PNPT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PNPT1
Entrez Gene ID	87178
Full Name	polyribonucleotide nucleotidyltransferase 1
Synonyms	COXPD13,DFNB70,OLD35,PNPASE,old-35
General protein information	Preferred Names polyribonucleotide nucleotidyltransferase 1 Names polyribonucleotide nucleotidyltransferase 1, mitochondrial 3'-5' RNA exonuclease OLD35 PNPase 1 PNPase old-35 polynucleotide phosphorylase 1 polynucleotide phosphorylase-like protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p16.1
Summary	The protein encoded by this gene belongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3'-to-5' exoribonucleases implicated in RNA processing and degradation. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7. [provided by RefSeq, Dec 2012].
Disorder MIM:	610316
Disorder Html:	Combined oxidative phosphorylation deficiency 13, 614932 (3); Deafness, autosomal recessive 70, 614934 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_033109.5	NP_149100.2	polyribonucleotide nucleotidyltransferase 1, mitochondrial precursor
XM_005264629.2	XP_005264686.1	polyribonucleotide nucleotidyltransferase 1, mitochondrial isoform X1
XM_017005172.1	XP_016860661.1	polyribonucleotide nucleotidyltransferase 1, mitochondrial isoform X1
NM_033109.4	NP_149100.2	polyribonucleotide nucleotidyltransferase 1, mitochondrial precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PNPT1	XP_538497.3
Bos taurus (cattle)	PNPT1	NP_001179126.1
Drosophila melanogaster (fruit fly)	CG11337	NP_001097990.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010791	XP_318517.3
Homo sapiens (human)	PNPT1	NP_149100.2
	PNPT1	XP_001112421.1
Danio rerio (zebrafish)	pnpt1	NP_001238761.1
Gallus gallus (chicken)	PNPT1	XP_419282.4
Caenorhabditis elegans (roundworm)	BE0003N10.1	NP_497324.3
Xenopus tropicalis (tropical clawed frog)	pnpt1	NP_001017033.1
Pan troglodytes (chimpanzee)	PNPT1	XP_515490.2
Mus musculus (house mouse)	Pnpt1	NP_082145.1
Rattus norvegicus (Norway rat)	Pnpt1	NP_001135843.1
Arabidopsis thaliana (thale cress)	AT5G14580	NP_196962.1

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of homologous phosphorolytic ribonucleases by citrate may represent an evolutionarily conserved communicative link between RNA degradation and central metabolism.
Stone CM, Butt LE, Bufton JC, Lourenco DC, Gowers DM, Pickford AR, Cox PA, Vincent HA, Callaghan AJ
Nucleic acids research45(8)4655-4666(2017 May)

Whole-exome sequencing identifies novel variants in PNPT1 causing oxidative phosphorylation defects and severe multisystem disease.
Alodaib A, Sobreira N, Gold WA, Riley LG, Van Bergen NJ, Wilson MJ, Bennetts B, Thorburn DR, Boehm C, Christodoulou J
European journal of human genetics : EJHG25(1)79-84(2016 01)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The PNPT1 p.Arg136His and p.Pro140Leu variants in our subject showed 1) the mutated amino acids are highly conserved and our structural analysis supported them to be functionally deleterious; 2) The carrier frequencies of the variants are very low in populations (1:60.000), with no homozygous carriers found; 3) The RC complex amounts in the subject's myoblasts were functionally rescued by expression of wild-type PNPT1.
Title: Defective mitochondrial RNA processing due to PNPT1 variants causes Leigh syndrome.

Inhibition of homologous PNPase by citrate may represent an evolutionarily conserved communicative link between RNA degradation and central metabolism.
Title: Inhibition of homologous phosphorolytic ribonucleases by citrate may represent an evolutionarily conserved communicative link between RNA degradation and central metabolism.

In vitro rescue experiments, using exogenous expression of wild-type PNPT1 in patient fibroblasts, ameliorated the deficiencies in the OXPHOS complex protein expression, supporting the likely pathogenicity of these variants and the importance of Whole-exome sequencingin efficiently identifying rare genetic disease genes
Title: Whole-exome sequencing identifies novel variants in PNPT1 causing oxidative phosphorylation defects and severe multisystem disease.

this study provides further evidence that hPNPase(old-35) is associated with global changes in cell cycle-associated genes and identifies potential gene targets for future investigation
Title: Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPase(old-35)).

fresh insight into cellular pathways regulated by PNPT1
Title: Identification of genes potentially regulated by human polynucleotide phosphorylase (hPNPase old-35) using melanoma as a model.

The following PNPT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PNPT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19916	NM_033109.5 Latest version!	Homo sapiens polyribonucleotide nucleotidyltransferase 1 (PNPT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$552.30 $789.00
OHu35647	XM_017005172.1 Latest version!	Homo sapiens polyribonucleotide nucleotidyltransferase 1 (PNPT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu35647	XM_005264629.2 Latest version!	Homo sapiens polyribonucleotide nucleotidyltransferase 1 (PNPT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu19916	NM_033109.4	Homo sapiens polyribonucleotide nucleotidyltransferase 1 (PNPT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$552.30 $789.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PNPT1 ( NM_033109.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_149100.2 Homo sapiens polyribonucleotide nucleotidyltransferase 1 (PNPT1), mRNA.

Price & Availability↑

CloneID	OHu19916
Clone ID Related Accession (Same CDS sequence)	NM_033109.5 , NM_033109.4
Accession Version	NM_033109.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	polyribonucleotide nucleotidyltransferase 1, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB461021.1, AJ458465.1, BC009057.1, CR749867.1, BU943827.1, AW731791.1 and AC015982.9. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_033109.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.107924.1, SRR1803613.224563.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 12798676; reported by MitoCarta MANE Ensembl match :: ENST00000447944.7/ ENSP00000400646.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCCT GCAGGTACTG CTGCTCGTGC CTCCGGCTCC GGCCCCTGAG CGATGGTCCT 
TTCCTTCTGC CACGGCGGGA TCGGGCACTC ACCCAGTTGC AAGTGCGAGC ACTATGGAGT 
AGCGCAGGGT CTCGAGCTGT GGCCGTGGAC TTAGGCAACA GGAAATTAGA AATATCTTCT 
GGAAAGCTGG CCAGATTTGC AGATGGCTCT GCTGTAGTAC AGTCAGGTGA CACTGCAGTA 
ATGGTCACAG CGGTCAGTAA AACAAAACCT TCCCCTTCCC AGTTTATGCC TTTGGTGGTT 
GACTACAGAC AAAAAGCTGC TGCAGCAGGT AGAATTCCCA CAAACTATCT GAGAAGAGAG 
ATTGGTACTT CTGATAAAGA AATTCTAACA AGTCGAATAA TAGATCGTTC AATTAGACCG 
CTCTTTCCAG CTGGCTACTT CTATGATACA CAGGTTCTGT GTAATCTGTT AGCAGTAGAT 
GGTGTAAATG AGCCTGATGT CCTAGCAATT AATGGCGCTT CCGTAGCCCT CTCATTATCA 
GATATTCCTT GGAATGGACC TGTTGGGGCA GTACGAATAG GAATAATTGA TGGAGAATAT 
GTTGTTAACC CAACAAGAAA AGAAATGTCT TCTAGTACTT TAAATTTAGT GGTTGCTGGA 
GCACCTAAAA GTCAGATTGT CATGTTGGAA GCCTCTGCAG AGAACATTTT ACAGCAGGAC 
TTTTGCCATG CTATCAAAGT GGGAGTGAAA TATACCCAAC AAATAATTCA GGGCATTCAG 
CAGTTGGTAA AAGAAACTGG TGTTACCAAG AGGACACCTC AGAAGTTATT TACCCCTTCG 
CCAGAGATTG TGAAATATAC TCATAAACTT GCTATGGAGA GACTCTATGC AGTTTTTACA 
GATTACGAGC ATGACAAAGT TTCCAGAGAT GAAGCTGTTA ACAAAATAAG ATTAGATACG 
GAGGAACAAC TAAAAGAAAA ATTTCCAGAA GCCGATCCAT ATGAAATAAT AGAATCCTTC 
AATGTTGTTG CAAAGGAAGT TTTTAGAAGT ATTGTTTTGA ATGAATACAA AAGGTGCGAT 
GGTCGGGATT TGACTTCACT TAGGAATGTA AGTTGTGAGG TAGATATGTT TAAAACCCTT 
CATGGATCAG CATTATTTCA AAGAGGACAA ACACAGGTGC TTTGTACCGT TACATTTGAT 
TCATTAGAAT CTGGTATTAA GTCAGATCAA GTTATAACAG CTATAAATGG GATAAAAGAT 
AAAAATTTCA TGCTGCACTA CGAGTTTCCT CCTTATGCAA CTAATGAAAT TGGCAAAGTC 
ACTGGTTTAA ATAGAAGAGA ACTTGGGCAT GGTGCTCTTG CTGAGAAAGC TTTGTATCCT 
GTTATTCCCC GAGATTTTCC TTTCACCATA AGAGTTACAT CTGAAGTCCT AGAGTCAAAT 
GGGTCATCTT CTATGGCATC TGCATGTGGC GGAAGTTTAG CATTAATGGA TTCAGGGGTT 
CCAATTTCAT CTGCTGTTGC AGGCGTAGCA ATAGGATTGG TCACCAAAAC CGATCCTGAG 
AAGGGTGAAA TAGAAGATTA TCGTTTGCTG ACAGATATTT TGGGAATTGA AGATTACAAT 
GGTGACATGG ACTTCAAAAT AGCTGGCACT AATAAAGGAA TAACTGCATT ACAGGCTGAT 
ATTAAATTAC CTGGAATACC AATAAAAATT GTGATGGAGG CTATTCAACA AGCTTCAGTG 
GCAAAAAAGG AGATATTACA GATCATGAAC AAAACTATTT CAAAACCTCG AGCATCTAGA 
AAAGAAAATG GACCTGTTGT AGAAACTGTT CAGGTTCCAT TATCAAAACG AGCAAAATTT 
GTTGGACCTG GTGGCTATAA CTTAAAAAAA CTTCAGGCTG AAACAGGTGT AACTATTAGT 
CAGGTGGATG AAGAAACGTT TTCTGTATTT GCACCAACAC CCAGTGCTAT GCATGAGGCA 
AGAGACTTCA TTACTGAAAT CTGCAAGGAT GATCAGGAGC AGCAATTAGA ATTTGGAGCA 
GTATATACCG CCACAATAAC TGAAATCAGA GATACTGGTG TAATGGTAAA ATTATATCCA 
AATATGACTG CGGTACTGCT TCATAACACA CAACTTGATC AACGAAAGAT TAAACATCCT 
ACTGCCCTAG GATTAGAAGT TGGCCAAGAA ATTCAGGTGA AATACTTTGG ACGTGACCCA 
GCCGATGGAA GAATGAGGCT TTCTCGAAAA GTGCTTCAGT CGCCAGCTAC AACCGTGGTC 
AGAACTTTGA ATGACAGAAG TAGTATTGTA ATGGGAGAAC CTATTTCACA GTCATCATCT 
AATTCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149100.2
CDS	22..2373
Translation	MAACRYCCSCLRLRPLSDGPFLLPRRDRALTQLQVRALWSSAGSRAVAVDLGNRKLEISSGKLARFADGSAVVQSGDTAVMVTAVSKTKPSPSQFMPLVVDYRQKAAAAGRIPTNYLRREIGTSDKEILTSRIIDRSIRPLFPAGYFYDTQVLCNLLAVDGVNEPDVLAINGASVALSLSDIPWNGPVGAVRIGIIDGEYVVNPTRKEMSSSTLNLVVAGAPKSQIVMLEASAENILQQDFCHAIKVGVKYTQQIIQGIQQLVKETGVTKRTPQKLFTPSPEIVKYTHKLAMERLYAVFTDYEHDKVSRDEAVNKIRLDTEEQLKEKFPEADPYEIIESFNVVAKEVFRSIVLNEYKRCDGRDLTSLRNVSCEVDMFKTLHGSALFQRGQTQVLCTVTFDSLESGIKSDQVITAINGIKDKNFMLHYEFPPYATNEIGKVTGLNRRELGHGALAEKALYPVIPRDFPFTIRVTSEVLESNGSSSMASACGGSLALMDSGVPISSAVAGVAIGLVTKTDPEKGEIEDYRLLTDILGIEDYNGDMDFKIAGTNKGITALQADIKLPGIPIKIVMEAIQQASVAKKEILQIMNKTISKPRASRKENGPVVETVQVPLSKRAKFVGPGGYNLKKLQAETGVTISQVDEETFSVFAPTPSAMHEARDFITEICKDDQEQQLEFGAVYTATITEIRDTGVMVKLYPNMTAVLLHNTQLDQRKIKHPTALGLEVGQEIQVKYFGRDPADGRMRLSRKVLQSPATTVVRTLNDRSSIVMGEPISQSSSNSQ

Target ORF information:

RefSeq Version	NM_033109.5
Organism	Homo sapiens(human)
Definition	Homo sapiens polyribonucleotide nucleotidyltransferase 1 (PNPT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033109.5

ORF Insert Sequence:

ATGGCGGCCT GCAGGTACTG CTGCTCGTGC CTCCGGCTCC GGCCCCTGAG CGATGGTCCT 
TTCCTTCTGC CACGGCGGGA TCGGGCACTC ACCCAGTTGC AAGTGCGAGC ACTATGGAGT 
AGCGCAGGGT CTCGAGCTGT GGCCGTGGAC TTAGGCAACA GGAAATTAGA AATATCTTCT 
GGAAAGCTGG CCAGATTTGC AGATGGCTCT GCTGTAGTAC AGTCAGGTGA CACTGCAGTA 
ATGGTCACAG CGGTCAGTAA AACAAAACCT TCCCCTTCCC AGTTTATGCC TTTGGTGGTT 
GACTACAGAC AAAAAGCTGC TGCAGCAGGT AGAATTCCCA CAAACTATCT GAGAAGAGAG 
ATTGGTACTT CTGATAAAGA AATTCTAACA AGTCGAATAA TAGATCGTTC AATTAGACCG 
CTCTTTCCAG CTGGCTACTT CTATGATACA CAGGTTCTGT GTAATCTGTT AGCAGTAGAT 
GGTGTAAATG AGCCTGATGT CCTAGCAATT AATGGCGCTT CCGTAGCCCT CTCATTATCA 
GATATTCCTT GGAATGGACC TGTTGGGGCA GTACGAATAG GAATAATTGA TGGAGAATAT 
GTTGTTAACC CAACAAGAAA AGAAATGTCT TCTAGTACTT TAAATTTAGT GGTTGCTGGA 
GCACCTAAAA GTCAGATTGT CATGTTGGAA GCCTCTGCAG AGAACATTTT ACAGCAGGAC 
TTTTGCCATG CTATCAAAGT GGGAGTGAAA TATACCCAAC AAATAATTCA GGGCATTCAG 
CAGTTGGTAA AAGAAACTGG TGTTACCAAG AGGACACCTC AGAAGTTATT TACCCCTTCG 
CCAGAGATTG TGAAATATAC TCATAAACTT GCTATGGAGA GACTCTATGC AGTTTTTACA 
GATTACGAGC ATGACAAAGT TTCCAGAGAT GAAGCTGTTA ACAAAATAAG ATTAGATACG 
GAGGAACAAC TAAAAGAAAA ATTTCCAGAA GCCGATCCAT ATGAAATAAT AGAATCCTTC 
AATGTTGTTG CAAAGGAAGT TTTTAGAAGT ATTGTTTTGA ATGAATACAA AAGGTGCGAT 
GGTCGGGATT TGACTTCACT TAGGAATGTA AGTTGTGAGG TAGATATGTT TAAAACCCTT 
CATGGATCAG CATTATTTCA AAGAGGACAA ACACAGGTGC TTTGTACCGT TACATTTGAT 
TCATTAGAAT CTGGTATTAA GTCAGATCAA GTTATAACAG CTATAAATGG GATAAAAGAT 
AAAAATTTCA TGCTGCACTA CGAGTTTCCT CCTTATGCAA CTAATGAAAT TGGCAAAGTC 
ACTGGTTTAA ATAGAAGAGA ACTTGGGCAT GGTGCTCTTG CTGAGAAAGC TTTGTATCCT 
GTTATTCCCC GAGATTTTCC TTTCACCATA AGAGTTACAT CTGAAGTCCT AGAGTCAAAT 
GGGTCATCTT CTATGGCATC TGCATGTGGC GGAAGTTTAG CATTAATGGA TTCAGGGGTT 
CCAATTTCAT CTGCTGTTGC AGGCGTAGCA ATAGGATTGG TCACCAAAAC CGATCCTGAG 
AAGGGTGAAA TAGAAGATTA TCGTTTGCTG ACAGATATTT TGGGAATTGA AGATTACAAT 
GGTGACATGG ACTTCAAAAT AGCTGGCACT AATAAAGGAA TAACTGCATT ACAGGCTGAT 
ATTAAATTAC CTGGAATACC AATAAAAATT GTGATGGAGG CTATTCAACA AGCTTCAGTG 
GCAAAAAAGG AGATATTACA GATCATGAAC AAAACTATTT CAAAACCTCG AGCATCTAGA 
AAAGAAAATG GACCTGTTGT AGAAACTGTT CAGGTTCCAT TATCAAAACG AGCAAAATTT 
GTTGGACCTG GTGGCTATAA CTTAAAAAAA CTTCAGGCTG AAACAGGTGT AACTATTAGT 
CAGGTGGATG AAGAAACGTT TTCTGTATTT GCACCAACAC CCAGTGCTAT GCATGAGGCA 
AGAGACTTCA TTACTGAAAT CTGCAAGGAT GATCAGGAGC AGCAATTAGA ATTTGGAGCA 
GTATATACCG CCACAATAAC TGAAATCAGA GATACTGGTG TAATGGTAAA ATTATATCCA 
AATATGACTG CGGTACTGCT TCATAACACA CAACTTGATC AACGAAAGAT TAAACATCCT 
ACTGCCCTAG GATTAGAAGT TGGCCAAGAA ATTCAGGTGA AATACTTTGG ACGTGACCCA 
GCCGATGGAA GAATGAGGCT TTCTCGAAAA GTGCTTCAGT CGCCAGCTAC AACCGTGGTC 
AGAACTTTGA ATGACAGAAG TAGTATTGTA ATGGGAGAAC CTATTTCACA GTCATCATCT 
AATTCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPT1 ( NM_033109.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_149100.2 Homo sapiens polyribonucleotide nucleotidyltransferase 1 (PNPT1), mRNA.

Price & Availability↑

CloneID	OHu19916
Clone ID Related Accession (Same CDS sequence)	NM_033109.5 , NM_033109.4
Accession Version	NM_033109.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-13
Organism	Homo sapiens(human)
Product	polyribonucleotide nucleotidyltransferase 1, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB461021.1, AJ458465.1, BC009057.1, CR749867.1, BU943827.1 and AW731791.1. This sequence is a reference standard in the RefSeqGene project. On Dec 12, 2012 this sequence version replaced NM_033109.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.224563.1, SRR1660803.142080.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 12798676; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCCT GCAGGTACTG CTGCTCGTGC CTCCGGCTCC GGCCCCTGAG CGATGGTCCT 
TTCCTTCTGC CACGGCGGGA TCGGGCACTC ACCCAGTTGC AAGTGCGAGC ACTATGGAGT 
AGCGCAGGGT CTCGAGCTGT GGCCGTGGAC TTAGGCAACA GGAAATTAGA AATATCTTCT 
GGAAAGCTGG CCAGATTTGC AGATGGCTCT GCTGTAGTAC AGTCAGGTGA CACTGCAGTA 
ATGGTCACAG CGGTCAGTAA AACAAAACCT TCCCCTTCCC AGTTTATGCC TTTGGTGGTT 
GACTACAGAC AAAAAGCTGC TGCAGCAGGT AGAATTCCCA CAAACTATCT GAGAAGAGAG 
ATTGGTACTT CTGATAAAGA AATTCTAACA AGTCGAATAA TAGATCGTTC AATTAGACCG 
CTCTTTCCAG CTGGCTACTT CTATGATACA CAGGTTCTGT GTAATCTGTT AGCAGTAGAT 
GGTGTAAATG AGCCTGATGT CCTAGCAATT AATGGCGCTT CCGTAGCCCT CTCATTATCA 
GATATTCCTT GGAATGGACC TGTTGGGGCA GTACGAATAG GAATAATTGA TGGAGAATAT 
GTTGTTAACC CAACAAGAAA AGAAATGTCT TCTAGTACTT TAAATTTAGT GGTTGCTGGA 
GCACCTAAAA GTCAGATTGT CATGTTGGAA GCCTCTGCAG AGAACATTTT ACAGCAGGAC 
TTTTGCCATG CTATCAAAGT GGGAGTGAAA TATACCCAAC AAATAATTCA GGGCATTCAG 
CAGTTGGTAA AAGAAACTGG TGTTACCAAG AGGACACCTC AGAAGTTATT TACCCCTTCG 
CCAGAGATTG TGAAATATAC TCATAAACTT GCTATGGAGA GACTCTATGC AGTTTTTACA 
GATTACGAGC ATGACAAAGT TTCCAGAGAT GAAGCTGTTA ACAAAATAAG ATTAGATACG 
GAGGAACAAC TAAAAGAAAA ATTTCCAGAA GCCGATCCAT ATGAAATAAT AGAATCCTTC 
AATGTTGTTG CAAAGGAAGT TTTTAGAAGT ATTGTTTTGA ATGAATACAA AAGGTGCGAT 
GGTCGGGATT TGACTTCACT TAGGAATGTA AGTTGTGAGG TAGATATGTT TAAAACCCTT 
CATGGATCAG CATTATTTCA AAGAGGACAA ACACAGGTGC TTTGTACCGT TACATTTGAT 
TCATTAGAAT CTGGTATTAA GTCAGATCAA GTTATAACAG CTATAAATGG GATAAAAGAT 
AAAAATTTCA TGCTGCACTA CGAGTTTCCT CCTTATGCAA CTAATGAAAT TGGCAAAGTC 
ACTGGTTTAA ATAGAAGAGA ACTTGGGCAT GGTGCTCTTG CTGAGAAAGC TTTGTATCCT 
GTTATTCCCC GAGATTTTCC TTTCACCATA AGAGTTACAT CTGAAGTCCT AGAGTCAAAT 
GGGTCATCTT CTATGGCATC TGCATGTGGC GGAAGTTTAG CATTAATGGA TTCAGGGGTT 
CCAATTTCAT CTGCTGTTGC AGGCGTAGCA ATAGGATTGG TCACCAAAAC CGATCCTGAG 
AAGGGTGAAA TAGAAGATTA TCGTTTGCTG ACAGATATTT TGGGAATTGA AGATTACAAT 
GGTGACATGG ACTTCAAAAT AGCTGGCACT AATAAAGGAA TAACTGCATT ACAGGCTGAT 
ATTAAATTAC CTGGAATACC AATAAAAATT GTGATGGAGG CTATTCAACA AGCTTCAGTG 
GCAAAAAAGG AGATATTACA GATCATGAAC AAAACTATTT CAAAACCTCG AGCATCTAGA 
AAAGAAAATG GACCTGTTGT AGAAACTGTT CAGGTTCCAT TATCAAAACG AGCAAAATTT 
GTTGGACCTG GTGGCTATAA CTTAAAAAAA CTTCAGGCTG AAACAGGTGT AACTATTAGT 
CAGGTGGATG AAGAAACGTT TTCTGTATTT GCACCAACAC CCAGTGCTAT GCATGAGGCA 
AGAGACTTCA TTACTGAAAT CTGCAAGGAT GATCAGGAGC AGCAATTAGA ATTTGGAGCA 
GTATATACCG CCACAATAAC TGAAATCAGA GATACTGGTG TAATGGTAAA ATTATATCCA 
AATATGACTG CGGTACTGCT TCATAACACA CAACTTGATC AACGAAAGAT TAAACATCCT 
ACTGCCCTAG GATTAGAAGT TGGCCAAGAA ATTCAGGTGA AATACTTTGG ACGTGACCCA 
GCCGATGGAA GAATGAGGCT TTCTCGAAAA GTGCTTCAGT CGCCAGCTAC AACCGTGGTC 
AGAACTTTGA ATGACAGAAG TAGTATTGTA ATGGGAGAAC CTATTTCACA GTCATCATCT 
AATTCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149100.2
CDS	88..2439
Translation	MAACRYCCSCLRLRPLSDGPFLLPRRDRALTQLQVRALWSSAGSRAVAVDLGNRKLEISSGKLARFADGSAVVQSGDTAVMVTAVSKTKPSPSQFMPLVVDYRQKAAAAGRIPTNYLRREIGTSDKEILTSRIIDRSIRPLFPAGYFYDTQVLCNLLAVDGVNEPDVLAINGASVALSLSDIPWNGPVGAVRIGIIDGEYVVNPTRKEMSSSTLNLVVAGAPKSQIVMLEASAENILQQDFCHAIKVGVKYTQQIIQGIQQLVKETGVTKRTPQKLFTPSPEIVKYTHKLAMERLYAVFTDYEHDKVSRDEAVNKIRLDTEEQLKEKFPEADPYEIIESFNVVAKEVFRSIVLNEYKRCDGRDLTSLRNVSCEVDMFKTLHGSALFQRGQTQVLCTVTFDSLESGIKSDQVITAINGIKDKNFMLHYEFPPYATNEIGKVTGLNRRELGHGALAEKALYPVIPRDFPFTIRVTSEVLESNGSSSMASACGGSLALMDSGVPISSAVAGVAIGLVTKTDPEKGEIEDYRLLTDILGIEDYNGDMDFKIAGTNKGITALQADIKLPGIPIKIVMEAIQQASVAKKEILQIMNKTISKPRASRKENGPVVETVQVPLSKRAKFVGPGGYNLKKLQAETGVTISQVDEETFSVFAPTPSAMHEARDFITEICKDDQEQQLEFGAVYTATITEIRDTGVMVKLYPNMTAVLLHNTQLDQRKIKHPTALGLEVGQEIQVKYFGRDPADGRMRLSRKVLQSPATTVVRTLNDRSSIVMGEPISQSSSNSQ

Target ORF information:

RefSeq Version	NM_033109.4
Organism	Homo sapiens(human)
Definition	Homo sapiens polyribonucleotide nucleotidyltransferase 1 (PNPT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033109.4

ORF Insert Sequence:

ATGGCGGCCT GCAGGTACTG CTGCTCGTGC CTCCGGCTCC GGCCCCTGAG CGATGGTCCT 
TTCCTTCTGC CACGGCGGGA TCGGGCACTC ACCCAGTTGC AAGTGCGAGC ACTATGGAGT 
AGCGCAGGGT CTCGAGCTGT GGCCGTGGAC TTAGGCAACA GGAAATTAGA AATATCTTCT 
GGAAAGCTGG CCAGATTTGC AGATGGCTCT GCTGTAGTAC AGTCAGGTGA CACTGCAGTA 
ATGGTCACAG CGGTCAGTAA AACAAAACCT TCCCCTTCCC AGTTTATGCC TTTGGTGGTT 
GACTACAGAC AAAAAGCTGC TGCAGCAGGT AGAATTCCCA CAAACTATCT GAGAAGAGAG 
ATTGGTACTT CTGATAAAGA AATTCTAACA AGTCGAATAA TAGATCGTTC AATTAGACCG 
CTCTTTCCAG CTGGCTACTT CTATGATACA CAGGTTCTGT GTAATCTGTT AGCAGTAGAT 
GGTGTAAATG AGCCTGATGT CCTAGCAATT AATGGCGCTT CCGTAGCCCT CTCATTATCA 
GATATTCCTT GGAATGGACC TGTTGGGGCA GTACGAATAG GAATAATTGA TGGAGAATAT 
GTTGTTAACC CAACAAGAAA AGAAATGTCT TCTAGTACTT TAAATTTAGT GGTTGCTGGA 
GCACCTAAAA GTCAGATTGT CATGTTGGAA GCCTCTGCAG AGAACATTTT ACAGCAGGAC 
TTTTGCCATG CTATCAAAGT GGGAGTGAAA TATACCCAAC AAATAATTCA GGGCATTCAG 
CAGTTGGTAA AAGAAACTGG TGTTACCAAG AGGACACCTC AGAAGTTATT TACCCCTTCG 
CCAGAGATTG TGAAATATAC TCATAAACTT GCTATGGAGA GACTCTATGC AGTTTTTACA 
GATTACGAGC ATGACAAAGT TTCCAGAGAT GAAGCTGTTA ACAAAATAAG ATTAGATACG 
GAGGAACAAC TAAAAGAAAA ATTTCCAGAA GCCGATCCAT ATGAAATAAT AGAATCCTTC 
AATGTTGTTG CAAAGGAAGT TTTTAGAAGT ATTGTTTTGA ATGAATACAA AAGGTGCGAT 
GGTCGGGATT TGACTTCACT TAGGAATGTA AGTTGTGAGG TAGATATGTT TAAAACCCTT 
CATGGATCAG CATTATTTCA AAGAGGACAA ACACAGGTGC TTTGTACCGT TACATTTGAT 
TCATTAGAAT CTGGTATTAA GTCAGATCAA GTTATAACAG CTATAAATGG GATAAAAGAT 
AAAAATTTCA TGCTGCACTA CGAGTTTCCT CCTTATGCAA CTAATGAAAT TGGCAAAGTC 
ACTGGTTTAA ATAGAAGAGA ACTTGGGCAT GGTGCTCTTG CTGAGAAAGC TTTGTATCCT 
GTTATTCCCC GAGATTTTCC TTTCACCATA AGAGTTACAT CTGAAGTCCT AGAGTCAAAT 
GGGTCATCTT CTATGGCATC TGCATGTGGC GGAAGTTTAG CATTAATGGA TTCAGGGGTT 
CCAATTTCAT CTGCTGTTGC AGGCGTAGCA ATAGGATTGG TCACCAAAAC CGATCCTGAG 
AAGGGTGAAA TAGAAGATTA TCGTTTGCTG ACAGATATTT TGGGAATTGA AGATTACAAT 
GGTGACATGG ACTTCAAAAT AGCTGGCACT AATAAAGGAA TAACTGCATT ACAGGCTGAT 
ATTAAATTAC CTGGAATACC AATAAAAATT GTGATGGAGG CTATTCAACA AGCTTCAGTG 
GCAAAAAAGG AGATATTACA GATCATGAAC AAAACTATTT CAAAACCTCG AGCATCTAGA 
AAAGAAAATG GACCTGTTGT AGAAACTGTT CAGGTTCCAT TATCAAAACG AGCAAAATTT 
GTTGGACCTG GTGGCTATAA CTTAAAAAAA CTTCAGGCTG AAACAGGTGT AACTATTAGT 
CAGGTGGATG AAGAAACGTT TTCTGTATTT GCACCAACAC CCAGTGCTAT GCATGAGGCA 
AGAGACTTCA TTACTGAAAT CTGCAAGGAT GATCAGGAGC AGCAATTAGA ATTTGGAGCA 
GTATATACCG CCACAATAAC TGAAATCAGA GATACTGGTG TAATGGTAAA ATTATATCCA 
AATATGACTG CGGTACTGCT TCATAACACA CAACTTGATC AACGAAAGAT TAAACATCCT 
ACTGCCCTAG GATTAGAAGT TGGCCAAGAA ATTCAGGTGA AATACTTTGG ACGTGACCCA 
GCCGATGGAA GAATGAGGCT TTCTCGAAAA GTGCTTCAGT CGCCAGCTAC AACCGTGGTC 
AGAACTTTGA ATGACAGAAG TAGTATTGTA ATGGGAGAAC CTATTTCACA GTCATCATCT 
AATTCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPT1 ( XM_005264629.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264686.1 Homo sapiens polyribonucleotide nucleotidyltransferase 1 (PNPT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu35647
Clone ID Related Accession (Same CDS sequence)	XM_005264629.2 , XM_017005172.1
Accession Version	XM_005264629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	polyribonucleotide nucleotidyltransferase 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005264629.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCACAG CGGTCAGTAA AACAAAACCT TCCCCTTCCC AGTTTATGCC TTTGGTGGTT 
GACTACAGAC AAAAAGCTGC TGCAGCAGGT AGAATTCCCA CAAACTATCT GAGAAGAGAG 
ATTGGTACTT CTGATAAAGA AATTCTAACA AGTCGAATAA TAGATCGTTC AATTAGACCG 
CTCTTTCCAG CTGGCTACTT CTATGATACA CAGGTTCTGT GTAATCTGTT AGCAGTAGAT 
GGTGTAAATG AGCCTGATGT CCTAGCAATT AATGGCGCTT CCGTAGCCCT CTCATTATCA 
GATATTCCTT GGAATGGACC TGTTGGGGCA GTACGAATAG GAATAATTGA TGGAGAATAT 
GTTGTTAACC CAACAAGAAA AGAAATGTCT TCTAGTACTT TAAATTTAGT GGTTGCTGGA 
GCACCTAAAA GTCAGATTGT CATGTTGGAA GCCTCTGCAG AGAACATTTT ACAGCAGGAC 
TTTTGCCATG CTATCAAAGT GGGAGTGAAA TATACCCAAC AAATAATTCA GGGCATTCAG 
CAGTTGGTAA AAGAAACTGG TGTTACCAAG AGGACACCTC AGAAGTTATT TACCCCTTCG 
CCAGAGATTG TGAAATATAC TCATAAACTT GCTATGGAGA GACTCTATGC AGTTTTTACA 
GATTACGAGC ATGACAAAGT TTCCAGAGAT GAAGCTGTTA ACAAAATAAG ATTAGATACG 
GAGGAACAAC TAAAAGAAAA ATTTCCAGAA GCCGATCCAT ATGAAATAAT AGAATCCTTC 
AATGTTGTTG CAAAGGAAGT TTTTAGAAGT ATTGTTTTGA ATGAATACAA AAGGTGCGAT 
GGTCGGGATT TGACTTCACT TAGGAATGTA AGTTGTGAGG TAGATATGTT TAAAACCCTT 
CATGGATCAG CATTATTTCA AAGAGGACAA ACACAGGTGC TTTGTACCGT TACATTTGAT 
TCATTAGAAT CTGGTATTAA GTCAGATCAA GTTATAACAG CTATAAATGG GATAAAAGAT 
AAAAATTTCA TGCTGCACTA CGAGTTTCCT CCTTATGCAA CTAATGAAAT TGGCAAAGTC 
ACTGGTTTAA ATAGAAGAGA ACTTGGGCAT GGTGCTCTTG CTGAGAAAGC TTTGTATCCT 
GTTATTCCCC GAGATTTTCC TTTCACCATA AGAGTTACAT CTGAAGTCCT AGAGTCAAAT 
GGGTCATCTT CTATGGCATC TGCATGTGGC GGAAGTTTAG CATTAATGGA TTCAGGGGTT 
CCAATTTCAT CTGCTGTTGC AGGCGTAGCA ATAGGATTGG TCACCAAAAC CGATCCTGAG 
AAGGGTGAAA TAGAAGATTA TCGTTTGCTG ACAGATATTT TGGGAATTGA AGATTACAAT 
GGTGACATGG ACTTCAAAAT AGCTGGCACT AATAAAGGAA TAACTGCATT ACAGGCTGAT 
ATTAAATTAC CTGGAATACC AATAAAAATT GTGATGGAGG CTATTCAACA AGCTTCAGTG 
GCAAAAAAGG AGATATTACA GATCATGAAC AAAACTATTT CAAAACCTCG AGCATCTAGA 
AAAGAAAATG GACCTGTTGT AGAAACTGTT CAGGTTCCAT TATCAAAACG AGCAAAATTT 
GTTGGACCTG GTGGCTATAA CTTAAAAAAA CTTCAGGCTG AAACAGGTGT AACTATTAGT 
CAGGTGGATG AAGAAACGTT TTCTGTATTT GCACCAACAC CCAGTGCTAT GCATGAGGCA 
AGAGACTTCA TTACTGAAAT CTGCAAGGAT GATCAGGAGC AGCAATTAGA ATTTGGAGCA 
GTATATACCG CCACAATAAC TGAAATCAGA GATACTGGTG TAATGGTAAA ATTATATCCA 
AATATGACTG CGGTACTGCT TCATAACACA CAACTTGATC AACGAAAGAT TAAACATCCT 
ACTGCCCTAG GATTAGAAGT TGGCCAAGAA ATTCAGGTGA AATACTTTGG ACGTGACCCA 
GCCGATGGAA GAATGAGGCT TTCTCGAAAA GTGCTTCAGT CGCCAGCTAC AACCGTGGTC 
AGAACTTTGA ATGACAGAAG TAGTATTGTA ATGGGAGAAC CTATTTCACA GTCATCATCT 
AATTCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264686.1
CDS	258..2369
Translation	MVTAVSKTKPSPSQFMPLVVDYRQKAAAAGRIPTNYLRREIGTSDKEILTSRIIDRSIRPLFPAGYFYDTQVLCNLLAVDGVNEPDVLAINGASVALSLSDIPWNGPVGAVRIGIIDGEYVVNPTRKEMSSSTLNLVVAGAPKSQIVMLEASAENILQQDFCHAIKVGVKYTQQIIQGIQQLVKETGVTKRTPQKLFTPSPEIVKYTHKLAMERLYAVFTDYEHDKVSRDEAVNKIRLDTEEQLKEKFPEADPYEIIESFNVVAKEVFRSIVLNEYKRCDGRDLTSLRNVSCEVDMFKTLHGSALFQRGQTQVLCTVTFDSLESGIKSDQVITAINGIKDKNFMLHYEFPPYATNEIGKVTGLNRRELGHGALAEKALYPVIPRDFPFTIRVTSEVLESNGSSSMASACGGSLALMDSGVPISSAVAGVAIGLVTKTDPEKGEIEDYRLLTDILGIEDYNGDMDFKIAGTNKGITALQADIKLPGIPIKIVMEAIQQASVAKKEILQIMNKTISKPRASRKENGPVVETVQVPLSKRAKFVGPGGYNLKKLQAETGVTISQVDEETFSVFAPTPSAMHEARDFITEICKDDQEQQLEFGAVYTATITEIRDTGVMVKLYPNMTAVLLHNTQLDQRKIKHPTALGLEVGQEIQVKYFGRDPADGRMRLSRKVLQSPATTVVRTLNDRSSIVMGEPISQSSSNSQ

Target ORF information:

RefSeq Version	XM_005264629.2
Organism	Homo sapiens(human)
Definition	Homo sapiens polyribonucleotide nucleotidyltransferase 1 (PNPT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264629.2

ORF Insert Sequence:

ATGGTCACAG CGGTCAGTAA AACAAAACCT TCCCCTTCCC AGTTTATGCC TTTGGTGGTT 
GACTACAGAC AAAAAGCTGC TGCAGCAGGT AGAATTCCCA CAAACTATCT GAGAAGAGAG 
ATTGGTACTT CTGATAAAGA AATTCTAACA AGTCGAATAA TAGATCGTTC AATTAGACCG 
CTCTTTCCAG CTGGCTACTT CTATGATACA CAGGTTCTGT GTAATCTGTT AGCAGTAGAT 
GGTGTAAATG AGCCTGATGT CCTAGCAATT AATGGCGCTT CCGTAGCCCT CTCATTATCA 
GATATTCCTT GGAATGGACC TGTTGGGGCA GTACGAATAG GAATAATTGA TGGAGAATAT 
GTTGTTAACC CAACAAGAAA AGAAATGTCT TCTAGTACTT TAAATTTAGT GGTTGCTGGA 
GCACCTAAAA GTCAGATTGT CATGTTGGAA GCCTCTGCAG AGAACATTTT ACAGCAGGAC 
TTTTGCCATG CTATCAAAGT GGGAGTGAAA TATACCCAAC AAATAATTCA GGGCATTCAG 
CAGTTGGTAA AAGAAACTGG TGTTACCAAG AGGACACCTC AGAAGTTATT TACCCCTTCG 
CCAGAGATTG TGAAATATAC TCATAAACTT GCTATGGAGA GACTCTATGC AGTTTTTACA 
GATTACGAGC ATGACAAAGT TTCCAGAGAT GAAGCTGTTA ACAAAATAAG ATTAGATACG 
GAGGAACAAC TAAAAGAAAA ATTTCCAGAA GCCGATCCAT ATGAAATAAT AGAATCCTTC 
AATGTTGTTG CAAAGGAAGT TTTTAGAAGT ATTGTTTTGA ATGAATACAA AAGGTGCGAT 
GGTCGGGATT TGACTTCACT TAGGAATGTA AGTTGTGAGG TAGATATGTT TAAAACCCTT 
CATGGATCAG CATTATTTCA AAGAGGACAA ACACAGGTGC TTTGTACCGT TACATTTGAT 
TCATTAGAAT CTGGTATTAA GTCAGATCAA GTTATAACAG CTATAAATGG GATAAAAGAT 
AAAAATTTCA TGCTGCACTA CGAGTTTCCT CCTTATGCAA CTAATGAAAT TGGCAAAGTC 
ACTGGTTTAA ATAGAAGAGA ACTTGGGCAT GGTGCTCTTG CTGAGAAAGC TTTGTATCCT 
GTTATTCCCC GAGATTTTCC TTTCACCATA AGAGTTACAT CTGAAGTCCT AGAGTCAAAT 
GGGTCATCTT CTATGGCATC TGCATGTGGC GGAAGTTTAG CATTAATGGA TTCAGGGGTT 
CCAATTTCAT CTGCTGTTGC AGGCGTAGCA ATAGGATTGG TCACCAAAAC CGATCCTGAG 
AAGGGTGAAA TAGAAGATTA TCGTTTGCTG ACAGATATTT TGGGAATTGA AGATTACAAT 
GGTGACATGG ACTTCAAAAT AGCTGGCACT AATAAAGGAA TAACTGCATT ACAGGCTGAT 
ATTAAATTAC CTGGAATACC AATAAAAATT GTGATGGAGG CTATTCAACA AGCTTCAGTG 
GCAAAAAAGG AGATATTACA GATCATGAAC AAAACTATTT CAAAACCTCG AGCATCTAGA 
AAAGAAAATG GACCTGTTGT AGAAACTGTT CAGGTTCCAT TATCAAAACG AGCAAAATTT 
GTTGGACCTG GTGGCTATAA CTTAAAAAAA CTTCAGGCTG AAACAGGTGT AACTATTAGT 
CAGGTGGATG AAGAAACGTT TTCTGTATTT GCACCAACAC CCAGTGCTAT GCATGAGGCA 
AGAGACTTCA TTACTGAAAT CTGCAAGGAT GATCAGGAGC AGCAATTAGA ATTTGGAGCA 
GTATATACCG CCACAATAAC TGAAATCAGA GATACTGGTG TAATGGTAAA ATTATATCCA 
AATATGACTG CGGTACTGCT TCATAACACA CAACTTGATC AACGAAAGAT TAAACATCCT 
ACTGCCCTAG GATTAGAAGT TGGCCAAGAA ATTCAGGTGA AATACTTTGG ACGTGACCCA 
GCCGATGGAA GAATGAGGCT TTCTCGAAAA GTGCTTCAGT CGCCAGCTAC AACCGTGGTC 
AGAACTTTGA ATGACAGAAG TAGTATTGTA ATGGGAGAAC CTATTTCACA GTCATCATCT 
AATTCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPT1 ( XM_017005172.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860661.1 Homo sapiens polyribonucleotide nucleotidyltransferase 1 (PNPT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu35647
Clone ID Related Accession (Same CDS sequence)	XM_005264629.2 , XM_017005172.1
Accession Version	XM_017005172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	polyribonucleotide nucleotidyltransferase 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCACAG CGGTCAGTAA AACAAAACCT TCCCCTTCCC AGTTTATGCC TTTGGTGGTT 
GACTACAGAC AAAAAGCTGC TGCAGCAGGT AGAATTCCCA CAAACTATCT GAGAAGAGAG 
ATTGGTACTT CTGATAAAGA AATTCTAACA AGTCGAATAA TAGATCGTTC AATTAGACCG 
CTCTTTCCAG CTGGCTACTT CTATGATACA CAGGTTCTGT GTAATCTGTT AGCAGTAGAT 
GGTGTAAATG AGCCTGATGT CCTAGCAATT AATGGCGCTT CCGTAGCCCT CTCATTATCA 
GATATTCCTT GGAATGGACC TGTTGGGGCA GTACGAATAG GAATAATTGA TGGAGAATAT 
GTTGTTAACC CAACAAGAAA AGAAATGTCT TCTAGTACTT TAAATTTAGT GGTTGCTGGA 
GCACCTAAAA GTCAGATTGT CATGTTGGAA GCCTCTGCAG AGAACATTTT ACAGCAGGAC 
TTTTGCCATG CTATCAAAGT GGGAGTGAAA TATACCCAAC AAATAATTCA GGGCATTCAG 
CAGTTGGTAA AAGAAACTGG TGTTACCAAG AGGACACCTC AGAAGTTATT TACCCCTTCG 
CCAGAGATTG TGAAATATAC TCATAAACTT GCTATGGAGA GACTCTATGC AGTTTTTACA 
GATTACGAGC ATGACAAAGT TTCCAGAGAT GAAGCTGTTA ACAAAATAAG ATTAGATACG 
GAGGAACAAC TAAAAGAAAA ATTTCCAGAA GCCGATCCAT ATGAAATAAT AGAATCCTTC 
AATGTTGTTG CAAAGGAAGT TTTTAGAAGT ATTGTTTTGA ATGAATACAA AAGGTGCGAT 
GGTCGGGATT TGACTTCACT TAGGAATGTA AGTTGTGAGG TAGATATGTT TAAAACCCTT 
CATGGATCAG CATTATTTCA AAGAGGACAA ACACAGGTGC TTTGTACCGT TACATTTGAT 
TCATTAGAAT CTGGTATTAA GTCAGATCAA GTTATAACAG CTATAAATGG GATAAAAGAT 
AAAAATTTCA TGCTGCACTA CGAGTTTCCT CCTTATGCAA CTAATGAAAT TGGCAAAGTC 
ACTGGTTTAA ATAGAAGAGA ACTTGGGCAT GGTGCTCTTG CTGAGAAAGC TTTGTATCCT 
GTTATTCCCC GAGATTTTCC TTTCACCATA AGAGTTACAT CTGAAGTCCT AGAGTCAAAT 
GGGTCATCTT CTATGGCATC TGCATGTGGC GGAAGTTTAG CATTAATGGA TTCAGGGGTT 
CCAATTTCAT CTGCTGTTGC AGGCGTAGCA ATAGGATTGG TCACCAAAAC CGATCCTGAG 
AAGGGTGAAA TAGAAGATTA TCGTTTGCTG ACAGATATTT TGGGAATTGA AGATTACAAT 
GGTGACATGG ACTTCAAAAT AGCTGGCACT AATAAAGGAA TAACTGCATT ACAGGCTGAT 
ATTAAATTAC CTGGAATACC AATAAAAATT GTGATGGAGG CTATTCAACA AGCTTCAGTG 
GCAAAAAAGG AGATATTACA GATCATGAAC AAAACTATTT CAAAACCTCG AGCATCTAGA 
AAAGAAAATG GACCTGTTGT AGAAACTGTT CAGGTTCCAT TATCAAAACG AGCAAAATTT 
GTTGGACCTG GTGGCTATAA CTTAAAAAAA CTTCAGGCTG AAACAGGTGT AACTATTAGT 
CAGGTGGATG AAGAAACGTT TTCTGTATTT GCACCAACAC CCAGTGCTAT GCATGAGGCA 
AGAGACTTCA TTACTGAAAT CTGCAAGGAT GATCAGGAGC AGCAATTAGA ATTTGGAGCA 
GTATATACCG CCACAATAAC TGAAATCAGA GATACTGGTG TAATGGTAAA ATTATATCCA 
AATATGACTG CGGTACTGCT TCATAACACA CAACTTGATC AACGAAAGAT TAAACATCCT 
ACTGCCCTAG GATTAGAAGT TGGCCAAGAA ATTCAGGTGA AATACTTTGG ACGTGACCCA 
GCCGATGGAA GAATGAGGCT TTCTCGAAAA GTGCTTCAGT CGCCAGCTAC AACCGTGGTC 
AGAACTTTGA ATGACAGAAG TAGTATTGTA ATGGGAGAAC CTATTTCACA GTCATCATCT 
AATTCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860661.1
CDS	208..2319
Translation	MVTAVSKTKPSPSQFMPLVVDYRQKAAAAGRIPTNYLRREIGTSDKEILTSRIIDRSIRPLFPAGYFYDTQVLCNLLAVDGVNEPDVLAINGASVALSLSDIPWNGPVGAVRIGIIDGEYVVNPTRKEMSSSTLNLVVAGAPKSQIVMLEASAENILQQDFCHAIKVGVKYTQQIIQGIQQLVKETGVTKRTPQKLFTPSPEIVKYTHKLAMERLYAVFTDYEHDKVSRDEAVNKIRLDTEEQLKEKFPEADPYEIIESFNVVAKEVFRSIVLNEYKRCDGRDLTSLRNVSCEVDMFKTLHGSALFQRGQTQVLCTVTFDSLESGIKSDQVITAINGIKDKNFMLHYEFPPYATNEIGKVTGLNRRELGHGALAEKALYPVIPRDFPFTIRVTSEVLESNGSSSMASACGGSLALMDSGVPISSAVAGVAIGLVTKTDPEKGEIEDYRLLTDILGIEDYNGDMDFKIAGTNKGITALQADIKLPGIPIKIVMEAIQQASVAKKEILQIMNKTISKPRASRKENGPVVETVQVPLSKRAKFVGPGGYNLKKLQAETGVTISQVDEETFSVFAPTPSAMHEARDFITEICKDDQEQQLEFGAVYTATITEIRDTGVMVKLYPNMTAVLLHNTQLDQRKIKHPTALGLEVGQEIQVKYFGRDPADGRMRLSRKVLQSPATTVVRTLNDRSSIVMGEPISQSSSNSQ

Target ORF information:

RefSeq Version	XM_017005172.1
Organism	Homo sapiens(human)
Definition	Homo sapiens polyribonucleotide nucleotidyltransferase 1 (PNPT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005172.1

ORF Insert Sequence:

ATGGTCACAG CGGTCAGTAA AACAAAACCT TCCCCTTCCC AGTTTATGCC TTTGGTGGTT 
GACTACAGAC AAAAAGCTGC TGCAGCAGGT AGAATTCCCA CAAACTATCT GAGAAGAGAG 
ATTGGTACTT CTGATAAAGA AATTCTAACA AGTCGAATAA TAGATCGTTC AATTAGACCG 
CTCTTTCCAG CTGGCTACTT CTATGATACA CAGGTTCTGT GTAATCTGTT AGCAGTAGAT 
GGTGTAAATG AGCCTGATGT CCTAGCAATT AATGGCGCTT CCGTAGCCCT CTCATTATCA 
GATATTCCTT GGAATGGACC TGTTGGGGCA GTACGAATAG GAATAATTGA TGGAGAATAT 
GTTGTTAACC CAACAAGAAA AGAAATGTCT TCTAGTACTT TAAATTTAGT GGTTGCTGGA 
GCACCTAAAA GTCAGATTGT CATGTTGGAA GCCTCTGCAG AGAACATTTT ACAGCAGGAC 
TTTTGCCATG CTATCAAAGT GGGAGTGAAA TATACCCAAC AAATAATTCA GGGCATTCAG 
CAGTTGGTAA AAGAAACTGG TGTTACCAAG AGGACACCTC AGAAGTTATT TACCCCTTCG 
CCAGAGATTG TGAAATATAC TCATAAACTT GCTATGGAGA GACTCTATGC AGTTTTTACA 
GATTACGAGC ATGACAAAGT TTCCAGAGAT GAAGCTGTTA ACAAAATAAG ATTAGATACG 
GAGGAACAAC TAAAAGAAAA ATTTCCAGAA GCCGATCCAT ATGAAATAAT AGAATCCTTC 
AATGTTGTTG CAAAGGAAGT TTTTAGAAGT ATTGTTTTGA ATGAATACAA AAGGTGCGAT 
GGTCGGGATT TGACTTCACT TAGGAATGTA AGTTGTGAGG TAGATATGTT TAAAACCCTT 
CATGGATCAG CATTATTTCA AAGAGGACAA ACACAGGTGC TTTGTACCGT TACATTTGAT 
TCATTAGAAT CTGGTATTAA GTCAGATCAA GTTATAACAG CTATAAATGG GATAAAAGAT 
AAAAATTTCA TGCTGCACTA CGAGTTTCCT CCTTATGCAA CTAATGAAAT TGGCAAAGTC 
ACTGGTTTAA ATAGAAGAGA ACTTGGGCAT GGTGCTCTTG CTGAGAAAGC TTTGTATCCT 
GTTATTCCCC GAGATTTTCC TTTCACCATA AGAGTTACAT CTGAAGTCCT AGAGTCAAAT 
GGGTCATCTT CTATGGCATC TGCATGTGGC GGAAGTTTAG CATTAATGGA TTCAGGGGTT 
CCAATTTCAT CTGCTGTTGC AGGCGTAGCA ATAGGATTGG TCACCAAAAC CGATCCTGAG 
AAGGGTGAAA TAGAAGATTA TCGTTTGCTG ACAGATATTT TGGGAATTGA AGATTACAAT 
GGTGACATGG ACTTCAAAAT AGCTGGCACT AATAAAGGAA TAACTGCATT ACAGGCTGAT 
ATTAAATTAC CTGGAATACC AATAAAAATT GTGATGGAGG CTATTCAACA AGCTTCAGTG 
GCAAAAAAGG AGATATTACA GATCATGAAC AAAACTATTT CAAAACCTCG AGCATCTAGA 
AAAGAAAATG GACCTGTTGT AGAAACTGTT CAGGTTCCAT TATCAAAACG AGCAAAATTT 
GTTGGACCTG GTGGCTATAA CTTAAAAAAA CTTCAGGCTG AAACAGGTGT AACTATTAGT 
CAGGTGGATG AAGAAACGTT TTCTGTATTT GCACCAACAC CCAGTGCTAT GCATGAGGCA 
AGAGACTTCA TTACTGAAAT CTGCAAGGAT GATCAGGAGC AGCAATTAGA ATTTGGAGCA 
GTATATACCG CCACAATAAC TGAAATCAGA GATACTGGTG TAATGGTAAA ATTATATCCA 
AATATGACTG CGGTACTGCT TCATAACACA CAACTTGATC AACGAAAGAT TAAACATCCT 
ACTGCCCTAG GATTAGAAGT TGGCCAAGAA ATTCAGGTGA AATACTTTGG ACGTGACCCA 
GCCGATGGAA GAATGAGGCT TTCTCGAAAA GTGCTTCAGT CGCCAGCTAC AACCGTGGTC 
AGAACTTTGA ATGACAGAAG TAGTATTGTA ATGGGAGAAC CTATTTCACA GTCATCATCT 
AATTCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of homologous phosphorolytic ribonucleases by citrate may represent an evolutionarily conserved communicative link between RNA degradation and central metabolism.
Nucleic acids research45(8)4655-4666(2017 May)
Stone CM,Butt LE,Bufton JC,Lourenco DC,Gowers DM,Pickford AR,Cox PA,Vincent HA,Callaghan AJ

Whole-exome sequencing identifies novel variants in PNPT1 causing oxidative phosphorylation defects and severe multisystem disease.
European journal of human genetics : EJHG25(1)79-84(2016 01)
Alodaib A,Sobreira N,Gold WA,Riley LG,Van Bergen NJ,Wilson MJ,Bennetts B,Thorburn DR,Boehm C,Christodoulou J

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PNPT1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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