TLR4 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	TLR4
Entrez Gene ID	403417
Full Name	toll like receptor 4
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001002950.2	NP_001002950.2	toll-like receptor 4 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tlr4	NP_067272.1
Danio rerio (zebrafish)	tlr4ba	NP_001124523.1
Pan troglodytes (chimpanzee)	TLR4	NP_001138335.1
Gallus gallus (chicken)	TLR4	NP_001025864.1
Homo sapiens (human)	TLR4	NP_612564.1
	TLR4	NP_001032169.1
Rattus norvegicus (Norway rat)	Tlr4	NP_062051.1
Danio rerio (zebrafish)	tlr4al	XP_001919699.4
Danio rerio (zebrafish)	tlr4bb	NP_997978.1
Canis lupus familiaris (dog)	TLR4	NP_001002950.2
Bos taurus (cattle)	TLR4	NP_776623.5

Gene Ontology
Bibliography

Related articles in PubMed

Compartmentalized gene expression of toll-like receptors 2, 4 and 9 in the brain and peripheral lymphoid organs during canine visceral leishmaniasis.
Melo GD, Silva JE, Grano FG, Homem CG, Machado GF
Parasite immunology36(12)726-31(2014 Dec)

Assessment of Toll-like receptor 2, 4 and 9 SNP genotypes in canine sino-nasal aspergillosis.
Mercier E, Peters IR, Farnir F, Lavou? R, Day M, Clercx C, Peeters D
BMC veterinary research10187(2014 Aug)

Differential expression of Toll-like receptor 4 (TLR4) in healthy and infected canine endometrium.
Chotimanukul S, Sirivaidyapong S
Theriogenology76(6)1152-61(2011 Oct)

Polymorphisms in the TLR4 and TLR5 gene are significantly associated with inflammatory bowel disease in German shepherd dogs.
Kathrani A, House A, Catchpole B, Murphy A, German A, Werling D, Allenspach K
PloS one5(12)e15740(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study investigated the pattern of TLR-2, TLR-4 and TLR-9 gene expression in brain, choroid plexus, spleen and lymph node of dogs naturally infected with Leishmania infantum. In the brain, there was only an upregulation of TLR-2, in the choroid plexus, TLR-2 and TLR-9 were both upregulated and peripheral lymphoid organs showed increased TLR-2 and TLR-4 expression.
Title: Compartmentalized gene expression of toll-like receptors 2, 4 and 9 in the brain and peripheral lymphoid organs during canine visceral leishmaniasis.

These findings do not support a role for non-synonymous SNPs in the TLR 2, 4 and 9 coding regions in the pathogenesis of canine SNA, but do not exclude a role for innate immunity in the pathogenesis of the disease.
Title: Assessment of Toll-like receptor 2, 4 and 9 SNP genotypes in canine sino-nasal aspergillosis.

TLR4 is expressed in the surface epithelium, glandular epithelium and stroma throughout the estrous cycle and in pyometra.
Title: Differential expression of Toll-like receptor 4 (TLR4) in healthy and infected canine endometrium.

TLR4 and TLR5 polymorphisms are significantly associated with inflammatory bowel disease in German shepherd dogs
Title: Polymorphisms in the TLR4 and TLR5 gene are significantly associated with inflammatory bowel disease in German shepherd dogs.

Increased TLR-4 gene expression in the synovial tissue of stifle jointss in dogs with osteoarthritis.
Title: Expression of Toll-like receptors 2 and 4 in stifle joint synovial tissues of dogs with or without osteoarthritis.

The following TLR4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLR4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe33621	NM_001002950.2 Latest version!	Canis lupus familiaris toll like receptor 4 (TLR4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLR4 ( NM_001002950.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001002950.2 Canis lupus familiaris toll like receptor 4 (TLR4), mRNA.

Price & Availability↑

CloneID	OCe33621
Clone ID Related Accession (Same CDS sequence)	NM_001002950.2
Accession Version	NM_001002950.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Canis lupus familiaris(dog)
Product	toll-like receptor 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03008139.1 and AB080363.1. On Oct 6, 2010 this sequence version replaced NM_001002950.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignment to orthologus human transcript (NM_138554.3). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGTCTC CTACCCGCCT GGTTGGGATA TTGATCCCAG CCATGGCCTT CCTCTCCTGC 
CTGAGACCTG AGAGCTGGGA CCCTTGCATG CAGGTGGTTG CTAACACTAC TTACCAATGC 
ATGGAGCTGA ATCTCAGCAA AATCCCCAAC AACATCCCCA CATCAACCGA GAAACTGGAC 
CTGAGCTTTA ACCCCCTGAG GCATTTAGGC AGCCATTGCT TCTCCAACTT CCCAAAACTG 
CAGGTGCTGG ATTTATCCAG GTGTGAAATT CAGGTAATTG AAGATGATGC ATATCAGGGC 
CTAAACCACC TCTCCATCTT GATATTGACA GGAAACCCTA TCCAGAGATT ATTTCCAAGA 
GCCTTTTCTG GACTATCAAG TTTAAAGACG CTGGTGGCCA AGGAGACAAA ACTAACATCT 
CTAGAGGATT TCCCCATTGG ACATCTCAAA ACCTTGAAGG AGCTTAATGT GGCTCACAAT 
CTTATCCATT CCTTCAAGCT ACCTGCGTAT TTCTCTAACA TGCCCAACCT GGAGAACGTG 
GATCTTTCCA ATAACAAGAT CCAAAATATT TATCGTGAAG ACTTGCAGGT TCTACATCAC 
ATGCCACTAC TCAACCTTTC TTTAGACTTG TCCCTGAACC CTTTATACTT TATCCAACCA 
GGTTCCTTTA AAGAAATTAA ACTCCATAAA CTGACTTTGA GAAGTAATTT TAATAGTACA 
GATGTAATGA AAACTTTTAT TCAAGGTCTG GCTGGCTTAA AGATCAATCA GTTGGTTCTG 
GGAGAATTTA AAAATGAAAG GAAGTTGGAA AGCTTTGACA ATTCTCTCCT GGAAGGACTG 
TGCAATTTGA CCATTGAAAA ATTCCGGATA GCATACTTTG ATAGCTTCTC AAAGGATACT 
ACTAACTTAT TTAACCAGTT GGTAAACATT TCTGCAATTT CTTTGGCGCA TCTGTATTTA 
GACACACCAA AATACCTTCC TAAAAATCTC AGATGGCAAC GGTTGGAAAT AGTTAATTGT 
AACTTAGAAC AATTTCCCGC ATGGGAGCTG GACTCTCTCA AGGAGTTTGT TCTCACTTCC 
AACAAAGGTA TGAACACTTT TGCTGATATG AAGATGGAAA GCCTTGAGTT TCTAGATCTC 
AGTAGAAATC GCCTGAGTTT CAAGACTTGC TGCTCTCACT CTGATTTTGG GACAACCAGA 
CTGAAGCATT TAGATCTGAG CTTCAATGAA ATCATTACCA TGAGTTCAAA CTTCTTGGGC 
TTAGAACAAC TGGAATATCT AGATTTACAG CATTCCAGTT TGAAGCAGGC CAGTGATTTT 
TCAGTATTCT TGTCCCTCAG AAACCTCCGT TACCTTGATA TTTCTTATAC TCGCACTGAA 
GTTGCTTTCC AGGGCATTTT TGATGGCTTG GTCAGCCTCG AAGTCTTGAA AATGGCTGAT 
AATTCCTTTC CGGACAACTC CCTTCCAAAT ATTTTCAAAG GGTTGACTAA CTTAACCATT 
CTGGACCTTT CTAGGTGTCA TCTGGAACGG GTGTCCCAGG AATCATTTGT CTCACTTCCT 
AAACTTCAGG AGATAAATAT GAGTCACAAT AGCCTCTTGT CATTGGATAC ACTAGCTTAT 
GAACCTCTCC TCTCCCTCCA GATCCTAGAT TGCAGTTTCA ATCGAATAGT AGCCTTCAAG 
GAACAAGGAC AACAGCATTT TCCAAGTAAT CTAGTTTCCT TAAATCTTAC TCGGAATAAC 
TTTGCTTGTG ACTGTGAACA TCAGAGTTTC CTGCAGTGGG TCAAAGACCA CAGACAGCTC 
TTGGTGGAAG TTGAACAAAT GGTGTGTGCA AAACCTTTAG ACATGAAGGA CATGCCCTTG 
CTAAGTTTTA GGAATGCCAC CTGTCAGAGG AGCAAGACTA TCATTAGTGT GTCAGTTTTC 
ACTGTGCTCA TGGTTTCTCT GGTAGCAGTT TTAGCGTATA AGTTCTATTT TCACCTGATG 
CTTCTCGCTG GCTGCAAAAG GTATAACAGA GGGGAAAGTA CCTATGATGC ATTTGTTATC 
TACTCAAGCC AGGATGAAGA CTGGGTGAGG AATGAATTGG TAAAGAACTT GGAGGAGGGA 
GTGCCCCCCT TTCAGCTCTG CCTTCACTAC AGAGACTTCA TTCCTGGTGT GGCCATCGCC 
GCCAACATCA TCCAGGAAGG CTTCTACAAA AGCCGGAAGG TTATTGTTGT GGTGTCCCAA 
CACTTCATCC AGAGTCGATG GTGCATCTTT GAGTATGAGA TTGCCCAGAC TTGGCAGTTT 
CTTAGCAGTC GTGCTGGCAT CATCTTCATT GTCCTGCAGA AGGTGGAGAA GTCCCTGCTG 
CGGCAGCAGG TGGAACTGTA TCGCCTCCTC AGCAGGAACA CTTACCTGGA ATGGGAAGAC 
AGTGTCCTGG GGCGGCACAT CTTCTGGAGA CGGCTCCGAA AAGCCTTGCT GGATGGTAAA 
CCGTGGAGTC CAGAAGGAAC AGAGGATGCA GAAAAAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002950.2
CDS	9..2510
Translation	MMSPTRLVGILIPAMAFLSCLRPESWDPCMQVVANTTYQCMELNLSKIPNNIPTSTEKLDLSFNPLRHLGSHCFSNFPKLQVLDLSRCEIQVIEDDAYQGLNHLSILILTGNPIQRLFPRAFSGLSSLKTLVAKETKLTSLEDFPIGHLKTLKELNVAHNLIHSFKLPAYFSNMPNLENVDLSNNKIQNIYREDLQVLHHMPLLNLSLDLSLNPLYFIQPGSFKEIKLHKLTLRSNFNSTDVMKTFIQGLAGLKINQLVLGEFKNERKLESFDNSLLEGLCNLTIEKFRIAYFDSFSKDTTNLFNQLVNISAISLAHLYLDTPKYLPKNLRWQRLEIVNCNLEQFPAWELDSLKEFVLTSNKGMNTFADMKMESLEFLDLSRNRLSFKTCCSHSDFGTTRLKHLDLSFNEIITMSSNFLGLEQLEYLDLQHSSLKQASDFSVFLSLRNLRYLDISYTRTEVAFQGIFDGLVSLEVLKMADNSFPDNSLPNIFKGLTNLTILDLSRCHLERVSQESFVSLPKLQEINMSHNSLLSLDTLAYEPLLSLQILDCSFNRIVAFKEQGQQHFPSNLVSLNLTRNNFACDCEHQSFLQWVKDHRQLLVEVEQMVCAKPLDMKDMPLLSFRNATCQRSKTIISVSVFTVLMVSLVAVLAYKFYFHLMLLAGCKRYNRGESTYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIQEGFYKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKVEKSLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLRKALLDGKPWSPEGTEDAEKS

Target ORF information:

RefSeq Version	NM_001002950.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris toll like receptor 4 (TLR4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002950.2

ORF Insert Sequence:

ATGATGTCTC CTACCCGCCT GGTTGGGATA TTGATCCCAG CCATGGCCTT CCTCTCCTGC 
CTGAGACCTG AGAGCTGGGA CCCTTGCATG CAGGTGGTTG CTAACACTAC TTACCAATGC 
ATGGAGCTGA ATCTCAGCAA AATCCCCAAC AACATCCCCA CATCAACCGA GAAACTGGAC 
CTGAGCTTTA ACCCCCTGAG GCATTTAGGC AGCCATTGCT TCTCCAACTT CCCAAAACTG 
CAGGTGCTGG ATTTATCCAG GTGTGAAATT CAGGTAATTG AAGATGATGC ATATCAGGGC 
CTAAACCACC TCTCCATCTT GATATTGACA GGAAACCCTA TCCAGAGATT ATTTCCAAGA 
GCCTTTTCTG GACTATCAAG TTTAAAGACG CTGGTGGCCA AGGAGACAAA ACTAACATCT 
CTAGAGGATT TCCCCATTGG ACATCTCAAA ACCTTGAAGG AGCTTAATGT GGCTCACAAT 
CTTATCCATT CCTTCAAGCT ACCTGCGTAT TTCTCTAACA TGCCCAACCT GGAGAACGTG 
GATCTTTCCA ATAACAAGAT CCAAAATATT TATCGTGAAG ACTTGCAGGT TCTACATCAC 
ATGCCACTAC TCAACCTTTC TTTAGACTTG TCCCTGAACC CTTTATACTT TATCCAACCA 
GGTTCCTTTA AAGAAATTAA ACTCCATAAA CTGACTTTGA GAAGTAATTT TAATAGTACA 
GATGTAATGA AAACTTTTAT TCAAGGTCTG GCTGGCTTAA AGATCAATCA GTTGGTTCTG 
GGAGAATTTA AAAATGAAAG GAAGTTGGAA AGCTTTGACA ATTCTCTCCT GGAAGGACTG 
TGCAATTTGA CCATTGAAAA ATTCCGGATA GCATACTTTG ATAGCTTCTC AAAGGATACT 
ACTAACTTAT TTAACCAGTT GGTAAACATT TCTGCAATTT CTTTGGCGCA TCTGTATTTA 
GACACACCAA AATACCTTCC TAAAAATCTC AGATGGCAAC GGTTGGAAAT AGTTAATTGT 
AACTTAGAAC AATTTCCCGC ATGGGAGCTG GACTCTCTCA AGGAGTTTGT TCTCACTTCC 
AACAAAGGTA TGAACACTTT TGCTGATATG AAGATGGAAA GCCTTGAGTT TCTAGATCTC 
AGTAGAAATC GCCTGAGTTT CAAGACTTGC TGCTCTCACT CTGATTTTGG GACAACCAGA 
CTGAAGCATT TAGATCTGAG CTTCAATGAA ATCATTACCA TGAGTTCAAA CTTCTTGGGC 
TTAGAACAAC TGGAATATCT AGATTTACAG CATTCCAGTT TGAAGCAGGC CAGTGATTTT 
TCAGTATTCT TGTCCCTCAG AAACCTCCGT TACCTTGATA TTTCTTATAC TCGCACTGAA 
GTTGCTTTCC AGGGCATTTT TGATGGCTTG GTCAGCCTCG AAGTCTTGAA AATGGCTGAT 
AATTCCTTTC CGGACAACTC CCTTCCAAAT ATTTTCAAAG GGTTGACTAA CTTAACCATT 
CTGGACCTTT CTAGGTGTCA TCTGGAACGG GTGTCCCAGG AATCATTTGT CTCACTTCCT 
AAACTTCAGG AGATAAATAT GAGTCACAAT AGCCTCTTGT CATTGGATAC ACTAGCTTAT 
GAACCTCTCC TCTCCCTCCA GATCCTAGAT TGCAGTTTCA ATCGAATAGT AGCCTTCAAG 
GAACAAGGAC AACAGCATTT TCCAAGTAAT CTAGTTTCCT TAAATCTTAC TCGGAATAAC 
TTTGCTTGTG ACTGTGAACA TCAGAGTTTC CTGCAGTGGG TCAAAGACCA CAGACAGCTC 
TTGGTGGAAG TTGAACAAAT GGTGTGTGCA AAACCTTTAG ACATGAAGGA CATGCCCTTG 
CTAAGTTTTA GGAATGCCAC CTGTCAGAGG AGCAAGACTA TCATTAGTGT GTCAGTTTTC 
ACTGTGCTCA TGGTTTCTCT GGTAGCAGTT TTAGCGTATA AGTTCTATTT TCACCTGATG 
CTTCTCGCTG GCTGCAAAAG GTATAACAGA GGGGAAAGTA CCTATGATGC ATTTGTTATC 
TACTCAAGCC AGGATGAAGA CTGGGTGAGG AATGAATTGG TAAAGAACTT GGAGGAGGGA 
GTGCCCCCCT TTCAGCTCTG CCTTCACTAC AGAGACTTCA TTCCTGGTGT GGCCATCGCC 
GCCAACATCA TCCAGGAAGG CTTCTACAAA AGCCGGAAGG TTATTGTTGT GGTGTCCCAA 
CACTTCATCC AGAGTCGATG GTGCATCTTT GAGTATGAGA TTGCCCAGAC TTGGCAGTTT 
CTTAGCAGTC GTGCTGGCAT CATCTTCATT GTCCTGCAGA AGGTGGAGAA GTCCCTGCTG 
CGGCAGCAGG TGGAACTGTA TCGCCTCCTC AGCAGGAACA CTTACCTGGA ATGGGAAGAC 
AGTGTCCTGG GGCGGCACAT CTTCTGGAGA CGGCTCCGAA AAGCCTTGCT GGATGGTAAA 
CCGTGGAGTC CAGAAGGAAC AGAGGATGCA GAAAAAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Compartmentalized gene expression of toll-like receptors 2, 4 and 9 in the brain and peripheral lymphoid organs during canine visceral leishmaniasis.
Parasite immunology36(12)726-31(2014 Dec)
Melo GD,Silva JE,Grano FG,Homem CG,Machado GF

Assessment of Toll-like receptor 2, 4 and 9 SNP genotypes in canine sino-nasal aspergillosis.
BMC veterinary research10187(2014 Aug)
Mercier E,Peters IR,Farnir F,Lavou? R,Day M,Clercx C,Peeters D

Differential expression of Toll-like receptor 4 (TLR4) in healthy and infected canine endometrium.
Theriogenology76(6)1152-61(2011 Oct)
Chotimanukul S,Sirivaidyapong S

Polymorphisms in the TLR4 and TLR5 gene are significantly associated with inflammatory bowel disease in German shepherd dogs.
PloS one5(12)e15740(2010 Dec)
Kathrani A,House A,Catchpole B,Murphy A,German A,Werling D,Allenspach K

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TLR4 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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