NUMA1 cDNA ORF clone, Bos taurus(cattle)

The following NUMA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUMA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa239163 NM_001205746.3
Latest version!
Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa216229 NM_001205746.2
Latest version!
Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa40571 NM_001205746.1
Latest version!
Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa26522 XM_005216171.3
Latest version!
Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa26522 XM_005216172.1
Latest version!
Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa26522 XM_010812655.2
Latest version!
Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa26522 XM_015474763.1
Latest version!
Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa26522 XM_005216174.3
Latest version!
Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa26522 XM_015474764.1
Latest version!
Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa26522 XM_005216173.3
Latest version!
Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa26523 XM_005216176.3
Latest version!
Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa239163
    Clone ID Related Accession (Same CDS sequence) NM_001205746.3
    Accession Version NM_001205746.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-11
    Organism Bos taurus(cattle)
    Product nuclear mitotic apparatus protein 1 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000015.1 and BF775779.1. On Jun 21, 2018 this sequence version replaced NM_001205746.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN05861916 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAAGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAACCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCCTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCAC
    GCTTGTGGAT TGCGAACCCA GTCCGAGGCT ACTGAAAAGA CTGAGCCAGA AGGCTCTGAA
    CTGCAGGCCC TGCAGGCAGA GGTGAGCCGG CTGGAACAGC AGGCCCGTGA GCACCAGGAG
    AAGGCCTCCA GCCTGGAGCA AAGCCTTGAG TCTGAGCGCG CTGCCCACGC GGAGCAGGCC
    AGTACTCTGA AGACTCTGCA GGGCCAGTTA GAGCAGAAGG CCCAGGAGCT GGGGAGCAGT
    CAGGACGCCT TAGCCTCAAC CCAGAGGGAG TTGGCCACCC TCCGTGCCAA GGCCCAGGAG
    CACAGCAAGG CCGAGGACGA GTGGAAGGCC CAGGTGGCCC GGGGTCAGCA GGAGGCCGAG
    AGGAAAAACA GCCTCATCAG CAGCTTGGAG GAGGAGGTGT CCATCCTGAA CCGCCAGGTG
    CTGGAGAAGG AGGGCGAGAG CAAGGAGCTG AAGCGGCTGG TGGTCGCTGA GTCAGAGAAG
    AGTCAGAAGC TGGAAGAGCG GCTGCGCCTG CTCCAGGCGG AGACCGCCAG CAGCAGCGCC
    AGGGCTGCGG AACGCAGCTC CGCTCTGCGT GAGGAGGTCC AGACCCTCCG GGAGGAGGCT
    GAGAAGCAGC GGGTGGCTTC TGAGAGCCTG CGGCAGGAGC TGGCATCGCA GGCGGAGCGA
    GCAGAGGAGC TGGGCCAAGA GTTAAAGGCC TGGCAGGAGA AGTTCTTTCA GAAGGAGCAA
    GCCCTCTCGG CCCTGCAGCT GGAGCACACC AGCACACAGG CCCTGGCGAG CGAGCTGCTG
    CCCGTCAAGC ACCTGTGCCA GCAGCTGCAG GCCGAGCAGG CTGCTGCCGA GAAGCGCCAC
    CGGGAGGAGC TGGAGCAGAG CAAGCAGGCG GCTGGCGGGC TGCGAGCAGA GCTGCTGCGG
    GCCCAGCGCG AGCTCGGGGA GTTGCTGCCT CTGCGGCAGA AGGTGGCAGA GCAGGAGCGC
    ATGGCCCAGC AGCTGCGAGC GGAGAAGGCC AGCTACGCGG AGCAACTGAG CATGCTGAAG
    AAGGCTCACG GCCTCTTGGC GGAGGAGAAC CGCGGGCTGG GCGAGCGGGC CAGCCTCGGC
    CGGCAGTTTC TGGAAGTGGA GCTGGACCAG GCCCGCGAGA AGTACAGCCA GGAGCTGGCA
    GCTGTGCGCG CTGACGCTGA GACCCGTCTG GCTGAGATGC AGCGGGAAGT GCAGAGCACT
    GCCCGGGAGC TGGAGGTGAT GACCGCCAAG TACGAGGGTG CCAAGGCCAA GGTCCTGGAG
    GAGAGGCAGC GTTTCCAGGA GGAGAGGCAG AAACTCACTG CCCAGGTGGA GCAGCTAGAG
    TTATTTCAGA GAGAGCAGAC TAAACAGGTG GAAGAACTGA GCAAGAAGCT AGCTGACCAT
    GAGCAAGCCA GCAAGGCGCA GCAGCAGAAG CTGAAGCGGT CATCAGCTTC TCTCCTTGTT
    CAGGCCCAGG GCGGTGAGAG CCAGCAGGAG GTCCAGCGCC TCCAGGCCCA GCTGAGTGAG
    CTGCAGGCCC AGCTGAGCCA GAAGGAGCAG GCGGCTGAGC ACTACAAGCT ACAGATGGAG
    AAGGCCAAGA CTCATTATGA TGCCAAGAAG CAGCAGAACC AAGAGCTGCA GGAGCAGCTG
    CGAGGCCTGG AGCAGCTGCA GACAGAGAAC AAGGAGCTGA GGGCAGAAGT GGATCGGCTG
    GGCCGGGAGC TGCAGCAGGC TGGGCTGAAG ACCAAGGAAG CCGAGCAGAC CTGCCGCCAC
    CTCACGGCCC AGGTGCGCAG CCTGGAGGCA CAGGTTGCCC ATGCCGACCA GCAGCTTCGG
    GACCTGGGCA AGTTCCAGGT GGCGACTGAT GCCTTGAAGA GCCGGGAGCC CCAGGCTAAG
    TCTCAGCTGG ACTTGAGTAT TGACAGCCTG GATCTGAGCT GCGAGGAGGG GACCCCTCTC
    TCTGTCACCA GCAAGTTGCC TCGCACCCAG CCAGACGGCA CCAGCATCCC CGGAGAGCCA
    GCCTCCCCCA TCTCCCAGCG TCTGCCCCCC AAAGTGGAGT CCCTGGAGAG CCTCTACTTC
    ACGCCCATCC CTGCTCGGGG TCAGGCCCCC CTGGAGAGCA GCCTGGACTC CCTGGGGGAC
    ATCTCCCTGG ACTCAAGCCG GAAAACCCGC TCCGCTCGTA GGCGCACCAC GCAGATCATC
    AACATCACCA TGACCAAGAA GCTGGACGTG GAGGATCCAG ACAGTGCCAA CGCCTCCTTC
    TACAGCACAC AGTCAGCCCC CACTTCCCAG GCAGGCCTGA GGGCCGCCTC CTCCACCCAG
    TCTCTAGCCC GCCTGGGCTC TCCCGACGAT GGCAACTCCA CTCTATTCAG CCTGCCTGGC
    TACCGGCCCA CCACTCGCAG CTCCGCTCGC CGTTCCCAGG CCGGGATGTC CAGTGGGGCC
    CCTCCAGGCA GGAACAGCTT CTATGTGGGC ACCTGCCAGG ACGAGCCCGA ACAGCTGGAT
    GACTGGAACC GCATTGCAGA GCTGCAGCAG CGCAACCGAG TATGCCCACC GCACCTGAAG
    ACCTGCTACC CCCTTGAGTC CAGGCCCTCC CTGAGCCTGC CTGCCATCAC AGATGAGGAG
    ATAAAAACCG GCGACCCCCG GGAGACCCTG CGCCGAGCCA GCATGCAGCC AACCCAGATA
    GCTGAGGGCG CTGGCATTAC CACACGGCAG CAGCGCAAGC GGGTTTCCTC AGAGACCCAC
    CAGGGCCCCG GCACACCCGA GTCAAAGAAG GCCACCACAT GTTTCCCGCG CCCCATGACC
    CCCCGGGACC GACACGAAGG GCGCAGACAC AGCAGTACCG AGGCCCAGAA GAAGGCAGTT
    CCAGCTGTTA AACAGGCTGA CCGCCGCCAG TCTATGGCCT TCAGCATCCT CAACACACCC
    AAGAAGCTTG GAAACAGCCT TCTGCGGAAG GCAGCCTCCA AGAAAGCCCC CTCCAAAGCC
    TCACCCAACC CCCGCAGTGG GACCCGCCGC TCTCCGCGCA TCGCCACCAC TGCAGCCAGC
    GCTGCCACTG CTGCTGCCAG CGCCGCTGCC ACCACCCCCC GGGCAAAGGG CAAGGCGAAG
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192675.3
    CDS169..6534
    Translation

    Target ORF information:

    RefSeq Version NM_001205746.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205746.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAAGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAACCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCCTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCAC
    GCTTGTGGAT TGCGAACCCA GTCCGAGGCT ACTGAAAAGA CTGAGCCAGA AGGCTCTGAA
    CTGCAGGCCC TGCAGGCAGA GGTGAGCCGG CTGGAACAGC AGGCCCGTGA GCACCAGGAG
    AAGGCCTCCA GCCTGGAGCA AAGCCTTGAG TCTGAGCGCG CTGCCCACGC GGAGCAGGCC
    AGTACTCTGA AGACTCTGCA GGGCCAGTTA GAGCAGAAGG CCCAGGAGCT GGGGAGCAGT
    CAGGACGCCT TAGCCTCAAC CCAGAGGGAG TTGGCCACCC TCCGTGCCAA GGCCCAGGAG
    CACAGCAAGG CCGAGGACGA GTGGAAGGCC CAGGTGGCCC GGGGTCAGCA GGAGGCCGAG
    AGGAAAAACA GCCTCATCAG CAGCTTGGAG GAGGAGGTGT CCATCCTGAA CCGCCAGGTG
    CTGGAGAAGG AGGGCGAGAG CAAGGAGCTG AAGCGGCTGG TGGTCGCTGA GTCAGAGAAG
    AGTCAGAAGC TGGAAGAGCG GCTGCGCCTG CTCCAGGCGG AGACCGCCAG CAGCAGCGCC
    AGGGCTGCGG AACGCAGCTC CGCTCTGCGT GAGGAGGTCC AGACCCTCCG GGAGGAGGCT
    GAGAAGCAGC GGGTGGCTTC TGAGAGCCTG CGGCAGGAGC TGGCATCGCA GGCGGAGCGA
    GCAGAGGAGC TGGGCCAAGA GTTAAAGGCC TGGCAGGAGA AGTTCTTTCA GAAGGAGCAA
    GCCCTCTCGG CCCTGCAGCT GGAGCACACC AGCACACAGG CCCTGGCGAG CGAGCTGCTG
    CCCGTCAAGC ACCTGTGCCA GCAGCTGCAG GCCGAGCAGG CTGCTGCCGA GAAGCGCCAC
    CGGGAGGAGC TGGAGCAGAG CAAGCAGGCG GCTGGCGGGC TGCGAGCAGA GCTGCTGCGG
    GCCCAGCGCG AGCTCGGGGA GTTGCTGCCT CTGCGGCAGA AGGTGGCAGA GCAGGAGCGC
    ATGGCCCAGC AGCTGCGAGC GGAGAAGGCC AGCTACGCGG AGCAACTGAG CATGCTGAAG
    AAGGCTCACG GCCTCTTGGC GGAGGAGAAC CGCGGGCTGG GCGAGCGGGC CAGCCTCGGC
    CGGCAGTTTC TGGAAGTGGA GCTGGACCAG GCCCGCGAGA AGTACAGCCA GGAGCTGGCA
    GCTGTGCGCG CTGACGCTGA GACCCGTCTG GCTGAGATGC AGCGGGAAGT GCAGAGCACT
    GCCCGGGAGC TGGAGGTGAT GACCGCCAAG TACGAGGGTG CCAAGGCCAA GGTCCTGGAG
    GAGAGGCAGC GTTTCCAGGA GGAGAGGCAG AAACTCACTG CCCAGGTGGA GCAGCTAGAG
    TTATTTCAGA GAGAGCAGAC TAAACAGGTG GAAGAACTGA GCAAGAAGCT AGCTGACCAT
    GAGCAAGCCA GCAAGGCGCA GCAGCAGAAG CTGAAGCGGT CATCAGCTTC TCTCCTTGTT
    CAGGCCCAGG GCGGTGAGAG CCAGCAGGAG GTCCAGCGCC TCCAGGCCCA GCTGAGTGAG
    CTGCAGGCCC AGCTGAGCCA GAAGGAGCAG GCGGCTGAGC ACTACAAGCT ACAGATGGAG
    AAGGCCAAGA CTCATTATGA TGCCAAGAAG CAGCAGAACC AAGAGCTGCA GGAGCAGCTG
    CGAGGCCTGG AGCAGCTGCA GACAGAGAAC AAGGAGCTGA GGGCAGAAGT GGATCGGCTG
    GGCCGGGAGC TGCAGCAGGC TGGGCTGAAG ACCAAGGAAG CCGAGCAGAC CTGCCGCCAC
    CTCACGGCCC AGGTGCGCAG CCTGGAGGCA CAGGTTGCCC ATGCCGACCA GCAGCTTCGG
    GACCTGGGCA AGTTCCAGGT GGCGACTGAT GCCTTGAAGA GCCGGGAGCC CCAGGCTAAG
    TCTCAGCTGG ACTTGAGTAT TGACAGCCTG GATCTGAGCT GCGAGGAGGG GACCCCTCTC
    TCTGTCACCA GCAAGTTGCC TCGCACCCAG CCAGACGGCA CCAGCATCCC CGGAGAGCCA
    GCCTCCCCCA TCTCCCAGCG TCTGCCCCCC AAAGTGGAGT CCCTGGAGAG CCTCTACTTC
    ACGCCCATCC CTGCTCGGGG TCAGGCCCCC CTGGAGAGCA GCCTGGACTC CCTGGGGGAC
    ATCTCCCTGG ACTCAAGCCG GAAAACCCGC TCCGCTCGTA GGCGCACCAC GCAGATCATC
    AACATCACCA TGACCAAGAA GCTGGACGTG GAGGATCCAG ACAGTGCCAA CGCCTCCTTC
    TACAGCACAC AGTCAGCCCC CACTTCCCAG GCAGGCCTGA GGGCCGCCTC CTCCACCCAG
    TCTCTAGCCC GCCTGGGCTC TCCCGACGAT GGCAACTCCA CTCTATTCAG CCTGCCTGGC
    TACCGGCCCA CCACTCGCAG CTCCGCTCGC CGTTCCCAGG CCGGGATGTC CAGTGGGGCC
    CCTCCAGGCA GGAACAGCTT CTATGTGGGC ACCTGCCAGG ACGAGCCCGA ACAGCTGGAT
    GACTGGAACC GCATTGCAGA GCTGCAGCAG CGCAACCGAG TATGCCCACC GCACCTGAAG
    ACCTGCTACC CCCTTGAGTC CAGGCCCTCC CTGAGCCTGC CTGCCATCAC AGATGAGGAG
    ATAAAAACCG GCGACCCCCG GGAGACCCTG CGCCGAGCCA GCATGCAGCC AACCCAGATA
    GCTGAGGGCG CTGGCATTAC CACACGGCAG CAGCGCAAGC GGGTTTCCTC AGAGACCCAC
    CAGGGCCCCG GCACACCCGA GTCAAAGAAG GCCACCACAT GTTTCCCGCG CCCCATGACC
    CCCCGGGACC GACACGAAGG GCGCAGACAC AGCAGTACCG AGGCCCAGAA GAAGGCAGTT
    CCAGCTGTTA AACAGGCTGA CCGCCGCCAG TCTATGGCCT TCAGCATCCT CAACACACCC
    AAGAAGCTTG GAAACAGCCT TCTGCGGAAG GCAGCCTCCA AGAAAGCCCC CTCCAAAGCC
    TCACCCAACC CCCGCAGTGG GACCCGCCGC TCTCCGCGCA TCGCCACCAC TGCAGCCAGC
    GCTGCCACTG CTGCTGCCAG CGCCGCTGCC ACCACCCCCC GGGCAAAGGG CAAGGCGAAG
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216229
    Clone ID Related Accession (Same CDS sequence) NM_001205746.2
    Accession Version NM_001205746.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product nuclear mitotic apparatus protein 1 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02040998.1. On Jun 30, 2017 this sequence version replaced NM_001205746.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN04019650 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCAC
    GCTTGTGGAT TGCGAACCCA GTCCGAGGCT ACTGAAAAGA CTGAGCCAGA AGGCTCTGAA
    CTGCAGGCCC TGCAGGCAGA GGTGAGCCGG CTGGAACAGC AGGCCCGTGA GCACCAGGAG
    AAGGCCTCCA GCCTGGAGCA AAGCCTTGAG TCTGAGCGCG CTGCCCACGC GGAGCAGGCC
    AGTACTCTGA AGACTCTGCA GGGCCAGTTA GAGCAGAAGG CCCAGGAGCT GGGGAGCAGT
    CAGGACGCCT TAGCCTCAAC CCAGAGGGAG TTGGCCACCC TCCGTGCCAA GGCCCAGGAG
    CACAGCAAGG CCGAGGACGA GTGGAAGGCC CAGGTGGCCC GGGGTCAGCA GGAGGCCGAG
    AGGAAAAACA GCCTCATCAG CAGCTTGGAG GAGGAGGTGT CCATCCTGAA CCGCCAGGTG
    CTGGAGAAGG AGGGCGAGAG CAAGGAGCTG AAGCGGCTGG TGGTCGCTGA GTCAGAGAAG
    AGTCAGAAGC TGGAAGAGCG GCTGCGCCTG CTCCAGGCGG AGACCGCCAG CAGCAGCGCC
    AGGGCTGCGG AACGCAGCTC CGCTCTGCGT GAGGAGGTCC AGACCCTCCG GGAGGAGGCT
    GAGAAGCAGC GGGTGGCTTC TGAGAGCCTG CGGCAGGAGC TGGCATCGCA GGCGGAGCGA
    GCAGAGGAGC TGGGCCAAGA GTTAAAGGCC TGGCAGGAGA AGTTCTTTCA GAAGGAGCAA
    GCCCTCTCGG CCCTGCAGCT GGAGCACACC AGCACACAGG CCCTGGCGAG CGAGCTGCTG
    CCCGTCAAGC ACCTGTGCCA GCAGCTGCAG GCCGAGCAGG CTGCTGCCGA GAAGCGCCAC
    CGGGAGGAGC TGGAGCAGAG CAAGCAGGCG GCTGGCGGGC TGCGAGCAGA GCTGCTGCGG
    GCCCAGCGCG AGCTCGGGGA GTTGCTGCCT CTGCGGCAGA AGGTGGCAGA GCAGGAGCGC
    ATGGCCCAGC AGCTGCGAGC GGAGAAGGCC AGCTACGCGG AGCAACTGAG CATGCTGAAG
    AAGGCTCACG GCCTCTTGGC GGAGGAGAAC CGCGGGCTGG GCGAGCGGGC CAGCCTCGGC
    CGGCAGTTTC TGGAAGTGGA GCTGGACCAG GCCCGCGAGA AGTACAGCCA GGAGCTGGCA
    GCTGTGCGCG CTGACGCTGA GACCCGTCTG GCTGAGATGC AGCGGGAAGT GCAGAGCACT
    GCCCGGGAGC TGGAGGTGAT GACCGCCAAG TACGAGGGTG CCAAGGCCAA GGTCCTGGAG
    GAGAGGCAGC GTTTCCAGGA GGAGAGGCAG AAACTCACTG CCCAGGTGGA GCAGCTAGAG
    TTATTTCAGA GAGAGCAGAC TAAACAGGTG GAAGAACTGA GCAAGAAGCT AGCTGACCAT
    GAGCAAGCCA GCAAGGCGCA GCAGCAGAAG CTGAAGCGGT CATCAGCTTC TCTCCTTGTT
    CAGGCCCAGG GCGGTGAGAG CCAGCAGGAG GTCCAGCGCC TCCAGGCCCA GCTGAGTGAG
    CTGCAGGCCC AGCTGAGCCA GAAGGAGCAG GCGGCTGAGC ACTACAAGCT ACAGATGGAG
    AAGGCCAAGA CTCATTATGA TGCCAAGAAG CAGCAGAACC AAGAGCTGCA GGAGCAGCTG
    CGAGGCCTGG AGCAGCTGCA GACAGAGAAC AAGGAGCTGA GGGCAGAAGT GGATCGGCTG
    GGCCGGGAGC TGCAGCAGGC TGGGCTGAAG ACCAAGGAAG CCGAGCAGAC CTGCCGCCAC
    CTCACGGCCC AGGTGCGCAG CCTGGAGGCA CAGGTTGCCC ATGCCGACCA GCAGCTTCGG
    GACCTGGGCA AGTTCCAGGT GGCGACTGAT GCCTTGAAGA GCCGGGAGCC CCAGGCTAAG
    TCTCAGCTGG ACTTGAGTAT TGACAGCCTG GATCTGAGCT GCGAGGAGGG GACCCCTCTC
    TCTGTCACCA GCAAGTTGCC TCGCACCCAG CCAGACGGCA CCAGCATCCC CGGAGAGCCA
    GCCTCCCCCA TCTCCCAGCG TCTGCCCCCC AAAGTGGAGT CCCTGGAGAG CCTCTACTTC
    ACGCCCATCC CTGCTCGGGG TCAGGCCCCC CTGGAGAGCA GCCTGGACTC CCTGGGGGAC
    ATCTCCCTGG ACTCAAGCCG GAAAACCCGC TCCGCTCGTA GGCGCACCAC GCAGATCATC
    AACATCACCA TGACCAAGAA GCTGGACGTG GAGGATCCAG ACAGTGCCAA CGCCTCCTTC
    TACAGCACAC AGTCAGCCCC CACTTCCCAG GCAGGCCTGA GGGCCGCCTC CTCCACCCAG
    TCTCTAGCCC GCCTGGGCTC TCCCGACGAT GGCAACTCCA CTCTATTCAG CCTGCCTGGC
    TACCGGCCCA CCACTCGCAG CTCCGCTCGC CGTTCCCAGG CCGGGATGTC CAGTGGGGCC
    CCTCCAGGCA GGAACAGCTT CTATGTGGGC ACCTGCCAGG ACGAGCCCGA ACAGCTGGAT
    GACTGGAACC GCATTGCAGA GCTGCAGCAG CGCAACCGAG TATGCCCACC GCACCTGAAG
    ACCTGCTACC CCCTTGAGTC CAGGCCCTCC CTGAGCCTGC CTGCCATCAC AGATGAGGAG
    ATAAAAACCG GCGACCCCCG GGAGACCCTG CGCCGAGCCA GCATGCAGCC AACCCAGATA
    GCTGAGGGCG CTGGCATTAC CACACGGCAG CAGCGCAAGC GGGTTTCCTC AGAGACCCAC
    CAGGGCCCCG GCACACCCGA GTCAAAGAAG GCCACCACAT GTTTCCCGCG CCCCATGACC
    CCCCGGGACC GACACGAAGG GCGCAGACAC AGCAGTACCG AGGCCCAGAA GAAGGCAGTT
    CCAGCTGTTA AACAGGCTGA CCGCCGCCAG TCTATGGCCT TCAGCATCCT CAACACACCC
    AAGAAGCTTG GAAACAGCCT TCTGCGGAAG GCAGCCTCCA AGAAAGCCCC CTCCAAAGCC
    TCACCCAACC CCCGCAGTGG GACCCGCCGC TCTCCGCGCA TCGCCACCAC TGCAGCCAGC
    GCTGCCACTG CTGCTGCCAG CGCCGCTGCC ACCACCCCCC GGGCAAAGGG CAAGGCGAAG
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192675.2
    CDS169..6534
    Translation

    Target ORF information:

    RefSeq Version NM_001205746.2
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205746.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCAC
    GCTTGTGGAT TGCGAACCCA GTCCGAGGCT ACTGAAAAGA CTGAGCCAGA AGGCTCTGAA
    CTGCAGGCCC TGCAGGCAGA GGTGAGCCGG CTGGAACAGC AGGCCCGTGA GCACCAGGAG
    AAGGCCTCCA GCCTGGAGCA AAGCCTTGAG TCTGAGCGCG CTGCCCACGC GGAGCAGGCC
    AGTACTCTGA AGACTCTGCA GGGCCAGTTA GAGCAGAAGG CCCAGGAGCT GGGGAGCAGT
    CAGGACGCCT TAGCCTCAAC CCAGAGGGAG TTGGCCACCC TCCGTGCCAA GGCCCAGGAG
    CACAGCAAGG CCGAGGACGA GTGGAAGGCC CAGGTGGCCC GGGGTCAGCA GGAGGCCGAG
    AGGAAAAACA GCCTCATCAG CAGCTTGGAG GAGGAGGTGT CCATCCTGAA CCGCCAGGTG
    CTGGAGAAGG AGGGCGAGAG CAAGGAGCTG AAGCGGCTGG TGGTCGCTGA GTCAGAGAAG
    AGTCAGAAGC TGGAAGAGCG GCTGCGCCTG CTCCAGGCGG AGACCGCCAG CAGCAGCGCC
    AGGGCTGCGG AACGCAGCTC CGCTCTGCGT GAGGAGGTCC AGACCCTCCG GGAGGAGGCT
    GAGAAGCAGC GGGTGGCTTC TGAGAGCCTG CGGCAGGAGC TGGCATCGCA GGCGGAGCGA
    GCAGAGGAGC TGGGCCAAGA GTTAAAGGCC TGGCAGGAGA AGTTCTTTCA GAAGGAGCAA
    GCCCTCTCGG CCCTGCAGCT GGAGCACACC AGCACACAGG CCCTGGCGAG CGAGCTGCTG
    CCCGTCAAGC ACCTGTGCCA GCAGCTGCAG GCCGAGCAGG CTGCTGCCGA GAAGCGCCAC
    CGGGAGGAGC TGGAGCAGAG CAAGCAGGCG GCTGGCGGGC TGCGAGCAGA GCTGCTGCGG
    GCCCAGCGCG AGCTCGGGGA GTTGCTGCCT CTGCGGCAGA AGGTGGCAGA GCAGGAGCGC
    ATGGCCCAGC AGCTGCGAGC GGAGAAGGCC AGCTACGCGG AGCAACTGAG CATGCTGAAG
    AAGGCTCACG GCCTCTTGGC GGAGGAGAAC CGCGGGCTGG GCGAGCGGGC CAGCCTCGGC
    CGGCAGTTTC TGGAAGTGGA GCTGGACCAG GCCCGCGAGA AGTACAGCCA GGAGCTGGCA
    GCTGTGCGCG CTGACGCTGA GACCCGTCTG GCTGAGATGC AGCGGGAAGT GCAGAGCACT
    GCCCGGGAGC TGGAGGTGAT GACCGCCAAG TACGAGGGTG CCAAGGCCAA GGTCCTGGAG
    GAGAGGCAGC GTTTCCAGGA GGAGAGGCAG AAACTCACTG CCCAGGTGGA GCAGCTAGAG
    TTATTTCAGA GAGAGCAGAC TAAACAGGTG GAAGAACTGA GCAAGAAGCT AGCTGACCAT
    GAGCAAGCCA GCAAGGCGCA GCAGCAGAAG CTGAAGCGGT CATCAGCTTC TCTCCTTGTT
    CAGGCCCAGG GCGGTGAGAG CCAGCAGGAG GTCCAGCGCC TCCAGGCCCA GCTGAGTGAG
    CTGCAGGCCC AGCTGAGCCA GAAGGAGCAG GCGGCTGAGC ACTACAAGCT ACAGATGGAG
    AAGGCCAAGA CTCATTATGA TGCCAAGAAG CAGCAGAACC AAGAGCTGCA GGAGCAGCTG
    CGAGGCCTGG AGCAGCTGCA GACAGAGAAC AAGGAGCTGA GGGCAGAAGT GGATCGGCTG
    GGCCGGGAGC TGCAGCAGGC TGGGCTGAAG ACCAAGGAAG CCGAGCAGAC CTGCCGCCAC
    CTCACGGCCC AGGTGCGCAG CCTGGAGGCA CAGGTTGCCC ATGCCGACCA GCAGCTTCGG
    GACCTGGGCA AGTTCCAGGT GGCGACTGAT GCCTTGAAGA GCCGGGAGCC CCAGGCTAAG
    TCTCAGCTGG ACTTGAGTAT TGACAGCCTG GATCTGAGCT GCGAGGAGGG GACCCCTCTC
    TCTGTCACCA GCAAGTTGCC TCGCACCCAG CCAGACGGCA CCAGCATCCC CGGAGAGCCA
    GCCTCCCCCA TCTCCCAGCG TCTGCCCCCC AAAGTGGAGT CCCTGGAGAG CCTCTACTTC
    ACGCCCATCC CTGCTCGGGG TCAGGCCCCC CTGGAGAGCA GCCTGGACTC CCTGGGGGAC
    ATCTCCCTGG ACTCAAGCCG GAAAACCCGC TCCGCTCGTA GGCGCACCAC GCAGATCATC
    AACATCACCA TGACCAAGAA GCTGGACGTG GAGGATCCAG ACAGTGCCAA CGCCTCCTTC
    TACAGCACAC AGTCAGCCCC CACTTCCCAG GCAGGCCTGA GGGCCGCCTC CTCCACCCAG
    TCTCTAGCCC GCCTGGGCTC TCCCGACGAT GGCAACTCCA CTCTATTCAG CCTGCCTGGC
    TACCGGCCCA CCACTCGCAG CTCCGCTCGC CGTTCCCAGG CCGGGATGTC CAGTGGGGCC
    CCTCCAGGCA GGAACAGCTT CTATGTGGGC ACCTGCCAGG ACGAGCCCGA ACAGCTGGAT
    GACTGGAACC GCATTGCAGA GCTGCAGCAG CGCAACCGAG TATGCCCACC GCACCTGAAG
    ACCTGCTACC CCCTTGAGTC CAGGCCCTCC CTGAGCCTGC CTGCCATCAC AGATGAGGAG
    ATAAAAACCG GCGACCCCCG GGAGACCCTG CGCCGAGCCA GCATGCAGCC AACCCAGATA
    GCTGAGGGCG CTGGCATTAC CACACGGCAG CAGCGCAAGC GGGTTTCCTC AGAGACCCAC
    CAGGGCCCCG GCACACCCGA GTCAAAGAAG GCCACCACAT GTTTCCCGCG CCCCATGACC
    CCCCGGGACC GACACGAAGG GCGCAGACAC AGCAGTACCG AGGCCCAGAA GAAGGCAGTT
    CCAGCTGTTA AACAGGCTGA CCGCCGCCAG TCTATGGCCT TCAGCATCCT CAACACACCC
    AAGAAGCTTG GAAACAGCCT TCTGCGGAAG GCAGCCTCCA AGAAAGCCCC CTCCAAAGCC
    TCACCCAACC CCCGCAGTGG GACCCGCCGC TCTCCGCGCA TCGCCACCAC TGCAGCCAGC
    GCTGCCACTG CTGCTGCCAG CGCCGCTGCC ACCACCCCCC GGGCAAAGGG CAAGGCGAAG
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40571
    Clone ID Related Accession (Same CDS sequence) NM_001205746.1
    Accession Version NM_001205746.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product nuclear mitotic apparatus protein 1 precursor
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03024973.1 and AAFC03077677.1. On Apr 20, 2011 this sequence version replaced XM_590721.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA AAGAAAAACA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCAC
    GCTTGTGGAT TGCGAACCCA GTCCGAGGCT ACTGAAAAGA CTGAGCCAGA AGGCTCTGAA
    CTGCAGGCCC TGCAGGCAGA GGTGAGCCGG CTGGAACAGC AGGCCCGTGA GCACCAGGAG
    AAGGCCTCCA GCCTGGAGCA AAGCCTTGAG TCTGAGCGCG CTGCCCACGC GGAGCAGGCC
    AGTACTCTGA AGACTCTGCA GGGCCAGTTA GAGCAGAAGG CCCAGGAGCT GGGGAGCAGT
    CAGGACGCCT TAGCCTCAAC CCAGAGGGAG TTGGCCACCC TCCGTGCCAA GGCCCAGGAG
    CACAGCAAGG CCGAGGACGA GTGGAAGGCC CAGGTGGCCC GGGGTCAGCA GGAGGCCGAG
    AGGAAAAACA GCCTCATCAG CAGCTTGGAG GAGGAGGTGT CCATCCTGAA CCGCCAGGTG
    CTGGAGAAGG AGGGCGAGAG CAAGGAGCTG AAGCGGCTGG TGGTCGCTGA GTCAGAGAAG
    AGTCAGAAGC TGGAAGAGCG GCTGCGCCTG CTCCAGGCGG AGACCGCCAG CAGCAGCGCC
    AGGGCTGCGG AACGCAGCTC CGCTCTGCGT GAGGAGGTCC AGACCCTCCG GGAGGAGGCT
    GAGAAGCAGC GGGTGGCTTC TGAGAGCCTG CGGCAGGAGC TGGCATCGCA GGCGGAGCGA
    GCAGAGGAGC TGGGCCAAGA GTTAAAGGCC TGGCAGGAGA AGTTCTTTCA GAAGGAGCAA
    GCCCTCTCGG CCCTGCAGCT GGAGCACACC AGCACACAGG CCCTGGCGAG CGAGCTGCTG
    CCCGTCAAGC ACCTGTGCCA GCAGCTGCAG GCCGAGCAGG CTGCTGCCGA GAAGCGCCAC
    CGGGAGGAGC TGGAGCAGAG CAAGCAGGCG GCTGGCGGGC TGCGAGCAGA GCTGCTGCGG
    GCCCAGCGCG AGCTCGGGGA GTTGCTGCCT CTGCGGCAGA AGGTGGCAGA GCAGGAGCGC
    ATGGCCCAGC AGCTGCGAGC GGAGAAGGCC AGCTACGCGG AGCAACTGAG CATGCTGAAG
    AAGGCTCACG GCCTCTTGGC GGAGGAGAAC CGCGGGCTGG GCGAGCGGGC CAGCCTCGGC
    CGGCAGTTTC TGGAAGTGGA GCTGGACCAG GCCCGCGAGA AGTACAGCCA GGAGCTGGCA
    GCTGTGCGCG CTGACGCTGA GACCCGTCTG GCTGAGATGC AGCGGGAAGT GCAGAGCACT
    GCCCGGGAGC TGGAGGTGAT GACCGCCAAG TACGAGGGTG CCAAGGCCAA GGTCCTGGAG
    GAGAGGCAGC GTTTCCAGGA GGAGAGGCAG AAACTCACTG CCCAGGTGGA GCAGCTAGAG
    TTATTTCAGA GAGAGCAGAC TAAACAGGTG GAAGAACTGA GCAAGAAGCT AGCTGACCAT
    GAGCAAGCCA GCAAGGCGCA GCAGCAGAAG CTGAAGCGGT CATCAGCTTC TCTCCTTGTT
    CAGGCCCAGG GCGGTGAGAG CCAGCAGGAG GTCCAGCGCC TCCAGGCCCA GCTGAGTGAG
    CTGCAGGCCC AGCTGAGCCA GAAGGAGCAG GCGGCTGAGC ACTACAAGCT ACAGATGGAG
    AAGGCCAAGA CTCATTATGA TGCCAAGAAG CAGCAGAACC AAGAGCTGCA GGAGCAGCTG
    CGAGGCCTGG AGCAGCTGCA GACAGAGAAC AAGGAGCTGA GGGCAGAAGT GGATCGGCTG
    GGCCGGGAGC TGCAGCAGGC TGGGCTGAAG ACCAAGGAAG CCGAGCAGAC CTGCCGCCAC
    CTCACGGCCC AGGTGCGCAG CCTGGAGGCA CAGGTTGCCC ATGCCGACCA GCAGCTTCGG
    GACCTGGGCA AGTTCCAGGT GGCGACTGAT GCCTTGAAGA GCCGGGAGCC CCAGGCTAAG
    TCTCAGCTGG ACTTGAGTAT TGACAGCCTG GATCTGAGCT GCGAGGAGGG GACCCCTCTC
    TCTGTCACCA GCAAGTTGCC TCGCACCCAG CCAGACGGCA CCAGCATCCC CGGAGAGCCA
    GCCTCCCCCA TCTCCCAGCG TCTGCCCCCC AAAGTGGAGT CCCTGGAGAG CCTCTACTTC
    ACGCCCATCC CTGCTCGGGG TCAGGCCCCC CTGGAGAGCA GCCTGGACTC CCTGGGGGAC
    ATCTCCCTGG ACTCAAGCCG GAAAACCCGC TCCGCTCGTA GGCGCACCAC GCAGATCATC
    AACATCACCA TGACCAAGAA GCTGGACGTG GAGGATCCAG ACAGTGCCAA CGCCTCCTTC
    TACAGCACAC AGTCAGCCCC CACTTCCCAG GCAGGCCTGA GGGCCGCCTC CTCCACCCAG
    TCTCTAGCCC GCCTGGGCTC TCCCGACGAT GGCAACTCCA CTCTATTCAG CCTGCCTGGC
    TACCGGCCCA CCACTCGCAG CTCCGCTCGC CGTTCCCAGG CCGGGATGTC CAGTGGGGCC
    CCTCCAGGCA GGAACAGCTT CTATGTGGGC ACCTGCCAGG ACGAGCCCGA ACAGCTGGAT
    GACTGGAACC GCATTGCAGA GCTGCAGCAG CGCAACCGAG TATGCCCACC GCACCTGAAG
    ACCTGCTACC CCCTTGAGTC CAGGCCCTCC CTGAGCCTGC CTGCCATCAC AGATGAGGAG
    ATAAAAACCG GCGACCCCCG GGAGACCCTG CGCCGAGCCA GCATGCAGCC AACCCAGATA
    GCTGAGGGCG CTGGCATTAC CACACGGCAG CAGCGCAAGC GGGTTTCCTC AGAGACCCAC
    CAGGGCCCCG GCACACCCGA GTCAAAGAAG GCCACCACAT GTTTCCCGCG CCCCATGACC
    CCCCGGGACC GACACGAAGG GCGCAGACAC AGCAGTACCG AGGCCCAGAA GAAGGCAGTT
    CCAGCTGTTA AACAGGCTGA CCGCCGCCAG TCTATGGCCT TCAGCATCCT CAACACACCC
    AAGAAGCTTG GAAACAGCCT TCTGCGGAAG GCAGCCTCCA AGAAAGCCCC CTCCAAAGCC
    TCACCCAACC CCCGCAGTGG GACCCGCCGC TCTCCGCGCA TCGCCACCAC TGCAGCCAGC
    GCTGCCACTG CTGCTGCCAG CGCCGCTGCC ACCACCCCCC GGGCAAAGGG CAAGGCGAAG
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192675.1
    CDS169..6534
    Translation

    Target ORF information:

    RefSeq Version NM_001205746.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205746.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA AAGAAAAACA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCAC
    GCTTGTGGAT TGCGAACCCA GTCCGAGGCT ACTGAAAAGA CTGAGCCAGA AGGCTCTGAA
    CTGCAGGCCC TGCAGGCAGA GGTGAGCCGG CTGGAACAGC AGGCCCGTGA GCACCAGGAG
    AAGGCCTCCA GCCTGGAGCA AAGCCTTGAG TCTGAGCGCG CTGCCCACGC GGAGCAGGCC
    AGTACTCTGA AGACTCTGCA GGGCCAGTTA GAGCAGAAGG CCCAGGAGCT GGGGAGCAGT
    CAGGACGCCT TAGCCTCAAC CCAGAGGGAG TTGGCCACCC TCCGTGCCAA GGCCCAGGAG
    CACAGCAAGG CCGAGGACGA GTGGAAGGCC CAGGTGGCCC GGGGTCAGCA GGAGGCCGAG
    AGGAAAAACA GCCTCATCAG CAGCTTGGAG GAGGAGGTGT CCATCCTGAA CCGCCAGGTG
    CTGGAGAAGG AGGGCGAGAG CAAGGAGCTG AAGCGGCTGG TGGTCGCTGA GTCAGAGAAG
    AGTCAGAAGC TGGAAGAGCG GCTGCGCCTG CTCCAGGCGG AGACCGCCAG CAGCAGCGCC
    AGGGCTGCGG AACGCAGCTC CGCTCTGCGT GAGGAGGTCC AGACCCTCCG GGAGGAGGCT
    GAGAAGCAGC GGGTGGCTTC TGAGAGCCTG CGGCAGGAGC TGGCATCGCA GGCGGAGCGA
    GCAGAGGAGC TGGGCCAAGA GTTAAAGGCC TGGCAGGAGA AGTTCTTTCA GAAGGAGCAA
    GCCCTCTCGG CCCTGCAGCT GGAGCACACC AGCACACAGG CCCTGGCGAG CGAGCTGCTG
    CCCGTCAAGC ACCTGTGCCA GCAGCTGCAG GCCGAGCAGG CTGCTGCCGA GAAGCGCCAC
    CGGGAGGAGC TGGAGCAGAG CAAGCAGGCG GCTGGCGGGC TGCGAGCAGA GCTGCTGCGG
    GCCCAGCGCG AGCTCGGGGA GTTGCTGCCT CTGCGGCAGA AGGTGGCAGA GCAGGAGCGC
    ATGGCCCAGC AGCTGCGAGC GGAGAAGGCC AGCTACGCGG AGCAACTGAG CATGCTGAAG
    AAGGCTCACG GCCTCTTGGC GGAGGAGAAC CGCGGGCTGG GCGAGCGGGC CAGCCTCGGC
    CGGCAGTTTC TGGAAGTGGA GCTGGACCAG GCCCGCGAGA AGTACAGCCA GGAGCTGGCA
    GCTGTGCGCG CTGACGCTGA GACCCGTCTG GCTGAGATGC AGCGGGAAGT GCAGAGCACT
    GCCCGGGAGC TGGAGGTGAT GACCGCCAAG TACGAGGGTG CCAAGGCCAA GGTCCTGGAG
    GAGAGGCAGC GTTTCCAGGA GGAGAGGCAG AAACTCACTG CCCAGGTGGA GCAGCTAGAG
    TTATTTCAGA GAGAGCAGAC TAAACAGGTG GAAGAACTGA GCAAGAAGCT AGCTGACCAT
    GAGCAAGCCA GCAAGGCGCA GCAGCAGAAG CTGAAGCGGT CATCAGCTTC TCTCCTTGTT
    CAGGCCCAGG GCGGTGAGAG CCAGCAGGAG GTCCAGCGCC TCCAGGCCCA GCTGAGTGAG
    CTGCAGGCCC AGCTGAGCCA GAAGGAGCAG GCGGCTGAGC ACTACAAGCT ACAGATGGAG
    AAGGCCAAGA CTCATTATGA TGCCAAGAAG CAGCAGAACC AAGAGCTGCA GGAGCAGCTG
    CGAGGCCTGG AGCAGCTGCA GACAGAGAAC AAGGAGCTGA GGGCAGAAGT GGATCGGCTG
    GGCCGGGAGC TGCAGCAGGC TGGGCTGAAG ACCAAGGAAG CCGAGCAGAC CTGCCGCCAC
    CTCACGGCCC AGGTGCGCAG CCTGGAGGCA CAGGTTGCCC ATGCCGACCA GCAGCTTCGG
    GACCTGGGCA AGTTCCAGGT GGCGACTGAT GCCTTGAAGA GCCGGGAGCC CCAGGCTAAG
    TCTCAGCTGG ACTTGAGTAT TGACAGCCTG GATCTGAGCT GCGAGGAGGG GACCCCTCTC
    TCTGTCACCA GCAAGTTGCC TCGCACCCAG CCAGACGGCA CCAGCATCCC CGGAGAGCCA
    GCCTCCCCCA TCTCCCAGCG TCTGCCCCCC AAAGTGGAGT CCCTGGAGAG CCTCTACTTC
    ACGCCCATCC CTGCTCGGGG TCAGGCCCCC CTGGAGAGCA GCCTGGACTC CCTGGGGGAC
    ATCTCCCTGG ACTCAAGCCG GAAAACCCGC TCCGCTCGTA GGCGCACCAC GCAGATCATC
    AACATCACCA TGACCAAGAA GCTGGACGTG GAGGATCCAG ACAGTGCCAA CGCCTCCTTC
    TACAGCACAC AGTCAGCCCC CACTTCCCAG GCAGGCCTGA GGGCCGCCTC CTCCACCCAG
    TCTCTAGCCC GCCTGGGCTC TCCCGACGAT GGCAACTCCA CTCTATTCAG CCTGCCTGGC
    TACCGGCCCA CCACTCGCAG CTCCGCTCGC CGTTCCCAGG CCGGGATGTC CAGTGGGGCC
    CCTCCAGGCA GGAACAGCTT CTATGTGGGC ACCTGCCAGG ACGAGCCCGA ACAGCTGGAT
    GACTGGAACC GCATTGCAGA GCTGCAGCAG CGCAACCGAG TATGCCCACC GCACCTGAAG
    ACCTGCTACC CCCTTGAGTC CAGGCCCTCC CTGAGCCTGC CTGCCATCAC AGATGAGGAG
    ATAAAAACCG GCGACCCCCG GGAGACCCTG CGCCGAGCCA GCATGCAGCC AACCCAGATA
    GCTGAGGGCG CTGGCATTAC CACACGGCAG CAGCGCAAGC GGGTTTCCTC AGAGACCCAC
    CAGGGCCCCG GCACACCCGA GTCAAAGAAG GCCACCACAT GTTTCCCGCG CCCCATGACC
    CCCCGGGACC GACACGAAGG GCGCAGACAC AGCAGTACCG AGGCCCAGAA GAAGGCAGTT
    CCAGCTGTTA AACAGGCTGA CCGCCGCCAG TCTATGGCCT TCAGCATCCT CAACACACCC
    AAGAAGCTTG GAAACAGCCT TCTGCGGAAG GCAGCCTCCA AGAAAGCCCC CTCCAAAGCC
    TCACCCAACC CCCGCAGTGG GACCCGCCGC TCTCCGCGCA TCGCCACCAC TGCAGCCAGC
    GCTGCCACTG CTGCTGCCAG CGCCGCTGCC ACCACCCCCC GGGCAAAGGG CAAGGCGAAG
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26522
    Clone ID Related Accession (Same CDS sequence) XM_005216174.3 , XM_010812655.2 , XM_005216172.1 , XM_015474764.1 , XM_005216171.3 , XM_015474763.1 , XM_005216173.3
    Accession Version XM_005216174.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear mitotic apparatus protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104415.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216174.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCGC
    CCGCAGTCTC TTGGGACAGC CAGCCGAGAA GACGCTTGTG GATTGCGAAC CCAGTCCGAG
    GCTACTGAAA AGACTGAGCC AGAAGGCTCT GAACTGCAGG CCCTGCAGGC AGAGGTGAGC
    CGGCTGGAAC AGCAGGCCCG TGAGCACCAG GAGAAGGCCT CCAGCCTGGA GCAAAGCCTT
    GAGTCTGAGC GCGCTGCCCA CGCGGAGCAG GCCAGTACTC TGAAGACTCT GCAGGGCCAG
    TTAGAGCAGA AGGCCCAGGA GCTGGGGAGC AGTCAGGACG CCTTAGCCTC AACCCAGAGG
    GAGTTGGCCA CCCTCCGTGC CAAGGCCCAG GAGCACAGCA AGGCCGAGGA CGAGTGGAAG
    GCCCAGGTGG CCCGGGGTCA GCAGGAGGCC GAGAGGAAAA ACAGCCTCAT CAGCAGCTTG
    GAGGAGGAGG TGTCCATCCT GAACCGCCAG GTGCTGGAGA AGGAGGGCGA GAGCAAGGAG
    CTGAAGCGGC TGGTGGTCGC TGAGTCAGAG AAGAGTCAGA AGCTGGAAGA GCGGCTGCGC
    CTGCTCCAGG CGGAGACCGC CAGCAGCAGC GCCAGGGCTG CGGAACGCAG CTCCGCTCTG
    CGTGAGGAGG TCCAGACCCT CCGGGAGGAG GCTGAGAAGC AGCGGGTGGC TTCTGAGAGC
    CTGCGGCAGG AGCTGGCATC GCAGGCGGAG CGAGCAGAGG AGCTGGGCCA AGAGTTAAAG
    GCCTGGCAGG AGAAGTTCTT TCAGAAGGAG CAAGCCCTCT CGGCCCTGCA GCTGGAGCAC
    ACCAGCACAC AGGCCCTGGC GAGCGAGCTG CTGCCCGTCA AGCACCTGTG CCAGCAGCTG
    CAGGCCGAGC AGGCTGCTGC CGAGAAGCGC CACCGGGAGG AGCTGGAGCA GAGCAAGCAG
    GCGGCTGGCG GGCTGCGAGC AGAGCTGCTG CGGGCCCAGC GCGAGCTCGG GGAGTTGCTG
    CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG CGCATGGCCC AGCAGCTGCG AGCGGAGAAG
    GCCAGCTACG CGGAGCAACT GAGCATGCTG AAGAAGGCTC ACGGCCTCTT GGCGGAGGAG
    AACCGCGGGC TGGGCGAGCG GGCCAGCCTC GGCCGGCAGT TTCTGGAAGT GGAGCTGGAC
    CAGGCCCGCG AGAAGTACAG CCAGGAGCTG GCAGCTGTGC GCGCTGACGC TGAGACCCGT
    CTGGCTGAGA TGCAGCGGGA AGTGCAGAGC ACTGCCCGGG AGCTGGAGGT GATGACCGCC
    AAGTACGAGG GTGCCAAGGC CAAGGTCCTG GAGGAGAGGC AGCGTTTCCA GGAGGAGAGG
    CAGAAACTCA CTGCCCAGGT GGAGCAGCTA GAGTTATTTC AGAGAGAGCA GACTAAACAG
    GTGGAAGAAC TGAGCAAGAA GCTAGCTGAC CATGAGCAAG CCAGCAAGGC GCAGCAGCAG
    AAGCTGAAGG CCCAGGGCGG TGAGAGCCAG CAGGAGGTCC AGCGCCTCCA GGCCCAGCTG
    AGTGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCGG CTGAGCACTA CAAGCTACAG
    ATGGAGAAGG CCAAGACTCA TTATGATGCC AAGAAGCAGC AGAACCAAGA GCTGCAGGAG
    CAGCTGCGAG GCCTGGAGCA GCTGCAGACA GAGAACAAGG AGCTGAGGGC AGAAGTGGAT
    CGGCTGGGCC GGGAGCTGCA GCAGGCTGGG CTGAAGACCA AGGAAGCCGA GCAGACCTGC
    CGCCACCTCA CGGCCCAGGT GCGCAGCCTG GAGGCACAGG TTGCCCATGC CGACCAGCAG
    CTTCGGGACC TGGGCAAGTT CCAGGTGGCG ACTGATGCCT TGAAGAGCCG GGAGCCCCAG
    GCTAAGTCTC AGCTGGACTT GAGTATTGAC AGCCTGGATC TGAGCTGCGA GGAGGGGACC
    CCTCTCTCTG TCACCAGCAA GTTGCCTCGC ACCCAGCCAG ACGGCACCAG CATCCCCGGA
    GAGCCAGCCT CCCCCATCTC CCAGCGTCTG CCCCCCAAAG TGGAGTCCCT GGAGAGCCTC
    TACTTCACGC CCATCCCTGC TCGGGGTCAG GCCCCCCTGG AGAGCAGCCT GGACTCCCTG
    GGGGACATCT CCCTGGACTC AAGCCGGAAA ACCCGCTCCG CTCGTAGGCG CACCACGCAG
    ATCATCAACA TCACCATGAC CAAGAAGCTG GACGTGGAGG ATCCAGACAG TGCCAACGCC
    TCCTTCTACA GCACACAGTC AGCCCCCACT TCCCAGGCAG GCCTGAGGGC CGCCTCCTCC
    ACCCAGTCTC TAGCCCGCCT GGGCTCTCCC GACGATGGCA ACTCCACTCT ATTCAGCCTG
    CCTGGCTACC GGCCCACCAC TCGCAGCTCC GCTCGCCGTT CCCAGGCCGG GATGTCCAGT
    GGGGCCCCTC CAGGCAGGAA CAGCTTCTAT GTGGGCACCT GCCAGGACGA GCCCGAACAG
    CTGGATGACT GGAACCGCAT TGCAGAGCTG CAGCAGCGCA ACCGAGTATG CCCACCGCAC
    CTGAAGACCT GCTACCCCCT TGAGTCCAGG CCCTCCCTGA GCCTGCCTGC CATCACAGAT
    GAGGAGATAA AAACCGGCGA CCCCCGGGAG ACCCTGCGCC GAGCCAGCAT GCAGCCAACC
    CAGATAGCTG AGGGCGCTGG CATTACCACA CGGCAGCAGC GCAAGCGGGT TTCCTCAGAG
    ACCCACCAGG GCCCCGGCAC ACCCGAGTCA AAGAAGGCCA CCACATGTTT CCCGCGCCCC
    ATGACCCCCC GGGACCGACA CGAAGGGCGC AGACACAGCA GTACCGAGGC CCAGAAGAAG
    GCAGTTCCAG CTGTTAAACA GGCTGACCGC CGCCAGTCTA TGGCCTTCAG CATCCTCAAC
    ACACCCAAGA AGCTTGGAAA CAGCCTTCTG CGGAAGGCAG CCTCCAAGAA AGCCCCCTCC
    AAAGCCTCAC CCAACCCCCG CAGTGGGACC CGCCGCTCTC CGCGCATCGC CACCACTGCA
    GCCAGCGCTG CCACTGCTGC TGCCAGCGCC GCTGCCACCA CCCCCCGGGC AAAGGGCAAG
    GCGAAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216231.1
    CDS137..6508
    Translation

    Target ORF information:

    RefSeq Version XM_005216174.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216174.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCGC
    CCGCAGTCTC TTGGGACAGC CAGCCGAGAA GACGCTTGTG GATTGCGAAC CCAGTCCGAG
    GCTACTGAAA AGACTGAGCC AGAAGGCTCT GAACTGCAGG CCCTGCAGGC AGAGGTGAGC
    CGGCTGGAAC AGCAGGCCCG TGAGCACCAG GAGAAGGCCT CCAGCCTGGA GCAAAGCCTT
    GAGTCTGAGC GCGCTGCCCA CGCGGAGCAG GCCAGTACTC TGAAGACTCT GCAGGGCCAG
    TTAGAGCAGA AGGCCCAGGA GCTGGGGAGC AGTCAGGACG CCTTAGCCTC AACCCAGAGG
    GAGTTGGCCA CCCTCCGTGC CAAGGCCCAG GAGCACAGCA AGGCCGAGGA CGAGTGGAAG
    GCCCAGGTGG CCCGGGGTCA GCAGGAGGCC GAGAGGAAAA ACAGCCTCAT CAGCAGCTTG
    GAGGAGGAGG TGTCCATCCT GAACCGCCAG GTGCTGGAGA AGGAGGGCGA GAGCAAGGAG
    CTGAAGCGGC TGGTGGTCGC TGAGTCAGAG AAGAGTCAGA AGCTGGAAGA GCGGCTGCGC
    CTGCTCCAGG CGGAGACCGC CAGCAGCAGC GCCAGGGCTG CGGAACGCAG CTCCGCTCTG
    CGTGAGGAGG TCCAGACCCT CCGGGAGGAG GCTGAGAAGC AGCGGGTGGC TTCTGAGAGC
    CTGCGGCAGG AGCTGGCATC GCAGGCGGAG CGAGCAGAGG AGCTGGGCCA AGAGTTAAAG
    GCCTGGCAGG AGAAGTTCTT TCAGAAGGAG CAAGCCCTCT CGGCCCTGCA GCTGGAGCAC
    ACCAGCACAC AGGCCCTGGC GAGCGAGCTG CTGCCCGTCA AGCACCTGTG CCAGCAGCTG
    CAGGCCGAGC AGGCTGCTGC CGAGAAGCGC CACCGGGAGG AGCTGGAGCA GAGCAAGCAG
    GCGGCTGGCG GGCTGCGAGC AGAGCTGCTG CGGGCCCAGC GCGAGCTCGG GGAGTTGCTG
    CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG CGCATGGCCC AGCAGCTGCG AGCGGAGAAG
    GCCAGCTACG CGGAGCAACT GAGCATGCTG AAGAAGGCTC ACGGCCTCTT GGCGGAGGAG
    AACCGCGGGC TGGGCGAGCG GGCCAGCCTC GGCCGGCAGT TTCTGGAAGT GGAGCTGGAC
    CAGGCCCGCG AGAAGTACAG CCAGGAGCTG GCAGCTGTGC GCGCTGACGC TGAGACCCGT
    CTGGCTGAGA TGCAGCGGGA AGTGCAGAGC ACTGCCCGGG AGCTGGAGGT GATGACCGCC
    AAGTACGAGG GTGCCAAGGC CAAGGTCCTG GAGGAGAGGC AGCGTTTCCA GGAGGAGAGG
    CAGAAACTCA CTGCCCAGGT GGAGCAGCTA GAGTTATTTC AGAGAGAGCA GACTAAACAG
    GTGGAAGAAC TGAGCAAGAA GCTAGCTGAC CATGAGCAAG CCAGCAAGGC GCAGCAGCAG
    AAGCTGAAGG CCCAGGGCGG TGAGAGCCAG CAGGAGGTCC AGCGCCTCCA GGCCCAGCTG
    AGTGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCGG CTGAGCACTA CAAGCTACAG
    ATGGAGAAGG CCAAGACTCA TTATGATGCC AAGAAGCAGC AGAACCAAGA GCTGCAGGAG
    CAGCTGCGAG GCCTGGAGCA GCTGCAGACA GAGAACAAGG AGCTGAGGGC AGAAGTGGAT
    CGGCTGGGCC GGGAGCTGCA GCAGGCTGGG CTGAAGACCA AGGAAGCCGA GCAGACCTGC
    CGCCACCTCA CGGCCCAGGT GCGCAGCCTG GAGGCACAGG TTGCCCATGC CGACCAGCAG
    CTTCGGGACC TGGGCAAGTT CCAGGTGGCG ACTGATGCCT TGAAGAGCCG GGAGCCCCAG
    GCTAAGTCTC AGCTGGACTT GAGTATTGAC AGCCTGGATC TGAGCTGCGA GGAGGGGACC
    CCTCTCTCTG TCACCAGCAA GTTGCCTCGC ACCCAGCCAG ACGGCACCAG CATCCCCGGA
    GAGCCAGCCT CCCCCATCTC CCAGCGTCTG CCCCCCAAAG TGGAGTCCCT GGAGAGCCTC
    TACTTCACGC CCATCCCTGC TCGGGGTCAG GCCCCCCTGG AGAGCAGCCT GGACTCCCTG
    GGGGACATCT CCCTGGACTC AAGCCGGAAA ACCCGCTCCG CTCGTAGGCG CACCACGCAG
    ATCATCAACA TCACCATGAC CAAGAAGCTG GACGTGGAGG ATCCAGACAG TGCCAACGCC
    TCCTTCTACA GCACACAGTC AGCCCCCACT TCCCAGGCAG GCCTGAGGGC CGCCTCCTCC
    ACCCAGTCTC TAGCCCGCCT GGGCTCTCCC GACGATGGCA ACTCCACTCT ATTCAGCCTG
    CCTGGCTACC GGCCCACCAC TCGCAGCTCC GCTCGCCGTT CCCAGGCCGG GATGTCCAGT
    GGGGCCCCTC CAGGCAGGAA CAGCTTCTAT GTGGGCACCT GCCAGGACGA GCCCGAACAG
    CTGGATGACT GGAACCGCAT TGCAGAGCTG CAGCAGCGCA ACCGAGTATG CCCACCGCAC
    CTGAAGACCT GCTACCCCCT TGAGTCCAGG CCCTCCCTGA GCCTGCCTGC CATCACAGAT
    GAGGAGATAA AAACCGGCGA CCCCCGGGAG ACCCTGCGCC GAGCCAGCAT GCAGCCAACC
    CAGATAGCTG AGGGCGCTGG CATTACCACA CGGCAGCAGC GCAAGCGGGT TTCCTCAGAG
    ACCCACCAGG GCCCCGGCAC ACCCGAGTCA AAGAAGGCCA CCACATGTTT CCCGCGCCCC
    ATGACCCCCC GGGACCGACA CGAAGGGCGC AGACACAGCA GTACCGAGGC CCAGAAGAAG
    GCAGTTCCAG CTGTTAAACA GGCTGACCGC CGCCAGTCTA TGGCCTTCAG CATCCTCAAC
    ACACCCAAGA AGCTTGGAAA CAGCCTTCTG CGGAAGGCAG CCTCCAAGAA AGCCCCCTCC
    AAAGCCTCAC CCAACCCCCG CAGTGGGACC CGCCGCTCTC CGCGCATCGC CACCACTGCA
    GCCAGCGCTG CCACTGCTGC TGCCAGCGCC GCTGCCACCA CCCCCCGGGC AAAGGGCAAG
    GCGAAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26522
    Clone ID Related Accession (Same CDS sequence) XM_005216174.3 , XM_010812655.2 , XM_005216172.1 , XM_015474764.1 , XM_005216171.3 , XM_015474763.1 , XM_005216173.3
    Accession Version XM_010812655.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear mitotic apparatus protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104415.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCGC
    CCGCAGTCTC TTGGGACAGC CAGCCGAGAA GACGCTTGTG GATTGCGAAC CCAGTCCGAG
    GCTACTGAAA AGACTGAGCC AGAAGGCTCT GAACTGCAGG CCCTGCAGGC AGAGGTGAGC
    CGGCTGGAAC AGCAGGCCCG TGAGCACCAG GAGAAGGCCT CCAGCCTGGA GCAAAGCCTT
    GAGTCTGAGC GCGCTGCCCA CGCGGAGCAG GCCAGTACTC TGAAGACTCT GCAGGGCCAG
    TTAGAGCAGA AGGCCCAGGA GCTGGGGAGC AGTCAGGACG CCTTAGCCTC AACCCAGAGG
    GAGTTGGCCA CCCTCCGTGC CAAGGCCCAG GAGCACAGCA AGGCCGAGGA CGAGTGGAAG
    GCCCAGGTGG CCCGGGGTCA GCAGGAGGCC GAGAGGAAAA ACAGCCTCAT CAGCAGCTTG
    GAGGAGGAGG TGTCCATCCT GAACCGCCAG GTGCTGGAGA AGGAGGGCGA GAGCAAGGAG
    CTGAAGCGGC TGGTGGTCGC TGAGTCAGAG AAGAGTCAGA AGCTGGAAGA GCGGCTGCGC
    CTGCTCCAGG CGGAGACCGC CAGCAGCAGC GCCAGGGCTG CGGAACGCAG CTCCGCTCTG
    CGTGAGGAGG TCCAGACCCT CCGGGAGGAG GCTGAGAAGC AGCGGGTGGC TTCTGAGAGC
    CTGCGGCAGG AGCTGGCATC GCAGGCGGAG CGAGCAGAGG AGCTGGGCCA AGAGTTAAAG
    GCCTGGCAGG AGAAGTTCTT TCAGAAGGAG CAAGCCCTCT CGGCCCTGCA GCTGGAGCAC
    ACCAGCACAC AGGCCCTGGC GAGCGAGCTG CTGCCCGTCA AGCACCTGTG CCAGCAGCTG
    CAGGCCGAGC AGGCTGCTGC CGAGAAGCGC CACCGGGAGG AGCTGGAGCA GAGCAAGCAG
    GCGGCTGGCG GGCTGCGAGC AGAGCTGCTG CGGGCCCAGC GCGAGCTCGG GGAGTTGCTG
    CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG CGCATGGCCC AGCAGCTGCG AGCGGAGAAG
    GCCAGCTACG CGGAGCAACT GAGCATGCTG AAGAAGGCTC ACGGCCTCTT GGCGGAGGAG
    AACCGCGGGC TGGGCGAGCG GGCCAGCCTC GGCCGGCAGT TTCTGGAAGT GGAGCTGGAC
    CAGGCCCGCG AGAAGTACAG CCAGGAGCTG GCAGCTGTGC GCGCTGACGC TGAGACCCGT
    CTGGCTGAGA TGCAGCGGGA AGTGCAGAGC ACTGCCCGGG AGCTGGAGGT GATGACCGCC
    AAGTACGAGG GTGCCAAGGC CAAGGTCCTG GAGGAGAGGC AGCGTTTCCA GGAGGAGAGG
    CAGAAACTCA CTGCCCAGGT GGAGCAGCTA GAGTTATTTC AGAGAGAGCA GACTAAACAG
    GTGGAAGAAC TGAGCAAGAA GCTAGCTGAC CATGAGCAAG CCAGCAAGGC GCAGCAGCAG
    AAGCTGAAGG CCCAGGGCGG TGAGAGCCAG CAGGAGGTCC AGCGCCTCCA GGCCCAGCTG
    AGTGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCGG CTGAGCACTA CAAGCTACAG
    ATGGAGAAGG CCAAGACTCA TTATGATGCC AAGAAGCAGC AGAACCAAGA GCTGCAGGAG
    CAGCTGCGAG GCCTGGAGCA GCTGCAGACA GAGAACAAGG AGCTGAGGGC AGAAGTGGAT
    CGGCTGGGCC GGGAGCTGCA GCAGGCTGGG CTGAAGACCA AGGAAGCCGA GCAGACCTGC
    CGCCACCTCA CGGCCCAGGT GCGCAGCCTG GAGGCACAGG TTGCCCATGC CGACCAGCAG
    CTTCGGGACC TGGGCAAGTT CCAGGTGGCG ACTGATGCCT TGAAGAGCCG GGAGCCCCAG
    GCTAAGTCTC AGCTGGACTT GAGTATTGAC AGCCTGGATC TGAGCTGCGA GGAGGGGACC
    CCTCTCTCTG TCACCAGCAA GTTGCCTCGC ACCCAGCCAG ACGGCACCAG CATCCCCGGA
    GAGCCAGCCT CCCCCATCTC CCAGCGTCTG CCCCCCAAAG TGGAGTCCCT GGAGAGCCTC
    TACTTCACGC CCATCCCTGC TCGGGGTCAG GCCCCCCTGG AGAGCAGCCT GGACTCCCTG
    GGGGACATCT CCCTGGACTC AAGCCGGAAA ACCCGCTCCG CTCGTAGGCG CACCACGCAG
    ATCATCAACA TCACCATGAC CAAGAAGCTG GACGTGGAGG ATCCAGACAG TGCCAACGCC
    TCCTTCTACA GCACACAGTC AGCCCCCACT TCCCAGGCAG GCCTGAGGGC CGCCTCCTCC
    ACCCAGTCTC TAGCCCGCCT GGGCTCTCCC GACGATGGCA ACTCCACTCT ATTCAGCCTG
    CCTGGCTACC GGCCCACCAC TCGCAGCTCC GCTCGCCGTT CCCAGGCCGG GATGTCCAGT
    GGGGCCCCTC CAGGCAGGAA CAGCTTCTAT GTGGGCACCT GCCAGGACGA GCCCGAACAG
    CTGGATGACT GGAACCGCAT TGCAGAGCTG CAGCAGCGCA ACCGAGTATG CCCACCGCAC
    CTGAAGACCT GCTACCCCCT TGAGTCCAGG CCCTCCCTGA GCCTGCCTGC CATCACAGAT
    GAGGAGATAA AAACCGGCGA CCCCCGGGAG ACCCTGCGCC GAGCCAGCAT GCAGCCAACC
    CAGATAGCTG AGGGCGCTGG CATTACCACA CGGCAGCAGC GCAAGCGGGT TTCCTCAGAG
    ACCCACCAGG GCCCCGGCAC ACCCGAGTCA AAGAAGGCCA CCACATGTTT CCCGCGCCCC
    ATGACCCCCC GGGACCGACA CGAAGGGCGC AGACACAGCA GTACCGAGGC CCAGAAGAAG
    GCAGTTCCAG CTGTTAAACA GGCTGACCGC CGCCAGTCTA TGGCCTTCAG CATCCTCAAC
    ACACCCAAGA AGCTTGGAAA CAGCCTTCTG CGGAAGGCAG CCTCCAAGAA AGCCCCCTCC
    AAAGCCTCAC CCAACCCCCG CAGTGGGACC CGCCGCTCTC CGCGCATCGC CACCACTGCA
    GCCAGCGCTG CCACTGCTGC TGCCAGCGCC GCTGCCACCA CCCCCCGGGC AAAGGGCAAG
    GCGAAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810957.1
    CDS149..6520
    Translation

    Target ORF information:

    RefSeq Version XM_010812655.2
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812655.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCGC
    CCGCAGTCTC TTGGGACAGC CAGCCGAGAA GACGCTTGTG GATTGCGAAC CCAGTCCGAG
    GCTACTGAAA AGACTGAGCC AGAAGGCTCT GAACTGCAGG CCCTGCAGGC AGAGGTGAGC
    CGGCTGGAAC AGCAGGCCCG TGAGCACCAG GAGAAGGCCT CCAGCCTGGA GCAAAGCCTT
    GAGTCTGAGC GCGCTGCCCA CGCGGAGCAG GCCAGTACTC TGAAGACTCT GCAGGGCCAG
    TTAGAGCAGA AGGCCCAGGA GCTGGGGAGC AGTCAGGACG CCTTAGCCTC AACCCAGAGG
    GAGTTGGCCA CCCTCCGTGC CAAGGCCCAG GAGCACAGCA AGGCCGAGGA CGAGTGGAAG
    GCCCAGGTGG CCCGGGGTCA GCAGGAGGCC GAGAGGAAAA ACAGCCTCAT CAGCAGCTTG
    GAGGAGGAGG TGTCCATCCT GAACCGCCAG GTGCTGGAGA AGGAGGGCGA GAGCAAGGAG
    CTGAAGCGGC TGGTGGTCGC TGAGTCAGAG AAGAGTCAGA AGCTGGAAGA GCGGCTGCGC
    CTGCTCCAGG CGGAGACCGC CAGCAGCAGC GCCAGGGCTG CGGAACGCAG CTCCGCTCTG
    CGTGAGGAGG TCCAGACCCT CCGGGAGGAG GCTGAGAAGC AGCGGGTGGC TTCTGAGAGC
    CTGCGGCAGG AGCTGGCATC GCAGGCGGAG CGAGCAGAGG AGCTGGGCCA AGAGTTAAAG
    GCCTGGCAGG AGAAGTTCTT TCAGAAGGAG CAAGCCCTCT CGGCCCTGCA GCTGGAGCAC
    ACCAGCACAC AGGCCCTGGC GAGCGAGCTG CTGCCCGTCA AGCACCTGTG CCAGCAGCTG
    CAGGCCGAGC AGGCTGCTGC CGAGAAGCGC CACCGGGAGG AGCTGGAGCA GAGCAAGCAG
    GCGGCTGGCG GGCTGCGAGC AGAGCTGCTG CGGGCCCAGC GCGAGCTCGG GGAGTTGCTG
    CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG CGCATGGCCC AGCAGCTGCG AGCGGAGAAG
    GCCAGCTACG CGGAGCAACT GAGCATGCTG AAGAAGGCTC ACGGCCTCTT GGCGGAGGAG
    AACCGCGGGC TGGGCGAGCG GGCCAGCCTC GGCCGGCAGT TTCTGGAAGT GGAGCTGGAC
    CAGGCCCGCG AGAAGTACAG CCAGGAGCTG GCAGCTGTGC GCGCTGACGC TGAGACCCGT
    CTGGCTGAGA TGCAGCGGGA AGTGCAGAGC ACTGCCCGGG AGCTGGAGGT GATGACCGCC
    AAGTACGAGG GTGCCAAGGC CAAGGTCCTG GAGGAGAGGC AGCGTTTCCA GGAGGAGAGG
    CAGAAACTCA CTGCCCAGGT GGAGCAGCTA GAGTTATTTC AGAGAGAGCA GACTAAACAG
    GTGGAAGAAC TGAGCAAGAA GCTAGCTGAC CATGAGCAAG CCAGCAAGGC GCAGCAGCAG
    AAGCTGAAGG CCCAGGGCGG TGAGAGCCAG CAGGAGGTCC AGCGCCTCCA GGCCCAGCTG
    AGTGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCGG CTGAGCACTA CAAGCTACAG
    ATGGAGAAGG CCAAGACTCA TTATGATGCC AAGAAGCAGC AGAACCAAGA GCTGCAGGAG
    CAGCTGCGAG GCCTGGAGCA GCTGCAGACA GAGAACAAGG AGCTGAGGGC AGAAGTGGAT
    CGGCTGGGCC GGGAGCTGCA GCAGGCTGGG CTGAAGACCA AGGAAGCCGA GCAGACCTGC
    CGCCACCTCA CGGCCCAGGT GCGCAGCCTG GAGGCACAGG TTGCCCATGC CGACCAGCAG
    CTTCGGGACC TGGGCAAGTT CCAGGTGGCG ACTGATGCCT TGAAGAGCCG GGAGCCCCAG
    GCTAAGTCTC AGCTGGACTT GAGTATTGAC AGCCTGGATC TGAGCTGCGA GGAGGGGACC
    CCTCTCTCTG TCACCAGCAA GTTGCCTCGC ACCCAGCCAG ACGGCACCAG CATCCCCGGA
    GAGCCAGCCT CCCCCATCTC CCAGCGTCTG CCCCCCAAAG TGGAGTCCCT GGAGAGCCTC
    TACTTCACGC CCATCCCTGC TCGGGGTCAG GCCCCCCTGG AGAGCAGCCT GGACTCCCTG
    GGGGACATCT CCCTGGACTC AAGCCGGAAA ACCCGCTCCG CTCGTAGGCG CACCACGCAG
    ATCATCAACA TCACCATGAC CAAGAAGCTG GACGTGGAGG ATCCAGACAG TGCCAACGCC
    TCCTTCTACA GCACACAGTC AGCCCCCACT TCCCAGGCAG GCCTGAGGGC CGCCTCCTCC
    ACCCAGTCTC TAGCCCGCCT GGGCTCTCCC GACGATGGCA ACTCCACTCT ATTCAGCCTG
    CCTGGCTACC GGCCCACCAC TCGCAGCTCC GCTCGCCGTT CCCAGGCCGG GATGTCCAGT
    GGGGCCCCTC CAGGCAGGAA CAGCTTCTAT GTGGGCACCT GCCAGGACGA GCCCGAACAG
    CTGGATGACT GGAACCGCAT TGCAGAGCTG CAGCAGCGCA ACCGAGTATG CCCACCGCAC
    CTGAAGACCT GCTACCCCCT TGAGTCCAGG CCCTCCCTGA GCCTGCCTGC CATCACAGAT
    GAGGAGATAA AAACCGGCGA CCCCCGGGAG ACCCTGCGCC GAGCCAGCAT GCAGCCAACC
    CAGATAGCTG AGGGCGCTGG CATTACCACA CGGCAGCAGC GCAAGCGGGT TTCCTCAGAG
    ACCCACCAGG GCCCCGGCAC ACCCGAGTCA AAGAAGGCCA CCACATGTTT CCCGCGCCCC
    ATGACCCCCC GGGACCGACA CGAAGGGCGC AGACACAGCA GTACCGAGGC CCAGAAGAAG
    GCAGTTCCAG CTGTTAAACA GGCTGACCGC CGCCAGTCTA TGGCCTTCAG CATCCTCAAC
    ACACCCAAGA AGCTTGGAAA CAGCCTTCTG CGGAAGGCAG CCTCCAAGAA AGCCCCCTCC
    AAAGCCTCAC CCAACCCCCG CAGTGGGACC CGCCGCTCTC CGCGCATCGC CACCACTGCA
    GCCAGCGCTG CCACTGCTGC TGCCAGCGCC GCTGCCACCA CCCCCCGGGC AAAGGGCAAG
    GCGAAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26522
    Clone ID Related Accession (Same CDS sequence) XM_005216174.3 , XM_010812655.2 , XM_005216172.1 , XM_015474764.1 , XM_005216171.3 , XM_015474763.1 , XM_005216173.3
    Accession Version XM_005216172.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear mitotic apparatus protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104415.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCGC
    CCGCAGTCTC TTGGGACAGC CAGCCGAGAA GACGCTTGTG GATTGCGAAC CCAGTCCGAG
    GCTACTGAAA AGACTGAGCC AGAAGGCTCT GAACTGCAGG CCCTGCAGGC AGAGGTGAGC
    CGGCTGGAAC AGCAGGCCCG TGAGCACCAG GAGAAGGCCT CCAGCCTGGA GCAAAGCCTT
    GAGTCTGAGC GCGCTGCCCA CGCGGAGCAG GCCAGTACTC TGAAGACTCT GCAGGGCCAG
    TTAGAGCAGA AGGCCCAGGA GCTGGGGAGC AGTCAGGACG CCTTAGCCTC AACCCAGAGG
    GAGTTGGCCA CCCTCCGTGC CAAGGCCCAG GAGCACAGCA AGGCCGAGGA CGAGTGGAAG
    GCCCAGGTGG CCCGGGGTCA GCAGGAGGCC GAGAGGAAAA ACAGCCTCAT CAGCAGCTTG
    GAGGAGGAGG TGTCCATCCT GAACCGCCAG GTGCTGGAGA AGGAGGGCGA GAGCAAGGAG
    CTGAAGCGGC TGGTGGTCGC TGAGTCAGAG AAGAGTCAGA AGCTGGAAGA GCGGCTGCGC
    CTGCTCCAGG CGGAGACCGC CAGCAGCAGC GCCAGGGCTG CGGAACGCAG CTCCGCTCTG
    CGTGAGGAGG TCCAGACCCT CCGGGAGGAG GCTGAGAAGC AGCGGGTGGC TTCTGAGAGC
    CTGCGGCAGG AGCTGGCATC GCAGGCGGAG CGAGCAGAGG AGCTGGGCCA AGAGTTAAAG
    GCCTGGCAGG AGAAGTTCTT TCAGAAGGAG CAAGCCCTCT CGGCCCTGCA GCTGGAGCAC
    ACCAGCACAC AGGCCCTGGC GAGCGAGCTG CTGCCCGTCA AGCACCTGTG CCAGCAGCTG
    CAGGCCGAGC AGGCTGCTGC CGAGAAGCGC CACCGGGAGG AGCTGGAGCA GAGCAAGCAG
    GCGGCTGGCG GGCTGCGAGC AGAGCTGCTG CGGGCCCAGC GCGAGCTCGG GGAGTTGCTG
    CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG CGCATGGCCC AGCAGCTGCG AGCGGAGAAG
    GCCAGCTACG CGGAGCAACT GAGCATGCTG AAGAAGGCTC ACGGCCTCTT GGCGGAGGAG
    AACCGCGGGC TGGGCGAGCG GGCCAGCCTC GGCCGGCAGT TTCTGGAAGT GGAGCTGGAC
    CAGGCCCGCG AGAAGTACAG CCAGGAGCTG GCAGCTGTGC GCGCTGACGC TGAGACCCGT
    CTGGCTGAGA TGCAGCGGGA AGTGCAGAGC ACTGCCCGGG AGCTGGAGGT GATGACCGCC
    AAGTACGAGG GTGCCAAGGC CAAGGTCCTG GAGGAGAGGC AGCGTTTCCA GGAGGAGAGG
    CAGAAACTCA CTGCCCAGGT GGAGCAGCTA GAGTTATTTC AGAGAGAGCA GACTAAACAG
    GTGGAAGAAC TGAGCAAGAA GCTAGCTGAC CATGAGCAAG CCAGCAAGGC GCAGCAGCAG
    AAGCTGAAGG CCCAGGGCGG TGAGAGCCAG CAGGAGGTCC AGCGCCTCCA GGCCCAGCTG
    AGTGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCGG CTGAGCACTA CAAGCTACAG
    ATGGAGAAGG CCAAGACTCA TTATGATGCC AAGAAGCAGC AGAACCAAGA GCTGCAGGAG
    CAGCTGCGAG GCCTGGAGCA GCTGCAGACA GAGAACAAGG AGCTGAGGGC AGAAGTGGAT
    CGGCTGGGCC GGGAGCTGCA GCAGGCTGGG CTGAAGACCA AGGAAGCCGA GCAGACCTGC
    CGCCACCTCA CGGCCCAGGT GCGCAGCCTG GAGGCACAGG TTGCCCATGC CGACCAGCAG
    CTTCGGGACC TGGGCAAGTT CCAGGTGGCG ACTGATGCCT TGAAGAGCCG GGAGCCCCAG
    GCTAAGTCTC AGCTGGACTT GAGTATTGAC AGCCTGGATC TGAGCTGCGA GGAGGGGACC
    CCTCTCTCTG TCACCAGCAA GTTGCCTCGC ACCCAGCCAG ACGGCACCAG CATCCCCGGA
    GAGCCAGCCT CCCCCATCTC CCAGCGTCTG CCCCCCAAAG TGGAGTCCCT GGAGAGCCTC
    TACTTCACGC CCATCCCTGC TCGGGGTCAG GCCCCCCTGG AGAGCAGCCT GGACTCCCTG
    GGGGACATCT CCCTGGACTC AAGCCGGAAA ACCCGCTCCG CTCGTAGGCG CACCACGCAG
    ATCATCAACA TCACCATGAC CAAGAAGCTG GACGTGGAGG ATCCAGACAG TGCCAACGCC
    TCCTTCTACA GCACACAGTC AGCCCCCACT TCCCAGGCAG GCCTGAGGGC CGCCTCCTCC
    ACCCAGTCTC TAGCCCGCCT GGGCTCTCCC GACGATGGCA ACTCCACTCT ATTCAGCCTG
    CCTGGCTACC GGCCCACCAC TCGCAGCTCC GCTCGCCGTT CCCAGGCCGG GATGTCCAGT
    GGGGCCCCTC CAGGCAGGAA CAGCTTCTAT GTGGGCACCT GCCAGGACGA GCCCGAACAG
    CTGGATGACT GGAACCGCAT TGCAGAGCTG CAGCAGCGCA ACCGAGTATG CCCACCGCAC
    CTGAAGACCT GCTACCCCCT TGAGTCCAGG CCCTCCCTGA GCCTGCCTGC CATCACAGAT
    GAGGAGATAA AAACCGGCGA CCCCCGGGAG ACCCTGCGCC GAGCCAGCAT GCAGCCAACC
    CAGATAGCTG AGGGCGCTGG CATTACCACA CGGCAGCAGC GCAAGCGGGT TTCCTCAGAG
    ACCCACCAGG GCCCCGGCAC ACCCGAGTCA AAGAAGGCCA CCACATGTTT CCCGCGCCCC
    ATGACCCCCC GGGACCGACA CGAAGGGCGC AGACACAGCA GTACCGAGGC CCAGAAGAAG
    GCAGTTCCAG CTGTTAAACA GGCTGACCGC CGCCAGTCTA TGGCCTTCAG CATCCTCAAC
    ACACCCAAGA AGCTTGGAAA CAGCCTTCTG CGGAAGGCAG CCTCCAAGAA AGCCCCCTCC
    AAAGCCTCAC CCAACCCCCG CAGTGGGACC CGCCGCTCTC CGCGCATCGC CACCACTGCA
    GCCAGCGCTG CCACTGCTGC TGCCAGCGCC GCTGCCACCA CCCCCCGGGC AAAGGGCAAG
    GCGAAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216229.1
    CDS340..6711
    Translation

    Target ORF information:

    RefSeq Version XM_005216172.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216172.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCGC
    CCGCAGTCTC TTGGGACAGC CAGCCGAGAA GACGCTTGTG GATTGCGAAC CCAGTCCGAG
    GCTACTGAAA AGACTGAGCC AGAAGGCTCT GAACTGCAGG CCCTGCAGGC AGAGGTGAGC
    CGGCTGGAAC AGCAGGCCCG TGAGCACCAG GAGAAGGCCT CCAGCCTGGA GCAAAGCCTT
    GAGTCTGAGC GCGCTGCCCA CGCGGAGCAG GCCAGTACTC TGAAGACTCT GCAGGGCCAG
    TTAGAGCAGA AGGCCCAGGA GCTGGGGAGC AGTCAGGACG CCTTAGCCTC AACCCAGAGG
    GAGTTGGCCA CCCTCCGTGC CAAGGCCCAG GAGCACAGCA AGGCCGAGGA CGAGTGGAAG
    GCCCAGGTGG CCCGGGGTCA GCAGGAGGCC GAGAGGAAAA ACAGCCTCAT CAGCAGCTTG
    GAGGAGGAGG TGTCCATCCT GAACCGCCAG GTGCTGGAGA AGGAGGGCGA GAGCAAGGAG
    CTGAAGCGGC TGGTGGTCGC TGAGTCAGAG AAGAGTCAGA AGCTGGAAGA GCGGCTGCGC
    CTGCTCCAGG CGGAGACCGC CAGCAGCAGC GCCAGGGCTG CGGAACGCAG CTCCGCTCTG
    CGTGAGGAGG TCCAGACCCT CCGGGAGGAG GCTGAGAAGC AGCGGGTGGC TTCTGAGAGC
    CTGCGGCAGG AGCTGGCATC GCAGGCGGAG CGAGCAGAGG AGCTGGGCCA AGAGTTAAAG
    GCCTGGCAGG AGAAGTTCTT TCAGAAGGAG CAAGCCCTCT CGGCCCTGCA GCTGGAGCAC
    ACCAGCACAC AGGCCCTGGC GAGCGAGCTG CTGCCCGTCA AGCACCTGTG CCAGCAGCTG
    CAGGCCGAGC AGGCTGCTGC CGAGAAGCGC CACCGGGAGG AGCTGGAGCA GAGCAAGCAG
    GCGGCTGGCG GGCTGCGAGC AGAGCTGCTG CGGGCCCAGC GCGAGCTCGG GGAGTTGCTG
    CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG CGCATGGCCC AGCAGCTGCG AGCGGAGAAG
    GCCAGCTACG CGGAGCAACT GAGCATGCTG AAGAAGGCTC ACGGCCTCTT GGCGGAGGAG
    AACCGCGGGC TGGGCGAGCG GGCCAGCCTC GGCCGGCAGT TTCTGGAAGT GGAGCTGGAC
    CAGGCCCGCG AGAAGTACAG CCAGGAGCTG GCAGCTGTGC GCGCTGACGC TGAGACCCGT
    CTGGCTGAGA TGCAGCGGGA AGTGCAGAGC ACTGCCCGGG AGCTGGAGGT GATGACCGCC
    AAGTACGAGG GTGCCAAGGC CAAGGTCCTG GAGGAGAGGC AGCGTTTCCA GGAGGAGAGG
    CAGAAACTCA CTGCCCAGGT GGAGCAGCTA GAGTTATTTC AGAGAGAGCA GACTAAACAG
    GTGGAAGAAC TGAGCAAGAA GCTAGCTGAC CATGAGCAAG CCAGCAAGGC GCAGCAGCAG
    AAGCTGAAGG CCCAGGGCGG TGAGAGCCAG CAGGAGGTCC AGCGCCTCCA GGCCCAGCTG
    AGTGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCGG CTGAGCACTA CAAGCTACAG
    ATGGAGAAGG CCAAGACTCA TTATGATGCC AAGAAGCAGC AGAACCAAGA GCTGCAGGAG
    CAGCTGCGAG GCCTGGAGCA GCTGCAGACA GAGAACAAGG AGCTGAGGGC AGAAGTGGAT
    CGGCTGGGCC GGGAGCTGCA GCAGGCTGGG CTGAAGACCA AGGAAGCCGA GCAGACCTGC
    CGCCACCTCA CGGCCCAGGT GCGCAGCCTG GAGGCACAGG TTGCCCATGC CGACCAGCAG
    CTTCGGGACC TGGGCAAGTT CCAGGTGGCG ACTGATGCCT TGAAGAGCCG GGAGCCCCAG
    GCTAAGTCTC AGCTGGACTT GAGTATTGAC AGCCTGGATC TGAGCTGCGA GGAGGGGACC
    CCTCTCTCTG TCACCAGCAA GTTGCCTCGC ACCCAGCCAG ACGGCACCAG CATCCCCGGA
    GAGCCAGCCT CCCCCATCTC CCAGCGTCTG CCCCCCAAAG TGGAGTCCCT GGAGAGCCTC
    TACTTCACGC CCATCCCTGC TCGGGGTCAG GCCCCCCTGG AGAGCAGCCT GGACTCCCTG
    GGGGACATCT CCCTGGACTC AAGCCGGAAA ACCCGCTCCG CTCGTAGGCG CACCACGCAG
    ATCATCAACA TCACCATGAC CAAGAAGCTG GACGTGGAGG ATCCAGACAG TGCCAACGCC
    TCCTTCTACA GCACACAGTC AGCCCCCACT TCCCAGGCAG GCCTGAGGGC CGCCTCCTCC
    ACCCAGTCTC TAGCCCGCCT GGGCTCTCCC GACGATGGCA ACTCCACTCT ATTCAGCCTG
    CCTGGCTACC GGCCCACCAC TCGCAGCTCC GCTCGCCGTT CCCAGGCCGG GATGTCCAGT
    GGGGCCCCTC CAGGCAGGAA CAGCTTCTAT GTGGGCACCT GCCAGGACGA GCCCGAACAG
    CTGGATGACT GGAACCGCAT TGCAGAGCTG CAGCAGCGCA ACCGAGTATG CCCACCGCAC
    CTGAAGACCT GCTACCCCCT TGAGTCCAGG CCCTCCCTGA GCCTGCCTGC CATCACAGAT
    GAGGAGATAA AAACCGGCGA CCCCCGGGAG ACCCTGCGCC GAGCCAGCAT GCAGCCAACC
    CAGATAGCTG AGGGCGCTGG CATTACCACA CGGCAGCAGC GCAAGCGGGT TTCCTCAGAG
    ACCCACCAGG GCCCCGGCAC ACCCGAGTCA AAGAAGGCCA CCACATGTTT CCCGCGCCCC
    ATGACCCCCC GGGACCGACA CGAAGGGCGC AGACACAGCA GTACCGAGGC CCAGAAGAAG
    GCAGTTCCAG CTGTTAAACA GGCTGACCGC CGCCAGTCTA TGGCCTTCAG CATCCTCAAC
    ACACCCAAGA AGCTTGGAAA CAGCCTTCTG CGGAAGGCAG CCTCCAAGAA AGCCCCCTCC
    AAAGCCTCAC CCAACCCCCG CAGTGGGACC CGCCGCTCTC CGCGCATCGC CACCACTGCA
    GCCAGCGCTG CCACTGCTGC TGCCAGCGCC GCTGCCACCA CCCCCCGGGC AAAGGGCAAG
    GCGAAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26522
    Clone ID Related Accession (Same CDS sequence) XM_005216174.3 , XM_010812655.2 , XM_005216172.1 , XM_015474764.1 , XM_005216171.3 , XM_015474763.1 , XM_005216173.3
    Accession Version XM_015474764.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear mitotic apparatus protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104415.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCGC
    CCGCAGTCTC TTGGGACAGC CAGCCGAGAA GACGCTTGTG GATTGCGAAC CCAGTCCGAG
    GCTACTGAAA AGACTGAGCC AGAAGGCTCT GAACTGCAGG CCCTGCAGGC AGAGGTGAGC
    CGGCTGGAAC AGCAGGCCCG TGAGCACCAG GAGAAGGCCT CCAGCCTGGA GCAAAGCCTT
    GAGTCTGAGC GCGCTGCCCA CGCGGAGCAG GCCAGTACTC TGAAGACTCT GCAGGGCCAG
    TTAGAGCAGA AGGCCCAGGA GCTGGGGAGC AGTCAGGACG CCTTAGCCTC AACCCAGAGG
    GAGTTGGCCA CCCTCCGTGC CAAGGCCCAG GAGCACAGCA AGGCCGAGGA CGAGTGGAAG
    GCCCAGGTGG CCCGGGGTCA GCAGGAGGCC GAGAGGAAAA ACAGCCTCAT CAGCAGCTTG
    GAGGAGGAGG TGTCCATCCT GAACCGCCAG GTGCTGGAGA AGGAGGGCGA GAGCAAGGAG
    CTGAAGCGGC TGGTGGTCGC TGAGTCAGAG AAGAGTCAGA AGCTGGAAGA GCGGCTGCGC
    CTGCTCCAGG CGGAGACCGC CAGCAGCAGC GCCAGGGCTG CGGAACGCAG CTCCGCTCTG
    CGTGAGGAGG TCCAGACCCT CCGGGAGGAG GCTGAGAAGC AGCGGGTGGC TTCTGAGAGC
    CTGCGGCAGG AGCTGGCATC GCAGGCGGAG CGAGCAGAGG AGCTGGGCCA AGAGTTAAAG
    GCCTGGCAGG AGAAGTTCTT TCAGAAGGAG CAAGCCCTCT CGGCCCTGCA GCTGGAGCAC
    ACCAGCACAC AGGCCCTGGC GAGCGAGCTG CTGCCCGTCA AGCACCTGTG CCAGCAGCTG
    CAGGCCGAGC AGGCTGCTGC CGAGAAGCGC CACCGGGAGG AGCTGGAGCA GAGCAAGCAG
    GCGGCTGGCG GGCTGCGAGC AGAGCTGCTG CGGGCCCAGC GCGAGCTCGG GGAGTTGCTG
    CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG CGCATGGCCC AGCAGCTGCG AGCGGAGAAG
    GCCAGCTACG CGGAGCAACT GAGCATGCTG AAGAAGGCTC ACGGCCTCTT GGCGGAGGAG
    AACCGCGGGC TGGGCGAGCG GGCCAGCCTC GGCCGGCAGT TTCTGGAAGT GGAGCTGGAC
    CAGGCCCGCG AGAAGTACAG CCAGGAGCTG GCAGCTGTGC GCGCTGACGC TGAGACCCGT
    CTGGCTGAGA TGCAGCGGGA AGTGCAGAGC ACTGCCCGGG AGCTGGAGGT GATGACCGCC
    AAGTACGAGG GTGCCAAGGC CAAGGTCCTG GAGGAGAGGC AGCGTTTCCA GGAGGAGAGG
    CAGAAACTCA CTGCCCAGGT GGAGCAGCTA GAGTTATTTC AGAGAGAGCA GACTAAACAG
    GTGGAAGAAC TGAGCAAGAA GCTAGCTGAC CATGAGCAAG CCAGCAAGGC GCAGCAGCAG
    AAGCTGAAGG CCCAGGGCGG TGAGAGCCAG CAGGAGGTCC AGCGCCTCCA GGCCCAGCTG
    AGTGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCGG CTGAGCACTA CAAGCTACAG
    ATGGAGAAGG CCAAGACTCA TTATGATGCC AAGAAGCAGC AGAACCAAGA GCTGCAGGAG
    CAGCTGCGAG GCCTGGAGCA GCTGCAGACA GAGAACAAGG AGCTGAGGGC AGAAGTGGAT
    CGGCTGGGCC GGGAGCTGCA GCAGGCTGGG CTGAAGACCA AGGAAGCCGA GCAGACCTGC
    CGCCACCTCA CGGCCCAGGT GCGCAGCCTG GAGGCACAGG TTGCCCATGC CGACCAGCAG
    CTTCGGGACC TGGGCAAGTT CCAGGTGGCG ACTGATGCCT TGAAGAGCCG GGAGCCCCAG
    GCTAAGTCTC AGCTGGACTT GAGTATTGAC AGCCTGGATC TGAGCTGCGA GGAGGGGACC
    CCTCTCTCTG TCACCAGCAA GTTGCCTCGC ACCCAGCCAG ACGGCACCAG CATCCCCGGA
    GAGCCAGCCT CCCCCATCTC CCAGCGTCTG CCCCCCAAAG TGGAGTCCCT GGAGAGCCTC
    TACTTCACGC CCATCCCTGC TCGGGGTCAG GCCCCCCTGG AGAGCAGCCT GGACTCCCTG
    GGGGACATCT CCCTGGACTC AAGCCGGAAA ACCCGCTCCG CTCGTAGGCG CACCACGCAG
    ATCATCAACA TCACCATGAC CAAGAAGCTG GACGTGGAGG ATCCAGACAG TGCCAACGCC
    TCCTTCTACA GCACACAGTC AGCCCCCACT TCCCAGGCAG GCCTGAGGGC CGCCTCCTCC
    ACCCAGTCTC TAGCCCGCCT GGGCTCTCCC GACGATGGCA ACTCCACTCT ATTCAGCCTG
    CCTGGCTACC GGCCCACCAC TCGCAGCTCC GCTCGCCGTT CCCAGGCCGG GATGTCCAGT
    GGGGCCCCTC CAGGCAGGAA CAGCTTCTAT GTGGGCACCT GCCAGGACGA GCCCGAACAG
    CTGGATGACT GGAACCGCAT TGCAGAGCTG CAGCAGCGCA ACCGAGTATG CCCACCGCAC
    CTGAAGACCT GCTACCCCCT TGAGTCCAGG CCCTCCCTGA GCCTGCCTGC CATCACAGAT
    GAGGAGATAA AAACCGGCGA CCCCCGGGAG ACCCTGCGCC GAGCCAGCAT GCAGCCAACC
    CAGATAGCTG AGGGCGCTGG CATTACCACA CGGCAGCAGC GCAAGCGGGT TTCCTCAGAG
    ACCCACCAGG GCCCCGGCAC ACCCGAGTCA AAGAAGGCCA CCACATGTTT CCCGCGCCCC
    ATGACCCCCC GGGACCGACA CGAAGGGCGC AGACACAGCA GTACCGAGGC CCAGAAGAAG
    GCAGTTCCAG CTGTTAAACA GGCTGACCGC CGCCAGTCTA TGGCCTTCAG CATCCTCAAC
    ACACCCAAGA AGCTTGGAAA CAGCCTTCTG CGGAAGGCAG CCTCCAAGAA AGCCCCCTCC
    AAAGCCTCAC CCAACCCCCG CAGTGGGACC CGCCGCTCTC CGCGCATCGC CACCACTGCA
    GCCAGCGCTG CCACTGCTGC TGCCAGCGCC GCTGCCACCA CCCCCCGGGC AAAGGGCAAG
    GCGAAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330250.1
    CDS54..6425
    Translation

    Target ORF information:

    RefSeq Version XM_015474764.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474764.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCGC
    CCGCAGTCTC TTGGGACAGC CAGCCGAGAA GACGCTTGTG GATTGCGAAC CCAGTCCGAG
    GCTACTGAAA AGACTGAGCC AGAAGGCTCT GAACTGCAGG CCCTGCAGGC AGAGGTGAGC
    CGGCTGGAAC AGCAGGCCCG TGAGCACCAG GAGAAGGCCT CCAGCCTGGA GCAAAGCCTT
    GAGTCTGAGC GCGCTGCCCA CGCGGAGCAG GCCAGTACTC TGAAGACTCT GCAGGGCCAG
    TTAGAGCAGA AGGCCCAGGA GCTGGGGAGC AGTCAGGACG CCTTAGCCTC AACCCAGAGG
    GAGTTGGCCA CCCTCCGTGC CAAGGCCCAG GAGCACAGCA AGGCCGAGGA CGAGTGGAAG
    GCCCAGGTGG CCCGGGGTCA GCAGGAGGCC GAGAGGAAAA ACAGCCTCAT CAGCAGCTTG
    GAGGAGGAGG TGTCCATCCT GAACCGCCAG GTGCTGGAGA AGGAGGGCGA GAGCAAGGAG
    CTGAAGCGGC TGGTGGTCGC TGAGTCAGAG AAGAGTCAGA AGCTGGAAGA GCGGCTGCGC
    CTGCTCCAGG CGGAGACCGC CAGCAGCAGC GCCAGGGCTG CGGAACGCAG CTCCGCTCTG
    CGTGAGGAGG TCCAGACCCT CCGGGAGGAG GCTGAGAAGC AGCGGGTGGC TTCTGAGAGC
    CTGCGGCAGG AGCTGGCATC GCAGGCGGAG CGAGCAGAGG AGCTGGGCCA AGAGTTAAAG
    GCCTGGCAGG AGAAGTTCTT TCAGAAGGAG CAAGCCCTCT CGGCCCTGCA GCTGGAGCAC
    ACCAGCACAC AGGCCCTGGC GAGCGAGCTG CTGCCCGTCA AGCACCTGTG CCAGCAGCTG
    CAGGCCGAGC AGGCTGCTGC CGAGAAGCGC CACCGGGAGG AGCTGGAGCA GAGCAAGCAG
    GCGGCTGGCG GGCTGCGAGC AGAGCTGCTG CGGGCCCAGC GCGAGCTCGG GGAGTTGCTG
    CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG CGCATGGCCC AGCAGCTGCG AGCGGAGAAG
    GCCAGCTACG CGGAGCAACT GAGCATGCTG AAGAAGGCTC ACGGCCTCTT GGCGGAGGAG
    AACCGCGGGC TGGGCGAGCG GGCCAGCCTC GGCCGGCAGT TTCTGGAAGT GGAGCTGGAC
    CAGGCCCGCG AGAAGTACAG CCAGGAGCTG GCAGCTGTGC GCGCTGACGC TGAGACCCGT
    CTGGCTGAGA TGCAGCGGGA AGTGCAGAGC ACTGCCCGGG AGCTGGAGGT GATGACCGCC
    AAGTACGAGG GTGCCAAGGC CAAGGTCCTG GAGGAGAGGC AGCGTTTCCA GGAGGAGAGG
    CAGAAACTCA CTGCCCAGGT GGAGCAGCTA GAGTTATTTC AGAGAGAGCA GACTAAACAG
    GTGGAAGAAC TGAGCAAGAA GCTAGCTGAC CATGAGCAAG CCAGCAAGGC GCAGCAGCAG
    AAGCTGAAGG CCCAGGGCGG TGAGAGCCAG CAGGAGGTCC AGCGCCTCCA GGCCCAGCTG
    AGTGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCGG CTGAGCACTA CAAGCTACAG
    ATGGAGAAGG CCAAGACTCA TTATGATGCC AAGAAGCAGC AGAACCAAGA GCTGCAGGAG
    CAGCTGCGAG GCCTGGAGCA GCTGCAGACA GAGAACAAGG AGCTGAGGGC AGAAGTGGAT
    CGGCTGGGCC GGGAGCTGCA GCAGGCTGGG CTGAAGACCA AGGAAGCCGA GCAGACCTGC
    CGCCACCTCA CGGCCCAGGT GCGCAGCCTG GAGGCACAGG TTGCCCATGC CGACCAGCAG
    CTTCGGGACC TGGGCAAGTT CCAGGTGGCG ACTGATGCCT TGAAGAGCCG GGAGCCCCAG
    GCTAAGTCTC AGCTGGACTT GAGTATTGAC AGCCTGGATC TGAGCTGCGA GGAGGGGACC
    CCTCTCTCTG TCACCAGCAA GTTGCCTCGC ACCCAGCCAG ACGGCACCAG CATCCCCGGA
    GAGCCAGCCT CCCCCATCTC CCAGCGTCTG CCCCCCAAAG TGGAGTCCCT GGAGAGCCTC
    TACTTCACGC CCATCCCTGC TCGGGGTCAG GCCCCCCTGG AGAGCAGCCT GGACTCCCTG
    GGGGACATCT CCCTGGACTC AAGCCGGAAA ACCCGCTCCG CTCGTAGGCG CACCACGCAG
    ATCATCAACA TCACCATGAC CAAGAAGCTG GACGTGGAGG ATCCAGACAG TGCCAACGCC
    TCCTTCTACA GCACACAGTC AGCCCCCACT TCCCAGGCAG GCCTGAGGGC CGCCTCCTCC
    ACCCAGTCTC TAGCCCGCCT GGGCTCTCCC GACGATGGCA ACTCCACTCT ATTCAGCCTG
    CCTGGCTACC GGCCCACCAC TCGCAGCTCC GCTCGCCGTT CCCAGGCCGG GATGTCCAGT
    GGGGCCCCTC CAGGCAGGAA CAGCTTCTAT GTGGGCACCT GCCAGGACGA GCCCGAACAG
    CTGGATGACT GGAACCGCAT TGCAGAGCTG CAGCAGCGCA ACCGAGTATG CCCACCGCAC
    CTGAAGACCT GCTACCCCCT TGAGTCCAGG CCCTCCCTGA GCCTGCCTGC CATCACAGAT
    GAGGAGATAA AAACCGGCGA CCCCCGGGAG ACCCTGCGCC GAGCCAGCAT GCAGCCAACC
    CAGATAGCTG AGGGCGCTGG CATTACCACA CGGCAGCAGC GCAAGCGGGT TTCCTCAGAG
    ACCCACCAGG GCCCCGGCAC ACCCGAGTCA AAGAAGGCCA CCACATGTTT CCCGCGCCCC
    ATGACCCCCC GGGACCGACA CGAAGGGCGC AGACACAGCA GTACCGAGGC CCAGAAGAAG
    GCAGTTCCAG CTGTTAAACA GGCTGACCGC CGCCAGTCTA TGGCCTTCAG CATCCTCAAC
    ACACCCAAGA AGCTTGGAAA CAGCCTTCTG CGGAAGGCAG CCTCCAAGAA AGCCCCCTCC
    AAAGCCTCAC CCAACCCCCG CAGTGGGACC CGCCGCTCTC CGCGCATCGC CACCACTGCA
    GCCAGCGCTG CCACTGCTGC TGCCAGCGCC GCTGCCACCA CCCCCCGGGC AAAGGGCAAG
    GCGAAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26522
    Clone ID Related Accession (Same CDS sequence) XM_005216174.3 , XM_010812655.2 , XM_005216172.1 , XM_015474764.1 , XM_005216171.3 , XM_015474763.1 , XM_005216173.3
    Accession Version XM_005216171.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear mitotic apparatus protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104415.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216171.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCGC
    CCGCAGTCTC TTGGGACAGC CAGCCGAGAA GACGCTTGTG GATTGCGAAC CCAGTCCGAG
    GCTACTGAAA AGACTGAGCC AGAAGGCTCT GAACTGCAGG CCCTGCAGGC AGAGGTGAGC
    CGGCTGGAAC AGCAGGCCCG TGAGCACCAG GAGAAGGCCT CCAGCCTGGA GCAAAGCCTT
    GAGTCTGAGC GCGCTGCCCA CGCGGAGCAG GCCAGTACTC TGAAGACTCT GCAGGGCCAG
    TTAGAGCAGA AGGCCCAGGA GCTGGGGAGC AGTCAGGACG CCTTAGCCTC AACCCAGAGG
    GAGTTGGCCA CCCTCCGTGC CAAGGCCCAG GAGCACAGCA AGGCCGAGGA CGAGTGGAAG
    GCCCAGGTGG CCCGGGGTCA GCAGGAGGCC GAGAGGAAAA ACAGCCTCAT CAGCAGCTTG
    GAGGAGGAGG TGTCCATCCT GAACCGCCAG GTGCTGGAGA AGGAGGGCGA GAGCAAGGAG
    CTGAAGCGGC TGGTGGTCGC TGAGTCAGAG AAGAGTCAGA AGCTGGAAGA GCGGCTGCGC
    CTGCTCCAGG CGGAGACCGC CAGCAGCAGC GCCAGGGCTG CGGAACGCAG CTCCGCTCTG
    CGTGAGGAGG TCCAGACCCT CCGGGAGGAG GCTGAGAAGC AGCGGGTGGC TTCTGAGAGC
    CTGCGGCAGG AGCTGGCATC GCAGGCGGAG CGAGCAGAGG AGCTGGGCCA AGAGTTAAAG
    GCCTGGCAGG AGAAGTTCTT TCAGAAGGAG CAAGCCCTCT CGGCCCTGCA GCTGGAGCAC
    ACCAGCACAC AGGCCCTGGC GAGCGAGCTG CTGCCCGTCA AGCACCTGTG CCAGCAGCTG
    CAGGCCGAGC AGGCTGCTGC CGAGAAGCGC CACCGGGAGG AGCTGGAGCA GAGCAAGCAG
    GCGGCTGGCG GGCTGCGAGC AGAGCTGCTG CGGGCCCAGC GCGAGCTCGG GGAGTTGCTG
    CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG CGCATGGCCC AGCAGCTGCG AGCGGAGAAG
    GCCAGCTACG CGGAGCAACT GAGCATGCTG AAGAAGGCTC ACGGCCTCTT GGCGGAGGAG
    AACCGCGGGC TGGGCGAGCG GGCCAGCCTC GGCCGGCAGT TTCTGGAAGT GGAGCTGGAC
    CAGGCCCGCG AGAAGTACAG CCAGGAGCTG GCAGCTGTGC GCGCTGACGC TGAGACCCGT
    CTGGCTGAGA TGCAGCGGGA AGTGCAGAGC ACTGCCCGGG AGCTGGAGGT GATGACCGCC
    AAGTACGAGG GTGCCAAGGC CAAGGTCCTG GAGGAGAGGC AGCGTTTCCA GGAGGAGAGG
    CAGAAACTCA CTGCCCAGGT GGAGCAGCTA GAGTTATTTC AGAGAGAGCA GACTAAACAG
    GTGGAAGAAC TGAGCAAGAA GCTAGCTGAC CATGAGCAAG CCAGCAAGGC GCAGCAGCAG
    AAGCTGAAGG CCCAGGGCGG TGAGAGCCAG CAGGAGGTCC AGCGCCTCCA GGCCCAGCTG
    AGTGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCGG CTGAGCACTA CAAGCTACAG
    ATGGAGAAGG CCAAGACTCA TTATGATGCC AAGAAGCAGC AGAACCAAGA GCTGCAGGAG
    CAGCTGCGAG GCCTGGAGCA GCTGCAGACA GAGAACAAGG AGCTGAGGGC AGAAGTGGAT
    CGGCTGGGCC GGGAGCTGCA GCAGGCTGGG CTGAAGACCA AGGAAGCCGA GCAGACCTGC
    CGCCACCTCA CGGCCCAGGT GCGCAGCCTG GAGGCACAGG TTGCCCATGC CGACCAGCAG
    CTTCGGGACC TGGGCAAGTT CCAGGTGGCG ACTGATGCCT TGAAGAGCCG GGAGCCCCAG
    GCTAAGTCTC AGCTGGACTT GAGTATTGAC AGCCTGGATC TGAGCTGCGA GGAGGGGACC
    CCTCTCTCTG TCACCAGCAA GTTGCCTCGC ACCCAGCCAG ACGGCACCAG CATCCCCGGA
    GAGCCAGCCT CCCCCATCTC CCAGCGTCTG CCCCCCAAAG TGGAGTCCCT GGAGAGCCTC
    TACTTCACGC CCATCCCTGC TCGGGGTCAG GCCCCCCTGG AGAGCAGCCT GGACTCCCTG
    GGGGACATCT CCCTGGACTC AAGCCGGAAA ACCCGCTCCG CTCGTAGGCG CACCACGCAG
    ATCATCAACA TCACCATGAC CAAGAAGCTG GACGTGGAGG ATCCAGACAG TGCCAACGCC
    TCCTTCTACA GCACACAGTC AGCCCCCACT TCCCAGGCAG GCCTGAGGGC CGCCTCCTCC
    ACCCAGTCTC TAGCCCGCCT GGGCTCTCCC GACGATGGCA ACTCCACTCT ATTCAGCCTG
    CCTGGCTACC GGCCCACCAC TCGCAGCTCC GCTCGCCGTT CCCAGGCCGG GATGTCCAGT
    GGGGCCCCTC CAGGCAGGAA CAGCTTCTAT GTGGGCACCT GCCAGGACGA GCCCGAACAG
    CTGGATGACT GGAACCGCAT TGCAGAGCTG CAGCAGCGCA ACCGAGTATG CCCACCGCAC
    CTGAAGACCT GCTACCCCCT TGAGTCCAGG CCCTCCCTGA GCCTGCCTGC CATCACAGAT
    GAGGAGATAA AAACCGGCGA CCCCCGGGAG ACCCTGCGCC GAGCCAGCAT GCAGCCAACC
    CAGATAGCTG AGGGCGCTGG CATTACCACA CGGCAGCAGC GCAAGCGGGT TTCCTCAGAG
    ACCCACCAGG GCCCCGGCAC ACCCGAGTCA AAGAAGGCCA CCACATGTTT CCCGCGCCCC
    ATGACCCCCC GGGACCGACA CGAAGGGCGC AGACACAGCA GTACCGAGGC CCAGAAGAAG
    GCAGTTCCAG CTGTTAAACA GGCTGACCGC CGCCAGTCTA TGGCCTTCAG CATCCTCAAC
    ACACCCAAGA AGCTTGGAAA CAGCCTTCTG CGGAAGGCAG CCTCCAAGAA AGCCCCCTCC
    AAAGCCTCAC CCAACCCCCG CAGTGGGACC CGCCGCTCTC CGCGCATCGC CACCACTGCA
    GCCAGCGCTG CCACTGCTGC TGCCAGCGCC GCTGCCACCA CCCCCCGGGC AAAGGGCAAG
    GCGAAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216228.1
    CDS239..6610
    Translation

    Target ORF information:

    RefSeq Version XM_005216171.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216171.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCGC
    CCGCAGTCTC TTGGGACAGC CAGCCGAGAA GACGCTTGTG GATTGCGAAC CCAGTCCGAG
    GCTACTGAAA AGACTGAGCC AGAAGGCTCT GAACTGCAGG CCCTGCAGGC AGAGGTGAGC
    CGGCTGGAAC AGCAGGCCCG TGAGCACCAG GAGAAGGCCT CCAGCCTGGA GCAAAGCCTT
    GAGTCTGAGC GCGCTGCCCA CGCGGAGCAG GCCAGTACTC TGAAGACTCT GCAGGGCCAG
    TTAGAGCAGA AGGCCCAGGA GCTGGGGAGC AGTCAGGACG CCTTAGCCTC AACCCAGAGG
    GAGTTGGCCA CCCTCCGTGC CAAGGCCCAG GAGCACAGCA AGGCCGAGGA CGAGTGGAAG
    GCCCAGGTGG CCCGGGGTCA GCAGGAGGCC GAGAGGAAAA ACAGCCTCAT CAGCAGCTTG
    GAGGAGGAGG TGTCCATCCT GAACCGCCAG GTGCTGGAGA AGGAGGGCGA GAGCAAGGAG
    CTGAAGCGGC TGGTGGTCGC TGAGTCAGAG AAGAGTCAGA AGCTGGAAGA GCGGCTGCGC
    CTGCTCCAGG CGGAGACCGC CAGCAGCAGC GCCAGGGCTG CGGAACGCAG CTCCGCTCTG
    CGTGAGGAGG TCCAGACCCT CCGGGAGGAG GCTGAGAAGC AGCGGGTGGC TTCTGAGAGC
    CTGCGGCAGG AGCTGGCATC GCAGGCGGAG CGAGCAGAGG AGCTGGGCCA AGAGTTAAAG
    GCCTGGCAGG AGAAGTTCTT TCAGAAGGAG CAAGCCCTCT CGGCCCTGCA GCTGGAGCAC
    ACCAGCACAC AGGCCCTGGC GAGCGAGCTG CTGCCCGTCA AGCACCTGTG CCAGCAGCTG
    CAGGCCGAGC AGGCTGCTGC CGAGAAGCGC CACCGGGAGG AGCTGGAGCA GAGCAAGCAG
    GCGGCTGGCG GGCTGCGAGC AGAGCTGCTG CGGGCCCAGC GCGAGCTCGG GGAGTTGCTG
    CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG CGCATGGCCC AGCAGCTGCG AGCGGAGAAG
    GCCAGCTACG CGGAGCAACT GAGCATGCTG AAGAAGGCTC ACGGCCTCTT GGCGGAGGAG
    AACCGCGGGC TGGGCGAGCG GGCCAGCCTC GGCCGGCAGT TTCTGGAAGT GGAGCTGGAC
    CAGGCCCGCG AGAAGTACAG CCAGGAGCTG GCAGCTGTGC GCGCTGACGC TGAGACCCGT
    CTGGCTGAGA TGCAGCGGGA AGTGCAGAGC ACTGCCCGGG AGCTGGAGGT GATGACCGCC
    AAGTACGAGG GTGCCAAGGC CAAGGTCCTG GAGGAGAGGC AGCGTTTCCA GGAGGAGAGG
    CAGAAACTCA CTGCCCAGGT GGAGCAGCTA GAGTTATTTC AGAGAGAGCA GACTAAACAG
    GTGGAAGAAC TGAGCAAGAA GCTAGCTGAC CATGAGCAAG CCAGCAAGGC GCAGCAGCAG
    AAGCTGAAGG CCCAGGGCGG TGAGAGCCAG CAGGAGGTCC AGCGCCTCCA GGCCCAGCTG
    AGTGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCGG CTGAGCACTA CAAGCTACAG
    ATGGAGAAGG CCAAGACTCA TTATGATGCC AAGAAGCAGC AGAACCAAGA GCTGCAGGAG
    CAGCTGCGAG GCCTGGAGCA GCTGCAGACA GAGAACAAGG AGCTGAGGGC AGAAGTGGAT
    CGGCTGGGCC GGGAGCTGCA GCAGGCTGGG CTGAAGACCA AGGAAGCCGA GCAGACCTGC
    CGCCACCTCA CGGCCCAGGT GCGCAGCCTG GAGGCACAGG TTGCCCATGC CGACCAGCAG
    CTTCGGGACC TGGGCAAGTT CCAGGTGGCG ACTGATGCCT TGAAGAGCCG GGAGCCCCAG
    GCTAAGTCTC AGCTGGACTT GAGTATTGAC AGCCTGGATC TGAGCTGCGA GGAGGGGACC
    CCTCTCTCTG TCACCAGCAA GTTGCCTCGC ACCCAGCCAG ACGGCACCAG CATCCCCGGA
    GAGCCAGCCT CCCCCATCTC CCAGCGTCTG CCCCCCAAAG TGGAGTCCCT GGAGAGCCTC
    TACTTCACGC CCATCCCTGC TCGGGGTCAG GCCCCCCTGG AGAGCAGCCT GGACTCCCTG
    GGGGACATCT CCCTGGACTC AAGCCGGAAA ACCCGCTCCG CTCGTAGGCG CACCACGCAG
    ATCATCAACA TCACCATGAC CAAGAAGCTG GACGTGGAGG ATCCAGACAG TGCCAACGCC
    TCCTTCTACA GCACACAGTC AGCCCCCACT TCCCAGGCAG GCCTGAGGGC CGCCTCCTCC
    ACCCAGTCTC TAGCCCGCCT GGGCTCTCCC GACGATGGCA ACTCCACTCT ATTCAGCCTG
    CCTGGCTACC GGCCCACCAC TCGCAGCTCC GCTCGCCGTT CCCAGGCCGG GATGTCCAGT
    GGGGCCCCTC CAGGCAGGAA CAGCTTCTAT GTGGGCACCT GCCAGGACGA GCCCGAACAG
    CTGGATGACT GGAACCGCAT TGCAGAGCTG CAGCAGCGCA ACCGAGTATG CCCACCGCAC
    CTGAAGACCT GCTACCCCCT TGAGTCCAGG CCCTCCCTGA GCCTGCCTGC CATCACAGAT
    GAGGAGATAA AAACCGGCGA CCCCCGGGAG ACCCTGCGCC GAGCCAGCAT GCAGCCAACC
    CAGATAGCTG AGGGCGCTGG CATTACCACA CGGCAGCAGC GCAAGCGGGT TTCCTCAGAG
    ACCCACCAGG GCCCCGGCAC ACCCGAGTCA AAGAAGGCCA CCACATGTTT CCCGCGCCCC
    ATGACCCCCC GGGACCGACA CGAAGGGCGC AGACACAGCA GTACCGAGGC CCAGAAGAAG
    GCAGTTCCAG CTGTTAAACA GGCTGACCGC CGCCAGTCTA TGGCCTTCAG CATCCTCAAC
    ACACCCAAGA AGCTTGGAAA CAGCCTTCTG CGGAAGGCAG CCTCCAAGAA AGCCCCCTCC
    AAAGCCTCAC CCAACCCCCG CAGTGGGACC CGCCGCTCTC CGCGCATCGC CACCACTGCA
    GCCAGCGCTG CCACTGCTGC TGCCAGCGCC GCTGCCACCA CCCCCCGGGC AAAGGGCAAG
    GCGAAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26522
    Clone ID Related Accession (Same CDS sequence) XM_005216174.3 , XM_010812655.2 , XM_005216172.1 , XM_015474764.1 , XM_005216171.3 , XM_015474763.1 , XM_005216173.3
    Accession Version XM_015474763.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear mitotic apparatus protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104415.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCGC
    CCGCAGTCTC TTGGGACAGC CAGCCGAGAA GACGCTTGTG GATTGCGAAC CCAGTCCGAG
    GCTACTGAAA AGACTGAGCC AGAAGGCTCT GAACTGCAGG CCCTGCAGGC AGAGGTGAGC
    CGGCTGGAAC AGCAGGCCCG TGAGCACCAG GAGAAGGCCT CCAGCCTGGA GCAAAGCCTT
    GAGTCTGAGC GCGCTGCCCA CGCGGAGCAG GCCAGTACTC TGAAGACTCT GCAGGGCCAG
    TTAGAGCAGA AGGCCCAGGA GCTGGGGAGC AGTCAGGACG CCTTAGCCTC AACCCAGAGG
    GAGTTGGCCA CCCTCCGTGC CAAGGCCCAG GAGCACAGCA AGGCCGAGGA CGAGTGGAAG
    GCCCAGGTGG CCCGGGGTCA GCAGGAGGCC GAGAGGAAAA ACAGCCTCAT CAGCAGCTTG
    GAGGAGGAGG TGTCCATCCT GAACCGCCAG GTGCTGGAGA AGGAGGGCGA GAGCAAGGAG
    CTGAAGCGGC TGGTGGTCGC TGAGTCAGAG AAGAGTCAGA AGCTGGAAGA GCGGCTGCGC
    CTGCTCCAGG CGGAGACCGC CAGCAGCAGC GCCAGGGCTG CGGAACGCAG CTCCGCTCTG
    CGTGAGGAGG TCCAGACCCT CCGGGAGGAG GCTGAGAAGC AGCGGGTGGC TTCTGAGAGC
    CTGCGGCAGG AGCTGGCATC GCAGGCGGAG CGAGCAGAGG AGCTGGGCCA AGAGTTAAAG
    GCCTGGCAGG AGAAGTTCTT TCAGAAGGAG CAAGCCCTCT CGGCCCTGCA GCTGGAGCAC
    ACCAGCACAC AGGCCCTGGC GAGCGAGCTG CTGCCCGTCA AGCACCTGTG CCAGCAGCTG
    CAGGCCGAGC AGGCTGCTGC CGAGAAGCGC CACCGGGAGG AGCTGGAGCA GAGCAAGCAG
    GCGGCTGGCG GGCTGCGAGC AGAGCTGCTG CGGGCCCAGC GCGAGCTCGG GGAGTTGCTG
    CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG CGCATGGCCC AGCAGCTGCG AGCGGAGAAG
    GCCAGCTACG CGGAGCAACT GAGCATGCTG AAGAAGGCTC ACGGCCTCTT GGCGGAGGAG
    AACCGCGGGC TGGGCGAGCG GGCCAGCCTC GGCCGGCAGT TTCTGGAAGT GGAGCTGGAC
    CAGGCCCGCG AGAAGTACAG CCAGGAGCTG GCAGCTGTGC GCGCTGACGC TGAGACCCGT
    CTGGCTGAGA TGCAGCGGGA AGTGCAGAGC ACTGCCCGGG AGCTGGAGGT GATGACCGCC
    AAGTACGAGG GTGCCAAGGC CAAGGTCCTG GAGGAGAGGC AGCGTTTCCA GGAGGAGAGG
    CAGAAACTCA CTGCCCAGGT GGAGCAGCTA GAGTTATTTC AGAGAGAGCA GACTAAACAG
    GTGGAAGAAC TGAGCAAGAA GCTAGCTGAC CATGAGCAAG CCAGCAAGGC GCAGCAGCAG
    AAGCTGAAGG CCCAGGGCGG TGAGAGCCAG CAGGAGGTCC AGCGCCTCCA GGCCCAGCTG
    AGTGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCGG CTGAGCACTA CAAGCTACAG
    ATGGAGAAGG CCAAGACTCA TTATGATGCC AAGAAGCAGC AGAACCAAGA GCTGCAGGAG
    CAGCTGCGAG GCCTGGAGCA GCTGCAGACA GAGAACAAGG AGCTGAGGGC AGAAGTGGAT
    CGGCTGGGCC GGGAGCTGCA GCAGGCTGGG CTGAAGACCA AGGAAGCCGA GCAGACCTGC
    CGCCACCTCA CGGCCCAGGT GCGCAGCCTG GAGGCACAGG TTGCCCATGC CGACCAGCAG
    CTTCGGGACC TGGGCAAGTT CCAGGTGGCG ACTGATGCCT TGAAGAGCCG GGAGCCCCAG
    GCTAAGTCTC AGCTGGACTT GAGTATTGAC AGCCTGGATC TGAGCTGCGA GGAGGGGACC
    CCTCTCTCTG TCACCAGCAA GTTGCCTCGC ACCCAGCCAG ACGGCACCAG CATCCCCGGA
    GAGCCAGCCT CCCCCATCTC CCAGCGTCTG CCCCCCAAAG TGGAGTCCCT GGAGAGCCTC
    TACTTCACGC CCATCCCTGC TCGGGGTCAG GCCCCCCTGG AGAGCAGCCT GGACTCCCTG
    GGGGACATCT CCCTGGACTC AAGCCGGAAA ACCCGCTCCG CTCGTAGGCG CACCACGCAG
    ATCATCAACA TCACCATGAC CAAGAAGCTG GACGTGGAGG ATCCAGACAG TGCCAACGCC
    TCCTTCTACA GCACACAGTC AGCCCCCACT TCCCAGGCAG GCCTGAGGGC CGCCTCCTCC
    ACCCAGTCTC TAGCCCGCCT GGGCTCTCCC GACGATGGCA ACTCCACTCT ATTCAGCCTG
    CCTGGCTACC GGCCCACCAC TCGCAGCTCC GCTCGCCGTT CCCAGGCCGG GATGTCCAGT
    GGGGCCCCTC CAGGCAGGAA CAGCTTCTAT GTGGGCACCT GCCAGGACGA GCCCGAACAG
    CTGGATGACT GGAACCGCAT TGCAGAGCTG CAGCAGCGCA ACCGAGTATG CCCACCGCAC
    CTGAAGACCT GCTACCCCCT TGAGTCCAGG CCCTCCCTGA GCCTGCCTGC CATCACAGAT
    GAGGAGATAA AAACCGGCGA CCCCCGGGAG ACCCTGCGCC GAGCCAGCAT GCAGCCAACC
    CAGATAGCTG AGGGCGCTGG CATTACCACA CGGCAGCAGC GCAAGCGGGT TTCCTCAGAG
    ACCCACCAGG GCCCCGGCAC ACCCGAGTCA AAGAAGGCCA CCACATGTTT CCCGCGCCCC
    ATGACCCCCC GGGACCGACA CGAAGGGCGC AGACACAGCA GTACCGAGGC CCAGAAGAAG
    GCAGTTCCAG CTGTTAAACA GGCTGACCGC CGCCAGTCTA TGGCCTTCAG CATCCTCAAC
    ACACCCAAGA AGCTTGGAAA CAGCCTTCTG CGGAAGGCAG CCTCCAAGAA AGCCCCCTCC
    AAAGCCTCAC CCAACCCCCG CAGTGGGACC CGCCGCTCTC CGCGCATCGC CACCACTGCA
    GCCAGCGCTG CCACTGCTGC TGCCAGCGCC GCTGCCACCA CCCCCCGGGC AAAGGGCAAG
    GCGAAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330249.1
    CDS233..6604
    Translation

    Target ORF information:

    RefSeq Version XM_015474763.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474763.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCGC
    CCGCAGTCTC TTGGGACAGC CAGCCGAGAA GACGCTTGTG GATTGCGAAC CCAGTCCGAG
    GCTACTGAAA AGACTGAGCC AGAAGGCTCT GAACTGCAGG CCCTGCAGGC AGAGGTGAGC
    CGGCTGGAAC AGCAGGCCCG TGAGCACCAG GAGAAGGCCT CCAGCCTGGA GCAAAGCCTT
    GAGTCTGAGC GCGCTGCCCA CGCGGAGCAG GCCAGTACTC TGAAGACTCT GCAGGGCCAG
    TTAGAGCAGA AGGCCCAGGA GCTGGGGAGC AGTCAGGACG CCTTAGCCTC AACCCAGAGG
    GAGTTGGCCA CCCTCCGTGC CAAGGCCCAG GAGCACAGCA AGGCCGAGGA CGAGTGGAAG
    GCCCAGGTGG CCCGGGGTCA GCAGGAGGCC GAGAGGAAAA ACAGCCTCAT CAGCAGCTTG
    GAGGAGGAGG TGTCCATCCT GAACCGCCAG GTGCTGGAGA AGGAGGGCGA GAGCAAGGAG
    CTGAAGCGGC TGGTGGTCGC TGAGTCAGAG AAGAGTCAGA AGCTGGAAGA GCGGCTGCGC
    CTGCTCCAGG CGGAGACCGC CAGCAGCAGC GCCAGGGCTG CGGAACGCAG CTCCGCTCTG
    CGTGAGGAGG TCCAGACCCT CCGGGAGGAG GCTGAGAAGC AGCGGGTGGC TTCTGAGAGC
    CTGCGGCAGG AGCTGGCATC GCAGGCGGAG CGAGCAGAGG AGCTGGGCCA AGAGTTAAAG
    GCCTGGCAGG AGAAGTTCTT TCAGAAGGAG CAAGCCCTCT CGGCCCTGCA GCTGGAGCAC
    ACCAGCACAC AGGCCCTGGC GAGCGAGCTG CTGCCCGTCA AGCACCTGTG CCAGCAGCTG
    CAGGCCGAGC AGGCTGCTGC CGAGAAGCGC CACCGGGAGG AGCTGGAGCA GAGCAAGCAG
    GCGGCTGGCG GGCTGCGAGC AGAGCTGCTG CGGGCCCAGC GCGAGCTCGG GGAGTTGCTG
    CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG CGCATGGCCC AGCAGCTGCG AGCGGAGAAG
    GCCAGCTACG CGGAGCAACT GAGCATGCTG AAGAAGGCTC ACGGCCTCTT GGCGGAGGAG
    AACCGCGGGC TGGGCGAGCG GGCCAGCCTC GGCCGGCAGT TTCTGGAAGT GGAGCTGGAC
    CAGGCCCGCG AGAAGTACAG CCAGGAGCTG GCAGCTGTGC GCGCTGACGC TGAGACCCGT
    CTGGCTGAGA TGCAGCGGGA AGTGCAGAGC ACTGCCCGGG AGCTGGAGGT GATGACCGCC
    AAGTACGAGG GTGCCAAGGC CAAGGTCCTG GAGGAGAGGC AGCGTTTCCA GGAGGAGAGG
    CAGAAACTCA CTGCCCAGGT GGAGCAGCTA GAGTTATTTC AGAGAGAGCA GACTAAACAG
    GTGGAAGAAC TGAGCAAGAA GCTAGCTGAC CATGAGCAAG CCAGCAAGGC GCAGCAGCAG
    AAGCTGAAGG CCCAGGGCGG TGAGAGCCAG CAGGAGGTCC AGCGCCTCCA GGCCCAGCTG
    AGTGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCGG CTGAGCACTA CAAGCTACAG
    ATGGAGAAGG CCAAGACTCA TTATGATGCC AAGAAGCAGC AGAACCAAGA GCTGCAGGAG
    CAGCTGCGAG GCCTGGAGCA GCTGCAGACA GAGAACAAGG AGCTGAGGGC AGAAGTGGAT
    CGGCTGGGCC GGGAGCTGCA GCAGGCTGGG CTGAAGACCA AGGAAGCCGA GCAGACCTGC
    CGCCACCTCA CGGCCCAGGT GCGCAGCCTG GAGGCACAGG TTGCCCATGC CGACCAGCAG
    CTTCGGGACC TGGGCAAGTT CCAGGTGGCG ACTGATGCCT TGAAGAGCCG GGAGCCCCAG
    GCTAAGTCTC AGCTGGACTT GAGTATTGAC AGCCTGGATC TGAGCTGCGA GGAGGGGACC
    CCTCTCTCTG TCACCAGCAA GTTGCCTCGC ACCCAGCCAG ACGGCACCAG CATCCCCGGA
    GAGCCAGCCT CCCCCATCTC CCAGCGTCTG CCCCCCAAAG TGGAGTCCCT GGAGAGCCTC
    TACTTCACGC CCATCCCTGC TCGGGGTCAG GCCCCCCTGG AGAGCAGCCT GGACTCCCTG
    GGGGACATCT CCCTGGACTC AAGCCGGAAA ACCCGCTCCG CTCGTAGGCG CACCACGCAG
    ATCATCAACA TCACCATGAC CAAGAAGCTG GACGTGGAGG ATCCAGACAG TGCCAACGCC
    TCCTTCTACA GCACACAGTC AGCCCCCACT TCCCAGGCAG GCCTGAGGGC CGCCTCCTCC
    ACCCAGTCTC TAGCCCGCCT GGGCTCTCCC GACGATGGCA ACTCCACTCT ATTCAGCCTG
    CCTGGCTACC GGCCCACCAC TCGCAGCTCC GCTCGCCGTT CCCAGGCCGG GATGTCCAGT
    GGGGCCCCTC CAGGCAGGAA CAGCTTCTAT GTGGGCACCT GCCAGGACGA GCCCGAACAG
    CTGGATGACT GGAACCGCAT TGCAGAGCTG CAGCAGCGCA ACCGAGTATG CCCACCGCAC
    CTGAAGACCT GCTACCCCCT TGAGTCCAGG CCCTCCCTGA GCCTGCCTGC CATCACAGAT
    GAGGAGATAA AAACCGGCGA CCCCCGGGAG ACCCTGCGCC GAGCCAGCAT GCAGCCAACC
    CAGATAGCTG AGGGCGCTGG CATTACCACA CGGCAGCAGC GCAAGCGGGT TTCCTCAGAG
    ACCCACCAGG GCCCCGGCAC ACCCGAGTCA AAGAAGGCCA CCACATGTTT CCCGCGCCCC
    ATGACCCCCC GGGACCGACA CGAAGGGCGC AGACACAGCA GTACCGAGGC CCAGAAGAAG
    GCAGTTCCAG CTGTTAAACA GGCTGACCGC CGCCAGTCTA TGGCCTTCAG CATCCTCAAC
    ACACCCAAGA AGCTTGGAAA CAGCCTTCTG CGGAAGGCAG CCTCCAAGAA AGCCCCCTCC
    AAAGCCTCAC CCAACCCCCG CAGTGGGACC CGCCGCTCTC CGCGCATCGC CACCACTGCA
    GCCAGCGCTG CCACTGCTGC TGCCAGCGCC GCTGCCACCA CCCCCCGGGC AAAGGGCAAG
    GCGAAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26522
    Clone ID Related Accession (Same CDS sequence) XM_005216174.3 , XM_010812655.2 , XM_005216172.1 , XM_015474764.1 , XM_005216171.3 , XM_015474763.1 , XM_005216173.3
    Accession Version XM_005216173.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear mitotic apparatus protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104415.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216173.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCGC
    CCGCAGTCTC TTGGGACAGC CAGCCGAGAA GACGCTTGTG GATTGCGAAC CCAGTCCGAG
    GCTACTGAAA AGACTGAGCC AGAAGGCTCT GAACTGCAGG CCCTGCAGGC AGAGGTGAGC
    CGGCTGGAAC AGCAGGCCCG TGAGCACCAG GAGAAGGCCT CCAGCCTGGA GCAAAGCCTT
    GAGTCTGAGC GCGCTGCCCA CGCGGAGCAG GCCAGTACTC TGAAGACTCT GCAGGGCCAG
    TTAGAGCAGA AGGCCCAGGA GCTGGGGAGC AGTCAGGACG CCTTAGCCTC AACCCAGAGG
    GAGTTGGCCA CCCTCCGTGC CAAGGCCCAG GAGCACAGCA AGGCCGAGGA CGAGTGGAAG
    GCCCAGGTGG CCCGGGGTCA GCAGGAGGCC GAGAGGAAAA ACAGCCTCAT CAGCAGCTTG
    GAGGAGGAGG TGTCCATCCT GAACCGCCAG GTGCTGGAGA AGGAGGGCGA GAGCAAGGAG
    CTGAAGCGGC TGGTGGTCGC TGAGTCAGAG AAGAGTCAGA AGCTGGAAGA GCGGCTGCGC
    CTGCTCCAGG CGGAGACCGC CAGCAGCAGC GCCAGGGCTG CGGAACGCAG CTCCGCTCTG
    CGTGAGGAGG TCCAGACCCT CCGGGAGGAG GCTGAGAAGC AGCGGGTGGC TTCTGAGAGC
    CTGCGGCAGG AGCTGGCATC GCAGGCGGAG CGAGCAGAGG AGCTGGGCCA AGAGTTAAAG
    GCCTGGCAGG AGAAGTTCTT TCAGAAGGAG CAAGCCCTCT CGGCCCTGCA GCTGGAGCAC
    ACCAGCACAC AGGCCCTGGC GAGCGAGCTG CTGCCCGTCA AGCACCTGTG CCAGCAGCTG
    CAGGCCGAGC AGGCTGCTGC CGAGAAGCGC CACCGGGAGG AGCTGGAGCA GAGCAAGCAG
    GCGGCTGGCG GGCTGCGAGC AGAGCTGCTG CGGGCCCAGC GCGAGCTCGG GGAGTTGCTG
    CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG CGCATGGCCC AGCAGCTGCG AGCGGAGAAG
    GCCAGCTACG CGGAGCAACT GAGCATGCTG AAGAAGGCTC ACGGCCTCTT GGCGGAGGAG
    AACCGCGGGC TGGGCGAGCG GGCCAGCCTC GGCCGGCAGT TTCTGGAAGT GGAGCTGGAC
    CAGGCCCGCG AGAAGTACAG CCAGGAGCTG GCAGCTGTGC GCGCTGACGC TGAGACCCGT
    CTGGCTGAGA TGCAGCGGGA AGTGCAGAGC ACTGCCCGGG AGCTGGAGGT GATGACCGCC
    AAGTACGAGG GTGCCAAGGC CAAGGTCCTG GAGGAGAGGC AGCGTTTCCA GGAGGAGAGG
    CAGAAACTCA CTGCCCAGGT GGAGCAGCTA GAGTTATTTC AGAGAGAGCA GACTAAACAG
    GTGGAAGAAC TGAGCAAGAA GCTAGCTGAC CATGAGCAAG CCAGCAAGGC GCAGCAGCAG
    AAGCTGAAGG CCCAGGGCGG TGAGAGCCAG CAGGAGGTCC AGCGCCTCCA GGCCCAGCTG
    AGTGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCGG CTGAGCACTA CAAGCTACAG
    ATGGAGAAGG CCAAGACTCA TTATGATGCC AAGAAGCAGC AGAACCAAGA GCTGCAGGAG
    CAGCTGCGAG GCCTGGAGCA GCTGCAGACA GAGAACAAGG AGCTGAGGGC AGAAGTGGAT
    CGGCTGGGCC GGGAGCTGCA GCAGGCTGGG CTGAAGACCA AGGAAGCCGA GCAGACCTGC
    CGCCACCTCA CGGCCCAGGT GCGCAGCCTG GAGGCACAGG TTGCCCATGC CGACCAGCAG
    CTTCGGGACC TGGGCAAGTT CCAGGTGGCG ACTGATGCCT TGAAGAGCCG GGAGCCCCAG
    GCTAAGTCTC AGCTGGACTT GAGTATTGAC AGCCTGGATC TGAGCTGCGA GGAGGGGACC
    CCTCTCTCTG TCACCAGCAA GTTGCCTCGC ACCCAGCCAG ACGGCACCAG CATCCCCGGA
    GAGCCAGCCT CCCCCATCTC CCAGCGTCTG CCCCCCAAAG TGGAGTCCCT GGAGAGCCTC
    TACTTCACGC CCATCCCTGC TCGGGGTCAG GCCCCCCTGG AGAGCAGCCT GGACTCCCTG
    GGGGACATCT CCCTGGACTC AAGCCGGAAA ACCCGCTCCG CTCGTAGGCG CACCACGCAG
    ATCATCAACA TCACCATGAC CAAGAAGCTG GACGTGGAGG ATCCAGACAG TGCCAACGCC
    TCCTTCTACA GCACACAGTC AGCCCCCACT TCCCAGGCAG GCCTGAGGGC CGCCTCCTCC
    ACCCAGTCTC TAGCCCGCCT GGGCTCTCCC GACGATGGCA ACTCCACTCT ATTCAGCCTG
    CCTGGCTACC GGCCCACCAC TCGCAGCTCC GCTCGCCGTT CCCAGGCCGG GATGTCCAGT
    GGGGCCCCTC CAGGCAGGAA CAGCTTCTAT GTGGGCACCT GCCAGGACGA GCCCGAACAG
    CTGGATGACT GGAACCGCAT TGCAGAGCTG CAGCAGCGCA ACCGAGTATG CCCACCGCAC
    CTGAAGACCT GCTACCCCCT TGAGTCCAGG CCCTCCCTGA GCCTGCCTGC CATCACAGAT
    GAGGAGATAA AAACCGGCGA CCCCCGGGAG ACCCTGCGCC GAGCCAGCAT GCAGCCAACC
    CAGATAGCTG AGGGCGCTGG CATTACCACA CGGCAGCAGC GCAAGCGGGT TTCCTCAGAG
    ACCCACCAGG GCCCCGGCAC ACCCGAGTCA AAGAAGGCCA CCACATGTTT CCCGCGCCCC
    ATGACCCCCC GGGACCGACA CGAAGGGCGC AGACACAGCA GTACCGAGGC CCAGAAGAAG
    GCAGTTCCAG CTGTTAAACA GGCTGACCGC CGCCAGTCTA TGGCCTTCAG CATCCTCAAC
    ACACCCAAGA AGCTTGGAAA CAGCCTTCTG CGGAAGGCAG CCTCCAAGAA AGCCCCCTCC
    AAAGCCTCAC CCAACCCCCG CAGTGGGACC CGCCGCTCTC CGCGCATCGC CACCACTGCA
    GCCAGCGCTG CCACTGCTGC TGCCAGCGCC GCTGCCACCA CCCCCCGGGC AAAGGGCAAG
    GCGAAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216230.1
    CDS132..6503
    Translation

    Target ORF information:

    RefSeq Version XM_005216173.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216173.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCGC
    CCGCAGTCTC TTGGGACAGC CAGCCGAGAA GACGCTTGTG GATTGCGAAC CCAGTCCGAG
    GCTACTGAAA AGACTGAGCC AGAAGGCTCT GAACTGCAGG CCCTGCAGGC AGAGGTGAGC
    CGGCTGGAAC AGCAGGCCCG TGAGCACCAG GAGAAGGCCT CCAGCCTGGA GCAAAGCCTT
    GAGTCTGAGC GCGCTGCCCA CGCGGAGCAG GCCAGTACTC TGAAGACTCT GCAGGGCCAG
    TTAGAGCAGA AGGCCCAGGA GCTGGGGAGC AGTCAGGACG CCTTAGCCTC AACCCAGAGG
    GAGTTGGCCA CCCTCCGTGC CAAGGCCCAG GAGCACAGCA AGGCCGAGGA CGAGTGGAAG
    GCCCAGGTGG CCCGGGGTCA GCAGGAGGCC GAGAGGAAAA ACAGCCTCAT CAGCAGCTTG
    GAGGAGGAGG TGTCCATCCT GAACCGCCAG GTGCTGGAGA AGGAGGGCGA GAGCAAGGAG
    CTGAAGCGGC TGGTGGTCGC TGAGTCAGAG AAGAGTCAGA AGCTGGAAGA GCGGCTGCGC
    CTGCTCCAGG CGGAGACCGC CAGCAGCAGC GCCAGGGCTG CGGAACGCAG CTCCGCTCTG
    CGTGAGGAGG TCCAGACCCT CCGGGAGGAG GCTGAGAAGC AGCGGGTGGC TTCTGAGAGC
    CTGCGGCAGG AGCTGGCATC GCAGGCGGAG CGAGCAGAGG AGCTGGGCCA AGAGTTAAAG
    GCCTGGCAGG AGAAGTTCTT TCAGAAGGAG CAAGCCCTCT CGGCCCTGCA GCTGGAGCAC
    ACCAGCACAC AGGCCCTGGC GAGCGAGCTG CTGCCCGTCA AGCACCTGTG CCAGCAGCTG
    CAGGCCGAGC AGGCTGCTGC CGAGAAGCGC CACCGGGAGG AGCTGGAGCA GAGCAAGCAG
    GCGGCTGGCG GGCTGCGAGC AGAGCTGCTG CGGGCCCAGC GCGAGCTCGG GGAGTTGCTG
    CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG CGCATGGCCC AGCAGCTGCG AGCGGAGAAG
    GCCAGCTACG CGGAGCAACT GAGCATGCTG AAGAAGGCTC ACGGCCTCTT GGCGGAGGAG
    AACCGCGGGC TGGGCGAGCG GGCCAGCCTC GGCCGGCAGT TTCTGGAAGT GGAGCTGGAC
    CAGGCCCGCG AGAAGTACAG CCAGGAGCTG GCAGCTGTGC GCGCTGACGC TGAGACCCGT
    CTGGCTGAGA TGCAGCGGGA AGTGCAGAGC ACTGCCCGGG AGCTGGAGGT GATGACCGCC
    AAGTACGAGG GTGCCAAGGC CAAGGTCCTG GAGGAGAGGC AGCGTTTCCA GGAGGAGAGG
    CAGAAACTCA CTGCCCAGGT GGAGCAGCTA GAGTTATTTC AGAGAGAGCA GACTAAACAG
    GTGGAAGAAC TGAGCAAGAA GCTAGCTGAC CATGAGCAAG CCAGCAAGGC GCAGCAGCAG
    AAGCTGAAGG CCCAGGGCGG TGAGAGCCAG CAGGAGGTCC AGCGCCTCCA GGCCCAGCTG
    AGTGAGCTGC AGGCCCAGCT GAGCCAGAAG GAGCAGGCGG CTGAGCACTA CAAGCTACAG
    ATGGAGAAGG CCAAGACTCA TTATGATGCC AAGAAGCAGC AGAACCAAGA GCTGCAGGAG
    CAGCTGCGAG GCCTGGAGCA GCTGCAGACA GAGAACAAGG AGCTGAGGGC AGAAGTGGAT
    CGGCTGGGCC GGGAGCTGCA GCAGGCTGGG CTGAAGACCA AGGAAGCCGA GCAGACCTGC
    CGCCACCTCA CGGCCCAGGT GCGCAGCCTG GAGGCACAGG TTGCCCATGC CGACCAGCAG
    CTTCGGGACC TGGGCAAGTT CCAGGTGGCG ACTGATGCCT TGAAGAGCCG GGAGCCCCAG
    GCTAAGTCTC AGCTGGACTT GAGTATTGAC AGCCTGGATC TGAGCTGCGA GGAGGGGACC
    CCTCTCTCTG TCACCAGCAA GTTGCCTCGC ACCCAGCCAG ACGGCACCAG CATCCCCGGA
    GAGCCAGCCT CCCCCATCTC CCAGCGTCTG CCCCCCAAAG TGGAGTCCCT GGAGAGCCTC
    TACTTCACGC CCATCCCTGC TCGGGGTCAG GCCCCCCTGG AGAGCAGCCT GGACTCCCTG
    GGGGACATCT CCCTGGACTC AAGCCGGAAA ACCCGCTCCG CTCGTAGGCG CACCACGCAG
    ATCATCAACA TCACCATGAC CAAGAAGCTG GACGTGGAGG ATCCAGACAG TGCCAACGCC
    TCCTTCTACA GCACACAGTC AGCCCCCACT TCCCAGGCAG GCCTGAGGGC CGCCTCCTCC
    ACCCAGTCTC TAGCCCGCCT GGGCTCTCCC GACGATGGCA ACTCCACTCT ATTCAGCCTG
    CCTGGCTACC GGCCCACCAC TCGCAGCTCC GCTCGCCGTT CCCAGGCCGG GATGTCCAGT
    GGGGCCCCTC CAGGCAGGAA CAGCTTCTAT GTGGGCACCT GCCAGGACGA GCCCGAACAG
    CTGGATGACT GGAACCGCAT TGCAGAGCTG CAGCAGCGCA ACCGAGTATG CCCACCGCAC
    CTGAAGACCT GCTACCCCCT TGAGTCCAGG CCCTCCCTGA GCCTGCCTGC CATCACAGAT
    GAGGAGATAA AAACCGGCGA CCCCCGGGAG ACCCTGCGCC GAGCCAGCAT GCAGCCAACC
    CAGATAGCTG AGGGCGCTGG CATTACCACA CGGCAGCAGC GCAAGCGGGT TTCCTCAGAG
    ACCCACCAGG GCCCCGGCAC ACCCGAGTCA AAGAAGGCCA CCACATGTTT CCCGCGCCCC
    ATGACCCCCC GGGACCGACA CGAAGGGCGC AGACACAGCA GTACCGAGGC CCAGAAGAAG
    GCAGTTCCAG CTGTTAAACA GGCTGACCGC CGCCAGTCTA TGGCCTTCAG CATCCTCAAC
    ACACCCAAGA AGCTTGGAAA CAGCCTTCTG CGGAAGGCAG CCTCCAAGAA AGCCCCCTCC
    AAAGCCTCAC CCAACCCCCG CAGTGGGACC CGCCGCTCTC CGCGCATCGC CACCACTGCA
    GCCAGCGCTG CCACTGCTGC TGCCAGCGCC GCTGCCACCA CCCCCCGGGC AAAGGGCAAG
    GCGAAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26523
    Clone ID Related Accession (Same CDS sequence) XM_005216176.3
    Accession Version XM_005216176.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6330bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear mitotic apparatus protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104415.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216176.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCGC
    CCGCAGTCTC TTGGGACAGC CAGCCGAGAA GACGCTTGTG GATTGCGAAC CCAGTCCGAG
    GCTACTGAAA AGACTGAGCC AGAAGGCTCT GAACTGCAGG CCCTGCAGGC AGAGGTGAGC
    CGGCTGGAAC AGCAGGCCCG TGAGCACCAG GAGAAGGCCT CCAGCCTGGA GCAAAGCCTT
    GAGTCTGAGC GCGCTGCCCA CGCGGAGCAG GCCAGTACTC TGAAGACTCT GCAGGGCCAG
    TTAGAGCAGA AGGCCCAGGA GCTGGGGAGC AGTCAGGACG CCTTAGCCTC AACCCAGAGG
    GAGTTGGCCA CCCTCCGTGC CAAGGCCCAG GAGCACAGCA AGGCCGAGGA CGAGTGGAAG
    GCCCAGGTGG CCCGGGGTCA GCAGGAGGCC GAGAGGAAAA ACAGCCTCAT CAGCAGCTTG
    GAGGAGGAGG TGTCCATCCT GAACCGCCAG GTGCTGGAGA AGGAGGGCGA GAGCAAGGAG
    CTGAAGCGGC TGGTGGTCGC TGAGTCAGAG AAGAGTCAGA AGCTGGAAGA GCGGCTGCGC
    CTGCTCCAGG CGGAGACCGC CAGCAGCAGC GCCAGGGCTG CGGAACGCAG CTCCGCTCTG
    CGTGAGGAGG TCCAGACCCT CCGGGAGGAG GCTGAGAAGC AGCGGGTGGC TTCTGAGAGC
    CTGCGGCAGG AGCTGGCATC GCAGGCGGAG CGAGCAGAGG AGCTGGGCCA AGAGTTAAAG
    GCCTGGCAGG AGAAGTTCTT TCAGAAGGAG CAAGCCCTCT CGGCCCTGCA GCTGGAGCAC
    ACCAGCACAC AGGCCCTGGC GAGCGAGCTG CTGCCCGTCA AGCACCTGTG CCAGCAGCTG
    CAGGCCGAGC AGGCTGCTGC CGAGAAGCGC CACCGGGAGG AGCTGGAGCA GAGCAAGCAG
    GCGGCTGGCG GGCTGCGAGC AGAGCTGCTG CGGGCCCAGC GCGAGCTCGG GGAGTTGCTG
    CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG CGCATGGCCC AGCAGCTGCG AGCGGAGAAG
    GCCAGCTACG CGGAGCAACT GAGCATGCTG AAGAAGGCTC ACGGCCTCTT GGCGGAGGAG
    AACCGCGGGC TGGGCGAGCG GGCCAGCCTC GGCCGGCAGT TTCTGGAAGT GGAGCTGGAC
    CAGGCCCGCG AGAAGTACAG CCAGGAGCTG GCAGCTGTGC GCGCTGACGC TGAGACCCGT
    CTGGCTGAGA TGCAGCGGGA AGTGCAGAGC ACTGCCCGGG AGCTGGAGGT GATGACCGCC
    AAGTACGAGG GTGCCAAGGC CAAGGTCCTG GAGGAGAGGC AGCGTTTCCA GGAGGAGAGG
    CAGAAACTCA CTGCCCAGGT GGAAGAACTG AGCAAGAAGC TAGCTGACCA TGAGCAAGCC
    AGCAAGGCGC AGCAGCAGAA GCTGAAGGCC CAGGGCGGTG AGAGCCAGCA GGAGGTCCAG
    CGCCTCCAGG CCCAGCTGAG TGAGCTGCAG GCCCAGCTGA GCCAGAAGGA GCAGGCGGCT
    GAGCACTACA AGCTACAGAT GGAGAAGGCC AAGACTCATT ATGATGCCAA GAAGCAGCAG
    AACCAAGAGC TGCAGGAGCA GCTGCGAGGC CTGGAGCAGC TGCAGACAGA GAACAAGGAG
    CTGAGGGCAG AAGTGGATCG GCTGGGCCGG GAGCTGCAGC AGGCTGGGCT GAAGACCAAG
    GAAGCCGAGC AGACCTGCCG CCACCTCACG GCCCAGGTGC GCAGCCTGGA GGCACAGGTT
    GCCCATGCCG ACCAGCAGCT TCGGGACCTG GGCAAGTTCC AGGTGGCGAC TGATGCCTTG
    AAGAGCCGGG AGCCCCAGGC TAAGTCTCAG CTGGACTTGA GTATTGACAG CCTGGATCTG
    AGCTGCGAGG AGGGGACCCC TCTCTCTGTC ACCAGCAAGT TGCCTCGCAC CCAGCCAGAC
    GGCACCAGCA TCCCCGGAGA GCCAGCCTCC CCCATCTCCC AGCGTCTGCC CCCCAAAGTG
    GAGTCCCTGG AGAGCCTCTA CTTCACGCCC ATCCCTGCTC GGGGTCAGGC CCCCCTGGAG
    AGCAGCCTGG ACTCCCTGGG GGACATCTCC CTGGACTCAA GCCGGAAAAC CCGCTCCGCT
    CGTAGGCGCA CCACGCAGAT CATCAACATC ACCATGACCA AGAAGCTGGA CGTGGAGGAT
    CCAGACAGTG CCAACGCCTC CTTCTACAGC ACACAGTCAG CCCCCACTTC CCAGGCAGGC
    CTGAGGGCCG CCTCCTCCAC CCAGTCTCTA GCCCGCCTGG GCTCTCCCGA CGATGGCAAC
    TCCACTCTAT TCAGCCTGCC TGGCTACCGG CCCACCACTC GCAGCTCCGC TCGCCGTTCC
    CAGGCCGGGA TGTCCAGTGG GGCCCCTCCA GGCAGGAACA GCTTCTATGT GGGCACCTGC
    CAGGACGAGC CCGAACAGCT GGATGACTGG AACCGCATTG CAGAGCTGCA GCAGCGCAAC
    CGAGTATGCC CACCGCACCT GAAGACCTGC TACCCCCTTG AGTCCAGGCC CTCCCTGAGC
    CTGCCTGCCA TCACAGATGA GGAGATAAAA ACCGGCGACC CCCGGGAGAC CCTGCGCCGA
    GCCAGCATGC AGCCAACCCA GATAGCTGAG GGCGCTGGCA TTACCACACG GCAGCAGCGC
    AAGCGGGTTT CCTCAGAGAC CCACCAGGGC CCCGGCACAC CCGAGTCAAA GAAGGCCACC
    ACATGTTTCC CGCGCCCCAT GACCCCCCGG GACCGACACG AAGGGCGCAG ACACAGCAGT
    ACCGAGGCCC AGAAGAAGGC AGTTCCAGCT GTTAAACAGG CTGACCGCCG CCAGTCTATG
    GCCTTCAGCA TCCTCAACAC ACCCAAGAAG CTTGGAAACA GCCTTCTGCG GAAGGCAGCC
    TCCAAGAAAG CCCCCTCCAA AGCCTCACCC AACCCCCGCA GTGGGACCCG CCGCTCTCCG
    CGCATCGCCA CCACTGCAGC CAGCGCTGCC ACTGCTGCTG CCAGCGCCGC TGCCACCACC
    CCCCGGGCAA AGGGCAAGGC GAAGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216233.1
    CDS241..6570
    Translation

    Target ORF information:

    RefSeq Version XM_005216176.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216176.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGACGCTCC ATGCCACCCG GGCGGCTGCA CTCCTCACTT GGGTGAACAG TCTGCGCCTG 
    GCTGACCCGG TGGAGGCTGT GCTGCAGCTG CAGGACTGCG GCGTCTTCAT CAGGATCATT
    GACGGCATCC TTGGCACTGA TGAGGGGCAG CAAATCCTGC AGCGGCCGGT GCCGGAGAGA
    CTGGAGTTTG TGTGCAGTTT TCTGCAGAAA AACCGAAAAC ATCCCTCTTC TGCAGAATGC
    TTGGTGTCAG TGCAGAAGGT GATGGAGGGC TCAGAGCTGG AGCTAGCGAA GGTGGCCATG
    CTGCTGCTGT ACCACTCCTC CATGAGCTCC AGGAGTCTCA GGGACTGGGA GCAGTTTGAA
    TACAAGATTC AGGCTGAGTT AGCCGTCATT CTCAAATTCG TGCTGGACCA TGAGGATGGG
    CTAAACCTAA ATGAAGACTT GGAGAGCTTC TTGCAGAAAG CTCCTGTCCC TTCCCCTCGT
    TCCAGCACCA TCTCTGAAGA GCTCTCTCCA CCCAGCCACC AGGCCAAGAG GGAGGTTCAC
    TTCCTAGAGC TACAGAAAGT TGCCTCTTCC AGCGGGAACA ACTTCCTCTC AGGTTCTCCA
    GCCTCCCCCA TGGGTGACAT CTTGCAGACC CCACAGTTCC AGATGAGGCG GTTAAAGAAG
    CAGCTTGCAG ATGAGAGAAA CAATCGAGAC GAGCTGGAGC TGGAGTTGGC TGAGAACCGC
    AAGCTCCTCA CCGAGAAGGA TGCGCAGATA GCCATGATGC AGCAGCGCAT TGACCGCCTC
    ACTCAGCTCA ACGAGAAGCA GGCGGCCAGT CCGCTGGAGC CCAGGGAGCT GGAGGAGCTG
    CGTGGCAAGA ACGAGAGCCT CACTGTGCGG CTGCATGAAA CTCTGAAGCA GTGCCAGGAC
    CTGAAGACAG AGAAGAGCCA GATGGATCGC AGAATTAACC AGCTTTCTGA GGAGAATGGG
    GACCTTTCCT TTAAGCTGCG GGAGTTTGCC AGTCACCTGC AGCAGCTCCA GGGGGCCCTC
    AACGAACTGA CAGAAGAGCA CAGCAGGGCC ACTCAGGAGT GGATGCAGAA GCAGGCGCAT
    CTGGACAAGG AGCTCGGCAC AGCCCTGCAG GACAAGAAAT GCCTTGAAGA GAAGAACGAA
    ATCCTTCAGG GAAAGCTTTC ACAGCTGGAA GAACACTTGG CCCAGCTGCG GGAGAACCCA
    CCCCAGGAGA AGGGTGAGGT GCTGGGTGAC GTCTTGCAGC TGGAAACCCT CAAGCAAGAG
    GCAGCCACTC TCGCTGCAGA CAACACCCAG CTTCAAGCCA GGGTGGCAGC ATTGGAGACC
    GAGCGGGGCC AGCGGGAAGC CAAGCTGCTC GCCGAGCGGA GCCACTTCGA AGGAGAAAAG
    CAGCAGCTGG CCGGCCTGAT TGCCGAGCTG CAGGGCTCCC TGTCCAACCT CAGCCAGGCC
    AAGGAGGAGC TGGAGCAGGC TTCTCAGGCT CAGGTGGCCC GGCTATCCAC CCAGGTGGCC
    ACATTGACCT CCGAGCTGGA TACCCTCAAC ACTGCTGTGC AGCAGCGGGA TGGGGAACTG
    GCCGGCCTGA AGCAGCAGGC CCAAACGGAG CAGGCACAGC TCACACAGAC TCTCCGGCAG
    CAGGAAGAGG CTGCCCAGGG CCTCCGCCAG CAGGTGGAGC AGCTGAGCAG CAGCCTGGAG
    CGGAAGGAGA GGCAGCTGGA AGAGGCCGCC ACGGAGAAGG AGGCCACCCG GCGAGACCAG
    GCCCAGCAAC TGGCTGCTGC GGCCGAGGAG CGGGAGGCTG CTCTCCGGGA GAGAGACACA
    GCCCTCCAGC AGCTGGGGGC GGTGGAGAAG GAGAAGGCTG CCGAGTTGGA CGTCCTGCAA
    GAGCAGCTGC GGGCCGCCCA TGAGGCCCAG GATGGTGCCC AGTCCTCGCT GACACAGGCC
    CAGCAGGAGA AGGCGGAGCT GAGCCAGAAG GTGGAGGAAC TCCATACCCA TGTTGAGGCA
    GCCCGCCAGG AGCAGAGTGA GGTACAGGCC CAGGTGGCAG AGCTGAAGGC CCAGTTGAGG
    TCTGAGCAGC AGAAAACAAC CGAGTGGGAA AGAGTGGCCC AGGAGAAGGC CCAGCTCCAG
    GAGCAGGTCC GGGCCCTGGA GGAGTCCCTT AAGGCCACCA CAGGCAGCCT GGAAAAGGAG
    AAGCGCAGGG CTGCAGACAC CCTGGCAGAG CAGCAGCGGT GCATCTGCAG GTTGGAGGCG
    GAGACGCAGA GCCTGGTGGA GCAGCACGAG CAGGGACAGA AGGAGCTGGA AGAAGAGAGA
    GCTGGGCGCA AGGGGCTGGA GGCCCGGTTA CAGCAGCTCG GGGAGGCCCA TCAGGCCCAG
    ACAGAAGCCC TGCGGCAGGA GCTGGCCGAG GCTGTAGCCT CCCAGCGCGA GGCCGAGAAT
    GAGTGTGAGC AGCTGGCCAA GGAGGTGGCC ACCTGGCGTG AGCGGTATGA GGACAGCCAG
    CAGGAGGAGG AGCAGTATGG CGCCATGTTC CAGGAACAGC TGATGACTCT GAAGGAGGAA
    TGCGAGAAGG CCCGCCAGGA GCTGCAGGAG GCCAAGGAGA AGGTGGTGGG GATCGAGGCC
    CACAGTGAGC TCCAGATCAG CCGGCAGCAG GACGAGCTGG CTCAGCTCCA CGCCAGCTTG
    GCCAGGGCCC TACAGCAGGT CCAAGAGAAG GAGGGCAGGG CCCAGCAGCT CGCCACCGAC
    CTTGCCACCC TGCAGGAGAA GATGGCGGCC ACCAGCAAGG AGGTGGCCCG CCTGGAGGCC
    TTGGTGCGCA AGGCAGGTGG GCAGCCAGAG ACGGCCTCCC CAGAGCTGTT CAAGGAGCCG
    CCCAGGGCAG GAGACAGAGA GTCGGAGTGG CTGGAAGAGC AGCAGGGACG CCCGTTCTGC
    AGCACGCAGG CCGCGCTGCA GGCTATGGAG CGTGAGGCTG AGCAGATGGG TAGCGAGCTG
    GAGAGGCTTC GGGCCGCGCT GATGGAGAGC CAGGGGCAGC AGCAGGAGGA GCGTGGGCAA
    CAGGAGAGGG AGGTGGCGCG GCTGACCCAG GAGCGGGGCC GGGCCCAAGC TGACCTTGCC
    CTGGAGAAGG CCGCCAAGGC GGAGCTGGAG ATGCGGCTGC AGAACGCACT CAATGAGCAG
    CGTGTGGAGT TTGCTGCCCT GCAGGAGGCA CTGGCCCAGG CCCTGAGGGA AAAGGAGGGG
    ATGGACCAGG AACTGGCCAA GCTTCATGGG CAGGAGGCAG CCCAAGGGGC AGTACTGAAG
    GAGCTTCAGC AAACTGTGGA GCGACTGAAA GAACAGCTGG CCAAGAAAGA GGAGGGGCGC
    CCGCAGTCTC TTGGGACAGC CAGCCGAGAA GACGCTTGTG GATTGCGAAC CCAGTCCGAG
    GCTACTGAAA AGACTGAGCC AGAAGGCTCT GAACTGCAGG CCCTGCAGGC AGAGGTGAGC
    CGGCTGGAAC AGCAGGCCCG TGAGCACCAG GAGAAGGCCT CCAGCCTGGA GCAAAGCCTT
    GAGTCTGAGC GCGCTGCCCA CGCGGAGCAG GCCAGTACTC TGAAGACTCT GCAGGGCCAG
    TTAGAGCAGA AGGCCCAGGA GCTGGGGAGC AGTCAGGACG CCTTAGCCTC AACCCAGAGG
    GAGTTGGCCA CCCTCCGTGC CAAGGCCCAG GAGCACAGCA AGGCCGAGGA CGAGTGGAAG
    GCCCAGGTGG CCCGGGGTCA GCAGGAGGCC GAGAGGAAAA ACAGCCTCAT CAGCAGCTTG
    GAGGAGGAGG TGTCCATCCT GAACCGCCAG GTGCTGGAGA AGGAGGGCGA GAGCAAGGAG
    CTGAAGCGGC TGGTGGTCGC TGAGTCAGAG AAGAGTCAGA AGCTGGAAGA GCGGCTGCGC
    CTGCTCCAGG CGGAGACCGC CAGCAGCAGC GCCAGGGCTG CGGAACGCAG CTCCGCTCTG
    CGTGAGGAGG TCCAGACCCT CCGGGAGGAG GCTGAGAAGC AGCGGGTGGC TTCTGAGAGC
    CTGCGGCAGG AGCTGGCATC GCAGGCGGAG CGAGCAGAGG AGCTGGGCCA AGAGTTAAAG
    GCCTGGCAGG AGAAGTTCTT TCAGAAGGAG CAAGCCCTCT CGGCCCTGCA GCTGGAGCAC
    ACCAGCACAC AGGCCCTGGC GAGCGAGCTG CTGCCCGTCA AGCACCTGTG CCAGCAGCTG
    CAGGCCGAGC AGGCTGCTGC CGAGAAGCGC CACCGGGAGG AGCTGGAGCA GAGCAAGCAG
    GCGGCTGGCG GGCTGCGAGC AGAGCTGCTG CGGGCCCAGC GCGAGCTCGG GGAGTTGCTG
    CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG CGCATGGCCC AGCAGCTGCG AGCGGAGAAG
    GCCAGCTACG CGGAGCAACT GAGCATGCTG AAGAAGGCTC ACGGCCTCTT GGCGGAGGAG
    AACCGCGGGC TGGGCGAGCG GGCCAGCCTC GGCCGGCAGT TTCTGGAAGT GGAGCTGGAC
    CAGGCCCGCG AGAAGTACAG CCAGGAGCTG GCAGCTGTGC GCGCTGACGC TGAGACCCGT
    CTGGCTGAGA TGCAGCGGGA AGTGCAGAGC ACTGCCCGGG AGCTGGAGGT GATGACCGCC
    AAGTACGAGG GTGCCAAGGC CAAGGTCCTG GAGGAGAGGC AGCGTTTCCA GGAGGAGAGG
    CAGAAACTCA CTGCCCAGGT GGAAGAACTG AGCAAGAAGC TAGCTGACCA TGAGCAAGCC
    AGCAAGGCGC AGCAGCAGAA GCTGAAGGCC CAGGGCGGTG AGAGCCAGCA GGAGGTCCAG
    CGCCTCCAGG CCCAGCTGAG TGAGCTGCAG GCCCAGCTGA GCCAGAAGGA GCAGGCGGCT
    GAGCACTACA AGCTACAGAT GGAGAAGGCC AAGACTCATT ATGATGCCAA GAAGCAGCAG
    AACCAAGAGC TGCAGGAGCA GCTGCGAGGC CTGGAGCAGC TGCAGACAGA GAACAAGGAG
    CTGAGGGCAG AAGTGGATCG GCTGGGCCGG GAGCTGCAGC AGGCTGGGCT GAAGACCAAG
    GAAGCCGAGC AGACCTGCCG CCACCTCACG GCCCAGGTGC GCAGCCTGGA GGCACAGGTT
    GCCCATGCCG ACCAGCAGCT TCGGGACCTG GGCAAGTTCC AGGTGGCGAC TGATGCCTTG
    AAGAGCCGGG AGCCCCAGGC TAAGTCTCAG CTGGACTTGA GTATTGACAG CCTGGATCTG
    AGCTGCGAGG AGGGGACCCC TCTCTCTGTC ACCAGCAAGT TGCCTCGCAC CCAGCCAGAC
    GGCACCAGCA TCCCCGGAGA GCCAGCCTCC CCCATCTCCC AGCGTCTGCC CCCCAAAGTG
    GAGTCCCTGG AGAGCCTCTA CTTCACGCCC ATCCCTGCTC GGGGTCAGGC CCCCCTGGAG
    AGCAGCCTGG ACTCCCTGGG GGACATCTCC CTGGACTCAA GCCGGAAAAC CCGCTCCGCT
    CGTAGGCGCA CCACGCAGAT CATCAACATC ACCATGACCA AGAAGCTGGA CGTGGAGGAT
    CCAGACAGTG CCAACGCCTC CTTCTACAGC ACACAGTCAG CCCCCACTTC CCAGGCAGGC
    CTGAGGGCCG CCTCCTCCAC CCAGTCTCTA GCCCGCCTGG GCTCTCCCGA CGATGGCAAC
    TCCACTCTAT TCAGCCTGCC TGGCTACCGG CCCACCACTC GCAGCTCCGC TCGCCGTTCC
    CAGGCCGGGA TGTCCAGTGG GGCCCCTCCA GGCAGGAACA GCTTCTATGT GGGCACCTGC
    CAGGACGAGC CCGAACAGCT GGATGACTGG AACCGCATTG CAGAGCTGCA GCAGCGCAAC
    CGAGTATGCC CACCGCACCT GAAGACCTGC TACCCCCTTG AGTCCAGGCC CTCCCTGAGC
    CTGCCTGCCA TCACAGATGA GGAGATAAAA ACCGGCGACC CCCGGGAGAC CCTGCGCCGA
    GCCAGCATGC AGCCAACCCA GATAGCTGAG GGCGCTGGCA TTACCACACG GCAGCAGCGC
    AAGCGGGTTT CCTCAGAGAC CCACCAGGGC CCCGGCACAC CCGAGTCAAA GAAGGCCACC
    ACATGTTTCC CGCGCCCCAT GACCCCCCGG GACCGACACG AAGGGCGCAG ACACAGCAGT
    ACCGAGGCCC AGAAGAAGGC AGTTCCAGCT GTTAAACAGG CTGACCGCCG CCAGTCTATG
    GCCTTCAGCA TCCTCAACAC ACCCAAGAAG CTTGGAAACA GCCTTCTGCG GAAGGCAGCC
    TCCAAGAAAG CCCCCTCCAA AGCCTCACCC AACCCCCGCA GTGGGACCCG CCGCTCTCCG
    CGCATCGCCA CCACTGCAGC CAGCGCTGCC ACTGCTGCTG CCAGCGCCGC TGCCACCACC
    CCCCGGGCAA AGGGCAAGGC GAAGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.