TLR4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TLR4
Entrez Gene ID	281536
Full Name	toll like receptor 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005210586.3	XP_005210643.1	toll-like receptor 4 isoform X1
XM_005210586.4	XP_005210643.1	toll-like receptor 4 isoform X2
NM_174198.6	NP_776623.5	toll-like receptor 4 precursor
XM_024995446.1	XP_024851214.1	toll-like receptor 4 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tlr4	NP_067272.1
Danio rerio (zebrafish)	tlr4ba	NP_001124523.1
Pan troglodytes (chimpanzee)	TLR4	NP_001138335.1
Gallus gallus (chicken)	TLR4	NP_001025864.1
Homo sapiens (human)	TLR4	NP_612564.1
	TLR4	NP_001032169.1
Rattus norvegicus (Norway rat)	Tlr4	NP_062051.1
Danio rerio (zebrafish)	tlr4al	XP_001919699.4
Danio rerio (zebrafish)	tlr4bb	NP_997978.1
Canis lupus familiaris (dog)	TLR4	NP_001002950.2
Bos taurus (cattle)	TLR4	NP_776623.5

Gene Ontology
Bibliography

Related articles in PubMed

Dominance effects estimation of TLR4 and CACNA2D1 genes for health and production traits using logistic regression.
Bagheri M, Zahmatkesh A
Journal of genetics96(6)1027-1031(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studied genetic diversity of the Toll-like receptor gene TLR4 in Czech Red and Czech Red Pied cattle. Found 8 SNPs, which were grouped into 18 haplotypes.
Title: Diversity of the TLR4 Immunity Receptor in Czech Native Cattle Breeds Revealed Using the Pacific Biosciences Sequencing Platform.

TLR4 polymorphisms are associated with lower reproductive Performance.
Title: Effect of Single Nucleotide Polymorphisms of Toll-Like Receptor 4 (TLR 4) on Reproductive Performance and Immune Function in Dairy Cows.

As a pilot study, the present results revealed that identified SNPs in IL8 and TLR4 genes can be used as a genetic marker and predisposing factor for resistance/susceptibility to digital dermatitis in dairy cows. However, TLR4 gene may be a potential candidate for such disease.
Title: Single Nucleotide Polymorphisms in IL8 and TLR4 Genes as Candidates for Digital Dermatitis Resistance/Susceptibility in Holstein Cattle.

STA3 facilitates TLR4-dependent IL-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells.
Title: Signal transducer and activator of transcription-3 licenses Toll-like receptor 4-dependent interleukin (IL)-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells.

Transcription levels of TLR2, TLR4, and CD14 in Holstein cows with retained placenta significantly decreased between the first and the seventh day postpartum.
Title: Changes in endometrial transcription of TLR2, TLR4, and CD14 during the first-week postpartum in dairy cows with retained placenta.

The following TLR4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLR4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa22402	XM_005210586.3 Latest version!	Bos taurus toll like receptor 4 (TLR4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa01214	NM_174198.6 Latest version!	Bos taurus toll like receptor 4 (TLR4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa236926	XM_024995446.1 Latest version!	Bos taurus toll like receptor 4 (TLR4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa236927	XM_005210586.4 Latest version!	Bos taurus toll like receptor 4 (TLR4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLR4 ( XM_005210586.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210643.1 Bos taurus toll like receptor 4 (TLR4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa22402
Clone ID Related Accession (Same CDS sequence)	XM_005210586.3
Accession Version	XM_005210586.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	toll-like receptor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104150.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210586.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTAC TCAACCTCTC TTTAGATTTG TCCTTGAACC CTTTAGACTT TATTGAACCA 
GGTACCTTTA AAGAAATTAA GCTCAATGGA TTGACTCTGC GAAGTAATTT TAACAGTTCA 
CATGTCATGA AAACCTGTAT TCAAGGTCTG GCTGGTTTAA AAACCAACCG GCTGGTTTTG 
GGAGAATTTA AAAATGAAAG GAAGTTGCAA AGATTTGACA GATCTTTCCT GGAGGGACTG 
TGCAACCTGA CCATTGAACA ATTCCGGATA GCGTACTTGG ACAAATTCTC AGGGGACGAT 
ACAGACTTAT TTAATTGTTT GGCAAATGTT TCTGTGATTT CTCTGTTGAG TATATCTTTA 
GGAAGTCTAC AAGCCCTTCT TAAAGATTTT AGATGGCAAC ACTTAGAAAT TATTAACTGT 
GACTTTGACA AGTTTCCTGC ATTGAAGCTC AGTTCTCTCA AAAAGTTTGT TTTCACAGAC 
AACAAAGATA TAAGCACTTT TACTGAGTTT CAGCTACCAA GCCTTCAGTA TCTAGATCTC 
AAAAGAAATC ACTTGAGTTT CAAGGGTTGC TGTTCTCACA CTGATTTTGG GACAACCAAC 
CTGAAGCATT TAGATCTGAG CTTCAATGAT GTCATTACCT TAGGTTCAAA CTTCATGGGC 
TTAGAGCAAC TAGAACACCT GGATTTTCAG CATTCCACTC TGAAACAGAT CAATGCTTTT 
TCAGCATTCC TATCACTCAG AAACCTCCGC TACCTTGATA TTTCTTATAC CAACATCCGC 
ATTGTCTTCC ATGGCATCTT TACTGGCTTA GTCAGTCTGC AAACCTTGAA AATGGCAGGC 
AACTCTTTTC AGAACAACTT GCTCCCTGAC ATCTTCACAG AACTGACTAA CTTAACCGTC 
TTGGACCTCT CTAAGTGTCA ACTGGAACAG GTAGCCCAGA CAGCATTTCA CTCCCTCTCT 
AGCCTTCAGG TGCTGAATAT GAGTCACAAC AAACTCTTGT CACTGGATAC ATTTCTTTAT 
GAACCACTCC ACTCGCTCCG GATCCTAGAC TGCAGCTTCA ACCGTATCAT GGCCTCTAAG 
GAGCAAGAAC TACAGAATTT GCCAAGGAGC CTCACTTGGC TAAATCTTAC TCAGAATGCA 
TTTGCTTGTG TTTGTGAACA TCAGAGTTTC CTGCAGTGGG TCAAGGACCA GAGGCAGCTC 
TTGGTGGGAG CTGAGCAAAT GATGTGTGCA GAGCCTTTAG ATATGGAGGA CATGCCAGTG 
CTTAGTTTCA GGAACGCCAC TTGTCAGCTG AGCAAGACGA TCATCAGTGT GTCGGTGGTC 
ACTGTGCTCC TGGTGTCTGT GGTAGGGGTC CTAGTCTACA AGTTCTATTT CCACCTGATG 
CTTCTTGCTG GCTGCAAAAA GTATGGCAGG GGCGAGAGCA CCTATGATGC CTTTGTCATC 
TACTCGAGCC AGGATGAAGA CTGGGTGCGG AATGAACTGG TAAAGAACTT GGAGGAGGGC 
GTGCCCCCCT TTCAGCTCTG CCTTCACTAC AGGGACTTTA TTCCTGGGGT GGCCATCGCC 
GCCAATATCA TCCAGGAGGG TTTCCACAAA AGCCGTAAGG TTATTGTCGT GGTGTCCCAG 
CACTTCATCC AGAGCCGATG GTGTATCTTC GAGTATGAGA TTGCCCAGAC CTGGCAGTTT 
CTGAGCAGCC GTGCTGGCAT CATCTTCATC GTCCTGCAGA AGCTGGAGAA GTCTCTCCTG 
CGGCAGCAGG TGGAGCTCTA TCGCCTTCTG AGCAGGAACA CCTACCTGGA GTGGGAGGAC 
AGTGTCCTGG GGCGGCATGT CTTCTGGAGA AGACTCAGAA AAGCCTTGCT GGCTGGTAAA 
CCCCAGAGTC CAGAAGGAAC AGCAGATGCA GAAACCAACC CGCAAGAAGC GACAACCTCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210643.1
CDS	723..2648
Translation	MPLLNLSLDLSLNPLDFIEPGTFKEIKLNGLTLRSNFNSSHVMKTCIQGLAGLKTNRLVLGEFKNERKLQRFDRSFLEGLCNLTIEQFRIAYLDKFSGDDTDLFNCLANVSVISLLSISLGSLQALLKDFRWQHLEIINCDFDKFPALKLSSLKKFVFTDNKDISTFTEFQLPSLQYLDLKRNHLSFKGCCSHTDFGTTNLKHLDLSFNDVITLGSNFMGLEQLEHLDFQHSTLKQINAFSAFLSLRNLRYLDISYTNIRIVFHGIFTGLVSLQTLKMAGNSFQNNLLPDIFTELTNLTVLDLSKCQLEQVAQTAFHSLSSLQVLNMSHNKLLSLDTFLYEPLHSLRILDCSFNRIMASKEQELQNLPRSLTWLNLTQNAFACVCEHQSFLQWVKDQRQLLVGAEQMMCAEPLDMEDMPVLSFRNATCQLSKTIISVSVVTVLLVSVVGVLVYKFYFHLMLLAGCKKYGRGESTYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIQEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKLEKSLLRQQVELYRLLSRNTYLEWEDSVLGRHVFWRRLRKALLAGKPQSPEGTADAETNPQEATTST

Target ORF information:

RefSeq Version	XM_005210586.3
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 4 (TLR4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210586.3

ORF Insert Sequence:

ATGCCCCTAC TCAACCTCTC TTTAGATTTG TCCTTGAACC CTTTAGACTT TATTGAACCA 
GGTACCTTTA AAGAAATTAA GCTCAATGGA TTGACTCTGC GAAGTAATTT TAACAGTTCA 
CATGTCATGA AAACCTGTAT TCAAGGTCTG GCTGGTTTAA AAACCAACCG GCTGGTTTTG 
GGAGAATTTA AAAATGAAAG GAAGTTGCAA AGATTTGACA GATCTTTCCT GGAGGGACTG 
TGCAACCTGA CCATTGAACA ATTCCGGATA GCGTACTTGG ACAAATTCTC AGGGGACGAT 
ACAGACTTAT TTAATTGTTT GGCAAATGTT TCTGTGATTT CTCTGTTGAG TATATCTTTA 
GGAAGTCTAC AAGCCCTTCT TAAAGATTTT AGATGGCAAC ACTTAGAAAT TATTAACTGT 
GACTTTGACA AGTTTCCTGC ATTGAAGCTC AGTTCTCTCA AAAAGTTTGT TTTCACAGAC 
AACAAAGATA TAAGCACTTT TACTGAGTTT CAGCTACCAA GCCTTCAGTA TCTAGATCTC 
AAAAGAAATC ACTTGAGTTT CAAGGGTTGC TGTTCTCACA CTGATTTTGG GACAACCAAC 
CTGAAGCATT TAGATCTGAG CTTCAATGAT GTCATTACCT TAGGTTCAAA CTTCATGGGC 
TTAGAGCAAC TAGAACACCT GGATTTTCAG CATTCCACTC TGAAACAGAT CAATGCTTTT 
TCAGCATTCC TATCACTCAG AAACCTCCGC TACCTTGATA TTTCTTATAC CAACATCCGC 
ATTGTCTTCC ATGGCATCTT TACTGGCTTA GTCAGTCTGC AAACCTTGAA AATGGCAGGC 
AACTCTTTTC AGAACAACTT GCTCCCTGAC ATCTTCACAG AACTGACTAA CTTAACCGTC 
TTGGACCTCT CTAAGTGTCA ACTGGAACAG GTAGCCCAGA CAGCATTTCA CTCCCTCTCT 
AGCCTTCAGG TGCTGAATAT GAGTCACAAC AAACTCTTGT CACTGGATAC ATTTCTTTAT 
GAACCACTCC ACTCGCTCCG GATCCTAGAC TGCAGCTTCA ACCGTATCAT GGCCTCTAAG 
GAGCAAGAAC TACAGAATTT GCCAAGGAGC CTCACTTGGC TAAATCTTAC TCAGAATGCA 
TTTGCTTGTG TTTGTGAACA TCAGAGTTTC CTGCAGTGGG TCAAGGACCA GAGGCAGCTC 
TTGGTGGGAG CTGAGCAAAT GATGTGTGCA GAGCCTTTAG ATATGGAGGA CATGCCAGTG 
CTTAGTTTCA GGAACGCCAC TTGTCAGCTG AGCAAGACGA TCATCAGTGT GTCGGTGGTC 
ACTGTGCTCC TGGTGTCTGT GGTAGGGGTC CTAGTCTACA AGTTCTATTT CCACCTGATG 
CTTCTTGCTG GCTGCAAAAA GTATGGCAGG GGCGAGAGCA CCTATGATGC CTTTGTCATC 
TACTCGAGCC AGGATGAAGA CTGGGTGCGG AATGAACTGG TAAAGAACTT GGAGGAGGGC 
GTGCCCCCCT TTCAGCTCTG CCTTCACTAC AGGGACTTTA TTCCTGGGGT GGCCATCGCC 
GCCAATATCA TCCAGGAGGG TTTCCACAAA AGCCGTAAGG TTATTGTCGT GGTGTCCCAG 
CACTTCATCC AGAGCCGATG GTGTATCTTC GAGTATGAGA TTGCCCAGAC CTGGCAGTTT 
CTGAGCAGCC GTGCTGGCAT CATCTTCATC GTCCTGCAGA AGCTGGAGAA GTCTCTCCTG 
CGGCAGCAGG TGGAGCTCTA TCGCCTTCTG AGCAGGAACA CCTACCTGGA GTGGGAGGAC 
AGTGTCCTGG GGCGGCATGT CTTCTGGAGA AGACTCAGAA AAGCCTTGCT GGCTGGTAAA 
CCCCAGAGTC CAGAAGGAAC AGCAGATGCA GAAACCAACC CGCAAGAAGC GACAACCTCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR4 ( NM_174198.6 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776623.5 Bos taurus toll like receptor 4 (TLR4), mRNA.

Price & Availability↑

CloneID	OBa01214
Clone ID Related Accession (Same CDS sequence)	NM_174198.6
Accession Version	NM_174198.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	toll-like receptor 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ839566.1. On Mar 8, 2007 this sequence version replaced NM_174198.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ839566.1, AY297040.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGATGGCGC GTGCCCGCCT GGCTGCGGCT CTGATCCCAG CCACGGCCAT CCTCTCCTGC 
CTGAGAACCG AGAGTTGGGA CCCTTGCGTA CAGGTTGTTC CTAACATTAG TTACCAGTGC 
ATGGAGCTGA ATCTCTACAA AATCCCCGAC AACATCCCCA TATCAACCAA GATGCTGGAC 
CTGAGCTTTA ACTACCTGAG ACATTTAGGC AGCCATAACT TCTCCAGCTT CCCAGAACTG 
CAAGTGCTGG ATTTATCCAG ATGTGAAATT AAGATTATTG AAGACGACAC ATTTCAGGGC 
CTAAACCACC TCTCCACCTT GATACTGACG GGAAACCCTA TCCAGAGTTT AGCCTGGGGA 
GCCTTTTCTG GGCTATCAAG TTTACAGAAG CTGGTGGCCG TGGAGACAAA CCTAGTCTCT 
CTAAATGACT TCCCCATTGG ACATCTCAAA AACTTGAAAG AGCTTAATGT GGCTCACAAT 
TTTATCCATT CCTTCAAGTT ACCTGAATAT TTTTCTAACC TGCCCAACCT GGAGCACTTG 
GATCTTTCTA ACAACAAAAT CCAAAATATT TATTATGAAG ATGTGAAAGT TCTACATCAA 
ATGCCCCTAC TCAACCTCTC TTTAGATTTG TCCTTGAACC CTTTAGACTT TATTGAACCA 
GGTACCTTTA AAGAAATTAA GCTCAATGGA TTGACTCTGC GAAGTAATTT TAACAGTTCA 
CATGTCATGA AAACCTGTAT TCAAGGTCTG GCTGGTTTAA AAACCAACCG GCTGGTTTTG 
GGAGAATTTA AAAATGAAAG GAAGTTGCAA AGATTTGACA GATCTTTCCT GGAGGGACTG 
TGCAACCTGA CCATTGAACA ATTCCGGATA GCGTACTTGG ACAAATTCTC AGGGGACGAT 
ACAGACTTAT TTAATTGTTT GGCAAATGTT TCTGTGATTT CTCTGTTGAG TATATCTTTA 
GGAAGTCTAC AAGCCCTTCT TAAAGATTTT AGATGGCAAC ACTTAGAAAT TATTAACTGT 
GACTTTGACA AGTTTCCTGC ATTGAAGCTC AGTTCTCTCA AAAAGTTTGT TTTCACAGAC 
AACAAAGATA TAAGCACTTT TACTGAGTTT CAGCTACCAA GCCTTCAGTA TCTAGATCTC 
AAAAGAAATC ACTTGAGTTT CAAGGGTTGC TGTTCTCACA CTGATTTTGG GACAACCAAC 
CTGAAGCATT TAGATCTGAG CTTCAATGAT GTCATTACCT TAGGTTCAAA CTTCATGGGC 
TTAGAGCAAC TAGAACACCT GGATTTTCAG CATTCCACTC TGAAACAGAT CAATGCTTTT 
TCAGCATTCC TATCACTCAG AAACCTCCGC TACCTTGATA TTTCTTATAC CAACATCCGC 
ATTGTCTTCC ATGGCATCTT TACTGGCTTA GTCAGTCTGC AAACCTTGAA AATGGCAGGC 
AACTCTTTTC AGAACAACTT GCTCCCTGAC ATCTTCACAG AACTGACTAA CTTAACCGTC 
TTGGACCTCT CTAAGTGTCA ACTGGAACAG GTAGCCCAGA CAGCATTTCA CTCCCTCTCT 
AGCCTTCAGG TGCTGAATAT GAGTCACAAC AAACTCTTGT CACTGGATAC ATTTCTTTAT 
GAACCACTCC ACTCGCTCCG GATCCTAGAC TGCAGYTTCA ACCGTATCAT GGCCTCTAAG 
GAGCAAGAAC TACAGAATTT GCCAAGGAGC CTCACTTGGC TAAATCTTAC TCAGAATGCA 
TTTGCTTGTG TTTGTGAACA TCAGAGTTTC CTGCAGTGGG TCAAGGACCA GAGGCAGCTC 
TTGGTGGGAG CTGAGCAAAT GATGTGTGCA GAGCCTTTAG ATATGGAGGA CATGCCAGTG 
CTTAGTTTCA GGAACGCCAC TTGTCAGCTG AGCAAGACGA TCATCAGTGT GTCGGTGGTC 
ACTGTGCTCC TGGTGTCTGT GGTAGGGGTC CTAGTCTACA AGTTCTATTT CCACCTGATG 
CTTCTTGCTG GCTGCAAAAA GTATGGCAGG GGCGAGAGCA YCTATGATGC CTTTGTCATC 
TACTCGAGCC AGGATGAAGA CTGGGTGCGG AATGAACTGG TAAAGAACTT GGAGGAGGGC 
GTGCCCCCCT TTCAGCTCTG CCTTCACTAC AGGGACTTTA TTCCTGGGGT GGCCATCGCC 
GCCAATATCA TCCAGGAGGG TTTCCACAAA AGCCGTAAGG TTATTGTCGT GGTGTCCCAG 
CACTTCATCC AGAGCCGATG GTGTATCTTC GAGTATGAGA TTGCCCAGAC CTGGCAGTTT 
CTGAGCAGCC GTGCTGGCAT CATCTTCATC GTCCTGCAGA AGCTGGAGAA GTCTCTCCTG 
CGGCAGCAGG TGGAGCTCTA TCGCCTTCTG AGCAGGAACA CCTACCTGGA GTGGGAGGAC 
AGTGTCCTGG GGCGGCATGT CTTCTGGAGA AGACTCAGAA AAGCCTTGCT GGCTGGTAAA 
CCCCAGAGTC CAGAAGGAAC AGCAGATGCA GAAACCAACC CGCAAGAAGC GACAACCTCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776623.5
CDS	471..2996
Translation	MMARARLAAALIPATAILSCLRTESWDPCVQVVPNISYQCMELNLYKIPDNIPISTKMLDLSFNYLRHLGSHNFSSFPELQVLDLSRCEIKIIEDDTFQGLNHLSTLILTGNPIQSLAWGAFSGLSSLQKLVAVETNLVSLNDFPIGHLKNLKELNVAHNFIHSFKLPEYFSNLPNLEHLDLSNNKIQNIYYEDVKVLHQMPLLNLSLDLSLNPLDFIEPGTFKEIKLNGLTLRSNFNSSHVMKTCIQGLAGLKTNRLVLGEFKNERKLQRFDRSFLEGLCNLTIEQFRIAYLDKFSGDDTDLFNCLANVSVISLLSISLGSLQALLKDFRWQHLEIINCDFDKFPALKLSSLKKFVFTDNKDISTFTEFQLPSLQYLDLKRNHLSFKGCCSHTDFGTTNLKHLDLSFNDVITLGSNFMGLEQLEHLDFQHSTLKQINAFSAFLSLRNLRYLDISYTNIRIVFHGIFTGLVSLQTLKMAGNSFQNNLLPDIFTELTNLTVLDLSKCQLEQVAQTAFHSLSSLQVLNMSHNKLLSLDTFLYEPLHSLRILDCnullFNRIMASKEQELQNLPRSLTWLNLTQNAFACVCEHQSFLQWVKDQRQLLVGAEQMMCAEPLDMEDMPVLSFRNATCQLSKTIISVSVVTVLLVSVVGVLVYKFYFHLMLLAGCKKYGRGESnullYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIQEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKLEKSLLRQQVELYRLLSRNTYLEWEDSVLGRHVFWRRLRKALLAGKPQSPEGTADAETNPQEATTST

Target ORF information:

RefSeq Version	NM_174198.6
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 4 (TLR4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174198.6

ORF Insert Sequence:

ATGATGGCGC GTGCCCGCCT GGCTGCGGCT CTGATCCCAG CCACGGCCAT CCTCTCCTGC 
CTGAGAACCG AGAGTTGGGA CCCTTGCGTA CAGGTTGTTC CTAACATTAG TTACCAGTGC 
ATGGAGCTGA ATCTCTACAA AATCCCCGAC AACATCCCCA TATCAACCAA GATGCTGGAC 
CTGAGCTTTA ACTACCTGAG ACATTTAGGC AGCCATAACT TCTCCAGCTT CCCAGAACTG 
CAAGTGCTGG ATTTATCCAG ATGTGAAATT AAGATTATTG AAGACGACAC ATTTCAGGGC 
CTAAACCACC TCTCCACCTT GATACTGACG GGAAACCCTA TCCAGAGTTT AGCCTGGGGA 
GCCTTTTCTG GGCTATCAAG TTTACAGAAG CTGGTGGCCG TGGAGACAAA CCTAGTCTCT 
CTAAATGACT TCCCCATTGG ACATCTCAAA AACTTGAAAG AGCTTAATGT GGCTCACAAT 
TTTATCCATT CCTTCAAGTT ACCTGAATAT TTTTCTAACC TGCCCAACCT GGAGCACTTG 
GATCTTTCTA ACAACAAAAT CCAAAATATT TATTATGAAG ATGTGAAAGT TCTACATCAA 
ATGCCCCTAC TCAACCTCTC TTTAGATTTG TCCTTGAACC CTTTAGACTT TATTGAACCA 
GGTACCTTTA AAGAAATTAA GCTCAATGGA TTGACTCTGC GAAGTAATTT TAACAGTTCA 
CATGTCATGA AAACCTGTAT TCAAGGTCTG GCTGGTTTAA AAACCAACCG GCTGGTTTTG 
GGAGAATTTA AAAATGAAAG GAAGTTGCAA AGATTTGACA GATCTTTCCT GGAGGGACTG 
TGCAACCTGA CCATTGAACA ATTCCGGATA GCGTACTTGG ACAAATTCTC AGGGGACGAT 
ACAGACTTAT TTAATTGTTT GGCAAATGTT TCTGTGATTT CTCTGTTGAG TATATCTTTA 
GGAAGTCTAC AAGCCCTTCT TAAAGATTTT AGATGGCAAC ACTTAGAAAT TATTAACTGT 
GACTTTGACA AGTTTCCTGC ATTGAAGCTC AGTTCTCTCA AAAAGTTTGT TTTCACAGAC 
AACAAAGATA TAAGCACTTT TACTGAGTTT CAGCTACCAA GCCTTCAGTA TCTAGATCTC 
AAAAGAAATC ACTTGAGTTT CAAGGGTTGC TGTTCTCACA CTGATTTTGG GACAACCAAC 
CTGAAGCATT TAGATCTGAG CTTCAATGAT GTCATTACCT TAGGTTCAAA CTTCATGGGC 
TTAGAGCAAC TAGAACACCT GGATTTTCAG CATTCCACTC TGAAACAGAT CAATGCTTTT 
TCAGCATTCC TATCACTCAG AAACCTCCGC TACCTTGATA TTTCTTATAC CAACATCCGC 
ATTGTCTTCC ATGGCATCTT TACTGGCTTA GTCAGTCTGC AAACCTTGAA AATGGCAGGC 
AACTCTTTTC AGAACAACTT GCTCCCTGAC ATCTTCACAG AACTGACTAA CTTAACCGTC 
TTGGACCTCT CTAAGTGTCA ACTGGAACAG GTAGCCCAGA CAGCATTTCA CTCCCTCTCT 
AGCCTTCAGG TGCTGAATAT GAGTCACAAC AAACTCTTGT CACTGGATAC ATTTCTTTAT 
GAACCACTCC ACTCGCTCCG GATCCTAGAC TGCAGYTTCA ACCGTATCAT GGCCTCTAAG 
GAGCAAGAAC TACAGAATTT GCCAAGGAGC CTCACTTGGC TAAATCTTAC TCAGAATGCA 
TTTGCTTGTG TTTGTGAACA TCAGAGTTTC CTGCAGTGGG TCAAGGACCA GAGGCAGCTC 
TTGGTGGGAG CTGAGCAAAT GATGTGTGCA GAGCCTTTAG ATATGGAGGA CATGCCAGTG 
CTTAGTTTCA GGAACGCCAC TTGTCAGCTG AGCAAGACGA TCATCAGTGT GTCGGTGGTC 
ACTGTGCTCC TGGTGTCTGT GGTAGGGGTC CTAGTCTACA AGTTCTATTT CCACCTGATG 
CTTCTTGCTG GCTGCAAAAA GTATGGCAGG GGCGAGAGCA YCTATGATGC CTTTGTCATC 
TACTCGAGCC AGGATGAAGA CTGGGTGCGG AATGAACTGG TAAAGAACTT GGAGGAGGGC 
GTGCCCCCCT TTCAGCTCTG CCTTCACTAC AGGGACTTTA TTCCTGGGGT GGCCATCGCC 
GCCAATATCA TCCAGGAGGG TTTCCACAAA AGCCGTAAGG TTATTGTCGT GGTGTCCCAG 
CACTTCATCC AGAGCCGATG GTGTATCTTC GAGTATGAGA TTGCCCAGAC CTGGCAGTTT 
CTGAGCAGCC GTGCTGGCAT CATCTTCATC GTCCTGCAGA AGCTGGAGAA GTCTCTCCTG 
CGGCAGCAGG TGGAGCTCTA TCGCCTTCTG AGCAGGAACA CCTACCTGGA GTGGGAGGAC 
AGTGTCCTGG GGCGGCATGT CTTCTGGAGA AGACTCAGAA AAGCCTTGCT GGCTGGTAAA 
CCCCAGAGTC CAGAAGGAAC AGCAGATGCA GAAACCAACC CGCAAGAAGC GACAACCTCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR4 ( XM_024995446.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851214.1 Bos taurus toll like receptor 4 (TLR4), transcript variant X1, mRNA.

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CloneID	OBa236926
Clone ID Related Accession (Same CDS sequence)	XM_024995446.1
Accession Version	XM_024995446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	toll-like receptor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTTC CCAGATGTGA AATTAAGATT ATTGAAGACG ACACATTTCA GGGCCTAAAC 
CACCTCTCCA CCTTGATACT GACGGGAAAC CCTATCCAGA GTTTAGCCTG GGGAGCCTTT 
TCTGGGCTAT CAAGTTTACA GAAGCTGGTG GCCGTGGAGA CAAACCTAGT CTCTCTAAAT 
GACTTCCCCA TTGGACATCT CAAAAACTTG AAAGAGCTTA ATGTGGCTCA CAATTTTATC 
CATTCCTTCA AGTTACCTGA ATATTTTTCT AACCTGCCCA ACCTGGAGCA CTTGGATCTT 
TCTAACAACA AAATCCAAAA TATTTATTAT GAAGATGTGA AAGTTCTACA TCAAATGCCC 
CTACTCAACC TCTCTTTAGA TTTGTCCTTG AACCCTTTAG ACTTTATTGA ACCAGGTACC 
TTTAAAGAAA TTAAGCTCAA TGGATTGACT CTGCGAAGTA ATTTTAACAG TTCACATGTC 
ATGAAAACCT GTATTCAAGG TCTGGCTGGT TTAAAAACCA ACCGGCTGGT TTTGGGAGAA 
TTTAAAAATG AAAGGAAGTT GCAAAGATTT GACAGATCTT TCCTGGAGGG ACTGTGCAAC 
CTGACCATTG AACAATTCCG GATAGCGTAC TTGGACAAAT TCTCAGGGGA CGATACAGAC 
TTATTTAATT GTTTGGCAAA TGTTTCTGTG ATTTCTCTGT TGAGTATATC TTTAGGAAGT 
CTACAAGCCC TTCTTAAAGA TTTTAGATGG CAACACTTAG AAATTATTAA CTGTGACTTT 
GACAAGTTTC CTGCATTGAA GCTCAGTTCT CTCAAAAAGT TTGTTTTCAC AGACAACAAA 
GATATAAGCA CTTTTACTGA GTTTCAGCTA CCAAGCCTTC AGTATCTAGA TCTCAAAAGA 
AATCACTTGA GTTTCAAGGG TTGCTGTTCT CACACTGATT TTGGGACAAC CAACCTGAAG 
CATTTAGATC TGAGCTTCAA TGATGTCATT ACCTTAGGTT CAAACTTCAT GGGCTTAGAG 
CAACTAGAAC ACCTGGATTT TCAGCATTCC ACTCTGAAAC AGATCAATGC TTTTTCAGCA 
TTCCTATCAC TCAGAAACCT CCGCTACCTT GATATTTCTT ATACCAACAT CCGCATTGTC 
TTCCATGGCA TCTTTACTGG CTTAGTCAGT CTGCAAACCT TGAAAATGGC AGGCAACTCT 
TTTCAGAACA ACTTGCTCCC TGACATCTTC ACAGAACTGA CTAACTTAAC CGTCTTGGAC 
CTCTCTAAGT GTCAACTGGA ACAGGTAGCC CAGACAGCAT TTCACTCCCT CTCTAGCCTT 
CAGGTGCTGA ATATGAGTCA CAACAAACTC TTGTCACTGG ATACATTTCT TTATGAACCA 
CTCCACTCGC TCCGGATCCT AGACTGCAGT TTCAACCGTA TCATGGCCTC TAAGGAGCAA 
GAACTACAGA ATTTGCCAAG GAGCCTCACT TGGCTAAATC TTACTCAGAA TGCATTTGCT 
TGTGTTTGTG AACATCAGAG TTTCCTGCAG TGGGTCAAGG ACCAGAGGCA GCTCTTGGTG 
GGAGCTGAGC AAATGATGTG TGCAGAGCCT TTAGATATGG AGGACATGCC AGTGCTTAGT 
TTCAGGAACG CCACTTGTCA GCTGAGCAAG ACGATCATCA GTGTGTCGGT GGTCACTGTG 
CTCCTGGTGT CTGTGGTAGG GGTCCTAGTC TACAAGTTCT ATTTCCACCT GATGCTTCTT 
GCTGGCTGCA AAAAGTATGG CAGGGGCGAG AGCACCTATG ATGCCTTTGT CATCTACTCG 
AGCCAGGATG AAGACTGGGT GCGGAATGAA CTGGTAAAGA ACTTGGAGGA GGGCGTGCCC 
CCCTTTCAGC TCTGCCTTCA CTACAGGGAC TTTATTCCTG GGGTGGCCAT CGCCGCCAAT 
ATCATCCAGG AGGGTTTCCA CAAAAGCCGT AAGGTTATTG TCGTGGTGTC CCAGCACTTC 
ATCCAGAGCC GATGGTGTAT CTTCGAGTAT GAGATTGCCC AGACCTGGCA GTTTCTGAGC 
AGCCGTGCTG GCATCATCTT CATCGTCCTG CAGAAGCTGG AGAAGTCTCT CCTGCGGCAG 
CAGGTGGAGC TCTATCGCCT TCTGAGCAGG AACACCTACC TGGAGTGGGA GGACAGTGTC 
CTGGGGCGGC ATGTCTTCTG GAGAAGACTC AGAAAAGCCT TGCTGGCTGG TAAACCCCAG 
AGTCCAGAAG GAACAGCAGA TGCAGAAACC AACCCGCAAG AAGCGACAAC CTCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851214.1
CDS	347..2626
Translation	MGFPRCEIKIIEDDTFQGLNHLSTLILTGNPIQSLAWGAFSGLSSLQKLVAVETNLVSLNDFPIGHLKNLKELNVAHNFIHSFKLPEYFSNLPNLEHLDLSNNKIQNIYYEDVKVLHQMPLLNLSLDLSLNPLDFIEPGTFKEIKLNGLTLRSNFNSSHVMKTCIQGLAGLKTNRLVLGEFKNERKLQRFDRSFLEGLCNLTIEQFRIAYLDKFSGDDTDLFNCLANVSVISLLSISLGSLQALLKDFRWQHLEIINCDFDKFPALKLSSLKKFVFTDNKDISTFTEFQLPSLQYLDLKRNHLSFKGCCSHTDFGTTNLKHLDLSFNDVITLGSNFMGLEQLEHLDFQHSTLKQINAFSAFLSLRNLRYLDISYTNIRIVFHGIFTGLVSLQTLKMAGNSFQNNLLPDIFTELTNLTVLDLSKCQLEQVAQTAFHSLSSLQVLNMSHNKLLSLDTFLYEPLHSLRILDCSFNRIMASKEQELQNLPRSLTWLNLTQNAFACVCEHQSFLQWVKDQRQLLVGAEQMMCAEPLDMEDMPVLSFRNATCQLSKTIISVSVVTVLLVSVVGVLVYKFYFHLMLLAGCKKYGRGESTYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIQEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKLEKSLLRQQVELYRLLSRNTYLEWEDSVLGRHVFWRRLRKALLAGKPQSPEGTADAETNPQEATTST

Target ORF information:

RefSeq Version	XM_024995446.1
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 4 (TLR4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995446.1

ORF Insert Sequence:

ATGGGATTTC CCAGATGTGA AATTAAGATT ATTGAAGACG ACACATTTCA GGGCCTAAAC 
CACCTCTCCA CCTTGATACT GACGGGAAAC CCTATCCAGA GTTTAGCCTG GGGAGCCTTT 
TCTGGGCTAT CAAGTTTACA GAAGCTGGTG GCCGTGGAGA CAAACCTAGT CTCTCTAAAT 
GACTTCCCCA TTGGACATCT CAAAAACTTG AAAGAGCTTA ATGTGGCTCA CAATTTTATC 
CATTCCTTCA AGTTACCTGA ATATTTTTCT AACCTGCCCA ACCTGGAGCA CTTGGATCTT 
TCTAACAACA AAATCCAAAA TATTTATTAT GAAGATGTGA AAGTTCTACA TCAAATGCCC 
CTACTCAACC TCTCTTTAGA TTTGTCCTTG AACCCTTTAG ACTTTATTGA ACCAGGTACC 
TTTAAAGAAA TTAAGCTCAA TGGATTGACT CTGCGAAGTA ATTTTAACAG TTCACATGTC 
ATGAAAACCT GTATTCAAGG TCTGGCTGGT TTAAAAACCA ACCGGCTGGT TTTGGGAGAA 
TTTAAAAATG AAAGGAAGTT GCAAAGATTT GACAGATCTT TCCTGGAGGG ACTGTGCAAC 
CTGACCATTG AACAATTCCG GATAGCGTAC TTGGACAAAT TCTCAGGGGA CGATACAGAC 
TTATTTAATT GTTTGGCAAA TGTTTCTGTG ATTTCTCTGT TGAGTATATC TTTAGGAAGT 
CTACAAGCCC TTCTTAAAGA TTTTAGATGG CAACACTTAG AAATTATTAA CTGTGACTTT 
GACAAGTTTC CTGCATTGAA GCTCAGTTCT CTCAAAAAGT TTGTTTTCAC AGACAACAAA 
GATATAAGCA CTTTTACTGA GTTTCAGCTA CCAAGCCTTC AGTATCTAGA TCTCAAAAGA 
AATCACTTGA GTTTCAAGGG TTGCTGTTCT CACACTGATT TTGGGACAAC CAACCTGAAG 
CATTTAGATC TGAGCTTCAA TGATGTCATT ACCTTAGGTT CAAACTTCAT GGGCTTAGAG 
CAACTAGAAC ACCTGGATTT TCAGCATTCC ACTCTGAAAC AGATCAATGC TTTTTCAGCA 
TTCCTATCAC TCAGAAACCT CCGCTACCTT GATATTTCTT ATACCAACAT CCGCATTGTC 
TTCCATGGCA TCTTTACTGG CTTAGTCAGT CTGCAAACCT TGAAAATGGC AGGCAACTCT 
TTTCAGAACA ACTTGCTCCC TGACATCTTC ACAGAACTGA CTAACTTAAC CGTCTTGGAC 
CTCTCTAAGT GTCAACTGGA ACAGGTAGCC CAGACAGCAT TTCACTCCCT CTCTAGCCTT 
CAGGTGCTGA ATATGAGTCA CAACAAACTC TTGTCACTGG ATACATTTCT TTATGAACCA 
CTCCACTCGC TCCGGATCCT AGACTGCAGT TTCAACCGTA TCATGGCCTC TAAGGAGCAA 
GAACTACAGA ATTTGCCAAG GAGCCTCACT TGGCTAAATC TTACTCAGAA TGCATTTGCT 
TGTGTTTGTG AACATCAGAG TTTCCTGCAG TGGGTCAAGG ACCAGAGGCA GCTCTTGGTG 
GGAGCTGAGC AAATGATGTG TGCAGAGCCT TTAGATATGG AGGACATGCC AGTGCTTAGT 
TTCAGGAACG CCACTTGTCA GCTGAGCAAG ACGATCATCA GTGTGTCGGT GGTCACTGTG 
CTCCTGGTGT CTGTGGTAGG GGTCCTAGTC TACAAGTTCT ATTTCCACCT GATGCTTCTT 
GCTGGCTGCA AAAAGTATGG CAGGGGCGAG AGCACCTATG ATGCCTTTGT CATCTACTCG 
AGCCAGGATG AAGACTGGGT GCGGAATGAA CTGGTAAAGA ACTTGGAGGA GGGCGTGCCC 
CCCTTTCAGC TCTGCCTTCA CTACAGGGAC TTTATTCCTG GGGTGGCCAT CGCCGCCAAT 
ATCATCCAGG AGGGTTTCCA CAAAAGCCGT AAGGTTATTG TCGTGGTGTC CCAGCACTTC 
ATCCAGAGCC GATGGTGTAT CTTCGAGTAT GAGATTGCCC AGACCTGGCA GTTTCTGAGC 
AGCCGTGCTG GCATCATCTT CATCGTCCTG CAGAAGCTGG AGAAGTCTCT CCTGCGGCAG 
CAGGTGGAGC TCTATCGCCT TCTGAGCAGG AACACCTACC TGGAGTGGGA GGACAGTGTC 
CTGGGGCGGC ATGTCTTCTG GAGAAGACTC AGAAAAGCCT TGCTGGCTGG TAAACCCCAG 
AGTCCAGAAG GAACAGCAGA TGCAGAAACC AACCCGCAAG AAGCGACAAC CTCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR4 ( XM_005210586.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210643.1 Bos taurus toll like receptor 4 (TLR4), transcript variant X2, mRNA.

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CloneID	OBa236927
Clone ID Related Accession (Same CDS sequence)	XM_005210586.4
Accession Version	XM_005210586.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	toll-like receptor 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210586.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTAC TCAACCTCTC TTTAGATTTG TCCTTGAACC CTTTAGACTT TATTGAACCA 
GGTACCTTTA AAGAAATTAA GCTCAATGGA TTGACTCTGC GAAGTAATTT TAACAGTTCA 
CATGTCATGA AAACCTGTAT TCAAGGTCTG GCTGGTTTAA AAACCAACCG GCTGGTTTTG 
GGAGAATTTA AAAATGAAAG GAAGTTGCAA AGATTTGACA GATCTTTCCT GGAGGGACTG 
TGCAACCTGA CCATTGAACA ATTCCGGATA GCGTACTTGG ACAAATTCTC AGGGGACGAT 
ACAGACTTAT TTAATTGTTT GGCAAATGTT TCTGTGATTT CTCTGTTGAG TATATCTTTA 
GGAAGTCTAC AAGCCCTTCT TAAAGATTTT AGATGGCAAC ACTTAGAAAT TATTAACTGT 
GACTTTGACA AGTTTCCTGC ATTGAAGCTC AGTTCTCTCA AAAAGTTTGT TTTCACAGAC 
AACAAAGATA TAAGCACTTT TACTGAGTTT CAGCTACCAA GCCTTCAGTA TCTAGATCTC 
AAAAGAAATC ACTTGAGTTT CAAGGGTTGC TGTTCTCACA CTGATTTTGG GACAACCAAC 
CTGAAGCATT TAGATCTGAG CTTCAATGAT GTCATTACCT TAGGTTCAAA CTTCATGGGC 
TTAGAGCAAC TAGAACACCT GGATTTTCAG CATTCCACTC TGAAACAGAT CAATGCTTTT 
TCAGCATTCC TATCACTCAG AAACCTCCGC TACCTTGATA TTTCTTATAC CAACATCCGC 
ATTGTCTTCC ATGGCATCTT TACTGGCTTA GTCAGTCTGC AAACCTTGAA AATGGCAGGC 
AACTCTTTTC AGAACAACTT GCTCCCTGAC ATCTTCACAG AACTGACTAA CTTAACCGTC 
TTGGACCTCT CTAAGTGTCA ACTGGAACAG GTAGCCCAGA CAGCATTTCA CTCCCTCTCT 
AGCCTTCAGG TGCTGAATAT GAGTCACAAC AAACTCTTGT CACTGGATAC ATTTCTTTAT 
GAACCACTCC ACTCGCTCCG GATCCTAGAC TGCAGTTTCA ACCGTATCAT GGCCTCTAAG 
GAGCAAGAAC TACAGAATTT GCCAAGGAGC CTCACTTGGC TAAATCTTAC TCAGAATGCA 
TTTGCTTGTG TTTGTGAACA TCAGAGTTTC CTGCAGTGGG TCAAGGACCA GAGGCAGCTC 
TTGGTGGGAG CTGAGCAAAT GATGTGTGCA GAGCCTTTAG ATATGGAGGA CATGCCAGTG 
CTTAGTTTCA GGAACGCCAC TTGTCAGCTG AGCAAGACGA TCATCAGTGT GTCGGTGGTC 
ACTGTGCTCC TGGTGTCTGT GGTAGGGGTC CTAGTCTACA AGTTCTATTT CCACCTGATG 
CTTCTTGCTG GCTGCAAAAA GTATGGCAGG GGCGAGAGCA CCTATGATGC CTTTGTCATC 
TACTCGAGCC AGGATGAAGA CTGGGTGCGG AATGAACTGG TAAAGAACTT GGAGGAGGGC 
GTGCCCCCCT TTCAGCTCTG CCTTCACTAC AGGGACTTTA TTCCTGGGGT GGCCATCGCC 
GCCAATATCA TCCAGGAGGG TTTCCACAAA AGCCGTAAGG TTATTGTCGT GGTGTCCCAG 
CACTTCATCC AGAGCCGATG GTGTATCTTC GAGTATGAGA TTGCCCAGAC CTGGCAGTTT 
CTGAGCAGCC GTGCTGGCAT CATCTTCATC GTCCTGCAGA AGCTGGAGAA GTCTCTCCTG 
CGGCAGCAGG TGGAGCTCTA TCGCCTTCTG AGCAGGAACA CCTACCTGGA GTGGGAGGAC 
AGTGTCCTGG GGCGGCATGT CTTCTGGAGA AGACTCAGAA AAGCCTTGCT GGCTGGTAAA 
CCCCAGAGTC CAGAAGGAAC AGCAGATGCA GAAACCAACC CGCAAGAAGC GACAACCTCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210643.1
CDS	721..2646
Translation	MPLLNLSLDLSLNPLDFIEPGTFKEIKLNGLTLRSNFNSSHVMKTCIQGLAGLKTNRLVLGEFKNERKLQRFDRSFLEGLCNLTIEQFRIAYLDKFSGDDTDLFNCLANVSVISLLSISLGSLQALLKDFRWQHLEIINCDFDKFPALKLSSLKKFVFTDNKDISTFTEFQLPSLQYLDLKRNHLSFKGCCSHTDFGTTNLKHLDLSFNDVITLGSNFMGLEQLEHLDFQHSTLKQINAFSAFLSLRNLRYLDISYTNIRIVFHGIFTGLVSLQTLKMAGNSFQNNLLPDIFTELTNLTVLDLSKCQLEQVAQTAFHSLSSLQVLNMSHNKLLSLDTFLYEPLHSLRILDCSFNRIMASKEQELQNLPRSLTWLNLTQNAFACVCEHQSFLQWVKDQRQLLVGAEQMMCAEPLDMEDMPVLSFRNATCQLSKTIISVSVVTVLLVSVVGVLVYKFYFHLMLLAGCKKYGRGESTYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIQEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKLEKSLLRQQVELYRLLSRNTYLEWEDSVLGRHVFWRRLRKALLAGKPQSPEGTADAETNPQEATTST

Target ORF information:

RefSeq Version	XM_005210586.4
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 4 (TLR4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210586.4

ORF Insert Sequence:

ATGCCCCTAC TCAACCTCTC TTTAGATTTG TCCTTGAACC CTTTAGACTT TATTGAACCA 
GGTACCTTTA AAGAAATTAA GCTCAATGGA TTGACTCTGC GAAGTAATTT TAACAGTTCA 
CATGTCATGA AAACCTGTAT TCAAGGTCTG GCTGGTTTAA AAACCAACCG GCTGGTTTTG 
GGAGAATTTA AAAATGAAAG GAAGTTGCAA AGATTTGACA GATCTTTCCT GGAGGGACTG 
TGCAACCTGA CCATTGAACA ATTCCGGATA GCGTACTTGG ACAAATTCTC AGGGGACGAT 
ACAGACTTAT TTAATTGTTT GGCAAATGTT TCTGTGATTT CTCTGTTGAG TATATCTTTA 
GGAAGTCTAC AAGCCCTTCT TAAAGATTTT AGATGGCAAC ACTTAGAAAT TATTAACTGT 
GACTTTGACA AGTTTCCTGC ATTGAAGCTC AGTTCTCTCA AAAAGTTTGT TTTCACAGAC 
AACAAAGATA TAAGCACTTT TACTGAGTTT CAGCTACCAA GCCTTCAGTA TCTAGATCTC 
AAAAGAAATC ACTTGAGTTT CAAGGGTTGC TGTTCTCACA CTGATTTTGG GACAACCAAC 
CTGAAGCATT TAGATCTGAG CTTCAATGAT GTCATTACCT TAGGTTCAAA CTTCATGGGC 
TTAGAGCAAC TAGAACACCT GGATTTTCAG CATTCCACTC TGAAACAGAT CAATGCTTTT 
TCAGCATTCC TATCACTCAG AAACCTCCGC TACCTTGATA TTTCTTATAC CAACATCCGC 
ATTGTCTTCC ATGGCATCTT TACTGGCTTA GTCAGTCTGC AAACCTTGAA AATGGCAGGC 
AACTCTTTTC AGAACAACTT GCTCCCTGAC ATCTTCACAG AACTGACTAA CTTAACCGTC 
TTGGACCTCT CTAAGTGTCA ACTGGAACAG GTAGCCCAGA CAGCATTTCA CTCCCTCTCT 
AGCCTTCAGG TGCTGAATAT GAGTCACAAC AAACTCTTGT CACTGGATAC ATTTCTTTAT 
GAACCACTCC ACTCGCTCCG GATCCTAGAC TGCAGTTTCA ACCGTATCAT GGCCTCTAAG 
GAGCAAGAAC TACAGAATTT GCCAAGGAGC CTCACTTGGC TAAATCTTAC TCAGAATGCA 
TTTGCTTGTG TTTGTGAACA TCAGAGTTTC CTGCAGTGGG TCAAGGACCA GAGGCAGCTC 
TTGGTGGGAG CTGAGCAAAT GATGTGTGCA GAGCCTTTAG ATATGGAGGA CATGCCAGTG 
CTTAGTTTCA GGAACGCCAC TTGTCAGCTG AGCAAGACGA TCATCAGTGT GTCGGTGGTC 
ACTGTGCTCC TGGTGTCTGT GGTAGGGGTC CTAGTCTACA AGTTCTATTT CCACCTGATG 
CTTCTTGCTG GCTGCAAAAA GTATGGCAGG GGCGAGAGCA CCTATGATGC CTTTGTCATC 
TACTCGAGCC AGGATGAAGA CTGGGTGCGG AATGAACTGG TAAAGAACTT GGAGGAGGGC 
GTGCCCCCCT TTCAGCTCTG CCTTCACTAC AGGGACTTTA TTCCTGGGGT GGCCATCGCC 
GCCAATATCA TCCAGGAGGG TTTCCACAAA AGCCGTAAGG TTATTGTCGT GGTGTCCCAG 
CACTTCATCC AGAGCCGATG GTGTATCTTC GAGTATGAGA TTGCCCAGAC CTGGCAGTTT 
CTGAGCAGCC GTGCTGGCAT CATCTTCATC GTCCTGCAGA AGCTGGAGAA GTCTCTCCTG 
CGGCAGCAGG TGGAGCTCTA TCGCCTTCTG AGCAGGAACA CCTACCTGGA GTGGGAGGAC 
AGTGTCCTGG GGCGGCATGT CTTCTGGAGA AGACTCAGAA AAGCCTTGCT GGCTGGTAAA 
CCCCAGAGTC CAGAAGGAAC AGCAGATGCA GAAACCAACC CGCAAGAAGC GACAACCTCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dominance effects estimation of TLR4 and CACNA2D1 genes for health and production traits using logistic regression.
Journal of genetics96(6)1027-1031(2017 Dec)
Bagheri M,Zahmatkesh A

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TLR4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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