SIM2 cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SIM2
Entrez Gene ID	6493
Full Name	single-minded family bHLH transcription factor 2
Synonyms	HMC13F06,HMC29C01,SIM,bHLHe15
General protein information	Preferred Names single-minded family bHLH transcription factor 2 Names single-minded homolog 2 class E basic helix-loop-helix protein 15 transcription factor SIM2
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 21 Map Location: 21q22.13
Summary	This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014].
Disorder MIM:	600892

mRNA and Protein(s)

mRNA	Protein	Name
XM_017028442.1	XP_016883931.1	single-minded homolog 2 isoform X2
XM_017028442.2	XP_016883931.1	single-minded homolog 2 isoform X2
NM_009586.5	NP_033664.2	single-minded homolog 2 short isoform
NM_005069.5	NP_005060.1	single-minded homolog 2 long isoform
NM_005069.6	NP_005060.1	single-minded homolog 2 long isoform
XM_011529694.1	XP_011527996.1	single-minded homolog 2 isoform X1
NM_009586.4	NP_033664.2	single-minded homolog 2 short isoform
NM_009586.3	NP_033664.2	single-minded homolog 2 short isoform

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SIM2	XP_002685213.2
Gallus gallus (chicken)	SIM2	XP_004934598.1
Xenopus tropicalis (tropical clawed frog)	sim2	XP_002941575.2
	SIM2	XP_001085660.1
Canis lupus familiaris (dog)	SIM2	XP_005639049.1
Homo sapiens (human)	SIM2	NP_005060.1
Rattus norvegicus (Norway rat)	Sim2	NP_001100578.1
Danio rerio (zebrafish)	sim2	NP_571911.1
Pan troglodytes (chimpanzee)	SIM2	XP_001169453.1
Mus musculus (house mouse)	Sim2	NP_035507.2

Gene Ontology
Bibliography

Related articles in PubMed

SIM2l attenuates resistance to hypoxia and tumor growth by transcriptional suppression of HIF1A in uterine cervical squamous cell carcinoma.
Nakamura K, Komatsu M, Chiwaki F, Takeda T, Kobayashi Y, Banno K, Aoki D, Yoshida T, Sasaki H
Scientific reports7(1)14574(2017 Nov)

Circulating mRNAs and miRNAs as candidate markers for the diagnosis and prognosis of prostate cancer.
Souza MF, Kuasne H, Barros-Filho MC, Cili?o HL, Marchi FA, Fuganti PE, Paschoal AR, Rogatto SR, C?lus IMS
PloS one12(9)e0184094(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Two genes, OR51E2 and SIM2, and two miRNAs, miR-200c and miR-200b, showed significant association with prostate cancer.
Title: Circulating mRNAs and miRNAs as candidate markers for the diagnosis and prognosis of prostate cancer.

In SH-SY5Y cells, ethanol exposure increased Sim2 expression in a manner similar to that of cleaved caspase 3. PKA activation was required for this. Sim2 might be involved in activating caspase 3 and ethanol-induced apoptosis in SH-SY5Y cells.
Title: Ethanol Activation of PKA Mediates Single-Minded 2 Expression in Neuronal Cells.

Data showed that SIM2 was highly expressed in human glioma tumors and cell lines and its short form might increase the invasion of glioma cells through the alteration of epithelial-mesenchymal transition.
Title: Targeting SIM2-s decreases glioma cell invasion through mesenchymal--epithelial transition.

Data suggest selected SIM1 variants exhibit poor dimerization with ARNT2 (aryl-hydrocarbon receptor nuclear translocator 2) and anomalous intracellular localization; data were used to predict spot in SIM1/SIM2 (residues 290-326) critical in function.
Title: Characterization of human variants in obesity-related SIM1 protein identifies a hot-spot for dimerization with the partner protein ARNT2.

a single peptide containing multiple SIM2 epitopes can be used to induce both a CD4 and CD8 T cell response, providing a peptide-based vaccine formulation for potential use in immunotherapy of various cancers.
Title: Immunization with a peptide containing MHC class I and II epitopes derived from the tumor antigen SIM2 induces an effective CD4 and CD8 T-cell response.

The following SIM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19422	NM_005069.6 Latest version!	Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant SIM2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu30586	NM_009586.5 Latest version!	Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant SIM2s, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu93305	XM_017028442.2 Latest version!	Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu60522	XM_011529694.1 Latest version!	Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OHu93305	XM_017028442.1	Homo sapiens single-minded family bHLH transcription factor 2 (SIM2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu19422	NM_005069.5	Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant SIM2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu30586	NM_009586.4	Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant SIM2s, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu30586	NM_009586.3	Homo sapiens single-minded family bHLH transcription factor 2 (SIM2), transcript variant SIM2s, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIM2 ( NM_005069.5 ) cDNA ORF clone, Homo sapiens(Human) -> NP_005060.1 Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant SIM2, mRNA.

Price & Availability↑

CloneID	OHu19422
Clone ID Related Accession (Same CDS sequence)	NM_005069.5 , NM_005069.6
Accession Version	NM_005069.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	single-minded homolog 2 long isoform
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM848475.1, BC052313.1, AP000697.1, U80456.2 and BC110444.2. This sequence is a reference standard in the RefSeqGene project. On Feb 7, 2017 this sequence version replaced NM_005069.4. Transcript Variant: This variant (SIM2) represents the longer transcript and encodes the longer isoform (long). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U80456.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGAGA AGTCCAAGAA TGCGGCCAAG ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTTG CCAAGCTGCT CCCGCTGCCG TCGGCCATCA CTTCGCAGCT GGACAAAGCG 
TCCATCATCC GCCTCACCAC GAGCTACCTG AAGATGCGCG CCGTCTTCCC CGAAGGTTTA 
GGAGACGCGT GGGGACAGCC GAGCCGCGCC GGGCCCCTGG ACGGCGTCGC CAAGGAGCTG 
GGATCGCACT TGCTGCAGAC TTTGGATGGA TTTGTTTTTG TGGTAGCATC TGATGGCAAA 
ATCATGTATA TATCCGAGAC CGCTTCTGTC CATTTAGGCT TATCCCAGGT GGAGCTCACG 
GGCAACAGTA TTTATGAATA CATCCATCCT TCTGACCACG ATGAGATGAC CGCTGTCCTC 
ACGGCCCACC AGCCGCTGCA CCACCACCTG CTCCAAGAGT ATGAGATAGA GAGGTCGTTC 
TTTCTTCGAA TGAAATGTGT CTTGGCGAAA AGGAACGCGG GCCTGACCTG CAGCGGATAC 
AAGGTCATCC ACTGCAGTGG CTACTTGAAG ATCAGGCAGT ATATGCTGGA CATGTCCCTG 
TACGACTCCT GCTACCAGAT TGTGGGGCTG GTGGCCGTGG GCCAGTCGCT GCCACCCAGT 
GCCATCACCG AGATCAAGCT GTACAGTAAC ATGTTCATGT TCAGGGCCAG CCTTGACCTG 
AAGCTGATAT TCCTGGATTC CAGGGTGACC GAGGTGACGG GGTACGAGCC GCAGGACCTG 
ATCGAGAAGA CCCTATACCA TCACGTGCAC GGCTGCGACG TGTTCCACCT CCGCTACGCA 
CACCACCTCC TGTTGGTGAA GGGCCAGGTC ACCACCAAGT ACTACCGGCT GCTGTCCAAG 
CGGGGCGGCT GGGTGTGGGT GCAGAGCTAC GCCACCGTGG TGCACAACAG CCGCTCGTCC 
CGGCCCCACT GCATCGTGAG TGTCAATTAT GTACTCACGG AGATTGAATA CAAGGAACTT 
CAGCTGTCCC TGGAGCAGGT GTCCACTGCC AAGTCCCAGG ACTCCTGGAG GACCGCCTTG 
TCTACCTCAC AAGAAACTAG GAAATTAGTG AAACCCAAAA ATACCAAGAT GAAGACAAAG 
CTGAGAACAA ACCCTTACCC CCCACAGCAA TACAGCTCGT TCCAAATGGA CAAACTGGAA 
TGCGGCCAGC TCGGAAACTG GAGAGCCAGT CCCCCTGCAA GCGCTGCTGC TCCTCCAGAA 
CTGCAGCCCC ACTCAGAAAG CAGTGACCTT CTGTACACGC CATCCTACAG CCTGCCCTTC 
TCCTACCATT ACGGACACTT CCCTCTGGAC TCTCACGTCT TCAGCAGCAA AAAGCCAATG 
TTGCCGGCCA AGTTCGGGCA GCCCCAAGGA TCCCCTTGTG AGGTGGCACG CTTTTTCCTG 
AGCACACTGC CAGCCAGCGG TGAATGCCAG TGGCATTATG CCAACCCCCT AGTGCCTAGC 
AGCTCGTCTC CAGCTAAAAA TCCTCCAGAG CCACCGGCGA ACACTGCTAG GCACAGCCTG 
GTGCCAAGCT ACGAAGCGCC CGCCGCCGCC GTGCGCAGGT TCGGCGAGGA CACCGCGCCC 
CCGAGCTTCC CGAGCTGCGG CCACTACCGC GAGGAGCCCG CGCTGGGCCC GGCCAAAGCC 
GCCCGCCAGG CCGCCCGGGA CGGGGCGCGG CTGGCGCTGG CCCGCGCGGC ACCCGAGTGC 
TGCGCGCCCC CGACCCCCGA GGCCCCGGGC GCGCCGGCGC AGCTGCCCTT CGTGCTGCTC 
AACTACCACC GCGTGCTGGC CCGGCGCGGA CCGCTGGGGG GCGCCGCACC CGCCGCCTCC 
GGCCTGGCCT GCGCTCCCGG CGGCCCCGAG GCGGCGACCG GCGCGCTGCG GCTCCGGCAC 
CCGAGCCCCG CCGCCACCTC CCCGCCCGGC GCGCCCCTGC CGCACTACCT GGGCGCCTCG 
GTCATCATCA CCAACGGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005060.1
CDS	627..2630
Translation	MKEKSKNAAKTRREKENGEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGLGDAWGQPSRAGPLDGVAKELGSHLLQTLDGFVFVVASDGKIMYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHQPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLYSNMFMFRASLDLKLIFLDSRVTEVTGYEPQDLIEKTLYHHVHGCDVFHLRYAHHLLLVKGQVTTKYYRLLSKRGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTEIEYKELQLSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYEAPAAAVRRFGEDTAPPSFPSCGHYREEPALGPAKAARQAARDGARLALARAAPECCAPPTPEAPGAPAQLPFVLLNYHRVLARRGPLGGAAPAASGLACAPGGPEAATGALRLRHPSPAATSPPGAPLPHYLGASVIITNGR

Target ORF information:

RefSeq Version	NM_005069.5
Organism	Homo sapiens(human)
Definition	Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant SIM2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005069.5

ORF Insert Sequence:

ATGAAGGAGA AGTCCAAGAA TGCGGCCAAG ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTTG CCAAGCTGCT CCCGCTGCCG TCGGCCATCA CTTCGCAGCT GGACAAAGCG 
TCCATCATCC GCCTCACCAC GAGCTACCTG AAGATGCGCG CCGTCTTCCC CGAAGGTTTA 
GGAGACGCGT GGGGACAGCC GAGCCGCGCC GGGCCCCTGG ACGGCGTCGC CAAGGAGCTG 
GGATCGCACT TGCTGCAGAC TTTGGATGGA TTTGTTTTTG TGGTAGCATC TGATGGCAAA 
ATCATGTATA TATCCGAGAC CGCTTCTGTC CATTTAGGCT TATCCCAGGT GGAGCTCACG 
GGCAACAGTA TTTATGAATA CATCCATCCT TCTGACCACG ATGAGATGAC CGCTGTCCTC 
ACGGCCCACC AGCCGCTGCA CCACCACCTG CTCCAAGAGT ATGAGATAGA GAGGTCGTTC 
TTTCTTCGAA TGAAATGTGT CTTGGCGAAA AGGAACGCGG GCCTGACCTG CAGCGGATAC 
AAGGTCATCC ACTGCAGTGG CTACTTGAAG ATCAGGCAGT ATATGCTGGA CATGTCCCTG 
TACGACTCCT GCTACCAGAT TGTGGGGCTG GTGGCCGTGG GCCAGTCGCT GCCACCCAGT 
GCCATCACCG AGATCAAGCT GTACAGTAAC ATGTTCATGT TCAGGGCCAG CCTTGACCTG 
AAGCTGATAT TCCTGGATTC CAGGGTGACC GAGGTGACGG GGTACGAGCC GCAGGACCTG 
ATCGAGAAGA CCCTATACCA TCACGTGCAC GGCTGCGACG TGTTCCACCT CCGCTACGCA 
CACCACCTCC TGTTGGTGAA GGGCCAGGTC ACCACCAAGT ACTACCGGCT GCTGTCCAAG 
CGGGGCGGCT GGGTGTGGGT GCAGAGCTAC GCCACCGTGG TGCACAACAG CCGCTCGTCC 
CGGCCCCACT GCATCGTGAG TGTCAATTAT GTACTCACGG AGATTGAATA CAAGGAACTT 
CAGCTGTCCC TGGAGCAGGT GTCCACTGCC AAGTCCCAGG ACTCCTGGAG GACCGCCTTG 
TCTACCTCAC AAGAAACTAG GAAATTAGTG AAACCCAAAA ATACCAAGAT GAAGACAAAG 
CTGAGAACAA ACCCTTACCC CCCACAGCAA TACAGCTCGT TCCAAATGGA CAAACTGGAA 
TGCGGCCAGC TCGGAAACTG GAGAGCCAGT CCCCCTGCAA GCGCTGCTGC TCCTCCAGAA 
CTGCAGCCCC ACTCAGAAAG CAGTGACCTT CTGTACACGC CATCCTACAG CCTGCCCTTC 
TCCTACCATT ACGGACACTT CCCTCTGGAC TCTCACGTCT TCAGCAGCAA AAAGCCAATG 
TTGCCGGCCA AGTTCGGGCA GCCCCAAGGA TCCCCTTGTG AGGTGGCACG CTTTTTCCTG 
AGCACACTGC CAGCCAGCGG TGAATGCCAG TGGCATTATG CCAACCCCCT AGTGCCTAGC 
AGCTCGTCTC CAGCTAAAAA TCCTCCAGAG CCACCGGCGA ACACTGCTAG GCACAGCCTG 
GTGCCAAGCT ACGAAGCGCC CGCCGCCGCC GTGCGCAGGT TCGGCGAGGA CACCGCGCCC 
CCGAGCTTCC CGAGCTGCGG CCACTACCGC GAGGAGCCCG CGCTGGGCCC GGCCAAAGCC 
GCCCGCCAGG CCGCCCGGGA CGGGGCGCGG CTGGCGCTGG CCCGCGCGGC ACCCGAGTGC 
TGCGCGCCCC CGACCCCCGA GGCCCCGGGC GCGCCGGCGC AGCTGCCCTT CGTGCTGCTC 
AACTACCACC GCGTGCTGGC CCGGCGCGGA CCGCTGGGGG GCGCCGCACC CGCCGCCTCC 
GGCCTGGCCT GCGCTCCCGG CGGCCCCGAG GCGGCGACCG GCGCGCTGCG GCTCCGGCAC 
CCGAGCCCCG CCGCCACCTC CCCGCCCGGC GCGCCCCTGC CGCACTACCT GGGCGCCTCG 
GTCATCATCA CCAACGGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIM2 ( NM_005069.6 ) cDNA ORF clone, Homo sapiens(Human) -> NP_005060.1 Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant SIM2, mRNA.

Price & Availability↑

CloneID	OHu19422
Clone ID Related Accession (Same CDS sequence)	NM_005069.5 , NM_005069.6
Accession Version	NM_005069.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	single-minded homolog 2 long isoform
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000697.1, BM848475.1, BC052313.1, U80456.2 and BC110444.2. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_005069.5. Transcript Variant: This variant (SIM2) represents the longer transcript and encodes the longer isoform (long). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U80456.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000290399.11/ ENSP00000290399.6 RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END##

ATGAAGGAGA AGTCCAAGAA TGCGGCCAAG ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTTG CCAAGCTGCT CCCGCTGCCG TCGGCCATCA CTTCGCAGCT GGACAAAGCG 
TCCATCATCC GCCTCACCAC GAGCTACCTG AAGATGCGCG CCGTCTTCCC CGAAGGTTTA 
GGAGACGCGT GGGGACAGCC GAGCCGCGCC GGGCCCCTGG ACGGCGTCGC CAAGGAGCTG 
GGATCGCACT TGCTGCAGAC TTTGGATGGA TTTGTTTTTG TGGTAGCATC TGATGGCAAA 
ATCATGTATA TATCCGAGAC CGCTTCTGTC CATTTAGGCT TATCCCAGGT GGAGCTCACG 
GGCAACAGTA TTTATGAATA CATCCATCCT TCTGACCACG ATGAGATGAC CGCTGTCCTC 
ACGGCCCACC AGCCGCTGCA CCACCACCTG CTCCAAGAGT ATGAGATAGA GAGGTCGTTC 
TTTCTTCGAA TGAAATGTGT CTTGGCGAAA AGGAACGCGG GCCTGACCTG CAGCGGATAC 
AAGGTCATCC ACTGCAGTGG CTACTTGAAG ATCAGGCAGT ATATGCTGGA CATGTCCCTG 
TACGACTCCT GCTACCAGAT TGTGGGGCTG GTGGCCGTGG GCCAGTCGCT GCCACCCAGT 
GCCATCACCG AGATCAAGCT GTACAGTAAC ATGTTCATGT TCAGGGCCAG CCTTGACCTG 
AAGCTGATAT TCCTGGATTC CAGGGTGACC GAGGTGACGG GGTACGAGCC GCAGGACCTG 
ATCGAGAAGA CCCTATACCA TCACGTGCAC GGCTGCGACG TGTTCCACCT CCGCTACGCA 
CACCACCTCC TGTTGGTGAA GGGCCAGGTC ACCACCAAGT ACTACCGGCT GCTGTCCAAG 
CGGGGCGGCT GGGTGTGGGT GCAGAGCTAC GCCACCGTGG TGCACAACAG CCGCTCGTCC 
CGGCCCCACT GCATCGTGAG TGTCAATTAT GTACTCACGG AGATTGAATA CAAGGAACTT 
CAGCTGTCCC TGGAGCAGGT GTCCACTGCC AAGTCCCAGG ACTCCTGGAG GACCGCCTTG 
TCTACCTCAC AAGAAACTAG GAAATTAGTG AAACCCAAAA ATACCAAGAT GAAGACAAAG 
CTGAGAACAA ACCCTTACCC CCCACAGCAA TACAGCTCGT TCCAAATGGA CAAACTGGAA 
TGCGGCCAGC TCGGAAACTG GAGAGCCAGT CCCCCTGCAA GCGCTGCTGC TCCTCCAGAA 
CTGCAGCCCC ACTCAGAAAG CAGTGACCTT CTGTACACGC CATCCTACAG CCTGCCCTTC 
TCCTACCATT ACGGACACTT CCCTCTGGAC TCTCACGTCT TCAGCAGCAA AAAGCCAATG 
TTGCCGGCCA AGTTCGGGCA GCCCCAAGGA TCCCCTTGTG AGGTGGCACG CTTTTTCCTG 
AGCACACTGC CAGCCAGCGG TGAATGCCAG TGGCATTATG CCAACCCCCT AGTGCCTAGC 
AGCTCGTCTC CAGCTAAAAA TCCTCCAGAG CCACCGGCGA ACACTGCTAG GCACAGCCTG 
GTGCCAAGCT ACGAAGCGCC CGCCGCCGCC GTGCGCAGGT TCGGCGAGGA CACCGCGCCC 
CCGAGCTTCC CGAGCTGCGG CCACTACCGC GAGGAGCCCG CGCTGGGCCC GGCCAAAGCC 
GCCCGCCAGG CCGCCCGGGA CGGGGCGCGG CTGGCGCTGG CCCGCGCGGC ACCCGAGTGC 
TGCGCGCCCC CGACCCCCGA GGCCCCGGGC GCGCCGGCGC AGCTGCCCTT CGTGCTGCTC 
AACTACCACC GCGTGCTGGC CCGGCGCGGA CCGCTGGGGG GCGCCGCACC CGCCGCCTCC 
GGCCTGGCCT GCGCTCCCGG CGGCCCCGAG GCGGCGACCG GCGCGCTGCG GCTCCGGCAC 
CCGAGCCCCG CCGCCACCTC CCCGCCCGGC GCGCCCCTGC CGCACTACCT GGGCGCCTCG 
GTCATCATCA CCAACGGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005060.1
CDS	633..2636
Translation	MKEKSKNAAKTRREKENGEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGLGDAWGQPSRAGPLDGVAKELGSHLLQTLDGFVFVVASDGKIMYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHQPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLYSNMFMFRASLDLKLIFLDSRVTEVTGYEPQDLIEKTLYHHVHGCDVFHLRYAHHLLLVKGQVTTKYYRLLSKRGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTEIEYKELQLSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYEAPAAAVRRFGEDTAPPSFPSCGHYREEPALGPAKAARQAARDGARLALARAAPECCAPPTPEAPGAPAQLPFVLLNYHRVLARRGPLGGAAPAASGLACAPGGPEAATGALRLRHPSPAATSPPGAPLPHYLGASVIITNGR

Target ORF information:

RefSeq Version	NM_005069.6
Organism	Homo sapiens(human)
Definition	Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant SIM2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005069.6

ORF Insert Sequence:

ATGAAGGAGA AGTCCAAGAA TGCGGCCAAG ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTTG CCAAGCTGCT CCCGCTGCCG TCGGCCATCA CTTCGCAGCT GGACAAAGCG 
TCCATCATCC GCCTCACCAC GAGCTACCTG AAGATGCGCG CCGTCTTCCC CGAAGGTTTA 
GGAGACGCGT GGGGACAGCC GAGCCGCGCC GGGCCCCTGG ACGGCGTCGC CAAGGAGCTG 
GGATCGCACT TGCTGCAGAC TTTGGATGGA TTTGTTTTTG TGGTAGCATC TGATGGCAAA 
ATCATGTATA TATCCGAGAC CGCTTCTGTC CATTTAGGCT TATCCCAGGT GGAGCTCACG 
GGCAACAGTA TTTATGAATA CATCCATCCT TCTGACCACG ATGAGATGAC CGCTGTCCTC 
ACGGCCCACC AGCCGCTGCA CCACCACCTG CTCCAAGAGT ATGAGATAGA GAGGTCGTTC 
TTTCTTCGAA TGAAATGTGT CTTGGCGAAA AGGAACGCGG GCCTGACCTG CAGCGGATAC 
AAGGTCATCC ACTGCAGTGG CTACTTGAAG ATCAGGCAGT ATATGCTGGA CATGTCCCTG 
TACGACTCCT GCTACCAGAT TGTGGGGCTG GTGGCCGTGG GCCAGTCGCT GCCACCCAGT 
GCCATCACCG AGATCAAGCT GTACAGTAAC ATGTTCATGT TCAGGGCCAG CCTTGACCTG 
AAGCTGATAT TCCTGGATTC CAGGGTGACC GAGGTGACGG GGTACGAGCC GCAGGACCTG 
ATCGAGAAGA CCCTATACCA TCACGTGCAC GGCTGCGACG TGTTCCACCT CCGCTACGCA 
CACCACCTCC TGTTGGTGAA GGGCCAGGTC ACCACCAAGT ACTACCGGCT GCTGTCCAAG 
CGGGGCGGCT GGGTGTGGGT GCAGAGCTAC GCCACCGTGG TGCACAACAG CCGCTCGTCC 
CGGCCCCACT GCATCGTGAG TGTCAATTAT GTACTCACGG AGATTGAATA CAAGGAACTT 
CAGCTGTCCC TGGAGCAGGT GTCCACTGCC AAGTCCCAGG ACTCCTGGAG GACCGCCTTG 
TCTACCTCAC AAGAAACTAG GAAATTAGTG AAACCCAAAA ATACCAAGAT GAAGACAAAG 
CTGAGAACAA ACCCTTACCC CCCACAGCAA TACAGCTCGT TCCAAATGGA CAAACTGGAA 
TGCGGCCAGC TCGGAAACTG GAGAGCCAGT CCCCCTGCAA GCGCTGCTGC TCCTCCAGAA 
CTGCAGCCCC ACTCAGAAAG CAGTGACCTT CTGTACACGC CATCCTACAG CCTGCCCTTC 
TCCTACCATT ACGGACACTT CCCTCTGGAC TCTCACGTCT TCAGCAGCAA AAAGCCAATG 
TTGCCGGCCA AGTTCGGGCA GCCCCAAGGA TCCCCTTGTG AGGTGGCACG CTTTTTCCTG 
AGCACACTGC CAGCCAGCGG TGAATGCCAG TGGCATTATG CCAACCCCCT AGTGCCTAGC 
AGCTCGTCTC CAGCTAAAAA TCCTCCAGAG CCACCGGCGA ACACTGCTAG GCACAGCCTG 
GTGCCAAGCT ACGAAGCGCC CGCCGCCGCC GTGCGCAGGT TCGGCGAGGA CACCGCGCCC 
CCGAGCTTCC CGAGCTGCGG CCACTACCGC GAGGAGCCCG CGCTGGGCCC GGCCAAAGCC 
GCCCGCCAGG CCGCCCGGGA CGGGGCGCGG CTGGCGCTGG CCCGCGCGGC ACCCGAGTGC 
TGCGCGCCCC CGACCCCCGA GGCCCCGGGC GCGCCGGCGC AGCTGCCCTT CGTGCTGCTC 
AACTACCACC GCGTGCTGGC CCGGCGCGGA CCGCTGGGGG GCGCCGCACC CGCCGCCTCC 
GGCCTGGCCT GCGCTCCCGG CGGCCCCGAG GCGGCGACCG GCGCGCTGCG GCTCCGGCAC 
CCGAGCCCCG CCGCCACCTC CCCGCCCGGC GCGCCCCTGC CGCACTACCT GGGCGCCTCG 
GTCATCATCA CCAACGGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIM2 ( NM_009586.5 ) cDNA ORF clone, Homo sapiens(Human) -> NP_033664.2 Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant SIM2s, mRNA.

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CloneID	OHu30586
Clone ID Related Accession (Same CDS sequence)	NM_009586.5 , NM_009586.4 , NM_009586.3
Accession Version	NM_009586.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Homo sapiens(human)
Product	single-minded homolog 2 short isoform
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000697.1, BM848475.1, BC052313.1, U80457.2 and BC110444.2. On May 31, 2019 this sequence version replaced NM_009586.4. Transcript Variant: This variant (SIM2s) contains alternate 3' exon structure, and it thus differs in the 3' coding region and 3' UTR, compared to variant SIM2. The encoded isoform (short) has a distinct C-terminus and is shorter than isoform long. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U80457.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGAGA AGTCCAAGAA TGCGGCCAAG ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTTG CCAAGCTGCT CCCGCTGCCG TCGGCCATCA CTTCGCAGCT GGACAAAGCG 
TCCATCATCC GCCTCACCAC GAGCTACCTG AAGATGCGCG CCGTCTTCCC CGAAGGTTTA 
GGAGACGCGT GGGGACAGCC GAGCCGCGCC GGGCCCCTGG ACGGCGTCGC CAAGGAGCTG 
GGATCGCACT TGCTGCAGAC TTTGGATGGA TTTGTTTTTG TGGTAGCATC TGATGGCAAA 
ATCATGTATA TATCCGAGAC CGCTTCTGTC CATTTAGGCT TATCCCAGGT GGAGCTCACG 
GGCAACAGTA TTTATGAATA CATCCATCCT TCTGACCACG ATGAGATGAC CGCTGTCCTC 
ACGGCCCACC AGCCGCTGCA CCACCACCTG CTCCAAGAGT ATGAGATAGA GAGGTCGTTC 
TTTCTTCGAA TGAAATGTGT CTTGGCGAAA AGGAACGCGG GCCTGACCTG CAGCGGATAC 
AAGGTCATCC ACTGCAGTGG CTACTTGAAG ATCAGGCAGT ATATGCTGGA CATGTCCCTG 
TACGACTCCT GCTACCAGAT TGTGGGGCTG GTGGCCGTGG GCCAGTCGCT GCCACCCAGT 
GCCATCACCG AGATCAAGCT GTACAGTAAC ATGTTCATGT TCAGGGCCAG CCTTGACCTG 
AAGCTGATAT TCCTGGATTC CAGGGTGACC GAGGTGACGG GGTACGAGCC GCAGGACCTG 
ATCGAGAAGA CCCTATACCA TCACGTGCAC GGCTGCGACG TGTTCCACCT CCGCTACGCA 
CACCACCTCC TGTTGGTGAA GGGCCAGGTC ACCACCAAGT ACTACCGGCT GCTGTCCAAG 
CGGGGCGGCT GGGTGTGGGT GCAGAGCTAC GCCACCGTGG TGCACAACAG CCGCTCGTCC 
CGGCCCCACT GCATCGTGAG TGTCAATTAT GTACTCACGG AGATTGAATA CAAGGAACTT 
CAGCTGTCCC TGGAGCAGGT GTCCACTGCC AAGTCCCAGG ACTCCTGGAG GACCGCCTTG 
TCTACCTCAC AAGAAACTAG GAAATTAGTG AAACCCAAAA ATACCAAGAT GAAGACAAAG 
CTGAGAACAA ACCCTTACCC CCCACAGCAA TACAGCTCGT TCCAAATGGA CAAACTGGAA 
TGCGGCCAGC TCGGAAACTG GAGAGCCAGT CCCCCTGCAA GCGCTGCTGC TCCTCCAGAA 
CTGCAGCCCC ACTCAGAAAG CAGTGACCTT CTGTACACGC CATCCTACAG CCTGCCCTTC 
TCCTACCATT ACGGACACTT CCCTCTGGAC TCTCACGTCT TCAGCAGCAA AAAGCCAATG 
TTGCCGGCCA AGTTCGGGCA GCCCCAAGGA TCCCCTTGTG AGGTGGCACG CTTTTTCCTG 
AGCACACTGC CAGCCAGCGG TGAATGCCAG TGGCATTATG CCAACCCCCT AGTGCCTAGC 
AGCTCGTCTC CAGCTAAAAA TCCTCCAGAG CCACCGGCGA ACACTGCTAG GCACAGCCTG 
GTGCCAAGCT ACGAAGGTGG GTCAGGTCTG CTCGTGGGGA AGGTGGGAGG ACTGCGCACG 
GCCGGGAGCC GAAGCAGCCA TGGCGGTGGG TGGCAGATGG AGACAGAACC CTCACGCTTT 
GGGCAAACTT GCCCTCTTTC TGCTTCTAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033664.2
CDS	633..2345
Translation	MKEKSKNAAKTRREKENGEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGLGDAWGQPSRAGPLDGVAKELGSHLLQTLDGFVFVVASDGKIMYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHQPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLYSNMFMFRASLDLKLIFLDSRVTEVTGYEPQDLIEKTLYHHVHGCDVFHLRYAHHLLLVKGQVTTKYYRLLSKRGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTEIEYKELQLSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYEGGSGLLVGKVGGLRTAGSRSSHGGGWQMETEPSRFGQTCPLSASK

Target ORF information:

RefSeq Version	NM_009586.5
Organism	Homo sapiens(human)
Definition	Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant SIM2s, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009586.5

ORF Insert Sequence:

ATGAAGGAGA AGTCCAAGAA TGCGGCCAAG ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTTG CCAAGCTGCT CCCGCTGCCG TCGGCCATCA CTTCGCAGCT GGACAAAGCG 
TCCATCATCC GCCTCACCAC GAGCTACCTG AAGATGCGCG CCGTCTTCCC CGAAGGTTTA 
GGAGACGCGT GGGGACAGCC GAGCCGCGCC GGGCCCCTGG ACGGCGTCGC CAAGGAGCTG 
GGATCGCACT TGCTGCAGAC TTTGGATGGA TTTGTTTTTG TGGTAGCATC TGATGGCAAA 
ATCATGTATA TATCCGAGAC CGCTTCTGTC CATTTAGGCT TATCCCAGGT GGAGCTCACG 
GGCAACAGTA TTTATGAATA CATCCATCCT TCTGACCACG ATGAGATGAC CGCTGTCCTC 
ACGGCCCACC AGCCGCTGCA CCACCACCTG CTCCAAGAGT ATGAGATAGA GAGGTCGTTC 
TTTCTTCGAA TGAAATGTGT CTTGGCGAAA AGGAACGCGG GCCTGACCTG CAGCGGATAC 
AAGGTCATCC ACTGCAGTGG CTACTTGAAG ATCAGGCAGT ATATGCTGGA CATGTCCCTG 
TACGACTCCT GCTACCAGAT TGTGGGGCTG GTGGCCGTGG GCCAGTCGCT GCCACCCAGT 
GCCATCACCG AGATCAAGCT GTACAGTAAC ATGTTCATGT TCAGGGCCAG CCTTGACCTG 
AAGCTGATAT TCCTGGATTC CAGGGTGACC GAGGTGACGG GGTACGAGCC GCAGGACCTG 
ATCGAGAAGA CCCTATACCA TCACGTGCAC GGCTGCGACG TGTTCCACCT CCGCTACGCA 
CACCACCTCC TGTTGGTGAA GGGCCAGGTC ACCACCAAGT ACTACCGGCT GCTGTCCAAG 
CGGGGCGGCT GGGTGTGGGT GCAGAGCTAC GCCACCGTGG TGCACAACAG CCGCTCGTCC 
CGGCCCCACT GCATCGTGAG TGTCAATTAT GTACTCACGG AGATTGAATA CAAGGAACTT 
CAGCTGTCCC TGGAGCAGGT GTCCACTGCC AAGTCCCAGG ACTCCTGGAG GACCGCCTTG 
TCTACCTCAC AAGAAACTAG GAAATTAGTG AAACCCAAAA ATACCAAGAT GAAGACAAAG 
CTGAGAACAA ACCCTTACCC CCCACAGCAA TACAGCTCGT TCCAAATGGA CAAACTGGAA 
TGCGGCCAGC TCGGAAACTG GAGAGCCAGT CCCCCTGCAA GCGCTGCTGC TCCTCCAGAA 
CTGCAGCCCC ACTCAGAAAG CAGTGACCTT CTGTACACGC CATCCTACAG CCTGCCCTTC 
TCCTACCATT ACGGACACTT CCCTCTGGAC TCTCACGTCT TCAGCAGCAA AAAGCCAATG 
TTGCCGGCCA AGTTCGGGCA GCCCCAAGGA TCCCCTTGTG AGGTGGCACG CTTTTTCCTG 
AGCACACTGC CAGCCAGCGG TGAATGCCAG TGGCATTATG CCAACCCCCT AGTGCCTAGC 
AGCTCGTCTC CAGCTAAAAA TCCTCCAGAG CCACCGGCGA ACACTGCTAG GCACAGCCTG 
GTGCCAAGCT ACGAAGGTGG GTCAGGTCTG CTCGTGGGGA AGGTGGGAGG ACTGCGCACG 
GCCGGGAGCC GAAGCAGCCA TGGCGGTGGG TGGCAGATGG AGACAGAACC CTCACGCTTT 
GGGCAAACTT GCCCTCTTTC TGCTTCTAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIM2 ( NM_009586.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_033664.2 Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant SIM2s, mRNA.

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CloneID	OHu30586
Clone ID Related Accession (Same CDS sequence)	NM_009586.5 , NM_009586.4 , NM_009586.3
Accession Version	NM_009586.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	single-minded homolog 2 short isoform
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM848475.1, BC052313.1, AP000697.1, U80457.2 and BC110444.2. On Feb 7, 2017 this sequence version replaced NM_009586.3. Transcript Variant: This variant (SIM2s) contains alternate 3' exon structure, and it thus differs in the 3' coding region and 3' UTR, compared to variant SIM2. The encoded isoform (short) has a distinct C-terminus and is shorter than isoform long. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U80457.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGAGA AGTCCAAGAA TGCGGCCAAG ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTTG CCAAGCTGCT CCCGCTGCCG TCGGCCATCA CTTCGCAGCT GGACAAAGCG 
TCCATCATCC GCCTCACCAC GAGCTACCTG AAGATGCGCG CCGTCTTCCC CGAAGGTTTA 
GGAGACGCGT GGGGACAGCC GAGCCGCGCC GGGCCCCTGG ACGGCGTCGC CAAGGAGCTG 
GGATCGCACT TGCTGCAGAC TTTGGATGGA TTTGTTTTTG TGGTAGCATC TGATGGCAAA 
ATCATGTATA TATCCGAGAC CGCTTCTGTC CATTTAGGCT TATCCCAGGT GGAGCTCACG 
GGCAACAGTA TTTATGAATA CATCCATCCT TCTGACCACG ATGAGATGAC CGCTGTCCTC 
ACGGCCCACC AGCCGCTGCA CCACCACCTG CTCCAAGAGT ATGAGATAGA GAGGTCGTTC 
TTTCTTCGAA TGAAATGTGT CTTGGCGAAA AGGAACGCGG GCCTGACCTG CAGCGGATAC 
AAGGTCATCC ACTGCAGTGG CTACTTGAAG ATCAGGCAGT ATATGCTGGA CATGTCCCTG 
TACGACTCCT GCTACCAGAT TGTGGGGCTG GTGGCCGTGG GCCAGTCGCT GCCACCCAGT 
GCCATCACCG AGATCAAGCT GTACAGTAAC ATGTTCATGT TCAGGGCCAG CCTTGACCTG 
AAGCTGATAT TCCTGGATTC CAGGGTGACC GAGGTGACGG GGTACGAGCC GCAGGACCTG 
ATCGAGAAGA CCCTATACCA TCACGTGCAC GGCTGCGACG TGTTCCACCT CCGCTACGCA 
CACCACCTCC TGTTGGTGAA GGGCCAGGTC ACCACCAAGT ACTACCGGCT GCTGTCCAAG 
CGGGGCGGCT GGGTGTGGGT GCAGAGCTAC GCCACCGTGG TGCACAACAG CCGCTCGTCC 
CGGCCCCACT GCATCGTGAG TGTCAATTAT GTACTCACGG AGATTGAATA CAAGGAACTT 
CAGCTGTCCC TGGAGCAGGT GTCCACTGCC AAGTCCCAGG ACTCCTGGAG GACCGCCTTG 
TCTACCTCAC AAGAAACTAG GAAATTAGTG AAACCCAAAA ATACCAAGAT GAAGACAAAG 
CTGAGAACAA ACCCTTACCC CCCACAGCAA TACAGCTCGT TCCAAATGGA CAAACTGGAA 
TGCGGCCAGC TCGGAAACTG GAGAGCCAGT CCCCCTGCAA GCGCTGCTGC TCCTCCAGAA 
CTGCAGCCCC ACTCAGAAAG CAGTGACCTT CTGTACACGC CATCCTACAG CCTGCCCTTC 
TCCTACCATT ACGGACACTT CCCTCTGGAC TCTCACGTCT TCAGCAGCAA AAAGCCAATG 
TTGCCGGCCA AGTTCGGGCA GCCCCAAGGA TCCCCTTGTG AGGTGGCACG CTTTTTCCTG 
AGCACACTGC CAGCCAGCGG TGAATGCCAG TGGCATTATG CCAACCCCCT AGTGCCTAGC 
AGCTCGTCTC CAGCTAAAAA TCCTCCAGAG CCACCGGCGA ACACTGCTAG GCACAGCCTG 
GTGCCAAGCT ACGAAGGTGG GTCAGGTCTG CTCGTGGGGA AGGTGGGAGG ACTGCGCACG 
GCCGGGAGCC GAAGCAGCCA TGGCGGTGGG TGGCAGATGG AGACAGAACC CTCACGCTTT 
GGGCAAACTT GCCCTCTTTC TGCTTCTAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033664.2
CDS	627..2339
Translation	MKEKSKNAAKTRREKENGEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGLGDAWGQPSRAGPLDGVAKELGSHLLQTLDGFVFVVASDGKIMYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHQPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLYSNMFMFRASLDLKLIFLDSRVTEVTGYEPQDLIEKTLYHHVHGCDVFHLRYAHHLLLVKGQVTTKYYRLLSKRGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTEIEYKELQLSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYEGGSGLLVGKVGGLRTAGSRSSHGGGWQMETEPSRFGQTCPLSASK

Target ORF information:

RefSeq Version	NM_009586.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant SIM2s, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009586.4

ORF Insert Sequence:

ATGAAGGAGA AGTCCAAGAA TGCGGCCAAG ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTTG CCAAGCTGCT CCCGCTGCCG TCGGCCATCA CTTCGCAGCT GGACAAAGCG 
TCCATCATCC GCCTCACCAC GAGCTACCTG AAGATGCGCG CCGTCTTCCC CGAAGGTTTA 
GGAGACGCGT GGGGACAGCC GAGCCGCGCC GGGCCCCTGG ACGGCGTCGC CAAGGAGCTG 
GGATCGCACT TGCTGCAGAC TTTGGATGGA TTTGTTTTTG TGGTAGCATC TGATGGCAAA 
ATCATGTATA TATCCGAGAC CGCTTCTGTC CATTTAGGCT TATCCCAGGT GGAGCTCACG 
GGCAACAGTA TTTATGAATA CATCCATCCT TCTGACCACG ATGAGATGAC CGCTGTCCTC 
ACGGCCCACC AGCCGCTGCA CCACCACCTG CTCCAAGAGT ATGAGATAGA GAGGTCGTTC 
TTTCTTCGAA TGAAATGTGT CTTGGCGAAA AGGAACGCGG GCCTGACCTG CAGCGGATAC 
AAGGTCATCC ACTGCAGTGG CTACTTGAAG ATCAGGCAGT ATATGCTGGA CATGTCCCTG 
TACGACTCCT GCTACCAGAT TGTGGGGCTG GTGGCCGTGG GCCAGTCGCT GCCACCCAGT 
GCCATCACCG AGATCAAGCT GTACAGTAAC ATGTTCATGT TCAGGGCCAG CCTTGACCTG 
AAGCTGATAT TCCTGGATTC CAGGGTGACC GAGGTGACGG GGTACGAGCC GCAGGACCTG 
ATCGAGAAGA CCCTATACCA TCACGTGCAC GGCTGCGACG TGTTCCACCT CCGCTACGCA 
CACCACCTCC TGTTGGTGAA GGGCCAGGTC ACCACCAAGT ACTACCGGCT GCTGTCCAAG 
CGGGGCGGCT GGGTGTGGGT GCAGAGCTAC GCCACCGTGG TGCACAACAG CCGCTCGTCC 
CGGCCCCACT GCATCGTGAG TGTCAATTAT GTACTCACGG AGATTGAATA CAAGGAACTT 
CAGCTGTCCC TGGAGCAGGT GTCCACTGCC AAGTCCCAGG ACTCCTGGAG GACCGCCTTG 
TCTACCTCAC AAGAAACTAG GAAATTAGTG AAACCCAAAA ATACCAAGAT GAAGACAAAG 
CTGAGAACAA ACCCTTACCC CCCACAGCAA TACAGCTCGT TCCAAATGGA CAAACTGGAA 
TGCGGCCAGC TCGGAAACTG GAGAGCCAGT CCCCCTGCAA GCGCTGCTGC TCCTCCAGAA 
CTGCAGCCCC ACTCAGAAAG CAGTGACCTT CTGTACACGC CATCCTACAG CCTGCCCTTC 
TCCTACCATT ACGGACACTT CCCTCTGGAC TCTCACGTCT TCAGCAGCAA AAAGCCAATG 
TTGCCGGCCA AGTTCGGGCA GCCCCAAGGA TCCCCTTGTG AGGTGGCACG CTTTTTCCTG 
AGCACACTGC CAGCCAGCGG TGAATGCCAG TGGCATTATG CCAACCCCCT AGTGCCTAGC 
AGCTCGTCTC CAGCTAAAAA TCCTCCAGAG CCACCGGCGA ACACTGCTAG GCACAGCCTG 
GTGCCAAGCT ACGAAGGTGG GTCAGGTCTG CTCGTGGGGA AGGTGGGAGG ACTGCGCACG 
GCCGGGAGCC GAAGCAGCCA TGGCGGTGGG TGGCAGATGG AGACAGAACC CTCACGCTTT 
GGGCAAACTT GCCCTCTTTC TGCTTCTAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIM2 ( NM_009586.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_033664.2 Homo sapiens single-minded family bHLH transcription factor 2 (SIM2), transcript variant SIM2s, mRNA.

Price & Availability↑

CloneID	OHu30586
Clone ID Related Accession (Same CDS sequence)	NM_009586.5 , NM_009586.4 , NM_009586.3
Accession Version	NM_009586.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-12
Organism	Homo sapiens(Human)
Product	single-minded homolog 2 short isoform
Comment

ATGAAGGAGA AGTCCAAGAA TGCGGCCAAG ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTTG CCAAGCTGCT CCCGCTGCCG TCGGCCATCA CTTCGCAGCT GGACAAAGCG 
TCCATCATCC GCCTCACCAC GAGCTACCTG AAGATGCGCG CCGTCTTCCC CGAAGGTTTA 
GGAGACGCGT GGGGACAGCC GAGCCGCGCC GGGCCCCTGG ACGGCGTCGC CAAGGAGCTG 
GGATCGCACT TGCTGCAGAC TTTGGATGGA TTTGTTTTTG TGGTAGCATC TGATGGCAAA 
ATCATGTATA TATCCGAGAC CGCTTCTGTC CATTTAGGCT TATCCCAGGT GGAGCTCACG 
GGCAACAGTA TTTATGAATA CATCCATCCT TCTGACCACG ATGAGATGAC CGCTGTCCTC 
ACGGCCCACC AGCCGCTGCA CCACCACCTG CTCCAAGAGT ATGAGATAGA GAGGTCGTTC 
TTTCTTCGAA TGAAATGTGT CTTGGCGAAA AGGAACGCGG GCCTGACCTG CAGCGGATAC 
AAGGTCATCC ACTGCAGTGG CTACTTGAAG ATCAGGCAGT ATATGCTGGA CATGTCCCTG 
TACGACTCCT GCTACCAGAT TGTGGGGCTG GTGGCCGTGG GCCAGTCGCT GCCACCCAGT 
GCCATCACCG AGATCAAGCT GTACAGTAAC ATGTTCATGT TCAGGGCCAG CCTTGACCTG 
AAGCTGATAT TCCTGGATTC CAGGGTGACC GAGGTGACGG GGTACGAGCC GCAGGACCTG 
ATCGAGAAGA CCCTATACCA TCACGTGCAC GGCTGCGACG TGTTCCACCT CCGCTACGCA 
CACCACCTCC TGTTGGTGAA GGGCCAGGTC ACCACCAAGT ACTACCGGCT GCTGTCCAAG 
CGGGGCGGCT GGGTGTGGGT GCAGAGCTAC GCCACCGTGG TGCACAACAG CCGCTCGTCC 
CGGCCCCACT GCATCGTGAG TGTCAATTAT GTACTCACGG AGATTGAATA CAAGGAACTT 
CAGCTGTCCC TGGAGCAGGT GTCCACTGCC AAGTCCCAGG ACTCCTGGAG GACCGCCTTG 
TCTACCTCAC AAGAAACTAG GAAATTAGTG AAACCCAAAA ATACCAAGAT GAAGACAAAG 
CTGAGAACAA ACCCTTACCC CCCACAGCAA TACAGCTCGT TCCAAATGGA CAAACTGGAA 
TGCGGCCAGC TCGGAAACTG GAGAGCCAGT CCCCCTGCAA GCGCTGCTGC TCCTCCAGAA 
CTGCAGCCCC ACTCAGAAAG CAGTGACCTT CTGTACACGC CATCCTACAG CCTGCCCTTC 
TCCTACCATT ACGGACACTT CCCTCTGGAC TCTCACGTCT TCAGCAGCAA AAAGCCAATG 
TTGCCGGCCA AGTTCGGGCA GCCCCAAGGA TCCCCTTGTG AGGTGGCACG CTTTTTCCTG 
AGCACACTGC CAGCCAGCGG TGAATGCCAG TGGCATTATG CCAACCCCCT AGTGCCTAGC 
AGCTCGTCTC CAGCTAAAAA TCCTCCAGAG CCACCGGCGA ACACTGCTAG GCACAGCCTG 
GTGCCAAGCT ACGAAGGTGG GTCAGGTCTG CTCGTGGGGA AGGTGGGAGG ACTGCGCACG 
GCCGGGAGCC GAAGCAGCCA TGGCGGTGGG TGGCAGATGG AGACAGAACC CTCACGCTTT 
GGGCAAACTT GCCCTCTTTC TGCTTCTAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_033664.2

Translation

MKEKSKNAAKTRREKENGEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGLGDAWGQPSRAGPLDGVAKELGSHLLQTLDGFVFVVASDGKIMYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHQPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLYSNMFMFRASLDLKLIFLDSRVTEVTGYEPQDLIEKTLYHHVHGCDVFHLRYAHHLLLVKGQVTTKYYRLLSKRGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTEIEYKELQLSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYEGGSGLLVGKVGGLRTAGSRSSHGGGWQMETEPSRFGQTCPLSASK

Target ORF information:

RefSeq Version	NM_009586.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens single-minded family bHLH transcription factor 2 (SIM2), transcript variant SIM2s, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009586.3

ORF Insert Sequence:

ATGAAGGAGA AGTCCAAGAA TGCGGCCAAG ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTTG CCAAGCTGCT CCCGCTGCCG TCGGCCATCA CTTCGCAGCT GGACAAAGCG 
TCCATCATCC GCCTCACCAC GAGCTACCTG AAGATGCGCG CCGTCTTCCC CGAAGGTTTA 
GGAGACGCGT GGGGACAGCC GAGCCGCGCC GGGCCCCTGG ACGGCGTCGC CAAGGAGCTG 
GGATCGCACT TGCTGCAGAC TTTGGATGGA TTTGTTTTTG TGGTAGCATC TGATGGCAAA 
ATCATGTATA TATCCGAGAC CGCTTCTGTC CATTTAGGCT TATCCCAGGT GGAGCTCACG 
GGCAACAGTA TTTATGAATA CATCCATCCT TCTGACCACG ATGAGATGAC CGCTGTCCTC 
ACGGCCCACC AGCCGCTGCA CCACCACCTG CTCCAAGAGT ATGAGATAGA GAGGTCGTTC 
TTTCTTCGAA TGAAATGTGT CTTGGCGAAA AGGAACGCGG GCCTGACCTG CAGCGGATAC 
AAGGTCATCC ACTGCAGTGG CTACTTGAAG ATCAGGCAGT ATATGCTGGA CATGTCCCTG 
TACGACTCCT GCTACCAGAT TGTGGGGCTG GTGGCCGTGG GCCAGTCGCT GCCACCCAGT 
GCCATCACCG AGATCAAGCT GTACAGTAAC ATGTTCATGT TCAGGGCCAG CCTTGACCTG 
AAGCTGATAT TCCTGGATTC CAGGGTGACC GAGGTGACGG GGTACGAGCC GCAGGACCTG 
ATCGAGAAGA CCCTATACCA TCACGTGCAC GGCTGCGACG TGTTCCACCT CCGCTACGCA 
CACCACCTCC TGTTGGTGAA GGGCCAGGTC ACCACCAAGT ACTACCGGCT GCTGTCCAAG 
CGGGGCGGCT GGGTGTGGGT GCAGAGCTAC GCCACCGTGG TGCACAACAG CCGCTCGTCC 
CGGCCCCACT GCATCGTGAG TGTCAATTAT GTACTCACGG AGATTGAATA CAAGGAACTT 
CAGCTGTCCC TGGAGCAGGT GTCCACTGCC AAGTCCCAGG ACTCCTGGAG GACCGCCTTG 
TCTACCTCAC AAGAAACTAG GAAATTAGTG AAACCCAAAA ATACCAAGAT GAAGACAAAG 
CTGAGAACAA ACCCTTACCC CCCACAGCAA TACAGCTCGT TCCAAATGGA CAAACTGGAA 
TGCGGCCAGC TCGGAAACTG GAGAGCCAGT CCCCCTGCAA GCGCTGCTGC TCCTCCAGAA 
CTGCAGCCCC ACTCAGAAAG CAGTGACCTT CTGTACACGC CATCCTACAG CCTGCCCTTC 
TCCTACCATT ACGGACACTT CCCTCTGGAC TCTCACGTCT TCAGCAGCAA AAAGCCAATG 
TTGCCGGCCA AGTTCGGGCA GCCCCAAGGA TCCCCTTGTG AGGTGGCACG CTTTTTCCTG 
AGCACACTGC CAGCCAGCGG TGAATGCCAG TGGCATTATG CCAACCCCCT AGTGCCTAGC 
AGCTCGTCTC CAGCTAAAAA TCCTCCAGAG CCACCGGCGA ACACTGCTAG GCACAGCCTG 
GTGCCAAGCT ACGAAGGTGG GTCAGGTCTG CTCGTGGGGA AGGTGGGAGG ACTGCGCACG 
GCCGGGAGCC GAAGCAGCCA TGGCGGTGGG TGGCAGATGG AGACAGAACC CTCACGCTTT 
GGGCAAACTT GCCCTCTTTC TGCTTCTAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIM2 ( XM_017028442.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_016883931.1 Homo sapiens single-minded family bHLH transcription factor 2 (SIM2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu93305
Clone ID Related Accession (Same CDS sequence)	XM_017028442.1 , XM_017028442.2
Accession Version	XM_017028442.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	single-minded homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA AGTCCAAGAA TGCGGCCAAG ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTTG CCAAGCTGCT CCCGCTGCCG TCGGCCATCA CTTCGCAGCT GGACAAAGCG 
TCCATCATCC GCCTCACCAC GAGCTACCTG AAGATGCGCG CCGTCTTCCC CGAAGGTTTA 
GGAGACGCGT GGGGACAGCC GAGCCGCGCC GGGCCCCTGG ACGGCGTCGC CAAGGAGCTG 
GGATCGCACT TGCTGCAGAC TTTGGATGGA TTTGTTTTTG TGGTAGCATC TGATGGCAAA 
ATCATGTATA TATCCGAGAC CGCTTCTGTC CATTTAGGCT TATCCCAGGT GGAGCTCACG 
GGCAACAGTA TTTATGAATA CATCCATCCT TCTGACCACG ATGAGATGAC CGCTGTCCTC 
ACGGCCCACC AGCCGCTGCA CCACCACCTG CTCCAAGAGT ATGAGATAGA GAGGTCGTTC 
TTTCTTCGAA TGAAATGTGT CTTGGCGAAA AGGAACGCGG GCCTGACCTG CAGCGGATAC 
AAGGTCATCC ACTGCAGTGG CTACTTGAAG ATCAGGCAGT ATATGCTGGA CATGTCCCTG 
TACGACTCCT GCTACCAGAT TGTGGGGCTG GTGGCCGTGG GCCAGTCGCT GCCACCCAGT 
GCCATCACCG AGATCAAGCT GTACAGTAAC ATGTTCATGT TCAGGGCCAG CCTTGACCTG 
AAGCTGATAT TCCTGGATTC CAGGGTGACC GAGGTGACGG GGTACGAGCC GCAGGACCTG 
ATCGAGAAGA CCCTATACCA TCACGTGCAC GGCTGCGACG TGTTCCACCT CCGCTACGCA 
CACCACCTCC TGTTGGTGAA GGGCCAGGTC ACCACCAAGT ACTACCGGCT GCTGTCCAAG 
CGGGGCGGCT GGGTGTGGGT GCAGAGCTAC GCCACCGTGG TGCACAACAG CCGCTCGTCC 
CGGCCCCACT GCATCGGAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883931.1
CDS	631..1614
Translation	MKEKSKNAAKTRREKENGEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGLGDAWGQPSRAGPLDGVAKELGSHLLQTLDGFVFVVASDGKIMYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHQPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLYSNMFMFRASLDLKLIFLDSRVTEVTGYEPQDLIEKTLYHHVHGCDVFHLRYAHHLLLVKGQVTTKYYRLLSKRGGWVWVQSYATVVHNSRSSRPHCIGD

Target ORF information:

RefSeq Version	XM_017028442.1
Organism	Homo sapiens(human)
Definition	Homo sapiens single-minded family bHLH transcription factor 2 (SIM2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028442.1

ORF Insert Sequence:

ATGAAGGAGA AGTCCAAGAA TGCGGCCAAG ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTTG CCAAGCTGCT CCCGCTGCCG TCGGCCATCA CTTCGCAGCT GGACAAAGCG 
TCCATCATCC GCCTCACCAC GAGCTACCTG AAGATGCGCG CCGTCTTCCC CGAAGGTTTA 
GGAGACGCGT GGGGACAGCC GAGCCGCGCC GGGCCCCTGG ACGGCGTCGC CAAGGAGCTG 
GGATCGCACT TGCTGCAGAC TTTGGATGGA TTTGTTTTTG TGGTAGCATC TGATGGCAAA 
ATCATGTATA TATCCGAGAC CGCTTCTGTC CATTTAGGCT TATCCCAGGT GGAGCTCACG 
GGCAACAGTA TTTATGAATA CATCCATCCT TCTGACCACG ATGAGATGAC CGCTGTCCTC 
ACGGCCCACC AGCCGCTGCA CCACCACCTG CTCCAAGAGT ATGAGATAGA GAGGTCGTTC 
TTTCTTCGAA TGAAATGTGT CTTGGCGAAA AGGAACGCGG GCCTGACCTG CAGCGGATAC 
AAGGTCATCC ACTGCAGTGG CTACTTGAAG ATCAGGCAGT ATATGCTGGA CATGTCCCTG 
TACGACTCCT GCTACCAGAT TGTGGGGCTG GTGGCCGTGG GCCAGTCGCT GCCACCCAGT 
GCCATCACCG AGATCAAGCT GTACAGTAAC ATGTTCATGT TCAGGGCCAG CCTTGACCTG 
AAGCTGATAT TCCTGGATTC CAGGGTGACC GAGGTGACGG GGTACGAGCC GCAGGACCTG 
ATCGAGAAGA CCCTATACCA TCACGTGCAC GGCTGCGACG TGTTCCACCT CCGCTACGCA 
CACCACCTCC TGTTGGTGAA GGGCCAGGTC ACCACCAAGT ACTACCGGCT GCTGTCCAAG 
CGGGGCGGCT GGGTGTGGGT GCAGAGCTAC GCCACCGTGG TGCACAACAG CCGCTCGTCC 
CGGCCCCACT GCATCGGAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIM2 ( XM_017028442.2 ) cDNA ORF clone, Homo sapiens(Human) -> XP_016883931.1 Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant X2, mRNA.

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CloneID	OHu93305
Clone ID Related Accession (Same CDS sequence)	XM_017028442.1 , XM_017028442.2
Accession Version	XM_017028442.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	single-minded homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA AGTCCAAGAA TGCGGCCAAG ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTTG CCAAGCTGCT CCCGCTGCCG TCGGCCATCA CTTCGCAGCT GGACAAAGCG 
TCCATCATCC GCCTCACCAC GAGCTACCTG AAGATGCGCG CCGTCTTCCC CGAAGGTTTA 
GGAGACGCGT GGGGACAGCC GAGCCGCGCC GGGCCCCTGG ACGGCGTCGC CAAGGAGCTG 
GGATCGCACT TGCTGCAGAC TTTGGATGGA TTTGTTTTTG TGGTAGCATC TGATGGCAAA 
ATCATGTATA TATCCGAGAC CGCTTCTGTC CATTTAGGCT TATCCCAGGT GGAGCTCACG 
GGCAACAGTA TTTATGAATA CATCCATCCT TCTGACCACG ATGAGATGAC CGCTGTCCTC 
ACGGCCCACC AGCCGCTGCA CCACCACCTG CTCCAAGAGT ATGAGATAGA GAGGTCGTTC 
TTTCTTCGAA TGAAATGTGT CTTGGCGAAA AGGAACGCGG GCCTGACCTG CAGCGGATAC 
AAGGTCATCC ACTGCAGTGG CTACTTGAAG ATCAGGCAGT ATATGCTGGA CATGTCCCTG 
TACGACTCCT GCTACCAGAT TGTGGGGCTG GTGGCCGTGG GCCAGTCGCT GCCACCCAGT 
GCCATCACCG AGATCAAGCT GTACAGTAAC ATGTTCATGT TCAGGGCCAG CCTTGACCTG 
AAGCTGATAT TCCTGGATTC CAGGGTGACC GAGGTGACGG GGTACGAGCC GCAGGACCTG 
ATCGAGAAGA CCCTATACCA TCACGTGCAC GGCTGCGACG TGTTCCACCT CCGCTACGCA 
CACCACCTCC TGTTGGTGAA GGGCCAGGTC ACCACCAAGT ACTACCGGCT GCTGTCCAAG 
CGGGGCGGCT GGGTGTGGGT GCAGAGCTAC GCCACCGTGG TGCACAACAG CCGCTCGTCC 
CGGCCCCACT GCATCGGAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883931.1
CDS	631..1614
Translation	MKEKSKNAAKTRREKENGEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGLGDAWGQPSRAGPLDGVAKELGSHLLQTLDGFVFVVASDGKIMYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHQPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLYSNMFMFRASLDLKLIFLDSRVTEVTGYEPQDLIEKTLYHHVHGCDVFHLRYAHHLLLVKGQVTTKYYRLLSKRGGWVWVQSYATVVHNSRSSRPHCIGD

Target ORF information:

RefSeq Version	XM_017028442.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028442.2

ORF Insert Sequence:

ATGAAGGAGA AGTCCAAGAA TGCGGCCAAG ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTTG CCAAGCTGCT CCCGCTGCCG TCGGCCATCA CTTCGCAGCT GGACAAAGCG 
TCCATCATCC GCCTCACCAC GAGCTACCTG AAGATGCGCG CCGTCTTCCC CGAAGGTTTA 
GGAGACGCGT GGGGACAGCC GAGCCGCGCC GGGCCCCTGG ACGGCGTCGC CAAGGAGCTG 
GGATCGCACT TGCTGCAGAC TTTGGATGGA TTTGTTTTTG TGGTAGCATC TGATGGCAAA 
ATCATGTATA TATCCGAGAC CGCTTCTGTC CATTTAGGCT TATCCCAGGT GGAGCTCACG 
GGCAACAGTA TTTATGAATA CATCCATCCT TCTGACCACG ATGAGATGAC CGCTGTCCTC 
ACGGCCCACC AGCCGCTGCA CCACCACCTG CTCCAAGAGT ATGAGATAGA GAGGTCGTTC 
TTTCTTCGAA TGAAATGTGT CTTGGCGAAA AGGAACGCGG GCCTGACCTG CAGCGGATAC 
AAGGTCATCC ACTGCAGTGG CTACTTGAAG ATCAGGCAGT ATATGCTGGA CATGTCCCTG 
TACGACTCCT GCTACCAGAT TGTGGGGCTG GTGGCCGTGG GCCAGTCGCT GCCACCCAGT 
GCCATCACCG AGATCAAGCT GTACAGTAAC ATGTTCATGT TCAGGGCCAG CCTTGACCTG 
AAGCTGATAT TCCTGGATTC CAGGGTGACC GAGGTGACGG GGTACGAGCC GCAGGACCTG 
ATCGAGAAGA CCCTATACCA TCACGTGCAC GGCTGCGACG TGTTCCACCT CCGCTACGCA 
CACCACCTCC TGTTGGTGAA GGGCCAGGTC ACCACCAAGT ACTACCGGCT GCTGTCCAAG 
CGGGGCGGCT GGGTGTGGGT GCAGAGCTAC GCCACCGTGG TGCACAACAG CCGCTCGTCC 
CGGCCCCACT GCATCGGAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIM2 ( XM_011529694.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011527996.1 Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu60522
Clone ID Related Accession (Same CDS sequence)	XM_011529694.1
Accession Version	XM_011529694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	single-minded homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATATAT CCGAGACCGC TTCTGTCCAT TTAGGCTTAT CCCAGGTGGA GCTCACGGGC 
AACAGTATTT ATGAATACAT CCATCCTTCT GACCACGATG AGATGACCGC TGTCCTCACG 
GCCCACCAGC CGCTGCACCA CCACCTGCTC CAAGAGTATG AGATAGAGAG GTCGTTCTTT 
CTTCGAATGA AATGTGTCTT GGCGAAAAGG AACGCGGGCC TGACCTGCAG CGGATACAAG 
GTCATCCACT GCAGTGGCTA CTTGAAGATC AGGCAGTATA TGCTGGACAT GTCCCTGTAC 
GACTCCTGCT ACCAGATTGT GGGGCTGGTG GCCGTGGGCC AGTCGCTGCC ACCCAGTGCC 
ATCACCGAGA TCAAGCTGTA CAGTAACATG TTCATGTTCA GGGCCAGCCT TGACCTGAAG 
CTGATATTCC TGGATTCCAG GGTGACCGAG GTGACGGGGT ACGAGCCGCA GGACCTGATC 
GAGAAGACCC TATACCATCA CGTGCACGGC TGCGACGTGT TCCACCTCCG CTACGCACAC 
CACCTCCTGT TGGTGAAGGG CCAGGTCACC ACCAAGTACT ACCGGCTGCT GTCCAAGCGG 
GGCGGCTGGG TGTGGGTGCA GAGCTACGCC ACCGTGGTGC ACAACAGCCG CTCGTCCCGG 
CCCCACTGCA TCGTGAGTGT CAATTATGTA CTCACGGAGA TTGAATACAA GGAACTTCAG 
CTGTCCCTGG AGCAGGTGTC CACTGCCAAG TCCCAGGACT CCTGGAGGAC CGCCTTGTCT 
ACCTCACAAG AAACTAGGAA ATTAGTGAAA CCCAAAAATA CCAAGATGAA GACAAAGCTG 
AGAACAAACC CTTACCCCCC ACAGCAATAC AGCTCGTTCC AAATGGACAA ACTGGAATGC 
GGCCAGCTCG GAAACTGGAG AGCCAGTCCC CCTGCAAGCG CTGCTGCTCC TCCAGAACTG 
CAGCCCCACT CAGAAAGCAG TGACCTTCTG TACACGCCAT CCTACAGCCT GCCCTTCTCC 
TACCATTACG GACACTTCCC TCTGGACTCT CACGTCTTCA GCAGCAAAAA GCCAATGTTG 
CCGGCCAAGT TCGGGCAGCC CCAAGGATCC CCTTGTGAGG TGGCACGCTT TTTCCTGAGC 
ACACTGCCAG CCAGCGGTGA ATGCCAGTGG CATTATGCCA ACCCCCTAGT GCCTAGCAGC 
TCGTCTCCAG CTAAAAATCC TCCAGAGCCA CCGGCGAACA CTGCTAGGCA CAGCCTGGTG 
CCAAGCTACG AAGCGCCCGC CGCCGCCGTG CGCAGGTTCG GCGAGGACAC CGCGCCCCCG 
AGCTTCCCGA GCTGCGGCCA CTACCGCGAG GAGCCCGCGC TGGGCCCGGC CAAAGCCGCC 
CGCCAGGCCG CCCGGGACGG GGCGCGGCTG GCGCTGGCCC GCGCGGCACC CGAGTGCTGC 
GCGCCCCCGA CCCCCGAGGC CCCGGGCGCG CCGGCGCAGC TGCCCTTCGT GCTGCTCAAC 
TACCACCGCG TGCTGGCCCG GCGCGGACCG CTGGGGGGCG CCGCACCCGC CGCCTCCGGC 
CTGGCCTGCG CTCCCGGCGG CCCCGAGGCG GCGACCGGCG CGCTGCGGCT CCGGCACCCG 
AGCCCCGCCG CCACCTCCCC GCCCGGCGCG CCCCTGCCGC ACTACCTGGG CGCCTCGGTC 
ATCATCACCA ACGGGAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527996.1
CDS	1085..2785
Translation	MYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHQPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLYSNMFMFRASLDLKLIFLDSRVTEVTGYEPQDLIEKTLYHHVHGCDVFHLRYAHHLLLVKGQVTTKYYRLLSKRGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTEIEYKELQLSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYEAPAAAVRRFGEDTAPPSFPSCGHYREEPALGPAKAARQAARDGARLALARAAPECCAPPTPEAPGAPAQLPFVLLNYHRVLARRGPLGGAAPAASGLACAPGGPEAATGALRLRHPSPAATSPPGAPLPHYLGASVIITNGR

Target ORF information:

RefSeq Version	XM_011529694.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529694.1

ORF Insert Sequence:

ATGTATATAT CCGAGACCGC TTCTGTCCAT TTAGGCTTAT CCCAGGTGGA GCTCACGGGC 
AACAGTATTT ATGAATACAT CCATCCTTCT GACCACGATG AGATGACCGC TGTCCTCACG 
GCCCACCAGC CGCTGCACCA CCACCTGCTC CAAGAGTATG AGATAGAGAG GTCGTTCTTT 
CTTCGAATGA AATGTGTCTT GGCGAAAAGG AACGCGGGCC TGACCTGCAG CGGATACAAG 
GTCATCCACT GCAGTGGCTA CTTGAAGATC AGGCAGTATA TGCTGGACAT GTCCCTGTAC 
GACTCCTGCT ACCAGATTGT GGGGCTGGTG GCCGTGGGCC AGTCGCTGCC ACCCAGTGCC 
ATCACCGAGA TCAAGCTGTA CAGTAACATG TTCATGTTCA GGGCCAGCCT TGACCTGAAG 
CTGATATTCC TGGATTCCAG GGTGACCGAG GTGACGGGGT ACGAGCCGCA GGACCTGATC 
GAGAAGACCC TATACCATCA CGTGCACGGC TGCGACGTGT TCCACCTCCG CTACGCACAC 
CACCTCCTGT TGGTGAAGGG CCAGGTCACC ACCAAGTACT ACCGGCTGCT GTCCAAGCGG 
GGCGGCTGGG TGTGGGTGCA GAGCTACGCC ACCGTGGTGC ACAACAGCCG CTCGTCCCGG 
CCCCACTGCA TCGTGAGTGT CAATTATGTA CTCACGGAGA TTGAATACAA GGAACTTCAG 
CTGTCCCTGG AGCAGGTGTC CACTGCCAAG TCCCAGGACT CCTGGAGGAC CGCCTTGTCT 
ACCTCACAAG AAACTAGGAA ATTAGTGAAA CCCAAAAATA CCAAGATGAA GACAAAGCTG 
AGAACAAACC CTTACCCCCC ACAGCAATAC AGCTCGTTCC AAATGGACAA ACTGGAATGC 
GGCCAGCTCG GAAACTGGAG AGCCAGTCCC CCTGCAAGCG CTGCTGCTCC TCCAGAACTG 
CAGCCCCACT CAGAAAGCAG TGACCTTCTG TACACGCCAT CCTACAGCCT GCCCTTCTCC 
TACCATTACG GACACTTCCC TCTGGACTCT CACGTCTTCA GCAGCAAAAA GCCAATGTTG 
CCGGCCAAGT TCGGGCAGCC CCAAGGATCC CCTTGTGAGG TGGCACGCTT TTTCCTGAGC 
ACACTGCCAG CCAGCGGTGA ATGCCAGTGG CATTATGCCA ACCCCCTAGT GCCTAGCAGC 
TCGTCTCCAG CTAAAAATCC TCCAGAGCCA CCGGCGAACA CTGCTAGGCA CAGCCTGGTG 
CCAAGCTACG AAGCGCCCGC CGCCGCCGTG CGCAGGTTCG GCGAGGACAC CGCGCCCCCG 
AGCTTCCCGA GCTGCGGCCA CTACCGCGAG GAGCCCGCGC TGGGCCCGGC CAAAGCCGCC 
CGCCAGGCCG CCCGGGACGG GGCGCGGCTG GCGCTGGCCC GCGCGGCACC CGAGTGCTGC 
GCGCCCCCGA CCCCCGAGGC CCCGGGCGCG CCGGCGCAGC TGCCCTTCGT GCTGCTCAAC 
TACCACCGCG TGCTGGCCCG GCGCGGACCG CTGGGGGGCG CCGCACCCGC CGCCTCCGGC 
CTGGCCTGCG CTCCCGGCGG CCCCGAGGCG GCGACCGGCG CGCTGCGGCT CCGGCACCCG 
AGCCCCGCCG CCACCTCCCC GCCCGGCGCG CCCCTGCCGC ACTACCTGGG CGCCTCGGTC 
ATCATCACCA ACGGGAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SIM2l attenuates resistance to hypoxia and tumor growth by transcriptional suppression of HIF1A in uterine cervical squamous cell carcinoma.
Scientific reports7(1)14574(2017 Nov)
Nakamura K,Komatsu M,Chiwaki F,Takeda T,Kobayashi Y,Banno K,Aoki D,Yoshida T,Sasaki H

Circulating mRNAs and miRNAs as candidate markers for the diagnosis and prognosis of prostate cancer.
PloS one12(9)e0184094(2017)
Souza MF,Kuasne H,Barros-Filho MC,Cili?o HL,Marchi FA,Fuganti PE,Paschoal AR,Rogatto SR,C?lus IMS

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SIM2 cDNA ORF clone, Homo sapiens(Human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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