AT5G22780 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT5G22780
Entrez Gene ID	832341
Full Name	Adaptor protein complex AP-2, alpha subunit
Synonyms	K5A21.2,K5A21_2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_122184.4	NP_197670.1	Adaptor protein complex AP-2, alpha subunit
NM_001343765.1	NP_001330127.1	Adaptor protein complex AP-2, alpha subunit

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	AP2A2	NP_001012914.1
Drosophila melanogaster (fruit fly)	alpha-Adaptin	NP_476819.2
Caenorhabditis elegans (roundworm)	apa-2	NP_509572.1
Neurospora crassa	NCU03440	XP_956726.1
Arabidopsis thaliana (thale cress)	alpha-ADR	NP_197669.1
Pan troglodytes (chimpanzee)	AP2A2	XP_003951843.1
Rattus norvegicus (Norway rat)	Ap2a2	NP_112270.2
Anopheles gambiae (African malaria mosquito)	AP2A_ANOGA	XP_310153.4
Arabidopsis thaliana (thale cress)	AT5G22780	NP_197670.1
Homo sapiens (human)	AP2A2	NP_001229766.1
	AP2A2	XP_001116794.2
Schizosaccharomyces pombe (fission yeast)	apl3	NP_595595.1
Magnaporthe oryzae (rice blast fungus)	MGG_09493	XP_003712251.1
Canis lupus familiaris (dog)	AP2A2	XP_005631788.1
Xenopus tropicalis (tropical clawed frog)	ap2a2	NP_001001209.1
Bos taurus (cattle)	AP2A2	NP_001069170.1
Mus musculus (house mouse)	Ap2a2	NP_031485.3

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT5G22780 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT5G22780 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb19266	NM_122184.4 Latest version!	Arabidopsis thaliana Adaptor protein complex AP-2, alpha subunit (AT5G22780), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb19266	NM_001343765.1 Latest version!	Arabidopsis thaliana Adaptor protein complex AP-2, alpha subunit (AT5G22780), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT5G22780 ( NM_122184.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_197670.1 Arabidopsis thaliana Adaptor protein complex AP-2, alpha subunit (AT5G22780), mRNA.

Price & Availability↑

CloneID	OAb19266
Clone ID Related Accession (Same CDS sequence)	NM_122184.4 , NM_001343765.1
Accession Version	NM_122184.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Adaptor protein complex AP-2, alpha subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_122184.3.

ATGACCGGAA TGAGAGGTCT CTCCGTATTT ATCAGCGACG TTCGTAATTG TCAGAACAAG 
GAAGCGGAGA GACTACGAGT TGATAAGGAG CTCGGAAACA TTCGTACATG CTTCAAGAAT 
GAGAAGGTCT TAACACCATA CAAAAAGAAG AAATATGTCT GGAAAATGCT TTATATACAT 
ATGCTAGGCT ACGATGTGGA TTTTGGCCAT ATGGAGGCAG TTTCTCTTAT ATCTGCGCCA 
AAGTATCCAG AAAAGCAGGT TGGCTACATT GTTACATCTT GCTTGCTCAA TGAGAACCAT 
GACTTTTTGA AGTTGGCTAT AAATACAGTG CGGAACGATA TTATTGGTCG GAATGAGACT 
TTCCAATGCT TAGCACTGAC CTTGGTTGGA AATATTGGTG GAAGAGATTT TGCTGAATCA 
TTGGCACCTG ATGTTCAAAA GCTACTTATT TCTAGTAGTT GCAGACCTCT CGTAAGGAAA 
AAAGCGGCAC TGTGCCTACT GCGCTTATTC CGAAAAAATC CGGATGCTGT CAACGTTGAT 
GGCTGGGCGG ATCGTATGGC ACAACTTTTG GATGAACGAG ATCTTGGTGT CTTGACGTCT 
TCTACGAGTC TTCTTGTAGC TCTGGTATCT AACAATCATG AGGCGTACTC AAGTTGTCTA 
CCTAAATGTG TCAAAATATT AGAACGCCTT GCTAGGAACC AGGATGTTCC TCAGGAGTAC 
ACATACTATG GCATCCCATC TCCTTGGCTC CAGGTTAAGG CAATGAGGGC TCTTCAATAT 
TTTCCAACCA TTGAAGATCC CAGTACCAGA AAAGCTCTCT TTGAGGTCCT TCAGCGAATA 
TTGATGGGCA CAGATGTTGT GAAAAATGTG AATAAAAACA ATGCGTCTCA TGCCGTGCTG 
TTTGAAGCTC TCTCCCTGGT CATGCATCTT GATGCTGAGA AGGAAATGAT GTCACAGTGT 
GTTGCTCTTC TTGGCAAGTT TATTTCTGTC CGTGAGCCCA ACATCAGATA TCTAGGCTTG 
GAAAATATGA CGCGAATGCT GATGGTCACA GATGTTCAAG ATATCATAAA GAAGCATCAG 
TCTCAGATAA TCACCTCGTT GAAAGACCCT GACATCAGTA TTCGTAGACG CGCTCTTGAT 
TTGCTATATG GGATGTGCGA TGTGTCAAAT GCAAAGGATA TAGTTGAAGA GTTGTTGCAG 
TATCTTAGCA CAGCAGAATT TTCTATGCGG GAGGAATTAT CACTTAAAGC TGCTATTCTT 
GCTGAGAAGT TTGCTCCTGA TCTTTCATGG TATGTAGATG TGATCCTTCA GTTGATCGAC 
AAGGCTGGGG ATTTTGTTAG CGATGACATT TGGTTTCGTG TGGTTCAGTT TGTAACAAAT 
AATGAAGACC TGCAGCCTTA TGCGGCATCA AAAGCAAGAG AATATATGGA CAAAATTGCA 
ATCCATGAGA CCATGGTGAA GGTCAGTGCT TATATTTTGG GGGAGTATGG TCACCTTTTA 
GCTAGACAAC CTGGATGCAG TGCAAGTGAA CTCTTTAGCA TCTTACATGA GAAGCTTCCT 
ACAGTATCGA CTCCCACCAT TCCTATTCTT CTTTCTACAT ATGCAAAGCT TCTTATGCAC 
GCTCAACCCC CGGATCCAGA ACTGCAAAAA AAAGTTTGGG CTGTATTTAA GAAATATGAG 
AGTTGTATCG ATGTGGAGAT ACAACAGAGG GCAGTTGAAT ATTTTGAATT GAGTAAAAAA 
GGGCCTGCCT TTATGGATGT ATTGGCTGAG ATGCCAAAAT TTCCGGAGCG CCAGTCTTCA 
TTGATTAAAA AGGCAGAAAA CGTTGAAGAT ACCGCAGACC AGAGTGCCAT CAAACTTAGA 
GCACAACAAC AGCCTTCAAA TGCTATTGTT TTGGCTGACC CACAGCCTGT TAATGGGGCA 
CCACCACCTT TGAAGGTTCC AATTTTAAGT GGAAGCACGG ACCCTGAGTC AGTGGCTCGA 
TCTCTCTCAC ATCCCAATGG AACTTTAAGT AATATAGATC CACAAACTCC ATCACCTGAT 
CTCCTTAGTG ACCTACTCGG TCCTCTTGCA ATAGAGGCTC CACCTGGAGC TGTCTCATAT 
GAACAACATG GTCCCGTAGG GGCAGAAGGA GTTCCAGATG AAATTGATGG TTCCGCCATT 
GTACCTGTTG AAGAGCAAAC AAATACAGTG GAGCTTATCG GAAATATTGC TGAGAGATTT 
CATGCTTTAT GTTTGAAAGA CAGTGGTGTC TTATACGAGG ATCCTCATAT CCAGATTGGC 
ATCAAAGCTG AATGGAGAGG ACACCATGGG AGACTTGTTC TCTTCATGGG AAACAAGAAT 
ACTTCTCCAC TTACATCAGT TCAAGCTCTG ATACTACCCC CTGCCCACTT AAGACTGGAT 
CTGTCTCCAG TACCTGATAC AATTCCTCCT CGAGCACAGG TACAATCTCC GCTTGAAGTG 
ATGAACATCC GCCCTAGTAG GGATGTTGCA GTTTTGGATT TCTCCTACAA GTTTGGTACA 
AACGTGGTCA GTGCCAAATT ACGCATCCCG GCAACCTTGA ATAAGTTTCT TCAACCTCTA 
CAATTGACAT CTGAAGAGTT CTTCCCTCAA TGGAGAGCAA TCTCAGGGCC ACCCTTGAAA 
CTTCAGGAAG TGGTAAGAGG TGTAAGACCT CTAGCTCTTC CGGAAATGGC AAATCTGTTC 
AACAGTTTTC ATGTGACGAT ATGCCCTGGG CTTGATCCAA ACCCTAATAA TCTAGTGGCA 
AGCACAACCT TCTACTCTGA AACTACAGGA GCCATGCTCT GTTTGGCAAG AATTGAAACA 
GACCCAGCGG ACAGAACCCA ACTACGATTG ACAGTAGGCT CGGGAGATCC TACCCTTACA 
TTTGAGTTAA AAGAATTCAT CAAGGAACAA TTAATTACTA TTCCAATGGG ATCTCGAGCT 
CTAGTTCCAG CAGCAGGTCC AGCACCATCA CCAGCAGTTC AACCGCCTAG CCCAGCGGCT 
TTAGCTGATG ATCCAGGAGC TATGCTTGCT GGCTTACTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_197670.1
CDS	286..3327
Translation	MTGMRGLSVFISDVRNCQNKEAERLRVDKELGNIRTCFKNEKVLTPYKKKKYVWKMLYIHMLGYDVDFGHMEAVSLISAPKYPEKQVGYIVTSCLLNENHDFLKLAINTVRNDIIGRNETFQCLALTLVGNIGGRDFAESLAPDVQKLLISSSCRPLVRKKAALCLLRLFRKNPDAVNVDGWADRMAQLLDERDLGVLTSSTSLLVALVSNNHEAYSSCLPKCVKILERLARNQDVPQEYTYYGIPSPWLQVKAMRALQYFPTIEDPSTRKALFEVLQRILMGTDVVKNVNKNNASHAVLFEALSLVMHLDAEKEMMSQCVALLGKFISVREPNIRYLGLENMTRMLMVTDVQDIIKKHQSQIITSLKDPDISIRRRALDLLYGMCDVSNAKDIVEELLQYLSTAEFSMREELSLKAAILAEKFAPDLSWYVDVILQLIDKAGDFVSDDIWFRVVQFVTNNEDLQPYAASKAREYMDKIAIHETMVKVSAYILGEYGHLLARQPGCSASELFSILHEKLPTVSTPTIPILLSTYAKLLMHAQPPDPELQKKVWAVFKKYESCIDVEIQQRAVEYFELSKKGPAFMDVLAEMPKFPERQSSLIKKAENVEDTADQSAIKLRAQQQPSNAIVLADPQPVNGAPPPLKVPILSGSTDPESVARSLSHPNGTLSNIDPQTPSPDLLSDLLGPLAIEAPPGAVSYEQHGPVGAEGVPDEIDGSAIVPVEEQTNTVELIGNIAERFHALCLKDSGVLYEDPHIQIGIKAEWRGHHGRLVLFMGNKNTSPLTSVQALILPPAHLRLDLSPVPDTIPPRAQVQSPLEVMNIRPSRDVAVLDFSYKFGTNVVSAKLRIPATLNKFLQPLQLTSEEFFPQWRAISGPPLKLQEVVRGVRPLALPEMANLFNSFHVTICPGLDPNPNNLVASTTFYSETTGAMLCLARIETDPADRTQLRLTVGSGDPTLTFELKEFIKEQLITIPMGSRALVPAAGPAPSPAVQPPSPAALADDPGAMLAGLL

Target ORF information:

RefSeq Version	NM_122184.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Adaptor protein complex AP-2, alpha subunit (AT5G22780), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_122184.4

ORF Insert Sequence:

ATGACCGGAA TGAGAGGTCT CTCCGTATTT ATCAGCGACG TTCGTAATTG TCAGAACAAG 
GAAGCGGAGA GACTACGAGT TGATAAGGAG CTCGGAAACA TTCGTACATG CTTCAAGAAT 
GAGAAGGTCT TAACACCATA CAAAAAGAAG AAATATGTCT GGAAAATGCT TTATATACAT 
ATGCTAGGCT ACGATGTGGA TTTTGGCCAT ATGGAGGCAG TTTCTCTTAT ATCTGCGCCA 
AAGTATCCAG AAAAGCAGGT TGGCTACATT GTTACATCTT GCTTGCTCAA TGAGAACCAT 
GACTTTTTGA AGTTGGCTAT AAATACAGTG CGGAACGATA TTATTGGTCG GAATGAGACT 
TTCCAATGCT TAGCACTGAC CTTGGTTGGA AATATTGGTG GAAGAGATTT TGCTGAATCA 
TTGGCACCTG ATGTTCAAAA GCTACTTATT TCTAGTAGTT GCAGACCTCT CGTAAGGAAA 
AAAGCGGCAC TGTGCCTACT GCGCTTATTC CGAAAAAATC CGGATGCTGT CAACGTTGAT 
GGCTGGGCGG ATCGTATGGC ACAACTTTTG GATGAACGAG ATCTTGGTGT CTTGACGTCT 
TCTACGAGTC TTCTTGTAGC TCTGGTATCT AACAATCATG AGGCGTACTC AAGTTGTCTA 
CCTAAATGTG TCAAAATATT AGAACGCCTT GCTAGGAACC AGGATGTTCC TCAGGAGTAC 
ACATACTATG GCATCCCATC TCCTTGGCTC CAGGTTAAGG CAATGAGGGC TCTTCAATAT 
TTTCCAACCA TTGAAGATCC CAGTACCAGA AAAGCTCTCT TTGAGGTCCT TCAGCGAATA 
TTGATGGGCA CAGATGTTGT GAAAAATGTG AATAAAAACA ATGCGTCTCA TGCCGTGCTG 
TTTGAAGCTC TCTCCCTGGT CATGCATCTT GATGCTGAGA AGGAAATGAT GTCACAGTGT 
GTTGCTCTTC TTGGCAAGTT TATTTCTGTC CGTGAGCCCA ACATCAGATA TCTAGGCTTG 
GAAAATATGA CGCGAATGCT GATGGTCACA GATGTTCAAG ATATCATAAA GAAGCATCAG 
TCTCAGATAA TCACCTCGTT GAAAGACCCT GACATCAGTA TTCGTAGACG CGCTCTTGAT 
TTGCTATATG GGATGTGCGA TGTGTCAAAT GCAAAGGATA TAGTTGAAGA GTTGTTGCAG 
TATCTTAGCA CAGCAGAATT TTCTATGCGG GAGGAATTAT CACTTAAAGC TGCTATTCTT 
GCTGAGAAGT TTGCTCCTGA TCTTTCATGG TATGTAGATG TGATCCTTCA GTTGATCGAC 
AAGGCTGGGG ATTTTGTTAG CGATGACATT TGGTTTCGTG TGGTTCAGTT TGTAACAAAT 
AATGAAGACC TGCAGCCTTA TGCGGCATCA AAAGCAAGAG AATATATGGA CAAAATTGCA 
ATCCATGAGA CCATGGTGAA GGTCAGTGCT TATATTTTGG GGGAGTATGG TCACCTTTTA 
GCTAGACAAC CTGGATGCAG TGCAAGTGAA CTCTTTAGCA TCTTACATGA GAAGCTTCCT 
ACAGTATCGA CTCCCACCAT TCCTATTCTT CTTTCTACAT ATGCAAAGCT TCTTATGCAC 
GCTCAACCCC CGGATCCAGA ACTGCAAAAA AAAGTTTGGG CTGTATTTAA GAAATATGAG 
AGTTGTATCG ATGTGGAGAT ACAACAGAGG GCAGTTGAAT ATTTTGAATT GAGTAAAAAA 
GGGCCTGCCT TTATGGATGT ATTGGCTGAG ATGCCAAAAT TTCCGGAGCG CCAGTCTTCA 
TTGATTAAAA AGGCAGAAAA CGTTGAAGAT ACCGCAGACC AGAGTGCCAT CAAACTTAGA 
GCACAACAAC AGCCTTCAAA TGCTATTGTT TTGGCTGACC CACAGCCTGT TAATGGGGCA 
CCACCACCTT TGAAGGTTCC AATTTTAAGT GGAAGCACGG ACCCTGAGTC AGTGGCTCGA 
TCTCTCTCAC ATCCCAATGG AACTTTAAGT AATATAGATC CACAAACTCC ATCACCTGAT 
CTCCTTAGTG ACCTACTCGG TCCTCTTGCA ATAGAGGCTC CACCTGGAGC TGTCTCATAT 
GAACAACATG GTCCCGTAGG GGCAGAAGGA GTTCCAGATG AAATTGATGG TTCCGCCATT 
GTACCTGTTG AAGAGCAAAC AAATACAGTG GAGCTTATCG GAAATATTGC TGAGAGATTT 
CATGCTTTAT GTTTGAAAGA CAGTGGTGTC TTATACGAGG ATCCTCATAT CCAGATTGGC 
ATCAAAGCTG AATGGAGAGG ACACCATGGG AGACTTGTTC TCTTCATGGG AAACAAGAAT 
ACTTCTCCAC TTACATCAGT TCAAGCTCTG ATACTACCCC CTGCCCACTT AAGACTGGAT 
CTGTCTCCAG TACCTGATAC AATTCCTCCT CGAGCACAGG TACAATCTCC GCTTGAAGTG 
ATGAACATCC GCCCTAGTAG GGATGTTGCA GTTTTGGATT TCTCCTACAA GTTTGGTACA 
AACGTGGTCA GTGCCAAATT ACGCATCCCG GCAACCTTGA ATAAGTTTCT TCAACCTCTA 
CAATTGACAT CTGAAGAGTT CTTCCCTCAA TGGAGAGCAA TCTCAGGGCC ACCCTTGAAA 
CTTCAGGAAG TGGTAAGAGG TGTAAGACCT CTAGCTCTTC CGGAAATGGC AAATCTGTTC 
AACAGTTTTC ATGTGACGAT ATGCCCTGGG CTTGATCCAA ACCCTAATAA TCTAGTGGCA 
AGCACAACCT TCTACTCTGA AACTACAGGA GCCATGCTCT GTTTGGCAAG AATTGAAACA 
GACCCAGCGG ACAGAACCCA ACTACGATTG ACAGTAGGCT CGGGAGATCC TACCCTTACA 
TTTGAGTTAA AAGAATTCAT CAAGGAACAA TTAATTACTA TTCCAATGGG ATCTCGAGCT 
CTAGTTCCAG CAGCAGGTCC AGCACCATCA CCAGCAGTTC AACCGCCTAG CCCAGCGGCT 
TTAGCTGATG ATCCAGGAGC TATGCTTGCT GGCTTACTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT5G22780 ( NM_001343765.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001330127.1 Arabidopsis thaliana Adaptor protein complex AP-2, alpha subunit (AT5G22780), mRNA.

Price & Availability↑

CloneID	OAb19266
Clone ID Related Accession (Same CDS sequence)	NM_122184.4 , NM_001343765.1
Accession Version	NM_001343765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Adaptor protein complex AP-2, alpha subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGACCGGAA TGAGAGGTCT CTCCGTATTT ATCAGCGACG TTCGTAATTG TCAGAACAAG 
GAAGCGGAGA GACTACGAGT TGATAAGGAG CTCGGAAACA TTCGTACATG CTTCAAGAAT 
GAGAAGGTCT TAACACCATA CAAAAAGAAG AAATATGTCT GGAAAATGCT TTATATACAT 
ATGCTAGGCT ACGATGTGGA TTTTGGCCAT ATGGAGGCAG TTTCTCTTAT ATCTGCGCCA 
AAGTATCCAG AAAAGCAGGT TGGCTACATT GTTACATCTT GCTTGCTCAA TGAGAACCAT 
GACTTTTTGA AGTTGGCTAT AAATACAGTG CGGAACGATA TTATTGGTCG GAATGAGACT 
TTCCAATGCT TAGCACTGAC CTTGGTTGGA AATATTGGTG GAAGAGATTT TGCTGAATCA 
TTGGCACCTG ATGTTCAAAA GCTACTTATT TCTAGTAGTT GCAGACCTCT CGTAAGGAAA 
AAAGCGGCAC TGTGCCTACT GCGCTTATTC CGAAAAAATC CGGATGCTGT CAACGTTGAT 
GGCTGGGCGG ATCGTATGGC ACAACTTTTG GATGAACGAG ATCTTGGTGT CTTGACGTCT 
TCTACGAGTC TTCTTGTAGC TCTGGTATCT AACAATCATG AGGCGTACTC AAGTTGTCTA 
CCTAAATGTG TCAAAATATT AGAACGCCTT GCTAGGAACC AGGATGTTCC TCAGGAGTAC 
ACATACTATG GCATCCCATC TCCTTGGCTC CAGGTTAAGG CAATGAGGGC TCTTCAATAT 
TTTCCAACCA TTGAAGATCC CAGTACCAGA AAAGCTCTCT TTGAGGTCCT TCAGCGAATA 
TTGATGGGCA CAGATGTTGT GAAAAATGTG AATAAAAACA ATGCGTCTCA TGCCGTGCTG 
TTTGAAGCTC TCTCCCTGGT CATGCATCTT GATGCTGAGA AGGAAATGAT GTCACAGTGT 
GTTGCTCTTC TTGGCAAGTT TATTTCTGTC CGTGAGCCCA ACATCAGATA TCTAGGCTTG 
GAAAATATGA CGCGAATGCT GATGGTCACA GATGTTCAAG ATATCATAAA GAAGCATCAG 
TCTCAGATAA TCACCTCGTT GAAAGACCCT GACATCAGTA TTCGTAGACG CGCTCTTGAT 
TTGCTATATG GGATGTGCGA TGTGTCAAAT GCAAAGGATA TAGTTGAAGA GTTGTTGCAG 
TATCTTAGCA CAGCAGAATT TTCTATGCGG GAGGAATTAT CACTTAAAGC TGCTATTCTT 
GCTGAGAAGT TTGCTCCTGA TCTTTCATGG TATGTAGATG TGATCCTTCA GTTGATCGAC 
AAGGCTGGGG ATTTTGTTAG CGATGACATT TGGTTTCGTG TGGTTCAGTT TGTAACAAAT 
AATGAAGACC TGCAGCCTTA TGCGGCATCA AAAGCAAGAG AATATATGGA CAAAATTGCA 
ATCCATGAGA CCATGGTGAA GGTCAGTGCT TATATTTTGG GGGAGTATGG TCACCTTTTA 
GCTAGACAAC CTGGATGCAG TGCAAGTGAA CTCTTTAGCA TCTTACATGA GAAGCTTCCT 
ACAGTATCGA CTCCCACCAT TCCTATTCTT CTTTCTACAT ATGCAAAGCT TCTTATGCAC 
GCTCAACCCC CGGATCCAGA ACTGCAAAAA AAAGTTTGGG CTGTATTTAA GAAATATGAG 
AGTTGTATCG ATGTGGAGAT ACAACAGAGG GCAGTTGAAT ATTTTGAATT GAGTAAAAAA 
GGGCCTGCCT TTATGGATGT ATTGGCTGAG ATGCCAAAAT TTCCGGAGCG CCAGTCTTCA 
TTGATTAAAA AGGCAGAAAA CGTTGAAGAT ACCGCAGACC AGAGTGCCAT CAAACTTAGA 
GCACAACAAC AGCCTTCAAA TGCTATTGTT TTGGCTGACC CACAGCCTGT TAATGGGGCA 
CCACCACCTT TGAAGGTTCC AATTTTAAGT GGAAGCACGG ACCCTGAGTC AGTGGCTCGA 
TCTCTCTCAC ATCCCAATGG AACTTTAAGT AATATAGATC CACAAACTCC ATCACCTGAT 
CTCCTTAGTG ACCTACTCGG TCCTCTTGCA ATAGAGGCTC CACCTGGAGC TGTCTCATAT 
GAACAACATG GTCCCGTAGG GGCAGAAGGA GTTCCAGATG AAATTGATGG TTCCGCCATT 
GTACCTGTTG AAGAGCAAAC AAATACAGTG GAGCTTATCG GAAATATTGC TGAGAGATTT 
CATGCTTTAT GTTTGAAAGA CAGTGGTGTC TTATACGAGG ATCCTCATAT CCAGATTGGC 
ATCAAAGCTG AATGGAGAGG ACACCATGGG AGACTTGTTC TCTTCATGGG AAACAAGAAT 
ACTTCTCCAC TTACATCAGT TCAAGCTCTG ATACTACCCC CTGCCCACTT AAGACTGGAT 
CTGTCTCCAG TACCTGATAC AATTCCTCCT CGAGCACAGG TACAATCTCC GCTTGAAGTG 
ATGAACATCC GCCCTAGTAG GGATGTTGCA GTTTTGGATT TCTCCTACAA GTTTGGTACA 
AACGTGGTCA GTGCCAAATT ACGCATCCCG GCAACCTTGA ATAAGTTTCT TCAACCTCTA 
CAATTGACAT CTGAAGAGTT CTTCCCTCAA TGGAGAGCAA TCTCAGGGCC ACCCTTGAAA 
CTTCAGGAAG TGGTAAGAGG TGTAAGACCT CTAGCTCTTC CGGAAATGGC AAATCTGTTC 
AACAGTTTTC ATGTGACGAT ATGCCCTGGG CTTGATCCAA ACCCTAATAA TCTAGTGGCA 
AGCACAACCT TCTACTCTGA AACTACAGGA GCCATGCTCT GTTTGGCAAG AATTGAAACA 
GACCCAGCGG ACAGAACCCA ACTACGATTG ACAGTAGGCT CGGGAGATCC TACCCTTACA 
TTTGAGTTAA AAGAATTCAT CAAGGAACAA TTAATTACTA TTCCAATGGG ATCTCGAGCT 
CTAGTTCCAG CAGCAGGTCC AGCACCATCA CCAGCAGTTC AACCGCCTAG CCCAGCGGCT 
TTAGCTGATG ATCCAGGAGC TATGCTTGCT GGCTTACTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001330127.1
CDS	270..3311
Translation	MTGMRGLSVFISDVRNCQNKEAERLRVDKELGNIRTCFKNEKVLTPYKKKKYVWKMLYIHMLGYDVDFGHMEAVSLISAPKYPEKQVGYIVTSCLLNENHDFLKLAINTVRNDIIGRNETFQCLALTLVGNIGGRDFAESLAPDVQKLLISSSCRPLVRKKAALCLLRLFRKNPDAVNVDGWADRMAQLLDERDLGVLTSSTSLLVALVSNNHEAYSSCLPKCVKILERLARNQDVPQEYTYYGIPSPWLQVKAMRALQYFPTIEDPSTRKALFEVLQRILMGTDVVKNVNKNNASHAVLFEALSLVMHLDAEKEMMSQCVALLGKFISVREPNIRYLGLENMTRMLMVTDVQDIIKKHQSQIITSLKDPDISIRRRALDLLYGMCDVSNAKDIVEELLQYLSTAEFSMREELSLKAAILAEKFAPDLSWYVDVILQLIDKAGDFVSDDIWFRVVQFVTNNEDLQPYAASKAREYMDKIAIHETMVKVSAYILGEYGHLLARQPGCSASELFSILHEKLPTVSTPTIPILLSTYAKLLMHAQPPDPELQKKVWAVFKKYESCIDVEIQQRAVEYFELSKKGPAFMDVLAEMPKFPERQSSLIKKAENVEDTADQSAIKLRAQQQPSNAIVLADPQPVNGAPPPLKVPILSGSTDPESVARSLSHPNGTLSNIDPQTPSPDLLSDLLGPLAIEAPPGAVSYEQHGPVGAEGVPDEIDGSAIVPVEEQTNTVELIGNIAERFHALCLKDSGVLYEDPHIQIGIKAEWRGHHGRLVLFMGNKNTSPLTSVQALILPPAHLRLDLSPVPDTIPPRAQVQSPLEVMNIRPSRDVAVLDFSYKFGTNVVSAKLRIPATLNKFLQPLQLTSEEFFPQWRAISGPPLKLQEVVRGVRPLALPEMANLFNSFHVTICPGLDPNPNNLVASTTFYSETTGAMLCLARIETDPADRTQLRLTVGSGDPTLTFELKEFIKEQLITIPMGSRALVPAAGPAPSPAVQPPSPAALADDPGAMLAGLL

Target ORF information:

RefSeq Version	NM_001343765.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Adaptor protein complex AP-2, alpha subunit (AT5G22780), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001343765.1

ORF Insert Sequence:

ATGACCGGAA TGAGAGGTCT CTCCGTATTT ATCAGCGACG TTCGTAATTG TCAGAACAAG 
GAAGCGGAGA GACTACGAGT TGATAAGGAG CTCGGAAACA TTCGTACATG CTTCAAGAAT 
GAGAAGGTCT TAACACCATA CAAAAAGAAG AAATATGTCT GGAAAATGCT TTATATACAT 
ATGCTAGGCT ACGATGTGGA TTTTGGCCAT ATGGAGGCAG TTTCTCTTAT ATCTGCGCCA 
AAGTATCCAG AAAAGCAGGT TGGCTACATT GTTACATCTT GCTTGCTCAA TGAGAACCAT 
GACTTTTTGA AGTTGGCTAT AAATACAGTG CGGAACGATA TTATTGGTCG GAATGAGACT 
TTCCAATGCT TAGCACTGAC CTTGGTTGGA AATATTGGTG GAAGAGATTT TGCTGAATCA 
TTGGCACCTG ATGTTCAAAA GCTACTTATT TCTAGTAGTT GCAGACCTCT CGTAAGGAAA 
AAAGCGGCAC TGTGCCTACT GCGCTTATTC CGAAAAAATC CGGATGCTGT CAACGTTGAT 
GGCTGGGCGG ATCGTATGGC ACAACTTTTG GATGAACGAG ATCTTGGTGT CTTGACGTCT 
TCTACGAGTC TTCTTGTAGC TCTGGTATCT AACAATCATG AGGCGTACTC AAGTTGTCTA 
CCTAAATGTG TCAAAATATT AGAACGCCTT GCTAGGAACC AGGATGTTCC TCAGGAGTAC 
ACATACTATG GCATCCCATC TCCTTGGCTC CAGGTTAAGG CAATGAGGGC TCTTCAATAT 
TTTCCAACCA TTGAAGATCC CAGTACCAGA AAAGCTCTCT TTGAGGTCCT TCAGCGAATA 
TTGATGGGCA CAGATGTTGT GAAAAATGTG AATAAAAACA ATGCGTCTCA TGCCGTGCTG 
TTTGAAGCTC TCTCCCTGGT CATGCATCTT GATGCTGAGA AGGAAATGAT GTCACAGTGT 
GTTGCTCTTC TTGGCAAGTT TATTTCTGTC CGTGAGCCCA ACATCAGATA TCTAGGCTTG 
GAAAATATGA CGCGAATGCT GATGGTCACA GATGTTCAAG ATATCATAAA GAAGCATCAG 
TCTCAGATAA TCACCTCGTT GAAAGACCCT GACATCAGTA TTCGTAGACG CGCTCTTGAT 
TTGCTATATG GGATGTGCGA TGTGTCAAAT GCAAAGGATA TAGTTGAAGA GTTGTTGCAG 
TATCTTAGCA CAGCAGAATT TTCTATGCGG GAGGAATTAT CACTTAAAGC TGCTATTCTT 
GCTGAGAAGT TTGCTCCTGA TCTTTCATGG TATGTAGATG TGATCCTTCA GTTGATCGAC 
AAGGCTGGGG ATTTTGTTAG CGATGACATT TGGTTTCGTG TGGTTCAGTT TGTAACAAAT 
AATGAAGACC TGCAGCCTTA TGCGGCATCA AAAGCAAGAG AATATATGGA CAAAATTGCA 
ATCCATGAGA CCATGGTGAA GGTCAGTGCT TATATTTTGG GGGAGTATGG TCACCTTTTA 
GCTAGACAAC CTGGATGCAG TGCAAGTGAA CTCTTTAGCA TCTTACATGA GAAGCTTCCT 
ACAGTATCGA CTCCCACCAT TCCTATTCTT CTTTCTACAT ATGCAAAGCT TCTTATGCAC 
GCTCAACCCC CGGATCCAGA ACTGCAAAAA AAAGTTTGGG CTGTATTTAA GAAATATGAG 
AGTTGTATCG ATGTGGAGAT ACAACAGAGG GCAGTTGAAT ATTTTGAATT GAGTAAAAAA 
GGGCCTGCCT TTATGGATGT ATTGGCTGAG ATGCCAAAAT TTCCGGAGCG CCAGTCTTCA 
TTGATTAAAA AGGCAGAAAA CGTTGAAGAT ACCGCAGACC AGAGTGCCAT CAAACTTAGA 
GCACAACAAC AGCCTTCAAA TGCTATTGTT TTGGCTGACC CACAGCCTGT TAATGGGGCA 
CCACCACCTT TGAAGGTTCC AATTTTAAGT GGAAGCACGG ACCCTGAGTC AGTGGCTCGA 
TCTCTCTCAC ATCCCAATGG AACTTTAAGT AATATAGATC CACAAACTCC ATCACCTGAT 
CTCCTTAGTG ACCTACTCGG TCCTCTTGCA ATAGAGGCTC CACCTGGAGC TGTCTCATAT 
GAACAACATG GTCCCGTAGG GGCAGAAGGA GTTCCAGATG AAATTGATGG TTCCGCCATT 
GTACCTGTTG AAGAGCAAAC AAATACAGTG GAGCTTATCG GAAATATTGC TGAGAGATTT 
CATGCTTTAT GTTTGAAAGA CAGTGGTGTC TTATACGAGG ATCCTCATAT CCAGATTGGC 
ATCAAAGCTG AATGGAGAGG ACACCATGGG AGACTTGTTC TCTTCATGGG AAACAAGAAT 
ACTTCTCCAC TTACATCAGT TCAAGCTCTG ATACTACCCC CTGCCCACTT AAGACTGGAT 
CTGTCTCCAG TACCTGATAC AATTCCTCCT CGAGCACAGG TACAATCTCC GCTTGAAGTG 
ATGAACATCC GCCCTAGTAG GGATGTTGCA GTTTTGGATT TCTCCTACAA GTTTGGTACA 
AACGTGGTCA GTGCCAAATT ACGCATCCCG GCAACCTTGA ATAAGTTTCT TCAACCTCTA 
CAATTGACAT CTGAAGAGTT CTTCCCTCAA TGGAGAGCAA TCTCAGGGCC ACCCTTGAAA 
CTTCAGGAAG TGGTAAGAGG TGTAAGACCT CTAGCTCTTC CGGAAATGGC AAATCTGTTC 
AACAGTTTTC ATGTGACGAT ATGCCCTGGG CTTGATCCAA ACCCTAATAA TCTAGTGGCA 
AGCACAACCT TCTACTCTGA AACTACAGGA GCCATGCTCT GTTTGGCAAG AATTGAAACA 
GACCCAGCGG ACAGAACCCA ACTACGATTG ACAGTAGGCT CGGGAGATCC TACCCTTACA 
TTTGAGTTAA AAGAATTCAT CAAGGAACAA TTAATTACTA TTCCAATGGG ATCTCGAGCT 
CTAGTTCCAG CAGCAGGTCC AGCACCATCA CCAGCAGTTC AACCGCCTAG CCCAGCGGCT 
TTAGCTGATG ATCCAGGAGC TATGCTTGCT GGCTTACTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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AT5G22780 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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