Numa1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006507171.3	XP_006507234.1	nuclear mitotic apparatus protein 1 isoform X1
XM_006507177.2	XP_006507240.1	nuclear mitotic apparatus protein 1 isoform X2
XM_006507174.2	XP_006507237.1	nuclear mitotic apparatus protein 1 isoform X1
XM_030241948.1	XP_030097808.1	nuclear mitotic apparatus protein 1 isoform X1
XM_030241949.1	XP_030097809.1	nuclear mitotic apparatus protein 1 isoform X3
XM_006507175.2	XP_006507238.1	nuclear mitotic apparatus protein 1 isoform X1
XM_006507171.4	XP_006507234.1	nuclear mitotic apparatus protein 1 isoform X1
XM_006507173.3	XP_006507236.1	nuclear mitotic apparatus protein 1 isoform X1
XM_006507175.3	XP_006507238.1	nuclear mitotic apparatus protein 1 isoform X1
XM_006507177.3	XP_006507240.1	nuclear mitotic apparatus protein 1 isoform X2
XM_030241950.1	XP_030097810.1	nuclear mitotic apparatus protein 1 isoform X4
XM_006507173.2	XP_006507236.1	nuclear mitotic apparatus protein 1 isoform X1
XM_006507172.3	XP_006507235.1	nuclear mitotic apparatus protein 1 isoform X1
XM_006507178.2	XP_006507241.1	nuclear mitotic apparatus protein 1 isoform X3
XM_006507169.3	XP_006507232.1	nuclear mitotic apparatus protein 1 isoform X1
XM_006507170.3	XP_006507233.1	nuclear mitotic apparatus protein 1 isoform X1
XM_006507172.4	XP_006507235.1	nuclear mitotic apparatus protein 1 isoform X1
XM_006507169.2	XP_006507232.1	nuclear mitotic apparatus protein 1 isoform X1
XM_006507170.2	XP_006507233.1	nuclear mitotic apparatus protein 1 isoform X1
XM_006507174.1	XP_006507237.1	nuclear mitotic apparatus protein 1 isoform X1
NM_133947.3	NP_598708.3	nuclear mitotic apparatus protein 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13199	XM_006507177.3 Latest version!	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu39199	XM_006507169.3 Latest version!	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu39199	XM_006507172.4 Latest version!	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu39199	XM_006507173.3 Latest version!	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu39199	XM_006507174.2 Latest version!	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu39199	XM_006507171.4 Latest version!	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu39199	XM_006507175.3 Latest version!	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu39199	XM_030241948.1 Latest version!	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu39199	XM_006507170.3 Latest version!	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu39201	XM_030241949.1 Latest version!	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu89308	XM_030241950.1 Latest version!	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu13199	NM_133947.3 Latest version!	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu39201	XM_006507178.2 Latest version!	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu39199	XM_006507171.3	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu39199	XM_006507175.2	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu13199	XM_006507177.2	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu39199	XM_006507173.2	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu39199	XM_006507172.3	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu39199	XM_006507169.2	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu39199	XM_006507170.2	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu39199	XM_006507174.1	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

Numa1 ( XM_006507177.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507240.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu13199
Clone ID Related Accession (Same CDS sequence)	XM_006507177.2 , XM_006507177.3 , NM_133947.3
Accession Version	XM_006507177.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507177.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AAGAACTAAG TAAGAAGCTG 
ACAGAGCATG ACCAAGCCAG CAAGGTGCAG CAACAGAAGC TAAAGGCTTT CCAGGCCCAG 
AGAGGTGAAA GCCAGCAAGA GGTCCAGCGC CTCCAGACCC AGCTGAACGA GCTGCAGGCC 
CAGCTGAGCC AGAAGGAGCA GGCAGCTGAG CATTACAAGC TCCAGATGGA GAAAGCCAAG 
ACCCATTATG ATGCCAAGAA GCAGCAGAAC CAAAAGCTGC AGGAACAGCT TCAAGACTTG 
GAGGAACTGC AGAAGGAGAA CAAGGAGCTG CGGTCTGAAG CTGAACGTCT GGGCCGTGAG 
CTGCAGCAGG CAGGGCTGAA GACCAAGGAA GCTGAACAGA CCTGCCGTCA CCTTACAGCC 
CAGGTGCGCA GCCTGGAGGC CCAGGTTGCC CATGCAGACC AGCAGCTTCG TGACTTGGGC 
AAATTCCAGG TGGCAACTGA TGCCTTAAAG AGCCGTGAGC CTCAAGTGAA ACCCCAGCTG 
GACTTAAGTA TTGACAGCCT GGATCTGAGT TTGGAGGAGG GAACCCCATG CAGTGTGGCT 
AGCAAGCTGC CTCGTACCCA ACCAGATGGT ACCAGTGTCC CTGGAGAGCC AGCTTCACCT 
ATCTCCCAGC GCCTGCCCCC CAAAGTGGAA TCCCTGGAGA GCCTCTACTT CACCCCCACT 
CCTGCTCGGG GTCAGGCCCC CTTGGAGACT AGCCTTGACT CTCTGGGGGA TGCCTTCCCT 
GACTCCGGCC GTAAGACCCG CTCTGCTCGC CGCCGTACCA CACAAATCAT CAACATCACT 
ATGACCAAGA AATTAGAATT GGAAGAGCCA GACAGCGCCA ACTCATCCTT CTACAGCACG 
CAGTCTGCTC CTGCTTCCCA GGCCAACTTG CGAGCCACTT CCTCCACTCA GTCTCTGGCC 
CGCTTGGGTT CTCCTGATGA TGGCAACTCC GCTCTGCTTA GCCTGCCTGG CTACCGGCCT 
ACCACACGAA GCTCTGCTCG TCGCTCCCAG GCCAGGATGT CCAGTGGGGC CCCCCAAGGG 
AGGAATAGCT TCTACATGGG CACTTGCCAG GATGAGCCCG AGCAGCTGGA TGATTGGAAC 
CGCATCGCAG AGTTGCAGCA GCGCAACCGA GTATGCCCCC CGCACCTGAA GACCTGCTAC 
CCTCTGGAGT CCAGGCCTAC CCTGAGCCTG GCCACCATCA CTGATGAGGA GATGAAGACT 
GGAGACCCCA GGGAAACCTT GCGTAGAGCC AGCATGCAGC CAGCTCAGAT TGCTGAGGGT 
GTTGGCATAA CTACCAGGCA GCAGCGCAAA CGAGTTTCCT CTGAGACCCA CCAGGGCCCT 
GGCACTCCTG AGTCCAAGAA AGCTACCAGC TGCTTTCCAC GCCCCATGAC CCCCAGGGAC 
CGGCATGAAG GACGTAAGCA GAGCAGCACT GCTGACACCC AGAAGAAAGC AGCTCCAGTT 
CTCAAACAGG CGGACCGGCG TCAGTCAATG GCTTTCAGCA TCCTTAACAC ACCTAAGAAG 
CTGGGAAATA GTCTTCTAAG GCGAGGAGCT TCAAAGAAGA CCCCAGCAAA GGTCTCCCCC 
AATCCTCGCA GTGGAACCCG CCGTTCTCCA CGCATTGCCA CCACCACAAC TGGCACTGCT 
ACTGTTGCCA CCACTCCTCG GGCCAAGGGC AAGGTGAAGC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507240.1
CDS	229..6513
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507177.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507177.3

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AAGAACTAAG TAAGAAGCTG 
ACAGAGCATG ACCAAGCCAG CAAGGTGCAG CAACAGAAGC TAAAGGCTTT CCAGGCCCAG 
AGAGGTGAAA GCCAGCAAGA GGTCCAGCGC CTCCAGACCC AGCTGAACGA GCTGCAGGCC 
CAGCTGAGCC AGAAGGAGCA GGCAGCTGAG CATTACAAGC TCCAGATGGA GAAAGCCAAG 
ACCCATTATG ATGCCAAGAA GCAGCAGAAC CAAAAGCTGC AGGAACAGCT TCAAGACTTG 
GAGGAACTGC AGAAGGAGAA CAAGGAGCTG CGGTCTGAAG CTGAACGTCT GGGCCGTGAG 
CTGCAGCAGG CAGGGCTGAA GACCAAGGAA GCTGAACAGA CCTGCCGTCA CCTTACAGCC 
CAGGTGCGCA GCCTGGAGGC CCAGGTTGCC CATGCAGACC AGCAGCTTCG TGACTTGGGC 
AAATTCCAGG TGGCAACTGA TGCCTTAAAG AGCCGTGAGC CTCAAGTGAA ACCCCAGCTG 
GACTTAAGTA TTGACAGCCT GGATCTGAGT TTGGAGGAGG GAACCCCATG CAGTGTGGCT 
AGCAAGCTGC CTCGTACCCA ACCAGATGGT ACCAGTGTCC CTGGAGAGCC AGCTTCACCT 
ATCTCCCAGC GCCTGCCCCC CAAAGTGGAA TCCCTGGAGA GCCTCTACTT CACCCCCACT 
CCTGCTCGGG GTCAGGCCCC CTTGGAGACT AGCCTTGACT CTCTGGGGGA TGCCTTCCCT 
GACTCCGGCC GTAAGACCCG CTCTGCTCGC CGCCGTACCA CACAAATCAT CAACATCACT 
ATGACCAAGA AATTAGAATT GGAAGAGCCA GACAGCGCCA ACTCATCCTT CTACAGCACG 
CAGTCTGCTC CTGCTTCCCA GGCCAACTTG CGAGCCACTT CCTCCACTCA GTCTCTGGCC 
CGCTTGGGTT CTCCTGATGA TGGCAACTCC GCTCTGCTTA GCCTGCCTGG CTACCGGCCT 
ACCACACGAA GCTCTGCTCG TCGCTCCCAG GCCAGGATGT CCAGTGGGGC CCCCCAAGGG 
AGGAATAGCT TCTACATGGG CACTTGCCAG GATGAGCCCG AGCAGCTGGA TGATTGGAAC 
CGCATCGCAG AGTTGCAGCA GCGCAACCGA GTATGCCCCC CGCACCTGAA GACCTGCTAC 
CCTCTGGAGT CCAGGCCTAC CCTGAGCCTG GCCACCATCA CTGATGAGGA GATGAAGACT 
GGAGACCCCA GGGAAACCTT GCGTAGAGCC AGCATGCAGC CAGCTCAGAT TGCTGAGGGT 
GTTGGCATAA CTACCAGGCA GCAGCGCAAA CGAGTTTCCT CTGAGACCCA CCAGGGCCCT 
GGCACTCCTG AGTCCAAGAA AGCTACCAGC TGCTTTCCAC GCCCCATGAC CCCCAGGGAC 
CGGCATGAAG GACGTAAGCA GAGCAGCACT GCTGACACCC AGAAGAAAGC AGCTCCAGTT 
CTCAAACAGG CGGACCGGCG TCAGTCAATG GCTTTCAGCA TCCTTAACAC ACCTAAGAAG 
CTGGGAAATA GTCTTCTAAG GCGAGGAGCT TCAAAGAAGA CCCCAGCAAA GGTCTCCCCC 
AATCCTCGCA GTGGAACCCG CCGTTCTCCA CGCATTGCCA CCACCACAAC TGGCACTGCT 
ACTGTTGCCA CCACTCCTCG GGCCAAGGGC AAGGTGAAGC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_006507174.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507237.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X6, mRNA.

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CloneID	OMu39199
Clone ID Related Accession (Same CDS sequence)	XM_006507171.3 , XM_006507174.2 , XM_030241948.1 , XM_006507175.2 , XM_006507171.4 , XM_006507173.3 , XM_006507175.3 , XM_006507173.2 , XM_006507172.3 , XM_006507169.3 , XM_006507170.3 , XM_006507172.4 , XM_006507169.2 , XM_006507170.2 , XM_006507174.1
Accession Version	XM_006507174.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507237.1
CDS	176..6502
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507174.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507174.2

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_030241948.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097808.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X8, mRNA.

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CloneID	OMu39199
Clone ID Related Accession (Same CDS sequence)	XM_006507171.3 , XM_006507174.2 , XM_030241948.1 , XM_006507175.2 , XM_006507171.4 , XM_006507173.3 , XM_006507175.3 , XM_006507173.2 , XM_006507172.3 , XM_006507169.3 , XM_006507170.3 , XM_006507172.4 , XM_006507169.2 , XM_006507170.2 , XM_006507174.1
Accession Version	XM_030241948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097808.1
CDS	249..6575
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_030241948.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241948.1

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_006507171.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507234.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X3, mRNA.

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CloneID	OMu39199
Clone ID Related Accession (Same CDS sequence)	XM_006507171.3 , XM_006507174.2 , XM_030241948.1 , XM_006507175.2 , XM_006507171.4 , XM_006507173.3 , XM_006507175.3 , XM_006507173.2 , XM_006507172.3 , XM_006507169.3 , XM_006507170.3 , XM_006507172.4 , XM_006507169.2 , XM_006507170.2 , XM_006507174.1
Accession Version	XM_006507171.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507171.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507234.1
CDS	203..6529
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507171.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507171.4

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_006507173.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507236.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X5, mRNA.

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CloneID	OMu39199
Clone ID Related Accession (Same CDS sequence)	XM_006507171.3 , XM_006507174.2 , XM_030241948.1 , XM_006507175.2 , XM_006507171.4 , XM_006507173.3 , XM_006507175.3 , XM_006507173.2 , XM_006507172.3 , XM_006507169.3 , XM_006507170.3 , XM_006507172.4 , XM_006507169.2 , XM_006507170.2 , XM_006507174.1
Accession Version	XM_006507173.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507173.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507236.1
CDS	345..6671
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507173.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507173.3

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_006507175.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507238.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X7, mRNA.

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CloneID	OMu39199
Clone ID Related Accession (Same CDS sequence)	XM_006507171.3 , XM_006507174.2 , XM_030241948.1 , XM_006507175.2 , XM_006507171.4 , XM_006507173.3 , XM_006507175.3 , XM_006507173.2 , XM_006507172.3 , XM_006507169.3 , XM_006507170.3 , XM_006507172.4 , XM_006507169.2 , XM_006507170.2 , XM_006507174.1
Accession Version	XM_006507175.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507175.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507238.1
CDS	288..6614
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507175.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507175.3

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_006507169.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507232.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X1, mRNA.

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CloneID	OMu39199
Clone ID Related Accession (Same CDS sequence)	XM_006507171.3 , XM_006507174.2 , XM_030241948.1 , XM_006507175.2 , XM_006507171.4 , XM_006507173.3 , XM_006507175.3 , XM_006507173.2 , XM_006507172.3 , XM_006507169.3 , XM_006507170.3 , XM_006507172.4 , XM_006507169.2 , XM_006507170.2 , XM_006507174.1
Accession Version	XM_006507169.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507169.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507232.1
CDS	229..6555
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507169.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507169.3

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_006507170.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507233.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X2, mRNA.

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CloneID	OMu39199
Clone ID Related Accession (Same CDS sequence)	XM_006507171.3 , XM_006507174.2 , XM_030241948.1 , XM_006507175.2 , XM_006507171.4 , XM_006507173.3 , XM_006507175.3 , XM_006507173.2 , XM_006507172.3 , XM_006507169.3 , XM_006507170.3 , XM_006507172.4 , XM_006507169.2 , XM_006507170.2 , XM_006507174.1
Accession Version	XM_006507170.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507170.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507233.1
CDS	315..6641
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507170.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507170.3

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_006507172.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507235.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X4, mRNA.

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CloneID	OMu39199
Clone ID Related Accession (Same CDS sequence)	XM_006507171.3 , XM_006507174.2 , XM_030241948.1 , XM_006507175.2 , XM_006507171.4 , XM_006507173.3 , XM_006507175.3 , XM_006507173.2 , XM_006507172.3 , XM_006507169.3 , XM_006507170.3 , XM_006507172.4 , XM_006507169.2 , XM_006507170.2 , XM_006507174.1
Accession Version	XM_006507172.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507172.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507235.1
CDS	394..6720
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507172.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507172.4

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_030241949.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097809.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X10, mRNA.

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CloneID	OMu39201
Clone ID Related Accession (Same CDS sequence)	XM_030241949.1 , XM_006507178.2
Accession Version	XM_030241949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCAT TGGGTGGCCC CAAAGCCGAG CTGACTTCAG ATGGGAATAG AAAGAAGGCA 
GAAAATCGGA AACATCCTTC ATCTACACAA TGCCTGGTAT CTGTGCAGAA AGTGATAGAG 
GGATCCGAGA TGGAACTGGC CAAGATGATA ATGCTGTTTT TATATCAGTC CACCATGAGC 
TCCAGAAACC TCCGGGACTG GGAGCAGTTT GAATATGGGG TTCAGGCTGA GTTAGCGGTC 
ATCCTGAAGT TTATGCTGGA TCATGAGGAG TCGCTAAACC TTACTGAGGA CTTAGAGAGT 
TTCTTGGAGA AAGTTCCTTA CACCCATGCC AGCACCCTCT CAGAAGAGCT TTCTCCACCC 
AGCCACCAGA CCAAGAGGAA GATTCGCTTC CTAGAAATAC AGAGGATTGC TTCATCTTCC 
AGTGAGAACA ACTTCCTCTC AGGCTCTCCA TCCTCCCCTA TGGGCGACAT ACTGCAGACC 
CCACAGTTCC AGATGAGACG ACTGAAGAAG CAGCTGGCAG ACGAGAGGAG CAACAGAGAT 
GACCTGGAGC TGGAGCTGTC TGAGAGCCTC AAGCTCCTCA CTGAGAAGGA CGCGCAGATC 
GCCATGATGC AGCAGCGCAT TGATCACCTG GCTCTGCTGA ATGAGAAGCA GGCAGCCAGC 
TCCCAGGAGC CCAGTGAGCT TGAGGAGCTC CGAGGCAAGA ATGAAAGCCT CACTGTGCGG 
CTGCATGAGA CTTTGAAGCA GTGTCAGAAC CTGAAGACCG AGAAGAGCCA GATGGATCGA 
AAGATTAGCC AGCTTTCTGA GGAGAATGGG GACCTTTCCT TTAAGGTTCG AGAGTTTGCA 
AACCACTTGC AACAACTTCA GGGTGCATTC AACGACCTGA TAGAGGAGCA CAGCAAGGCT 
TCTCAGGAGT GGGCAGAGAA GCAGGCTCGT CTGGAAAATG AGCTCAGCAC AGCCCTCCAG 
GATAAGAAAT GTCTTGAAGA GAAGAATGAA ATTCTTCAGG GAAAGCTTTC CCAGCTGGAA 
GATCAAGCAA CTCGGCTGCA GGAGAGCCCA GCCCCGGAGA AGGGTGAGGT GCTAGGAGAC 
GCCTTGCAGC TGGACACTCT AAAGCAAGAG GCAGCCAAAC TTGCCACAGA CAACACCCAG 
CTCCAAACCC GCGTAGAGAC ACTGGAGTGT GAACGTGGCA AGCAGGAAGC CCAGCTGCTT 
GCTGAACGGA GCCGCTTTGA AGACGAAAAG CAGCAGCTGG CCAGCTTGAT TGCTGACCTT 
CAGAGCTCCG TCTCTAACCT CAGCCAGGCC AAAGAGGAGT TAGAGCAAGC CTCCCAGGCC 
CAAGGGGCTC AGCTCACTGC CCAGTTGACC TCTATGACAG GGCTCAATGC CACCCTGCAG 
CAGCGAGATC AAGAGCTGGC CAGTCTGAAA GAACAGGCCA AAAAGGAACA GGCTCAGATG 
CTACAGACCA TGCAAGAGCA AGAACAGGCT GCCCAGGGCC TCCGCCAGCA GGTGGAGCAG 
CTGAGCAGCA GCCTGAAGCT GAAGGAGCAG CAATTAGAAG AGGCAGCCAA GGAGCAGGAG 
GCAACCAGAC AGGATCACGC CCAGCAACTG GCCATCGTTG CTGAGGCTCG AGAGGCCTCT 
CTAAGGGAAC GGGATACTGC TCGCCAGCAG CTAGAAACAG TGGAGAAGGA GAAGGATGCC 
AAGCTAGAGA GTCTGCAGCA GCAACTTCAG GCTGCCAATG ATGCTAGGGA CAATGCGCAG 
ACCTCGGTCA CACAGGCTCA GCAGGAAAAG GCAGAGCTGA GCCAAAAGAT AGGAGAGCTT 
CATGCCTGCA TTGAAGCTTC CCACCAGGAA CAGCGTCAGG TTCAAGCTCG TGTGACAGAG 
CTAGAAGCCC AGCTGAAAGC TGAGCAGCAA AAAACAACTG AGCGAGAAAA GGTGGTTCAG 
GAGAAGGCCC AGCTCCAGGA GCAGCTCCGG GCCCTTGAGG AGTCTTTGAA GATTACCAAA 
GGCAGCCTTG AAGAAGAGAA ACGCAGGGCT GCAGATGCCC TGAAGGAGCA GCAGTGCCGT 
GCCACTGAGA TGGAGGCAGA GTCTCGAAGC CTGATGGAGC AGCGGGAGCG GGAACAGAAG 
GAGTTAGAGC AAGAGAAGGC TGGGCGAAAG GGGCTGGAGG CCCGGATACA GCAGCTTGAA 
GAGGCTCATC AGGCTGAGAC TGAAGCCTTG CGGCATGAGC TGGCAGAGGC CACAGCTTCC 
CAGCACAGAG CTGAGAGTGA GTGTGAGCGG CTCATCAGAG AGGTTGAGAG CCGGCAGAAG 
CGGTTTGAAG CTAGACAGCA AGAGGAGGCA AGGTACGGTG CCATGTTCCA GGAACAGCTG 
ATGGCTTTGA AAGGGGAGAA GACTGGCCAA GAGGTCCAGG AGGAGGCAGT AGAGATCCAC 
AGTGAAGGCC AGCCAGGCCA GCAGCAGAGT CAGCTAGCCC AGCTTCATGC CAGTCTGGCC 
AAAGCCATCC AGCAGGTCCA GGAGAAGGAA GTGAGAGCAC AGAAGCTGGT AGATGATCTC 
TCAGCTCTGC AGGAGAAGAT GGCTGCCACT AACAAGGAGG TGGCCTGTCT GAAGACCTTG 
GTGCTCAAGG CAGGTGAGCA GCAGGAAACA GCCTCACTTG AGCTGCTCAA AGAGCCCCCG 
AGAGCTGCAA ACAGAGCGTC TGACCAGCTA GGAGAACAGC AGGGACGGCC ATTCTCTAGT 
ACACACGCAG CAGTAAAAGC TATGGAGCGT GAAGCTGAGC AGATGGGCGG TGAACTGGAG 
AGGCTGCGGG CAGCACTGAT CAAGAGCCAG GGCCAGCAGC AAGAGGAGCG TGGGCAACAG 
GAGAGGGAGG TAGCACGGCT GACCCAGGAG CGGGGCCAGG CCCAGGCTGA CCTGGCTCAG 
GAGAAGGCAG CCAAGGCAGA GCTGGAGATG CGACTGCAGA ACACCCTCAA TGAGCAGCGT 
GTGGAGTTTG CTGCCCTACA GGAAGCACTG GCCCATGCCC TGACAGAAAA GGAAGGGACA 
GACCAAGAGC TTGCCAAACT TCGAGGGCAG GAGGCAGCTC AAAGAACAGA GCTGAAGGAG 
CTTCAACAGA CTTTGGAACA GCTGAAAATA CAGTTAGTGA AGAAAGAAAA AGAGCACCCT 
GCAGGGGGTG CCAGTGGAGA AGATGCTTCT GGCCCAGGAA CCCAGTCGGA GACAGCTGGA 
AAGACAGATG CACCAGGCCC TGAGTTGCAG GCACTTCGGG CAGAGATCAG TAAGCTAGAG 
CAGCAGTGTC AGCAGCAGCA GCAACAGGTG GAAGGCCTGA CACACAGCCT CAAGTCTGAG 
CGGGCTTGCC GGGCCGAGCA AGACAAAGCC CTGGAGACAC TGCAGGGCCA GTTAGAAGAG 
AAGGCTCGAG AGCTAGGGCA TAATCAGGCT GCCTCAGCTT CAGCTCAGAG AGAGTTGCAA 
GCCCTCCGTG CTAAAGCCCA GGACCATAGC AAAGCTGAGG AAGAGTGGAA GGCCCAGGTA 
GCCCGGGGCC AGCAGGAGGC TGAAAGAAAA AGCAGTCTTA TCAGCAGCTT GGAAGAGGAG 
GTATCCATCC TGAATCGCCA AGTCCTAGAG AAGGAAGGGG AGAGCAAGGA GTTGAAGCGC 
CTGGTTGTAG CTGAATCCGA GAAGAGCCAG AAGCTGGAAG AGAGGCTGCG CCTGCTTCAA 
GTAGAAACAG CCAGCAATAG TGCCAGGGCA GCAGAACGCA GCTCAGCTCT ACGAGAGGAG 
GTCCAGAGCC TCCGGGAAGA GGTGGAGAAA CAGCGTGTAG TTTCAGAGAA CTCGCGGCAG 
GAGTTGGCCT CACAAGCAGA GCGAGCTGAG GAGTTGGGCC AAGAATTGAA GGCCTGGCAG 
GAGAAGTTTT TCCAGAAGGA GCAAGCTCTC TCTGCCCTGC AGCTGGAGCA CACCAGCACA 
CAGGCCCTGG TAAGTGAGCT GCTGCCTGCC AAGCACCTTT GTCAGCAGCT GCAAGCTGAG 
CAAGCGGCTG CCGAGAAGCG CTTCCGGGAG GAGCTCGAGC AGAGCAAGCA GGCAGCTGGT 
GGGCTCCAGG CAGAGCTGAT GCGGGCCCAG CGGGAGCTCG GAGAGCTCGG ATCTTTGCGG 
CAGAAGATAG TTGAGCAGGA GCGAGCAGCC CAGCAGCTGC GGGCAGAGAA GGCCAGCTAT 
GCAGAGCAGC TGAGCATGCT GAAGAAGGCC CATGGCCTGC TGGCAGAGGA GAACCGGGGG 
CTGGGAGAGC GGGCCAACCT TGGCCGGCAG TTTCTGGAAG TAGAACTAGA TCAAGCCCGG 
GAGAAATATG TCCAGGAGTT GGCTGCTGTG CGTACTGATG CTGAGACCCA CTTGGCTGAA 
ATGCGACAAG AAGCACAGAG TACTAGCCGG GAGCTGGAGG TGATGACTGC CAAGTACGAA 
GGTGCCAAGG TGAAGGTCCT AGAGGAAAGG CAGAGGTTCC AAGAAGAGAG GCAGAAACTC 
ACTGCCCAGG TGGAGCAACT AGAGGTATTT CAAAGAGAGC AGACTAAGCA GGTGGAAGAA 
CTAAGTAAGA AGCTGACAGA GCATGACCAA GCCAGCAAGG TGCAGCAACA GAAGCTAAAG 
GCTTTCCAGG CCCAGAGAGG TGAAAGCCAG CAAGAGGTCC AGCGCCTCCA GACCCAGCTG 
AACGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCAG CTGAGCATTA CAAGCTCCAG 
ATGGAGAAAG CCAAGACCCA TTATGATGCC AAGAAGCAGC AGAACCAAAA GCTGCAGGAA 
CAGCTTCAAG ACTTGGAGGA ACTGCAGAAG GAGAACAAGG AGCTGCGGTC TGAAGCTGAA 
CGTCTGGGCC GTGAGCTGCA GCAGGCAGGG CTGAAGACCA AGGAAGCTGA ACAGACCTGC 
CGTCACCTTA CAGCCCAGGT GCGCAGCCTG GAGGCCCAGG TTGCCCATGC AGACCAGCAG 
CTTCGTGACT TGGGCAAATT CCAGGTGGCA ACTGATGCCT TAAAGAGCCG TGAGCCTCAA 
GTGAAACCCC AGCTGGACTT AAGTATTGAC AGCCTGGATC TGAGTTTGGA GGAGGGAACC 
CCATGCAGTG TGGCTAGCAA GCTGCCTCGT ACCCAACCAG ATGGTACCAG TGTCCCTGGA 
GAGCCAGCTT CACCTATCTC CCAGCGCCTG CCCCCCAAAG TGGAATCCCT GGAGAGCCTC 
TACTTCACCC CCACTCCTGC TCGGGGTCAG GCCCCCTTGG AGACTAGCCT TGACTCTCTG 
GGGGATGCCT TCCCTGACTC CGGCCGTAAG ACCCGCTCTG CTCGCCGCCG TACCACACAA 
ATCATCAACA TCACTATGAC CAAGAAATTA GAATTGGAAG AGCCAGACAG CGCCAACTCA 
TCCTTCTACA GCACGCAGTC TGCTCCTGCT TCCCAGGCCA ACTTGCGAGC CACTTCCTCC 
ACTCAGTCTC TGGCCCGCTT GGGTTCTCCT GATGATGGCA ACTCCGCTCT GCTTAGCCTG 
CCTGGCTACC GGCCTACCAC ACGAAGCTCT GCTCGTCGCT CCCAGGCCAG GATGTCCAGT 
GGGGCCCCCC AAGGGAGGAA TAGCTTCTAC ATGGGCACTT GCCAGGATGA GCCCGAGCAG 
CTGGATGATT GGAACCGCAT CGCAGAGTTG CAGCAGCGCA ACCGAGTATG CCCCCCGCAC 
CTGAAGACCT GCTACCCTCT GGAGTCCAGG CCTACCCTGA GCCTGGCCAC CATCACTGAT 
GAGGAGATGA AGACTGGAGA CCCCAGGGAA ACCTTGCGTA GAGCCAGCAT GCAGCCAGCT 
CAGATTGCTG AGGGTGTTGG CATAACTACC AGGCAGCAGC GCAAACGAGT TTCCTCTGAG 
ACCCACCAGG GCCCTGGCAC TCCTGAGTCC AAGAAAGCTA CCAGCTGCTT TCCACGCCCC 
ATGACCCCCA GGGACCGGCA TGAAGGACGT AAGCAGAGCA GCACTGCTGA CACCCAGAAG 
AAAGCAGCTC CAGTTCTCAA ACAGGCGGAC CGGCGTCAGT CAATGGCTTT CAGCATCCTT 
AACACACCTA AGAAGCTGGG AAATAGTCTT CTAAGGCGAG GAGCTTCAAA GAAGACCCCA 
GCAAAGGTCT CCCCCAATCC TCGCAGTGGA ACCCGCCGTT CTCCACGCAT TGCCACCACC 
ACAACTGGCA CTGCTACTGT TGCCACCACT CCTCGGGCCA AGGGCAAGGT GAAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097809.1
CDS	622..6801
Translation	MGALGGPKAELTSDGNRKKAENRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_030241949.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241949.1

ORF Insert Sequence:

ATGGGAGCAT TGGGTGGCCC CAAAGCCGAG CTGACTTCAG ATGGGAATAG AAAGAAGGCA 
GAAAATCGGA AACATCCTTC ATCTACACAA TGCCTGGTAT CTGTGCAGAA AGTGATAGAG 
GGATCCGAGA TGGAACTGGC CAAGATGATA ATGCTGTTTT TATATCAGTC CACCATGAGC 
TCCAGAAACC TCCGGGACTG GGAGCAGTTT GAATATGGGG TTCAGGCTGA GTTAGCGGTC 
ATCCTGAAGT TTATGCTGGA TCATGAGGAG TCGCTAAACC TTACTGAGGA CTTAGAGAGT 
TTCTTGGAGA AAGTTCCTTA CACCCATGCC AGCACCCTCT CAGAAGAGCT TTCTCCACCC 
AGCCACCAGA CCAAGAGGAA GATTCGCTTC CTAGAAATAC AGAGGATTGC TTCATCTTCC 
AGTGAGAACA ACTTCCTCTC AGGCTCTCCA TCCTCCCCTA TGGGCGACAT ACTGCAGACC 
CCACAGTTCC AGATGAGACG ACTGAAGAAG CAGCTGGCAG ACGAGAGGAG CAACAGAGAT 
GACCTGGAGC TGGAGCTGTC TGAGAGCCTC AAGCTCCTCA CTGAGAAGGA CGCGCAGATC 
GCCATGATGC AGCAGCGCAT TGATCACCTG GCTCTGCTGA ATGAGAAGCA GGCAGCCAGC 
TCCCAGGAGC CCAGTGAGCT TGAGGAGCTC CGAGGCAAGA ATGAAAGCCT CACTGTGCGG 
CTGCATGAGA CTTTGAAGCA GTGTCAGAAC CTGAAGACCG AGAAGAGCCA GATGGATCGA 
AAGATTAGCC AGCTTTCTGA GGAGAATGGG GACCTTTCCT TTAAGGTTCG AGAGTTTGCA 
AACCACTTGC AACAACTTCA GGGTGCATTC AACGACCTGA TAGAGGAGCA CAGCAAGGCT 
TCTCAGGAGT GGGCAGAGAA GCAGGCTCGT CTGGAAAATG AGCTCAGCAC AGCCCTCCAG 
GATAAGAAAT GTCTTGAAGA GAAGAATGAA ATTCTTCAGG GAAAGCTTTC CCAGCTGGAA 
GATCAAGCAA CTCGGCTGCA GGAGAGCCCA GCCCCGGAGA AGGGTGAGGT GCTAGGAGAC 
GCCTTGCAGC TGGACACTCT AAAGCAAGAG GCAGCCAAAC TTGCCACAGA CAACACCCAG 
CTCCAAACCC GCGTAGAGAC ACTGGAGTGT GAACGTGGCA AGCAGGAAGC CCAGCTGCTT 
GCTGAACGGA GCCGCTTTGA AGACGAAAAG CAGCAGCTGG CCAGCTTGAT TGCTGACCTT 
CAGAGCTCCG TCTCTAACCT CAGCCAGGCC AAAGAGGAGT TAGAGCAAGC CTCCCAGGCC 
CAAGGGGCTC AGCTCACTGC CCAGTTGACC TCTATGACAG GGCTCAATGC CACCCTGCAG 
CAGCGAGATC AAGAGCTGGC CAGTCTGAAA GAACAGGCCA AAAAGGAACA GGCTCAGATG 
CTACAGACCA TGCAAGAGCA AGAACAGGCT GCCCAGGGCC TCCGCCAGCA GGTGGAGCAG 
CTGAGCAGCA GCCTGAAGCT GAAGGAGCAG CAATTAGAAG AGGCAGCCAA GGAGCAGGAG 
GCAACCAGAC AGGATCACGC CCAGCAACTG GCCATCGTTG CTGAGGCTCG AGAGGCCTCT 
CTAAGGGAAC GGGATACTGC TCGCCAGCAG CTAGAAACAG TGGAGAAGGA GAAGGATGCC 
AAGCTAGAGA GTCTGCAGCA GCAACTTCAG GCTGCCAATG ATGCTAGGGA CAATGCGCAG 
ACCTCGGTCA CACAGGCTCA GCAGGAAAAG GCAGAGCTGA GCCAAAAGAT AGGAGAGCTT 
CATGCCTGCA TTGAAGCTTC CCACCAGGAA CAGCGTCAGG TTCAAGCTCG TGTGACAGAG 
CTAGAAGCCC AGCTGAAAGC TGAGCAGCAA AAAACAACTG AGCGAGAAAA GGTGGTTCAG 
GAGAAGGCCC AGCTCCAGGA GCAGCTCCGG GCCCTTGAGG AGTCTTTGAA GATTACCAAA 
GGCAGCCTTG AAGAAGAGAA ACGCAGGGCT GCAGATGCCC TGAAGGAGCA GCAGTGCCGT 
GCCACTGAGA TGGAGGCAGA GTCTCGAAGC CTGATGGAGC AGCGGGAGCG GGAACAGAAG 
GAGTTAGAGC AAGAGAAGGC TGGGCGAAAG GGGCTGGAGG CCCGGATACA GCAGCTTGAA 
GAGGCTCATC AGGCTGAGAC TGAAGCCTTG CGGCATGAGC TGGCAGAGGC CACAGCTTCC 
CAGCACAGAG CTGAGAGTGA GTGTGAGCGG CTCATCAGAG AGGTTGAGAG CCGGCAGAAG 
CGGTTTGAAG CTAGACAGCA AGAGGAGGCA AGGTACGGTG CCATGTTCCA GGAACAGCTG 
ATGGCTTTGA AAGGGGAGAA GACTGGCCAA GAGGTCCAGG AGGAGGCAGT AGAGATCCAC 
AGTGAAGGCC AGCCAGGCCA GCAGCAGAGT CAGCTAGCCC AGCTTCATGC CAGTCTGGCC 
AAAGCCATCC AGCAGGTCCA GGAGAAGGAA GTGAGAGCAC AGAAGCTGGT AGATGATCTC 
TCAGCTCTGC AGGAGAAGAT GGCTGCCACT AACAAGGAGG TGGCCTGTCT GAAGACCTTG 
GTGCTCAAGG CAGGTGAGCA GCAGGAAACA GCCTCACTTG AGCTGCTCAA AGAGCCCCCG 
AGAGCTGCAA ACAGAGCGTC TGACCAGCTA GGAGAACAGC AGGGACGGCC ATTCTCTAGT 
ACACACGCAG CAGTAAAAGC TATGGAGCGT GAAGCTGAGC AGATGGGCGG TGAACTGGAG 
AGGCTGCGGG CAGCACTGAT CAAGAGCCAG GGCCAGCAGC AAGAGGAGCG TGGGCAACAG 
GAGAGGGAGG TAGCACGGCT GACCCAGGAG CGGGGCCAGG CCCAGGCTGA CCTGGCTCAG 
GAGAAGGCAG CCAAGGCAGA GCTGGAGATG CGACTGCAGA ACACCCTCAA TGAGCAGCGT 
GTGGAGTTTG CTGCCCTACA GGAAGCACTG GCCCATGCCC TGACAGAAAA GGAAGGGACA 
GACCAAGAGC TTGCCAAACT TCGAGGGCAG GAGGCAGCTC AAAGAACAGA GCTGAAGGAG 
CTTCAACAGA CTTTGGAACA GCTGAAAATA CAGTTAGTGA AGAAAGAAAA AGAGCACCCT 
GCAGGGGGTG CCAGTGGAGA AGATGCTTCT GGCCCAGGAA CCCAGTCGGA GACAGCTGGA 
AAGACAGATG CACCAGGCCC TGAGTTGCAG GCACTTCGGG CAGAGATCAG TAAGCTAGAG 
CAGCAGTGTC AGCAGCAGCA GCAACAGGTG GAAGGCCTGA CACACAGCCT CAAGTCTGAG 
CGGGCTTGCC GGGCCGAGCA AGACAAAGCC CTGGAGACAC TGCAGGGCCA GTTAGAAGAG 
AAGGCTCGAG AGCTAGGGCA TAATCAGGCT GCCTCAGCTT CAGCTCAGAG AGAGTTGCAA 
GCCCTCCGTG CTAAAGCCCA GGACCATAGC AAAGCTGAGG AAGAGTGGAA GGCCCAGGTA 
GCCCGGGGCC AGCAGGAGGC TGAAAGAAAA AGCAGTCTTA TCAGCAGCTT GGAAGAGGAG 
GTATCCATCC TGAATCGCCA AGTCCTAGAG AAGGAAGGGG AGAGCAAGGA GTTGAAGCGC 
CTGGTTGTAG CTGAATCCGA GAAGAGCCAG AAGCTGGAAG AGAGGCTGCG CCTGCTTCAA 
GTAGAAACAG CCAGCAATAG TGCCAGGGCA GCAGAACGCA GCTCAGCTCT ACGAGAGGAG 
GTCCAGAGCC TCCGGGAAGA GGTGGAGAAA CAGCGTGTAG TTTCAGAGAA CTCGCGGCAG 
GAGTTGGCCT CACAAGCAGA GCGAGCTGAG GAGTTGGGCC AAGAATTGAA GGCCTGGCAG 
GAGAAGTTTT TCCAGAAGGA GCAAGCTCTC TCTGCCCTGC AGCTGGAGCA CACCAGCACA 
CAGGCCCTGG TAAGTGAGCT GCTGCCTGCC AAGCACCTTT GTCAGCAGCT GCAAGCTGAG 
CAAGCGGCTG CCGAGAAGCG CTTCCGGGAG GAGCTCGAGC AGAGCAAGCA GGCAGCTGGT 
GGGCTCCAGG CAGAGCTGAT GCGGGCCCAG CGGGAGCTCG GAGAGCTCGG ATCTTTGCGG 
CAGAAGATAG TTGAGCAGGA GCGAGCAGCC CAGCAGCTGC GGGCAGAGAA GGCCAGCTAT 
GCAGAGCAGC TGAGCATGCT GAAGAAGGCC CATGGCCTGC TGGCAGAGGA GAACCGGGGG 
CTGGGAGAGC GGGCCAACCT TGGCCGGCAG TTTCTGGAAG TAGAACTAGA TCAAGCCCGG 
GAGAAATATG TCCAGGAGTT GGCTGCTGTG CGTACTGATG CTGAGACCCA CTTGGCTGAA 
ATGCGACAAG AAGCACAGAG TACTAGCCGG GAGCTGGAGG TGATGACTGC CAAGTACGAA 
GGTGCCAAGG TGAAGGTCCT AGAGGAAAGG CAGAGGTTCC AAGAAGAGAG GCAGAAACTC 
ACTGCCCAGG TGGAGCAACT AGAGGTATTT CAAAGAGAGC AGACTAAGCA GGTGGAAGAA 
CTAAGTAAGA AGCTGACAGA GCATGACCAA GCCAGCAAGG TGCAGCAACA GAAGCTAAAG 
GCTTTCCAGG CCCAGAGAGG TGAAAGCCAG CAAGAGGTCC AGCGCCTCCA GACCCAGCTG 
AACGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCAG CTGAGCATTA CAAGCTCCAG 
ATGGAGAAAG CCAAGACCCA TTATGATGCC AAGAAGCAGC AGAACCAAAA GCTGCAGGAA 
CAGCTTCAAG ACTTGGAGGA ACTGCAGAAG GAGAACAAGG AGCTGCGGTC TGAAGCTGAA 
CGTCTGGGCC GTGAGCTGCA GCAGGCAGGG CTGAAGACCA AGGAAGCTGA ACAGACCTGC 
CGTCACCTTA CAGCCCAGGT GCGCAGCCTG GAGGCCCAGG TTGCCCATGC AGACCAGCAG 
CTTCGTGACT TGGGCAAATT CCAGGTGGCA ACTGATGCCT TAAAGAGCCG TGAGCCTCAA 
GTGAAACCCC AGCTGGACTT AAGTATTGAC AGCCTGGATC TGAGTTTGGA GGAGGGAACC 
CCATGCAGTG TGGCTAGCAA GCTGCCTCGT ACCCAACCAG ATGGTACCAG TGTCCCTGGA 
GAGCCAGCTT CACCTATCTC CCAGCGCCTG CCCCCCAAAG TGGAATCCCT GGAGAGCCTC 
TACTTCACCC CCACTCCTGC TCGGGGTCAG GCCCCCTTGG AGACTAGCCT TGACTCTCTG 
GGGGATGCCT TCCCTGACTC CGGCCGTAAG ACCCGCTCTG CTCGCCGCCG TACCACACAA 
ATCATCAACA TCACTATGAC CAAGAAATTA GAATTGGAAG AGCCAGACAG CGCCAACTCA 
TCCTTCTACA GCACGCAGTC TGCTCCTGCT TCCCAGGCCA ACTTGCGAGC CACTTCCTCC 
ACTCAGTCTC TGGCCCGCTT GGGTTCTCCT GATGATGGCA ACTCCGCTCT GCTTAGCCTG 
CCTGGCTACC GGCCTACCAC ACGAAGCTCT GCTCGTCGCT CCCAGGCCAG GATGTCCAGT 
GGGGCCCCCC AAGGGAGGAA TAGCTTCTAC ATGGGCACTT GCCAGGATGA GCCCGAGCAG 
CTGGATGATT GGAACCGCAT CGCAGAGTTG CAGCAGCGCA ACCGAGTATG CCCCCCGCAC 
CTGAAGACCT GCTACCCTCT GGAGTCCAGG CCTACCCTGA GCCTGGCCAC CATCACTGAT 
GAGGAGATGA AGACTGGAGA CCCCAGGGAA ACCTTGCGTA GAGCCAGCAT GCAGCCAGCT 
CAGATTGCTG AGGGTGTTGG CATAACTACC AGGCAGCAGC GCAAACGAGT TTCCTCTGAG 
ACCCACCAGG GCCCTGGCAC TCCTGAGTCC AAGAAAGCTA CCAGCTGCTT TCCACGCCCC 
ATGACCCCCA GGGACCGGCA TGAAGGACGT AAGCAGAGCA GCACTGCTGA CACCCAGAAG 
AAAGCAGCTC CAGTTCTCAA ACAGGCGGAC CGGCGTCAGT CAATGGCTTT CAGCATCCTT 
AACACACCTA AGAAGCTGGG AAATAGTCTT CTAAGGCGAG GAGCTTCAAA GAAGACCCCA 
GCAAAGGTCT CCCCCAATCC TCGCAGTGGA ACCCGCCGTT CTCCACGCAT TGCCACCACC 
ACAACTGGCA CTGCTACTGT TGCCACCACT CCTCGGGCCA AGGGCAAGGT GAAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_030241950.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097810.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X11, mRNA.

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CloneID	OMu89308
Clone ID Related Accession (Same CDS sequence)	XM_030241950.1
Accession Version	XM_030241950.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCAT TGGGTGGCCC CAAAGCCGAG CTGACTTCAG ATGGGAATAG AAAGAAGGCA 
GAAAATCGGA AACATCCTTC ATCTACACAA TGCCTGGTAT CTGTGCAGAA AGTGATAGAG 
GGATCCGAGA TGGAACTGGC CAAGATGATA ATGCTGTTTT TATATCAGTC CACCATGAGC 
TCCAGAAACC TCCGGGACTG GGAGCAGTTT GAATATGGGG TTCAGGCTGA GTTAGCGGTC 
ATCCTGAAGT TTATGCTGGA TCATGAGGAG TCGCTAAACC TTACTGAGGA CTTAGAGAGT 
TTCTTGGAGA AAGTTCCTTA CACCCATGCC AGCACCCTCT CAGAAGAGCT TTCTCCACCC 
AGCCACCAGA CCAAGAGGAA GATTCGCTTC CTAGAAATAC AGAGGATTGC TTCATCTTCC 
AGTGAGAACA ACTTCCTCTC AGGCTCTCCA TCCTCCCCTA TGGGCGACAT ACTGCAGACC 
CCACAGTTCC AGATGAGACG ACTGAAGAAG CAGCTGGCAG ACGAGAGGAG CAACAGAGAT 
GACCTGGAGC TGGAGCTGTC TGAGAGCCTC AAGCTCCTCA CTGAGAAGGA CGCGCAGATC 
GCCATGATGC AGCAGCGCAT TGATCACCTG GCTCTGCTGA ATGAGAAGCA GGCAGCCAGC 
TCCCAGGAGC CCAGTGAGCT TGAGGAGCTC CGAGGCAAGA ATGAAAGCCT CACTGTGCGG 
CTGCATGAGA CTTTGAAGCA GTGTCAGAAC CTGAAGACCG AGAAGAGCCA GATGGATCGA 
AAGATTAGCC AGCTTTCTGA GGAGAATGGG GACCTTTCCT TTAAGGTTCG AGAGTTTGCA 
AACCACTTGC AACAACTTCA GGGTGCATTC AACGACCTGA TAGAGGAGCA CAGCAAGGCT 
TCTCAGGAGT GGGCAGAGAA GCAGGCTCGT CTGGAAAATG AGCTCAGCAC AGCCCTCCAG 
GATAAGAAAT GTCTTGAAGA GAAGAATGAA ATTCTTCAGG GAAAGCTTTC CCAGCTGGAA 
GATCAAGCAA CTCGGCTGCA GGAGAGCCCA GCCCCGGAGA AGGGTGAGGT GCTAGGAGAC 
GCCTTGCAGC TGGACACTCT AAAGCAAGAG GCAGCCAAAC TTGCCACAGA CAACACCCAG 
CTCCAAACCC GCGTAGAGAC ACTGGAGTGT GAACGTGGCA AGCAGGAAGC CCAGCTGCTT 
GCTGAACGGA GCCGCTTTGA AGACGAAAAG CAGCAGCTGG CCAGCTTGAT TGCTGACCTT 
CAGAGCTCCG TCTCTAACCT CAGCCAGGCC AAAGAGGAGT TAGAGCAAGC CTCCCAGGCC 
CAAGGGGCTC AGCTCACTGC CCAGTTGACC TCTATGACAG GGCTCAATGC CACCCTGCAG 
CAGCGAGATC AAGAGCTGGC CAGTCTGAAA GAACAGGCCA AAAAGGAACA GGCTCAGATG 
CTACAGACCA TGCAAGAGCA AGAACAGGCT GCCCAGGGCC TCCGCCAGCA GGTGGAGCAG 
CTGAGCAGCA GCCTGAAGCT GAAGGAGCAG CAATTAGAAG AGGCAGCCAA GGAGCAGGAG 
GCAACCAGAC AGGATCACGC CCAGCAACTG GCCATCGTTG CTGAGGCTCG AGAGGCCTCT 
CTAAGGGAAC GGGATACTGC TCGCCAGCAG CTAGAAACAG TGGAGAAGGA GAAGGATGCC 
AAGCTAGAGA GTCTGCAGCA GCAACTTCAG GCTGCCAATG ATGCTAGGGA CAATGCGCAG 
ACCTCGGTCA CACAGGCTCA GCAGGAAAAG GCAGAGCTGA GCCAAAAGAT AGGAGAGCTT 
CATGCCTGCA TTGAAGCTTC CCACCAGGAA CAGCGTCAGG TTCAAGCTCG TGTGACAGAG 
CTAGAAGCCC AGCTGAAAGC TGAGCAGCAA AAAACAACTG AGCGAGAAAA GGTGGTTCAG 
GAGAAGGCCC AGCTCCAGGA GCAGCTCCGG GCCCTTGAGG AGTCTTTGAA GATTACCAAA 
GGCAGCCTTG AAGAAGAGAA ACGCAGGGCT GCAGATGCCC TGAAGGAGCA GCAGTGCCGT 
GCCACTGAGA TGGAGGCAGA GTCTCGAAGC CTGATGGAGC AGCGGGAGCG GGAACAGAAG 
GAGTTAGAGC AAGAGAAGGC TGGGCGAAAG GGGCTGGAGG CCCGGATACA GCAGCTTGAA 
GAGGCTCATC AGGCTGAGAC TGAAGCCTTG CGGCATGAGC TGGCAGAGGC CACAGCTTCC 
CAGCACAGAG CTGAGAGTGA GTGTGAGCGG CTCATCAGAG AGGTTGAGAG CCGGCAGAAG 
CGGTTTGAAG CTAGACAGCA AGAGGAGGCA AGGTACGGTG CCATGTTCCA GGAACAGCTG 
ATGGCTTTGA AAGGGGAGAA GACTGGCCAA GAGGTCCAGG AGGAGGCAGT AGAGATCCAC 
AGTGAAGGCC AGCCAGGCCA GCAGCAGAGT CAGCTAGCCC AGCTTCATGC CAGTCTGGCC 
AAAGCCATCC AGCAGGTCCA GGAGAAGGAA GTGAGAGCAC AGAAGCTGGT AGATGATCTC 
TCAGCTCTGC AGGAGAAGAT GGCTGCCACT AACAAGGAGG TGGCCTGTCT GAAGACCTTG 
GTGCTCAAGG CAGGTGAGCA GCAGGAAACA GCCTCACTTG AGCTGCTCAA AGAGCCCCCG 
AGAGCTGCAA ACAGAGCGTC TGACCAGCTA GGAGAACAGC AGGGACGGCC ATTCTCTAGT 
ACACACGCAG CAGTAAAAGC TATGGAGCGT GAAGCTGAGC AGATGGGCGG TGAACTGGAG 
AGGCTGCGGG CAGCACTGAT CAAGAGCCAG GGCCAGCAGC AAGAGGAGCG TGGGCAACAG 
GAGAGGGAGG TAGCACGGCT GACCCAGGAG CGGGGCCAGG CCCAGGCTGA CCTGGCTCAG 
GAGAAGGCAG CCAAGGCAGA GCTGGAGATG CGACTGCAGA ACACCCTCAA TGAGCAGCGT 
GTGGAGTTTG CTGCCCTACA GGAAGCACTG GCCCATGCCC TGACAGAAAA GGAAGGGACA 
GACCAAGAGC TTGCCAAACT TCGAGGGCAG GAGGCAGCTC AAAGAACAGA GCTGAAGGAG 
CTTCAACAGA CTTTGGAACA GCTGAAAATA CAGTTAGTGA AGAAAGAAAA AGAGCACCCT 
GCAGGGGGTG CCAGTGGAGA AGATGCTTCT GGCCCAGGAA CCCAGTCGGA GACAGCTGGA 
AAGACAGATG CACCAGGCCC TGAGTTGCAG GCACTTCGGG CAGAGATCAG TAAGCTAGAG 
CAGCAGTGTC AGCAGCAGCA GCAACAGGTG GAAGGCCTGA CACACAGCCT CAAGTCTGAG 
CGGGCTTGCC GGGCCGAGCA AGACAAAGCC CTGGAGACAC TGCAGGGCCA GTTAGAAGAG 
AAGGCTCGAG AGCTAGGGCA TAATCAGGCT GCCTCAGCTT CAGCTCAGAG AGAGTTGCAA 
GCCCTCCGTG CTAAAGCCCA GGACCATAGC AAAGCTGAGG AAGAGTGGAA GGCCCAGGTA 
GCCCGGGGCC AGCAGGAGGC TGAAAGAAAA AGCAGTCTTA TCAGCAGCTT GGAAGAGGAG 
GTATCCATCC TGAATCGCCA AGTCCTAGAG AAGGAAGGGG AGAGCAAGGA GTTGAAGCGC 
CTGGTTGTAG CTGAATCCGA GAAGAGCCAG AAGCTGGAAG AGAGGCTGCG CCTGCTTCAA 
GTAGAAACAG CCAGCAATAG TGCCAGGGCA GCAGAACGCA GCTCAGCTCT ACGAGAGGAG 
GTCCAGAGCC TCCGGGAAGA GGTGGAGAAA CAGCGTGTAG TTTCAGAGAA CTCGCGGCAG 
GAGTTGGCCT CACAAGCAGA GCGAGCTGAG GAGTTGGGCC AAGAATTGAA GGCCTGGCAG 
GAGAAGTTTT TCCAGAAGGA GCAAGCTCTC TCTGCCCTGC AGCTGGAGCA CACCAGCACA 
CAGGCCCTGG TAAGTGAGCT GCTGCCTGCC AAGCACCTTT GTCAGCAGCT GCAAGCTGAG 
CAAGCGGCTG CCGAGAAGCG CTTCCGGGAG GAGCTCGAGC AGAGCAAGCA GGCAGCTGGT 
GGGCTCCAGG CAGAGCTGAT GCGGGCCCAG CGGGAGCTCG GAGAGCTCGG ATCTTTGCGG 
CAGAAGATAG TTGAGCAGGA GCGAGCAGCC CAGCAGCTGC GGGCAGAGAA GGCCAGCTAT 
GCAGAGCAGC TGAGCATGCT GAAGAAGGCC CATGGCCTGC TGGCAGAGGA GAACCGGGGG 
CTGGGAGAGC GGGCCAACCT TGGCCGGCAG TTTCTGGAAG TAGAACTAGA TCAAGCCCGG 
GAGAAATATG TCCAGGAGTT GGCTGCTGTG CGTACTGATG CTGAGACCCA CTTGGCTGAA 
ATGCGACAAG AAGCACAGAG TACTAGCCGG GAGCTGGAGG TGATGACTGC CAAGTACGAA 
GGTGCCAAGG TGAAGGTCCT AGAGGAAAGG CAGAGGTTCC AAGAAGAGAG GCAGAAACTC 
ACTGCCCAGG TGGAAGAACT AAGTAAGAAG CTGACAGAGC ATGACCAAGC CAGCAAGGTG 
CAGCAACAGA AGCTAAAGGC TTTCCAGGCC CAGAGAGGTG AAAGCCAGCA AGAGGTCCAG 
CGCCTCCAGA CCCAGCTGAA CGAGCTGCAG GCCCAGCTGA GCCAGAAGGA GCAGGCAGCT 
GAGCATTACA AGCTCCAGAT GGAGAAAGCC AAGACCCATT ATGATGCCAA GAAGCAGCAG 
AACCAAAAGC TGCAGGAACA GCTTCAAGAC TTGGAGGAAC TGCAGAAGGA GAACAAGGAG 
CTGCGGTCTG AAGCTGAACG TCTGGGCCGT GAGCTGCAGC AGGCAGGGCT GAAGACCAAG 
GAAGCTGAAC AGACCTGCCG TCACCTTACA GCCCAGGTGC GCAGCCTGGA GGCCCAGGTT 
GCCCATGCAG ACCAGCAGCT TCGTGACTTG GGCAAATTCC AGGTGGCAAC TGATGCCTTA 
AAGAGCCGTG AGCCTCAAGT GAAACCCCAG CTGGACTTAA GTATTGACAG CCTGGATCTG 
AGTTTGGAGG AGGGAACCCC ATGCAGTGTG GCTAGCAAGC TGCCTCGTAC CCAACCAGAT 
GGTACCAGTG TCCCTGGAGA GCCAGCTTCA CCTATCTCCC AGCGCCTGCC CCCCAAAGTG 
GAATCCCTGG AGAGCCTCTA CTTCACCCCC ACTCCTGCTC GGGGTCAGGC CCCCTTGGAG 
ACTAGCCTTG ACTCTCTGGG GGATGCCTTC CCTGACTCCG GCCGTAAGAC CCGCTCTGCT 
CGCCGCCGTA CCACACAAAT CATCAACATC ACTATGACCA AGAAATTAGA ATTGGAAGAG 
CCAGACAGCG CCAACTCATC CTTCTACAGC ACGCAGTCTG CTCCTGCTTC CCAGGCCAAC 
TTGCGAGCCA CTTCCTCCAC TCAGTCTCTG GCCCGCTTGG GTTCTCCTGA TGATGGCAAC 
TCCGCTCTGC TTAGCCTGCC TGGCTACCGG CCTACCACAC GAAGCTCTGC TCGTCGCTCC 
CAGGCCAGGA TGTCCAGTGG GGCCCCCCAA GGGAGGAATA GCTTCTACAT GGGCACTTGC 
CAGGATGAGC CCGAGCAGCT GGATGATTGG AACCGCATCG CAGAGTTGCA GCAGCGCAAC 
CGAGTATGCC CCCCGCACCT GAAGACCTGC TACCCTCTGG AGTCCAGGCC TACCCTGAGC 
CTGGCCACCA TCACTGATGA GGAGATGAAG ACTGGAGACC CCAGGGAAAC CTTGCGTAGA 
GCCAGCATGC AGCCAGCTCA GATTGCTGAG GGTGTTGGCA TAACTACCAG GCAGCAGCGC 
AAACGAGTTT CCTCTGAGAC CCACCAGGGC CCTGGCACTC CTGAGTCCAA GAAAGCTACC 
AGCTGCTTTC CACGCCCCAT GACCCCCAGG GACCGGCATG AAGGACGTAA GCAGAGCAGC 
ACTGCTGACA CCCAGAAGAA AGCAGCTCCA GTTCTCAAAC AGGCGGACCG GCGTCAGTCA 
ATGGCTTTCA GCATCCTTAA CACACCTAAG AAGCTGGGAA ATAGTCTTCT AAGGCGAGGA 
GCTTCAAAGA AGACCCCAGC AAAGGTCTCC CCCAATCCTC GCAGTGGAAC CCGCCGTTCT 
CCACGCATTG CCACCACCAC AACTGGCACT GCTACTGTTG CCACCACTCC TCGGGCCAAG 
GGCAAGGTGA AGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097810.1
CDS	621..6758
Translation	MGALGGPKAELTSDGNRKKAENRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_030241950.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241950.1

ORF Insert Sequence:

ATGGGAGCAT TGGGTGGCCC CAAAGCCGAG CTGACTTCAG ATGGGAATAG AAAGAAGGCA 
GAAAATCGGA AACATCCTTC ATCTACACAA TGCCTGGTAT CTGTGCAGAA AGTGATAGAG 
GGATCCGAGA TGGAACTGGC CAAGATGATA ATGCTGTTTT TATATCAGTC CACCATGAGC 
TCCAGAAACC TCCGGGACTG GGAGCAGTTT GAATATGGGG TTCAGGCTGA GTTAGCGGTC 
ATCCTGAAGT TTATGCTGGA TCATGAGGAG TCGCTAAACC TTACTGAGGA CTTAGAGAGT 
TTCTTGGAGA AAGTTCCTTA CACCCATGCC AGCACCCTCT CAGAAGAGCT TTCTCCACCC 
AGCCACCAGA CCAAGAGGAA GATTCGCTTC CTAGAAATAC AGAGGATTGC TTCATCTTCC 
AGTGAGAACA ACTTCCTCTC AGGCTCTCCA TCCTCCCCTA TGGGCGACAT ACTGCAGACC 
CCACAGTTCC AGATGAGACG ACTGAAGAAG CAGCTGGCAG ACGAGAGGAG CAACAGAGAT 
GACCTGGAGC TGGAGCTGTC TGAGAGCCTC AAGCTCCTCA CTGAGAAGGA CGCGCAGATC 
GCCATGATGC AGCAGCGCAT TGATCACCTG GCTCTGCTGA ATGAGAAGCA GGCAGCCAGC 
TCCCAGGAGC CCAGTGAGCT TGAGGAGCTC CGAGGCAAGA ATGAAAGCCT CACTGTGCGG 
CTGCATGAGA CTTTGAAGCA GTGTCAGAAC CTGAAGACCG AGAAGAGCCA GATGGATCGA 
AAGATTAGCC AGCTTTCTGA GGAGAATGGG GACCTTTCCT TTAAGGTTCG AGAGTTTGCA 
AACCACTTGC AACAACTTCA GGGTGCATTC AACGACCTGA TAGAGGAGCA CAGCAAGGCT 
TCTCAGGAGT GGGCAGAGAA GCAGGCTCGT CTGGAAAATG AGCTCAGCAC AGCCCTCCAG 
GATAAGAAAT GTCTTGAAGA GAAGAATGAA ATTCTTCAGG GAAAGCTTTC CCAGCTGGAA 
GATCAAGCAA CTCGGCTGCA GGAGAGCCCA GCCCCGGAGA AGGGTGAGGT GCTAGGAGAC 
GCCTTGCAGC TGGACACTCT AAAGCAAGAG GCAGCCAAAC TTGCCACAGA CAACACCCAG 
CTCCAAACCC GCGTAGAGAC ACTGGAGTGT GAACGTGGCA AGCAGGAAGC CCAGCTGCTT 
GCTGAACGGA GCCGCTTTGA AGACGAAAAG CAGCAGCTGG CCAGCTTGAT TGCTGACCTT 
CAGAGCTCCG TCTCTAACCT CAGCCAGGCC AAAGAGGAGT TAGAGCAAGC CTCCCAGGCC 
CAAGGGGCTC AGCTCACTGC CCAGTTGACC TCTATGACAG GGCTCAATGC CACCCTGCAG 
CAGCGAGATC AAGAGCTGGC CAGTCTGAAA GAACAGGCCA AAAAGGAACA GGCTCAGATG 
CTACAGACCA TGCAAGAGCA AGAACAGGCT GCCCAGGGCC TCCGCCAGCA GGTGGAGCAG 
CTGAGCAGCA GCCTGAAGCT GAAGGAGCAG CAATTAGAAG AGGCAGCCAA GGAGCAGGAG 
GCAACCAGAC AGGATCACGC CCAGCAACTG GCCATCGTTG CTGAGGCTCG AGAGGCCTCT 
CTAAGGGAAC GGGATACTGC TCGCCAGCAG CTAGAAACAG TGGAGAAGGA GAAGGATGCC 
AAGCTAGAGA GTCTGCAGCA GCAACTTCAG GCTGCCAATG ATGCTAGGGA CAATGCGCAG 
ACCTCGGTCA CACAGGCTCA GCAGGAAAAG GCAGAGCTGA GCCAAAAGAT AGGAGAGCTT 
CATGCCTGCA TTGAAGCTTC CCACCAGGAA CAGCGTCAGG TTCAAGCTCG TGTGACAGAG 
CTAGAAGCCC AGCTGAAAGC TGAGCAGCAA AAAACAACTG AGCGAGAAAA GGTGGTTCAG 
GAGAAGGCCC AGCTCCAGGA GCAGCTCCGG GCCCTTGAGG AGTCTTTGAA GATTACCAAA 
GGCAGCCTTG AAGAAGAGAA ACGCAGGGCT GCAGATGCCC TGAAGGAGCA GCAGTGCCGT 
GCCACTGAGA TGGAGGCAGA GTCTCGAAGC CTGATGGAGC AGCGGGAGCG GGAACAGAAG 
GAGTTAGAGC AAGAGAAGGC TGGGCGAAAG GGGCTGGAGG CCCGGATACA GCAGCTTGAA 
GAGGCTCATC AGGCTGAGAC TGAAGCCTTG CGGCATGAGC TGGCAGAGGC CACAGCTTCC 
CAGCACAGAG CTGAGAGTGA GTGTGAGCGG CTCATCAGAG AGGTTGAGAG CCGGCAGAAG 
CGGTTTGAAG CTAGACAGCA AGAGGAGGCA AGGTACGGTG CCATGTTCCA GGAACAGCTG 
ATGGCTTTGA AAGGGGAGAA GACTGGCCAA GAGGTCCAGG AGGAGGCAGT AGAGATCCAC 
AGTGAAGGCC AGCCAGGCCA GCAGCAGAGT CAGCTAGCCC AGCTTCATGC CAGTCTGGCC 
AAAGCCATCC AGCAGGTCCA GGAGAAGGAA GTGAGAGCAC AGAAGCTGGT AGATGATCTC 
TCAGCTCTGC AGGAGAAGAT GGCTGCCACT AACAAGGAGG TGGCCTGTCT GAAGACCTTG 
GTGCTCAAGG CAGGTGAGCA GCAGGAAACA GCCTCACTTG AGCTGCTCAA AGAGCCCCCG 
AGAGCTGCAA ACAGAGCGTC TGACCAGCTA GGAGAACAGC AGGGACGGCC ATTCTCTAGT 
ACACACGCAG CAGTAAAAGC TATGGAGCGT GAAGCTGAGC AGATGGGCGG TGAACTGGAG 
AGGCTGCGGG CAGCACTGAT CAAGAGCCAG GGCCAGCAGC AAGAGGAGCG TGGGCAACAG 
GAGAGGGAGG TAGCACGGCT GACCCAGGAG CGGGGCCAGG CCCAGGCTGA CCTGGCTCAG 
GAGAAGGCAG CCAAGGCAGA GCTGGAGATG CGACTGCAGA ACACCCTCAA TGAGCAGCGT 
GTGGAGTTTG CTGCCCTACA GGAAGCACTG GCCCATGCCC TGACAGAAAA GGAAGGGACA 
GACCAAGAGC TTGCCAAACT TCGAGGGCAG GAGGCAGCTC AAAGAACAGA GCTGAAGGAG 
CTTCAACAGA CTTTGGAACA GCTGAAAATA CAGTTAGTGA AGAAAGAAAA AGAGCACCCT 
GCAGGGGGTG CCAGTGGAGA AGATGCTTCT GGCCCAGGAA CCCAGTCGGA GACAGCTGGA 
AAGACAGATG CACCAGGCCC TGAGTTGCAG GCACTTCGGG CAGAGATCAG TAAGCTAGAG 
CAGCAGTGTC AGCAGCAGCA GCAACAGGTG GAAGGCCTGA CACACAGCCT CAAGTCTGAG 
CGGGCTTGCC GGGCCGAGCA AGACAAAGCC CTGGAGACAC TGCAGGGCCA GTTAGAAGAG 
AAGGCTCGAG AGCTAGGGCA TAATCAGGCT GCCTCAGCTT CAGCTCAGAG AGAGTTGCAA 
GCCCTCCGTG CTAAAGCCCA GGACCATAGC AAAGCTGAGG AAGAGTGGAA GGCCCAGGTA 
GCCCGGGGCC AGCAGGAGGC TGAAAGAAAA AGCAGTCTTA TCAGCAGCTT GGAAGAGGAG 
GTATCCATCC TGAATCGCCA AGTCCTAGAG AAGGAAGGGG AGAGCAAGGA GTTGAAGCGC 
CTGGTTGTAG CTGAATCCGA GAAGAGCCAG AAGCTGGAAG AGAGGCTGCG CCTGCTTCAA 
GTAGAAACAG CCAGCAATAG TGCCAGGGCA GCAGAACGCA GCTCAGCTCT ACGAGAGGAG 
GTCCAGAGCC TCCGGGAAGA GGTGGAGAAA CAGCGTGTAG TTTCAGAGAA CTCGCGGCAG 
GAGTTGGCCT CACAAGCAGA GCGAGCTGAG GAGTTGGGCC AAGAATTGAA GGCCTGGCAG 
GAGAAGTTTT TCCAGAAGGA GCAAGCTCTC TCTGCCCTGC AGCTGGAGCA CACCAGCACA 
CAGGCCCTGG TAAGTGAGCT GCTGCCTGCC AAGCACCTTT GTCAGCAGCT GCAAGCTGAG 
CAAGCGGCTG CCGAGAAGCG CTTCCGGGAG GAGCTCGAGC AGAGCAAGCA GGCAGCTGGT 
GGGCTCCAGG CAGAGCTGAT GCGGGCCCAG CGGGAGCTCG GAGAGCTCGG ATCTTTGCGG 
CAGAAGATAG TTGAGCAGGA GCGAGCAGCC CAGCAGCTGC GGGCAGAGAA GGCCAGCTAT 
GCAGAGCAGC TGAGCATGCT GAAGAAGGCC CATGGCCTGC TGGCAGAGGA GAACCGGGGG 
CTGGGAGAGC GGGCCAACCT TGGCCGGCAG TTTCTGGAAG TAGAACTAGA TCAAGCCCGG 
GAGAAATATG TCCAGGAGTT GGCTGCTGTG CGTACTGATG CTGAGACCCA CTTGGCTGAA 
ATGCGACAAG AAGCACAGAG TACTAGCCGG GAGCTGGAGG TGATGACTGC CAAGTACGAA 
GGTGCCAAGG TGAAGGTCCT AGAGGAAAGG CAGAGGTTCC AAGAAGAGAG GCAGAAACTC 
ACTGCCCAGG TGGAAGAACT AAGTAAGAAG CTGACAGAGC ATGACCAAGC CAGCAAGGTG 
CAGCAACAGA AGCTAAAGGC TTTCCAGGCC CAGAGAGGTG AAAGCCAGCA AGAGGTCCAG 
CGCCTCCAGA CCCAGCTGAA CGAGCTGCAG GCCCAGCTGA GCCAGAAGGA GCAGGCAGCT 
GAGCATTACA AGCTCCAGAT GGAGAAAGCC AAGACCCATT ATGATGCCAA GAAGCAGCAG 
AACCAAAAGC TGCAGGAACA GCTTCAAGAC TTGGAGGAAC TGCAGAAGGA GAACAAGGAG 
CTGCGGTCTG AAGCTGAACG TCTGGGCCGT GAGCTGCAGC AGGCAGGGCT GAAGACCAAG 
GAAGCTGAAC AGACCTGCCG TCACCTTACA GCCCAGGTGC GCAGCCTGGA GGCCCAGGTT 
GCCCATGCAG ACCAGCAGCT TCGTGACTTG GGCAAATTCC AGGTGGCAAC TGATGCCTTA 
AAGAGCCGTG AGCCTCAAGT GAAACCCCAG CTGGACTTAA GTATTGACAG CCTGGATCTG 
AGTTTGGAGG AGGGAACCCC ATGCAGTGTG GCTAGCAAGC TGCCTCGTAC CCAACCAGAT 
GGTACCAGTG TCCCTGGAGA GCCAGCTTCA CCTATCTCCC AGCGCCTGCC CCCCAAAGTG 
GAATCCCTGG AGAGCCTCTA CTTCACCCCC ACTCCTGCTC GGGGTCAGGC CCCCTTGGAG 
ACTAGCCTTG ACTCTCTGGG GGATGCCTTC CCTGACTCCG GCCGTAAGAC CCGCTCTGCT 
CGCCGCCGTA CCACACAAAT CATCAACATC ACTATGACCA AGAAATTAGA ATTGGAAGAG 
CCAGACAGCG CCAACTCATC CTTCTACAGC ACGCAGTCTG CTCCTGCTTC CCAGGCCAAC 
TTGCGAGCCA CTTCCTCCAC TCAGTCTCTG GCCCGCTTGG GTTCTCCTGA TGATGGCAAC 
TCCGCTCTGC TTAGCCTGCC TGGCTACCGG CCTACCACAC GAAGCTCTGC TCGTCGCTCC 
CAGGCCAGGA TGTCCAGTGG GGCCCCCCAA GGGAGGAATA GCTTCTACAT GGGCACTTGC 
CAGGATGAGC CCGAGCAGCT GGATGATTGG AACCGCATCG CAGAGTTGCA GCAGCGCAAC 
CGAGTATGCC CCCCGCACCT GAAGACCTGC TACCCTCTGG AGTCCAGGCC TACCCTGAGC 
CTGGCCACCA TCACTGATGA GGAGATGAAG ACTGGAGACC CCAGGGAAAC CTTGCGTAGA 
GCCAGCATGC AGCCAGCTCA GATTGCTGAG GGTGTTGGCA TAACTACCAG GCAGCAGCGC 
AAACGAGTTT CCTCTGAGAC CCACCAGGGC CCTGGCACTC CTGAGTCCAA GAAAGCTACC 
AGCTGCTTTC CACGCCCCAT GACCCCCAGG GACCGGCATG AAGGACGTAA GCAGAGCAGC 
ACTGCTGACA CCCAGAAGAA AGCAGCTCCA GTTCTCAAAC AGGCGGACCG GCGTCAGTCA 
ATGGCTTTCA GCATCCTTAA CACACCTAAG AAGCTGGGAA ATAGTCTTCT AAGGCGAGGA 
GCTTCAAAGA AGACCCCAGC AAAGGTCTCC CCCAATCCTC GCAGTGGAAC CCGCCGTTCT 
CCACGCATTG CCACCACCAC AACTGGCACT GCTACTGTTG CCACCACTCC TCGGGCCAAG 
GGCAAGGTGA AGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( NM_133947.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598708.3 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), mRNA.

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CloneID	OMu13199
Clone ID Related Accession (Same CDS sequence)	XM_006507177.2 , XM_006507177.3 , NM_133947.3
Accession Version	NM_133947.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC049791.1 and AK032693.1. On Jul 24, 2009 this sequence version replaced NM_133947.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC049791.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AAGAACTAAG TAAGAAGCTG 
ACAGAGCATG ACCAAGCCAG CAAGGTGCAG CAACAGAAGC TAAAGGCTTT CCAGGCCCAG 
AGAGGTGAAA GCCAGCAAGA GGTCCAGCGC CTCCAGACCC AGCTGAACGA GCTGCAGGCC 
CAGCTGAGCC AGAAGGAGCA GGCAGCTGAG CATTACAAGC TCCAGATGGA GAAAGCCAAG 
ACCCATTATG ATGCCAAGAA GCAGCAGAAC CAAAAGCTGC AGGAACAGCT TCAAGACTTG 
GAGGAACTGC AGAAGGAGAA CAAGGAGCTG CGGTCTGAAG CTGAACGTCT GGGCCGTGAG 
CTGCAGCAGG CAGGGCTGAA GACCAAGGAA GCTGAACAGA CCTGCCGTCA CCTTACAGCC 
CAGGTGCGCA GCCTGGAGGC CCAGGTTGCC CATGCAGACC AGCAGCTTCG TGACTTGGGC 
AAATTCCAGG TGGCAACTGA TGCCTTAAAG AGCCGTGAGC CTCAAGTGAA ACCCCAGCTG 
GACTTAAGTA TTGACAGCCT GGATCTGAGT TTGGAGGAGG GAACCCCATG CAGTGTGGCT 
AGCAAGCTGC CTCGTACCCA ACCAGATGGT ACCAGTGTCC CTGGAGAGCC AGCTTCACCT 
ATCTCCCAGC GCCTGCCCCC CAAAGTGGAA TCCCTGGAGA GCCTCTACTT CACCCCCACT 
CCTGCTCGGG GTCAGGCCCC CTTGGAGACT AGCCTTGACT CTCTGGGGGA TGCCTTCCCT 
GACTCCGGCC GTAAGACCCG CTCTGCTCGC CGCCGTACCA CACAAATCAT CAACATCACT 
ATGACCAAGA AATTAGAATT GGAAGAGCCA GACAGCGCCA ACTCATCCTT CTACAGCACG 
CAGTCTGCTC CTGCTTCCCA GGCCAACTTG CGAGCCACTT CCTCCACTCA GTCTCTGGCC 
CGCTTGGGTT CTCCTGATGA TGGCAACTCC GCTCTGCTTA GCCTGCCTGG CTACCGGCCT 
ACCACACGAA GCTCTGCTCG TCGCTCCCAG GCCAGGATGT CCAGTGGGGC CCCCCAAGGG 
AGGAATAGCT TCTACATGGG CACTTGCCAG GATGAGCCCG AGCAGCTGGA TGATTGGAAC 
CGCATCGCAG AGTTGCAGCA GCGCAACCGA GTATGCCCCC CGCACCTGAA GACCTGCTAC 
CCTCTGGAGT CCAGGCCTAC CCTGAGCCTG GCCACCATCA CTGATGAGGA GATGAAGACT 
GGAGACCCCA GGGAAACCTT GCGTAGAGCC AGCATGCAGC CAGCTCAGAT TGCTGAGGGT 
GTTGGCATAA CTACCAGGCA GCAGCGCAAA CGAGTTTCCT CTGAGACCCA CCAGGGCCCT 
GGCACTCCTG AGTCCAAGAA AGCTACCAGC TGCTTTCCAC GCCCCATGAC CCCCAGGGAC 
CGGCATGAAG GACGTAAGCA GAGCAGCACT GCTGACACCC AGAAGAAAGC AGCTCCAGTT 
CTCAAACAGG CGGACCGGCG TCAGTCAATG GCTTTCAGCA TCCTTAACAC ACCTAAGAAG 
CTGGGAAATA GTCTTCTAAG GCGAGGAGCT TCAAAGAAGA CCCCAGCAAA GGTCTCCCCC 
AATCCTCGCA GTGGAACCCG CCGTTCTCCA CGCATTGCCA CCACCACAAC TGGCACTGCT 
ACTGTTGCCA CCACTCCTCG GGCCAAGGGC AAGGTGAAGC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598708.3
CDS	157..6441
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	NM_133947.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133947.3

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AAGAACTAAG TAAGAAGCTG 
ACAGAGCATG ACCAAGCCAG CAAGGTGCAG CAACAGAAGC TAAAGGCTTT CCAGGCCCAG 
AGAGGTGAAA GCCAGCAAGA GGTCCAGCGC CTCCAGACCC AGCTGAACGA GCTGCAGGCC 
CAGCTGAGCC AGAAGGAGCA GGCAGCTGAG CATTACAAGC TCCAGATGGA GAAAGCCAAG 
ACCCATTATG ATGCCAAGAA GCAGCAGAAC CAAAAGCTGC AGGAACAGCT TCAAGACTTG 
GAGGAACTGC AGAAGGAGAA CAAGGAGCTG CGGTCTGAAG CTGAACGTCT GGGCCGTGAG 
CTGCAGCAGG CAGGGCTGAA GACCAAGGAA GCTGAACAGA CCTGCCGTCA CCTTACAGCC 
CAGGTGCGCA GCCTGGAGGC CCAGGTTGCC CATGCAGACC AGCAGCTTCG TGACTTGGGC 
AAATTCCAGG TGGCAACTGA TGCCTTAAAG AGCCGTGAGC CTCAAGTGAA ACCCCAGCTG 
GACTTAAGTA TTGACAGCCT GGATCTGAGT TTGGAGGAGG GAACCCCATG CAGTGTGGCT 
AGCAAGCTGC CTCGTACCCA ACCAGATGGT ACCAGTGTCC CTGGAGAGCC AGCTTCACCT 
ATCTCCCAGC GCCTGCCCCC CAAAGTGGAA TCCCTGGAGA GCCTCTACTT CACCCCCACT 
CCTGCTCGGG GTCAGGCCCC CTTGGAGACT AGCCTTGACT CTCTGGGGGA TGCCTTCCCT 
GACTCCGGCC GTAAGACCCG CTCTGCTCGC CGCCGTACCA CACAAATCAT CAACATCACT 
ATGACCAAGA AATTAGAATT GGAAGAGCCA GACAGCGCCA ACTCATCCTT CTACAGCACG 
CAGTCTGCTC CTGCTTCCCA GGCCAACTTG CGAGCCACTT CCTCCACTCA GTCTCTGGCC 
CGCTTGGGTT CTCCTGATGA TGGCAACTCC GCTCTGCTTA GCCTGCCTGG CTACCGGCCT 
ACCACACGAA GCTCTGCTCG TCGCTCCCAG GCCAGGATGT CCAGTGGGGC CCCCCAAGGG 
AGGAATAGCT TCTACATGGG CACTTGCCAG GATGAGCCCG AGCAGCTGGA TGATTGGAAC 
CGCATCGCAG AGTTGCAGCA GCGCAACCGA GTATGCCCCC CGCACCTGAA GACCTGCTAC 
CCTCTGGAGT CCAGGCCTAC CCTGAGCCTG GCCACCATCA CTGATGAGGA GATGAAGACT 
GGAGACCCCA GGGAAACCTT GCGTAGAGCC AGCATGCAGC CAGCTCAGAT TGCTGAGGGT 
GTTGGCATAA CTACCAGGCA GCAGCGCAAA CGAGTTTCCT CTGAGACCCA CCAGGGCCCT 
GGCACTCCTG AGTCCAAGAA AGCTACCAGC TGCTTTCCAC GCCCCATGAC CCCCAGGGAC 
CGGCATGAAG GACGTAAGCA GAGCAGCACT GCTGACACCC AGAAGAAAGC AGCTCCAGTT 
CTCAAACAGG CGGACCGGCG TCAGTCAATG GCTTTCAGCA TCCTTAACAC ACCTAAGAAG 
CTGGGAAATA GTCTTCTAAG GCGAGGAGCT TCAAAGAAGA CCCCAGCAAA GGTCTCCCCC 
AATCCTCGCA GTGGAACCCG CCGTTCTCCA CGCATTGCCA CCACCACAAC TGGCACTGCT 
ACTGTTGCCA CCACTCCTCG GGCCAAGGGC AAGGTGAAGC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_006507177.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507240.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X8, mRNA.

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CloneID	OMu13199
Clone ID Related Accession (Same CDS sequence)	XM_006507177.2 , XM_006507177.3 , NM_133947.3
Accession Version	XM_006507177.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AAGAACTAAG TAAGAAGCTG 
ACAGAGCATG ACCAAGCCAG CAAGGTGCAG CAACAGAAGC TAAAGGCTTT CCAGGCCCAG 
AGAGGTGAAA GCCAGCAAGA GGTCCAGCGC CTCCAGACCC AGCTGAACGA GCTGCAGGCC 
CAGCTGAGCC AGAAGGAGCA GGCAGCTGAG CATTACAAGC TCCAGATGGA GAAAGCCAAG 
ACCCATTATG ATGCCAAGAA GCAGCAGAAC CAAAAGCTGC AGGAACAGCT TCAAGACTTG 
GAGGAACTGC AGAAGGAGAA CAAGGAGCTG CGGTCTGAAG CTGAACGTCT GGGCCGTGAG 
CTGCAGCAGG CAGGGCTGAA GACCAAGGAA GCTGAACAGA CCTGCCGTCA CCTTACAGCC 
CAGGTGCGCA GCCTGGAGGC CCAGGTTGCC CATGCAGACC AGCAGCTTCG TGACTTGGGC 
AAATTCCAGG TGGCAACTGA TGCCTTAAAG AGCCGTGAGC CTCAAGTGAA ACCCCAGCTG 
GACTTAAGTA TTGACAGCCT GGATCTGAGT TTGGAGGAGG GAACCCCATG CAGTGTGGCT 
AGCAAGCTGC CTCGTACCCA ACCAGATGGT ACCAGTGTCC CTGGAGAGCC AGCTTCACCT 
ATCTCCCAGC GCCTGCCCCC CAAAGTGGAA TCCCTGGAGA GCCTCTACTT CACCCCCACT 
CCTGCTCGGG GTCAGGCCCC CTTGGAGACT AGCCTTGACT CTCTGGGGGA TGCCTTCCCT 
GACTCCGGCC GTAAGACCCG CTCTGCTCGC CGCCGTACCA CACAAATCAT CAACATCACT 
ATGACCAAGA AATTAGAATT GGAAGAGCCA GACAGCGCCA ACTCATCCTT CTACAGCACG 
CAGTCTGCTC CTGCTTCCCA GGCCAACTTG CGAGCCACTT CCTCCACTCA GTCTCTGGCC 
CGCTTGGGTT CTCCTGATGA TGGCAACTCC GCTCTGCTTA GCCTGCCTGG CTACCGGCCT 
ACCACACGAA GCTCTGCTCG TCGCTCCCAG GCCAGGATGT CCAGTGGGGC CCCCCAAGGG 
AGGAATAGCT TCTACATGGG CACTTGCCAG GATGAGCCCG AGCAGCTGGA TGATTGGAAC 
CGCATCGCAG AGTTGCAGCA GCGCAACCGA GTATGCCCCC CGCACCTGAA GACCTGCTAC 
CCTCTGGAGT CCAGGCCTAC CCTGAGCCTG GCCACCATCA CTGATGAGGA GATGAAGACT 
GGAGACCCCA GGGAAACCTT GCGTAGAGCC AGCATGCAGC CAGCTCAGAT TGCTGAGGGT 
GTTGGCATAA CTACCAGGCA GCAGCGCAAA CGAGTTTCCT CTGAGACCCA CCAGGGCCCT 
GGCACTCCTG AGTCCAAGAA AGCTACCAGC TGCTTTCCAC GCCCCATGAC CCCCAGGGAC 
CGGCATGAAG GACGTAAGCA GAGCAGCACT GCTGACACCC AGAAGAAAGC AGCTCCAGTT 
CTCAAACAGG CGGACCGGCG TCAGTCAATG GCTTTCAGCA TCCTTAACAC ACCTAAGAAG 
CTGGGAAATA GTCTTCTAAG GCGAGGAGCT TCAAAGAAGA CCCCAGCAAA GGTCTCCCCC 
AATCCTCGCA GTGGAACCCG CCGTTCTCCA CGCATTGCCA CCACCACAAC TGGCACTGCT 
ACTGTTGCCA CCACTCCTCG GGCCAAGGGC AAGGTGAAGC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507240.1
CDS	200..6484
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507177.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507177.2

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AAGAACTAAG TAAGAAGCTG 
ACAGAGCATG ACCAAGCCAG CAAGGTGCAG CAACAGAAGC TAAAGGCTTT CCAGGCCCAG 
AGAGGTGAAA GCCAGCAAGA GGTCCAGCGC CTCCAGACCC AGCTGAACGA GCTGCAGGCC 
CAGCTGAGCC AGAAGGAGCA GGCAGCTGAG CATTACAAGC TCCAGATGGA GAAAGCCAAG 
ACCCATTATG ATGCCAAGAA GCAGCAGAAC CAAAAGCTGC AGGAACAGCT TCAAGACTTG 
GAGGAACTGC AGAAGGAGAA CAAGGAGCTG CGGTCTGAAG CTGAACGTCT GGGCCGTGAG 
CTGCAGCAGG CAGGGCTGAA GACCAAGGAA GCTGAACAGA CCTGCCGTCA CCTTACAGCC 
CAGGTGCGCA GCCTGGAGGC CCAGGTTGCC CATGCAGACC AGCAGCTTCG TGACTTGGGC 
AAATTCCAGG TGGCAACTGA TGCCTTAAAG AGCCGTGAGC CTCAAGTGAA ACCCCAGCTG 
GACTTAAGTA TTGACAGCCT GGATCTGAGT TTGGAGGAGG GAACCCCATG CAGTGTGGCT 
AGCAAGCTGC CTCGTACCCA ACCAGATGGT ACCAGTGTCC CTGGAGAGCC AGCTTCACCT 
ATCTCCCAGC GCCTGCCCCC CAAAGTGGAA TCCCTGGAGA GCCTCTACTT CACCCCCACT 
CCTGCTCGGG GTCAGGCCCC CTTGGAGACT AGCCTTGACT CTCTGGGGGA TGCCTTCCCT 
GACTCCGGCC GTAAGACCCG CTCTGCTCGC CGCCGTACCA CACAAATCAT CAACATCACT 
ATGACCAAGA AATTAGAATT GGAAGAGCCA GACAGCGCCA ACTCATCCTT CTACAGCACG 
CAGTCTGCTC CTGCTTCCCA GGCCAACTTG CGAGCCACTT CCTCCACTCA GTCTCTGGCC 
CGCTTGGGTT CTCCTGATGA TGGCAACTCC GCTCTGCTTA GCCTGCCTGG CTACCGGCCT 
ACCACACGAA GCTCTGCTCG TCGCTCCCAG GCCAGGATGT CCAGTGGGGC CCCCCAAGGG 
AGGAATAGCT TCTACATGGG CACTTGCCAG GATGAGCCCG AGCAGCTGGA TGATTGGAAC 
CGCATCGCAG AGTTGCAGCA GCGCAACCGA GTATGCCCCC CGCACCTGAA GACCTGCTAC 
CCTCTGGAGT CCAGGCCTAC CCTGAGCCTG GCCACCATCA CTGATGAGGA GATGAAGACT 
GGAGACCCCA GGGAAACCTT GCGTAGAGCC AGCATGCAGC CAGCTCAGAT TGCTGAGGGT 
GTTGGCATAA CTACCAGGCA GCAGCGCAAA CGAGTTTCCT CTGAGACCCA CCAGGGCCCT 
GGCACTCCTG AGTCCAAGAA AGCTACCAGC TGCTTTCCAC GCCCCATGAC CCCCAGGGAC 
CGGCATGAAG GACGTAAGCA GAGCAGCACT GCTGACACCC AGAAGAAAGC AGCTCCAGTT 
CTCAAACAGG CGGACCGGCG TCAGTCAATG GCTTTCAGCA TCCTTAACAC ACCTAAGAAG 
CTGGGAAATA GTCTTCTAAG GCGAGGAGCT TCAAAGAAGA CCCCAGCAAA GGTCTCCCCC 
AATCCTCGCA GTGGAACCCG CCGTTCTCCA CGCATTGCCA CCACCACAAC TGGCACTGCT 
ACTGTTGCCA CCACTCCTCG GGCCAAGGGC AAGGTGAAGC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_006507171.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507234.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X3, mRNA.

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CloneID	OMu39199
Clone ID Related Accession (Same CDS sequence)	XM_006507171.3 , XM_006507174.2 , XM_030241948.1 , XM_006507175.2 , XM_006507171.4 , XM_006507173.3 , XM_006507175.3 , XM_006507173.2 , XM_006507172.3 , XM_006507169.3 , XM_006507170.3 , XM_006507172.4 , XM_006507169.2 , XM_006507170.2 , XM_006507174.1
Accession Version	XM_006507171.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507171.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507234.1
CDS	204..6530
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507171.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507171.3

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_006507175.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507238.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X7, mRNA.

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CloneID	OMu39199
Clone ID Related Accession (Same CDS sequence)	XM_006507171.3 , XM_006507174.2 , XM_030241948.1 , XM_006507175.2 , XM_006507171.4 , XM_006507173.3 , XM_006507175.3 , XM_006507173.2 , XM_006507172.3 , XM_006507169.3 , XM_006507170.3 , XM_006507172.4 , XM_006507169.2 , XM_006507170.2 , XM_006507174.1
Accession Version	XM_006507175.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006507175.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507238.1
CDS	330..6656
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507175.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507175.2

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_006507173.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507236.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X4, mRNA.

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CloneID	OMu39199
Clone ID Related Accession (Same CDS sequence)	XM_006507171.3 , XM_006507174.2 , XM_030241948.1 , XM_006507175.2 , XM_006507171.4 , XM_006507173.3 , XM_006507175.3 , XM_006507173.2 , XM_006507172.3 , XM_006507169.3 , XM_006507170.3 , XM_006507172.4 , XM_006507169.2 , XM_006507170.2 , XM_006507174.1
Accession Version	XM_006507173.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507173.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507236.1
CDS	342..6668
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507173.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507173.2

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_006507172.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507235.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X5, mRNA.

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CloneID	OMu39199
Clone ID Related Accession (Same CDS sequence)	XM_006507171.3 , XM_006507174.2 , XM_030241948.1 , XM_006507175.2 , XM_006507171.4 , XM_006507173.3 , XM_006507175.3 , XM_006507173.2 , XM_006507172.3 , XM_006507169.3 , XM_006507170.3 , XM_006507172.4 , XM_006507169.2 , XM_006507170.2 , XM_006507174.1
Accession Version	XM_006507172.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507172.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507235.1
CDS	403..6729
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507172.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507172.3

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_006507169.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507232.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X1, mRNA.

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CloneID	OMu39199
Clone ID Related Accession (Same CDS sequence)	XM_006507171.3 , XM_006507174.2 , XM_030241948.1 , XM_006507175.2 , XM_006507171.4 , XM_006507173.3 , XM_006507175.3 , XM_006507173.2 , XM_006507172.3 , XM_006507169.3 , XM_006507170.3 , XM_006507172.4 , XM_006507169.2 , XM_006507170.2 , XM_006507174.1
Accession Version	XM_006507169.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507169.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507232.1
CDS	200..6526
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507169.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507169.2

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_006507170.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507233.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X2, mRNA.

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CloneID	OMu39199
Clone ID Related Accession (Same CDS sequence)	XM_006507171.3 , XM_006507174.2 , XM_030241948.1 , XM_006507175.2 , XM_006507171.4 , XM_006507173.3 , XM_006507175.3 , XM_006507173.2 , XM_006507172.3 , XM_006507169.3 , XM_006507170.3 , XM_006507172.4 , XM_006507169.2 , XM_006507170.2 , XM_006507174.1
Accession Version	XM_006507170.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006507170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507233.1
CDS	307..6633
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507170.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507170.2

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_006507174.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507237.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X6, mRNA.

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CloneID	OMu39199
Clone ID Related Accession (Same CDS sequence)	XM_006507171.3 , XM_006507174.2 , XM_030241948.1 , XM_006507175.2 , XM_006507171.4 , XM_006507173.3 , XM_006507175.3 , XM_006507173.2 , XM_006507172.3 , XM_006507169.3 , XM_006507170.3 , XM_006507172.4 , XM_006507169.2 , XM_006507170.2 , XM_006507174.1
Accession Version	XM_006507174.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507237.1
CDS	158..6484
Translation	MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQKNRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507174.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507174.1

ORF Insert Sequence:

ATGACACTCC ATGCCACCCG GGCAGCTACG CTCCTTTCTT GGGTGAACAG TCTGCATGTT 
GCTGACCCCG TGGAGACAGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATTATT 
AACACCATCC ATGACACTAA AGAGGGGCAA CAAATCCTAC AGCAGCCACT ACCGGAAAGA 
CTAGACTTTG TTTGCAGTTT TCTACAGAAA AATCGGAAAC ATCCTTCATC TACACAATGC 
CTGGTATCTG TGCAGAAAGT GATAGAGGGA TCCGAGATGG AACTGGCCAA GATGATAATG 
CTGTTTTTAT ATCAGTCCAC CATGAGCTCC AGAAACCTCC GGGACTGGGA GCAGTTTGAA 
TATGGGGTTC AGGCTGAGTT AGCGGTCATC CTGAAGTTTA TGCTGGATCA TGAGGAGTCG 
CTAAACCTTA CTGAGGACTT AGAGAGTTTC TTGGAGAAAG TTCCTTACAC CCATGCCAGC 
ACCCTCTCAG AAGAGCTTTC TCCACCCAGC CACCAGACCA AGAGGAAGAT TCGCTTCCTA 
GAAATACAGA GGATTGCTTC ATCTTCCAGT GAGAACAACT TCCTCTCAGG CTCTCCATCC 
TCCCCTATGG GCGACATACT GCAGACCCCA CAGTTCCAGA TGAGACGACT GAAGAAGCAG 
CTGGCAGACG AGAGGAGCAA CAGAGATGAC CTGGAGCTGG AGCTGTCTGA GAGCCTCAAG 
CTCCTCACTG AGAAGGACGC GCAGATCGCC ATGATGCAGC AGCGCATTGA TCACCTGGCT 
CTGCTGAATG AGAAGCAGGC AGCCAGCTCC CAGGAGCCCA GTGAGCTTGA GGAGCTCCGA 
GGCAAGAATG AAAGCCTCAC TGTGCGGCTG CATGAGACTT TGAAGCAGTG TCAGAACCTG 
AAGACCGAGA AGAGCCAGAT GGATCGAAAG ATTAGCCAGC TTTCTGAGGA GAATGGGGAC 
CTTTCCTTTA AGGTTCGAGA GTTTGCAAAC CACTTGCAAC AACTTCAGGG TGCATTCAAC 
GACCTGATAG AGGAGCACAG CAAGGCTTCT CAGGAGTGGG CAGAGAAGCA GGCTCGTCTG 
GAAAATGAGC TCAGCACAGC CCTCCAGGAT AAGAAATGTC TTGAAGAGAA GAATGAAATT 
CTTCAGGGAA AGCTTTCCCA GCTGGAAGAT CAAGCAACTC GGCTGCAGGA GAGCCCAGCC 
CCGGAGAAGG GTGAGGTGCT AGGAGACGCC TTGCAGCTGG ACACTCTAAA GCAAGAGGCA 
GCCAAACTTG CCACAGACAA CACCCAGCTC CAAACCCGCG TAGAGACACT GGAGTGTGAA 
CGTGGCAAGC AGGAAGCCCA GCTGCTTGCT GAACGGAGCC GCTTTGAAGA CGAAAAGCAG 
CAGCTGGCCA GCTTGATTGC TGACCTTCAG AGCTCCGTCT CTAACCTCAG CCAGGCCAAA 
GAGGAGTTAG AGCAAGCCTC CCAGGCCCAA GGGGCTCAGC TCACTGCCCA GTTGACCTCT 
ATGACAGGGC TCAATGCCAC CCTGCAGCAG CGAGATCAAG AGCTGGCCAG TCTGAAAGAA 
CAGGCCAAAA AGGAACAGGC TCAGATGCTA CAGACCATGC AAGAGCAAGA ACAGGCTGCC 
CAGGGCCTCC GCCAGCAGGT GGAGCAGCTG AGCAGCAGCC TGAAGCTGAA GGAGCAGCAA 
TTAGAAGAGG CAGCCAAGGA GCAGGAGGCA ACCAGACAGG ATCACGCCCA GCAACTGGCC 
ATCGTTGCTG AGGCTCGAGA GGCCTCTCTA AGGGAACGGG ATACTGCTCG CCAGCAGCTA 
GAAACAGTGG AGAAGGAGAA GGATGCCAAG CTAGAGAGTC TGCAGCAGCA ACTTCAGGCT 
GCCAATGATG CTAGGGACAA TGCGCAGACC TCGGTCACAC AGGCTCAGCA GGAAAAGGCA 
GAGCTGAGCC AAAAGATAGG AGAGCTTCAT GCCTGCATTG AAGCTTCCCA CCAGGAACAG 
CGTCAGGTTC AAGCTCGTGT GACAGAGCTA GAAGCCCAGC TGAAAGCTGA GCAGCAAAAA 
ACAACTGAGC GAGAAAAGGT GGTTCAGGAG AAGGCCCAGC TCCAGGAGCA GCTCCGGGCC 
CTTGAGGAGT CTTTGAAGAT TACCAAAGGC AGCCTTGAAG AAGAGAAACG CAGGGCTGCA 
GATGCCCTGA AGGAGCAGCA GTGCCGTGCC ACTGAGATGG AGGCAGAGTC TCGAAGCCTG 
ATGGAGCAGC GGGAGCGGGA ACAGAAGGAG TTAGAGCAAG AGAAGGCTGG GCGAAAGGGG 
CTGGAGGCCC GGATACAGCA GCTTGAAGAG GCTCATCAGG CTGAGACTGA AGCCTTGCGG 
CATGAGCTGG CAGAGGCCAC AGCTTCCCAG CACAGAGCTG AGAGTGAGTG TGAGCGGCTC 
ATCAGAGAGG TTGAGAGCCG GCAGAAGCGG TTTGAAGCTA GACAGCAAGA GGAGGCAAGG 
TACGGTGCCA TGTTCCAGGA ACAGCTGATG GCTTTGAAAG GGGAGAAGAC TGGCCAAGAG 
GTCCAGGAGG AGGCAGTAGA GATCCACAGT GAAGGCCAGC CAGGCCAGCA GCAGAGTCAG 
CTAGCCCAGC TTCATGCCAG TCTGGCCAAA GCCATCCAGC AGGTCCAGGA GAAGGAAGTG 
AGAGCACAGA AGCTGGTAGA TGATCTCTCA GCTCTGCAGG AGAAGATGGC TGCCACTAAC 
AAGGAGGTGG CCTGTCTGAA GACCTTGGTG CTCAAGGCAG GTGAGCAGCA GGAAACAGCC 
TCACTTGAGC TGCTCAAAGA GCCCCCGAGA GCTGCAAACA GAGCGTCTGA CCAGCTAGGA 
GAACAGCAGG GACGGCCATT CTCTAGTACA CACGCAGCAG TAAAAGCTAT GGAGCGTGAA 
GCTGAGCAGA TGGGCGGTGA ACTGGAGAGG CTGCGGGCAG CACTGATCAA GAGCCAGGGC 
CAGCAGCAAG AGGAGCGTGG GCAACAGGAG AGGGAGGTAG CACGGCTGAC CCAGGAGCGG 
GGCCAGGCCC AGGCTGACCT GGCTCAGGAG AAGGCAGCCA AGGCAGAGCT GGAGATGCGA 
CTGCAGAACA CCCTCAATGA GCAGCGTGTG GAGTTTGCTG CCCTACAGGA AGCACTGGCC 
CATGCCCTGA CAGAAAAGGA AGGGACAGAC CAAGAGCTTG CCAAACTTCG AGGGCAGGAG 
GCAGCTCAAA GAACAGAGCT GAAGGAGCTT CAACAGACTT TGGAACAGCT GAAAATACAG 
TTAGTGAAGA AAGAAAAAGA GCACCCTGCA GGGGGTGCCA GTGGAGAAGA TGCTTCTGGC 
CCAGGAACCC AGTCGGAGAC AGCTGGAAAG ACAGATGCAC CAGGCCCTGA GTTGCAGGCA 
CTTCGGGCAG AGATCAGTAA GCTAGAGCAG CAGTGTCAGC AGCAGCAGCA ACAGGTGGAA 
GGCCTGACAC ACAGCCTCAA GTCTGAGCGG GCTTGCCGGG CCGAGCAAGA CAAAGCCCTG 
GAGACACTGC AGGGCCAGTT AGAAGAGAAG GCTCGAGAGC TAGGGCATAA TCAGGCTGCC 
TCAGCTTCAG CTCAGAGAGA GTTGCAAGCC CTCCGTGCTA AAGCCCAGGA CCATAGCAAA 
GCTGAGGAAG AGTGGAAGGC CCAGGTAGCC CGGGGCCAGC AGGAGGCTGA AAGAAAAAGC 
AGTCTTATCA GCAGCTTGGA AGAGGAGGTA TCCATCCTGA ATCGCCAAGT CCTAGAGAAG 
GAAGGGGAGA GCAAGGAGTT GAAGCGCCTG GTTGTAGCTG AATCCGAGAA GAGCCAGAAG 
CTGGAAGAGA GGCTGCGCCT GCTTCAAGTA GAAACAGCCA GCAATAGTGC CAGGGCAGCA 
GAACGCAGCT CAGCTCTACG AGAGGAGGTC CAGAGCCTCC GGGAAGAGGT GGAGAAACAG 
CGTGTAGTTT CAGAGAACTC GCGGCAGGAG TTGGCCTCAC AAGCAGAGCG AGCTGAGGAG 
TTGGGCCAAG AATTGAAGGC CTGGCAGGAG AAGTTTTTCC AGAAGGAGCA AGCTCTCTCT 
GCCCTGCAGC TGGAGCACAC CAGCACACAG GCCCTGGTAA GTGAGCTGCT GCCTGCCAAG 
CACCTTTGTC AGCAGCTGCA AGCTGAGCAA GCGGCTGCCG AGAAGCGCTT CCGGGAGGAG 
CTCGAGCAGA GCAAGCAGGC AGCTGGTGGG CTCCAGGCAG AGCTGATGCG GGCCCAGCGG 
GAGCTCGGAG AGCTCGGATC TTTGCGGCAG AAGATAGTTG AGCAGGAGCG AGCAGCCCAG 
CAGCTGCGGG CAGAGAAGGC CAGCTATGCA GAGCAGCTGA GCATGCTGAA GAAGGCCCAT 
GGCCTGCTGG CAGAGGAGAA CCGGGGGCTG GGAGAGCGGG CCAACCTTGG CCGGCAGTTT 
CTGGAAGTAG AACTAGATCA AGCCCGGGAG AAATATGTCC AGGAGTTGGC TGCTGTGCGT 
ACTGATGCTG AGACCCACTT GGCTGAAATG CGACAAGAAG CACAGAGTAC TAGCCGGGAG 
CTGGAGGTGA TGACTGCCAA GTACGAAGGT GCCAAGGTGA AGGTCCTAGA GGAAAGGCAG 
AGGTTCCAAG AAGAGAGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGTATTTCAA 
AGAGAGCAGA CTAAGCAGGT GGAAGAACTA AGTAAGAAGC TGACAGAGCA TGACCAAGCC 
AGCAAGGTGC AGCAACAGAA GCTAAAGGCT TTCCAGGCCC AGAGAGGTGA AAGCCAGCAA 
GAGGTCCAGC GCCTCCAGAC CCAGCTGAAC GAGCTGCAGG CCCAGCTGAG CCAGAAGGAG 
CAGGCAGCTG AGCATTACAA GCTCCAGATG GAGAAAGCCA AGACCCATTA TGATGCCAAG 
AAGCAGCAGA ACCAAAAGCT GCAGGAACAG CTTCAAGACT TGGAGGAACT GCAGAAGGAG 
AACAAGGAGC TGCGGTCTGA AGCTGAACGT CTGGGCCGTG AGCTGCAGCA GGCAGGGCTG 
AAGACCAAGG AAGCTGAACA GACCTGCCGT CACCTTACAG CCCAGGTGCG CAGCCTGGAG 
GCCCAGGTTG CCCATGCAGA CCAGCAGCTT CGTGACTTGG GCAAATTCCA GGTGGCAACT 
GATGCCTTAA AGAGCCGTGA GCCTCAAGTG AAACCCCAGC TGGACTTAAG TATTGACAGC 
CTGGATCTGA GTTTGGAGGA GGGAACCCCA TGCAGTGTGG CTAGCAAGCT GCCTCGTACC 
CAACCAGATG GTACCAGTGT CCCTGGAGAG CCAGCTTCAC CTATCTCCCA GCGCCTGCCC 
CCCAAAGTGG AATCCCTGGA GAGCCTCTAC TTCACCCCCA CTCCTGCTCG GGGTCAGGCC 
CCCTTGGAGA CTAGCCTTGA CTCTCTGGGG GATGCCTTCC CTGACTCCGG CCGTAAGACC 
CGCTCTGCTC GCCGCCGTAC CACACAAATC ATCAACATCA CTATGACCAA GAAATTAGAA 
TTGGAAGAGC CAGACAGCGC CAACTCATCC TTCTACAGCA CGCAGTCTGC TCCTGCTTCC 
CAGGCCAACT TGCGAGCCAC TTCCTCCACT CAGTCTCTGG CCCGCTTGGG TTCTCCTGAT 
GATGGCAACT CCGCTCTGCT TAGCCTGCCT GGCTACCGGC CTACCACACG AAGCTCTGCT 
CGTCGCTCCC AGGCCAGGAT GTCCAGTGGG GCCCCCCAAG GGAGGAATAG CTTCTACATG 
GGCACTTGCC AGGATGAGCC CGAGCAGCTG GATGATTGGA ACCGCATCGC AGAGTTGCAG 
CAGCGCAACC GAGTATGCCC CCCGCACCTG AAGACCTGCT ACCCTCTGGA GTCCAGGCCT 
ACCCTGAGCC TGGCCACCAT CACTGATGAG GAGATGAAGA CTGGAGACCC CAGGGAAACC 
TTGCGTAGAG CCAGCATGCA GCCAGCTCAG ATTGCTGAGG GTGTTGGCAT AACTACCAGG 
CAGCAGCGCA AACGAGTTTC CTCTGAGACC CACCAGGGCC CTGGCACTCC TGAGTCCAAG 
AAAGCTACCA GCTGCTTTCC ACGCCCCATG ACCCCCAGGG ACCGGCATGA AGGACGTAAG 
CAGAGCAGCA CTGCTGACAC CCAGAAGAAA GCAGCTCCAG TTCTCAAACA GGCGGACCGG 
CGTCAGTCAA TGGCTTTCAG CATCCTTAAC ACACCTAAGA AGCTGGGAAA TAGTCTTCTA 
AGGCGAGGAG CTTCAAAGAA GACCCCAGCA AAGGTCTCCC CCAATCCTCG CAGTGGAACC 
CGCCGTTCTC CACGCATTGC CACCACCACA ACTGGCACTG CTACTGTTGC CACCACTCCT 
CGGGCCAAGG GCAAGGTGAA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Numa1 ( XM_006507178.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507241.1 Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X9, mRNA.

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CloneID	OMu39201
Clone ID Related Accession (Same CDS sequence)	XM_030241949.1 , XM_006507178.2
Accession Version	XM_006507178.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nuclear mitotic apparatus protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006507178.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCAT TGGGTGGCCC CAAAGCCGAG CTGACTTCAG ATGGGAATAG AAAGAAGGCA 
GAAAATCGGA AACATCCTTC ATCTACACAA TGCCTGGTAT CTGTGCAGAA AGTGATAGAG 
GGATCCGAGA TGGAACTGGC CAAGATGATA ATGCTGTTTT TATATCAGTC CACCATGAGC 
TCCAGAAACC TCCGGGACTG GGAGCAGTTT GAATATGGGG TTCAGGCTGA GTTAGCGGTC 
ATCCTGAAGT TTATGCTGGA TCATGAGGAG TCGCTAAACC TTACTGAGGA CTTAGAGAGT 
TTCTTGGAGA AAGTTCCTTA CACCCATGCC AGCACCCTCT CAGAAGAGCT TTCTCCACCC 
AGCCACCAGA CCAAGAGGAA GATTCGCTTC CTAGAAATAC AGAGGATTGC TTCATCTTCC 
AGTGAGAACA ACTTCCTCTC AGGCTCTCCA TCCTCCCCTA TGGGCGACAT ACTGCAGACC 
CCACAGTTCC AGATGAGACG ACTGAAGAAG CAGCTGGCAG ACGAGAGGAG CAACAGAGAT 
GACCTGGAGC TGGAGCTGTC TGAGAGCCTC AAGCTCCTCA CTGAGAAGGA CGCGCAGATC 
GCCATGATGC AGCAGCGCAT TGATCACCTG GCTCTGCTGA ATGAGAAGCA GGCAGCCAGC 
TCCCAGGAGC CCAGTGAGCT TGAGGAGCTC CGAGGCAAGA ATGAAAGCCT CACTGTGCGG 
CTGCATGAGA CTTTGAAGCA GTGTCAGAAC CTGAAGACCG AGAAGAGCCA GATGGATCGA 
AAGATTAGCC AGCTTTCTGA GGAGAATGGG GACCTTTCCT TTAAGGTTCG AGAGTTTGCA 
AACCACTTGC AACAACTTCA GGGTGCATTC AACGACCTGA TAGAGGAGCA CAGCAAGGCT 
TCTCAGGAGT GGGCAGAGAA GCAGGCTCGT CTGGAAAATG AGCTCAGCAC AGCCCTCCAG 
GATAAGAAAT GTCTTGAAGA GAAGAATGAA ATTCTTCAGG GAAAGCTTTC CCAGCTGGAA 
GATCAAGCAA CTCGGCTGCA GGAGAGCCCA GCCCCGGAGA AGGGTGAGGT GCTAGGAGAC 
GCCTTGCAGC TGGACACTCT AAAGCAAGAG GCAGCCAAAC TTGCCACAGA CAACACCCAG 
CTCCAAACCC GCGTAGAGAC ACTGGAGTGT GAACGTGGCA AGCAGGAAGC CCAGCTGCTT 
GCTGAACGGA GCCGCTTTGA AGACGAAAAG CAGCAGCTGG CCAGCTTGAT TGCTGACCTT 
CAGAGCTCCG TCTCTAACCT CAGCCAGGCC AAAGAGGAGT TAGAGCAAGC CTCCCAGGCC 
CAAGGGGCTC AGCTCACTGC CCAGTTGACC TCTATGACAG GGCTCAATGC CACCCTGCAG 
CAGCGAGATC AAGAGCTGGC CAGTCTGAAA GAACAGGCCA AAAAGGAACA GGCTCAGATG 
CTACAGACCA TGCAAGAGCA AGAACAGGCT GCCCAGGGCC TCCGCCAGCA GGTGGAGCAG 
CTGAGCAGCA GCCTGAAGCT GAAGGAGCAG CAATTAGAAG AGGCAGCCAA GGAGCAGGAG 
GCAACCAGAC AGGATCACGC CCAGCAACTG GCCATCGTTG CTGAGGCTCG AGAGGCCTCT 
CTAAGGGAAC GGGATACTGC TCGCCAGCAG CTAGAAACAG TGGAGAAGGA GAAGGATGCC 
AAGCTAGAGA GTCTGCAGCA GCAACTTCAG GCTGCCAATG ATGCTAGGGA CAATGCGCAG 
ACCTCGGTCA CACAGGCTCA GCAGGAAAAG GCAGAGCTGA GCCAAAAGAT AGGAGAGCTT 
CATGCCTGCA TTGAAGCTTC CCACCAGGAA CAGCGTCAGG TTCAAGCTCG TGTGACAGAG 
CTAGAAGCCC AGCTGAAAGC TGAGCAGCAA AAAACAACTG AGCGAGAAAA GGTGGTTCAG 
GAGAAGGCCC AGCTCCAGGA GCAGCTCCGG GCCCTTGAGG AGTCTTTGAA GATTACCAAA 
GGCAGCCTTG AAGAAGAGAA ACGCAGGGCT GCAGATGCCC TGAAGGAGCA GCAGTGCCGT 
GCCACTGAGA TGGAGGCAGA GTCTCGAAGC CTGATGGAGC AGCGGGAGCG GGAACAGAAG 
GAGTTAGAGC AAGAGAAGGC TGGGCGAAAG GGGCTGGAGG CCCGGATACA GCAGCTTGAA 
GAGGCTCATC AGGCTGAGAC TGAAGCCTTG CGGCATGAGC TGGCAGAGGC CACAGCTTCC 
CAGCACAGAG CTGAGAGTGA GTGTGAGCGG CTCATCAGAG AGGTTGAGAG CCGGCAGAAG 
CGGTTTGAAG CTAGACAGCA AGAGGAGGCA AGGTACGGTG CCATGTTCCA GGAACAGCTG 
ATGGCTTTGA AAGGGGAGAA GACTGGCCAA GAGGTCCAGG AGGAGGCAGT AGAGATCCAC 
AGTGAAGGCC AGCCAGGCCA GCAGCAGAGT CAGCTAGCCC AGCTTCATGC CAGTCTGGCC 
AAAGCCATCC AGCAGGTCCA GGAGAAGGAA GTGAGAGCAC AGAAGCTGGT AGATGATCTC 
TCAGCTCTGC AGGAGAAGAT GGCTGCCACT AACAAGGAGG TGGCCTGTCT GAAGACCTTG 
GTGCTCAAGG CAGGTGAGCA GCAGGAAACA GCCTCACTTG AGCTGCTCAA AGAGCCCCCG 
AGAGCTGCAA ACAGAGCGTC TGACCAGCTA GGAGAACAGC AGGGACGGCC ATTCTCTAGT 
ACACACGCAG CAGTAAAAGC TATGGAGCGT GAAGCTGAGC AGATGGGCGG TGAACTGGAG 
AGGCTGCGGG CAGCACTGAT CAAGAGCCAG GGCCAGCAGC AAGAGGAGCG TGGGCAACAG 
GAGAGGGAGG TAGCACGGCT GACCCAGGAG CGGGGCCAGG CCCAGGCTGA CCTGGCTCAG 
GAGAAGGCAG CCAAGGCAGA GCTGGAGATG CGACTGCAGA ACACCCTCAA TGAGCAGCGT 
GTGGAGTTTG CTGCCCTACA GGAAGCACTG GCCCATGCCC TGACAGAAAA GGAAGGGACA 
GACCAAGAGC TTGCCAAACT TCGAGGGCAG GAGGCAGCTC AAAGAACAGA GCTGAAGGAG 
CTTCAACAGA CTTTGGAACA GCTGAAAATA CAGTTAGTGA AGAAAGAAAA AGAGCACCCT 
GCAGGGGGTG CCAGTGGAGA AGATGCTTCT GGCCCAGGAA CCCAGTCGGA GACAGCTGGA 
AAGACAGATG CACCAGGCCC TGAGTTGCAG GCACTTCGGG CAGAGATCAG TAAGCTAGAG 
CAGCAGTGTC AGCAGCAGCA GCAACAGGTG GAAGGCCTGA CACACAGCCT CAAGTCTGAG 
CGGGCTTGCC GGGCCGAGCA AGACAAAGCC CTGGAGACAC TGCAGGGCCA GTTAGAAGAG 
AAGGCTCGAG AGCTAGGGCA TAATCAGGCT GCCTCAGCTT CAGCTCAGAG AGAGTTGCAA 
GCCCTCCGTG CTAAAGCCCA GGACCATAGC AAAGCTGAGG AAGAGTGGAA GGCCCAGGTA 
GCCCGGGGCC AGCAGGAGGC TGAAAGAAAA AGCAGTCTTA TCAGCAGCTT GGAAGAGGAG 
GTATCCATCC TGAATCGCCA AGTCCTAGAG AAGGAAGGGG AGAGCAAGGA GTTGAAGCGC 
CTGGTTGTAG CTGAATCCGA GAAGAGCCAG AAGCTGGAAG AGAGGCTGCG CCTGCTTCAA 
GTAGAAACAG CCAGCAATAG TGCCAGGGCA GCAGAACGCA GCTCAGCTCT ACGAGAGGAG 
GTCCAGAGCC TCCGGGAAGA GGTGGAGAAA CAGCGTGTAG TTTCAGAGAA CTCGCGGCAG 
GAGTTGGCCT CACAAGCAGA GCGAGCTGAG GAGTTGGGCC AAGAATTGAA GGCCTGGCAG 
GAGAAGTTTT TCCAGAAGGA GCAAGCTCTC TCTGCCCTGC AGCTGGAGCA CACCAGCACA 
CAGGCCCTGG TAAGTGAGCT GCTGCCTGCC AAGCACCTTT GTCAGCAGCT GCAAGCTGAG 
CAAGCGGCTG CCGAGAAGCG CTTCCGGGAG GAGCTCGAGC AGAGCAAGCA GGCAGCTGGT 
GGGCTCCAGG CAGAGCTGAT GCGGGCCCAG CGGGAGCTCG GAGAGCTCGG ATCTTTGCGG 
CAGAAGATAG TTGAGCAGGA GCGAGCAGCC CAGCAGCTGC GGGCAGAGAA GGCCAGCTAT 
GCAGAGCAGC TGAGCATGCT GAAGAAGGCC CATGGCCTGC TGGCAGAGGA GAACCGGGGG 
CTGGGAGAGC GGGCCAACCT TGGCCGGCAG TTTCTGGAAG TAGAACTAGA TCAAGCCCGG 
GAGAAATATG TCCAGGAGTT GGCTGCTGTG CGTACTGATG CTGAGACCCA CTTGGCTGAA 
ATGCGACAAG AAGCACAGAG TACTAGCCGG GAGCTGGAGG TGATGACTGC CAAGTACGAA 
GGTGCCAAGG TGAAGGTCCT AGAGGAAAGG CAGAGGTTCC AAGAAGAGAG GCAGAAACTC 
ACTGCCCAGG TGGAGCAACT AGAGGTATTT CAAAGAGAGC AGACTAAGCA GGTGGAAGAA 
CTAAGTAAGA AGCTGACAGA GCATGACCAA GCCAGCAAGG TGCAGCAACA GAAGCTAAAG 
GCTTTCCAGG CCCAGAGAGG TGAAAGCCAG CAAGAGGTCC AGCGCCTCCA GACCCAGCTG 
AACGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCAG CTGAGCATTA CAAGCTCCAG 
ATGGAGAAAG CCAAGACCCA TTATGATGCC AAGAAGCAGC AGAACCAAAA GCTGCAGGAA 
CAGCTTCAAG ACTTGGAGGA ACTGCAGAAG GAGAACAAGG AGCTGCGGTC TGAAGCTGAA 
CGTCTGGGCC GTGAGCTGCA GCAGGCAGGG CTGAAGACCA AGGAAGCTGA ACAGACCTGC 
CGTCACCTTA CAGCCCAGGT GCGCAGCCTG GAGGCCCAGG TTGCCCATGC AGACCAGCAG 
CTTCGTGACT TGGGCAAATT CCAGGTGGCA ACTGATGCCT TAAAGAGCCG TGAGCCTCAA 
GTGAAACCCC AGCTGGACTT AAGTATTGAC AGCCTGGATC TGAGTTTGGA GGAGGGAACC 
CCATGCAGTG TGGCTAGCAA GCTGCCTCGT ACCCAACCAG ATGGTACCAG TGTCCCTGGA 
GAGCCAGCTT CACCTATCTC CCAGCGCCTG CCCCCCAAAG TGGAATCCCT GGAGAGCCTC 
TACTTCACCC CCACTCCTGC TCGGGGTCAG GCCCCCTTGG AGACTAGCCT TGACTCTCTG 
GGGGATGCCT TCCCTGACTC CGGCCGTAAG ACCCGCTCTG CTCGCCGCCG TACCACACAA 
ATCATCAACA TCACTATGAC CAAGAAATTA GAATTGGAAG AGCCAGACAG CGCCAACTCA 
TCCTTCTACA GCACGCAGTC TGCTCCTGCT TCCCAGGCCA ACTTGCGAGC CACTTCCTCC 
ACTCAGTCTC TGGCCCGCTT GGGTTCTCCT GATGATGGCA ACTCCGCTCT GCTTAGCCTG 
CCTGGCTACC GGCCTACCAC ACGAAGCTCT GCTCGTCGCT CCCAGGCCAG GATGTCCAGT 
GGGGCCCCCC AAGGGAGGAA TAGCTTCTAC ATGGGCACTT GCCAGGATGA GCCCGAGCAG 
CTGGATGATT GGAACCGCAT CGCAGAGTTG CAGCAGCGCA ACCGAGTATG CCCCCCGCAC 
CTGAAGACCT GCTACCCTCT GGAGTCCAGG CCTACCCTGA GCCTGGCCAC CATCACTGAT 
GAGGAGATGA AGACTGGAGA CCCCAGGGAA ACCTTGCGTA GAGCCAGCAT GCAGCCAGCT 
CAGATTGCTG AGGGTGTTGG CATAACTACC AGGCAGCAGC GCAAACGAGT TTCCTCTGAG 
ACCCACCAGG GCCCTGGCAC TCCTGAGTCC AAGAAAGCTA CCAGCTGCTT TCCACGCCCC 
ATGACCCCCA GGGACCGGCA TGAAGGACGT AAGCAGAGCA GCACTGCTGA CACCCAGAAG 
AAAGCAGCTC CAGTTCTCAA ACAGGCGGAC CGGCGTCAGT CAATGGCTTT CAGCATCCTT 
AACACACCTA AGAAGCTGGG AAATAGTCTT CTAAGGCGAG GAGCTTCAAA GAAGACCCCA 
GCAAAGGTCT CCCCCAATCC TCGCAGTGGA ACCCGCCGTT CTCCACGCAT TGCCACCACC 
ACAACTGGCA CTGCTACTGT TGCCACCACT CCTCGGGCCA AGGGCAAGGT GAAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507241.1
CDS	20..6199
Translation	MGALGGPKAELTSDGNRKKAENRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEESLNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTPQFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELRGKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKASQEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEAAKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQGAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQLEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQTSVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRALEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEEAHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQEVQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLVLKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQGQQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTDQELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQALRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQEKFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQRELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLTEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEGVGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSMAFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH

Target ORF information:

RefSeq Version	XM_006507178.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear mitotic apparatus protein 1 (Numa1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507178.2

ORF Insert Sequence:

ATGGGAGCAT TGGGTGGCCC CAAAGCCGAG CTGACTTCAG ATGGGAATAG AAAGAAGGCA 
GAAAATCGGA AACATCCTTC ATCTACACAA TGCCTGGTAT CTGTGCAGAA AGTGATAGAG 
GGATCCGAGA TGGAACTGGC CAAGATGATA ATGCTGTTTT TATATCAGTC CACCATGAGC 
TCCAGAAACC TCCGGGACTG GGAGCAGTTT GAATATGGGG TTCAGGCTGA GTTAGCGGTC 
ATCCTGAAGT TTATGCTGGA TCATGAGGAG TCGCTAAACC TTACTGAGGA CTTAGAGAGT 
TTCTTGGAGA AAGTTCCTTA CACCCATGCC AGCACCCTCT CAGAAGAGCT TTCTCCACCC 
AGCCACCAGA CCAAGAGGAA GATTCGCTTC CTAGAAATAC AGAGGATTGC TTCATCTTCC 
AGTGAGAACA ACTTCCTCTC AGGCTCTCCA TCCTCCCCTA TGGGCGACAT ACTGCAGACC 
CCACAGTTCC AGATGAGACG ACTGAAGAAG CAGCTGGCAG ACGAGAGGAG CAACAGAGAT 
GACCTGGAGC TGGAGCTGTC TGAGAGCCTC AAGCTCCTCA CTGAGAAGGA CGCGCAGATC 
GCCATGATGC AGCAGCGCAT TGATCACCTG GCTCTGCTGA ATGAGAAGCA GGCAGCCAGC 
TCCCAGGAGC CCAGTGAGCT TGAGGAGCTC CGAGGCAAGA ATGAAAGCCT CACTGTGCGG 
CTGCATGAGA CTTTGAAGCA GTGTCAGAAC CTGAAGACCG AGAAGAGCCA GATGGATCGA 
AAGATTAGCC AGCTTTCTGA GGAGAATGGG GACCTTTCCT TTAAGGTTCG AGAGTTTGCA 
AACCACTTGC AACAACTTCA GGGTGCATTC AACGACCTGA TAGAGGAGCA CAGCAAGGCT 
TCTCAGGAGT GGGCAGAGAA GCAGGCTCGT CTGGAAAATG AGCTCAGCAC AGCCCTCCAG 
GATAAGAAAT GTCTTGAAGA GAAGAATGAA ATTCTTCAGG GAAAGCTTTC CCAGCTGGAA 
GATCAAGCAA CTCGGCTGCA GGAGAGCCCA GCCCCGGAGA AGGGTGAGGT GCTAGGAGAC 
GCCTTGCAGC TGGACACTCT AAAGCAAGAG GCAGCCAAAC TTGCCACAGA CAACACCCAG 
CTCCAAACCC GCGTAGAGAC ACTGGAGTGT GAACGTGGCA AGCAGGAAGC CCAGCTGCTT 
GCTGAACGGA GCCGCTTTGA AGACGAAAAG CAGCAGCTGG CCAGCTTGAT TGCTGACCTT 
CAGAGCTCCG TCTCTAACCT CAGCCAGGCC AAAGAGGAGT TAGAGCAAGC CTCCCAGGCC 
CAAGGGGCTC AGCTCACTGC CCAGTTGACC TCTATGACAG GGCTCAATGC CACCCTGCAG 
CAGCGAGATC AAGAGCTGGC CAGTCTGAAA GAACAGGCCA AAAAGGAACA GGCTCAGATG 
CTACAGACCA TGCAAGAGCA AGAACAGGCT GCCCAGGGCC TCCGCCAGCA GGTGGAGCAG 
CTGAGCAGCA GCCTGAAGCT GAAGGAGCAG CAATTAGAAG AGGCAGCCAA GGAGCAGGAG 
GCAACCAGAC AGGATCACGC CCAGCAACTG GCCATCGTTG CTGAGGCTCG AGAGGCCTCT 
CTAAGGGAAC GGGATACTGC TCGCCAGCAG CTAGAAACAG TGGAGAAGGA GAAGGATGCC 
AAGCTAGAGA GTCTGCAGCA GCAACTTCAG GCTGCCAATG ATGCTAGGGA CAATGCGCAG 
ACCTCGGTCA CACAGGCTCA GCAGGAAAAG GCAGAGCTGA GCCAAAAGAT AGGAGAGCTT 
CATGCCTGCA TTGAAGCTTC CCACCAGGAA CAGCGTCAGG TTCAAGCTCG TGTGACAGAG 
CTAGAAGCCC AGCTGAAAGC TGAGCAGCAA AAAACAACTG AGCGAGAAAA GGTGGTTCAG 
GAGAAGGCCC AGCTCCAGGA GCAGCTCCGG GCCCTTGAGG AGTCTTTGAA GATTACCAAA 
GGCAGCCTTG AAGAAGAGAA ACGCAGGGCT GCAGATGCCC TGAAGGAGCA GCAGTGCCGT 
GCCACTGAGA TGGAGGCAGA GTCTCGAAGC CTGATGGAGC AGCGGGAGCG GGAACAGAAG 
GAGTTAGAGC AAGAGAAGGC TGGGCGAAAG GGGCTGGAGG CCCGGATACA GCAGCTTGAA 
GAGGCTCATC AGGCTGAGAC TGAAGCCTTG CGGCATGAGC TGGCAGAGGC CACAGCTTCC 
CAGCACAGAG CTGAGAGTGA GTGTGAGCGG CTCATCAGAG AGGTTGAGAG CCGGCAGAAG 
CGGTTTGAAG CTAGACAGCA AGAGGAGGCA AGGTACGGTG CCATGTTCCA GGAACAGCTG 
ATGGCTTTGA AAGGGGAGAA GACTGGCCAA GAGGTCCAGG AGGAGGCAGT AGAGATCCAC 
AGTGAAGGCC AGCCAGGCCA GCAGCAGAGT CAGCTAGCCC AGCTTCATGC CAGTCTGGCC 
AAAGCCATCC AGCAGGTCCA GGAGAAGGAA GTGAGAGCAC AGAAGCTGGT AGATGATCTC 
TCAGCTCTGC AGGAGAAGAT GGCTGCCACT AACAAGGAGG TGGCCTGTCT GAAGACCTTG 
GTGCTCAAGG CAGGTGAGCA GCAGGAAACA GCCTCACTTG AGCTGCTCAA AGAGCCCCCG 
AGAGCTGCAA ACAGAGCGTC TGACCAGCTA GGAGAACAGC AGGGACGGCC ATTCTCTAGT 
ACACACGCAG CAGTAAAAGC TATGGAGCGT GAAGCTGAGC AGATGGGCGG TGAACTGGAG 
AGGCTGCGGG CAGCACTGAT CAAGAGCCAG GGCCAGCAGC AAGAGGAGCG TGGGCAACAG 
GAGAGGGAGG TAGCACGGCT GACCCAGGAG CGGGGCCAGG CCCAGGCTGA CCTGGCTCAG 
GAGAAGGCAG CCAAGGCAGA GCTGGAGATG CGACTGCAGA ACACCCTCAA TGAGCAGCGT 
GTGGAGTTTG CTGCCCTACA GGAAGCACTG GCCCATGCCC TGACAGAAAA GGAAGGGACA 
GACCAAGAGC TTGCCAAACT TCGAGGGCAG GAGGCAGCTC AAAGAACAGA GCTGAAGGAG 
CTTCAACAGA CTTTGGAACA GCTGAAAATA CAGTTAGTGA AGAAAGAAAA AGAGCACCCT 
GCAGGGGGTG CCAGTGGAGA AGATGCTTCT GGCCCAGGAA CCCAGTCGGA GACAGCTGGA 
AAGACAGATG CACCAGGCCC TGAGTTGCAG GCACTTCGGG CAGAGATCAG TAAGCTAGAG 
CAGCAGTGTC AGCAGCAGCA GCAACAGGTG GAAGGCCTGA CACACAGCCT CAAGTCTGAG 
CGGGCTTGCC GGGCCGAGCA AGACAAAGCC CTGGAGACAC TGCAGGGCCA GTTAGAAGAG 
AAGGCTCGAG AGCTAGGGCA TAATCAGGCT GCCTCAGCTT CAGCTCAGAG AGAGTTGCAA 
GCCCTCCGTG CTAAAGCCCA GGACCATAGC AAAGCTGAGG AAGAGTGGAA GGCCCAGGTA 
GCCCGGGGCC AGCAGGAGGC TGAAAGAAAA AGCAGTCTTA TCAGCAGCTT GGAAGAGGAG 
GTATCCATCC TGAATCGCCA AGTCCTAGAG AAGGAAGGGG AGAGCAAGGA GTTGAAGCGC 
CTGGTTGTAG CTGAATCCGA GAAGAGCCAG AAGCTGGAAG AGAGGCTGCG CCTGCTTCAA 
GTAGAAACAG CCAGCAATAG TGCCAGGGCA GCAGAACGCA GCTCAGCTCT ACGAGAGGAG 
GTCCAGAGCC TCCGGGAAGA GGTGGAGAAA CAGCGTGTAG TTTCAGAGAA CTCGCGGCAG 
GAGTTGGCCT CACAAGCAGA GCGAGCTGAG GAGTTGGGCC AAGAATTGAA GGCCTGGCAG 
GAGAAGTTTT TCCAGAAGGA GCAAGCTCTC TCTGCCCTGC AGCTGGAGCA CACCAGCACA 
CAGGCCCTGG TAAGTGAGCT GCTGCCTGCC AAGCACCTTT GTCAGCAGCT GCAAGCTGAG 
CAAGCGGCTG CCGAGAAGCG CTTCCGGGAG GAGCTCGAGC AGAGCAAGCA GGCAGCTGGT 
GGGCTCCAGG CAGAGCTGAT GCGGGCCCAG CGGGAGCTCG GAGAGCTCGG ATCTTTGCGG 
CAGAAGATAG TTGAGCAGGA GCGAGCAGCC CAGCAGCTGC GGGCAGAGAA GGCCAGCTAT 
GCAGAGCAGC TGAGCATGCT GAAGAAGGCC CATGGCCTGC TGGCAGAGGA GAACCGGGGG 
CTGGGAGAGC GGGCCAACCT TGGCCGGCAG TTTCTGGAAG TAGAACTAGA TCAAGCCCGG 
GAGAAATATG TCCAGGAGTT GGCTGCTGTG CGTACTGATG CTGAGACCCA CTTGGCTGAA 
ATGCGACAAG AAGCACAGAG TACTAGCCGG GAGCTGGAGG TGATGACTGC CAAGTACGAA 
GGTGCCAAGG TGAAGGTCCT AGAGGAAAGG CAGAGGTTCC AAGAAGAGAG GCAGAAACTC 
ACTGCCCAGG TGGAGCAACT AGAGGTATTT CAAAGAGAGC AGACTAAGCA GGTGGAAGAA 
CTAAGTAAGA AGCTGACAGA GCATGACCAA GCCAGCAAGG TGCAGCAACA GAAGCTAAAG 
GCTTTCCAGG CCCAGAGAGG TGAAAGCCAG CAAGAGGTCC AGCGCCTCCA GACCCAGCTG 
AACGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCAG CTGAGCATTA CAAGCTCCAG 
ATGGAGAAAG CCAAGACCCA TTATGATGCC AAGAAGCAGC AGAACCAAAA GCTGCAGGAA 
CAGCTTCAAG ACTTGGAGGA ACTGCAGAAG GAGAACAAGG AGCTGCGGTC TGAAGCTGAA 
CGTCTGGGCC GTGAGCTGCA GCAGGCAGGG CTGAAGACCA AGGAAGCTGA ACAGACCTGC 
CGTCACCTTA CAGCCCAGGT GCGCAGCCTG GAGGCCCAGG TTGCCCATGC AGACCAGCAG 
CTTCGTGACT TGGGCAAATT CCAGGTGGCA ACTGATGCCT TAAAGAGCCG TGAGCCTCAA 
GTGAAACCCC AGCTGGACTT AAGTATTGAC AGCCTGGATC TGAGTTTGGA GGAGGGAACC 
CCATGCAGTG TGGCTAGCAA GCTGCCTCGT ACCCAACCAG ATGGTACCAG TGTCCCTGGA 
GAGCCAGCTT CACCTATCTC CCAGCGCCTG CCCCCCAAAG TGGAATCCCT GGAGAGCCTC 
TACTTCACCC CCACTCCTGC TCGGGGTCAG GCCCCCTTGG AGACTAGCCT TGACTCTCTG 
GGGGATGCCT TCCCTGACTC CGGCCGTAAG ACCCGCTCTG CTCGCCGCCG TACCACACAA 
ATCATCAACA TCACTATGAC CAAGAAATTA GAATTGGAAG AGCCAGACAG CGCCAACTCA 
TCCTTCTACA GCACGCAGTC TGCTCCTGCT TCCCAGGCCA ACTTGCGAGC CACTTCCTCC 
ACTCAGTCTC TGGCCCGCTT GGGTTCTCCT GATGATGGCA ACTCCGCTCT GCTTAGCCTG 
CCTGGCTACC GGCCTACCAC ACGAAGCTCT GCTCGTCGCT CCCAGGCCAG GATGTCCAGT 
GGGGCCCCCC AAGGGAGGAA TAGCTTCTAC ATGGGCACTT GCCAGGATGA GCCCGAGCAG 
CTGGATGATT GGAACCGCAT CGCAGAGTTG CAGCAGCGCA ACCGAGTATG CCCCCCGCAC 
CTGAAGACCT GCTACCCTCT GGAGTCCAGG CCTACCCTGA GCCTGGCCAC CATCACTGAT 
GAGGAGATGA AGACTGGAGA CCCCAGGGAA ACCTTGCGTA GAGCCAGCAT GCAGCCAGCT 
CAGATTGCTG AGGGTGTTGG CATAACTACC AGGCAGCAGC GCAAACGAGT TTCCTCTGAG 
ACCCACCAGG GCCCTGGCAC TCCTGAGTCC AAGAAAGCTA CCAGCTGCTT TCCACGCCCC 
ATGACCCCCA GGGACCGGCA TGAAGGACGT AAGCAGAGCA GCACTGCTGA CACCCAGAAG 
AAAGCAGCTC CAGTTCTCAA ACAGGCGGAC CGGCGTCAGT CAATGGCTTT CAGCATCCTT 
AACACACCTA AGAAGCTGGG AAATAGTCTT CTAAGGCGAG GAGCTTCAAA GAAGACCCCA 
GCAAAGGTCT CCCCCAATCC TCGCAGTGGA ACCCGCCGTT CTCCACGCAT TGCCACCACC 
ACAACTGGCA CTGCTACTGT TGCCACCACT CCTCGGGCCA AGGGCAAGGT GAAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Numa1
Entrez Gene ID	101706
Full Name	nuclear mitotic apparatus protein 1
Synonyms	6720401E04Rik,AA764025,AL022610,AU014979,NuMA
General protein information	Preferred Names nuclear mitotic apparatus protein 1 Names nuclear mitotic apparatus protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 E2

Gallus gallus (chicken)	NUMA1	NP_001177854.1
Pan troglodytes (chimpanzee)	NUMA1	XP_001174474.1
	NUMA1	XP_001114111.2
Rattus norvegicus (Norway rat)	Numa1	NP_001094161.1
Homo sapiens (human)	NUMA1	NP_006176.2
Canis lupus familiaris (dog)	NUMA1	XP_005633612.1
Bos taurus (cattle)	NUMA1	NP_001192675.1
Xenopus tropicalis (tropical clawed frog)	LOC100145664	XP_004920191.1
Mus musculus (house mouse)	Numa1	NP_598708.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Numa1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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