numa1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following numa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the numa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa53058 XM_031897416.1
Latest version!
Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa53058 XM_031897415.1
Latest version!
Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa53058 XM_031897417.1
Latest version!
Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa53059 XM_031897418.1
Latest version!
Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa53060 XM_031897419.1
Latest version!
Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa53061 XM_031897420.1
Latest version!
Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa53062 XM_031897421.1
Latest version!
Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa19633 XM_004920135.3
Latest version!
Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OXa19634 XM_004920136.3
Latest version!
Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OXa31960 XM_012954898.2
Latest version!
Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OXa31961 XM_012954899.2
Latest version!
Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OXa31962 XM_012954900.2
Latest version!
Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa53058
    Clone ID Related Accession (Same CDS sequence) XM_031897415.1 , XM_031897416.1 , XM_031897417.1
    Accession Version XM_031897415.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nuclear mitotic apparatus protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CAGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTGT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGTGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAAGAAAC AACTGGAACG CAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGGAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGGAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAA TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGAGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCG
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAA
    GAGCTGGAGG CGGCAAAGAA AGAGCAAAGT GAGCAGGTGC AGGAGCTGAA CAAGCAGCTG
    AGCCAGCAGG AGAAGGCCTT ACGGAGCCAG CAGCACAAGC TGAAGCAGCA GGAAGGTGAG
    ACTCATGAGG AGGCTGAGAA GGCTCAGAAG CGAGTGGTGG AGTTGGAGTC CCAGGTGGAA
    CAGCAGACTC AGGCCGTGGA ACATTACAAA GCTCAGATGG AGAAAGCCAA GCTGTATTAC
    GATGCCAAGA AGAAGCAGAA CCAGGAGCTA AGCGAGGAGC TGCAAAGCCA CATTAGAGAG
    CAGGAACATC TGCGCAAGGA GAACGTAGAT CTAAAGGCAG AGTCTGAGCA GCTCAGTAAG
    GAGCTACAGC ATTCGCTGCT ACAGAACAAA GAGGCAGAGC AAAACTGCAA GAACCTGTCC
    AATCGGGTTA GGAGTCTGGA GGCCCAGGTG GAGTATGCCG ACCGGCAGCT GCGAGACCTG
    GGGAAGTTCC AGTTGGCCAC GGATTCCATG AAGAGTCGGG AAACTTTCGG AGCCCCCCGT
    GTAACACGGA GCCATGCAGA TGTCAGTATA GACAGCCTGG ACTTGAGCTT TGAGGATGAC
    CAGCTTTTGA ACTCAACAAG TAAGAATGGC CGCTGCAACG AGGAACCTGC CACATCGTCA
    GTACATGCCA GCAGCATGGA GTCTCCTACC ACAGGCCAAC TGCCCAAAAA GGTGGAGTCT
    CTGGAAAGCC TCTACTTCAC ACCTATTCCA ACCCGGGCAC AGTCCAAACT GGAAAGTAGC
    CTTGGTTCCA TTGGGGACCT GTCCCTAGAC TCCAGCAAGA AAACGCGGTC TGCAAGGAGA
    CGCACCACCC AGATCATTAA CATCACAATG ACCAAGAAGA CAAAGGAGGA ACCGGAAGCA
    GAGAGTGCCA ACACGTCATT CTACAGCCTG CGCTCCGCTC CGTCCTATCA GAGTCTCCAC
    CAGCAGAAGC CCCGGAGGCC TGGGCGTCCT CAGGCAGCGG TTTCTGCCCC AGCCCTGGCC
    AGCCTCCCAT CCCAGGAATC CCTGGCCAAG ACAGAACAGT TCTCTGATGA CAGCTTTAAT
    AACTCCGTAT TGATGAATTT ACCTGGTTAC CGACCCCCCA CGCGTAGATC TGCAAGGCTT
    TCACAAACTG GAGGCCGAAG CAGTTTCTAC ATGAGCACGT GCCAGGATGA GCCAGACCCA
    CAGGAAGACT GGAACCGGAT CGCTGAGTTG CAGGCCCGGA ACAAGGCCTG CCCACCCCAC
    CTCAAGACTT CCTACCCACT GGAGTCCAGG CCGTCACTCT TAAGCTCCAC CATTACCGAT
    GAAGAGGTCA AGTTGGGAGA CCCTAAAGAG ACGCTCAGGA GAGCCACCAT GCTGCCCTCC
    CAGATACAAG AGTCCACAGG CAGTACCCGC AGAATGAGCT TGGCTGCCTC TGAACATATG
    AGGGGACCCA GCATCAGCAC CAGGCAGCAG ATGAAGCGCG TGTCCGAGGA GTCTCACCTT
    GGCCCAGACA CCCCAGAAGC CAAGAAGTCT GCCACTTGCT TTCCTCGGCC CATGACCCCA
    AAGGATAAAC ACGACGCACG CAGATTGTCC ACTGTGGAAA GCAAAGGAAA CTCCAGCCAT
    CAGCAGGCGC AGCCCACTCG CCGTCAGTCT ACAGCATTTA GCATTTTCAA CACCCCCAGA
    AAGCTGGGCA ACAGCCTCCT GAAGAGAGGC CTCAACAAGA AAACGACTCC CAAAAATAGC
    CCCCGGGGGC GGGGGGCAAG TGGCAGCGCC GGCAGCACTT CAGGCAAATC CCCGCACCGC
    TCTCTCCGGA AGTCACCCAG CAGGCGGTCT CCGAGGGCCT CCACTGCAAA GTCCCCCAAA
    GCTTCAACCA AGGTGGGGCA GGAGCTGCAG ATACAGTTTT TCGAGCGCAA GCATCAGAGG
    AACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753275.1
    CDS132..7040
    Translation

    Target ORF information:

    RefSeq Version XM_031897415.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897415.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CAGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTGT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGTGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAAGAAAC AACTGGAACG CAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGGAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGGAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAA TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGAGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCG
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAA
    GAGCTGGAGG CGGCAAAGAA AGAGCAAAGT GAGCAGGTGC AGGAGCTGAA CAAGCAGCTG
    AGCCAGCAGG AGAAGGCCTT ACGGAGCCAG CAGCACAAGC TGAAGCAGCA GGAAGGTGAG
    ACTCATGAGG AGGCTGAGAA GGCTCAGAAG CGAGTGGTGG AGTTGGAGTC CCAGGTGGAA
    CAGCAGACTC AGGCCGTGGA ACATTACAAA GCTCAGATGG AGAAAGCCAA GCTGTATTAC
    GATGCCAAGA AGAAGCAGAA CCAGGAGCTA AGCGAGGAGC TGCAAAGCCA CATTAGAGAG
    CAGGAACATC TGCGCAAGGA GAACGTAGAT CTAAAGGCAG AGTCTGAGCA GCTCAGTAAG
    GAGCTACAGC ATTCGCTGCT ACAGAACAAA GAGGCAGAGC AAAACTGCAA GAACCTGTCC
    AATCGGGTTA GGAGTCTGGA GGCCCAGGTG GAGTATGCCG ACCGGCAGCT GCGAGACCTG
    GGGAAGTTCC AGTTGGCCAC GGATTCCATG AAGAGTCGGG AAACTTTCGG AGCCCCCCGT
    GTAACACGGA GCCATGCAGA TGTCAGTATA GACAGCCTGG ACTTGAGCTT TGAGGATGAC
    CAGCTTTTGA ACTCAACAAG TAAGAATGGC CGCTGCAACG AGGAACCTGC CACATCGTCA
    GTACATGCCA GCAGCATGGA GTCTCCTACC ACAGGCCAAC TGCCCAAAAA GGTGGAGTCT
    CTGGAAAGCC TCTACTTCAC ACCTATTCCA ACCCGGGCAC AGTCCAAACT GGAAAGTAGC
    CTTGGTTCCA TTGGGGACCT GTCCCTAGAC TCCAGCAAGA AAACGCGGTC TGCAAGGAGA
    CGCACCACCC AGATCATTAA CATCACAATG ACCAAGAAGA CAAAGGAGGA ACCGGAAGCA
    GAGAGTGCCA ACACGTCATT CTACAGCCTG CGCTCCGCTC CGTCCTATCA GAGTCTCCAC
    CAGCAGAAGC CCCGGAGGCC TGGGCGTCCT CAGGCAGCGG TTTCTGCCCC AGCCCTGGCC
    AGCCTCCCAT CCCAGGAATC CCTGGCCAAG ACAGAACAGT TCTCTGATGA CAGCTTTAAT
    AACTCCGTAT TGATGAATTT ACCTGGTTAC CGACCCCCCA CGCGTAGATC TGCAAGGCTT
    TCACAAACTG GAGGCCGAAG CAGTTTCTAC ATGAGCACGT GCCAGGATGA GCCAGACCCA
    CAGGAAGACT GGAACCGGAT CGCTGAGTTG CAGGCCCGGA ACAAGGCCTG CCCACCCCAC
    CTCAAGACTT CCTACCCACT GGAGTCCAGG CCGTCACTCT TAAGCTCCAC CATTACCGAT
    GAAGAGGTCA AGTTGGGAGA CCCTAAAGAG ACGCTCAGGA GAGCCACCAT GCTGCCCTCC
    CAGATACAAG AGTCCACAGG CAGTACCCGC AGAATGAGCT TGGCTGCCTC TGAACATATG
    AGGGGACCCA GCATCAGCAC CAGGCAGCAG ATGAAGCGCG TGTCCGAGGA GTCTCACCTT
    GGCCCAGACA CCCCAGAAGC CAAGAAGTCT GCCACTTGCT TTCCTCGGCC CATGACCCCA
    AAGGATAAAC ACGACGCACG CAGATTGTCC ACTGTGGAAA GCAAAGGAAA CTCCAGCCAT
    CAGCAGGCGC AGCCCACTCG CCGTCAGTCT ACAGCATTTA GCATTTTCAA CACCCCCAGA
    AAGCTGGGCA ACAGCCTCCT GAAGAGAGGC CTCAACAAGA AAACGACTCC CAAAAATAGC
    CCCCGGGGGC GGGGGGCAAG TGGCAGCGCC GGCAGCACTT CAGGCAAATC CCCGCACCGC
    TCTCTCCGGA AGTCACCCAG CAGGCGGTCT CCGAGGGCCT CCACTGCAAA GTCCCCCAAA
    GCTTCAACCA AGGTGGGGCA GGAGCTGCAG ATACAGTTTT TCGAGCGCAA GCATCAGAGG
    AACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53058
    Clone ID Related Accession (Same CDS sequence) XM_031897415.1 , XM_031897416.1 , XM_031897417.1
    Accession Version XM_031897416.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nuclear mitotic apparatus protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CAGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTGT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGTGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAAGAAAC AACTGGAACG CAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGGAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGGAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAA TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGAGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCG
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAA
    GAGCTGGAGG CGGCAAAGAA AGAGCAAAGT GAGCAGGTGC AGGAGCTGAA CAAGCAGCTG
    AGCCAGCAGG AGAAGGCCTT ACGGAGCCAG CAGCACAAGC TGAAGCAGCA GGAAGGTGAG
    ACTCATGAGG AGGCTGAGAA GGCTCAGAAG CGAGTGGTGG AGTTGGAGTC CCAGGTGGAA
    CAGCAGACTC AGGCCGTGGA ACATTACAAA GCTCAGATGG AGAAAGCCAA GCTGTATTAC
    GATGCCAAGA AGAAGCAGAA CCAGGAGCTA AGCGAGGAGC TGCAAAGCCA CATTAGAGAG
    CAGGAACATC TGCGCAAGGA GAACGTAGAT CTAAAGGCAG AGTCTGAGCA GCTCAGTAAG
    GAGCTACAGC ATTCGCTGCT ACAGAACAAA GAGGCAGAGC AAAACTGCAA GAACCTGTCC
    AATCGGGTTA GGAGTCTGGA GGCCCAGGTG GAGTATGCCG ACCGGCAGCT GCGAGACCTG
    GGGAAGTTCC AGTTGGCCAC GGATTCCATG AAGAGTCGGG AAACTTTCGG AGCCCCCCGT
    GTAACACGGA GCCATGCAGA TGTCAGTATA GACAGCCTGG ACTTGAGCTT TGAGGATGAC
    CAGCTTTTGA ACTCAACAAG TAAGAATGGC CGCTGCAACG AGGAACCTGC CACATCGTCA
    GTACATGCCA GCAGCATGGA GTCTCCTACC ACAGGCCAAC TGCCCAAAAA GGTGGAGTCT
    CTGGAAAGCC TCTACTTCAC ACCTATTCCA ACCCGGGCAC AGTCCAAACT GGAAAGTAGC
    CTTGGTTCCA TTGGGGACCT GTCCCTAGAC TCCAGCAAGA AAACGCGGTC TGCAAGGAGA
    CGCACCACCC AGATCATTAA CATCACAATG ACCAAGAAGA CAAAGGAGGA ACCGGAAGCA
    GAGAGTGCCA ACACGTCATT CTACAGCCTG CGCTCCGCTC CGTCCTATCA GAGTCTCCAC
    CAGCAGAAGC CCCGGAGGCC TGGGCGTCCT CAGGCAGCGG TTTCTGCCCC AGCCCTGGCC
    AGCCTCCCAT CCCAGGAATC CCTGGCCAAG ACAGAACAGT TCTCTGATGA CAGCTTTAAT
    AACTCCGTAT TGATGAATTT ACCTGGTTAC CGACCCCCCA CGCGTAGATC TGCAAGGCTT
    TCACAAACTG GAGGCCGAAG CAGTTTCTAC ATGAGCACGT GCCAGGATGA GCCAGACCCA
    CAGGAAGACT GGAACCGGAT CGCTGAGTTG CAGGCCCGGA ACAAGGCCTG CCCACCCCAC
    CTCAAGACTT CCTACCCACT GGAGTCCAGG CCGTCACTCT TAAGCTCCAC CATTACCGAT
    GAAGAGGTCA AGTTGGGAGA CCCTAAAGAG ACGCTCAGGA GAGCCACCAT GCTGCCCTCC
    CAGATACAAG AGTCCACAGG CAGTACCCGC AGAATGAGCT TGGCTGCCTC TGAACATATG
    AGGGGACCCA GCATCAGCAC CAGGCAGCAG ATGAAGCGCG TGTCCGAGGA GTCTCACCTT
    GGCCCAGACA CCCCAGAAGC CAAGAAGTCT GCCACTTGCT TTCCTCGGCC CATGACCCCA
    AAGGATAAAC ACGACGCACG CAGATTGTCC ACTGTGGAAA GCAAAGGAAA CTCCAGCCAT
    CAGCAGGCGC AGCCCACTCG CCGTCAGTCT ACAGCATTTA GCATTTTCAA CACCCCCAGA
    AAGCTGGGCA ACAGCCTCCT GAAGAGAGGC CTCAACAAGA AAACGACTCC CAAAAATAGC
    CCCCGGGGGC GGGGGGCAAG TGGCAGCGCC GGCAGCACTT CAGGCAAATC CCCGCACCGC
    TCTCTCCGGA AGTCACCCAG CAGGCGGTCT CCGAGGGCCT CCACTGCAAA GTCCCCCAAA
    GCTTCAACCA AGGTGGGGCA GGAGCTGCAG ATACAGTTTT TCGAGCGCAA GCATCAGAGG
    AACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753276.1
    CDS238..7146
    Translation

    Target ORF information:

    RefSeq Version XM_031897416.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897416.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CAGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTGT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGTGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAAGAAAC AACTGGAACG CAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGGAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGGAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAA TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGAGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCG
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAA
    GAGCTGGAGG CGGCAAAGAA AGAGCAAAGT GAGCAGGTGC AGGAGCTGAA CAAGCAGCTG
    AGCCAGCAGG AGAAGGCCTT ACGGAGCCAG CAGCACAAGC TGAAGCAGCA GGAAGGTGAG
    ACTCATGAGG AGGCTGAGAA GGCTCAGAAG CGAGTGGTGG AGTTGGAGTC CCAGGTGGAA
    CAGCAGACTC AGGCCGTGGA ACATTACAAA GCTCAGATGG AGAAAGCCAA GCTGTATTAC
    GATGCCAAGA AGAAGCAGAA CCAGGAGCTA AGCGAGGAGC TGCAAAGCCA CATTAGAGAG
    CAGGAACATC TGCGCAAGGA GAACGTAGAT CTAAAGGCAG AGTCTGAGCA GCTCAGTAAG
    GAGCTACAGC ATTCGCTGCT ACAGAACAAA GAGGCAGAGC AAAACTGCAA GAACCTGTCC
    AATCGGGTTA GGAGTCTGGA GGCCCAGGTG GAGTATGCCG ACCGGCAGCT GCGAGACCTG
    GGGAAGTTCC AGTTGGCCAC GGATTCCATG AAGAGTCGGG AAACTTTCGG AGCCCCCCGT
    GTAACACGGA GCCATGCAGA TGTCAGTATA GACAGCCTGG ACTTGAGCTT TGAGGATGAC
    CAGCTTTTGA ACTCAACAAG TAAGAATGGC CGCTGCAACG AGGAACCTGC CACATCGTCA
    GTACATGCCA GCAGCATGGA GTCTCCTACC ACAGGCCAAC TGCCCAAAAA GGTGGAGTCT
    CTGGAAAGCC TCTACTTCAC ACCTATTCCA ACCCGGGCAC AGTCCAAACT GGAAAGTAGC
    CTTGGTTCCA TTGGGGACCT GTCCCTAGAC TCCAGCAAGA AAACGCGGTC TGCAAGGAGA
    CGCACCACCC AGATCATTAA CATCACAATG ACCAAGAAGA CAAAGGAGGA ACCGGAAGCA
    GAGAGTGCCA ACACGTCATT CTACAGCCTG CGCTCCGCTC CGTCCTATCA GAGTCTCCAC
    CAGCAGAAGC CCCGGAGGCC TGGGCGTCCT CAGGCAGCGG TTTCTGCCCC AGCCCTGGCC
    AGCCTCCCAT CCCAGGAATC CCTGGCCAAG ACAGAACAGT TCTCTGATGA CAGCTTTAAT
    AACTCCGTAT TGATGAATTT ACCTGGTTAC CGACCCCCCA CGCGTAGATC TGCAAGGCTT
    TCACAAACTG GAGGCCGAAG CAGTTTCTAC ATGAGCACGT GCCAGGATGA GCCAGACCCA
    CAGGAAGACT GGAACCGGAT CGCTGAGTTG CAGGCCCGGA ACAAGGCCTG CCCACCCCAC
    CTCAAGACTT CCTACCCACT GGAGTCCAGG CCGTCACTCT TAAGCTCCAC CATTACCGAT
    GAAGAGGTCA AGTTGGGAGA CCCTAAAGAG ACGCTCAGGA GAGCCACCAT GCTGCCCTCC
    CAGATACAAG AGTCCACAGG CAGTACCCGC AGAATGAGCT TGGCTGCCTC TGAACATATG
    AGGGGACCCA GCATCAGCAC CAGGCAGCAG ATGAAGCGCG TGTCCGAGGA GTCTCACCTT
    GGCCCAGACA CCCCAGAAGC CAAGAAGTCT GCCACTTGCT TTCCTCGGCC CATGACCCCA
    AAGGATAAAC ACGACGCACG CAGATTGTCC ACTGTGGAAA GCAAAGGAAA CTCCAGCCAT
    CAGCAGGCGC AGCCCACTCG CCGTCAGTCT ACAGCATTTA GCATTTTCAA CACCCCCAGA
    AAGCTGGGCA ACAGCCTCCT GAAGAGAGGC CTCAACAAGA AAACGACTCC CAAAAATAGC
    CCCCGGGGGC GGGGGGCAAG TGGCAGCGCC GGCAGCACTT CAGGCAAATC CCCGCACCGC
    TCTCTCCGGA AGTCACCCAG CAGGCGGTCT CCGAGGGCCT CCACTGCAAA GTCCCCCAAA
    GCTTCAACCA AGGTGGGGCA GGAGCTGCAG ATACAGTTTT TCGAGCGCAA GCATCAGAGG
    AACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53058
    Clone ID Related Accession (Same CDS sequence) XM_031897415.1 , XM_031897416.1 , XM_031897417.1
    Accession Version XM_031897417.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nuclear mitotic apparatus protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CAGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTGT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGTGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAAGAAAC AACTGGAACG CAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGGAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGGAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAA TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGAGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCG
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAA
    GAGCTGGAGG CGGCAAAGAA AGAGCAAAGT GAGCAGGTGC AGGAGCTGAA CAAGCAGCTG
    AGCCAGCAGG AGAAGGCCTT ACGGAGCCAG CAGCACAAGC TGAAGCAGCA GGAAGGTGAG
    ACTCATGAGG AGGCTGAGAA GGCTCAGAAG CGAGTGGTGG AGTTGGAGTC CCAGGTGGAA
    CAGCAGACTC AGGCCGTGGA ACATTACAAA GCTCAGATGG AGAAAGCCAA GCTGTATTAC
    GATGCCAAGA AGAAGCAGAA CCAGGAGCTA AGCGAGGAGC TGCAAAGCCA CATTAGAGAG
    CAGGAACATC TGCGCAAGGA GAACGTAGAT CTAAAGGCAG AGTCTGAGCA GCTCAGTAAG
    GAGCTACAGC ATTCGCTGCT ACAGAACAAA GAGGCAGAGC AAAACTGCAA GAACCTGTCC
    AATCGGGTTA GGAGTCTGGA GGCCCAGGTG GAGTATGCCG ACCGGCAGCT GCGAGACCTG
    GGGAAGTTCC AGTTGGCCAC GGATTCCATG AAGAGTCGGG AAACTTTCGG AGCCCCCCGT
    GTAACACGGA GCCATGCAGA TGTCAGTATA GACAGCCTGG ACTTGAGCTT TGAGGATGAC
    CAGCTTTTGA ACTCAACAAG TAAGAATGGC CGCTGCAACG AGGAACCTGC CACATCGTCA
    GTACATGCCA GCAGCATGGA GTCTCCTACC ACAGGCCAAC TGCCCAAAAA GGTGGAGTCT
    CTGGAAAGCC TCTACTTCAC ACCTATTCCA ACCCGGGCAC AGTCCAAACT GGAAAGTAGC
    CTTGGTTCCA TTGGGGACCT GTCCCTAGAC TCCAGCAAGA AAACGCGGTC TGCAAGGAGA
    CGCACCACCC AGATCATTAA CATCACAATG ACCAAGAAGA CAAAGGAGGA ACCGGAAGCA
    GAGAGTGCCA ACACGTCATT CTACAGCCTG CGCTCCGCTC CGTCCTATCA GAGTCTCCAC
    CAGCAGAAGC CCCGGAGGCC TGGGCGTCCT CAGGCAGCGG TTTCTGCCCC AGCCCTGGCC
    AGCCTCCCAT CCCAGGAATC CCTGGCCAAG ACAGAACAGT TCTCTGATGA CAGCTTTAAT
    AACTCCGTAT TGATGAATTT ACCTGGTTAC CGACCCCCCA CGCGTAGATC TGCAAGGCTT
    TCACAAACTG GAGGCCGAAG CAGTTTCTAC ATGAGCACGT GCCAGGATGA GCCAGACCCA
    CAGGAAGACT GGAACCGGAT CGCTGAGTTG CAGGCCCGGA ACAAGGCCTG CCCACCCCAC
    CTCAAGACTT CCTACCCACT GGAGTCCAGG CCGTCACTCT TAAGCTCCAC CATTACCGAT
    GAAGAGGTCA AGTTGGGAGA CCCTAAAGAG ACGCTCAGGA GAGCCACCAT GCTGCCCTCC
    CAGATACAAG AGTCCACAGG CAGTACCCGC AGAATGAGCT TGGCTGCCTC TGAACATATG
    AGGGGACCCA GCATCAGCAC CAGGCAGCAG ATGAAGCGCG TGTCCGAGGA GTCTCACCTT
    GGCCCAGACA CCCCAGAAGC CAAGAAGTCT GCCACTTGCT TTCCTCGGCC CATGACCCCA
    AAGGATAAAC ACGACGCACG CAGATTGTCC ACTGTGGAAA GCAAAGGAAA CTCCAGCCAT
    CAGCAGGCGC AGCCCACTCG CCGTCAGTCT ACAGCATTTA GCATTTTCAA CACCCCCAGA
    AAGCTGGGCA ACAGCCTCCT GAAGAGAGGC CTCAACAAGA AAACGACTCC CAAAAATAGC
    CCCCGGGGGC GGGGGGCAAG TGGCAGCGCC GGCAGCACTT CAGGCAAATC CCCGCACCGC
    TCTCTCCGGA AGTCACCCAG CAGGCGGTCT CCGAGGGCCT CCACTGCAAA GTCCCCCAAA
    GCTTCAACCA AGGTGGGGCA GGAGCTGCAG ATACAGTTTT TCGAGCGCAA GCATCAGAGG
    AACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753277.1
    CDS138..7046
    Translation

    Target ORF information:

    RefSeq Version XM_031897417.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897417.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CAGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTGT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGTGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAAGAAAC AACTGGAACG CAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGGAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGGAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAA TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGAGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCG
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAA
    GAGCTGGAGG CGGCAAAGAA AGAGCAAAGT GAGCAGGTGC AGGAGCTGAA CAAGCAGCTG
    AGCCAGCAGG AGAAGGCCTT ACGGAGCCAG CAGCACAAGC TGAAGCAGCA GGAAGGTGAG
    ACTCATGAGG AGGCTGAGAA GGCTCAGAAG CGAGTGGTGG AGTTGGAGTC CCAGGTGGAA
    CAGCAGACTC AGGCCGTGGA ACATTACAAA GCTCAGATGG AGAAAGCCAA GCTGTATTAC
    GATGCCAAGA AGAAGCAGAA CCAGGAGCTA AGCGAGGAGC TGCAAAGCCA CATTAGAGAG
    CAGGAACATC TGCGCAAGGA GAACGTAGAT CTAAAGGCAG AGTCTGAGCA GCTCAGTAAG
    GAGCTACAGC ATTCGCTGCT ACAGAACAAA GAGGCAGAGC AAAACTGCAA GAACCTGTCC
    AATCGGGTTA GGAGTCTGGA GGCCCAGGTG GAGTATGCCG ACCGGCAGCT GCGAGACCTG
    GGGAAGTTCC AGTTGGCCAC GGATTCCATG AAGAGTCGGG AAACTTTCGG AGCCCCCCGT
    GTAACACGGA GCCATGCAGA TGTCAGTATA GACAGCCTGG ACTTGAGCTT TGAGGATGAC
    CAGCTTTTGA ACTCAACAAG TAAGAATGGC CGCTGCAACG AGGAACCTGC CACATCGTCA
    GTACATGCCA GCAGCATGGA GTCTCCTACC ACAGGCCAAC TGCCCAAAAA GGTGGAGTCT
    CTGGAAAGCC TCTACTTCAC ACCTATTCCA ACCCGGGCAC AGTCCAAACT GGAAAGTAGC
    CTTGGTTCCA TTGGGGACCT GTCCCTAGAC TCCAGCAAGA AAACGCGGTC TGCAAGGAGA
    CGCACCACCC AGATCATTAA CATCACAATG ACCAAGAAGA CAAAGGAGGA ACCGGAAGCA
    GAGAGTGCCA ACACGTCATT CTACAGCCTG CGCTCCGCTC CGTCCTATCA GAGTCTCCAC
    CAGCAGAAGC CCCGGAGGCC TGGGCGTCCT CAGGCAGCGG TTTCTGCCCC AGCCCTGGCC
    AGCCTCCCAT CCCAGGAATC CCTGGCCAAG ACAGAACAGT TCTCTGATGA CAGCTTTAAT
    AACTCCGTAT TGATGAATTT ACCTGGTTAC CGACCCCCCA CGCGTAGATC TGCAAGGCTT
    TCACAAACTG GAGGCCGAAG CAGTTTCTAC ATGAGCACGT GCCAGGATGA GCCAGACCCA
    CAGGAAGACT GGAACCGGAT CGCTGAGTTG CAGGCCCGGA ACAAGGCCTG CCCACCCCAC
    CTCAAGACTT CCTACCCACT GGAGTCCAGG CCGTCACTCT TAAGCTCCAC CATTACCGAT
    GAAGAGGTCA AGTTGGGAGA CCCTAAAGAG ACGCTCAGGA GAGCCACCAT GCTGCCCTCC
    CAGATACAAG AGTCCACAGG CAGTACCCGC AGAATGAGCT TGGCTGCCTC TGAACATATG
    AGGGGACCCA GCATCAGCAC CAGGCAGCAG ATGAAGCGCG TGTCCGAGGA GTCTCACCTT
    GGCCCAGACA CCCCAGAAGC CAAGAAGTCT GCCACTTGCT TTCCTCGGCC CATGACCCCA
    AAGGATAAAC ACGACGCACG CAGATTGTCC ACTGTGGAAA GCAAAGGAAA CTCCAGCCAT
    CAGCAGGCGC AGCCCACTCG CCGTCAGTCT ACAGCATTTA GCATTTTCAA CACCCCCAGA
    AAGCTGGGCA ACAGCCTCCT GAAGAGAGGC CTCAACAAGA AAACGACTCC CAAAAATAGC
    CCCCGGGGGC GGGGGGCAAG TGGCAGCGCC GGCAGCACTT CAGGCAAATC CCCGCACCGC
    TCTCTCCGGA AGTCACCCAG CAGGCGGTCT CCGAGGGCCT CCACTGCAAA GTCCCCCAAA
    GCTTCAACCA AGGTGGGGCA GGAGCTGCAG ATACAGTTTT TCGAGCGCAA GCATCAGAGG
    AACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53059
    Clone ID Related Accession (Same CDS sequence) XM_031897418.1
    Accession Version XM_031897418.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nuclear mitotic apparatus protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CAGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTGT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGTGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAAGAAAC AACTGGAACG CAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGGAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGGAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAA TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGAGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCG
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAA
    GAGCTGGAGG CGGCAAAGAA AGAGCAAAGT GAGCAGGTGC AGGAGCTGAA CAAGCAGCTG
    AGCCAGCAGG AGAAGGCCTT ACGGAGCCAG CAGCACAAGC TGAAGCAGCA GGAAGGTGAG
    ACTCATGAGG AGGCTGAGAA GGCTCAGAAG CGAGTGGTGG AGTTGGAGTC CCAGGTGGAA
    CAGCAGACTC AGGCCGTGGA ACATTACAAA GCTCAGATGG AGAAAGCCAA GCTGTATTAC
    GATGCCAAGA AGAAGCAGAA CCAGGAGCTA AGCGAGGAGC TGCAAAGCCA CATTAGAGAG
    CAGGAACATC TGCGCAAGGA GAACGTAGAT CTAAAGGCAG AGTCTGAGCA GCTCAGTAAG
    GAGCTACAGC ATTCGCTGCT ACAGAACAAA GAGGCAGAGC AAAACTGCAA GAACCTGTCC
    AATCGGGTTA GGAGTCTGGA GGCCCAGGTG GAGTATGCCG ACCGGCAGCT GCGAGACCTG
    GGGAAGTTCC AGTTGGCCAC GGATTCCATG AAGAGTCGGG AAACTTTCGG AGCCCCCCGT
    GTAACACGGA GCCATGCAGA TGTCAGTATA GACAGCCTGG ACTTGAGCTT TGAGGATGAC
    CAGCTTTTGA ACTCAACAAG TAAGAATGGC CGCTGCAACG AGGAACCTGC CACATCGTCA
    GTACATGCCA GCAGCATGGA GTCTCCTACC ACAGGCCAAC TGCCCAAAAA GGTGGAGTCT
    CTGGAAAGCC TCTACTTCAC ACCTATTCCA ACCCGGGCAC AGTCCAAACT GGAAAGTAGC
    CTTGGTTCCA TTGGGGACCT GTCCCTAGAC TCCAGCAAGA AAACGCGGTC TGCAAGGAGA
    CGCACCACCC AGATCATTAA CATCACAATG ACCAAGAAGA CAAAGGAGGA ACCGGAAGCA
    GAGAGTGCCA ACACGTCATT CTACAGCCTG CGCTCCGCTC CGTCCTATCA GAGTCTCCAC
    CAGCAGAAGC CCCGGAGGCC TGGGCGTCCT CAGGCAGCGG TTTCTGCCCC AGCCCTGGCC
    AGCCTCCCAT CCCAGGAATC CCTGGCCAAG ACAGAACAGT TCTCTGATGA CAGCTTTAAT
    AACTCCGTAT TGATGAATTT ACCTGGTTAC CGACCCCCCA CGCGTAGATC TGCAAGGCTT
    TCACAAACTG GAGGCCGAAG CAGTTTCTAC ATGAGCACGT GCCAGGATGA GCCAGACCCA
    CAGGAAGACT GGAACCGGAT CGCTGAGTTG CAGGCCCGGA ACAAGGCCTG CCCACCCCAC
    CTCAAGACTT CCTACCCACT GGAGTCCAGG CCGTCACTCT TAAGCTCCAC CATTACCGAT
    GAAGAGGTCA AGTTGGGAGA CCCTAAAGAG ACGCTCAGGA GAGCCACCAT GCTGCCCTCC
    CAGATACAAG AGTCCACAGG CAGTACCCGC AGAATGAGCT TGGCTGCCTC TGAACATATG
    AGGGGACCCA GCATCAGCAC CAGGCAGCAG ATGAAGCGCG TGTCCGAGGA GTCTCACCTT
    GGCCCAGACA CCCCAGAAGC CAAGAAGTCT GCCACTTGCT TTCCTCGGCC CATGACCCCA
    AAGGATAAAC ACGACGCACG CAGATTGTCC ACTGTGGAAA GCAAAGGAAA CTCCAGCCAT
    CAGGCGCAGC CCACTCGCCG TCAGTCTACA GCATTTAGCA TTTTCAACAC CCCCAGAAAG
    CTGGGCAACA GCCTCCTGAA GAGAGGCCTC AACAAGAAAA CGACTCCCAA AAATAGCCCC
    CGGGGGCGGG GGGCAAGTGG CAGCGCCGGC AGCACTTCAG GCAAATCCCC GCACCGCTCT
    CTCCGGAAGT CACCCAGCAG GCGGTCTCCG AGGGCCTCCA CTGCAAAGTC CCCCAAAGCT
    TCAACCAAGG TGGGGCAGGA GCTGCAGATA CAGTTTTTCG AGCGCAAGCA TCAGAGGAAC
    AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753278.1
    CDS132..7037
    Translation

    Target ORF information:

    RefSeq Version XM_031897418.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897418.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CAGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTGT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGTGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAAGAAAC AACTGGAACG CAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGGAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGGAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAA TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGAGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCG
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAA
    GAGCTGGAGG CGGCAAAGAA AGAGCAAAGT GAGCAGGTGC AGGAGCTGAA CAAGCAGCTG
    AGCCAGCAGG AGAAGGCCTT ACGGAGCCAG CAGCACAAGC TGAAGCAGCA GGAAGGTGAG
    ACTCATGAGG AGGCTGAGAA GGCTCAGAAG CGAGTGGTGG AGTTGGAGTC CCAGGTGGAA
    CAGCAGACTC AGGCCGTGGA ACATTACAAA GCTCAGATGG AGAAAGCCAA GCTGTATTAC
    GATGCCAAGA AGAAGCAGAA CCAGGAGCTA AGCGAGGAGC TGCAAAGCCA CATTAGAGAG
    CAGGAACATC TGCGCAAGGA GAACGTAGAT CTAAAGGCAG AGTCTGAGCA GCTCAGTAAG
    GAGCTACAGC ATTCGCTGCT ACAGAACAAA GAGGCAGAGC AAAACTGCAA GAACCTGTCC
    AATCGGGTTA GGAGTCTGGA GGCCCAGGTG GAGTATGCCG ACCGGCAGCT GCGAGACCTG
    GGGAAGTTCC AGTTGGCCAC GGATTCCATG AAGAGTCGGG AAACTTTCGG AGCCCCCCGT
    GTAACACGGA GCCATGCAGA TGTCAGTATA GACAGCCTGG ACTTGAGCTT TGAGGATGAC
    CAGCTTTTGA ACTCAACAAG TAAGAATGGC CGCTGCAACG AGGAACCTGC CACATCGTCA
    GTACATGCCA GCAGCATGGA GTCTCCTACC ACAGGCCAAC TGCCCAAAAA GGTGGAGTCT
    CTGGAAAGCC TCTACTTCAC ACCTATTCCA ACCCGGGCAC AGTCCAAACT GGAAAGTAGC
    CTTGGTTCCA TTGGGGACCT GTCCCTAGAC TCCAGCAAGA AAACGCGGTC TGCAAGGAGA
    CGCACCACCC AGATCATTAA CATCACAATG ACCAAGAAGA CAAAGGAGGA ACCGGAAGCA
    GAGAGTGCCA ACACGTCATT CTACAGCCTG CGCTCCGCTC CGTCCTATCA GAGTCTCCAC
    CAGCAGAAGC CCCGGAGGCC TGGGCGTCCT CAGGCAGCGG TTTCTGCCCC AGCCCTGGCC
    AGCCTCCCAT CCCAGGAATC CCTGGCCAAG ACAGAACAGT TCTCTGATGA CAGCTTTAAT
    AACTCCGTAT TGATGAATTT ACCTGGTTAC CGACCCCCCA CGCGTAGATC TGCAAGGCTT
    TCACAAACTG GAGGCCGAAG CAGTTTCTAC ATGAGCACGT GCCAGGATGA GCCAGACCCA
    CAGGAAGACT GGAACCGGAT CGCTGAGTTG CAGGCCCGGA ACAAGGCCTG CCCACCCCAC
    CTCAAGACTT CCTACCCACT GGAGTCCAGG CCGTCACTCT TAAGCTCCAC CATTACCGAT
    GAAGAGGTCA AGTTGGGAGA CCCTAAAGAG ACGCTCAGGA GAGCCACCAT GCTGCCCTCC
    CAGATACAAG AGTCCACAGG CAGTACCCGC AGAATGAGCT TGGCTGCCTC TGAACATATG
    AGGGGACCCA GCATCAGCAC CAGGCAGCAG ATGAAGCGCG TGTCCGAGGA GTCTCACCTT
    GGCCCAGACA CCCCAGAAGC CAAGAAGTCT GCCACTTGCT TTCCTCGGCC CATGACCCCA
    AAGGATAAAC ACGACGCACG CAGATTGTCC ACTGTGGAAA GCAAAGGAAA CTCCAGCCAT
    CAGGCGCAGC CCACTCGCCG TCAGTCTACA GCATTTAGCA TTTTCAACAC CCCCAGAAAG
    CTGGGCAACA GCCTCCTGAA GAGAGGCCTC AACAAGAAAA CGACTCCCAA AAATAGCCCC
    CGGGGGCGGG GGGCAAGTGG CAGCGCCGGC AGCACTTCAG GCAAATCCCC GCACCGCTCT
    CTCCGGAAGT CACCCAGCAG GCGGTCTCCG AGGGCCTCCA CTGCAAAGTC CCCCAAAGCT
    TCAACCAAGG TGGGGCAGGA GCTGCAGATA CAGTTTTTCG AGCGCAAGCA TCAGAGGAAC
    AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53060
    Clone ID Related Accession (Same CDS sequence) XM_031897419.1
    Accession Version XM_031897419.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nuclear mitotic apparatus protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CAGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTGT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGTGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAAGAAAC AACTGGAACG CAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGGAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGGAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAA TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGAGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCG
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAA
    GAGCTGGAGG CGGCAAAGAA AGAGCAAAGT GAGCAGGTGC AGGAGCTGAA CAAGCAGCTG
    AGCCAGCAGG AGAAGGCCTT ACGGAGCCAG CAGCACAAGC TGAAGCAGCA GGAAGGTGAG
    ACTCATGAGG AGGCTGAGAA GGCTCAGAAG CGAGTGGTGG AGTTGGAGTC CCAGGTGGAA
    CAGCAGACTC AGGCCGTGGA ACATTACAAA GCTCAGATGG AGAAAGCCAA GCTGTATTAC
    GATGCCAAGA AGAAGCAGAA CCAGGAGCTA AGCGAGGAGC TGCAAAGCCA CATTAGAGAG
    CAGGAACATC TGCGCAAGGA GAACGTAGAT CTAAAGGCAG AGTCTGAGCA GCTCAGTAAG
    GAGCTACAGC ATTCGCTGCT ACAGAACAAA GAGGCAGAGC AAAACTGCAA GAACCTGTCC
    AATCGGGTTA GGAGTCTGGA GGCCCAGGTG GAGTATGCCG ACCGGCAGCT GCGAGACCTG
    GGGAAGTTCC AGTTGGCCAC GGATTCCATG AAGAGTCGGG AAACTTTCGG AGCCCCCCGT
    GTAACACGGA GCCATGCAGA TGTCAGTATA GACAGCCTGG ACTTGAGCTT TGAGGATGAC
    CAGCTTTTGA ACTCAACAAG TAAGAATGGC CGCTGCAACG AGGAACCTGC CACATCGTCA
    GTACATGCCA GCAGCATGGA GTCTCCTACC ACAGGCCAAC TGCCCAAAAA GGTGGAGTCT
    CTGGAAAGCC TCTACTTCAC ACCTATTCCA ACCCGGGCAC AGTCCAAACT GGAAAGTAGC
    CTTGGTTCCA TTGGGGACCT GTCCCTAGAC TCCAGCAAGA AAACGCGGTC TGCAAGGAGA
    CGCACCACCC AGATCATTAA CATCACAATG ACCAAGAAGA CAAAGGAGGA ACCGGAAGCA
    GAGAGTGCCA ACACGTCATT CTACAGCCTG CGCTCCGCTC CGTCCTATCA GAGTCTCCAC
    CAGCAGAAGC CCCGGAGGCC TGGGCGTCCT CAGGCAGCGG TTTCTGCCCC AGCCCTGGCC
    AGCCTCCCAT CCCAGGAATC CCTGGCCAAG ACAGAACAGT TCTCTGATGA CAGCTTTAAT
    AACTCCGTAT TGATGAATTT ACCTGGTTAC CGACCCCCCA CGCGTAGATC TGCAAGGCTT
    TCACAAACTG GAGGCCGAAG CAGTTTCTAC ATGAGCACGT GCCAGGATGA GCCAGACCCA
    CAGGAAGACT GGAACCGGAT CGCTGAGTTG CAGGCCCGGA ACAAGGCCTG CCCACCCCAC
    CTCAAGACTT CCTACCCACT GGAGTCCAGG CCGTCACTCT TAAGCTCCAC CATTACCGAT
    GAAGAGGTCA AGTTGGGAGA CCCTAAAGAG ACGCTCAGGA GAGCCACCAT GCTGCCCTCC
    CAGATACAAG AGTCCACAGG CAGTACCCGC AGAATGAGCT TGGCTGCCTC TGAACATATG
    AGGGGACCCA GCATCAGCAC CAGGCAGCAG ATGAAGCGCG TGTCCGAGGA GTCTCACCTT
    GGCCCAGACA CCCCAGAAGC CAAGAAGTCT GCCACTTGCT TTCCTCGGCC CATGACCCCA
    AAGGATAAAC ACGACGCACG CAGATTGTCC ACTGTGGAAA GCAAAGGAAA CTCCAGCCAT
    CAGCAGGCGC AGCCCACTCG CCGTCAGTCT ACAGCATTTA GCATTTTCAA CACCCCCAGA
    AAGCTGGGCA ACAGCCTCCT GAAGAGAGGC CTCAACAAGA AAACGACTCC CAAAAATAGC
    CCCCGGGGGC GGGGGGCAAG TGGCAGCGCC GGCAGCACTT CAGGCAAATC CCCGCACCGC
    TCTCTCCGGA AGTCACCCAG CAGGCGGTCT CCGAGGGCCT CCACTGCAAA GTCCCCCAAA
    GCTTCAACCA AGTTTTTCGA GCGCAAGCAT CAGAGGAACA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753279.1
    CDS132..7016
    Translation

    Target ORF information:

    RefSeq Version XM_031897419.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897419.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CAGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTGT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGTGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAAGAAAC AACTGGAACG CAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGGAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGGAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAA TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGAGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCG
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAA
    GAGCTGGAGG CGGCAAAGAA AGAGCAAAGT GAGCAGGTGC AGGAGCTGAA CAAGCAGCTG
    AGCCAGCAGG AGAAGGCCTT ACGGAGCCAG CAGCACAAGC TGAAGCAGCA GGAAGGTGAG
    ACTCATGAGG AGGCTGAGAA GGCTCAGAAG CGAGTGGTGG AGTTGGAGTC CCAGGTGGAA
    CAGCAGACTC AGGCCGTGGA ACATTACAAA GCTCAGATGG AGAAAGCCAA GCTGTATTAC
    GATGCCAAGA AGAAGCAGAA CCAGGAGCTA AGCGAGGAGC TGCAAAGCCA CATTAGAGAG
    CAGGAACATC TGCGCAAGGA GAACGTAGAT CTAAAGGCAG AGTCTGAGCA GCTCAGTAAG
    GAGCTACAGC ATTCGCTGCT ACAGAACAAA GAGGCAGAGC AAAACTGCAA GAACCTGTCC
    AATCGGGTTA GGAGTCTGGA GGCCCAGGTG GAGTATGCCG ACCGGCAGCT GCGAGACCTG
    GGGAAGTTCC AGTTGGCCAC GGATTCCATG AAGAGTCGGG AAACTTTCGG AGCCCCCCGT
    GTAACACGGA GCCATGCAGA TGTCAGTATA GACAGCCTGG ACTTGAGCTT TGAGGATGAC
    CAGCTTTTGA ACTCAACAAG TAAGAATGGC CGCTGCAACG AGGAACCTGC CACATCGTCA
    GTACATGCCA GCAGCATGGA GTCTCCTACC ACAGGCCAAC TGCCCAAAAA GGTGGAGTCT
    CTGGAAAGCC TCTACTTCAC ACCTATTCCA ACCCGGGCAC AGTCCAAACT GGAAAGTAGC
    CTTGGTTCCA TTGGGGACCT GTCCCTAGAC TCCAGCAAGA AAACGCGGTC TGCAAGGAGA
    CGCACCACCC AGATCATTAA CATCACAATG ACCAAGAAGA CAAAGGAGGA ACCGGAAGCA
    GAGAGTGCCA ACACGTCATT CTACAGCCTG CGCTCCGCTC CGTCCTATCA GAGTCTCCAC
    CAGCAGAAGC CCCGGAGGCC TGGGCGTCCT CAGGCAGCGG TTTCTGCCCC AGCCCTGGCC
    AGCCTCCCAT CCCAGGAATC CCTGGCCAAG ACAGAACAGT TCTCTGATGA CAGCTTTAAT
    AACTCCGTAT TGATGAATTT ACCTGGTTAC CGACCCCCCA CGCGTAGATC TGCAAGGCTT
    TCACAAACTG GAGGCCGAAG CAGTTTCTAC ATGAGCACGT GCCAGGATGA GCCAGACCCA
    CAGGAAGACT GGAACCGGAT CGCTGAGTTG CAGGCCCGGA ACAAGGCCTG CCCACCCCAC
    CTCAAGACTT CCTACCCACT GGAGTCCAGG CCGTCACTCT TAAGCTCCAC CATTACCGAT
    GAAGAGGTCA AGTTGGGAGA CCCTAAAGAG ACGCTCAGGA GAGCCACCAT GCTGCCCTCC
    CAGATACAAG AGTCCACAGG CAGTACCCGC AGAATGAGCT TGGCTGCCTC TGAACATATG
    AGGGGACCCA GCATCAGCAC CAGGCAGCAG ATGAAGCGCG TGTCCGAGGA GTCTCACCTT
    GGCCCAGACA CCCCAGAAGC CAAGAAGTCT GCCACTTGCT TTCCTCGGCC CATGACCCCA
    AAGGATAAAC ACGACGCACG CAGATTGTCC ACTGTGGAAA GCAAAGGAAA CTCCAGCCAT
    CAGCAGGCGC AGCCCACTCG CCGTCAGTCT ACAGCATTTA GCATTTTCAA CACCCCCAGA
    AAGCTGGGCA ACAGCCTCCT GAAGAGAGGC CTCAACAAGA AAACGACTCC CAAAAATAGC
    CCCCGGGGGC GGGGGGCAAG TGGCAGCGCC GGCAGCACTT CAGGCAAATC CCCGCACCGC
    TCTCTCCGGA AGTCACCCAG CAGGCGGTCT CCGAGGGCCT CCACTGCAAA GTCCCCCAAA
    GCTTCAACCA AGTTTTTCGA GCGCAAGCAT CAGAGGAACA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53061
    Clone ID Related Accession (Same CDS sequence) XM_031897420.1
    Accession Version XM_031897420.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nuclear mitotic apparatus protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CAGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTGT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGTGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAAGAAAC AACTGGAACG CAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGGAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGGAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAA TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGAGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCG
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAG
    GAGCTGAACA AGCAGCTGAG CCAGCAGGAG AAGGCCTTAC GGAGCCAGCA GCACAAGCTG
    AAGCAGCAGG AAGGTGAGAC TCATGAGGAG GCTGAGAAGG CTCAGAAGCG AGTGGTGGAG
    TTGGAGTCCC AGGTGGAACA GCAGACTCAG GCCGTGGAAC ATTACAAAGC TCAGATGGAG
    AAAGCCAAGC TGTATTACGA TGCCAAGAAG AAGCAGAACC AGGAGCTAAG CGAGGAGCTG
    CAAAGCCACA TTAGAGAGCA GGAACATCTG CGCAAGGAGA ACGTAGATCT AAAGGCAGAG
    TCTGAGCAGC TCAGTAAGGA GCTACAGCAT TCGCTGCTAC AGAACAAAGA GGCAGAGCAA
    AACTGCAAGA ACCTGTCCAA TCGGGTTAGG AGTCTGGAGG CCCAGGTGGA GTATGCCGAC
    CGGCAGCTGC GAGACCTGGG GAAGTTCCAG TTGGCCACGG ATTCCATGAA GAGTCGGGAA
    ACTTTCGGAG CCCCCCGTGT AACACGGAGC CATGCAGATG TCAGTATAGA CAGCCTGGAC
    TTGAGCTTTG AGGATGACCA GCTTTTGAAC TCAACAAGTA AGAATGGCCG CTGCAACGAG
    GAACCTGCCA CATCGTCAGT ACATGCCAGC AGCATGGAGT CTCCTACCAC AGGCCAACTG
    CCCAAAAAGG TGGAGTCTCT GGAAAGCCTC TACTTCACAC CTATTCCAAC CCGGGCACAG
    TCCAAACTGG AAAGTAGCCT TGGTTCCATT GGGGACCTGT CCCTAGACTC CAGCAAGAAA
    ACGCGGTCTG CAAGGAGACG CACCACCCAG ATCATTAACA TCACAATGAC CAAGAAGACA
    AAGGAGGAAC CGGAAGCAGA GAGTGCCAAC ACGTCATTCT ACAGCCTGCG CTCCGCTCCG
    TCCTATCAGA GTCTCCACCA GCAGAAGCCC CGGAGGCCTG GGCGTCCTCA GGCAGCGGTT
    TCTGCCCCAG CCCTGGCCAG CCTCCCATCC CAGGAATCCC TGGCCAAGAC AGAACAGTTC
    TCTGATGACA GCTTTAATAA CTCCGTATTG ATGAATTTAC CTGGTTACCG ACCCCCCACG
    CGTAGATCTG CAAGGCTTTC ACAAACTGGA GGCCGAAGCA GTTTCTACAT GAGCACGTGC
    CAGGATGAGC CAGACCCACA GGAAGACTGG AACCGGATCG CTGAGTTGCA GGCCCGGAAC
    AAGGCCTGCC CACCCCACCT CAAGACTTCC TACCCACTGG AGTCCAGGCC GTCACTCTTA
    AGCTCCACCA TTACCGATGA AGAGGTCAAG TTGGGAGACC CTAAAGAGAC GCTCAGGAGA
    GCCACCATGC TGCCCTCCCA GATACAAGAG TCCACAGGCA GTACCCGCAG AATGAGCTTG
    GCTGCCTCTG AACATATGAG GGGACCCAGC ATCAGCACCA GGCAGCAGAT GAAGCGCGTG
    TCCGAGGAGT CTCACCTTGG CCCAGACACC CCAGAAGCCA AGAAGTCTGC CACTTGCTTT
    CCTCGGCCCA TGACCCCAAA GGATAAACAC GACGCACGCA GATTGTCCAC TGTGGAAAGC
    AAAGGAAACT CCAGCCATCA GCAGGCGCAG CCCACTCGCC GTCAGTCTAC AGCATTTAGC
    ATTTTCAACA CCCCCAGAAA GCTGGGCAAC AGCCTCCTGA AGAGAGGCCT CAACAAGAAA
    ACGACTCCCA AAAATAGCCC CCGGGGGCGG GGGGCAAGTG GCAGCGCCGG CAGCACTTCA
    GGCAAATCCC CGCACCGCTC TCTCCGGAAG TCACCCAGCA GGCGGTCTCC GAGGGCCTCC
    ACTGCAAAGT CCCCCAAAGC TTCAACCAAG GTGGGGCAGG AGCTGCAGAT ACAGTTTTTC
    GAGCGCAAGC ATCAGAGGAA CAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753280.1
    CDS132..6998
    Translation

    Target ORF information:

    RefSeq Version XM_031897420.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897420.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CAGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTGT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGTGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAAGAAAC AACTGGAACG CAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGGAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGGAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAA TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGAGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCG
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAG
    GAGCTGAACA AGCAGCTGAG CCAGCAGGAG AAGGCCTTAC GGAGCCAGCA GCACAAGCTG
    AAGCAGCAGG AAGGTGAGAC TCATGAGGAG GCTGAGAAGG CTCAGAAGCG AGTGGTGGAG
    TTGGAGTCCC AGGTGGAACA GCAGACTCAG GCCGTGGAAC ATTACAAAGC TCAGATGGAG
    AAAGCCAAGC TGTATTACGA TGCCAAGAAG AAGCAGAACC AGGAGCTAAG CGAGGAGCTG
    CAAAGCCACA TTAGAGAGCA GGAACATCTG CGCAAGGAGA ACGTAGATCT AAAGGCAGAG
    TCTGAGCAGC TCAGTAAGGA GCTACAGCAT TCGCTGCTAC AGAACAAAGA GGCAGAGCAA
    AACTGCAAGA ACCTGTCCAA TCGGGTTAGG AGTCTGGAGG CCCAGGTGGA GTATGCCGAC
    CGGCAGCTGC GAGACCTGGG GAAGTTCCAG TTGGCCACGG ATTCCATGAA GAGTCGGGAA
    ACTTTCGGAG CCCCCCGTGT AACACGGAGC CATGCAGATG TCAGTATAGA CAGCCTGGAC
    TTGAGCTTTG AGGATGACCA GCTTTTGAAC TCAACAAGTA AGAATGGCCG CTGCAACGAG
    GAACCTGCCA CATCGTCAGT ACATGCCAGC AGCATGGAGT CTCCTACCAC AGGCCAACTG
    CCCAAAAAGG TGGAGTCTCT GGAAAGCCTC TACTTCACAC CTATTCCAAC CCGGGCACAG
    TCCAAACTGG AAAGTAGCCT TGGTTCCATT GGGGACCTGT CCCTAGACTC CAGCAAGAAA
    ACGCGGTCTG CAAGGAGACG CACCACCCAG ATCATTAACA TCACAATGAC CAAGAAGACA
    AAGGAGGAAC CGGAAGCAGA GAGTGCCAAC ACGTCATTCT ACAGCCTGCG CTCCGCTCCG
    TCCTATCAGA GTCTCCACCA GCAGAAGCCC CGGAGGCCTG GGCGTCCTCA GGCAGCGGTT
    TCTGCCCCAG CCCTGGCCAG CCTCCCATCC CAGGAATCCC TGGCCAAGAC AGAACAGTTC
    TCTGATGACA GCTTTAATAA CTCCGTATTG ATGAATTTAC CTGGTTACCG ACCCCCCACG
    CGTAGATCTG CAAGGCTTTC ACAAACTGGA GGCCGAAGCA GTTTCTACAT GAGCACGTGC
    CAGGATGAGC CAGACCCACA GGAAGACTGG AACCGGATCG CTGAGTTGCA GGCCCGGAAC
    AAGGCCTGCC CACCCCACCT CAAGACTTCC TACCCACTGG AGTCCAGGCC GTCACTCTTA
    AGCTCCACCA TTACCGATGA AGAGGTCAAG TTGGGAGACC CTAAAGAGAC GCTCAGGAGA
    GCCACCATGC TGCCCTCCCA GATACAAGAG TCCACAGGCA GTACCCGCAG AATGAGCTTG
    GCTGCCTCTG AACATATGAG GGGACCCAGC ATCAGCACCA GGCAGCAGAT GAAGCGCGTG
    TCCGAGGAGT CTCACCTTGG CCCAGACACC CCAGAAGCCA AGAAGTCTGC CACTTGCTTT
    CCTCGGCCCA TGACCCCAAA GGATAAACAC GACGCACGCA GATTGTCCAC TGTGGAAAGC
    AAAGGAAACT CCAGCCATCA GCAGGCGCAG CCCACTCGCC GTCAGTCTAC AGCATTTAGC
    ATTTTCAACA CCCCCAGAAA GCTGGGCAAC AGCCTCCTGA AGAGAGGCCT CAACAAGAAA
    ACGACTCCCA AAAATAGCCC CCGGGGGCGG GGGGCAAGTG GCAGCGCCGG CAGCACTTCA
    GGCAAATCCC CGCACCGCTC TCTCCGGAAG TCACCCAGCA GGCGGTCTCC GAGGGCCTCC
    ACTGCAAAGT CCCCCAAAGC TTCAACCAAG GTGGGGCAGG AGCTGCAGAT ACAGTTTTTC
    GAGCGCAAGC ATCAGAGGAA CAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53062
    Clone ID Related Accession (Same CDS sequence) XM_031897421.1
    Accession Version XM_031897421.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nuclear mitotic apparatus protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CAGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTGT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGTGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAAGAAAC AACTGGAACG CAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGGAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGGAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAA TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGAGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCG
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAG
    GAGCTGAACA AGCAGCTGAG CCAGCAGGAG AAGGCCTTAC GGAGCCAGCA GCACAAGCTG
    AAGCAGCAGG AAGGTGAGAC TCATGAGGAG GCTGAGAAGG CTCAGAAGCG AGTGGTGGAG
    TTGGAGTCCC AGGTGGAACA GCAGACTCAG GCCGTGGAAC ATTACAAAGC TCAGATGGAG
    AAAGCCAAGC TGTATTACGA TGCCAAGAAG AAGCAGAACC AGGAGCTAAG CGAGGAGCTG
    CAAAGCCACA TTAGAGAGCA GGAACATCTG CGCAAGGAGA ACGTAGATCT AAAGGCAGAG
    TCTGAGCAGC TCAGTAAGGA GCTACAGCAT TCGCTGCTAC AGAACAAAGA GGCAGAGCAA
    AACTGCAAGA ACCTGTCCAA TCGGGTTAGG AGTCTGGAGG CCCAGGTGGA GTATGCCGAC
    CGGCAGCTGC GAGACCTGGG GAAGTTCCAG TTGGCCACGG ATTCCATGAA GAGTCGGGAA
    ACTTTCGGAG CCCCCCGTGT AACACGGAGC CATGCAGATG TCAGTATAGA CAGCCTGGAC
    TTGAGCTTTG AGGATGACCA GCTTTTGAAC TCAACAAGTA AGAATGGCCG CTGCAACGAG
    GAACCTGCCA CATCGTCAGT ACATGCCAGC AGCATGGAGT CTCCTACCAC AGGCCAACTG
    CCCAAAAAGG TGGAGTCTCT GGAAAGCCTC TACTTCACAC CTATTCCAAC CCGGGCACAG
    TCCAAACTGG AAAGTAGCCT TGGTTCCATT GGGGACCTGT CCCTAGACTC CAGCAAGAAA
    ACGCGGTCTG CAAGGAGACG CACCACCCAG ATCATTAACA TCACAATGAC CAAGAAGACA
    AAGGAGGAAC CGGAAGCAGA GAGTGCCAAC ACGTCATTCT ACAGCCTGCG CTCCGCTCCG
    TCCTATCAGA GTCTCCACCA GCAGAAGCCC CGGAGGCCTG GGCGTCCTCA GGCAGCGGTT
    TCTGCCCCAG CCCTGGCCAG CCTCCCATCC CAGGAATCCC TGGCCAAGAC AGAACAGTTC
    TCTGATGACA GCTTTAATAA CTCCGTATTG ATGAATTTAC CTGGTTACCG ACCCCCCACG
    CGTAGATCTG CAAGGCTTTC ACAAACTGGA GGCCGAAGCA GTTTCTACAT GAGCACGTGC
    CAGGATGAGC CAGACCCACA GGAAGACTGG AACCGGATCG CTGAGTTGCA GGCCCGGAAC
    AAGGCCTGCC CACCCCACCT CAAGACTTCC TACCCACTGG AGTCCAGGCC GTCACTCTTA
    AGCTCCACCA TTACCGATGA AGAGGTCAAG TTGGGAGACC CTAAAGAGAC GCTCAGGAGA
    GCCACCATGC TGCCCTCCCA GATACAAGAG TCCACAGGCA GTACCCGCAG AATGAGCTTG
    GCTGCCTCTG AACATATGAG GGGACCCAGC ATCAGCACCA GGCAGCAGAT GAAGCGCGTG
    TCCGAGGAGT CTCACCTTGG CCCAGACACC CCAGAAGCCA AGAAGTCTGC CACTTGCTTT
    CCTCGGCCCA TGACCCCAAA GGATAAACAC GACGCACGCA GATTGTCCAC TGTGGAAAGC
    AAAGGAAACT CCAGCCATCA GCAGGCGCAG CCCACTCGCC GTCAGTCTAC AGCATTTAGC
    ATTTTCAACA CCCCCAGAAA GCTGGGCAAC AGCCTCCTGA AGAGAGGCCT CAACAAGAAA
    ACGACTCCCA AAAATAGCCC CCGGGGGCGG GGGGCAAGTG GCAGCGCCGG CAGCACTTCA
    GGCAAATCCC CGCACCGCTC TCTCCGGAAG TCACCCAGCA GGCGGTCTCC GAGGGCCTCC
    ACTGCAAAGT CCCCCAAAGC TTCAACCAAG TTTTTCGAGC GCAAGCATCA GAGGAACAAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753281.1
    CDS132..6974
    Translation

    Target ORF information:

    RefSeq Version XM_031897421.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897421.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CAGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTGT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGTGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAAGAAAC AACTGGAACG CAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGGAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGGAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAA TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGAGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCG
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAG
    GAGCTGAACA AGCAGCTGAG CCAGCAGGAG AAGGCCTTAC GGAGCCAGCA GCACAAGCTG
    AAGCAGCAGG AAGGTGAGAC TCATGAGGAG GCTGAGAAGG CTCAGAAGCG AGTGGTGGAG
    TTGGAGTCCC AGGTGGAACA GCAGACTCAG GCCGTGGAAC ATTACAAAGC TCAGATGGAG
    AAAGCCAAGC TGTATTACGA TGCCAAGAAG AAGCAGAACC AGGAGCTAAG CGAGGAGCTG
    CAAAGCCACA TTAGAGAGCA GGAACATCTG CGCAAGGAGA ACGTAGATCT AAAGGCAGAG
    TCTGAGCAGC TCAGTAAGGA GCTACAGCAT TCGCTGCTAC AGAACAAAGA GGCAGAGCAA
    AACTGCAAGA ACCTGTCCAA TCGGGTTAGG AGTCTGGAGG CCCAGGTGGA GTATGCCGAC
    CGGCAGCTGC GAGACCTGGG GAAGTTCCAG TTGGCCACGG ATTCCATGAA GAGTCGGGAA
    ACTTTCGGAG CCCCCCGTGT AACACGGAGC CATGCAGATG TCAGTATAGA CAGCCTGGAC
    TTGAGCTTTG AGGATGACCA GCTTTTGAAC TCAACAAGTA AGAATGGCCG CTGCAACGAG
    GAACCTGCCA CATCGTCAGT ACATGCCAGC AGCATGGAGT CTCCTACCAC AGGCCAACTG
    CCCAAAAAGG TGGAGTCTCT GGAAAGCCTC TACTTCACAC CTATTCCAAC CCGGGCACAG
    TCCAAACTGG AAAGTAGCCT TGGTTCCATT GGGGACCTGT CCCTAGACTC CAGCAAGAAA
    ACGCGGTCTG CAAGGAGACG CACCACCCAG ATCATTAACA TCACAATGAC CAAGAAGACA
    AAGGAGGAAC CGGAAGCAGA GAGTGCCAAC ACGTCATTCT ACAGCCTGCG CTCCGCTCCG
    TCCTATCAGA GTCTCCACCA GCAGAAGCCC CGGAGGCCTG GGCGTCCTCA GGCAGCGGTT
    TCTGCCCCAG CCCTGGCCAG CCTCCCATCC CAGGAATCCC TGGCCAAGAC AGAACAGTTC
    TCTGATGACA GCTTTAATAA CTCCGTATTG ATGAATTTAC CTGGTTACCG ACCCCCCACG
    CGTAGATCTG CAAGGCTTTC ACAAACTGGA GGCCGAAGCA GTTTCTACAT GAGCACGTGC
    CAGGATGAGC CAGACCCACA GGAAGACTGG AACCGGATCG CTGAGTTGCA GGCCCGGAAC
    AAGGCCTGCC CACCCCACCT CAAGACTTCC TACCCACTGG AGTCCAGGCC GTCACTCTTA
    AGCTCCACCA TTACCGATGA AGAGGTCAAG TTGGGAGACC CTAAAGAGAC GCTCAGGAGA
    GCCACCATGC TGCCCTCCCA GATACAAGAG TCCACAGGCA GTACCCGCAG AATGAGCTTG
    GCTGCCTCTG AACATATGAG GGGACCCAGC ATCAGCACCA GGCAGCAGAT GAAGCGCGTG
    TCCGAGGAGT CTCACCTTGG CCCAGACACC CCAGAAGCCA AGAAGTCTGC CACTTGCTTT
    CCTCGGCCCA TGACCCCAAA GGATAAACAC GACGCACGCA GATTGTCCAC TGTGGAAAGC
    AAAGGAAACT CCAGCCATCA GCAGGCGCAG CCCACTCGCC GTCAGTCTAC AGCATTTAGC
    ATTTTCAACA CCCCCAGAAA GCTGGGCAAC AGCCTCCTGA AGAGAGGCCT CAACAAGAAA
    ACGACTCCCA AAAATAGCCC CCGGGGGCGG GGGGCAAGTG GCAGCGCCGG CAGCACTTCA
    GGCAAATCCC CGCACCGCTC TCTCCGGAAG TCACCCAGCA GGCGGTCTCC GAGGGCCTCC
    ACTGCAAAGT CCCCCAAAGC TTCAACCAAG TTTTTCGAGC GCAAGCATCA GAGGAACAAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa19633
    Clone ID Related Accession (Same CDS sequence) XM_004920135.3
    Accession Version XM_004920135.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nuclear mitotic apparatus protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683952.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004920135.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CGGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTAT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGGGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAGGAAAC AACTGGAACG AAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGCAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGCAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAG TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGGGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCA
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAA
    GAGCTGGAGG CGGCAAAGAA AGAGCAAAGT GAGCAGGTGC AGGAGCTGAA CAAGCAGCTG
    AGCCAGCAGG AGAAGGCCTT ACGGAGCCAG CAGCACAAGC TGAAGCAGCA GGAAGGTGAG
    ACTCATGAGG AGGCTGAGAA GGCTCAGAAG CGAGTGGTGG AGTTGGAGTC CCAGGTGGAA
    CAGCAGACTC AGGCCGTGGA ACATTACAAA GCTCAGATGG AGAAAGCCAA GCTGTATTAC
    GATGCCAAGA AGAAGCAGAA CCAGGAGCTA AGCGAGGAGC TGCAAAGCCA CATTAGAGAG
    CAGGAACATC TGCGCAAGGA GAACGTAGAT CTAAAGGCAG AGTCTGAGCA GCTCAGTAAG
    GAGCTACAGC ATTCGCTGCT ACAGAACAAA GAGGCAGAGC AAAACTGCAA GAACCTGTCC
    AATCGGGTTA GGAGTCTGGA GGCCCAGGTG GAGTATGCCG ACCGGCAGCT GCGAGACCTG
    GGGAAGTTCC AGTTGGCCAC GGATTCCATG AAGAGTCGGG AAACTTTCGG AGCCCCCCGT
    GTAACACGGA GCCATGCAGA TGTCAGTATA GACAGCCTGG ACTTGAGCTT TGAGGATGAC
    CAGCTTTTGA ACTCAACAAG TAAGAATGGC CGCTGCAACG AGGAACCTGC CACATCGTCA
    GTACATGCCA GCAGCATGGA GTCTCCTACC ACAGGCCAAC TGCCCAAAAA GGTGGAGTCT
    CTGGAAAGCC TCTACTTCAC ACCTATTCCA ACCCGGGCAC AGTCCAAACT GGAAAGTAGC
    CTTGGTTCCA TCGGGGACCT GTCCCTAGAC TCCAGCAAGA AAACGCGGTC TGCAAGGAGA
    CGCACCACCC AGATCATTAA CATCACAATG ACCAAGAAGA CGAAGGAGGA ACCGGAAGCA
    GAGAGTGCCA ACACGTCATT CTACAGCCTG CGCTCCGCTC CGTCCTATCA GAGTCTCCAC
    CAGCAGAAGC CCCGGAGGCC TGGGCGTCCT CAGGCAGCGG TTTCTGCCCC AGCCCTGGCC
    AGCCTCCCAT CCCAGGAATC CCTGGCCAAG ACAGAACAGT TCTCTGATGA CAGCTTTAAT
    AACTCCGTAT TGATGAATTT ACCTGGTTAC CGACCCCCCG CGCGTAGATC TGCAAGGCTT
    TCACAAACTG GAGGCCGAAG CAGTTTCTAC ATGAGCACGT GCCAGGATGA GCCAGACCCA
    CAGGAAGACT GGAACCGGAT CGCTGAGTTG CAGGCCCGGA ACAAGGCCTG CCCACCCCAC
    CTCAAGACTT CCTACCCACT GGAGTCCAGG CCGTCACTCT TAAGCTCCAC CATTACCGAT
    GAAGAGGTCA AGTTGGGAGA CCCTAAAGAG ACGCTCAGGA GAGCCACCAT GCTGCCCTCC
    CAGATACAAG AGTCCACAGG CAGTACCCGC AGAATGAGCT TGGCTGCCTC TGAACATATG
    AGGGGACCCA GCATCAGCAC CAGGCAGCAG ATGAAGCGCG TGTCCGAGGA GTCTCACCTT
    GGCCCAGACA CCCCAGAAGC CAAGAAGTCT GCCACTTGCT TTCCTCGGCC CATGACCCCA
    AAGGATAAAC ACGACGCACG CAGATTGTCC ACTGTGGAAA GCAAAGGAAA CTCCAGCCAT
    CAGCAGGCGC AGCCCACTCG CCGTCAGTCT ACAGCATTTA GCATTTTCAA CACCCCCAGA
    AAGCTGGGCA ACAGCCTCCT GAAGAGAGGC CTCAACAAGA AAACGACTCC CAAAAATAGC
    CCCCGGGGGC GGGGGGCAAG TGGCAGCGCC GGCAGCACTT CAGGCAAATC CCCGCACCGC
    TCTCTCCGGA AGTCACCCAG CAGGCGGTCT CCGAGGGCCT CCACCGCAAA GTCCCCCAAA
    GCTTCAACCA AGGTGGGGCA GGAGCTGCAG ATACAGTTTT TCGAGCGCAA GCATCAGAGG
    AACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004920192.1
    CDS594..7502
    Translation

    Target ORF information:

    RefSeq Version XM_004920135.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004920135.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CGGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTAT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGGGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAGGAAAC AACTGGAACG AAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGCAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGCAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAG TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGGGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCA
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAA
    GAGCTGGAGG CGGCAAAGAA AGAGCAAAGT GAGCAGGTGC AGGAGCTGAA CAAGCAGCTG
    AGCCAGCAGG AGAAGGCCTT ACGGAGCCAG CAGCACAAGC TGAAGCAGCA GGAAGGTGAG
    ACTCATGAGG AGGCTGAGAA GGCTCAGAAG CGAGTGGTGG AGTTGGAGTC CCAGGTGGAA
    CAGCAGACTC AGGCCGTGGA ACATTACAAA GCTCAGATGG AGAAAGCCAA GCTGTATTAC
    GATGCCAAGA AGAAGCAGAA CCAGGAGCTA AGCGAGGAGC TGCAAAGCCA CATTAGAGAG
    CAGGAACATC TGCGCAAGGA GAACGTAGAT CTAAAGGCAG AGTCTGAGCA GCTCAGTAAG
    GAGCTACAGC ATTCGCTGCT ACAGAACAAA GAGGCAGAGC AAAACTGCAA GAACCTGTCC
    AATCGGGTTA GGAGTCTGGA GGCCCAGGTG GAGTATGCCG ACCGGCAGCT GCGAGACCTG
    GGGAAGTTCC AGTTGGCCAC GGATTCCATG AAGAGTCGGG AAACTTTCGG AGCCCCCCGT
    GTAACACGGA GCCATGCAGA TGTCAGTATA GACAGCCTGG ACTTGAGCTT TGAGGATGAC
    CAGCTTTTGA ACTCAACAAG TAAGAATGGC CGCTGCAACG AGGAACCTGC CACATCGTCA
    GTACATGCCA GCAGCATGGA GTCTCCTACC ACAGGCCAAC TGCCCAAAAA GGTGGAGTCT
    CTGGAAAGCC TCTACTTCAC ACCTATTCCA ACCCGGGCAC AGTCCAAACT GGAAAGTAGC
    CTTGGTTCCA TCGGGGACCT GTCCCTAGAC TCCAGCAAGA AAACGCGGTC TGCAAGGAGA
    CGCACCACCC AGATCATTAA CATCACAATG ACCAAGAAGA CGAAGGAGGA ACCGGAAGCA
    GAGAGTGCCA ACACGTCATT CTACAGCCTG CGCTCCGCTC CGTCCTATCA GAGTCTCCAC
    CAGCAGAAGC CCCGGAGGCC TGGGCGTCCT CAGGCAGCGG TTTCTGCCCC AGCCCTGGCC
    AGCCTCCCAT CCCAGGAATC CCTGGCCAAG ACAGAACAGT TCTCTGATGA CAGCTTTAAT
    AACTCCGTAT TGATGAATTT ACCTGGTTAC CGACCCCCCG CGCGTAGATC TGCAAGGCTT
    TCACAAACTG GAGGCCGAAG CAGTTTCTAC ATGAGCACGT GCCAGGATGA GCCAGACCCA
    CAGGAAGACT GGAACCGGAT CGCTGAGTTG CAGGCCCGGA ACAAGGCCTG CCCACCCCAC
    CTCAAGACTT CCTACCCACT GGAGTCCAGG CCGTCACTCT TAAGCTCCAC CATTACCGAT
    GAAGAGGTCA AGTTGGGAGA CCCTAAAGAG ACGCTCAGGA GAGCCACCAT GCTGCCCTCC
    CAGATACAAG AGTCCACAGG CAGTACCCGC AGAATGAGCT TGGCTGCCTC TGAACATATG
    AGGGGACCCA GCATCAGCAC CAGGCAGCAG ATGAAGCGCG TGTCCGAGGA GTCTCACCTT
    GGCCCAGACA CCCCAGAAGC CAAGAAGTCT GCCACTTGCT TTCCTCGGCC CATGACCCCA
    AAGGATAAAC ACGACGCACG CAGATTGTCC ACTGTGGAAA GCAAAGGAAA CTCCAGCCAT
    CAGCAGGCGC AGCCCACTCG CCGTCAGTCT ACAGCATTTA GCATTTTCAA CACCCCCAGA
    AAGCTGGGCA ACAGCCTCCT GAAGAGAGGC CTCAACAAGA AAACGACTCC CAAAAATAGC
    CCCCGGGGGC GGGGGGCAAG TGGCAGCGCC GGCAGCACTT CAGGCAAATC CCCGCACCGC
    TCTCTCCGGA AGTCACCCAG CAGGCGGTCT CCGAGGGCCT CCACCGCAAA GTCCCCCAAA
    GCTTCAACCA AGGTGGGGCA GGAGCTGCAG ATACAGTTTT TCGAGCGCAA GCATCAGAGG
    AACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa19634
    Clone ID Related Accession (Same CDS sequence) XM_004920136.3
    Accession Version XM_004920136.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nuclear mitotic apparatus protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683952.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004920136.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CGGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTAT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGGGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAGGAAAC AACTGGAACG AAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGCAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGCAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAG TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGGGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCA
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAA
    GAGCTGGAGG CGGCAAAGAA AGAGCAAAGT GAGCAGGTGC AGGAGCTGAA CAAGCAGCTG
    AGCCAGCAGG AGAAGGCCTT ACGGAGCCAG CAGCACAAGC TGAAGCAGCA GGAAGGTGAG
    ACTCATGAGG AGGCTGAGAA GGCTCAGAAG CGAGTGGTGG AGTTGGAGTC CCAGGTGGAA
    CAGCAGACTC AGGCCGTGGA ACATTACAAA GCTCAGATGG AGAAAGCCAA GCTGTATTAC
    GATGCCAAGA AGAAGCAGAA CCAGGAGCTA AGCGAGGAGC TGCAAAGCCA CATTAGAGAG
    CAGGAACATC TGCGCAAGGA GAACGTAGAT CTAAAGGCAG AGTCTGAGCA GCTCAGTAAG
    GAGCTACAGC ATTCGCTGCT ACAGAACAAA GAGGCAGAGC AAAACTGCAA GAACCTGTCC
    AATCGGGTTA GGAGTCTGGA GGCCCAGGTG GAGTATGCCG ACCGGCAGCT GCGAGACCTG
    GGGAAGTTCC AGTTGGCCAC GGATTCCATG AAGAGTCGGG AAACTTTCGG AGCCCCCCGT
    GTAACACGGA GCCATGCAGA TGTCAGTATA GACAGCCTGG ACTTGAGCTT TGAGGATGAC
    CAGCTTTTGA ACTCAACAAG TAAGAATGGC CGCTGCAACG AGGAACCTGC CACATCGTCA
    GTACATGCCA GCAGCATGGA GTCTCCTACC ACAGGCCAAC TGCCCAAAAA GGTGGAGTCT
    CTGGAAAGCC TCTACTTCAC ACCTATTCCA ACCCGGGCAC AGTCCAAACT GGAAAGTAGC
    CTTGGTTCCA TCGGGGACCT GTCCCTAGAC TCCAGCAAGA AAACGCGGTC TGCAAGGAGA
    CGCACCACCC AGATCATTAA CATCACAATG ACCAAGAAGA CGAAGGAGGA ACCGGAAGCA
    GAGAGTGCCA ACACGTCATT CTACAGCCTG CGCTCCGCTC CGTCCTATCA GAGTCTCCAC
    CAGCAGAAGC CCCGGAGGCC TGGGCGTCCT CAGGCAGCGG TTTCTGCCCC AGCCCTGGCC
    AGCCTCCCAT CCCAGGAATC CCTGGCCAAG ACAGAACAGT TCTCTGATGA CAGCTTTAAT
    AACTCCGTAT TGATGAATTT ACCTGGTTAC CGACCCCCCG CGCGTAGATC TGCAAGGCTT
    TCACAAACTG GAGGCCGAAG CAGTTTCTAC ATGAGCACGT GCCAGGATGA GCCAGACCCA
    CAGGAAGACT GGAACCGGAT CGCTGAGTTG CAGGCCCGGA ACAAGGCCTG CCCACCCCAC
    CTCAAGACTT CCTACCCACT GGAGTCCAGG CCGTCACTCT TAAGCTCCAC CATTACCGAT
    GAAGAGGTCA AGTTGGGAGA CCCTAAAGAG ACGCTCAGGA GAGCCACCAT GCTGCCCTCC
    CAGATACAAG AGTCCACAGG CAGTACCCGC AGAATGAGCT TGGCTGCCTC TGAACATATG
    AGGGGACCCA GCATCAGCAC CAGGCAGCAG ATGAAGCGCG TGTCCGAGGA GTCTCACCTT
    GGCCCAGACA CCCCAGAAGC CAAGAAGTCT GCCACTTGCT TTCCTCGGCC CATGACCCCA
    AAGGATAAAC ACGACGCACG CAGATTGTCC ACTGTGGAAA GCAAAGGAAA CTCCAGCCAT
    CAGCAGGCGC AGCCCACTCG CCGTCAGTCT ACAGCATTTA GCATTTTCAA CACCCCCAGA
    AAGCTGGGCA ACAGCCTCCT GAAGAGAGGC CTCAACAAGA AAACGACTCC CAAAAATAGC
    CCCCGGGGGC GGGGGGCAAG TGGCAGCGCC GGCAGCACTT CAGGCAAATC CCCGCACCGC
    TCTCTCCGGA AGTCACCCAG CAGGCGGTCT CCGAGGGCCT CCACCGCAAA GTCCCCCAAA
    GCTTCAACCA AGTTTTTCGA GCGCAAGCAT CAGAGGAACA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004920193.1
    CDS594..7478
    Translation

    Target ORF information:

    RefSeq Version XM_004920136.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004920136.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CGGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTAT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGGGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAGGAAAC AACTGGAACG AAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGCAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGCAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAG TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGGGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCA
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAA
    GAGCTGGAGG CGGCAAAGAA AGAGCAAAGT GAGCAGGTGC AGGAGCTGAA CAAGCAGCTG
    AGCCAGCAGG AGAAGGCCTT ACGGAGCCAG CAGCACAAGC TGAAGCAGCA GGAAGGTGAG
    ACTCATGAGG AGGCTGAGAA GGCTCAGAAG CGAGTGGTGG AGTTGGAGTC CCAGGTGGAA
    CAGCAGACTC AGGCCGTGGA ACATTACAAA GCTCAGATGG AGAAAGCCAA GCTGTATTAC
    GATGCCAAGA AGAAGCAGAA CCAGGAGCTA AGCGAGGAGC TGCAAAGCCA CATTAGAGAG
    CAGGAACATC TGCGCAAGGA GAACGTAGAT CTAAAGGCAG AGTCTGAGCA GCTCAGTAAG
    GAGCTACAGC ATTCGCTGCT ACAGAACAAA GAGGCAGAGC AAAACTGCAA GAACCTGTCC
    AATCGGGTTA GGAGTCTGGA GGCCCAGGTG GAGTATGCCG ACCGGCAGCT GCGAGACCTG
    GGGAAGTTCC AGTTGGCCAC GGATTCCATG AAGAGTCGGG AAACTTTCGG AGCCCCCCGT
    GTAACACGGA GCCATGCAGA TGTCAGTATA GACAGCCTGG ACTTGAGCTT TGAGGATGAC
    CAGCTTTTGA ACTCAACAAG TAAGAATGGC CGCTGCAACG AGGAACCTGC CACATCGTCA
    GTACATGCCA GCAGCATGGA GTCTCCTACC ACAGGCCAAC TGCCCAAAAA GGTGGAGTCT
    CTGGAAAGCC TCTACTTCAC ACCTATTCCA ACCCGGGCAC AGTCCAAACT GGAAAGTAGC
    CTTGGTTCCA TCGGGGACCT GTCCCTAGAC TCCAGCAAGA AAACGCGGTC TGCAAGGAGA
    CGCACCACCC AGATCATTAA CATCACAATG ACCAAGAAGA CGAAGGAGGA ACCGGAAGCA
    GAGAGTGCCA ACACGTCATT CTACAGCCTG CGCTCCGCTC CGTCCTATCA GAGTCTCCAC
    CAGCAGAAGC CCCGGAGGCC TGGGCGTCCT CAGGCAGCGG TTTCTGCCCC AGCCCTGGCC
    AGCCTCCCAT CCCAGGAATC CCTGGCCAAG ACAGAACAGT TCTCTGATGA CAGCTTTAAT
    AACTCCGTAT TGATGAATTT ACCTGGTTAC CGACCCCCCG CGCGTAGATC TGCAAGGCTT
    TCACAAACTG GAGGCCGAAG CAGTTTCTAC ATGAGCACGT GCCAGGATGA GCCAGACCCA
    CAGGAAGACT GGAACCGGAT CGCTGAGTTG CAGGCCCGGA ACAAGGCCTG CCCACCCCAC
    CTCAAGACTT CCTACCCACT GGAGTCCAGG CCGTCACTCT TAAGCTCCAC CATTACCGAT
    GAAGAGGTCA AGTTGGGAGA CCCTAAAGAG ACGCTCAGGA GAGCCACCAT GCTGCCCTCC
    CAGATACAAG AGTCCACAGG CAGTACCCGC AGAATGAGCT TGGCTGCCTC TGAACATATG
    AGGGGACCCA GCATCAGCAC CAGGCAGCAG ATGAAGCGCG TGTCCGAGGA GTCTCACCTT
    GGCCCAGACA CCCCAGAAGC CAAGAAGTCT GCCACTTGCT TTCCTCGGCC CATGACCCCA
    AAGGATAAAC ACGACGCACG CAGATTGTCC ACTGTGGAAA GCAAAGGAAA CTCCAGCCAT
    CAGCAGGCGC AGCCCACTCG CCGTCAGTCT ACAGCATTTA GCATTTTCAA CACCCCCAGA
    AAGCTGGGCA ACAGCCTCCT GAAGAGAGGC CTCAACAAGA AAACGACTCC CAAAAATAGC
    CCCCGGGGGC GGGGGGCAAG TGGCAGCGCC GGCAGCACTT CAGGCAAATC CCCGCACCGC
    TCTCTCCGGA AGTCACCCAG CAGGCGGTCT CCGAGGGCCT CCACCGCAAA GTCCCCCAAA
    GCTTCAACCA AGTTTTTCGA GCGCAAGCAT CAGAGGAACA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31960
    Clone ID Related Accession (Same CDS sequence) XM_012954898.2
    Accession Version XM_012954898.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nuclear mitotic apparatus protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683952.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954898.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CGGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTAT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGGGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAGGAAAC AACTGGAACG AAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGCAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGCAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAG TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGGGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCA
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAA
    GAGCTGGAGG CGGCAAAGAA AGAGCAAAGT GAGCAGGTGC AGGAGCTGAA CAAGCAGCTG
    AGCCAGCAGG AGAAGGCCTT ACGGAGCCAG CAGCACAAGC TGAAGCAGCA GGAAGGTGAG
    ACTCATGAGG AGGCTGAGAA GGCTCAGAAG CGAGTGGTGG AGTTGGAGTC CCAGGTGGAA
    CAGCAGACTC AGGCCGTGGA ACATTACAAA GCTCAGATGG AGAAAGCCAA GCTGTATTAC
    GATGCCAAGA AGAAGCAGAA CCAGGAGCTA AGCGAGGAGC TGCAAAGCCA CATTAGAGAG
    CAGGAACATC TGCGCAAGGA GAACGTAGAT CTAAAGGCAG AGTCTGAGCA GCTCAGTAAG
    GAGCTACAGC ATTCGCTGCT ACAGAACAAA GAGGCAGAGC AAAACTGCAA GAACCTGTCC
    AATCGGGTTA GGAGTCTGGA GGCCCAGGTG GAGTATGCCG ACCGGCAGCT GCGAGACCTG
    GGGAAGTTCC AGTTGGCCAC GGATTCCATG AAGAGTCGGG AAACTTTCGG AGCCCCCCGT
    GTAACACGGA GCCATGCAGA TGTCAGTATA GACAGCCTGG ACTTGAGCTT TGAGGATGAC
    CAGCTTTTGA ACTCAACAAG TAAGAATGGC CGCTGCAACG AGGAACCTGC CACATCGTCA
    GTACATGCCA GCAGCATGGA GTCTCCTACC ACAGGCCAAC TGCCCAAAAA GGTGGAGTCT
    CTGGAAAGCC TCTACTTCAC ACCTATTCCA ACCCGGGCAC AGTCCAAACT GGAAAGTAGC
    CTTGGTTCCA TCGGGGACCT GTCCCTAGAC TCCAGCAAGA AAACGCGGTC TGCAAGGAGA
    CGCACCACCC AGATCATTAA CATCACAATG ACCAAGAAGA CGAAGGAGGA ACCGGAAGCA
    GAGAGTGCCA ACACGTCATT CTACAGCCTG CGCTCCGCTC CGTCCTATCA GAGTCTCCAC
    CAGCAGAAGC CCCGGAGGCC TGGGCGTCCT CAGGCAGCGG TTTCTGCCCC AGCCCTGGCC
    AGCCTCCCAT CCCAGGAATC CCTGGCCAAG ACAGAACAGT TCTCTGATGA CAGCTTTAAT
    AACTCCGTAT TGATGAATTT ACCTGGTTAC CGACCCCCCG CGCGTAGATC TGCAAGGCTT
    TCACAAACTG GAGGCCGAAG CAGTTTCTAC ATGAGCACGT GCCAGGATGA GCCAGACCCA
    CAGGAAGACT GGAACCGGAT CGCTGAGTTG CAGGCCCGGA ACAAGGCCTG CCCACCCCAC
    CTCAAGACTT CCTACCCACT GGAGTCCAGG CCGTCACTCT TAAGCTCCAC CATTACCGAT
    GAAGAGGTCA AGTTGGGAGA CCCTAAAGAG ACGCTCAGGA GAGCCACCAT GCTGCCCTCC
    CAGATACAAG AGTCCACAGG CAGTACCCGC AGAATGAGCT TGGCTGCCTC TGAACATATG
    AGGGGACCCA GCATCAGCAC CAGGCAGCAG ATGAAGCGCG TGTCCGAGGA GTCTCACCTT
    GGCCCAGACA CCCCAGAAGC CAAGAAGTCT GCCACTTGCT TTCCTCGGCC CATGACCCCA
    AAGGATAAAC ACGACGCACG CAGATTGTCC ACTGTGGAAA GCAAAGGAAA CTCCAGCCAT
    CAGGCGCAGC CCACTCGCCG TCAGTCTACA GCATTTAGCA TTTTCAACAC CCCCAGAAAG
    CTGGGCAACA GCCTCCTGAA GAGAGGCCTC AACAAGAAAA CGACTCCCAA AAATAGCCCC
    CGGGGGCGGG GGGCAAGTGG CAGCGCCGGC AGCACTTCAG GCAAATCCCC GCACCGCTCT
    CTCCGGAAGT CACCCAGCAG GCGGTCTCCG AGGGCCTCCA CCGCAAAGTC CCCCAAAGCT
    TCAACCAAGG TGGGGCAGGA GCTGCAGATA CAGTTTTTCG AGCGCAAGCA TCAGAGGAAC
    AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012810352.1
    CDS595..7500
    Translation

    Target ORF information:

    RefSeq Version XM_012954898.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012954898.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CGGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTAT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGGGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAGGAAAC AACTGGAACG AAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGCAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGCAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAG TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGGGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCA
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAA
    GAGCTGGAGG CGGCAAAGAA AGAGCAAAGT GAGCAGGTGC AGGAGCTGAA CAAGCAGCTG
    AGCCAGCAGG AGAAGGCCTT ACGGAGCCAG CAGCACAAGC TGAAGCAGCA GGAAGGTGAG
    ACTCATGAGG AGGCTGAGAA GGCTCAGAAG CGAGTGGTGG AGTTGGAGTC CCAGGTGGAA
    CAGCAGACTC AGGCCGTGGA ACATTACAAA GCTCAGATGG AGAAAGCCAA GCTGTATTAC
    GATGCCAAGA AGAAGCAGAA CCAGGAGCTA AGCGAGGAGC TGCAAAGCCA CATTAGAGAG
    CAGGAACATC TGCGCAAGGA GAACGTAGAT CTAAAGGCAG AGTCTGAGCA GCTCAGTAAG
    GAGCTACAGC ATTCGCTGCT ACAGAACAAA GAGGCAGAGC AAAACTGCAA GAACCTGTCC
    AATCGGGTTA GGAGTCTGGA GGCCCAGGTG GAGTATGCCG ACCGGCAGCT GCGAGACCTG
    GGGAAGTTCC AGTTGGCCAC GGATTCCATG AAGAGTCGGG AAACTTTCGG AGCCCCCCGT
    GTAACACGGA GCCATGCAGA TGTCAGTATA GACAGCCTGG ACTTGAGCTT TGAGGATGAC
    CAGCTTTTGA ACTCAACAAG TAAGAATGGC CGCTGCAACG AGGAACCTGC CACATCGTCA
    GTACATGCCA GCAGCATGGA GTCTCCTACC ACAGGCCAAC TGCCCAAAAA GGTGGAGTCT
    CTGGAAAGCC TCTACTTCAC ACCTATTCCA ACCCGGGCAC AGTCCAAACT GGAAAGTAGC
    CTTGGTTCCA TCGGGGACCT GTCCCTAGAC TCCAGCAAGA AAACGCGGTC TGCAAGGAGA
    CGCACCACCC AGATCATTAA CATCACAATG ACCAAGAAGA CGAAGGAGGA ACCGGAAGCA
    GAGAGTGCCA ACACGTCATT CTACAGCCTG CGCTCCGCTC CGTCCTATCA GAGTCTCCAC
    CAGCAGAAGC CCCGGAGGCC TGGGCGTCCT CAGGCAGCGG TTTCTGCCCC AGCCCTGGCC
    AGCCTCCCAT CCCAGGAATC CCTGGCCAAG ACAGAACAGT TCTCTGATGA CAGCTTTAAT
    AACTCCGTAT TGATGAATTT ACCTGGTTAC CGACCCCCCG CGCGTAGATC TGCAAGGCTT
    TCACAAACTG GAGGCCGAAG CAGTTTCTAC ATGAGCACGT GCCAGGATGA GCCAGACCCA
    CAGGAAGACT GGAACCGGAT CGCTGAGTTG CAGGCCCGGA ACAAGGCCTG CCCACCCCAC
    CTCAAGACTT CCTACCCACT GGAGTCCAGG CCGTCACTCT TAAGCTCCAC CATTACCGAT
    GAAGAGGTCA AGTTGGGAGA CCCTAAAGAG ACGCTCAGGA GAGCCACCAT GCTGCCCTCC
    CAGATACAAG AGTCCACAGG CAGTACCCGC AGAATGAGCT TGGCTGCCTC TGAACATATG
    AGGGGACCCA GCATCAGCAC CAGGCAGCAG ATGAAGCGCG TGTCCGAGGA GTCTCACCTT
    GGCCCAGACA CCCCAGAAGC CAAGAAGTCT GCCACTTGCT TTCCTCGGCC CATGACCCCA
    AAGGATAAAC ACGACGCACG CAGATTGTCC ACTGTGGAAA GCAAAGGAAA CTCCAGCCAT
    CAGGCGCAGC CCACTCGCCG TCAGTCTACA GCATTTAGCA TTTTCAACAC CCCCAGAAAG
    CTGGGCAACA GCCTCCTGAA GAGAGGCCTC AACAAGAAAA CGACTCCCAA AAATAGCCCC
    CGGGGGCGGG GGGCAAGTGG CAGCGCCGGC AGCACTTCAG GCAAATCCCC GCACCGCTCT
    CTCCGGAAGT CACCCAGCAG GCGGTCTCCG AGGGCCTCCA CCGCAAAGTC CCCCAAAGCT
    TCAACCAAGG TGGGGCAGGA GCTGCAGATA CAGTTTTTCG AGCGCAAGCA TCAGAGGAAC
    AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31961
    Clone ID Related Accession (Same CDS sequence) XM_012954899.2
    Accession Version XM_012954899.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nuclear mitotic apparatus protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683952.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954899.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
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    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
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    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CGGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTAT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGGGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAGGAAAC AACTGGAACG AAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGCAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGCAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAG TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGGGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCA
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAG
    GAGCTGAACA AGCAGCTGAG CCAGCAGGAG AAGGCCTTAC GGAGCCAGCA GCACAAGCTG
    AAGCAGCAGG AAGGTGAGAC TCATGAGGAG GCTGAGAAGG CTCAGAAGCG AGTGGTGGAG
    TTGGAGTCCC AGGTGGAACA GCAGACTCAG GCCGTGGAAC ATTACAAAGC TCAGATGGAG
    AAAGCCAAGC TGTATTACGA TGCCAAGAAG AAGCAGAACC AGGAGCTAAG CGAGGAGCTG
    CAAAGCCACA TTAGAGAGCA GGAACATCTG CGCAAGGAGA ACGTAGATCT AAAGGCAGAG
    TCTGAGCAGC TCAGTAAGGA GCTACAGCAT TCGCTGCTAC AGAACAAAGA GGCAGAGCAA
    AACTGCAAGA ACCTGTCCAA TCGGGTTAGG AGTCTGGAGG CCCAGGTGGA GTATGCCGAC
    CGGCAGCTGC GAGACCTGGG GAAGTTCCAG TTGGCCACGG ATTCCATGAA GAGTCGGGAA
    ACTTTCGGAG CCCCCCGTGT AACACGGAGC CATGCAGATG TCAGTATAGA CAGCCTGGAC
    TTGAGCTTTG AGGATGACCA GCTTTTGAAC TCAACAAGTA AGAATGGCCG CTGCAACGAG
    GAACCTGCCA CATCGTCAGT ACATGCCAGC AGCATGGAGT CTCCTACCAC AGGCCAACTG
    CCCAAAAAGG TGGAGTCTCT GGAAAGCCTC TACTTCACAC CTATTCCAAC CCGGGCACAG
    TCCAAACTGG AAAGTAGCCT TGGTTCCATC GGGGACCTGT CCCTAGACTC CAGCAAGAAA
    ACGCGGTCTG CAAGGAGACG CACCACCCAG ATCATTAACA TCACAATGAC CAAGAAGACG
    AAGGAGGAAC CGGAAGCAGA GAGTGCCAAC ACGTCATTCT ACAGCCTGCG CTCCGCTCCG
    TCCTATCAGA GTCTCCACCA GCAGAAGCCC CGGAGGCCTG GGCGTCCTCA GGCAGCGGTT
    TCTGCCCCAG CCCTGGCCAG CCTCCCATCC CAGGAATCCC TGGCCAAGAC AGAACAGTTC
    TCTGATGACA GCTTTAATAA CTCCGTATTG ATGAATTTAC CTGGTTACCG ACCCCCCGCG
    CGTAGATCTG CAAGGCTTTC ACAAACTGGA GGCCGAAGCA GTTTCTACAT GAGCACGTGC
    CAGGATGAGC CAGACCCACA GGAAGACTGG AACCGGATCG CTGAGTTGCA GGCCCGGAAC
    AAGGCCTGCC CACCCCACCT CAAGACTTCC TACCCACTGG AGTCCAGGCC GTCACTCTTA
    AGCTCCACCA TTACCGATGA AGAGGTCAAG TTGGGAGACC CTAAAGAGAC GCTCAGGAGA
    GCCACCATGC TGCCCTCCCA GATACAAGAG TCCACAGGCA GTACCCGCAG AATGAGCTTG
    GCTGCCTCTG AACATATGAG GGGACCCAGC ATCAGCACCA GGCAGCAGAT GAAGCGCGTG
    TCCGAGGAGT CTCACCTTGG CCCAGACACC CCAGAAGCCA AGAAGTCTGC CACTTGCTTT
    CCTCGGCCCA TGACCCCAAA GGATAAACAC GACGCACGCA GATTGTCCAC TGTGGAAAGC
    AAAGGAAACT CCAGCCATCA GCAGGCGCAG CCCACTCGCC GTCAGTCTAC AGCATTTAGC
    ATTTTCAACA CCCCCAGAAA GCTGGGCAAC AGCCTCCTGA AGAGAGGCCT CAACAAGAAA
    ACGACTCCCA AAAATAGCCC CCGGGGGCGG GGGGCAAGTG GCAGCGCCGG CAGCACTTCA
    GGCAAATCCC CGCACCGCTC TCTCCGGAAG TCACCCAGCA GGCGGTCTCC GAGGGCCTCC
    ACCGCAAAGT CCCCCAAAGC TTCAACCAAG GTGGGGCAGG AGCTGCAGAT ACAGTTTTTC
    GAGCGCAAGC ATCAGAGGAA CAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012810353.1
    CDS594..7460
    Translation

    Target ORF information:

    RefSeq Version XM_012954899.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012954899.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CGGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTAT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGGGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAGGAAAC AACTGGAACG AAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGCAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGCAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAG TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGGGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCA
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAG
    GAGCTGAACA AGCAGCTGAG CCAGCAGGAG AAGGCCTTAC GGAGCCAGCA GCACAAGCTG
    AAGCAGCAGG AAGGTGAGAC TCATGAGGAG GCTGAGAAGG CTCAGAAGCG AGTGGTGGAG
    TTGGAGTCCC AGGTGGAACA GCAGACTCAG GCCGTGGAAC ATTACAAAGC TCAGATGGAG
    AAAGCCAAGC TGTATTACGA TGCCAAGAAG AAGCAGAACC AGGAGCTAAG CGAGGAGCTG
    CAAAGCCACA TTAGAGAGCA GGAACATCTG CGCAAGGAGA ACGTAGATCT AAAGGCAGAG
    TCTGAGCAGC TCAGTAAGGA GCTACAGCAT TCGCTGCTAC AGAACAAAGA GGCAGAGCAA
    AACTGCAAGA ACCTGTCCAA TCGGGTTAGG AGTCTGGAGG CCCAGGTGGA GTATGCCGAC
    CGGCAGCTGC GAGACCTGGG GAAGTTCCAG TTGGCCACGG ATTCCATGAA GAGTCGGGAA
    ACTTTCGGAG CCCCCCGTGT AACACGGAGC CATGCAGATG TCAGTATAGA CAGCCTGGAC
    TTGAGCTTTG AGGATGACCA GCTTTTGAAC TCAACAAGTA AGAATGGCCG CTGCAACGAG
    GAACCTGCCA CATCGTCAGT ACATGCCAGC AGCATGGAGT CTCCTACCAC AGGCCAACTG
    CCCAAAAAGG TGGAGTCTCT GGAAAGCCTC TACTTCACAC CTATTCCAAC CCGGGCACAG
    TCCAAACTGG AAAGTAGCCT TGGTTCCATC GGGGACCTGT CCCTAGACTC CAGCAAGAAA
    ACGCGGTCTG CAAGGAGACG CACCACCCAG ATCATTAACA TCACAATGAC CAAGAAGACG
    AAGGAGGAAC CGGAAGCAGA GAGTGCCAAC ACGTCATTCT ACAGCCTGCG CTCCGCTCCG
    TCCTATCAGA GTCTCCACCA GCAGAAGCCC CGGAGGCCTG GGCGTCCTCA GGCAGCGGTT
    TCTGCCCCAG CCCTGGCCAG CCTCCCATCC CAGGAATCCC TGGCCAAGAC AGAACAGTTC
    TCTGATGACA GCTTTAATAA CTCCGTATTG ATGAATTTAC CTGGTTACCG ACCCCCCGCG
    CGTAGATCTG CAAGGCTTTC ACAAACTGGA GGCCGAAGCA GTTTCTACAT GAGCACGTGC
    CAGGATGAGC CAGACCCACA GGAAGACTGG AACCGGATCG CTGAGTTGCA GGCCCGGAAC
    AAGGCCTGCC CACCCCACCT CAAGACTTCC TACCCACTGG AGTCCAGGCC GTCACTCTTA
    AGCTCCACCA TTACCGATGA AGAGGTCAAG TTGGGAGACC CTAAAGAGAC GCTCAGGAGA
    GCCACCATGC TGCCCTCCCA GATACAAGAG TCCACAGGCA GTACCCGCAG AATGAGCTTG
    GCTGCCTCTG AACATATGAG GGGACCCAGC ATCAGCACCA GGCAGCAGAT GAAGCGCGTG
    TCCGAGGAGT CTCACCTTGG CCCAGACACC CCAGAAGCCA AGAAGTCTGC CACTTGCTTT
    CCTCGGCCCA TGACCCCAAA GGATAAACAC GACGCACGCA GATTGTCCAC TGTGGAAAGC
    AAAGGAAACT CCAGCCATCA GCAGGCGCAG CCCACTCGCC GTCAGTCTAC AGCATTTAGC
    ATTTTCAACA CCCCCAGAAA GCTGGGCAAC AGCCTCCTGA AGAGAGGCCT CAACAAGAAA
    ACGACTCCCA AAAATAGCCC CCGGGGGCGG GGGGCAAGTG GCAGCGCCGG CAGCACTTCA
    GGCAAATCCC CGCACCGCTC TCTCCGGAAG TCACCCAGCA GGCGGTCTCC GAGGGCCTCC
    ACCGCAAAGT CCCCCAAAGC TTCAACCAAG GTGGGGCAGG AGCTGCAGAT ACAGTTTTTC
    GAGCGCAAGC ATCAGAGGAA CAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31962
    Clone ID Related Accession (Same CDS sequence) XM_012954900.2
    Accession Version XM_012954900.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nuclear mitotic apparatus protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683952.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954900.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CGGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTAT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGGGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAGGAAAC AACTGGAACG AAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGCAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGCAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAG TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGGGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCA
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAG
    GAGCTGAACA AGCAGCTGAG CCAGCAGGAG AAGGCCTTAC GGAGCCAGCA GCACAAGCTG
    AAGCAGCAGG AAGGTGAGAC TCATGAGGAG GCTGAGAAGG CTCAGAAGCG AGTGGTGGAG
    TTGGAGTCCC AGGTGGAACA GCAGACTCAG GCCGTGGAAC ATTACAAAGC TCAGATGGAG
    AAAGCCAAGC TGTATTACGA TGCCAAGAAG AAGCAGAACC AGGAGCTAAG CGAGGAGCTG
    CAAAGCCACA TTAGAGAGCA GGAACATCTG CGCAAGGAGA ACGTAGATCT AAAGGCAGAG
    TCTGAGCAGC TCAGTAAGGA GCTACAGCAT TCGCTGCTAC AGAACAAAGA GGCAGAGCAA
    AACTGCAAGA ACCTGTCCAA TCGGGTTAGG AGTCTGGAGG CCCAGGTGGA GTATGCCGAC
    CGGCAGCTGC GAGACCTGGG GAAGTTCCAG TTGGCCACGG ATTCCATGAA GAGTCGGGAA
    ACTTTCGGAG CCCCCCGTGT AACACGGAGC CATGCAGATG TCAGTATAGA CAGCCTGGAC
    TTGAGCTTTG AGGATGACCA GCTTTTGAAC TCAACAAGTA AGAATGGCCG CTGCAACGAG
    GAACCTGCCA CATCGTCAGT ACATGCCAGC AGCATGGAGT CTCCTACCAC AGGCCAACTG
    CCCAAAAAGG TGGAGTCTCT GGAAAGCCTC TACTTCACAC CTATTCCAAC CCGGGCACAG
    TCCAAACTGG AAAGTAGCCT TGGTTCCATC GGGGACCTGT CCCTAGACTC CAGCAAGAAA
    ACGCGGTCTG CAAGGAGACG CACCACCCAG ATCATTAACA TCACAATGAC CAAGAAGACG
    AAGGAGGAAC CGGAAGCAGA GAGTGCCAAC ACGTCATTCT ACAGCCTGCG CTCCGCTCCG
    TCCTATCAGA GTCTCCACCA GCAGAAGCCC CGGAGGCCTG GGCGTCCTCA GGCAGCGGTT
    TCTGCCCCAG CCCTGGCCAG CCTCCCATCC CAGGAATCCC TGGCCAAGAC AGAACAGTTC
    TCTGATGACA GCTTTAATAA CTCCGTATTG ATGAATTTAC CTGGTTACCG ACCCCCCGCG
    CGTAGATCTG CAAGGCTTTC ACAAACTGGA GGCCGAAGCA GTTTCTACAT GAGCACGTGC
    CAGGATGAGC CAGACCCACA GGAAGACTGG AACCGGATCG CTGAGTTGCA GGCCCGGAAC
    AAGGCCTGCC CACCCCACCT CAAGACTTCC TACCCACTGG AGTCCAGGCC GTCACTCTTA
    AGCTCCACCA TTACCGATGA AGAGGTCAAG TTGGGAGACC CTAAAGAGAC GCTCAGGAGA
    GCCACCATGC TGCCCTCCCA GATACAAGAG TCCACAGGCA GTACCCGCAG AATGAGCTTG
    GCTGCCTCTG AACATATGAG GGGACCCAGC ATCAGCACCA GGCAGCAGAT GAAGCGCGTG
    TCCGAGGAGT CTCACCTTGG CCCAGACACC CCAGAAGCCA AGAAGTCTGC CACTTGCTTT
    CCTCGGCCCA TGACCCCAAA GGATAAACAC GACGCACGCA GATTGTCCAC TGTGGAAAGC
    AAAGGAAACT CCAGCCATCA GCAGGCGCAG CCCACTCGCC GTCAGTCTAC AGCATTTAGC
    ATTTTCAACA CCCCCAGAAA GCTGGGCAAC AGCCTCCTGA AGAGAGGCCT CAACAAGAAA
    ACGACTCCCA AAAATAGCCC CCGGGGGCGG GGGGCAAGTG GCAGCGCCGG CAGCACTTCA
    GGCAAATCCC CGCACCGCTC TCTCCGGAAG TCACCCAGCA GGCGGTCTCC GAGGGCCTCC
    ACCGCAAAGT CCCCCAAAGC TTCAACCAAG TTTTTCGAGC GCAAGCATCA GAGGAACAAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012810354.1
    CDS594..7436
    Translation

    Target ORF information:

    RefSeq Version XM_012954900.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nuclear mitotic apparatus protein 1 (numa1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012954900.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCGGCGC ACAGCGGCAA GATGGAAGCA TTGCTCTGCT GGGTGAACAG CCTGAAGGTG 
    GGGGAACCAA TCGAGAGATT TTCACAGCTG CAGGATCTGG GCATTCTGCT GAAAGTTGTC
    GGCATATTAT CGGGGAACTC CGAGGAAACG GCCCTTGTCC TACAGCAGAT CCCGGAGGAG
    CGGCTGAACT TCCTCTGTGC CTTCTTACAA AGGTATTGCC GGCCGGGCTC CTCAGCTGAA
    AACTTGGTGC AATGGCAGAA GATTCTGCAG GGGGAGAACC TGGAAGTGGA ACTTTCCAAG
    GCAATCGTCC TTCTCTTCTA TTACTCAACT ATGAGCTCCA AAAACCCGAA GAAATCTGAG
    GATTTTGATC ATAAAACTCA GACAGAGCTG GCCTCCATCT TGCGCTTTGT ACTGGATAAT
    GAAGATGCTC TCTGTCTGGA CGACAAACTT ATCAACTTCC TGCAGCGGAA AGCGCGTTTT
    CCCTCCTTGG GAGATGATAC GTCCAGTTGC TCCGACGCCG CGTCTCCTAA TGTGTCCCAC
    AGGCGGAAGA GCGAAGTGCG CTTTCTGGAG CTGCATCGTG TTGCTTCCTC ATCTACTATG
    AAAAGTTTAT CAGCGGACCC CCCATCTTCC CCAATGATCG AGGTGCTGCA CACCCCTCAG
    TTCCAGATGA GGCGCCTGAA GAAGCAACTG AGTGAAGTGC GGGACTGTAG AGATGAACTG
    GAGGGGGAAC TGGCAGAGAA CCGCAAACAC CTGGCAGAGA AAGAGGCTCA GATCTCCCTC
    CTGCAGCAGC AGATTGAACA CCTTCGGATC TTAAGGGAGA ACCAGGCTGA GCTACAGGAA
    CCCAAAGAGC TGGAGGAACT TCGGGAGAAG AATGAGAGCC TGATGATCAG ACTTCGGGAC
    ACCCTCAAGC AGTGTCAGGA TATGAAGACT GACAGGAAAC AACTGGAACG AAAGAACGAT
    CAATTGGCCG AGGAGAATGG CGAGCTCTCT TACAAGGTGC GAGACTTATC CAACCGCTTG
    GCACAACTGC AGGAGGCTCT CTATGAAACC ACAGAGGAAC ACGAGCTTTC TCTGAGCAAC
    TGGCAGCAGA AACAGAGCCA GTTAGAGAGT GAGCTGAGTG GTGCAGTCGG TGAAAAGAAG
    TATCTGGAAG AGCATCACTT AATACTGCAG GGAAAAATAT CAATGCTTGA GGATCAGCTA
    AAGAAGATGG GGGAGAGAGA GGTGCAAGAA AAAGGAGATT GTATGGGTGA CATCCTCAAG
    CTGGAAGACT TGAAGCAAGA GGTAACTGCG CTCAGCACCC AGTGCCTCAG TCTAACAGAA
    CAGATCCATC AGTTGGAAGA GGAAAAGAGC ACTACAGAAG TTGACATGGA GGCACAGAGG
    TCCAGATTTG AATCTGAGAA AAGTCAGCTG CAGGAGATAG TCACTAACCT GCAGACTTCA
    TTGTCAGAAA TAACATTTCA GAAGGAGAGG CAAGATAACG AGGCCAGGGC ACAACAGGAA
    CATCTGACAT GCCAGATTAC AACATTAAAA CTGGAGATCT CCAAGCTGAA GTCTTCCCTG
    GTGCATAAGG AGGAAGAGCT GAAGGGCATT CACCATCAAG TGGAAGAAGA GAAGAGAGAA
    AAAAATCAGC TCTTGGAAAA CTTCAAAATA CTGGAAGAAT CCTCAAAGCA GAACATTCAG
    GAGCTTGTAA ACCAAGTGGA GCAAATTAGT AGCTCTTTGA AGGTTTCCGA GGGCAATCTG
    GTTGGACTTA CACAACAGCT AGAGAGCAAG GCGAAAGAAG TTGACTATTT AAGAGAAGAA
    CAGCAGAAAG TTGCCTGTGA GAGAGATTCC ACTCTTTCCA CATTCAATGA GTACAAGTGC
    AAAAAGGATG AAGAGTTCAG TGTCTTAAAC ATGACCATGA AAACACTGGA ACAAGATCAT
    CAAGCTAGTC TTTCTGTTAT AGAGGAGCTG AAAAGGGAAA AGGCAGAGCT GGCATCCAAG
    CTACAGGAAC TGGATGCCAC CATTCTAGAT CTGCTTGCTA AATGCCAGAA CTTGGATAGT
    GAAAATGCTT CACAGAGCAA ATCTCATGCA GCCGCTGTGG AGTCTCTAAA GGCACAGCTT
    TCAGAGCAGG AGAGTCAACT GAAAATATAT CAGAAGAAGG TGTCCTCCTG TGAATTGGTG
    TCCGAAGAAA ACTCGCAACT TAAAGAACAG CTCCTGTCTA TGGACGAAAG TCTGAGACAT
    CTTAGGGAAC ATCTAGAGAA AGAGAAAATG AAATTTGCTG CTTCTCTTGA TAGCGACGGT
    AAAAGAATTT CCCACCTTGA AGAGGAGATG AAGAAGTTGA GTGAAAGCAG AGATGCAGCA
    CTGAGTAACC TTGCTGAAGA AAGGGCCGCT GGACAGAAGA TTGAATCTCA GCTGAAGCAC
    TTACAGGGGG AGTACCATAC AGCAAATGAA ACCCTGCAGG CAAAAATGGC AGAATCCAGC
    GCTGCTATCA AACAGCGTGA AGCAGAAAGG GACGAGTTGT CTAAGGTGGT TGACATCTGG
    AAGGCCAAAT ATGAAGAGTC TCAGCAGAAG ATAGTGCAGA ACTCCTGCCA GATGGAGGAA
    CAAATTCAAC ATCTGAAGAA GGCTCATACT GATGTTTGCC AGCAACTGGA GGGAGAGAAG
    AGTAAAGTTT TGATGTTGGA AGCCAAGATA AAGGAAACAA ACTCTTCTCA ACTGGAGCAG
    ATAAATCGGT TGGAGTGTGA ACTGTCTGAA GCCTACTCTC GTATCAAAGA GAGAGAAGCT
    GAGGAAAAGA AGCTACTCTC TGCTCTCCAC TCTTCAGAGG AGAAATTTCA GATTGCTCAT
    CAAGGGGAAA GTGAGCGTCT TTCCCATCTG GAAACAGCCT TAAGCAATGC AAAGTCAGAA
    CTGGATTGTC TAGTCAGAGA GGTGTCTGAT GAGAAGCATA AGAAAGAAGA ACTTGAAGCC
    TTGGTGAAAG AGTTAAAAGA GCAAAAATCT GAAAGAATTC AGTCCCTGGA ATCTGAGGTG
    AAAACCAGTT TAGCTGCTGT TAAAGAGCGA GAAAGTGAAA CTGCAAAGCT TTCTGAAGAG
    GTGAAGGATC TGAACAGGCA GCTAGAGGAG ATCGGTCAGA AGCATAAGGA AGAGCTGGCT
    CAGAAAAATA CTGAAATTGA GCAGTTGATT GAGGCCAAAG AAAAAGCTGC GTTGGACTTG
    GCTAGTAAAA CAGAGATGGG GGCTCAGCTG CAGAAATTGT TGGATACGCA CAAAGGTGAG
    CTCAGTGCCT TGCAGAATGA GCTTTCCCGT TCCCTTGATC TCATCTCTCA GAAGGAAAGT
    GAAGTAGAAA GACTTCATAA AGAGGCTGCC GTAACACAAG AGGAGATACG GAAGCAGCAA
    CAGACTATGG GAAAGCTCAC AGAAGAGCTA ACTGCTTTGG TTGCACTTAA AGAACAGGCA
    GCCCTGCAGG AAAAAGAGAT TGCACAGCAT GTCCGGGCCA CTAAGGGTGC TGAAGCAGAG
    ATGGCTAACC TAAAAGCTAT CATTTCTGAA AAAACTAAAA ATATTGAATC CTTGGAACAC
    GACATCAAGA ACCAGAAGGG AGACCTTGCT TGCATTCAAG AGCAGTATCG GAGCAAGCAG
    GAGGAGGCCC GAGGTCTTCA GGGCCAAATA GTAGATCTGG AAAGAGAGTG CAAAGAGCAG
    AAGGAACTGA TTTGTCAGGC CCAGAAACAG GCTGCTGAGG CAGAAACACT GGCCTCCGAA
    AAGGTTTCAA CGTCGGAGAG ACAGCTAGAA GGAATCCAAG CGCTCGAAGG TGAAATCCAT
    AAAGAGAGGC AAAAGACGTG TGACCTGCTG AAGCAGCTGG AGGCTTCTCA GGCCGCGCAG
    GCAGATAAGG AAACTGAGCT ACAAGCACTG AAGAAAGAGT TGTTTCATAA GGTTCAAGAA
    CTGGAGCAAA GTCAGAAATC TTCTACTGAG TCAAGTCGAG AGCTCTCCTC TATGCTTTCT
    GCGGCTCAGG AGAGGCAGCG AGCCTTAACA GAGGCCAAAC AACAAGCAGA ACAGTACCAG
    AAGGAAATTG AAATGAAGAG TAAAGAGGTG GGCAGCCTGC ATGCAGAAGT GAAGAGCTTG
    AGTTCTAAGG TGGAGGTGTC TGCGGAGTTT GAACAGCGGC TATTGAAGGA AACTAATAGA
    AGCACAAAGC TAGAAGAGAA GCTGAAAAAG CTCCATGGTG ATGTAGAGGC CTCCTCTAAG
    GAGCTACTTG AAAAGAATCG CGCTATGGAT AGTTTAAAGA CGGAAGCCCA AACCTGCAGA
    CAAGAAGCAG ACAAGCAAAG AATGGCAGTT GAGGGCCTCC AGCAGTCGCT CTCCTCCCAG
    GCAGAAACAA ACGATCGCTT GCAGCAGGAA ATTCGCTCCT GGCAGGGAAA CTGTGCTGAG
    AAGGAACAGC AAATCTCTTC TCTCCAACAG AATCTCAGCA GCAACCAAAG CCTCCTGGAG
    GAATTTGCAT CCCTAAAACG CAGTTACCAG GAAATCATTG CAGAACGGGA AGTGATGCAA
    GAGAAGCACC AGGAGGAACT TCTGGGCCAG AAAAAGCTAA CTGAGCGTTT CCAGGCAGAA
    CTGCAAAAGA CTAAGGAGGA CATGGCCGAA ATGGTTCTAC TCAAGGAGAA AGTTCACAAC
    CAAGAGCTTC GGTTACAGAA ACTGCACTCC GAGAACGACG ATCACTTGTC CCAAATAGCC
    CATCTTCAGC AGCTCAACAG CCGGCTCGTT GGGGAGAACC AAAGCTTGTC TCAAGCTTCT
    GACCAGGGTG CCAAGAAACT GGAGAGCGAG GTGTCCAACC TCAAGGAGCA GCACGAGGAA
    CAGCTGAAGT CTCTCCGCCT GCAGCACGAG AAGACTCTGA GAGAAGGCAA CGAGCGAGTA
    CAAGATCTCA GCCAGAAACT GGAAACTGCA ACCGGCAAAT ACGAGCACGT GAAAACGAGG
    GTTGTGGATG ATTGGAAAGC GTTCCAGGAG GAAAAGCAGA GGCTCCTTCT TCAGGTGCAG
    GAGCTGAACA AGCAGCTGAG CCAGCAGGAG AAGGCCTTAC GGAGCCAGCA GCACAAGCTG
    AAGCAGCAGG AAGGTGAGAC TCATGAGGAG GCTGAGAAGG CTCAGAAGCG AGTGGTGGAG
    TTGGAGTCCC AGGTGGAACA GCAGACTCAG GCCGTGGAAC ATTACAAAGC TCAGATGGAG
    AAAGCCAAGC TGTATTACGA TGCCAAGAAG AAGCAGAACC AGGAGCTAAG CGAGGAGCTG
    CAAAGCCACA TTAGAGAGCA GGAACATCTG CGCAAGGAGA ACGTAGATCT AAAGGCAGAG
    TCTGAGCAGC TCAGTAAGGA GCTACAGCAT TCGCTGCTAC AGAACAAAGA GGCAGAGCAA
    AACTGCAAGA ACCTGTCCAA TCGGGTTAGG AGTCTGGAGG CCCAGGTGGA GTATGCCGAC
    CGGCAGCTGC GAGACCTGGG GAAGTTCCAG TTGGCCACGG ATTCCATGAA GAGTCGGGAA
    ACTTTCGGAG CCCCCCGTGT AACACGGAGC CATGCAGATG TCAGTATAGA CAGCCTGGAC
    TTGAGCTTTG AGGATGACCA GCTTTTGAAC TCAACAAGTA AGAATGGCCG CTGCAACGAG
    GAACCTGCCA CATCGTCAGT ACATGCCAGC AGCATGGAGT CTCCTACCAC AGGCCAACTG
    CCCAAAAAGG TGGAGTCTCT GGAAAGCCTC TACTTCACAC CTATTCCAAC CCGGGCACAG
    TCCAAACTGG AAAGTAGCCT TGGTTCCATC GGGGACCTGT CCCTAGACTC CAGCAAGAAA
    ACGCGGTCTG CAAGGAGACG CACCACCCAG ATCATTAACA TCACAATGAC CAAGAAGACG
    AAGGAGGAAC CGGAAGCAGA GAGTGCCAAC ACGTCATTCT ACAGCCTGCG CTCCGCTCCG
    TCCTATCAGA GTCTCCACCA GCAGAAGCCC CGGAGGCCTG GGCGTCCTCA GGCAGCGGTT
    TCTGCCCCAG CCCTGGCCAG CCTCCCATCC CAGGAATCCC TGGCCAAGAC AGAACAGTTC
    TCTGATGACA GCTTTAATAA CTCCGTATTG ATGAATTTAC CTGGTTACCG ACCCCCCGCG
    CGTAGATCTG CAAGGCTTTC ACAAACTGGA GGCCGAAGCA GTTTCTACAT GAGCACGTGC
    CAGGATGAGC CAGACCCACA GGAAGACTGG AACCGGATCG CTGAGTTGCA GGCCCGGAAC
    AAGGCCTGCC CACCCCACCT CAAGACTTCC TACCCACTGG AGTCCAGGCC GTCACTCTTA
    AGCTCCACCA TTACCGATGA AGAGGTCAAG TTGGGAGACC CTAAAGAGAC GCTCAGGAGA
    GCCACCATGC TGCCCTCCCA GATACAAGAG TCCACAGGCA GTACCCGCAG AATGAGCTTG
    GCTGCCTCTG AACATATGAG GGGACCCAGC ATCAGCACCA GGCAGCAGAT GAAGCGCGTG
    TCCGAGGAGT CTCACCTTGG CCCAGACACC CCAGAAGCCA AGAAGTCTGC CACTTGCTTT
    CCTCGGCCCA TGACCCCAAA GGATAAACAC GACGCACGCA GATTGTCCAC TGTGGAAAGC
    AAAGGAAACT CCAGCCATCA GCAGGCGCAG CCCACTCGCC GTCAGTCTAC AGCATTTAGC
    ATTTTCAACA CCCCCAGAAA GCTGGGCAAC AGCCTCCTGA AGAGAGGCCT CAACAAGAAA
    ACGACTCCCA AAAATAGCCC CCGGGGGCGG GGGGCAAGTG GCAGCGCCGG CAGCACTTCA
    GGCAAATCCC CGCACCGCTC TCTCCGGAAG TCACCCAGCA GGCGGTCTCC GAGGGCCTCC
    ACCGCAAAGT CCCCCAAAGC TTCAACCAAG TTTTTCGAGC GCAAGCATCA GAGGAACAAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.