PNPase cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PNPase
Entrez Gene ID	43710
Full Name	Polynucleotide phosphorylase
Synonyms	CG11337,Dmel\CG11337,dmpnpase
General protein information	Preferred Names Polynucleotide phosphorylase Names CG11337 gene product from transcript CG11337-RD CG11337-PA CG11337-PB CG11337-PD CG11337-PE PNPase-PA PNPase-PB PNPase-PD PNPase-PE polynucleotide phosphorylase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100C3-100C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_170550.2	NP_733429.1	polynucleotide phosphorylase, isoform B
NM_143610.3	NP_651867.2	polynucleotide phosphorylase, isoform A
NM_001104520.3	NP_001097990.2	polynucleotide phosphorylase, isoform E
NM_001170312.1	NP_001163783.1	polynucleotide phosphorylase, isoform D

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PNPT1	XP_538497.3
Bos taurus (cattle)	PNPT1	NP_001179126.1
Drosophila melanogaster (fruit fly)	CG11337	NP_001097990.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010791	XP_318517.3
Homo sapiens (human)	PNPT1	NP_149100.2
	PNPT1	XP_001112421.1
Danio rerio (zebrafish)	pnpt1	NP_001238761.1
Gallus gallus (chicken)	PNPT1	XP_419282.4
Caenorhabditis elegans (roundworm)	BE0003N10.1	NP_497324.3
Xenopus tropicalis (tropical clawed frog)	pnpt1	NP_001017033.1
Pan troglodytes (chimpanzee)	PNPT1	XP_515490.2
Mus musculus (house mouse)	Pnpt1	NP_082145.1
Rattus norvegicus (Norway rat)	Pnpt1	NP_001135843.1
Arabidopsis thaliana (thale cress)	AT5G14580	NP_196962.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PNPase gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PNPase cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06758	NM_001104520.3 Latest version!	Drosophila melanogaster polynucleotide phosphorylase, transcript variant E (PNPase), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06758	NM_143610.3 Latest version!	Drosophila melanogaster polynucleotide phosphorylase, transcript variant A (PNPase), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06758	NM_001170312.1 Latest version!	Drosophila melanogaster polynucleotide phosphorylase, transcript variant D (PNPase), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06758	NM_170550.2 Latest version!	Drosophila melanogaster polynucleotide phosphorylase, transcript variant B (PNPase), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PNPase ( NM_170550.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733429.1 Drosophila melanogaster polynucleotide phosphorylase, transcript variant B (PNPase), mRNA.

Price & Availability↑

CloneID	OFa06758
Clone ID Related Accession (Same CDS sequence)	NM_170550.2 , NM_143610.3 , NM_001104520.3 , NM_001170312.1
Accession Version	NM_170550.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	polynucleotide phosphorylase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_170550.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATGA TTTTCACACG GAAATCGCTG AAGCTGCTCA ACTATCGCCT CAAATGCCTA 
AGTTTGTCCT GCCCAGGAGG AAGGAGAGGA ATCCAAAGCA GTTCCAACGG AGAAGCGCCC 
TCCGTGGAAG TCAACTTCAG CAATGGACGT AACATGACCT TCAGTTCCGG CCGCCTGGCG 
AGATTTGCGA ATGGAACAGC TGTGTGCCAA ATGGGCGATA CCGCCGTCAT GGTCACCGCG 
GTGGCCAAGG CGAAGCCAAA TCCCGGCCAG GGATTCATGC CTCTGGTGGT TGACTACCGA 
CTGAAAAACG CCGCCTCTGG CAGAATACCC ATGAACTTTA TGCGCCGCGA ACTGGGCCCC 
TCGGAAAAAG AGATCCTGTC CGCCCGCCTT ATCGACCGTT CGCTACGCCC GCTCTTCCAC 
AAAGATTATC GCACGGAGAC ACAGCTGGTG TGCAACATGT TGGCTATGGA CGCGGTGCAC 
AGTCCGGATG TGCTTGCAAT CAATGCCGCT TCCATGGCGC TATCTCTAAG TGACATTCCC 
TGGAATGGAC CCATTGGAGC TGTGCGCGTT GGCCTCTGCG ACGGCGAGGT GCTGATCAAC 
CCCACGCGCC GGGAGCTGCA GACTTCGCAA CTGGATTTGG TAGTCTCCGC CACCAAACAA 
AACCTCGTCG TTATGTTGGA GGGCAAGGGG AATGTGGTTC TTCAGCAGGA CCTTCTAAAG 
GCTATCAAAC AAGGGACTCG TGAGGCCCAA TTCATTATCC ACGAGATCGA GCGACTGCAG 
AAGGCCTATG GTCGACAGAA GCGCGAAGTG GAAGTGGCTG CCGAAGTAGA TCCCGAACTG 
GGGAAAGCCG TTCGTAGCAT GTGCGAAATG CGGCTGAGAG AGATCTTCCA GGATTCGACG 
CACGACAAGA TGTCTCGTGA CAATGCGGTT AACGAGGTGC GCTCCAACGT TATTGACAAG 
GTGTGGAGTT CGTTTCCGGA TACCGAACCG TCGCTTATTA CCGAGCAGTT CAACCAGACC 
AGTAGAACCA TTTTTCGCGA ACTGATCTTT GAGCGGGGTC TGCGTTGCGA TGGACGAGAT 
TACGACCAGC TGAGGAACAT CTCCTGCCAG GTGGACATGT ACAAGCCTCT CCACGGATCC 
GCCCTATTCC AGCGCGGTCA GACGCAGGTC TTCTGCACGG TTAGCTTGGA CTCGCAGGAA 
AGCGCAATGA AACTTGACTC GTTGGCTGCT TTAGATAGCG GTGGCCTGAA GGCCAAGAAC 
TTTATGCTGC ACTATGAGTT CCCGCCCTAT GCCACGGGTG AAGTGGGTCG GATTGGTCCA 
GTGGGTCGCC GGGAAATGGG CCACGGAGCC TTGGCGGAGA GATCGTTGCT TCCCACCCTG 
CCGAATGATT ATCCCTTTAC CGTAAGACTC ACCTCTGAAG TCCTGGAATC CAATGGGAGC 
AGTAGCATGG CCAGCGTTTG CGGTGGCTCC TTGGCCCTTA TGGACGCCGG CGTTCCAGTG 
AGCGCACCTG CGGCAGGTGT GGCCATTGGC CTGGTCACAA AGTTCGAGAA CGACGACACT 
AAGCACCTGC AGGACTACCG CATTCTCACC GACATCCTGG GCATCGAAGA TTACATGGGC 
GACATGGACA TGAAGGTGGC TGGCACGAGA AAAGGCTTCA CCGCCATCCA GGCGGACCTC 
AAGATCCCGG GAATTCCTCT TAAGGTTGTG ATGGAATCGC TACAAAAGGC CACCGATGCC 
AAATCTAATA TTCTGGACAT CATGAGTGAG GCTATTAGGG AACCTCGAAA ATACCCCAAG 
GAGAGCTGGC CAGTGAGTGA AACCCTAACT GTAGAGCCGC AACAGAGGGC GCAGTTAATT 
GGTCCCAGCG GCCTTCACAT GAAGCGGATT TACTTGGAAA CGGGCACCTC CCTAACAGCA 
GTCGATGAGA CCCATTTCAA TGTTTTTGCT CCTTCGCAAG CAGCCATGGA TGAGGCCAAG 
GAGCTGATCG AGGGCTACAT GGTGAAGGAA CGAGTGCCGG ACCTGGAGTT CGGCGGAATC 
TACACTGCCA AAATCACCGA ATTGCGAGAC ACCGGAGTAA TGGTCATCCT CTATCCCAGT 
ATGCCACCCG CTTTGCTGCA CAACTCGCAG CTGGATCAGC GCAAGATAGC CCATCCCTCT 
GCCCTAAACC TGGAAGTGGG TCAGGAGATC CAGGTCAAGT ACTTTGGCCG CGATCCCGTC 
TCCGGCTTTA TGCGTCTTTC GCGAAAGGTT CTCCAAGGGC CCGCCCTGGG AATCCCCCGT 
TCGCTCAACA AATCTGCCGG AGAGAGCGGA ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733429.1
CDS	230..2545
Translation	MAMIFTRKSLKLLNYRLKCLSLSCPGGRRGIQSSSNGEAPSVEVNFSNGRNMTFSSGRLARFANGTAVCQMGDTAVMVTAVAKAKPNPGQGFMPLVVDYRLKNAASGRIPMNFMRRELGPSEKEILSARLIDRSLRPLFHKDYRTETQLVCNMLAMDAVHSPDVLAINAASMALSLSDIPWNGPIGAVRVGLCDGEVLINPTRRELQTSQLDLVVSATKQNLVVMLEGKGNVVLQQDLLKAIKQGTREAQFIIHEIERLQKAYGRQKREVEVAAEVDPELGKAVRSMCEMRLREIFQDSTHDKMSRDNAVNEVRSNVIDKVWSSFPDTEPSLITEQFNQTSRTIFRELIFERGLRCDGRDYDQLRNISCQVDMYKPLHGSALFQRGQTQVFCTVSLDSQESAMKLDSLAALDSGGLKAKNFMLHYEFPPYATGEVGRIGPVGRREMGHGALAERSLLPTLPNDYPFTVRLTSEVLESNGSSSMASVCGGSLALMDAGVPVSAPAAGVAIGLVTKFENDDTKHLQDYRILTDILGIEDYMGDMDMKVAGTRKGFTAIQADLKIPGIPLKVVMESLQKATDAKSNILDIMSEAIREPRKYPKESWPVSETLTVEPQQRAQLIGPSGLHMKRIYLETGTSLTAVDETHFNVFAPSQAAMDEAKELIEGYMVKERVPDLEFGGIYTAKITELRDTGVMVILYPSMPPALLHNSQLDQRKIAHPSALNLEVGQEIQVKYFGRDPVSGFMRLSRKVLQGPALGIPRSLNKSAGESGT

Target ORF information:

RefSeq Version	NM_170550.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster polynucleotide phosphorylase, transcript variant B (PNPase), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170550.2

ORF Insert Sequence:

ATGGCCATGA TTTTCACACG GAAATCGCTG AAGCTGCTCA ACTATCGCCT CAAATGCCTA 
AGTTTGTCCT GCCCAGGAGG AAGGAGAGGA ATCCAAAGCA GTTCCAACGG AGAAGCGCCC 
TCCGTGGAAG TCAACTTCAG CAATGGACGT AACATGACCT TCAGTTCCGG CCGCCTGGCG 
AGATTTGCGA ATGGAACAGC TGTGTGCCAA ATGGGCGATA CCGCCGTCAT GGTCACCGCG 
GTGGCCAAGG CGAAGCCAAA TCCCGGCCAG GGATTCATGC CTCTGGTGGT TGACTACCGA 
CTGAAAAACG CCGCCTCTGG CAGAATACCC ATGAACTTTA TGCGCCGCGA ACTGGGCCCC 
TCGGAAAAAG AGATCCTGTC CGCCCGCCTT ATCGACCGTT CGCTACGCCC GCTCTTCCAC 
AAAGATTATC GCACGGAGAC ACAGCTGGTG TGCAACATGT TGGCTATGGA CGCGGTGCAC 
AGTCCGGATG TGCTTGCAAT CAATGCCGCT TCCATGGCGC TATCTCTAAG TGACATTCCC 
TGGAATGGAC CCATTGGAGC TGTGCGCGTT GGCCTCTGCG ACGGCGAGGT GCTGATCAAC 
CCCACGCGCC GGGAGCTGCA GACTTCGCAA CTGGATTTGG TAGTCTCCGC CACCAAACAA 
AACCTCGTCG TTATGTTGGA GGGCAAGGGG AATGTGGTTC TTCAGCAGGA CCTTCTAAAG 
GCTATCAAAC AAGGGACTCG TGAGGCCCAA TTCATTATCC ACGAGATCGA GCGACTGCAG 
AAGGCCTATG GTCGACAGAA GCGCGAAGTG GAAGTGGCTG CCGAAGTAGA TCCCGAACTG 
GGGAAAGCCG TTCGTAGCAT GTGCGAAATG CGGCTGAGAG AGATCTTCCA GGATTCGACG 
CACGACAAGA TGTCTCGTGA CAATGCGGTT AACGAGGTGC GCTCCAACGT TATTGACAAG 
GTGTGGAGTT CGTTTCCGGA TACCGAACCG TCGCTTATTA CCGAGCAGTT CAACCAGACC 
AGTAGAACCA TTTTTCGCGA ACTGATCTTT GAGCGGGGTC TGCGTTGCGA TGGACGAGAT 
TACGACCAGC TGAGGAACAT CTCCTGCCAG GTGGACATGT ACAAGCCTCT CCACGGATCC 
GCCCTATTCC AGCGCGGTCA GACGCAGGTC TTCTGCACGG TTAGCTTGGA CTCGCAGGAA 
AGCGCAATGA AACTTGACTC GTTGGCTGCT TTAGATAGCG GTGGCCTGAA GGCCAAGAAC 
TTTATGCTGC ACTATGAGTT CCCGCCCTAT GCCACGGGTG AAGTGGGTCG GATTGGTCCA 
GTGGGTCGCC GGGAAATGGG CCACGGAGCC TTGGCGGAGA GATCGTTGCT TCCCACCCTG 
CCGAATGATT ATCCCTTTAC CGTAAGACTC ACCTCTGAAG TCCTGGAATC CAATGGGAGC 
AGTAGCATGG CCAGCGTTTG CGGTGGCTCC TTGGCCCTTA TGGACGCCGG CGTTCCAGTG 
AGCGCACCTG CGGCAGGTGT GGCCATTGGC CTGGTCACAA AGTTCGAGAA CGACGACACT 
AAGCACCTGC AGGACTACCG CATTCTCACC GACATCCTGG GCATCGAAGA TTACATGGGC 
GACATGGACA TGAAGGTGGC TGGCACGAGA AAAGGCTTCA CCGCCATCCA GGCGGACCTC 
AAGATCCCGG GAATTCCTCT TAAGGTTGTG ATGGAATCGC TACAAAAGGC CACCGATGCC 
AAATCTAATA TTCTGGACAT CATGAGTGAG GCTATTAGGG AACCTCGAAA ATACCCCAAG 
GAGAGCTGGC CAGTGAGTGA AACCCTAACT GTAGAGCCGC AACAGAGGGC GCAGTTAATT 
GGTCCCAGCG GCCTTCACAT GAAGCGGATT TACTTGGAAA CGGGCACCTC CCTAACAGCA 
GTCGATGAGA CCCATTTCAA TGTTTTTGCT CCTTCGCAAG CAGCCATGGA TGAGGCCAAG 
GAGCTGATCG AGGGCTACAT GGTGAAGGAA CGAGTGCCGG ACCTGGAGTT CGGCGGAATC 
TACACTGCCA AAATCACCGA ATTGCGAGAC ACCGGAGTAA TGGTCATCCT CTATCCCAGT 
ATGCCACCCG CTTTGCTGCA CAACTCGCAG CTGGATCAGC GCAAGATAGC CCATCCCTCT 
GCCCTAAACC TGGAAGTGGG TCAGGAGATC CAGGTCAAGT ACTTTGGCCG CGATCCCGTC 
TCCGGCTTTA TGCGTCTTTC GCGAAAGGTT CTCCAAGGGC CCGCCCTGGG AATCCCCCGT 
TCGCTCAACA AATCTGCCGG AGAGAGCGGA ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPase ( NM_143610.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651867.2 Drosophila melanogaster polynucleotide phosphorylase, transcript variant A (PNPase), mRNA.

Price & Availability↑

CloneID	OFa06758
Clone ID Related Accession (Same CDS sequence)	NM_170550.2 , NM_143610.3 , NM_001104520.3 , NM_001170312.1
Accession Version	NM_143610.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	polynucleotide phosphorylase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_143610.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATGA TTTTCACACG GAAATCGCTG AAGCTGCTCA ACTATCGCCT CAAATGCCTA 
AGTTTGTCCT GCCCAGGAGG AAGGAGAGGA ATCCAAAGCA GTTCCAACGG AGAAGCGCCC 
TCCGTGGAAG TCAACTTCAG CAATGGACGT AACATGACCT TCAGTTCCGG CCGCCTGGCG 
AGATTTGCGA ATGGAACAGC TGTGTGCCAA ATGGGCGATA CCGCCGTCAT GGTCACCGCG 
GTGGCCAAGG CGAAGCCAAA TCCCGGCCAG GGATTCATGC CTCTGGTGGT TGACTACCGA 
CTGAAAAACG CCGCCTCTGG CAGAATACCC ATGAACTTTA TGCGCCGCGA ACTGGGCCCC 
TCGGAAAAAG AGATCCTGTC CGCCCGCCTT ATCGACCGTT CGCTACGCCC GCTCTTCCAC 
AAAGATTATC GCACGGAGAC ACAGCTGGTG TGCAACATGT TGGCTATGGA CGCGGTGCAC 
AGTCCGGATG TGCTTGCAAT CAATGCCGCT TCCATGGCGC TATCTCTAAG TGACATTCCC 
TGGAATGGAC CCATTGGAGC TGTGCGCGTT GGCCTCTGCG ACGGCGAGGT GCTGATCAAC 
CCCACGCGCC GGGAGCTGCA GACTTCGCAA CTGGATTTGG TAGTCTCCGC CACCAAACAA 
AACCTCGTCG TTATGTTGGA GGGCAAGGGG AATGTGGTTC TTCAGCAGGA CCTTCTAAAG 
GCTATCAAAC AAGGGACTCG TGAGGCCCAA TTCATTATCC ACGAGATCGA GCGACTGCAG 
AAGGCCTATG GTCGACAGAA GCGCGAAGTG GAAGTGGCTG CCGAAGTAGA TCCCGAACTG 
GGGAAAGCCG TTCGTAGCAT GTGCGAAATG CGGCTGAGAG AGATCTTCCA GGATTCGACG 
CACGACAAGA TGTCTCGTGA CAATGCGGTT AACGAGGTGC GCTCCAACGT TATTGACAAG 
GTGTGGAGTT CGTTTCCGGA TACCGAACCG TCGCTTATTA CCGAGCAGTT CAACCAGACC 
AGTAGAACCA TTTTTCGCGA ACTGATCTTT GAGCGGGGTC TGCGTTGCGA TGGACGAGAT 
TACGACCAGC TGAGGAACAT CTCCTGCCAG GTGGACATGT ACAAGCCTCT CCACGGATCC 
GCCCTATTCC AGCGCGGTCA GACGCAGGTC TTCTGCACGG TTAGCTTGGA CTCGCAGGAA 
AGCGCAATGA AACTTGACTC GTTGGCTGCT TTAGATAGCG GTGGCCTGAA GGCCAAGAAC 
TTTATGCTGC ACTATGAGTT CCCGCCCTAT GCCACGGGTG AAGTGGGTCG GATTGGTCCA 
GTGGGTCGCC GGGAAATGGG CCACGGAGCC TTGGCGGAGA GATCGTTGCT TCCCACCCTG 
CCGAATGATT ATCCCTTTAC CGTAAGACTC ACCTCTGAAG TCCTGGAATC CAATGGGAGC 
AGTAGCATGG CCAGCGTTTG CGGTGGCTCC TTGGCCCTTA TGGACGCCGG CGTTCCAGTG 
AGCGCACCTG CGGCAGGTGT GGCCATTGGC CTGGTCACAA AGTTCGAGAA CGACGACACT 
AAGCACCTGC AGGACTACCG CATTCTCACC GACATCCTGG GCATCGAAGA TTACATGGGC 
GACATGGACA TGAAGGTGGC TGGCACGAGA AAAGGCTTCA CCGCCATCCA GGCGGACCTC 
AAGATCCCGG GAATTCCTCT TAAGGTTGTG ATGGAATCGC TACAAAAGGC CACCGATGCC 
AAATCTAATA TTCTGGACAT CATGAGTGAG GCTATTAGGG AACCTCGAAA ATACCCCAAG 
GAGAGCTGGC CAGTGAGTGA AACCCTAACT GTAGAGCCGC AACAGAGGGC GCAGTTAATT 
GGTCCCAGCG GCCTTCACAT GAAGCGGATT TACTTGGAAA CGGGCACCTC CCTAACAGCA 
GTCGATGAGA CCCATTTCAA TGTTTTTGCT CCTTCGCAAG CAGCCATGGA TGAGGCCAAG 
GAGCTGATCG AGGGCTACAT GGTGAAGGAA CGAGTGCCGG ACCTGGAGTT CGGCGGAATC 
TACACTGCCA AAATCACCGA ATTGCGAGAC ACCGGAGTAA TGGTCATCCT CTATCCCAGT 
ATGCCACCCG CTTTGCTGCA CAACTCGCAG CTGGATCAGC GCAAGATAGC CCATCCCTCT 
GCCCTAAACC TGGAAGTGGG TCAGGAGATC CAGGTCAAGT ACTTTGGCCG CGATCCCGTC 
TCCGGCTTTA TGCGTCTTTC GCGAAAGGTT CTCCAAGGGC CCGCCCTGGG AATCCCCCGT 
TCGCTCAACA AATCTGCCGG AGAGAGCGGA ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651867.2
CDS	136..2451
Translation	MAMIFTRKSLKLLNYRLKCLSLSCPGGRRGIQSSSNGEAPSVEVNFSNGRNMTFSSGRLARFANGTAVCQMGDTAVMVTAVAKAKPNPGQGFMPLVVDYRLKNAASGRIPMNFMRRELGPSEKEILSARLIDRSLRPLFHKDYRTETQLVCNMLAMDAVHSPDVLAINAASMALSLSDIPWNGPIGAVRVGLCDGEVLINPTRRELQTSQLDLVVSATKQNLVVMLEGKGNVVLQQDLLKAIKQGTREAQFIIHEIERLQKAYGRQKREVEVAAEVDPELGKAVRSMCEMRLREIFQDSTHDKMSRDNAVNEVRSNVIDKVWSSFPDTEPSLITEQFNQTSRTIFRELIFERGLRCDGRDYDQLRNISCQVDMYKPLHGSALFQRGQTQVFCTVSLDSQESAMKLDSLAALDSGGLKAKNFMLHYEFPPYATGEVGRIGPVGRREMGHGALAERSLLPTLPNDYPFTVRLTSEVLESNGSSSMASVCGGSLALMDAGVPVSAPAAGVAIGLVTKFENDDTKHLQDYRILTDILGIEDYMGDMDMKVAGTRKGFTAIQADLKIPGIPLKVVMESLQKATDAKSNILDIMSEAIREPRKYPKESWPVSETLTVEPQQRAQLIGPSGLHMKRIYLETGTSLTAVDETHFNVFAPSQAAMDEAKELIEGYMVKERVPDLEFGGIYTAKITELRDTGVMVILYPSMPPALLHNSQLDQRKIAHPSALNLEVGQEIQVKYFGRDPVSGFMRLSRKVLQGPALGIPRSLNKSAGESGT

Target ORF information:

RefSeq Version	NM_143610.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster polynucleotide phosphorylase, transcript variant A (PNPase), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143610.3

ORF Insert Sequence:

ATGGCCATGA TTTTCACACG GAAATCGCTG AAGCTGCTCA ACTATCGCCT CAAATGCCTA 
AGTTTGTCCT GCCCAGGAGG AAGGAGAGGA ATCCAAAGCA GTTCCAACGG AGAAGCGCCC 
TCCGTGGAAG TCAACTTCAG CAATGGACGT AACATGACCT TCAGTTCCGG CCGCCTGGCG 
AGATTTGCGA ATGGAACAGC TGTGTGCCAA ATGGGCGATA CCGCCGTCAT GGTCACCGCG 
GTGGCCAAGG CGAAGCCAAA TCCCGGCCAG GGATTCATGC CTCTGGTGGT TGACTACCGA 
CTGAAAAACG CCGCCTCTGG CAGAATACCC ATGAACTTTA TGCGCCGCGA ACTGGGCCCC 
TCGGAAAAAG AGATCCTGTC CGCCCGCCTT ATCGACCGTT CGCTACGCCC GCTCTTCCAC 
AAAGATTATC GCACGGAGAC ACAGCTGGTG TGCAACATGT TGGCTATGGA CGCGGTGCAC 
AGTCCGGATG TGCTTGCAAT CAATGCCGCT TCCATGGCGC TATCTCTAAG TGACATTCCC 
TGGAATGGAC CCATTGGAGC TGTGCGCGTT GGCCTCTGCG ACGGCGAGGT GCTGATCAAC 
CCCACGCGCC GGGAGCTGCA GACTTCGCAA CTGGATTTGG TAGTCTCCGC CACCAAACAA 
AACCTCGTCG TTATGTTGGA GGGCAAGGGG AATGTGGTTC TTCAGCAGGA CCTTCTAAAG 
GCTATCAAAC AAGGGACTCG TGAGGCCCAA TTCATTATCC ACGAGATCGA GCGACTGCAG 
AAGGCCTATG GTCGACAGAA GCGCGAAGTG GAAGTGGCTG CCGAAGTAGA TCCCGAACTG 
GGGAAAGCCG TTCGTAGCAT GTGCGAAATG CGGCTGAGAG AGATCTTCCA GGATTCGACG 
CACGACAAGA TGTCTCGTGA CAATGCGGTT AACGAGGTGC GCTCCAACGT TATTGACAAG 
GTGTGGAGTT CGTTTCCGGA TACCGAACCG TCGCTTATTA CCGAGCAGTT CAACCAGACC 
AGTAGAACCA TTTTTCGCGA ACTGATCTTT GAGCGGGGTC TGCGTTGCGA TGGACGAGAT 
TACGACCAGC TGAGGAACAT CTCCTGCCAG GTGGACATGT ACAAGCCTCT CCACGGATCC 
GCCCTATTCC AGCGCGGTCA GACGCAGGTC TTCTGCACGG TTAGCTTGGA CTCGCAGGAA 
AGCGCAATGA AACTTGACTC GTTGGCTGCT TTAGATAGCG GTGGCCTGAA GGCCAAGAAC 
TTTATGCTGC ACTATGAGTT CCCGCCCTAT GCCACGGGTG AAGTGGGTCG GATTGGTCCA 
GTGGGTCGCC GGGAAATGGG CCACGGAGCC TTGGCGGAGA GATCGTTGCT TCCCACCCTG 
CCGAATGATT ATCCCTTTAC CGTAAGACTC ACCTCTGAAG TCCTGGAATC CAATGGGAGC 
AGTAGCATGG CCAGCGTTTG CGGTGGCTCC TTGGCCCTTA TGGACGCCGG CGTTCCAGTG 
AGCGCACCTG CGGCAGGTGT GGCCATTGGC CTGGTCACAA AGTTCGAGAA CGACGACACT 
AAGCACCTGC AGGACTACCG CATTCTCACC GACATCCTGG GCATCGAAGA TTACATGGGC 
GACATGGACA TGAAGGTGGC TGGCACGAGA AAAGGCTTCA CCGCCATCCA GGCGGACCTC 
AAGATCCCGG GAATTCCTCT TAAGGTTGTG ATGGAATCGC TACAAAAGGC CACCGATGCC 
AAATCTAATA TTCTGGACAT CATGAGTGAG GCTATTAGGG AACCTCGAAA ATACCCCAAG 
GAGAGCTGGC CAGTGAGTGA AACCCTAACT GTAGAGCCGC AACAGAGGGC GCAGTTAATT 
GGTCCCAGCG GCCTTCACAT GAAGCGGATT TACTTGGAAA CGGGCACCTC CCTAACAGCA 
GTCGATGAGA CCCATTTCAA TGTTTTTGCT CCTTCGCAAG CAGCCATGGA TGAGGCCAAG 
GAGCTGATCG AGGGCTACAT GGTGAAGGAA CGAGTGCCGG ACCTGGAGTT CGGCGGAATC 
TACACTGCCA AAATCACCGA ATTGCGAGAC ACCGGAGTAA TGGTCATCCT CTATCCCAGT 
ATGCCACCCG CTTTGCTGCA CAACTCGCAG CTGGATCAGC GCAAGATAGC CCATCCCTCT 
GCCCTAAACC TGGAAGTGGG TCAGGAGATC CAGGTCAAGT ACTTTGGCCG CGATCCCGTC 
TCCGGCTTTA TGCGTCTTTC GCGAAAGGTT CTCCAAGGGC CCGCCCTGGG AATCCCCCGT 
TCGCTCAACA AATCTGCCGG AGAGAGCGGA ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPase ( NM_001104520.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097990.2 Drosophila melanogaster polynucleotide phosphorylase, transcript variant E (PNPase), mRNA.

Price & Availability↑

CloneID	OFa06758
Clone ID Related Accession (Same CDS sequence)	NM_170550.2 , NM_143610.3 , NM_001104520.3 , NM_001170312.1
Accession Version	NM_001104520.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	polynucleotide phosphorylase, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001104520.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATGA TTTTCACACG GAAATCGCTG AAGCTGCTCA ACTATCGCCT CAAATGCCTA 
AGTTTGTCCT GCCCAGGAGG AAGGAGAGGA ATCCAAAGCA GTTCCAACGG AGAAGCGCCC 
TCCGTGGAAG TCAACTTCAG CAATGGACGT AACATGACCT TCAGTTCCGG CCGCCTGGCG 
AGATTTGCGA ATGGAACAGC TGTGTGCCAA ATGGGCGATA CCGCCGTCAT GGTCACCGCG 
GTGGCCAAGG CGAAGCCAAA TCCCGGCCAG GGATTCATGC CTCTGGTGGT TGACTACCGA 
CTGAAAAACG CCGCCTCTGG CAGAATACCC ATGAACTTTA TGCGCCGCGA ACTGGGCCCC 
TCGGAAAAAG AGATCCTGTC CGCCCGCCTT ATCGACCGTT CGCTACGCCC GCTCTTCCAC 
AAAGATTATC GCACGGAGAC ACAGCTGGTG TGCAACATGT TGGCTATGGA CGCGGTGCAC 
AGTCCGGATG TGCTTGCAAT CAATGCCGCT TCCATGGCGC TATCTCTAAG TGACATTCCC 
TGGAATGGAC CCATTGGAGC TGTGCGCGTT GGCCTCTGCG ACGGCGAGGT GCTGATCAAC 
CCCACGCGCC GGGAGCTGCA GACTTCGCAA CTGGATTTGG TAGTCTCCGC CACCAAACAA 
AACCTCGTCG TTATGTTGGA GGGCAAGGGG AATGTGGTTC TTCAGCAGGA CCTTCTAAAG 
GCTATCAAAC AAGGGACTCG TGAGGCCCAA TTCATTATCC ACGAGATCGA GCGACTGCAG 
AAGGCCTATG GTCGACAGAA GCGCGAAGTG GAAGTGGCTG CCGAAGTAGA TCCCGAACTG 
GGGAAAGCCG TTCGTAGCAT GTGCGAAATG CGGCTGAGAG AGATCTTCCA GGATTCGACG 
CACGACAAGA TGTCTCGTGA CAATGCGGTT AACGAGGTGC GCTCCAACGT TATTGACAAG 
GTGTGGAGTT CGTTTCCGGA TACCGAACCG TCGCTTATTA CCGAGCAGTT CAACCAGACC 
AGTAGAACCA TTTTTCGCGA ACTGATCTTT GAGCGGGGTC TGCGTTGCGA TGGACGAGAT 
TACGACCAGC TGAGGAACAT CTCCTGCCAG GTGGACATGT ACAAGCCTCT CCACGGATCC 
GCCCTATTCC AGCGCGGTCA GACGCAGGTC TTCTGCACGG TTAGCTTGGA CTCGCAGGAA 
AGCGCAATGA AACTTGACTC GTTGGCTGCT TTAGATAGCG GTGGCCTGAA GGCCAAGAAC 
TTTATGCTGC ACTATGAGTT CCCGCCCTAT GCCACGGGTG AAGTGGGTCG GATTGGTCCA 
GTGGGTCGCC GGGAAATGGG CCACGGAGCC TTGGCGGAGA GATCGTTGCT TCCCACCCTG 
CCGAATGATT ATCCCTTTAC CGTAAGACTC ACCTCTGAAG TCCTGGAATC CAATGGGAGC 
AGTAGCATGG CCAGCGTTTG CGGTGGCTCC TTGGCCCTTA TGGACGCCGG CGTTCCAGTG 
AGCGCACCTG CGGCAGGTGT GGCCATTGGC CTGGTCACAA AGTTCGAGAA CGACGACACT 
AAGCACCTGC AGGACTACCG CATTCTCACC GACATCCTGG GCATCGAAGA TTACATGGGC 
GACATGGACA TGAAGGTGGC TGGCACGAGA AAAGGCTTCA CCGCCATCCA GGCGGACCTC 
AAGATCCCGG GAATTCCTCT TAAGGTTGTG ATGGAATCGC TACAAAAGGC CACCGATGCC 
AAATCTAATA TTCTGGACAT CATGAGTGAG GCTATTAGGG AACCTCGAAA ATACCCCAAG 
GAGAGCTGGC CAGTGAGTGA AACCCTAACT GTAGAGCCGC AACAGAGGGC GCAGTTAATT 
GGTCCCAGCG GCCTTCACAT GAAGCGGATT TACTTGGAAA CGGGCACCTC CCTAACAGCA 
GTCGATGAGA CCCATTTCAA TGTTTTTGCT CCTTCGCAAG CAGCCATGGA TGAGGCCAAG 
GAGCTGATCG AGGGCTACAT GGTGAAGGAA CGAGTGCCGG ACCTGGAGTT CGGCGGAATC 
TACACTGCCA AAATCACCGA ATTGCGAGAC ACCGGAGTAA TGGTCATCCT CTATCCCAGT 
ATGCCACCCG CTTTGCTGCA CAACTCGCAG CTGGATCAGC GCAAGATAGC CCATCCCTCT 
GCCCTAAACC TGGAAGTGGG TCAGGAGATC CAGGTCAAGT ACTTTGGCCG CGATCCCGTC 
TCCGGCTTTA TGCGTCTTTC GCGAAAGGTT CTCCAAGGGC CCGCCCTGGG AATCCCCCGT 
TCGCTCAACA AATCTGCCGG AGAGAGCGGA ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097990.2
CDS	115..2430
Translation	MAMIFTRKSLKLLNYRLKCLSLSCPGGRRGIQSSSNGEAPSVEVNFSNGRNMTFSSGRLARFANGTAVCQMGDTAVMVTAVAKAKPNPGQGFMPLVVDYRLKNAASGRIPMNFMRRELGPSEKEILSARLIDRSLRPLFHKDYRTETQLVCNMLAMDAVHSPDVLAINAASMALSLSDIPWNGPIGAVRVGLCDGEVLINPTRRELQTSQLDLVVSATKQNLVVMLEGKGNVVLQQDLLKAIKQGTREAQFIIHEIERLQKAYGRQKREVEVAAEVDPELGKAVRSMCEMRLREIFQDSTHDKMSRDNAVNEVRSNVIDKVWSSFPDTEPSLITEQFNQTSRTIFRELIFERGLRCDGRDYDQLRNISCQVDMYKPLHGSALFQRGQTQVFCTVSLDSQESAMKLDSLAALDSGGLKAKNFMLHYEFPPYATGEVGRIGPVGRREMGHGALAERSLLPTLPNDYPFTVRLTSEVLESNGSSSMASVCGGSLALMDAGVPVSAPAAGVAIGLVTKFENDDTKHLQDYRILTDILGIEDYMGDMDMKVAGTRKGFTAIQADLKIPGIPLKVVMESLQKATDAKSNILDIMSEAIREPRKYPKESWPVSETLTVEPQQRAQLIGPSGLHMKRIYLETGTSLTAVDETHFNVFAPSQAAMDEAKELIEGYMVKERVPDLEFGGIYTAKITELRDTGVMVILYPSMPPALLHNSQLDQRKIAHPSALNLEVGQEIQVKYFGRDPVSGFMRLSRKVLQGPALGIPRSLNKSAGESGT

Target ORF information:

RefSeq Version	NM_001104520.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster polynucleotide phosphorylase, transcript variant E (PNPase), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104520.3

ORF Insert Sequence:

ATGGCCATGA TTTTCACACG GAAATCGCTG AAGCTGCTCA ACTATCGCCT CAAATGCCTA 
AGTTTGTCCT GCCCAGGAGG AAGGAGAGGA ATCCAAAGCA GTTCCAACGG AGAAGCGCCC 
TCCGTGGAAG TCAACTTCAG CAATGGACGT AACATGACCT TCAGTTCCGG CCGCCTGGCG 
AGATTTGCGA ATGGAACAGC TGTGTGCCAA ATGGGCGATA CCGCCGTCAT GGTCACCGCG 
GTGGCCAAGG CGAAGCCAAA TCCCGGCCAG GGATTCATGC CTCTGGTGGT TGACTACCGA 
CTGAAAAACG CCGCCTCTGG CAGAATACCC ATGAACTTTA TGCGCCGCGA ACTGGGCCCC 
TCGGAAAAAG AGATCCTGTC CGCCCGCCTT ATCGACCGTT CGCTACGCCC GCTCTTCCAC 
AAAGATTATC GCACGGAGAC ACAGCTGGTG TGCAACATGT TGGCTATGGA CGCGGTGCAC 
AGTCCGGATG TGCTTGCAAT CAATGCCGCT TCCATGGCGC TATCTCTAAG TGACATTCCC 
TGGAATGGAC CCATTGGAGC TGTGCGCGTT GGCCTCTGCG ACGGCGAGGT GCTGATCAAC 
CCCACGCGCC GGGAGCTGCA GACTTCGCAA CTGGATTTGG TAGTCTCCGC CACCAAACAA 
AACCTCGTCG TTATGTTGGA GGGCAAGGGG AATGTGGTTC TTCAGCAGGA CCTTCTAAAG 
GCTATCAAAC AAGGGACTCG TGAGGCCCAA TTCATTATCC ACGAGATCGA GCGACTGCAG 
AAGGCCTATG GTCGACAGAA GCGCGAAGTG GAAGTGGCTG CCGAAGTAGA TCCCGAACTG 
GGGAAAGCCG TTCGTAGCAT GTGCGAAATG CGGCTGAGAG AGATCTTCCA GGATTCGACG 
CACGACAAGA TGTCTCGTGA CAATGCGGTT AACGAGGTGC GCTCCAACGT TATTGACAAG 
GTGTGGAGTT CGTTTCCGGA TACCGAACCG TCGCTTATTA CCGAGCAGTT CAACCAGACC 
AGTAGAACCA TTTTTCGCGA ACTGATCTTT GAGCGGGGTC TGCGTTGCGA TGGACGAGAT 
TACGACCAGC TGAGGAACAT CTCCTGCCAG GTGGACATGT ACAAGCCTCT CCACGGATCC 
GCCCTATTCC AGCGCGGTCA GACGCAGGTC TTCTGCACGG TTAGCTTGGA CTCGCAGGAA 
AGCGCAATGA AACTTGACTC GTTGGCTGCT TTAGATAGCG GTGGCCTGAA GGCCAAGAAC 
TTTATGCTGC ACTATGAGTT CCCGCCCTAT GCCACGGGTG AAGTGGGTCG GATTGGTCCA 
GTGGGTCGCC GGGAAATGGG CCACGGAGCC TTGGCGGAGA GATCGTTGCT TCCCACCCTG 
CCGAATGATT ATCCCTTTAC CGTAAGACTC ACCTCTGAAG TCCTGGAATC CAATGGGAGC 
AGTAGCATGG CCAGCGTTTG CGGTGGCTCC TTGGCCCTTA TGGACGCCGG CGTTCCAGTG 
AGCGCACCTG CGGCAGGTGT GGCCATTGGC CTGGTCACAA AGTTCGAGAA CGACGACACT 
AAGCACCTGC AGGACTACCG CATTCTCACC GACATCCTGG GCATCGAAGA TTACATGGGC 
GACATGGACA TGAAGGTGGC TGGCACGAGA AAAGGCTTCA CCGCCATCCA GGCGGACCTC 
AAGATCCCGG GAATTCCTCT TAAGGTTGTG ATGGAATCGC TACAAAAGGC CACCGATGCC 
AAATCTAATA TTCTGGACAT CATGAGTGAG GCTATTAGGG AACCTCGAAA ATACCCCAAG 
GAGAGCTGGC CAGTGAGTGA AACCCTAACT GTAGAGCCGC AACAGAGGGC GCAGTTAATT 
GGTCCCAGCG GCCTTCACAT GAAGCGGATT TACTTGGAAA CGGGCACCTC CCTAACAGCA 
GTCGATGAGA CCCATTTCAA TGTTTTTGCT CCTTCGCAAG CAGCCATGGA TGAGGCCAAG 
GAGCTGATCG AGGGCTACAT GGTGAAGGAA CGAGTGCCGG ACCTGGAGTT CGGCGGAATC 
TACACTGCCA AAATCACCGA ATTGCGAGAC ACCGGAGTAA TGGTCATCCT CTATCCCAGT 
ATGCCACCCG CTTTGCTGCA CAACTCGCAG CTGGATCAGC GCAAGATAGC CCATCCCTCT 
GCCCTAAACC TGGAAGTGGG TCAGGAGATC CAGGTCAAGT ACTTTGGCCG CGATCCCGTC 
TCCGGCTTTA TGCGTCTTTC GCGAAAGGTT CTCCAAGGGC CCGCCCTGGG AATCCCCCGT 
TCGCTCAACA AATCTGCCGG AGAGAGCGGA ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPase ( NM_001170312.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163783.1 Drosophila melanogaster polynucleotide phosphorylase, transcript variant D (PNPase), mRNA.

Price & Availability↑

CloneID	OFa06758
Clone ID Related Accession (Same CDS sequence)	NM_170550.2 , NM_143610.3 , NM_001104520.3 , NM_001170312.1
Accession Version	NM_001170312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	polynucleotide phosphorylase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATGA TTTTCACACG GAAATCGCTG AAGCTGCTCA ACTATCGCCT CAAATGCCTA 
AGTTTGTCCT GCCCAGGAGG AAGGAGAGGA ATCCAAAGCA GTTCCAACGG AGAAGCGCCC 
TCCGTGGAAG TCAACTTCAG CAATGGACGT AACATGACCT TCAGTTCCGG CCGCCTGGCG 
AGATTTGCGA ATGGAACAGC TGTGTGCCAA ATGGGCGATA CCGCCGTCAT GGTCACCGCG 
GTGGCCAAGG CGAAGCCAAA TCCCGGCCAG GGATTCATGC CTCTGGTGGT TGACTACCGA 
CTGAAAAACG CCGCCTCTGG CAGAATACCC ATGAACTTTA TGCGCCGCGA ACTGGGCCCC 
TCGGAAAAAG AGATCCTGTC CGCCCGCCTT ATCGACCGTT CGCTACGCCC GCTCTTCCAC 
AAAGATTATC GCACGGAGAC ACAGCTGGTG TGCAACATGT TGGCTATGGA CGCGGTGCAC 
AGTCCGGATG TGCTTGCAAT CAATGCCGCT TCCATGGCGC TATCTCTAAG TGACATTCCC 
TGGAATGGAC CCATTGGAGC TGTGCGCGTT GGCCTCTGCG ACGGCGAGGT GCTGATCAAC 
CCCACGCGCC GGGAGCTGCA GACTTCGCAA CTGGATTTGG TAGTCTCCGC CACCAAACAA 
AACCTCGTCG TTATGTTGGA GGGCAAGGGG AATGTGGTTC TTCAGCAGGA CCTTCTAAAG 
GCTATCAAAC AAGGGACTCG TGAGGCCCAA TTCATTATCC ACGAGATCGA GCGACTGCAG 
AAGGCCTATG GTCGACAGAA GCGCGAAGTG GAAGTGGCTG CCGAAGTAGA TCCCGAACTG 
GGGAAAGCCG TTCGTAGCAT GTGCGAAATG CGGCTGAGAG AGATCTTCCA GGATTCGACG 
CACGACAAGA TGTCTCGTGA CAATGCGGTT AACGAGGTGC GCTCCAACGT TATTGACAAG 
GTGTGGAGTT CGTTTCCGGA TACCGAACCG TCGCTTATTA CCGAGCAGTT CAACCAGACC 
AGTAGAACCA TTTTTCGCGA ACTGATCTTT GAGCGGGGTC TGCGTTGCGA TGGACGAGAT 
TACGACCAGC TGAGGAACAT CTCCTGCCAG GTGGACATGT ACAAGCCTCT CCACGGATCC 
GCCCTATTCC AGCGCGGTCA GACGCAGGTC TTCTGCACGG TTAGCTTGGA CTCGCAGGAA 
AGCGCAATGA AACTTGACTC GTTGGCTGCT TTAGATAGCG GTGGCCTGAA GGCCAAGAAC 
TTTATGCTGC ACTATGAGTT CCCGCCCTAT GCCACGGGTG AAGTGGGTCG GATTGGTCCA 
GTGGGTCGCC GGGAAATGGG CCACGGAGCC TTGGCGGAGA GATCGTTGCT TCCCACCCTG 
CCGAATGATT ATCCCTTTAC CGTAAGACTC ACCTCTGAAG TCCTGGAATC CAATGGGAGC 
AGTAGCATGG CCAGCGTTTG CGGTGGCTCC TTGGCCCTTA TGGACGCCGG CGTTCCAGTG 
AGCGCACCTG CGGCAGGTGT GGCCATTGGC CTGGTCACAA AGTTCGAGAA CGACGACACT 
AAGCACCTGC AGGACTACCG CATTCTCACC GACATCCTGG GCATCGAAGA TTACATGGGC 
GACATGGACA TGAAGGTGGC TGGCACGAGA AAAGGCTTCA CCGCCATCCA GGCGGACCTC 
AAGATCCCGG GAATTCCTCT TAAGGTTGTG ATGGAATCGC TACAAAAGGC CACCGATGCC 
AAATCTAATA TTCTGGACAT CATGAGTGAG GCTATTAGGG AACCTCGAAA ATACCCCAAG 
GAGAGCTGGC CAGTGAGTGA AACCCTAACT GTAGAGCCGC AACAGAGGGC GCAGTTAATT 
GGTCCCAGCG GCCTTCACAT GAAGCGGATT TACTTGGAAA CGGGCACCTC CCTAACAGCA 
GTCGATGAGA CCCATTTCAA TGTTTTTGCT CCTTCGCAAG CAGCCATGGA TGAGGCCAAG 
GAGCTGATCG AGGGCTACAT GGTGAAGGAA CGAGTGCCGG ACCTGGAGTT CGGCGGAATC 
TACACTGCCA AAATCACCGA ATTGCGAGAC ACCGGAGTAA TGGTCATCCT CTATCCCAGT 
ATGCCACCCG CTTTGCTGCA CAACTCGCAG CTGGATCAGC GCAAGATAGC CCATCCCTCT 
GCCCTAAACC TGGAAGTGGG TCAGGAGATC CAGGTCAAGT ACTTTGGCCG CGATCCCGTC 
TCCGGCTTTA TGCGTCTTTC GCGAAAGGTT CTCCAAGGGC CCGCCCTGGG AATCCCCCGT 
TCGCTCAACA AATCTGCCGG AGAGAGCGGA ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163783.1
CDS	12..2327
Translation	MAMIFTRKSLKLLNYRLKCLSLSCPGGRRGIQSSSNGEAPSVEVNFSNGRNMTFSSGRLARFANGTAVCQMGDTAVMVTAVAKAKPNPGQGFMPLVVDYRLKNAASGRIPMNFMRRELGPSEKEILSARLIDRSLRPLFHKDYRTETQLVCNMLAMDAVHSPDVLAINAASMALSLSDIPWNGPIGAVRVGLCDGEVLINPTRRELQTSQLDLVVSATKQNLVVMLEGKGNVVLQQDLLKAIKQGTREAQFIIHEIERLQKAYGRQKREVEVAAEVDPELGKAVRSMCEMRLREIFQDSTHDKMSRDNAVNEVRSNVIDKVWSSFPDTEPSLITEQFNQTSRTIFRELIFERGLRCDGRDYDQLRNISCQVDMYKPLHGSALFQRGQTQVFCTVSLDSQESAMKLDSLAALDSGGLKAKNFMLHYEFPPYATGEVGRIGPVGRREMGHGALAERSLLPTLPNDYPFTVRLTSEVLESNGSSSMASVCGGSLALMDAGVPVSAPAAGVAIGLVTKFENDDTKHLQDYRILTDILGIEDYMGDMDMKVAGTRKGFTAIQADLKIPGIPLKVVMESLQKATDAKSNILDIMSEAIREPRKYPKESWPVSETLTVEPQQRAQLIGPSGLHMKRIYLETGTSLTAVDETHFNVFAPSQAAMDEAKELIEGYMVKERVPDLEFGGIYTAKITELRDTGVMVILYPSMPPALLHNSQLDQRKIAHPSALNLEVGQEIQVKYFGRDPVSGFMRLSRKVLQGPALGIPRSLNKSAGESGT

Target ORF information:

RefSeq Version	NM_001170312.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster polynucleotide phosphorylase, transcript variant D (PNPase), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170312.1

ORF Insert Sequence:

ATGGCCATGA TTTTCACACG GAAATCGCTG AAGCTGCTCA ACTATCGCCT CAAATGCCTA 
AGTTTGTCCT GCCCAGGAGG AAGGAGAGGA ATCCAAAGCA GTTCCAACGG AGAAGCGCCC 
TCCGTGGAAG TCAACTTCAG CAATGGACGT AACATGACCT TCAGTTCCGG CCGCCTGGCG 
AGATTTGCGA ATGGAACAGC TGTGTGCCAA ATGGGCGATA CCGCCGTCAT GGTCACCGCG 
GTGGCCAAGG CGAAGCCAAA TCCCGGCCAG GGATTCATGC CTCTGGTGGT TGACTACCGA 
CTGAAAAACG CCGCCTCTGG CAGAATACCC ATGAACTTTA TGCGCCGCGA ACTGGGCCCC 
TCGGAAAAAG AGATCCTGTC CGCCCGCCTT ATCGACCGTT CGCTACGCCC GCTCTTCCAC 
AAAGATTATC GCACGGAGAC ACAGCTGGTG TGCAACATGT TGGCTATGGA CGCGGTGCAC 
AGTCCGGATG TGCTTGCAAT CAATGCCGCT TCCATGGCGC TATCTCTAAG TGACATTCCC 
TGGAATGGAC CCATTGGAGC TGTGCGCGTT GGCCTCTGCG ACGGCGAGGT GCTGATCAAC 
CCCACGCGCC GGGAGCTGCA GACTTCGCAA CTGGATTTGG TAGTCTCCGC CACCAAACAA 
AACCTCGTCG TTATGTTGGA GGGCAAGGGG AATGTGGTTC TTCAGCAGGA CCTTCTAAAG 
GCTATCAAAC AAGGGACTCG TGAGGCCCAA TTCATTATCC ACGAGATCGA GCGACTGCAG 
AAGGCCTATG GTCGACAGAA GCGCGAAGTG GAAGTGGCTG CCGAAGTAGA TCCCGAACTG 
GGGAAAGCCG TTCGTAGCAT GTGCGAAATG CGGCTGAGAG AGATCTTCCA GGATTCGACG 
CACGACAAGA TGTCTCGTGA CAATGCGGTT AACGAGGTGC GCTCCAACGT TATTGACAAG 
GTGTGGAGTT CGTTTCCGGA TACCGAACCG TCGCTTATTA CCGAGCAGTT CAACCAGACC 
AGTAGAACCA TTTTTCGCGA ACTGATCTTT GAGCGGGGTC TGCGTTGCGA TGGACGAGAT 
TACGACCAGC TGAGGAACAT CTCCTGCCAG GTGGACATGT ACAAGCCTCT CCACGGATCC 
GCCCTATTCC AGCGCGGTCA GACGCAGGTC TTCTGCACGG TTAGCTTGGA CTCGCAGGAA 
AGCGCAATGA AACTTGACTC GTTGGCTGCT TTAGATAGCG GTGGCCTGAA GGCCAAGAAC 
TTTATGCTGC ACTATGAGTT CCCGCCCTAT GCCACGGGTG AAGTGGGTCG GATTGGTCCA 
GTGGGTCGCC GGGAAATGGG CCACGGAGCC TTGGCGGAGA GATCGTTGCT TCCCACCCTG 
CCGAATGATT ATCCCTTTAC CGTAAGACTC ACCTCTGAAG TCCTGGAATC CAATGGGAGC 
AGTAGCATGG CCAGCGTTTG CGGTGGCTCC TTGGCCCTTA TGGACGCCGG CGTTCCAGTG 
AGCGCACCTG CGGCAGGTGT GGCCATTGGC CTGGTCACAA AGTTCGAGAA CGACGACACT 
AAGCACCTGC AGGACTACCG CATTCTCACC GACATCCTGG GCATCGAAGA TTACATGGGC 
GACATGGACA TGAAGGTGGC TGGCACGAGA AAAGGCTTCA CCGCCATCCA GGCGGACCTC 
AAGATCCCGG GAATTCCTCT TAAGGTTGTG ATGGAATCGC TACAAAAGGC CACCGATGCC 
AAATCTAATA TTCTGGACAT CATGAGTGAG GCTATTAGGG AACCTCGAAA ATACCCCAAG 
GAGAGCTGGC CAGTGAGTGA AACCCTAACT GTAGAGCCGC AACAGAGGGC GCAGTTAATT 
GGTCCCAGCG GCCTTCACAT GAAGCGGATT TACTTGGAAA CGGGCACCTC CCTAACAGCA 
GTCGATGAGA CCCATTTCAA TGTTTTTGCT CCTTCGCAAG CAGCCATGGA TGAGGCCAAG 
GAGCTGATCG AGGGCTACAT GGTGAAGGAA CGAGTGCCGG ACCTGGAGTT CGGCGGAATC 
TACACTGCCA AAATCACCGA ATTGCGAGAC ACCGGAGTAA TGGTCATCCT CTATCCCAGT 
ATGCCACCCG CTTTGCTGCA CAACTCGCAG CTGGATCAGC GCAAGATAGC CCATCCCTCT 
GCCCTAAACC TGGAAGTGGG TCAGGAGATC CAGGTCAAGT ACTTTGGCCG CGATCCCGTC 
TCCGGCTTTA TGCGTCTTTC GCGAAAGGTT CTCCAAGGGC CCGCCCTGGG AATCCCCCGT 
TCGCTCAACA AATCTGCCGG AGAGAGCGGA ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PNPase cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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