pnpt1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pnpt1
Entrez Gene ID	100750260
Full Name	polyribonucleotide nucleotidyltransferase 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001251832.1	NP_001238761.1	polyribonucleotide nucleotidyltransferase 1, mitochondrial

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PNPT1	XP_538497.3
Bos taurus (cattle)	PNPT1	NP_001179126.1
Drosophila melanogaster (fruit fly)	CG11337	NP_001097990.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010791	XP_318517.3
Homo sapiens (human)	PNPT1	NP_149100.2
	PNPT1	XP_001112421.1
Danio rerio (zebrafish)	pnpt1	NP_001238761.1
Gallus gallus (chicken)	PNPT1	XP_419282.4
Caenorhabditis elegans (roundworm)	BE0003N10.1	NP_497324.3
Xenopus tropicalis (tropical clawed frog)	pnpt1	NP_001017033.1
Pan troglodytes (chimpanzee)	PNPT1	XP_515490.2
Mus musculus (house mouse)	Pnpt1	NP_082145.1
Rattus norvegicus (Norway rat)	Pnpt1	NP_001135843.1
Arabidopsis thaliana (thale cress)	AT5G14580	NP_196962.1

Gene Ontology
Bibliography

Related articles in PubMed

A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss.
von Ameln S, Wang G, Boulouiz R, Rutherford MA, Smith GM, Li Y, Pogoda HM, N?rnberg G, Stiller B, Volk AE, Borck G, Hong JS, Goodyear RJ, Abidi O, N?rnberg P, Hofmann K, Richardson GP, Hammerschmidt M, Moser T, Wollnik B, Koehler CM, Teitell MA, Barakat A, Kubisch C
American journal of human genetics91(5)919-27(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pnpt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pnpt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa30370	NM_001251832.1 Latest version!	Danio rerio polyribonucleotide nucleotidyltransferase 1 (pnpt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pnpt1 ( NM_001251832.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001238761.1 Danio rerio polyribonucleotide nucleotidyltransferase 1 (pnpt1), mRNA.

Price & Availability↑

CloneID	ODa30370
Clone ID Related Accession (Same CDS sequence)	NM_001251832.1
Accession Version	NM_001251832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-04
Organism	Danio rerio(zebrafish)
Product	polyribonucleotide nucleotidyltransferase 1, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JN381023.1. ##Evidence-Data-START## Transcript exon combination :: JN381023.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGAATGTGT GTGTATGTGA GAGGCTGAGG ATGATGAAGA GTGTGAGGAT GATGAAGATG 
ATGATGAAGA CTCGCCTCTG CTGGGCGCGG GTGTGTGCGG TCAGGGGGAT TCAGCAGCAC 
AGCGCCACCG TCACACTAGG AGACAGGAAG TTGGAGATCT CCACTGGGAA ACTGGCCCGC 
TTCTCTGACG GCTGTGCAGT GGTCAAGTCG GGTGAGACGT CAGTGATGGT GACAGCGGTC 
AGTAAGAGCA GACCGGCCGC CGCTCAGTTC ATGCCGCTTG TGGTGGATTA CAGACAGAAG 
GCAGCAGCGG CTGGGCGAAT CCCCACTAAC CATCTGAGAC GAGAGCTGGG CACCACTGAC 
ACCGAGATCC TGACCAGCAG ACTCATCGAT CGATCCATTA GGCCGCTGTT TCCTGCTGGA 
TACTTCTACG ACACACAGGT GATGTGTAAC ATACTGGCGG CTGATGGAGT CAACGATCCT 
GACGTTCTGG CCATTAATGG AGCGTCTGCA GCTCTGACTC TCTCTGATAT TCCCTGGAAC 
GGCCCCATCG GAGCGGTGCG TGTGGGTCTG CTGGACGGGG AGTTCCTCAT TAACCCGTCC 
CGCTCTGAGA TGAACCGCAG CTCCCTGAAC CTCGTGATCG CCGCCGCGCC GTCCAGCCAT 
GTGGTGATGA TAGAGGCAGC AGCAGAGAAC ATCCTCCAGC AGGACTTCTG TCACGCGGTG 
AAGCTGGGAG TGAAACACAC ACAGCAGATC ATCCAGAGCC TGCAGCAGAT CAGCAGAGAC 
ATGAAGATCA GCAAGAGGAG CAGCAGACTG TACACCGCTG CAGCAGACAT GCAGGAGCAC 
ACACGCCTGC TGGCGTCTGA CAGGATCTAC GCTGTGTTTA CAGACTTCAC ACATGACAAG 
ATTTCTAGAG ACGAGGCGAT CAATAAGATC CGTCTGGAGG CCGAGGAGAA GATCAGAGAG 
AAGTTCCCAC ATGCAGAGCC GTTCGAGGTG ATGGAGGCCT TCAACAGCGT CTCCAAGGAG 
ATCTTCAGGA AGCTGGTGCT GCAGGAGTAC AGGCGGTGTG ACGGCAGAGA TCTGACCGCT 
CTCCGCAACA TCTCCTGTGA GGTGGACGTG TTCAAACCGC TGCACGGCTC CGCACTGTTC 
CAGAGGGGTC AAACACAGGT GCTGTGTTCA GTGACTTTTG ACTCTCTGGA GTCCAGTTTG 
AAGACAGACG TGATCACCTC AGCGCTCAGT GGAGTCAAAG ACAAGAACTT CCTGCTGCAC 
TATGAGTTTC CTCCCTACGC CACTAACGAG ATCGGCAGAA CCGGCGGAGC CAACCGCAGA 
GAGCTGGGCC ACGGAGCGCT AGCGGAGAAG GCTCTGAGAC CCGTCATCCC CTCCAGTTTC 
CCCTTCACCA TCCGCGTCAC CTCAGAAGTG CTGGAGTCTA ACGGCTCGTC CTCGATGGCC 
TCAGTGTGTG GAGGCAGTCT GGCACTGATG GACGCAGGTG TGCCGATCTC CTCTCCAGTA 
GCCGGAGTGG CCATCGGCCT GATCTCTGAG GCTCATCCTG ACAGGCCGTC GGAGATCGAG 
AGCTACCGGC TGCTGACGGA CATCCTGGGA ATAGAGGACT ACAATGGAGA CATGGACTTC 
AAGATGGCGG GCAGCAGCAA AGGCATCACT GCTCTGCAGG CCGATGTCAA GATACCAGGA 
CTTCCTCTGA AGATCGTGAT GGAGGCCATA CAGCAGGCCA CAGTGGCCAA GCGAGAGATT 
CTGGGCATCA TGGGTCAGTG CATCTCCCGA CCGCGGAGTT CCAGGAAGGA GAACGGGCCT 
GTTGTGGAGA ACATCACAGT GCCGTTATCC AGAAGAGCGC TGTTCATCGG CCCGGGAGGC 
ATAAACCTGC GCAGACTGCA GGCTCAGACG GGTGTGACCA TCAGTCAGGT AGACGAGCAG 
ACGTTCTCAG TGTTCGCTCC CACACCTGCA GCCATGAGTG AAGCACAGGA GATCATCAAA 
GACACCTGCA GAGACGATCA GGAGCAGCAG CTGGAGTTCG GCGCCATTTA CACAGCCACC 
ATCACTGAGA TCAGGGATGT GGGTGTGATG GTGAAGCTGT ATCCCAACAT GAGTCCGGTT 
CTGCTGCACA ACTCACAGCT GGACCACAAG CGGATTCAGC ACCCCAGCGC TCTCGGCCTG 
GATGTGGGTC AGCAGATACA GGTGAAGTAT TTCGGCCGTG ACCCGACAGA CGGCAGGATG 
AGGCTCTCCC GGAAGGTGCT GCTGTCCCCG ACGGCCACGC TGGCCAAGAG TCTGAGCGAG 
AGACACAGCA TCTCTGTGGG ATCCTCCAGC CAGAACCCCG CCGACCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238761.1
CDS	1..2331
Translation	MNVCVCERLRMMKSVRMMKMMMKTRLCWARVCAVRGIQQHSATVTLGDRKLEISTGKLARFSDGCAVVKSGETSVMVTAVSKSRPAAAQFMPLVVDYRQKAAAAGRIPTNHLRRELGTTDTEILTSRLIDRSIRPLFPAGYFYDTQVMCNILAADGVNDPDVLAINGASAALTLSDIPWNGPIGAVRVGLLDGEFLINPSRSEMNRSSLNLVIAAAPSSHVVMIEAAAENILQQDFCHAVKLGVKHTQQIIQSLQQISRDMKISKRSSRLYTAAADMQEHTRLLASDRIYAVFTDFTHDKISRDEAINKIRLEAEEKIREKFPHAEPFEVMEAFNSVSKEIFRKLVLQEYRRCDGRDLTALRNISCEVDVFKPLHGSALFQRGQTQVLCSVTFDSLESSLKTDVITSALSGVKDKNFLLHYEFPPYATNEIGRTGGANRRELGHGALAEKALRPVIPSSFPFTIRVTSEVLESNGSSSMASVCGGSLALMDAGVPISSPVAGVAIGLISEAHPDRPSEIESYRLLTDILGIEDYNGDMDFKMAGSSKGITALQADVKIPGLPLKIVMEAIQQATVAKREILGIMGQCISRPRSSRKENGPVVENITVPLSRRALFIGPGGINLRRLQAQTGVTISQVDEQTFSVFAPTPAAMSEAQEIIKDTCRDDQEQQLEFGAIYTATITEIRDVGVMVKLYPNMSPVLLHNSQLDHKRIQHPSALGLDVGQQIQVKYFGRDPTDGRMRLSRKVLLSPTATLAKSLSERHSISVGSSSQNPADP

Target ORF information:

RefSeq Version	NM_001251832.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio polyribonucleotide nucleotidyltransferase 1 (pnpt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001251832.1

ORF Insert Sequence:

ATGAATGTGT GTGTATGTGA GAGGCTGAGG ATGATGAAGA GTGTGAGGAT GATGAAGATG 
ATGATGAAGA CTCGCCTCTG CTGGGCGCGG GTGTGTGCGG TCAGGGGGAT TCAGCAGCAC 
AGCGCCACCG TCACACTAGG AGACAGGAAG TTGGAGATCT CCACTGGGAA ACTGGCCCGC 
TTCTCTGACG GCTGTGCAGT GGTCAAGTCG GGTGAGACGT CAGTGATGGT GACAGCGGTC 
AGTAAGAGCA GACCGGCCGC CGCTCAGTTC ATGCCGCTTG TGGTGGATTA CAGACAGAAG 
GCAGCAGCGG CTGGGCGAAT CCCCACTAAC CATCTGAGAC GAGAGCTGGG CACCACTGAC 
ACCGAGATCC TGACCAGCAG ACTCATCGAT CGATCCATTA GGCCGCTGTT TCCTGCTGGA 
TACTTCTACG ACACACAGGT GATGTGTAAC ATACTGGCGG CTGATGGAGT CAACGATCCT 
GACGTTCTGG CCATTAATGG AGCGTCTGCA GCTCTGACTC TCTCTGATAT TCCCTGGAAC 
GGCCCCATCG GAGCGGTGCG TGTGGGTCTG CTGGACGGGG AGTTCCTCAT TAACCCGTCC 
CGCTCTGAGA TGAACCGCAG CTCCCTGAAC CTCGTGATCG CCGCCGCGCC GTCCAGCCAT 
GTGGTGATGA TAGAGGCAGC AGCAGAGAAC ATCCTCCAGC AGGACTTCTG TCACGCGGTG 
AAGCTGGGAG TGAAACACAC ACAGCAGATC ATCCAGAGCC TGCAGCAGAT CAGCAGAGAC 
ATGAAGATCA GCAAGAGGAG CAGCAGACTG TACACCGCTG CAGCAGACAT GCAGGAGCAC 
ACACGCCTGC TGGCGTCTGA CAGGATCTAC GCTGTGTTTA CAGACTTCAC ACATGACAAG 
ATTTCTAGAG ACGAGGCGAT CAATAAGATC CGTCTGGAGG CCGAGGAGAA GATCAGAGAG 
AAGTTCCCAC ATGCAGAGCC GTTCGAGGTG ATGGAGGCCT TCAACAGCGT CTCCAAGGAG 
ATCTTCAGGA AGCTGGTGCT GCAGGAGTAC AGGCGGTGTG ACGGCAGAGA TCTGACCGCT 
CTCCGCAACA TCTCCTGTGA GGTGGACGTG TTCAAACCGC TGCACGGCTC CGCACTGTTC 
CAGAGGGGTC AAACACAGGT GCTGTGTTCA GTGACTTTTG ACTCTCTGGA GTCCAGTTTG 
AAGACAGACG TGATCACCTC AGCGCTCAGT GGAGTCAAAG ACAAGAACTT CCTGCTGCAC 
TATGAGTTTC CTCCCTACGC CACTAACGAG ATCGGCAGAA CCGGCGGAGC CAACCGCAGA 
GAGCTGGGCC ACGGAGCGCT AGCGGAGAAG GCTCTGAGAC CCGTCATCCC CTCCAGTTTC 
CCCTTCACCA TCCGCGTCAC CTCAGAAGTG CTGGAGTCTA ACGGCTCGTC CTCGATGGCC 
TCAGTGTGTG GAGGCAGTCT GGCACTGATG GACGCAGGTG TGCCGATCTC CTCTCCAGTA 
GCCGGAGTGG CCATCGGCCT GATCTCTGAG GCTCATCCTG ACAGGCCGTC GGAGATCGAG 
AGCTACCGGC TGCTGACGGA CATCCTGGGA ATAGAGGACT ACAATGGAGA CATGGACTTC 
AAGATGGCGG GCAGCAGCAA AGGCATCACT GCTCTGCAGG CCGATGTCAA GATACCAGGA 
CTTCCTCTGA AGATCGTGAT GGAGGCCATA CAGCAGGCCA CAGTGGCCAA GCGAGAGATT 
CTGGGCATCA TGGGTCAGTG CATCTCCCGA CCGCGGAGTT CCAGGAAGGA GAACGGGCCT 
GTTGTGGAGA ACATCACAGT GCCGTTATCC AGAAGAGCGC TGTTCATCGG CCCGGGAGGC 
ATAAACCTGC GCAGACTGCA GGCTCAGACG GGTGTGACCA TCAGTCAGGT AGACGAGCAG 
ACGTTCTCAG TGTTCGCTCC CACACCTGCA GCCATGAGTG AAGCACAGGA GATCATCAAA 
GACACCTGCA GAGACGATCA GGAGCAGCAG CTGGAGTTCG GCGCCATTTA CACAGCCACC 
ATCACTGAGA TCAGGGATGT GGGTGTGATG GTGAAGCTGT ATCCCAACAT GAGTCCGGTT 
CTGCTGCACA ACTCACAGCT GGACCACAAG CGGATTCAGC ACCCCAGCGC TCTCGGCCTG 
GATGTGGGTC AGCAGATACA GGTGAAGTAT TTCGGCCGTG ACCCGACAGA CGGCAGGATG 
AGGCTCTCCC GGAAGGTGCT GCTGTCCCCG ACGGCCACGC TGGCCAAGAG TCTGAGCGAG 
AGACACAGCA TCTCTGTGGG ATCCTCCAGC CAGAACCCCG CCGACCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss.
American journal of human genetics91(5)919-27(2012 Nov)
von Ameln S,Wang G,Boulouiz R,Rutherford MA,Smith GM,Li Y,Pogoda HM,N?rnberg G,Stiller B,Volk AE,Borck G,Hong JS,Goodyear RJ,Abidi O,N?rnberg P,Hofmann K,Richardson GP,Hammerschmidt M,Moser T,Wollnik B,Koehler CM,Teitell MA,Barakat A,Kubisch C

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pnpt1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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