Tlr4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tlr4
Entrez Gene ID	29260
Full Name	toll-like receptor 4
General protein information	Preferred Names toll-like receptor 4 Names toll-like receptor 4 toll4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q24
Summary	receptor that functions as the major upstream sensor for hemorrhagic shock and lipopolysaccharide [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019178.1	NP_062051.1	toll-like receptor 4 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tlr4	NP_067272.1
Danio rerio (zebrafish)	tlr4ba	NP_001124523.1
Pan troglodytes (chimpanzee)	TLR4	NP_001138335.1
Gallus gallus (chicken)	TLR4	NP_001025864.1
Homo sapiens (human)	TLR4	NP_612564.1
	TLR4	NP_001032169.1
Rattus norvegicus (Norway rat)	Tlr4	NP_062051.1
Danio rerio (zebrafish)	tlr4al	XP_001919699.4
Danio rerio (zebrafish)	tlr4bb	NP_997978.1
Canis lupus familiaris (dog)	TLR4	NP_001002950.2
Bos taurus (cattle)	TLR4	NP_776623.5

Gene Ontology
Bibliography

Related articles in PubMed

Correlation of TLR4 and KLF7 in Inflammation Induced by Obesity.
Wang C, Ha X, Li W, Xu P, Gu Y, Wang T, Wang Y, Xie J, Zhang J
Inflammation40(1)42-51(2017 Feb)

SIRT1 regulates lipopolysaccharide-induced CD40 expression in renal medullary collecting duct cells by suppressing the TLR4-NF-?B signaling pathway.
Lin QQ, Geng YW, Jiang ZW, Tian ZJ
Life sciences170100-107(2017 Feb)

Prx1 promotes the proliferation and migration of vascular smooth muscle cells in a TLR4-dependent manner.
Zhu Z, Zheng X, Li D, Wang T, Xu R, Piao H, Liu K
Molecular medicine reports15(1)345-351(2017 Jan)

AMPK activation by metformin inhibits local innate immune responses in the isolated rat heart by suppression of TLR 4-related pathway.
Vaez H, Najafi M, Rameshrad M, Toutounchi NS, Garjani M, Barar J, Garjani A
International immunopharmacology40501-507(2016 Nov)

Effects of ?-3 fatty acids on toll-like receptor 4 and nuclear factor-?B p56 in lungs of rats with severe acute pancreatitis.
Wang B, Wu XW, Guo MX, Li ML, Xu XB, Jin XX, Zhang XH
World journal of gastroenterology22(44)9784-9793(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

miR-146a improves intestine epithelial cells survival under ischemia and I/R injury through inhibition TLR4, TRAF6, and p-IkappaBalpha, subsequently leading to decreased NF-kappaB p65 nuclear translocation.
Title: MiR-146a protects small intestine against ischemia/reperfusion injury by down-regulating TLR4/TRAF6/NF-?B pathway.

Intraventricular hemorrhage-induced hypersecretion of CSF is mediated by TLR4-dependent activation of SPAK, which binds, phosphorylates, and stimulates the NKCC1 co-transporter at the CPE apical membrane.
Title: Inflammation-dependent cerebrospinal fluid hypersecretion by the choroid plexus epithelium in posthemorrhagic hydrocephalus.

Although TLR4 was not a critical determinant of excessive drinking, it was important in the acute sedative effects of
Title: Genetic and Pharmacologic Manipulation of TLR4 Has Minimal Impact on Ethanol Consumption in Rodents.

The opening of ATP-sensitive K+ channels protects cardiomyocytes against the high glucose-induced injury and inflammation by inhibiting the ROS-TLR4-necroptosis pathway.
Title: The Opening of ATP-Sensitive K+ Channels Protects H9c2 Cardiac Cells Against the High Glucose-Induced Injury and Inflammation by Inhibiting the ROS-TLR4-Necroptosis Pathway.

these findings indicate that modifying TLR4 gene expression in the periaqueductal gray stimulates expression of the downstream signaling molecule, GAD67, which decreases Glu levels and increases GABA levels. This mechanism may explain the inhibition of withdrawal syndrome in morphine-dependent rats.
Title: Injection of Toll-like receptor 4 siRNA into the ventrolateral periaqueductal gray attenuates withdrawal syndrome in morphine-dependent rats.

The following Tlr4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tlr4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13506	NM_019178.1 Latest version!	Rattus norvegicus toll-like receptor 4 (Tlr4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$489.30 $699.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tlr4 ( NM_019178.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062051.1 Rattus norvegicus toll-like receptor 4 (Tlr4), mRNA.

Price & Availability↑

CloneID	ORa13506
Clone ID Related Accession (Same CDS sequence)	NM_019178.1
Accession Version	NM_019178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-05
Organism	Rattus norvegicus(Norway rat)
Product	toll-like receptor 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF057025.1. Sequence Note: This sequence has been modified as follows: removed 22 bp suspected to be vector contamination from the 5' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GQ175880.1, AF057025.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGATGCCTC TCTTGCATCT GGCTGGGACT CTGATCATGG CATTGTTCCT TTCCTGCCTG 
AGACCAGGAA GCTTGAATCC CTGCATAGAG GTACTTCCTA ATATTACCTA CCAATGCATG 
GATCAGAATC TCAGCAAAAT CCCTCATGAC ATCCCTTATT CAACCAAGAA CCTAGATCTG 
AGCTTCAACC CCCTGAAGAT CTTAAGAAGC TATAGCTTCA CCAATTTCTC ACAACTTCAG 
TGGCTGGATT TATCCAGGTG TGAAATTGAG ACAATTGAAG ACAAGGCATG GCATGGCTTA 
AACCAGCTCT CAACCTTGGT ACTGACAGGA AACCCTATCA AGAGTTTTTC CCCAGGAAGT 
TTTTCTGGAC TAACAAATTT AGAGAATCTG GTGGCTGTGG AGACAAAAAT GACCTCTCTA 
GAGGGTTTCC ATATTGGACA GCTTATATCC TTAAAGAAAC TAAATGTGGC TCATAATCTT 
ATACATTCCT TTAAGTTGCC TGAATATTTT TCTAATCTGA CAAACCTAGA ACATGTGGAT 
CTTTCTTATA ACTATATTCA AACTATTTCT GTCAAAGACT TACAGTTTCT ACGTGAAAAT 
CCCCAAGTCA ATCTCTCTTT AGACCTGTCT TTAAACCCAA TTGACTCCAT TCAAGCCCAA 
GCCTTTCAGG GAATTAGGCT CCATGAATTG ACTCTAAGAA GTAATTTTAA TAGCTCAAAT 
GTACTGAAAA TGTGCCTTCA AAACATGACT GGTTTACATG TCCATCGGTT GATCTTGGGA 
GAATTTAAAA ATGAAAGGAA TCTGGAAAGT TTTGACCGTT CTGTCATGGA AGGACTATGC 
AATGTGAGCA TTGATGAGTT CAGGTTAACA TATATAAATC ATTTTTCAGA TGATATTTAT 
AATCTCAATT GCTTGGCAAA TATTTCTGCA ATGTCTTTCA CAGGTGTACA TATAAAACAC 
ATAGCAGATG TTCCTAGGCA TTTCAAATGG CAATCCTTAT CAATCATTAG ATGTCATCTT 
AAGCCTTTTC CAAAGCTGAG TCTACCTTTT CTTAAAAGTT GGACTTTAAC TACCAACAGA 
GAGGATATCA GCTTTGGTCA GTTGGCTCTG CCAAGTCTCA GATATCTAGA TCTTAGTAGA 
AATGCCATGA GCTTTAGAGG TTGCTGTTCT TATTCTGATT TTGGAACAAA CAACCTGAAG 
TACTTAGACC TCAGCTTCAA TGGTGTCATC CTGATGAGTG CCAACTTCAT GGGTCTAGAA 
GAGCTGGAAT ACCTGGACTT TCAGCACTCC ACTTTAAAAA AGGTCACAGA ATTCTCAGTG 
TTCTTATCTC TTGAAAAACT TCTTTACCTT GACATCTCTT ACACTAATAC CAAAATTGAC 
TTTGATGGCA TATTTCTTGG CTTGATCAGT CTCAACACTT TAAAAATGGC TGGCAATTCT 
TTCAAAGACA ACACCCTTTC AAATGTCTTT ACAAACACAA CAAACTTAAC ATTCCTGGAT 
CTTTCTAAAT GCCAACTGGA ACAGATATCT AGGGGGGTAT TTGACACACT CTACAGACTC 
CAGTTATTAA ACATGAGTCA CAACAACCTA CTGTTTCTGG ATCCATCCCA TTATAAACAG 
CTGTACTCCC TCAGGACTCT TGATTGCAGT TTCAATCGCA TAGAGACATC CAAAGGAATA 
CTGCAACATT TTCCAAAGAG TCTAGCCGTC TTCAATCTGA CTAATAATTC TGTTGCTTGT 
ATATGTGAAT ATCAGAATTT CTTGCAGTGG GTCAAGGACC AGAAAATGTT CTTGGTGAAT 
GTTGAACAAA TGAAATGTGC ATCACCTATA GACATGAAGG CCTCCCTGGT GTTGGATTTT 
ACGAATTCCA CCTGTTATAT ATACAAGACT ATCATCAGTG TATCGGTGGT CAGTGTGCTT 
GTGGTAGCCA CTGTAGCATT TCTGATATAC CACTTCTATT TTCACCTGAT ACTTATTGCT 
GGCTGTAAAA AGTACAGCAG AGGAGAAAGC ATCTATGATG CATTTGTGAT CTACTCGAGC 
CAGAATGAGG ACTGGGTGAG AAACGAGCTG GTAAAGAATT TAGAAGAAGG AGTGCCCCGC 
TTTCAGCTTT GCCTTCATTA CAGGGACTTT ATTCCTGGTG TAGCCATTGC TGCCAACATC 
ATCCAGGAAG GCTTCCACAA GAGCCGGAAA GTTATTGTGG TGGTGTCTAG ACACTTTATC 
CAGAGCCGTT GGTGTATCTT TGAATATGAG ATTGCTCAGA CATGGCAGTT TCTGAGTAGC 
CGCTCTGGCA TCATCTTCAT TGTCCTTGAG AAAGTGGAGA AGTCCTTGCT GAGGCAGCAG 
GTCGAATTGT ATCGCCTTCT TAGCAGAAAC ACCTACCTCG AGTGGGAGGA CAATGCTCTG 
GGGAGGCACA TCTTCTGGAG AAGACTCAAA AAAGCCCTGT TGGATGGAAA AGCCTTGAAT 
CCAGATGAAA CATCAGAGGA AGAACAAGAA GCAACAACTT TGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062051.1
CDS	238..2745
Translation	MMPLLHLAGTLIMALFLSCLRPGSLNPCIEVLPNITYQCMDQNLSKIPHDIPYSTKNLDLSFNPLKILRSYSFTNFSQLQWLDLSRCEIETIEDKAWHGLNQLSTLVLTGNPIKSFSPGSFSGLTNLENLVAVETKMTSLEGFHIGQLISLKKLNVAHNLIHSFKLPEYFSNLTNLEHVDLSYNYIQTISVKDLQFLRENPQVNLSLDLSLNPIDSIQAQAFQGIRLHELTLRSNFNSSNVLKMCLQNMTGLHVHRLILGEFKNERNLESFDRSVMEGLCNVSIDEFRLTYINHFSDDIYNLNCLANISAMSFTGVHIKHIADVPRHFKWQSLSIIRCHLKPFPKLSLPFLKSWTLTTNREDISFGQLALPSLRYLDLSRNAMSFRGCCSYSDFGTNNLKYLDLSFNGVILMSANFMGLEELEYLDFQHSTLKKVTEFSVFLSLEKLLYLDISYTNTKIDFDGIFLGLISLNTLKMAGNSFKDNTLSNVFTNTTNLTFLDLSKCQLEQISRGVFDTLYRLQLLNMSHNNLLFLDPSHYKQLYSLRTLDCSFNRIETSKGILQHFPKSLAVFNLTNNSVACICEYQNFLQWVKDQKMFLVNVEQMKCASPIDMKASLVLDFTNSTCYIYKTIISVSVVSVLVVATVAFLIYHFYFHLILIAGCKKYSRGESIYDAFVIYSSQNEDWVRNELVKNLEEGVPRFQLCLHYRDFIPGVAIAANIIQEGFHKSRKVIVVVSRHFIQSRWCIFEYEIAQTWQFLSSRSGIIFIVLEKVEKSLLRQQVELYRLLSRNTYLEWEDNALGRHIFWRRLKKALLDGKALNPDETSEEEQEATTLT

Target ORF information:

RefSeq Version	NM_019178.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus toll-like receptor 4 (Tlr4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019178.1

ORF Insert Sequence:

ATGATGCCTC TCTTGCATCT GGCTGGGACT CTGATCATGG CATTGTTCCT TTCCTGCCTG 
AGACCAGGAA GCTTGAATCC CTGCATAGAG GTACTTCCTA ATATTACCTA CCAATGCATG 
GATCAGAATC TCAGCAAAAT CCCTCATGAC ATCCCTTATT CAACCAAGAA CCTAGATCTG 
AGCTTCAACC CCCTGAAGAT CTTAAGAAGC TATAGCTTCA CCAATTTCTC ACAACTTCAG 
TGGCTGGATT TATCCAGGTG TGAAATTGAG ACAATTGAAG ACAAGGCATG GCATGGCTTA 
AACCAGCTCT CAACCTTGGT ACTGACAGGA AACCCTATCA AGAGTTTTTC CCCAGGAAGT 
TTTTCTGGAC TAACAAATTT AGAGAATCTG GTGGCTGTGG AGACAAAAAT GACCTCTCTA 
GAGGGTTTCC ATATTGGACA GCTTATATCC TTAAAGAAAC TAAATGTGGC TCATAATCTT 
ATACATTCCT TTAAGTTGCC TGAATATTTT TCTAATCTGA CAAACCTAGA ACATGTGGAT 
CTTTCTTATA ACTATATTCA AACTATTTCT GTCAAAGACT TACAGTTTCT ACGTGAAAAT 
CCCCAAGTCA ATCTCTCTTT AGACCTGTCT TTAAACCCAA TTGACTCCAT TCAAGCCCAA 
GCCTTTCAGG GAATTAGGCT CCATGAATTG ACTCTAAGAA GTAATTTTAA TAGCTCAAAT 
GTACTGAAAA TGTGCCTTCA AAACATGACT GGTTTACATG TCCATCGGTT GATCTTGGGA 
GAATTTAAAA ATGAAAGGAA TCTGGAAAGT TTTGACCGTT CTGTCATGGA AGGACTATGC 
AATGTGAGCA TTGATGAGTT CAGGTTAACA TATATAAATC ATTTTTCAGA TGATATTTAT 
AATCTCAATT GCTTGGCAAA TATTTCTGCA ATGTCTTTCA CAGGTGTACA TATAAAACAC 
ATAGCAGATG TTCCTAGGCA TTTCAAATGG CAATCCTTAT CAATCATTAG ATGTCATCTT 
AAGCCTTTTC CAAAGCTGAG TCTACCTTTT CTTAAAAGTT GGACTTTAAC TACCAACAGA 
GAGGATATCA GCTTTGGTCA GTTGGCTCTG CCAAGTCTCA GATATCTAGA TCTTAGTAGA 
AATGCCATGA GCTTTAGAGG TTGCTGTTCT TATTCTGATT TTGGAACAAA CAACCTGAAG 
TACTTAGACC TCAGCTTCAA TGGTGTCATC CTGATGAGTG CCAACTTCAT GGGTCTAGAA 
GAGCTGGAAT ACCTGGACTT TCAGCACTCC ACTTTAAAAA AGGTCACAGA ATTCTCAGTG 
TTCTTATCTC TTGAAAAACT TCTTTACCTT GACATCTCTT ACACTAATAC CAAAATTGAC 
TTTGATGGCA TATTTCTTGG CTTGATCAGT CTCAACACTT TAAAAATGGC TGGCAATTCT 
TTCAAAGACA ACACCCTTTC AAATGTCTTT ACAAACACAA CAAACTTAAC ATTCCTGGAT 
CTTTCTAAAT GCCAACTGGA ACAGATATCT AGGGGGGTAT TTGACACACT CTACAGACTC 
CAGTTATTAA ACATGAGTCA CAACAACCTA CTGTTTCTGG ATCCATCCCA TTATAAACAG 
CTGTACTCCC TCAGGACTCT TGATTGCAGT TTCAATCGCA TAGAGACATC CAAAGGAATA 
CTGCAACATT TTCCAAAGAG TCTAGCCGTC TTCAATCTGA CTAATAATTC TGTTGCTTGT 
ATATGTGAAT ATCAGAATTT CTTGCAGTGG GTCAAGGACC AGAAAATGTT CTTGGTGAAT 
GTTGAACAAA TGAAATGTGC ATCACCTATA GACATGAAGG CCTCCCTGGT GTTGGATTTT 
ACGAATTCCA CCTGTTATAT ATACAAGACT ATCATCAGTG TATCGGTGGT CAGTGTGCTT 
GTGGTAGCCA CTGTAGCATT TCTGATATAC CACTTCTATT TTCACCTGAT ACTTATTGCT 
GGCTGTAAAA AGTACAGCAG AGGAGAAAGC ATCTATGATG CATTTGTGAT CTACTCGAGC 
CAGAATGAGG ACTGGGTGAG AAACGAGCTG GTAAAGAATT TAGAAGAAGG AGTGCCCCGC 
TTTCAGCTTT GCCTTCATTA CAGGGACTTT ATTCCTGGTG TAGCCATTGC TGCCAACATC 
ATCCAGGAAG GCTTCCACAA GAGCCGGAAA GTTATTGTGG TGGTGTCTAG ACACTTTATC 
CAGAGCCGTT GGTGTATCTT TGAATATGAG ATTGCTCAGA CATGGCAGTT TCTGAGTAGC 
CGCTCTGGCA TCATCTTCAT TGTCCTTGAG AAAGTGGAGA AGTCCTTGCT GAGGCAGCAG 
GTCGAATTGT ATCGCCTTCT TAGCAGAAAC ACCTACCTCG AGTGGGAGGA CAATGCTCTG 
GGGAGGCACA TCTTCTGGAG AAGACTCAAA AAAGCCCTGT TGGATGGAAA AGCCTTGAAT 
CCAGATGAAA CATCAGAGGA AGAACAAGAA GCAACAACTT TGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Correlation of TLR4 and KLF7 in Inflammation Induced by Obesity.
Inflammation40(1)42-51(2017 Feb)
Wang C,Ha X,Li W,Xu P,Gu Y,Wang T,Wang Y,Xie J,Zhang J

SIRT1 regulates lipopolysaccharide-induced CD40 expression in renal medullary collecting duct cells by suppressing the TLR4-NF-?B signaling pathway.
Life sciences170100-107(2017 Feb)
Lin QQ,Geng YW,Jiang ZW,Tian ZJ

Prx1 promotes the proliferation and migration of vascular smooth muscle cells in a TLR4-dependent manner.
Molecular medicine reports15(1)345-351(2017 Jan)
Zhu Z,Zheng X,Li D,Wang T,Xu R,Piao H,Liu K

AMPK activation by metformin inhibits local innate immune responses in the isolated rat heart by suppression of TLR 4-related pathway.
International immunopharmacology40501-507(2016 Nov)
Vaez H,Najafi M,Rameshrad M,Toutounchi NS,Garjani M,Barar J,Garjani A

Effects of ?-3 fatty acids on toll-like receptor 4 and nuclear factor-?B p56 in lungs of rats with severe acute pancreatitis.
World journal of gastroenterology22(44)9784-9793(2016 Nov)
Wang B,Wu XW,Guo MX,Li ML,Xu XB,Jin XX,Zhang XH

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Tlr4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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