Ap2a2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ap2a2
Entrez Gene ID	11772
Full Name	adaptor-related protein complex 2, alpha 2 subunit
Synonyms	2410074K14Rik,AF006990,AW146353,Adtab,C78001,L25
General protein information	Preferred Names adaptor-related protein complex 2, alpha 2 subunit Names AP-2 complex subunit alpha-2 100 kDa coated vesicle protein C adapter-related protein complex 2 alpha-2 subunit adapter-related protein complex 2 subunit alpha-2 adaptor protein complex AP-2 subunit alpha-2 adaptor protein complex AP-2, alpha 2 subunit adaptor-related protein complex 2 subunit alpha-2 adaptor-related protein complex AP-2, alpha 2 subunit alpha-C adaptin alpha-adaptin C alpha2-adaptin clathrin assembly protein complex 2 alpha-C large chain plasma membrane adaptor HA2/AP2 adaptin alpha C subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F5

mRNA and Protein(s)

mRNA	Protein	Name
XM_006536154.1	XP_006536217.1	AP-2 complex subunit alpha-2 isoform X1
XM_006536155.4	XP_006536218.1	AP-2 complex subunit alpha-2 isoform X1
XM_006536155.3	XP_006536218.1	AP-2 complex subunit alpha-2 isoform X2
NM_001357068.1	NP_001343997.1	AP-2 complex subunit alpha-2 isoform 1
NM_007459.3	NP_031485.3	AP-2 complex subunit alpha-2 isoform 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	AP2A2	NP_001012914.1
Drosophila melanogaster (fruit fly)	alpha-Adaptin	NP_476819.2
Caenorhabditis elegans (roundworm)	apa-2	NP_509572.1
Neurospora crassa	NCU03440	XP_956726.1
Arabidopsis thaliana (thale cress)	alpha-ADR	NP_197669.1
Pan troglodytes (chimpanzee)	AP2A2	XP_003951843.1
Rattus norvegicus (Norway rat)	Ap2a2	NP_112270.2
Anopheles gambiae (African malaria mosquito)	AP2A_ANOGA	XP_310153.4
Arabidopsis thaliana (thale cress)	AT5G22780	NP_197670.1
Homo sapiens (human)	AP2A2	NP_001229766.1
	AP2A2	XP_001116794.2
Schizosaccharomyces pombe (fission yeast)	apl3	NP_595595.1
Magnaporthe oryzae (rice blast fungus)	MGG_09493	XP_003712251.1
Canis lupus familiaris (dog)	AP2A2	XP_005631788.1
Xenopus tropicalis (tropical clawed frog)	ap2a2	NP_001001209.1
Bos taurus (cattle)	AP2A2	NP_001069170.1
Mus musculus (house mouse)	Ap2a2	NP_031485.3

Gene Ontology
Bibliography

Related articles in PubMed

APache Is an AP2-Interacting Protein Involved in Synaptic Vesicle Trafficking and Neuronal Development.
Piccini A, Castroflorio E, Valente P, Guarnieri FC, Aprile D, Michetti C, Bramini M, Giansante G, Pinto B, Savardi A, Cesca F, Bachi A, Cattaneo A, Wren JD, Fassio A, Valtorta F, Benfenati F, Gioved? S
Cell reports21(12)3596-3611(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that dynamin, clathrin, adaptor protein complex-2 (AP2), and amphiphysin contribute to the depolarization-evoked entry in endocytosis.
Title: Molecular components required for resting and stimulated endocytosis of botulinum neurotoxins by glutamatergic and peptidergic neurons.

PPARalpha regulates the mouse cardiac AP2alpha2 gene in both the control and transgenic mouse.
Title: The adaptor-related protein complex 2, alpha 2 subunit (AP2?2) gene is a peroxisome proliferator-activated receptor cardiac target gene.

NMR structure of mouse alpha-adaptin ear-domain
Title: 1H, 15N and 13C resonance assignments and 15N-1H residual dipolar couplings for the alpha-adaptin ear-domain.

the AP-2 complex is not associated with the endocytosed plasma membrane-associated clathrin
Title: The AP-2 complex is excluded from the dynamic population of plasma membrane-associated clathrin.

Tensile stress induces osteoblast differentiation possibly through the induction of alpha-adaptin C gene expression.
Title: Tensile stress induces alpha-adaptin C production in mouse calvariae in an organ culture: possible involvement of endocytosis in mechanical stress-stimulated osteoblast differentiation.

The following Ap2a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ap2a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu40039	NM_001357068.1 Latest version!	Mus musculus adaptor-related protein complex 2, alpha 2 subunit (Ap2a2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu40039	XM_006536154.1 Latest version!	Mus musculus adaptor-related protein complex 2, alpha 2 subunit (Ap2a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu19986	NM_007459.3 Latest version!	Mus musculus adaptor-related protein complex 2, alpha 2 subunit (Ap2a2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$552.30 $789.00
OMu40040	XM_006536155.4 Latest version!	Mus musculus adaptor-related protein complex 2, alpha 2 subunit (Ap2a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu40040	XM_006536155.3	Mus musculus adaptor-related protein complex 2, alpha 2 subunit (Ap2a2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ap2a2 ( XM_006536155.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536218.1 Mus musculus adaptor-related protein complex 2, alpha 2 subunit (Ap2a2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu40040
Clone ID Related Accession (Same CDS sequence)	XM_006536155.4 , XM_006536155.3
Accession Version	XM_006536155.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	AP-2 complex subunit alpha-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006536155.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGACC GGAGCAACGC CCAGCAGATT GTGGCTGAGA TGCTGAGCTA CCTAGAAACG 
GCTGACTACT CCATCCGAGA GGAAATTGTG CTGAAGGTGG CCATCCTGGC TGAGAAGTAT 
GCAGTGGACT ACACTTGGTA TGTGGACACC ATCCTCAACC TCATCCGCAT CGCGGGCGAC 
TATGTGAGCG AGGAGGTGTG GTACCGCGTC ATCCAGATTG TCATCAATCG CGATGATGTG 
CAGGGCTATG CTGCCAAGAC GGTATTTGAG GCATTGCAGG CGCCAGCATG TCATGAGAAC 
TTGGTCAAAG TGGGCGGCTA CATCCTGGGG GAGTTTGGAA ACTTGATAGC TGGGGACCCA 
CGATCCAGCC CTCTGATCCA GTTCAACCTG CTCCACTCCA AGTTCCACTT GTGCAGCGTC 
CCCACTCGGG CACTCCTGCT GTCCACCTAC ATCAAGTTCG TGAACCTCTT TCCAGAGGTA 
AAAGCTACCA TTCAGGACGT GCTGCGCAGT GACAGCCAGC TGAAGAATGC CGACGTGGAG 
CTGCAGCAGC GGGCTGTGGA GTACCTGCGA CTGAGCACTG TCGCCAGCAC TGACATCCTG 
GCAACTGTCC TGGAGGAAAT GCCACCCTTC CCTGAGCGTG AGTCCTCCAT CTTGGCCAAG 
CTGAAGAAGA AGAAGGGCCC AAGCACAGTG ACTGACCTGG AGGAGACCAA GCGGGAGCGA 
AGCATCGATG TGAATGGGGG CCCTGAACCC GTTCCAGCCA GCACCAGTGC TGCGTCCACA 
CCTTCTCCGT CAGCAGACCT GCTGGGTCTG GGGGCCGTGC CCCCTGCTCC CACGGGGCCC 
CCTCCCTCCT CTGGCGGCGG GCTGCTGGTG GACGTGTTCT CAGACTCAGC TTCTGCAGTC 
GCACCTCTTG CACCCGGCTC TGAAGACAAC TTTGCCAGGT TTGTTTGTAA AAATAATGGT 
GTATTGTTCG AAAACCAGCT GCTTCAAATT GGACTTAAGT CAGAATTTCG GCAGAATTTA 
GGCCGAATGT TCATATTTTA TGGTAACAAG ACCTCCACGC AGTTCCTAAA CTTCACTCCA 
ACACTAATCT GTGCTGACGA CCTTCAAACT AATCTGAACC TGCAGACTAA GCCCGTGGAC 
CCAACTGTGG ATGGGGGCGC ACAGGTGCAA CAAGTGGTCA ACATTGAGTG TATATCTGAT 
TTCACAGAGG CACCTGTCCT CAACATCCAG TTCAGGTATG GTGGGACCTT CCAGAATGTG 
TCTGTTAAGC TTCCCATCAC TCTCAACAAA TTTTTCCAGC CCACAGAAAT GGCTTCTCAG 
GATTTCTTTC AACGTTGGAA GCAGTTGAGC AATCCACAGC AAGAAGTGCA GAATATCTTC 
AAAGCAAAGC ATCCGATGGA CACAGAGATC ACTAAGGCAA AGATTATTGG ATTTGGTTCT 
GCGCTCCTGG AAGAAGTTGA CCCGAATCCT GCAAATTTCG TGGGTGCTGG CATAATACAC 
ACCAAAACCA CCCAGATTGG CTGCTTGTTG CGCCTGGAGC CAAACCTGCA AGCTCAGATG 
TATAGACTCA CCTTGCGTAC CAGCAAAGAC ACCGTCTCTC AGAGACTATG TGAATTGCTG 
TCGGAACAGT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536218.1
CDS	778..2412
Translation	MCDRSNAQQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFNLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKATIQDVLRSDSQLKNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEETKRERSIDVNGGPEPVPASTSAASTPSPSADLLGLGAVPPAPTGPPPSSGGGLLVDVFSDSASAVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICADDLQTNLNLQTKPVDPTVDGGAQVQQVVNIECISDFTEAPVLNIQFRYGGTFQNVSVKLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEITKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKDTVSQRLCELLSEQF

Target ORF information:

RefSeq Version	XM_006536155.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor-related protein complex 2, alpha 2 subunit (Ap2a2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536155.3

ORF Insert Sequence:

ATGTGTGACC GGAGCAACGC CCAGCAGATT GTGGCTGAGA TGCTGAGCTA CCTAGAAACG 
GCTGACTACT CCATCCGAGA GGAAATTGTG CTGAAGGTGG CCATCCTGGC TGAGAAGTAT 
GCAGTGGACT ACACTTGGTA TGTGGACACC ATCCTCAACC TCATCCGCAT CGCGGGCGAC 
TATGTGAGCG AGGAGGTGTG GTACCGCGTC ATCCAGATTG TCATCAATCG CGATGATGTG 
CAGGGCTATG CTGCCAAGAC GGTATTTGAG GCATTGCAGG CGCCAGCATG TCATGAGAAC 
TTGGTCAAAG TGGGCGGCTA CATCCTGGGG GAGTTTGGAA ACTTGATAGC TGGGGACCCA 
CGATCCAGCC CTCTGATCCA GTTCAACCTG CTCCACTCCA AGTTCCACTT GTGCAGCGTC 
CCCACTCGGG CACTCCTGCT GTCCACCTAC ATCAAGTTCG TGAACCTCTT TCCAGAGGTA 
AAAGCTACCA TTCAGGACGT GCTGCGCAGT GACAGCCAGC TGAAGAATGC CGACGTGGAG 
CTGCAGCAGC GGGCTGTGGA GTACCTGCGA CTGAGCACTG TCGCCAGCAC TGACATCCTG 
GCAACTGTCC TGGAGGAAAT GCCACCCTTC CCTGAGCGTG AGTCCTCCAT CTTGGCCAAG 
CTGAAGAAGA AGAAGGGCCC AAGCACAGTG ACTGACCTGG AGGAGACCAA GCGGGAGCGA 
AGCATCGATG TGAATGGGGG CCCTGAACCC GTTCCAGCCA GCACCAGTGC TGCGTCCACA 
CCTTCTCCGT CAGCAGACCT GCTGGGTCTG GGGGCCGTGC CCCCTGCTCC CACGGGGCCC 
CCTCCCTCCT CTGGCGGCGG GCTGCTGGTG GACGTGTTCT CAGACTCAGC TTCTGCAGTC 
GCACCTCTTG CACCCGGCTC TGAAGACAAC TTTGCCAGGT TTGTTTGTAA AAATAATGGT 
GTATTGTTCG AAAACCAGCT GCTTCAAATT GGACTTAAGT CAGAATTTCG GCAGAATTTA 
GGCCGAATGT TCATATTTTA TGGTAACAAG ACCTCCACGC AGTTCCTAAA CTTCACTCCA 
ACACTAATCT GTGCTGACGA CCTTCAAACT AATCTGAACC TGCAGACTAA GCCCGTGGAC 
CCAACTGTGG ATGGGGGCGC ACAGGTGCAA CAAGTGGTCA ACATTGAGTG TATATCTGAT 
TTCACAGAGG CACCTGTCCT CAACATCCAG TTCAGGTATG GTGGGACCTT CCAGAATGTG 
TCTGTTAAGC TTCCCATCAC TCTCAACAAA TTTTTCCAGC CCACAGAAAT GGCTTCTCAG 
GATTTCTTTC AACGTTGGAA GCAGTTGAGC AATCCACAGC AAGAAGTGCA GAATATCTTC 
AAAGCAAAGC ATCCGATGGA CACAGAGATC ACTAAGGCAA AGATTATTGG ATTTGGTTCT 
GCGCTCCTGG AAGAAGTTGA CCCGAATCCT GCAAATTTCG TGGGTGCTGG CATAATACAC 
ACCAAAACCA CCCAGATTGG CTGCTTGTTG CGCCTGGAGC CAAACCTGCA AGCTCAGATG 
TATAGACTCA CCTTGCGTAC CAGCAAAGAC ACCGTCTCTC AGAGACTATG TGAATTGCTG 
TCGGAACAGT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap2a2 ( NM_001357068.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001343997.1 Mus musculus adaptor-related protein complex 2, alpha 2 subunit (Ap2a2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu40039
Clone ID Related Accession (Same CDS sequence)	XM_006536154.1 , NM_001357068.1
Accession Version	NM_001357068.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	AP-2 complex subunit alpha-2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158224.6 and AC102524.11. On Oct 18, 2017 this sequence version replaced XM_006536154.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.54131.1, SRR1660811.218651.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGCCG TATCCAAGGG GGACGGGATG CGAGGCCTAG CGGTCTTCAT CTCCGACATC 
CGCAACTGTA AAAGTAAAGA AGCTGAAATA AAGAGAATAA ACAAAGAACT GGCAAATATT 
AGATCCAAAT TTAAAGGTGA CAAGGCTCTT GATGGCTACA GTAAAAAGAA GTATGTCTGC 
AAATTGCTCT TCATCTTTCT CCTTGGTCAT GACATTGACT TTGGACACAT GGAAGCTGTG 
AATCTTCTGA GCTCAAACAG ATACACGGAA AAGCAGATTG GCTACCTTTT CATCTCTGTA 
TTGGTGAACT CGAATAGTGA ACTGATCCGG TTGATTAACA ATGCCATCAA GAATGACCTG 
GCCAGCCGCA ATCCTACGTT TATGGGTCTC GCCCTGCACT GCATTGCCAA TGTGGGTAGC 
CGTGAGATGG CGGAGGCCTT CGCCGGAGAG ATCCCCAAGA TCCTCGTGGC AGGAGATACC 
ATGGATAGTG TGAAGCAGAG CGCAGCCCTG TGCTTGTTAC GCCTGTACAG GACATCACCT 
GACCTAGTTC CCATGGGCGA CTGGACATCT CGTGTGGTAC ACCTCCTCAA TGACCAGCAC 
TTGGGGGTAG TGACTGCAGC GACTAGCCTC ATCACCACCC TGGCCCAGAA GAACCCTGAG 
GAGTTCAAGA CCTCTGTCTC GCTAGCTGTC TCGCGACTGA GCAGGATTGT GACGTCTGCA 
TCAACAGATC TTCAGGATTA TACTTACTAC TTTGTCCCGG CTCCTTGGCT GTCAGTCAAG 
CTTCTGAGAC TGCTGCAGTG CTACCCACCC CCAGAAGACC CTGCGGTGCG TGGCCGCCTG 
ACTGAGTGCT TGGAGACCAT CCTGAACAAG GCCCAGGAGC CACCCAAGTC CAAGAAGGTC 
CAGCATTCAA ATGCCAAGAA CGCTGTGCTG TTTGAGGCCA TCAGCCTGAT CATCCACCAT 
GACAGTGAGC CAAACTTGCT CGTCCGCGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACGA ACCTACGCTA CCTGGCTCTG GAGAGCATGT GCACGCTGGC CAGCTCTGAG 
TTCTCCCACG AGGCCGTCAA GACCCACATT GAGACGGTCA TCAATGCCTT GAAGACGGAA 
AGAGACGTGA GTGTGAGGCA GCGGGCAGTG GACCTGCTCT ACGCCATGTG TGACCGGAGC 
AACGCCCAGC AGATTGTGGC TGAGATGCTG AGCTACCTAG AAACGGCTGA CTACTCCATC 
CGAGAGGAAA TTGTGCTGAA GGTGGCCATC CTGGCTGAGA AGTATGCAGT GGACTACACT 
TGGTATGTGG ACACCATCCT CAACCTCATC CGCATCGCGG GCGACTATGT GAGCGAGGAG 
GTGTGGTACC GCGTCATCCA GATTGTCATC AATCGCGATG ATGTGCAGGG CTATGCTGCC 
AAGACGGTAT TTGAGGCATT GCAGGCGCCA GCATGTCATG AGAACTTGGT CAAAGTGGGC 
GGCTACATCC TGGGGGAGTT TGGAAACTTG ATAGCTGGGG ACCCACGATC CAGCCCTCTG 
ATCCAGTTCA ACCTGCTCCA CTCCAAGTTC CACTTGTGCA GCGTCCCCAC TCGGGCACTC 
CTGCTGTCCA CCTACATCAA GTTCGTGAAC CTCTTTCCAG AGGTAAAAGC TACCATTCAG 
GACGTGCTGC GCAGTGACAG CCAGCTGAAG AATGCCGACG TGGAGCTGCA GCAGCGGGCT 
GTGGAGTACC TGCGACTGAG CACTGTCGCC AGCACTGACA TCCTGGCAAC TGTCCTGGAG 
GAAATGCCAC CCTTCCCTGA GCGTGAGTCC TCCATCTTGG CCAAGCTGAA GAAGAAGAAG 
GGCCCAAGCA CAGTGACTGA CCTGGAGGAG ACCAAGCGGG AGCGAAGCAT CGATGTGAAT 
GGGGGCCCTG AACCCGTTCC AGCCAGCACC AGTGCTGCGT CCACACCTTC TCCGTCAGCA 
GACCTGCTGG GTCTGGGGGC CGTGCCCCCT GCTCCCACGG GGCCCCCTCC CTCCTCTGGC 
GGCGGGCTGC TGGTGGACGT GTTCTCAGAC TCAGCTTCTG CAGTCGCACC TCTTGCACCC 
GGCTCTGAAG ACAACTTTGC CAGGTTTGTT TGTAAAAATA ATGGTGTATT GTTCGAAAAC 
CAGCTGCTTC AAATTGGACT TAAGTCAGAA TTTCGGCAGA ATTTAGGCCG AATGTTCATA 
TTTTATGGTA ACAAGACCTC CACGCAGTTC CTAAACTTCA CTCCAACACT AATCTGTGCT 
GACGACCTTC AAACTAATCT GAACCTGCAG ACTAAGCCCG TGGACCCAAC TGTGGATGGG 
GGCGCACAGG TGCAACAAGT GGTCAACATT GAGTGTATAT CTGATTTCAC AGAGGCACCT 
GTCCTCAACA TCCAGTTCAG GTATGGTGGG ACCTTCCAGA ATGTGTCTGT TAAGCTTCCC 
ATCACTCTCA ACAAATTTTT CCAGCCCACA GAAATGGCTT CTCAGGATTT CTTTCAACGT 
TGGAAGCAGT TGAGCAATCC ACAGCAAGAA GTGCAGAATA TCTTCAAAGC AAAGCATCCG 
ATGGACACAG AGATCACTAA GGCAAAGATT ATTGGATTTG GTTCTGCGCT CCTGGAAGAA 
GTTGACCCGA ATCCTGCAAA TTTCGTGGGT GCTGGCATAA TACACACCAA AACCACCCAG 
ATTGGCTGCT TGTTGCGCCT GGAGCCAAAC CTGCAAGCTC AGATGTATAG ACTCACCTTG 
CGTACCAGCA AAGACACCGT CTCTCAGAGA CTATGTGAAT TGCTGTCGGA ACAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343997.1
CDS	181..3000
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIANVGSREMAEAFAGEIPKILVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDPAVRGRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAQQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFNLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKATIQDVLRSDSQLKNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEETKRERSIDVNGGPEPVPASTSAASTPSPSADLLGLGAVPPAPTGPPPSSGGGLLVDVFSDSASAVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICADDLQTNLNLQTKPVDPTVDGGAQVQQVVNIECISDFTEAPVLNIQFRYGGTFQNVSVKLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEITKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKDTVSQRLCELLSEQF

Target ORF information:

RefSeq Version	NM_001357068.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor-related protein complex 2, alpha 2 subunit (Ap2a2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357068.1

ORF Insert Sequence:

ATGCCGGCCG TATCCAAGGG GGACGGGATG CGAGGCCTAG CGGTCTTCAT CTCCGACATC 
CGCAACTGTA AAAGTAAAGA AGCTGAAATA AAGAGAATAA ACAAAGAACT GGCAAATATT 
AGATCCAAAT TTAAAGGTGA CAAGGCTCTT GATGGCTACA GTAAAAAGAA GTATGTCTGC 
AAATTGCTCT TCATCTTTCT CCTTGGTCAT GACATTGACT TTGGACACAT GGAAGCTGTG 
AATCTTCTGA GCTCAAACAG ATACACGGAA AAGCAGATTG GCTACCTTTT CATCTCTGTA 
TTGGTGAACT CGAATAGTGA ACTGATCCGG TTGATTAACA ATGCCATCAA GAATGACCTG 
GCCAGCCGCA ATCCTACGTT TATGGGTCTC GCCCTGCACT GCATTGCCAA TGTGGGTAGC 
CGTGAGATGG CGGAGGCCTT CGCCGGAGAG ATCCCCAAGA TCCTCGTGGC AGGAGATACC 
ATGGATAGTG TGAAGCAGAG CGCAGCCCTG TGCTTGTTAC GCCTGTACAG GACATCACCT 
GACCTAGTTC CCATGGGCGA CTGGACATCT CGTGTGGTAC ACCTCCTCAA TGACCAGCAC 
TTGGGGGTAG TGACTGCAGC GACTAGCCTC ATCACCACCC TGGCCCAGAA GAACCCTGAG 
GAGTTCAAGA CCTCTGTCTC GCTAGCTGTC TCGCGACTGA GCAGGATTGT GACGTCTGCA 
TCAACAGATC TTCAGGATTA TACTTACTAC TTTGTCCCGG CTCCTTGGCT GTCAGTCAAG 
CTTCTGAGAC TGCTGCAGTG CTACCCACCC CCAGAAGACC CTGCGGTGCG TGGCCGCCTG 
ACTGAGTGCT TGGAGACCAT CCTGAACAAG GCCCAGGAGC CACCCAAGTC CAAGAAGGTC 
CAGCATTCAA ATGCCAAGAA CGCTGTGCTG TTTGAGGCCA TCAGCCTGAT CATCCACCAT 
GACAGTGAGC CAAACTTGCT CGTCCGCGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACGA ACCTACGCTA CCTGGCTCTG GAGAGCATGT GCACGCTGGC CAGCTCTGAG 
TTCTCCCACG AGGCCGTCAA GACCCACATT GAGACGGTCA TCAATGCCTT GAAGACGGAA 
AGAGACGTGA GTGTGAGGCA GCGGGCAGTG GACCTGCTCT ACGCCATGTG TGACCGGAGC 
AACGCCCAGC AGATTGTGGC TGAGATGCTG AGCTACCTAG AAACGGCTGA CTACTCCATC 
CGAGAGGAAA TTGTGCTGAA GGTGGCCATC CTGGCTGAGA AGTATGCAGT GGACTACACT 
TGGTATGTGG ACACCATCCT CAACCTCATC CGCATCGCGG GCGACTATGT GAGCGAGGAG 
GTGTGGTACC GCGTCATCCA GATTGTCATC AATCGCGATG ATGTGCAGGG CTATGCTGCC 
AAGACGGTAT TTGAGGCATT GCAGGCGCCA GCATGTCATG AGAACTTGGT CAAAGTGGGC 
GGCTACATCC TGGGGGAGTT TGGAAACTTG ATAGCTGGGG ACCCACGATC CAGCCCTCTG 
ATCCAGTTCA ACCTGCTCCA CTCCAAGTTC CACTTGTGCA GCGTCCCCAC TCGGGCACTC 
CTGCTGTCCA CCTACATCAA GTTCGTGAAC CTCTTTCCAG AGGTAAAAGC TACCATTCAG 
GACGTGCTGC GCAGTGACAG CCAGCTGAAG AATGCCGACG TGGAGCTGCA GCAGCGGGCT 
GTGGAGTACC TGCGACTGAG CACTGTCGCC AGCACTGACA TCCTGGCAAC TGTCCTGGAG 
GAAATGCCAC CCTTCCCTGA GCGTGAGTCC TCCATCTTGG CCAAGCTGAA GAAGAAGAAG 
GGCCCAAGCA CAGTGACTGA CCTGGAGGAG ACCAAGCGGG AGCGAAGCAT CGATGTGAAT 
GGGGGCCCTG AACCCGTTCC AGCCAGCACC AGTGCTGCGT CCACACCTTC TCCGTCAGCA 
GACCTGCTGG GTCTGGGGGC CGTGCCCCCT GCTCCCACGG GGCCCCCTCC CTCCTCTGGC 
GGCGGGCTGC TGGTGGACGT GTTCTCAGAC TCAGCTTCTG CAGTCGCACC TCTTGCACCC 
GGCTCTGAAG ACAACTTTGC CAGGTTTGTT TGTAAAAATA ATGGTGTATT GTTCGAAAAC 
CAGCTGCTTC AAATTGGACT TAAGTCAGAA TTTCGGCAGA ATTTAGGCCG AATGTTCATA 
TTTTATGGTA ACAAGACCTC CACGCAGTTC CTAAACTTCA CTCCAACACT AATCTGTGCT 
GACGACCTTC AAACTAATCT GAACCTGCAG ACTAAGCCCG TGGACCCAAC TGTGGATGGG 
GGCGCACAGG TGCAACAAGT GGTCAACATT GAGTGTATAT CTGATTTCAC AGAGGCACCT 
GTCCTCAACA TCCAGTTCAG GTATGGTGGG ACCTTCCAGA ATGTGTCTGT TAAGCTTCCC 
ATCACTCTCA ACAAATTTTT CCAGCCCACA GAAATGGCTT CTCAGGATTT CTTTCAACGT 
TGGAAGCAGT TGAGCAATCC ACAGCAAGAA GTGCAGAATA TCTTCAAAGC AAAGCATCCG 
ATGGACACAG AGATCACTAA GGCAAAGATT ATTGGATTTG GTTCTGCGCT CCTGGAAGAA 
GTTGACCCGA ATCCTGCAAA TTTCGTGGGT GCTGGCATAA TACACACCAA AACCACCCAG 
ATTGGCTGCT TGTTGCGCCT GGAGCCAAAC CTGCAAGCTC AGATGTATAG ACTCACCTTG 
CGTACCAGCA AAGACACCGT CTCTCAGAGA CTATGTGAAT TGCTGTCGGA ACAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap2a2 ( XM_006536154.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536217.1 Mus musculus adaptor-related protein complex 2, alpha 2 subunit (Ap2a2), transcript variant X1, mRNA.

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CloneID	OMu40039
Clone ID Related Accession (Same CDS sequence)	XM_006536154.1 , NM_001357068.1
Accession Version	XM_006536154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	AP-2 complex subunit alpha-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGCCG TATCCAAGGG GGACGGGATG CGAGGCCTAG CGGTCTTCAT CTCCGACATC 
CGCAACTGTA AAAGTAAAGA AGCTGAAATA AAGAGAATAA ACAAAGAACT GGCAAATATT 
AGATCCAAAT TTAAAGGTGA CAAGGCTCTT GATGGCTACA GTAAAAAGAA GTATGTCTGC 
AAATTGCTCT TCATCTTTCT CCTTGGTCAT GACATTGACT TTGGACACAT GGAAGCTGTG 
AATCTTCTGA GCTCAAACAG ATACACGGAA AAGCAGATTG GCTACCTTTT CATCTCTGTA 
TTGGTGAACT CGAATAGTGA ACTGATCCGG TTGATTAACA ATGCCATCAA GAATGACCTG 
GCCAGCCGCA ATCCTACGTT TATGGGTCTC GCCCTGCACT GCATTGCCAA TGTGGGTAGC 
CGTGAGATGG CGGAGGCCTT CGCCGGAGAG ATCCCCAAGA TCCTCGTGGC AGGAGATACC 
ATGGATAGTG TGAAGCAGAG CGCAGCCCTG TGCTTGTTAC GCCTGTACAG GACATCACCT 
GACCTAGTTC CCATGGGCGA CTGGACATCT CGTGTGGTAC ACCTCCTCAA TGACCAGCAC 
TTGGGGGTAG TGACTGCAGC GACTAGCCTC ATCACCACCC TGGCCCAGAA GAACCCTGAG 
GAGTTCAAGA CCTCTGTCTC GCTAGCTGTC TCGCGACTGA GCAGGATTGT GACGTCTGCA 
TCAACAGATC TTCAGGATTA TACTTACTAC TTTGTCCCGG CTCCTTGGCT GTCAGTCAAG 
CTTCTGAGAC TGCTGCAGTG CTACCCACCC CCAGAAGACC CTGCGGTGCG TGGCCGCCTG 
ACTGAGTGCT TGGAGACCAT CCTGAACAAG GCCCAGGAGC CACCCAAGTC CAAGAAGGTC 
CAGCATTCAA ATGCCAAGAA CGCTGTGCTG TTTGAGGCCA TCAGCCTGAT CATCCACCAT 
GACAGTGAGC CAAACTTGCT CGTCCGCGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACGA ACCTACGCTA CCTGGCTCTG GAGAGCATGT GCACGCTGGC CAGCTCTGAG 
TTCTCCCACG AGGCCGTCAA GACCCACATT GAGACGGTCA TCAATGCCTT GAAGACGGAA 
AGAGACGTGA GTGTGAGGCA GCGGGCAGTG GACCTGCTCT ACGCCATGTG TGACCGGAGC 
AACGCCCAGC AGATTGTGGC TGAGATGCTG AGCTACCTAG AAACGGCTGA CTACTCCATC 
CGAGAGGAAA TTGTGCTGAA GGTGGCCATC CTGGCTGAGA AGTATGCAGT GGACTACACT 
TGGTATGTGG ACACCATCCT CAACCTCATC CGCATCGCGG GCGACTATGT GAGCGAGGAG 
GTGTGGTACC GCGTCATCCA GATTGTCATC AATCGCGATG ATGTGCAGGG CTATGCTGCC 
AAGACGGTAT TTGAGGCATT GCAGGCGCCA GCATGTCATG AGAACTTGGT CAAAGTGGGC 
GGCTACATCC TGGGGGAGTT TGGAAACTTG ATAGCTGGGG ACCCACGATC CAGCCCTCTG 
ATCCAGTTCA ACCTGCTCCA CTCCAAGTTC CACTTGTGCA GCGTCCCCAC TCGGGCACTC 
CTGCTGTCCA CCTACATCAA GTTCGTGAAC CTCTTTCCAG AGGTAAAAGC TACCATTCAG 
GACGTGCTGC GCAGTGACAG CCAGCTGAAG AATGCCGACG TGGAGCTGCA GCAGCGGGCT 
GTGGAGTACC TGCGACTGAG CACTGTCGCC AGCACTGACA TCCTGGCAAC TGTCCTGGAG 
GAAATGCCAC CCTTCCCTGA GCGTGAGTCC TCCATCTTGG CCAAGCTGAA GAAGAAGAAG 
GGCCCAAGCA CAGTGACTGA CCTGGAGGAG ACCAAGCGGG AGCGAAGCAT CGATGTGAAT 
GGGGGCCCTG AACCCGTTCC AGCCAGCACC AGTGCTGCGT CCACACCTTC TCCGTCAGCA 
GACCTGCTGG GTCTGGGGGC CGTGCCCCCT GCTCCCACGG GGCCCCCTCC CTCCTCTGGC 
GGCGGGCTGC TGGTGGACGT GTTCTCAGAC TCAGCTTCTG CAGTCGCACC TCTTGCACCC 
GGCTCTGAAG ACAACTTTGC CAGGTTTGTT TGTAAAAATA ATGGTGTATT GTTCGAAAAC 
CAGCTGCTTC AAATTGGACT TAAGTCAGAA TTTCGGCAGA ATTTAGGCCG AATGTTCATA 
TTTTATGGTA ACAAGACCTC CACGCAGTTC CTAAACTTCA CTCCAACACT AATCTGTGCT 
GACGACCTTC AAACTAATCT GAACCTGCAG ACTAAGCCCG TGGACCCAAC TGTGGATGGG 
GGCGCACAGG TGCAACAAGT GGTCAACATT GAGTGTATAT CTGATTTCAC AGAGGCACCT 
GTCCTCAACA TCCAGTTCAG GTATGGTGGG ACCTTCCAGA ATGTGTCTGT TAAGCTTCCC 
ATCACTCTCA ACAAATTTTT CCAGCCCACA GAAATGGCTT CTCAGGATTT CTTTCAACGT 
TGGAAGCAGT TGAGCAATCC ACAGCAAGAA GTGCAGAATA TCTTCAAAGC AAAGCATCCG 
ATGGACACAG AGATCACTAA GGCAAAGATT ATTGGATTTG GTTCTGCGCT CCTGGAAGAA 
GTTGACCCGA ATCCTGCAAA TTTCGTGGGT GCTGGCATAA TACACACCAA AACCACCCAG 
ATTGGCTGCT TGTTGCGCCT GGAGCCAAAC CTGCAAGCTC AGATGTATAG ACTCACCTTG 
CGTACCAGCA AAGACACCGT CTCTCAGAGA CTATGTGAAT TGCTGTCGGA ACAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536217.1
CDS	176..2995
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIANVGSREMAEAFAGEIPKILVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDPAVRGRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAQQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFNLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKATIQDVLRSDSQLKNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEETKRERSIDVNGGPEPVPASTSAASTPSPSADLLGLGAVPPAPTGPPPSSGGGLLVDVFSDSASAVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICADDLQTNLNLQTKPVDPTVDGGAQVQQVVNIECISDFTEAPVLNIQFRYGGTFQNVSVKLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEITKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKDTVSQRLCELLSEQF

Target ORF information:

RefSeq Version	XM_006536154.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus adaptor-related protein complex 2, alpha 2 subunit (Ap2a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536154.1

ORF Insert Sequence:

ATGCCGGCCG TATCCAAGGG GGACGGGATG CGAGGCCTAG CGGTCTTCAT CTCCGACATC 
CGCAACTGTA AAAGTAAAGA AGCTGAAATA AAGAGAATAA ACAAAGAACT GGCAAATATT 
AGATCCAAAT TTAAAGGTGA CAAGGCTCTT GATGGCTACA GTAAAAAGAA GTATGTCTGC 
AAATTGCTCT TCATCTTTCT CCTTGGTCAT GACATTGACT TTGGACACAT GGAAGCTGTG 
AATCTTCTGA GCTCAAACAG ATACACGGAA AAGCAGATTG GCTACCTTTT CATCTCTGTA 
TTGGTGAACT CGAATAGTGA ACTGATCCGG TTGATTAACA ATGCCATCAA GAATGACCTG 
GCCAGCCGCA ATCCTACGTT TATGGGTCTC GCCCTGCACT GCATTGCCAA TGTGGGTAGC 
CGTGAGATGG CGGAGGCCTT CGCCGGAGAG ATCCCCAAGA TCCTCGTGGC AGGAGATACC 
ATGGATAGTG TGAAGCAGAG CGCAGCCCTG TGCTTGTTAC GCCTGTACAG GACATCACCT 
GACCTAGTTC CCATGGGCGA CTGGACATCT CGTGTGGTAC ACCTCCTCAA TGACCAGCAC 
TTGGGGGTAG TGACTGCAGC GACTAGCCTC ATCACCACCC TGGCCCAGAA GAACCCTGAG 
GAGTTCAAGA CCTCTGTCTC GCTAGCTGTC TCGCGACTGA GCAGGATTGT GACGTCTGCA 
TCAACAGATC TTCAGGATTA TACTTACTAC TTTGTCCCGG CTCCTTGGCT GTCAGTCAAG 
CTTCTGAGAC TGCTGCAGTG CTACCCACCC CCAGAAGACC CTGCGGTGCG TGGCCGCCTG 
ACTGAGTGCT TGGAGACCAT CCTGAACAAG GCCCAGGAGC CACCCAAGTC CAAGAAGGTC 
CAGCATTCAA ATGCCAAGAA CGCTGTGCTG TTTGAGGCCA TCAGCCTGAT CATCCACCAT 
GACAGTGAGC CAAACTTGCT CGTCCGCGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACGA ACCTACGCTA CCTGGCTCTG GAGAGCATGT GCACGCTGGC CAGCTCTGAG 
TTCTCCCACG AGGCCGTCAA GACCCACATT GAGACGGTCA TCAATGCCTT GAAGACGGAA 
AGAGACGTGA GTGTGAGGCA GCGGGCAGTG GACCTGCTCT ACGCCATGTG TGACCGGAGC 
AACGCCCAGC AGATTGTGGC TGAGATGCTG AGCTACCTAG AAACGGCTGA CTACTCCATC 
CGAGAGGAAA TTGTGCTGAA GGTGGCCATC CTGGCTGAGA AGTATGCAGT GGACTACACT 
TGGTATGTGG ACACCATCCT CAACCTCATC CGCATCGCGG GCGACTATGT GAGCGAGGAG 
GTGTGGTACC GCGTCATCCA GATTGTCATC AATCGCGATG ATGTGCAGGG CTATGCTGCC 
AAGACGGTAT TTGAGGCATT GCAGGCGCCA GCATGTCATG AGAACTTGGT CAAAGTGGGC 
GGCTACATCC TGGGGGAGTT TGGAAACTTG ATAGCTGGGG ACCCACGATC CAGCCCTCTG 
ATCCAGTTCA ACCTGCTCCA CTCCAAGTTC CACTTGTGCA GCGTCCCCAC TCGGGCACTC 
CTGCTGTCCA CCTACATCAA GTTCGTGAAC CTCTTTCCAG AGGTAAAAGC TACCATTCAG 
GACGTGCTGC GCAGTGACAG CCAGCTGAAG AATGCCGACG TGGAGCTGCA GCAGCGGGCT 
GTGGAGTACC TGCGACTGAG CACTGTCGCC AGCACTGACA TCCTGGCAAC TGTCCTGGAG 
GAAATGCCAC CCTTCCCTGA GCGTGAGTCC TCCATCTTGG CCAAGCTGAA GAAGAAGAAG 
GGCCCAAGCA CAGTGACTGA CCTGGAGGAG ACCAAGCGGG AGCGAAGCAT CGATGTGAAT 
GGGGGCCCTG AACCCGTTCC AGCCAGCACC AGTGCTGCGT CCACACCTTC TCCGTCAGCA 
GACCTGCTGG GTCTGGGGGC CGTGCCCCCT GCTCCCACGG GGCCCCCTCC CTCCTCTGGC 
GGCGGGCTGC TGGTGGACGT GTTCTCAGAC TCAGCTTCTG CAGTCGCACC TCTTGCACCC 
GGCTCTGAAG ACAACTTTGC CAGGTTTGTT TGTAAAAATA ATGGTGTATT GTTCGAAAAC 
CAGCTGCTTC AAATTGGACT TAAGTCAGAA TTTCGGCAGA ATTTAGGCCG AATGTTCATA 
TTTTATGGTA ACAAGACCTC CACGCAGTTC CTAAACTTCA CTCCAACACT AATCTGTGCT 
GACGACCTTC AAACTAATCT GAACCTGCAG ACTAAGCCCG TGGACCCAAC TGTGGATGGG 
GGCGCACAGG TGCAACAAGT GGTCAACATT GAGTGTATAT CTGATTTCAC AGAGGCACCT 
GTCCTCAACA TCCAGTTCAG GTATGGTGGG ACCTTCCAGA ATGTGTCTGT TAAGCTTCCC 
ATCACTCTCA ACAAATTTTT CCAGCCCACA GAAATGGCTT CTCAGGATTT CTTTCAACGT 
TGGAAGCAGT TGAGCAATCC ACAGCAAGAA GTGCAGAATA TCTTCAAAGC AAAGCATCCG 
ATGGACACAG AGATCACTAA GGCAAAGATT ATTGGATTTG GTTCTGCGCT CCTGGAAGAA 
GTTGACCCGA ATCCTGCAAA TTTCGTGGGT GCTGGCATAA TACACACCAA AACCACCCAG 
ATTGGCTGCT TGTTGCGCCT GGAGCCAAAC CTGCAAGCTC AGATGTATAG ACTCACCTTG 
CGTACCAGCA AAGACACCGT CTCTCAGAGA CTATGTGAAT TGCTGTCGGA ACAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap2a2 ( NM_007459.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031485.3 Mus musculus adaptor-related protein complex 2, alpha 2 subunit (Ap2a2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19986
Clone ID Related Accession (Same CDS sequence)	NM_007459.3
Accession Version	NM_007459.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	AP-2 complex subunit alpha-2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY729047.1, AK173054.1 and BC058099.1. On Dec 20, 2007 this sequence version replaced NM_007459.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058099.1, AK128972.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGCCG TATCCAAGGG GGACGGGATG CGAGGCCTAG CGGTCTTCAT CTCCGACATC 
CGCAACTGTA AAAGTAAAGA AGCTGAAATA AAGAGAATAA ACAAAGAACT GGCAAATATT 
AGATCCAAAT TTAAAGGTGA CAAGGCTCTT GATGGCTACA GTAAAAAGAA GTATGTCTGC 
AAATTGCTCT TCATCTTTCT CCTTGGTCAT GACATTGACT TTGGACACAT GGAAGCTGTG 
AATCTTCTGA GCTCAAACAG ATACACGGAA AAGCAGATTG GCTACCTTTT CATCTCTGTA 
TTGGTGAACT CGAATAGTGA ACTGATCCGG TTGATTAACA ATGCCATCAA GAATGACCTG 
GCCAGCCGCA ATCCTACGTT TATGGGTCTC GCCCTGCACT GCATTGCCAA TGTGGGTAGC 
CGTGAGATGG CGGAGGCCTT CGCCGGAGAG ATCCCCAAGA TCCTCGTGGC AGGAGATACC 
ATGGATAGTG TGAAGCAGAG CGCAGCCCTG TGCTTGTTAC GCCTGTACAG GACATCACCT 
GACCTAGTTC CCATGGGCGA CTGGACATCT CGTGTGGTAC ACCTCCTCAA TGACCAGCAC 
TTGGGGGTAG TGACTGCAGC GACTAGCCTC ATCACCACCC TGGCCCAGAA GAACCCTGAG 
GAGTTCAAGA CCTCTGTCTC GCTAGCTGTC TCGCGACTGA GCAGGATTGT GACGTCTGCA 
TCAACAGATC TTCAGGATTA TACTTACTAC TTTGTCCCGG CTCCTTGGCT GTCAGTCAAG 
CTTCTGAGAC TGCTGCAGTG CTACCCACCC CCAGACCCTG CGGTGCGTGG CCGCCTGACT 
GAGTGCTTGG AGACCATCCT GAACAAGGCC CAGGAGCCAC CCAAGTCCAA GAAGGTCCAG 
CATTCAAATG CCAAGAACGC TGTGCTGTTT GAGGCCATCA GCCTGATCAT CCACCATGAC 
AGTGAGCCAA ACTTGCTCGT CCGCGCCTGC AACCAGCTGG GCCAGTTCCT GCAGCACCGG 
GAGACGAACC TACGCTACCT GGCTCTGGAG AGCATGTGCA CGCTGGCCAG CTCTGAGTTC 
TCCCACGAGG CCGTCAAGAC CCACATTGAG ACGGTCATCA ATGCCTTGAA GACGGAAAGA 
GACGTGAGTG TGAGGCAGCG GGCAGTGGAC CTGCTCTACG CCATGTGTGA CCGGAGCAAC 
GCCCAGCAGA TTGTGGCTGA GATGCTGAGC TACCTAGAAA CGGCTGACTA CTCCATCCGA 
GAGGAAATTG TGCTGAAGGT GGCCATCCTG GCTGAGAAGT ATGCAGTGGA CTACACTTGG 
TATGTGGACA CCATCCTCAA CCTCATCCGC ATCGCGGGCG ACTATGTGAG CGAGGAGGTG 
TGGTACCGCG TCATCCAGAT TGTCATCAAT CGCGATGATG TGCAGGGCTA TGCTGCCAAG 
ACGGTATTTG AGGCATTGCA GGCGCCAGCA TGTCATGAGA ACTTGGTCAA AGTGGGCGGC 
TACATCCTGG GGGAGTTTGG AAACTTGATA GCTGGGGACC CACGATCCAG CCCTCTGATC 
CAGTTCAACC TGCTCCACTC CAAGTTCCAC TTGTGCAGCG TCCCCACTCG GGCACTCCTG 
CTGTCCACCT ACATCAAGTT CGTGAACCTC TTTCCAGAGG TAAAAGCTAC CATTCAGGAC 
GTGCTGCGCA GTGACAGCCA GCTGAAGAAT GCCGACGTGG AGCTGCAGCA GCGGGCTGTG 
GAGTACCTGC GACTGAGCAC TGTCGCCAGC ACTGACATCC TGGCAACTGT CCTGGAGGAA 
ATGCCACCCT TCCCTGAGCG TGAGTCCTCC ATCTTGGCCA AGCTGAAGAA GAAGAAGGGC 
CCAAGCACAG TGACTGACCT GGAGGAGACC AAGCGGGAGC GAAGCATCGA TGTGAATGGG 
GGCCCTGAAC CCGTTCCAGC CAGCACCAGT GCTGCGTCCA CACCTTCTCC GTCAGCAGAC 
CTGCTGGGTC TGGGGGCCGT GCCCCCTGCT CCCACGGGGC CCCCTCCCTC CTCTGGCGGC 
GGGCTGCTGG TGGACGTGTT CTCAGACTCA GCTTCTGCAG TCGCACCTCT TGCACCCGGC 
TCTGAAGACA ACTTTGCCAG GTTTGTTTGT AAAAATAATG GTGTATTGTT CGAAAACCAG 
CTGCTTCAAA TTGGACTTAA GTCAGAATTT CGGCAGAATT TAGGCCGAAT GTTCATATTT 
TATGGTAACA AGACCTCCAC GCAGTTCCTA AACTTCACTC CAACACTAAT CTGTGCTGAC 
GACCTTCAAA CTAATCTGAA CCTGCAGACT AAGCCCGTGG ACCCAACTGT GGATGGGGGC 
GCACAGGTGC AACAAGTGGT CAACATTGAG TGTATATCTG ATTTCACAGA GGCACCTGTC 
CTCAACATCC AGTTCAGGTA TGGTGGGACC TTCCAGAATG TGTCTGTTAA GCTTCCCATC 
ACTCTCAACA AATTTTTCCA GCCCACAGAA ATGGCTTCTC AGGATTTCTT TCAACGTTGG 
AAGCAGTTGA GCAATCCACA GCAAGAAGTG CAGAATATCT TCAAAGCAAA GCATCCGATG 
GACACAGAGA TCACTAAGGC AAAGATTATT GGATTTGGTT CTGCGCTCCT GGAAGAAGTT 
GACCCGAATC CTGCAAATTT CGTGGGTGCT GGCATAATAC ACACCAAAAC CACCCAGATT 
GGCTGCTTGT TGCGCCTGGA GCCAAACCTG CAAGCTCAGA TGTATAGACT CACCTTGCGT 
ACCAGCAAAG ACACCGTCTC TCAGAGACTA TGTGAATTGC TGTCGGAACA GTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031485.3
CDS	181..2997
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIANVGSREMAEAFAGEIPKILVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPDPAVRGRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAQQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFNLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKATIQDVLRSDSQLKNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEETKRERSIDVNGGPEPVPASTSAASTPSPSADLLGLGAVPPAPTGPPPSSGGGLLVDVFSDSASAVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICADDLQTNLNLQTKPVDPTVDGGAQVQQVVNIECISDFTEAPVLNIQFRYGGTFQNVSVKLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEITKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKDTVSQRLCELLSEQF

Target ORF information:

RefSeq Version	NM_007459.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor-related protein complex 2, alpha 2 subunit (Ap2a2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007459.3

ORF Insert Sequence:

ATGCCGGCCG TATCCAAGGG GGACGGGATG CGAGGCCTAG CGGTCTTCAT CTCCGACATC 
CGCAACTGTA AAAGTAAAGA AGCTGAAATA AAGAGAATAA ACAAAGAACT GGCAAATATT 
AGATCCAAAT TTAAAGGTGA CAAGGCTCTT GATGGCTACA GTAAAAAGAA GTATGTCTGC 
AAATTGCTCT TCATCTTTCT CCTTGGTCAT GACATTGACT TTGGACACAT GGAAGCTGTG 
AATCTTCTGA GCTCAAACAG ATACACGGAA AAGCAGATTG GCTACCTTTT CATCTCTGTA 
TTGGTGAACT CGAATAGTGA ACTGATCCGG TTGATTAACA ATGCCATCAA GAATGACCTG 
GCCAGCCGCA ATCCTACGTT TATGGGTCTC GCCCTGCACT GCATTGCCAA TGTGGGTAGC 
CGTGAGATGG CGGAGGCCTT CGCCGGAGAG ATCCCCAAGA TCCTCGTGGC AGGAGATACC 
ATGGATAGTG TGAAGCAGAG CGCAGCCCTG TGCTTGTTAC GCCTGTACAG GACATCACCT 
GACCTAGTTC CCATGGGCGA CTGGACATCT CGTGTGGTAC ACCTCCTCAA TGACCAGCAC 
TTGGGGGTAG TGACTGCAGC GACTAGCCTC ATCACCACCC TGGCCCAGAA GAACCCTGAG 
GAGTTCAAGA CCTCTGTCTC GCTAGCTGTC TCGCGACTGA GCAGGATTGT GACGTCTGCA 
TCAACAGATC TTCAGGATTA TACTTACTAC TTTGTCCCGG CTCCTTGGCT GTCAGTCAAG 
CTTCTGAGAC TGCTGCAGTG CTACCCACCC CCAGACCCTG CGGTGCGTGG CCGCCTGACT 
GAGTGCTTGG AGACCATCCT GAACAAGGCC CAGGAGCCAC CCAAGTCCAA GAAGGTCCAG 
CATTCAAATG CCAAGAACGC TGTGCTGTTT GAGGCCATCA GCCTGATCAT CCACCATGAC 
AGTGAGCCAA ACTTGCTCGT CCGCGCCTGC AACCAGCTGG GCCAGTTCCT GCAGCACCGG 
GAGACGAACC TACGCTACCT GGCTCTGGAG AGCATGTGCA CGCTGGCCAG CTCTGAGTTC 
TCCCACGAGG CCGTCAAGAC CCACATTGAG ACGGTCATCA ATGCCTTGAA GACGGAAAGA 
GACGTGAGTG TGAGGCAGCG GGCAGTGGAC CTGCTCTACG CCATGTGTGA CCGGAGCAAC 
GCCCAGCAGA TTGTGGCTGA GATGCTGAGC TACCTAGAAA CGGCTGACTA CTCCATCCGA 
GAGGAAATTG TGCTGAAGGT GGCCATCCTG GCTGAGAAGT ATGCAGTGGA CTACACTTGG 
TATGTGGACA CCATCCTCAA CCTCATCCGC ATCGCGGGCG ACTATGTGAG CGAGGAGGTG 
TGGTACCGCG TCATCCAGAT TGTCATCAAT CGCGATGATG TGCAGGGCTA TGCTGCCAAG 
ACGGTATTTG AGGCATTGCA GGCGCCAGCA TGTCATGAGA ACTTGGTCAA AGTGGGCGGC 
TACATCCTGG GGGAGTTTGG AAACTTGATA GCTGGGGACC CACGATCCAG CCCTCTGATC 
CAGTTCAACC TGCTCCACTC CAAGTTCCAC TTGTGCAGCG TCCCCACTCG GGCACTCCTG 
CTGTCCACCT ACATCAAGTT CGTGAACCTC TTTCCAGAGG TAAAAGCTAC CATTCAGGAC 
GTGCTGCGCA GTGACAGCCA GCTGAAGAAT GCCGACGTGG AGCTGCAGCA GCGGGCTGTG 
GAGTACCTGC GACTGAGCAC TGTCGCCAGC ACTGACATCC TGGCAACTGT CCTGGAGGAA 
ATGCCACCCT TCCCTGAGCG TGAGTCCTCC ATCTTGGCCA AGCTGAAGAA GAAGAAGGGC 
CCAAGCACAG TGACTGACCT GGAGGAGACC AAGCGGGAGC GAAGCATCGA TGTGAATGGG 
GGCCCTGAAC CCGTTCCAGC CAGCACCAGT GCTGCGTCCA CACCTTCTCC GTCAGCAGAC 
CTGCTGGGTC TGGGGGCCGT GCCCCCTGCT CCCACGGGGC CCCCTCCCTC CTCTGGCGGC 
GGGCTGCTGG TGGACGTGTT CTCAGACTCA GCTTCTGCAG TCGCACCTCT TGCACCCGGC 
TCTGAAGACA ACTTTGCCAG GTTTGTTTGT AAAAATAATG GTGTATTGTT CGAAAACCAG 
CTGCTTCAAA TTGGACTTAA GTCAGAATTT CGGCAGAATT TAGGCCGAAT GTTCATATTT 
TATGGTAACA AGACCTCCAC GCAGTTCCTA AACTTCACTC CAACACTAAT CTGTGCTGAC 
GACCTTCAAA CTAATCTGAA CCTGCAGACT AAGCCCGTGG ACCCAACTGT GGATGGGGGC 
GCACAGGTGC AACAAGTGGT CAACATTGAG TGTATATCTG ATTTCACAGA GGCACCTGTC 
CTCAACATCC AGTTCAGGTA TGGTGGGACC TTCCAGAATG TGTCTGTTAA GCTTCCCATC 
ACTCTCAACA AATTTTTCCA GCCCACAGAA ATGGCTTCTC AGGATTTCTT TCAACGTTGG 
AAGCAGTTGA GCAATCCACA GCAAGAAGTG CAGAATATCT TCAAAGCAAA GCATCCGATG 
GACACAGAGA TCACTAAGGC AAAGATTATT GGATTTGGTT CTGCGCTCCT GGAAGAAGTT 
GACCCGAATC CTGCAAATTT CGTGGGTGCT GGCATAATAC ACACCAAAAC CACCCAGATT 
GGCTGCTTGT TGCGCCTGGA GCCAAACCTG CAAGCTCAGA TGTATAGACT CACCTTGCGT 
ACCAGCAAAG ACACCGTCTC TCAGAGACTA TGTGAATTGC TGTCGGAACA GTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap2a2 ( XM_006536155.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536218.1 Mus musculus adaptor-related protein complex 2, alpha 2 subunit (Ap2a2), transcript variant X1, mRNA.

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CloneID	OMu40040
Clone ID Related Accession (Same CDS sequence)	XM_006536155.4 , XM_006536155.3
Accession Version	XM_006536155.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	AP-2 complex subunit alpha-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006536155.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGACC GGAGCAACGC CCAGCAGATT GTGGCTGAGA TGCTGAGCTA CCTAGAAACG 
GCTGACTACT CCATCCGAGA GGAAATTGTG CTGAAGGTGG CCATCCTGGC TGAGAAGTAT 
GCAGTGGACT ACACTTGGTA TGTGGACACC ATCCTCAACC TCATCCGCAT CGCGGGCGAC 
TATGTGAGCG AGGAGGTGTG GTACCGCGTC ATCCAGATTG TCATCAATCG CGATGATGTG 
CAGGGCTATG CTGCCAAGAC GGTATTTGAG GCATTGCAGG CGCCAGCATG TCATGAGAAC 
TTGGTCAAAG TGGGCGGCTA CATCCTGGGG GAGTTTGGAA ACTTGATAGC TGGGGACCCA 
CGATCCAGCC CTCTGATCCA GTTCAACCTG CTCCACTCCA AGTTCCACTT GTGCAGCGTC 
CCCACTCGGG CACTCCTGCT GTCCACCTAC ATCAAGTTCG TGAACCTCTT TCCAGAGGTA 
AAAGCTACCA TTCAGGACGT GCTGCGCAGT GACAGCCAGC TGAAGAATGC CGACGTGGAG 
CTGCAGCAGC GGGCTGTGGA GTACCTGCGA CTGAGCACTG TCGCCAGCAC TGACATCCTG 
GCAACTGTCC TGGAGGAAAT GCCACCCTTC CCTGAGCGTG AGTCCTCCAT CTTGGCCAAG 
CTGAAGAAGA AGAAGGGCCC AAGCACAGTG ACTGACCTGG AGGAGACCAA GCGGGAGCGA 
AGCATCGATG TGAATGGGGG CCCTGAACCC GTTCCAGCCA GCACCAGTGC TGCGTCCACA 
CCTTCTCCGT CAGCAGACCT GCTGGGTCTG GGGGCCGTGC CCCCTGCTCC CACGGGGCCC 
CCTCCCTCCT CTGGCGGCGG GCTGCTGGTG GACGTGTTCT CAGACTCAGC TTCTGCAGTC 
GCACCTCTTG CACCCGGCTC TGAAGACAAC TTTGCCAGGT TTGTTTGTAA AAATAATGGT 
GTATTGTTCG AAAACCAGCT GCTTCAAATT GGACTTAAGT CAGAATTTCG GCAGAATTTA 
GGCCGAATGT TCATATTTTA TGGTAACAAG ACCTCCACGC AGTTCCTAAA CTTCACTCCA 
ACACTAATCT GTGCTGACGA CCTTCAAACT AATCTGAACC TGCAGACTAA GCCCGTGGAC 
CCAACTGTGG ATGGGGGCGC ACAGGTGCAA CAAGTGGTCA ACATTGAGTG TATATCTGAT 
TTCACAGAGG CACCTGTCCT CAACATCCAG TTCAGGTATG GTGGGACCTT CCAGAATGTG 
TCTGTTAAGC TTCCCATCAC TCTCAACAAA TTTTTCCAGC CCACAGAAAT GGCTTCTCAG 
GATTTCTTTC AACGTTGGAA GCAGTTGAGC AATCCACAGC AAGAAGTGCA GAATATCTTC 
AAAGCAAAGC ATCCGATGGA CACAGAGATC ACTAAGGCAA AGATTATTGG ATTTGGTTCT 
GCGCTCCTGG AAGAAGTTGA CCCGAATCCT GCAAATTTCG TGGGTGCTGG CATAATACAC 
ACCAAAACCA CCCAGATTGG CTGCTTGTTG CGCCTGGAGC CAAACCTGCA AGCTCAGATG 
TATAGACTCA CCTTGCGTAC CAGCAAAGAC ACCGTCTCTC AGAGACTATG TGAATTGCTG 
TCGGAACAGT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536218.1
CDS	120..1754
Translation	MCDRSNAQQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFNLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKATIQDVLRSDSQLKNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEETKRERSIDVNGGPEPVPASTSAASTPSPSADLLGLGAVPPAPTGPPPSSGGGLLVDVFSDSASAVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICADDLQTNLNLQTKPVDPTVDGGAQVQQVVNIECISDFTEAPVLNIQFRYGGTFQNVSVKLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEITKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKDTVSQRLCELLSEQF

Target ORF information:

RefSeq Version	XM_006536155.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor-related protein complex 2, alpha 2 subunit (Ap2a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536155.4

ORF Insert Sequence:

ATGTGTGACC GGAGCAACGC CCAGCAGATT GTGGCTGAGA TGCTGAGCTA CCTAGAAACG 
GCTGACTACT CCATCCGAGA GGAAATTGTG CTGAAGGTGG CCATCCTGGC TGAGAAGTAT 
GCAGTGGACT ACACTTGGTA TGTGGACACC ATCCTCAACC TCATCCGCAT CGCGGGCGAC 
TATGTGAGCG AGGAGGTGTG GTACCGCGTC ATCCAGATTG TCATCAATCG CGATGATGTG 
CAGGGCTATG CTGCCAAGAC GGTATTTGAG GCATTGCAGG CGCCAGCATG TCATGAGAAC 
TTGGTCAAAG TGGGCGGCTA CATCCTGGGG GAGTTTGGAA ACTTGATAGC TGGGGACCCA 
CGATCCAGCC CTCTGATCCA GTTCAACCTG CTCCACTCCA AGTTCCACTT GTGCAGCGTC 
CCCACTCGGG CACTCCTGCT GTCCACCTAC ATCAAGTTCG TGAACCTCTT TCCAGAGGTA 
AAAGCTACCA TTCAGGACGT GCTGCGCAGT GACAGCCAGC TGAAGAATGC CGACGTGGAG 
CTGCAGCAGC GGGCTGTGGA GTACCTGCGA CTGAGCACTG TCGCCAGCAC TGACATCCTG 
GCAACTGTCC TGGAGGAAAT GCCACCCTTC CCTGAGCGTG AGTCCTCCAT CTTGGCCAAG 
CTGAAGAAGA AGAAGGGCCC AAGCACAGTG ACTGACCTGG AGGAGACCAA GCGGGAGCGA 
AGCATCGATG TGAATGGGGG CCCTGAACCC GTTCCAGCCA GCACCAGTGC TGCGTCCACA 
CCTTCTCCGT CAGCAGACCT GCTGGGTCTG GGGGCCGTGC CCCCTGCTCC CACGGGGCCC 
CCTCCCTCCT CTGGCGGCGG GCTGCTGGTG GACGTGTTCT CAGACTCAGC TTCTGCAGTC 
GCACCTCTTG CACCCGGCTC TGAAGACAAC TTTGCCAGGT TTGTTTGTAA AAATAATGGT 
GTATTGTTCG AAAACCAGCT GCTTCAAATT GGACTTAAGT CAGAATTTCG GCAGAATTTA 
GGCCGAATGT TCATATTTTA TGGTAACAAG ACCTCCACGC AGTTCCTAAA CTTCACTCCA 
ACACTAATCT GTGCTGACGA CCTTCAAACT AATCTGAACC TGCAGACTAA GCCCGTGGAC 
CCAACTGTGG ATGGGGGCGC ACAGGTGCAA CAAGTGGTCA ACATTGAGTG TATATCTGAT 
TTCACAGAGG CACCTGTCCT CAACATCCAG TTCAGGTATG GTGGGACCTT CCAGAATGTG 
TCTGTTAAGC TTCCCATCAC TCTCAACAAA TTTTTCCAGC CCACAGAAAT GGCTTCTCAG 
GATTTCTTTC AACGTTGGAA GCAGTTGAGC AATCCACAGC AAGAAGTGCA GAATATCTTC 
AAAGCAAAGC ATCCGATGGA CACAGAGATC ACTAAGGCAA AGATTATTGG ATTTGGTTCT 
GCGCTCCTGG AAGAAGTTGA CCCGAATCCT GCAAATTTCG TGGGTGCTGG CATAATACAC 
ACCAAAACCA CCCAGATTGG CTGCTTGTTG CGCCTGGAGC CAAACCTGCA AGCTCAGATG 
TATAGACTCA CCTTGCGTAC CAGCAAAGAC ACCGTCTCTC AGAGACTATG TGAATTGCTG 
TCGGAACAGT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

APache Is an AP2-Interacting Protein Involved in Synaptic Vesicle Trafficking and Neuronal Development.
Cell reports21(12)3596-3611(2017 Dec)
Piccini A,Castroflorio E,Valente P,Guarnieri FC,Aprile D,Michetti C,Bramini M,Giansante G,Pinto B,Savardi A,Cesca F,Bachi A,Cattaneo A,Wren JD,Fassio A,Valtorta F,Benfenati F,Gioved? S

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Ap2a2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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