TPCN1 cDNA ORF clone, Homo sapiens(human)

The following TPCN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TPCN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu02615 NM_017901.6
Latest version!
Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu02615 XM_011538492.2
Latest version!
Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu68190 XM_011538489.1
Latest version!
Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu46641 NM_001351347.1
Latest version!
Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OHu46641 NM_001351346.2
Latest version!
Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OHu46641 NM_001301214.1
Latest version!
Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OHu46641 XM_011538493.2
Latest version!
Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OHu31800 NM_001143819.3
Latest version!
Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu31800 XM_011538490.2
Latest version!
Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu83894 XM_017019480.2
Latest version!
Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu83895 XM_017019482.2
Latest version!
Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OHu83894 XM_017019480.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu02615 NM_017901.5 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu83895 XM_017019482.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu31800 NM_001143819.2 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu02615 XM_011538492.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu46641 NM_001351346.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu02615
    Clone ID Related Accession (Same CDS sequence) XM_011538492.1 , NM_017901.6 , NM_017901.5 , XM_011538492.2
    Accession Version NM_017901.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-01
    Organism Homo sapiens(human)
    Product two pore calcium channel protein 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK093490.1 and BC150203.1. On Jun 2, 2019 this sequence version replaced NM_017901.5. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150203.1, AB032995.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTGGATGA CGACGTGCCG CTCATCCTGA CCTTGGATGA GGGTGGCAGT 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CAAGAAGAGC TACCTAGCAA AAATGGCGGC
    AGCTATGCCA TCCACGACTC CCAGGCCCCC AGTCTCAGCT CTGGGGGTGA GAGTTCCCCC
    TCCAGCCCCG CACACAACTG GGAGATGAAT TACCAAGAGG CAGCAATCTA CCTCCAGGAA
    GGCGAGAACA ACGACAAGTT CTTCACCCAC CCCAAGGATG CCAAGGCGCT GGCGGCCTAC
    CTCTTTGCAC ACAATCACCT CTTCTACCTG ATGGAGCTGG CCACGGCCCT GCTGCTGCTG
    CTGCTCTCCC TGTGCGAGGC CCCCGCCGTC CCCGCACTCC GGCTTGGCAT CTATGTCCAC
    GCCACCCTGG AGCTGTTTGC CCTGATGGTG GTAGTGTTTG AACTCTGCAT GAAGTTACGC
    TGGCTGGGCC TCCACACCTT CATCCGGCAC AAGCGGACCA TGGTCAAGAC CTCGGTGCTG
    GTGGTGCAGT TTGTCGAGGC CATCGTGGTG TTGGTACGGC AGATGTCCCA TGTGCGGGTG
    ACCCGAGCAC TGCGCTGCAT TTTCCTGGTG GACTGTCGGT ATTGCGGTGG CGTCCGGCGC
    AACCTGCGGC AGATCTTCCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTGCTGCTG
    TTCTTCATGA TCATCTTTGC CATCCTCGGT TTCTACTTGT TCTCCCCTAA CCCTTCAGAC
    CCCTACTTCA GCACCCTGGA GAACAGCATC GTCAGTCTGT TTGTCCTTCT GACCACAGCC
    AATTTCCCAG ATGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC
    ATCGTGTACC TCTCCATCGA GCTGTATTTC ATCATGAACC TGCTTCTGGC TGTGGTGTTC
    GACACCTTCA ATGACATTGA GAAACGCAAG TTCAAGTCTT TGCTACTGCA CAAGCGAACC
    GCTATCCAGC ATGCCTACCG CCTGCTCATC AGCCAGAGGA GGCCTGCCGG CATCTCCTAC
    AGGCAGTTTG AAGGCCTCAT GCGCTTCTAC AAGCCCCGGA TGAGTGCCAG GGAGCGCTAT
    CTTACCTTCA AGGCCCTGAA TCAGAACAAC ACACCCCTGC TCAGCCTAAA GGACTTTTAC
    GATATCTACG AAGTTGCTGC TTTGAAGTGG AAGGCCAAGA AAAACAGAGA GCACTGGTTT
    GATGAGCTTC CCAGGACGGC GCTCCTCATC TTCAAAGGTA TTAATATCCT TGTGAAGTCC
    AAGGCCTTCC AGTATTTCAT GTACTTGGTG GTGGCAGTCA ACGGGGTCTG GATCCTCGTG
    GAGACATTTA TGCTGAAAGG TGGGAACTTC TTCTCCAAGC ACGTGCCCTG GAGTTACCTC
    GTCTTTCTAA CTATCTATGG GGTGGAGCTG TTCCTGAAGG TTGCCGGCCT GGGCCCTGTG
    GAGTACTTGT CTTCCGGATG GAACTTGTTT GACTTCTCCG TGACAGTGTT CGCCTTCCTG
    GGACTGCTGG CGCTGGCCCT CAACATGGAG CCCTTCTATT TCATCGTGGT CCTGCGCCCC
    CTCCAGCTGC TGAGGTTGTT TAAGTTGAAG GAGCGCTACC GCAACGTGCT GGACACCATG
    TTCGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTCACCC TGCTCATCTT TTACTACTCC
    TTCGCCATCG TGGGCATGGA GTTCTTCTGC GGGATCGTCT TCCCCAACTG CTGCAACACG
    AGTACAGTGG CAGATGCCTA CCGCTGGCGC AACCACACCG TGGGCAACAG GACCGTGGTG
    GAGGAAGGCT ACTATTATCT CAATAATTTT GACAACATCC TCAACAGCTT TGTGACCCTG
    TTTGAGCTCA CAGTTGTCAA CAACTGGTAC ATCATCATGG AAGGCGTCAC CTCTCAGACC
    TCCCACTGGA GCCGCCTCTA CTTCATGACC TTTTACATTG TGACCATGGT GGTGATGACG
    ATCATTGTCG CCTTTATCCT CGAGGCCTTC GTCTTCCGAA TGAACTACAG CCGCAAGAAC
    CAGGACTCGG AAGTTGATGG TGGCATCACC CTTGAGAAGG AAATCTCCAA AGAAGAGCTG
    GTTGCCGTCC TGGAGCTCTA CCGGGAGGCA CGGGGGGCCT CCTCGGATGT CACCAGGCTG
    CTGGAGACCC TCTCCCAGAT GGAGAGATAC CAGCAACATT CCATGGTGTT TCTGGGACGG
    CGATCAAGGA CCAAGAGCGA CCTGAGCCTG AAGATGTACC AGGAGGAGAT CCAGGAGTGG
    TATGAGGAGC ATGCCAGGGA GCAAGAGCAG CAGCGACAAC TCAGCAGCAG TGCAGCCCCC
    GCCGCCCAGC AGCCCCCAGG CAGCCGCCAG CGCTCCCAGA CCGTTACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060371.2
    CDS289..2739
    Translation

    Target ORF information:

    RefSeq Version NM_017901.6
    Organism Homo sapiens(human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_017901.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTGGATGA CGACGTGCCG CTCATCCTGA CCTTGGATGA GGGTGGCAGT 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CAAGAAGAGC TACCTAGCAA AAATGGCGGC
    AGCTATGCCA TCCACGACTC CCAGGCCCCC AGTCTCAGCT CTGGGGGTGA GAGTTCCCCC
    TCCAGCCCCG CACACAACTG GGAGATGAAT TACCAAGAGG CAGCAATCTA CCTCCAGGAA
    GGCGAGAACA ACGACAAGTT CTTCACCCAC CCCAAGGATG CCAAGGCGCT GGCGGCCTAC
    CTCTTTGCAC ACAATCACCT CTTCTACCTG ATGGAGCTGG CCACGGCCCT GCTGCTGCTG
    CTGCTCTCCC TGTGCGAGGC CCCCGCCGTC CCCGCACTCC GGCTTGGCAT CTATGTCCAC
    GCCACCCTGG AGCTGTTTGC CCTGATGGTG GTAGTGTTTG AACTCTGCAT GAAGTTACGC
    TGGCTGGGCC TCCACACCTT CATCCGGCAC AAGCGGACCA TGGTCAAGAC CTCGGTGCTG
    GTGGTGCAGT TTGTCGAGGC CATCGTGGTG TTGGTACGGC AGATGTCCCA TGTGCGGGTG
    ACCCGAGCAC TGCGCTGCAT TTTCCTGGTG GACTGTCGGT ATTGCGGTGG CGTCCGGCGC
    AACCTGCGGC AGATCTTCCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTGCTGCTG
    TTCTTCATGA TCATCTTTGC CATCCTCGGT TTCTACTTGT TCTCCCCTAA CCCTTCAGAC
    CCCTACTTCA GCACCCTGGA GAACAGCATC GTCAGTCTGT TTGTCCTTCT GACCACAGCC
    AATTTCCCAG ATGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC
    ATCGTGTACC TCTCCATCGA GCTGTATTTC ATCATGAACC TGCTTCTGGC TGTGGTGTTC
    GACACCTTCA ATGACATTGA GAAACGCAAG TTCAAGTCTT TGCTACTGCA CAAGCGAACC
    GCTATCCAGC ATGCCTACCG CCTGCTCATC AGCCAGAGGA GGCCTGCCGG CATCTCCTAC
    AGGCAGTTTG AAGGCCTCAT GCGCTTCTAC AAGCCCCGGA TGAGTGCCAG GGAGCGCTAT
    CTTACCTTCA AGGCCCTGAA TCAGAACAAC ACACCCCTGC TCAGCCTAAA GGACTTTTAC
    GATATCTACG AAGTTGCTGC TTTGAAGTGG AAGGCCAAGA AAAACAGAGA GCACTGGTTT
    GATGAGCTTC CCAGGACGGC GCTCCTCATC TTCAAAGGTA TTAATATCCT TGTGAAGTCC
    AAGGCCTTCC AGTATTTCAT GTACTTGGTG GTGGCAGTCA ACGGGGTCTG GATCCTCGTG
    GAGACATTTA TGCTGAAAGG TGGGAACTTC TTCTCCAAGC ACGTGCCCTG GAGTTACCTC
    GTCTTTCTAA CTATCTATGG GGTGGAGCTG TTCCTGAAGG TTGCCGGCCT GGGCCCTGTG
    GAGTACTTGT CTTCCGGATG GAACTTGTTT GACTTCTCCG TGACAGTGTT CGCCTTCCTG
    GGACTGCTGG CGCTGGCCCT CAACATGGAG CCCTTCTATT TCATCGTGGT CCTGCGCCCC
    CTCCAGCTGC TGAGGTTGTT TAAGTTGAAG GAGCGCTACC GCAACGTGCT GGACACCATG
    TTCGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTCACCC TGCTCATCTT TTACTACTCC
    TTCGCCATCG TGGGCATGGA GTTCTTCTGC GGGATCGTCT TCCCCAACTG CTGCAACACG
    AGTACAGTGG CAGATGCCTA CCGCTGGCGC AACCACACCG TGGGCAACAG GACCGTGGTG
    GAGGAAGGCT ACTATTATCT CAATAATTTT GACAACATCC TCAACAGCTT TGTGACCCTG
    TTTGAGCTCA CAGTTGTCAA CAACTGGTAC ATCATCATGG AAGGCGTCAC CTCTCAGACC
    TCCCACTGGA GCCGCCTCTA CTTCATGACC TTTTACATTG TGACCATGGT GGTGATGACG
    ATCATTGTCG CCTTTATCCT CGAGGCCTTC GTCTTCCGAA TGAACTACAG CCGCAAGAAC
    CAGGACTCGG AAGTTGATGG TGGCATCACC CTTGAGAAGG AAATCTCCAA AGAAGAGCTG
    GTTGCCGTCC TGGAGCTCTA CCGGGAGGCA CGGGGGGCCT CCTCGGATGT CACCAGGCTG
    CTGGAGACCC TCTCCCAGAT GGAGAGATAC CAGCAACATT CCATGGTGTT TCTGGGACGG
    CGATCAAGGA CCAAGAGCGA CCTGAGCCTG AAGATGTACC AGGAGGAGAT CCAGGAGTGG
    TATGAGGAGC ATGCCAGGGA GCAAGAGCAG CAGCGACAAC TCAGCAGCAG TGCAGCCCCC
    GCCGCCCAGC AGCCCCCAGG CAGCCGCCAG CGCTCCCAGA CCGTTACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02615
    Clone ID Related Accession (Same CDS sequence) XM_011538492.1 , NM_017901.6 , NM_017901.5 , XM_011538492.2
    Accession Version NM_017901.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product two pore calcium channel protein 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK093490.1 and BC150203.1. On Jul 25, 2014 this sequence version replaced NM_017901.4. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150203.1, AK093490.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTGGATGA CGACGTGCCG CTCATCCTGA CCTTGGATGA GGGTGGCAGT 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CAAGAAGAGC TACCTAGCAA AAATGGCGGC
    AGCTATGCCA TCCACGACTC CCAGGCCCCC AGTCTCAGCT CTGGGGGTGA GAGTTCCCCC
    TCCAGCCCCG CACACAACTG GGAGATGAAT TACCAAGAGG CAGCAATCTA CCTCCAGGAA
    GGCGAGAACA ACGACAAGTT CTTCACCCAC CCCAAGGATG CCAAGGCGCT GGCGGCCTAC
    CTCTTTGCAC ACAATCACCT CTTCTACCTG ATGGAGCTGG CCACGGCCCT GCTGCTGCTG
    CTGCTCTCCC TGTGCGAGGC CCCCGCCGTC CCCGCACTCC GGCTTGGCAT CTATGTCCAC
    GCCACCCTGG AGCTGTTTGC CCTGATGGTG GTAGTGTTTG AACTCTGCAT GAAGTTACGC
    TGGCTGGGCC TCCACACCTT CATCCGGCAC AAGCGGACCA TGGTCAAGAC CTCGGTGCTG
    GTGGTGCAGT TTGTCGAGGC CATCGTGGTG TTGGTACGGC AGATGTCCCA TGTGCGGGTG
    ACCCGAGCAC TGCGCTGCAT TTTCCTGGTG GACTGTCGGT ATTGCGGTGG CGTCCGGCGC
    AACCTGCGGC AGATCTTCCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTGCTGCTG
    TTCTTCATGA TCATCTTTGC CATCCTCGGT TTCTACTTGT TCTCCCCTAA CCCTTCAGAC
    CCCTACTTCA GCACCCTGGA GAACAGCATC GTCAGTCTGT TTGTCCTTCT GACCACAGCC
    AATTTCCCAG ATGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC
    ATCGTGTACC TCTCCATCGA GCTGTATTTC ATCATGAACC TGCTTCTGGC TGTGGTGTTC
    GACACCTTCA ATGACATTGA GAAACGCAAG TTCAAGTCTT TGCTACTGCA CAAGCGAACC
    GCTATCCAGC ATGCCTACCG CCTGCTCATC AGCCAGAGGA GGCCTGCCGG CATCTCCTAC
    AGGCAGTTTG AAGGCCTCAT GCGCTTCTAC AAGCCCCGGA TGAGTGCCAG GGAGCGCTAT
    CTTACCTTCA AGGCCCTGAA TCAGAACAAC ACACCCCTGC TCAGCCTAAA GGACTTTTAC
    GATATCTACG AAGTTGCTGC TTTGAAGTGG AAGGCCAAGA AAAACAGAGA GCACTGGTTT
    GATGAGCTTC CCAGGACGGC GCTCCTCATC TTCAAAGGTA TTAATATCCT TGTGAAGTCC
    AAGGCCTTCC AGTATTTCAT GTACTTGGTG GTGGCAGTCA ACGGGGTCTG GATCCTCGTG
    GAGACATTTA TGCTGAAAGG TGGGAACTTC TTCTCCAAGC ACGTGCCCTG GAGTTACCTC
    GTCTTTCTAA CTATCTATGG GGTGGAGCTG TTCCTGAAGG TTGCCGGCCT GGGCCCTGTG
    GAGTACTTGT CTTCCGGATG GAACTTGTTT GACTTCTCCG TGACAGTGTT CGCCTTCCTG
    GGACTGCTGG CGCTGGCCCT CAACATGGAG CCCTTCTATT TCATCGTGGT CCTGCGCCCC
    CTCCAGCTGC TGAGGTTGTT TAAGTTGAAG GAGCGCTACC GCAACGTGCT GGACACCATG
    TTCGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTCACCC TGCTCATCTT TTACTACTCC
    TTCGCCATCG TGGGCATGGA GTTCTTCTGC GGGATCGTCT TCCCCAACTG CTGCAACACG
    AGTACAGTGG CAGATGCCTA CCGCTGGCGC AACCACACCG TGGGCAACAG GACCGTGGTG
    GAGGAAGGCT ACTATTATCT CAATAATTTT GACAACATCC TCAACAGCTT TGTGACCCTG
    TTTGAGCTCA CAGTTGTCAA CAACTGGTAC ATCATCATGG AAGGCGTCAC CTCTCAGACC
    TCCCACTGGA GCCGCCTCTA CTTCATGACC TTTTACATTG TGACCATGGT GGTGATGACG
    ATCATTGTCG CCTTTATCCT CGAGGCCTTC GTCTTCCGAA TGAACTACAG CCGCAAGAAC
    CAGGACTCGG AAGTTGATGG TGGCATCACC CTTGAGAAGG AAATCTCCAA AGAAGAGCTG
    GTTGCCGTCC TGGAGCTCTA CCGGGAGGCA CGGGGGGCCT CCTCGGATGT CACCAGGCTG
    CTGGAGACCC TCTCCCAGAT GGAGAGATAC CAGCAACATT CCATGGTGTT TCTGGGACGG
    CGATCAAGGA CCAAGAGCGA CCTGAGCCTG AAGATGTACC AGGAGGAGAT CCAGGAGTGG
    TATGAGGAGC ATGCCAGGGA GCAAGAGCAG CAGCGACAAC TCAGCAGCAG TGCAGCCCCC
    GCCGCCCAGC AGCCCCCAGG CAGCCGCCAG CGCTCCCAGA CCGTTACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060371.2
    CDS315..2765
    Translation

    Target ORF information:

    RefSeq Version NM_017901.5
    Organism Homo sapiens(human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_017901.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTGGATGA CGACGTGCCG CTCATCCTGA CCTTGGATGA GGGTGGCAGT 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CAAGAAGAGC TACCTAGCAA AAATGGCGGC
    AGCTATGCCA TCCACGACTC CCAGGCCCCC AGTCTCAGCT CTGGGGGTGA GAGTTCCCCC
    TCCAGCCCCG CACACAACTG GGAGATGAAT TACCAAGAGG CAGCAATCTA CCTCCAGGAA
    GGCGAGAACA ACGACAAGTT CTTCACCCAC CCCAAGGATG CCAAGGCGCT GGCGGCCTAC
    CTCTTTGCAC ACAATCACCT CTTCTACCTG ATGGAGCTGG CCACGGCCCT GCTGCTGCTG
    CTGCTCTCCC TGTGCGAGGC CCCCGCCGTC CCCGCACTCC GGCTTGGCAT CTATGTCCAC
    GCCACCCTGG AGCTGTTTGC CCTGATGGTG GTAGTGTTTG AACTCTGCAT GAAGTTACGC
    TGGCTGGGCC TCCACACCTT CATCCGGCAC AAGCGGACCA TGGTCAAGAC CTCGGTGCTG
    GTGGTGCAGT TTGTCGAGGC CATCGTGGTG TTGGTACGGC AGATGTCCCA TGTGCGGGTG
    ACCCGAGCAC TGCGCTGCAT TTTCCTGGTG GACTGTCGGT ATTGCGGTGG CGTCCGGCGC
    AACCTGCGGC AGATCTTCCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTGCTGCTG
    TTCTTCATGA TCATCTTTGC CATCCTCGGT TTCTACTTGT TCTCCCCTAA CCCTTCAGAC
    CCCTACTTCA GCACCCTGGA GAACAGCATC GTCAGTCTGT TTGTCCTTCT GACCACAGCC
    AATTTCCCAG ATGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC
    ATCGTGTACC TCTCCATCGA GCTGTATTTC ATCATGAACC TGCTTCTGGC TGTGGTGTTC
    GACACCTTCA ATGACATTGA GAAACGCAAG TTCAAGTCTT TGCTACTGCA CAAGCGAACC
    GCTATCCAGC ATGCCTACCG CCTGCTCATC AGCCAGAGGA GGCCTGCCGG CATCTCCTAC
    AGGCAGTTTG AAGGCCTCAT GCGCTTCTAC AAGCCCCGGA TGAGTGCCAG GGAGCGCTAT
    CTTACCTTCA AGGCCCTGAA TCAGAACAAC ACACCCCTGC TCAGCCTAAA GGACTTTTAC
    GATATCTACG AAGTTGCTGC TTTGAAGTGG AAGGCCAAGA AAAACAGAGA GCACTGGTTT
    GATGAGCTTC CCAGGACGGC GCTCCTCATC TTCAAAGGTA TTAATATCCT TGTGAAGTCC
    AAGGCCTTCC AGTATTTCAT GTACTTGGTG GTGGCAGTCA ACGGGGTCTG GATCCTCGTG
    GAGACATTTA TGCTGAAAGG TGGGAACTTC TTCTCCAAGC ACGTGCCCTG GAGTTACCTC
    GTCTTTCTAA CTATCTATGG GGTGGAGCTG TTCCTGAAGG TTGCCGGCCT GGGCCCTGTG
    GAGTACTTGT CTTCCGGATG GAACTTGTTT GACTTCTCCG TGACAGTGTT CGCCTTCCTG
    GGACTGCTGG CGCTGGCCCT CAACATGGAG CCCTTCTATT TCATCGTGGT CCTGCGCCCC
    CTCCAGCTGC TGAGGTTGTT TAAGTTGAAG GAGCGCTACC GCAACGTGCT GGACACCATG
    TTCGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTCACCC TGCTCATCTT TTACTACTCC
    TTCGCCATCG TGGGCATGGA GTTCTTCTGC GGGATCGTCT TCCCCAACTG CTGCAACACG
    AGTACAGTGG CAGATGCCTA CCGCTGGCGC AACCACACCG TGGGCAACAG GACCGTGGTG
    GAGGAAGGCT ACTATTATCT CAATAATTTT GACAACATCC TCAACAGCTT TGTGACCCTG
    TTTGAGCTCA CAGTTGTCAA CAACTGGTAC ATCATCATGG AAGGCGTCAC CTCTCAGACC
    TCCCACTGGA GCCGCCTCTA CTTCATGACC TTTTACATTG TGACCATGGT GGTGATGACG
    ATCATTGTCG CCTTTATCCT CGAGGCCTTC GTCTTCCGAA TGAACTACAG CCGCAAGAAC
    CAGGACTCGG AAGTTGATGG TGGCATCACC CTTGAGAAGG AAATCTCCAA AGAAGAGCTG
    GTTGCCGTCC TGGAGCTCTA CCGGGAGGCA CGGGGGGCCT CCTCGGATGT CACCAGGCTG
    CTGGAGACCC TCTCCCAGAT GGAGAGATAC CAGCAACATT CCATGGTGTT TCTGGGACGG
    CGATCAAGGA CCAAGAGCGA CCTGAGCCTG AAGATGTACC AGGAGGAGAT CCAGGAGTGG
    TATGAGGAGC ATGCCAGGGA GCAAGAGCAG CAGCGACAAC TCAGCAGCAG TGCAGCCCCC
    GCCGCCCAGC AGCCCCCAGG CAGCCGCCAG CGCTCCCAGA CCGTTACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02615
    Clone ID Related Accession (Same CDS sequence) XM_011538492.1 , NM_017901.6 , NM_017901.5 , XM_011538492.2
    Accession Version XM_011538492.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product two pore calcium channel protein 1 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTGGATGA CGACGTGCCG CTCATCCTGA CCTTGGATGA GGGTGGCAGT 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CAAGAAGAGC TACCTAGCAA AAATGGCGGC
    AGCTATGCCA TCCACGACTC CCAGGCCCCC AGTCTCAGCT CTGGGGGTGA GAGTTCCCCC
    TCCAGCCCCG CACACAACTG GGAGATGAAT TACCAAGAGG CAGCAATCTA CCTCCAGGAA
    GGCGAGAACA ACGACAAGTT CTTCACCCAC CCCAAGGATG CCAAGGCGCT GGCGGCCTAC
    CTCTTTGCAC ACAATCACCT CTTCTACCTG ATGGAGCTGG CCACGGCCCT GCTGCTGCTG
    CTGCTCTCCC TGTGCGAGGC CCCCGCCGTC CCCGCACTCC GGCTTGGCAT CTATGTCCAC
    GCCACCCTGG AGCTGTTTGC CCTGATGGTG GTAGTGTTTG AACTCTGCAT GAAGTTACGC
    TGGCTGGGCC TCCACACCTT CATCCGGCAC AAGCGGACCA TGGTCAAGAC CTCGGTGCTG
    GTGGTGCAGT TTGTCGAGGC CATCGTGGTG TTGGTACGGC AGATGTCCCA TGTGCGGGTG
    ACCCGAGCAC TGCGCTGCAT TTTCCTGGTG GACTGTCGGT ATTGCGGTGG CGTCCGGCGC
    AACCTGCGGC AGATCTTCCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTGCTGCTG
    TTCTTCATGA TCATCTTTGC CATCCTCGGT TTCTACTTGT TCTCCCCTAA CCCTTCAGAC
    CCCTACTTCA GCACCCTGGA GAACAGCATC GTCAGTCTGT TTGTCCTTCT GACCACAGCC
    AATTTCCCAG ATGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC
    ATCGTGTACC TCTCCATCGA GCTGTATTTC ATCATGAACC TGCTTCTGGC TGTGGTGTTC
    GACACCTTCA ATGACATTGA GAAACGCAAG TTCAAGTCTT TGCTACTGCA CAAGCGAACC
    GCTATCCAGC ATGCCTACCG CCTGCTCATC AGCCAGAGGA GGCCTGCCGG CATCTCCTAC
    AGGCAGTTTG AAGGCCTCAT GCGCTTCTAC AAGCCCCGGA TGAGTGCCAG GGAGCGCTAT
    CTTACCTTCA AGGCCCTGAA TCAGAACAAC ACACCCCTGC TCAGCCTAAA GGACTTTTAC
    GATATCTACG AAGTTGCTGC TTTGAAGTGG AAGGCCAAGA AAAACAGAGA GCACTGGTTT
    GATGAGCTTC CCAGGACGGC GCTCCTCATC TTCAAAGGTA TTAATATCCT TGTGAAGTCC
    AAGGCCTTCC AGTATTTCAT GTACTTGGTG GTGGCAGTCA ACGGGGTCTG GATCCTCGTG
    GAGACATTTA TGCTGAAAGG TGGGAACTTC TTCTCCAAGC ACGTGCCCTG GAGTTACCTC
    GTCTTTCTAA CTATCTATGG GGTGGAGCTG TTCCTGAAGG TTGCCGGCCT GGGCCCTGTG
    GAGTACTTGT CTTCCGGATG GAACTTGTTT GACTTCTCCG TGACAGTGTT CGCCTTCCTG
    GGACTGCTGG CGCTGGCCCT CAACATGGAG CCCTTCTATT TCATCGTGGT CCTGCGCCCC
    CTCCAGCTGC TGAGGTTGTT TAAGTTGAAG GAGCGCTACC GCAACGTGCT GGACACCATG
    TTCGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTCACCC TGCTCATCTT TTACTACTCC
    TTCGCCATCG TGGGCATGGA GTTCTTCTGC GGGATCGTCT TCCCCAACTG CTGCAACACG
    AGTACAGTGG CAGATGCCTA CCGCTGGCGC AACCACACCG TGGGCAACAG GACCGTGGTG
    GAGGAAGGCT ACTATTATCT CAATAATTTT GACAACATCC TCAACAGCTT TGTGACCCTG
    TTTGAGCTCA CAGTTGTCAA CAACTGGTAC ATCATCATGG AAGGCGTCAC CTCTCAGACC
    TCCCACTGGA GCCGCCTCTA CTTCATGACC TTTTACATTG TGACCATGGT GGTGATGACG
    ATCATTGTCG CCTTTATCCT CGAGGCCTTC GTCTTCCGAA TGAACTACAG CCGCAAGAAC
    CAGGACTCGG AAGTTGATGG TGGCATCACC CTTGAGAAGG AAATCTCCAA AGAAGAGCTG
    GTTGCCGTCC TGGAGCTCTA CCGGGAGGCA CGGGGGGCCT CCTCGGATGT CACCAGGCTG
    CTGGAGACCC TCTCCCAGAT GGAGAGATAC CAGCAACATT CCATGGTGTT TCTGGGACGG
    CGATCAAGGA CCAAGAGCGA CCTGAGCCTG AAGATGTACC AGGAGGAGAT CCAGGAGTGG
    TATGAGGAGC ATGCCAGGGA GCAAGAGCAG CAGCGACAAC TCAGCAGCAG TGCAGCCCCC
    GCCGCCCAGC AGCCCCCAGG CAGCCGCCAG CGCTCCCAGA CCGTTACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011536794.1
    Translation

    Target ORF information:

    RefSeq Version XM_011538492.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011538492.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTGGATGA CGACGTGCCG CTCATCCTGA CCTTGGATGA GGGTGGCAGT 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CAAGAAGAGC TACCTAGCAA AAATGGCGGC
    AGCTATGCCA TCCACGACTC CCAGGCCCCC AGTCTCAGCT CTGGGGGTGA GAGTTCCCCC
    TCCAGCCCCG CACACAACTG GGAGATGAAT TACCAAGAGG CAGCAATCTA CCTCCAGGAA
    GGCGAGAACA ACGACAAGTT CTTCACCCAC CCCAAGGATG CCAAGGCGCT GGCGGCCTAC
    CTCTTTGCAC ACAATCACCT CTTCTACCTG ATGGAGCTGG CCACGGCCCT GCTGCTGCTG
    CTGCTCTCCC TGTGCGAGGC CCCCGCCGTC CCCGCACTCC GGCTTGGCAT CTATGTCCAC
    GCCACCCTGG AGCTGTTTGC CCTGATGGTG GTAGTGTTTG AACTCTGCAT GAAGTTACGC
    TGGCTGGGCC TCCACACCTT CATCCGGCAC AAGCGGACCA TGGTCAAGAC CTCGGTGCTG
    GTGGTGCAGT TTGTCGAGGC CATCGTGGTG TTGGTACGGC AGATGTCCCA TGTGCGGGTG
    ACCCGAGCAC TGCGCTGCAT TTTCCTGGTG GACTGTCGGT ATTGCGGTGG CGTCCGGCGC
    AACCTGCGGC AGATCTTCCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTGCTGCTG
    TTCTTCATGA TCATCTTTGC CATCCTCGGT TTCTACTTGT TCTCCCCTAA CCCTTCAGAC
    CCCTACTTCA GCACCCTGGA GAACAGCATC GTCAGTCTGT TTGTCCTTCT GACCACAGCC
    AATTTCCCAG ATGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC
    ATCGTGTACC TCTCCATCGA GCTGTATTTC ATCATGAACC TGCTTCTGGC TGTGGTGTTC
    GACACCTTCA ATGACATTGA GAAACGCAAG TTCAAGTCTT TGCTACTGCA CAAGCGAACC
    GCTATCCAGC ATGCCTACCG CCTGCTCATC AGCCAGAGGA GGCCTGCCGG CATCTCCTAC
    AGGCAGTTTG AAGGCCTCAT GCGCTTCTAC AAGCCCCGGA TGAGTGCCAG GGAGCGCTAT
    CTTACCTTCA AGGCCCTGAA TCAGAACAAC ACACCCCTGC TCAGCCTAAA GGACTTTTAC
    GATATCTACG AAGTTGCTGC TTTGAAGTGG AAGGCCAAGA AAAACAGAGA GCACTGGTTT
    GATGAGCTTC CCAGGACGGC GCTCCTCATC TTCAAAGGTA TTAATATCCT TGTGAAGTCC
    AAGGCCTTCC AGTATTTCAT GTACTTGGTG GTGGCAGTCA ACGGGGTCTG GATCCTCGTG
    GAGACATTTA TGCTGAAAGG TGGGAACTTC TTCTCCAAGC ACGTGCCCTG GAGTTACCTC
    GTCTTTCTAA CTATCTATGG GGTGGAGCTG TTCCTGAAGG TTGCCGGCCT GGGCCCTGTG
    GAGTACTTGT CTTCCGGATG GAACTTGTTT GACTTCTCCG TGACAGTGTT CGCCTTCCTG
    GGACTGCTGG CGCTGGCCCT CAACATGGAG CCCTTCTATT TCATCGTGGT CCTGCGCCCC
    CTCCAGCTGC TGAGGTTGTT TAAGTTGAAG GAGCGCTACC GCAACGTGCT GGACACCATG
    TTCGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTCACCC TGCTCATCTT TTACTACTCC
    TTCGCCATCG TGGGCATGGA GTTCTTCTGC GGGATCGTCT TCCCCAACTG CTGCAACACG
    AGTACAGTGG CAGATGCCTA CCGCTGGCGC AACCACACCG TGGGCAACAG GACCGTGGTG
    GAGGAAGGCT ACTATTATCT CAATAATTTT GACAACATCC TCAACAGCTT TGTGACCCTG
    TTTGAGCTCA CAGTTGTCAA CAACTGGTAC ATCATCATGG AAGGCGTCAC CTCTCAGACC
    TCCCACTGGA GCCGCCTCTA CTTCATGACC TTTTACATTG TGACCATGGT GGTGATGACG
    ATCATTGTCG CCTTTATCCT CGAGGCCTTC GTCTTCCGAA TGAACTACAG CCGCAAGAAC
    CAGGACTCGG AAGTTGATGG TGGCATCACC CTTGAGAAGG AAATCTCCAA AGAAGAGCTG
    GTTGCCGTCC TGGAGCTCTA CCGGGAGGCA CGGGGGGCCT CCTCGGATGT CACCAGGCTG
    CTGGAGACCC TCTCCCAGAT GGAGAGATAC CAGCAACATT CCATGGTGTT TCTGGGACGG
    CGATCAAGGA CCAAGAGCGA CCTGAGCCTG AAGATGTACC AGGAGGAGAT CCAGGAGTGG
    TATGAGGAGC ATGCCAGGGA GCAAGAGCAG CAGCGACAAC TCAGCAGCAG TGCAGCCCCC
    GCCGCCCAGC AGCCCCCAGG CAGCCGCCAG CGCTCCCAGA CCGTTACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02615
    Clone ID Related Accession (Same CDS sequence) XM_011538492.1 , NM_017901.6 , NM_017901.5 , XM_011538492.2
    Accession Version XM_011538492.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product two pore calcium channel protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTGGATGA CGACGTGCCG CTCATCCTGA CCTTGGATGA GGGTGGCAGT 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CAAGAAGAGC TACCTAGCAA AAATGGCGGC
    AGCTATGCCA TCCACGACTC CCAGGCCCCC AGTCTCAGCT CTGGGGGTGA GAGTTCCCCC
    TCCAGCCCCG CACACAACTG GGAGATGAAT TACCAAGAGG CAGCAATCTA CCTCCAGGAA
    GGCGAGAACA ACGACAAGTT CTTCACCCAC CCCAAGGATG CCAAGGCGCT GGCGGCCTAC
    CTCTTTGCAC ACAATCACCT CTTCTACCTG ATGGAGCTGG CCACGGCCCT GCTGCTGCTG
    CTGCTCTCCC TGTGCGAGGC CCCCGCCGTC CCCGCACTCC GGCTTGGCAT CTATGTCCAC
    GCCACCCTGG AGCTGTTTGC CCTGATGGTG GTAGTGTTTG AACTCTGCAT GAAGTTACGC
    TGGCTGGGCC TCCACACCTT CATCCGGCAC AAGCGGACCA TGGTCAAGAC CTCGGTGCTG
    GTGGTGCAGT TTGTCGAGGC CATCGTGGTG TTGGTACGGC AGATGTCCCA TGTGCGGGTG
    ACCCGAGCAC TGCGCTGCAT TTTCCTGGTG GACTGTCGGT ATTGCGGTGG CGTCCGGCGC
    AACCTGCGGC AGATCTTCCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTGCTGCTG
    TTCTTCATGA TCATCTTTGC CATCCTCGGT TTCTACTTGT TCTCCCCTAA CCCTTCAGAC
    CCCTACTTCA GCACCCTGGA GAACAGCATC GTCAGTCTGT TTGTCCTTCT GACCACAGCC
    AATTTCCCAG ATGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC
    ATCGTGTACC TCTCCATCGA GCTGTATTTC ATCATGAACC TGCTTCTGGC TGTGGTGTTC
    GACACCTTCA ATGACATTGA GAAACGCAAG TTCAAGTCTT TGCTACTGCA CAAGCGAACC
    GCTATCCAGC ATGCCTACCG CCTGCTCATC AGCCAGAGGA GGCCTGCCGG CATCTCCTAC
    AGGCAGTTTG AAGGCCTCAT GCGCTTCTAC AAGCCCCGGA TGAGTGCCAG GGAGCGCTAT
    CTTACCTTCA AGGCCCTGAA TCAGAACAAC ACACCCCTGC TCAGCCTAAA GGACTTTTAC
    GATATCTACG AAGTTGCTGC TTTGAAGTGG AAGGCCAAGA AAAACAGAGA GCACTGGTTT
    GATGAGCTTC CCAGGACGGC GCTCCTCATC TTCAAAGGTA TTAATATCCT TGTGAAGTCC
    AAGGCCTTCC AGTATTTCAT GTACTTGGTG GTGGCAGTCA ACGGGGTCTG GATCCTCGTG
    GAGACATTTA TGCTGAAAGG TGGGAACTTC TTCTCCAAGC ACGTGCCCTG GAGTTACCTC
    GTCTTTCTAA CTATCTATGG GGTGGAGCTG TTCCTGAAGG TTGCCGGCCT GGGCCCTGTG
    GAGTACTTGT CTTCCGGATG GAACTTGTTT GACTTCTCCG TGACAGTGTT CGCCTTCCTG
    GGACTGCTGG CGCTGGCCCT CAACATGGAG CCCTTCTATT TCATCGTGGT CCTGCGCCCC
    CTCCAGCTGC TGAGGTTGTT TAAGTTGAAG GAGCGCTACC GCAACGTGCT GGACACCATG
    TTCGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTCACCC TGCTCATCTT TTACTACTCC
    TTCGCCATCG TGGGCATGGA GTTCTTCTGC GGGATCGTCT TCCCCAACTG CTGCAACACG
    AGTACAGTGG CAGATGCCTA CCGCTGGCGC AACCACACCG TGGGCAACAG GACCGTGGTG
    GAGGAAGGCT ACTATTATCT CAATAATTTT GACAACATCC TCAACAGCTT TGTGACCCTG
    TTTGAGCTCA CAGTTGTCAA CAACTGGTAC ATCATCATGG AAGGCGTCAC CTCTCAGACC
    TCCCACTGGA GCCGCCTCTA CTTCATGACC TTTTACATTG TGACCATGGT GGTGATGACG
    ATCATTGTCG CCTTTATCCT CGAGGCCTTC GTCTTCCGAA TGAACTACAG CCGCAAGAAC
    CAGGACTCGG AAGTTGATGG TGGCATCACC CTTGAGAAGG AAATCTCCAA AGAAGAGCTG
    GTTGCCGTCC TGGAGCTCTA CCGGGAGGCA CGGGGGGCCT CCTCGGATGT CACCAGGCTG
    CTGGAGACCC TCTCCCAGAT GGAGAGATAC CAGCAACATT CCATGGTGTT TCTGGGACGG
    CGATCAAGGA CCAAGAGCGA CCTGAGCCTG AAGATGTACC AGGAGGAGAT CCAGGAGTGG
    TATGAGGAGC ATGCCAGGGA GCAAGAGCAG CAGCGACAAC TCAGCAGCAG TGCAGCCCCC
    GCCGCCCAGC AGCCCCCAGG CAGCCGCCAG CGCTCCCAGA CCGTTACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011536794.1
    CDS221..2671
    Translation

    Target ORF information:

    RefSeq Version XM_011538492.2
    Organism Homo sapiens(human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011538492.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTGGATGA CGACGTGCCG CTCATCCTGA CCTTGGATGA GGGTGGCAGT 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CAAGAAGAGC TACCTAGCAA AAATGGCGGC
    AGCTATGCCA TCCACGACTC CCAGGCCCCC AGTCTCAGCT CTGGGGGTGA GAGTTCCCCC
    TCCAGCCCCG CACACAACTG GGAGATGAAT TACCAAGAGG CAGCAATCTA CCTCCAGGAA
    GGCGAGAACA ACGACAAGTT CTTCACCCAC CCCAAGGATG CCAAGGCGCT GGCGGCCTAC
    CTCTTTGCAC ACAATCACCT CTTCTACCTG ATGGAGCTGG CCACGGCCCT GCTGCTGCTG
    CTGCTCTCCC TGTGCGAGGC CCCCGCCGTC CCCGCACTCC GGCTTGGCAT CTATGTCCAC
    GCCACCCTGG AGCTGTTTGC CCTGATGGTG GTAGTGTTTG AACTCTGCAT GAAGTTACGC
    TGGCTGGGCC TCCACACCTT CATCCGGCAC AAGCGGACCA TGGTCAAGAC CTCGGTGCTG
    GTGGTGCAGT TTGTCGAGGC CATCGTGGTG TTGGTACGGC AGATGTCCCA TGTGCGGGTG
    ACCCGAGCAC TGCGCTGCAT TTTCCTGGTG GACTGTCGGT ATTGCGGTGG CGTCCGGCGC
    AACCTGCGGC AGATCTTCCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTGCTGCTG
    TTCTTCATGA TCATCTTTGC CATCCTCGGT TTCTACTTGT TCTCCCCTAA CCCTTCAGAC
    CCCTACTTCA GCACCCTGGA GAACAGCATC GTCAGTCTGT TTGTCCTTCT GACCACAGCC
    AATTTCCCAG ATGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC
    ATCGTGTACC TCTCCATCGA GCTGTATTTC ATCATGAACC TGCTTCTGGC TGTGGTGTTC
    GACACCTTCA ATGACATTGA GAAACGCAAG TTCAAGTCTT TGCTACTGCA CAAGCGAACC
    GCTATCCAGC ATGCCTACCG CCTGCTCATC AGCCAGAGGA GGCCTGCCGG CATCTCCTAC
    AGGCAGTTTG AAGGCCTCAT GCGCTTCTAC AAGCCCCGGA TGAGTGCCAG GGAGCGCTAT
    CTTACCTTCA AGGCCCTGAA TCAGAACAAC ACACCCCTGC TCAGCCTAAA GGACTTTTAC
    GATATCTACG AAGTTGCTGC TTTGAAGTGG AAGGCCAAGA AAAACAGAGA GCACTGGTTT
    GATGAGCTTC CCAGGACGGC GCTCCTCATC TTCAAAGGTA TTAATATCCT TGTGAAGTCC
    AAGGCCTTCC AGTATTTCAT GTACTTGGTG GTGGCAGTCA ACGGGGTCTG GATCCTCGTG
    GAGACATTTA TGCTGAAAGG TGGGAACTTC TTCTCCAAGC ACGTGCCCTG GAGTTACCTC
    GTCTTTCTAA CTATCTATGG GGTGGAGCTG TTCCTGAAGG TTGCCGGCCT GGGCCCTGTG
    GAGTACTTGT CTTCCGGATG GAACTTGTTT GACTTCTCCG TGACAGTGTT CGCCTTCCTG
    GGACTGCTGG CGCTGGCCCT CAACATGGAG CCCTTCTATT TCATCGTGGT CCTGCGCCCC
    CTCCAGCTGC TGAGGTTGTT TAAGTTGAAG GAGCGCTACC GCAACGTGCT GGACACCATG
    TTCGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTCACCC TGCTCATCTT TTACTACTCC
    TTCGCCATCG TGGGCATGGA GTTCTTCTGC GGGATCGTCT TCCCCAACTG CTGCAACACG
    AGTACAGTGG CAGATGCCTA CCGCTGGCGC AACCACACCG TGGGCAACAG GACCGTGGTG
    GAGGAAGGCT ACTATTATCT CAATAATTTT GACAACATCC TCAACAGCTT TGTGACCCTG
    TTTGAGCTCA CAGTTGTCAA CAACTGGTAC ATCATCATGG AAGGCGTCAC CTCTCAGACC
    TCCCACTGGA GCCGCCTCTA CTTCATGACC TTTTACATTG TGACCATGGT GGTGATGACG
    ATCATTGTCG CCTTTATCCT CGAGGCCTTC GTCTTCCGAA TGAACTACAG CCGCAAGAAC
    CAGGACTCGG AAGTTGATGG TGGCATCACC CTTGAGAAGG AAATCTCCAA AGAAGAGCTG
    GTTGCCGTCC TGGAGCTCTA CCGGGAGGCA CGGGGGGCCT CCTCGGATGT CACCAGGCTG
    CTGGAGACCC TCTCCCAGAT GGAGAGATAC CAGCAACATT CCATGGTGTT TCTGGGACGG
    CGATCAAGGA CCAAGAGCGA CCTGAGCCTG AAGATGTACC AGGAGGAGAT CCAGGAGTGG
    TATGAGGAGC ATGCCAGGGA GCAAGAGCAG CAGCGACAAC TCAGCAGCAG TGCAGCCCCC
    GCCGCCCAGC AGCCCCCAGG CAGCCGCCAG CGCTCCCAGA CCGTTACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68190
    Clone ID Related Accession (Same CDS sequence) XM_011538489.1
    Accession Version XM_011538489.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2709bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product two pore calcium channel protein 1 isoform X4
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAAAGAC TTGGGACTCC CTCTGGGTTG GACTCAACGA AGATGGAGTC TTGCTACATT 
    GCCCAGGCTG GTCTTGAACT GCTAGGCTCA AGCAGTTCTC CCACCTTGAC CTCCCAAAGC
    GCTGAGATTA CAGAAGATGC CTCTAATGGA GGAGTTTCTG AGCAGCACCC CTGGCCCAGT
    GGCTTTGAAA GGGAGCTCAA ACCAGAGACT ATTTCAAGCC CTGGATATCA TATCCTGAGG
    GCCACAGGAG AAGAGAACAT GGCTGTGAGT TTGGATGACG ACGTGCCGCT CATCCTGACC
    TTGGATGAGG GTGGCAGTGC CCCACTGGCT CCCTCCAACG GCCTGGGCCA AGAAGAGCTA
    CCTAGCAAAA ATGGCGGCAG CTATGCCATC CACGACTCCC AGGCCCCCAG TCTCAGCTCT
    GGGGGTGAGA GTTCCCCCTC CAGCCCCGCA CACAACTGGG AGATGAATTA CCAAGAGGCA
    GCAATCTACC TCCAGGAAGG CGAGAACAAC GACAAGTTCT TCACCCACCC CAAGGATGCC
    AAGGCGCTGG CGGCCTACCT CTTTGCACAC AATCACCTCT TCTACCTGAT GGAGCTGGCC
    ACGGCCCTGC TGCTGCTGCT GCTCTCCCTG TGCGAGGCCC CCGCCGTCCC CGCACTCCGG
    CTTGGCATCT ATGTCCACGC CACCCTGGAG CTGTTTGCCC TGATGGTGGT AGTGTTTGAA
    CTCTGCATGA AGTTACGCTG GCTGGGCCTC CACACCTTCA TCCGGCACAA GCGGACCATG
    GTCAAGACCT CGGTGCTGGT GGTGCAGTTT GTCGAGGCCA TCGTGGTGTT GGTACGGCAG
    ATGTCCCATG TGCGGGTGAC CCGAGCACTG CGCTGCATTT TCCTGGTGGA CTGTCGGTAT
    TGCGGTGGCG TCCGGCGCAA CCTGCGGCAG ATCTTCCAGT CCCTGCCGCC CTTCATGGAC
    ATCCTCCTGC TGCTGCTGTT CTTCATGATC ATCTTTGCCA TCCTCGGTTT CTACTTGTTC
    TCCCCTAACC CTTCAGACCC CTACTTCAGC ACCCTGGAGA ACAGCATCGT CAGTCTGTTT
    GTCCTTCTGA CCACAGCCAA TTTCCCAGAT GTGATGATGC CCTCCTACTC CCGGAACCCC
    TGGTCCTGCG TCTTCTTCAT CGTGTACCTC TCCATCGAGC TGTATTTCAT CATGAACCTG
    CTTCTGGCTG TGGTGTTCGA CACCTTCAAT GACATTGAGA AACGCAAGTT CAAGTCTTTG
    CTACTGCACA AGCGAACCGC TATCCAGCAT GCCTACCGCC TGCTCATCAG CCAGAGGAGG
    CCTGCCGGCA TCTCCTACAG GCAGTTTGAA GGCCTCATGC GCTTCTACAA GCCCCGGATG
    AGTGCCAGGG AGCGCTATCT TACCTTCAAG GCCCTGAATC AGAACAACAC ACCCCTGCTC
    AGCCTAAAGG ACTTTTACGA TATCTACGAA GTTGCTGCTT TGAAGTGGAA GGCCAAGAAA
    AACAGAGAGC ACTGGTTTGA TGAGCTTCCC AGGACGGCGC TCCTCATCTT CAAAGGTATT
    AATATCCTTG TGAAGTCCAA GGCCTTCCAG TATTTCATGT ACTTGGTGGT GGCAGTCAAC
    GGGGTCTGGA TCCTCGTGGA GACATTTATG CTGAAAGGTG GGAACTTCTT CTCCAAGCAC
    GTGCCCTGGA GTTACCTCGT CTTTCTAACT ATCTATGGGG TGGAGCTGTT CCTGAAGGTT
    GCCGGCCTGG GCCCTGTGGA GTACTTGTCT TCCGGATGGA ACTTGTTTGA CTTCTCCGTG
    ACAGTGTTCG CCTTCCTGGG ACTGCTGGCG CTGGCCCTCA ACATGGAGCC CTTCTATTTC
    ATCGTGGTCC TGCGCCCCCT CCAGCTGCTG AGGTTGTTTA AGTTGAAGGA GCGCTACCGC
    AACGTGCTGG ACACCATGTT CGAGCTGCTG CCCCGGATGG CCAGCCTGGG CCTCACCCTG
    CTCATCTTTT ACTACTCCTT CGCCATCGTG GGCATGGAGT TCTTCTGCGG GATCGTCTTC
    CCCAACTGCT GCAACACGAG TACAGTGGCA GATGCCTACC GCTGGCGCAA CCACACCGTG
    GGCAACAGGA CCGTGGTGGA GGAAGGCTAC TATTATCTCA ATAATTTTGA CAACATCCTC
    AACAGCTTTG TGACCCTGTT TGAGCTCACA GTTGTCAACA ACTGGTACAT CATCATGGAA
    GGCGTCACCT CTCAGACCTC CCACTGGAGC CGCCTCTACT TCATGACCTT TTACATTGTG
    ACCATGGTGG TGATGACGAT CATTGTCGCC TTTATCCTCG AGGCCTTCGT CTTCCGAATG
    AACTACAGCC GCAAGAACCA GGACTCGGAA GTTGATGGTG GCATCACCCT TGAGAAGGAA
    ATCTCCAAAG AAGAGCTGGT TGCCGTCCTG GAGCTCTACC GGGAGGCACG GGGGGCCTCC
    TCGGATGTCA CCAGGCTGCT GGAGACCCTC TCCCAGATGG AGAGATACCA GCAACATTCC
    ATGGTGTTTC TGGGACGGCG ATCAAGGACC AAGAGCGACC TGAGCCTGAA GATGTACCAG
    GAGGAGATCC AGGAGTGGTA TGAGGAGCAT GCCAGGGAGC AAGAGCAGCA GCGACAACTC
    AGCAGCAGTG CAGCCCCCGC CGCCCAGCAG CCCCCAGGCA GCCGCCAGCG CTCCCAGACC
    GTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011536791.1
    Translation

    Target ORF information:

    RefSeq Version XM_011538489.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011538489.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAAAGAC TTGGGACTCC CTCTGGGTTG GACTCAACGA AGATGGAGTC TTGCTACATT 
    GCCCAGGCTG GTCTTGAACT GCTAGGCTCA AGCAGTTCTC CCACCTTGAC CTCCCAAAGC
    GCTGAGATTA CAGAAGATGC CTCTAATGGA GGAGTTTCTG AGCAGCACCC CTGGCCCAGT
    GGCTTTGAAA GGGAGCTCAA ACCAGAGACT ATTTCAAGCC CTGGATATCA TATCCTGAGG
    GCCACAGGAG AAGAGAACAT GGCTGTGAGT TTGGATGACG ACGTGCCGCT CATCCTGACC
    TTGGATGAGG GTGGCAGTGC CCCACTGGCT CCCTCCAACG GCCTGGGCCA AGAAGAGCTA
    CCTAGCAAAA ATGGCGGCAG CTATGCCATC CACGACTCCC AGGCCCCCAG TCTCAGCTCT
    GGGGGTGAGA GTTCCCCCTC CAGCCCCGCA CACAACTGGG AGATGAATTA CCAAGAGGCA
    GCAATCTACC TCCAGGAAGG CGAGAACAAC GACAAGTTCT TCACCCACCC CAAGGATGCC
    AAGGCGCTGG CGGCCTACCT CTTTGCACAC AATCACCTCT TCTACCTGAT GGAGCTGGCC
    ACGGCCCTGC TGCTGCTGCT GCTCTCCCTG TGCGAGGCCC CCGCCGTCCC CGCACTCCGG
    CTTGGCATCT ATGTCCACGC CACCCTGGAG CTGTTTGCCC TGATGGTGGT AGTGTTTGAA
    CTCTGCATGA AGTTACGCTG GCTGGGCCTC CACACCTTCA TCCGGCACAA GCGGACCATG
    GTCAAGACCT CGGTGCTGGT GGTGCAGTTT GTCGAGGCCA TCGTGGTGTT GGTACGGCAG
    ATGTCCCATG TGCGGGTGAC CCGAGCACTG CGCTGCATTT TCCTGGTGGA CTGTCGGTAT
    TGCGGTGGCG TCCGGCGCAA CCTGCGGCAG ATCTTCCAGT CCCTGCCGCC CTTCATGGAC
    ATCCTCCTGC TGCTGCTGTT CTTCATGATC ATCTTTGCCA TCCTCGGTTT CTACTTGTTC
    TCCCCTAACC CTTCAGACCC CTACTTCAGC ACCCTGGAGA ACAGCATCGT CAGTCTGTTT
    GTCCTTCTGA CCACAGCCAA TTTCCCAGAT GTGATGATGC CCTCCTACTC CCGGAACCCC
    TGGTCCTGCG TCTTCTTCAT CGTGTACCTC TCCATCGAGC TGTATTTCAT CATGAACCTG
    CTTCTGGCTG TGGTGTTCGA CACCTTCAAT GACATTGAGA AACGCAAGTT CAAGTCTTTG
    CTACTGCACA AGCGAACCGC TATCCAGCAT GCCTACCGCC TGCTCATCAG CCAGAGGAGG
    CCTGCCGGCA TCTCCTACAG GCAGTTTGAA GGCCTCATGC GCTTCTACAA GCCCCGGATG
    AGTGCCAGGG AGCGCTATCT TACCTTCAAG GCCCTGAATC AGAACAACAC ACCCCTGCTC
    AGCCTAAAGG ACTTTTACGA TATCTACGAA GTTGCTGCTT TGAAGTGGAA GGCCAAGAAA
    AACAGAGAGC ACTGGTTTGA TGAGCTTCCC AGGACGGCGC TCCTCATCTT CAAAGGTATT
    AATATCCTTG TGAAGTCCAA GGCCTTCCAG TATTTCATGT ACTTGGTGGT GGCAGTCAAC
    GGGGTCTGGA TCCTCGTGGA GACATTTATG CTGAAAGGTG GGAACTTCTT CTCCAAGCAC
    GTGCCCTGGA GTTACCTCGT CTTTCTAACT ATCTATGGGG TGGAGCTGTT CCTGAAGGTT
    GCCGGCCTGG GCCCTGTGGA GTACTTGTCT TCCGGATGGA ACTTGTTTGA CTTCTCCGTG
    ACAGTGTTCG CCTTCCTGGG ACTGCTGGCG CTGGCCCTCA ACATGGAGCC CTTCTATTTC
    ATCGTGGTCC TGCGCCCCCT CCAGCTGCTG AGGTTGTTTA AGTTGAAGGA GCGCTACCGC
    AACGTGCTGG ACACCATGTT CGAGCTGCTG CCCCGGATGG CCAGCCTGGG CCTCACCCTG
    CTCATCTTTT ACTACTCCTT CGCCATCGTG GGCATGGAGT TCTTCTGCGG GATCGTCTTC
    CCCAACTGCT GCAACACGAG TACAGTGGCA GATGCCTACC GCTGGCGCAA CCACACCGTG
    GGCAACAGGA CCGTGGTGGA GGAAGGCTAC TATTATCTCA ATAATTTTGA CAACATCCTC
    AACAGCTTTG TGACCCTGTT TGAGCTCACA GTTGTCAACA ACTGGTACAT CATCATGGAA
    GGCGTCACCT CTCAGACCTC CCACTGGAGC CGCCTCTACT TCATGACCTT TTACATTGTG
    ACCATGGTGG TGATGACGAT CATTGTCGCC TTTATCCTCG AGGCCTTCGT CTTCCGAATG
    AACTACAGCC GCAAGAACCA GGACTCGGAA GTTGATGGTG GCATCACCCT TGAGAAGGAA
    ATCTCCAAAG AAGAGCTGGT TGCCGTCCTG GAGCTCTACC GGGAGGCACG GGGGGCCTCC
    TCGGATGTCA CCAGGCTGCT GGAGACCCTC TCCCAGATGG AGAGATACCA GCAACATTCC
    ATGGTGTTTC TGGGACGGCG ATCAAGGACC AAGAGCGACC TGAGCCTGAA GATGTACCAG
    GAGGAGATCC AGGAGTGGTA TGAGGAGCAT GCCAGGGAGC AAGAGCAGCA GCGACAACTC
    AGCAGCAGTG CAGCCCCCGC CGCCCAGCAG CCCCCAGGCA GCCGCCAGCG CTCCCAGACC
    GTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu46641
    Clone ID Related Accession (Same CDS sequence) NM_001301214.1 , NM_001351346.1 , XM_011538493.2 , NM_001351347.1 , NM_001351346.2
    Accession Version NM_001301214.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-15
    Organism Homo sapiens(human)
    Product two pore calcium channel protein 1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK296268.1 and BC150203.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus compared to isoform 1. Variants 3, 4, and 5 all encode the same isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296268.1, SRR1660807.164670.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154361, SAMEA2156266 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC
    TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC
    TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT
    AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC
    ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG
    GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC
    ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG
    GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG
    AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG
    AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA
    GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC
    CGCCAGCGCT CCCAGACCGT TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001288143.1
    CDS194..2440
    Translation

    Target ORF information:

    RefSeq Version NM_001301214.1
    Organism Homo sapiens(human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001301214.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC
    TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC
    TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT
    AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC
    ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG
    GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC
    ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG
    GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG
    AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG
    AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA
    GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC
    CGCCAGCGCT CCCAGACCGT TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu46641
    Clone ID Related Accession (Same CDS sequence) NM_001301214.1 , NM_001351346.1 , XM_011538493.2 , NM_001351347.1 , NM_001351346.2
    Accession Version NM_001351346.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-21
    Organism Homo sapiens(human)
    Product two pore calcium channel protein 1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC089999.20 and AC010178.40. On May 6, 2017 this sequence version replaced XM_017019481.1. Transcript Variant: This variant (4), as well as variants 3 and 5, encodes isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.52948.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC
    TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC
    TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT
    AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC
    ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG
    GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC
    ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG
    GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG
    AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG
    AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA
    GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC
    CGCCAGCGCT CCCAGACCGT TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001338275.1
    CDS780..3026
    Translation

    Target ORF information:

    RefSeq Version NM_001351346.1
    Organism Homo sapiens(human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351346.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC
    TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC
    TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT
    AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC
    ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG
    GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC
    ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG
    GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG
    AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG
    AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA
    GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC
    CGCCAGCGCT CCCAGACCGT TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu46641
    Clone ID Related Accession (Same CDS sequence) NM_001301214.1 , NM_001351346.1 , XM_011538493.2 , NM_001351347.1 , NM_001351346.2
    Accession Version NM_001351347.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-16
    Organism Homo sapiens(human)
    Product two pore calcium channel protein 1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC089999.20 and AC010178.40. On May 6, 2017 this sequence version replaced XM_005253906.4. Transcript Variant: This variant (5), as well as variants 3 and 4, encodes isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.215530.1, SRR1660809.229235.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC
    TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC
    TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT
    AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC
    ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG
    GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC
    ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG
    GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG
    AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG
    AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA
    GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC
    CGCCAGCGCT CCCAGACCGT TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001338276.1
    CDS469..2715
    Translation

    Target ORF information:

    RefSeq Version NM_001351347.1
    Organism Homo sapiens(human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351347.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC
    TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC
    TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT
    AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC
    ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG
    GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC
    ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG
    GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG
    AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG
    AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA
    GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC
    CGCCAGCGCT CCCAGACCGT TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu46641
    Clone ID Related Accession (Same CDS sequence) NM_001301214.1 , NM_001351346.1 , XM_011538493.2 , NM_001351347.1 , NM_001351346.2
    Accession Version NM_001351346.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-01
    Organism Homo sapiens(human)
    Product two pore calcium channel protein 1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC089999.20 and AC010178.40. On Jun 2, 2019 this sequence version replaced NM_001351346.1. Transcript Variant: This variant (4), as well as variants 3 and 5, encodes isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.52948.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC
    TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC
    TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT
    AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC
    ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG
    GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC
    ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG
    GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG
    AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG
    AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA
    GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC
    CGCCAGCGCT CCCAGACCGT TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001338275.1
    CDS754..3000
    Translation

    Target ORF information:

    RefSeq Version NM_001351346.2
    Organism Homo sapiens(human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351346.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC
    TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC
    TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT
    AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC
    ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG
    GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC
    ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG
    GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG
    AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG
    AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA
    GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC
    CGCCAGCGCT CCCAGACCGT TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu46641
    Clone ID Related Accession (Same CDS sequence) NM_001301214.1 , NM_001351346.1 , XM_011538493.2 , NM_001351347.1 , NM_001351346.2
    Accession Version XM_011538493.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product two pore calcium channel protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538493.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC
    TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC
    TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT
    AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC
    ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG
    GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC
    ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG
    GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG
    AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG
    AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA
    GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC
    CGCCAGCGCT CCCAGACCGT TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011536795.1
    CDS269..2515
    Translation

    Target ORF information:

    RefSeq Version XM_011538493.2
    Organism Homo sapiens(human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011538493.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC
    TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC
    TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT
    AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC
    ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG
    GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC
    ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG
    GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG
    AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG
    AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA
    GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC
    CGCCAGCGCT CCCAGACCGT TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu31800
    Clone ID Related Accession (Same CDS sequence) NM_001143819.2 , XM_011538490.2 , NM_001143819.3
    Accession Version NM_001143819.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-15
    Organism Homo sapiens(human)
    Product two pore calcium channel protein 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK093490.1, AK057414.1 and BC150203.1. On Jul 25, 2014 this sequence version replaced NM_001143819.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK057414.1, SRR1660807.24185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
    ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG
    CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT
    GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC
    GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC
    CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG
    GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC
    TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC
    TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT
    AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC
    ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG
    GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC
    ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG
    GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG
    AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG
    AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA
    GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC
    CGCCAGCGCT CCCAGACCGT TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137291.1
    CDS192..2858
    Translation

    Target ORF information:

    RefSeq Version NM_001143819.2
    Organism Homo sapiens(human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001143819.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
    ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG
    CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT
    GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC
    GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC
    CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG
    GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC
    TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC
    TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT
    AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC
    ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG
    GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC
    ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG
    GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG
    AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG
    AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA
    GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC
    CGCCAGCGCT CCCAGACCGT TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu31800
    Clone ID Related Accession (Same CDS sequence) NM_001143819.2 , XM_011538490.2 , NM_001143819.3
    Accession Version NM_001143819.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-01
    Organism Homo sapiens(human)
    Product two pore calcium channel protein 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK093490.1, AK057414.1 and BC150203.1. On Jun 2, 2019 this sequence version replaced NM_001143819.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.24185.1, AK057414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
    ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG
    CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT
    GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC
    GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC
    CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG
    GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC
    TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC
    TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT
    AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC
    ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG
    GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC
    ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG
    GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG
    AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG
    AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA
    GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC
    CGCCAGCGCT CCCAGACCGT TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137291.1
    CDS166..2832
    Translation

    Target ORF information:

    RefSeq Version NM_001143819.3
    Organism Homo sapiens(human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001143819.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
    ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG
    CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT
    GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC
    GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC
    CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG
    GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC
    TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC
    TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT
    AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC
    ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG
    GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC
    ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG
    GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG
    AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG
    AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA
    GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC
    CGCCAGCGCT CCCAGACCGT TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu31800
    Clone ID Related Accession (Same CDS sequence) NM_001143819.2 , XM_011538490.2 , NM_001143819.3
    Accession Version XM_011538490.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product two pore calcium channel protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538490.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
    ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG
    CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT
    GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC
    GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC
    CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG
    GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC
    TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC
    TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT
    AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC
    ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG
    GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC
    ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG
    GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG
    AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG
    AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA
    GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC
    CGCCAGCGCT CCCAGACCGT TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011536792.1
    CDS158..2824
    Translation

    Target ORF information:

    RefSeq Version XM_011538490.2
    Organism Homo sapiens(human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011538490.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
    ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG
    CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT
    GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC
    GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC
    CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG
    GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC
    TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC
    TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT
    AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC
    ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG
    GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC
    ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG
    GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG
    AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG
    AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA
    GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC
    CGCCAGCGCT CCCAGACCGT TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83894
    Clone ID Related Accession (Same CDS sequence) XM_017019480.2 , XM_017019480.1
    Accession Version XM_017019480.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2613bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product two pore calcium channel protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017019480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
    ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG
    CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT
    GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC
    GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC
    CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG
    GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGTCTAT
    GGGGTGGAGC TGTTCCTGAA GGTTGCCGGC CTGGGCCCTG TGGAGTACTT GTCTTCCGGA
    TGGAACTTGT TTGACTTCTC CGTGACAGTG TTCGCCTTCC TGGGACTGCT GGCGCTGGCC
    CTCAACATGG AGCCCTTCTA TTTCATCGTG GTCCTGCGCC CCCTCCAGCT GCTGAGGTTG
    TTTAAGTTGA AGGAGCGCTA CCGCAACGTG CTGGACACCA TGTTCGAGCT GCTGCCCCGG
    ATGGCCAGCC TGGGCCTCAC CCTGCTCATC TTTTACTACT CCTTCGCCAT CGTGGGCATG
    GAGTTCTTCT GCGGGATCGT CTTCCCCAAC TGCTGCAACA CGAGTACAGT GGCAGATGCC
    TACCGCTGGC GCAACCACAC CGTGGGCAAC AGGACCGTGG TGGAGGAAGG CTACTATTAT
    CTCAATAATT TTGACAACAT CCTCAACAGC TTTGTGACCC TGTTTGAGCT CACAGTTGTC
    AACAACTGGT ACATCATCAT GGAAGGCGTC ACCTCTCAGA CCTCCCACTG GAGCCGCCTC
    TACTTCATGA CCTTTTACAT TGTGACCATG GTGGTGATGA CGATCATTGT CGCCTTTATC
    CTCGAGGCCT TCGTCTTCCG AATGAACTAC AGCCGCAAGA ACCAGGACTC GGAAGTTGAT
    GGTGGCATCA CCCTTGAGAA GGAAATCTCC AAAGAAGAGC TGGTTGCCGT CCTGGAGCTC
    TACCGGGAGG CACGGGGGGC CTCCTCGGAT GTCACCAGGC TGCTGGAGAC CCTCTCCCAG
    ATGGAGAGAT ACCAGCAACA TTCCATGGTG TTTCTGGGAC GGCGATCAAG GACCAAGAGC
    GACCTGAGCC TGAAGATGTA CCAGGAGGAG ATCCAGGAGT GGTATGAGGA GCATGCCAGG
    GAGCAAGAGC AGCAGCGACA ACTCAGCAGC AGTGCAGCCC CCGCCGCCCA GCAGCCCCCA
    GGCAGCCGCC AGCGCTCCCA GACCGTTACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874969.1
    CDS170..2782
    Translation

    Target ORF information:

    RefSeq Version XM_017019480.2
    Organism Homo sapiens(human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017019480.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
    ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG
    CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT
    GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC
    GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC
    CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG
    GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGTCTAT
    GGGGTGGAGC TGTTCCTGAA GGTTGCCGGC CTGGGCCCTG TGGAGTACTT GTCTTCCGGA
    TGGAACTTGT TTGACTTCTC CGTGACAGTG TTCGCCTTCC TGGGACTGCT GGCGCTGGCC
    CTCAACATGG AGCCCTTCTA TTTCATCGTG GTCCTGCGCC CCCTCCAGCT GCTGAGGTTG
    TTTAAGTTGA AGGAGCGCTA CCGCAACGTG CTGGACACCA TGTTCGAGCT GCTGCCCCGG
    ATGGCCAGCC TGGGCCTCAC CCTGCTCATC TTTTACTACT CCTTCGCCAT CGTGGGCATG
    GAGTTCTTCT GCGGGATCGT CTTCCCCAAC TGCTGCAACA CGAGTACAGT GGCAGATGCC
    TACCGCTGGC GCAACCACAC CGTGGGCAAC AGGACCGTGG TGGAGGAAGG CTACTATTAT
    CTCAATAATT TTGACAACAT CCTCAACAGC TTTGTGACCC TGTTTGAGCT CACAGTTGTC
    AACAACTGGT ACATCATCAT GGAAGGCGTC ACCTCTCAGA CCTCCCACTG GAGCCGCCTC
    TACTTCATGA CCTTTTACAT TGTGACCATG GTGGTGATGA CGATCATTGT CGCCTTTATC
    CTCGAGGCCT TCGTCTTCCG AATGAACTAC AGCCGCAAGA ACCAGGACTC GGAAGTTGAT
    GGTGGCATCA CCCTTGAGAA GGAAATCTCC AAAGAAGAGC TGGTTGCCGT CCTGGAGCTC
    TACCGGGAGG CACGGGGGGC CTCCTCGGAT GTCACCAGGC TGCTGGAGAC CCTCTCCCAG
    ATGGAGAGAT ACCAGCAACA TTCCATGGTG TTTCTGGGAC GGCGATCAAG GACCAAGAGC
    GACCTGAGCC TGAAGATGTA CCAGGAGGAG ATCCAGGAGT GGTATGAGGA GCATGCCAGG
    GAGCAAGAGC AGCAGCGACA ACTCAGCAGC AGTGCAGCCC CCGCCGCCCA GCAGCCCCCA
    GGCAGCCGCC AGCGCTCCCA GACCGTTACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83894
    Clone ID Related Accession (Same CDS sequence) XM_017019480.2 , XM_017019480.1
    Accession Version XM_017019480.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2613bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product two pore calcium channel protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
    ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG
    CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT
    GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC
    GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC
    CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG
    GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGTCTAT
    GGGGTGGAGC TGTTCCTGAA GGTTGCCGGC CTGGGCCCTG TGGAGTACTT GTCTTCCGGA
    TGGAACTTGT TTGACTTCTC CGTGACAGTG TTCGCCTTCC TGGGACTGCT GGCGCTGGCC
    CTCAACATGG AGCCCTTCTA TTTCATCGTG GTCCTGCGCC CCCTCCAGCT GCTGAGGTTG
    TTTAAGTTGA AGGAGCGCTA CCGCAACGTG CTGGACACCA TGTTCGAGCT GCTGCCCCGG
    ATGGCCAGCC TGGGCCTCAC CCTGCTCATC TTTTACTACT CCTTCGCCAT CGTGGGCATG
    GAGTTCTTCT GCGGGATCGT CTTCCCCAAC TGCTGCAACA CGAGTACAGT GGCAGATGCC
    TACCGCTGGC GCAACCACAC CGTGGGCAAC AGGACCGTGG TGGAGGAAGG CTACTATTAT
    CTCAATAATT TTGACAACAT CCTCAACAGC TTTGTGACCC TGTTTGAGCT CACAGTTGTC
    AACAACTGGT ACATCATCAT GGAAGGCGTC ACCTCTCAGA CCTCCCACTG GAGCCGCCTC
    TACTTCATGA CCTTTTACAT TGTGACCATG GTGGTGATGA CGATCATTGT CGCCTTTATC
    CTCGAGGCCT TCGTCTTCCG AATGAACTAC AGCCGCAAGA ACCAGGACTC GGAAGTTGAT
    GGTGGCATCA CCCTTGAGAA GGAAATCTCC AAAGAAGAGC TGGTTGCCGT CCTGGAGCTC
    TACCGGGAGG CACGGGGGGC CTCCTCGGAT GTCACCAGGC TGCTGGAGAC CCTCTCCCAG
    ATGGAGAGAT ACCAGCAACA TTCCATGGTG TTTCTGGGAC GGCGATCAAG GACCAAGAGC
    GACCTGAGCC TGAAGATGTA CCAGGAGGAG ATCCAGGAGT GGTATGAGGA GCATGCCAGG
    GAGCAAGAGC AGCAGCGACA ACTCAGCAGC AGTGCAGCCC CCGCCGCCCA GCAGCCCCCA
    GGCAGCCGCC AGCGCTCCCA GACCGTTACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874969.1
    CDS118..2730
    Translation

    Target ORF information:

    RefSeq Version XM_017019480.1
    Organism Homo sapiens(human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017019480.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
    ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG
    CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT
    GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC
    GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC
    CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG
    GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGTCTAT
    GGGGTGGAGC TGTTCCTGAA GGTTGCCGGC CTGGGCCCTG TGGAGTACTT GTCTTCCGGA
    TGGAACTTGT TTGACTTCTC CGTGACAGTG TTCGCCTTCC TGGGACTGCT GGCGCTGGCC
    CTCAACATGG AGCCCTTCTA TTTCATCGTG GTCCTGCGCC CCCTCCAGCT GCTGAGGTTG
    TTTAAGTTGA AGGAGCGCTA CCGCAACGTG CTGGACACCA TGTTCGAGCT GCTGCCCCGG
    ATGGCCAGCC TGGGCCTCAC CCTGCTCATC TTTTACTACT CCTTCGCCAT CGTGGGCATG
    GAGTTCTTCT GCGGGATCGT CTTCCCCAAC TGCTGCAACA CGAGTACAGT GGCAGATGCC
    TACCGCTGGC GCAACCACAC CGTGGGCAAC AGGACCGTGG TGGAGGAAGG CTACTATTAT
    CTCAATAATT TTGACAACAT CCTCAACAGC TTTGTGACCC TGTTTGAGCT CACAGTTGTC
    AACAACTGGT ACATCATCAT GGAAGGCGTC ACCTCTCAGA CCTCCCACTG GAGCCGCCTC
    TACTTCATGA CCTTTTACAT TGTGACCATG GTGGTGATGA CGATCATTGT CGCCTTTATC
    CTCGAGGCCT TCGTCTTCCG AATGAACTAC AGCCGCAAGA ACCAGGACTC GGAAGTTGAT
    GGTGGCATCA CCCTTGAGAA GGAAATCTCC AAAGAAGAGC TGGTTGCCGT CCTGGAGCTC
    TACCGGGAGG CACGGGGGGC CTCCTCGGAT GTCACCAGGC TGCTGGAGAC CCTCTCCCAG
    ATGGAGAGAT ACCAGCAACA TTCCATGGTG TTTCTGGGAC GGCGATCAAG GACCAAGAGC
    GACCTGAGCC TGAAGATGTA CCAGGAGGAG ATCCAGGAGT GGTATGAGGA GCATGCCAGG
    GAGCAAGAGC AGCAGCGACA ACTCAGCAGC AGTGCAGCCC CCGCCGCCCA GCAGCCCCCA
    GGCAGCCGCC AGCGCTCCCA GACCGTTACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83895
    Clone ID Related Accession (Same CDS sequence) XM_017019482.2 , XM_017019482.1
    Accession Version XM_017019482.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product two pore calcium channel protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017019482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
    ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG
    CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT
    GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC
    GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC
    CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG
    GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874971.1
    CDS170..2095
    Translation

    Target ORF information:

    RefSeq Version XM_017019482.2
    Organism Homo sapiens(human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017019482.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
    ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG
    CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT
    GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC
    GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC
    CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG
    GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83895
    Clone ID Related Accession (Same CDS sequence) XM_017019482.2 , XM_017019482.1
    Accession Version XM_017019482.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product two pore calcium channel protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
    ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG
    CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT
    GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC
    GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC
    CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG
    GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874971.1
    CDS119..2044
    Translation

    Target ORF information:

    RefSeq Version XM_017019482.1
    Organism Homo sapiens(human)
    Definition Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017019482.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
    ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG
    CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT
    GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC
    GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC
    CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG
    GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG
    ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC
    ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC
    TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC
    GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG
    ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC
    CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC
    GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC
    CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC
    CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG
    TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA
    CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG
    CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC
    TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG
    AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG
    AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC
    CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC
    TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG
    AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG
    GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC
    TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC
    ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG
    TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC
    AGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.