gRNAs
The following SLC5A3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC5A3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu16135 | |
| Clone ID Related Accession (Same CDS sequence) | NM_006933.7 , NM_006933.6 | |
| Accession Version | NM_006933.7 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-26 | |
| Organism | Homo sapiens(human) | |
| Product | sodium/myo-inositol cotransporter | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP000467.1, KF457230.1, KF457228.1, KF457229.1 and AP000315.1. On Nov 23, 2018 this sequence version replaced NM_006933.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA570729.1, SRR1660807.242830.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000381151.5/ ENSP00000370543.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length. | |
1 | ATGAGAGCTG TACTGGACAC AGCAGACATT GCCATAGTGG CCCTGTATTT TATCCTGGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_008864.4 |
| CDS | 505..2661 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_006933.7 |
1 | ATGAGAGCTG TACTGGACAC AGCAGACATT GCCATAGTGG CCCTGTATTT TATCCTGGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu16135 | |
| Clone ID Related Accession (Same CDS sequence) | NM_006933.7 , NM_006933.6 | |
| Accession Version | NM_006933.6 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-02 | |
| Organism | Homo sapiens(human) | |
| Product | sodium/myo-inositol cotransporter | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP000467.1, KF457230.1, KF457228.1, KF457229.1 and AP000315.1. On Apr 19, 2014 this sequence version replaced NM_006933.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.242830.1, SRR1660803.164476.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length. | |
1 | ATGAGAGCTG TACTGGACAC AGCAGACATT GCCATAGTGG CCCTGTATTT TATCCTGGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_008864.4 |
| CDS | 560..2716 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_006933.6 |
1 | ATGAGAGCTG TACTGGACAC AGCAGACATT GCCATAGTGG CCCTGTATTT TATCCTGGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
SMIT1 Modifies KCNQ Channel Function and Pharmacology by Physical Interaction with the Pore. |
Sodium-myoinositol cotransporter-1, SMIT1, mediates the production of reactive oxygen species induced by hyperglycemia in the heart. |
Osmoregulatory inositol transporter SMIT1 modulates electrical activity by adjusting PI(4,5)P2 levels. |
Hypotonic activation of the myo-inositol transporter SLC5A3 in HEK293 cells probed by cell volumetry, confocal and super-resolution microscopy. |
The transport mechanism of the human sodium/myo-inositol transporter 2 (SMIT2/SGLT6), a member of the LeuT structural family. |
