TLR4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TLR4
Entrez Gene ID	7099
Full Name	toll like receptor 4
Synonyms	ARMD10,CD284,TLR-4,TOLL
General protein information	Preferred Names toll like receptor 4 Names toll-like receptor 4 hToll homolog of Drosophila toll
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q33.1
Summary	The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012].
Disorder MIM:	603030

mRNA and Protein(s)

mRNA	Protein	Name
NM_138554.4	NP_612564.1	toll-like receptor 4 isoform A precursor
NM_003266.4	NP_003257.1	toll-like receptor 4 isoform C
NM_138557.2	NP_612567.1	toll-like receptor 4 isoform D
NM_138554.5	NP_612564.1	toll-like receptor 4 isoform A precursor
NM_138557.3	NP_612567.1	toll-like receptor 4 isoform D
NM_003266.3	NP_003257.1	toll-like receptor 4 isoform C

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tlr4	NP_067272.1
Danio rerio (zebrafish)	tlr4ba	NP_001124523.1
Pan troglodytes (chimpanzee)	TLR4	NP_001138335.1
Gallus gallus (chicken)	TLR4	NP_001025864.1
Homo sapiens (human)	TLR4	NP_612564.1
	TLR4	NP_001032169.1
Rattus norvegicus (Norway rat)	Tlr4	NP_062051.1
Danio rerio (zebrafish)	tlr4al	XP_001919699.4
Danio rerio (zebrafish)	tlr4bb	NP_997978.1
Canis lupus familiaris (dog)	TLR4	NP_001002950.2
Bos taurus (cattle)	TLR4	NP_776623.5

Gene Ontology

The following TLR4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLR4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26864	NM_138554.5 Latest version!	Homo sapiens toll like receptor 4 (TLR4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu27172	NM_138557.3 Latest version!	Homo sapiens toll like receptor 4 (TLR4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu27264	NM_003266.4 Latest version!	Homo sapiens toll like receptor 4 (TLR4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
View Old Accession Versions >>
OHu26864	NM_138554.4	Homo sapiens toll like receptor 4 (TLR4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu27172	NM_138557.2	Homo sapiens toll like receptor 4 (TLR4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu27264	NM_003266.3	Homo sapiens toll like receptor 4 (TLR4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLR4 ( NM_138554.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_612564.1 Homo sapiens toll like receptor 4 (TLR4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26864
Clone ID Related Accession (Same CDS sequence)	NM_138554.4 , NM_138554.5
Accession Version	NM_138554.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	toll-like receptor 4 isoform A precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA187988.1, BC117422.1, AK226155.1, BG622357.1 and DA218006.1. On Jan 21, 2012 this sequence version replaced NM_138554.3. Transcript Variant: This variant (1) encodes the longest isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.283490.1, SRR1803615.39895.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGTCTG CCTCGCGCCT GGCTGGGACT CTGATCCCAG CCATGGCCTT CCTCTCCTGC 
GTGAGACCAG AAAGCTGGGA GCCCTGCGTG GAGGTGGTTC CTAATATTAC TTATCAATGC 
ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC 
CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG 
CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC 
CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA 
GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT 
CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT 
CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG 
GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA 
ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA 
GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA 
AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG 
GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG 
TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT 
ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT 
GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT 
AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC 
AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC 
AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC 
CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC 
TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT 
TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA 
GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC 
AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC 
CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC 
AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT 
AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA 
AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC 
TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC 
TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG 
CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT 
GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT 
CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC 
TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG 
CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC 
AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC 
TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG 
AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG 
CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT 
GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA 
TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612564.1
CDS	299..2818
Translation	MMSASRLAGTLIPAMAFLSCVRPESWEPCVEVVPNITYQCMELNFYKIPDNLPFSTKNLDLSFNPLRHLGSYSFFSFPELQVLDLSRCEIQTIEDGAYQSLSHLSTLILTGNPIQSLALGAFSGLSSLQKLVAVETNLASLENFPIGHLKTLKELNVAHNLIQSFKLPEYFSNLTNLEHLDLSSNKIQSIYCTDLRVLHQMPLLNLSLDLSLNPMNFIQPGAFKEIRLHKLTLRNNFDSLNVMKTCIQGLAGLEVHRLVLGEFRNEGNLEKFDKSALEGLCNLTIEEFRLAYLDYYLDDIIDLFNCLTNVSSFSLVSVTIERVKDFSYNFGWQHLELVNCKFGQFPTLKLKSLKRLTFTSNKGGNAFSEVDLPSLEFLDLSRNGLSFKGCCSQSDFGTTSLKYLDLSFNGVITMSSNFLGLEQLEHLDFQHSNLKQMSEFSVFLSLRNLIYLDISHTHTRVAFNGIFNGLSSLEVLKMAGNSFQENFLPDIFTELRNLTFLDLSQCQLEQLSPTAFNSLSSLQVLNMSHNNFFSLDTFPYKCLNSLQVLDYSLNHIMTSKKQELQHFPSSLAFLNLTQNDFACTCEHQSFLQWIKDQRQLLVEVERMECATPSDKQGMPVLSLNITCQMNKTIIGVSVLSVLVVSVVAVLVYKFYFHLMLLAGCIKYGRGENIYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIHEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKVEKTLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLRKALLDGKSWNPEGTVGTGCNWQEATSI

Target ORF information:

RefSeq Version	NM_138554.4
Organism	Homo sapiens(human)
Definition	Homo sapiens toll like receptor 4 (TLR4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138554.4

ORF Insert Sequence:

ATGATGTCTG CCTCGCGCCT GGCTGGGACT CTGATCCCAG CCATGGCCTT CCTCTCCTGC 
GTGAGACCAG AAAGCTGGGA GCCCTGCGTG GAGGTGGTTC CTAATATTAC TTATCAATGC 
ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC 
CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG 
CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC 
CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA 
GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT 
CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT 
CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG 
GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA 
ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA 
GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA 
AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG 
GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG 
TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT 
ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT 
GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT 
AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC 
AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC 
AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC 
CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC 
TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT 
TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA 
GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC 
AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC 
CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC 
AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT 
AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA 
AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC 
TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC 
TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG 
CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT 
GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT 
CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC 
TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG 
CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC 
AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC 
TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG 
AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG 
CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT 
GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA 
TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR4 ( NM_138554.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_612564.1 Homo sapiens toll like receptor 4 (TLR4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26864
Clone ID Related Accession (Same CDS sequence)	NM_138554.4 , NM_138554.5
Accession Version	NM_138554.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	toll-like receptor 4 isoform A precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA187988.1, BC117422.1, AK226155.1, BG622357.1, DA218006.1 and AL160272.16. On Nov 22, 2018 this sequence version replaced NM_138554.4. Transcript Variant: This variant (1) encodes the longest isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U93091.1, SRR1803613.20869.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000355622.8/ ENSP00000363089.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATGTCTG CCTCGCGCCT GGCTGGGACT CTGATCCCAG CCATGGCCTT CCTCTCCTGC 
GTGAGACCAG AAAGCTGGGA GCCCTGCGTG GAGGTGGTTC CTAATATTAC TTATCAATGC 
ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC 
CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG 
CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC 
CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA 
GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT 
CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT 
CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG 
GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA 
ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA 
GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA 
AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG 
GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG 
TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT 
ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT 
GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT 
AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC 
AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC 
AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC 
CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC 
TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT 
TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA 
GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC 
AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC 
CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC 
AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT 
AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA 
AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC 
TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC 
TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG 
CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT 
GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT 
CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC 
TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG 
CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC 
AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC 
TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG 
AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG 
CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT 
GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA 
TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612564.1
CDS	71..2590
Translation	MMSASRLAGTLIPAMAFLSCVRPESWEPCVEVVPNITYQCMELNFYKIPDNLPFSTKNLDLSFNPLRHLGSYSFFSFPELQVLDLSRCEIQTIEDGAYQSLSHLSTLILTGNPIQSLALGAFSGLSSLQKLVAVETNLASLENFPIGHLKTLKELNVAHNLIQSFKLPEYFSNLTNLEHLDLSSNKIQSIYCTDLRVLHQMPLLNLSLDLSLNPMNFIQPGAFKEIRLHKLTLRNNFDSLNVMKTCIQGLAGLEVHRLVLGEFRNEGNLEKFDKSALEGLCNLTIEEFRLAYLDYYLDDIIDLFNCLTNVSSFSLVSVTIERVKDFSYNFGWQHLELVNCKFGQFPTLKLKSLKRLTFTSNKGGNAFSEVDLPSLEFLDLSRNGLSFKGCCSQSDFGTTSLKYLDLSFNGVITMSSNFLGLEQLEHLDFQHSNLKQMSEFSVFLSLRNLIYLDISHTHTRVAFNGIFNGLSSLEVLKMAGNSFQENFLPDIFTELRNLTFLDLSQCQLEQLSPTAFNSLSSLQVLNMSHNNFFSLDTFPYKCLNSLQVLDYSLNHIMTSKKQELQHFPSSLAFLNLTQNDFACTCEHQSFLQWIKDQRQLLVEVERMECATPSDKQGMPVLSLNITCQMNKTIIGVSVLSVLVVSVVAVLVYKFYFHLMLLAGCIKYGRGENIYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIHEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKVEKTLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLRKALLDGKSWNPEGTVGTGCNWQEATSI

Target ORF information:

RefSeq Version	NM_138554.5
Organism	Homo sapiens(human)
Definition	Homo sapiens toll like receptor 4 (TLR4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138554.5

ORF Insert Sequence:

ATGATGTCTG CCTCGCGCCT GGCTGGGACT CTGATCCCAG CCATGGCCTT CCTCTCCTGC 
GTGAGACCAG AAAGCTGGGA GCCCTGCGTG GAGGTGGTTC CTAATATTAC TTATCAATGC 
ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC 
CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG 
CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC 
CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA 
GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT 
CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT 
CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG 
GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA 
ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA 
GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA 
AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG 
GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG 
TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT 
ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT 
GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT 
AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC 
AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC 
AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC 
CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC 
TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT 
TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA 
GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC 
AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC 
CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC 
AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT 
AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA 
AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC 
TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC 
TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG 
CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT 
GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT 
CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC 
TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG 
CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC 
AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC 
TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG 
AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG 
CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT 
GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA 
TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR4 ( NM_138557.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_612567.1 Homo sapiens toll like receptor 4 (TLR4), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu27172
Clone ID Related Accession (Same CDS sequence)	NM_138557.2 , NM_138557.3
Accession Version	NM_138557.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	toll-like receptor 4 isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA187988.1, BC025294.1, AK226155.1, BG622357.1 and DA218006.1. On or before Jan 21, 2012 this sequence version replaced NR_024169.1, NM_138557.1. Transcript Variant: This variant (4) lacks an internal exon compared to transcript variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a weak Kozak sequence and a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG, which results in an isoform (D) with a shorter N-terminus compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA187988.1, SRR1803614.111988.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA 
GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA 
AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG 
GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG 
TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT 
ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT 
GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT 
AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC 
AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC 
AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC 
CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC 
TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT 
TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA 
GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC 
AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC 
CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC 
AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT 
AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA 
AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC 
TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC 
TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG 
CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT 
GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT 
CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC 
TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG 
CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC 
AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC 
TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG 
AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG 
CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT 
GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA 
TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612567.1
CDS	732..2651
Translation	MPLLNLSLDLSLNPMNFIQPGAFKEIRLHKLTLRNNFDSLNVMKTCIQGLAGLEVHRLVLGEFRNEGNLEKFDKSALEGLCNLTIEEFRLAYLDYYLDDIIDLFNCLTNVSSFSLVSVTIERVKDFSYNFGWQHLELVNCKFGQFPTLKLKSLKRLTFTSNKGGNAFSEVDLPSLEFLDLSRNGLSFKGCCSQSDFGTTSLKYLDLSFNGVITMSSNFLGLEQLEHLDFQHSNLKQMSEFSVFLSLRNLIYLDISHTHTRVAFNGIFNGLSSLEVLKMAGNSFQENFLPDIFTELRNLTFLDLSQCQLEQLSPTAFNSLSSLQVLNMSHNNFFSLDTFPYKCLNSLQVLDYSLNHIMTSKKQELQHFPSSLAFLNLTQNDFACTCEHQSFLQWIKDQRQLLVEVERMECATPSDKQGMPVLSLNITCQMNKTIIGVSVLSVLVVSVVAVLVYKFYFHLMLLAGCIKYGRGENIYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIHEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKVEKTLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLRKALLDGKSWNPEGTVGTGCNWQEATSI

Target ORF information:

RefSeq Version	NM_138557.2
Organism	Homo sapiens(human)
Definition	Homo sapiens toll like receptor 4 (TLR4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138557.2

ORF Insert Sequence:

ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA 
GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA 
AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG 
GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG 
TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT 
ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT 
GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT 
AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC 
AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC 
AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC 
CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC 
TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT 
TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA 
GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC 
AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC 
CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC 
AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT 
AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA 
AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC 
TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC 
TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG 
CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT 
GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT 
CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC 
TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG 
CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC 
AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC 
TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG 
AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG 
CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT 
GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA 
TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR4 ( NM_138557.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_612567.1 Homo sapiens toll like receptor 4 (TLR4), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu27172
Clone ID Related Accession (Same CDS sequence)	NM_138557.2 , NM_138557.3
Accession Version	NM_138557.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	toll-like receptor 4 isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC025294.1, AK226155.1, BG622357.1, DA218006.1 and AL160272.16. On May 31, 2019 this sequence version replaced NM_138557.2. Transcript Variant: This variant (4) lacks an internal exon compared to transcript variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a weak Kozak sequence and a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG, which results in an isoform (D) with a shorter N-terminus compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.112207.1, SRR1660807.89521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA 
GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA 
AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG 
GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG 
TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT 
ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT 
GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT 
AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC 
AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC 
AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC 
CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC 
TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT 
TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA 
GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC 
AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC 
CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC 
AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT 
AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA 
AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC 
TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC 
TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG 
CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT 
GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT 
CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC 
TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG 
CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC 
AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC 
TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG 
AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG 
CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT 
GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA 
TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612567.1
CDS	504..2423
Translation	MPLLNLSLDLSLNPMNFIQPGAFKEIRLHKLTLRNNFDSLNVMKTCIQGLAGLEVHRLVLGEFRNEGNLEKFDKSALEGLCNLTIEEFRLAYLDYYLDDIIDLFNCLTNVSSFSLVSVTIERVKDFSYNFGWQHLELVNCKFGQFPTLKLKSLKRLTFTSNKGGNAFSEVDLPSLEFLDLSRNGLSFKGCCSQSDFGTTSLKYLDLSFNGVITMSSNFLGLEQLEHLDFQHSNLKQMSEFSVFLSLRNLIYLDISHTHTRVAFNGIFNGLSSLEVLKMAGNSFQENFLPDIFTELRNLTFLDLSQCQLEQLSPTAFNSLSSLQVLNMSHNNFFSLDTFPYKCLNSLQVLDYSLNHIMTSKKQELQHFPSSLAFLNLTQNDFACTCEHQSFLQWIKDQRQLLVEVERMECATPSDKQGMPVLSLNITCQMNKTIIGVSVLSVLVVSVVAVLVYKFYFHLMLLAGCIKYGRGENIYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIHEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKVEKTLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLRKALLDGKSWNPEGTVGTGCNWQEATSI

Target ORF information:

RefSeq Version	NM_138557.3
Organism	Homo sapiens(human)
Definition	Homo sapiens toll like receptor 4 (TLR4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138557.3

ORF Insert Sequence:

ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA 
GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA 
AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG 
GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG 
TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT 
ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT 
GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT 
AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC 
AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC 
AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC 
CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC 
TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT 
TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA 
GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC 
AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC 
CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC 
AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT 
AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA 
AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC 
TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC 
TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG 
CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT 
GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT 
CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC 
TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG 
CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC 
AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC 
TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG 
AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG 
CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT 
GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA 
TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR4 ( NM_003266.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003257.1 Homo sapiens toll like receptor 4 (TLR4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu27264
Clone ID Related Accession (Same CDS sequence)	NM_003266.4 , NM_003266.3
Accession Version	NM_003266.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	toll-like receptor 4 isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA187988.1, BC143841.1, BC025294.1, AK226155.1, BG622357.1, DA218006.1 and AL160272.16. On Jun 1, 2019 this sequence version replaced NM_003266.3. Transcript Variant: This variant (3) contains an additional internal exon compared to transcript variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a weak Kozak sequence and a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG, which results in an isoform (C) with a shorter N-terminus compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.93093.1, SRR1803611.103081.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC 
CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG 
CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC 
CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA 
GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT 
CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT 
CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG 
GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA 
ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA 
GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA 
AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG 
GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG 
TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT 
ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT 
GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT 
AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC 
AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC 
AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC 
CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC 
TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT 
TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA 
GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC 
AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC 
CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC 
AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT 
AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA 
AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC 
TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC 
TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG 
CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT 
GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT 
CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC 
TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG 
CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC 
AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC 
TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG 
AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG 
CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT 
GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA 
TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003257.1
CDS	311..2710
Translation	MELNFYKIPDNLPFSTKNLDLSFNPLRHLGSYSFFSFPELQVLDLSRCEIQTIEDGAYQSLSHLSTLILTGNPIQSLALGAFSGLSSLQKLVAVETNLASLENFPIGHLKTLKELNVAHNLIQSFKLPEYFSNLTNLEHLDLSSNKIQSIYCTDLRVLHQMPLLNLSLDLSLNPMNFIQPGAFKEIRLHKLTLRNNFDSLNVMKTCIQGLAGLEVHRLVLGEFRNEGNLEKFDKSALEGLCNLTIEEFRLAYLDYYLDDIIDLFNCLTNVSSFSLVSVTIERVKDFSYNFGWQHLELVNCKFGQFPTLKLKSLKRLTFTSNKGGNAFSEVDLPSLEFLDLSRNGLSFKGCCSQSDFGTTSLKYLDLSFNGVITMSSNFLGLEQLEHLDFQHSNLKQMSEFSVFLSLRNLIYLDISHTHTRVAFNGIFNGLSSLEVLKMAGNSFQENFLPDIFTELRNLTFLDLSQCQLEQLSPTAFNSLSSLQVLNMSHNNFFSLDTFPYKCLNSLQVLDYSLNHIMTSKKQELQHFPSSLAFLNLTQNDFACTCEHQSFLQWIKDQRQLLVEVERMECATPSDKQGMPVLSLNITCQMNKTIIGVSVLSVLVVSVVAVLVYKFYFHLMLLAGCIKYGRGENIYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIHEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKVEKTLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLRKALLDGKSWNPEGTVGTGCNWQEATSI

Target ORF information:

RefSeq Version	NM_003266.4
Organism	Homo sapiens(human)
Definition	Homo sapiens toll like receptor 4 (TLR4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003266.4

ORF Insert Sequence:

ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC 
CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG 
CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC 
CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA 
GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT 
CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT 
CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG 
GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA 
ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA 
GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA 
AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG 
GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG 
TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT 
ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT 
GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT 
AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC 
AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC 
AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC 
CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC 
TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT 
TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA 
GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC 
AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC 
CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC 
AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT 
AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA 
AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC 
TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC 
TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG 
CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT 
GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT 
CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC 
TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG 
CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC 
AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC 
TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG 
AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG 
CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT 
GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA 
TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR4 ( NM_003266.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003257.1 Homo sapiens toll like receptor 4 (TLR4), transcript variant 3, mRNA.

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CloneID	OHu27264
Clone ID Related Accession (Same CDS sequence)	NM_003266.4 , NM_003266.3
Accession Version	NM_003266.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	toll-like receptor 4 isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA187988.1, BC143841.1, BC025294.1, AK226155.1, BG622357.1 and DA218006.1. On or before Jan 21, 2012 this sequence version replaced NR_024168.1, NM_003266.2. Transcript Variant: This variant (3) contains an additional internal exon compared to transcript variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a weak Kozak sequence and a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG, which results in an isoform (C) with a shorter N-terminus compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.103081.1, SRR1803612.123617.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC 
CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG 
CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC 
CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA 
GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT 
CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT 
CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG 
GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA 
ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA 
GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA 
AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG 
GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG 
TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT 
ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT 
GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT 
AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC 
AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC 
AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC 
CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC 
TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT 
TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA 
GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC 
AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC 
CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC 
AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT 
AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA 
AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC 
TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC 
TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG 
CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT 
GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT 
CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC 
TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG 
CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC 
AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC 
TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG 
AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG 
CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT 
GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA 
TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003257.1
CDS	539..2938
Translation	MELNFYKIPDNLPFSTKNLDLSFNPLRHLGSYSFFSFPELQVLDLSRCEIQTIEDGAYQSLSHLSTLILTGNPIQSLALGAFSGLSSLQKLVAVETNLASLENFPIGHLKTLKELNVAHNLIQSFKLPEYFSNLTNLEHLDLSSNKIQSIYCTDLRVLHQMPLLNLSLDLSLNPMNFIQPGAFKEIRLHKLTLRNNFDSLNVMKTCIQGLAGLEVHRLVLGEFRNEGNLEKFDKSALEGLCNLTIEEFRLAYLDYYLDDIIDLFNCLTNVSSFSLVSVTIERVKDFSYNFGWQHLELVNCKFGQFPTLKLKSLKRLTFTSNKGGNAFSEVDLPSLEFLDLSRNGLSFKGCCSQSDFGTTSLKYLDLSFNGVITMSSNFLGLEQLEHLDFQHSNLKQMSEFSVFLSLRNLIYLDISHTHTRVAFNGIFNGLSSLEVLKMAGNSFQENFLPDIFTELRNLTFLDLSQCQLEQLSPTAFNSLSSLQVLNMSHNNFFSLDTFPYKCLNSLQVLDYSLNHIMTSKKQELQHFPSSLAFLNLTQNDFACTCEHQSFLQWIKDQRQLLVEVERMECATPSDKQGMPVLSLNITCQMNKTIIGVSVLSVLVVSVVAVLVYKFYFHLMLLAGCIKYGRGENIYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIHEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKVEKTLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLRKALLDGKSWNPEGTVGTGCNWQEATSI

Target ORF information:

RefSeq Version	NM_003266.3
Organism	Homo sapiens(human)
Definition	Homo sapiens toll like receptor 4 (TLR4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003266.3

ORF Insert Sequence:

ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC 
CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG 
CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC 
CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA 
GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT 
CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT 
CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG 
GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA 
ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA 
GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA 
AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG 
GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG 
TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT 
ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT 
GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT 
AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC 
AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC 
AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC 
CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC 
TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT 
TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA 
GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC 
AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC 
CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC 
AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT 
AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA 
AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC 
TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC 
TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG 
CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT 
GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT 
CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC 
TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG 
CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC 
AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC 
TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG 
AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG 
CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT 
GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA 
TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TLR4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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