Sim2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sim2
Entrez Gene ID	20465
Full Name	single-minded family bHLH transcription factor 2
Synonyms	bHLHe15
General protein information	Preferred Names single-minded family bHLH transcription factor 2 Names single-minded homolog 2 SIM transcription factor mSIM single-minded 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 C4\|16 55.05 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006522966.3	XP_006523029.1	single-minded homolog 2 isoform X1
XM_017316897.1	XP_017172386.1	single-minded homolog 2 isoform X2
XM_006522967.3	XP_006523030.1	single-minded homolog 2 isoform X3
XM_006522967.4	XP_006523030.1	single-minded homolog 2 isoform X3
NM_011377.2	NP_035507.2	single-minded homolog 2
XM_017316897.2	XP_017172386.1	single-minded homolog 2 isoform X2
XM_006522966.4	XP_006523029.1	single-minded homolog 2 isoform X1
XM_006522968.3	XP_006523031.1	single-minded homolog 2 isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SIM2	XP_002685213.2
Gallus gallus (chicken)	SIM2	XP_004934598.1
Xenopus tropicalis (tropical clawed frog)	sim2	XP_002941575.2
	SIM2	XP_001085660.1
Canis lupus familiaris (dog)	SIM2	XP_005639049.1
Homo sapiens (human)	SIM2	NP_005060.1
Rattus norvegicus (Norway rat)	Sim2	NP_001100578.1
Danio rerio (zebrafish)	sim2	NP_571911.1
Pan troglodytes (chimpanzee)	SIM2	XP_001169453.1
Mus musculus (house mouse)	Sim2	NP_035507.2

Gene Ontology
Bibliography

Related articles in PubMed

A developmental transcriptomic analysis of
Sivakamasundari V, Kraus P, Sun W, Hu X, Lim SL, Prabhakar S, Lufkin T
Biology open6(2)187-199(2017 Feb)

Tbx18 Regulates the Differentiation of Periductal Smooth Muscle Stroma and the Maintenance of Epithelial Integrity in the Prostate.
Bolt CC, Negi S, Guimar?es-Camboa N, Zhang H, Troy JM, Lu X, Kispert A, Evans SM, Stubbs L
PloS one11(4)e0154413(2016)

Ethanol Activation of PKA Mediates Single-Minded 2 Expression in Neuronal Cells.
Wang X, Yang Z, Sun Y, Zhou H, Chu G, Zhang J, Meng X
Molecular neurobiology52(3)1234-1244(2015 Dec)

HSA21 Single-Minded 2 (Sim2) Binding Sites Co-Localize with Super-Enhancers and Pioneer Transcription Factors in Pluripotent Mouse ES Cells.
Letourneau A, Cobellis G, Fort A, Santoni F, Garieri M, Falconnet E, Ribaux P, Vannier A, Guipponi M, Carninci P, Borel C, Antonarakis SE
PloS one10(5)e0126475(2015)

SIM2 maintains innate host defense of the small intestine.
Chen KJ, Lizaso A, Lee YH
American journal of physiology. Gastrointestinal and liver physiology307(11)G1044-56(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ethanol induced Sim2 expression in cerebral cortices of P7 Mice via the PKA pathway.
Title: Ethanol Activation of PKA Mediates Single-Minded 2 Expression in Neuronal Cells.

Study provides evidence that SIM2 binds a specific set of enhancer elements thus explaining how SIM2 can regulate its gene network in neuronal features.
Title: HSA21 Single-Minded 2 (Sim2) Binding Sites Co-Localize with Super-Enhancers and Pioneer Transcription Factors in Pluripotent Mouse ES Cells.

Our results establish that SIM2 is a crucial regulator in controlling expression of intestinal AMPs
Title: SIM2 maintains innate host defense of the small intestine.

Sim2 prevents entry into the myogenic program by repressing MyoD transcription during limb embryonic myogenesis.
Title: Sim2 prevents entry into the myogenic program by repressing MyoD transcription during limb embryonic myogenesis.

mammary gland involution is disrupted in Sim2s overexpression and STAT3-mediated signaling and other pathways involved in the acute phase response are inhibited
Title: Singleminded-2s (Sim2s) promotes delayed involution of the mouse mammary gland through suppression of Stat3 and NF?B.

The following Sim2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sim2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19469	NM_011377.2 Latest version!	Mus musculus single-minded family bHLH transcription factor 2 (Sim2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu52706	XM_006522966.4 Latest version!	Mus musculus single-minded family bHLH transcription factor 2 (Sim2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu52707	XM_006522967.4 Latest version!	Mus musculus single-minded family bHLH transcription factor 2 (Sim2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu79280	XM_017316897.2 Latest version!	Mus musculus single-minded family bHLH transcription factor 2 (Sim2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu52707	XM_006522968.3 Latest version!	Mus musculus single-minded family bHLH transcription factor 2 (Sim2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OMu52707	XM_006522967.3	Mus musculus single-minded homolog 2 (Drosophila) (Sim2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu79280	XM_017316897.1	Mus musculus single-minded homolog 2 (Drosophila) (Sim2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu52706	XM_006522966.3	Mus musculus single-minded homolog 2 (Drosophila) (Sim2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sim2 ( NM_011377.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035507.2 Mus musculus single-minded family bHLH transcription factor 2 (Sim2), mRNA.

Price & Availability↑

CloneID	OMu19469
Clone ID Related Accession (Same CDS sequence)	NM_011377.2
Accession Version	NM_011377.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	single-minded homolog 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from D63383.1, U42554.1 and CU207273.8. On Dec 9, 2006 this sequence version replaced NM_011377.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D64135.1, U42554.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385, SAMN00849387 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGAGA AGTCCAAAAA TGCGGCCAAA ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTGG CCAAGCTGCT CCCGCTGCCT TCGGCCATCA CCTCGCAGCT GGACAAAGCG 
TCCATCATCC GACTCACCAC CAGCTACCTG AAGATGCGCG CGGTCTTCCC GGAAGGTCTA 
GGAGACGCGT GGGGACAGCC GAGCAGGACA GGGCCTCTGG ACAGCGTGGC AAAAGAGCTG 
GGATCCCATT TGCTGCAGAC TCTAGATGGA TTTGTGTTCG TGGTGGCCTC TGATGGCAAA 
ATCATGTATA TATCTGAGAC CGCTTCTGTC CATTTAGGCT TGTCCCAGGT GGAGCTCACA 
GGCAACAGTA TTTATGAATA CATACATCCT TCTGACCACG ACGAGATGAC AGCCGTACTT 
ACAGCCCACC CACCGCTCCA CCATCACCTG CTCCAAGAGT ACGAGATAGA GCGGTCTTTC 
TTTCTTCGAA TGAAGTGCGT CTTGGCAAAA CGAAACGCAG GCCTGACATG CAGTGGATAC 
AAGGTCATTC ACTGCAGCGG CTACCTAAAG ATCAGACAGT ACATGCTGGA CATGTCCCTG 
TACGACTCCT GTTACCAGAT CGTGGGGCTG GTGGCCGTGG GCCAGTCGCT ACCGCCCAGC 
GCCATCACGG AGATCAAGCT CCACAGCAAC ATGTTCATGT TCAGGGCCAG CCTTGACCTG 
AAGCTCATAT TCCTGGACTC CAGGGTGACA GAGCTTACGG GCTATGAGCC ACAAGACCTG 
ATCGAAAAGA CACTGTACCA TCATGTGCAT GGCTGTGACA CCTTCCACCT CCGATATGCA 
CACCACCTCT TGCTGGTGAA GGGCCAGGTC ACCACCAAAT ACTACCGGCT GCTGTCCAAG 
CTGGGCGGCT GGGTCTGGGT GCAGAGCTAC GCCACCGTGG TGCACAACAG CCGCTCCTCC 
CGGCCTCACT GCATCGTGAG TGTCAATTAT GTTCTCACGG ATGTTGAATA CAAGGAACTT 
CAGCTGTCTC TGGACCAGGT ATCCACCTCT AAGTCCCAGG AGTCCTGGAG AACCACCTTG 
TCTACCTCAC AAGAAACTAG GAAATCAGCT AAACCCAAAA ACACAAAGAT GAAGACAAAG 
CTGAGAACCA ACCCATATCC CCCACAGCAA TACAGCTCCT TCCAAATGGA CAAACTGGAG 
TGCAGCCAGG TGGGAAACTG GAGAACTAGT CCCCCCACAA ATGCTGTGGC TCCCCCGGAA 
CAGCAGCTCC ATTCAGAAGC CAGCGACCTT TTATATGGGC CGCCCTACAG CCTCCCCTTC 
TCCTACCATT ATGGACACTT CCCATTGGAC TCTCACGTCT TCAGCAGCAA GAAGCCAGGA 
TTGCCCGCCA AGTTCGGGCA GCCCCAAGGA TCCCCGTGTG AGGTGGCACG CTTCTTCCTG 
AGCACACTGC CAGCCAGCAG CGAATGCCAG TGGCACTGTG CCAACTCTCT AGTGCCCAGC 
AGCTCATCGC CAGCTAAAAA CCTTTCTGAG CCTTCTCCTG TGAATGCTGC CCGGCATGGT 
CTTGTGCCAA ACTATGAAGC GCCCTCCGCA GCCGCGCGCA GGTTCTGCGA GGACCCCGCG 
CCCCCAAGCT TCCCGAGCTG CGGCCACTAC CGCGAGGAGC CGGCGCTGGG CCCTGCCAAG 
GCGCCTCGCC AGGCGTCCCG GGACGCAGCC CGGCTGGCGC TGGCCCGAGC GCCCCCCGAG 
TGCTGCGCGC CGCCCGCCCC CGAGCCGCAG GCGCCGGCGC AGTTGCCCTT CGTGCTGCTC 
AACTACCACC GCGTGCTGGC GCGCCGCGGG CCTCTGGGGA GCGCTGCGCC CGGAGCCCCC 
GAGGCGGCCG GCTCGCTGAG GCCCCGACAC CCAGGCCCCG TCGCCGCCTC TGCGCCCGGC 
GCGCCCCGAC CGCATTACCT GGGCGCCTCG GTCATCATCA CCAACGGCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035507.2
CDS	557..2530
Translation	MKEKSKNAAKTRREKENGEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGLGDAWGQPSRTGPLDSVAKELGSHLLQTLDGFVFVVASDGKIMYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHPPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLHSNMFMFRASLDLKLIFLDSRVTELTGYEPQDLIEKTLYHHVHGCDTFHLRYAHHLLLVKGQVTTKYYRLLSKLGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTDVEYKELQLSLDQVSTSKSQESWRTTLSTSQETRKSAKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECSQVGNWRTSPPTNAVAPPEQQLHSEASDLLYGPPYSLPFSYHYGHFPLDSHVFSSKKPGLPAKFGQPQGSPCEVARFFLSTLPASSECQWHCANSLVPSSSSPAKNLSEPSPVNAARHGLVPNYEAPSAAARRFCEDPAPPSFPSCGHYREEPALGPAKAPRQASRDAARLALARAPPECCAPPAPEPQAPAQLPFVLLNYHRVLARRGPLGSAAPGAPEAAGSLRPRHPGPVAASAPGAPRPHYLGASVIITNGR

Target ORF information:

RefSeq Version	NM_011377.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus single-minded family bHLH transcription factor 2 (Sim2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011377.2

ORF Insert Sequence:

ATGAAGGAGA AGTCCAAAAA TGCGGCCAAA ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTGG CCAAGCTGCT CCCGCTGCCT TCGGCCATCA CCTCGCAGCT GGACAAAGCG 
TCCATCATCC GACTCACCAC CAGCTACCTG AAGATGCGCG CGGTCTTCCC GGAAGGTCTA 
GGAGACGCGT GGGGACAGCC GAGCAGGACA GGGCCTCTGG ACAGCGTGGC AAAAGAGCTG 
GGATCCCATT TGCTGCAGAC TCTAGATGGA TTTGTGTTCG TGGTGGCCTC TGATGGCAAA 
ATCATGTATA TATCTGAGAC CGCTTCTGTC CATTTAGGCT TGTCCCAGGT GGAGCTCACA 
GGCAACAGTA TTTATGAATA CATACATCCT TCTGACCACG ACGAGATGAC AGCCGTACTT 
ACAGCCCACC CACCGCTCCA CCATCACCTG CTCCAAGAGT ACGAGATAGA GCGGTCTTTC 
TTTCTTCGAA TGAAGTGCGT CTTGGCAAAA CGAAACGCAG GCCTGACATG CAGTGGATAC 
AAGGTCATTC ACTGCAGCGG CTACCTAAAG ATCAGACAGT ACATGCTGGA CATGTCCCTG 
TACGACTCCT GTTACCAGAT CGTGGGGCTG GTGGCCGTGG GCCAGTCGCT ACCGCCCAGC 
GCCATCACGG AGATCAAGCT CCACAGCAAC ATGTTCATGT TCAGGGCCAG CCTTGACCTG 
AAGCTCATAT TCCTGGACTC CAGGGTGACA GAGCTTACGG GCTATGAGCC ACAAGACCTG 
ATCGAAAAGA CACTGTACCA TCATGTGCAT GGCTGTGACA CCTTCCACCT CCGATATGCA 
CACCACCTCT TGCTGGTGAA GGGCCAGGTC ACCACCAAAT ACTACCGGCT GCTGTCCAAG 
CTGGGCGGCT GGGTCTGGGT GCAGAGCTAC GCCACCGTGG TGCACAACAG CCGCTCCTCC 
CGGCCTCACT GCATCGTGAG TGTCAATTAT GTTCTCACGG ATGTTGAATA CAAGGAACTT 
CAGCTGTCTC TGGACCAGGT ATCCACCTCT AAGTCCCAGG AGTCCTGGAG AACCACCTTG 
TCTACCTCAC AAGAAACTAG GAAATCAGCT AAACCCAAAA ACACAAAGAT GAAGACAAAG 
CTGAGAACCA ACCCATATCC CCCACAGCAA TACAGCTCCT TCCAAATGGA CAAACTGGAG 
TGCAGCCAGG TGGGAAACTG GAGAACTAGT CCCCCCACAA ATGCTGTGGC TCCCCCGGAA 
CAGCAGCTCC ATTCAGAAGC CAGCGACCTT TTATATGGGC CGCCCTACAG CCTCCCCTTC 
TCCTACCATT ATGGACACTT CCCATTGGAC TCTCACGTCT TCAGCAGCAA GAAGCCAGGA 
TTGCCCGCCA AGTTCGGGCA GCCCCAAGGA TCCCCGTGTG AGGTGGCACG CTTCTTCCTG 
AGCACACTGC CAGCCAGCAG CGAATGCCAG TGGCACTGTG CCAACTCTCT AGTGCCCAGC 
AGCTCATCGC CAGCTAAAAA CCTTTCTGAG CCTTCTCCTG TGAATGCTGC CCGGCATGGT 
CTTGTGCCAA ACTATGAAGC GCCCTCCGCA GCCGCGCGCA GGTTCTGCGA GGACCCCGCG 
CCCCCAAGCT TCCCGAGCTG CGGCCACTAC CGCGAGGAGC CGGCGCTGGG CCCTGCCAAG 
GCGCCTCGCC AGGCGTCCCG GGACGCAGCC CGGCTGGCGC TGGCCCGAGC GCCCCCCGAG 
TGCTGCGCGC CGCCCGCCCC CGAGCCGCAG GCGCCGGCGC AGTTGCCCTT CGTGCTGCTC 
AACTACCACC GCGTGCTGGC GCGCCGCGGG CCTCTGGGGA GCGCTGCGCC CGGAGCCCCC 
GAGGCGGCCG GCTCGCTGAG GCCCCGACAC CCAGGCCCCG TCGCCGCCTC TGCGCCCGGC 
GCGCCCCGAC CGCATTACCT GGGCGCCTCG GTCATCATCA CCAACGGCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sim2 ( XM_006522966.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523029.1 Mus musculus single-minded family bHLH transcription factor 2 (Sim2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu52706
Clone ID Related Accession (Same CDS sequence)	XM_006522966.3 , XM_006522966.4
Accession Version	XM_006522966.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	single-minded homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522966.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA AGTCCAAAAA TGCGGCCAAA ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTGG CCAAGCTGCT CCCGCTGCCT TCGGCCATCA CCTCGCAGCT GGACAAAGCG 
TCCATCATCC GACTCACCAC CAGCTACCTG AAGATGCGCG CGGTCTTCCC GGAAGGTGAG 
GCTGCAGGTC TAGGAGACGC GTGGGGACAG CCGAGCAGGA CAGGGCCTCT GGACAGCGTG 
GCAAAAGAGC TGGGATCCCA TTTGCTGCAG ACTCTAGATG GATTTGTGTT CGTGGTGGCC 
TCTGATGGCA AAATCATGTA TATATCTGAG ACCGCTTCTG TCCATTTAGG CTTGTCCCAG 
GTGGAGCTCA CAGGCAACAG TATTTATGAA TACATACATC CTTCTGACCA CGACGAGATG 
ACAGCCGTAC TTACAGCCCA CCCACCGCTC CACCATCACC TGCTCCAAGA GTACGAGATA 
GAGCGGTCTT TCTTTCTTCG AATGAAGTGC GTCTTGGCAA AACGAAACGC AGGCCTGACA 
TGCAGTGGAT ACAAGGTCAT TCACTGCAGC GGCTACCTAA AGATCAGACA GTACATGCTG 
GACATGTCCC TGTACGACTC CTGTTACCAG ATCGTGGGGC TGGTGGCCGT GGGCCAGTCG 
CTACCGCCCA GCGCCATCAC GGAGATCAAG CTCCACAGCA ACATGTTCAT GTTCAGGGCC 
AGCCTTGACC TGAAGCTCAT ATTCCTGGAC TCCAGGGTGA CAGAGCTTAC GGGCTATGAG 
CCACAAGACC TGATCGAAAA GACACTGTAC CATCATGTGC ATGGCTGTGA CACCTTCCAC 
CTCCGATATG CACACCACCT CTTGCTGGTG AAGGGCCAGG TCACCACCAA ATACTACCGG 
CTGCTGTCCA AGCTGGGCGG CTGGGTCTGG GTGCAGAGCT ACGCCACCGT GGTGCACAAC 
AGCCGCTCCT CCCGGCCTCA CTGCATCGTG AGTGTCAATT ATGTTCTCAC GGATGTTGAA 
TACAAGGAAC TTCAGCTGTC TCTGGACCAG GTATCCACCT CTAAGTCCCA GGAGTCCTGG 
AGAACCACCT TGTCTACCTC ACAAGAAACT AGGAAATCAG CTAAACCCAA AAACACAAAG 
ATGAAGACAA AGCTGAGAAC CAACCCATAT CCCCCACAGC AATACAGCTC CTTCCAAATG 
GACAAACTGG AGTGCAGCCA GGTGGGAAAC TGGAGAACTA GTCCCCCCAC AAATGCTGTG 
GCTCCCCCGG AACAGCAGCT CCATTCAGAA GCCAGCGACC TTTTATATGG GCCGCCCTAC 
AGCCTCCCCT TCTCCTACCA TTATGGACAC TTCCCATTGG ACTCTCACGT CTTCAGCAGC 
AAGAAGCCAG GATTGCCCGC CAAGTTCGGG CAGCCCCAAG GATCCCCGTG TGAGGTGGCA 
CGCTTCTTCC TGAGCACACT GCCAGCCAGC AGCGAATGCC AGTGGCACTG TGCCAACTCT 
CTAGTGCCCA GCAGCTCATC GCCAGCTAAA AACCTTTCTG AGCCTTCTCC TGTGAATGCT 
GCCCGGCATG GTCTTGTGCC AAACTATGAA GCGCCCTCCG CAGCCGCGCG CAGGTTCTGC 
GAGGACCCCG CGCCCCCAAG CTTCCCGAGC TGCGGCCACT ACCGCGAGGA GCCGGCGCTG 
GGCCCTGCCA AGGCGCCTCG CCAGGCGTCC CGGGACGCAG CCCGGCTGGC GCTGGCCCGA 
GCGCCCCCCG AGTGCTGCGC GCCGCCCGCC CCCGAGCCGC AGGCGCCGGC GCAGTTGCCC 
TTCGTGCTGC TCAACTACCA CCGCGTGCTG GCGCGCCGCG GGCCTCTGGG GAGCGCTGCG 
CCCGGAGCCC CCGAGGCGGC CGGCTCGCTG AGGCCCCGAC ACCCAGGCCC CGTCGCCGCC 
TCTGCGCCCG GCGCGCCCCG ACCGCATTAC CTGGGCGCCT CGGTCATCAT CACCAACGGC 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523029.1
CDS	713..2698
Translation	MKEKSKNAAKTRREKENGEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGEAAGLGDAWGQPSRTGPLDSVAKELGSHLLQTLDGFVFVVASDGKIMYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHPPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLHSNMFMFRASLDLKLIFLDSRVTELTGYEPQDLIEKTLYHHVHGCDTFHLRYAHHLLLVKGQVTTKYYRLLSKLGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTDVEYKELQLSLDQVSTSKSQESWRTTLSTSQETRKSAKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECSQVGNWRTSPPTNAVAPPEQQLHSEASDLLYGPPYSLPFSYHYGHFPLDSHVFSSKKPGLPAKFGQPQGSPCEVARFFLSTLPASSECQWHCANSLVPSSSSPAKNLSEPSPVNAARHGLVPNYEAPSAAARRFCEDPAPPSFPSCGHYREEPALGPAKAPRQASRDAARLALARAPPECCAPPAPEPQAPAQLPFVLLNYHRVLARRGPLGSAAPGAPEAAGSLRPRHPGPVAASAPGAPRPHYLGASVIITNGR

Target ORF information:

RefSeq Version	XM_006522966.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus single-minded family bHLH transcription factor 2 (Sim2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522966.4

ORF Insert Sequence:

ATGAAGGAGA AGTCCAAAAA TGCGGCCAAA ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTGG CCAAGCTGCT CCCGCTGCCT TCGGCCATCA CCTCGCAGCT GGACAAAGCG 
TCCATCATCC GACTCACCAC CAGCTACCTG AAGATGCGCG CGGTCTTCCC GGAAGGTGAG 
GCTGCAGGTC TAGGAGACGC GTGGGGACAG CCGAGCAGGA CAGGGCCTCT GGACAGCGTG 
GCAAAAGAGC TGGGATCCCA TTTGCTGCAG ACTCTAGATG GATTTGTGTT CGTGGTGGCC 
TCTGATGGCA AAATCATGTA TATATCTGAG ACCGCTTCTG TCCATTTAGG CTTGTCCCAG 
GTGGAGCTCA CAGGCAACAG TATTTATGAA TACATACATC CTTCTGACCA CGACGAGATG 
ACAGCCGTAC TTACAGCCCA CCCACCGCTC CACCATCACC TGCTCCAAGA GTACGAGATA 
GAGCGGTCTT TCTTTCTTCG AATGAAGTGC GTCTTGGCAA AACGAAACGC AGGCCTGACA 
TGCAGTGGAT ACAAGGTCAT TCACTGCAGC GGCTACCTAA AGATCAGACA GTACATGCTG 
GACATGTCCC TGTACGACTC CTGTTACCAG ATCGTGGGGC TGGTGGCCGT GGGCCAGTCG 
CTACCGCCCA GCGCCATCAC GGAGATCAAG CTCCACAGCA ACATGTTCAT GTTCAGGGCC 
AGCCTTGACC TGAAGCTCAT ATTCCTGGAC TCCAGGGTGA CAGAGCTTAC GGGCTATGAG 
CCACAAGACC TGATCGAAAA GACACTGTAC CATCATGTGC ATGGCTGTGA CACCTTCCAC 
CTCCGATATG CACACCACCT CTTGCTGGTG AAGGGCCAGG TCACCACCAA ATACTACCGG 
CTGCTGTCCA AGCTGGGCGG CTGGGTCTGG GTGCAGAGCT ACGCCACCGT GGTGCACAAC 
AGCCGCTCCT CCCGGCCTCA CTGCATCGTG AGTGTCAATT ATGTTCTCAC GGATGTTGAA 
TACAAGGAAC TTCAGCTGTC TCTGGACCAG GTATCCACCT CTAAGTCCCA GGAGTCCTGG 
AGAACCACCT TGTCTACCTC ACAAGAAACT AGGAAATCAG CTAAACCCAA AAACACAAAG 
ATGAAGACAA AGCTGAGAAC CAACCCATAT CCCCCACAGC AATACAGCTC CTTCCAAATG 
GACAAACTGG AGTGCAGCCA GGTGGGAAAC TGGAGAACTA GTCCCCCCAC AAATGCTGTG 
GCTCCCCCGG AACAGCAGCT CCATTCAGAA GCCAGCGACC TTTTATATGG GCCGCCCTAC 
AGCCTCCCCT TCTCCTACCA TTATGGACAC TTCCCATTGG ACTCTCACGT CTTCAGCAGC 
AAGAAGCCAG GATTGCCCGC CAAGTTCGGG CAGCCCCAAG GATCCCCGTG TGAGGTGGCA 
CGCTTCTTCC TGAGCACACT GCCAGCCAGC AGCGAATGCC AGTGGCACTG TGCCAACTCT 
CTAGTGCCCA GCAGCTCATC GCCAGCTAAA AACCTTTCTG AGCCTTCTCC TGTGAATGCT 
GCCCGGCATG GTCTTGTGCC AAACTATGAA GCGCCCTCCG CAGCCGCGCG CAGGTTCTGC 
GAGGACCCCG CGCCCCCAAG CTTCCCGAGC TGCGGCCACT ACCGCGAGGA GCCGGCGCTG 
GGCCCTGCCA AGGCGCCTCG CCAGGCGTCC CGGGACGCAG CCCGGCTGGC GCTGGCCCGA 
GCGCCCCCCG AGTGCTGCGC GCCGCCCGCC CCCGAGCCGC AGGCGCCGGC GCAGTTGCCC 
TTCGTGCTGC TCAACTACCA CCGCGTGCTG GCGCGCCGCG GGCCTCTGGG GAGCGCTGCG 
CCCGGAGCCC CCGAGGCGGC CGGCTCGCTG AGGCCCCGAC ACCCAGGCCC CGTCGCCGCC 
TCTGCGCCCG GCGCGCCCCG ACCGCATTAC CTGGGCGCCT CGGTCATCAT CACCAACGGC 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sim2 ( XM_006522967.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523030.1 Mus musculus single-minded family bHLH transcription factor 2 (Sim2), transcript variant X3, mRNA.

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CloneID	OMu52707
Clone ID Related Accession (Same CDS sequence)	XM_006522967.3 , XM_006522967.4 , XM_006522968.3
Accession Version	XM_006522967.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	single-minded homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522967.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATATAT CTGAGACCGC TTCTGTCCAT TTAGGCTTGT CCCAGGTGGA GCTCACAGGC 
AACAGTATTT ATGAATACAT ACATCCTTCT GACCACGACG AGATGACAGC CGTACTTACA 
GCCCACCCAC CGCTCCACCA TCACCTGCTC CAAGAGTACG AGATAGAGCG GTCTTTCTTT 
CTTCGAATGA AGTGCGTCTT GGCAAAACGA AACGCAGGCC TGACATGCAG TGGATACAAG 
GTCATTCACT GCAGCGGCTA CCTAAAGATC AGACAGTACA TGCTGGACAT GTCCCTGTAC 
GACTCCTGTT ACCAGATCGT GGGGCTGGTG GCCGTGGGCC AGTCGCTACC GCCCAGCGCC 
ATCACGGAGA TCAAGCTCCA CAGCAACATG TTCATGTTCA GGGCCAGCCT TGACCTGAAG 
CTCATATTCC TGGACTCCAG GGTGACAGAG CTTACGGGCT ATGAGCCACA AGACCTGATC 
GAAAAGACAC TGTACCATCA TGTGCATGGC TGTGACACCT TCCACCTCCG ATATGCACAC 
CACCTCTTGC TGGTGAAGGG CCAGGTCACC ACCAAATACT ACCGGCTGCT GTCCAAGCTG 
GGCGGCTGGG TCTGGGTGCA GAGCTACGCC ACCGTGGTGC ACAACAGCCG CTCCTCCCGG 
CCTCACTGCA TCGTGAGTGT CAATTATGTT CTCACGGATG TTGAATACAA GGAACTTCAG 
CTGTCTCTGG ACCAGGTATC CACCTCTAAG TCCCAGGAGT CCTGGAGAAC CACCTTGTCT 
ACCTCACAAG AAACTAGGAA ATCAGCTAAA CCCAAAAACA CAAAGATGAA GACAAAGCTG 
AGAACCAACC CATATCCCCC ACAGCAATAC AGCTCCTTCC AAATGGACAA ACTGGAGTGC 
AGCCAGGTGG GAAACTGGAG AACTAGTCCC CCCACAAATG CTGTGGCTCC CCCGGAACAG 
CAGCTCCATT CAGAAGCCAG CGACCTTTTA TATGGGCCGC CCTACAGCCT CCCCTTCTCC 
TACCATTATG GACACTTCCC ATTGGACTCT CACGTCTTCA GCAGCAAGAA GCCAGGATTG 
CCCGCCAAGT TCGGGCAGCC CCAAGGATCC CCGTGTGAGG TGGCACGCTT CTTCCTGAGC 
ACACTGCCAG CCAGCAGCGA ATGCCAGTGG CACTGTGCCA ACTCTCTAGT GCCCAGCAGC 
TCATCGCCAG CTAAAAACCT TTCTGAGCCT TCTCCTGTGA ATGCTGCCCG GCATGGTCTT 
GTGCCAAACT ATGAAGCGCC CTCCGCAGCC GCGCGCAGGT TCTGCGAGGA CCCCGCGCCC 
CCAAGCTTCC CGAGCTGCGG CCACTACCGC GAGGAGCCGG CGCTGGGCCC TGCCAAGGCG 
CCTCGCCAGG CGTCCCGGGA CGCAGCCCGG CTGGCGCTGG CCCGAGCGCC CCCCGAGTGC 
TGCGCGCCGC CCGCCCCCGA GCCGCAGGCG CCGGCGCAGT TGCCCTTCGT GCTGCTCAAC 
TACCACCGCG TGCTGGCGCG CCGCGGGCCT CTGGGGAGCG CTGCGCCCGG AGCCCCCGAG 
GCGGCCGGCT CGCTGAGGCC CCGACACCCA GGCCCCGTCG CCGCCTCTGC GCCCGGCGCG 
CCCCGACCGC ATTACCTGGG CGCCTCGGTC ATCATCACCA ACGGCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523030.1
CDS	231..1901
Translation	MYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHPPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLHSNMFMFRASLDLKLIFLDSRVTELTGYEPQDLIEKTLYHHVHGCDTFHLRYAHHLLLVKGQVTTKYYRLLSKLGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTDVEYKELQLSLDQVSTSKSQESWRTTLSTSQETRKSAKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECSQVGNWRTSPPTNAVAPPEQQLHSEASDLLYGPPYSLPFSYHYGHFPLDSHVFSSKKPGLPAKFGQPQGSPCEVARFFLSTLPASSECQWHCANSLVPSSSSPAKNLSEPSPVNAARHGLVPNYEAPSAAARRFCEDPAPPSFPSCGHYREEPALGPAKAPRQASRDAARLALARAPPECCAPPAPEPQAPAQLPFVLLNYHRVLARRGPLGSAAPGAPEAAGSLRPRHPGPVAASAPGAPRPHYLGASVIITNGR

Target ORF information:

RefSeq Version	XM_006522967.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus single-minded family bHLH transcription factor 2 (Sim2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522967.4

ORF Insert Sequence:

ATGTATATAT CTGAGACCGC TTCTGTCCAT TTAGGCTTGT CCCAGGTGGA GCTCACAGGC 
AACAGTATTT ATGAATACAT ACATCCTTCT GACCACGACG AGATGACAGC CGTACTTACA 
GCCCACCCAC CGCTCCACCA TCACCTGCTC CAAGAGTACG AGATAGAGCG GTCTTTCTTT 
CTTCGAATGA AGTGCGTCTT GGCAAAACGA AACGCAGGCC TGACATGCAG TGGATACAAG 
GTCATTCACT GCAGCGGCTA CCTAAAGATC AGACAGTACA TGCTGGACAT GTCCCTGTAC 
GACTCCTGTT ACCAGATCGT GGGGCTGGTG GCCGTGGGCC AGTCGCTACC GCCCAGCGCC 
ATCACGGAGA TCAAGCTCCA CAGCAACATG TTCATGTTCA GGGCCAGCCT TGACCTGAAG 
CTCATATTCC TGGACTCCAG GGTGACAGAG CTTACGGGCT ATGAGCCACA AGACCTGATC 
GAAAAGACAC TGTACCATCA TGTGCATGGC TGTGACACCT TCCACCTCCG ATATGCACAC 
CACCTCTTGC TGGTGAAGGG CCAGGTCACC ACCAAATACT ACCGGCTGCT GTCCAAGCTG 
GGCGGCTGGG TCTGGGTGCA GAGCTACGCC ACCGTGGTGC ACAACAGCCG CTCCTCCCGG 
CCTCACTGCA TCGTGAGTGT CAATTATGTT CTCACGGATG TTGAATACAA GGAACTTCAG 
CTGTCTCTGG ACCAGGTATC CACCTCTAAG TCCCAGGAGT CCTGGAGAAC CACCTTGTCT 
ACCTCACAAG AAACTAGGAA ATCAGCTAAA CCCAAAAACA CAAAGATGAA GACAAAGCTG 
AGAACCAACC CATATCCCCC ACAGCAATAC AGCTCCTTCC AAATGGACAA ACTGGAGTGC 
AGCCAGGTGG GAAACTGGAG AACTAGTCCC CCCACAAATG CTGTGGCTCC CCCGGAACAG 
CAGCTCCATT CAGAAGCCAG CGACCTTTTA TATGGGCCGC CCTACAGCCT CCCCTTCTCC 
TACCATTATG GACACTTCCC ATTGGACTCT CACGTCTTCA GCAGCAAGAA GCCAGGATTG 
CCCGCCAAGT TCGGGCAGCC CCAAGGATCC CCGTGTGAGG TGGCACGCTT CTTCCTGAGC 
ACACTGCCAG CCAGCAGCGA ATGCCAGTGG CACTGTGCCA ACTCTCTAGT GCCCAGCAGC 
TCATCGCCAG CTAAAAACCT TTCTGAGCCT TCTCCTGTGA ATGCTGCCCG GCATGGTCTT 
GTGCCAAACT ATGAAGCGCC CTCCGCAGCC GCGCGCAGGT TCTGCGAGGA CCCCGCGCCC 
CCAAGCTTCC CGAGCTGCGG CCACTACCGC GAGGAGCCGG CGCTGGGCCC TGCCAAGGCG 
CCTCGCCAGG CGTCCCGGGA CGCAGCCCGG CTGGCGCTGG CCCGAGCGCC CCCCGAGTGC 
TGCGCGCCGC CCGCCCCCGA GCCGCAGGCG CCGGCGCAGT TGCCCTTCGT GCTGCTCAAC 
TACCACCGCG TGCTGGCGCG CCGCGGGCCT CTGGGGAGCG CTGCGCCCGG AGCCCCCGAG 
GCGGCCGGCT CGCTGAGGCC CCGACACCCA GGCCCCGTCG CCGCCTCTGC GCCCGGCGCG 
CCCCGACCGC ATTACCTGGG CGCCTCGGTC ATCATCACCA ACGGCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sim2 ( XM_017316897.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172386.1 Mus musculus single-minded homolog 2 (Drosophila) (Sim2), transcript variant X2, mRNA.

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CloneID	OMu79280
Clone ID Related Accession (Same CDS sequence)	XM_017316897.1 , XM_017316897.2
Accession Version	XM_017316897.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	single-minded homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA AGTCCAAAAA TGCGGCCAAA ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTGG CCAAGCTGCT CCCGCTGCCT TCGGCCATCA CCTCGCAGCT GGACAAAGCG 
TCCATCATCC GACTCACCAC CAGCTACCTG AAGATGCGCG CGGTCTTCCC GGAAGGTGAG 
GCTGCAGGTC TAGGAGACGC GTGGGGACAG CCGAGCAGGA CAGGGCCTCT GGACAGCGTG 
GCAAAAGAGC TGGGATCCCA TTTGCTGCAG ACTCTAGATG GATTTGTGTT CGTGGTGGCC 
TCTGATGGCA AAATCATGTA TATATCTGAG ACCGCTTCTG TCCATTTAGG CTTGTCCCAG 
GTGGAGCTCA CAGGCAACAG TATTTATGAA TACATACATC CTTCTGACCA CGACGAGATG 
ACAGCCGTAC TTACAGCCCA CCCACCGCTC CACCATCACC TGCTCCAAGA GTACGAGATA 
GAGCGGTCTT TCTTTCTTCG AATGAAGTGC GTCTTGGCAA AACGAAACGC AGGCCTGACA 
TGCAGTGGAT ACAAGGTCAT TCACTGCAGC GGCTACCTAA AGATCAGACA GTACATGCTG 
GACATGTCCC TGTACGACTC CTGTTACCAG ATCGTGGGGC TGGTGGCCGT GGGCCAGTCG 
CTACCGCCCA GCGCCATCAC GGAGATCAAG CTCCACAGCA ACATGTTCAT GTTCAGGGCC 
AGCCTTGACC TGAAGCTCAT ATTCCTGGAC TCCAGGGTGA CAGAGCTTAC GGGCTATGAG 
CCACAAGACC TGATCGAAAA GACACTGTAC CATCATGTGC ATGGCTGTGA CACCTTCCAC 
CTCCGATATG CACACCACCT CTTGCTGGTG AAGGGCCAGG TCACCACCAA ATACTACCGG 
CTGCTGTCCA AGCTGGGCGG CTGGGTCTGG GTGCAGAGCT ACGCCACCGT GGTGCACAAC 
AGCCGCTCCT CCCGGCCTCA CTGCATCGTG AGTGTCAATT ATGTTCTCAC GGATGTTGAA 
TACAAGGAAC TTCAGCTGTC TCTGGACCAG GTATCCACCT CTAAGTCCCA GGAGTCCTGG 
AGAACCACCT TGTCTACCTC ACAAGAAACT AGGAAATCAG CTAAACCCAA AAACACAAAG 
ATGAAGACAA AGCTGAGAAC CAACCCATAT CCCCCACAGC AATACAGCTC CTTCCAAATG 
GACAAACTGG AGTGCAGCCA GGTGGGAAAC TGGAGAACTA GTCCCCCCAC AAATGCTGTG 
GCTCCCCCGG AACAGCAGCT CCATTCAGAA GCCAGCGACC TTTTATATGG GCCGCCCTAC 
AGCCTCCCCT TCTCCTACCA TTATGGACAC TTCCCATTGG ACTCTCACGT CTTCAGCAGC 
AAGAAGCCAG GATTGCCCGC CAAGTTCGGG CAGCCCCAAG GATCCCCGTG TGAGGTGGCA 
CGCTTCTTCC TGAGCACACT GCCAGCCAGC AGCGAATGCC AGTGGCACTG TGCCAACTCT 
CTAGTGCCCA GCAGCTCATC GCCAGCTAAA AACCTTTCTG AGCCTTCTCC TGTGAATGCT 
GCCCGGCATG GTCTTGTGCC AAACTATGAA GGTAGGTCCC ATTTACAGGA GGGGAAGGGC 
AGGGGTTCAG CCTCAGTAGC AGTGGGTGGT GGATGGAAAA AGAATCCTCA TGCTTTGGGC 
AAACTGGCCT CTTCTGCTTC TAAGCTGGGA TTTCTGTGCT TCCTACTGCC CATCATGGTT 
TCATGGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172386.1
CDS	626..2377
Translation	MKEKSKNAAKTRREKENGEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGEAAGLGDAWGQPSRTGPLDSVAKELGSHLLQTLDGFVFVVASDGKIMYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHPPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLHSNMFMFRASLDLKLIFLDSRVTELTGYEPQDLIEKTLYHHVHGCDTFHLRYAHHLLLVKGQVTTKYYRLLSKLGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTDVEYKELQLSLDQVSTSKSQESWRTTLSTSQETRKSAKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECSQVGNWRTSPPTNAVAPPEQQLHSEASDLLYGPPYSLPFSYHYGHFPLDSHVFSSKKPGLPAKFGQPQGSPCEVARFFLSTLPASSECQWHCANSLVPSSSSPAKNLSEPSPVNAARHGLVPNYEGRSHLQEGKGRGSASVAVGGGWKKNPHALGKLASSASKLGFLCFLLPIMVSWA

Target ORF information:

RefSeq Version	XM_017316897.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus single-minded homolog 2 (Drosophila) (Sim2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316897.1

ORF Insert Sequence:

ATGAAGGAGA AGTCCAAAAA TGCGGCCAAA ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTGG CCAAGCTGCT CCCGCTGCCT TCGGCCATCA CCTCGCAGCT GGACAAAGCG 
TCCATCATCC GACTCACCAC CAGCTACCTG AAGATGCGCG CGGTCTTCCC GGAAGGTGAG 
GCTGCAGGTC TAGGAGACGC GTGGGGACAG CCGAGCAGGA CAGGGCCTCT GGACAGCGTG 
GCAAAAGAGC TGGGATCCCA TTTGCTGCAG ACTCTAGATG GATTTGTGTT CGTGGTGGCC 
TCTGATGGCA AAATCATGTA TATATCTGAG ACCGCTTCTG TCCATTTAGG CTTGTCCCAG 
GTGGAGCTCA CAGGCAACAG TATTTATGAA TACATACATC CTTCTGACCA CGACGAGATG 
ACAGCCGTAC TTACAGCCCA CCCACCGCTC CACCATCACC TGCTCCAAGA GTACGAGATA 
GAGCGGTCTT TCTTTCTTCG AATGAAGTGC GTCTTGGCAA AACGAAACGC AGGCCTGACA 
TGCAGTGGAT ACAAGGTCAT TCACTGCAGC GGCTACCTAA AGATCAGACA GTACATGCTG 
GACATGTCCC TGTACGACTC CTGTTACCAG ATCGTGGGGC TGGTGGCCGT GGGCCAGTCG 
CTACCGCCCA GCGCCATCAC GGAGATCAAG CTCCACAGCA ACATGTTCAT GTTCAGGGCC 
AGCCTTGACC TGAAGCTCAT ATTCCTGGAC TCCAGGGTGA CAGAGCTTAC GGGCTATGAG 
CCACAAGACC TGATCGAAAA GACACTGTAC CATCATGTGC ATGGCTGTGA CACCTTCCAC 
CTCCGATATG CACACCACCT CTTGCTGGTG AAGGGCCAGG TCACCACCAA ATACTACCGG 
CTGCTGTCCA AGCTGGGCGG CTGGGTCTGG GTGCAGAGCT ACGCCACCGT GGTGCACAAC 
AGCCGCTCCT CCCGGCCTCA CTGCATCGTG AGTGTCAATT ATGTTCTCAC GGATGTTGAA 
TACAAGGAAC TTCAGCTGTC TCTGGACCAG GTATCCACCT CTAAGTCCCA GGAGTCCTGG 
AGAACCACCT TGTCTACCTC ACAAGAAACT AGGAAATCAG CTAAACCCAA AAACACAAAG 
ATGAAGACAA AGCTGAGAAC CAACCCATAT CCCCCACAGC AATACAGCTC CTTCCAAATG 
GACAAACTGG AGTGCAGCCA GGTGGGAAAC TGGAGAACTA GTCCCCCCAC AAATGCTGTG 
GCTCCCCCGG AACAGCAGCT CCATTCAGAA GCCAGCGACC TTTTATATGG GCCGCCCTAC 
AGCCTCCCCT TCTCCTACCA TTATGGACAC TTCCCATTGG ACTCTCACGT CTTCAGCAGC 
AAGAAGCCAG GATTGCCCGC CAAGTTCGGG CAGCCCCAAG GATCCCCGTG TGAGGTGGCA 
CGCTTCTTCC TGAGCACACT GCCAGCCAGC AGCGAATGCC AGTGGCACTG TGCCAACTCT 
CTAGTGCCCA GCAGCTCATC GCCAGCTAAA AACCTTTCTG AGCCTTCTCC TGTGAATGCT 
GCCCGGCATG GTCTTGTGCC AAACTATGAA GGTAGGTCCC ATTTACAGGA GGGGAAGGGC 
AGGGGTTCAG CCTCAGTAGC AGTGGGTGGT GGATGGAAAA AGAATCCTCA TGCTTTGGGC 
AAACTGGCCT CTTCTGCTTC TAAGCTGGGA TTTCTGTGCT TCCTACTGCC CATCATGGTT 
TCATGGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sim2 ( XM_017316897.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172386.1 Mus musculus single-minded family bHLH transcription factor 2 (Sim2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu79280
Clone ID Related Accession (Same CDS sequence)	XM_017316897.1 , XM_017316897.2
Accession Version	XM_017316897.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	single-minded homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316897.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA AGTCCAAAAA TGCGGCCAAA ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTGG CCAAGCTGCT CCCGCTGCCT TCGGCCATCA CCTCGCAGCT GGACAAAGCG 
TCCATCATCC GACTCACCAC CAGCTACCTG AAGATGCGCG CGGTCTTCCC GGAAGGTGAG 
GCTGCAGGTC TAGGAGACGC GTGGGGACAG CCGAGCAGGA CAGGGCCTCT GGACAGCGTG 
GCAAAAGAGC TGGGATCCCA TTTGCTGCAG ACTCTAGATG GATTTGTGTT CGTGGTGGCC 
TCTGATGGCA AAATCATGTA TATATCTGAG ACCGCTTCTG TCCATTTAGG CTTGTCCCAG 
GTGGAGCTCA CAGGCAACAG TATTTATGAA TACATACATC CTTCTGACCA CGACGAGATG 
ACAGCCGTAC TTACAGCCCA CCCACCGCTC CACCATCACC TGCTCCAAGA GTACGAGATA 
GAGCGGTCTT TCTTTCTTCG AATGAAGTGC GTCTTGGCAA AACGAAACGC AGGCCTGACA 
TGCAGTGGAT ACAAGGTCAT TCACTGCAGC GGCTACCTAA AGATCAGACA GTACATGCTG 
GACATGTCCC TGTACGACTC CTGTTACCAG ATCGTGGGGC TGGTGGCCGT GGGCCAGTCG 
CTACCGCCCA GCGCCATCAC GGAGATCAAG CTCCACAGCA ACATGTTCAT GTTCAGGGCC 
AGCCTTGACC TGAAGCTCAT ATTCCTGGAC TCCAGGGTGA CAGAGCTTAC GGGCTATGAG 
CCACAAGACC TGATCGAAAA GACACTGTAC CATCATGTGC ATGGCTGTGA CACCTTCCAC 
CTCCGATATG CACACCACCT CTTGCTGGTG AAGGGCCAGG TCACCACCAA ATACTACCGG 
CTGCTGTCCA AGCTGGGCGG CTGGGTCTGG GTGCAGAGCT ACGCCACCGT GGTGCACAAC 
AGCCGCTCCT CCCGGCCTCA CTGCATCGTG AGTGTCAATT ATGTTCTCAC GGATGTTGAA 
TACAAGGAAC TTCAGCTGTC TCTGGACCAG GTATCCACCT CTAAGTCCCA GGAGTCCTGG 
AGAACCACCT TGTCTACCTC ACAAGAAACT AGGAAATCAG CTAAACCCAA AAACACAAAG 
ATGAAGACAA AGCTGAGAAC CAACCCATAT CCCCCACAGC AATACAGCTC CTTCCAAATG 
GACAAACTGG AGTGCAGCCA GGTGGGAAAC TGGAGAACTA GTCCCCCCAC AAATGCTGTG 
GCTCCCCCGG AACAGCAGCT CCATTCAGAA GCCAGCGACC TTTTATATGG GCCGCCCTAC 
AGCCTCCCCT TCTCCTACCA TTATGGACAC TTCCCATTGG ACTCTCACGT CTTCAGCAGC 
AAGAAGCCAG GATTGCCCGC CAAGTTCGGG CAGCCCCAAG GATCCCCGTG TGAGGTGGCA 
CGCTTCTTCC TGAGCACACT GCCAGCCAGC AGCGAATGCC AGTGGCACTG TGCCAACTCT 
CTAGTGCCCA GCAGCTCATC GCCAGCTAAA AACCTTTCTG AGCCTTCTCC TGTGAATGCT 
GCCCGGCATG GTCTTGTGCC AAACTATGAA GGTAGGTCCC ATTTACAGGA GGGGAAGGGC 
AGGGGTTCAG CCTCAGTAGC AGTGGGTGGT GGATGGAAAA AGAATCCTCA TGCTTTGGGC 
AAACTGGCCT CTTCTGCTTC TAAGCTGGGA TTTCTGTGCT TCCTACTGCC CATCATGGTT 
TCATGGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172386.1
CDS	713..2464
Translation	MKEKSKNAAKTRREKENGEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGEAAGLGDAWGQPSRTGPLDSVAKELGSHLLQTLDGFVFVVASDGKIMYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHPPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLHSNMFMFRASLDLKLIFLDSRVTELTGYEPQDLIEKTLYHHVHGCDTFHLRYAHHLLLVKGQVTTKYYRLLSKLGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTDVEYKELQLSLDQVSTSKSQESWRTTLSTSQETRKSAKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECSQVGNWRTSPPTNAVAPPEQQLHSEASDLLYGPPYSLPFSYHYGHFPLDSHVFSSKKPGLPAKFGQPQGSPCEVARFFLSTLPASSECQWHCANSLVPSSSSPAKNLSEPSPVNAARHGLVPNYEGRSHLQEGKGRGSASVAVGGGWKKNPHALGKLASSASKLGFLCFLLPIMVSWA

Target ORF information:

RefSeq Version	XM_017316897.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus single-minded family bHLH transcription factor 2 (Sim2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316897.2

ORF Insert Sequence:

ATGAAGGAGA AGTCCAAAAA TGCGGCCAAA ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTGG CCAAGCTGCT CCCGCTGCCT TCGGCCATCA CCTCGCAGCT GGACAAAGCG 
TCCATCATCC GACTCACCAC CAGCTACCTG AAGATGCGCG CGGTCTTCCC GGAAGGTGAG 
GCTGCAGGTC TAGGAGACGC GTGGGGACAG CCGAGCAGGA CAGGGCCTCT GGACAGCGTG 
GCAAAAGAGC TGGGATCCCA TTTGCTGCAG ACTCTAGATG GATTTGTGTT CGTGGTGGCC 
TCTGATGGCA AAATCATGTA TATATCTGAG ACCGCTTCTG TCCATTTAGG CTTGTCCCAG 
GTGGAGCTCA CAGGCAACAG TATTTATGAA TACATACATC CTTCTGACCA CGACGAGATG 
ACAGCCGTAC TTACAGCCCA CCCACCGCTC CACCATCACC TGCTCCAAGA GTACGAGATA 
GAGCGGTCTT TCTTTCTTCG AATGAAGTGC GTCTTGGCAA AACGAAACGC AGGCCTGACA 
TGCAGTGGAT ACAAGGTCAT TCACTGCAGC GGCTACCTAA AGATCAGACA GTACATGCTG 
GACATGTCCC TGTACGACTC CTGTTACCAG ATCGTGGGGC TGGTGGCCGT GGGCCAGTCG 
CTACCGCCCA GCGCCATCAC GGAGATCAAG CTCCACAGCA ACATGTTCAT GTTCAGGGCC 
AGCCTTGACC TGAAGCTCAT ATTCCTGGAC TCCAGGGTGA CAGAGCTTAC GGGCTATGAG 
CCACAAGACC TGATCGAAAA GACACTGTAC CATCATGTGC ATGGCTGTGA CACCTTCCAC 
CTCCGATATG CACACCACCT CTTGCTGGTG AAGGGCCAGG TCACCACCAA ATACTACCGG 
CTGCTGTCCA AGCTGGGCGG CTGGGTCTGG GTGCAGAGCT ACGCCACCGT GGTGCACAAC 
AGCCGCTCCT CCCGGCCTCA CTGCATCGTG AGTGTCAATT ATGTTCTCAC GGATGTTGAA 
TACAAGGAAC TTCAGCTGTC TCTGGACCAG GTATCCACCT CTAAGTCCCA GGAGTCCTGG 
AGAACCACCT TGTCTACCTC ACAAGAAACT AGGAAATCAG CTAAACCCAA AAACACAAAG 
ATGAAGACAA AGCTGAGAAC CAACCCATAT CCCCCACAGC AATACAGCTC CTTCCAAATG 
GACAAACTGG AGTGCAGCCA GGTGGGAAAC TGGAGAACTA GTCCCCCCAC AAATGCTGTG 
GCTCCCCCGG AACAGCAGCT CCATTCAGAA GCCAGCGACC TTTTATATGG GCCGCCCTAC 
AGCCTCCCCT TCTCCTACCA TTATGGACAC TTCCCATTGG ACTCTCACGT CTTCAGCAGC 
AAGAAGCCAG GATTGCCCGC CAAGTTCGGG CAGCCCCAAG GATCCCCGTG TGAGGTGGCA 
CGCTTCTTCC TGAGCACACT GCCAGCCAGC AGCGAATGCC AGTGGCACTG TGCCAACTCT 
CTAGTGCCCA GCAGCTCATC GCCAGCTAAA AACCTTTCTG AGCCTTCTCC TGTGAATGCT 
GCCCGGCATG GTCTTGTGCC AAACTATGAA GGTAGGTCCC ATTTACAGGA GGGGAAGGGC 
AGGGGTTCAG CCTCAGTAGC AGTGGGTGGT GGATGGAAAA AGAATCCTCA TGCTTTGGGC 
AAACTGGCCT CTTCTGCTTC TAAGCTGGGA TTTCTGTGCT TCCTACTGCC CATCATGGTT 
TCATGGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sim2 ( XM_006522966.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523029.1 Mus musculus single-minded homolog 2 (Drosophila) (Sim2), transcript variant X1, mRNA.

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CloneID	OMu52706
Clone ID Related Accession (Same CDS sequence)	XM_006522966.3 , XM_006522966.4
Accession Version	XM_006522966.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	single-minded homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522966.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA AGTCCAAAAA TGCGGCCAAA ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTGG CCAAGCTGCT CCCGCTGCCT TCGGCCATCA CCTCGCAGCT GGACAAAGCG 
TCCATCATCC GACTCACCAC CAGCTACCTG AAGATGCGCG CGGTCTTCCC GGAAGGTGAG 
GCTGCAGGTC TAGGAGACGC GTGGGGACAG CCGAGCAGGA CAGGGCCTCT GGACAGCGTG 
GCAAAAGAGC TGGGATCCCA TTTGCTGCAG ACTCTAGATG GATTTGTGTT CGTGGTGGCC 
TCTGATGGCA AAATCATGTA TATATCTGAG ACCGCTTCTG TCCATTTAGG CTTGTCCCAG 
GTGGAGCTCA CAGGCAACAG TATTTATGAA TACATACATC CTTCTGACCA CGACGAGATG 
ACAGCCGTAC TTACAGCCCA CCCACCGCTC CACCATCACC TGCTCCAAGA GTACGAGATA 
GAGCGGTCTT TCTTTCTTCG AATGAAGTGC GTCTTGGCAA AACGAAACGC AGGCCTGACA 
TGCAGTGGAT ACAAGGTCAT TCACTGCAGC GGCTACCTAA AGATCAGACA GTACATGCTG 
GACATGTCCC TGTACGACTC CTGTTACCAG ATCGTGGGGC TGGTGGCCGT GGGCCAGTCG 
CTACCGCCCA GCGCCATCAC GGAGATCAAG CTCCACAGCA ACATGTTCAT GTTCAGGGCC 
AGCCTTGACC TGAAGCTCAT ATTCCTGGAC TCCAGGGTGA CAGAGCTTAC GGGCTATGAG 
CCACAAGACC TGATCGAAAA GACACTGTAC CATCATGTGC ATGGCTGTGA CACCTTCCAC 
CTCCGATATG CACACCACCT CTTGCTGGTG AAGGGCCAGG TCACCACCAA ATACTACCGG 
CTGCTGTCCA AGCTGGGCGG CTGGGTCTGG GTGCAGAGCT ACGCCACCGT GGTGCACAAC 
AGCCGCTCCT CCCGGCCTCA CTGCATCGTG AGTGTCAATT ATGTTCTCAC GGATGTTGAA 
TACAAGGAAC TTCAGCTGTC TCTGGACCAG GTATCCACCT CTAAGTCCCA GGAGTCCTGG 
AGAACCACCT TGTCTACCTC ACAAGAAACT AGGAAATCAG CTAAACCCAA AAACACAAAG 
ATGAAGACAA AGCTGAGAAC CAACCCATAT CCCCCACAGC AATACAGCTC CTTCCAAATG 
GACAAACTGG AGTGCAGCCA GGTGGGAAAC TGGAGAACTA GTCCCCCCAC AAATGCTGTG 
GCTCCCCCGG AACAGCAGCT CCATTCAGAA GCCAGCGACC TTTTATATGG GCCGCCCTAC 
AGCCTCCCCT TCTCCTACCA TTATGGACAC TTCCCATTGG ACTCTCACGT CTTCAGCAGC 
AAGAAGCCAG GATTGCCCGC CAAGTTCGGG CAGCCCCAAG GATCCCCGTG TGAGGTGGCA 
CGCTTCTTCC TGAGCACACT GCCAGCCAGC AGCGAATGCC AGTGGCACTG TGCCAACTCT 
CTAGTGCCCA GCAGCTCATC GCCAGCTAAA AACCTTTCTG AGCCTTCTCC TGTGAATGCT 
GCCCGGCATG GTCTTGTGCC AAACTATGAA GCGCCCTCCG CAGCCGCGCG CAGGTTCTGC 
GAGGACCCCG CGCCCCCAAG CTTCCCGAGC TGCGGCCACT ACCGCGAGGA GCCGGCGCTG 
GGCCCTGCCA AGGCGCCTCG CCAGGCGTCC CGGGACGCAG CCCGGCTGGC GCTGGCCCGA 
GCGCCCCCCG AGTGCTGCGC GCCGCCCGCC CCCGAGCCGC AGGCGCCGGC GCAGTTGCCC 
TTCGTGCTGC TCAACTACCA CCGCGTGCTG GCGCGCCGCG GGCCTCTGGG GAGCGCTGCG 
CCCGGAGCCC CCGAGGCGGC CGGCTCGCTG AGGCCCCGAC ACCCAGGCCC CGTCGCCGCC 
TCTGCGCCCG GCGCGCCCCG ACCGCATTAC CTGGGCGCCT CGGTCATCAT CACCAACGGC 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523029.1
CDS	620..2605
Translation	MKEKSKNAAKTRREKENGEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGEAAGLGDAWGQPSRTGPLDSVAKELGSHLLQTLDGFVFVVASDGKIMYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHPPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLHSNMFMFRASLDLKLIFLDSRVTELTGYEPQDLIEKTLYHHVHGCDTFHLRYAHHLLLVKGQVTTKYYRLLSKLGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTDVEYKELQLSLDQVSTSKSQESWRTTLSTSQETRKSAKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECSQVGNWRTSPPTNAVAPPEQQLHSEASDLLYGPPYSLPFSYHYGHFPLDSHVFSSKKPGLPAKFGQPQGSPCEVARFFLSTLPASSECQWHCANSLVPSSSSPAKNLSEPSPVNAARHGLVPNYEAPSAAARRFCEDPAPPSFPSCGHYREEPALGPAKAPRQASRDAARLALARAPPECCAPPAPEPQAPAQLPFVLLNYHRVLARRGPLGSAAPGAPEAAGSLRPRHPGPVAASAPGAPRPHYLGASVIITNGR

Target ORF information:

RefSeq Version	XM_006522966.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus single-minded homolog 2 (Drosophila) (Sim2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522966.3

ORF Insert Sequence:

ATGAAGGAGA AGTCCAAAAA TGCGGCCAAA ACCAGGAGGG AGAAGGAAAA TGGCGAGTTT 
TACGAGCTGG CCAAGCTGCT CCCGCTGCCT TCGGCCATCA CCTCGCAGCT GGACAAAGCG 
TCCATCATCC GACTCACCAC CAGCTACCTG AAGATGCGCG CGGTCTTCCC GGAAGGTGAG 
GCTGCAGGTC TAGGAGACGC GTGGGGACAG CCGAGCAGGA CAGGGCCTCT GGACAGCGTG 
GCAAAAGAGC TGGGATCCCA TTTGCTGCAG ACTCTAGATG GATTTGTGTT CGTGGTGGCC 
TCTGATGGCA AAATCATGTA TATATCTGAG ACCGCTTCTG TCCATTTAGG CTTGTCCCAG 
GTGGAGCTCA CAGGCAACAG TATTTATGAA TACATACATC CTTCTGACCA CGACGAGATG 
ACAGCCGTAC TTACAGCCCA CCCACCGCTC CACCATCACC TGCTCCAAGA GTACGAGATA 
GAGCGGTCTT TCTTTCTTCG AATGAAGTGC GTCTTGGCAA AACGAAACGC AGGCCTGACA 
TGCAGTGGAT ACAAGGTCAT TCACTGCAGC GGCTACCTAA AGATCAGACA GTACATGCTG 
GACATGTCCC TGTACGACTC CTGTTACCAG ATCGTGGGGC TGGTGGCCGT GGGCCAGTCG 
CTACCGCCCA GCGCCATCAC GGAGATCAAG CTCCACAGCA ACATGTTCAT GTTCAGGGCC 
AGCCTTGACC TGAAGCTCAT ATTCCTGGAC TCCAGGGTGA CAGAGCTTAC GGGCTATGAG 
CCACAAGACC TGATCGAAAA GACACTGTAC CATCATGTGC ATGGCTGTGA CACCTTCCAC 
CTCCGATATG CACACCACCT CTTGCTGGTG AAGGGCCAGG TCACCACCAA ATACTACCGG 
CTGCTGTCCA AGCTGGGCGG CTGGGTCTGG GTGCAGAGCT ACGCCACCGT GGTGCACAAC 
AGCCGCTCCT CCCGGCCTCA CTGCATCGTG AGTGTCAATT ATGTTCTCAC GGATGTTGAA 
TACAAGGAAC TTCAGCTGTC TCTGGACCAG GTATCCACCT CTAAGTCCCA GGAGTCCTGG 
AGAACCACCT TGTCTACCTC ACAAGAAACT AGGAAATCAG CTAAACCCAA AAACACAAAG 
ATGAAGACAA AGCTGAGAAC CAACCCATAT CCCCCACAGC AATACAGCTC CTTCCAAATG 
GACAAACTGG AGTGCAGCCA GGTGGGAAAC TGGAGAACTA GTCCCCCCAC AAATGCTGTG 
GCTCCCCCGG AACAGCAGCT CCATTCAGAA GCCAGCGACC TTTTATATGG GCCGCCCTAC 
AGCCTCCCCT TCTCCTACCA TTATGGACAC TTCCCATTGG ACTCTCACGT CTTCAGCAGC 
AAGAAGCCAG GATTGCCCGC CAAGTTCGGG CAGCCCCAAG GATCCCCGTG TGAGGTGGCA 
CGCTTCTTCC TGAGCACACT GCCAGCCAGC AGCGAATGCC AGTGGCACTG TGCCAACTCT 
CTAGTGCCCA GCAGCTCATC GCCAGCTAAA AACCTTTCTG AGCCTTCTCC TGTGAATGCT 
GCCCGGCATG GTCTTGTGCC AAACTATGAA GCGCCCTCCG CAGCCGCGCG CAGGTTCTGC 
GAGGACCCCG CGCCCCCAAG CTTCCCGAGC TGCGGCCACT ACCGCGAGGA GCCGGCGCTG 
GGCCCTGCCA AGGCGCCTCG CCAGGCGTCC CGGGACGCAG CCCGGCTGGC GCTGGCCCGA 
GCGCCCCCCG AGTGCTGCGC GCCGCCCGCC CCCGAGCCGC AGGCGCCGGC GCAGTTGCCC 
TTCGTGCTGC TCAACTACCA CCGCGTGCTG GCGCGCCGCG GGCCTCTGGG GAGCGCTGCG 
CCCGGAGCCC CCGAGGCGGC CGGCTCGCTG AGGCCCCGAC ACCCAGGCCC CGTCGCCGCC 
TCTGCGCCCG GCGCGCCCCG ACCGCATTAC CTGGGCGCCT CGGTCATCAT CACCAACGGC 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sim2 ( XM_006522967.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523030.1 Mus musculus single-minded homolog 2 (Drosophila) (Sim2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu52707
Clone ID Related Accession (Same CDS sequence)	XM_006522967.3 , XM_006522967.4 , XM_006522968.3
Accession Version	XM_006522967.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	single-minded homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522967.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATATAT CTGAGACCGC TTCTGTCCAT TTAGGCTTGT CCCAGGTGGA GCTCACAGGC 
AACAGTATTT ATGAATACAT ACATCCTTCT GACCACGACG AGATGACAGC CGTACTTACA 
GCCCACCCAC CGCTCCACCA TCACCTGCTC CAAGAGTACG AGATAGAGCG GTCTTTCTTT 
CTTCGAATGA AGTGCGTCTT GGCAAAACGA AACGCAGGCC TGACATGCAG TGGATACAAG 
GTCATTCACT GCAGCGGCTA CCTAAAGATC AGACAGTACA TGCTGGACAT GTCCCTGTAC 
GACTCCTGTT ACCAGATCGT GGGGCTGGTG GCCGTGGGCC AGTCGCTACC GCCCAGCGCC 
ATCACGGAGA TCAAGCTCCA CAGCAACATG TTCATGTTCA GGGCCAGCCT TGACCTGAAG 
CTCATATTCC TGGACTCCAG GGTGACAGAG CTTACGGGCT ATGAGCCACA AGACCTGATC 
GAAAAGACAC TGTACCATCA TGTGCATGGC TGTGACACCT TCCACCTCCG ATATGCACAC 
CACCTCTTGC TGGTGAAGGG CCAGGTCACC ACCAAATACT ACCGGCTGCT GTCCAAGCTG 
GGCGGCTGGG TCTGGGTGCA GAGCTACGCC ACCGTGGTGC ACAACAGCCG CTCCTCCCGG 
CCTCACTGCA TCGTGAGTGT CAATTATGTT CTCACGGATG TTGAATACAA GGAACTTCAG 
CTGTCTCTGG ACCAGGTATC CACCTCTAAG TCCCAGGAGT CCTGGAGAAC CACCTTGTCT 
ACCTCACAAG AAACTAGGAA ATCAGCTAAA CCCAAAAACA CAAAGATGAA GACAAAGCTG 
AGAACCAACC CATATCCCCC ACAGCAATAC AGCTCCTTCC AAATGGACAA ACTGGAGTGC 
AGCCAGGTGG GAAACTGGAG AACTAGTCCC CCCACAAATG CTGTGGCTCC CCCGGAACAG 
CAGCTCCATT CAGAAGCCAG CGACCTTTTA TATGGGCCGC CCTACAGCCT CCCCTTCTCC 
TACCATTATG GACACTTCCC ATTGGACTCT CACGTCTTCA GCAGCAAGAA GCCAGGATTG 
CCCGCCAAGT TCGGGCAGCC CCAAGGATCC CCGTGTGAGG TGGCACGCTT CTTCCTGAGC 
ACACTGCCAG CCAGCAGCGA ATGCCAGTGG CACTGTGCCA ACTCTCTAGT GCCCAGCAGC 
TCATCGCCAG CTAAAAACCT TTCTGAGCCT TCTCCTGTGA ATGCTGCCCG GCATGGTCTT 
GTGCCAAACT ATGAAGCGCC CTCCGCAGCC GCGCGCAGGT TCTGCGAGGA CCCCGCGCCC 
CCAAGCTTCC CGAGCTGCGG CCACTACCGC GAGGAGCCGG CGCTGGGCCC TGCCAAGGCG 
CCTCGCCAGG CGTCCCGGGA CGCAGCCCGG CTGGCGCTGG CCCGAGCGCC CCCCGAGTGC 
TGCGCGCCGC CCGCCCCCGA GCCGCAGGCG CCGGCGCAGT TGCCCTTCGT GCTGCTCAAC 
TACCACCGCG TGCTGGCGCG CCGCGGGCCT CTGGGGAGCG CTGCGCCCGG AGCCCCCGAG 
GCGGCCGGCT CGCTGAGGCC CCGACACCCA GGCCCCGTCG CCGCCTCTGC GCCCGGCGCG 
CCCCGACCGC ATTACCTGGG CGCCTCGGTC ATCATCACCA ACGGCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523030.1
CDS	232..1902
Translation	MYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHPPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLHSNMFMFRASLDLKLIFLDSRVTELTGYEPQDLIEKTLYHHVHGCDTFHLRYAHHLLLVKGQVTTKYYRLLSKLGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTDVEYKELQLSLDQVSTSKSQESWRTTLSTSQETRKSAKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECSQVGNWRTSPPTNAVAPPEQQLHSEASDLLYGPPYSLPFSYHYGHFPLDSHVFSSKKPGLPAKFGQPQGSPCEVARFFLSTLPASSECQWHCANSLVPSSSSPAKNLSEPSPVNAARHGLVPNYEAPSAAARRFCEDPAPPSFPSCGHYREEPALGPAKAPRQASRDAARLALARAPPECCAPPAPEPQAPAQLPFVLLNYHRVLARRGPLGSAAPGAPEAAGSLRPRHPGPVAASAPGAPRPHYLGASVIITNGR

Target ORF information:

RefSeq Version	XM_006522967.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus single-minded homolog 2 (Drosophila) (Sim2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522967.3

ORF Insert Sequence:

ATGTATATAT CTGAGACCGC TTCTGTCCAT TTAGGCTTGT CCCAGGTGGA GCTCACAGGC 
AACAGTATTT ATGAATACAT ACATCCTTCT GACCACGACG AGATGACAGC CGTACTTACA 
GCCCACCCAC CGCTCCACCA TCACCTGCTC CAAGAGTACG AGATAGAGCG GTCTTTCTTT 
CTTCGAATGA AGTGCGTCTT GGCAAAACGA AACGCAGGCC TGACATGCAG TGGATACAAG 
GTCATTCACT GCAGCGGCTA CCTAAAGATC AGACAGTACA TGCTGGACAT GTCCCTGTAC 
GACTCCTGTT ACCAGATCGT GGGGCTGGTG GCCGTGGGCC AGTCGCTACC GCCCAGCGCC 
ATCACGGAGA TCAAGCTCCA CAGCAACATG TTCATGTTCA GGGCCAGCCT TGACCTGAAG 
CTCATATTCC TGGACTCCAG GGTGACAGAG CTTACGGGCT ATGAGCCACA AGACCTGATC 
GAAAAGACAC TGTACCATCA TGTGCATGGC TGTGACACCT TCCACCTCCG ATATGCACAC 
CACCTCTTGC TGGTGAAGGG CCAGGTCACC ACCAAATACT ACCGGCTGCT GTCCAAGCTG 
GGCGGCTGGG TCTGGGTGCA GAGCTACGCC ACCGTGGTGC ACAACAGCCG CTCCTCCCGG 
CCTCACTGCA TCGTGAGTGT CAATTATGTT CTCACGGATG TTGAATACAA GGAACTTCAG 
CTGTCTCTGG ACCAGGTATC CACCTCTAAG TCCCAGGAGT CCTGGAGAAC CACCTTGTCT 
ACCTCACAAG AAACTAGGAA ATCAGCTAAA CCCAAAAACA CAAAGATGAA GACAAAGCTG 
AGAACCAACC CATATCCCCC ACAGCAATAC AGCTCCTTCC AAATGGACAA ACTGGAGTGC 
AGCCAGGTGG GAAACTGGAG AACTAGTCCC CCCACAAATG CTGTGGCTCC CCCGGAACAG 
CAGCTCCATT CAGAAGCCAG CGACCTTTTA TATGGGCCGC CCTACAGCCT CCCCTTCTCC 
TACCATTATG GACACTTCCC ATTGGACTCT CACGTCTTCA GCAGCAAGAA GCCAGGATTG 
CCCGCCAAGT TCGGGCAGCC CCAAGGATCC CCGTGTGAGG TGGCACGCTT CTTCCTGAGC 
ACACTGCCAG CCAGCAGCGA ATGCCAGTGG CACTGTGCCA ACTCTCTAGT GCCCAGCAGC 
TCATCGCCAG CTAAAAACCT TTCTGAGCCT TCTCCTGTGA ATGCTGCCCG GCATGGTCTT 
GTGCCAAACT ATGAAGCGCC CTCCGCAGCC GCGCGCAGGT TCTGCGAGGA CCCCGCGCCC 
CCAAGCTTCC CGAGCTGCGG CCACTACCGC GAGGAGCCGG CGCTGGGCCC TGCCAAGGCG 
CCTCGCCAGG CGTCCCGGGA CGCAGCCCGG CTGGCGCTGG CCCGAGCGCC CCCCGAGTGC 
TGCGCGCCGC CCGCCCCCGA GCCGCAGGCG CCGGCGCAGT TGCCCTTCGT GCTGCTCAAC 
TACCACCGCG TGCTGGCGCG CCGCGGGCCT CTGGGGAGCG CTGCGCCCGG AGCCCCCGAG 
GCGGCCGGCT CGCTGAGGCC CCGACACCCA GGCCCCGTCG CCGCCTCTGC GCCCGGCGCG 
CCCCGACCGC ATTACCTGGG CGCCTCGGTC ATCATCACCA ACGGCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sim2 ( XM_006522968.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523031.1 Mus musculus single-minded family bHLH transcription factor 2 (Sim2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu52707
Clone ID Related Accession (Same CDS sequence)	XM_006522967.3 , XM_006522967.4 , XM_006522968.3
Accession Version	XM_006522968.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	single-minded homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522968.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATATAT CTGAGACCGC TTCTGTCCAT TTAGGCTTGT CCCAGGTGGA GCTCACAGGC 
AACAGTATTT ATGAATACAT ACATCCTTCT GACCACGACG AGATGACAGC CGTACTTACA 
GCCCACCCAC CGCTCCACCA TCACCTGCTC CAAGAGTACG AGATAGAGCG GTCTTTCTTT 
CTTCGAATGA AGTGCGTCTT GGCAAAACGA AACGCAGGCC TGACATGCAG TGGATACAAG 
GTCATTCACT GCAGCGGCTA CCTAAAGATC AGACAGTACA TGCTGGACAT GTCCCTGTAC 
GACTCCTGTT ACCAGATCGT GGGGCTGGTG GCCGTGGGCC AGTCGCTACC GCCCAGCGCC 
ATCACGGAGA TCAAGCTCCA CAGCAACATG TTCATGTTCA GGGCCAGCCT TGACCTGAAG 
CTCATATTCC TGGACTCCAG GGTGACAGAG CTTACGGGCT ATGAGCCACA AGACCTGATC 
GAAAAGACAC TGTACCATCA TGTGCATGGC TGTGACACCT TCCACCTCCG ATATGCACAC 
CACCTCTTGC TGGTGAAGGG CCAGGTCACC ACCAAATACT ACCGGCTGCT GTCCAAGCTG 
GGCGGCTGGG TCTGGGTGCA GAGCTACGCC ACCGTGGTGC ACAACAGCCG CTCCTCCCGG 
CCTCACTGCA TCGTGAGTGT CAATTATGTT CTCACGGATG TTGAATACAA GGAACTTCAG 
CTGTCTCTGG ACCAGGTATC CACCTCTAAG TCCCAGGAGT CCTGGAGAAC CACCTTGTCT 
ACCTCACAAG AAACTAGGAA ATCAGCTAAA CCCAAAAACA CAAAGATGAA GACAAAGCTG 
AGAACCAACC CATATCCCCC ACAGCAATAC AGCTCCTTCC AAATGGACAA ACTGGAGTGC 
AGCCAGGTGG GAAACTGGAG AACTAGTCCC CCCACAAATG CTGTGGCTCC CCCGGAACAG 
CAGCTCCATT CAGAAGCCAG CGACCTTTTA TATGGGCCGC CCTACAGCCT CCCCTTCTCC 
TACCATTATG GACACTTCCC ATTGGACTCT CACGTCTTCA GCAGCAAGAA GCCAGGATTG 
CCCGCCAAGT TCGGGCAGCC CCAAGGATCC CCGTGTGAGG TGGCACGCTT CTTCCTGAGC 
ACACTGCCAG CCAGCAGCGA ATGCCAGTGG CACTGTGCCA ACTCTCTAGT GCCCAGCAGC 
TCATCGCCAG CTAAAAACCT TTCTGAGCCT TCTCCTGTGA ATGCTGCCCG GCATGGTCTT 
GTGCCAAACT ATGAAGCGCC CTCCGCAGCC GCGCGCAGGT TCTGCGAGGA CCCCGCGCCC 
CCAAGCTTCC CGAGCTGCGG CCACTACCGC GAGGAGCCGG CGCTGGGCCC TGCCAAGGCG 
CCTCGCCAGG CGTCCCGGGA CGCAGCCCGG CTGGCGCTGG CCCGAGCGCC CCCCGAGTGC 
TGCGCGCCGC CCGCCCCCGA GCCGCAGGCG CCGGCGCAGT TGCCCTTCGT GCTGCTCAAC 
TACCACCGCG TGCTGGCGCG CCGCGGGCCT CTGGGGAGCG CTGCGCCCGG AGCCCCCGAG 
GCGGCCGGCT CGCTGAGGCC CCGACACCCA GGCCCCGTCG CCGCCTCTGC GCCCGGCGCG 
CCCCGACCGC ATTACCTGGG CGCCTCGGTC ATCATCACCA ACGGCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523031.1
CDS	275..1945
Translation	MYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHPPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLHSNMFMFRASLDLKLIFLDSRVTELTGYEPQDLIEKTLYHHVHGCDTFHLRYAHHLLLVKGQVTTKYYRLLSKLGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTDVEYKELQLSLDQVSTSKSQESWRTTLSTSQETRKSAKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECSQVGNWRTSPPTNAVAPPEQQLHSEASDLLYGPPYSLPFSYHYGHFPLDSHVFSSKKPGLPAKFGQPQGSPCEVARFFLSTLPASSECQWHCANSLVPSSSSPAKNLSEPSPVNAARHGLVPNYEAPSAAARRFCEDPAPPSFPSCGHYREEPALGPAKAPRQASRDAARLALARAPPECCAPPAPEPQAPAQLPFVLLNYHRVLARRGPLGSAAPGAPEAAGSLRPRHPGPVAASAPGAPRPHYLGASVIITNGR

Target ORF information:

RefSeq Version	XM_006522968.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus single-minded family bHLH transcription factor 2 (Sim2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522968.3

ORF Insert Sequence:

ATGTATATAT CTGAGACCGC TTCTGTCCAT TTAGGCTTGT CCCAGGTGGA GCTCACAGGC 
AACAGTATTT ATGAATACAT ACATCCTTCT GACCACGACG AGATGACAGC CGTACTTACA 
GCCCACCCAC CGCTCCACCA TCACCTGCTC CAAGAGTACG AGATAGAGCG GTCTTTCTTT 
CTTCGAATGA AGTGCGTCTT GGCAAAACGA AACGCAGGCC TGACATGCAG TGGATACAAG 
GTCATTCACT GCAGCGGCTA CCTAAAGATC AGACAGTACA TGCTGGACAT GTCCCTGTAC 
GACTCCTGTT ACCAGATCGT GGGGCTGGTG GCCGTGGGCC AGTCGCTACC GCCCAGCGCC 
ATCACGGAGA TCAAGCTCCA CAGCAACATG TTCATGTTCA GGGCCAGCCT TGACCTGAAG 
CTCATATTCC TGGACTCCAG GGTGACAGAG CTTACGGGCT ATGAGCCACA AGACCTGATC 
GAAAAGACAC TGTACCATCA TGTGCATGGC TGTGACACCT TCCACCTCCG ATATGCACAC 
CACCTCTTGC TGGTGAAGGG CCAGGTCACC ACCAAATACT ACCGGCTGCT GTCCAAGCTG 
GGCGGCTGGG TCTGGGTGCA GAGCTACGCC ACCGTGGTGC ACAACAGCCG CTCCTCCCGG 
CCTCACTGCA TCGTGAGTGT CAATTATGTT CTCACGGATG TTGAATACAA GGAACTTCAG 
CTGTCTCTGG ACCAGGTATC CACCTCTAAG TCCCAGGAGT CCTGGAGAAC CACCTTGTCT 
ACCTCACAAG AAACTAGGAA ATCAGCTAAA CCCAAAAACA CAAAGATGAA GACAAAGCTG 
AGAACCAACC CATATCCCCC ACAGCAATAC AGCTCCTTCC AAATGGACAA ACTGGAGTGC 
AGCCAGGTGG GAAACTGGAG AACTAGTCCC CCCACAAATG CTGTGGCTCC CCCGGAACAG 
CAGCTCCATT CAGAAGCCAG CGACCTTTTA TATGGGCCGC CCTACAGCCT CCCCTTCTCC 
TACCATTATG GACACTTCCC ATTGGACTCT CACGTCTTCA GCAGCAAGAA GCCAGGATTG 
CCCGCCAAGT TCGGGCAGCC CCAAGGATCC CCGTGTGAGG TGGCACGCTT CTTCCTGAGC 
ACACTGCCAG CCAGCAGCGA ATGCCAGTGG CACTGTGCCA ACTCTCTAGT GCCCAGCAGC 
TCATCGCCAG CTAAAAACCT TTCTGAGCCT TCTCCTGTGA ATGCTGCCCG GCATGGTCTT 
GTGCCAAACT ATGAAGCGCC CTCCGCAGCC GCGCGCAGGT TCTGCGAGGA CCCCGCGCCC 
CCAAGCTTCC CGAGCTGCGG CCACTACCGC GAGGAGCCGG CGCTGGGCCC TGCCAAGGCG 
CCTCGCCAGG CGTCCCGGGA CGCAGCCCGG CTGGCGCTGG CCCGAGCGCC CCCCGAGTGC 
TGCGCGCCGC CCGCCCCCGA GCCGCAGGCG CCGGCGCAGT TGCCCTTCGT GCTGCTCAAC 
TACCACCGCG TGCTGGCGCG CCGCGGGCCT CTGGGGAGCG CTGCGCCCGG AGCCCCCGAG 
GCGGCCGGCT CGCTGAGGCC CCGACACCCA GGCCCCGTCG CCGCCTCTGC GCCCGGCGCG 
CCCCGACCGC ATTACCTGGG CGCCTCGGTC ATCATCACCA ACGGCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A developmental transcriptomic analysis of
Biology open6(2)187-199(2017 Feb)
Sivakamasundari V,Kraus P,Sun W,Hu X,Lim SL,Prabhakar S,Lufkin T

Tbx18 Regulates the Differentiation of Periductal Smooth Muscle Stroma and the Maintenance of Epithelial Integrity in the Prostate.
PloS one11(4)e0154413(2016)
Bolt CC,Negi S,Guimar?es-Camboa N,Zhang H,Troy JM,Lu X,Kispert A,Evans SM,Stubbs L

Ethanol Activation of PKA Mediates Single-Minded 2 Expression in Neuronal Cells.
Molecular neurobiology52(3)1234-1244(2015 Dec)
Wang X,Yang Z,Sun Y,Zhou H,Chu G,Zhang J,Meng X

HSA21 Single-Minded 2 (Sim2) Binding Sites Co-Localize with Super-Enhancers and Pioneer Transcription Factors in Pluripotent Mouse ES Cells.
PloS one10(5)e0126475(2015)
Letourneau A,Cobellis G,Fort A,Santoni F,Garieri M,Falconnet E,Ribaux P,Vannier A,Guipponi M,Carninci P,Borel C,Antonarakis SE

SIM2 maintains innate host defense of the small intestine.
American journal of physiology. Gastrointestinal and liver physiology307(11)G1044-56(2014 Dec)
Chen KJ,Lizaso A,Lee YH

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Sim2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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