AP-2alpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	AP-2alpha
Entrez Gene ID	33211
Full Name	Adaptor Protein complex 2, alpha subunit
Synonyms	AP-2,AP2,Ada,Alpha-Adaptin,CG31654,CG4260,D-Ada,D-alphaAda,Dmel\CG4260,MENE (2L)-A,MENE(2L)-A,ada,alpha,alpha-Ada,alpha-Adaptin,alpha-ada,alpha-adaptin,alphaAda,apl3,cg4260,dAP-2a,l(2)06694,l(2)SH0460,l(2)SH2 0460
General protein information	Preferred Names Adaptor Protein complex 2, alpha subunit Names CG4260 gene product from transcript CG4260-RA AP-2alpha-PA AP-2alpha-PB Alpha-adaptin CG4260-PA CG4260-PB adaptor protein complex 2, alpha subunit alpha adaptin alphaAdaptin lethal (2) SH0460
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21C2-21C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057471.6	NP_476819.2	adaptor protein complex 2, alpha subunit, isoform A
NM_205885.2	NP_995607.1	adaptor protein complex 2, alpha subunit, isoform B

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	AP2A2	NP_001012914.1
Drosophila melanogaster (fruit fly)	alpha-Adaptin	NP_476819.2
Caenorhabditis elegans (roundworm)	apa-2	NP_509572.1
Neurospora crassa	NCU03440	XP_956726.1
Arabidopsis thaliana (thale cress)	alpha-ADR	NP_197669.1
Pan troglodytes (chimpanzee)	AP2A2	XP_003951843.1
Rattus norvegicus (Norway rat)	Ap2a2	NP_112270.2
Anopheles gambiae (African malaria mosquito)	AP2A_ANOGA	XP_310153.4
Arabidopsis thaliana (thale cress)	AT5G22780	NP_197670.1
Homo sapiens (human)	AP2A2	NP_001229766.1
	AP2A2	XP_001116794.2
Schizosaccharomyces pombe (fission yeast)	apl3	NP_595595.1
Magnaporthe oryzae (rice blast fungus)	MGG_09493	XP_003712251.1
Canis lupus familiaris (dog)	AP2A2	XP_005631788.1
Xenopus tropicalis (tropical clawed frog)	ap2a2	NP_001001209.1
Bos taurus (cattle)	AP2A2	NP_001069170.1
Mus musculus (house mouse)	Ap2a2	NP_031485.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The adaptor protein 2 (AP-2) complex binds to a motif in the cytoplasmic tail of Crumbs that overlaps with the binding site of Stardust, a protein known to stabilize Crumbs on the surface.
Title: AP-2-complex-mediated endocytosis of Drosophila Crumbs regulates polarity by antagonizing Stardust.

Interaction of Notch signaling modulator Numb with alpha-Adaptin regulates endocytosis of Notch pathway components and cell fate determination of neural stem cells.
Title: Interaction of Notch signaling modulator Numb with ?-Adaptin regulates endocytosis of Notch pathway components and cell fate determination of neural stem cells.

Numb and alpha-Adaptin regulate Sanpodo endocytosis to specify cell fate in Drosophila external sensory organs.
Title: Numb and alpha-Adaptin regulate Sanpodo endocytosis to specify cell fate in Drosophila external sensory organs.

Numb polarizes the distribution of alpha-Adaptin, involved in receptor-mediated endocytosis. Alpha-Adaptin mutations not binding to Numb don't localize asymmetrically and cause defects in asymmetric cell division.
Title: The endocytic protein alpha-Adaptin is required for numb-mediated asymmetric cell division in Drosophila.

The following AP-2alpha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AP-2alpha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13341	NM_057471.6 Latest version!	Drosophila melanogaster adaptor protein complex 2, alpha subunit, transcript variant A (AP-2alpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa13342	NM_205885.2 Latest version!	Drosophila melanogaster adaptor protein complex 2, alpha subunit, transcript variant B (AP-2alpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AP-2alpha ( NM_057471.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476819.2 Drosophila melanogaster adaptor protein complex 2, alpha subunit, transcript variant A (AP-2alpha), mRNA.

Price & Availability↑

CloneID	OFa13341
Clone ID Related Accession (Same CDS sequence)	NM_057471.6
Accession Version	NM_057471.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	adaptor protein complex 2, alpha subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057471.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCCGG TGCGGGGGGA TGGCATGAGA GGCCTGGCCG TCTTTATATC GGATATAAGG 
AACTGCAAAA GCAAAGAGGC CGAAGTCAAA CGCATAAACA AGGAGTTGGC CAATATACGA 
AGCAAATTTA AAGGAGACAA AACCCTCGAC GGCTATCAGA AAAAGAAATA TGTCTGTAAG 
CTGTTATTCA TATTTCTCCT CGGCCATGAC ATCGACTTCG GGCACATGGA GGCGGTCAAT 
TTGCTGTCCT CCAACAAATA CTCAGAAAAG CAGATTGGTT ACCTTTTCAT CTCAGTGTTG 
GTGAACACCA ACAGCGACCT TATTCGGCTG ATCATCCAAT CTATAAAGAA TGATTTGCAG 
TCGCGTAATC CTGTGCACGT TAATCTGGCA CTACAATGCA TTGCCAACAT TGGAAGTCGG 
GATATGGCCG AGTCCTTTTC CAACGAAATA CCCAAGCTAC TCGTCTCGGG CGACACCATG 
GACGTGGTGA AGCAGTCGGC GGCGCTATGC CTGCTCCGCC TGTTCCGCTC CTCGCCGGAC 
ATCATTCCCG GTGGTGAGTG GACTTCGCGC ATTATCCATT TGCTCAATGA TCAGCACATG 
GGAGTGGTCA CAGCGGCCAC CTCGCTGATC GACGCCTTGG TGAAGCGCAA TCCGGACGAG 
TACAAGGGTT GTGTTAATTT GGCTGTTTCA CGCTTATCCC GGATCGTAAC AGCCAGCTAC 
ACGGATCTTC AGGATTACAC ATATTACTTT GTGCCGGCTC CATGGCTATC GGTGAAACTT 
CTGCGATTGC TGCAAAACTA CAATCCAGTG ACCGAAGAGG CCGGTGTGCG GGCCCGCTTG 
AATGAAACTT TGGAGACGAT TTTGAACAAG GCGCAAGAGC CGCCAAAGAG TAAGAAGGTG 
CAGCACTCTA ACGCAAAGAA CGCCGTGCTC TTCGAGGCCA TCAACCTGAT CATTCACAGC 
GACAGCGAGC CGAACCTTCT TGTCCGCGCT TGCAACCAGT TGGGACAGTT CCTTAGCAAT 
CGGGAGACCA ACTTGCGCTA TCTGGCTCTG GAGTCCATGT GCCACCTGGC CACATCGGAG 
TTCTCGCACG AGGAGGTGAA GAAGCACCAG GAGGTGGTCA TTTTGTCAAT GAAAATGGAG 
AAGGACGTGT CCGTGCGCCA AATGGCGGTG GATCTTTTGT ATGCCATGTG TGATCGTGGT 
AATGCCGAGG AAATTGTTCA GGAGATGCTC AACTATCTGG AGACGGCCGA CTACTCTATT 
CGTGAGGAAA TGGTGCTCAA GGTGGCCATT CTTGCCGAGA AGTACGCCAC GGATTATACC 
TGGTATGTGG ACGTTATCCT CAATCTAATT CGCATTGCTG GCGACTACGT GTCTGAGGAA 
GTATGGTACC GCGTCATTCA GATCGTGATC AATCGCGAGG AGGTTCAAGG CTATGCCGCA 
AAGACTGTTT TTGAGGCGCT ACAGGCCCCT GCCTGCCACG AAAACATGGT TAAAGTGGGC 
GGCTACATCT TGGGCGAGTT TGGCAACTTA ATCGCCGGAG ATTCCCGCTC AGCACCACTG 
GTACAATTCA AGCTACTGCA CTCCAAGTAC CACTTGTGCT CGCCGATGAC CCGGGCCCTT 
CTTCTGTCCA CGTACATTAA ATTTATCAAC CTCTTTCCGG AAATTCGTAC CAATATCCAG 
GATGTGTTCC GCCAGCACAG CAATCTTCGA TCCGCAGATG CGGAACTGCA GCAGCGAGCT 
AGCGAATATC TCCAGCTTAG CATAGTGGCA TCGACAGACG TTTTGGCCAC CGTCCTGGAG 
GAGATGCCAT CGTTCCCGGA ACGCGAGAGC TCAATTTTAG CAGTGCTAAA GAAGAAGAAG 
CCCGGCAGGG TGCCGGAGAA CGAAATTCGC GAGTCGAAGA GCCCGGCACC CCTGACTTCT 
GCAGCGCAGA ACAACGCCCT TGTGAACAAT TCACATAGCA AGCTGAATAA CAGCAACGCA 
AATACGGATC TGCTTGGATT AAGCACTCCG CCATCAAATA ATATTGGCAG TGGTAGCAAT 
AGCAACAGTA CGCTCATTGA TGTTTTGGGC GACATGTACG GCAGCAATAG TAACAACAAC 
TCAAGTGCCG TGTACAACAC GAAGAAGTTT TTGTTCAAGA ACAATGGTGT TTTGTTTGAG 
AACGAAATGC TGCAGATCGG TGTGAAGAGC GAGTTTCGCC AGAATTTAGG ACGATTAGGC 
CTTTTCTACG GCAACAAGAC ACAGGTTCCC CTGACAAACT TCAATCCTGT GCTGCAATGG 
TCTGCCGAGG ATGCGCTTAA GCTGAACGTG CAGATGAAGG TGGTGGAGCC AACCCTGGAG 
GCCGGCGCCC AGATCCAACA ACTGTTAACC GCGGAATGCA TCGAGGACTA CGCCGATGCG 
CCAACAATCG AGATCAGTTT CCGCTACAAC GGCACCCAGC AGAAGTTCAG CATTAAACTG 
CCATTGAGCG TTAACAAATT CTTTGAGCCT ACCGAAATGA ATGCGGAATC ATTCTTTGCA 
CGATGGAAGA ACCTTAGCGG TGAACAACAA CGGTCACAGA AAGTGTTCAA GGCTGCACAG 
CCACTGGATT TGCCCGGAGC CCGCAACAAG CTTATGGGCT TTGGAATGCA ACTGCTGGAC 
CAAGTGGATC CCAATCCAGA CAACATGGTC TGCGCGGGCA TCATTCATAC GCAATCGCAA 
CAGGTGGGCT GCCTTATGCG ATTGGAGCCG AACAAGCAGG CTCAGATGTT CCGACTGACA 
GTTCGGGCAA GCAAGGAGAC CGTAACTCGG GAAATCTGCG ATCTGTTGAC GGATCAATTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476819.2
CDS	531..3353
Translation	MAPVRGDGMRGLAVFISDIRNCKSKEAEVKRINKELANIRSKFKGDKTLDGYQKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYSEKQIGYLFISVLVNTNSDLIRLIIQSIKNDLQSRNPVHVNLALQCIANIGSRDMAESFSNEIPKLLVSGDTMDVVKQSAALCLLRLFRSSPDIIPGGEWTSRIIHLLNDQHMGVVTAATSLIDALVKRNPDEYKGCVNLAVSRLSRIVTASYTDLQDYTYYFVPAPWLSVKLLRLLQNYNPVTEEAGVRARLNETLETILNKAQEPPKSKKVQHSNAKNAVLFEAINLIIHSDSEPNLLVRACNQLGQFLSNRETNLRYLALESMCHLATSEFSHEEVKKHQEVVILSMKMEKDVSVRQMAVDLLYAMCDRGNAEEIVQEMLNYLETADYSIREEMVLKVAILAEKYATDYTWYVDVILNLIRIAGDYVSEEVWYRVIQIVINREEVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDSRSAPLVQFKLLHSKYHLCSPMTRALLLSTYIKFINLFPEIRTNIQDVFRQHSNLRSADAELQQRASEYLQLSIVASTDVLATVLEEMPSFPERESSILAVLKKKKPGRVPENEIRESKSPAPLTSAAQNNALVNNSHSKLNNSNANTDLLGLSTPPSNNIGSGSNSNSTLIDVLGDMYGSNSNNNSSAVYNTKKFLFKNNGVLFENEMLQIGVKSEFRQNLGRLGLFYGNKTQVPLTNFNPVLQWSAEDALKLNVQMKVVEPTLEAGAQIQQLLTAECIEDYADAPTIEISFRYNGTQQKFSIKLPLSVNKFFEPTEMNAESFFARWKNLSGEQQRSQKVFKAAQPLDLPGARNKLMGFGMQLLDQVDPNPDNMVCAGIIHTQSQQVGCLMRLEPNKQAQMFRLTVRASKETVTREICDLLTDQF

Target ORF information:

RefSeq Version	NM_057471.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster adaptor protein complex 2, alpha subunit, transcript variant A (AP-2alpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057471.6

ORF Insert Sequence:

ATGGCGCCGG TGCGGGGGGA TGGCATGAGA GGCCTGGCCG TCTTTATATC GGATATAAGG 
AACTGCAAAA GCAAAGAGGC CGAAGTCAAA CGCATAAACA AGGAGTTGGC CAATATACGA 
AGCAAATTTA AAGGAGACAA AACCCTCGAC GGCTATCAGA AAAAGAAATA TGTCTGTAAG 
CTGTTATTCA TATTTCTCCT CGGCCATGAC ATCGACTTCG GGCACATGGA GGCGGTCAAT 
TTGCTGTCCT CCAACAAATA CTCAGAAAAG CAGATTGGTT ACCTTTTCAT CTCAGTGTTG 
GTGAACACCA ACAGCGACCT TATTCGGCTG ATCATCCAAT CTATAAAGAA TGATTTGCAG 
TCGCGTAATC CTGTGCACGT TAATCTGGCA CTACAATGCA TTGCCAACAT TGGAAGTCGG 
GATATGGCCG AGTCCTTTTC CAACGAAATA CCCAAGCTAC TCGTCTCGGG CGACACCATG 
GACGTGGTGA AGCAGTCGGC GGCGCTATGC CTGCTCCGCC TGTTCCGCTC CTCGCCGGAC 
ATCATTCCCG GTGGTGAGTG GACTTCGCGC ATTATCCATT TGCTCAATGA TCAGCACATG 
GGAGTGGTCA CAGCGGCCAC CTCGCTGATC GACGCCTTGG TGAAGCGCAA TCCGGACGAG 
TACAAGGGTT GTGTTAATTT GGCTGTTTCA CGCTTATCCC GGATCGTAAC AGCCAGCTAC 
ACGGATCTTC AGGATTACAC ATATTACTTT GTGCCGGCTC CATGGCTATC GGTGAAACTT 
CTGCGATTGC TGCAAAACTA CAATCCAGTG ACCGAAGAGG CCGGTGTGCG GGCCCGCTTG 
AATGAAACTT TGGAGACGAT TTTGAACAAG GCGCAAGAGC CGCCAAAGAG TAAGAAGGTG 
CAGCACTCTA ACGCAAAGAA CGCCGTGCTC TTCGAGGCCA TCAACCTGAT CATTCACAGC 
GACAGCGAGC CGAACCTTCT TGTCCGCGCT TGCAACCAGT TGGGACAGTT CCTTAGCAAT 
CGGGAGACCA ACTTGCGCTA TCTGGCTCTG GAGTCCATGT GCCACCTGGC CACATCGGAG 
TTCTCGCACG AGGAGGTGAA GAAGCACCAG GAGGTGGTCA TTTTGTCAAT GAAAATGGAG 
AAGGACGTGT CCGTGCGCCA AATGGCGGTG GATCTTTTGT ATGCCATGTG TGATCGTGGT 
AATGCCGAGG AAATTGTTCA GGAGATGCTC AACTATCTGG AGACGGCCGA CTACTCTATT 
CGTGAGGAAA TGGTGCTCAA GGTGGCCATT CTTGCCGAGA AGTACGCCAC GGATTATACC 
TGGTATGTGG ACGTTATCCT CAATCTAATT CGCATTGCTG GCGACTACGT GTCTGAGGAA 
GTATGGTACC GCGTCATTCA GATCGTGATC AATCGCGAGG AGGTTCAAGG CTATGCCGCA 
AAGACTGTTT TTGAGGCGCT ACAGGCCCCT GCCTGCCACG AAAACATGGT TAAAGTGGGC 
GGCTACATCT TGGGCGAGTT TGGCAACTTA ATCGCCGGAG ATTCCCGCTC AGCACCACTG 
GTACAATTCA AGCTACTGCA CTCCAAGTAC CACTTGTGCT CGCCGATGAC CCGGGCCCTT 
CTTCTGTCCA CGTACATTAA ATTTATCAAC CTCTTTCCGG AAATTCGTAC CAATATCCAG 
GATGTGTTCC GCCAGCACAG CAATCTTCGA TCCGCAGATG CGGAACTGCA GCAGCGAGCT 
AGCGAATATC TCCAGCTTAG CATAGTGGCA TCGACAGACG TTTTGGCCAC CGTCCTGGAG 
GAGATGCCAT CGTTCCCGGA ACGCGAGAGC TCAATTTTAG CAGTGCTAAA GAAGAAGAAG 
CCCGGCAGGG TGCCGGAGAA CGAAATTCGC GAGTCGAAGA GCCCGGCACC CCTGACTTCT 
GCAGCGCAGA ACAACGCCCT TGTGAACAAT TCACATAGCA AGCTGAATAA CAGCAACGCA 
AATACGGATC TGCTTGGATT AAGCACTCCG CCATCAAATA ATATTGGCAG TGGTAGCAAT 
AGCAACAGTA CGCTCATTGA TGTTTTGGGC GACATGTACG GCAGCAATAG TAACAACAAC 
TCAAGTGCCG TGTACAACAC GAAGAAGTTT TTGTTCAAGA ACAATGGTGT TTTGTTTGAG 
AACGAAATGC TGCAGATCGG TGTGAAGAGC GAGTTTCGCC AGAATTTAGG ACGATTAGGC 
CTTTTCTACG GCAACAAGAC ACAGGTTCCC CTGACAAACT TCAATCCTGT GCTGCAATGG 
TCTGCCGAGG ATGCGCTTAA GCTGAACGTG CAGATGAAGG TGGTGGAGCC AACCCTGGAG 
GCCGGCGCCC AGATCCAACA ACTGTTAACC GCGGAATGCA TCGAGGACTA CGCCGATGCG 
CCAACAATCG AGATCAGTTT CCGCTACAAC GGCACCCAGC AGAAGTTCAG CATTAAACTG 
CCATTGAGCG TTAACAAATT CTTTGAGCCT ACCGAAATGA ATGCGGAATC ATTCTTTGCA 
CGATGGAAGA ACCTTAGCGG TGAACAACAA CGGTCACAGA AAGTGTTCAA GGCTGCACAG 
CCACTGGATT TGCCCGGAGC CCGCAACAAG CTTATGGGCT TTGGAATGCA ACTGCTGGAC 
CAAGTGGATC CCAATCCAGA CAACATGGTC TGCGCGGGCA TCATTCATAC GCAATCGCAA 
CAGGTGGGCT GCCTTATGCG ATTGGAGCCG AACAAGCAGG CTCAGATGTT CCGACTGACA 
GTTCGGGCAA GCAAGGAGAC CGTAACTCGG GAAATCTGCG ATCTGTTGAC GGATCAATTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP-2alpha ( NM_205885.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995607.1 Drosophila melanogaster adaptor protein complex 2, alpha subunit, transcript variant B (AP-2alpha), mRNA.

Price & Availability↑

CloneID	OFa13342
Clone ID Related Accession (Same CDS sequence)	NM_205885.2
Accession Version	NM_205885.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	adaptor protein complex 2, alpha subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On May 8, 2012 this sequence version replaced NM_205885.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAGAG CTGAAGGGTG TGAAGTCGTC TCTCCGATCG ATCAATCGCG GGGTGGAAAG 
GAAAGCGAAG CTTCCGCAGA CGAATCACTG TTGTTTTACT GCAAAAGCAA AGAGGCCGAA 
GTCAAACGCA TAAACAAGGA GTTGGCCAAT ATACGAAGCA AATTTAAAGG AGACAAAACC 
CTCGACGGCT ATCAGAAAAA GAAATATGTC TGTAAGCTGT TATTCATATT TCTCCTCGGC 
CATGACATCG ACTTCGGGCA CATGGAGGCG GTCAATTTGC TGTCCTCCAA CAAATACTCA 
GAAAAGCAGA TTGGTTACCT TTTCATCTCA GTGTTGGTGA ACACCAACAG CGACCTTATT 
CGGCTGATCA TCCAATCTAT AAAGAATGAT TTGCAGTCGC GTAATCCTGT GCACGTTAAT 
CTGGCACTAC AATGCATTGC CAACATTGGA AGTCGGGATA TGGCCGAGTC CTTTTCCAAC 
GAAATACCCA AGCTACTCGT CTCGGGCGAC ACCATGGACG TGGTGAAGCA GTCGGCGGCG 
CTATGCCTGC TCCGCCTGTT CCGCTCCTCG CCGGACATCA TTCCCGGTGG TGAGTGGACT 
TCGCGCATTA TCCATTTGCT CAATGATCAG CACATGGGAG TGGTCACAGC GGCCACCTCG 
CTGATCGACG CCTTGGTGAA GCGCAATCCG GACGAGTACA AGGGTTGTGT TAATTTGGCT 
GTTTCACGCT TATCCCGGAT CGTAACAGCC AGCTACACGG ATCTTCAGGA TTACACATAT 
TACTTTGTGC CGGCTCCATG GCTATCGGTG AAACTTCTGC GATTGCTGCA AAACTACAAT 
CCAGTGACCG AAGAGGCCGG TGTGCGGGCC CGCTTGAATG AAACTTTGGA GACGATTTTG 
AACAAGGCGC AAGAGCCGCC AAAGAGTAAG AAGGTGCAGC ACTCTAACGC AAAGAACGCC 
GTGCTCTTCG AGGCCATCAA CCTGATCATT CACAGCGACA GCGAGCCGAA CCTTCTTGTC 
CGCGCTTGCA ACCAGTTGGG ACAGTTCCTT AGCAATCGGG AGACCAACTT GCGCTATCTG 
GCTCTGGAGT CCATGTGCCA CCTGGCCACA TCGGAGTTCT CGCACGAGGA GGTGAAGAAG 
CACCAGGAGG TGGTCATTTT GTCAATGAAA ATGGAGAAGG ACGTGTCCGT GCGCCAAATG 
GCGGTGGATC TTTTGTATGC CATGTGTGAT CGTGGTAATG CCGAGGAAAT TGTTCAGGAG 
ATGCTCAACT ATCTGGAGAC GGCCGACTAC TCTATTCGTG AGGAAATGGT GCTCAAGGTG 
GCCATTCTTG CCGAGAAGTA CGCCACGGAT TATACCTGGT ATGTGGACGT TATCCTCAAT 
CTAATTCGCA TTGCTGGCGA CTACGTGTCT GAGGAAGTAT GGTACCGCGT CATTCAGATC 
GTGATCAATC GCGAGGAGGT TCAAGGCTAT GCCGCAAAGA CTGTTTTTGA GGCGCTACAG 
GCCCCTGCCT GCCACGAAAA CATGGTTAAA GTGGGCGGCT ACATCTTGGG CGAGTTTGGC 
AACTTAATCG CCGGAGATTC CCGCTCAGCA CCACTGGTAC AATTCAAGCT ACTGCACTCC 
AAGTACCACT TGTGCTCGCC GATGACCCGG GCCCTTCTTC TGTCCACGTA CATTAAATTT 
ATCAACCTCT TTCCGGAAAT TCGTACCAAT ATCCAGGATG TGTTCCGCCA GCACAGCAAT 
CTTCGATCCG CAGATGCGGA ACTGCAGCAG CGAGCTAGCG AATATCTCCA GCTTAGCATA 
GTGGCATCGA CAGACGTTTT GGCCACCGTC CTGGAGGAGA TGCCATCGTT CCCGGAACGC 
GAGAGCTCAA TTTTAGCAGT GCTAAAGAAG AAGAAGCCCG GCAGGGTGCC GGAGAACGAA 
ATTCGCGAGT CGAAGAGCCC GGCACCCCTG ACTTCTGCAG CGCAGAACAA CGCCCTTGTG 
AACAATTCAC ATAGCAAGCT GAATAACAGC AACGCAAATA CGGATCTGCT TGGATTAAGC 
ACTCCGCCAT CAAATAATAT TGGCAGTGGT AGCAATAGCA ACAGTACGCT CATTGATGTT 
TTGGGCGACA TGTACGGCAG CAATAGTAAC AACAACTCAA GTGCCGTGTA CAACACGAAG 
AAGTTTTTGT TCAAGAACAA TGGTGTTTTG TTTGAGAACG AAATGCTGCA GATCGGTGTG 
AAGAGCGAGT TTCGCCAGAA TTTAGGACGA TTAGGCCTTT TCTACGGCAA CAAGACACAG 
GTTCCCCTGA CAAACTTCAA TCCTGTGCTG CAATGGTCTG CCGAGGATGC GCTTAAGCTG 
AACGTGCAGA TGAAGGTGGT GGAGCCAACC CTGGAGGCCG GCGCCCAGAT CCAACAACTG 
TTAACCGCGG AATGCATCGA GGACTACGCC GATGCGCCAA CAATCGAGAT CAGTTTCCGC 
TACAACGGCA CCCAGCAGAA GTTCAGCATT AAACTGCCAT TGAGCGTTAA CAAATTCTTT 
GAGCCTACCG AAATGAATGC GGAATCATTC TTTGCACGAT GGAAGAACCT TAGCGGTGAA 
CAACAACGGT CACAGAAAGT GTTCAAGGCT GCACAGCCAC TGGATTTGCC CGGAGCCCGC 
AACAAGCTTA TGGGCTTTGG AATGCAACTG CTGGACCAAG TGGATCCCAA TCCAGACAAC 
ATGGTCTGCG CGGGCATCAT TCATACGCAA TCGCAACAGG TGGGCTGCCT TATGCGATTG 
GAGCCGAACA AGCAGGCTCA GATGTTCCGA CTGACAGTTC GGGCAAGCAA GGAGACCGTA 
ACTCGGGAAA TCTGCGATCT GTTGACGGAT CAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995607.1
CDS	62..2920
Translation	MERAEGCEVVSPIDQSRGGKESEASADESLLFYCKSKEAEVKRINKELANIRSKFKGDKTLDGYQKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYSEKQIGYLFISVLVNTNSDLIRLIIQSIKNDLQSRNPVHVNLALQCIANIGSRDMAESFSNEIPKLLVSGDTMDVVKQSAALCLLRLFRSSPDIIPGGEWTSRIIHLLNDQHMGVVTAATSLIDALVKRNPDEYKGCVNLAVSRLSRIVTASYTDLQDYTYYFVPAPWLSVKLLRLLQNYNPVTEEAGVRARLNETLETILNKAQEPPKSKKVQHSNAKNAVLFEAINLIIHSDSEPNLLVRACNQLGQFLSNRETNLRYLALESMCHLATSEFSHEEVKKHQEVVILSMKMEKDVSVRQMAVDLLYAMCDRGNAEEIVQEMLNYLETADYSIREEMVLKVAILAEKYATDYTWYVDVILNLIRIAGDYVSEEVWYRVIQIVINREEVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDSRSAPLVQFKLLHSKYHLCSPMTRALLLSTYIKFINLFPEIRTNIQDVFRQHSNLRSADAELQQRASEYLQLSIVASTDVLATVLEEMPSFPERESSILAVLKKKKPGRVPENEIRESKSPAPLTSAAQNNALVNNSHSKLNNSNANTDLLGLSTPPSNNIGSGSNSNSTLIDVLGDMYGSNSNNNSSAVYNTKKFLFKNNGVLFENEMLQIGVKSEFRQNLGRLGLFYGNKTQVPLTNFNPVLQWSAEDALKLNVQMKVVEPTLEAGAQIQQLLTAECIEDYADAPTIEISFRYNGTQQKFSIKLPLSVNKFFEPTEMNAESFFARWKNLSGEQQRSQKVFKAAQPLDLPGARNKLMGFGMQLLDQVDPNPDNMVCAGIIHTQSQQVGCLMRLEPNKQAQMFRLTVRASKETVTREICDLLTDQF

Target ORF information:

RefSeq Version	NM_205885.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster adaptor protein complex 2, alpha subunit, transcript variant B (AP-2alpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205885.2

ORF Insert Sequence:

ATGGAGAGAG CTGAAGGGTG TGAAGTCGTC TCTCCGATCG ATCAATCGCG GGGTGGAAAG 
GAAAGCGAAG CTTCCGCAGA CGAATCACTG TTGTTTTACT GCAAAAGCAA AGAGGCCGAA 
GTCAAACGCA TAAACAAGGA GTTGGCCAAT ATACGAAGCA AATTTAAAGG AGACAAAACC 
CTCGACGGCT ATCAGAAAAA GAAATATGTC TGTAAGCTGT TATTCATATT TCTCCTCGGC 
CATGACATCG ACTTCGGGCA CATGGAGGCG GTCAATTTGC TGTCCTCCAA CAAATACTCA 
GAAAAGCAGA TTGGTTACCT TTTCATCTCA GTGTTGGTGA ACACCAACAG CGACCTTATT 
CGGCTGATCA TCCAATCTAT AAAGAATGAT TTGCAGTCGC GTAATCCTGT GCACGTTAAT 
CTGGCACTAC AATGCATTGC CAACATTGGA AGTCGGGATA TGGCCGAGTC CTTTTCCAAC 
GAAATACCCA AGCTACTCGT CTCGGGCGAC ACCATGGACG TGGTGAAGCA GTCGGCGGCG 
CTATGCCTGC TCCGCCTGTT CCGCTCCTCG CCGGACATCA TTCCCGGTGG TGAGTGGACT 
TCGCGCATTA TCCATTTGCT CAATGATCAG CACATGGGAG TGGTCACAGC GGCCACCTCG 
CTGATCGACG CCTTGGTGAA GCGCAATCCG GACGAGTACA AGGGTTGTGT TAATTTGGCT 
GTTTCACGCT TATCCCGGAT CGTAACAGCC AGCTACACGG ATCTTCAGGA TTACACATAT 
TACTTTGTGC CGGCTCCATG GCTATCGGTG AAACTTCTGC GATTGCTGCA AAACTACAAT 
CCAGTGACCG AAGAGGCCGG TGTGCGGGCC CGCTTGAATG AAACTTTGGA GACGATTTTG 
AACAAGGCGC AAGAGCCGCC AAAGAGTAAG AAGGTGCAGC ACTCTAACGC AAAGAACGCC 
GTGCTCTTCG AGGCCATCAA CCTGATCATT CACAGCGACA GCGAGCCGAA CCTTCTTGTC 
CGCGCTTGCA ACCAGTTGGG ACAGTTCCTT AGCAATCGGG AGACCAACTT GCGCTATCTG 
GCTCTGGAGT CCATGTGCCA CCTGGCCACA TCGGAGTTCT CGCACGAGGA GGTGAAGAAG 
CACCAGGAGG TGGTCATTTT GTCAATGAAA ATGGAGAAGG ACGTGTCCGT GCGCCAAATG 
GCGGTGGATC TTTTGTATGC CATGTGTGAT CGTGGTAATG CCGAGGAAAT TGTTCAGGAG 
ATGCTCAACT ATCTGGAGAC GGCCGACTAC TCTATTCGTG AGGAAATGGT GCTCAAGGTG 
GCCATTCTTG CCGAGAAGTA CGCCACGGAT TATACCTGGT ATGTGGACGT TATCCTCAAT 
CTAATTCGCA TTGCTGGCGA CTACGTGTCT GAGGAAGTAT GGTACCGCGT CATTCAGATC 
GTGATCAATC GCGAGGAGGT TCAAGGCTAT GCCGCAAAGA CTGTTTTTGA GGCGCTACAG 
GCCCCTGCCT GCCACGAAAA CATGGTTAAA GTGGGCGGCT ACATCTTGGG CGAGTTTGGC 
AACTTAATCG CCGGAGATTC CCGCTCAGCA CCACTGGTAC AATTCAAGCT ACTGCACTCC 
AAGTACCACT TGTGCTCGCC GATGACCCGG GCCCTTCTTC TGTCCACGTA CATTAAATTT 
ATCAACCTCT TTCCGGAAAT TCGTACCAAT ATCCAGGATG TGTTCCGCCA GCACAGCAAT 
CTTCGATCCG CAGATGCGGA ACTGCAGCAG CGAGCTAGCG AATATCTCCA GCTTAGCATA 
GTGGCATCGA CAGACGTTTT GGCCACCGTC CTGGAGGAGA TGCCATCGTT CCCGGAACGC 
GAGAGCTCAA TTTTAGCAGT GCTAAAGAAG AAGAAGCCCG GCAGGGTGCC GGAGAACGAA 
ATTCGCGAGT CGAAGAGCCC GGCACCCCTG ACTTCTGCAG CGCAGAACAA CGCCCTTGTG 
AACAATTCAC ATAGCAAGCT GAATAACAGC AACGCAAATA CGGATCTGCT TGGATTAAGC 
ACTCCGCCAT CAAATAATAT TGGCAGTGGT AGCAATAGCA ACAGTACGCT CATTGATGTT 
TTGGGCGACA TGTACGGCAG CAATAGTAAC AACAACTCAA GTGCCGTGTA CAACACGAAG 
AAGTTTTTGT TCAAGAACAA TGGTGTTTTG TTTGAGAACG AAATGCTGCA GATCGGTGTG 
AAGAGCGAGT TTCGCCAGAA TTTAGGACGA TTAGGCCTTT TCTACGGCAA CAAGACACAG 
GTTCCCCTGA CAAACTTCAA TCCTGTGCTG CAATGGTCTG CCGAGGATGC GCTTAAGCTG 
AACGTGCAGA TGAAGGTGGT GGAGCCAACC CTGGAGGCCG GCGCCCAGAT CCAACAACTG 
TTAACCGCGG AATGCATCGA GGACTACGCC GATGCGCCAA CAATCGAGAT CAGTTTCCGC 
TACAACGGCA CCCAGCAGAA GTTCAGCATT AAACTGCCAT TGAGCGTTAA CAAATTCTTT 
GAGCCTACCG AAATGAATGC GGAATCATTC TTTGCACGAT GGAAGAACCT TAGCGGTGAA 
CAACAACGGT CACAGAAAGT GTTCAAGGCT GCACAGCCAC TGGATTTGCC CGGAGCCCGC 
AACAAGCTTA TGGGCTTTGG AATGCAACTG CTGGACCAAG TGGATCCCAA TCCAGACAAC 
ATGGTCTGCG CGGGCATCAT TCATACGCAA TCGCAACAGG TGGGCTGCCT TATGCGATTG 
GAGCCGAACA AGCAGGCTCA GATGTTCCGA CTGACAGTTC GGGCAAGCAA GGAGACCGTA 
ACTCGGGAAA TCTGCGATCT GTTGACGGAT CAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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AP-2alpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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