AP2A2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AP2A2
Entrez Gene ID	161
Full Name	adaptor related protein complex 2 alpha 2 subunit
Synonyms	ADTAB,CLAPA2,HIP-9,HIP9,HYPJ
General protein information	Preferred Names adaptor related protein complex 2 alpha 2 subunit Names AP-2 complex subunit alpha-2 100 kDa coated vesicle protein C adapter-related protein complex 2 subunit alpha-2 adaptin, alpha B adaptor-related protein complex 2 subunit alpha-2 alpha-adaptin C; Huntingtin interacting protein J alpha2-adaptin clathrin assembly protein complex 2 alpha-C large chain clathrin-associated/assembly/adaptor protein, large, alpha 2 huntingtin yeast partner J huntingtin-interacting protein 9 plasma membrane adaptor HA2/AP2 adaptin alpha C subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.5
Summary	The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane. [provided by RefSeq, Nov 2016].
Disorder MIM:	607242

mRNA and Protein(s)

mRNA	Protein	Name
XM_011519929.1	XP_011518231.1	AP-2 complex subunit alpha-2 isoform X1
NM_012305.3	NP_036437.1	AP-2 complex subunit alpha-2 isoform 2
XM_011519930.3	XP_011518232.1	AP-2 complex subunit alpha-2 isoform X2
XM_011519930.2	XP_011518232.1	AP-2 complex subunit alpha-2 isoform X2
NM_001242837.1	NP_001229766.1	AP-2 complex subunit alpha-2 isoform 1
NM_012305.4	NP_036437.1	AP-2 complex subunit alpha-2 isoform 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	AP2A2	NP_001012914.1
Drosophila melanogaster (fruit fly)	alpha-Adaptin	NP_476819.2
Caenorhabditis elegans (roundworm)	apa-2	NP_509572.1
Neurospora crassa	NCU03440	XP_956726.1
Arabidopsis thaliana (thale cress)	alpha-ADR	NP_197669.1
Pan troglodytes (chimpanzee)	AP2A2	XP_003951843.1
Rattus norvegicus (Norway rat)	Ap2a2	NP_112270.2
Anopheles gambiae (African malaria mosquito)	AP2A_ANOGA	XP_310153.4
Arabidopsis thaliana (thale cress)	AT5G22780	NP_197670.1
Homo sapiens (human)	AP2A2	NP_001229766.1
	AP2A2	XP_001116794.2
Schizosaccharomyces pombe (fission yeast)	apl3	NP_595595.1
Magnaporthe oryzae (rice blast fungus)	MGG_09493	XP_003712251.1
Canis lupus familiaris (dog)	AP2A2	XP_005631788.1
Xenopus tropicalis (tropical clawed frog)	ap2a2	NP_001001209.1
Bos taurus (cattle)	AP2A2	NP_001069170.1
Mus musculus (house mouse)	Ap2a2	NP_031485.3

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of clathrin-mediated endocytosis by hierarchical allosteric activation of AP2.
Kadlecova Z, Spielman SJ, Loerke D, Mohanakrishnan A, Reed DK, Schmid SL
The Journal of cell biology216(1)167-179(2017 Jan)

CALM regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature.
Miller SE, Mathiasen S, Bright NA, Pierre F, Kelly BT, Kladt N, Schauss A, Merrifield CJ, Stamou D, H?ning S, Owen DJ
Developmental cell33(2)163-75(2015 Apr)

Genetic susceptibility for chronic bronchitis in chronic obstructive pulmonary disease.
Lee JH, Cho MH, Hersh CP, McDonald ML, Crapo JD, Bakke PS, Gulsvik A, Comellas AP, Wendt CH, Lomas DA, Kim V, Silverman EK,
Respiratory research15113(2014 Sep)

Meta-analysis of 74,046 individuals identifies 11 new susceptibility loci for Alzheimer's disease.
Lambert JC, Ibrahim-Verbaas CA, Harold D, Naj AC, Sims R, Bellenguez C, DeStafano AL, Bis JC, Beecham GW, Grenier-Boley B, Russo G, Thorton-Wells TA, Jones N, Smith AV, Chouraki V, Thomas C, Ikram MA, Zelenika D, Vardarajan BN, Kamatani Y, Lin CF, Gerrish A, Schmidt H, Kunkle B, Dunstan ML, Ruiz A, Bihoreau MT, Choi SH, Reitz C, Pasquier F, Cruchaga C, Craig D, Amin N, Berr C, Lopez OL, De Jager PL, Deramecourt V, Johnston JA, Evans D, Lovestone S, Letenneur L, Mor?n FJ, Rubinsztein DC, Eiriksdottir G, Sleegers K, Goate AM, Fi?vet N, Huentelman MW, Gill M, Brown K, Kamboh MI, Keller L, Barberger-Gateau P, McGuiness B, Larson EB, Green R, Myers AJ, Dufouil C, Todd S, Wallon D, Love S, Rogaeva E, Gallacher J, St George-Hyslop P, Clarimon J, Lleo A, Bayer A, Tsuang DW, Yu L, Tsolaki M, Boss? P, Spalletta G, Proitsi P, Collinge J, Sorbi S, Sanchez-Garcia F, Fox NC, Hardy J, Deniz Naranjo MC, Bosco P, Clarke R, Brayne C, Galimberti D, Mancuso M, Matthews F, , , , , Moebus S, Mecocci P, Del Zompo M, Maier W, Hampel H, Pilotto A, Bullido M, Panza F, Caffarra P, Nacmias B, Gilbert JR, Mayhaus M, Lannefelt L, Hakonarson H, Pichler S, Carrasquillo MM, Ingelsson M, Beekly D, Alvarez V, Zou F, Valladares O, Younkin SG, Coto E, Hamilton-Nelson KL, Gu W, Razquin C, Pastor P, Mateo I, Owen MJ, Faber KM, Jonsson PV, Combarros O, O'Donovan MC, Cantwell LB, Soininen H, Blacker D, Mead S, Mosley TH Jr, Bennett DA, Harris TB, Fratiglioni L, Holmes C, de Bruijn RF, Passmore P, Montine TJ, Bettens K, Rotter JI, Brice A, Morgan K, Foroud TM, Kukull WA, Hannequin D, Powell JF, Nalls MA, Ritchie K, Lunetta KL, Kauwe JS, Boerwinkle E, Riemenschneider M, Boada M, Hiltuenen M, Martin ER, Schmidt R, Rujescu D, Wang LS, Dartigues JF, Mayeux R, Tzourio C, Hofman A, N?then MM, Graff C, Psaty BM, Jones L, Haines JL, Holmans PA, Lathrop M, Pericak-Vance MA, Launer LJ, Farrer LA, van Duijn CM, Van Broeckhoven C, Moskvina V, Seshadri S, Williams J, Schellenberg GD, Amouyel P
Nature genetics45(12)1452-8(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AP2 has evolved as a key regulatory node to coordinate clarhtin-coated pit formation and cargo sorting and ensure high spatial and temporal regulation of cathrin-mediated endocytosis.
Title: Regulation of clathrin-mediated endocytosis by hierarchical allosteric activation of AP2.

A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration.
Title: Proteomic analysis identifies dysfunction in cellular transport, energy, and protein metabolism in different brain regions of atypical frontotemporal lobar degeneration.

Clathrin-dependent endocytosis of human CD317 from the cell surface lipid rafts is mediated by direct interaction with alpha-adaptin.
Title: HM1.24 is internalized from lipid rafts by clathrin-mediated endocytosis through interaction with alpha-adaptin.

The mutation of lysine-297 and arginine-340 on the alpha-adaptin specifically inhibits the ability of Nef to bind AP-2 and downregulate CD4.
Title: A basic patch on alpha-adaptin is required for binding of human immunodeficiency virus type 1 Nef and cooperative assembly of a CD4-Nef-AP-2 complex.

the AP-2 complex is not associated with the endocytosed plasma membrane-associated clathrin
Title: The AP-2 complex is excluded from the dynamic population of plasma membrane-associated clathrin.

The following AP2A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AP2A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21793	NM_012305.4 Latest version!	Homo sapiens adaptor related protein complex 2 subunit alpha 2 (AP2A2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu56208	XM_011519930.3 Latest version!	Homo sapiens adaptor related protein complex 2 subunit alpha 2 (AP2A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu22502	NM_001242837.1 Latest version!	Homo sapiens adaptor related protein complex 2 subunit alpha 2 (AP2A2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu56207	XM_011519929.1 Latest version!	Homo sapiens adaptor related protein complex 2 subunit alpha 2 (AP2A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu56208	XM_011519930.2	Homo sapiens adaptor related protein complex 2 alpha 2 subunit (AP2A2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu21793	NM_012305.3	Homo sapiens adaptor related protein complex 2 subunit alpha 2 (AP2A2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AP2A2 ( NM_012305.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036437.1 Homo sapiens adaptor related protein complex 2 subunit alpha 2 (AP2A2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21793
Clone ID Related Accession (Same CDS sequence)	NM_012305.3 , NM_012305.4
Accession Version	NM_012305.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	AP-2 complex subunit alpha-2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB461570.1, BC006155.1, AP006477.2 and BU735076.1. On Jun 30, 2011 this sequence version replaced NM_012305.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB020706.2, BC006155.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGCCG TGTCCAAGGG GGACGGGATG CGGGGCCTGG CGGTCTTCAT CTCGGATATC 
CGCAACTGTA AAAGTAAAGA AGCAGAAATA AAAAGGATAA ACAAGGAACT GGCAAATATC 
AGATCAAAAT TTAAAGGTGA CAAGGCTCTT GATGGCTATA GTAAAAAAAA GTACGTCTGC 
AAGTTGCTCT TCATCTTTCT CCTTGGTCAT GACATTGACT TTGGACACAT GGAGGCTGTG 
AACCTGCTGA GTTCAAACAG ATACACGGAA AAGCAGATCG GCTACCTTTT CATCTCTGTG 
TTGGTGAACT CAAACAGTGA GCTGATCCGC CTGATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGGGCCTG GCCCTGCACT GCATCGCCAG CGTGGGCAGC 
CGGGAGATGG CCGAGGCCTT CGCCGGGGAG ATCCCTAAGG TCCTCGTAGC CGGAGACACT 
ATGGACAGCG TGAAGCAGAG CGCGGCCCTG TGCTTGCTGC GCCTGTACAG GACGTCCCCC 
GATCTTGTCC CCATGGGCGA CTGGACATCC CGAGTGGTGC ACCTGCTCAA TGACCAGCAC 
TTGGGTGTGG TAACTGCAGC CACAAGTCTG ATCACCACTT TAGCACAGAA GAACCCAGAA 
GAGTTTAAAA CCTCCGTGTC TCTGGCTGTC TCTAGGCTAA GCAGAATCGT GACGTCTGCA 
TCCACAGATC TCCAGGATTA CACTTACTAT TTTGTCCCGG CTCCCTGGCT GTCTGTCAAA 
CTGCTGAGAC TGCTGCAGTG CTACCCACCC CCAGACCCTG CAGTGCGAGG CCGCCTGACT 
GAGTGCCTGG AGACCATCCT GAACAAAGCC CAAGAACCGC CCAAGTCGAA GAAGGTCCAG 
CACTCCAACG CGAAGAATGC CGTGCTCTTC GAGGCCATCA GCTTAATCAT TCACCATGAC 
AGTGAGCCGA ACCTGCTCGT CCGTGCCTGC AACCAGTTGG GCCAGTTTCT GCAGCACCGC 
GAGACCAACC TGCGCTACCT GGCCCTGGAG AGCATGTGCA CGCTGGCCAG CTCTGAGTTC 
TCCCATGAGG CTGTCAAGAC GCACATCGAG ACGGTCATCA ACGCCCTGAA GACTGAGCGG 
GACGTGAGCG TGCGGCAGCG GGCCGTGGAC CTCCTCTACG CCATGTGCGA CCGCAGCAAC 
GCCCCACAGA TCGTGGCCGA GATGCTGAGC TATCTGGAGA CAGCTGACTA CTCCATCCGA 
GAAGAGATTG TGCTGAAGGT CGCCATCCTG GCTGAGAAGT ACGCGGTGGA CTACACCTGG 
TATGTGGATA CCATCTTGAA CTTGATCCGA ATTGCTGGTG ATTACGTGAG TGAAGAGGTG 
TGGTACCGAG TCATTCAGAT CGTCATCAAC CGGGACGACG TGCAGGGCTA CGCGGCCAAG 
ACTGTGTTCG AGGCTCTTCA GGCTCCCGCG TGCCACGAGA ACCTGGTCAA AGTGGGCGGC 
TACATCCTGG GGGAGTTTGG AAACTTGATA GCTGGAGACC CGAGATCCAG CCCGCTGATC 
CAGTTCCACC TGCTGCACTC CAAGTTCCAC CTGTGCAGCG TCCCCACCCG CGCGCTGCTC 
CTGTCCACCT ACATCAAGTT CGTGAACCTC TTCCCGGAGG TGAAGCCCAC CATCCAGGAC 
GTGCTGCGCA GCGACAGCCA GCTCAGGAAC GCAGACGTGG AGCTGCAGCA GCGTGCTGTG 
GAGTACCTGC GGCTCAGCAC CGTGGCCAGC ACCGACATTC TGGCGACCGT GCTGGAGGAG 
ATGCCCCCAT TCCCGGAGCG GGAGTCCTCC ATCTTGGCAA AGCTCAAGAA GAAGAAGGGC 
CCCAGCACGG TGACAGACCT GGAGGACACC AAGCGGGACA GGAGTGTGGA CGTGAACGGG 
GGTCCTGAGC CTGCCCCAGC CAGTACCAGC GCCGTGTCTA CGCCTTCTCC GTCGGCAGAC 
CTGCTGGGTC TCGGGGCTGC CCCCCCTGCC CCCGCGGGCC CCCCACCCTC CTCCGGCGGC 
AGCGGGCTGC TCGTGGACGT GTTCTCAGAC TCGGCCTCTG TGGTCGCGCC TCTCGCTCCT 
GGCTCCGAAG ACAACTTTGC CAGGTTTGTT TGTAAAAACA ATGGTGTGTT GTTTGAAAAC 
CAGCTGCTTC AAATTGGACT TAAGTCTGAA TTTCGGCAGA ATTTAGGTCG GATGTTTATC 
TTTTATGGTA ATAAGACCTC CACGCAGTTC CTAAACTTTA CCCCAACACT AATCTGTTCA 
GACGACCTTC AGCCTAACCT GAACCTGCAG ACCAAGCCCG TGGACCCGAC CGTGGAGGGG 
GGCGCGCAGG TGCAGCAGGT GGTCAACATA GAGTGCGTGT CCGACTTCAC GGAGGCGCCA 
GTCCTCAACA TTCAGTTCAG GTATGGGGGC ACCTTCCAGA ACGTGTCTGT GCAGCTGCCC 
ATCACTCTCA ACAAATTCTT CCAGCCGACA GAAATGGCTT CTCAGGATTT CTTTCAACGT 
TGGAAGCAGT TGAGCAATCC ACAGCAGGAA GTGCAGAACA TCTTCAAAGC AAAGCACCCA 
ATGGACACAG AAGTCACCAA AGCCAAGATC ATTGGATTTG GTTCTGCACT TCTTGAAGAA 
GTTGATCCTA ATCCTGCGAA TTTCGTGGGA GCTGGAATCA TCCACACGAA AACCACCCAG 
ATTGGATGCC TGCTGCGCTT GGAGCCGAAC CTGCAAGCCC AGATGTACCG GCTCACGCTG 
CGCACAAGTA AGGAAGCCGT TTCTCAGAGA TTATGTGAAT TGCTCTCAGC GCAGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036437.1
CDS	214..3033
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIASVGSREMAEAFAGEIPKVLVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPDPAVRGRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAPQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFHLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKPTIQDVLRSDSQLRNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEDTKRDRSVDVNGGPEPAPASTSAVSTPSPSADLLGLGAAPPAPAGPPPSSGGSGLLVDVFSDSASVVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICSDDLQPNLNLQTKPVDPTVEGGAQVQQVVNIECVSDFTEAPVLNIQFRYGGTFQNVSVQLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEVTKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKEAVSQRLCELLSAQF

Target ORF information:

RefSeq Version	NM_012305.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 2 subunit alpha 2 (AP2A2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012305.3

ORF Insert Sequence:

ATGCCGGCCG TGTCCAAGGG GGACGGGATG CGGGGCCTGG CGGTCTTCAT CTCGGATATC 
CGCAACTGTA AAAGTAAAGA AGCAGAAATA AAAAGGATAA ACAAGGAACT GGCAAATATC 
AGATCAAAAT TTAAAGGTGA CAAGGCTCTT GATGGCTATA GTAAAAAAAA GTACGTCTGC 
AAGTTGCTCT TCATCTTTCT CCTTGGTCAT GACATTGACT TTGGACACAT GGAGGCTGTG 
AACCTGCTGA GTTCAAACAG ATACACGGAA AAGCAGATCG GCTACCTTTT CATCTCTGTG 
TTGGTGAACT CAAACAGTGA GCTGATCCGC CTGATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGGGCCTG GCCCTGCACT GCATCGCCAG CGTGGGCAGC 
CGGGAGATGG CCGAGGCCTT CGCCGGGGAG ATCCCTAAGG TCCTCGTAGC CGGAGACACT 
ATGGACAGCG TGAAGCAGAG CGCGGCCCTG TGCTTGCTGC GCCTGTACAG GACGTCCCCC 
GATCTTGTCC CCATGGGCGA CTGGACATCC CGAGTGGTGC ACCTGCTCAA TGACCAGCAC 
TTGGGTGTGG TAACTGCAGC CACAAGTCTG ATCACCACTT TAGCACAGAA GAACCCAGAA 
GAGTTTAAAA CCTCCGTGTC TCTGGCTGTC TCTAGGCTAA GCAGAATCGT GACGTCTGCA 
TCCACAGATC TCCAGGATTA CACTTACTAT TTTGTCCCGG CTCCCTGGCT GTCTGTCAAA 
CTGCTGAGAC TGCTGCAGTG CTACCCACCC CCAGACCCTG CAGTGCGAGG CCGCCTGACT 
GAGTGCCTGG AGACCATCCT GAACAAAGCC CAAGAACCGC CCAAGTCGAA GAAGGTCCAG 
CACTCCAACG CGAAGAATGC CGTGCTCTTC GAGGCCATCA GCTTAATCAT TCACCATGAC 
AGTGAGCCGA ACCTGCTCGT CCGTGCCTGC AACCAGTTGG GCCAGTTTCT GCAGCACCGC 
GAGACCAACC TGCGCTACCT GGCCCTGGAG AGCATGTGCA CGCTGGCCAG CTCTGAGTTC 
TCCCATGAGG CTGTCAAGAC GCACATCGAG ACGGTCATCA ACGCCCTGAA GACTGAGCGG 
GACGTGAGCG TGCGGCAGCG GGCCGTGGAC CTCCTCTACG CCATGTGCGA CCGCAGCAAC 
GCCCCACAGA TCGTGGCCGA GATGCTGAGC TATCTGGAGA CAGCTGACTA CTCCATCCGA 
GAAGAGATTG TGCTGAAGGT CGCCATCCTG GCTGAGAAGT ACGCGGTGGA CTACACCTGG 
TATGTGGATA CCATCTTGAA CTTGATCCGA ATTGCTGGTG ATTACGTGAG TGAAGAGGTG 
TGGTACCGAG TCATTCAGAT CGTCATCAAC CGGGACGACG TGCAGGGCTA CGCGGCCAAG 
ACTGTGTTCG AGGCTCTTCA GGCTCCCGCG TGCCACGAGA ACCTGGTCAA AGTGGGCGGC 
TACATCCTGG GGGAGTTTGG AAACTTGATA GCTGGAGACC CGAGATCCAG CCCGCTGATC 
CAGTTCCACC TGCTGCACTC CAAGTTCCAC CTGTGCAGCG TCCCCACCCG CGCGCTGCTC 
CTGTCCACCT ACATCAAGTT CGTGAACCTC TTCCCGGAGG TGAAGCCCAC CATCCAGGAC 
GTGCTGCGCA GCGACAGCCA GCTCAGGAAC GCAGACGTGG AGCTGCAGCA GCGTGCTGTG 
GAGTACCTGC GGCTCAGCAC CGTGGCCAGC ACCGACATTC TGGCGACCGT GCTGGAGGAG 
ATGCCCCCAT TCCCGGAGCG GGAGTCCTCC ATCTTGGCAA AGCTCAAGAA GAAGAAGGGC 
CCCAGCACGG TGACAGACCT GGAGGACACC AAGCGGGACA GGAGTGTGGA CGTGAACGGG 
GGTCCTGAGC CTGCCCCAGC CAGTACCAGC GCCGTGTCTA CGCCTTCTCC GTCGGCAGAC 
CTGCTGGGTC TCGGGGCTGC CCCCCCTGCC CCCGCGGGCC CCCCACCCTC CTCCGGCGGC 
AGCGGGCTGC TCGTGGACGT GTTCTCAGAC TCGGCCTCTG TGGTCGCGCC TCTCGCTCCT 
GGCTCCGAAG ACAACTTTGC CAGGTTTGTT TGTAAAAACA ATGGTGTGTT GTTTGAAAAC 
CAGCTGCTTC AAATTGGACT TAAGTCTGAA TTTCGGCAGA ATTTAGGTCG GATGTTTATC 
TTTTATGGTA ATAAGACCTC CACGCAGTTC CTAAACTTTA CCCCAACACT AATCTGTTCA 
GACGACCTTC AGCCTAACCT GAACCTGCAG ACCAAGCCCG TGGACCCGAC CGTGGAGGGG 
GGCGCGCAGG TGCAGCAGGT GGTCAACATA GAGTGCGTGT CCGACTTCAC GGAGGCGCCA 
GTCCTCAACA TTCAGTTCAG GTATGGGGGC ACCTTCCAGA ACGTGTCTGT GCAGCTGCCC 
ATCACTCTCA ACAAATTCTT CCAGCCGACA GAAATGGCTT CTCAGGATTT CTTTCAACGT 
TGGAAGCAGT TGAGCAATCC ACAGCAGGAA GTGCAGAACA TCTTCAAAGC AAAGCACCCA 
ATGGACACAG AAGTCACCAA AGCCAAGATC ATTGGATTTG GTTCTGCACT TCTTGAAGAA 
GTTGATCCTA ATCCTGCGAA TTTCGTGGGA GCTGGAATCA TCCACACGAA AACCACCCAG 
ATTGGATGCC TGCTGCGCTT GGAGCCGAAC CTGCAAGCCC AGATGTACCG GCTCACGCTG 
CGCACAAGTA AGGAAGCCGT TTCTCAGAGA TTATGTGAAT TGCTCTCAGC GCAGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP2A2 ( NM_012305.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036437.1 Homo sapiens adaptor related protein complex 2 subunit alpha 2 (AP2A2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21793
Clone ID Related Accession (Same CDS sequence)	NM_012305.3 , NM_012305.4
Accession Version	NM_012305.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	AP-2 complex subunit alpha-2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB461570.1, BC006155.1, AP006477.2 and BU735076.1. On Jun 2, 2019 this sequence version replaced NM_012305.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB020706.2, BC006155.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGGCCG TGTCCAAGGG GGACGGGATG CGGGGCCTGG CGGTCTTCAT CTCGGATATC 
CGCAACTGTA AAAGTAAAGA AGCAGAAATA AAAAGGATAA ACAAGGAACT GGCAAATATC 
AGATCAAAAT TTAAAGGTGA CAAGGCTCTT GATGGCTATA GTAAAAAAAA GTACGTCTGC 
AAGTTGCTCT TCATCTTTCT CCTTGGTCAT GACATTGACT TTGGACACAT GGAGGCTGTG 
AACCTGCTGA GTTCAAACAG ATACACGGAA AAGCAGATCG GCTACCTTTT CATCTCTGTG 
TTGGTGAACT CAAACAGTGA GCTGATCCGC CTGATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGGGCCTG GCCCTGCACT GCATCGCCAG CGTGGGCAGC 
CGGGAGATGG CCGAGGCCTT CGCCGGGGAG ATCCCTAAGG TCCTCGTAGC CGGAGACACT 
ATGGACAGCG TGAAGCAGAG CGCGGCCCTG TGCTTGCTGC GCCTGTACAG GACGTCCCCC 
GATCTTGTCC CCATGGGCGA CTGGACATCC CGAGTGGTGC ACCTGCTCAA TGACCAGCAC 
TTGGGTGTGG TAACTGCAGC CACAAGTCTG ATCACCACTT TAGCACAGAA GAACCCAGAA 
GAGTTTAAAA CCTCCGTGTC TCTGGCTGTC TCTAGGCTAA GCAGAATCGT GACGTCTGCA 
TCCACAGATC TCCAGGATTA CACTTACTAT TTTGTCCCGG CTCCCTGGCT GTCTGTCAAA 
CTGCTGAGAC TGCTGCAGTG CTACCCACCC CCAGACCCTG CAGTGCGAGG CCGCCTGACT 
GAGTGCCTGG AGACCATCCT GAACAAAGCC CAAGAACCGC CCAAGTCGAA GAAGGTCCAG 
CACTCCAACG CGAAGAATGC CGTGCTCTTC GAGGCCATCA GCTTAATCAT TCACCATGAC 
AGTGAGCCGA ACCTGCTCGT CCGTGCCTGC AACCAGTTGG GCCAGTTTCT GCAGCACCGC 
GAGACCAACC TGCGCTACCT GGCCCTGGAG AGCATGTGCA CGCTGGCCAG CTCTGAGTTC 
TCCCATGAGG CTGTCAAGAC GCACATCGAG ACGGTCATCA ACGCCCTGAA GACTGAGCGG 
GACGTGAGCG TGCGGCAGCG GGCCGTGGAC CTCCTCTACG CCATGTGCGA CCGCAGCAAC 
GCCCCACAGA TCGTGGCCGA GATGCTGAGC TATCTGGAGA CAGCTGACTA CTCCATCCGA 
GAAGAGATTG TGCTGAAGGT CGCCATCCTG GCTGAGAAGT ACGCGGTGGA CTACACCTGG 
TATGTGGATA CCATCTTGAA CTTGATCCGA ATTGCTGGTG ATTACGTGAG TGAAGAGGTG 
TGGTACCGAG TCATTCAGAT CGTCATCAAC CGGGACGACG TGCAGGGCTA CGCGGCCAAG 
ACTGTGTTCG AGGCTCTTCA GGCTCCCGCG TGCCACGAGA ACCTGGTCAA AGTGGGCGGC 
TACATCCTGG GGGAGTTTGG AAACTTGATA GCTGGAGACC CGAGATCCAG CCCGCTGATC 
CAGTTCCACC TGCTGCACTC CAAGTTCCAC CTGTGCAGCG TCCCCACCCG CGCGCTGCTC 
CTGTCCACCT ACATCAAGTT CGTGAACCTC TTCCCGGAGG TGAAGCCCAC CATCCAGGAC 
GTGCTGCGCA GCGACAGCCA GCTCAGGAAC GCAGACGTGG AGCTGCAGCA GCGTGCTGTG 
GAGTACCTGC GGCTCAGCAC CGTGGCCAGC ACCGACATTC TGGCGACCGT GCTGGAGGAG 
ATGCCCCCAT TCCCGGAGCG GGAGTCCTCC ATCTTGGCAA AGCTCAAGAA GAAGAAGGGC 
CCCAGCACGG TGACAGACCT GGAGGACACC AAGCGGGACA GGAGTGTGGA CGTGAACGGG 
GGTCCTGAGC CTGCCCCAGC CAGTACCAGC GCCGTGTCTA CGCCTTCTCC GTCGGCAGAC 
CTGCTGGGTC TCGGGGCTGC CCCCCCTGCC CCCGCGGGCC CCCCACCCTC CTCCGGCGGC 
AGCGGGCTGC TCGTGGACGT GTTCTCAGAC TCGGCCTCTG TGGTCGCGCC TCTCGCTCCT 
GGCTCCGAAG ACAACTTTGC CAGGTTTGTT TGTAAAAACA ATGGTGTGTT GTTTGAAAAC 
CAGCTGCTTC AAATTGGACT TAAGTCTGAA TTTCGGCAGA ATTTAGGTCG GATGTTTATC 
TTTTATGGTA ATAAGACCTC CACGCAGTTC CTAAACTTTA CCCCAACACT AATCTGTTCA 
GACGACCTTC AGCCTAACCT GAACCTGCAG ACCAAGCCCG TGGACCCGAC CGTGGAGGGG 
GGCGCGCAGG TGCAGCAGGT GGTCAACATA GAGTGCGTGT CCGACTTCAC GGAGGCGCCA 
GTCCTCAACA TTCAGTTCAG GTATGGGGGC ACCTTCCAGA ACGTGTCTGT GCAGCTGCCC 
ATCACTCTCA ACAAATTCTT CCAGCCGACA GAAATGGCTT CTCAGGATTT CTTTCAACGT 
TGGAAGCAGT TGAGCAATCC ACAGCAGGAA GTGCAGAACA TCTTCAAAGC AAAGCACCCA 
ATGGACACAG AAGTCACCAA AGCCAAGATC ATTGGATTTG GTTCTGCACT TCTTGAAGAA 
GTTGATCCTA ATCCTGCGAA TTTCGTGGGA GCTGGAATCA TCCACACGAA AACCACCCAG 
ATTGGATGCC TGCTGCGCTT GGAGCCGAAC CTGCAAGCCC AGATGTACCG GCTCACGCTG 
CGCACAAGTA AGGAAGCCGT TTCTCAGAGA TTATGTGAAT TGCTCTCAGC GCAGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036437.1
CDS	153..2972
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIASVGSREMAEAFAGEIPKVLVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPDPAVRGRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAPQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFHLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKPTIQDVLRSDSQLRNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEDTKRDRSVDVNGGPEPAPASTSAVSTPSPSADLLGLGAAPPAPAGPPPSSGGSGLLVDVFSDSASVVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICSDDLQPNLNLQTKPVDPTVEGGAQVQQVVNIECVSDFTEAPVLNIQFRYGGTFQNVSVQLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEVTKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKEAVSQRLCELLSAQF

Target ORF information:

RefSeq Version	NM_012305.4
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 2 subunit alpha 2 (AP2A2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012305.4

ORF Insert Sequence:

ATGCCGGCCG TGTCCAAGGG GGACGGGATG CGGGGCCTGG CGGTCTTCAT CTCGGATATC 
CGCAACTGTA AAAGTAAAGA AGCAGAAATA AAAAGGATAA ACAAGGAACT GGCAAATATC 
AGATCAAAAT TTAAAGGTGA CAAGGCTCTT GATGGCTATA GTAAAAAAAA GTACGTCTGC 
AAGTTGCTCT TCATCTTTCT CCTTGGTCAT GACATTGACT TTGGACACAT GGAGGCTGTG 
AACCTGCTGA GTTCAAACAG ATACACGGAA AAGCAGATCG GCTACCTTTT CATCTCTGTG 
TTGGTGAACT CAAACAGTGA GCTGATCCGC CTGATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGGGCCTG GCCCTGCACT GCATCGCCAG CGTGGGCAGC 
CGGGAGATGG CCGAGGCCTT CGCCGGGGAG ATCCCTAAGG TCCTCGTAGC CGGAGACACT 
ATGGACAGCG TGAAGCAGAG CGCGGCCCTG TGCTTGCTGC GCCTGTACAG GACGTCCCCC 
GATCTTGTCC CCATGGGCGA CTGGACATCC CGAGTGGTGC ACCTGCTCAA TGACCAGCAC 
TTGGGTGTGG TAACTGCAGC CACAAGTCTG ATCACCACTT TAGCACAGAA GAACCCAGAA 
GAGTTTAAAA CCTCCGTGTC TCTGGCTGTC TCTAGGCTAA GCAGAATCGT GACGTCTGCA 
TCCACAGATC TCCAGGATTA CACTTACTAT TTTGTCCCGG CTCCCTGGCT GTCTGTCAAA 
CTGCTGAGAC TGCTGCAGTG CTACCCACCC CCAGACCCTG CAGTGCGAGG CCGCCTGACT 
GAGTGCCTGG AGACCATCCT GAACAAAGCC CAAGAACCGC CCAAGTCGAA GAAGGTCCAG 
CACTCCAACG CGAAGAATGC CGTGCTCTTC GAGGCCATCA GCTTAATCAT TCACCATGAC 
AGTGAGCCGA ACCTGCTCGT CCGTGCCTGC AACCAGTTGG GCCAGTTTCT GCAGCACCGC 
GAGACCAACC TGCGCTACCT GGCCCTGGAG AGCATGTGCA CGCTGGCCAG CTCTGAGTTC 
TCCCATGAGG CTGTCAAGAC GCACATCGAG ACGGTCATCA ACGCCCTGAA GACTGAGCGG 
GACGTGAGCG TGCGGCAGCG GGCCGTGGAC CTCCTCTACG CCATGTGCGA CCGCAGCAAC 
GCCCCACAGA TCGTGGCCGA GATGCTGAGC TATCTGGAGA CAGCTGACTA CTCCATCCGA 
GAAGAGATTG TGCTGAAGGT CGCCATCCTG GCTGAGAAGT ACGCGGTGGA CTACACCTGG 
TATGTGGATA CCATCTTGAA CTTGATCCGA ATTGCTGGTG ATTACGTGAG TGAAGAGGTG 
TGGTACCGAG TCATTCAGAT CGTCATCAAC CGGGACGACG TGCAGGGCTA CGCGGCCAAG 
ACTGTGTTCG AGGCTCTTCA GGCTCCCGCG TGCCACGAGA ACCTGGTCAA AGTGGGCGGC 
TACATCCTGG GGGAGTTTGG AAACTTGATA GCTGGAGACC CGAGATCCAG CCCGCTGATC 
CAGTTCCACC TGCTGCACTC CAAGTTCCAC CTGTGCAGCG TCCCCACCCG CGCGCTGCTC 
CTGTCCACCT ACATCAAGTT CGTGAACCTC TTCCCGGAGG TGAAGCCCAC CATCCAGGAC 
GTGCTGCGCA GCGACAGCCA GCTCAGGAAC GCAGACGTGG AGCTGCAGCA GCGTGCTGTG 
GAGTACCTGC GGCTCAGCAC CGTGGCCAGC ACCGACATTC TGGCGACCGT GCTGGAGGAG 
ATGCCCCCAT TCCCGGAGCG GGAGTCCTCC ATCTTGGCAA AGCTCAAGAA GAAGAAGGGC 
CCCAGCACGG TGACAGACCT GGAGGACACC AAGCGGGACA GGAGTGTGGA CGTGAACGGG 
GGTCCTGAGC CTGCCCCAGC CAGTACCAGC GCCGTGTCTA CGCCTTCTCC GTCGGCAGAC 
CTGCTGGGTC TCGGGGCTGC CCCCCCTGCC CCCGCGGGCC CCCCACCCTC CTCCGGCGGC 
AGCGGGCTGC TCGTGGACGT GTTCTCAGAC TCGGCCTCTG TGGTCGCGCC TCTCGCTCCT 
GGCTCCGAAG ACAACTTTGC CAGGTTTGTT TGTAAAAACA ATGGTGTGTT GTTTGAAAAC 
CAGCTGCTTC AAATTGGACT TAAGTCTGAA TTTCGGCAGA ATTTAGGTCG GATGTTTATC 
TTTTATGGTA ATAAGACCTC CACGCAGTTC CTAAACTTTA CCCCAACACT AATCTGTTCA 
GACGACCTTC AGCCTAACCT GAACCTGCAG ACCAAGCCCG TGGACCCGAC CGTGGAGGGG 
GGCGCGCAGG TGCAGCAGGT GGTCAACATA GAGTGCGTGT CCGACTTCAC GGAGGCGCCA 
GTCCTCAACA TTCAGTTCAG GTATGGGGGC ACCTTCCAGA ACGTGTCTGT GCAGCTGCCC 
ATCACTCTCA ACAAATTCTT CCAGCCGACA GAAATGGCTT CTCAGGATTT CTTTCAACGT 
TGGAAGCAGT TGAGCAATCC ACAGCAGGAA GTGCAGAACA TCTTCAAAGC AAAGCACCCA 
ATGGACACAG AAGTCACCAA AGCCAAGATC ATTGGATTTG GTTCTGCACT TCTTGAAGAA 
GTTGATCCTA ATCCTGCGAA TTTCGTGGGA GCTGGAATCA TCCACACGAA AACCACCCAG 
ATTGGATGCC TGCTGCGCTT GGAGCCGAAC CTGCAAGCCC AGATGTACCG GCTCACGCTG 
CGCACAAGTA AGGAAGCCGT TTCTCAGAGA TTATGTGAAT TGCTCTCAGC GCAGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP2A2 ( XM_011519930.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518232.1 Homo sapiens adaptor related protein complex 2 subunit alpha 2 (AP2A2), transcript variant X2, mRNA.

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CloneID	OHu56208
Clone ID Related Accession (Same CDS sequence)	XM_011519930.3 , XM_011519930.2
Accession Version	XM_011519930.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	AP-2 complex subunit alpha-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011519930.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGCCG TGTCCAAGGG GGACGGGATG CGGGGCCTGG CGGTCTTCAT CTCGGATATC 
CGCAACTGTA AAAGTAAAGA AGCAGAAATA AAAAGGATAA ACAAGGAACT GGCAAATATC 
AGATCAAAAT TTAAAGGTGA CAAGGCTCTT GATGGCTATA GTAAAAAAAA GTACGTCTGC 
AAGTTGCTCT TCATCTTTCT CCTTGGTCAT GACATTGACT TTGGACACAT GGAGGCTGTG 
AACCTGCTGA GTTCAAACAG ATACACGGAA AAGCAGATCG GCTACCTTTT CATCTCTGTG 
TTGGTGAACT CAAACAGTGA GCTGATCCGC CTGATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGGGCCTG GCCCTGCACT GCATCGCCAG CGTGGGCAGC 
CGGGAGATGG CCGAGGCCTT CGCCGGGGAG ATCCCTAAGG TCCTCGTAGC CGGAGACACT 
ATGGACAGCG TGAAGCAGAG CGCGGCCCTG TGCTTGCTGC GCCTGTACAG GACGTCCCCC 
GATCTTGTCC CCATGGGCGA CTGGACATCC CGAGTGGTGC ACCTGCTCAA TGACCAGCAC 
TTGGGTGTGG TAACTGCAGC CACAAGTCTG ATCACCACTT TAGCACAGAA GAACCCAGAA 
GAGTTTAAAA CCTCCGTGTC TCTGGCTGTC TCTAGGCTAA GCAGAATCGT GACGTCTGCA 
TCCACAGATC TCCAGGATTA CACTTACTAT TTTGTCCCGG CTCCCTGGCT GTCTGTCAAA 
CTGCTGAGAC TGCTGCAGTG CTACCCACCC CCAGAAGACC CTGCAGTGCG AGGCCGCCTG 
ACTGAGTGCC TGGAGACCAT CCTGAACAAA GCCCAAGAAC CGCCCAAGTC GAAGAAGGTC 
CAGCACTCCA ACGCGAAGAA TGCCGTGCTC TTCGAGGCCA TCAGCTTAAT CATTCACCAT 
GACAGTGAGC CGAACCTGCT CGTCCGTGCC TGCAACCAGT TGGGCCAGTT TCTGCAGCAC 
CGCGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCTGAG 
TTCTCCCATG AGGCTGTCAA GACGCACATC GAGACGGTCA TCAACGCCCT GAAGACTGAG 
CGGGACGTGA GCGTGCGGCA GCGGGCCGTG GACCTCCTCT ACGCCATGTG CGACCGCAGC 
AACGCCCCAC AGATCGTGGC CGAGATGCTG AGCTATCTGG AGACAGCTGA CTACTCCATC 
CGAGAAGAGA TTGTGCTGAA GGTCGCCATC CTGGCTGAGA AGTACGCGGT GGACTACACC 
TGGTATGTGG ATACCATCTT GAACTTGATC CGAATTGCTG GTGATTACGT GAGTGAAGAG 
GTGTGGTACC GAGTCATTCA GATCGTCATC AACCGGGACG ACGTGCAGGG CTACGCGGCC 
AAGACTGTGT TCGAGGCTCT TCAGGCTCCC GCGTGCCACG AGAACCTGGT CAAAGTGGGC 
GGCTACATCC TGGGGGAGTT TGGAAACTTG ATAGCTGGAG ACCCGAGATC CAGCCCGCTG 
ATCCAGTTCC ACCTGCTGCA CTCCAAGTTC CACCTGTGCA GCGTCCCCAC CCGCGCGCTG 
CTCCTGTCCA CCTACATCAA GTTCGTGAAC CTCTTCCCGG AGGTGAAGCC CACCATCCAG 
GACGTGCTGC GCAGCGACAG CCAGCTCAGG AACGCAGACG TGGAGCTGCA GCAGCGTGCT 
GTGGAGTACC TGCGGCTCAG CACCGTGGCC AGCACCGACA TTCTGGCGAC CGTGCTGGAG 
GAGATGCCCC CATTCCCGGA GCGGGAGTCC TCCATCTTGG CAAAGCTCAA GAAGAAGAAG 
GGCCCCAGCA CGGTGACAGA CCTGGAGGAC ACCAAGCGGG ACAGGAGTGT GGACGTGAAC 
GGGGGTCCTG AGCCTGCCCC AGCCAGTACC AGCGCCGTGG TTTCTGGAGG CAGAAACTTG 
CTTGTGGAGA TTTGTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518232.1
CDS	173..2173
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIASVGSREMAEAFAGEIPKVLVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDPAVRGRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAPQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFHLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKPTIQDVLRSDSQLRNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEDTKRDRSVDVNGGPEPAPASTSAVVSGGRNLLVEICL

Target ORF information:

RefSeq Version	XM_011519930.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 2 subunit alpha 2 (AP2A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519930.3

ORF Insert Sequence:

ATGCCGGCCG TGTCCAAGGG GGACGGGATG CGGGGCCTGG CGGTCTTCAT CTCGGATATC 
CGCAACTGTA AAAGTAAAGA AGCAGAAATA AAAAGGATAA ACAAGGAACT GGCAAATATC 
AGATCAAAAT TTAAAGGTGA CAAGGCTCTT GATGGCTATA GTAAAAAAAA GTACGTCTGC 
AAGTTGCTCT TCATCTTTCT CCTTGGTCAT GACATTGACT TTGGACACAT GGAGGCTGTG 
AACCTGCTGA GTTCAAACAG ATACACGGAA AAGCAGATCG GCTACCTTTT CATCTCTGTG 
TTGGTGAACT CAAACAGTGA GCTGATCCGC CTGATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGGGCCTG GCCCTGCACT GCATCGCCAG CGTGGGCAGC 
CGGGAGATGG CCGAGGCCTT CGCCGGGGAG ATCCCTAAGG TCCTCGTAGC CGGAGACACT 
ATGGACAGCG TGAAGCAGAG CGCGGCCCTG TGCTTGCTGC GCCTGTACAG GACGTCCCCC 
GATCTTGTCC CCATGGGCGA CTGGACATCC CGAGTGGTGC ACCTGCTCAA TGACCAGCAC 
TTGGGTGTGG TAACTGCAGC CACAAGTCTG ATCACCACTT TAGCACAGAA GAACCCAGAA 
GAGTTTAAAA CCTCCGTGTC TCTGGCTGTC TCTAGGCTAA GCAGAATCGT GACGTCTGCA 
TCCACAGATC TCCAGGATTA CACTTACTAT TTTGTCCCGG CTCCCTGGCT GTCTGTCAAA 
CTGCTGAGAC TGCTGCAGTG CTACCCACCC CCAGAAGACC CTGCAGTGCG AGGCCGCCTG 
ACTGAGTGCC TGGAGACCAT CCTGAACAAA GCCCAAGAAC CGCCCAAGTC GAAGAAGGTC 
CAGCACTCCA ACGCGAAGAA TGCCGTGCTC TTCGAGGCCA TCAGCTTAAT CATTCACCAT 
GACAGTGAGC CGAACCTGCT CGTCCGTGCC TGCAACCAGT TGGGCCAGTT TCTGCAGCAC 
CGCGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCTGAG 
TTCTCCCATG AGGCTGTCAA GACGCACATC GAGACGGTCA TCAACGCCCT GAAGACTGAG 
CGGGACGTGA GCGTGCGGCA GCGGGCCGTG GACCTCCTCT ACGCCATGTG CGACCGCAGC 
AACGCCCCAC AGATCGTGGC CGAGATGCTG AGCTATCTGG AGACAGCTGA CTACTCCATC 
CGAGAAGAGA TTGTGCTGAA GGTCGCCATC CTGGCTGAGA AGTACGCGGT GGACTACACC 
TGGTATGTGG ATACCATCTT GAACTTGATC CGAATTGCTG GTGATTACGT GAGTGAAGAG 
GTGTGGTACC GAGTCATTCA GATCGTCATC AACCGGGACG ACGTGCAGGG CTACGCGGCC 
AAGACTGTGT TCGAGGCTCT TCAGGCTCCC GCGTGCCACG AGAACCTGGT CAAAGTGGGC 
GGCTACATCC TGGGGGAGTT TGGAAACTTG ATAGCTGGAG ACCCGAGATC CAGCCCGCTG 
ATCCAGTTCC ACCTGCTGCA CTCCAAGTTC CACCTGTGCA GCGTCCCCAC CCGCGCGCTG 
CTCCTGTCCA CCTACATCAA GTTCGTGAAC CTCTTCCCGG AGGTGAAGCC CACCATCCAG 
GACGTGCTGC GCAGCGACAG CCAGCTCAGG AACGCAGACG TGGAGCTGCA GCAGCGTGCT 
GTGGAGTACC TGCGGCTCAG CACCGTGGCC AGCACCGACA TTCTGGCGAC CGTGCTGGAG 
GAGATGCCCC CATTCCCGGA GCGGGAGTCC TCCATCTTGG CAAAGCTCAA GAAGAAGAAG 
GGCCCCAGCA CGGTGACAGA CCTGGAGGAC ACCAAGCGGG ACAGGAGTGT GGACGTGAAC 
GGGGGTCCTG AGCCTGCCCC AGCCAGTACC AGCGCCGTGG TTTCTGGAGG CAGAAACTTG 
CTTGTGGAGA TTTGTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP2A2 ( XM_011519930.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518232.1 Homo sapiens adaptor related protein complex 2 alpha 2 subunit (AP2A2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu56208
Clone ID Related Accession (Same CDS sequence)	XM_011519930.3 , XM_011519930.2
Accession Version	XM_011519930.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	AP-2 complex subunit alpha-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519930.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGCCG TGTCCAAGGG GGACGGGATG CGGGGCCTGG CGGTCTTCAT CTCGGATATC 
CGCAACTGTA AAAGTAAAGA AGCAGAAATA AAAAGGATAA ACAAGGAACT GGCAAATATC 
AGATCAAAAT TTAAAGGTGA CAAGGCTCTT GATGGCTATA GTAAAAAAAA GTACGTCTGC 
AAGTTGCTCT TCATCTTTCT CCTTGGTCAT GACATTGACT TTGGACACAT GGAGGCTGTG 
AACCTGCTGA GTTCAAACAG ATACACGGAA AAGCAGATCG GCTACCTTTT CATCTCTGTG 
TTGGTGAACT CAAACAGTGA GCTGATCCGC CTGATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGGGCCTG GCCCTGCACT GCATCGCCAG CGTGGGCAGC 
CGGGAGATGG CCGAGGCCTT CGCCGGGGAG ATCCCTAAGG TCCTCGTAGC CGGAGACACT 
ATGGACAGCG TGAAGCAGAG CGCGGCCCTG TGCTTGCTGC GCCTGTACAG GACGTCCCCC 
GATCTTGTCC CCATGGGCGA CTGGACATCC CGAGTGGTGC ACCTGCTCAA TGACCAGCAC 
TTGGGTGTGG TAACTGCAGC CACAAGTCTG ATCACCACTT TAGCACAGAA GAACCCAGAA 
GAGTTTAAAA CCTCCGTGTC TCTGGCTGTC TCTAGGCTAA GCAGAATCGT GACGTCTGCA 
TCCACAGATC TCCAGGATTA CACTTACTAT TTTGTCCCGG CTCCCTGGCT GTCTGTCAAA 
CTGCTGAGAC TGCTGCAGTG CTACCCACCC CCAGAAGACC CTGCAGTGCG AGGCCGCCTG 
ACTGAGTGCC TGGAGACCAT CCTGAACAAA GCCCAAGAAC CGCCCAAGTC GAAGAAGGTC 
CAGCACTCCA ACGCGAAGAA TGCCGTGCTC TTCGAGGCCA TCAGCTTAAT CATTCACCAT 
GACAGTGAGC CGAACCTGCT CGTCCGTGCC TGCAACCAGT TGGGCCAGTT TCTGCAGCAC 
CGCGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCTGAG 
TTCTCCCATG AGGCTGTCAA GACGCACATC GAGACGGTCA TCAACGCCCT GAAGACTGAG 
CGGGACGTGA GCGTGCGGCA GCGGGCCGTG GACCTCCTCT ACGCCATGTG CGACCGCAGC 
AACGCCCCAC AGATCGTGGC CGAGATGCTG AGCTATCTGG AGACAGCTGA CTACTCCATC 
CGAGAAGAGA TTGTGCTGAA GGTCGCCATC CTGGCTGAGA AGTACGCGGT GGACTACACC 
TGGTATGTGG ATACCATCTT GAACTTGATC CGAATTGCTG GTGATTACGT GAGTGAAGAG 
GTGTGGTACC GAGTCATTCA GATCGTCATC AACCGGGACG ACGTGCAGGG CTACGCGGCC 
AAGACTGTGT TCGAGGCTCT TCAGGCTCCC GCGTGCCACG AGAACCTGGT CAAAGTGGGC 
GGCTACATCC TGGGGGAGTT TGGAAACTTG ATAGCTGGAG ACCCGAGATC CAGCCCGCTG 
ATCCAGTTCC ACCTGCTGCA CTCCAAGTTC CACCTGTGCA GCGTCCCCAC CCGCGCGCTG 
CTCCTGTCCA CCTACATCAA GTTCGTGAAC CTCTTCCCGG AGGTGAAGCC CACCATCCAG 
GACGTGCTGC GCAGCGACAG CCAGCTCAGG AACGCAGACG TGGAGCTGCA GCAGCGTGCT 
GTGGAGTACC TGCGGCTCAG CACCGTGGCC AGCACCGACA TTCTGGCGAC CGTGCTGGAG 
GAGATGCCCC CATTCCCGGA GCGGGAGTCC TCCATCTTGG CAAAGCTCAA GAAGAAGAAG 
GGCCCCAGCA CGGTGACAGA CCTGGAGGAC ACCAAGCGGG ACAGGAGTGT GGACGTGAAC 
GGGGGTCCTG AGCCTGCCCC AGCCAGTACC AGCGCCGTGG TTTCTGGAGG CAGAAACTTG 
CTTGTGGAGA TTTGTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518232.1
CDS	175..2175
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIASVGSREMAEAFAGEIPKVLVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDPAVRGRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAPQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFHLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKPTIQDVLRSDSQLRNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEDTKRDRSVDVNGGPEPAPASTSAVVSGGRNLLVEICL

Target ORF information:

RefSeq Version	XM_011519930.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 2 alpha 2 subunit (AP2A2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519930.2

ORF Insert Sequence:

ATGCCGGCCG TGTCCAAGGG GGACGGGATG CGGGGCCTGG CGGTCTTCAT CTCGGATATC 
CGCAACTGTA AAAGTAAAGA AGCAGAAATA AAAAGGATAA ACAAGGAACT GGCAAATATC 
AGATCAAAAT TTAAAGGTGA CAAGGCTCTT GATGGCTATA GTAAAAAAAA GTACGTCTGC 
AAGTTGCTCT TCATCTTTCT CCTTGGTCAT GACATTGACT TTGGACACAT GGAGGCTGTG 
AACCTGCTGA GTTCAAACAG ATACACGGAA AAGCAGATCG GCTACCTTTT CATCTCTGTG 
TTGGTGAACT CAAACAGTGA GCTGATCCGC CTGATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGGGCCTG GCCCTGCACT GCATCGCCAG CGTGGGCAGC 
CGGGAGATGG CCGAGGCCTT CGCCGGGGAG ATCCCTAAGG TCCTCGTAGC CGGAGACACT 
ATGGACAGCG TGAAGCAGAG CGCGGCCCTG TGCTTGCTGC GCCTGTACAG GACGTCCCCC 
GATCTTGTCC CCATGGGCGA CTGGACATCC CGAGTGGTGC ACCTGCTCAA TGACCAGCAC 
TTGGGTGTGG TAACTGCAGC CACAAGTCTG ATCACCACTT TAGCACAGAA GAACCCAGAA 
GAGTTTAAAA CCTCCGTGTC TCTGGCTGTC TCTAGGCTAA GCAGAATCGT GACGTCTGCA 
TCCACAGATC TCCAGGATTA CACTTACTAT TTTGTCCCGG CTCCCTGGCT GTCTGTCAAA 
CTGCTGAGAC TGCTGCAGTG CTACCCACCC CCAGAAGACC CTGCAGTGCG AGGCCGCCTG 
ACTGAGTGCC TGGAGACCAT CCTGAACAAA GCCCAAGAAC CGCCCAAGTC GAAGAAGGTC 
CAGCACTCCA ACGCGAAGAA TGCCGTGCTC TTCGAGGCCA TCAGCTTAAT CATTCACCAT 
GACAGTGAGC CGAACCTGCT CGTCCGTGCC TGCAACCAGT TGGGCCAGTT TCTGCAGCAC 
CGCGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCTGAG 
TTCTCCCATG AGGCTGTCAA GACGCACATC GAGACGGTCA TCAACGCCCT GAAGACTGAG 
CGGGACGTGA GCGTGCGGCA GCGGGCCGTG GACCTCCTCT ACGCCATGTG CGACCGCAGC 
AACGCCCCAC AGATCGTGGC CGAGATGCTG AGCTATCTGG AGACAGCTGA CTACTCCATC 
CGAGAAGAGA TTGTGCTGAA GGTCGCCATC CTGGCTGAGA AGTACGCGGT GGACTACACC 
TGGTATGTGG ATACCATCTT GAACTTGATC CGAATTGCTG GTGATTACGT GAGTGAAGAG 
GTGTGGTACC GAGTCATTCA GATCGTCATC AACCGGGACG ACGTGCAGGG CTACGCGGCC 
AAGACTGTGT TCGAGGCTCT TCAGGCTCCC GCGTGCCACG AGAACCTGGT CAAAGTGGGC 
GGCTACATCC TGGGGGAGTT TGGAAACTTG ATAGCTGGAG ACCCGAGATC CAGCCCGCTG 
ATCCAGTTCC ACCTGCTGCA CTCCAAGTTC CACCTGTGCA GCGTCCCCAC CCGCGCGCTG 
CTCCTGTCCA CCTACATCAA GTTCGTGAAC CTCTTCCCGG AGGTGAAGCC CACCATCCAG 
GACGTGCTGC GCAGCGACAG CCAGCTCAGG AACGCAGACG TGGAGCTGCA GCAGCGTGCT 
GTGGAGTACC TGCGGCTCAG CACCGTGGCC AGCACCGACA TTCTGGCGAC CGTGCTGGAG 
GAGATGCCCC CATTCCCGGA GCGGGAGTCC TCCATCTTGG CAAAGCTCAA GAAGAAGAAG 
GGCCCCAGCA CGGTGACAGA CCTGGAGGAC ACCAAGCGGG ACAGGAGTGT GGACGTGAAC 
GGGGGTCCTG AGCCTGCCCC AGCCAGTACC AGCGCCGTGG TTTCTGGAGG CAGAAACTTG 
CTTGTGGAGA TTTGTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP2A2 ( NM_001242837.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229766.1 Homo sapiens adaptor related protein complex 2 subunit alpha 2 (AP2A2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22502
Clone ID Related Accession (Same CDS sequence)	NM_001242837.1
Accession Version	NM_001242837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	AP-2 complex subunit alpha-2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB461570.1, AK223558.1, AP006477.2 and BU735076.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK223558.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGCCG TGTCCAAGGG GGACGGGATG CGGGGCCTGG CGGTCTTCAT CTCGGATATC 
CGCAACTGTA AAAGTAAAGA AGCAGAAATA AAAAGGATAA ACAAGGAACT GGCAAATATC 
AGATCAAAAT TTAAAGGTGA CAAGGCTCTT GATGGCTATA GTAAAAAAAA GTACGTCTGC 
AAGTTGCTCT TCATCTTTCT CCTTGGTCAT GACATTGACT TTGGACACAT GGAGGCTGTG 
AACCTGCTGA GTTCAAACAG ATACACGGAA AAGCAGATCG GCTACCTTTT CATCTCTGTG 
TTGGTGAACT CAAACAGTGA GCTGATCCGC CTGATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGGGCCTG GCCCTGCACT GCATCGCCAG CGTGGGCAGC 
CGGGAGATGG CCGAGGCCTT CGCCGGGGAG ATCCCTAAGG TCCTCGTAGC CGGAGACACT 
ATGGACAGCG TGAAGCAGAG CGCGGCCCTG TGCTTGCTGC GCCTGTACAG GACGTCCCCC 
GATCTTGTCC CCATGGGCGA CTGGACATCC CGAGTGGTGC ACCTGCTCAA TGACCAGCAC 
TTGGGTGTGG TAACTGCAGC CACAAGTCTG ATCACCACTT TAGCACAGAA GAACCCAGAA 
GAGTTTAAAA CCTCCGTGTC TCTGGCTGTC TCTAGGCTAA GCAGAATCGT GACGTCTGCA 
TCCACAGATC TCCAGGATTA CACTTACTAT TTTGTCCCGG CTCCCTGGCT GTCTGTCAAA 
CTGCTGAGAC TGCTGCAGTG CTACCCACCC CCAGAAGACC CTGCAGTGCG AGGCCGCCTG 
ACTGAGTGCC TGGAGACCAT CCTGAACAAA GCCCAAGAAC CGCCCAAGTC GAAGAAGGTC 
CAGCACTCCA ACGCGAAGAA TGCCGTGCTC TTCGAGGCCA TCAGCTTAAT CATTCACCAT 
GACAGTGAGC CGAACCTGCT CGTCCGTGCC TGCAACCAGT TGGGCCAGTT TCTGCAGCAC 
CGCGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCTGAG 
TTCTCCCATG AGGCTGTCAA GACGCACATC GAGACGGTCA TCAACGCCCT GAAGACTGAG 
CGGGACGTGA GCGTGCGGCA GCGGGCCGTG GACCTCCTCT ACGCCATGTG CGACCGCAGC 
AACGCCCCAC AGATCGTGGC CGAGATGCTG AGCTATCTGG AGACAGCTGA CTACTCCATC 
CGAGAAGAGA TTGTGCTGAA GGTCGCCATC CTGGCTGAGA AGTACGCGGT GGACTACACC 
TGGTATGTGG ATACCATCTT GAACTTGATC CGAATTGCTG GTGATTACGT GAGTGAAGAG 
GTGTGGTACC GAGTCATTCA GATCGTCATC AACCGGGACG ACGTGCAGGG CTACGCGGCC 
AAGACTGTGT TCGAGGCTCT TCAGGCTCCC GCGTGCCACG AGAACCTGGT CAAAGTGGGC 
GGCTACATCC TGGGGGAGTT TGGAAACTTG ATAGCTGGAG ACCCGAGATC CAGCCCGCTG 
ATCCAGTTCC ACCTGCTGCA CTCCAAGTTC CACCTGTGCA GCGTCCCCAC CCGCGCGCTG 
CTCCTGTCCA CCTACATCAA GTTCGTGAAC CTCTTCCCGG AGGTGAAGCC CACCATCCAG 
GACGTGCTGC GCAGCGACAG CCAGCTCAGG AACGCAGACG TGGAGCTGCA GCAGCGTGCT 
GTGGAGTACC TGCGGCTCAG CACCGTGGCC AGCACCGACA TTCTGGCGAC CGTGCTGGAG 
GAGATGCCCC CATTCCCGGA GCGGGAGTCC TCCATCTTGG CAAAGCTCAA GAAGAAGAAG 
GGCCCCAGCA CGGTGACAGA CCTGGAGGAC ACCAAGCGGG ACAGGAGTGT GGACGTGAAC 
GGGGGTCCTG AGCCTGCCCC AGCCAGTACC AGCGCCGTGT CTACGCCTTC TCCGTCGGCA 
GACCTGCTGG GTCTCGGGGC TGCCCCCCCT GCCCCCGCGG GCCCCCCACC CTCCTCCGGC 
GGCAGCGGGC TGCTCGTGGA CGTGTTCTCA GACTCGGCCT CTGTGGTCGC GCCTCTCGCT 
CCTGGCTCCG AAGACAACTT TGCCAGGTTT GTTTGTAAAA ACAATGGTGT GTTGTTTGAA 
AACCAGCTGC TTCAAATTGG ACTTAAGTCT GAATTTCGGC AGAATTTAGG TCGGATGTTT 
ATCTTTTATG GTAATAAGAC CTCCACGCAG TTCCTAAACT TTACCCCAAC ACTAATCTGT 
TCAGACGACC TTCAGCCTAA CCTGAACCTG CAGACCAAGC CCGTGGACCC GACCGTGGAG 
GGGGGCGCGC AGGTGCAGCA GGTGGTCAAC ATAGAGTGCG TGTCCGACTT CACGGAGGCG 
CCAGTCCTCA ACATTCAGTT CAGGTATGGG GGCACCTTCC AGAACGTGTC TGTGCAGCTG 
CCCATCACTC TCAACAAATT CTTCCAGCCG ACAGAAATGG CTTCTCAGGA TTTCTTTCAA 
CGTTGGAAGC AGTTGAGCAA TCCACAGCAG GAAGTGCAGA ACATCTTCAA AGCAAAGCAC 
CCAATGGACA CAGAAGTCAC CAAAGCCAAG ATCATTGGAT TTGGTTCTGC ACTTCTTGAA 
GAAGTTGATC CTAATCCTGC GAATTTCGTG GGAGCTGGAA TCATCCACAC GAAAACCACC 
CAGATTGGAT GCCTGCTGCG CTTGGAGCCG AACCTGCAAG CCCAGATGTA CCGGCTCACG 
CTGCGCACAA GTAAGGAAGC CGTTTCTCAG AGATTATGTG AATTGCTCTC AGCGCAGTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229766.1
CDS	214..3036
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIASVGSREMAEAFAGEIPKVLVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDPAVRGRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAPQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFHLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKPTIQDVLRSDSQLRNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEDTKRDRSVDVNGGPEPAPASTSAVSTPSPSADLLGLGAAPPAPAGPPPSSGGSGLLVDVFSDSASVVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICSDDLQPNLNLQTKPVDPTVEGGAQVQQVVNIECVSDFTEAPVLNIQFRYGGTFQNVSVQLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEVTKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKEAVSQRLCELLSAQF

Target ORF information:

RefSeq Version	NM_001242837.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 2 subunit alpha 2 (AP2A2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242837.1

ORF Insert Sequence:

ATGCCGGCCG TGTCCAAGGG GGACGGGATG CGGGGCCTGG CGGTCTTCAT CTCGGATATC 
CGCAACTGTA AAAGTAAAGA AGCAGAAATA AAAAGGATAA ACAAGGAACT GGCAAATATC 
AGATCAAAAT TTAAAGGTGA CAAGGCTCTT GATGGCTATA GTAAAAAAAA GTACGTCTGC 
AAGTTGCTCT TCATCTTTCT CCTTGGTCAT GACATTGACT TTGGACACAT GGAGGCTGTG 
AACCTGCTGA GTTCAAACAG ATACACGGAA AAGCAGATCG GCTACCTTTT CATCTCTGTG 
TTGGTGAACT CAAACAGTGA GCTGATCCGC CTGATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGGGCCTG GCCCTGCACT GCATCGCCAG CGTGGGCAGC 
CGGGAGATGG CCGAGGCCTT CGCCGGGGAG ATCCCTAAGG TCCTCGTAGC CGGAGACACT 
ATGGACAGCG TGAAGCAGAG CGCGGCCCTG TGCTTGCTGC GCCTGTACAG GACGTCCCCC 
GATCTTGTCC CCATGGGCGA CTGGACATCC CGAGTGGTGC ACCTGCTCAA TGACCAGCAC 
TTGGGTGTGG TAACTGCAGC CACAAGTCTG ATCACCACTT TAGCACAGAA GAACCCAGAA 
GAGTTTAAAA CCTCCGTGTC TCTGGCTGTC TCTAGGCTAA GCAGAATCGT GACGTCTGCA 
TCCACAGATC TCCAGGATTA CACTTACTAT TTTGTCCCGG CTCCCTGGCT GTCTGTCAAA 
CTGCTGAGAC TGCTGCAGTG CTACCCACCC CCAGAAGACC CTGCAGTGCG AGGCCGCCTG 
ACTGAGTGCC TGGAGACCAT CCTGAACAAA GCCCAAGAAC CGCCCAAGTC GAAGAAGGTC 
CAGCACTCCA ACGCGAAGAA TGCCGTGCTC TTCGAGGCCA TCAGCTTAAT CATTCACCAT 
GACAGTGAGC CGAACCTGCT CGTCCGTGCC TGCAACCAGT TGGGCCAGTT TCTGCAGCAC 
CGCGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCTGAG 
TTCTCCCATG AGGCTGTCAA GACGCACATC GAGACGGTCA TCAACGCCCT GAAGACTGAG 
CGGGACGTGA GCGTGCGGCA GCGGGCCGTG GACCTCCTCT ACGCCATGTG CGACCGCAGC 
AACGCCCCAC AGATCGTGGC CGAGATGCTG AGCTATCTGG AGACAGCTGA CTACTCCATC 
CGAGAAGAGA TTGTGCTGAA GGTCGCCATC CTGGCTGAGA AGTACGCGGT GGACTACACC 
TGGTATGTGG ATACCATCTT GAACTTGATC CGAATTGCTG GTGATTACGT GAGTGAAGAG 
GTGTGGTACC GAGTCATTCA GATCGTCATC AACCGGGACG ACGTGCAGGG CTACGCGGCC 
AAGACTGTGT TCGAGGCTCT TCAGGCTCCC GCGTGCCACG AGAACCTGGT CAAAGTGGGC 
GGCTACATCC TGGGGGAGTT TGGAAACTTG ATAGCTGGAG ACCCGAGATC CAGCCCGCTG 
ATCCAGTTCC ACCTGCTGCA CTCCAAGTTC CACCTGTGCA GCGTCCCCAC CCGCGCGCTG 
CTCCTGTCCA CCTACATCAA GTTCGTGAAC CTCTTCCCGG AGGTGAAGCC CACCATCCAG 
GACGTGCTGC GCAGCGACAG CCAGCTCAGG AACGCAGACG TGGAGCTGCA GCAGCGTGCT 
GTGGAGTACC TGCGGCTCAG CACCGTGGCC AGCACCGACA TTCTGGCGAC CGTGCTGGAG 
GAGATGCCCC CATTCCCGGA GCGGGAGTCC TCCATCTTGG CAAAGCTCAA GAAGAAGAAG 
GGCCCCAGCA CGGTGACAGA CCTGGAGGAC ACCAAGCGGG ACAGGAGTGT GGACGTGAAC 
GGGGGTCCTG AGCCTGCCCC AGCCAGTACC AGCGCCGTGT CTACGCCTTC TCCGTCGGCA 
GACCTGCTGG GTCTCGGGGC TGCCCCCCCT GCCCCCGCGG GCCCCCCACC CTCCTCCGGC 
GGCAGCGGGC TGCTCGTGGA CGTGTTCTCA GACTCGGCCT CTGTGGTCGC GCCTCTCGCT 
CCTGGCTCCG AAGACAACTT TGCCAGGTTT GTTTGTAAAA ACAATGGTGT GTTGTTTGAA 
AACCAGCTGC TTCAAATTGG ACTTAAGTCT GAATTTCGGC AGAATTTAGG TCGGATGTTT 
ATCTTTTATG GTAATAAGAC CTCCACGCAG TTCCTAAACT TTACCCCAAC ACTAATCTGT 
TCAGACGACC TTCAGCCTAA CCTGAACCTG CAGACCAAGC CCGTGGACCC GACCGTGGAG 
GGGGGCGCGC AGGTGCAGCA GGTGGTCAAC ATAGAGTGCG TGTCCGACTT CACGGAGGCG 
CCAGTCCTCA ACATTCAGTT CAGGTATGGG GGCACCTTCC AGAACGTGTC TGTGCAGCTG 
CCCATCACTC TCAACAAATT CTTCCAGCCG ACAGAAATGG CTTCTCAGGA TTTCTTTCAA 
CGTTGGAAGC AGTTGAGCAA TCCACAGCAG GAAGTGCAGA ACATCTTCAA AGCAAAGCAC 
CCAATGGACA CAGAAGTCAC CAAAGCCAAG ATCATTGGAT TTGGTTCTGC ACTTCTTGAA 
GAAGTTGATC CTAATCCTGC GAATTTCGTG GGAGCTGGAA TCATCCACAC GAAAACCACC 
CAGATTGGAT GCCTGCTGCG CTTGGAGCCG AACCTGCAAG CCCAGATGTA CCGGCTCACG 
CTGCGCACAA GTAAGGAAGC CGTTTCTCAG AGATTATGTG AATTGCTCTC AGCGCAGTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP2A2 ( XM_011519929.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518231.1 Homo sapiens adaptor related protein complex 2 subunit alpha 2 (AP2A2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu56207
Clone ID Related Accession (Same CDS sequence)	XM_011519929.1
Accession Version	XM_011519929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	AP-2 complex subunit alpha-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG TGAACCTGCT GAGTTCAAAC AGATACACGG AAAAGCAGAT CGGCTACCTT 
TTCATCTCTG TGTTGGTGAA CTCAAACAGT GAGCTGATCC GCCTGATCAA CAACGCCATC 
AAGAATGACC TGGCCAGCCG CAACCCCACC TTCATGGGCC TGGCCCTGCA CTGCATCGCC 
AGCGTGGGCA GCCGGGAGAT GGCCGAGGCC TTCGCCGGGG AGATCCCTAA GGTCCTCGTA 
GCCGGAGACA CTATGGACAG CGTGAAGCAG AGCGCGGCCC TGTGCTTGCT GCGCCTGTAC 
AGGACGTCCC CCGATCTTGT CCCCATGGGC GACTGGACAT CCCGAGTGGT GCACCTGCTC 
AATGACCAGC ACTTGGGTGT GGTAACTGCA GCCACAAGTC TGATCACCAC TTTAGCACAG 
AAGAACCCAG AAGAGTTTAA AACCTCCGTG TCTCTGGCTG TCTCTAGGCT AAGCAGAATC 
GTGACGTCTG CATCCACAGA TCTCCAGGAT TACACTTACT ATTTTGTCCC GGCTCCCTGG 
CTGTCTGTCA AACTGCTGAG ACTGCTGCAG TGCTACCCAC CCCCAGAAGA CCCTGCAGTG 
CGAGGCCGCC TGACTGAGTG CCTGGAGACC ATCCTGAACA AAGCCCAAGA ACCGCCCAAG 
TCGAAGAAGG TCCAGCACTC CAACGCGAAG AATGCCGTGC TCTTCGAGGC CATCAGCTTA 
ATCATTCACC ATGACAGTGA GCCGAACCTG CTCGTCCGTG CCTGCAACCA GTTGGGCCAG 
TTTCTGCAGC ACCGCGAGAC CAACCTGCGC TACCTGGCCC TGGAGAGCAT GTGCACGCTG 
GCCAGCTCTG AGTTCTCCCA TGAGGCTGTC AAGACGCACA TCGAGACGGT CATCAACGCC 
CTGAAGACTG AGCGGGACGT GAGCGTGCGG CAGCGGGCCG TGGACCTCCT CTACGCCATG 
TGCGACCGCA GCAACGCCCC ACAGATCGTG GCCGAGATGC TGAGCTATCT GGAGACAGCT 
GACTACTCCA TCCGAGAAGA GATTGTGCTG AAGGTCGCCA TCCTGGCTGA GAAGTACGCG 
GTGGACTACA CCTGGTATGT GGATACCATC TTGAACTTGA TCCGAATTGC TGGTGATTAC 
GTGAGTGAAG AGGTGTGGTA CCGAGTCATT CAGATCGTCA TCAACCGGGA CGACGTGCAG 
GGCTACGCGG CCAAGACTGT GTTCGAGGCT CTTCAGGCTC CCGCGTGCCA CGAGAACCTG 
GTCAAAGTGG GCGGCTACAT CCTGGGGGAG TTTGGAAACT TGATAGCTGG AGACCCGAGA 
TCCAGCCCGC TGATCCAGTT CCACCTGCTG CACTCCAAGT TCCACCTGTG CAGCGTCCCC 
ACCCGCGCGC TGCTCCTGTC CACCTACATC AAGTTCGTGA ACCTCTTCCC GGAGGTGAAG 
CCCACCATCC AGGACGTGCT GCGCAGCGAC AGCCAGCTCA GGAACGCAGA CGTGGAGCTG 
CAGCAGCGTG CTGTGGAGTA CCTGCGGCTC AGCACCGTGG CCAGCACCGA CATTCTGGCG 
ACCGTGCTGG AGGAGATGCC CCCATTCCCG GAGCGGGAGT CCTCCATCTT GGCAAAGCTC 
AAGAAGAAGA AGGGCCCCAG CACGGTGACA GACCTGGAGG ACACCAAGCG GGACAGGAGT 
GTGGACGTGA ACGGGGGTCC TGAGCCTGCC CCAGCCAGTA CCAGCGCCGT GTCTACGCCT 
TCTCCGTCGG CAGACCTGCT GGGTCTCGGG GCTGCCCCCC CTGCCCCCGC GGGCCCCCCA 
CCCTCCTCCG GCGGCAGCGG GCTGCTCGTG GACGTGTTCT CAGACTCGGC CTCTGTGGTC 
GCGCCTCTCG CTCCTGGCTC CGAAGACAAC TTTGCCAGGT TTGTTTGTAA AAACAATGGT 
GTGTTGTTTG AAAACCAGCT GCTTCAAATT GGACTTAAGT CTGAATTTCG GCAGAATTTA 
GGTCGGATGT TTATCTTTTA TGGTAATAAG ACCTCCACGC AGTTCCTAAA CTTTACCCCA 
ACACTAATCT GTTCAGACGA CCTTCAGCCT AACCTGAACC TGCAGACCAA GCCCGTGGAC 
CCGACCGTGG AGGGGGGCGC GCAGGTGCAG CAGGTGGTCA ACATAGAGTG CGTGTCCGAC 
TTCACGGAGG CGCCAGTCCT CAACATTCAG TTCAGGTATG GGGGCACCTT CCAGAACGTG 
TCTGTGCAGC TGCCCATCAC TCTCAACAAA TTCTTCCAGC CGACAGAAAT GGCTTCTCAG 
GATTTCTTTC AACGTTGGAA GCAGTTGAGC AATCCACAGC AGGAAGTGCA GAACATCTTC 
AAAGCAAAGC ACCCAATGGA CACAGAAGTC ACCAAAGCCA AGATCATTGG ATTTGGTTCT 
GCACTTCTTG AAGAAGTTGA TCCTAATCCT GCGAATTTCG TGGGAGCTGG AATCATCCAC 
ACGAAAACCA CCCAGATTGG ATGCCTGCTG CGCTTGGAGC CGAACCTGCA AGCCCAGATG 
TACCGGCTCA CGCTGCGCAC AAGTAAGGAA GCCGTTTCTC AGAGATTATG TGAATTGCTC 
TCAGCGCAGT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518231.1
CDS	199..2793
Translation	MEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIASVGSREMAEAFAGEIPKVLVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDPAVRGRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAPQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFHLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKPTIQDVLRSDSQLRNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEDTKRDRSVDVNGGPEPAPASTSAVSTPSPSADLLGLGAAPPAPAGPPPSSGGSGLLVDVFSDSASVVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICSDDLQPNLNLQTKPVDPTVEGGAQVQQVVNIECVSDFTEAPVLNIQFRYGGTFQNVSVQLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEVTKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKEAVSQRLCELLSAQF

Target ORF information:

RefSeq Version	XM_011519929.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 2 subunit alpha 2 (AP2A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519929.1

ORF Insert Sequence:

ATGGAGGCTG TGAACCTGCT GAGTTCAAAC AGATACACGG AAAAGCAGAT CGGCTACCTT 
TTCATCTCTG TGTTGGTGAA CTCAAACAGT GAGCTGATCC GCCTGATCAA CAACGCCATC 
AAGAATGACC TGGCCAGCCG CAACCCCACC TTCATGGGCC TGGCCCTGCA CTGCATCGCC 
AGCGTGGGCA GCCGGGAGAT GGCCGAGGCC TTCGCCGGGG AGATCCCTAA GGTCCTCGTA 
GCCGGAGACA CTATGGACAG CGTGAAGCAG AGCGCGGCCC TGTGCTTGCT GCGCCTGTAC 
AGGACGTCCC CCGATCTTGT CCCCATGGGC GACTGGACAT CCCGAGTGGT GCACCTGCTC 
AATGACCAGC ACTTGGGTGT GGTAACTGCA GCCACAAGTC TGATCACCAC TTTAGCACAG 
AAGAACCCAG AAGAGTTTAA AACCTCCGTG TCTCTGGCTG TCTCTAGGCT AAGCAGAATC 
GTGACGTCTG CATCCACAGA TCTCCAGGAT TACACTTACT ATTTTGTCCC GGCTCCCTGG 
CTGTCTGTCA AACTGCTGAG ACTGCTGCAG TGCTACCCAC CCCCAGAAGA CCCTGCAGTG 
CGAGGCCGCC TGACTGAGTG CCTGGAGACC ATCCTGAACA AAGCCCAAGA ACCGCCCAAG 
TCGAAGAAGG TCCAGCACTC CAACGCGAAG AATGCCGTGC TCTTCGAGGC CATCAGCTTA 
ATCATTCACC ATGACAGTGA GCCGAACCTG CTCGTCCGTG CCTGCAACCA GTTGGGCCAG 
TTTCTGCAGC ACCGCGAGAC CAACCTGCGC TACCTGGCCC TGGAGAGCAT GTGCACGCTG 
GCCAGCTCTG AGTTCTCCCA TGAGGCTGTC AAGACGCACA TCGAGACGGT CATCAACGCC 
CTGAAGACTG AGCGGGACGT GAGCGTGCGG CAGCGGGCCG TGGACCTCCT CTACGCCATG 
TGCGACCGCA GCAACGCCCC ACAGATCGTG GCCGAGATGC TGAGCTATCT GGAGACAGCT 
GACTACTCCA TCCGAGAAGA GATTGTGCTG AAGGTCGCCA TCCTGGCTGA GAAGTACGCG 
GTGGACTACA CCTGGTATGT GGATACCATC TTGAACTTGA TCCGAATTGC TGGTGATTAC 
GTGAGTGAAG AGGTGTGGTA CCGAGTCATT CAGATCGTCA TCAACCGGGA CGACGTGCAG 
GGCTACGCGG CCAAGACTGT GTTCGAGGCT CTTCAGGCTC CCGCGTGCCA CGAGAACCTG 
GTCAAAGTGG GCGGCTACAT CCTGGGGGAG TTTGGAAACT TGATAGCTGG AGACCCGAGA 
TCCAGCCCGC TGATCCAGTT CCACCTGCTG CACTCCAAGT TCCACCTGTG CAGCGTCCCC 
ACCCGCGCGC TGCTCCTGTC CACCTACATC AAGTTCGTGA ACCTCTTCCC GGAGGTGAAG 
CCCACCATCC AGGACGTGCT GCGCAGCGAC AGCCAGCTCA GGAACGCAGA CGTGGAGCTG 
CAGCAGCGTG CTGTGGAGTA CCTGCGGCTC AGCACCGTGG CCAGCACCGA CATTCTGGCG 
ACCGTGCTGG AGGAGATGCC CCCATTCCCG GAGCGGGAGT CCTCCATCTT GGCAAAGCTC 
AAGAAGAAGA AGGGCCCCAG CACGGTGACA GACCTGGAGG ACACCAAGCG GGACAGGAGT 
GTGGACGTGA ACGGGGGTCC TGAGCCTGCC CCAGCCAGTA CCAGCGCCGT GTCTACGCCT 
TCTCCGTCGG CAGACCTGCT GGGTCTCGGG GCTGCCCCCC CTGCCCCCGC GGGCCCCCCA 
CCCTCCTCCG GCGGCAGCGG GCTGCTCGTG GACGTGTTCT CAGACTCGGC CTCTGTGGTC 
GCGCCTCTCG CTCCTGGCTC CGAAGACAAC TTTGCCAGGT TTGTTTGTAA AAACAATGGT 
GTGTTGTTTG AAAACCAGCT GCTTCAAATT GGACTTAAGT CTGAATTTCG GCAGAATTTA 
GGTCGGATGT TTATCTTTTA TGGTAATAAG ACCTCCACGC AGTTCCTAAA CTTTACCCCA 
ACACTAATCT GTTCAGACGA CCTTCAGCCT AACCTGAACC TGCAGACCAA GCCCGTGGAC 
CCGACCGTGG AGGGGGGCGC GCAGGTGCAG CAGGTGGTCA ACATAGAGTG CGTGTCCGAC 
TTCACGGAGG CGCCAGTCCT CAACATTCAG TTCAGGTATG GGGGCACCTT CCAGAACGTG 
TCTGTGCAGC TGCCCATCAC TCTCAACAAA TTCTTCCAGC CGACAGAAAT GGCTTCTCAG 
GATTTCTTTC AACGTTGGAA GCAGTTGAGC AATCCACAGC AGGAAGTGCA GAACATCTTC 
AAAGCAAAGC ACCCAATGGA CACAGAAGTC ACCAAAGCCA AGATCATTGG ATTTGGTTCT 
GCACTTCTTG AAGAAGTTGA TCCTAATCCT GCGAATTTCG TGGGAGCTGG AATCATCCAC 
ACGAAAACCA CCCAGATTGG ATGCCTGCTG CGCTTGGAGC CGAACCTGCA AGCCCAGATG 
TACCGGCTCA CGCTGCGCAC AAGTAAGGAA GCCGTTTCTC AGAGATTATG TGAATTGCTC 
TCAGCGCAGT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of clathrin-mediated endocytosis by hierarchical allosteric activation of AP2.
The Journal of cell biology216(1)167-179(2017 Jan)
Kadlecova Z,Spielman SJ,Loerke D,Mohanakrishnan A,Reed DK,Schmid SL

CALM regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature.
Developmental cell33(2)163-75(2015 Apr)
Miller SE,Mathiasen S,Bright NA,Pierre F,Kelly BT,Kladt N,Schauss A,Merrifield CJ,Stamou D,H?ning S,Owen DJ

Genetic susceptibility for chronic bronchitis in chronic obstructive pulmonary disease.
Respiratory research15113(2014 Sep)
Lee JH,Cho MH,Hersh CP,McDonald ML,Crapo JD,Bakke PS,Gulsvik A,Comellas AP,Wendt CH,Lomas DA,Kim V,Silverman EK,

Meta-analysis of 74,046 individuals identifies 11 new susceptibility loci for Alzheimer's disease.
Nature genetics45(12)1452-8(2013 Dec)
Lambert JC,Ibrahim-Verbaas CA,Harold D,Naj AC,Sims R,Bellenguez C,DeStafano AL,Bis JC,Beecham GW,Grenier-Boley B,Russo G,Thorton-Wells TA,Jones N,Smith AV,Chouraki V,Thomas C,Ikram MA,Zelenika D,Vardarajan BN,Kamatani Y,Lin CF,Gerrish A,Schmidt H,Kunkle B,Dunstan ML,Ruiz A,Bihoreau MT,Choi SH,Reitz C,Pasquier F,Cruchaga C,Craig D,Amin N,Berr C,Lopez OL,De Jager PL,Deramecourt V,Johnston JA,Evans D,Lovestone S,Letenneur L,Mor?n FJ,Rubinsztein DC,Eiriksdottir G,Sleegers K,Goate AM,Fi?vet N,Huentelman MW,Gill M,Brown K,Kamboh MI,Keller L,Barberger-Gateau P,McGuiness B,Larson EB,Green R,Myers AJ,Dufouil C,Todd S,Wallon D,Love S,Rogaeva E,Gallacher J,St George-Hyslop P,Clarimon J,Lleo A,Bayer A,Tsuang DW,Yu L,Tsolaki M,Boss? P,Spalletta G,Proitsi P,Collinge J,Sorbi S,Sanchez-Garcia F,Fox NC,Hardy J,Deniz Naranjo MC,Bosco P,Clarke R,Brayne C,Galimberti D,Mancuso M,Matthews F,,,,,Moebus S,Mecocci P,Del Zompo M,Maier W,Hampel H,Pilotto A,Bullido M,Panza F,Caffarra P,Nacmias B,Gilbert JR,Mayhaus M,Lannefelt L,Hakonarson H,Pichler S,Carrasquillo MM,Ingelsson M,Beekly D,Alvarez V,Zou F,Valladares O,Younkin SG,Coto E,Hamilton-Nelson KL,Gu W,Razquin C,Pastor P,Mateo I,Owen MJ,Faber KM,Jonsson PV,Combarros O,O'Donovan MC,Cantwell LB,Soininen H,Blacker D,Mead S,Mosley TH Jr,Bennett DA,Harris TB,Fratiglioni L,Holmes C,de Bruijn RF,Passmore P,Montine TJ,Bettens K,Rotter JI,Brice A,Morgan K,Foroud TM,Kukull WA,Hannequin D,Powell JF,Nalls MA,Ritchie K,Lunetta KL,Kauwe JS,Boerwinkle E,Riemenschneider M,Boada M,Hiltuenen M,Martin ER,Schmidt R,Rujescu D,Wang LS,Dartigues JF,Mayeux R,Tzourio C,Hofman A,N?then MM,Graff C,Psaty BM,Jones L,Haines JL,Holmans PA,Lathrop M,Pericak-Vance MA,Launer LJ,Farrer LA,van Duijn CM,Van Broeckhoven C,Moskvina V,Seshadri S,Williams J,Schellenberg GD,Amouyel P

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AP2A2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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