TPCN1 cDNA ORF clone, Bos taurus(cattle)

The following TPCN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TPCN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa09064 XM_005218006.3
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa09064 XM_010823416.2
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa09064 XM_015467155.1
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa09064 XM_010814053.3
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa09065 XM_024977760.1
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09065 XM_024977761.1
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09065 XM_588037.9
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09065 XM_002694517.6
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa47815 XM_010814054.3
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa47815 XM_010823418.2
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa47815 XM_024977762.1
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09063 XM_005218006.4
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa09063 XM_024977758.1
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa09063 XM_024977757.1
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa09066 XM_024977759.1
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa93226 XM_015467156.1
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa93226 XM_015475525.1
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa93227 XM_015475526.2
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa93227 XM_015467157.1
Latest version!
Bos taurus two pore segment channel 1 (TPCN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OBa47815 XM_010814054.2 Bos taurus two pore segment channel 1 (TPCN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09064 XM_010814053.2 Bos taurus two pore segment channel 1 (TPCN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa93227 XM_015475526.1 Bos taurus two pore segment channel 1 (TPCN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa09065 XM_002694517.5 Bos taurus two pore segment channel 1 (TPCN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa09064
    Clone ID Related Accession (Same CDS sequence) XM_010823416.2 , XM_015467155.1 , XM_005218006.3 , XM_010814053.3 , XM_010814053.2
    Accession Version XM_010823416.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823416.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACTGCCTCC
    CAGGAAAGGC GGAAATGCAG AGGCGAGGGC CAGCCATCAT CTTCCTGGAG GAGGCAGCAG
    AGGGCCAGCT CCCTGACAGC CCCATGGAGA GGTCATCCCG TCTCCTCCAG CTGGGCCCTT
    GTGGTATGCC TTGACCTGGC CGTGGATGGA GGCAAATATA CCGTCCACGA CTCCCAGGCC
    CCCAGTCTGA GCTTAGAGGG TGAGAACTCC CCCTCCAGCC CCACTGAACA TGACTGGGAG
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821718.1
    CDS343..2946
    Translation

    Target ORF information:

    RefSeq Version XM_010823416.2
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823416.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACTGCCTCC
    CAGGAAAGGC GGAAATGCAG AGGCGAGGGC CAGCCATCAT CTTCCTGGAG GAGGCAGCAG
    AGGGCCAGCT CCCTGACAGC CCCATGGAGA GGTCATCCCG TCTCCTCCAG CTGGGCCCTT
    GTGGTATGCC TTGACCTGGC CGTGGATGGA GGCAAATATA CCGTCCACGA CTCCCAGGCC
    CCCAGTCTGA GCTTAGAGGG TGAGAACTCC CCCTCCAGCC CCACTGAACA TGACTGGGAG
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09064
    Clone ID Related Accession (Same CDS sequence) XM_010823416.2 , XM_015467155.1 , XM_005218006.3 , XM_010814053.3 , XM_010814053.2
    Accession Version XM_015467155.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACTGCCTCC
    CAGGAAAGGC GGAAATGCAG AGGCGAGGGC CAGCCATCAT CTTCCTGGAG GAGGCAGCAG
    AGGGCCAGCT CCCTGACAGC CCCATGGAGA GGTCATCCCG TCTCCTCCAG CTGGGCCCTT
    GTGGTATGCC TTGACCTGGC CGTGGATGGA GGCAAATATA CCGTCCACGA CTCCCAGGCC
    CCCAGTCTGA GCTTAGAGGG TGAGAACTCC CCCTCCAGCC CCACTGAACA TGACTGGGAG
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322641.1
    CDS297..2900
    Translation

    Target ORF information:

    RefSeq Version XM_015467155.1
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467155.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACTGCCTCC
    CAGGAAAGGC GGAAATGCAG AGGCGAGGGC CAGCCATCAT CTTCCTGGAG GAGGCAGCAG
    AGGGCCAGCT CCCTGACAGC CCCATGGAGA GGTCATCCCG TCTCCTCCAG CTGGGCCCTT
    GTGGTATGCC TTGACCTGGC CGTGGATGGA GGCAAATATA CCGTCCACGA CTCCCAGGCC
    CCCAGTCTGA GCTTAGAGGG TGAGAACTCC CCCTCCAGCC CCACTGAACA TGACTGGGAG
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09064
    Clone ID Related Accession (Same CDS sequence) XM_010823416.2 , XM_015467155.1 , XM_005218006.3 , XM_010814053.3 , XM_010814053.2
    Accession Version XM_005218006.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218006.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACTGCCTCC
    CAGGAAAGGC GGAAATGCAG AGGCGAGGGC CAGCCATCAT CTTCCTGGAG GAGGCAGCAG
    AGGGCCAGCT CCCTGACAGC CCCATGGAGA GGTCATCCCG TCTCCTCCAG CTGGGCCCTT
    GTGGTATGCC TTGACCTGGC CGTGGATGGA GGCAAATATA CCGTCCACGA CTCCCAGGCC
    CCCAGTCTGA GCTTAGAGGG TGAGAACTCC CCCTCCAGCC CCACTGAACA TGACTGGGAG
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218063.1
    CDS297..2900
    Translation

    Target ORF information:

    RefSeq Version XM_005218006.3
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218006.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACTGCCTCC
    CAGGAAAGGC GGAAATGCAG AGGCGAGGGC CAGCCATCAT CTTCCTGGAG GAGGCAGCAG
    AGGGCCAGCT CCCTGACAGC CCCATGGAGA GGTCATCCCG TCTCCTCCAG CTGGGCCCTT
    GTGGTATGCC TTGACCTGGC CGTGGATGGA GGCAAATATA CCGTCCACGA CTCCCAGGCC
    CCCAGTCTGA GCTTAGAGGG TGAGAACTCC CCCTCCAGCC CCACTGAACA TGACTGGGAG
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09064
    Clone ID Related Accession (Same CDS sequence) XM_010823416.2 , XM_015467155.1 , XM_005218006.3 , XM_010814053.3 , XM_010814053.2
    Accession Version XM_010814053.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814053.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACTGCCTCC
    CAGGAAAGGC GGAAATGCAG AGGCGAGGGC CAGCCATCAT CTTCCTGGAG GAGGCAGCAG
    AGGGCCAGCT CCCTGACAGC CCCATGGAGA GGTCATCCCG TCTCCTCCAG CTGGGCCCTT
    GTGGTATGCC TTGACCTGGC CGTGGATGGA GGCAAATATA CCGTCCACGA CTCCCAGGCC
    CCCAGTCTGA GCTTAGAGGG TGAGAACTCC CCCTCCAGCC CCACTGAACA TGACTGGGAG
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812355.1
    CDS351..2954
    Translation

    Target ORF information:

    RefSeq Version XM_010814053.3
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814053.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACTGCCTCC
    CAGGAAAGGC GGAAATGCAG AGGCGAGGGC CAGCCATCAT CTTCCTGGAG GAGGCAGCAG
    AGGGCCAGCT CCCTGACAGC CCCATGGAGA GGTCATCCCG TCTCCTCCAG CTGGGCCCTT
    GTGGTATGCC TTGACCTGGC CGTGGATGGA GGCAAATATA CCGTCCACGA CTCCCAGGCC
    CCCAGTCTGA GCTTAGAGGG TGAGAACTCC CCCTCCAGCC CCACTGAACA TGACTGGGAG
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09064
    Clone ID Related Accession (Same CDS sequence) XM_010823416.2 , XM_015467155.1 , XM_005218006.3 , XM_010814053.3 , XM_010814053.2
    Accession Version XM_010814053.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814053.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACTGCCTCC
    CAGGAAAGGC GGAAATGCAG AGGCGAGGGC CAGCCATCAT CTTCCTGGAG GAGGCAGCAG
    AGGGCCAGCT CCCTGACAGC CCCATGGAGA GGTCATCCCG TCTCCTCCAG CTGGGCCCTT
    GTGGTATGCC TTGACCTGGC CGTGGATGGA GGCAAATATA CCGTCCACGA CTCCCAGGCC
    CCCAGTCTGA GCTTAGAGGG TGAGAACTCC CCCTCCAGCC CCACTGAACA TGACTGGGAG
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812355.1
    CDS343..2946
    Translation

    Target ORF information:

    RefSeq Version XM_010814053.2
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814053.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACTGCCTCC
    CAGGAAAGGC GGAAATGCAG AGGCGAGGGC CAGCCATCAT CTTCCTGGAG GAGGCAGCAG
    AGGGCCAGCT CCCTGACAGC CCCATGGAGA GGTCATCCCG TCTCCTCCAG CTGGGCCCTT
    GTGGTATGCC TTGACCTGGC CGTGGATGGA GGCAAATATA CCGTCCACGA CTCCCAGGCC
    CCCAGTCTGA GCTTAGAGGG TGAGAACTCC CCCTCCAGCC CCACTGAACA TGACTGGGAG
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09065
    Clone ID Related Accession (Same CDS sequence) XM_024977760.1 , XM_588037.9 , XM_002694517.6 , XM_024977761.1 , XM_002694517.5
    Accession Version XM_024977760.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AAATGGAGGC
    AAATATACCG TCCACGACTC CCAGGCCCCC AGTCTGAGCT TAGAGGGTGA GAACTCCCCC
    TCCAGCCCCA CTGAACATGA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG
    GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC TCTGGCGGCC
    TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCGCGGC CTTGCTCCTG
    CTGCTGCTCT CCCTGTGCGA GGCTCCCGCC GTCCCCGCGC TCCGGCTGGG CATTTACGTC
    CACGCAACCC TGGAGCTGTT CGCCCTGGTG GTGGTCGTGT TTGAACTCTG CATGAAGTTG
    CGGTGGCTGG GCCTTCACAC CTTCATCCGG CACAGAAGGA CCATGGTCAA GACCTCCGTG
    CTGGTGGTGC AGTTCGTGGA GGCCATCGTG GTGTTGGTAC GGCAGACGTC ACACATGCGG
    GTGACCCGGG CGCTGCGCTG CATCTTCCTG GTGGACTGTC GGTACTGCGG CGGCGTCCGG
    CGCAACCTGC GTCAGATGTT TCAGTCCCTG CCGCCCTTCA TGGACATCCT CCTGCTTCTC
    CTCTTCTTCA TGATCATTTT CGCCATCCTC GGTTTCTACT TATTCTCCCC TAATCCTTCA
    GACCCCTACT TCAGCACCCT GGAGAACAGC ATCATCAGTC TGTTTGTCCT TCTGACCACC
    GCCAACTTCC CAGACGTGAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC
    TTCATCGTGT ACCTCTCCAT TGAGCTGTAC TTTATCATGA ACCTGCTCCT GGCCGTGGTA
    TTTGACACCT TCAATGACAT CGAGAAACGC AAGTTCAAGT CTTTGCTGCT ACACAAGCGA
    ACTGCCATCC AGCACGCCTA CCGCCTGCTT GTCAGCCAGC GGAGGCCTGC CGGCATCTCC
    TACAGGCAGT TCGAAGGCCT GATGCGTTTC TACAAGCCCC GGATGAGTGC GGGGGAGCGC
    TATCTCACCT TCAAGGCCCT GAATCAGAGC AACACGCCAC TGCTCAGCCT GAAGGACTTT
    CATGATATTT ATGAAGTTGC CGCCTTAAAG TGGAAGGCAA AGAGAAATAG AGAACACTGG
    TTTGATGAGC TTCCCAGGAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAG
    TCCAAGGCCT TCCAGTATTT CATGTATTTG GTGGTAGCGG TCAACGGGGT CTGGATCCTC
    GTGGAGACCT TCATGCTGAA AGGTGGGAAC TTCGTCTCCA GGCACGTGCC GTGGAGTCAC
    CTCGTCTTTC TGACTATCTA TGCGGTAGAG CTGTTCCTGA AAGTTGCCGG CCTGGGCCCC
    ATTGAGTACC TGTCCTCAGG ATGGAACCTG TTCGACTTCT CCGTCACAGC CTTTGCCTTC
    CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTCT ACTTCATAGT GGTCCTGCGT
    CCCCTCCAGC TGCTGAGGTT GTTTAAGCTG AAGAAGCGTT ACCGCAACGT GCTGGACACC
    ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTGA CTCTGCTCAT CTTCTACTAC
    TCCTTCGCCA TCGTGGGCAT GGAATTCTTC TATGGAATCC TCTACCCCAA CTGCTGCAAT
    ACAAGCACAG TGGCAGACGC CTACCGCTGG CTGAACCACT CCGTGGGCAA CAGGACCGTC
    GTGGAGGAGG GCTATTACTA TCTCAATAAT TTTGACAACA TCCTGAACAG CTTTGTGACC
    TTGTTCGAGC TCACGGTTGT CAACAACTGG TACATCATCA TGGAAGGTGT CACCTCCCAG
    ACCTCGCACT GGAGCCGCCT CTACTTCATG ACCTTCTACA TCGTGACCAT GGTGGTGATG
    ACCATCATTG TGGCCTTCAT CCTCGAAGCC TTTGTCTTCC GAATGAACTA CAGCCGCAAG
    AACCAGGACT CGGAAGTGGA CGACGGCATC ACCCTGGAGA AGGAGCTCTC CAAAGACGAG
    CTGGTCGCAG TTCTGAAGCT CTACCGGGAG GCAGGGGGAG CCAACTCAGA CATTGCCCAG
    CTGCTCAAGA TCCTCTCCCA GCTGGAGAGA TACGAGCAAA ATACCTTGGT GTTTCTGGGA
    CGGAGATCAA GGACCAAAAG CGACCTGAGC CTAAAGATGT ACCAAGAGGA GATTCAGGAG
    TGGTATGAGG AACATGCCCG CAAGCAGGAG GAACGACAGC TCAGCAGCTG CGTGGTCCCT
    GCCACCCAGC AGCCTCCAGG CAGCCGCCAG CGCTCCCAGA CCATCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833528.1
    CDS346..2796
    Translation

    Target ORF information:

    RefSeq Version XM_024977760.1
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977760.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AAATGGAGGC
    AAATATACCG TCCACGACTC CCAGGCCCCC AGTCTGAGCT TAGAGGGTGA GAACTCCCCC
    TCCAGCCCCA CTGAACATGA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG
    GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC TCTGGCGGCC
    TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCGCGGC CTTGCTCCTG
    CTGCTGCTCT CCCTGTGCGA GGCTCCCGCC GTCCCCGCGC TCCGGCTGGG CATTTACGTC
    CACGCAACCC TGGAGCTGTT CGCCCTGGTG GTGGTCGTGT TTGAACTCTG CATGAAGTTG
    CGGTGGCTGG GCCTTCACAC CTTCATCCGG CACAGAAGGA CCATGGTCAA GACCTCCGTG
    CTGGTGGTGC AGTTCGTGGA GGCCATCGTG GTGTTGGTAC GGCAGACGTC ACACATGCGG
    GTGACCCGGG CGCTGCGCTG CATCTTCCTG GTGGACTGTC GGTACTGCGG CGGCGTCCGG
    CGCAACCTGC GTCAGATGTT TCAGTCCCTG CCGCCCTTCA TGGACATCCT CCTGCTTCTC
    CTCTTCTTCA TGATCATTTT CGCCATCCTC GGTTTCTACT TATTCTCCCC TAATCCTTCA
    GACCCCTACT TCAGCACCCT GGAGAACAGC ATCATCAGTC TGTTTGTCCT TCTGACCACC
    GCCAACTTCC CAGACGTGAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC
    TTCATCGTGT ACCTCTCCAT TGAGCTGTAC TTTATCATGA ACCTGCTCCT GGCCGTGGTA
    TTTGACACCT TCAATGACAT CGAGAAACGC AAGTTCAAGT CTTTGCTGCT ACACAAGCGA
    ACTGCCATCC AGCACGCCTA CCGCCTGCTT GTCAGCCAGC GGAGGCCTGC CGGCATCTCC
    TACAGGCAGT TCGAAGGCCT GATGCGTTTC TACAAGCCCC GGATGAGTGC GGGGGAGCGC
    TATCTCACCT TCAAGGCCCT GAATCAGAGC AACACGCCAC TGCTCAGCCT GAAGGACTTT
    CATGATATTT ATGAAGTTGC CGCCTTAAAG TGGAAGGCAA AGAGAAATAG AGAACACTGG
    TTTGATGAGC TTCCCAGGAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAG
    TCCAAGGCCT TCCAGTATTT CATGTATTTG GTGGTAGCGG TCAACGGGGT CTGGATCCTC
    GTGGAGACCT TCATGCTGAA AGGTGGGAAC TTCGTCTCCA GGCACGTGCC GTGGAGTCAC
    CTCGTCTTTC TGACTATCTA TGCGGTAGAG CTGTTCCTGA AAGTTGCCGG CCTGGGCCCC
    ATTGAGTACC TGTCCTCAGG ATGGAACCTG TTCGACTTCT CCGTCACAGC CTTTGCCTTC
    CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTCT ACTTCATAGT GGTCCTGCGT
    CCCCTCCAGC TGCTGAGGTT GTTTAAGCTG AAGAAGCGTT ACCGCAACGT GCTGGACACC
    ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTGA CTCTGCTCAT CTTCTACTAC
    TCCTTCGCCA TCGTGGGCAT GGAATTCTTC TATGGAATCC TCTACCCCAA CTGCTGCAAT
    ACAAGCACAG TGGCAGACGC CTACCGCTGG CTGAACCACT CCGTGGGCAA CAGGACCGTC
    GTGGAGGAGG GCTATTACTA TCTCAATAAT TTTGACAACA TCCTGAACAG CTTTGTGACC
    TTGTTCGAGC TCACGGTTGT CAACAACTGG TACATCATCA TGGAAGGTGT CACCTCCCAG
    ACCTCGCACT GGAGCCGCCT CTACTTCATG ACCTTCTACA TCGTGACCAT GGTGGTGATG
    ACCATCATTG TGGCCTTCAT CCTCGAAGCC TTTGTCTTCC GAATGAACTA CAGCCGCAAG
    AACCAGGACT CGGAAGTGGA CGACGGCATC ACCCTGGAGA AGGAGCTCTC CAAAGACGAG
    CTGGTCGCAG TTCTGAAGCT CTACCGGGAG GCAGGGGGAG CCAACTCAGA CATTGCCCAG
    CTGCTCAAGA TCCTCTCCCA GCTGGAGAGA TACGAGCAAA ATACCTTGGT GTTTCTGGGA
    CGGAGATCAA GGACCAAAAG CGACCTGAGC CTAAAGATGT ACCAAGAGGA GATTCAGGAG
    TGGTATGAGG AACATGCCCG CAAGCAGGAG GAACGACAGC TCAGCAGCTG CGTGGTCCCT
    GCCACCCAGC AGCCTCCAGG CAGCCGCCAG CGCTCCCAGA CCATCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09065
    Clone ID Related Accession (Same CDS sequence) XM_024977760.1 , XM_588037.9 , XM_002694517.6 , XM_024977761.1 , XM_002694517.5
    Accession Version XM_588037.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_588037.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AAATGGAGGC
    AAATATACCG TCCACGACTC CCAGGCCCCC AGTCTGAGCT TAGAGGGTGA GAACTCCCCC
    TCCAGCCCCA CTGAACATGA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG
    GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC TCTGGCGGCC
    TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCGCGGC CTTGCTCCTG
    CTGCTGCTCT CCCTGTGCGA GGCTCCCGCC GTCCCCGCGC TCCGGCTGGG CATTTACGTC
    CACGCAACCC TGGAGCTGTT CGCCCTGGTG GTGGTCGTGT TTGAACTCTG CATGAAGTTG
    CGGTGGCTGG GCCTTCACAC CTTCATCCGG CACAGAAGGA CCATGGTCAA GACCTCCGTG
    CTGGTGGTGC AGTTCGTGGA GGCCATCGTG GTGTTGGTAC GGCAGACGTC ACACATGCGG
    GTGACCCGGG CGCTGCGCTG CATCTTCCTG GTGGACTGTC GGTACTGCGG CGGCGTCCGG
    CGCAACCTGC GTCAGATGTT TCAGTCCCTG CCGCCCTTCA TGGACATCCT CCTGCTTCTC
    CTCTTCTTCA TGATCATTTT CGCCATCCTC GGTTTCTACT TATTCTCCCC TAATCCTTCA
    GACCCCTACT TCAGCACCCT GGAGAACAGC ATCATCAGTC TGTTTGTCCT TCTGACCACC
    GCCAACTTCC CAGACGTGAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC
    TTCATCGTGT ACCTCTCCAT TGAGCTGTAC TTTATCATGA ACCTGCTCCT GGCCGTGGTA
    TTTGACACCT TCAATGACAT CGAGAAACGC AAGTTCAAGT CTTTGCTGCT ACACAAGCGA
    ACTGCCATCC AGCACGCCTA CCGCCTGCTT GTCAGCCAGC GGAGGCCTGC CGGCATCTCC
    TACAGGCAGT TCGAAGGCCT GATGCGTTTC TACAAGCCCC GGATGAGTGC GGGGGAGCGC
    TATCTCACCT TCAAGGCCCT GAATCAGAGC AACACGCCAC TGCTCAGCCT GAAGGACTTT
    CATGATATTT ATGAAGTTGC CGCCTTAAAG TGGAAGGCAA AGAGAAATAG AGAACACTGG
    TTTGATGAGC TTCCCAGGAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAG
    TCCAAGGCCT TCCAGTATTT CATGTATTTG GTGGTAGCGG TCAACGGGGT CTGGATCCTC
    GTGGAGACCT TCATGCTGAA AGGTGGGAAC TTCGTCTCCA GGCACGTGCC GTGGAGTCAC
    CTCGTCTTTC TGACTATCTA TGCGGTAGAG CTGTTCCTGA AAGTTGCCGG CCTGGGCCCC
    ATTGAGTACC TGTCCTCAGG ATGGAACCTG TTCGACTTCT CCGTCACAGC CTTTGCCTTC
    CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTCT ACTTCATAGT GGTCCTGCGT
    CCCCTCCAGC TGCTGAGGTT GTTTAAGCTG AAGAAGCGTT ACCGCAACGT GCTGGACACC
    ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTGA CTCTGCTCAT CTTCTACTAC
    TCCTTCGCCA TCGTGGGCAT GGAATTCTTC TATGGAATCC TCTACCCCAA CTGCTGCAAT
    ACAAGCACAG TGGCAGACGC CTACCGCTGG CTGAACCACT CCGTGGGCAA CAGGACCGTC
    GTGGAGGAGG GCTATTACTA TCTCAATAAT TTTGACAACA TCCTGAACAG CTTTGTGACC
    TTGTTCGAGC TCACGGTTGT CAACAACTGG TACATCATCA TGGAAGGTGT CACCTCCCAG
    ACCTCGCACT GGAGCCGCCT CTACTTCATG ACCTTCTACA TCGTGACCAT GGTGGTGATG
    ACCATCATTG TGGCCTTCAT CCTCGAAGCC TTTGTCTTCC GAATGAACTA CAGCCGCAAG
    AACCAGGACT CGGAAGTGGA CGACGGCATC ACCCTGGAGA AGGAGCTCTC CAAAGACGAG
    CTGGTCGCAG TTCTGAAGCT CTACCGGGAG GCAGGGGGAG CCAACTCAGA CATTGCCCAG
    CTGCTCAAGA TCCTCTCCCA GCTGGAGAGA TACGAGCAAA ATACCTTGGT GTTTCTGGGA
    CGGAGATCAA GGACCAAAAG CGACCTGAGC CTAAAGATGT ACCAAGAGGA GATTCAGGAG
    TGGTATGAGG AACATGCCCG CAAGCAGGAG GAACGACAGC TCAGCAGCTG CGTGGTCCCT
    GCCACCCAGC AGCCTCCAGG CAGCCGCCAG CGCTCCCAGA CCATCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_588037.2
    CDS342..2792
    Translation

    Target ORF information:

    RefSeq Version XM_588037.9
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_588037.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AAATGGAGGC
    AAATATACCG TCCACGACTC CCAGGCCCCC AGTCTGAGCT TAGAGGGTGA GAACTCCCCC
    TCCAGCCCCA CTGAACATGA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG
    GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC TCTGGCGGCC
    TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCGCGGC CTTGCTCCTG
    CTGCTGCTCT CCCTGTGCGA GGCTCCCGCC GTCCCCGCGC TCCGGCTGGG CATTTACGTC
    CACGCAACCC TGGAGCTGTT CGCCCTGGTG GTGGTCGTGT TTGAACTCTG CATGAAGTTG
    CGGTGGCTGG GCCTTCACAC CTTCATCCGG CACAGAAGGA CCATGGTCAA GACCTCCGTG
    CTGGTGGTGC AGTTCGTGGA GGCCATCGTG GTGTTGGTAC GGCAGACGTC ACACATGCGG
    GTGACCCGGG CGCTGCGCTG CATCTTCCTG GTGGACTGTC GGTACTGCGG CGGCGTCCGG
    CGCAACCTGC GTCAGATGTT TCAGTCCCTG CCGCCCTTCA TGGACATCCT CCTGCTTCTC
    CTCTTCTTCA TGATCATTTT CGCCATCCTC GGTTTCTACT TATTCTCCCC TAATCCTTCA
    GACCCCTACT TCAGCACCCT GGAGAACAGC ATCATCAGTC TGTTTGTCCT TCTGACCACC
    GCCAACTTCC CAGACGTGAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC
    TTCATCGTGT ACCTCTCCAT TGAGCTGTAC TTTATCATGA ACCTGCTCCT GGCCGTGGTA
    TTTGACACCT TCAATGACAT CGAGAAACGC AAGTTCAAGT CTTTGCTGCT ACACAAGCGA
    ACTGCCATCC AGCACGCCTA CCGCCTGCTT GTCAGCCAGC GGAGGCCTGC CGGCATCTCC
    TACAGGCAGT TCGAAGGCCT GATGCGTTTC TACAAGCCCC GGATGAGTGC GGGGGAGCGC
    TATCTCACCT TCAAGGCCCT GAATCAGAGC AACACGCCAC TGCTCAGCCT GAAGGACTTT
    CATGATATTT ATGAAGTTGC CGCCTTAAAG TGGAAGGCAA AGAGAAATAG AGAACACTGG
    TTTGATGAGC TTCCCAGGAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAG
    TCCAAGGCCT TCCAGTATTT CATGTATTTG GTGGTAGCGG TCAACGGGGT CTGGATCCTC
    GTGGAGACCT TCATGCTGAA AGGTGGGAAC TTCGTCTCCA GGCACGTGCC GTGGAGTCAC
    CTCGTCTTTC TGACTATCTA TGCGGTAGAG CTGTTCCTGA AAGTTGCCGG CCTGGGCCCC
    ATTGAGTACC TGTCCTCAGG ATGGAACCTG TTCGACTTCT CCGTCACAGC CTTTGCCTTC
    CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTCT ACTTCATAGT GGTCCTGCGT
    CCCCTCCAGC TGCTGAGGTT GTTTAAGCTG AAGAAGCGTT ACCGCAACGT GCTGGACACC
    ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTGA CTCTGCTCAT CTTCTACTAC
    TCCTTCGCCA TCGTGGGCAT GGAATTCTTC TATGGAATCC TCTACCCCAA CTGCTGCAAT
    ACAAGCACAG TGGCAGACGC CTACCGCTGG CTGAACCACT CCGTGGGCAA CAGGACCGTC
    GTGGAGGAGG GCTATTACTA TCTCAATAAT TTTGACAACA TCCTGAACAG CTTTGTGACC
    TTGTTCGAGC TCACGGTTGT CAACAACTGG TACATCATCA TGGAAGGTGT CACCTCCCAG
    ACCTCGCACT GGAGCCGCCT CTACTTCATG ACCTTCTACA TCGTGACCAT GGTGGTGATG
    ACCATCATTG TGGCCTTCAT CCTCGAAGCC TTTGTCTTCC GAATGAACTA CAGCCGCAAG
    AACCAGGACT CGGAAGTGGA CGACGGCATC ACCCTGGAGA AGGAGCTCTC CAAAGACGAG
    CTGGTCGCAG TTCTGAAGCT CTACCGGGAG GCAGGGGGAG CCAACTCAGA CATTGCCCAG
    CTGCTCAAGA TCCTCTCCCA GCTGGAGAGA TACGAGCAAA ATACCTTGGT GTTTCTGGGA
    CGGAGATCAA GGACCAAAAG CGACCTGAGC CTAAAGATGT ACCAAGAGGA GATTCAGGAG
    TGGTATGAGG AACATGCCCG CAAGCAGGAG GAACGACAGC TCAGCAGCTG CGTGGTCCCT
    GCCACCCAGC AGCCTCCAGG CAGCCGCCAG CGCTCCCAGA CCATCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09065
    Clone ID Related Accession (Same CDS sequence) XM_024977760.1 , XM_588037.9 , XM_002694517.6 , XM_024977761.1 , XM_002694517.5
    Accession Version XM_002694517.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002694517.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AAATGGAGGC
    AAATATACCG TCCACGACTC CCAGGCCCCC AGTCTGAGCT TAGAGGGTGA GAACTCCCCC
    TCCAGCCCCA CTGAACATGA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG
    GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC TCTGGCGGCC
    TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCGCGGC CTTGCTCCTG
    CTGCTGCTCT CCCTGTGCGA GGCTCCCGCC GTCCCCGCGC TCCGGCTGGG CATTTACGTC
    CACGCAACCC TGGAGCTGTT CGCCCTGGTG GTGGTCGTGT TTGAACTCTG CATGAAGTTG
    CGGTGGCTGG GCCTTCACAC CTTCATCCGG CACAGAAGGA CCATGGTCAA GACCTCCGTG
    CTGGTGGTGC AGTTCGTGGA GGCCATCGTG GTGTTGGTAC GGCAGACGTC ACACATGCGG
    GTGACCCGGG CGCTGCGCTG CATCTTCCTG GTGGACTGTC GGTACTGCGG CGGCGTCCGG
    CGCAACCTGC GTCAGATGTT TCAGTCCCTG CCGCCCTTCA TGGACATCCT CCTGCTTCTC
    CTCTTCTTCA TGATCATTTT CGCCATCCTC GGTTTCTACT TATTCTCCCC TAATCCTTCA
    GACCCCTACT TCAGCACCCT GGAGAACAGC ATCATCAGTC TGTTTGTCCT TCTGACCACC
    GCCAACTTCC CAGACGTGAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC
    TTCATCGTGT ACCTCTCCAT TGAGCTGTAC TTTATCATGA ACCTGCTCCT GGCCGTGGTA
    TTTGACACCT TCAATGACAT CGAGAAACGC AAGTTCAAGT CTTTGCTGCT ACACAAGCGA
    ACTGCCATCC AGCACGCCTA CCGCCTGCTT GTCAGCCAGC GGAGGCCTGC CGGCATCTCC
    TACAGGCAGT TCGAAGGCCT GATGCGTTTC TACAAGCCCC GGATGAGTGC GGGGGAGCGC
    TATCTCACCT TCAAGGCCCT GAATCAGAGC AACACGCCAC TGCTCAGCCT GAAGGACTTT
    CATGATATTT ATGAAGTTGC CGCCTTAAAG TGGAAGGCAA AGAGAAATAG AGAACACTGG
    TTTGATGAGC TTCCCAGGAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAG
    TCCAAGGCCT TCCAGTATTT CATGTATTTG GTGGTAGCGG TCAACGGGGT CTGGATCCTC
    GTGGAGACCT TCATGCTGAA AGGTGGGAAC TTCGTCTCCA GGCACGTGCC GTGGAGTCAC
    CTCGTCTTTC TGACTATCTA TGCGGTAGAG CTGTTCCTGA AAGTTGCCGG CCTGGGCCCC
    ATTGAGTACC TGTCCTCAGG ATGGAACCTG TTCGACTTCT CCGTCACAGC CTTTGCCTTC
    CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTCT ACTTCATAGT GGTCCTGCGT
    CCCCTCCAGC TGCTGAGGTT GTTTAAGCTG AAGAAGCGTT ACCGCAACGT GCTGGACACC
    ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTGA CTCTGCTCAT CTTCTACTAC
    TCCTTCGCCA TCGTGGGCAT GGAATTCTTC TATGGAATCC TCTACCCCAA CTGCTGCAAT
    ACAAGCACAG TGGCAGACGC CTACCGCTGG CTGAACCACT CCGTGGGCAA CAGGACCGTC
    GTGGAGGAGG GCTATTACTA TCTCAATAAT TTTGACAACA TCCTGAACAG CTTTGTGACC
    TTGTTCGAGC TCACGGTTGT CAACAACTGG TACATCATCA TGGAAGGTGT CACCTCCCAG
    ACCTCGCACT GGAGCCGCCT CTACTTCATG ACCTTCTACA TCGTGACCAT GGTGGTGATG
    ACCATCATTG TGGCCTTCAT CCTCGAAGCC TTTGTCTTCC GAATGAACTA CAGCCGCAAG
    AACCAGGACT CGGAAGTGGA CGACGGCATC ACCCTGGAGA AGGAGCTCTC CAAAGACGAG
    CTGGTCGCAG TTCTGAAGCT CTACCGGGAG GCAGGGGGAG CCAACTCAGA CATTGCCCAG
    CTGCTCAAGA TCCTCTCCCA GCTGGAGAGA TACGAGCAAA ATACCTTGGT GTTTCTGGGA
    CGGAGATCAA GGACCAAAAG CGACCTGAGC CTAAAGATGT ACCAAGAGGA GATTCAGGAG
    TGGTATGAGG AACATGCCCG CAAGCAGGAG GAACGACAGC TCAGCAGCTG CGTGGTCCCT
    GCCACCCAGC AGCCTCCAGG CAGCCGCCAG CGCTCCCAGA CCATCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002694563.1
    CDS350..2800
    Translation

    Target ORF information:

    RefSeq Version XM_002694517.6
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002694517.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AAATGGAGGC
    AAATATACCG TCCACGACTC CCAGGCCCCC AGTCTGAGCT TAGAGGGTGA GAACTCCCCC
    TCCAGCCCCA CTGAACATGA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG
    GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC TCTGGCGGCC
    TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCGCGGC CTTGCTCCTG
    CTGCTGCTCT CCCTGTGCGA GGCTCCCGCC GTCCCCGCGC TCCGGCTGGG CATTTACGTC
    CACGCAACCC TGGAGCTGTT CGCCCTGGTG GTGGTCGTGT TTGAACTCTG CATGAAGTTG
    CGGTGGCTGG GCCTTCACAC CTTCATCCGG CACAGAAGGA CCATGGTCAA GACCTCCGTG
    CTGGTGGTGC AGTTCGTGGA GGCCATCGTG GTGTTGGTAC GGCAGACGTC ACACATGCGG
    GTGACCCGGG CGCTGCGCTG CATCTTCCTG GTGGACTGTC GGTACTGCGG CGGCGTCCGG
    CGCAACCTGC GTCAGATGTT TCAGTCCCTG CCGCCCTTCA TGGACATCCT CCTGCTTCTC
    CTCTTCTTCA TGATCATTTT CGCCATCCTC GGTTTCTACT TATTCTCCCC TAATCCTTCA
    GACCCCTACT TCAGCACCCT GGAGAACAGC ATCATCAGTC TGTTTGTCCT TCTGACCACC
    GCCAACTTCC CAGACGTGAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC
    TTCATCGTGT ACCTCTCCAT TGAGCTGTAC TTTATCATGA ACCTGCTCCT GGCCGTGGTA
    TTTGACACCT TCAATGACAT CGAGAAACGC AAGTTCAAGT CTTTGCTGCT ACACAAGCGA
    ACTGCCATCC AGCACGCCTA CCGCCTGCTT GTCAGCCAGC GGAGGCCTGC CGGCATCTCC
    TACAGGCAGT TCGAAGGCCT GATGCGTTTC TACAAGCCCC GGATGAGTGC GGGGGAGCGC
    TATCTCACCT TCAAGGCCCT GAATCAGAGC AACACGCCAC TGCTCAGCCT GAAGGACTTT
    CATGATATTT ATGAAGTTGC CGCCTTAAAG TGGAAGGCAA AGAGAAATAG AGAACACTGG
    TTTGATGAGC TTCCCAGGAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAG
    TCCAAGGCCT TCCAGTATTT CATGTATTTG GTGGTAGCGG TCAACGGGGT CTGGATCCTC
    GTGGAGACCT TCATGCTGAA AGGTGGGAAC TTCGTCTCCA GGCACGTGCC GTGGAGTCAC
    CTCGTCTTTC TGACTATCTA TGCGGTAGAG CTGTTCCTGA AAGTTGCCGG CCTGGGCCCC
    ATTGAGTACC TGTCCTCAGG ATGGAACCTG TTCGACTTCT CCGTCACAGC CTTTGCCTTC
    CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTCT ACTTCATAGT GGTCCTGCGT
    CCCCTCCAGC TGCTGAGGTT GTTTAAGCTG AAGAAGCGTT ACCGCAACGT GCTGGACACC
    ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTGA CTCTGCTCAT CTTCTACTAC
    TCCTTCGCCA TCGTGGGCAT GGAATTCTTC TATGGAATCC TCTACCCCAA CTGCTGCAAT
    ACAAGCACAG TGGCAGACGC CTACCGCTGG CTGAACCACT CCGTGGGCAA CAGGACCGTC
    GTGGAGGAGG GCTATTACTA TCTCAATAAT TTTGACAACA TCCTGAACAG CTTTGTGACC
    TTGTTCGAGC TCACGGTTGT CAACAACTGG TACATCATCA TGGAAGGTGT CACCTCCCAG
    ACCTCGCACT GGAGCCGCCT CTACTTCATG ACCTTCTACA TCGTGACCAT GGTGGTGATG
    ACCATCATTG TGGCCTTCAT CCTCGAAGCC TTTGTCTTCC GAATGAACTA CAGCCGCAAG
    AACCAGGACT CGGAAGTGGA CGACGGCATC ACCCTGGAGA AGGAGCTCTC CAAAGACGAG
    CTGGTCGCAG TTCTGAAGCT CTACCGGGAG GCAGGGGGAG CCAACTCAGA CATTGCCCAG
    CTGCTCAAGA TCCTCTCCCA GCTGGAGAGA TACGAGCAAA ATACCTTGGT GTTTCTGGGA
    CGGAGATCAA GGACCAAAAG CGACCTGAGC CTAAAGATGT ACCAAGAGGA GATTCAGGAG
    TGGTATGAGG AACATGCCCG CAAGCAGGAG GAACGACAGC TCAGCAGCTG CGTGGTCCCT
    GCCACCCAGC AGCCTCCAGG CAGCCGCCAG CGCTCCCAGA CCATCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09065
    Clone ID Related Accession (Same CDS sequence) XM_024977760.1 , XM_588037.9 , XM_002694517.6 , XM_024977761.1 , XM_002694517.5
    Accession Version XM_024977761.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AAATGGAGGC
    AAATATACCG TCCACGACTC CCAGGCCCCC AGTCTGAGCT TAGAGGGTGA GAACTCCCCC
    TCCAGCCCCA CTGAACATGA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG
    GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC TCTGGCGGCC
    TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCGCGGC CTTGCTCCTG
    CTGCTGCTCT CCCTGTGCGA GGCTCCCGCC GTCCCCGCGC TCCGGCTGGG CATTTACGTC
    CACGCAACCC TGGAGCTGTT CGCCCTGGTG GTGGTCGTGT TTGAACTCTG CATGAAGTTG
    CGGTGGCTGG GCCTTCACAC CTTCATCCGG CACAGAAGGA CCATGGTCAA GACCTCCGTG
    CTGGTGGTGC AGTTCGTGGA GGCCATCGTG GTGTTGGTAC GGCAGACGTC ACACATGCGG
    GTGACCCGGG CGCTGCGCTG CATCTTCCTG GTGGACTGTC GGTACTGCGG CGGCGTCCGG
    CGCAACCTGC GTCAGATGTT TCAGTCCCTG CCGCCCTTCA TGGACATCCT CCTGCTTCTC
    CTCTTCTTCA TGATCATTTT CGCCATCCTC GGTTTCTACT TATTCTCCCC TAATCCTTCA
    GACCCCTACT TCAGCACCCT GGAGAACAGC ATCATCAGTC TGTTTGTCCT TCTGACCACC
    GCCAACTTCC CAGACGTGAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC
    TTCATCGTGT ACCTCTCCAT TGAGCTGTAC TTTATCATGA ACCTGCTCCT GGCCGTGGTA
    TTTGACACCT TCAATGACAT CGAGAAACGC AAGTTCAAGT CTTTGCTGCT ACACAAGCGA
    ACTGCCATCC AGCACGCCTA CCGCCTGCTT GTCAGCCAGC GGAGGCCTGC CGGCATCTCC
    TACAGGCAGT TCGAAGGCCT GATGCGTTTC TACAAGCCCC GGATGAGTGC GGGGGAGCGC
    TATCTCACCT TCAAGGCCCT GAATCAGAGC AACACGCCAC TGCTCAGCCT GAAGGACTTT
    CATGATATTT ATGAAGTTGC CGCCTTAAAG TGGAAGGCAA AGAGAAATAG AGAACACTGG
    TTTGATGAGC TTCCCAGGAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAG
    TCCAAGGCCT TCCAGTATTT CATGTATTTG GTGGTAGCGG TCAACGGGGT CTGGATCCTC
    GTGGAGACCT TCATGCTGAA AGGTGGGAAC TTCGTCTCCA GGCACGTGCC GTGGAGTCAC
    CTCGTCTTTC TGACTATCTA TGCGGTAGAG CTGTTCCTGA AAGTTGCCGG CCTGGGCCCC
    ATTGAGTACC TGTCCTCAGG ATGGAACCTG TTCGACTTCT CCGTCACAGC CTTTGCCTTC
    CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTCT ACTTCATAGT GGTCCTGCGT
    CCCCTCCAGC TGCTGAGGTT GTTTAAGCTG AAGAAGCGTT ACCGCAACGT GCTGGACACC
    ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTGA CTCTGCTCAT CTTCTACTAC
    TCCTTCGCCA TCGTGGGCAT GGAATTCTTC TATGGAATCC TCTACCCCAA CTGCTGCAAT
    ACAAGCACAG TGGCAGACGC CTACCGCTGG CTGAACCACT CCGTGGGCAA CAGGACCGTC
    GTGGAGGAGG GCTATTACTA TCTCAATAAT TTTGACAACA TCCTGAACAG CTTTGTGACC
    TTGTTCGAGC TCACGGTTGT CAACAACTGG TACATCATCA TGGAAGGTGT CACCTCCCAG
    ACCTCGCACT GGAGCCGCCT CTACTTCATG ACCTTCTACA TCGTGACCAT GGTGGTGATG
    ACCATCATTG TGGCCTTCAT CCTCGAAGCC TTTGTCTTCC GAATGAACTA CAGCCGCAAG
    AACCAGGACT CGGAAGTGGA CGACGGCATC ACCCTGGAGA AGGAGCTCTC CAAAGACGAG
    CTGGTCGCAG TTCTGAAGCT CTACCGGGAG GCAGGGGGAG CCAACTCAGA CATTGCCCAG
    CTGCTCAAGA TCCTCTCCCA GCTGGAGAGA TACGAGCAAA ATACCTTGGT GTTTCTGGGA
    CGGAGATCAA GGACCAAAAG CGACCTGAGC CTAAAGATGT ACCAAGAGGA GATTCAGGAG
    TGGTATGAGG AACATGCCCG CAAGCAGGAG GAACGACAGC TCAGCAGCTG CGTGGTCCCT
    GCCACCCAGC AGCCTCCAGG CAGCCGCCAG CGCTCCCAGA CCATCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833529.1
    CDS307..2757
    Translation

    Target ORF information:

    RefSeq Version XM_024977761.1
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977761.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AAATGGAGGC
    AAATATACCG TCCACGACTC CCAGGCCCCC AGTCTGAGCT TAGAGGGTGA GAACTCCCCC
    TCCAGCCCCA CTGAACATGA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG
    GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC TCTGGCGGCC
    TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCGCGGC CTTGCTCCTG
    CTGCTGCTCT CCCTGTGCGA GGCTCCCGCC GTCCCCGCGC TCCGGCTGGG CATTTACGTC
    CACGCAACCC TGGAGCTGTT CGCCCTGGTG GTGGTCGTGT TTGAACTCTG CATGAAGTTG
    CGGTGGCTGG GCCTTCACAC CTTCATCCGG CACAGAAGGA CCATGGTCAA GACCTCCGTG
    CTGGTGGTGC AGTTCGTGGA GGCCATCGTG GTGTTGGTAC GGCAGACGTC ACACATGCGG
    GTGACCCGGG CGCTGCGCTG CATCTTCCTG GTGGACTGTC GGTACTGCGG CGGCGTCCGG
    CGCAACCTGC GTCAGATGTT TCAGTCCCTG CCGCCCTTCA TGGACATCCT CCTGCTTCTC
    CTCTTCTTCA TGATCATTTT CGCCATCCTC GGTTTCTACT TATTCTCCCC TAATCCTTCA
    GACCCCTACT TCAGCACCCT GGAGAACAGC ATCATCAGTC TGTTTGTCCT TCTGACCACC
    GCCAACTTCC CAGACGTGAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC
    TTCATCGTGT ACCTCTCCAT TGAGCTGTAC TTTATCATGA ACCTGCTCCT GGCCGTGGTA
    TTTGACACCT TCAATGACAT CGAGAAACGC AAGTTCAAGT CTTTGCTGCT ACACAAGCGA
    ACTGCCATCC AGCACGCCTA CCGCCTGCTT GTCAGCCAGC GGAGGCCTGC CGGCATCTCC
    TACAGGCAGT TCGAAGGCCT GATGCGTTTC TACAAGCCCC GGATGAGTGC GGGGGAGCGC
    TATCTCACCT TCAAGGCCCT GAATCAGAGC AACACGCCAC TGCTCAGCCT GAAGGACTTT
    CATGATATTT ATGAAGTTGC CGCCTTAAAG TGGAAGGCAA AGAGAAATAG AGAACACTGG
    TTTGATGAGC TTCCCAGGAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAG
    TCCAAGGCCT TCCAGTATTT CATGTATTTG GTGGTAGCGG TCAACGGGGT CTGGATCCTC
    GTGGAGACCT TCATGCTGAA AGGTGGGAAC TTCGTCTCCA GGCACGTGCC GTGGAGTCAC
    CTCGTCTTTC TGACTATCTA TGCGGTAGAG CTGTTCCTGA AAGTTGCCGG CCTGGGCCCC
    ATTGAGTACC TGTCCTCAGG ATGGAACCTG TTCGACTTCT CCGTCACAGC CTTTGCCTTC
    CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTCT ACTTCATAGT GGTCCTGCGT
    CCCCTCCAGC TGCTGAGGTT GTTTAAGCTG AAGAAGCGTT ACCGCAACGT GCTGGACACC
    ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTGA CTCTGCTCAT CTTCTACTAC
    TCCTTCGCCA TCGTGGGCAT GGAATTCTTC TATGGAATCC TCTACCCCAA CTGCTGCAAT
    ACAAGCACAG TGGCAGACGC CTACCGCTGG CTGAACCACT CCGTGGGCAA CAGGACCGTC
    GTGGAGGAGG GCTATTACTA TCTCAATAAT TTTGACAACA TCCTGAACAG CTTTGTGACC
    TTGTTCGAGC TCACGGTTGT CAACAACTGG TACATCATCA TGGAAGGTGT CACCTCCCAG
    ACCTCGCACT GGAGCCGCCT CTACTTCATG ACCTTCTACA TCGTGACCAT GGTGGTGATG
    ACCATCATTG TGGCCTTCAT CCTCGAAGCC TTTGTCTTCC GAATGAACTA CAGCCGCAAG
    AACCAGGACT CGGAAGTGGA CGACGGCATC ACCCTGGAGA AGGAGCTCTC CAAAGACGAG
    CTGGTCGCAG TTCTGAAGCT CTACCGGGAG GCAGGGGGAG CCAACTCAGA CATTGCCCAG
    CTGCTCAAGA TCCTCTCCCA GCTGGAGAGA TACGAGCAAA ATACCTTGGT GTTTCTGGGA
    CGGAGATCAA GGACCAAAAG CGACCTGAGC CTAAAGATGT ACCAAGAGGA GATTCAGGAG
    TGGTATGAGG AACATGCCCG CAAGCAGGAG GAACGACAGC TCAGCAGCTG CGTGGTCCCT
    GCCACCCAGC AGCCTCCAGG CAGCCGCCAG CGCTCCCAGA CCATCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09065
    Clone ID Related Accession (Same CDS sequence) XM_024977760.1 , XM_588037.9 , XM_002694517.6 , XM_024977761.1 , XM_002694517.5
    Accession Version XM_002694517.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002694517.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AAATGGAGGC
    AAATATACCG TCCACGACTC CCAGGCCCCC AGTCTGAGCT TAGAGGGTGA GAACTCCCCC
    TCCAGCCCCA CTGAACATGA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG
    GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC TCTGGCGGCC
    TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCGCGGC CTTGCTCCTG
    CTGCTGCTCT CCCTGTGCGA GGCTCCCGCC GTCCCCGCGC TCCGGCTGGG CATTTACGTC
    CACGCAACCC TGGAGCTGTT CGCCCTGGTG GTGGTCGTGT TTGAACTCTG CATGAAGTTG
    CGGTGGCTGG GCCTTCACAC CTTCATCCGG CACAGAAGGA CCATGGTCAA GACCTCCGTG
    CTGGTGGTGC AGTTCGTGGA GGCCATCGTG GTGTTGGTAC GGCAGACGTC ACACATGCGG
    GTGACCCGGG CGCTGCGCTG CATCTTCCTG GTGGACTGTC GGTACTGCGG CGGCGTCCGG
    CGCAACCTGC GTCAGATGTT TCAGTCCCTG CCGCCCTTCA TGGACATCCT CCTGCTTCTC
    CTCTTCTTCA TGATCATTTT CGCCATCCTC GGTTTCTACT TATTCTCCCC TAATCCTTCA
    GACCCCTACT TCAGCACCCT GGAGAACAGC ATCATCAGTC TGTTTGTCCT TCTGACCACC
    GCCAACTTCC CAGACGTGAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC
    TTCATCGTGT ACCTCTCCAT TGAGCTGTAC TTTATCATGA ACCTGCTCCT GGCCGTGGTA
    TTTGACACCT TCAATGACAT CGAGAAACGC AAGTTCAAGT CTTTGCTGCT ACACAAGCGA
    ACTGCCATCC AGCACGCCTA CCGCCTGCTT GTCAGCCAGC GGAGGCCTGC CGGCATCTCC
    TACAGGCAGT TCGAAGGCCT GATGCGTTTC TACAAGCCCC GGATGAGTGC GGGGGAGCGC
    TATCTCACCT TCAAGGCCCT GAATCAGAGC AACACGCCAC TGCTCAGCCT GAAGGACTTT
    CATGATATTT ATGAAGTTGC CGCCTTAAAG TGGAAGGCAA AGAGAAATAG AGAACACTGG
    TTTGATGAGC TTCCCAGGAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAG
    TCCAAGGCCT TCCAGTATTT CATGTATTTG GTGGTAGCGG TCAACGGGGT CTGGATCCTC
    GTGGAGACCT TCATGCTGAA AGGTGGGAAC TTCGTCTCCA GGCACGTGCC GTGGAGTCAC
    CTCGTCTTTC TGACTATCTA TGCGGTAGAG CTGTTCCTGA AAGTTGCCGG CCTGGGCCCC
    ATTGAGTACC TGTCCTCAGG ATGGAACCTG TTCGACTTCT CCGTCACAGC CTTTGCCTTC
    CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTCT ACTTCATAGT GGTCCTGCGT
    CCCCTCCAGC TGCTGAGGTT GTTTAAGCTG AAGAAGCGTT ACCGCAACGT GCTGGACACC
    ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTGA CTCTGCTCAT CTTCTACTAC
    TCCTTCGCCA TCGTGGGCAT GGAATTCTTC TATGGAATCC TCTACCCCAA CTGCTGCAAT
    ACAAGCACAG TGGCAGACGC CTACCGCTGG CTGAACCACT CCGTGGGCAA CAGGACCGTC
    GTGGAGGAGG GCTATTACTA TCTCAATAAT TTTGACAACA TCCTGAACAG CTTTGTGACC
    TTGTTCGAGC TCACGGTTGT CAACAACTGG TACATCATCA TGGAAGGTGT CACCTCCCAG
    ACCTCGCACT GGAGCCGCCT CTACTTCATG ACCTTCTACA TCGTGACCAT GGTGGTGATG
    ACCATCATTG TGGCCTTCAT CCTCGAAGCC TTTGTCTTCC GAATGAACTA CAGCCGCAAG
    AACCAGGACT CGGAAGTGGA CGACGGCATC ACCCTGGAGA AGGAGCTCTC CAAAGACGAG
    CTGGTCGCAG TTCTGAAGCT CTACCGGGAG GCAGGGGGAG CCAACTCAGA CATTGCCCAG
    CTGCTCAAGA TCCTCTCCCA GCTGGAGAGA TACGAGCAAA ATACCTTGGT GTTTCTGGGA
    CGGAGATCAA GGACCAAAAG CGACCTGAGC CTAAAGATGT ACCAAGAGGA GATTCAGGAG
    TGGTATGAGG AACATGCCCG CAAGCAGGAG GAACGACAGC TCAGCAGCTG CGTGGTCCCT
    GCCACCCAGC AGCCTCCAGG CAGCCGCCAG CGCTCCCAGA CCATCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002694563.1
    CDS342..2792
    Translation

    Target ORF information:

    RefSeq Version XM_002694517.5
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002694517.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AAATGGAGGC
    AAATATACCG TCCACGACTC CCAGGCCCCC AGTCTGAGCT TAGAGGGTGA GAACTCCCCC
    TCCAGCCCCA CTGAACATGA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG
    GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC TCTGGCGGCC
    TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCGCGGC CTTGCTCCTG
    CTGCTGCTCT CCCTGTGCGA GGCTCCCGCC GTCCCCGCGC TCCGGCTGGG CATTTACGTC
    CACGCAACCC TGGAGCTGTT CGCCCTGGTG GTGGTCGTGT TTGAACTCTG CATGAAGTTG
    CGGTGGCTGG GCCTTCACAC CTTCATCCGG CACAGAAGGA CCATGGTCAA GACCTCCGTG
    CTGGTGGTGC AGTTCGTGGA GGCCATCGTG GTGTTGGTAC GGCAGACGTC ACACATGCGG
    GTGACCCGGG CGCTGCGCTG CATCTTCCTG GTGGACTGTC GGTACTGCGG CGGCGTCCGG
    CGCAACCTGC GTCAGATGTT TCAGTCCCTG CCGCCCTTCA TGGACATCCT CCTGCTTCTC
    CTCTTCTTCA TGATCATTTT CGCCATCCTC GGTTTCTACT TATTCTCCCC TAATCCTTCA
    GACCCCTACT TCAGCACCCT GGAGAACAGC ATCATCAGTC TGTTTGTCCT TCTGACCACC
    GCCAACTTCC CAGACGTGAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC
    TTCATCGTGT ACCTCTCCAT TGAGCTGTAC TTTATCATGA ACCTGCTCCT GGCCGTGGTA
    TTTGACACCT TCAATGACAT CGAGAAACGC AAGTTCAAGT CTTTGCTGCT ACACAAGCGA
    ACTGCCATCC AGCACGCCTA CCGCCTGCTT GTCAGCCAGC GGAGGCCTGC CGGCATCTCC
    TACAGGCAGT TCGAAGGCCT GATGCGTTTC TACAAGCCCC GGATGAGTGC GGGGGAGCGC
    TATCTCACCT TCAAGGCCCT GAATCAGAGC AACACGCCAC TGCTCAGCCT GAAGGACTTT
    CATGATATTT ATGAAGTTGC CGCCTTAAAG TGGAAGGCAA AGAGAAATAG AGAACACTGG
    TTTGATGAGC TTCCCAGGAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAG
    TCCAAGGCCT TCCAGTATTT CATGTATTTG GTGGTAGCGG TCAACGGGGT CTGGATCCTC
    GTGGAGACCT TCATGCTGAA AGGTGGGAAC TTCGTCTCCA GGCACGTGCC GTGGAGTCAC
    CTCGTCTTTC TGACTATCTA TGCGGTAGAG CTGTTCCTGA AAGTTGCCGG CCTGGGCCCC
    ATTGAGTACC TGTCCTCAGG ATGGAACCTG TTCGACTTCT CCGTCACAGC CTTTGCCTTC
    CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTCT ACTTCATAGT GGTCCTGCGT
    CCCCTCCAGC TGCTGAGGTT GTTTAAGCTG AAGAAGCGTT ACCGCAACGT GCTGGACACC
    ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTGA CTCTGCTCAT CTTCTACTAC
    TCCTTCGCCA TCGTGGGCAT GGAATTCTTC TATGGAATCC TCTACCCCAA CTGCTGCAAT
    ACAAGCACAG TGGCAGACGC CTACCGCTGG CTGAACCACT CCGTGGGCAA CAGGACCGTC
    GTGGAGGAGG GCTATTACTA TCTCAATAAT TTTGACAACA TCCTGAACAG CTTTGTGACC
    TTGTTCGAGC TCACGGTTGT CAACAACTGG TACATCATCA TGGAAGGTGT CACCTCCCAG
    ACCTCGCACT GGAGCCGCCT CTACTTCATG ACCTTCTACA TCGTGACCAT GGTGGTGATG
    ACCATCATTG TGGCCTTCAT CCTCGAAGCC TTTGTCTTCC GAATGAACTA CAGCCGCAAG
    AACCAGGACT CGGAAGTGGA CGACGGCATC ACCCTGGAGA AGGAGCTCTC CAAAGACGAG
    CTGGTCGCAG TTCTGAAGCT CTACCGGGAG GCAGGGGGAG CCAACTCAGA CATTGCCCAG
    CTGCTCAAGA TCCTCTCCCA GCTGGAGAGA TACGAGCAAA ATACCTTGGT GTTTCTGGGA
    CGGAGATCAA GGACCAAAAG CGACCTGAGC CTAAAGATGT ACCAAGAGGA GATTCAGGAG
    TGGTATGAGG AACATGCCCG CAAGCAGGAG GAACGACAGC TCAGCAGCTG CGTGGTCCCT
    GCCACCCAGC AGCCTCCAGG CAGCCGCCAG CGCTCCCAGA CCATCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47815
    Clone ID Related Accession (Same CDS sequence) XM_010823418.2 , XM_010814054.3 , XM_024977762.1 , XM_010814054.2
    Accession Version XM_010823418.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823418.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC 
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821720.1
    CDS119..2362
    Translation

    Target ORF information:

    RefSeq Version XM_010823418.2
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823418.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC 
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47815
    Clone ID Related Accession (Same CDS sequence) XM_010823418.2 , XM_010814054.3 , XM_024977762.1 , XM_010814054.2
    Accession Version XM_010814054.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814054.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC 
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812356.1
    CDS218..2461
    Translation

    Target ORF information:

    RefSeq Version XM_010814054.3
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814054.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC 
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47815
    Clone ID Related Accession (Same CDS sequence) XM_010823418.2 , XM_010814054.3 , XM_024977762.1 , XM_010814054.2
    Accession Version XM_024977762.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC 
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833530.1
    CDS763..3006
    Translation

    Target ORF information:

    RefSeq Version XM_024977762.1
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977762.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC 
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47815
    Clone ID Related Accession (Same CDS sequence) XM_010823418.2 , XM_010814054.3 , XM_024977762.1 , XM_010814054.2
    Accession Version XM_010814054.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814054.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC 
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812356.1
    CDS119..2362
    Translation

    Target ORF information:

    RefSeq Version XM_010814054.2
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814054.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATTATC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAATAACGA CAAGTTCTTC 
    ACCCACCCCA AGAACGCCAA AGCTCTGGCG GCCTACCTCT TCGCACACAA CCACCTCTTC
    TACCTGATGG AGCTGTCCGC GGCCTTGCTC CTGCTGCTGC TCTCCCTGTG CGAGGCTCCC
    GCCGTCCCCG CGCTCCGGCT GGGCATTTAC GTCCACGCAA CCCTGGAGCT GTTCGCCCTG
    GTGGTGGTCG TGTTTGAACT CTGCATGAAG TTGCGGTGGC TGGGCCTTCA CACCTTCATC
    CGGCACAGAA GGACCATGGT CAAGACCTCC GTGCTGGTGG TGCAGTTCGT GGAGGCCATC
    GTGGTGTTGG TACGGCAGAC GTCACACATG CGGGTGACCC GGGCGCTGCG CTGCATCTTC
    CTGGTGGACT GTCGGTACTG CGGCGGCGTC CGGCGCAACC TGCGTCAGAT GTTTCAGTCC
    CTGCCGCCCT TCATGGACAT CCTCCTGCTT CTCCTCTTCT TCATGATCAT TTTCGCCATC
    CTCGGTTTCT ACTTATTCTC CCCTAATCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC
    AGCATCATCA GTCTGTTTGT CCTTCTGACC ACCGCCAACT TCCCAGACGT GATGATGCCC
    TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATTGAGCTG
    TACTTTATCA TGAACCTGCT CCTGGCCGTG GTATTTGACA CCTTCAATGA CATCGAGAAA
    CGCAAGTTCA AGTCTTTGCT GCTACACAAG CGAACTGCCA TCCAGCACGC CTACCGCCTG
    CTTGTCAGCC AGCGGAGGCC TGCCGGCATC TCCTACAGGC AGTTCGAAGG CCTGATGCGT
    TTCTACAAGC CCCGGATGAG TGCGGGGGAG CGCTATCTCA CCTTCAAGGC CCTGAATCAG
    AGCAACACGC CACTGCTCAG CCTGAAGGAC TTTCATGATA TTTATGAAGT TGCCGCCTTA
    AAGTGGAAGG CAAAGAGAAA TAGAGAACAC TGGTTTGATG AGCTTCCCAG GACAGCATTC
    CTCATCTTCA AAGGAATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAT
    TTGGTGGTAG CGGTCAACGG GGTCTGGATC CTCGTGGAGA CCTTCATGCT GAAAGGTGGG
    AACTTCGTCT CCAGGCACGT GCCGTGGAGT CACCTCGTCT TTCTGACTAT CTATGCGGTA
    GAGCTGTTCC TGAAAGTTGC CGGCCTGGGC CCCATTGAGT ACCTGTCCTC AGGATGGAAC
    CTGTTCGACT TCTCCGTCAC AGCCTTTGCC TTCCTGGGAC TGCTGGCTCT GGCCTTCAAC
    ATGGAGCCCT TCTACTTCAT AGTGGTCCTG CGTCCCCTCC AGCTGCTGAG GTTGTTTAAG
    CTGAAGAAGC GTTACCGCAA CGTGCTGGAC ACCATGTTTG AGCTGCTGCC CCGGATGGCC
    AGCCTGGGCC TGACTCTGCT CATCTTCTAC TACTCCTTCG CCATCGTGGG CATGGAATTC
    TTCTATGGAA TCCTCTACCC CAACTGCTGC AATACAAGCA CAGTGGCAGA CGCCTACCGC
    TGGCTGAACC ACTCCGTGGG CAACAGGACC GTCGTGGAGG AGGGCTATTA CTATCTCAAT
    AATTTTGACA ACATCCTGAA CAGCTTTGTG ACCTTGTTCG AGCTCACGGT TGTCAACAAC
    TGGTACATCA TCATGGAAGG TGTCACCTCC CAGACCTCGC ACTGGAGCCG CCTCTACTTC
    ATGACCTTCT ACATCGTGAC CATGGTGGTG ATGACCATCA TTGTGGCCTT CATCCTCGAA
    GCCTTTGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT GGACGACGGC
    ATCACCCTGG AGAAGGAGCT CTCCAAAGAC GAGCTGGTCG CAGTTCTGAA GCTCTACCGG
    GAGGCAGGGG GAGCCAACTC AGACATTGCC CAGCTGCTCA AGATCCTCTC CCAGCTGGAG
    AGATACGAGC AAAATACCTT GGTGTTTCTG GGACGGAGAT CAAGGACCAA AAGCGACCTG
    AGCCTAAAGA TGTACCAAGA GGAGATTCAG GAGTGGTATG AGGAACATGC CCGCAAGCAG
    GAGGAACGAC AGCTCAGCAG CTGCGTGGTC CCTGCCACCC AGCAGCCTCC AGGCAGCCGC
    CAGCGCTCCC AGACCATCAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09063
    Clone ID Related Accession (Same CDS sequence) XM_024977758.1 , XM_005218006.4 , XM_024977757.1
    Accession Version XM_024977758.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACGGAAGGT
    GAATCCTCAA CCGCTGGACC GCCAGGGACG TCCCCATCTG GGCCTTTCTT ATCTTGTGCT
    TGGATCTTGC ACGGACTTTC TTCTCAAGGC CCGCCTCCCA CCTGTCTGAA TGGTCCATCT
    AAAACACAAA TCCGAGCAGC TGCCTCCCAG GAAAGGCGGA AATGCAGAGG CGAGGGCCAG
    CCATCATCTT CCTGGAGGAG GCAGCAGAGG GCCAGCTCCC TGACAGCCCC ATGGAGAGGT
    CATCCCGTCT CCTCCAGCTG GGCCCTTGTG GTATGCCTTG ACCTGGCCGT GGATGGAGGC
    AAATATACCG TCCACGACTC CCAGGCCCCC AGTCTGAGCT TAGAGGGTGA GAACTCCCCC
    TCCAGCCCCA CTGAACATGA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG
    GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC TCTGGCGGCC
    TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCGCGGC CTTGCTCCTG
    CTGCTGCTCT CCCTGTGCGA GGCTCCCGCC GTCCCCGCGC TCCGGCTGGG CATTTACGTC
    CACGCAACCC TGGAGCTGTT CGCCCTGGTG GTGGTCGTGT TTGAACTCTG CATGAAGTTG
    CGGTGGCTGG GCCTTCACAC CTTCATCCGG CACAGAAGGA CCATGGTCAA GACCTCCGTG
    CTGGTGGTGC AGTTCGTGGA GGCCATCGTG GTGTTGGTAC GGCAGACGTC ACACATGCGG
    GTGACCCGGG CGCTGCGCTG CATCTTCCTG GTGGACTGTC GGTACTGCGG CGGCGTCCGG
    CGCAACCTGC GTCAGATGTT TCAGTCCCTG CCGCCCTTCA TGGACATCCT CCTGCTTCTC
    CTCTTCTTCA TGATCATTTT CGCCATCCTC GGTTTCTACT TATTCTCCCC TAATCCTTCA
    GACCCCTACT TCAGCACCCT GGAGAACAGC ATCATCAGTC TGTTTGTCCT TCTGACCACC
    GCCAACTTCC CAGACGTGAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC
    TTCATCGTGT ACCTCTCCAT TGAGCTGTAC TTTATCATGA ACCTGCTCCT GGCCGTGGTA
    TTTGACACCT TCAATGACAT CGAGAAACGC AAGTTCAAGT CTTTGCTGCT ACACAAGCGA
    ACTGCCATCC AGCACGCCTA CCGCCTGCTT GTCAGCCAGC GGAGGCCTGC CGGCATCTCC
    TACAGGCAGT TCGAAGGCCT GATGCGTTTC TACAAGCCCC GGATGAGTGC GGGGGAGCGC
    TATCTCACCT TCAAGGCCCT GAATCAGAGC AACACGCCAC TGCTCAGCCT GAAGGACTTT
    CATGATATTT ATGAAGTTGC CGCCTTAAAG TGGAAGGCAA AGAGAAATAG AGAACACTGG
    TTTGATGAGC TTCCCAGGAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAG
    TCCAAGGCCT TCCAGTATTT CATGTATTTG GTGGTAGCGG TCAACGGGGT CTGGATCCTC
    GTGGAGACCT TCATGCTGAA AGGTGGGAAC TTCGTCTCCA GGCACGTGCC GTGGAGTCAC
    CTCGTCTTTC TGACTATCTA TGCGGTAGAG CTGTTCCTGA AAGTTGCCGG CCTGGGCCCC
    ATTGAGTACC TGTCCTCAGG ATGGAACCTG TTCGACTTCT CCGTCACAGC CTTTGCCTTC
    CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTCT ACTTCATAGT GGTCCTGCGT
    CCCCTCCAGC TGCTGAGGTT GTTTAAGCTG AAGAAGCGTT ACCGCAACGT GCTGGACACC
    ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTGA CTCTGCTCAT CTTCTACTAC
    TCCTTCGCCA TCGTGGGCAT GGAATTCTTC TATGGAATCC TCTACCCCAA CTGCTGCAAT
    ACAAGCACAG TGGCAGACGC CTACCGCTGG CTGAACCACT CCGTGGGCAA CAGGACCGTC
    GTGGAGGAGG GCTATTACTA TCTCAATAAT TTTGACAACA TCCTGAACAG CTTTGTGACC
    TTGTTCGAGC TCACGGTTGT CAACAACTGG TACATCATCA TGGAAGGTGT CACCTCCCAG
    ACCTCGCACT GGAGCCGCCT CTACTTCATG ACCTTCTACA TCGTGACCAT GGTGGTGATG
    ACCATCATTG TGGCCTTCAT CCTCGAAGCC TTTGTCTTCC GAATGAACTA CAGCCGCAAG
    AACCAGGACT CGGAAGTGGA CGACGGCATC ACCCTGGAGA AGGAGCTCTC CAAAGACGAG
    CTGGTCGCAG TTCTGAAGCT CTACCGGGAG GCAGGGGGAG CCAACTCAGA CATTGCCCAG
    CTGCTCAAGA TCCTCTCCCA GCTGGAGAGA TACGAGCAAA ATACCTTGGT GTTTCTGGGA
    CGGAGATCAA GGACCAAAAG CGACCTGAGC CTAAAGATGT ACCAAGAGGA GATTCAGGAG
    TGGTATGAGG AACATGCCCG CAAGCAGGAG GAACGACAGC TCAGCAGCTG CGTGGTCCCT
    GCCACCCAGC AGCCTCCAGG CAGCCGCCAG CGCTCCCAGA CCATCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833526.1
    CDS349..3099
    Translation

    Target ORF information:

    RefSeq Version XM_024977758.1
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977758.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACGGAAGGT
    GAATCCTCAA CCGCTGGACC GCCAGGGACG TCCCCATCTG GGCCTTTCTT ATCTTGTGCT
    TGGATCTTGC ACGGACTTTC TTCTCAAGGC CCGCCTCCCA CCTGTCTGAA TGGTCCATCT
    AAAACACAAA TCCGAGCAGC TGCCTCCCAG GAAAGGCGGA AATGCAGAGG CGAGGGCCAG
    CCATCATCTT CCTGGAGGAG GCAGCAGAGG GCCAGCTCCC TGACAGCCCC ATGGAGAGGT
    CATCCCGTCT CCTCCAGCTG GGCCCTTGTG GTATGCCTTG ACCTGGCCGT GGATGGAGGC
    AAATATACCG TCCACGACTC CCAGGCCCCC AGTCTGAGCT TAGAGGGTGA GAACTCCCCC
    TCCAGCCCCA CTGAACATGA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG
    GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC TCTGGCGGCC
    TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCGCGGC CTTGCTCCTG
    CTGCTGCTCT CCCTGTGCGA GGCTCCCGCC GTCCCCGCGC TCCGGCTGGG CATTTACGTC
    CACGCAACCC TGGAGCTGTT CGCCCTGGTG GTGGTCGTGT TTGAACTCTG CATGAAGTTG
    CGGTGGCTGG GCCTTCACAC CTTCATCCGG CACAGAAGGA CCATGGTCAA GACCTCCGTG
    CTGGTGGTGC AGTTCGTGGA GGCCATCGTG GTGTTGGTAC GGCAGACGTC ACACATGCGG
    GTGACCCGGG CGCTGCGCTG CATCTTCCTG GTGGACTGTC GGTACTGCGG CGGCGTCCGG
    CGCAACCTGC GTCAGATGTT TCAGTCCCTG CCGCCCTTCA TGGACATCCT CCTGCTTCTC
    CTCTTCTTCA TGATCATTTT CGCCATCCTC GGTTTCTACT TATTCTCCCC TAATCCTTCA
    GACCCCTACT TCAGCACCCT GGAGAACAGC ATCATCAGTC TGTTTGTCCT TCTGACCACC
    GCCAACTTCC CAGACGTGAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC
    TTCATCGTGT ACCTCTCCAT TGAGCTGTAC TTTATCATGA ACCTGCTCCT GGCCGTGGTA
    TTTGACACCT TCAATGACAT CGAGAAACGC AAGTTCAAGT CTTTGCTGCT ACACAAGCGA
    ACTGCCATCC AGCACGCCTA CCGCCTGCTT GTCAGCCAGC GGAGGCCTGC CGGCATCTCC
    TACAGGCAGT TCGAAGGCCT GATGCGTTTC TACAAGCCCC GGATGAGTGC GGGGGAGCGC
    TATCTCACCT TCAAGGCCCT GAATCAGAGC AACACGCCAC TGCTCAGCCT GAAGGACTTT
    CATGATATTT ATGAAGTTGC CGCCTTAAAG TGGAAGGCAA AGAGAAATAG AGAACACTGG
    TTTGATGAGC TTCCCAGGAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAG
    TCCAAGGCCT TCCAGTATTT CATGTATTTG GTGGTAGCGG TCAACGGGGT CTGGATCCTC
    GTGGAGACCT TCATGCTGAA AGGTGGGAAC TTCGTCTCCA GGCACGTGCC GTGGAGTCAC
    CTCGTCTTTC TGACTATCTA TGCGGTAGAG CTGTTCCTGA AAGTTGCCGG CCTGGGCCCC
    ATTGAGTACC TGTCCTCAGG ATGGAACCTG TTCGACTTCT CCGTCACAGC CTTTGCCTTC
    CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTCT ACTTCATAGT GGTCCTGCGT
    CCCCTCCAGC TGCTGAGGTT GTTTAAGCTG AAGAAGCGTT ACCGCAACGT GCTGGACACC
    ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTGA CTCTGCTCAT CTTCTACTAC
    TCCTTCGCCA TCGTGGGCAT GGAATTCTTC TATGGAATCC TCTACCCCAA CTGCTGCAAT
    ACAAGCACAG TGGCAGACGC CTACCGCTGG CTGAACCACT CCGTGGGCAA CAGGACCGTC
    GTGGAGGAGG GCTATTACTA TCTCAATAAT TTTGACAACA TCCTGAACAG CTTTGTGACC
    TTGTTCGAGC TCACGGTTGT CAACAACTGG TACATCATCA TGGAAGGTGT CACCTCCCAG
    ACCTCGCACT GGAGCCGCCT CTACTTCATG ACCTTCTACA TCGTGACCAT GGTGGTGATG
    ACCATCATTG TGGCCTTCAT CCTCGAAGCC TTTGTCTTCC GAATGAACTA CAGCCGCAAG
    AACCAGGACT CGGAAGTGGA CGACGGCATC ACCCTGGAGA AGGAGCTCTC CAAAGACGAG
    CTGGTCGCAG TTCTGAAGCT CTACCGGGAG GCAGGGGGAG CCAACTCAGA CATTGCCCAG
    CTGCTCAAGA TCCTCTCCCA GCTGGAGAGA TACGAGCAAA ATACCTTGGT GTTTCTGGGA
    CGGAGATCAA GGACCAAAAG CGACCTGAGC CTAAAGATGT ACCAAGAGGA GATTCAGGAG
    TGGTATGAGG AACATGCCCG CAAGCAGGAG GAACGACAGC TCAGCAGCTG CGTGGTCCCT
    GCCACCCAGC AGCCTCCAGG CAGCCGCCAG CGCTCCCAGA CCATCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09063
    Clone ID Related Accession (Same CDS sequence) XM_024977758.1 , XM_005218006.4 , XM_024977757.1
    Accession Version XM_005218006.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218006.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACGGAAGGT
    GAATCCTCAA CCGCTGGACC GCCAGGGACG TCCCCATCTG GGCCTTTCTT ATCTTGTGCT
    TGGATCTTGC ACGGACTTTC TTCTCAAGGC CCGCCTCCCA CCTGTCTGAA TGGTCCATCT
    AAAACACAAA TCCGAGCAGC TGCCTCCCAG GAAAGGCGGA AATGCAGAGG CGAGGGCCAG
    CCATCATCTT CCTGGAGGAG GCAGCAGAGG GCCAGCTCCC TGACAGCCCC ATGGAGAGGT
    CATCCCGTCT CCTCCAGCTG GGCCCTTGTG GTATGCCTTG ACCTGGCCGT GGATGGAGGC
    AAATATACCG TCCACGACTC CCAGGCCCCC AGTCTGAGCT TAGAGGGTGA GAACTCCCCC
    TCCAGCCCCA CTGAACATGA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG
    GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC TCTGGCGGCC
    TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCGCGGC CTTGCTCCTG
    CTGCTGCTCT CCCTGTGCGA GGCTCCCGCC GTCCCCGCGC TCCGGCTGGG CATTTACGTC
    CACGCAACCC TGGAGCTGTT CGCCCTGGTG GTGGTCGTGT TTGAACTCTG CATGAAGTTG
    CGGTGGCTGG GCCTTCACAC CTTCATCCGG CACAGAAGGA CCATGGTCAA GACCTCCGTG
    CTGGTGGTGC AGTTCGTGGA GGCCATCGTG GTGTTGGTAC GGCAGACGTC ACACATGCGG
    GTGACCCGGG CGCTGCGCTG CATCTTCCTG GTGGACTGTC GGTACTGCGG CGGCGTCCGG
    CGCAACCTGC GTCAGATGTT TCAGTCCCTG CCGCCCTTCA TGGACATCCT CCTGCTTCTC
    CTCTTCTTCA TGATCATTTT CGCCATCCTC GGTTTCTACT TATTCTCCCC TAATCCTTCA
    GACCCCTACT TCAGCACCCT GGAGAACAGC ATCATCAGTC TGTTTGTCCT TCTGACCACC
    GCCAACTTCC CAGACGTGAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC
    TTCATCGTGT ACCTCTCCAT TGAGCTGTAC TTTATCATGA ACCTGCTCCT GGCCGTGGTA
    TTTGACACCT TCAATGACAT CGAGAAACGC AAGTTCAAGT CTTTGCTGCT ACACAAGCGA
    ACTGCCATCC AGCACGCCTA CCGCCTGCTT GTCAGCCAGC GGAGGCCTGC CGGCATCTCC
    TACAGGCAGT TCGAAGGCCT GATGCGTTTC TACAAGCCCC GGATGAGTGC GGGGGAGCGC
    TATCTCACCT TCAAGGCCCT GAATCAGAGC AACACGCCAC TGCTCAGCCT GAAGGACTTT
    CATGATATTT ATGAAGTTGC CGCCTTAAAG TGGAAGGCAA AGAGAAATAG AGAACACTGG
    TTTGATGAGC TTCCCAGGAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAG
    TCCAAGGCCT TCCAGTATTT CATGTATTTG GTGGTAGCGG TCAACGGGGT CTGGATCCTC
    GTGGAGACCT TCATGCTGAA AGGTGGGAAC TTCGTCTCCA GGCACGTGCC GTGGAGTCAC
    CTCGTCTTTC TGACTATCTA TGCGGTAGAG CTGTTCCTGA AAGTTGCCGG CCTGGGCCCC
    ATTGAGTACC TGTCCTCAGG ATGGAACCTG TTCGACTTCT CCGTCACAGC CTTTGCCTTC
    CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTCT ACTTCATAGT GGTCCTGCGT
    CCCCTCCAGC TGCTGAGGTT GTTTAAGCTG AAGAAGCGTT ACCGCAACGT GCTGGACACC
    ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTGA CTCTGCTCAT CTTCTACTAC
    TCCTTCGCCA TCGTGGGCAT GGAATTCTTC TATGGAATCC TCTACCCCAA CTGCTGCAAT
    ACAAGCACAG TGGCAGACGC CTACCGCTGG CTGAACCACT CCGTGGGCAA CAGGACCGTC
    GTGGAGGAGG GCTATTACTA TCTCAATAAT TTTGACAACA TCCTGAACAG CTTTGTGACC
    TTGTTCGAGC TCACGGTTGT CAACAACTGG TACATCATCA TGGAAGGTGT CACCTCCCAG
    ACCTCGCACT GGAGCCGCCT CTACTTCATG ACCTTCTACA TCGTGACCAT GGTGGTGATG
    ACCATCATTG TGGCCTTCAT CCTCGAAGCC TTTGTCTTCC GAATGAACTA CAGCCGCAAG
    AACCAGGACT CGGAAGTGGA CGACGGCATC ACCCTGGAGA AGGAGCTCTC CAAAGACGAG
    CTGGTCGCAG TTCTGAAGCT CTACCGGGAG GCAGGGGGAG CCAACTCAGA CATTGCCCAG
    CTGCTCAAGA TCCTCTCCCA GCTGGAGAGA TACGAGCAAA ATACCTTGGT GTTTCTGGGA
    CGGAGATCAA GGACCAAAAG CGACCTGAGC CTAAAGATGT ACCAAGAGGA GATTCAGGAG
    TGGTATGAGG AACATGCCCG CAAGCAGGAG GAACGACAGC TCAGCAGCTG CGTGGTCCCT
    GCCACCCAGC AGCCTCCAGG CAGCCGCCAG CGCTCCCAGA CCATCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218063.2
    CDS306..3056
    Translation

    Target ORF information:

    RefSeq Version XM_005218006.4
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218006.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACGGAAGGT
    GAATCCTCAA CCGCTGGACC GCCAGGGACG TCCCCATCTG GGCCTTTCTT ATCTTGTGCT
    TGGATCTTGC ACGGACTTTC TTCTCAAGGC CCGCCTCCCA CCTGTCTGAA TGGTCCATCT
    AAAACACAAA TCCGAGCAGC TGCCTCCCAG GAAAGGCGGA AATGCAGAGG CGAGGGCCAG
    CCATCATCTT CCTGGAGGAG GCAGCAGAGG GCCAGCTCCC TGACAGCCCC ATGGAGAGGT
    CATCCCGTCT CCTCCAGCTG GGCCCTTGTG GTATGCCTTG ACCTGGCCGT GGATGGAGGC
    AAATATACCG TCCACGACTC CCAGGCCCCC AGTCTGAGCT TAGAGGGTGA GAACTCCCCC
    TCCAGCCCCA CTGAACATGA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG
    GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC TCTGGCGGCC
    TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCGCGGC CTTGCTCCTG
    CTGCTGCTCT CCCTGTGCGA GGCTCCCGCC GTCCCCGCGC TCCGGCTGGG CATTTACGTC
    CACGCAACCC TGGAGCTGTT CGCCCTGGTG GTGGTCGTGT TTGAACTCTG CATGAAGTTG
    CGGTGGCTGG GCCTTCACAC CTTCATCCGG CACAGAAGGA CCATGGTCAA GACCTCCGTG
    CTGGTGGTGC AGTTCGTGGA GGCCATCGTG GTGTTGGTAC GGCAGACGTC ACACATGCGG
    GTGACCCGGG CGCTGCGCTG CATCTTCCTG GTGGACTGTC GGTACTGCGG CGGCGTCCGG
    CGCAACCTGC GTCAGATGTT TCAGTCCCTG CCGCCCTTCA TGGACATCCT CCTGCTTCTC
    CTCTTCTTCA TGATCATTTT CGCCATCCTC GGTTTCTACT TATTCTCCCC TAATCCTTCA
    GACCCCTACT TCAGCACCCT GGAGAACAGC ATCATCAGTC TGTTTGTCCT TCTGACCACC
    GCCAACTTCC CAGACGTGAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC
    TTCATCGTGT ACCTCTCCAT TGAGCTGTAC TTTATCATGA ACCTGCTCCT GGCCGTGGTA
    TTTGACACCT TCAATGACAT CGAGAAACGC AAGTTCAAGT CTTTGCTGCT ACACAAGCGA
    ACTGCCATCC AGCACGCCTA CCGCCTGCTT GTCAGCCAGC GGAGGCCTGC CGGCATCTCC
    TACAGGCAGT TCGAAGGCCT GATGCGTTTC TACAAGCCCC GGATGAGTGC GGGGGAGCGC
    TATCTCACCT TCAAGGCCCT GAATCAGAGC AACACGCCAC TGCTCAGCCT GAAGGACTTT
    CATGATATTT ATGAAGTTGC CGCCTTAAAG TGGAAGGCAA AGAGAAATAG AGAACACTGG
    TTTGATGAGC TTCCCAGGAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAG
    TCCAAGGCCT TCCAGTATTT CATGTATTTG GTGGTAGCGG TCAACGGGGT CTGGATCCTC
    GTGGAGACCT TCATGCTGAA AGGTGGGAAC TTCGTCTCCA GGCACGTGCC GTGGAGTCAC
    CTCGTCTTTC TGACTATCTA TGCGGTAGAG CTGTTCCTGA AAGTTGCCGG CCTGGGCCCC
    ATTGAGTACC TGTCCTCAGG ATGGAACCTG TTCGACTTCT CCGTCACAGC CTTTGCCTTC
    CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTCT ACTTCATAGT GGTCCTGCGT
    CCCCTCCAGC TGCTGAGGTT GTTTAAGCTG AAGAAGCGTT ACCGCAACGT GCTGGACACC
    ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTGA CTCTGCTCAT CTTCTACTAC
    TCCTTCGCCA TCGTGGGCAT GGAATTCTTC TATGGAATCC TCTACCCCAA CTGCTGCAAT
    ACAAGCACAG TGGCAGACGC CTACCGCTGG CTGAACCACT CCGTGGGCAA CAGGACCGTC
    GTGGAGGAGG GCTATTACTA TCTCAATAAT TTTGACAACA TCCTGAACAG CTTTGTGACC
    TTGTTCGAGC TCACGGTTGT CAACAACTGG TACATCATCA TGGAAGGTGT CACCTCCCAG
    ACCTCGCACT GGAGCCGCCT CTACTTCATG ACCTTCTACA TCGTGACCAT GGTGGTGATG
    ACCATCATTG TGGCCTTCAT CCTCGAAGCC TTTGTCTTCC GAATGAACTA CAGCCGCAAG
    AACCAGGACT CGGAAGTGGA CGACGGCATC ACCCTGGAGA AGGAGCTCTC CAAAGACGAG
    CTGGTCGCAG TTCTGAAGCT CTACCGGGAG GCAGGGGGAG CCAACTCAGA CATTGCCCAG
    CTGCTCAAGA TCCTCTCCCA GCTGGAGAGA TACGAGCAAA ATACCTTGGT GTTTCTGGGA
    CGGAGATCAA GGACCAAAAG CGACCTGAGC CTAAAGATGT ACCAAGAGGA GATTCAGGAG
    TGGTATGAGG AACATGCCCG CAAGCAGGAG GAACGACAGC TCAGCAGCTG CGTGGTCCCT
    GCCACCCAGC AGCCTCCAGG CAGCCGCCAG CGCTCCCAGA CCATCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09063
    Clone ID Related Accession (Same CDS sequence) XM_024977758.1 , XM_005218006.4 , XM_024977757.1
    Accession Version XM_024977757.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACGGAAGGT
    GAATCCTCAA CCGCTGGACC GCCAGGGACG TCCCCATCTG GGCCTTTCTT ATCTTGTGCT
    TGGATCTTGC ACGGACTTTC TTCTCAAGGC CCGCCTCCCA CCTGTCTGAA TGGTCCATCT
    AAAACACAAA TCCGAGCAGC TGCCTCCCAG GAAAGGCGGA AATGCAGAGG CGAGGGCCAG
    CCATCATCTT CCTGGAGGAG GCAGCAGAGG GCCAGCTCCC TGACAGCCCC ATGGAGAGGT
    CATCCCGTCT CCTCCAGCTG GGCCCTTGTG GTATGCCTTG ACCTGGCCGT GGATGGAGGC
    AAATATACCG TCCACGACTC CCAGGCCCCC AGTCTGAGCT TAGAGGGTGA GAACTCCCCC
    TCCAGCCCCA CTGAACATGA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG
    GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC TCTGGCGGCC
    TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCGCGGC CTTGCTCCTG
    CTGCTGCTCT CCCTGTGCGA GGCTCCCGCC GTCCCCGCGC TCCGGCTGGG CATTTACGTC
    CACGCAACCC TGGAGCTGTT CGCCCTGGTG GTGGTCGTGT TTGAACTCTG CATGAAGTTG
    CGGTGGCTGG GCCTTCACAC CTTCATCCGG CACAGAAGGA CCATGGTCAA GACCTCCGTG
    CTGGTGGTGC AGTTCGTGGA GGCCATCGTG GTGTTGGTAC GGCAGACGTC ACACATGCGG
    GTGACCCGGG CGCTGCGCTG CATCTTCCTG GTGGACTGTC GGTACTGCGG CGGCGTCCGG
    CGCAACCTGC GTCAGATGTT TCAGTCCCTG CCGCCCTTCA TGGACATCCT CCTGCTTCTC
    CTCTTCTTCA TGATCATTTT CGCCATCCTC GGTTTCTACT TATTCTCCCC TAATCCTTCA
    GACCCCTACT TCAGCACCCT GGAGAACAGC ATCATCAGTC TGTTTGTCCT TCTGACCACC
    GCCAACTTCC CAGACGTGAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC
    TTCATCGTGT ACCTCTCCAT TGAGCTGTAC TTTATCATGA ACCTGCTCCT GGCCGTGGTA
    TTTGACACCT TCAATGACAT CGAGAAACGC AAGTTCAAGT CTTTGCTGCT ACACAAGCGA
    ACTGCCATCC AGCACGCCTA CCGCCTGCTT GTCAGCCAGC GGAGGCCTGC CGGCATCTCC
    TACAGGCAGT TCGAAGGCCT GATGCGTTTC TACAAGCCCC GGATGAGTGC GGGGGAGCGC
    TATCTCACCT TCAAGGCCCT GAATCAGAGC AACACGCCAC TGCTCAGCCT GAAGGACTTT
    CATGATATTT ATGAAGTTGC CGCCTTAAAG TGGAAGGCAA AGAGAAATAG AGAACACTGG
    TTTGATGAGC TTCCCAGGAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAG
    TCCAAGGCCT TCCAGTATTT CATGTATTTG GTGGTAGCGG TCAACGGGGT CTGGATCCTC
    GTGGAGACCT TCATGCTGAA AGGTGGGAAC TTCGTCTCCA GGCACGTGCC GTGGAGTCAC
    CTCGTCTTTC TGACTATCTA TGCGGTAGAG CTGTTCCTGA AAGTTGCCGG CCTGGGCCCC
    ATTGAGTACC TGTCCTCAGG ATGGAACCTG TTCGACTTCT CCGTCACAGC CTTTGCCTTC
    CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTCT ACTTCATAGT GGTCCTGCGT
    CCCCTCCAGC TGCTGAGGTT GTTTAAGCTG AAGAAGCGTT ACCGCAACGT GCTGGACACC
    ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTGA CTCTGCTCAT CTTCTACTAC
    TCCTTCGCCA TCGTGGGCAT GGAATTCTTC TATGGAATCC TCTACCCCAA CTGCTGCAAT
    ACAAGCACAG TGGCAGACGC CTACCGCTGG CTGAACCACT CCGTGGGCAA CAGGACCGTC
    GTGGAGGAGG GCTATTACTA TCTCAATAAT TTTGACAACA TCCTGAACAG CTTTGTGACC
    TTGTTCGAGC TCACGGTTGT CAACAACTGG TACATCATCA TGGAAGGTGT CACCTCCCAG
    ACCTCGCACT GGAGCCGCCT CTACTTCATG ACCTTCTACA TCGTGACCAT GGTGGTGATG
    ACCATCATTG TGGCCTTCAT CCTCGAAGCC TTTGTCTTCC GAATGAACTA CAGCCGCAAG
    AACCAGGACT CGGAAGTGGA CGACGGCATC ACCCTGGAGA AGGAGCTCTC CAAAGACGAG
    CTGGTCGCAG TTCTGAAGCT CTACCGGGAG GCAGGGGGAG CCAACTCAGA CATTGCCCAG
    CTGCTCAAGA TCCTCTCCCA GCTGGAGAGA TACGAGCAAA ATACCTTGGT GTTTCTGGGA
    CGGAGATCAA GGACCAAAAG CGACCTGAGC CTAAAGATGT ACCAAGAGGA GATTCAGGAG
    TGGTATGAGG AACATGCCCG CAAGCAGGAG GAACGACAGC TCAGCAGCTG CGTGGTCCCT
    GCCACCCAGC AGCCTCCAGG CAGCCGCCAG CGCTCCCAGA CCATCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833525.1
    CDS352..3102
    Translation

    Target ORF information:

    RefSeq Version XM_024977757.1
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977757.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACGGAAGGT
    GAATCCTCAA CCGCTGGACC GCCAGGGACG TCCCCATCTG GGCCTTTCTT ATCTTGTGCT
    TGGATCTTGC ACGGACTTTC TTCTCAAGGC CCGCCTCCCA CCTGTCTGAA TGGTCCATCT
    AAAACACAAA TCCGAGCAGC TGCCTCCCAG GAAAGGCGGA AATGCAGAGG CGAGGGCCAG
    CCATCATCTT CCTGGAGGAG GCAGCAGAGG GCCAGCTCCC TGACAGCCCC ATGGAGAGGT
    CATCCCGTCT CCTCCAGCTG GGCCCTTGTG GTATGCCTTG ACCTGGCCGT GGATGGAGGC
    AAATATACCG TCCACGACTC CCAGGCCCCC AGTCTGAGCT TAGAGGGTGA GAACTCCCCC
    TCCAGCCCCA CTGAACATGA CTGGGAGATG AATTATCAAG AGGCAGCAAT CTACCTCCAG
    GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC TCTGGCGGCC
    TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCGCGGC CTTGCTCCTG
    CTGCTGCTCT CCCTGTGCGA GGCTCCCGCC GTCCCCGCGC TCCGGCTGGG CATTTACGTC
    CACGCAACCC TGGAGCTGTT CGCCCTGGTG GTGGTCGTGT TTGAACTCTG CATGAAGTTG
    CGGTGGCTGG GCCTTCACAC CTTCATCCGG CACAGAAGGA CCATGGTCAA GACCTCCGTG
    CTGGTGGTGC AGTTCGTGGA GGCCATCGTG GTGTTGGTAC GGCAGACGTC ACACATGCGG
    GTGACCCGGG CGCTGCGCTG CATCTTCCTG GTGGACTGTC GGTACTGCGG CGGCGTCCGG
    CGCAACCTGC GTCAGATGTT TCAGTCCCTG CCGCCCTTCA TGGACATCCT CCTGCTTCTC
    CTCTTCTTCA TGATCATTTT CGCCATCCTC GGTTTCTACT TATTCTCCCC TAATCCTTCA
    GACCCCTACT TCAGCACCCT GGAGAACAGC ATCATCAGTC TGTTTGTCCT TCTGACCACC
    GCCAACTTCC CAGACGTGAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC
    TTCATCGTGT ACCTCTCCAT TGAGCTGTAC TTTATCATGA ACCTGCTCCT GGCCGTGGTA
    TTTGACACCT TCAATGACAT CGAGAAACGC AAGTTCAAGT CTTTGCTGCT ACACAAGCGA
    ACTGCCATCC AGCACGCCTA CCGCCTGCTT GTCAGCCAGC GGAGGCCTGC CGGCATCTCC
    TACAGGCAGT TCGAAGGCCT GATGCGTTTC TACAAGCCCC GGATGAGTGC GGGGGAGCGC
    TATCTCACCT TCAAGGCCCT GAATCAGAGC AACACGCCAC TGCTCAGCCT GAAGGACTTT
    CATGATATTT ATGAAGTTGC CGCCTTAAAG TGGAAGGCAA AGAGAAATAG AGAACACTGG
    TTTGATGAGC TTCCCAGGAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAG
    TCCAAGGCCT TCCAGTATTT CATGTATTTG GTGGTAGCGG TCAACGGGGT CTGGATCCTC
    GTGGAGACCT TCATGCTGAA AGGTGGGAAC TTCGTCTCCA GGCACGTGCC GTGGAGTCAC
    CTCGTCTTTC TGACTATCTA TGCGGTAGAG CTGTTCCTGA AAGTTGCCGG CCTGGGCCCC
    ATTGAGTACC TGTCCTCAGG ATGGAACCTG TTCGACTTCT CCGTCACAGC CTTTGCCTTC
    CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTCT ACTTCATAGT GGTCCTGCGT
    CCCCTCCAGC TGCTGAGGTT GTTTAAGCTG AAGAAGCGTT ACCGCAACGT GCTGGACACC
    ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTGA CTCTGCTCAT CTTCTACTAC
    TCCTTCGCCA TCGTGGGCAT GGAATTCTTC TATGGAATCC TCTACCCCAA CTGCTGCAAT
    ACAAGCACAG TGGCAGACGC CTACCGCTGG CTGAACCACT CCGTGGGCAA CAGGACCGTC
    GTGGAGGAGG GCTATTACTA TCTCAATAAT TTTGACAACA TCCTGAACAG CTTTGTGACC
    TTGTTCGAGC TCACGGTTGT CAACAACTGG TACATCATCA TGGAAGGTGT CACCTCCCAG
    ACCTCGCACT GGAGCCGCCT CTACTTCATG ACCTTCTACA TCGTGACCAT GGTGGTGATG
    ACCATCATTG TGGCCTTCAT CCTCGAAGCC TTTGTCTTCC GAATGAACTA CAGCCGCAAG
    AACCAGGACT CGGAAGTGGA CGACGGCATC ACCCTGGAGA AGGAGCTCTC CAAAGACGAG
    CTGGTCGCAG TTCTGAAGCT CTACCGGGAG GCAGGGGGAG CCAACTCAGA CATTGCCCAG
    CTGCTCAAGA TCCTCTCCCA GCTGGAGAGA TACGAGCAAA ATACCTTGGT GTTTCTGGGA
    CGGAGATCAA GGACCAAAAG CGACCTGAGC CTAAAGATGT ACCAAGAGGA GATTCAGGAG
    TGGTATGAGG AACATGCCCG CAAGCAGGAG GAACGACAGC TCAGCAGCTG CGTGGTCCCT
    GCCACCCAGC AGCCTCCAGG CAGCCGCCAG CGCTCCCAGA CCATCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09066
    Clone ID Related Accession (Same CDS sequence) XM_024977759.1
    Accession Version XM_024977759.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2598bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACGGAAGGT
    GAATCCTCAA CCGCTGGACC GCCAGGGACG TCCCCATCTG GGCCTTTCTT ATCTTGTGCT
    TGGATCTTGC ACGGACTTTC TTCTCAAGGC CCGCCTCCCA CCTGTCTGAA TGGTCCATCT
    AAAACACAAA TCCGAGCAGA TGGAGGCAAA TATACCGTCC ACGACTCCCA GGCCCCCAGT
    CTGAGCTTAG AGGGTGAGAA CTCCCCCTCC AGCCCCACTG AACATGACTG GGAGATGAAT
    TATCAAGAGG CAGCAATCTA CCTCCAGGAA GGCGAGAATA ACGACAAGTT CTTCACCCAC
    CCCAAGAACG CCAAAGCTCT GGCGGCCTAC CTCTTCGCAC ACAACCACCT CTTCTACCTG
    ATGGAGCTGT CCGCGGCCTT GCTCCTGCTG CTGCTCTCCC TGTGCGAGGC TCCCGCCGTC
    CCCGCGCTCC GGCTGGGCAT TTACGTCCAC GCAACCCTGG AGCTGTTCGC CCTGGTGGTG
    GTCGTGTTTG AACTCTGCAT GAAGTTGCGG TGGCTGGGCC TTCACACCTT CATCCGGCAC
    AGAAGGACCA TGGTCAAGAC CTCCGTGCTG GTGGTGCAGT TCGTGGAGGC CATCGTGGTG
    TTGGTACGGC AGACGTCACA CATGCGGGTG ACCCGGGCGC TGCGCTGCAT CTTCCTGGTG
    GACTGTCGGT ACTGCGGCGG CGTCCGGCGC AACCTGCGTC AGATGTTTCA GTCCCTGCCG
    CCCTTCATGG ACATCCTCCT GCTTCTCCTC TTCTTCATGA TCATTTTCGC CATCCTCGGT
    TTCTACTTAT TCTCCCCTAA TCCTTCAGAC CCCTACTTCA GCACCCTGGA GAACAGCATC
    ATCAGTCTGT TTGTCCTTCT GACCACCGCC AACTTCCCAG ACGTGATGAT GCCCTCCTAC
    TCCCGGAACC CCTGGTCCTG CGTCTTCTTC ATCGTGTACC TCTCCATTGA GCTGTACTTT
    ATCATGAACC TGCTCCTGGC CGTGGTATTT GACACCTTCA ATGACATCGA GAAACGCAAG
    TTCAAGTCTT TGCTGCTACA CAAGCGAACT GCCATCCAGC ACGCCTACCG CCTGCTTGTC
    AGCCAGCGGA GGCCTGCCGG CATCTCCTAC AGGCAGTTCG AAGGCCTGAT GCGTTTCTAC
    AAGCCCCGGA TGAGTGCGGG GGAGCGCTAT CTCACCTTCA AGGCCCTGAA TCAGAGCAAC
    ACGCCACTGC TCAGCCTGAA GGACTTTCAT GATATTTATG AAGTTGCCGC CTTAAAGTGG
    AAGGCAAAGA GAAATAGAGA ACACTGGTTT GATGAGCTTC CCAGGACAGC ATTCCTCATC
    TTCAAAGGAA TTAATATCCT TGTGAAGTCC AAGGCCTTCC AGTATTTCAT GTATTTGGTG
    GTAGCGGTCA ACGGGGTCTG GATCCTCGTG GAGACCTTCA TGCTGAAAGG TGGGAACTTC
    GTCTCCAGGC ACGTGCCGTG GAGTCACCTC GTCTTTCTGA CTATCTATGC GGTAGAGCTG
    TTCCTGAAAG TTGCCGGCCT GGGCCCCATT GAGTACCTGT CCTCAGGATG GAACCTGTTC
    GACTTCTCCG TCACAGCCTT TGCCTTCCTG GGACTGCTGG CTCTGGCCTT CAACATGGAG
    CCCTTCTACT TCATAGTGGT CCTGCGTCCC CTCCAGCTGC TGAGGTTGTT TAAGCTGAAG
    AAGCGTTACC GCAACGTGCT GGACACCATG TTTGAGCTGC TGCCCCGGAT GGCCAGCCTG
    GGCCTGACTC TGCTCATCTT CTACTACTCC TTCGCCATCG TGGGCATGGA ATTCTTCTAT
    GGAATCCTCT ACCCCAACTG CTGCAATACA AGCACAGTGG CAGACGCCTA CCGCTGGCTG
    AACCACTCCG TGGGCAACAG GACCGTCGTG GAGGAGGGCT ATTACTATCT CAATAATTTT
    GACAACATCC TGAACAGCTT TGTGACCTTG TTCGAGCTCA CGGTTGTCAA CAACTGGTAC
    ATCATCATGG AAGGTGTCAC CTCCCAGACC TCGCACTGGA GCCGCCTCTA CTTCATGACC
    TTCTACATCG TGACCATGGT GGTGATGACC ATCATTGTGG CCTTCATCCT CGAAGCCTTT
    GTCTTCCGAA TGAACTACAG CCGCAAGAAC CAGGACTCGG AAGTGGACGA CGGCATCACC
    CTGGAGAAGG AGCTCTCCAA AGACGAGCTG GTCGCAGTTC TGAAGCTCTA CCGGGAGGCA
    GGGGGAGCCA ACTCAGACAT TGCCCAGCTG CTCAAGATCC TCTCCCAGCT GGAGAGATAC
    GAGCAAAATA CCTTGGTGTT TCTGGGACGG AGATCAAGGA CCAAAAGCGA CCTGAGCCTA
    AAGATGTACC AAGAGGAGAT TCAGGAGTGG TATGAGGAAC ATGCCCGCAA GCAGGAGGAA
    CGACAGCTCA GCAGCTGCGT GGTCCCTGCC ACCCAGCAGC CTCCAGGCAG CCGCCAGCGC
    TCCCAGACCA TCACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833527.1
    CDS351..2948
    Translation

    Target ORF information:

    RefSeq Version XM_024977759.1
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977759.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGATGA GGGTGGCAGC 
    GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CCAGATGATC TGCCCAGCAG AACGGAAGGT
    GAATCCTCAA CCGCTGGACC GCCAGGGACG TCCCCATCTG GGCCTTTCTT ATCTTGTGCT
    TGGATCTTGC ACGGACTTTC TTCTCAAGGC CCGCCTCCCA CCTGTCTGAA TGGTCCATCT
    AAAACACAAA TCCGAGCAGA TGGAGGCAAA TATACCGTCC ACGACTCCCA GGCCCCCAGT
    CTGAGCTTAG AGGGTGAGAA CTCCCCCTCC AGCCCCACTG AACATGACTG GGAGATGAAT
    TATCAAGAGG CAGCAATCTA CCTCCAGGAA GGCGAGAATA ACGACAAGTT CTTCACCCAC
    CCCAAGAACG CCAAAGCTCT GGCGGCCTAC CTCTTCGCAC ACAACCACCT CTTCTACCTG
    ATGGAGCTGT CCGCGGCCTT GCTCCTGCTG CTGCTCTCCC TGTGCGAGGC TCCCGCCGTC
    CCCGCGCTCC GGCTGGGCAT TTACGTCCAC GCAACCCTGG AGCTGTTCGC CCTGGTGGTG
    GTCGTGTTTG AACTCTGCAT GAAGTTGCGG TGGCTGGGCC TTCACACCTT CATCCGGCAC
    AGAAGGACCA TGGTCAAGAC CTCCGTGCTG GTGGTGCAGT TCGTGGAGGC CATCGTGGTG
    TTGGTACGGC AGACGTCACA CATGCGGGTG ACCCGGGCGC TGCGCTGCAT CTTCCTGGTG
    GACTGTCGGT ACTGCGGCGG CGTCCGGCGC AACCTGCGTC AGATGTTTCA GTCCCTGCCG
    CCCTTCATGG ACATCCTCCT GCTTCTCCTC TTCTTCATGA TCATTTTCGC CATCCTCGGT
    TTCTACTTAT TCTCCCCTAA TCCTTCAGAC CCCTACTTCA GCACCCTGGA GAACAGCATC
    ATCAGTCTGT TTGTCCTTCT GACCACCGCC AACTTCCCAG ACGTGATGAT GCCCTCCTAC
    TCCCGGAACC CCTGGTCCTG CGTCTTCTTC ATCGTGTACC TCTCCATTGA GCTGTACTTT
    ATCATGAACC TGCTCCTGGC CGTGGTATTT GACACCTTCA ATGACATCGA GAAACGCAAG
    TTCAAGTCTT TGCTGCTACA CAAGCGAACT GCCATCCAGC ACGCCTACCG CCTGCTTGTC
    AGCCAGCGGA GGCCTGCCGG CATCTCCTAC AGGCAGTTCG AAGGCCTGAT GCGTTTCTAC
    AAGCCCCGGA TGAGTGCGGG GGAGCGCTAT CTCACCTTCA AGGCCCTGAA TCAGAGCAAC
    ACGCCACTGC TCAGCCTGAA GGACTTTCAT GATATTTATG AAGTTGCCGC CTTAAAGTGG
    AAGGCAAAGA GAAATAGAGA ACACTGGTTT GATGAGCTTC CCAGGACAGC ATTCCTCATC
    TTCAAAGGAA TTAATATCCT TGTGAAGTCC AAGGCCTTCC AGTATTTCAT GTATTTGGTG
    GTAGCGGTCA ACGGGGTCTG GATCCTCGTG GAGACCTTCA TGCTGAAAGG TGGGAACTTC
    GTCTCCAGGC ACGTGCCGTG GAGTCACCTC GTCTTTCTGA CTATCTATGC GGTAGAGCTG
    TTCCTGAAAG TTGCCGGCCT GGGCCCCATT GAGTACCTGT CCTCAGGATG GAACCTGTTC
    GACTTCTCCG TCACAGCCTT TGCCTTCCTG GGACTGCTGG CTCTGGCCTT CAACATGGAG
    CCCTTCTACT TCATAGTGGT CCTGCGTCCC CTCCAGCTGC TGAGGTTGTT TAAGCTGAAG
    AAGCGTTACC GCAACGTGCT GGACACCATG TTTGAGCTGC TGCCCCGGAT GGCCAGCCTG
    GGCCTGACTC TGCTCATCTT CTACTACTCC TTCGCCATCG TGGGCATGGA ATTCTTCTAT
    GGAATCCTCT ACCCCAACTG CTGCAATACA AGCACAGTGG CAGACGCCTA CCGCTGGCTG
    AACCACTCCG TGGGCAACAG GACCGTCGTG GAGGAGGGCT ATTACTATCT CAATAATTTT
    GACAACATCC TGAACAGCTT TGTGACCTTG TTCGAGCTCA CGGTTGTCAA CAACTGGTAC
    ATCATCATGG AAGGTGTCAC CTCCCAGACC TCGCACTGGA GCCGCCTCTA CTTCATGACC
    TTCTACATCG TGACCATGGT GGTGATGACC ATCATTGTGG CCTTCATCCT CGAAGCCTTT
    GTCTTCCGAA TGAACTACAG CCGCAAGAAC CAGGACTCGG AAGTGGACGA CGGCATCACC
    CTGGAGAAGG AGCTCTCCAA AGACGAGCTG GTCGCAGTTC TGAAGCTCTA CCGGGAGGCA
    GGGGGAGCCA ACTCAGACAT TGCCCAGCTG CTCAAGATCC TCTCCCAGCT GGAGAGATAC
    GAGCAAAATA CCTTGGTGTT TCTGGGACGG AGATCAAGGA CCAAAAGCGA CCTGAGCCTA
    AAGATGTACC AAGAGGAGAT TCAGGAGTGG TATGAGGAAC ATGCCCGCAA GCAGGAGGAA
    CGACAGCTCA GCAGCTGCGT GGTCCCTGCC ACCCAGCAGC CTCCAGGCAG CCGCCAGCGC
    TCCCAGACCA TCACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93226
    Clone ID Related Accession (Same CDS sequence) XM_015467156.1 , XM_015475525.1
    Accession Version XM_015467156.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2448bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCAGAGGC GAGGGCCAGC CATCATCTTC CTGGAGGAGG CAGCAGAGGG CCAGCTCCCT 
    GACAGCCCCA TGGAGAGGTC ATCCCGTCTC CTCCAGCTGG GCCCTTGTGA TGGAGGCAAA
    TATACCGTCC ACGACTCCCA GGCCCCCAGT CTGAGCTTAG AGGGTGAGAA CTCCCCCTCC
    AGCCCCACTG AACATGACTG GGAGATGAAT TATCAAGAGG CAGCAATCTA CCTCCAGGAA
    GGCGAGAATA ACGACAAGTT CTTCACCCAC CCCAAGAACG CCAAAGCTCT GGCGGCCTAC
    CTCTTCGCAC ACAACCACCT CTTCTACCTG ATGGAGCTGT CCGCGGCCTT GCTCCTGCTG
    CTGCTCTCCC TGTGCGAGGC TCCCGCCGTC CCCGCGCTCC GGCTGGGCAT TTACGTCCAC
    GCAACCCTGG AGCTGTTCGC CCTGGTGGTG GTCGTGTTTG AACTCTGCAT GAAGTTGCGG
    TGGCTGGGCC TTCACACCTT CATCCGGCAC AGAAGGACCA TGGTCAAGAC CTCCGTGCTG
    GTGGTGCAGT TCGTGGAGGC CATCGTGGTG TTGGTACGGC AGACGTCACA CATGCGGGTG
    ACCCGGGCGC TGCGCTGCAT CTTCCTGGTG GACTGTCGGT ACTGCGGCGG CGTCCGGCGC
    AACCTGCGTC AGATGTTTCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTTCTCCTC
    TTCTTCATGA TCATTTTCGC CATCCTCGGT TTCTACTTAT TCTCCCCTAA TCCTTCAGAC
    CCCTACTTCA GCACCCTGGA GAACAGCATC ATCAGTCTGT TTGTCCTTCT GACCACCGCC
    AACTTCCCAG ACGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC
    ATCGTGTACC TCTCCATTGA GCTGTACTTT ATCATGAACC TGCTCCTGGC CGTGGTATTT
    GACACCTTCA ATGACATCGA GAAACGCAAG TTCAAGTCTT TGCTGCTACA CAAGCGAACT
    GCCATCCAGC ACGCCTACCG CCTGCTTGTC AGCCAGCGGA GGCCTGCCGG CATCTCCTAC
    AGGCAGTTCG AAGGCCTGAT GCGTTTCTAC AAGCCCCGGA TGAGTGCGGG GGAGCGCTAT
    CTCACCTTCA AGGCCCTGAA TCAGAGCAAC ACGCCACTGC TCAGCCTGAA GGACTTTCAT
    GATATTTATG AAGTTGCCGC CTTAAAGTGG AAGGCAAAGA GAAATAGAGA ACACTGGTTT
    GATGAGCTTC CCAGGACAGC ATTCCTCATC TTCAAAGGAA TTAATATCCT TGTGAAGTCC
    AAGGCCTTCC AGTATTTCAT GTATTTGGTG GTAGCGGTCA ACGGGGTCTG GATCCTCGTG
    GAGACCTTCA TGCTGAAAGG TGGGAACTTC GTCTCCAGGC ACGTGCCGTG GAGTCACCTC
    GTCTTTCTGA CTATCTATGC GGTAGAGCTG TTCCTGAAAG TTGCCGGCCT GGGCCCCATT
    GAGTACCTGT CCTCAGGATG GAACCTGTTC GACTTCTCCG TCACAGCCTT TGCCTTCCTG
    GGACTGCTGG CTCTGGCCTT CAACATGGAG CCCTTCTACT TCATAGTGGT CCTGCGTCCC
    CTCCAGCTGC TGAGGTTGTT TAAGCTGAAG AAGCGTTACC GCAACGTGCT GGACACCATG
    TTTGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTGACTC TGCTCATCTT CTACTACTCC
    TTCGCCATCG TGGGCATGGA ATTCTTCTAT GGAATCCTCT ACCCCAACTG CTGCAATACA
    AGCACAGTGG CAGACGCCTA CCGCTGGCTG AACCACTCCG TGGGCAACAG GACCGTCGTG
    GAGGAGGGCT ATTACTATCT CAATAATTTT GACAACATCC TGAACAGCTT TGTGACCTTG
    TTCGAGCTCA CGGTTGTCAA CAACTGGTAC ATCATCATGG AAGGTGTCAC CTCCCAGACC
    TCGCACTGGA GCCGCCTCTA CTTCATGACC TTCTACATCG TGACCATGGT GGTGATGACC
    ATCATTGTGG CCTTCATCCT CGAAGCCTTT GTCTTCCGAA TGAACTACAG CCGCAAGAAC
    CAGGACTCGG AAGTGGACGA CGGCATCACC CTGGAGAAGG AGCTCTCCAA AGACGAGCTG
    GTCGCAGTTC TGAAGCTCTA CCGGGAGGCA GGGGGAGCCA ACTCAGACAT TGCCCAGCTG
    CTCAAGATCC TCTCCCAGCT GGAGAGATAC GAGCAAAATA CCTTGGTGTT TCTGGGACGG
    AGATCAAGGA CCAAAAGCGA CCTGAGCCTA AAGATGTACC AAGAGGAGAT TCAGGAGTGG
    TATGAGGAAC ATGCCCGCAA GCAGGAGGAA CGACAGCTCA GCAGCTGCGT GGTCCCTGCC
    ACCCAGCAGC CTCCAGGCAG CCGCCAGCGC TCCCAGACCA TCACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322642.1
    CDS125..2572
    Translation

    Target ORF information:

    RefSeq Version XM_015467156.1
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467156.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCAGAGGC GAGGGCCAGC CATCATCTTC CTGGAGGAGG CAGCAGAGGG CCAGCTCCCT 
    GACAGCCCCA TGGAGAGGTC ATCCCGTCTC CTCCAGCTGG GCCCTTGTGA TGGAGGCAAA
    TATACCGTCC ACGACTCCCA GGCCCCCAGT CTGAGCTTAG AGGGTGAGAA CTCCCCCTCC
    AGCCCCACTG AACATGACTG GGAGATGAAT TATCAAGAGG CAGCAATCTA CCTCCAGGAA
    GGCGAGAATA ACGACAAGTT CTTCACCCAC CCCAAGAACG CCAAAGCTCT GGCGGCCTAC
    CTCTTCGCAC ACAACCACCT CTTCTACCTG ATGGAGCTGT CCGCGGCCTT GCTCCTGCTG
    CTGCTCTCCC TGTGCGAGGC TCCCGCCGTC CCCGCGCTCC GGCTGGGCAT TTACGTCCAC
    GCAACCCTGG AGCTGTTCGC CCTGGTGGTG GTCGTGTTTG AACTCTGCAT GAAGTTGCGG
    TGGCTGGGCC TTCACACCTT CATCCGGCAC AGAAGGACCA TGGTCAAGAC CTCCGTGCTG
    GTGGTGCAGT TCGTGGAGGC CATCGTGGTG TTGGTACGGC AGACGTCACA CATGCGGGTG
    ACCCGGGCGC TGCGCTGCAT CTTCCTGGTG GACTGTCGGT ACTGCGGCGG CGTCCGGCGC
    AACCTGCGTC AGATGTTTCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTTCTCCTC
    TTCTTCATGA TCATTTTCGC CATCCTCGGT TTCTACTTAT TCTCCCCTAA TCCTTCAGAC
    CCCTACTTCA GCACCCTGGA GAACAGCATC ATCAGTCTGT TTGTCCTTCT GACCACCGCC
    AACTTCCCAG ACGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC
    ATCGTGTACC TCTCCATTGA GCTGTACTTT ATCATGAACC TGCTCCTGGC CGTGGTATTT
    GACACCTTCA ATGACATCGA GAAACGCAAG TTCAAGTCTT TGCTGCTACA CAAGCGAACT
    GCCATCCAGC ACGCCTACCG CCTGCTTGTC AGCCAGCGGA GGCCTGCCGG CATCTCCTAC
    AGGCAGTTCG AAGGCCTGAT GCGTTTCTAC AAGCCCCGGA TGAGTGCGGG GGAGCGCTAT
    CTCACCTTCA AGGCCCTGAA TCAGAGCAAC ACGCCACTGC TCAGCCTGAA GGACTTTCAT
    GATATTTATG AAGTTGCCGC CTTAAAGTGG AAGGCAAAGA GAAATAGAGA ACACTGGTTT
    GATGAGCTTC CCAGGACAGC ATTCCTCATC TTCAAAGGAA TTAATATCCT TGTGAAGTCC
    AAGGCCTTCC AGTATTTCAT GTATTTGGTG GTAGCGGTCA ACGGGGTCTG GATCCTCGTG
    GAGACCTTCA TGCTGAAAGG TGGGAACTTC GTCTCCAGGC ACGTGCCGTG GAGTCACCTC
    GTCTTTCTGA CTATCTATGC GGTAGAGCTG TTCCTGAAAG TTGCCGGCCT GGGCCCCATT
    GAGTACCTGT CCTCAGGATG GAACCTGTTC GACTTCTCCG TCACAGCCTT TGCCTTCCTG
    GGACTGCTGG CTCTGGCCTT CAACATGGAG CCCTTCTACT TCATAGTGGT CCTGCGTCCC
    CTCCAGCTGC TGAGGTTGTT TAAGCTGAAG AAGCGTTACC GCAACGTGCT GGACACCATG
    TTTGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTGACTC TGCTCATCTT CTACTACTCC
    TTCGCCATCG TGGGCATGGA ATTCTTCTAT GGAATCCTCT ACCCCAACTG CTGCAATACA
    AGCACAGTGG CAGACGCCTA CCGCTGGCTG AACCACTCCG TGGGCAACAG GACCGTCGTG
    GAGGAGGGCT ATTACTATCT CAATAATTTT GACAACATCC TGAACAGCTT TGTGACCTTG
    TTCGAGCTCA CGGTTGTCAA CAACTGGTAC ATCATCATGG AAGGTGTCAC CTCCCAGACC
    TCGCACTGGA GCCGCCTCTA CTTCATGACC TTCTACATCG TGACCATGGT GGTGATGACC
    ATCATTGTGG CCTTCATCCT CGAAGCCTTT GTCTTCCGAA TGAACTACAG CCGCAAGAAC
    CAGGACTCGG AAGTGGACGA CGGCATCACC CTGGAGAAGG AGCTCTCCAA AGACGAGCTG
    GTCGCAGTTC TGAAGCTCTA CCGGGAGGCA GGGGGAGCCA ACTCAGACAT TGCCCAGCTG
    CTCAAGATCC TCTCCCAGCT GGAGAGATAC GAGCAAAATA CCTTGGTGTT TCTGGGACGG
    AGATCAAGGA CCAAAAGCGA CCTGAGCCTA AAGATGTACC AAGAGGAGAT TCAGGAGTGG
    TATGAGGAAC ATGCCCGCAA GCAGGAGGAA CGACAGCTCA GCAGCTGCGT GGTCCCTGCC
    ACCCAGCAGC CTCCAGGCAG CCGCCAGCGC TCCCAGACCA TCACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93226
    Clone ID Related Accession (Same CDS sequence) XM_015467156.1 , XM_015475525.1
    Accession Version XM_015475525.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2448bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCAGAGGC GAGGGCCAGC CATCATCTTC CTGGAGGAGG CAGCAGAGGG CCAGCTCCCT 
    GACAGCCCCA TGGAGAGGTC ATCCCGTCTC CTCCAGCTGG GCCCTTGTGA TGGAGGCAAA
    TATACCGTCC ACGACTCCCA GGCCCCCAGT CTGAGCTTAG AGGGTGAGAA CTCCCCCTCC
    AGCCCCACTG AACATGACTG GGAGATGAAT TATCAAGAGG CAGCAATCTA CCTCCAGGAA
    GGCGAGAATA ACGACAAGTT CTTCACCCAC CCCAAGAACG CCAAAGCTCT GGCGGCCTAC
    CTCTTCGCAC ACAACCACCT CTTCTACCTG ATGGAGCTGT CCGCGGCCTT GCTCCTGCTG
    CTGCTCTCCC TGTGCGAGGC TCCCGCCGTC CCCGCGCTCC GGCTGGGCAT TTACGTCCAC
    GCAACCCTGG AGCTGTTCGC CCTGGTGGTG GTCGTGTTTG AACTCTGCAT GAAGTTGCGG
    TGGCTGGGCC TTCACACCTT CATCCGGCAC AGAAGGACCA TGGTCAAGAC CTCCGTGCTG
    GTGGTGCAGT TCGTGGAGGC CATCGTGGTG TTGGTACGGC AGACGTCACA CATGCGGGTG
    ACCCGGGCGC TGCGCTGCAT CTTCCTGGTG GACTGTCGGT ACTGCGGCGG CGTCCGGCGC
    AACCTGCGTC AGATGTTTCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTTCTCCTC
    TTCTTCATGA TCATTTTCGC CATCCTCGGT TTCTACTTAT TCTCCCCTAA TCCTTCAGAC
    CCCTACTTCA GCACCCTGGA GAACAGCATC ATCAGTCTGT TTGTCCTTCT GACCACCGCC
    AACTTCCCAG ACGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC
    ATCGTGTACC TCTCCATTGA GCTGTACTTT ATCATGAACC TGCTCCTGGC CGTGGTATTT
    GACACCTTCA ATGACATCGA GAAACGCAAG TTCAAGTCTT TGCTGCTACA CAAGCGAACT
    GCCATCCAGC ACGCCTACCG CCTGCTTGTC AGCCAGCGGA GGCCTGCCGG CATCTCCTAC
    AGGCAGTTCG AAGGCCTGAT GCGTTTCTAC AAGCCCCGGA TGAGTGCGGG GGAGCGCTAT
    CTCACCTTCA AGGCCCTGAA TCAGAGCAAC ACGCCACTGC TCAGCCTGAA GGACTTTCAT
    GATATTTATG AAGTTGCCGC CTTAAAGTGG AAGGCAAAGA GAAATAGAGA ACACTGGTTT
    GATGAGCTTC CCAGGACAGC ATTCCTCATC TTCAAAGGAA TTAATATCCT TGTGAAGTCC
    AAGGCCTTCC AGTATTTCAT GTATTTGGTG GTAGCGGTCA ACGGGGTCTG GATCCTCGTG
    GAGACCTTCA TGCTGAAAGG TGGGAACTTC GTCTCCAGGC ACGTGCCGTG GAGTCACCTC
    GTCTTTCTGA CTATCTATGC GGTAGAGCTG TTCCTGAAAG TTGCCGGCCT GGGCCCCATT
    GAGTACCTGT CCTCAGGATG GAACCTGTTC GACTTCTCCG TCACAGCCTT TGCCTTCCTG
    GGACTGCTGG CTCTGGCCTT CAACATGGAG CCCTTCTACT TCATAGTGGT CCTGCGTCCC
    CTCCAGCTGC TGAGGTTGTT TAAGCTGAAG AAGCGTTACC GCAACGTGCT GGACACCATG
    TTTGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTGACTC TGCTCATCTT CTACTACTCC
    TTCGCCATCG TGGGCATGGA ATTCTTCTAT GGAATCCTCT ACCCCAACTG CTGCAATACA
    AGCACAGTGG CAGACGCCTA CCGCTGGCTG AACCACTCCG TGGGCAACAG GACCGTCGTG
    GAGGAGGGCT ATTACTATCT CAATAATTTT GACAACATCC TGAACAGCTT TGTGACCTTG
    TTCGAGCTCA CGGTTGTCAA CAACTGGTAC ATCATCATGG AAGGTGTCAC CTCCCAGACC
    TCGCACTGGA GCCGCCTCTA CTTCATGACC TTCTACATCG TGACCATGGT GGTGATGACC
    ATCATTGTGG CCTTCATCCT CGAAGCCTTT GTCTTCCGAA TGAACTACAG CCGCAAGAAC
    CAGGACTCGG AAGTGGACGA CGGCATCACC CTGGAGAAGG AGCTCTCCAA AGACGAGCTG
    GTCGCAGTTC TGAAGCTCTA CCGGGAGGCA GGGGGAGCCA ACTCAGACAT TGCCCAGCTG
    CTCAAGATCC TCTCCCAGCT GGAGAGATAC GAGCAAAATA CCTTGGTGTT TCTGGGACGG
    AGATCAAGGA CCAAAAGCGA CCTGAGCCTA AAGATGTACC AAGAGGAGAT TCAGGAGTGG
    TATGAGGAAC ATGCCCGCAA GCAGGAGGAA CGACAGCTCA GCAGCTGCGT GGTCCCTGCC
    ACCCAGCAGC CTCCAGGCAG CCGCCAGCGC TCCCAGACCA TCACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331011.1
    CDS125..2572
    Translation

    Target ORF information:

    RefSeq Version XM_015475525.1
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475525.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCAGAGGC GAGGGCCAGC CATCATCTTC CTGGAGGAGG CAGCAGAGGG CCAGCTCCCT 
    GACAGCCCCA TGGAGAGGTC ATCCCGTCTC CTCCAGCTGG GCCCTTGTGA TGGAGGCAAA
    TATACCGTCC ACGACTCCCA GGCCCCCAGT CTGAGCTTAG AGGGTGAGAA CTCCCCCTCC
    AGCCCCACTG AACATGACTG GGAGATGAAT TATCAAGAGG CAGCAATCTA CCTCCAGGAA
    GGCGAGAATA ACGACAAGTT CTTCACCCAC CCCAAGAACG CCAAAGCTCT GGCGGCCTAC
    CTCTTCGCAC ACAACCACCT CTTCTACCTG ATGGAGCTGT CCGCGGCCTT GCTCCTGCTG
    CTGCTCTCCC TGTGCGAGGC TCCCGCCGTC CCCGCGCTCC GGCTGGGCAT TTACGTCCAC
    GCAACCCTGG AGCTGTTCGC CCTGGTGGTG GTCGTGTTTG AACTCTGCAT GAAGTTGCGG
    TGGCTGGGCC TTCACACCTT CATCCGGCAC AGAAGGACCA TGGTCAAGAC CTCCGTGCTG
    GTGGTGCAGT TCGTGGAGGC CATCGTGGTG TTGGTACGGC AGACGTCACA CATGCGGGTG
    ACCCGGGCGC TGCGCTGCAT CTTCCTGGTG GACTGTCGGT ACTGCGGCGG CGTCCGGCGC
    AACCTGCGTC AGATGTTTCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTTCTCCTC
    TTCTTCATGA TCATTTTCGC CATCCTCGGT TTCTACTTAT TCTCCCCTAA TCCTTCAGAC
    CCCTACTTCA GCACCCTGGA GAACAGCATC ATCAGTCTGT TTGTCCTTCT GACCACCGCC
    AACTTCCCAG ACGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC
    ATCGTGTACC TCTCCATTGA GCTGTACTTT ATCATGAACC TGCTCCTGGC CGTGGTATTT
    GACACCTTCA ATGACATCGA GAAACGCAAG TTCAAGTCTT TGCTGCTACA CAAGCGAACT
    GCCATCCAGC ACGCCTACCG CCTGCTTGTC AGCCAGCGGA GGCCTGCCGG CATCTCCTAC
    AGGCAGTTCG AAGGCCTGAT GCGTTTCTAC AAGCCCCGGA TGAGTGCGGG GGAGCGCTAT
    CTCACCTTCA AGGCCCTGAA TCAGAGCAAC ACGCCACTGC TCAGCCTGAA GGACTTTCAT
    GATATTTATG AAGTTGCCGC CTTAAAGTGG AAGGCAAAGA GAAATAGAGA ACACTGGTTT
    GATGAGCTTC CCAGGACAGC ATTCCTCATC TTCAAAGGAA TTAATATCCT TGTGAAGTCC
    AAGGCCTTCC AGTATTTCAT GTATTTGGTG GTAGCGGTCA ACGGGGTCTG GATCCTCGTG
    GAGACCTTCA TGCTGAAAGG TGGGAACTTC GTCTCCAGGC ACGTGCCGTG GAGTCACCTC
    GTCTTTCTGA CTATCTATGC GGTAGAGCTG TTCCTGAAAG TTGCCGGCCT GGGCCCCATT
    GAGTACCTGT CCTCAGGATG GAACCTGTTC GACTTCTCCG TCACAGCCTT TGCCTTCCTG
    GGACTGCTGG CTCTGGCCTT CAACATGGAG CCCTTCTACT TCATAGTGGT CCTGCGTCCC
    CTCCAGCTGC TGAGGTTGTT TAAGCTGAAG AAGCGTTACC GCAACGTGCT GGACACCATG
    TTTGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTGACTC TGCTCATCTT CTACTACTCC
    TTCGCCATCG TGGGCATGGA ATTCTTCTAT GGAATCCTCT ACCCCAACTG CTGCAATACA
    AGCACAGTGG CAGACGCCTA CCGCTGGCTG AACCACTCCG TGGGCAACAG GACCGTCGTG
    GAGGAGGGCT ATTACTATCT CAATAATTTT GACAACATCC TGAACAGCTT TGTGACCTTG
    TTCGAGCTCA CGGTTGTCAA CAACTGGTAC ATCATCATGG AAGGTGTCAC CTCCCAGACC
    TCGCACTGGA GCCGCCTCTA CTTCATGACC TTCTACATCG TGACCATGGT GGTGATGACC
    ATCATTGTGG CCTTCATCCT CGAAGCCTTT GTCTTCCGAA TGAACTACAG CCGCAAGAAC
    CAGGACTCGG AAGTGGACGA CGGCATCACC CTGGAGAAGG AGCTCTCCAA AGACGAGCTG
    GTCGCAGTTC TGAAGCTCTA CCGGGAGGCA GGGGGAGCCA ACTCAGACAT TGCCCAGCTG
    CTCAAGATCC TCTCCCAGCT GGAGAGATAC GAGCAAAATA CCTTGGTGTT TCTGGGACGG
    AGATCAAGGA CCAAAAGCGA CCTGAGCCTA AAGATGTACC AAGAGGAGAT TCAGGAGTGG
    TATGAGGAAC ATGCCCGCAA GCAGGAGGAA CGACAGCTCA GCAGCTGCGT GGTCCCTGCC
    ACCCAGCAGC CTCCAGGCAG CCGCCAGCGC TCCCAGACCA TCACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93227
    Clone ID Related Accession (Same CDS sequence) XM_015475526.1 , XM_015467157.1 , XM_015475526.2
    Accession Version XM_015475526.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGGGTGG CAGCGCCCCA CTGGCTCCCT CCAACGGCCT GGGCCCAGAT GATCTGCCCA 
    GCAGAAGAAG GCGAGAATAA CGACAAGTTC TTCACCCACC CCAAGAACGC CAAAGCTCTG
    GCGGCCTACC TCTTCGCACA CAACCACCTC TTCTACCTGA TGGAGCTGTC CGCGGCCTTG
    CTCCTGCTGC TGCTCTCCCT GTGCGAGGCT CCCGCCGTCC CCGCGCTCCG GCTGGGCATT
    TACGTCCACG CAACCCTGGA GCTGTTCGCC CTGGTGGTGG TCGTGTTTGA ACTCTGCATG
    AAGTTGCGGT GGCTGGGCCT TCACACCTTC ATCCGGCACA GAAGGACCAT GGTCAAGACC
    TCCGTGCTGG TGGTGCAGTT CGTGGAGGCC ATCGTGGTGT TGGTACGGCA GACGTCACAC
    ATGCGGGTGA CCCGGGCGCT GCGCTGCATC TTCCTGGTGG ACTGTCGGTA CTGCGGCGGC
    GTCCGGCGCA ACCTGCGTCA GATGTTTCAG TCCCTGCCGC CCTTCATGGA CATCCTCCTG
    CTTCTCCTCT TCTTCATGAT CATTTTCGCC ATCCTCGGTT TCTACTTATT CTCCCCTAAT
    CCTTCAGACC CCTACTTCAG CACCCTGGAG AACAGCATCA TCAGTCTGTT TGTCCTTCTG
    ACCACCGCCA ACTTCCCAGA CGTGATGATG CCCTCCTACT CCCGGAACCC CTGGTCCTGC
    GTCTTCTTCA TCGTGTACCT CTCCATTGAG CTGTACTTTA TCATGAACCT GCTCCTGGCC
    GTGGTATTTG ACACCTTCAA TGACATCGAG AAACGCAAGT TCAAGTCTTT GCTGCTACAC
    AAGCGAACTG CCATCCAGCA CGCCTACCGC CTGCTTGTCA GCCAGCGGAG GCCTGCCGGC
    ATCTCCTACA GGCAGTTCGA AGGCCTGATG CGTTTCTACA AGCCCCGGAT GAGTGCGGGG
    GAGCGCTATC TCACCTTCAA GGCCCTGAAT CAGAGCAACA CGCCACTGCT CAGCCTGAAG
    GACTTTCATG ATATTTATGA AGTTGCCGCC TTAAAGTGGA AGGCAAAGAG AAATAGAGAA
    CACTGGTTTG ATGAGCTTCC CAGGACAGCA TTCCTCATCT TCAAAGGAAT TAATATCCTT
    GTGAAGTCCA AGGCCTTCCA GTATTTCATG TATTTGGTGG TAGCGGTCAA CGGGGTCTGG
    ATCCTCGTGG AGACCTTCAT GCTGAAAGGT GGGAACTTCG TCTCCAGGCA CGTGCCGTGG
    AGTCACCTCG TCTTTCTGAC TATCTATGCG GTAGAGCTGT TCCTGAAAGT TGCCGGCCTG
    GGCCCCATTG AGTACCTGTC CTCAGGATGG AACCTGTTCG ACTTCTCCGT CACAGCCTTT
    GCCTTCCTGG GACTGCTGGC TCTGGCCTTC AACATGGAGC CCTTCTACTT CATAGTGGTC
    CTGCGTCCCC TCCAGCTGCT GAGGTTGTTT AAGCTGAAGA AGCGTTACCG CAACGTGCTG
    GACACCATGT TTGAGCTGCT GCCCCGGATG GCCAGCCTGG GCCTGACTCT GCTCATCTTC
    TACTACTCCT TCGCCATCGT GGGCATGGAA TTCTTCTATG GAATCCTCTA CCCCAACTGC
    TGCAATACAA GCACAGTGGC AGACGCCTAC CGCTGGCTGA ACCACTCCGT GGGCAACAGG
    ACCGTCGTGG AGGAGGGCTA TTACTATCTC AATAATTTTG ACAACATCCT GAACAGCTTT
    GTGACCTTGT TCGAGCTCAC GGTTGTCAAC AACTGGTACA TCATCATGGA AGGTGTCACC
    TCCCAGACCT CGCACTGGAG CCGCCTCTAC TTCATGACCT TCTACATCGT GACCATGGTG
    GTGATGACCA TCATTGTGGC CTTCATCCTC GAAGCCTTTG TCTTCCGAAT GAACTACAGC
    CGCAAGAACC AGGACTCGGA AGTGGACGAC GGCATCACCC TGGAGAAGGA GCTCTCCAAA
    GACGAGCTGG TCGCAGTTCT GAAGCTCTAC CGGGAGGCAG GGGGAGCCAA CTCAGACATT
    GCCCAGCTGC TCAAGATCCT CTCCCAGCTG GAGAGATACG AGCAAAATAC CTTGGTGTTT
    CTGGGACGGA GATCAAGGAC CAAAAGCGAC CTGAGCCTAA AGATGTACCA AGAGGAGATT
    CAGGAGTGGT ATGAGGAACA TGCCCGCAAG CAGGAGGAAC GACAGCTCAG CAGCTGCGTG
    GTCCCTGCCA CCCAGCAGCC TCCAGGCAGC CGCCAGCGCT CCCAGACCAT CACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331012.1
    CDS36..2312
    Translation

    Target ORF information:

    RefSeq Version XM_015475526.1
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGGGTGG CAGCGCCCCA CTGGCTCCCT CCAACGGCCT GGGCCCAGAT GATCTGCCCA 
    GCAGAAGAAG GCGAGAATAA CGACAAGTTC TTCACCCACC CCAAGAACGC CAAAGCTCTG
    GCGGCCTACC TCTTCGCACA CAACCACCTC TTCTACCTGA TGGAGCTGTC CGCGGCCTTG
    CTCCTGCTGC TGCTCTCCCT GTGCGAGGCT CCCGCCGTCC CCGCGCTCCG GCTGGGCATT
    TACGTCCACG CAACCCTGGA GCTGTTCGCC CTGGTGGTGG TCGTGTTTGA ACTCTGCATG
    AAGTTGCGGT GGCTGGGCCT TCACACCTTC ATCCGGCACA GAAGGACCAT GGTCAAGACC
    TCCGTGCTGG TGGTGCAGTT CGTGGAGGCC ATCGTGGTGT TGGTACGGCA GACGTCACAC
    ATGCGGGTGA CCCGGGCGCT GCGCTGCATC TTCCTGGTGG ACTGTCGGTA CTGCGGCGGC
    GTCCGGCGCA ACCTGCGTCA GATGTTTCAG TCCCTGCCGC CCTTCATGGA CATCCTCCTG
    CTTCTCCTCT TCTTCATGAT CATTTTCGCC ATCCTCGGTT TCTACTTATT CTCCCCTAAT
    CCTTCAGACC CCTACTTCAG CACCCTGGAG AACAGCATCA TCAGTCTGTT TGTCCTTCTG
    ACCACCGCCA ACTTCCCAGA CGTGATGATG CCCTCCTACT CCCGGAACCC CTGGTCCTGC
    GTCTTCTTCA TCGTGTACCT CTCCATTGAG CTGTACTTTA TCATGAACCT GCTCCTGGCC
    GTGGTATTTG ACACCTTCAA TGACATCGAG AAACGCAAGT TCAAGTCTTT GCTGCTACAC
    AAGCGAACTG CCATCCAGCA CGCCTACCGC CTGCTTGTCA GCCAGCGGAG GCCTGCCGGC
    ATCTCCTACA GGCAGTTCGA AGGCCTGATG CGTTTCTACA AGCCCCGGAT GAGTGCGGGG
    GAGCGCTATC TCACCTTCAA GGCCCTGAAT CAGAGCAACA CGCCACTGCT CAGCCTGAAG
    GACTTTCATG ATATTTATGA AGTTGCCGCC TTAAAGTGGA AGGCAAAGAG AAATAGAGAA
    CACTGGTTTG ATGAGCTTCC CAGGACAGCA TTCCTCATCT TCAAAGGAAT TAATATCCTT
    GTGAAGTCCA AGGCCTTCCA GTATTTCATG TATTTGGTGG TAGCGGTCAA CGGGGTCTGG
    ATCCTCGTGG AGACCTTCAT GCTGAAAGGT GGGAACTTCG TCTCCAGGCA CGTGCCGTGG
    AGTCACCTCG TCTTTCTGAC TATCTATGCG GTAGAGCTGT TCCTGAAAGT TGCCGGCCTG
    GGCCCCATTG AGTACCTGTC CTCAGGATGG AACCTGTTCG ACTTCTCCGT CACAGCCTTT
    GCCTTCCTGG GACTGCTGGC TCTGGCCTTC AACATGGAGC CCTTCTACTT CATAGTGGTC
    CTGCGTCCCC TCCAGCTGCT GAGGTTGTTT AAGCTGAAGA AGCGTTACCG CAACGTGCTG
    GACACCATGT TTGAGCTGCT GCCCCGGATG GCCAGCCTGG GCCTGACTCT GCTCATCTTC
    TACTACTCCT TCGCCATCGT GGGCATGGAA TTCTTCTATG GAATCCTCTA CCCCAACTGC
    TGCAATACAA GCACAGTGGC AGACGCCTAC CGCTGGCTGA ACCACTCCGT GGGCAACAGG
    ACCGTCGTGG AGGAGGGCTA TTACTATCTC AATAATTTTG ACAACATCCT GAACAGCTTT
    GTGACCTTGT TCGAGCTCAC GGTTGTCAAC AACTGGTACA TCATCATGGA AGGTGTCACC
    TCCCAGACCT CGCACTGGAG CCGCCTCTAC TTCATGACCT TCTACATCGT GACCATGGTG
    GTGATGACCA TCATTGTGGC CTTCATCCTC GAAGCCTTTG TCTTCCGAAT GAACTACAGC
    CGCAAGAACC AGGACTCGGA AGTGGACGAC GGCATCACCC TGGAGAAGGA GCTCTCCAAA
    GACGAGCTGG TCGCAGTTCT GAAGCTCTAC CGGGAGGCAG GGGGAGCCAA CTCAGACATT
    GCCCAGCTGC TCAAGATCCT CTCCCAGCTG GAGAGATACG AGCAAAATAC CTTGGTGTTT
    CTGGGACGGA GATCAAGGAC CAAAAGCGAC CTGAGCCTAA AGATGTACCA AGAGGAGATT
    CAGGAGTGGT ATGAGGAACA TGCCCGCAAG CAGGAGGAAC GACAGCTCAG CAGCTGCGTG
    GTCCCTGCCA CCCAGCAGCC TCCAGGCAGC CGCCAGCGCT CCCAGACCAT CACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93227
    Clone ID Related Accession (Same CDS sequence) XM_015475526.1 , XM_015467157.1 , XM_015475526.2
    Accession Version XM_015467157.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGGGTGG CAGCGCCCCA CTGGCTCCCT CCAACGGCCT GGGCCCAGAT GATCTGCCCA 
    GCAGAAGAAG GCGAGAATAA CGACAAGTTC TTCACCCACC CCAAGAACGC CAAAGCTCTG
    GCGGCCTACC TCTTCGCACA CAACCACCTC TTCTACCTGA TGGAGCTGTC CGCGGCCTTG
    CTCCTGCTGC TGCTCTCCCT GTGCGAGGCT CCCGCCGTCC CCGCGCTCCG GCTGGGCATT
    TACGTCCACG CAACCCTGGA GCTGTTCGCC CTGGTGGTGG TCGTGTTTGA ACTCTGCATG
    AAGTTGCGGT GGCTGGGCCT TCACACCTTC ATCCGGCACA GAAGGACCAT GGTCAAGACC
    TCCGTGCTGG TGGTGCAGTT CGTGGAGGCC ATCGTGGTGT TGGTACGGCA GACGTCACAC
    ATGCGGGTGA CCCGGGCGCT GCGCTGCATC TTCCTGGTGG ACTGTCGGTA CTGCGGCGGC
    GTCCGGCGCA ACCTGCGTCA GATGTTTCAG TCCCTGCCGC CCTTCATGGA CATCCTCCTG
    CTTCTCCTCT TCTTCATGAT CATTTTCGCC ATCCTCGGTT TCTACTTATT CTCCCCTAAT
    CCTTCAGACC CCTACTTCAG CACCCTGGAG AACAGCATCA TCAGTCTGTT TGTCCTTCTG
    ACCACCGCCA ACTTCCCAGA CGTGATGATG CCCTCCTACT CCCGGAACCC CTGGTCCTGC
    GTCTTCTTCA TCGTGTACCT CTCCATTGAG CTGTACTTTA TCATGAACCT GCTCCTGGCC
    GTGGTATTTG ACACCTTCAA TGACATCGAG AAACGCAAGT TCAAGTCTTT GCTGCTACAC
    AAGCGAACTG CCATCCAGCA CGCCTACCGC CTGCTTGTCA GCCAGCGGAG GCCTGCCGGC
    ATCTCCTACA GGCAGTTCGA AGGCCTGATG CGTTTCTACA AGCCCCGGAT GAGTGCGGGG
    GAGCGCTATC TCACCTTCAA GGCCCTGAAT CAGAGCAACA CGCCACTGCT CAGCCTGAAG
    GACTTTCATG ATATTTATGA AGTTGCCGCC TTAAAGTGGA AGGCAAAGAG AAATAGAGAA
    CACTGGTTTG ATGAGCTTCC CAGGACAGCA TTCCTCATCT TCAAAGGAAT TAATATCCTT
    GTGAAGTCCA AGGCCTTCCA GTATTTCATG TATTTGGTGG TAGCGGTCAA CGGGGTCTGG
    ATCCTCGTGG AGACCTTCAT GCTGAAAGGT GGGAACTTCG TCTCCAGGCA CGTGCCGTGG
    AGTCACCTCG TCTTTCTGAC TATCTATGCG GTAGAGCTGT TCCTGAAAGT TGCCGGCCTG
    GGCCCCATTG AGTACCTGTC CTCAGGATGG AACCTGTTCG ACTTCTCCGT CACAGCCTTT
    GCCTTCCTGG GACTGCTGGC TCTGGCCTTC AACATGGAGC CCTTCTACTT CATAGTGGTC
    CTGCGTCCCC TCCAGCTGCT GAGGTTGTTT AAGCTGAAGA AGCGTTACCG CAACGTGCTG
    GACACCATGT TTGAGCTGCT GCCCCGGATG GCCAGCCTGG GCCTGACTCT GCTCATCTTC
    TACTACTCCT TCGCCATCGT GGGCATGGAA TTCTTCTATG GAATCCTCTA CCCCAACTGC
    TGCAATACAA GCACAGTGGC AGACGCCTAC CGCTGGCTGA ACCACTCCGT GGGCAACAGG
    ACCGTCGTGG AGGAGGGCTA TTACTATCTC AATAATTTTG ACAACATCCT GAACAGCTTT
    GTGACCTTGT TCGAGCTCAC GGTTGTCAAC AACTGGTACA TCATCATGGA AGGTGTCACC
    TCCCAGACCT CGCACTGGAG CCGCCTCTAC TTCATGACCT TCTACATCGT GACCATGGTG
    GTGATGACCA TCATTGTGGC CTTCATCCTC GAAGCCTTTG TCTTCCGAAT GAACTACAGC
    CGCAAGAACC AGGACTCGGA AGTGGACGAC GGCATCACCC TGGAGAAGGA GCTCTCCAAA
    GACGAGCTGG TCGCAGTTCT GAAGCTCTAC CGGGAGGCAG GGGGAGCCAA CTCAGACATT
    GCCCAGCTGC TCAAGATCCT CTCCCAGCTG GAGAGATACG AGCAAAATAC CTTGGTGTTT
    CTGGGACGGA GATCAAGGAC CAAAAGCGAC CTGAGCCTAA AGATGTACCA AGAGGAGATT
    CAGGAGTGGT ATGAGGAACA TGCCCGCAAG CAGGAGGAAC GACAGCTCAG CAGCTGCGTG
    GTCCCTGCCA CCCAGCAGCC TCCAGGCAGC CGCCAGCGCT CCCAGACCAT CACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322643.1
    CDS36..2312
    Translation

    Target ORF information:

    RefSeq Version XM_015467157.1
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467157.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGGGTGG CAGCGCCCCA CTGGCTCCCT CCAACGGCCT GGGCCCAGAT GATCTGCCCA 
    GCAGAAGAAG GCGAGAATAA CGACAAGTTC TTCACCCACC CCAAGAACGC CAAAGCTCTG
    GCGGCCTACC TCTTCGCACA CAACCACCTC TTCTACCTGA TGGAGCTGTC CGCGGCCTTG
    CTCCTGCTGC TGCTCTCCCT GTGCGAGGCT CCCGCCGTCC CCGCGCTCCG GCTGGGCATT
    TACGTCCACG CAACCCTGGA GCTGTTCGCC CTGGTGGTGG TCGTGTTTGA ACTCTGCATG
    AAGTTGCGGT GGCTGGGCCT TCACACCTTC ATCCGGCACA GAAGGACCAT GGTCAAGACC
    TCCGTGCTGG TGGTGCAGTT CGTGGAGGCC ATCGTGGTGT TGGTACGGCA GACGTCACAC
    ATGCGGGTGA CCCGGGCGCT GCGCTGCATC TTCCTGGTGG ACTGTCGGTA CTGCGGCGGC
    GTCCGGCGCA ACCTGCGTCA GATGTTTCAG TCCCTGCCGC CCTTCATGGA CATCCTCCTG
    CTTCTCCTCT TCTTCATGAT CATTTTCGCC ATCCTCGGTT TCTACTTATT CTCCCCTAAT
    CCTTCAGACC CCTACTTCAG CACCCTGGAG AACAGCATCA TCAGTCTGTT TGTCCTTCTG
    ACCACCGCCA ACTTCCCAGA CGTGATGATG CCCTCCTACT CCCGGAACCC CTGGTCCTGC
    GTCTTCTTCA TCGTGTACCT CTCCATTGAG CTGTACTTTA TCATGAACCT GCTCCTGGCC
    GTGGTATTTG ACACCTTCAA TGACATCGAG AAACGCAAGT TCAAGTCTTT GCTGCTACAC
    AAGCGAACTG CCATCCAGCA CGCCTACCGC CTGCTTGTCA GCCAGCGGAG GCCTGCCGGC
    ATCTCCTACA GGCAGTTCGA AGGCCTGATG CGTTTCTACA AGCCCCGGAT GAGTGCGGGG
    GAGCGCTATC TCACCTTCAA GGCCCTGAAT CAGAGCAACA CGCCACTGCT CAGCCTGAAG
    GACTTTCATG ATATTTATGA AGTTGCCGCC TTAAAGTGGA AGGCAAAGAG AAATAGAGAA
    CACTGGTTTG ATGAGCTTCC CAGGACAGCA TTCCTCATCT TCAAAGGAAT TAATATCCTT
    GTGAAGTCCA AGGCCTTCCA GTATTTCATG TATTTGGTGG TAGCGGTCAA CGGGGTCTGG
    ATCCTCGTGG AGACCTTCAT GCTGAAAGGT GGGAACTTCG TCTCCAGGCA CGTGCCGTGG
    AGTCACCTCG TCTTTCTGAC TATCTATGCG GTAGAGCTGT TCCTGAAAGT TGCCGGCCTG
    GGCCCCATTG AGTACCTGTC CTCAGGATGG AACCTGTTCG ACTTCTCCGT CACAGCCTTT
    GCCTTCCTGG GACTGCTGGC TCTGGCCTTC AACATGGAGC CCTTCTACTT CATAGTGGTC
    CTGCGTCCCC TCCAGCTGCT GAGGTTGTTT AAGCTGAAGA AGCGTTACCG CAACGTGCTG
    GACACCATGT TTGAGCTGCT GCCCCGGATG GCCAGCCTGG GCCTGACTCT GCTCATCTTC
    TACTACTCCT TCGCCATCGT GGGCATGGAA TTCTTCTATG GAATCCTCTA CCCCAACTGC
    TGCAATACAA GCACAGTGGC AGACGCCTAC CGCTGGCTGA ACCACTCCGT GGGCAACAGG
    ACCGTCGTGG AGGAGGGCTA TTACTATCTC AATAATTTTG ACAACATCCT GAACAGCTTT
    GTGACCTTGT TCGAGCTCAC GGTTGTCAAC AACTGGTACA TCATCATGGA AGGTGTCACC
    TCCCAGACCT CGCACTGGAG CCGCCTCTAC TTCATGACCT TCTACATCGT GACCATGGTG
    GTGATGACCA TCATTGTGGC CTTCATCCTC GAAGCCTTTG TCTTCCGAAT GAACTACAGC
    CGCAAGAACC AGGACTCGGA AGTGGACGAC GGCATCACCC TGGAGAAGGA GCTCTCCAAA
    GACGAGCTGG TCGCAGTTCT GAAGCTCTAC CGGGAGGCAG GGGGAGCCAA CTCAGACATT
    GCCCAGCTGC TCAAGATCCT CTCCCAGCTG GAGAGATACG AGCAAAATAC CTTGGTGTTT
    CTGGGACGGA GATCAAGGAC CAAAAGCGAC CTGAGCCTAA AGATGTACCA AGAGGAGATT
    CAGGAGTGGT ATGAGGAACA TGCCCGCAAG CAGGAGGAAC GACAGCTCAG CAGCTGCGTG
    GTCCCTGCCA CCCAGCAGCC TCCAGGCAGC CGCCAGCGCT CCCAGACCAT CACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93227
    Clone ID Related Accession (Same CDS sequence) XM_015475526.1 , XM_015467157.1 , XM_015475526.2
    Accession Version XM_015475526.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product two pore calcium channel protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475526.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGGGTGG CAGCGCCCCA CTGGCTCCCT CCAACGGCCT GGGCCCAGAT GATCTGCCCA 
    GCAGAAGAAG GCGAGAATAA CGACAAGTTC TTCACCCACC CCAAGAACGC CAAAGCTCTG
    GCGGCCTACC TCTTCGCACA CAACCACCTC TTCTACCTGA TGGAGCTGTC CGCGGCCTTG
    CTCCTGCTGC TGCTCTCCCT GTGCGAGGCT CCCGCCGTCC CCGCGCTCCG GCTGGGCATT
    TACGTCCACG CAACCCTGGA GCTGTTCGCC CTGGTGGTGG TCGTGTTTGA ACTCTGCATG
    AAGTTGCGGT GGCTGGGCCT TCACACCTTC ATCCGGCACA GAAGGACCAT GGTCAAGACC
    TCCGTGCTGG TGGTGCAGTT CGTGGAGGCC ATCGTGGTGT TGGTACGGCA GACGTCACAC
    ATGCGGGTGA CCCGGGCGCT GCGCTGCATC TTCCTGGTGG ACTGTCGGTA CTGCGGCGGC
    GTCCGGCGCA ACCTGCGTCA GATGTTTCAG TCCCTGCCGC CCTTCATGGA CATCCTCCTG
    CTTCTCCTCT TCTTCATGAT CATTTTCGCC ATCCTCGGTT TCTACTTATT CTCCCCTAAT
    CCTTCAGACC CCTACTTCAG CACCCTGGAG AACAGCATCA TCAGTCTGTT TGTCCTTCTG
    ACCACCGCCA ACTTCCCAGA CGTGATGATG CCCTCCTACT CCCGGAACCC CTGGTCCTGC
    GTCTTCTTCA TCGTGTACCT CTCCATTGAG CTGTACTTTA TCATGAACCT GCTCCTGGCC
    GTGGTATTTG ACACCTTCAA TGACATCGAG AAACGCAAGT TCAAGTCTTT GCTGCTACAC
    AAGCGAACTG CCATCCAGCA CGCCTACCGC CTGCTTGTCA GCCAGCGGAG GCCTGCCGGC
    ATCTCCTACA GGCAGTTCGA AGGCCTGATG CGTTTCTACA AGCCCCGGAT GAGTGCGGGG
    GAGCGCTATC TCACCTTCAA GGCCCTGAAT CAGAGCAACA CGCCACTGCT CAGCCTGAAG
    GACTTTCATG ATATTTATGA AGTTGCCGCC TTAAAGTGGA AGGCAAAGAG AAATAGAGAA
    CACTGGTTTG ATGAGCTTCC CAGGACAGCA TTCCTCATCT TCAAAGGAAT TAATATCCTT
    GTGAAGTCCA AGGCCTTCCA GTATTTCATG TATTTGGTGG TAGCGGTCAA CGGGGTCTGG
    ATCCTCGTGG AGACCTTCAT GCTGAAAGGT GGGAACTTCG TCTCCAGGCA CGTGCCGTGG
    AGTCACCTCG TCTTTCTGAC TATCTATGCG GTAGAGCTGT TCCTGAAAGT TGCCGGCCTG
    GGCCCCATTG AGTACCTGTC CTCAGGATGG AACCTGTTCG ACTTCTCCGT CACAGCCTTT
    GCCTTCCTGG GACTGCTGGC TCTGGCCTTC AACATGGAGC CCTTCTACTT CATAGTGGTC
    CTGCGTCCCC TCCAGCTGCT GAGGTTGTTT AAGCTGAAGA AGCGTTACCG CAACGTGCTG
    GACACCATGT TTGAGCTGCT GCCCCGGATG GCCAGCCTGG GCCTGACTCT GCTCATCTTC
    TACTACTCCT TCGCCATCGT GGGCATGGAA TTCTTCTATG GAATCCTCTA CCCCAACTGC
    TGCAATACAA GCACAGTGGC AGACGCCTAC CGCTGGCTGA ACCACTCCGT GGGCAACAGG
    ACCGTCGTGG AGGAGGGCTA TTACTATCTC AATAATTTTG ACAACATCCT GAACAGCTTT
    GTGACCTTGT TCGAGCTCAC GGTTGTCAAC AACTGGTACA TCATCATGGA AGGTGTCACC
    TCCCAGACCT CGCACTGGAG CCGCCTCTAC TTCATGACCT TCTACATCGT GACCATGGTG
    GTGATGACCA TCATTGTGGC CTTCATCCTC GAAGCCTTTG TCTTCCGAAT GAACTACAGC
    CGCAAGAACC AGGACTCGGA AGTGGACGAC GGCATCACCC TGGAGAAGGA GCTCTCCAAA
    GACGAGCTGG TCGCAGTTCT GAAGCTCTAC CGGGAGGCAG GGGGAGCCAA CTCAGACATT
    GCCCAGCTGC TCAAGATCCT CTCCCAGCTG GAGAGATACG AGCAAAATAC CTTGGTGTTT
    CTGGGACGGA GATCAAGGAC CAAAAGCGAC CTGAGCCTAA AGATGTACCA AGAGGAGATT
    CAGGAGTGGT ATGAGGAACA TGCCCGCAAG CAGGAGGAAC GACAGCTCAG CAGCTGCGTG
    GTCCCTGCCA CCCAGCAGCC TCCAGGCAGC CGCCAGCGCT CCCAGACCAT CACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331012.1
    CDS393..2669
    Translation

    Target ORF information:

    RefSeq Version XM_015475526.2
    Organism Bos taurus(cattle)
    Definition Bos taurus two pore segment channel 1 (TPCN1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475526.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGGGTGG CAGCGCCCCA CTGGCTCCCT CCAACGGCCT GGGCCCAGAT GATCTGCCCA 
    GCAGAAGAAG GCGAGAATAA CGACAAGTTC TTCACCCACC CCAAGAACGC CAAAGCTCTG
    GCGGCCTACC TCTTCGCACA CAACCACCTC TTCTACCTGA TGGAGCTGTC CGCGGCCTTG
    CTCCTGCTGC TGCTCTCCCT GTGCGAGGCT CCCGCCGTCC CCGCGCTCCG GCTGGGCATT
    TACGTCCACG CAACCCTGGA GCTGTTCGCC CTGGTGGTGG TCGTGTTTGA ACTCTGCATG
    AAGTTGCGGT GGCTGGGCCT TCACACCTTC ATCCGGCACA GAAGGACCAT GGTCAAGACC
    TCCGTGCTGG TGGTGCAGTT CGTGGAGGCC ATCGTGGTGT TGGTACGGCA GACGTCACAC
    ATGCGGGTGA CCCGGGCGCT GCGCTGCATC TTCCTGGTGG ACTGTCGGTA CTGCGGCGGC
    GTCCGGCGCA ACCTGCGTCA GATGTTTCAG TCCCTGCCGC CCTTCATGGA CATCCTCCTG
    CTTCTCCTCT TCTTCATGAT CATTTTCGCC ATCCTCGGTT TCTACTTATT CTCCCCTAAT
    CCTTCAGACC CCTACTTCAG CACCCTGGAG AACAGCATCA TCAGTCTGTT TGTCCTTCTG
    ACCACCGCCA ACTTCCCAGA CGTGATGATG CCCTCCTACT CCCGGAACCC CTGGTCCTGC
    GTCTTCTTCA TCGTGTACCT CTCCATTGAG CTGTACTTTA TCATGAACCT GCTCCTGGCC
    GTGGTATTTG ACACCTTCAA TGACATCGAG AAACGCAAGT TCAAGTCTTT GCTGCTACAC
    AAGCGAACTG CCATCCAGCA CGCCTACCGC CTGCTTGTCA GCCAGCGGAG GCCTGCCGGC
    ATCTCCTACA GGCAGTTCGA AGGCCTGATG CGTTTCTACA AGCCCCGGAT GAGTGCGGGG
    GAGCGCTATC TCACCTTCAA GGCCCTGAAT CAGAGCAACA CGCCACTGCT CAGCCTGAAG
    GACTTTCATG ATATTTATGA AGTTGCCGCC TTAAAGTGGA AGGCAAAGAG AAATAGAGAA
    CACTGGTTTG ATGAGCTTCC CAGGACAGCA TTCCTCATCT TCAAAGGAAT TAATATCCTT
    GTGAAGTCCA AGGCCTTCCA GTATTTCATG TATTTGGTGG TAGCGGTCAA CGGGGTCTGG
    ATCCTCGTGG AGACCTTCAT GCTGAAAGGT GGGAACTTCG TCTCCAGGCA CGTGCCGTGG
    AGTCACCTCG TCTTTCTGAC TATCTATGCG GTAGAGCTGT TCCTGAAAGT TGCCGGCCTG
    GGCCCCATTG AGTACCTGTC CTCAGGATGG AACCTGTTCG ACTTCTCCGT CACAGCCTTT
    GCCTTCCTGG GACTGCTGGC TCTGGCCTTC AACATGGAGC CCTTCTACTT CATAGTGGTC
    CTGCGTCCCC TCCAGCTGCT GAGGTTGTTT AAGCTGAAGA AGCGTTACCG CAACGTGCTG
    GACACCATGT TTGAGCTGCT GCCCCGGATG GCCAGCCTGG GCCTGACTCT GCTCATCTTC
    TACTACTCCT TCGCCATCGT GGGCATGGAA TTCTTCTATG GAATCCTCTA CCCCAACTGC
    TGCAATACAA GCACAGTGGC AGACGCCTAC CGCTGGCTGA ACCACTCCGT GGGCAACAGG
    ACCGTCGTGG AGGAGGGCTA TTACTATCTC AATAATTTTG ACAACATCCT GAACAGCTTT
    GTGACCTTGT TCGAGCTCAC GGTTGTCAAC AACTGGTACA TCATCATGGA AGGTGTCACC
    TCCCAGACCT CGCACTGGAG CCGCCTCTAC TTCATGACCT TCTACATCGT GACCATGGTG
    GTGATGACCA TCATTGTGGC CTTCATCCTC GAAGCCTTTG TCTTCCGAAT GAACTACAGC
    CGCAAGAACC AGGACTCGGA AGTGGACGAC GGCATCACCC TGGAGAAGGA GCTCTCCAAA
    GACGAGCTGG TCGCAGTTCT GAAGCTCTAC CGGGAGGCAG GGGGAGCCAA CTCAGACATT
    GCCCAGCTGC TCAAGATCCT CTCCCAGCTG GAGAGATACG AGCAAAATAC CTTGGTGTTT
    CTGGGACGGA GATCAAGGAC CAAAAGCGAC CTGAGCCTAA AGATGTACCA AGAGGAGATT
    CAGGAGTGGT ATGAGGAACA TGCCCGCAAG CAGGAGGAAC GACAGCTCAG CAGCTGCGTG
    GTCCCTGCCA CCCAGCAGCC TCCAGGCAGC CGCCAGCGCT CCCAGACCAT CACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.