Pnpt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pnpt1
Entrez Gene ID	360992
Full Name	polyribonucleotide nucleotidyltransferase 1
General protein information	Preferred Names polyribonucleotide nucleotidyltransferase 1 Names polyribonucleotide nucleotidyltransferase 1, mitochondrial
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001142371.1	NP_001135843.1	polyribonucleotide nucleotidyltransferase 1, mitochondrial

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PNPT1	XP_538497.3
Bos taurus (cattle)	PNPT1	NP_001179126.1
Drosophila melanogaster (fruit fly)	CG11337	NP_001097990.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010791	XP_318517.3
Homo sapiens (human)	PNPT1	NP_149100.2
	PNPT1	XP_001112421.1
Danio rerio (zebrafish)	pnpt1	NP_001238761.1
Gallus gallus (chicken)	PNPT1	XP_419282.4
Caenorhabditis elegans (roundworm)	BE0003N10.1	NP_497324.3
Xenopus tropicalis (tropical clawed frog)	pnpt1	NP_001017033.1
Pan troglodytes (chimpanzee)	PNPT1	XP_515490.2
Mus musculus (house mouse)	Pnpt1	NP_082145.1
Rattus norvegicus (Norway rat)	Pnpt1	NP_001135843.1
Arabidopsis thaliana (thale cress)	AT5G14580	NP_196962.1

Gene Ontology
Bibliography

Related articles in PubMed

A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss.
von Ameln S, Wang G, Boulouiz R, Rutherford MA, Smith GM, Li Y, Pogoda HM, N?rnberg G, Stiller B, Volk AE, Borck G, Hong JS, Goodyear RJ, Abidi O, N?rnberg P, Hofmann K, Richardson GP, Hammerschmidt M, Moser T, Wollnik B, Koehler CM, Teitell MA, Barakat A, Kubisch C
American journal of human genetics91(5)919-27(2012 Nov)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

PNPASE regulates RNA import into mitochondria.
Wang G, Chen HW, Oktay Y, Zhang J, Allen EL, Smith GM, Fan KC, Hong JS, French SW, McCaffery JM, Lightowlers RN, Morse HC 3rd, Koehler CM, Teitell MA
Cell142(3)456-67(2010 Aug)

Human polynucleotide phosphorylase selectively and preferentially degrades microRNA-221 in human melanoma cells.
Das SK, Sokhi UK, Bhutia SK, Azab B, Su ZZ, Sarkar D, Fisher PB
Proceedings of the National Academy of Sciences of the United States of America107(26)11948-53(2010 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pnpt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pnpt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04321	NM_001142371.1 Latest version!	Rattus norvegicus polyribonucleotide nucleotidyltransferase 1 (Pnpt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pnpt1 ( NM_001142371.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001135843.1 Rattus norvegicus polyribonucleotide nucleotidyltransferase 1 (Pnpt1), mRNA.

Price & Availability↑

CloneID	ORa04321
Clone ID Related Accession (Same CDS sequence)	NM_001142371.1
Accession Version	NM_001142371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	polyribonucleotide nucleotidyltransferase 1, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07016754.1. On Nov 29, 2008 this sequence version replaced XM_341266.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCTT GCAGGCCGTG CTGCTTGTGC CTGTGTCTCC GACCGCTGAG CTGCGGTCCC 
CTCGGCCGGC CAAGGCGGCA TCGTGCACTC AGCTATTTGC AGGTGCGAGC GCTGTGGAGC 
GGCACCCGGT CCAGAGCAGT GACTGTGGAC TTGGGACACA GAAAATTAGA AATATCCTCT 
GGAAAACTGG CAAGATTTGC TGATGGCTGT GCTGTGGTAC AGTCAGGTGA TACTGCAGTA 
ATGGTCACAG CGGTCAGCAA AACAAAACCT TCACCTTCCC AATTCATGCC ATTGGTGGTA 
GACTACAGAC AGAAGGCTGC TGCAGCAGGT AGAATCCCCA CAAACTACCT TAGAAGAGAG 
ATTGGCTCTT CTGACAAAGA GATTCTTACA AGTCGAGTAA TAGATCGTTC AATTCGACCT 
CTCTTTCCTG CTGGCTATTT TTATGATACT CAGGTTCTCT GTAATCTGCT AGCAGTAGAT 
GGCATCAATG AACCTGATAT CTTAGCAATT AATGGCGCTT CTGCAGCTCT CTCCTTATCA 
GATATCCCTT GGAATGGACC TGTTGGGGCA GTACGAATAG GAATGATCGA TGGAGAATGT 
GTCGTTAACC CAACAAGGAA AGAAATGTCT TCTAGCACCT TAAATTTAGT AGTTGCTGGA 
GCACCTAAAA GCCAAATTGT TATGTTGGAA GCCTCTGCAG AAAACATTTT ACAGCAGGAC 
TTTTGCCATG CTATCAAAGT CGGGGTGAAG TATACGCAGC AGATAATTCA GAGCATCCAG 
CAGTTGGTAA AAGAAATCGG TGTTGCCAAG AGGACACCCC AGAAGATATT TACTCCTTCT 
GCAGAGATTG TGAAATACAC GCATACAATC GCCATGGAGA AACTCTATGC AGTTTTTACA 
GATTATGAAC ATGATAAAGT TTCCAGGGAT GAAGCTGTTA ACAAGATAAG ATTAGATACA 
GAGGAACATC TAAAGGAAAA ATTTCCAGAG GTTGACCAAC TTGAAATAAT AGAATCCTTC 
AACGTTGTTG CAAAGGAGGT TTTCAGAAGT ATTATTTTGA ATGAATACAA AAGGTGTGAT 
GGACGAGATC TGACTTCACT TAGGAATATA AGTTGCGAGG TAGATATGTT TAAAACACTT 
CATGGATCAG CATTATTTCA GAGAGGACAA ACACAGGTAC TCTGTACTGT TACATTTGAT 
TCATTAGAAT CCAGTATTAA ATCAGATCAA ATTATAACAG CTATAAATGG GGTAAAAGAT 
AAAAATTTCA TGTTGCACTA TGAGTTTCCT CCTTATGCAA CCAATGAAAC TGGCAAAGTT 
ACTGGTGTAA ATAGAAGAGA ACTTGGACAT GGTGCTCTTG CTGAGAAAGC TTTGTGTCCT 
GTTATTCCCA AAGATTTTCC TTTTACCATA AGAGTTACAT CTGAAGTTCT CGAGTCAAAC 
GGGTCATCTT CTATGGCATC TGCATGTGGT GGAAGTTTGG CATTAATGGA TGCAGGGGTC 
CCAATTTCAT CTGCTGTTGC AGGTGTAGCA GTGGGATTGG TTACCAAAAG CAATCCAGAG 
AAAGGTGAAA TAGAAGACTA TCGTTTGCTA ACAGATATTC TGGGAATTGA AGATTACAAT 
GGTGACATGG ATTTCAAAAT AGCTGGTACA AATAAAGGAA TAACTGCATT ACAGGCTGAT 
ATTAAGTTAC CTGGAGTACC AGTTAAAATT ATAATGGAAG CCATCCAACA AGCAACAGTG 
GCAAAGAGGG AAATATTGCA GACAATGAGC AAAACGATTT CAAAACCTCG AGCATCTAGG 
AAAGAAAATG GACCAGTTGT AGAAACAGTA AAGGTTCCAT TATCAAAACG AGCAAAATTC 
ATTGGGCCTG GTGGATATCA CTTAAAAAAA CTCCAGGCTG AGACAGGTGT AACAATTAGT 
CAGGTTGATG AAGAAACATT CTCCATATTT GCACCAACAC CTACTGCAAT GCATGAGGCA 
AGAGACTTCA TTACAGAAAT TTGCAGAGAT GATCAAGAGC AACAATTGGA ATTTGGAGCA 
GTTTATACCG CTACAATAAC TGAAATCAGA GACACAGGAG TAATGGTAAA ACTGTATCCA 
AACATGACTG CAGTGCTACT TCATAATTCA CAACTTGACC AACGAAAGAT TAAACATCCC 
ACTGCCCTGG GATTAGAAGT TGGCCAGGAA ATTCAGGTCA AATACTTTGG CCGTGATCCA 
GCTGATGGAA GAATGAGGCT TTCCCGTAAA GTACTTCAGT CTCCAGCTAC AACTGTTCTC 
AAAACTCTAA ATGATAGGAG CAGCATTGTA ATGGGAGAGC CTATTTCACA GTCATCATCT 
AACTCTTCCC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135843.1
CDS	11..2365
Translation	MAACRPCCLCLCLRPLSCGPLGRPRRHRALSYLQVRALWSGTRSRAVTVDLGHRKLEISSGKLARFADGCAVVQSGDTAVMVTAVSKTKPSPSQFMPLVVDYRQKAAAAGRIPTNYLRREIGSSDKEILTSRVIDRSIRPLFPAGYFYDTQVLCNLLAVDGINEPDILAINGASAALSLSDIPWNGPVGAVRIGMIDGECVVNPTRKEMSSSTLNLVVAGAPKSQIVMLEASAENILQQDFCHAIKVGVKYTQQIIQSIQQLVKEIGVAKRTPQKIFTPSAEIVKYTHTIAMEKLYAVFTDYEHDKVSRDEAVNKIRLDTEEHLKEKFPEVDQLEIIESFNVVAKEVFRSIILNEYKRCDGRDLTSLRNISCEVDMFKTLHGSALFQRGQTQVLCTVTFDSLESSIKSDQIITAINGVKDKNFMLHYEFPPYATNETGKVTGVNRRELGHGALAEKALCPVIPKDFPFTIRVTSEVLESNGSSSMASACGGSLALMDAGVPISSAVAGVAVGLVTKSNPEKGEIEDYRLLTDILGIEDYNGDMDFKIAGTNKGITALQADIKLPGVPVKIIMEAIQQATVAKREILQTMSKTISKPRASRKENGPVVETVKVPLSKRAKFIGPGGYHLKKLQAETGVTISQVDEETFSIFAPTPTAMHEARDFITEICRDDQEQQLEFGAVYTATITEIRDTGVMVKLYPNMTAVLLHNSQLDQRKIKHPTALGLEVGQEIQVKYFGRDPADGRMRLSRKVLQSPATTVLKTLNDRSSIVMGEPISQSSSNSSP

Target ORF information:

RefSeq Version	NM_001142371.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polyribonucleotide nucleotidyltransferase 1 (Pnpt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142371.1

ORF Insert Sequence:

ATGGCGGCTT GCAGGCCGTG CTGCTTGTGC CTGTGTCTCC GACCGCTGAG CTGCGGTCCC 
CTCGGCCGGC CAAGGCGGCA TCGTGCACTC AGCTATTTGC AGGTGCGAGC GCTGTGGAGC 
GGCACCCGGT CCAGAGCAGT GACTGTGGAC TTGGGACACA GAAAATTAGA AATATCCTCT 
GGAAAACTGG CAAGATTTGC TGATGGCTGT GCTGTGGTAC AGTCAGGTGA TACTGCAGTA 
ATGGTCACAG CGGTCAGCAA AACAAAACCT TCACCTTCCC AATTCATGCC ATTGGTGGTA 
GACTACAGAC AGAAGGCTGC TGCAGCAGGT AGAATCCCCA CAAACTACCT TAGAAGAGAG 
ATTGGCTCTT CTGACAAAGA GATTCTTACA AGTCGAGTAA TAGATCGTTC AATTCGACCT 
CTCTTTCCTG CTGGCTATTT TTATGATACT CAGGTTCTCT GTAATCTGCT AGCAGTAGAT 
GGCATCAATG AACCTGATAT CTTAGCAATT AATGGCGCTT CTGCAGCTCT CTCCTTATCA 
GATATCCCTT GGAATGGACC TGTTGGGGCA GTACGAATAG GAATGATCGA TGGAGAATGT 
GTCGTTAACC CAACAAGGAA AGAAATGTCT TCTAGCACCT TAAATTTAGT AGTTGCTGGA 
GCACCTAAAA GCCAAATTGT TATGTTGGAA GCCTCTGCAG AAAACATTTT ACAGCAGGAC 
TTTTGCCATG CTATCAAAGT CGGGGTGAAG TATACGCAGC AGATAATTCA GAGCATCCAG 
CAGTTGGTAA AAGAAATCGG TGTTGCCAAG AGGACACCCC AGAAGATATT TACTCCTTCT 
GCAGAGATTG TGAAATACAC GCATACAATC GCCATGGAGA AACTCTATGC AGTTTTTACA 
GATTATGAAC ATGATAAAGT TTCCAGGGAT GAAGCTGTTA ACAAGATAAG ATTAGATACA 
GAGGAACATC TAAAGGAAAA ATTTCCAGAG GTTGACCAAC TTGAAATAAT AGAATCCTTC 
AACGTTGTTG CAAAGGAGGT TTTCAGAAGT ATTATTTTGA ATGAATACAA AAGGTGTGAT 
GGACGAGATC TGACTTCACT TAGGAATATA AGTTGCGAGG TAGATATGTT TAAAACACTT 
CATGGATCAG CATTATTTCA GAGAGGACAA ACACAGGTAC TCTGTACTGT TACATTTGAT 
TCATTAGAAT CCAGTATTAA ATCAGATCAA ATTATAACAG CTATAAATGG GGTAAAAGAT 
AAAAATTTCA TGTTGCACTA TGAGTTTCCT CCTTATGCAA CCAATGAAAC TGGCAAAGTT 
ACTGGTGTAA ATAGAAGAGA ACTTGGACAT GGTGCTCTTG CTGAGAAAGC TTTGTGTCCT 
GTTATTCCCA AAGATTTTCC TTTTACCATA AGAGTTACAT CTGAAGTTCT CGAGTCAAAC 
GGGTCATCTT CTATGGCATC TGCATGTGGT GGAAGTTTGG CATTAATGGA TGCAGGGGTC 
CCAATTTCAT CTGCTGTTGC AGGTGTAGCA GTGGGATTGG TTACCAAAAG CAATCCAGAG 
AAAGGTGAAA TAGAAGACTA TCGTTTGCTA ACAGATATTC TGGGAATTGA AGATTACAAT 
GGTGACATGG ATTTCAAAAT AGCTGGTACA AATAAAGGAA TAACTGCATT ACAGGCTGAT 
ATTAAGTTAC CTGGAGTACC AGTTAAAATT ATAATGGAAG CCATCCAACA AGCAACAGTG 
GCAAAGAGGG AAATATTGCA GACAATGAGC AAAACGATTT CAAAACCTCG AGCATCTAGG 
AAAGAAAATG GACCAGTTGT AGAAACAGTA AAGGTTCCAT TATCAAAACG AGCAAAATTC 
ATTGGGCCTG GTGGATATCA CTTAAAAAAA CTCCAGGCTG AGACAGGTGT AACAATTAGT 
CAGGTTGATG AAGAAACATT CTCCATATTT GCACCAACAC CTACTGCAAT GCATGAGGCA 
AGAGACTTCA TTACAGAAAT TTGCAGAGAT GATCAAGAGC AACAATTGGA ATTTGGAGCA 
GTTTATACCG CTACAATAAC TGAAATCAGA GACACAGGAG TAATGGTAAA ACTGTATCCA 
AACATGACTG CAGTGCTACT TCATAATTCA CAACTTGACC AACGAAAGAT TAAACATCCC 
ACTGCCCTGG GATTAGAAGT TGGCCAGGAA ATTCAGGTCA AATACTTTGG CCGTGATCCA 
GCTGATGGAA GAATGAGGCT TTCCCGTAAA GTACTTCAGT CTCCAGCTAC AACTGTTCTC 
AAAACTCTAA ATGATAGGAG CAGCATTGTA ATGGGAGAGC CTATTTCACA GTCATCATCT 
AACTCTTCCC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss.
American journal of human genetics91(5)919-27(2012 Nov)
von Ameln S,Wang G,Boulouiz R,Rutherford MA,Smith GM,Li Y,Pogoda HM,N?rnberg G,Stiller B,Volk AE,Borck G,Hong JS,Goodyear RJ,Abidi O,N?rnberg P,Hofmann K,Richardson GP,Hammerschmidt M,Moser T,Wollnik B,Koehler CM,Teitell MA,Barakat A,Kubisch C

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

PNPASE regulates RNA import into mitochondria.
Cell142(3)456-67(2010 Aug)
Wang G,Chen HW,Oktay Y,Zhang J,Allen EL,Smith GM,Fan KC,Hong JS,French SW,McCaffery JM,Lightowlers RN,Morse HC 3rd,Koehler CM,Teitell MA

Human polynucleotide phosphorylase selectively and preferentially degrades microRNA-221 in human melanoma cells.
Proceedings of the National Academy of Sciences of the United States of America107(26)11948-53(2010 Jun)
Das SK,Sokhi UK,Bhutia SK,Azab B,Su ZZ,Sarkar D,Fisher PB

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Pnpt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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