCGGGAGCA GAAGCCCTTC 
AAAGCTCAAT ACATGCTGGT GGTGCACATG CGAAGGCACA CTGGAGAGAA GCCCCACAAG 
TGCACGTTTG AGGGTTGCTC CAAGGCTTAC TCCCGCCTGG AGAACTTAAA GACACACCTG 
AGGTCCCACA CTGGAGAAAA ACCTTACGTC TGTGAACACG AAGGCTGCAA TAAAGCCTTT 
TCCAATGCCT CGGATAGAGC CAAGCACCAG AACAGGACGC ATTCCAATGA GAAACCCTAT 
GTCTGTAAGA TCCCTGGCTG CACAAAGAGG TACACAGACC CTAGTTCCCT CAGGAAACAC 
GTCAAGACCG TACACGGACC TGATGCCCAC GTCACAAAGA AACAGCGCAA CGATGTCCAC 
CCAAGGCCGC CTCCGCTCAA GGAAAATGGG GACAACGAGG CGAGCGCCAA GCAGAGCAGT 
AAGGTGTCAG AGGAGAGCCC CGAGGCCAAC AGTACCACGA GGAGCATGGA GGATTGCTTA 
CAAGTCAAAA CGATAAAAAC AGAGAATTCT GTGATGTGTC AGTCCAGTCC TGGTGGCCAG 
TCATCATGCA GCAGTGAGCC GTCACCCCTG GGCAGCACCA ACAACAATGA CAGTGGAGTA 
GAAATGAACA TGCACAGCGG GGGAAGTCTG GGAGATCTGA CGGCGTTGGA TGACAACGCT 
CCTGTTGTGG ACTCAACGGT CTCATCTGGT AACTTGACAG TCAGCCTGCA GCTGAGGAAA 
CACATGACGA CAATGCAACG ACTTGAACAG CTCAAGAAAG AGAAACTCAA GACAGTTAAG 
GATTCCTGCT CATGGGTGAA CCCAGCTCCA CAAGGCAGAA ACACCAAGCT GCCTCCCATT 
TCAGGAAATG GCTCTATTCT AGAAAACAGT GGTGGTTCTT CTGCTACGCT GCCTAACCCA 
AGAATAATGG AGCTGTCTGT CAATGAGGTT ACGATGCTGA ACCAAATCAA TGAGCGCCGT 
GATAGTACGA CAAGCACCAT CAGCTCTGCC TACACCGTGA GCCGCAGATC ATCAGGGATT 
TCCCCATACT TCTCCAGCCG CCGCTCCAGC GAGGCTTCAC AGCTCGGGCA CCGTCCCAAT 
AACACGAGCT CTGCTGACTC TTATGATCCG ATTTCAACGG ATGCTTCCCG CCGGTCAAGT 
GAGGCGAGCC AGTGCAGCGG GATGCCGGGC CTACTGAACC TCACACCAGC TCAGCACTAC 
AGGCTGAAAG CCAAGTATGC TGCTGCCACA GGGGGCCCTC CTCCAACGCC CCTTCCAAAC 
ATGGAGAGGA TGACCCTGAA GAACAGAATC TCACTTATGG ATGGACCAGA GCCCACCTTG 
CCCTCCATTC GCCTCCCACC AGGCCCCAGG CGTTGCAGTG ATGGCAACAC CTATGGCTAC 
ACATCCACCG CAGCATTTCC CCATGAGGTT CCGGGCAACT GCACGAGACG GGCGAGCGAC 
CCTGTGAGGA GACCTGCTGG AGAGCCCCAG GCCCTCCCGA GAGTTCACCG CTTCAACAGC 
ACCAACAGCA TGAACCCCTT CCATCCTCCA CATCCTGCAG ACCGGAGGAA TTTCAGCATG 
CAGAGCTATG GGCGCTCTGA CGGGAGTCTT GCCCGGCACA CCTACTCACC CCGGCCACTG 
AGCATCAGTG AAAACGTCAC TATGGAAGCC ATGTCTGGGG AGGCAGAGGT GCCTGTTGGA 
GATGATGACA TTATGCTGCC AGATGATGTG GTGCAGTACA TCAAATCTCA GAACAACGGG 
ACAGCAGCTG AGAGCACTTC CATCGGGTAC AGCAATGAGA TGCAGAGCTT CCAGGGAGGC 
GGGAAGCTGC AGCCCCCAGC CTTTCCCAGC CAGCGCAGGA TGGCGGTGGC CGACGCGAGC 
ATGAGCCATT TGGGGCCCAT GATGGCAGAG TGTCCCATGA GCTTTGACGC GCCCTCAGAC 
ATGAATAAAA ATAACATGCC TGTCCAGTGG AACGAAGTAA GCTCGGGTAC AGTTGATCTC 
ATGTCCAATC AATCCAAGCA GCAGTTTTCG CAAGGGAACT TAGCAGTGGT CCAGCAGAAG 
CAGAACTTTG GTCAGTACCA GAGCTACAAC CAGCAGCAGA TGCAGCTACC AGAGAACAGC 
ATGAATGTAA CACAGCAGAG CTTTATGCAA AGAAATGTGG GCATGGATGG GCAGAGGCTA 
AACTGTATGC AGCTGCGGCA GCAGCCAATG AGCCTAGGCC CAGGGATGAA CCCTGATCTG 
GGCTCGCAGG CAGGGTATAA CCAAGCTCAT CAGATGCTGA GCCCCAGTGC AATCAATGGC 
AACCCAAATC AGCTCTCTCC TTCTTGTAGC AACATGGTGA CAAAGTCTGG GCCCCACCTC 
CACCCTCAGC AAATGGACAT GGCGACCGAC CCATCCTTAA TGGTCAGCAG CAACAGCGAG 
CGCACAATGC TGAACCAGGT CATGCACGAA CCGAGCCAGC AGAACTACTC ATCTCAGCCA 
ACCCACATGA ACTTCCCCAT GGCTCAGGAG GCTTTCCATC AGCCCATCAT GCCCTCCAAC 
CAGCAGAGCT TTGAGCCCCA GCAGAGCATG ATGGGTTCGG CGGCGCAGAC GTATCCACCC 
GGCATGGTCC AACCTCGTCC CCCCGCTGAG CCCAGCCCGG CCAACAGGCC TCGAGGCCTC 
CGCACCGTGC AGCAGCTGGG CTACATGAGA ACTCCCCACC CTACAAACAC CATGAGCCCG 
GGCCAGGAGG CGGTGGAGGC TGTGCATAAA AGAACCGGCG ACGTGTTGCC AGCATCGACC 
CAACAGTGCG CGGATGGCAC GAGGGAAAAT AATTTGATGT ACTATTATGG TCAAATCCAC 
ATGTACGAAC AAAACAGCGG CTTTGATAAT CACTCGGACT GCAGGGTCAG GCAGCAGCAG 
TGCGCACTGA GCGGCAAGCC GGCCGCTCTG CCATCCCCGG GCACCAACCA GGTGTCCAGC 
ACGGTGGACT CACAAGGTCT TGAGCCGCCC CAGATAGATT TTGATGCCAT CATGGACGAT 
GGGGACCACT CGAGCCTGAT GTCAGGAACC CTGAGCCCCA GCATCCTGCA GAACCTCTCC 
CAGAACTCCT CTCGCCTGAC GACTCCACGA AACTCTCTGA CTCTGCCCAC CATACCCACG 
GGAATAAGCA ATATGGCAAT AGGAGATATG AGCTCCATGC TGACCACCCT GGCAGAAGAG 
AGTAAATTTC TCAACATGAT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI2 ( XM_015289786.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145272.1 Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa00144
Clone ID Related Accession (Same CDS sequence)	NM_001271901.1 , XM_025152142.1 , XM_015289786.1 , XM_015289786.2
Accession Version	XM_015289786.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	zinc finger protein GLI2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289786.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA 
GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC 
ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG 
CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT 
CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC 
ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC 
AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG 
CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG 
GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG 
GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG 
ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT 
GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC 
AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT 
GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG 
AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT 
CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC 
AACAGCAACT GCATCTCCGA CTCCAGTCAG AGCAAGCAGA GCAGCGAGTC AGCCGTGAGC 
AGCACCGTCA ATCCCGTAAT TAACAAGCGC ACCAAAGTCA AGACCGAAGT CGAGGGCTTG 
CCCCCAGTAT CCCCAACCAC GCAGGAGCAT CTGACAGACC TGAAAGAAGA TCTAGATAAG 
GATGAATGTA AACAGGAGCC TGAGGTTATT TATGAGACTA ACTGTCACTG GGAAGGGTGC 
ACAAAGGAAT ATGACACTCA AGAGCAGCTC GTCCATCACA TCAACAATGA TCATATCCAT 
GGGGAGAAGA AGGAGTTTGT CTGCCGCTGG CAGGACTGCA CGCGGGAGCA GAAGCCCTTC 
AAAGCTCAAT ACATGCTGGT GGTGCACATG CGAAGGCACA CTGGAGAGAA GCCCCACAAG 
TGCACGTTTG AGGGTTGCTC CAAGGCTTAC TCCCGCCTGG AGAACTTAAA GACACACCTG 
AGGTCCCACA CTGGAGAAAA ACCTTACGTC TGTGAACACG AAGGCTGCAA TAAAGCCTTT 
TCCAATGCCT CGGATAGAGC CAAGCACCAG AACAGGACGC ATTCCAATGA GAAACCCTAT 
GTCTGTAAGA TCCCTGGCTG CACAAAGAGG TACACAGACC CTAGTTCCCT CAGGAAACAC 
GTCAAGACCG TACACGGACC TGATGCCCAC GTCACAAAGA AACAGCGCAA CGATGTCCAC 
CCAAGGCCGC CTCCGCTCAA GGAAAATGGG GACAACGAGG CGAGCGCCAA GCAGAGCAGT 
AAGGTGTCAG AGGAGAGCCC CGAGGCCAAC AGTACCACGA GGAGCATGGA GGATTGCTTA 
CAAGTCAAAA CGATAAAAAC AGAGAATTCT GTGATGTGTC AGTCCAGTCC TGGTGGCCAG 
TCATCATGCA GCAGTGAGCC GTCACCCCTG GGCAGCACCA ACAACAATGA CAGTGGAGTA 
GAAATGAACA TGCACAGCGG GGGAAGTCTG GGAGATCTGA CGGCGTTGGA TGACAACGCT 
CCTGTTGTGG ACTCAACGGT CTCATCTGGT AACTTGACAG TCAGCCTGCA GCTGAGGAAA 
CACATGACGA CAATGCAACG ACTTGAACAG CTCAAGAAAG AGAAACTCAA GACAGTTAAG 
GATTCCTGCT CATGGGTGAA CCCAGCTCCA CAAGGCAGAA ACACCAAGCT GCCTCCCATT 
TCAGGAAATG GCTCTATTCT AGAAAACAGT GGTGGTTCTT CTGCTACGCT GCCTAACCCA 
AGAATAATGG AGCTGTCTGT CAATGAGGTT ACGATGCTGA ACCAAATCAA TGAGCGCCGT 
GATAGTACGA CAAGCACCAT CAGCTCTGCC TACACCGTGA GCCGCAGATC ATCAGGGATT 
TCCCCATACT TCTCCAGCCG CCGCTCCAGC GAGGCTTCAC AGCTCGGGCA CCGTCCCAAT 
AACACGAGCT CTGCTGACTC TTATGATCCG ATTTCAACGG ATGCTTCCCG CCGGTCAAGT 
GAGGCGAGCC AGTGCAGCGG GATGCCGGGC CTACTGAACC TCACACCAGC TCAGCACTAC 
AGGCTGAAAG CCAAGTATGC TGCTGCCACA GGGGGCCCTC CTCCAACGCC CCTTCCAAAC 
ATGGAGAGGA TGACCCTGAA GAACAGAATC TCACTTATGG ATGGACCAGA GCCCACCTTG 
CCCTCCATTC GCCTCCCACC AGGCCCCAGG CGTTGCAGTG ATGGCAACAC CTATGGCTAC 
ACATCCACCG CAGCATTTCC CCATGAGGTT CCGGGCAACT GCACGAGACG GGCGAGCGAC 
CCTGTGAGGA GACCTGCTGG AGAGCCCCAG GCCCTCCCGA GAGTTCACCG CTTCAACAGC 
ACCAACAGCA TGAACCCCTT CCATCCTCCA CATCCTGCAG ACCGGAGGAA TTTCAGCATG 
CAGAGCTATG GGCGCTCTGA CGGGAGTCTT GCCCGGCACA CCTACTCACC CCGGCCACTG 
AGCATCAGTG AAAACGTCAC TATGGAAGCC ATGTCTGGGG AGGCAGAGGT GCCTGTTGGA 
GATGATGACA TTATGCTGCC AGATGATGTG GTGCAGTACA TCAAATCTCA GAACAACGGG 
ACAGCAGCTG AGAGCACTTC CATCGGGTAC AGCAATGAGA TGCAGAGCTT CCAGGGAGGC 
GGGAAGCTGC AGCCCCCAGC CTTTCCCAGC CAGCGCAGGA TGGCGGTGGC CGACGCGAGC 
ATGAGCCATT TGGGGCCCAT GATGGCAGAG TGTCCCATGA GCTTTGACGC GCCCTCAGAC 
ATGAATAAAA ATAACATGCC TGTCCAGTGG AACGAAGTAA GCTCGGGTAC AGTTGATCTC 
ATGTCCAATC AATCCAAGCA GCAGTTTTCG CAAGGGAACT TAGCAGTGGT CCAGCAGAAG 
CAGAACTTTG GTCAGTACCA GAGCTACAAC CAGCAGCAGA TGCAGCTACC AGAGAACAGC 
ATGAATGTAA CACAGCAGAG CTTTATGCAA AGAAATGTGG GCATGGATGG GCAGAGGCTA 
AACTGTATGC AGCTGCGGCA GCAGCCAATG AGCCTAGGCC CAGGGATGAA CCCTGATCTG 
GGCTCGCAGG CAGGGTATAA CCAAGCTCAT CAGATGCTGA GCCCCAGTGC AATCAATGGC 
AACCCAAATC AGCTCTCTCC TTCTTGTAGC AACATGGTGA CAAAGTCTGG GCCCCACCTC 
CACCCTCAGC AAATGGACAT GGCGACCGAC CCATCCTTAA TGGTCAGCAG CAACAGCGAG 
CGCACAATGC TGAACCAGGT CATGCACGAA CCGAGCCAGC AGAACTACTC ATCTCAGCCA 
ACCCACATGA ACTTCCCCAT GGCTCAGGAG GCTTTCCATC AGCCCATCAT GCCCTCCAAC 
CAGCAGAGCT TTGAGCCCCA GCAGAGCATG ATGGGTTCGG CGGCGCAGAC GTATCCACCC 
GGCATGGTCC AACCTCGTCC CCCCGCTGAG CCCAGCCCGG CCAACAGGCC TCGAGGCCTC 
CGCACCGTGC AGCAGCTGGG CTACATGAGA ACTCCCCACC CTACAAACAC CATGAGCCCG 
GGCCAGGAGG CGGTGGAGGC TGTGCATAAA AGAACCGGCG ACGTGTTGCC AGCATCGACC 
CAACAGTGCG CGGATGGCAC GAGGGAAAAT AATTTGATGT ACTATTATGG TCAAATCCAC 
ATGTACGAAC AAAACAGCGG CTTTGATAAT CACTCGGACT GCAGGGTCAG GCAGCAGCAG 
TGCGCACTGA GCGGCAAGCC GGCCGCTCTG CCATCCCCGG GCACCAACCA GGTGTCCAGC 
ACGGTGGACT CACAAGGTCT TGAGCCGCCC CAGATAGATT TTGATGCCAT CATGGACGAT 
GGGGACCACT CGAGCCTGAT GTCAGGAACC CTGAGCCCCA GCATCCTGCA GAACCTCTCC 
CAGAACTCCT CTCGCCTGAC GACTCCACGA AACTCTCTGA CTCTGCCCAC CATACCCACG 
GGAATAAGCA ATATGGCAAT AGGAGATATG AGCTCCATGC TGACCACCCT GGCAGAAGAG 
AGTAAATTTC TCAACATGAT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145272.1
CDS	314..4900
Translation	METSASTVAGKKEGKGVVLEGDGFTETGKKPTPLAAAGAAVAQGVPQHIFPAFHAPLPIDMRHQEGRYHYEPHSIHAIHGPPPLSGSPVISDISLIRLSPHPAGPGESPFSPPHPYVTPHMEHYLRSVHGSPTLSVISAARGLSPADVAHEHLKERGLFGLPPPPPGANPTDYYHQMTLMAGHPNPYGDILMQSGGAAGTAHLHEYLSPVDVSRFSSPRVTPRLSRKRALSISPLSDASIDLQTMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGTISPAFSFPHPINPVTYQQILTQQRGLSSAFGHTPPLIQPSPTFPPRQHMAVISVNPPPAQISSNSNCISDSSQSKQSSESAVSSTVNPVINKRTKVKTEVEGLPPVSPTTQEHLTDLKEDLDKDECKQEPEVIYETNCHWEGCTKEYDTQEQLVHHINNDHIHGEKKEFVCRWQDCTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHPRPPPLKENGDNEASAKQSSKVSEESPEANSTTRSMEDCLQVKTIKTENSVMCQSSPGGQSSCSSEPSPLGSTNNNDSGVEMNMHSGGSLGDLTALDDNAPVVDSTVSSGNLTVSLQLRKHMTTMQRLEQLKKEKLKTVKDSCSWVNPAPQGRNTKLPPISGNGSILENSGGSSATLPNPRIMELSVNEVTMLNQINERRDSTTSTISSAYTVSRRSSGISPYFSSRRSSEASQLGHRPNNTSSADSYDPISTDASRRSSEASQCSGMPGLLNLTPAQHYRLKAKYAAATGGPPPTPLPNMERMTLKNRISLMDGPEPTLPSIRLPPGPRRCSDGNTYGYTSTAAFPHEVPGNCTRRASDPVRRPAGEPQALPRVHRFNSTNSMNPFHPPHPADRRNFSMQSYGRSDGSLARHTYSPRPLSISENVTMEAMSGEAEVPVGDDDIMLPDDVVQYIKSQNNGTAAESTSIGYSNEMQSFQGGGKLQPPAFPSQRRMAVADASMSHLGPMMAECPMSFDAPSDMNKNNMPVQWNEVSSGTVDLMSNQSKQQFSQGNLAVVQQKQNFGQYQSYNQQQMQLPENSMNVTQQSFMQRNVGMDGQRLNCMQLRQQPMSLGPGMNPDLGSQAGYNQAHQMLSPSAINGNPNQLSPSCSNMVTKSGPHLHPQQMDMATDPSLMVSSNSERTMLNQVMHEPSQQNYSSQPTHMNFPMAQEAFHQPIMPSNQQSFEPQQSMMGSAAQTYPPGMVQPRPPAEPSPANRPRGLRTVQQLGYMRTPHPTNTMSPGQEAVEAVHKRTGDVLPASTQQCADGTRENNLMYYYGQIHMYEQNSGFDNHSDCRVRQQQCALSGKPAALPSPGTNQVSSTVDSQGLEPPQIDFDAIMDDGDHSSLMSGTLSPSILQNLSQNSSRLTTPRNSLTLPTIPTGISNMAIGDMSSMLTTLAEESKFLNMMS

Target ORF information:

RefSeq Version	XM_015289786.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289786.2

ORF Insert Sequence:

ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA 
GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC 
ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG 
CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT 
CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC 
ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC 
AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG 
CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG 
GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG 
GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG 
ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT 
GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC 
AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT 
GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG 
AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT 
CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC 
AACAGCAACT GCATCTCCGA CTCCAGTCAG AGCAAGCAGA GCAGCGAGTC AGCCGTGAGC 
AGCACCGTCA ATCCCGTAAT TAACAAGCGC ACCAAAGTCA AGACCGAAGT CGAGGGCTTG 
CCCCCAGTAT CCCCAACCAC GCAGGAGCAT CTGACAGACC TGAAAGAAGA TCTAGATAAG 
GATGAATGTA AACAGGAGCC TGAGGTTATT TATGAGACTA ACTGTCACTG GGAAGGGTGC 
ACAAAGGAAT ATGACACTCA AGAGCAGCTC GTCCATCACA TCAACAATGA TCATATCCAT 
GGGGAGAAGA AGGAGTTTGT CTGCCGCTGG CAGGACTGCA CGCGGGAGCA GAAGCCCTTC 
AAAGCTCAAT ACATGCTGGT GGTGCACATG CGAAGGCACA CTGGAGAGAA GCCCCACAAG 
TGCACGTTTG AGGGTTGCTC CAAGGCTTAC TCCCGCCTGG AGAACTTAAA GACACACCTG 
AGGTCCCACA CTGGAGAAAA ACCTTACGTC TGTGAACACG AAGGCTGCAA TAAAGCCTTT 
TCCAATGCCT CGGATAGAGC CAAGCACCAG AACAGGACGC ATTCCAATGA GAAACCCTAT 
GTCTGTAAGA TCCCTGGCTG CACAAAGAGG TACACAGACC CTAGTTCCCT CAGGAAACAC 
GTCAAGACCG TACACGGACC TGATGCCCAC GTCACAAAGA AACAGCGCAA CGATGTCCAC 
CCAAGGCCGC CTCCGCTCAA GGAAAATGGG GACAACGAGG CGAGCGCCAA GCAGAGCAGT 
AAGGTGTCAG AGGAGAGCCC CGAGGCCAAC AGTACCACGA GGAGCATGGA GGATTGCTTA 
CAAGTCAAAA CGATAAAAAC AGAGAATTCT GTGATGTGTC AGTCCAGTCC TGGTGGCCAG 
TCATCATGCA GCAGTGAGCC GTCACCCCTG GGCAGCACCA ACAACAATGA CAGTGGAGTA 
GAAATGAACA TGCACAGCGG GGGAAGTCTG GGAGATCTGA CGGCGTTGGA TGACAACGCT 
CCTGTTGTGG ACTCAACGGT CTCATCTGGT AACTTGACAG TCAGCCTGCA GCTGAGGAAA 
CACATGACGA CAATGCAACG ACTTGAACAG CTCAAGAAAG AGAAACTCAA GACAGTTAAG 
GATTCCTGCT CATGGGTGAA CCCAGCTCCA CAAGGCAGAA ACACCAAGCT GCCTCCCATT 
TCAGGAAATG GCTCTATTCT AGAAAACAGT GGTGGTTCTT CTGCTACGCT GCCTAACCCA 
AGAATAATGG AGCTGTCTGT CAATGAGGTT ACGATGCTGA ACCAAATCAA TGAGCGCCGT 
GATAGTACGA CAAGCACCAT CAGCTCTGCC TACACCGTGA GCCGCAGATC ATCAGGGATT 
TCCCCATACT TCTCCAGCCG CCGCTCCAGC GAGGCTTCAC AGCTCGGGCA CCGTCCCAAT 
AACACGAGCT CTGCTGACTC TTATGATCCG ATTTCAACGG ATGCTTCCCG CCGGTCAAGT 
GAGGCGAGCC AGTGCAGCGG GATGCCGGGC CTACTGAACC TCACACCAGC TCAGCACTAC 
AGGCTGAAAG CCAAGTATGC TGCTGCCACA GGGGGCCCTC CTCCAACGCC CCTTCCAAAC 
ATGGAGAGGA TGACCCTGAA GAACAGAATC TCACTTATGG ATGGACCAGA GCCCACCTTG 
CCCTCCATTC GCCTCCCACC AGGCCCCAGG CGTTGCAGTG ATGGCAACAC CTATGGCTAC 
ACATCCACCG CAGCATTTCC CCATGAGGTT CCGGGCAACT GCACGAGACG GGCGAGCGAC 
CCTGTGAGGA GACCTGCTGG AGAGCCCCAG GCCCTCCCGA GAGTTCACCG CTTCAACAGC 
ACCAACAGCA TGAACCCCTT CCATCCTCCA CATCCTGCAG ACCGGAGGAA TTTCAGCATG 
CAGAGCTATG GGCGCTCTGA CGGGAGTCTT GCCCGGCACA CCTACTCACC CCGGCCACTG 
AGCATCAGTG AAAACGTCAC TATGGAAGCC ATGTCTGGGG AGGCAGAGGT GCCTGTTGGA 
GATGATGACA TTATGCTGCC AGATGATGTG GTGCAGTACA TCAAATCTCA GAACAACGGG 
ACAGCAGCTG AGAGCACTTC CATCGGGTAC AGCAATGAGA TGCAGAGCTT CCAGGGAGGC 
GGGAAGCTGC AGCCCCCAGC CTTTCCCAGC CAGCGCAGGA TGGCGGTGGC CGACGCGAGC 
ATGAGCCATT TGGGGCCCAT GATGGCAGAG TGTCCCATGA GCTTTGACGC GCCCTCAGAC 
ATGAATAAAA ATAACATGCC TGTCCAGTGG AACGAAGTAA GCTCGGGTAC AGTTGATCTC 
ATGTCCAATC AATCCAAGCA GCAGTTTTCG CAAGGGAACT TAGCAGTGGT CCAGCAGAAG 
CAGAACTTTG GTCAGTACCA GAGCTACAAC CAGCAGCAGA TGCAGCTACC AGAGAACAGC 
ATGAATGTAA CACAGCAGAG CTTTATGCAA AGAAATGTGG GCATGGATGG GCAGAGGCTA 
AACTGTATGC AGCTGCGGCA GCAGCCAATG AGCCTAGGCC CAGGGATGAA CCCTGATCTG 
GGCTCGCAGG CAGGGTATAA CCAAGCTCAT CAGATGCTGA GCCCCAGTGC AATCAATGGC 
AACCCAAATC AGCTCTCTCC TTCTTGTAGC AACATGGTGA CAAAGTCTGG GCCCCACCTC 
CACCCTCAGC AAATGGACAT GGCGACCGAC CCATCCTTAA TGGTCAGCAG CAACAGCGAG 
CGCACAATGC TGAACCAGGT CATGCACGAA CCGAGCCAGC AGAACTACTC ATCTCAGCCA 
ACCCACATGA ACTTCCCCAT GGCTCAGGAG GCTTTCCATC AGCCCATCAT GCCCTCCAAC 
CAGCAGAGCT TTGAGCCCCA GCAGAGCATG ATGGGTTCGG CGGCGCAGAC GTATCCACCC 
GGCATGGTCC AACCTCGTCC CCCCGCTGAG CCCAGCCCGG CCAACAGGCC TCGAGGCCTC 
CGCACCGTGC AGCAGCTGGG CTACATGAGA ACTCCCCACC CTACAAACAC CATGAGCCCG 
GGCCAGGAGG CGGTGGAGGC TGTGCATAAA AGAACCGGCG ACGTGTTGCC AGCATCGACC 
CAACAGTGCG CGGATGGCAC GAGGGAAAAT AATTTGATGT ACTATTATGG TCAAATCCAC 
ATGTACGAAC AAAACAGCGG CTTTGATAAT CACTCGGACT GCAGGGTCAG GCAGCAGCAG 
TGCGCACTGA GCGGCAAGCC GGCCGCTCTG CCATCCCCGG GCACCAACCA GGTGTCCAGC 
ACGGTGGACT CACAAGGTCT TGAGCCGCCC CAGATAGATT TTGATGCCAT CATGGACGAT 
GGGGACCACT CGAGCCTGAT GTCAGGAACC CTGAGCCCCA GCATCCTGCA GAACCTCTCC 
CAGAACTCCT CTCGCCTGAC GACTCCACGA AACTCTCTGA CTCTGCCCAC CATACCCACG 
GGAATAAGCA ATATGGCAAT AGGAGATATG AGCTCCATGC TGACCACCCT GGCAGAAGAG 
AGTAAATTTC TCAACATGAT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A potential role of alternative splicing in the regulation of the transcriptional activity of human GLI2 in gonadal tissues.
BMC molecular biology713(2006 Mar)
Speek M,Njunkova O,Pata I,Valdre E,Kogerman P

Similar expression and regulation of Gli2 and Gli3 in the chick limb bud.
Mechanisms of development98(1-2)171-4(2000 Nov)
Schweitzer R,Vogan KJ,Tabin CJ

Sonic hedgehog differentially regulates expression of GLI and GLI3 during limb development.
Developmental biology180(1)273-83(1996 Nov)
Marigo V,Johnson RL,Vortkamp A,Tabin CJ

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GLI2 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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