NUMA1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011545054.1	XP_011543356.1	nuclear mitotic apparatus protein 1 isoform X1
XM_011545057.2	XP_011543359.1	nuclear mitotic apparatus protein 1 isoform X1
XM_024448555.1	XP_024304323.1	nuclear mitotic apparatus protein 1 isoform X1
XM_011545059.2	XP_011543361.1	nuclear mitotic apparatus protein 1 isoform X1
XM_011545060.2	XP_011543362.1	nuclear mitotic apparatus protein 1 isoform X1
XM_011545064.1	XP_011543366.1	nuclear mitotic apparatus protein 1 isoform X1
XM_017017830.1	XP_016873319.1	nuclear mitotic apparatus protein 1 isoform X4
XM_011545065.1	XP_011543367.1	nuclear mitotic apparatus protein 1 isoform X2
XM_011545055.1	XP_011543357.1	nuclear mitotic apparatus protein 1 isoform X1
XM_011545056.2	XP_011543358.1	nuclear mitotic apparatus protein 1 isoform X1
XM_011545058.1	XP_011543360.1	nuclear mitotic apparatus protein 1 isoform X1
XM_011545065.2	XP_011543367.1	nuclear mitotic apparatus protein 1 isoform X2
XM_006718564.2	XP_006718627.1	nuclear mitotic apparatus protein 1 isoform X3
XM_017017831.1	XP_016873320.1	nuclear mitotic apparatus protein 1 isoform X4
XM_011545063.2	XP_011543365.1	nuclear mitotic apparatus protein 1 isoform X1
XM_006718564.1	XP_006718627.1	nuclear mitotic apparatus protein 1 isoform X3
XM_011545062.2	XP_011543364.1	nuclear mitotic apparatus protein 1 isoform X1
XM_024448556.1	XP_024304324.1	nuclear mitotic apparatus protein 1 isoform X3
XM_011545066.2	XP_011543368.1	nuclear mitotic apparatus protein 1 isoform X4
XM_011545066.1	XP_011543368.1	nuclear mitotic apparatus protein 1 isoform X4
XM_011545061.2	XP_011543363.1	nuclear mitotic apparatus protein 1 isoform X1
NM_006185.4	NP_006176.2	nuclear mitotic apparatus protein 1 isoform 1
NM_006185.3	NP_006176.2	nuclear mitotic apparatus protein 1 isoform 1
XM_011545054.2	XP_011543356.1	nuclear mitotic apparatus protein 1 isoform X1
XM_011545063.3	XP_011543365.1	nuclear mitotic apparatus protein 1 isoform X1
NM_001286561.1	NP_001273490.1	nuclear mitotic apparatus protein 1 isoform 2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23292	NM_006185.4 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu23292	XM_024448556.1 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu23292	XM_006718564.2 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu23733	NM_001286561.1 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu23733	XM_017017831.1 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu23733	XM_017017830.1 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu23733	XM_011545066.2 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59363	XM_024448555.1 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59363	XM_011545059.2 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59363	XM_011545058.1 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59363	XM_011545064.1 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59363	XM_011545063.3 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59363	XM_011545054.2 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59363	XM_011545061.2 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59363	XM_011545055.1 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59363	XM_011545062.2 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59363	XM_011545056.2 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59363	XM_011545057.2 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59363	XM_011545060.2 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59364	XM_011545065.2 Latest version!	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu59364	XM_011545065.1	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu23292	XM_006718564.1	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu23733	XM_011545066.1	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu23292	NM_006185.3	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu59363	XM_011545054.1	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu59363	XM_011545063.2	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

NUMA1 ( NM_006185.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006176.2 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23292
Clone ID Related Accession (Same CDS sequence)	XM_006718564.2 , XM_006718564.1 , XM_024448556.1 , NM_006185.4 , NM_006185.3
Accession Version	NM_006185.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-29
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG771637.1, BC027493.1, AB210007.1, BG754891.1 and BC004165.2. On Nov 30, 2019 this sequence version replaced NM_006185.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB210007.1, Z11583.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGCAAG 
CTGCCTCGTA CCCAGCCAGA CGGCACCAGC GTCCCTGGAG AACCAGCCTC ACCTATCTCC 
CAGCGCCTGC CCCCCAAGGT AGAATCCCTG GAGAGTCTCT ACTTCACTCC CATCCCTGCT 
CGGAGTCAGG CCCCCCTGGA GAGCAGCCTG GACTCCCTGG GAGACGTCTT CCTGGACTCG 
GGTCGTAAGA CCCGCTCCGC TCGTCGGCGC ACCACGCAGA TCATCAACAT CACCATGACC 
AAGAAGCTAG ATGTGGAAGA GCCAGACAGC GCCAACTCAT CGTTCTACAG CACGCGGTCT 
GCTCCTGCTT CCCAGGCTAG CCTGCGAGCC ACCTCCTCTA CTCAGTCTCT AGCTCGCCTG 
GGTTCTCCCG ATTATGGCAA CTCAGCCCTG CTCAGCTTGC CTGGCTACCG CCCCACCACT 
CGCAGTTCTG CTCGTCGTTC CCAGGCCGGG GTGTCCAGTG GGGCCCCTCC AGGAAGGAAC 
AGCTTCTACA TGGGCACTTG CCAGGATGAG CCTGAGCAGC TGGATGACTG GAACCGCATT 
GCAGAGCTGC AGCAGCGCAA TCGAGTGTGC CCCCCACATC TGAAGACCTG CTATCCCCTG 
GAGTCCAGGC CTTCCCTGAG CCTGGGCACC ATCACAGATG AGGAGATGAA AACTGGAGAC 
CCCCAAGAGA CCCTGCGCCG AGCCAGCATG CAGCCAATCC AGATAGCCGA GGGCACTGGC 
ATCACCACCC GGCAGCAGCG CAAACGGGTC TCCCTAGAGC CCCACCAGGG CCCTGGAACT 
CCTGAGTCTA AGAAGGCCAC CAGCTGTTTC CCACGCCCCA TGACTCCCCG AGACCGACAT 
GAAGGGCGCA AACAGAGCAC TACTGAGGCC CAGAAGAAAG CAGCTCCAGC TTCTACTAAA 
CAGGCTGACC GGCGCCAGTC GATGGCCTTC AGCATCCTCA ACACACCCAA GAAGCTAGGG 
AACAGCCTTC TGCGGCGGGG AGCCTCAAAG AAGGCCCTGT CCAAGGCTTC CCCCAACACT 
CGCAGTGGAA CCCGCCGTTC TCCGCGCATT GCCACCACCA CAGCCAGCGC CGCCACTGCT 
GCCGCCATTG GTGCCACCCC TCGAGCCAAG GGCAAGGCAA AGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006176.2
CDS	188..6535
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	NM_006185.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006185.4

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGCAAG 
CTGCCTCGTA CCCAGCCAGA CGGCACCAGC GTCCCTGGAG AACCAGCCTC ACCTATCTCC 
CAGCGCCTGC CCCCCAAGGT AGAATCCCTG GAGAGTCTCT ACTTCACTCC CATCCCTGCT 
CGGAGTCAGG CCCCCCTGGA GAGCAGCCTG GACTCCCTGG GAGACGTCTT CCTGGACTCG 
GGTCGTAAGA CCCGCTCCGC TCGTCGGCGC ACCACGCAGA TCATCAACAT CACCATGACC 
AAGAAGCTAG ATGTGGAAGA GCCAGACAGC GCCAACTCAT CGTTCTACAG CACGCGGTCT 
GCTCCTGCTT CCCAGGCTAG CCTGCGAGCC ACCTCCTCTA CTCAGTCTCT AGCTCGCCTG 
GGTTCTCCCG ATTATGGCAA CTCAGCCCTG CTCAGCTTGC CTGGCTACCG CCCCACCACT 
CGCAGTTCTG CTCGTCGTTC CCAGGCCGGG GTGTCCAGTG GGGCCCCTCC AGGAAGGAAC 
AGCTTCTACA TGGGCACTTG CCAGGATGAG CCTGAGCAGC TGGATGACTG GAACCGCATT 
GCAGAGCTGC AGCAGCGCAA TCGAGTGTGC CCCCCACATC TGAAGACCTG CTATCCCCTG 
GAGTCCAGGC CTTCCCTGAG CCTGGGCACC ATCACAGATG AGGAGATGAA AACTGGAGAC 
CCCCAAGAGA CCCTGCGCCG AGCCAGCATG CAGCCAATCC AGATAGCCGA GGGCACTGGC 
ATCACCACCC GGCAGCAGCG CAAACGGGTC TCCCTAGAGC CCCACCAGGG CCCTGGAACT 
CCTGAGTCTA AGAAGGCCAC CAGCTGTTTC CCACGCCCCA TGACTCCCCG AGACCGACAT 
GAAGGGCGCA AACAGAGCAC TACTGAGGCC CAGAAGAAAG CAGCTCCAGC TTCTACTAAA 
CAGGCTGACC GGCGCCAGTC GATGGCCTTC AGCATCCTCA ACACACCCAA GAAGCTAGGG 
AACAGCCTTC TGCGGCGGGG AGCCTCAAAG AAGGCCCTGT CCAAGGCTTC CCCCAACACT 
CGCAGTGGAA CCCGCCGTTC TCCGCGCATT GCCACCACCA CAGCCAGCGC CGCCACTGCT 
GCCGCCATTG GTGCCACCCC TCGAGCCAAG GGCAAGGCAA AGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( NM_006185.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006176.2 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant 1, mRNA.

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CloneID	OHu23292
Clone ID Related Accession (Same CDS sequence)	XM_006718564.2 , XM_006718564.1 , XM_024448556.1 , NM_006185.4 , NM_006185.3
Accession Version	NM_006185.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-10
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG771637.1, BC027493.1, AB210007.1, BG754891.1 and BC004165.2. On Nov 8, 2013 this sequence version replaced NM_006185.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.102709.1, AB210007.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGCAAG 
CTGCCTCGTA CCCAGCCAGA CGGCACCAGC GTCCCTGGAG AACCAGCCTC ACCTATCTCC 
CAGCGCCTGC CCCCCAAGGT AGAATCCCTG GAGAGTCTCT ACTTCACTCC CATCCCTGCT 
CGGAGTCAGG CCCCCCTGGA GAGCAGCCTG GACTCCCTGG GAGACGTCTT CCTGGACTCG 
GGTCGTAAGA CCCGCTCCGC TCGTCGGCGC ACCACGCAGA TCATCAACAT CACCATGACC 
AAGAAGCTAG ATGTGGAAGA GCCAGACAGC GCCAACTCAT CGTTCTACAG CACGCGGTCT 
GCTCCTGCTT CCCAGGCTAG CCTGCGAGCC ACCTCCTCTA CTCAGTCTCT AGCTCGCCTG 
GGTTCTCCCG ATTATGGCAA CTCAGCCCTG CTCAGCTTGC CTGGCTACCG CCCCACCACT 
CGCAGTTCTG CTCGTCGTTC CCAGGCCGGG GTGTCCAGTG GGGCCCCTCC AGGAAGGAAC 
AGCTTCTACA TGGGCACTTG CCAGGATGAG CCTGAGCAGC TGGATGACTG GAACCGCATT 
GCAGAGCTGC AGCAGCGCAA TCGAGTGTGC CCCCCACATC TGAAGACCTG CTATCCCCTG 
GAGTCCAGGC CTTCCCTGAG CCTGGGCACC ATCACAGATG AGGAGATGAA AACTGGAGAC 
CCCCAAGAGA CCCTGCGCCG AGCCAGCATG CAGCCAATCC AGATAGCCGA GGGCACTGGC 
ATCACCACCC GGCAGCAGCG CAAACGGGTC TCCCTAGAGC CCCACCAGGG CCCTGGAACT 
CCTGAGTCTA AGAAGGCCAC CAGCTGTTTC CCACGCCCCA TGACTCCCCG AGACCGACAT 
GAAGGGCGCA AACAGAGCAC TACTGAGGCC CAGAAGAAAG CAGCTCCAGC TTCTACTAAA 
CAGGCTGACC GGCGCCAGTC GATGGCCTTC AGCATCCTCA ACACACCCAA GAAGCTAGGG 
AACAGCCTTC TGCGGCGGGG AGCCTCAAAG AAGGCCCTGT CCAAGGCTTC CCCCAACACT 
CGCAGTGGAA CCCGCCGTTC TCCGCGCATT GCCACCACCA CAGCCAGCGC CGCCACTGCT 
GCCGCCATTG GTGCCACCCC TCGAGCCAAG GGCAAGGCAA AGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006176.2
CDS	339..6686
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	NM_006185.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006185.3

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGCAAG 
CTGCCTCGTA CCCAGCCAGA CGGCACCAGC GTCCCTGGAG AACCAGCCTC ACCTATCTCC 
CAGCGCCTGC CCCCCAAGGT AGAATCCCTG GAGAGTCTCT ACTTCACTCC CATCCCTGCT 
CGGAGTCAGG CCCCCCTGGA GAGCAGCCTG GACTCCCTGG GAGACGTCTT CCTGGACTCG 
GGTCGTAAGA CCCGCTCCGC TCGTCGGCGC ACCACGCAGA TCATCAACAT CACCATGACC 
AAGAAGCTAG ATGTGGAAGA GCCAGACAGC GCCAACTCAT CGTTCTACAG CACGCGGTCT 
GCTCCTGCTT CCCAGGCTAG CCTGCGAGCC ACCTCCTCTA CTCAGTCTCT AGCTCGCCTG 
GGTTCTCCCG ATTATGGCAA CTCAGCCCTG CTCAGCTTGC CTGGCTACCG CCCCACCACT 
CGCAGTTCTG CTCGTCGTTC CCAGGCCGGG GTGTCCAGTG GGGCCCCTCC AGGAAGGAAC 
AGCTTCTACA TGGGCACTTG CCAGGATGAG CCTGAGCAGC TGGATGACTG GAACCGCATT 
GCAGAGCTGC AGCAGCGCAA TCGAGTGTGC CCCCCACATC TGAAGACCTG CTATCCCCTG 
GAGTCCAGGC CTTCCCTGAG CCTGGGCACC ATCACAGATG AGGAGATGAA AACTGGAGAC 
CCCCAAGAGA CCCTGCGCCG AGCCAGCATG CAGCCAATCC AGATAGCCGA GGGCACTGGC 
ATCACCACCC GGCAGCAGCG CAAACGGGTC TCCCTAGAGC CCCACCAGGG CCCTGGAACT 
CCTGAGTCTA AGAAGGCCAC CAGCTGTTTC CCACGCCCCA TGACTCCCCG AGACCGACAT 
GAAGGGCGCA AACAGAGCAC TACTGAGGCC CAGAAGAAAG CAGCTCCAGC TTCTACTAAA 
CAGGCTGACC GGCGCCAGTC GATGGCCTTC AGCATCCTCA ACACACCCAA GAAGCTAGGG 
AACAGCCTTC TGCGGCGGGG AGCCTCAAAG AAGGCCCTGT CCAAGGCTTC CCCCAACACT 
CGCAGTGGAA CCCGCCGTTC TCCGCGCATT GCCACCACCA CAGCCAGCGC CGCCACTGCT 
GCCGCCATTG GTGCCACCCC TCGAGCCAAG GGCAAGGCAA AGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_006718564.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718627.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X14, mRNA.

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CloneID	OHu23292
Clone ID Related Accession (Same CDS sequence)	XM_006718564.2 , XM_006718564.1 , XM_024448556.1 , NM_006185.4 , NM_006185.3
Accession Version	XM_006718564.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006718564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGCAAG 
CTGCCTCGTA CCCAGCCAGA CGGCACCAGC GTCCCTGGAG AACCAGCCTC ACCTATCTCC 
CAGCGCCTGC CCCCCAAGGT AGAATCCCTG GAGAGTCTCT ACTTCACTCC CATCCCTGCT 
CGGAGTCAGG CCCCCCTGGA GAGCAGCCTG GACTCCCTGG GAGACGTCTT CCTGGACTCG 
GGTCGTAAGA CCCGCTCCGC TCGTCGGCGC ACCACGCAGA TCATCAACAT CACCATGACC 
AAGAAGCTAG ATGTGGAAGA GCCAGACAGC GCCAACTCAT CGTTCTACAG CACGCGGTCT 
GCTCCTGCTT CCCAGGCTAG CCTGCGAGCC ACCTCCTCTA CTCAGTCTCT AGCTCGCCTG 
GGTTCTCCCG ATTATGGCAA CTCAGCCCTG CTCAGCTTGC CTGGCTACCG CCCCACCACT 
CGCAGTTCTG CTCGTCGTTC CCAGGCCGGG GTGTCCAGTG GGGCCCCTCC AGGAAGGAAC 
AGCTTCTACA TGGGCACTTG CCAGGATGAG CCTGAGCAGC TGGATGACTG GAACCGCATT 
GCAGAGCTGC AGCAGCGCAA TCGAGTGTGC CCCCCACATC TGAAGACCTG CTATCCCCTG 
GAGTCCAGGC CTTCCCTGAG CCTGGGCACC ATCACAGATG AGGAGATGAA AACTGGAGAC 
CCCCAAGAGA CCCTGCGCCG AGCCAGCATG CAGCCAATCC AGATAGCCGA GGGCACTGGC 
ATCACCACCC GGCAGCAGCG CAAACGGGTC TCCCTAGAGC CCCACCAGGG CCCTGGAACT 
CCTGAGTCTA AGAAGGCCAC CAGCTGTTTC CCACGCCCCA TGACTCCCCG AGACCGACAT 
GAAGGGCGCA AACAGAGCAC TACTGAGGCC CAGAAGAAAG CAGCTCCAGC TTCTACTAAA 
CAGGCTGACC GGCGCCAGTC GATGGCCTTC AGCATCCTCA ACACACCCAA GAAGCTAGGG 
AACAGCCTTC TGCGGCGGGG AGCCTCAAAG AAGGCCCTGT CCAAGGCTTC CCCCAACACT 
CGCAGTGGAA CCCGCCGTTC TCCGCGCATT GCCACCACCA CAGCCAGCGC CGCCACTGCT 
GCCGCCATTG GTGCCACCCC TCGAGCCAAG GGCAAGGCAA AGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718627.1
CDS	161..6508
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_006718564.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718564.2

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGCAAG 
CTGCCTCGTA CCCAGCCAGA CGGCACCAGC GTCCCTGGAG AACCAGCCTC ACCTATCTCC 
CAGCGCCTGC CCCCCAAGGT AGAATCCCTG GAGAGTCTCT ACTTCACTCC CATCCCTGCT 
CGGAGTCAGG CCCCCCTGGA GAGCAGCCTG GACTCCCTGG GAGACGTCTT CCTGGACTCG 
GGTCGTAAGA CCCGCTCCGC TCGTCGGCGC ACCACGCAGA TCATCAACAT CACCATGACC 
AAGAAGCTAG ATGTGGAAGA GCCAGACAGC GCCAACTCAT CGTTCTACAG CACGCGGTCT 
GCTCCTGCTT CCCAGGCTAG CCTGCGAGCC ACCTCCTCTA CTCAGTCTCT AGCTCGCCTG 
GGTTCTCCCG ATTATGGCAA CTCAGCCCTG CTCAGCTTGC CTGGCTACCG CCCCACCACT 
CGCAGTTCTG CTCGTCGTTC CCAGGCCGGG GTGTCCAGTG GGGCCCCTCC AGGAAGGAAC 
AGCTTCTACA TGGGCACTTG CCAGGATGAG CCTGAGCAGC TGGATGACTG GAACCGCATT 
GCAGAGCTGC AGCAGCGCAA TCGAGTGTGC CCCCCACATC TGAAGACCTG CTATCCCCTG 
GAGTCCAGGC CTTCCCTGAG CCTGGGCACC ATCACAGATG AGGAGATGAA AACTGGAGAC 
CCCCAAGAGA CCCTGCGCCG AGCCAGCATG CAGCCAATCC AGATAGCCGA GGGCACTGGC 
ATCACCACCC GGCAGCAGCG CAAACGGGTC TCCCTAGAGC CCCACCAGGG CCCTGGAACT 
CCTGAGTCTA AGAAGGCCAC CAGCTGTTTC CCACGCCCCA TGACTCCCCG AGACCGACAT 
GAAGGGCGCA AACAGAGCAC TACTGAGGCC CAGAAGAAAG CAGCTCCAGC TTCTACTAAA 
CAGGCTGACC GGCGCCAGTC GATGGCCTTC AGCATCCTCA ACACACCCAA GAAGCTAGGG 
AACAGCCTTC TGCGGCGGGG AGCCTCAAAG AAGGCCCTGT CCAAGGCTTC CCCCAACACT 
CGCAGTGGAA CCCGCCGTTC TCCGCGCATT GCCACCACCA CAGCCAGCGC CGCCACTGCT 
GCCGCCATTG GTGCCACCCC TCGAGCCAAG GGCAAGGCAA AGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_006718564.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718627.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X13, mRNA.

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CloneID	OHu23292
Clone ID Related Accession (Same CDS sequence)	XM_006718564.2 , XM_006718564.1 , XM_024448556.1 , NM_006185.4 , NM_006185.3
Accession Version	XM_006718564.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGCAAG 
CTGCCTCGTA CCCAGCCAGA CGGCACCAGC GTCCCTGGAG AACCAGCCTC ACCTATCTCC 
CAGCGCCTGC CCCCCAAGGT AGAATCCCTG GAGAGTCTCT ACTTCACTCC CATCCCTGCT 
CGGAGTCAGG CCCCCCTGGA GAGCAGCCTG GACTCCCTGG GAGACGTCTT CCTGGACTCG 
GGTCGTAAGA CCCGCTCCGC TCGTCGGCGC ACCACGCAGA TCATCAACAT CACCATGACC 
AAGAAGCTAG ATGTGGAAGA GCCAGACAGC GCCAACTCAT CGTTCTACAG CACGCGGTCT 
GCTCCTGCTT CCCAGGCTAG CCTGCGAGCC ACCTCCTCTA CTCAGTCTCT AGCTCGCCTG 
GGTTCTCCCG ATTATGGCAA CTCAGCCCTG CTCAGCTTGC CTGGCTACCG CCCCACCACT 
CGCAGTTCTG CTCGTCGTTC CCAGGCCGGG GTGTCCAGTG GGGCCCCTCC AGGAAGGAAC 
AGCTTCTACA TGGGCACTTG CCAGGATGAG CCTGAGCAGC TGGATGACTG GAACCGCATT 
GCAGAGCTGC AGCAGCGCAA TCGAGTGTGC CCCCCACATC TGAAGACCTG CTATCCCCTG 
GAGTCCAGGC CTTCCCTGAG CCTGGGCACC ATCACAGATG AGGAGATGAA AACTGGAGAC 
CCCCAAGAGA CCCTGCGCCG AGCCAGCATG CAGCCAATCC AGATAGCCGA GGGCACTGGC 
ATCACCACCC GGCAGCAGCG CAAACGGGTC TCCCTAGAGC CCCACCAGGG CCCTGGAACT 
CCTGAGTCTA AGAAGGCCAC CAGCTGTTTC CCACGCCCCA TGACTCCCCG AGACCGACAT 
GAAGGGCGCA AACAGAGCAC TACTGAGGCC CAGAAGAAAG CAGCTCCAGC TTCTACTAAA 
CAGGCTGACC GGCGCCAGTC GATGGCCTTC AGCATCCTCA ACACACCCAA GAAGCTAGGG 
AACAGCCTTC TGCGGCGGGG AGCCTCAAAG AAGGCCCTGT CCAAGGCTTC CCCCAACACT 
CGCAGTGGAA CCCGCCGTTC TCCGCGCATT GCCACCACCA CAGCCAGCGC CGCCACTGCT 
GCCGCCATTG GTGCCACCCC TCGAGCCAAG GGCAAGGCAA AGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718627.1
CDS	154..6501
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_006718564.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718564.1

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGCAAG 
CTGCCTCGTA CCCAGCCAGA CGGCACCAGC GTCCCTGGAG AACCAGCCTC ACCTATCTCC 
CAGCGCCTGC CCCCCAAGGT AGAATCCCTG GAGAGTCTCT ACTTCACTCC CATCCCTGCT 
CGGAGTCAGG CCCCCCTGGA GAGCAGCCTG GACTCCCTGG GAGACGTCTT CCTGGACTCG 
GGTCGTAAGA CCCGCTCCGC TCGTCGGCGC ACCACGCAGA TCATCAACAT CACCATGACC 
AAGAAGCTAG ATGTGGAAGA GCCAGACAGC GCCAACTCAT CGTTCTACAG CACGCGGTCT 
GCTCCTGCTT CCCAGGCTAG CCTGCGAGCC ACCTCCTCTA CTCAGTCTCT AGCTCGCCTG 
GGTTCTCCCG ATTATGGCAA CTCAGCCCTG CTCAGCTTGC CTGGCTACCG CCCCACCACT 
CGCAGTTCTG CTCGTCGTTC CCAGGCCGGG GTGTCCAGTG GGGCCCCTCC AGGAAGGAAC 
AGCTTCTACA TGGGCACTTG CCAGGATGAG CCTGAGCAGC TGGATGACTG GAACCGCATT 
GCAGAGCTGC AGCAGCGCAA TCGAGTGTGC CCCCCACATC TGAAGACCTG CTATCCCCTG 
GAGTCCAGGC CTTCCCTGAG CCTGGGCACC ATCACAGATG AGGAGATGAA AACTGGAGAC 
CCCCAAGAGA CCCTGCGCCG AGCCAGCATG CAGCCAATCC AGATAGCCGA GGGCACTGGC 
ATCACCACCC GGCAGCAGCG CAAACGGGTC TCCCTAGAGC CCCACCAGGG CCCTGGAACT 
CCTGAGTCTA AGAAGGCCAC CAGCTGTTTC CCACGCCCCA TGACTCCCCG AGACCGACAT 
GAAGGGCGCA AACAGAGCAC TACTGAGGCC CAGAAGAAAG CAGCTCCAGC TTCTACTAAA 
CAGGCTGACC GGCGCCAGTC GATGGCCTTC AGCATCCTCA ACACACCCAA GAAGCTAGGG 
AACAGCCTTC TGCGGCGGGG AGCCTCAAAG AAGGCCCTGT CCAAGGCTTC CCCCAACACT 
CGCAGTGGAA CCCGCCGTTC TCCGCGCATT GCCACCACCA CAGCCAGCGC CGCCACTGCT 
GCCGCCATTG GTGCCACCCC TCGAGCCAAG GGCAAGGCAA AGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_024448556.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304324.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X15, mRNA.

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CloneID	OHu23292
Clone ID Related Accession (Same CDS sequence)	XM_006718564.2 , XM_006718564.1 , XM_024448556.1 , NM_006185.4 , NM_006185.3
Accession Version	XM_024448556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGCAAG 
CTGCCTCGTA CCCAGCCAGA CGGCACCAGC GTCCCTGGAG AACCAGCCTC ACCTATCTCC 
CAGCGCCTGC CCCCCAAGGT AGAATCCCTG GAGAGTCTCT ACTTCACTCC CATCCCTGCT 
CGGAGTCAGG CCCCCCTGGA GAGCAGCCTG GACTCCCTGG GAGACGTCTT CCTGGACTCG 
GGTCGTAAGA CCCGCTCCGC TCGTCGGCGC ACCACGCAGA TCATCAACAT CACCATGACC 
AAGAAGCTAG ATGTGGAAGA GCCAGACAGC GCCAACTCAT CGTTCTACAG CACGCGGTCT 
GCTCCTGCTT CCCAGGCTAG CCTGCGAGCC ACCTCCTCTA CTCAGTCTCT AGCTCGCCTG 
GGTTCTCCCG ATTATGGCAA CTCAGCCCTG CTCAGCTTGC CTGGCTACCG CCCCACCACT 
CGCAGTTCTG CTCGTCGTTC CCAGGCCGGG GTGTCCAGTG GGGCCCCTCC AGGAAGGAAC 
AGCTTCTACA TGGGCACTTG CCAGGATGAG CCTGAGCAGC TGGATGACTG GAACCGCATT 
GCAGAGCTGC AGCAGCGCAA TCGAGTGTGC CCCCCACATC TGAAGACCTG CTATCCCCTG 
GAGTCCAGGC CTTCCCTGAG CCTGGGCACC ATCACAGATG AGGAGATGAA AACTGGAGAC 
CCCCAAGAGA CCCTGCGCCG AGCCAGCATG CAGCCAATCC AGATAGCCGA GGGCACTGGC 
ATCACCACCC GGCAGCAGCG CAAACGGGTC TCCCTAGAGC CCCACCAGGG CCCTGGAACT 
CCTGAGTCTA AGAAGGCCAC CAGCTGTTTC CCACGCCCCA TGACTCCCCG AGACCGACAT 
GAAGGGCGCA AACAGAGCAC TACTGAGGCC CAGAAGAAAG CAGCTCCAGC TTCTACTAAA 
CAGGCTGACC GGCGCCAGTC GATGGCCTTC AGCATCCTCA ACACACCCAA GAAGCTAGGG 
AACAGCCTTC TGCGGCGGGG AGCCTCAAAG AAGGCCCTGT CCAAGGCTTC CCCCAACACT 
CGCAGTGGAA CCCGCCGTTC TCCGCGCATT GCCACCACCA CAGCCAGCGC CGCCACTGCT 
GCCGCCATTG GTGCCACCCC TCGAGCCAAG GGCAAGGCAA AGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304324.1
CDS	258..6605
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_024448556.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448556.1

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGCAAG 
CTGCCTCGTA CCCAGCCAGA CGGCACCAGC GTCCCTGGAG AACCAGCCTC ACCTATCTCC 
CAGCGCCTGC CCCCCAAGGT AGAATCCCTG GAGAGTCTCT ACTTCACTCC CATCCCTGCT 
CGGAGTCAGG CCCCCCTGGA GAGCAGCCTG GACTCCCTGG GAGACGTCTT CCTGGACTCG 
GGTCGTAAGA CCCGCTCCGC TCGTCGGCGC ACCACGCAGA TCATCAACAT CACCATGACC 
AAGAAGCTAG ATGTGGAAGA GCCAGACAGC GCCAACTCAT CGTTCTACAG CACGCGGTCT 
GCTCCTGCTT CCCAGGCTAG CCTGCGAGCC ACCTCCTCTA CTCAGTCTCT AGCTCGCCTG 
GGTTCTCCCG ATTATGGCAA CTCAGCCCTG CTCAGCTTGC CTGGCTACCG CCCCACCACT 
CGCAGTTCTG CTCGTCGTTC CCAGGCCGGG GTGTCCAGTG GGGCCCCTCC AGGAAGGAAC 
AGCTTCTACA TGGGCACTTG CCAGGATGAG CCTGAGCAGC TGGATGACTG GAACCGCATT 
GCAGAGCTGC AGCAGCGCAA TCGAGTGTGC CCCCCACATC TGAAGACCTG CTATCCCCTG 
GAGTCCAGGC CTTCCCTGAG CCTGGGCACC ATCACAGATG AGGAGATGAA AACTGGAGAC 
CCCCAAGAGA CCCTGCGCCG AGCCAGCATG CAGCCAATCC AGATAGCCGA GGGCACTGGC 
ATCACCACCC GGCAGCAGCG CAAACGGGTC TCCCTAGAGC CCCACCAGGG CCCTGGAACT 
CCTGAGTCTA AGAAGGCCAC CAGCTGTTTC CCACGCCCCA TGACTCCCCG AGACCGACAT 
GAAGGGCGCA AACAGAGCAC TACTGAGGCC CAGAAGAAAG CAGCTCCAGC TTCTACTAAA 
CAGGCTGACC GGCGCCAGTC GATGGCCTTC AGCATCCTCA ACACACCCAA GAAGCTAGGG 
AACAGCCTTC TGCGGCGGGG AGCCTCAAAG AAGGCCCTGT CCAAGGCTTC CCCCAACACT 
CGCAGTGGAA CCCGCCGTTC TCCGCGCATT GCCACCACCA CAGCCAGCGC CGCCACTGCT 
GCCGCCATTG GTGCCACCCC TCGAGCCAAG GGCAAGGCAA AGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( NM_001286561.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273490.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant 2, mRNA.

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CloneID	OHu23733
Clone ID Related Accession (Same CDS sequence)	XM_017017830.1 , XM_017017831.1 , XM_011545066.2 , XM_011545066.1 , NM_001286561.1
Accession Version	NM_001286561.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG771637.1, Z11584.1, Z11583.1, AB210007.1, BG754891.1 and BC004165.2. On Nov 8, 2013 this sequence version replaced XM_005274016.1. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z11584.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAAGAACTG 
AGTAAGAAAC TGGCTGACTC TGACCAAGCC AGCAAGGTGC AGCAGCAGAA GCTGAAGGCT 
GTCCAGGCTC AGGGAGGCGA GAGCCAGCAG GAGGCCCAGC GCCTCCAGGC CCAGCTGAAT 
GAACTGCAAG CCCAGTTGAG CCAGAAGGAG CAGGCAGCTG AGCACTATAA GCTGCAGATG 
GAGAAAGCCA AAACACATTA TGATGCCAAG AAGCAGCAGA ACCAAGAGCT GCAGGAGCAG 
CTGCGGAGCC TGGAGCAGCT GCAGAAGGAA AACAAAGAGC TGCGAGCTGA AGCTGAACGG 
CTGGGCCATG AGCTACAGCA GGCTGGGCTG AAGACCAAGG AGGCTGAACA GACCTGCCGC 
CACCTTACTG CCCAGGTGCG CAGCCTGGAG GCACAGGTTG CCCATGCAGA CCAGCAGCTT 
CGAGACCTGG GCAAATTCCA GGTGGCAACT GATGCTTTAA AGAGCCGTGA GCCCCAGGCT 
AAGCCCCAGC TGGACTTGAG TATTGACAGC CTGGATCTGA GCTGCGAGGA GGGGACCCCA 
CTCAGTATCA CCAGCAAGCT GCCTCGTACC CAGCCAGACG GCACCAGCGT CCCTGGAGAA 
CCAGCCTCAC CTATCTCCCA GCGCCTGCCC CCCAAGGTAG AATCCCTGGA GAGTCTCTAC 
TTCACTCCCA TCCCTGCTCG GAGTCAGGCC CCCCTGGAGA GCAGCCTGGA CTCCCTGGGA 
GACGTCTTCC TGGACTCGGG TCGTAAGACC CGCTCCGCTC GTCGGCGCAC CACGCAGATC 
ATCAACATCA CCATGACCAA GAAGCTAGAT GTGGAAGAGC CAGACAGCGC CAACTCATCG 
TTCTACAGCA CGCGGTCTGC TCCTGCTTCC CAGGCTAGCC TGCGAGCCAC CTCCTCTACT 
CAGTCTCTAG CTCGCCTGGG TTCTCCCGAT TATGGCAACT CAGCCCTGCT CAGCTTGCCT 
GGCTACCGCC CCACCACTCG CAGTTCTGCT CGTCGTTCCC AGGCCGGGGT GTCCAGTGGG 
GCCCCTCCAG GAAGGAACAG CTTCTACATG GGCACTTGCC AGGATGAGCC TGAGCAGCTG 
GATGACTGGA ACCGCATTGC AGAGCTGCAG CAGCGCAATC GAGTGTGCCC CCCACATCTG 
AAGACCTGCT ATCCCCTGGA GTCCAGGCCT TCCCTGAGCC TGGGCACCAT CACAGATGAG 
GAGATGAAAA CTGGAGACCC CCAAGAGACC CTGCGCCGAG CCAGCATGCA GCCAATCCAG 
ATAGCCGAGG GCACTGGCAT CACCACCCGG CAGCAGCGCA AACGGGTCTC CCTAGAGCCC 
CACCAGGGCC CTGGAACTCC TGAGTCTAAG AAGGCCACCA GCTGTTTCCC ACGCCCCATG 
ACTCCCCGAG ACCGACATGA AGGGCGCAAA CAGAGCACTA CTGAGGCCCA GAAGAAAGCA 
GCTCCAGCTT CTACTAAACA GGCTGACCGG CGCCAGTCGA TGGCCTTCAG CATCCTCAAC 
ACACCCAAGA AGCTAGGGAA CAGCCTTCTG CGGCGGGGAG CCTCAAAGAA GGCCCTGTCC 
AAGGCTTCCC CCAACACTCG CAGTGGAACC CGCCGTTCTC CGCGCATTGC CACCACCACA 
GCCAGCGCCG CCACTGCTGC CGCCATTGGT GCCACCCCTC GAGCCAAGGG CAAGGCAAAG 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273490.1
CDS	437..6742
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	NM_001286561.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286561.1

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAAGAACTG 
AGTAAGAAAC TGGCTGACTC TGACCAAGCC AGCAAGGTGC AGCAGCAGAA GCTGAAGGCT 
GTCCAGGCTC AGGGAGGCGA GAGCCAGCAG GAGGCCCAGC GCCTCCAGGC CCAGCTGAAT 
GAACTGCAAG CCCAGTTGAG CCAGAAGGAG CAGGCAGCTG AGCACTATAA GCTGCAGATG 
GAGAAAGCCA AAACACATTA TGATGCCAAG AAGCAGCAGA ACCAAGAGCT GCAGGAGCAG 
CTGCGGAGCC TGGAGCAGCT GCAGAAGGAA AACAAAGAGC TGCGAGCTGA AGCTGAACGG 
CTGGGCCATG AGCTACAGCA GGCTGGGCTG AAGACCAAGG AGGCTGAACA GACCTGCCGC 
CACCTTACTG CCCAGGTGCG CAGCCTGGAG GCACAGGTTG CCCATGCAGA CCAGCAGCTT 
CGAGACCTGG GCAAATTCCA GGTGGCAACT GATGCTTTAA AGAGCCGTGA GCCCCAGGCT 
AAGCCCCAGC TGGACTTGAG TATTGACAGC CTGGATCTGA GCTGCGAGGA GGGGACCCCA 
CTCAGTATCA CCAGCAAGCT GCCTCGTACC CAGCCAGACG GCACCAGCGT CCCTGGAGAA 
CCAGCCTCAC CTATCTCCCA GCGCCTGCCC CCCAAGGTAG AATCCCTGGA GAGTCTCTAC 
TTCACTCCCA TCCCTGCTCG GAGTCAGGCC CCCCTGGAGA GCAGCCTGGA CTCCCTGGGA 
GACGTCTTCC TGGACTCGGG TCGTAAGACC CGCTCCGCTC GTCGGCGCAC CACGCAGATC 
ATCAACATCA CCATGACCAA GAAGCTAGAT GTGGAAGAGC CAGACAGCGC CAACTCATCG 
TTCTACAGCA CGCGGTCTGC TCCTGCTTCC CAGGCTAGCC TGCGAGCCAC CTCCTCTACT 
CAGTCTCTAG CTCGCCTGGG TTCTCCCGAT TATGGCAACT CAGCCCTGCT CAGCTTGCCT 
GGCTACCGCC CCACCACTCG CAGTTCTGCT CGTCGTTCCC AGGCCGGGGT GTCCAGTGGG 
GCCCCTCCAG GAAGGAACAG CTTCTACATG GGCACTTGCC AGGATGAGCC TGAGCAGCTG 
GATGACTGGA ACCGCATTGC AGAGCTGCAG CAGCGCAATC GAGTGTGCCC CCCACATCTG 
AAGACCTGCT ATCCCCTGGA GTCCAGGCCT TCCCTGAGCC TGGGCACCAT CACAGATGAG 
GAGATGAAAA CTGGAGACCC CCAAGAGACC CTGCGCCGAG CCAGCATGCA GCCAATCCAG 
ATAGCCGAGG GCACTGGCAT CACCACCCGG CAGCAGCGCA AACGGGTCTC CCTAGAGCCC 
CACCAGGGCC CTGGAACTCC TGAGTCTAAG AAGGCCACCA GCTGTTTCCC ACGCCCCATG 
ACTCCCCGAG ACCGACATGA AGGGCGCAAA CAGAGCACTA CTGAGGCCCA GAAGAAAGCA 
GCTCCAGCTT CTACTAAACA GGCTGACCGG CGCCAGTCGA TGGCCTTCAG CATCCTCAAC 
ACACCCAAGA AGCTAGGGAA CAGCCTTCTG CGGCGGGGAG CCTCAAAGAA GGCCCTGTCC 
AAGGCTTCCC CCAACACTCG CAGTGGAACC CGCCGTTCTC CGCGCATTGC CACCACCACA 
GCCAGCGCCG CCACTGCTGC CGCCATTGGT GCCACCCCTC GAGCCAAGGG CAAGGCAAAG 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_017017830.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873319.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X17, mRNA.

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CloneID	OHu23733
Clone ID Related Accession (Same CDS sequence)	XM_017017830.1 , XM_017017831.1 , XM_011545066.2 , XM_011545066.1 , NM_001286561.1
Accession Version	XM_017017830.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAAGAACTG 
AGTAAGAAAC TGGCTGACTC TGACCAAGCC AGCAAGGTGC AGCAGCAGAA GCTGAAGGCT 
GTCCAGGCTC AGGGAGGCGA GAGCCAGCAG GAGGCCCAGC GCCTCCAGGC CCAGCTGAAT 
GAACTGCAAG CCCAGTTGAG CCAGAAGGAG CAGGCAGCTG AGCACTATAA GCTGCAGATG 
GAGAAAGCCA AAACACATTA TGATGCCAAG AAGCAGCAGA ACCAAGAGCT GCAGGAGCAG 
CTGCGGAGCC TGGAGCAGCT GCAGAAGGAA AACAAAGAGC TGCGAGCTGA AGCTGAACGG 
CTGGGCCATG AGCTACAGCA GGCTGGGCTG AAGACCAAGG AGGCTGAACA GACCTGCCGC 
CACCTTACTG CCCAGGTGCG CAGCCTGGAG GCACAGGTTG CCCATGCAGA CCAGCAGCTT 
CGAGACCTGG GCAAATTCCA GGTGGCAACT GATGCTTTAA AGAGCCGTGA GCCCCAGGCT 
AAGCCCCAGC TGGACTTGAG TATTGACAGC CTGGATCTGA GCTGCGAGGA GGGGACCCCA 
CTCAGTATCA CCAGCAAGCT GCCTCGTACC CAGCCAGACG GCACCAGCGT CCCTGGAGAA 
CCAGCCTCAC CTATCTCCCA GCGCCTGCCC CCCAAGGTAG AATCCCTGGA GAGTCTCTAC 
TTCACTCCCA TCCCTGCTCG GAGTCAGGCC CCCCTGGAGA GCAGCCTGGA CTCCCTGGGA 
GACGTCTTCC TGGACTCGGG TCGTAAGACC CGCTCCGCTC GTCGGCGCAC CACGCAGATC 
ATCAACATCA CCATGACCAA GAAGCTAGAT GTGGAAGAGC CAGACAGCGC CAACTCATCG 
TTCTACAGCA CGCGGTCTGC TCCTGCTTCC CAGGCTAGCC TGCGAGCCAC CTCCTCTACT 
CAGTCTCTAG CTCGCCTGGG TTCTCCCGAT TATGGCAACT CAGCCCTGCT CAGCTTGCCT 
GGCTACCGCC CCACCACTCG CAGTTCTGCT CGTCGTTCCC AGGCCGGGGT GTCCAGTGGG 
GCCCCTCCAG GAAGGAACAG CTTCTACATG GGCACTTGCC AGGATGAGCC TGAGCAGCTG 
GATGACTGGA ACCGCATTGC AGAGCTGCAG CAGCGCAATC GAGTGTGCCC CCCACATCTG 
AAGACCTGCT ATCCCCTGGA GTCCAGGCCT TCCCTGAGCC TGGGCACCAT CACAGATGAG 
GAGATGAAAA CTGGAGACCC CCAAGAGACC CTGCGCCGAG CCAGCATGCA GCCAATCCAG 
ATAGCCGAGG GCACTGGCAT CACCACCCGG CAGCAGCGCA AACGGGTCTC CCTAGAGCCC 
CACCAGGGCC CTGGAACTCC TGAGTCTAAG AAGGCCACCA GCTGTTTCCC ACGCCCCATG 
ACTCCCCGAG ACCGACATGA AGGGCGCAAA CAGAGCACTA CTGAGGCCCA GAAGAAAGCA 
GCTCCAGCTT CTACTAAACA GGCTGACCGG CGCCAGTCGA TGGCCTTCAG CATCCTCAAC 
ACACCCAAGA AGCTAGGGAA CAGCCTTCTG CGGCGGGGAG CCTCAAAGAA GGCCCTGTCC 
AAGGCTTCCC CCAACACTCG CAGTGGAACC CGCCGTTCTC CGCGCATTGC CACCACCACA 
GCCAGCGCCG CCACTGCTGC CGCCATTGGT GCCACCCCTC GAGCCAAGGG CAAGGCAAAG 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873319.1
CDS	194..6499
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_017017830.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017830.1

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAAGAACTG 
AGTAAGAAAC TGGCTGACTC TGACCAAGCC AGCAAGGTGC AGCAGCAGAA GCTGAAGGCT 
GTCCAGGCTC AGGGAGGCGA GAGCCAGCAG GAGGCCCAGC GCCTCCAGGC CCAGCTGAAT 
GAACTGCAAG CCCAGTTGAG CCAGAAGGAG CAGGCAGCTG AGCACTATAA GCTGCAGATG 
GAGAAAGCCA AAACACATTA TGATGCCAAG AAGCAGCAGA ACCAAGAGCT GCAGGAGCAG 
CTGCGGAGCC TGGAGCAGCT GCAGAAGGAA AACAAAGAGC TGCGAGCTGA AGCTGAACGG 
CTGGGCCATG AGCTACAGCA GGCTGGGCTG AAGACCAAGG AGGCTGAACA GACCTGCCGC 
CACCTTACTG CCCAGGTGCG CAGCCTGGAG GCACAGGTTG CCCATGCAGA CCAGCAGCTT 
CGAGACCTGG GCAAATTCCA GGTGGCAACT GATGCTTTAA AGAGCCGTGA GCCCCAGGCT 
AAGCCCCAGC TGGACTTGAG TATTGACAGC CTGGATCTGA GCTGCGAGGA GGGGACCCCA 
CTCAGTATCA CCAGCAAGCT GCCTCGTACC CAGCCAGACG GCACCAGCGT CCCTGGAGAA 
CCAGCCTCAC CTATCTCCCA GCGCCTGCCC CCCAAGGTAG AATCCCTGGA GAGTCTCTAC 
TTCACTCCCA TCCCTGCTCG GAGTCAGGCC CCCCTGGAGA GCAGCCTGGA CTCCCTGGGA 
GACGTCTTCC TGGACTCGGG TCGTAAGACC CGCTCCGCTC GTCGGCGCAC CACGCAGATC 
ATCAACATCA CCATGACCAA GAAGCTAGAT GTGGAAGAGC CAGACAGCGC CAACTCATCG 
TTCTACAGCA CGCGGTCTGC TCCTGCTTCC CAGGCTAGCC TGCGAGCCAC CTCCTCTACT 
CAGTCTCTAG CTCGCCTGGG TTCTCCCGAT TATGGCAACT CAGCCCTGCT CAGCTTGCCT 
GGCTACCGCC CCACCACTCG CAGTTCTGCT CGTCGTTCCC AGGCCGGGGT GTCCAGTGGG 
GCCCCTCCAG GAAGGAACAG CTTCTACATG GGCACTTGCC AGGATGAGCC TGAGCAGCTG 
GATGACTGGA ACCGCATTGC AGAGCTGCAG CAGCGCAATC GAGTGTGCCC CCCACATCTG 
AAGACCTGCT ATCCCCTGGA GTCCAGGCCT TCCCTGAGCC TGGGCACCAT CACAGATGAG 
GAGATGAAAA CTGGAGACCC CCAAGAGACC CTGCGCCGAG CCAGCATGCA GCCAATCCAG 
ATAGCCGAGG GCACTGGCAT CACCACCCGG CAGCAGCGCA AACGGGTCTC CCTAGAGCCC 
CACCAGGGCC CTGGAACTCC TGAGTCTAAG AAGGCCACCA GCTGTTTCCC ACGCCCCATG 
ACTCCCCGAG ACCGACATGA AGGGCGCAAA CAGAGCACTA CTGAGGCCCA GAAGAAAGCA 
GCTCCAGCTT CTACTAAACA GGCTGACCGG CGCCAGTCGA TGGCCTTCAG CATCCTCAAC 
ACACCCAAGA AGCTAGGGAA CAGCCTTCTG CGGCGGGGAG CCTCAAAGAA GGCCCTGTCC 
AAGGCTTCCC CCAACACTCG CAGTGGAACC CGCCGTTCTC CGCGCATTGC CACCACCACA 
GCCAGCGCCG CCACTGCTGC CGCCATTGGT GCCACCCCTC GAGCCAAGGG CAAGGCAAAG 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_017017831.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873320.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X18, mRNA.

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CloneID	OHu23733
Clone ID Related Accession (Same CDS sequence)	XM_017017830.1 , XM_017017831.1 , XM_011545066.2 , XM_011545066.1 , NM_001286561.1
Accession Version	XM_017017831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAAGAACTG 
AGTAAGAAAC TGGCTGACTC TGACCAAGCC AGCAAGGTGC AGCAGCAGAA GCTGAAGGCT 
GTCCAGGCTC AGGGAGGCGA GAGCCAGCAG GAGGCCCAGC GCCTCCAGGC CCAGCTGAAT 
GAACTGCAAG CCCAGTTGAG CCAGAAGGAG CAGGCAGCTG AGCACTATAA GCTGCAGATG 
GAGAAAGCCA AAACACATTA TGATGCCAAG AAGCAGCAGA ACCAAGAGCT GCAGGAGCAG 
CTGCGGAGCC TGGAGCAGCT GCAGAAGGAA AACAAAGAGC TGCGAGCTGA AGCTGAACGG 
CTGGGCCATG AGCTACAGCA GGCTGGGCTG AAGACCAAGG AGGCTGAACA GACCTGCCGC 
CACCTTACTG CCCAGGTGCG CAGCCTGGAG GCACAGGTTG CCCATGCAGA CCAGCAGCTT 
CGAGACCTGG GCAAATTCCA GGTGGCAACT GATGCTTTAA AGAGCCGTGA GCCCCAGGCT 
AAGCCCCAGC TGGACTTGAG TATTGACAGC CTGGATCTGA GCTGCGAGGA GGGGACCCCA 
CTCAGTATCA CCAGCAAGCT GCCTCGTACC CAGCCAGACG GCACCAGCGT CCCTGGAGAA 
CCAGCCTCAC CTATCTCCCA GCGCCTGCCC CCCAAGGTAG AATCCCTGGA GAGTCTCTAC 
TTCACTCCCA TCCCTGCTCG GAGTCAGGCC CCCCTGGAGA GCAGCCTGGA CTCCCTGGGA 
GACGTCTTCC TGGACTCGGG TCGTAAGACC CGCTCCGCTC GTCGGCGCAC CACGCAGATC 
ATCAACATCA CCATGACCAA GAAGCTAGAT GTGGAAGAGC CAGACAGCGC CAACTCATCG 
TTCTACAGCA CGCGGTCTGC TCCTGCTTCC CAGGCTAGCC TGCGAGCCAC CTCCTCTACT 
CAGTCTCTAG CTCGCCTGGG TTCTCCCGAT TATGGCAACT CAGCCCTGCT CAGCTTGCCT 
GGCTACCGCC CCACCACTCG CAGTTCTGCT CGTCGTTCCC AGGCCGGGGT GTCCAGTGGG 
GCCCCTCCAG GAAGGAACAG CTTCTACATG GGCACTTGCC AGGATGAGCC TGAGCAGCTG 
GATGACTGGA ACCGCATTGC AGAGCTGCAG CAGCGCAATC GAGTGTGCCC CCCACATCTG 
AAGACCTGCT ATCCCCTGGA GTCCAGGCCT TCCCTGAGCC TGGGCACCAT CACAGATGAG 
GAGATGAAAA CTGGAGACCC CCAAGAGACC CTGCGCCGAG CCAGCATGCA GCCAATCCAG 
ATAGCCGAGG GCACTGGCAT CACCACCCGG CAGCAGCGCA AACGGGTCTC CCTAGAGCCC 
CACCAGGGCC CTGGAACTCC TGAGTCTAAG AAGGCCACCA GCTGTTTCCC ACGCCCCATG 
ACTCCCCGAG ACCGACATGA AGGGCGCAAA CAGAGCACTA CTGAGGCCCA GAAGAAAGCA 
GCTCCAGCTT CTACTAAACA GGCTGACCGG CGCCAGTCGA TGGCCTTCAG CATCCTCAAC 
ACACCCAAGA AGCTAGGGAA CAGCCTTCTG CGGCGGGGAG CCTCAAAGAA GGCCCTGTCC 
AAGGCTTCCC CCAACACTCG CAGTGGAACC CGCCGTTCTC CGCGCATTGC CACCACCACA 
GCCAGCGCCG CCACTGCTGC CGCCATTGGT GCCACCCCTC GAGCCAAGGG CAAGGCAAAG 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873320.1
CDS	190..6495
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_017017831.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017831.1

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAAGAACTG 
AGTAAGAAAC TGGCTGACTC TGACCAAGCC AGCAAGGTGC AGCAGCAGAA GCTGAAGGCT 
GTCCAGGCTC AGGGAGGCGA GAGCCAGCAG GAGGCCCAGC GCCTCCAGGC CCAGCTGAAT 
GAACTGCAAG CCCAGTTGAG CCAGAAGGAG CAGGCAGCTG AGCACTATAA GCTGCAGATG 
GAGAAAGCCA AAACACATTA TGATGCCAAG AAGCAGCAGA ACCAAGAGCT GCAGGAGCAG 
CTGCGGAGCC TGGAGCAGCT GCAGAAGGAA AACAAAGAGC TGCGAGCTGA AGCTGAACGG 
CTGGGCCATG AGCTACAGCA GGCTGGGCTG AAGACCAAGG AGGCTGAACA GACCTGCCGC 
CACCTTACTG CCCAGGTGCG CAGCCTGGAG GCACAGGTTG CCCATGCAGA CCAGCAGCTT 
CGAGACCTGG GCAAATTCCA GGTGGCAACT GATGCTTTAA AGAGCCGTGA GCCCCAGGCT 
AAGCCCCAGC TGGACTTGAG TATTGACAGC CTGGATCTGA GCTGCGAGGA GGGGACCCCA 
CTCAGTATCA CCAGCAAGCT GCCTCGTACC CAGCCAGACG GCACCAGCGT CCCTGGAGAA 
CCAGCCTCAC CTATCTCCCA GCGCCTGCCC CCCAAGGTAG AATCCCTGGA GAGTCTCTAC 
TTCACTCCCA TCCCTGCTCG GAGTCAGGCC CCCCTGGAGA GCAGCCTGGA CTCCCTGGGA 
GACGTCTTCC TGGACTCGGG TCGTAAGACC CGCTCCGCTC GTCGGCGCAC CACGCAGATC 
ATCAACATCA CCATGACCAA GAAGCTAGAT GTGGAAGAGC CAGACAGCGC CAACTCATCG 
TTCTACAGCA CGCGGTCTGC TCCTGCTTCC CAGGCTAGCC TGCGAGCCAC CTCCTCTACT 
CAGTCTCTAG CTCGCCTGGG TTCTCCCGAT TATGGCAACT CAGCCCTGCT CAGCTTGCCT 
GGCTACCGCC CCACCACTCG CAGTTCTGCT CGTCGTTCCC AGGCCGGGGT GTCCAGTGGG 
GCCCCTCCAG GAAGGAACAG CTTCTACATG GGCACTTGCC AGGATGAGCC TGAGCAGCTG 
GATGACTGGA ACCGCATTGC AGAGCTGCAG CAGCGCAATC GAGTGTGCCC CCCACATCTG 
AAGACCTGCT ATCCCCTGGA GTCCAGGCCT TCCCTGAGCC TGGGCACCAT CACAGATGAG 
GAGATGAAAA CTGGAGACCC CCAAGAGACC CTGCGCCGAG CCAGCATGCA GCCAATCCAG 
ATAGCCGAGG GCACTGGCAT CACCACCCGG CAGCAGCGCA AACGGGTCTC CCTAGAGCCC 
CACCAGGGCC CTGGAACTCC TGAGTCTAAG AAGGCCACCA GCTGTTTCCC ACGCCCCATG 
ACTCCCCGAG ACCGACATGA AGGGCGCAAA CAGAGCACTA CTGAGGCCCA GAAGAAAGCA 
GCTCCAGCTT CTACTAAACA GGCTGACCGG CGCCAGTCGA TGGCCTTCAG CATCCTCAAC 
ACACCCAAGA AGCTAGGGAA CAGCCTTCTG CGGCGGGGAG CCTCAAAGAA GGCCCTGTCC 
AAGGCTTCCC CCAACACTCG CAGTGGAACC CGCCGTTCTC CGCGCATTGC CACCACCACA 
GCCAGCGCCG CCACTGCTGC CGCCATTGGT GCCACCCCTC GAGCCAAGGG CAAGGCAAAG 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545066.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543368.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X16, mRNA.

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CloneID	OHu23733
Clone ID Related Accession (Same CDS sequence)	XM_017017830.1 , XM_017017831.1 , XM_011545066.2 , XM_011545066.1 , NM_001286561.1
Accession Version	XM_011545066.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545066.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAAGAACTG 
AGTAAGAAAC TGGCTGACTC TGACCAAGCC AGCAAGGTGC AGCAGCAGAA GCTGAAGGCT 
GTCCAGGCTC AGGGAGGCGA GAGCCAGCAG GAGGCCCAGC GCCTCCAGGC CCAGCTGAAT 
GAACTGCAAG CCCAGTTGAG CCAGAAGGAG CAGGCAGCTG AGCACTATAA GCTGCAGATG 
GAGAAAGCCA AAACACATTA TGATGCCAAG AAGCAGCAGA ACCAAGAGCT GCAGGAGCAG 
CTGCGGAGCC TGGAGCAGCT GCAGAAGGAA AACAAAGAGC TGCGAGCTGA AGCTGAACGG 
CTGGGCCATG AGCTACAGCA GGCTGGGCTG AAGACCAAGG AGGCTGAACA GACCTGCCGC 
CACCTTACTG CCCAGGTGCG CAGCCTGGAG GCACAGGTTG CCCATGCAGA CCAGCAGCTT 
CGAGACCTGG GCAAATTCCA GGTGGCAACT GATGCTTTAA AGAGCCGTGA GCCCCAGGCT 
AAGCCCCAGC TGGACTTGAG TATTGACAGC CTGGATCTGA GCTGCGAGGA GGGGACCCCA 
CTCAGTATCA CCAGCAAGCT GCCTCGTACC CAGCCAGACG GCACCAGCGT CCCTGGAGAA 
CCAGCCTCAC CTATCTCCCA GCGCCTGCCC CCCAAGGTAG AATCCCTGGA GAGTCTCTAC 
TTCACTCCCA TCCCTGCTCG GAGTCAGGCC CCCCTGGAGA GCAGCCTGGA CTCCCTGGGA 
GACGTCTTCC TGGACTCGGG TCGTAAGACC CGCTCCGCTC GTCGGCGCAC CACGCAGATC 
ATCAACATCA CCATGACCAA GAAGCTAGAT GTGGAAGAGC CAGACAGCGC CAACTCATCG 
TTCTACAGCA CGCGGTCTGC TCCTGCTTCC CAGGCTAGCC TGCGAGCCAC CTCCTCTACT 
CAGTCTCTAG CTCGCCTGGG TTCTCCCGAT TATGGCAACT CAGCCCTGCT CAGCTTGCCT 
GGCTACCGCC CCACCACTCG CAGTTCTGCT CGTCGTTCCC AGGCCGGGGT GTCCAGTGGG 
GCCCCTCCAG GAAGGAACAG CTTCTACATG GGCACTTGCC AGGATGAGCC TGAGCAGCTG 
GATGACTGGA ACCGCATTGC AGAGCTGCAG CAGCGCAATC GAGTGTGCCC CCCACATCTG 
AAGACCTGCT ATCCCCTGGA GTCCAGGCCT TCCCTGAGCC TGGGCACCAT CACAGATGAG 
GAGATGAAAA CTGGAGACCC CCAAGAGACC CTGCGCCGAG CCAGCATGCA GCCAATCCAG 
ATAGCCGAGG GCACTGGCAT CACCACCCGG CAGCAGCGCA AACGGGTCTC CCTAGAGCCC 
CACCAGGGCC CTGGAACTCC TGAGTCTAAG AAGGCCACCA GCTGTTTCCC ACGCCCCATG 
ACTCCCCGAG ACCGACATGA AGGGCGCAAA CAGAGCACTA CTGAGGCCCA GAAGAAAGCA 
GCTCCAGCTT CTACTAAACA GGCTGACCGG CGCCAGTCGA TGGCCTTCAG CATCCTCAAC 
ACACCCAAGA AGCTAGGGAA CAGCCTTCTG CGGCGGGGAG CCTCAAAGAA GGCCCTGTCC 
AAGGCTTCCC CCAACACTCG CAGTGGAACC CGCCGTTCTC CGCGCATTGC CACCACCACA 
GCCAGCGCCG CCACTGCTGC CGCCATTGGT GCCACCCCTC GAGCCAAGGG CAAGGCAAAG 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543368.1
CDS	161..6466
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545066.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545066.2

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAAGAACTG 
AGTAAGAAAC TGGCTGACTC TGACCAAGCC AGCAAGGTGC AGCAGCAGAA GCTGAAGGCT 
GTCCAGGCTC AGGGAGGCGA GAGCCAGCAG GAGGCCCAGC GCCTCCAGGC CCAGCTGAAT 
GAACTGCAAG CCCAGTTGAG CCAGAAGGAG CAGGCAGCTG AGCACTATAA GCTGCAGATG 
GAGAAAGCCA AAACACATTA TGATGCCAAG AAGCAGCAGA ACCAAGAGCT GCAGGAGCAG 
CTGCGGAGCC TGGAGCAGCT GCAGAAGGAA AACAAAGAGC TGCGAGCTGA AGCTGAACGG 
CTGGGCCATG AGCTACAGCA GGCTGGGCTG AAGACCAAGG AGGCTGAACA GACCTGCCGC 
CACCTTACTG CCCAGGTGCG CAGCCTGGAG GCACAGGTTG CCCATGCAGA CCAGCAGCTT 
CGAGACCTGG GCAAATTCCA GGTGGCAACT GATGCTTTAA AGAGCCGTGA GCCCCAGGCT 
AAGCCCCAGC TGGACTTGAG TATTGACAGC CTGGATCTGA GCTGCGAGGA GGGGACCCCA 
CTCAGTATCA CCAGCAAGCT GCCTCGTACC CAGCCAGACG GCACCAGCGT CCCTGGAGAA 
CCAGCCTCAC CTATCTCCCA GCGCCTGCCC CCCAAGGTAG AATCCCTGGA GAGTCTCTAC 
TTCACTCCCA TCCCTGCTCG GAGTCAGGCC CCCCTGGAGA GCAGCCTGGA CTCCCTGGGA 
GACGTCTTCC TGGACTCGGG TCGTAAGACC CGCTCCGCTC GTCGGCGCAC CACGCAGATC 
ATCAACATCA CCATGACCAA GAAGCTAGAT GTGGAAGAGC CAGACAGCGC CAACTCATCG 
TTCTACAGCA CGCGGTCTGC TCCTGCTTCC CAGGCTAGCC TGCGAGCCAC CTCCTCTACT 
CAGTCTCTAG CTCGCCTGGG TTCTCCCGAT TATGGCAACT CAGCCCTGCT CAGCTTGCCT 
GGCTACCGCC CCACCACTCG CAGTTCTGCT CGTCGTTCCC AGGCCGGGGT GTCCAGTGGG 
GCCCCTCCAG GAAGGAACAG CTTCTACATG GGCACTTGCC AGGATGAGCC TGAGCAGCTG 
GATGACTGGA ACCGCATTGC AGAGCTGCAG CAGCGCAATC GAGTGTGCCC CCCACATCTG 
AAGACCTGCT ATCCCCTGGA GTCCAGGCCT TCCCTGAGCC TGGGCACCAT CACAGATGAG 
GAGATGAAAA CTGGAGACCC CCAAGAGACC CTGCGCCGAG CCAGCATGCA GCCAATCCAG 
ATAGCCGAGG GCACTGGCAT CACCACCCGG CAGCAGCGCA AACGGGTCTC CCTAGAGCCC 
CACCAGGGCC CTGGAACTCC TGAGTCTAAG AAGGCCACCA GCTGTTTCCC ACGCCCCATG 
ACTCCCCGAG ACCGACATGA AGGGCGCAAA CAGAGCACTA CTGAGGCCCA GAAGAAAGCA 
GCTCCAGCTT CTACTAAACA GGCTGACCGG CGCCAGTCGA TGGCCTTCAG CATCCTCAAC 
ACACCCAAGA AGCTAGGGAA CAGCCTTCTG CGGCGGGGAG CCTCAAAGAA GGCCCTGTCC 
AAGGCTTCCC CCAACACTCG CAGTGGAACC CGCCGTTCTC CGCGCATTGC CACCACCACA 
GCCAGCGCCG CCACTGCTGC CGCCATTGGT GCCACCCCTC GAGCCAAGGG CAAGGCAAAG 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545066.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543368.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X14, mRNA.

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CloneID	OHu23733
Clone ID Related Accession (Same CDS sequence)	XM_017017830.1 , XM_017017831.1 , XM_011545066.2 , XM_011545066.1 , NM_001286561.1
Accession Version	XM_011545066.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAAGAACTG 
AGTAAGAAAC TGGCTGACTC TGACCAAGCC AGCAAGGTGC AGCAGCAGAA GCTGAAGGCT 
GTCCAGGCTC AGGGAGGCGA GAGCCAGCAG GAGGCCCAGC GCCTCCAGGC CCAGCTGAAT 
GAACTGCAAG CCCAGTTGAG CCAGAAGGAG CAGGCAGCTG AGCACTATAA GCTGCAGATG 
GAGAAAGCCA AAACACATTA TGATGCCAAG AAGCAGCAGA ACCAAGAGCT GCAGGAGCAG 
CTGCGGAGCC TGGAGCAGCT GCAGAAGGAA AACAAAGAGC TGCGAGCTGA AGCTGAACGG 
CTGGGCCATG AGCTACAGCA GGCTGGGCTG AAGACCAAGG AGGCTGAACA GACCTGCCGC 
CACCTTACTG CCCAGGTGCG CAGCCTGGAG GCACAGGTTG CCCATGCAGA CCAGCAGCTT 
CGAGACCTGG GCAAATTCCA GGTGGCAACT GATGCTTTAA AGAGCCGTGA GCCCCAGGCT 
AAGCCCCAGC TGGACTTGAG TATTGACAGC CTGGATCTGA GCTGCGAGGA GGGGACCCCA 
CTCAGTATCA CCAGCAAGCT GCCTCGTACC CAGCCAGACG GCACCAGCGT CCCTGGAGAA 
CCAGCCTCAC CTATCTCCCA GCGCCTGCCC CCCAAGGTAG AATCCCTGGA GAGTCTCTAC 
TTCACTCCCA TCCCTGCTCG GAGTCAGGCC CCCCTGGAGA GCAGCCTGGA CTCCCTGGGA 
GACGTCTTCC TGGACTCGGG TCGTAAGACC CGCTCCGCTC GTCGGCGCAC CACGCAGATC 
ATCAACATCA CCATGACCAA GAAGCTAGAT GTGGAAGAGC CAGACAGCGC CAACTCATCG 
TTCTACAGCA CGCGGTCTGC TCCTGCTTCC CAGGCTAGCC TGCGAGCCAC CTCCTCTACT 
CAGTCTCTAG CTCGCCTGGG TTCTCCCGAT TATGGCAACT CAGCCCTGCT CAGCTTGCCT 
GGCTACCGCC CCACCACTCG CAGTTCTGCT CGTCGTTCCC AGGCCGGGGT GTCCAGTGGG 
GCCCCTCCAG GAAGGAACAG CTTCTACATG GGCACTTGCC AGGATGAGCC TGAGCAGCTG 
GATGACTGGA ACCGCATTGC AGAGCTGCAG CAGCGCAATC GAGTGTGCCC CCCACATCTG 
AAGACCTGCT ATCCCCTGGA GTCCAGGCCT TCCCTGAGCC TGGGCACCAT CACAGATGAG 
GAGATGAAAA CTGGAGACCC CCAAGAGACC CTGCGCCGAG CCAGCATGCA GCCAATCCAG 
ATAGCCGAGG GCACTGGCAT CACCACCCGG CAGCAGCGCA AACGGGTCTC CCTAGAGCCC 
CACCAGGGCC CTGGAACTCC TGAGTCTAAG AAGGCCACCA GCTGTTTCCC ACGCCCCATG 
ACTCCCCGAG ACCGACATGA AGGGCGCAAA CAGAGCACTA CTGAGGCCCA GAAGAAAGCA 
GCTCCAGCTT CTACTAAACA GGCTGACCGG CGCCAGTCGA TGGCCTTCAG CATCCTCAAC 
ACACCCAAGA AGCTAGGGAA CAGCCTTCTG CGGCGGGGAG CCTCAAAGAA GGCCCTGTCC 
AAGGCTTCCC CCAACACTCG CAGTGGAACC CGCCGTTCTC CGCGCATTGC CACCACCACA 
GCCAGCGCCG CCACTGCTGC CGCCATTGGT GCCACCCCTC GAGCCAAGGG CAAGGCAAAG 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543368.1
CDS	154..6459
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545066.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545066.1

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAAGAACTG 
AGTAAGAAAC TGGCTGACTC TGACCAAGCC AGCAAGGTGC AGCAGCAGAA GCTGAAGGCT 
GTCCAGGCTC AGGGAGGCGA GAGCCAGCAG GAGGCCCAGC GCCTCCAGGC CCAGCTGAAT 
GAACTGCAAG CCCAGTTGAG CCAGAAGGAG CAGGCAGCTG AGCACTATAA GCTGCAGATG 
GAGAAAGCCA AAACACATTA TGATGCCAAG AAGCAGCAGA ACCAAGAGCT GCAGGAGCAG 
CTGCGGAGCC TGGAGCAGCT GCAGAAGGAA AACAAAGAGC TGCGAGCTGA AGCTGAACGG 
CTGGGCCATG AGCTACAGCA GGCTGGGCTG AAGACCAAGG AGGCTGAACA GACCTGCCGC 
CACCTTACTG CCCAGGTGCG CAGCCTGGAG GCACAGGTTG CCCATGCAGA CCAGCAGCTT 
CGAGACCTGG GCAAATTCCA GGTGGCAACT GATGCTTTAA AGAGCCGTGA GCCCCAGGCT 
AAGCCCCAGC TGGACTTGAG TATTGACAGC CTGGATCTGA GCTGCGAGGA GGGGACCCCA 
CTCAGTATCA CCAGCAAGCT GCCTCGTACC CAGCCAGACG GCACCAGCGT CCCTGGAGAA 
CCAGCCTCAC CTATCTCCCA GCGCCTGCCC CCCAAGGTAG AATCCCTGGA GAGTCTCTAC 
TTCACTCCCA TCCCTGCTCG GAGTCAGGCC CCCCTGGAGA GCAGCCTGGA CTCCCTGGGA 
GACGTCTTCC TGGACTCGGG TCGTAAGACC CGCTCCGCTC GTCGGCGCAC CACGCAGATC 
ATCAACATCA CCATGACCAA GAAGCTAGAT GTGGAAGAGC CAGACAGCGC CAACTCATCG 
TTCTACAGCA CGCGGTCTGC TCCTGCTTCC CAGGCTAGCC TGCGAGCCAC CTCCTCTACT 
CAGTCTCTAG CTCGCCTGGG TTCTCCCGAT TATGGCAACT CAGCCCTGCT CAGCTTGCCT 
GGCTACCGCC CCACCACTCG CAGTTCTGCT CGTCGTTCCC AGGCCGGGGT GTCCAGTGGG 
GCCCCTCCAG GAAGGAACAG CTTCTACATG GGCACTTGCC AGGATGAGCC TGAGCAGCTG 
GATGACTGGA ACCGCATTGC AGAGCTGCAG CAGCGCAATC GAGTGTGCCC CCCACATCTG 
AAGACCTGCT ATCCCCTGGA GTCCAGGCCT TCCCTGAGCC TGGGCACCAT CACAGATGAG 
GAGATGAAAA CTGGAGACCC CCAAGAGACC CTGCGCCGAG CCAGCATGCA GCCAATCCAG 
ATAGCCGAGG GCACTGGCAT CACCACCCGG CAGCAGCGCA AACGGGTCTC CCTAGAGCCC 
CACCAGGGCC CTGGAACTCC TGAGTCTAAG AAGGCCACCA GCTGTTTCCC ACGCCCCATG 
ACTCCCCGAG ACCGACATGA AGGGCGCAAA CAGAGCACTA CTGAGGCCCA GAAGAAAGCA 
GCTCCAGCTT CTACTAAACA GGCTGACCGG CGCCAGTCGA TGGCCTTCAG CATCCTCAAC 
ACACCCAAGA AGCTAGGGAA CAGCCTTCTG CGGCGGGGAG CCTCAAAGAA GGCCCTGTCC 
AAGGCTTCCC CCAACACTCG CAGTGGAACC CGCCGTTCTC CGCGCATTGC CACCACCACA 
GCCAGCGCCG CCACTGCTGC CGCCATTGGT GCCACCCCTC GAGCCAAGGG CAAGGCAAAG 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545054.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543356.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA.

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CloneID	OHu59363
Clone ID Related Accession (Same CDS sequence)	XM_011545054.1 , XM_011545057.2 , XM_024448555.1 , XM_011545059.2 , XM_011545060.2 , XM_011545064.1 , XM_011545055.1 , XM_011545056.2 , XM_011545058.1 , XM_011545063.2 , XM_011545062.2 , XM_011545061.2 , XM_011545054.2 , XM_011545063.3
Accession Version	XM_011545054.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543356.1
CDS	154..6555
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITRHKALMTIIPDLSPNNPLSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545054.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545054.1

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545057.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543359.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X4, mRNA.

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CloneID	OHu59363
Clone ID Related Accession (Same CDS sequence)	XM_011545054.1 , XM_011545057.2 , XM_024448555.1 , XM_011545059.2 , XM_011545060.2 , XM_011545064.1 , XM_011545055.1 , XM_011545056.2 , XM_011545058.1 , XM_011545063.2 , XM_011545062.2 , XM_011545061.2 , XM_011545054.2 , XM_011545063.3
Accession Version	XM_011545057.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543359.1
CDS	316..6717
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITRHKALMTIIPDLSPNNPLSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545057.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545057.2

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_024448555.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304323.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X5, mRNA.

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CloneID	OHu59363
Clone ID Related Accession (Same CDS sequence)	XM_011545054.1 , XM_011545057.2 , XM_024448555.1 , XM_011545059.2 , XM_011545060.2 , XM_011545064.1 , XM_011545055.1 , XM_011545056.2 , XM_011545058.1 , XM_011545063.2 , XM_011545062.2 , XM_011545061.2 , XM_011545054.2 , XM_011545063.3
Accession Version	XM_024448555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304323.1
CDS	258..6659
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITRHKALMTIIPDLSPNNPLSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_024448555.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448555.1

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545059.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543361.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X7, mRNA.

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CloneID	OHu59363
Clone ID Related Accession (Same CDS sequence)	XM_011545054.1 , XM_011545057.2 , XM_024448555.1 , XM_011545059.2 , XM_011545060.2 , XM_011545064.1 , XM_011545055.1 , XM_011545056.2 , XM_011545058.1 , XM_011545063.2 , XM_011545062.2 , XM_011545061.2 , XM_011545054.2 , XM_011545063.3
Accession Version	XM_011545059.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545059.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543361.1
CDS	318..6719
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITRHKALMTIIPDLSPNNPLSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545059.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545059.2

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545060.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543362.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X9, mRNA.

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CloneID	OHu59363
Clone ID Related Accession (Same CDS sequence)	XM_011545054.1 , XM_011545057.2 , XM_024448555.1 , XM_011545059.2 , XM_011545060.2 , XM_011545064.1 , XM_011545055.1 , XM_011545056.2 , XM_011545058.1 , XM_011545063.2 , XM_011545062.2 , XM_011545061.2 , XM_011545054.2 , XM_011545063.3
Accession Version	XM_011545060.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545060.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543362.1
CDS	312..6713
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITRHKALMTIIPDLSPNNPLSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545060.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545060.2

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545064.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543366.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X12, mRNA.

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CloneID	OHu59363
Clone ID Related Accession (Same CDS sequence)	XM_011545054.1 , XM_011545057.2 , XM_024448555.1 , XM_011545059.2 , XM_011545060.2 , XM_011545064.1 , XM_011545055.1 , XM_011545056.2 , XM_011545058.1 , XM_011545063.2 , XM_011545062.2 , XM_011545061.2 , XM_011545054.2 , XM_011545063.3
Accession Version	XM_011545064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543366.1
CDS	139..6540
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITRHKALMTIIPDLSPNNPLSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545064.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545064.1

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545055.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543357.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X2, mRNA.

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CloneID	OHu59363
Clone ID Related Accession (Same CDS sequence)	XM_011545054.1 , XM_011545057.2 , XM_024448555.1 , XM_011545059.2 , XM_011545060.2 , XM_011545064.1 , XM_011545055.1 , XM_011545056.2 , XM_011545058.1 , XM_011545063.2 , XM_011545062.2 , XM_011545061.2 , XM_011545054.2 , XM_011545063.3
Accession Version	XM_011545055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543357.1
CDS	332..6733
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITRHKALMTIIPDLSPNNPLSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545055.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545055.1

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545056.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543358.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X3, mRNA.

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CloneID	OHu59363
Clone ID Related Accession (Same CDS sequence)	XM_011545054.1 , XM_011545057.2 , XM_024448555.1 , XM_011545059.2 , XM_011545060.2 , XM_011545064.1 , XM_011545055.1 , XM_011545056.2 , XM_011545058.1 , XM_011545063.2 , XM_011545062.2 , XM_011545061.2 , XM_011545054.2 , XM_011545063.3
Accession Version	XM_011545056.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545056.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543358.1
CDS	326..6727
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITRHKALMTIIPDLSPNNPLSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545056.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545056.2

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545058.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543360.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X6, mRNA.

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CloneID	OHu59363
Clone ID Related Accession (Same CDS sequence)	XM_011545054.1 , XM_011545057.2 , XM_024448555.1 , XM_011545059.2 , XM_011545060.2 , XM_011545064.1 , XM_011545055.1 , XM_011545056.2 , XM_011545058.1 , XM_011545063.2 , XM_011545062.2 , XM_011545061.2 , XM_011545054.2 , XM_011545063.3
Accession Version	XM_011545058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543360.1
CDS	353..6754
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITRHKALMTIIPDLSPNNPLSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545058.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545058.1

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545063.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543365.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X10, mRNA.

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CloneID	OHu59363
Clone ID Related Accession (Same CDS sequence)	XM_011545054.1 , XM_011545057.2 , XM_024448555.1 , XM_011545059.2 , XM_011545060.2 , XM_011545064.1 , XM_011545055.1 , XM_011545056.2 , XM_011545058.1 , XM_011545063.2 , XM_011545062.2 , XM_011545061.2 , XM_011545054.2 , XM_011545063.3
Accession Version	XM_011545063.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545063.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543365.1
CDS	152..6553
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITRHKALMTIIPDLSPNNPLSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545063.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545063.2

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545062.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543364.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X10, mRNA.

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CloneID	OHu59363
Clone ID Related Accession (Same CDS sequence)	XM_011545054.1 , XM_011545057.2 , XM_024448555.1 , XM_011545059.2 , XM_011545060.2 , XM_011545064.1 , XM_011545055.1 , XM_011545056.2 , XM_011545058.1 , XM_011545063.2 , XM_011545062.2 , XM_011545061.2 , XM_011545054.2 , XM_011545063.3
Accession Version	XM_011545062.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545062.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543364.1
CDS	185..6586
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITRHKALMTIIPDLSPNNPLSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545062.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545062.2

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545061.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543363.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X8, mRNA.

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CloneID	OHu59363
Clone ID Related Accession (Same CDS sequence)	XM_011545054.1 , XM_011545057.2 , XM_024448555.1 , XM_011545059.2 , XM_011545060.2 , XM_011545064.1 , XM_011545055.1 , XM_011545056.2 , XM_011545058.1 , XM_011545063.2 , XM_011545062.2 , XM_011545061.2 , XM_011545054.2 , XM_011545063.3
Accession Version	XM_011545061.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545061.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543363.1
CDS	172..6573
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITRHKALMTIIPDLSPNNPLSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545061.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545061.2

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545054.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543356.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA.

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CloneID	OHu59363
Clone ID Related Accession (Same CDS sequence)	XM_011545054.1 , XM_011545057.2 , XM_024448555.1 , XM_011545059.2 , XM_011545060.2 , XM_011545064.1 , XM_011545055.1 , XM_011545056.2 , XM_011545058.1 , XM_011545063.2 , XM_011545062.2 , XM_011545061.2 , XM_011545054.2 , XM_011545063.3
Accession Version	XM_011545054.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545054.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543356.1
CDS	200..6601
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITRHKALMTIIPDLSPNNPLSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545054.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545054.2

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545063.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543365.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X11, mRNA.

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CloneID	OHu59363
Clone ID Related Accession (Same CDS sequence)	XM_011545054.1 , XM_011545057.2 , XM_024448555.1 , XM_011545059.2 , XM_011545060.2 , XM_011545064.1 , XM_011545055.1 , XM_011545056.2 , XM_011545058.1 , XM_011545063.2 , XM_011545062.2 , XM_011545061.2 , XM_011545054.2 , XM_011545063.3
Accession Version	XM_011545063.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545063.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543365.1
CDS	107..6508
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITRHKALMTIIPDLSPNNPLSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545063.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545063.3

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAGCAGCTA 
GAGGTATTTC AGAGAGAGCA AACTAAGCAG GTGGAAGAAC TGAGTAAGAA ACTGGCTGAC 
TCTGACCAAG CCAGCAAGGT GCAGCAGCAG AAGCTGAAGG CTGTCCAGGC TCAGGGAGGC 
GAGAGCCAGC AGGAGGCCCA GCGCCTCCAG GCCCAGCTGA ATGAACTGCA AGCCCAGTTG 
AGCCAGAAGG AGCAGGCAGC TGAGCACTAT AAGCTGCAGA TGGAGAAAGC CAAAACACAT 
TATGATGCCA AGAAGCAGCA GAACCAAGAG CTGCAGGAGC AGCTGCGGAG CCTGGAGCAG 
CTGCAGAAGG AAAACAAAGA GCTGCGAGCT GAAGCTGAAC GGCTGGGCCA TGAGCTACAG 
CAGGCTGGGC TGAAGACCAA GGAGGCTGAA CAGACCTGCC GCCACCTTAC TGCCCAGGTG 
CGCAGCCTGG AGGCACAGGT TGCCCATGCA GACCAGCAGC TTCGAGACCT GGGCAAATTC 
CAGGTGGCAA CTGATGCTTT AAAGAGCCGT GAGCCCCAGG CTAAGCCCCA GCTGGACTTG 
AGTATTGACA GCCTGGATCT GAGCTGCGAG GAGGGGACCC CACTCAGTAT CACCAGACAT 
AAAGCACTCA TGACAATTAT ACCTGATTTG TCCCCCAACA ACCCTCTGAG CAAGCTGCCT 
CGTACCCAGC CAGACGGCAC CAGCGTCCCT GGAGAACCAG CCTCACCTAT CTCCCAGCGC 
CTGCCCCCCA AGGTAGAATC CCTGGAGAGT CTCTACTTCA CTCCCATCCC TGCTCGGAGT 
CAGGCCCCCC TGGAGAGCAG CCTGGACTCC CTGGGAGACG TCTTCCTGGA CTCGGGTCGT 
AAGACCCGCT CCGCTCGTCG GCGCACCACG CAGATCATCA ACATCACCAT GACCAAGAAG 
CTAGATGTGG AAGAGCCAGA CAGCGCCAAC TCATCGTTCT ACAGCACGCG GTCTGCTCCT 
GCTTCCCAGG CTAGCCTGCG AGCCACCTCC TCTACTCAGT CTCTAGCTCG CCTGGGTTCT 
CCCGATTATG GCAACTCAGC CCTGCTCAGC TTGCCTGGCT ACCGCCCCAC CACTCGCAGT 
TCTGCTCGTC GTTCCCAGGC CGGGGTGTCC AGTGGGGCCC CTCCAGGAAG GAACAGCTTC 
TACATGGGCA CTTGCCAGGA TGAGCCTGAG CAGCTGGATG ACTGGAACCG CATTGCAGAG 
CTGCAGCAGC GCAATCGAGT GTGCCCCCCA CATCTGAAGA CCTGCTATCC CCTGGAGTCC 
AGGCCTTCCC TGAGCCTGGG CACCATCACA GATGAGGAGA TGAAAACTGG AGACCCCCAA 
GAGACCCTGC GCCGAGCCAG CATGCAGCCA ATCCAGATAG CCGAGGGCAC TGGCATCACC 
ACCCGGCAGC AGCGCAAACG GGTCTCCCTA GAGCCCCACC AGGGCCCTGG AACTCCTGAG 
TCTAAGAAGG CCACCAGCTG TTTCCCACGC CCCATGACTC CCCGAGACCG ACATGAAGGG 
CGCAAACAGA GCACTACTGA GGCCCAGAAG AAAGCAGCTC CAGCTTCTAC TAAACAGGCT 
GACCGGCGCC AGTCGATGGC CTTCAGCATC CTCAACACAC CCAAGAAGCT AGGGAACAGC 
CTTCTGCGGC GGGGAGCCTC AAAGAAGGCC CTGTCCAAGG CTTCCCCCAA CACTCGCAGT 
GGAACCCGCC GTTCTCCGCG CATTGCCACC ACCACAGCCA GCGCCGCCAC TGCTGCCGCC 
ATTGGTGCCA CCCCTCGAGC CAAGGGCAAG GCAAAGCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545065.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543367.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X12, mRNA.

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CloneID	OHu59364
Clone ID Related Accession (Same CDS sequence)	XM_011545065.1 , XM_011545065.2
Accession Version	XM_011545065.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAAGAACTG 
AGTAAGAAAC TGGCTGACTC TGACCAAGCC AGCAAGGTGC AGCAGCAGAA GCTGAAGGCT 
GTCCAGGCTC AGGGAGGCGA GAGCCAGCAG GAGGCCCAGC GCCTCCAGGC CCAGCTGAAT 
GAACTGCAAG CCCAGTTGAG CCAGAAGGAG CAGGCAGCTG AGCACTATAA GCTGCAGATG 
GAGAAAGCCA AAACACATTA TGATGCCAAG AAGCAGCAGA ACCAAGAGCT GCAGGAGCAG 
CTGCGGAGCC TGGAGCAGCT GCAGAAGGAA AACAAAGAGC TGCGAGCTGA AGCTGAACGG 
CTGGGCCATG AGCTACAGCA GGCTGGGCTG AAGACCAAGG AGGCTGAACA GACCTGCCGC 
CACCTTACTG CCCAGGTGCG CAGCCTGGAG GCACAGGTTG CCCATGCAGA CCAGCAGCTT 
CGAGACCTGG GCAAATTCCA GGTGGCAACT GATGCTTTAA AGAGCCGTGA GCCCCAGGCT 
AAGCCCCAGC TGGACTTGAG TATTGACAGC CTGGATCTGA GCTGCGAGGA GGGGACCCCA 
CTCAGTATCA CCAGACATAA AGCACTCATG ACAATTATAC CTGATTTGTC CCCCAACAAC 
CCTCTGAGCA AGCTGCCTCG TACCCAGCCA GACGGCACCA GCGTCCCTGG AGAACCAGCC 
TCACCTATCT CCCAGCGCCT GCCCCCCAAG GTAGAATCCC TGGAGAGTCT CTACTTCACT 
CCCATCCCTG CTCGGAGTCA GGCCCCCCTG GAGAGCAGCC TGGACTCCCT GGGAGACGTC 
TTCCTGGACT CGGGTCGTAA GACCCGCTCC GCTCGTCGGC GCACCACGCA GATCATCAAC 
ATCACCATGA CCAAGAAGCT AGATGTGGAA GAGCCAGACA GCGCCAACTC ATCGTTCTAC 
AGCACGCGGT CTGCTCCTGC TTCCCAGGCT AGCCTGCGAG CCACCTCCTC TACTCAGTCT 
CTAGCTCGCC TGGGTTCTCC CGATTATGGC AACTCAGCCC TGCTCAGCTT GCCTGGCTAC 
CGCCCCACCA CTCGCAGTTC TGCTCGTCGT TCCCAGGCCG GGGTGTCCAG TGGGGCCCCT 
CCAGGAAGGA ACAGCTTCTA CATGGGCACT TGCCAGGATG AGCCTGAGCA GCTGGATGAC 
TGGAACCGCA TTGCAGAGCT GCAGCAGCGC AATCGAGTGT GCCCCCCACA TCTGAAGACC 
TGCTATCCCC TGGAGTCCAG GCCTTCCCTG AGCCTGGGCA CCATCACAGA TGAGGAGATG 
AAAACTGGAG ACCCCCAAGA GACCCTGCGC CGAGCCAGCA TGCAGCCAAT CCAGATAGCC 
GAGGGCACTG GCATCACCAC CCGGCAGCAG CGCAAACGGG TCTCCCTAGA GCCCCACCAG 
GGCCCTGGAA CTCCTGAGTC TAAGAAGGCC ACCAGCTGTT TCCCACGCCC CATGACTCCC 
CGAGACCGAC ATGAAGGGCG CAAACAGAGC ACTACTGAGG CCCAGAAGAA AGCAGCTCCA 
GCTTCTACTA AACAGGCTGA CCGGCGCCAG TCGATGGCCT TCAGCATCCT CAACACACCC 
AAGAAGCTAG GGAACAGCCT TCTGCGGCGG GGAGCCTCAA AGAAGGCCCT GTCCAAGGCT 
TCCCCCAACA CTCGCAGTGG AACCCGCCGT TCTCCGCGCA TTGCCACCAC CACAGCCAGC 
GCCGCCACTG CTGCCGCCAT TGGTGCCACC CCTCGAGCCA AGGGCAAGGC AAAGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543367.1
CDS	154..6513
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITRHKALMTIIPDLSPNNPLSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545065.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545065.1

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAAGAACTG 
AGTAAGAAAC TGGCTGACTC TGACCAAGCC AGCAAGGTGC AGCAGCAGAA GCTGAAGGCT 
GTCCAGGCTC AGGGAGGCGA GAGCCAGCAG GAGGCCCAGC GCCTCCAGGC CCAGCTGAAT 
GAACTGCAAG CCCAGTTGAG CCAGAAGGAG CAGGCAGCTG AGCACTATAA GCTGCAGATG 
GAGAAAGCCA AAACACATTA TGATGCCAAG AAGCAGCAGA ACCAAGAGCT GCAGGAGCAG 
CTGCGGAGCC TGGAGCAGCT GCAGAAGGAA AACAAAGAGC TGCGAGCTGA AGCTGAACGG 
CTGGGCCATG AGCTACAGCA GGCTGGGCTG AAGACCAAGG AGGCTGAACA GACCTGCCGC 
CACCTTACTG CCCAGGTGCG CAGCCTGGAG GCACAGGTTG CCCATGCAGA CCAGCAGCTT 
CGAGACCTGG GCAAATTCCA GGTGGCAACT GATGCTTTAA AGAGCCGTGA GCCCCAGGCT 
AAGCCCCAGC TGGACTTGAG TATTGACAGC CTGGATCTGA GCTGCGAGGA GGGGACCCCA 
CTCAGTATCA CCAGACATAA AGCACTCATG ACAATTATAC CTGATTTGTC CCCCAACAAC 
CCTCTGAGCA AGCTGCCTCG TACCCAGCCA GACGGCACCA GCGTCCCTGG AGAACCAGCC 
TCACCTATCT CCCAGCGCCT GCCCCCCAAG GTAGAATCCC TGGAGAGTCT CTACTTCACT 
CCCATCCCTG CTCGGAGTCA GGCCCCCCTG GAGAGCAGCC TGGACTCCCT GGGAGACGTC 
TTCCTGGACT CGGGTCGTAA GACCCGCTCC GCTCGTCGGC GCACCACGCA GATCATCAAC 
ATCACCATGA CCAAGAAGCT AGATGTGGAA GAGCCAGACA GCGCCAACTC ATCGTTCTAC 
AGCACGCGGT CTGCTCCTGC TTCCCAGGCT AGCCTGCGAG CCACCTCCTC TACTCAGTCT 
CTAGCTCGCC TGGGTTCTCC CGATTATGGC AACTCAGCCC TGCTCAGCTT GCCTGGCTAC 
CGCCCCACCA CTCGCAGTTC TGCTCGTCGT TCCCAGGCCG GGGTGTCCAG TGGGGCCCCT 
CCAGGAAGGA ACAGCTTCTA CATGGGCACT TGCCAGGATG AGCCTGAGCA GCTGGATGAC 
TGGAACCGCA TTGCAGAGCT GCAGCAGCGC AATCGAGTGT GCCCCCCACA TCTGAAGACC 
TGCTATCCCC TGGAGTCCAG GCCTTCCCTG AGCCTGGGCA CCATCACAGA TGAGGAGATG 
AAAACTGGAG ACCCCCAAGA GACCCTGCGC CGAGCCAGCA TGCAGCCAAT CCAGATAGCC 
GAGGGCACTG GCATCACCAC CCGGCAGCAG CGCAAACGGG TCTCCCTAGA GCCCCACCAG 
GGCCCTGGAA CTCCTGAGTC TAAGAAGGCC ACCAGCTGTT TCCCACGCCC CATGACTCCC 
CGAGACCGAC ATGAAGGGCG CAAACAGAGC ACTACTGAGG CCCAGAAGAA AGCAGCTCCA 
GCTTCTACTA AACAGGCTGA CCGGCGCCAG TCGATGGCCT TCAGCATCCT CAACACACCC 
AAGAAGCTAG GGAACAGCCT TCTGCGGCGG GGAGCCTCAA AGAAGGCCCT GTCCAAGGCT 
TCCCCCAACA CTCGCAGTGG AACCCGCCGT TCTCCGCGCA TTGCCACCAC CACAGCCAGC 
GCCGCCACTG CTGCCGCCAT TGGTGCCACC CCTCGAGCCA AGGGCAAGGC AAAGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_011545065.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543367.1 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X13, mRNA.

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CloneID	OHu59364
Clone ID Related Accession (Same CDS sequence)	XM_011545065.1 , XM_011545065.2
Accession Version	XM_011545065.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear mitotic apparatus protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545065.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAAGAACTG 
AGTAAGAAAC TGGCTGACTC TGACCAAGCC AGCAAGGTGC AGCAGCAGAA GCTGAAGGCT 
GTCCAGGCTC AGGGAGGCGA GAGCCAGCAG GAGGCCCAGC GCCTCCAGGC CCAGCTGAAT 
GAACTGCAAG CCCAGTTGAG CCAGAAGGAG CAGGCAGCTG AGCACTATAA GCTGCAGATG 
GAGAAAGCCA AAACACATTA TGATGCCAAG AAGCAGCAGA ACCAAGAGCT GCAGGAGCAG 
CTGCGGAGCC TGGAGCAGCT GCAGAAGGAA AACAAAGAGC TGCGAGCTGA AGCTGAACGG 
CTGGGCCATG AGCTACAGCA GGCTGGGCTG AAGACCAAGG AGGCTGAACA GACCTGCCGC 
CACCTTACTG CCCAGGTGCG CAGCCTGGAG GCACAGGTTG CCCATGCAGA CCAGCAGCTT 
CGAGACCTGG GCAAATTCCA GGTGGCAACT GATGCTTTAA AGAGCCGTGA GCCCCAGGCT 
AAGCCCCAGC TGGACTTGAG TATTGACAGC CTGGATCTGA GCTGCGAGGA GGGGACCCCA 
CTCAGTATCA CCAGACATAA AGCACTCATG ACAATTATAC CTGATTTGTC CCCCAACAAC 
CCTCTGAGCA AGCTGCCTCG TACCCAGCCA GACGGCACCA GCGTCCCTGG AGAACCAGCC 
TCACCTATCT CCCAGCGCCT GCCCCCCAAG GTAGAATCCC TGGAGAGTCT CTACTTCACT 
CCCATCCCTG CTCGGAGTCA GGCCCCCCTG GAGAGCAGCC TGGACTCCCT GGGAGACGTC 
TTCCTGGACT CGGGTCGTAA GACCCGCTCC GCTCGTCGGC GCACCACGCA GATCATCAAC 
ATCACCATGA CCAAGAAGCT AGATGTGGAA GAGCCAGACA GCGCCAACTC ATCGTTCTAC 
AGCACGCGGT CTGCTCCTGC TTCCCAGGCT AGCCTGCGAG CCACCTCCTC TACTCAGTCT 
CTAGCTCGCC TGGGTTCTCC CGATTATGGC AACTCAGCCC TGCTCAGCTT GCCTGGCTAC 
CGCCCCACCA CTCGCAGTTC TGCTCGTCGT TCCCAGGCCG GGGTGTCCAG TGGGGCCCCT 
CCAGGAAGGA ACAGCTTCTA CATGGGCACT TGCCAGGATG AGCCTGAGCA GCTGGATGAC 
TGGAACCGCA TTGCAGAGCT GCAGCAGCGC AATCGAGTGT GCCCCCCACA TCTGAAGACC 
TGCTATCCCC TGGAGTCCAG GCCTTCCCTG AGCCTGGGCA CCATCACAGA TGAGGAGATG 
AAAACTGGAG ACCCCCAAGA GACCCTGCGC CGAGCCAGCA TGCAGCCAAT CCAGATAGCC 
GAGGGCACTG GCATCACCAC CCGGCAGCAG CGCAAACGGG TCTCCCTAGA GCCCCACCAG 
GGCCCTGGAA CTCCTGAGTC TAAGAAGGCC ACCAGCTGTT TCCCACGCCC CATGACTCCC 
CGAGACCGAC ATGAAGGGCG CAAACAGAGC ACTACTGAGG CCCAGAAGAA AGCAGCTCCA 
GCTTCTACTA AACAGGCTGA CCGGCGCCAG TCGATGGCCT TCAGCATCCT CAACACACCC 
AAGAAGCTAG GGAACAGCCT TCTGCGGCGG GGAGCCTCAA AGAAGGCCCT GTCCAAGGCT 
TCCCCCAACA CTCGCAGTGG AACCCGCCGT TCTCCGCGCA TTGCCACCAC CACAGCCAGC 
GCCGCCACTG CTGCCGCCAT TGGTGCCACC CCTCGAGCCA AGGGCAAGGC AAAGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543367.1
CDS	161..6520
Translation	MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITRHKALMTIIPDLSPNNPLSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

Target ORF information:

RefSeq Version	XM_011545065.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545065.2

ORF Insert Sequence:

ATGACACTCC ACGCCACCCG GGGGGCTGCA CTCCTCTCTT GGGTGAACAG TCTACACGTG 
GCTGACCCTG TGGAGGCTGT GCTGCAGCTC CAGGACTGCA GCATCTTCAT CAAGATCATT 
GACAGAATCC ATGGCACTGA AGAGGGACAG CAAATCTTGA AGCAGCCGGT GTCAGAGAGA 
CTGGACTTTG TGTGCAGTTT TCTGCAGAAA AATCGAAAAC ATCCCTCTTC CCCAGAATGC 
CTGGTATCTG CACAGAAGGT GCTAGAGGGA TCAGAGCTGG AACTGGCGAA GATGACCATG 
CTGCTCTTAT ACCACTCTAC CATGAGCTCC AAAAGTCCCA GGGACTGGGA ACAGTTTGAA 
TATAAAATTC AGGCTGAGTT GGCTGTCATT CTTAAATTTG TGCTGGACCA TGAGGACGGG 
CTAAACCTTA ATGAGGACCT AGAGAACTTC CTACAGAAAG CTCCTGTGCC TTCTACCTGT 
TCTAGCACAT TCCCTGAAGA GCTCTCCCCA CCTAGCCACC AGGCCAAGAG GGAGATTCGC 
TTCCTAGAGC TACAGAAGGT TGCCTCCTCT TCCAGTGGGA ACAACTTTCT CTCAGGTTCT 
CCAGCTTCTC CCATGGGTGA TATCCTGCAG ACCCCACAGT TCCAGATGAG ACGGCTGAAG 
AAGCAGCTTG CTGATGAGAG AAGTAATAGG GATGAGCTGG AGCTGGAGCT AGCTGAGAAC 
CGCAAGCTCC TCACCGAGAA GGATGCACAG ATAGCCATGA TGCAGCAGCG CATTGACCGC 
CTAGCCCTGC TGAATGAGAA GCAGGCGGCC AGCCCACTGG AGCCCAAGGA GCTTGAGGAG 
CTGCGTGACA AGAATGAGAG CCTTACCATG CGGCTGCATG AAACCCTGAA GCAGTGCCAG 
GACCTGAAGA CAGAGAAGAG CCAGATGGAT CGCAAAATCA ACCAGCTTTC GGAGGAGAAT 
GGAGACCTTT CCTTTAAGCT GCGGGAGTTT GCCAGTCATC TGCAGCAGCT ACAGGATGCC 
CTCAATGAGC TGACGGAGGA GCACAGCAAG GCCACTCAGG AGTGGCTAGA GAAGCAGGCC 
CAGCTGGAGA AGGAGCTCAG CGCAGCCCTG CAGGACAAGA AATGCCTTGA AGAGAAGAAC 
GAAATCCTTC AGGGAAAACT TTCACAGCTG GAAGAACACT TGTCCCAGCT GCAGGATAAC 
CCACCCCAGG AGAAGGGCGA GGTGCTGGGT GATGTCTTGC AGCTGGAAAC CTTGAAGCAA 
GAGGCAGCCA CTCTTGCTGC AAACAACACA CAGCTCCAAG CCAGGGTAGA GATGCTGGAG 
ACTGAGCGAG GCCAGCAGGA AGCCAAGCTG CTTGCTGAGC GGGGCCACTT CGAAGAAGAA 
AAGCAGCAGC TGTCTAGCCT GATCACTGAC CTGCAGAGCT CCATCTCCAA CCTCAGCCAG 
GCCAAGGAAG AGCTGGAGCA GGCCTCCCAG GCTCATGGGG CCCGGTTGAC TGCCCAGGTG 
GCCTCTCTGA CCTCTGAGCT CACCACACTC AATGCCACCA TCCAGCAACA GGATCAAGAA 
CTGGCTGGCC TGAAGCAGCA GGCCAAAGAG AAGCAGGCCC AGCTAGCACA GACCCTCCAA 
CAGCAAGAAC AGGCCTCCCA GGGCCTCCGC CACCAGGTGG AGCAGCTAAG CAGTAGCCTG 
AAGCAGAAGG AGCAGCAGTT GAAGGAGGTA GCGGAGAAGC AGGAGGCAAC TAGGCAGGAC 
CATGCCCAGC AACTGGCCAC TGCTGCAGAG GAGCGAGAGG CCTCCTTAAG GGAGCGGGAT 
GCGGCTCTCA AGCAGCTGGA GGCACTGGAG AAGGAGAAGG CTGCCAAGCT GGAGATTCTG 
CAGCAGCAAC TTCAGGTGGC TAATGAAGCC CGGGACAGTG CCCAGACCTC AGTGACACAG 
GCCCAGCGGG AGAAGGCAGA GCTGAGCCGG AAGGTGGAGG AACTCCAGGC CTGTGTTGAG 
ACAGCCCGCC AGGAACAGCA TGAGGCCCAG GCCCAGGTTG CAGAGCTAGA GTTGCAGCTG 
CGGTCTGAGC AGCAAAAAGC AACTGAGAAA GAAAGGGTGG CCCAGGAGAA GGACCAGCTC 
CAGGAGCAGC TCCAGGCCCT CAAAGAGTCC TTGAAGGTCA CCAAGGGCAG CCTTGAAGAG 
GAGAAGCGCA GGGCTGCAGA TGCCCTGGAA GAGCAGCAGC GTTGTATCTC TGAGCTGAAG 
GCAGAGACCC GAAGCCTGGT GGAGCAGCAT AAGCGGGAAC GAAAGGAGCT GGAAGAAGAG 
AGGGCTGGGC GCAAGGGGCT GGAGGCTCGA TTACAGCAGC TTGGGGAGGC CCATCAGGCT 
GAGACTGAAG TCCTGCGGCG GGAGCTGGCA GAGGCCATGG CTGCCCAGCA CACAGCTGAG 
AGTGAGTGTG AGCAGCTCGT CAAAGAAGTA GCTGCCTGGC GTGAGCGGTA TGAGGATAGC 
CAGCAAGAGG AGGCACAGTA TGGCGCCATG TTCCAGGAAC AGCTGATGAC TTTGAAGGAG 
GAATGTGAGA AGGCCCGCCA GGAGCTGCAG GAGGCAAAGG AGAAGGTGGC AGGCATAGAA 
TCCCACAGCG AGCTCCAGAT AAGCCGGCAG CAGAACGAAC TAGCTGAGCT CCATGCCAAC 
CTGGCCAGAG CACTCCAGCA GGTCCAAGAG AAGGAAGTCA GGGCCCAGAA GCTTGCAGAT 
GACCTCTCCA CTCTGCAGGA AAAGATGGCT GCCACCAGCA AAGAGGTGGC CCGCTTGGAG 
ACCTTGGTGC GCAAGGCAGG TGAGCAGCAG GAAACAGCCT CCCGGGAGTT AGTCAAGGAG 
CCTGCGAGGG CAGGAGACAG ACAGCCCGAG TGGCTGGAAG AGCAACAGGG ACGCCAGTTC 
TGCAGCACAC AGGCAGCGCT GCAGGCTATG GAGCGGGAGG CAGAGCAGAT GGGCAATGAG 
CTGGAACGGC TGCGGGCCGC GCTGATGGAG AGCCAGGGGC AGCAGCAGGA GGAGCGTGGG 
CAGCAGGAAA GGGAGGTGGC GCGGCTGACC CAGGAGCGGG GCCGTGCCCA GGCTGACCTT 
GCCCTGGAGA AGGCGGCCAG AGCAGAGCTT GAGATGCGGC TGCAGAACGC CCTCAACGAG 
CAGCGTGTGG AGTTCGCTAC CCTGCAAGAG GCACTGGCTC ATGCCCTGAC GGAAAAGGAA 
GGCAAGGACC AGGAGTTGGC CAAGCTTCGT GGTCTGGAGG CAGCCCAGAT AAAAGAGCTG 
GAGGAACTTC GGCAAACCGT GAAGCAACTG AAGGAACAGC TGGCTAAGAA AGAAAAGGAG 
CACGCATCTG GCTCAGGAGC CCAATCTGAG GCTGCTGGCA GGACAGAGCC AACAGGCCCC 
AAGCTGGAGG CACTGCGGGC AGAGGTGAGC AAGCTGGAAC AGCAATGCCA GAAGCAGCAG 
GAGCAGGCTG ACAGCCTGGA ACGCAGCCTC GAGGCTGAGC GGGCCTCCCG GGCTGAGCGG 
GACAGTGCTC TGGAGACTCT GCAGGGCCAG TTAGAGGAGA AGGCCCAGGA GCTAGGGCAC 
AGTCAGAGTG CCTTAGCCTC GGCCCAACGG GAGTTGGCTG CCTTCCGCAC CAAGGTACAA 
GACCACAGCA AGGCTGAAGA TGAGTGGAAG GCCCAGGTGG CCCGGGGCCG GCAAGAGGCT 
GAGAGGAAAA ATAGCCTCAT CAGCAGCTTG GAGGAGGAGG TGTCCATCCT GAATCGCCAG 
GTCCTGGAGA AGGAGGGGGA GAGCAAGGAG TTGAAGCGGC TGGTGATGGC CGAGTCAGAG 
AAGAGCCAGA AGCTGGAGGA GAGGCTGCGC CTGCTGCAGG CAGAGACAGC CAGCAACAGT 
GCCAGAGCTG CAGAACGCAG CTCTGCTCTG CGGGAGGAGG TGCAGAGCCT CCGGGAGGAG 
GCTGAGAAAC AGCGGGTGGC TTCAGAGAAC CTGCGGCAGG AGCTGACCTC ACAGGCTGAG 
CGTGCGGAGG AGCTGGGCCA AGAATTGAAG GCGTGGCAGG AGAAGTTCTT CCAGAAAGAG 
CAGGCCCTCT CCACCCTGCA GCTCGAGCAC ACCAGCACAC AGGCCCTGGT GAGTGAGCTG 
CTGCCAGCTA AGCACCTCTG CCAGCAGCTG CAGGCCGAGC AGGCCGCTGC CGAGAAACGC 
CACCGTGAGG AGCTGGAGCA GAGCAAGCAG GCCGCTGGGG GACTGCGGGC AGAGCTGCTG 
CGGGCCCAGC GGGAGCTTGG GGAGCTGATT CCTCTGCGGC AGAAGGTGGC AGAGCAGGAG 
CGAACAGCTC AGCAGCTGCG GGCAGAGAAG GCCAGCTATG CAGAGCAGCT GAGCATGCTG 
AAGAAGGCGC ATGGCCTGCT GGCAGAGGAG AACCGGGGGC TGGGTGAGCG GGCCAACCTT 
GGCCGGCAGT TTCTGGAAGT GGAGTTGGAC CAGGCCCGGG AGAAGTATGT CCAAGAGTTG 
GCAGCCGTAC GTGCTGATGC TGAGACCCGT CTGGCTGAGG TGCAGCGAGA AGCACAGAGC 
ACTGCCCGGG AGCTGGAGGT GATGACTGCC AAGTATGAGG GTGCCAAGGT CAAGGTCCTG 
GAGGAGAGGC AGCGGTTCCA GGAAGAGAGG CAGAAACTCA CTGCCCAGGT GGAAGAACTG 
AGTAAGAAAC TGGCTGACTC TGACCAAGCC AGCAAGGTGC AGCAGCAGAA GCTGAAGGCT 
GTCCAGGCTC AGGGAGGCGA GAGCCAGCAG GAGGCCCAGC GCCTCCAGGC CCAGCTGAAT 
GAACTGCAAG CCCAGTTGAG CCAGAAGGAG CAGGCAGCTG AGCACTATAA GCTGCAGATG 
GAGAAAGCCA AAACACATTA TGATGCCAAG AAGCAGCAGA ACCAAGAGCT GCAGGAGCAG 
CTGCGGAGCC TGGAGCAGCT GCAGAAGGAA AACAAAGAGC TGCGAGCTGA AGCTGAACGG 
CTGGGCCATG AGCTACAGCA GGCTGGGCTG AAGACCAAGG AGGCTGAACA GACCTGCCGC 
CACCTTACTG CCCAGGTGCG CAGCCTGGAG GCACAGGTTG CCCATGCAGA CCAGCAGCTT 
CGAGACCTGG GCAAATTCCA GGTGGCAACT GATGCTTTAA AGAGCCGTGA GCCCCAGGCT 
AAGCCCCAGC TGGACTTGAG TATTGACAGC CTGGATCTGA GCTGCGAGGA GGGGACCCCA 
CTCAGTATCA CCAGACATAA AGCACTCATG ACAATTATAC CTGATTTGTC CCCCAACAAC 
CCTCTGAGCA AGCTGCCTCG TACCCAGCCA GACGGCACCA GCGTCCCTGG AGAACCAGCC 
TCACCTATCT CCCAGCGCCT GCCCCCCAAG GTAGAATCCC TGGAGAGTCT CTACTTCACT 
CCCATCCCTG CTCGGAGTCA GGCCCCCCTG GAGAGCAGCC TGGACTCCCT GGGAGACGTC 
TTCCTGGACT CGGGTCGTAA GACCCGCTCC GCTCGTCGGC GCACCACGCA GATCATCAAC 
ATCACCATGA CCAAGAAGCT AGATGTGGAA GAGCCAGACA GCGCCAACTC ATCGTTCTAC 
AGCACGCGGT CTGCTCCTGC TTCCCAGGCT AGCCTGCGAG CCACCTCCTC TACTCAGTCT 
CTAGCTCGCC TGGGTTCTCC CGATTATGGC AACTCAGCCC TGCTCAGCTT GCCTGGCTAC 
CGCCCCACCA CTCGCAGTTC TGCTCGTCGT TCCCAGGCCG GGGTGTCCAG TGGGGCCCCT 
CCAGGAAGGA ACAGCTTCTA CATGGGCACT TGCCAGGATG AGCCTGAGCA GCTGGATGAC 
TGGAACCGCA TTGCAGAGCT GCAGCAGCGC AATCGAGTGT GCCCCCCACA TCTGAAGACC 
TGCTATCCCC TGGAGTCCAG GCCTTCCCTG AGCCTGGGCA CCATCACAGA TGAGGAGATG 
AAAACTGGAG ACCCCCAAGA GACCCTGCGC CGAGCCAGCA TGCAGCCAAT CCAGATAGCC 
GAGGGCACTG GCATCACCAC CCGGCAGCAG CGCAAACGGG TCTCCCTAGA GCCCCACCAG 
GGCCCTGGAA CTCCTGAGTC TAAGAAGGCC ACCAGCTGTT TCCCACGCCC CATGACTCCC 
CGAGACCGAC ATGAAGGGCG CAAACAGAGC ACTACTGAGG CCCAGAAGAA AGCAGCTCCA 
GCTTCTACTA AACAGGCTGA CCGGCGCCAG TCGATGGCCT TCAGCATCCT CAACACACCC 
AAGAAGCTAG GGAACAGCCT TCTGCGGCGG GGAGCCTCAA AGAAGGCCCT GTCCAAGGCT 
TCCCCCAACA CTCGCAGTGG AACCCGCCGT TCTCCGCGCA TTGCCACCAC CACAGCCAGC 
GCCGCCACTG CTGCCGCCAT TGGTGCCACC CCTCGAGCCA AGGGCAAGGC AAAGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NUMA1
Entrez Gene ID	4926
Full Name	nuclear mitotic apparatus protein 1
Synonyms	NMP-22,NUMA
General protein information	Preferred Names nuclear mitotic apparatus protein 1 Names nuclear mitotic apparatus protein 1 SP-H antigen centrophilin stabilizes mitotic spindle in mitotic cells nuclear matrix protein-22 structural nuclear protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.4
Summary	This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013].
Disorder MIM:	164009
Disorder Html:	Leukemia, acute promyelocytic, somatic, 612376 (3)

Gallus gallus (chicken)	NUMA1	NP_001177854.1
Pan troglodytes (chimpanzee)	NUMA1	XP_001174474.1
	NUMA1	XP_001114111.2
Rattus norvegicus (Norway rat)	Numa1	NP_001094161.1
Homo sapiens (human)	NUMA1	NP_006176.2
Canis lupus familiaris (dog)	NUMA1	XP_005633612.1
Bos taurus (cattle)	NUMA1	NP_001192675.1
Xenopus tropicalis (tropical clawed frog)	LOC100145664	XP_004920191.1
Mus musculus (house mouse)	Numa1	NP_598708.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NUMA1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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