TPCN1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	TPCN1
Entrez Gene ID	477492
Full Name	two pore segment channel 1
General protein information	Preferred Names two pore segment channel 1 Names two pore calcium channel protein 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005636255.2	XP_005636312.1	two pore calcium channel protein 1 isoform X2
XM_534690.6	XP_534690.2	two pore calcium channel protein 1 isoform X1
XM_005636255.3	XP_005636312.1	two pore calcium channel protein 1 isoform X2
XM_534690.5	XP_534690.2	two pore calcium channel protein 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TPCN1	XP_003313998.1
Canis lupus familiaris (dog)	TPCN1	XP_534690.2
Bos taurus (cattle)	TPCN1	XP_002694563.1
Gallus gallus (chicken)	TPCN1	XP_004934547.1
Homo sapiens (human)	TPCN1	NP_001137291.1
Rattus norvegicus (Norway rat)	Tpcn1	NP_647548.2
Danio rerio (zebrafish)	tpcn1	XP_005165277.1
Xenopus tropicalis (tropical clawed frog)	tpcn1	XP_002934333.2
	TPCN1	XP_002798827.1
Mus musculus (house mouse)	Tpcn1	NP_665852.1

Gene Ontology

The following TPCN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TPCN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe26278	XM_534690.6 Latest version!	Canis lupus familiaris two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe26279	XM_005636255.3 Latest version!	Canis lupus familiaris two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OCe26279	XM_005636255.2	Canis lupus familiaris two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe26278	XM_534690.5	Canis lupus familiaris two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TPCN1 ( XM_534690.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_534690.2 Canis lupus familiaris two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe26278
Clone ID Related Accession (Same CDS sequence)	XM_534690.6 , XM_534690.5
Accession Version	XM_534690.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	two pore calcium channel protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_534690.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGACGA GGGTGGCAGT 
GCCCCACTAG CTCCCTCCAA CAGCCTGGGC CAAGAGGAGC TGCCTAACAG AAATGGAGGC 
AGCTATGCTG TCCAGGACTC CCAGACCTCC AGCCTCGGCT TGGGGGGTGA GAGTCCCCCA 
TCAAGCCCCA CGGGACACAA CTGGGAGATC AATTATCAAG AGGCAGCAAT CTACCTCCAG 
GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC GCTGGCGGCC 
TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCACAGC TCTGCTCCTA 
TTGCTGCTGT CTCTGTGCGA GGCCCCTGCC GTCCCTGCGC TCCGGCTCGG CATTTACGTC 
CATGCGACCC TGGAGCTGTT TGCCCTGATG GTGGTTGTGT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCCTCCATAC CTTCATCCGG CATAAACGGA CCATGGTCAA GACCTCCGTG 
CTGGTGGTGC AGTTCATCGA GGCCATCGTG GTGTTGGTAC GGCAGACATC CCACGTGCGT 
GTGACCCGGG CGCTGCGCTG CATTTTCCTG GTGGACTGTC GGTACTGCGG GGGCGTCCGG 
CGCAACCTGC GGCAGATCTT CCAATCTCTG CCGCCCTTCA TGGACATCCT CCTGCTGCTG 
CTCTTTTTCA TGATCATTTT CGCCATCCTA GGCTTCTACT TGTTCTCCCC TAATCCTTCA 
GACCCTTACT TCAGCACGCT AGAGAACAGC ATTGTCAGTC TGTTTGTCCT TCTGACCACA 
GCCAATTTCC CAGATGTGAT GATGCCCTCC TACTCCCGGA ATCCCTGGTC CTGTGTCTTC 
TTCATCGTCT ACCTCTCCAT CGAGCTGTAC TTCATCATGA ACCTGCTTCT GGCTGTTGTG 
TTCGACACCT TCAATGATAT TGAGAAACGC AAGTTCAAGT CTTTGCTGCT GCATAAGCGA 
ACCGCCATCC AGCATGCCTA CTGCCTGCTC ATCAGCCAAC GGAGGCCCGC CGGCATCTCC 
TACAGGCAGT TTGAAGGCCT GATGCGCTTC TACAGGCCCC GGATGAGTGC CAGGGAGCGT 
TATCTTACTT TTAAGGCCCT GAATCAGAGC AACACACCTC TGCTCAGCCT GAAGGACTTT 
TATGATATTT ATGAAGTCGC TGCCTTGAAA TGGAAGGCAA AGAGAAATAG AGAACACTGG 
TTTGATGAGC TTCCCAGAAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAA 
TCCAAGGCCT TCCAGTACTT CATGTACTTG GTGGTGGCTG TCAATGGGGT CTGGATCCTC 
GTGGAGACCT TCATGCTGAA AGGCGGGAAC TTCTTCTCTA AGCACGTGCC CTGGAGTTAC 
GTCGTCTTTC TGACTATCTA CGGGGTGGAG CTATTCCTGA AGGTTGCTGG CCTTGGTCCC 
CTTGAATACC TGTCCTCCGG GTGGAATCTG TTCGACTTCT CTGTCACTCT GTTTGCCTTT 
CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTTT ATTTCATAGT GGTCCTGCGT 
CCCCTCCAGC TGCTCAGGTT GTTTAAGTTG AAAAAGCGCT ACCGTAATGT GCTGGACACC 
ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTCA CCCTGCTCAT CTTTTACTAC 
TCCTTGGCCA TCGTGGGCAT GGAATTCTTC TGTGGAATCC TCTACCCCAA CTGCTGCAAT 
ACGAGCACAG TATCTGATGC CTACCGCTGG CTCAATCACA CCGTGGGCAA CAGGACCGTT 
GTGGACGAAG GCTACTACTA TCTTAATAAC TTTGACAACA TCCTAAACAG CTTTGTGACC 
TTGTTCGAGC TCACAGTTGT CAACAACTGG TACATCATTA TGGAAGGTGT CACCTCTCAG 
ACCTCACACT GGAGCCGTCT GTACTTCATG ACTTTTTACA TCGTGACCAT GGTGGTGATG 
ACTATCATTG TCGCCTTCAT CCTTGAAGCC TTCGTCTTCC GAATGAACTA CAGCCGCAAG 
AACCAGGACT CGGAAGTTGA TGGTGGCATC ACCCTTGAGA AGGAAATCTC CAAAGATGAG 
CTGCTTGCTG TCCTGGAGCT CTACCGGGAG GTGCGAGGAG CCACCTCGGA TATCACCCGG 
CTGCTCACAG TCCTCTCTCA GATGGAGAAA CACGAGCAAA ACACCGTGGT GTTTCTGGGA 
CGGAGATCAA GGACCAAAAG TGACCTGAGC CTTAAGATGT ACCAAGAGGA GATTCAGGAG 
TGGTACGAGG AACACGCCCG CAAGCAAGAG GAGCAGCAGC GACAGCTCAG CAGCAGCGGC 
CAGGTCCCCA CCACCCAGCA GCTCCCAGGC AGCCGCCAGC GCTCCCAGAC CATCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_534690.2
CDS	282..2741
Translation	MAVSLDDDVPLILTLDEGGSAPLAPSNSLGQEELPNRNGGSYAVQDSQTSSLGLGGESPPSSPTGHNWEINYQEAAIYLQEGENNDKFFTHPKNAKALAAYLFAHNHLFYLMELSTALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYCLLISQRRPAGISYRQFEGLMRFYRPRMSARERYLTFKALNQSNTPLLSLKDFYDIYEVAALKWKAKRNREHWFDELPRTAFLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYVVFLTIYGVELFLKVAGLGPLEYLSSGWNLFDFSVTLFAFLGLLALAFNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLIFYYSLAIVGMEFFCGILYPNCCNTSTVSDAYRWLNHTVGNRTVVDEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKDELLAVLELYREVRGATSDITRLLTVLSQMEKHEQNTVVFLGRRSRTKSDLSLKMYQEEIQEWYEEHARKQEEQQRQLSSSGQVPTTQQLPGSRQRSQTIT

Target ORF information:

RefSeq Version	XM_534690.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_534690.6

ORF Insert Sequence:

ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGACGA GGGTGGCAGT 
GCCCCACTAG CTCCCTCCAA CAGCCTGGGC CAAGAGGAGC TGCCTAACAG AAATGGAGGC 
AGCTATGCTG TCCAGGACTC CCAGACCTCC AGCCTCGGCT TGGGGGGTGA GAGTCCCCCA 
TCAAGCCCCA CGGGACACAA CTGGGAGATC AATTATCAAG AGGCAGCAAT CTACCTCCAG 
GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC GCTGGCGGCC 
TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCACAGC TCTGCTCCTA 
TTGCTGCTGT CTCTGTGCGA GGCCCCTGCC GTCCCTGCGC TCCGGCTCGG CATTTACGTC 
CATGCGACCC TGGAGCTGTT TGCCCTGATG GTGGTTGTGT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCCTCCATAC CTTCATCCGG CATAAACGGA CCATGGTCAA GACCTCCGTG 
CTGGTGGTGC AGTTCATCGA GGCCATCGTG GTGTTGGTAC GGCAGACATC CCACGTGCGT 
GTGACCCGGG CGCTGCGCTG CATTTTCCTG GTGGACTGTC GGTACTGCGG GGGCGTCCGG 
CGCAACCTGC GGCAGATCTT CCAATCTCTG CCGCCCTTCA TGGACATCCT CCTGCTGCTG 
CTCTTTTTCA TGATCATTTT CGCCATCCTA GGCTTCTACT TGTTCTCCCC TAATCCTTCA 
GACCCTTACT TCAGCACGCT AGAGAACAGC ATTGTCAGTC TGTTTGTCCT TCTGACCACA 
GCCAATTTCC CAGATGTGAT GATGCCCTCC TACTCCCGGA ATCCCTGGTC CTGTGTCTTC 
TTCATCGTCT ACCTCTCCAT CGAGCTGTAC TTCATCATGA ACCTGCTTCT GGCTGTTGTG 
TTCGACACCT TCAATGATAT TGAGAAACGC AAGTTCAAGT CTTTGCTGCT GCATAAGCGA 
ACCGCCATCC AGCATGCCTA CTGCCTGCTC ATCAGCCAAC GGAGGCCCGC CGGCATCTCC 
TACAGGCAGT TTGAAGGCCT GATGCGCTTC TACAGGCCCC GGATGAGTGC CAGGGAGCGT 
TATCTTACTT TTAAGGCCCT GAATCAGAGC AACACACCTC TGCTCAGCCT GAAGGACTTT 
TATGATATTT ATGAAGTCGC TGCCTTGAAA TGGAAGGCAA AGAGAAATAG AGAACACTGG 
TTTGATGAGC TTCCCAGAAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAA 
TCCAAGGCCT TCCAGTACTT CATGTACTTG GTGGTGGCTG TCAATGGGGT CTGGATCCTC 
GTGGAGACCT TCATGCTGAA AGGCGGGAAC TTCTTCTCTA AGCACGTGCC CTGGAGTTAC 
GTCGTCTTTC TGACTATCTA CGGGGTGGAG CTATTCCTGA AGGTTGCTGG CCTTGGTCCC 
CTTGAATACC TGTCCTCCGG GTGGAATCTG TTCGACTTCT CTGTCACTCT GTTTGCCTTT 
CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTTT ATTTCATAGT GGTCCTGCGT 
CCCCTCCAGC TGCTCAGGTT GTTTAAGTTG AAAAAGCGCT ACCGTAATGT GCTGGACACC 
ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTCA CCCTGCTCAT CTTTTACTAC 
TCCTTGGCCA TCGTGGGCAT GGAATTCTTC TGTGGAATCC TCTACCCCAA CTGCTGCAAT 
ACGAGCACAG TATCTGATGC CTACCGCTGG CTCAATCACA CCGTGGGCAA CAGGACCGTT 
GTGGACGAAG GCTACTACTA TCTTAATAAC TTTGACAACA TCCTAAACAG CTTTGTGACC 
TTGTTCGAGC TCACAGTTGT CAACAACTGG TACATCATTA TGGAAGGTGT CACCTCTCAG 
ACCTCACACT GGAGCCGTCT GTACTTCATG ACTTTTTACA TCGTGACCAT GGTGGTGATG 
ACTATCATTG TCGCCTTCAT CCTTGAAGCC TTCGTCTTCC GAATGAACTA CAGCCGCAAG 
AACCAGGACT CGGAAGTTGA TGGTGGCATC ACCCTTGAGA AGGAAATCTC CAAAGATGAG 
CTGCTTGCTG TCCTGGAGCT CTACCGGGAG GTGCGAGGAG CCACCTCGGA TATCACCCGG 
CTGCTCACAG TCCTCTCTCA GATGGAGAAA CACGAGCAAA ACACCGTGGT GTTTCTGGGA 
CGGAGATCAA GGACCAAAAG TGACCTGAGC CTTAAGATGT ACCAAGAGGA GATTCAGGAG 
TGGTACGAGG AACACGCCCG CAAGCAAGAG GAGCAGCAGC GACAGCTCAG CAGCAGCGGC 
CAGGTCCCCA CCACCCAGCA GCTCCCAGGC AGCCGCCAGC GCTCCCAGAC CATCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_534690.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_534690.2 Canis lupus familiaris two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe26278
Clone ID Related Accession (Same CDS sequence)	XM_534690.6 , XM_534690.5
Accession Version	XM_534690.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	two pore calcium channel protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_534690.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGACGA GGGTGGCAGT 
GCCCCACTAG CTCCCTCCAA CAGCCTGGGC CAAGAGGAGC TGCCTAACAG AAATGGAGGC 
AGCTATGCTG TCCAGGACTC CCAGACCTCC AGCCTCGGCT TGGGGGGTGA GAGTCCCCCA 
TCAAGCCCCA CGGGACACAA CTGGGAGATC AATTATCAAG AGGCAGCAAT CTACCTCCAG 
GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC GCTGGCGGCC 
TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCACAGC TCTGCTCCTA 
TTGCTGCTGT CTCTGTGCGA GGCCCCTGCC GTCCCTGCGC TCCGGCTCGG CATTTACGTC 
CATGCGACCC TGGAGCTGTT TGCCCTGATG GTGGTTGTGT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCCTCCATAC CTTCATCCGG CATAAACGGA CCATGGTCAA GACCTCCGTG 
CTGGTGGTGC AGTTCATCGA GGCCATCGTG GTGTTGGTAC GGCAGACATC CCACGTGCGT 
GTGACCCGGG CGCTGCGCTG CATTTTCCTG GTGGACTGTC GGTACTGCGG GGGCGTCCGG 
CGCAACCTGC GGCAGATCTT CCAATCTCTG CCGCCCTTCA TGGACATCCT CCTGCTGCTG 
CTCTTTTTCA TGATCATTTT CGCCATCCTA GGCTTCTACT TGTTCTCCCC TAATCCTTCA 
GACCCTTACT TCAGCACGCT AGAGAACAGC ATTGTCAGTC TGTTTGTCCT TCTGACCACA 
GCCAATTTCC CAGATGTGAT GATGCCCTCC TACTCCCGGA ATCCCTGGTC CTGTGTCTTC 
TTCATCGTCT ACCTCTCCAT CGAGCTGTAC TTCATCATGA ACCTGCTTCT GGCTGTTGTG 
TTCGACACCT TCAATGATAT TGAGAAACGC AAGTTCAAGT CTTTGCTGCT GCATAAGCGA 
ACCGCCATCC AGCATGCCTA CTGCCTGCTC ATCAGCCAAC GGAGGCCCGC CGGCATCTCC 
TACAGGCAGT TTGAAGGCCT GATGCGCTTC TACAGGCCCC GGATGAGTGC CAGGGAGCGT 
TATCTTACTT TTAAGGCCCT GAATCAGAGC AACACACCTC TGCTCAGCCT GAAGGACTTT 
TATGATATTT ATGAAGTCGC TGCCTTGAAA TGGAAGGCAA AGAGAAATAG AGAACACTGG 
TTTGATGAGC TTCCCAGAAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAA 
TCCAAGGCCT TCCAGTACTT CATGTACTTG GTGGTGGCTG TCAATGGGGT CTGGATCCTC 
GTGGAGACCT TCATGCTGAA AGGCGGGAAC TTCTTCTCTA AGCACGTGCC CTGGAGTTAC 
GTCGTCTTTC TGACTATCTA CGGGGTGGAG CTATTCCTGA AGGTTGCTGG CCTTGGTCCC 
CTTGAATACC TGTCCTCCGG GTGGAATCTG TTCGACTTCT CTGTCACTCT GTTTGCCTTT 
CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTTT ATTTCATAGT GGTCCTGCGT 
CCCCTCCAGC TGCTCAGGTT GTTTAAGTTG AAAAAGCGCT ACCGTAATGT GCTGGACACC 
ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTCA CCCTGCTCAT CTTTTACTAC 
TCCTTGGCCA TCGTGGGCAT GGAATTCTTC TGTGGAATCC TCTACCCCAA CTGCTGCAAT 
ACGAGCACAG TATCTGATGC CTACCGCTGG CTCAATCACA CCGTGGGCAA CAGGACCGTT 
GTGGACGAAG GCTACTACTA TCTTAATAAC TTTGACAACA TCCTAAACAG CTTTGTGACC 
TTGTTCGAGC TCACAGTTGT CAACAACTGG TACATCATTA TGGAAGGTGT CACCTCTCAG 
ACCTCACACT GGAGCCGTCT GTACTTCATG ACTTTTTACA TCGTGACCAT GGTGGTGATG 
ACTATCATTG TCGCCTTCAT CCTTGAAGCC TTCGTCTTCC GAATGAACTA CAGCCGCAAG 
AACCAGGACT CGGAAGTTGA TGGTGGCATC ACCCTTGAGA AGGAAATCTC CAAAGATGAG 
CTGCTTGCTG TCCTGGAGCT CTACCGGGAG GTGCGAGGAG CCACCTCGGA TATCACCCGG 
CTGCTCACAG TCCTCTCTCA GATGGAGAAA CACGAGCAAA ACACCGTGGT GTTTCTGGGA 
CGGAGATCAA GGACCAAAAG TGACCTGAGC CTTAAGATGT ACCAAGAGGA GATTCAGGAG 
TGGTACGAGG AACACGCCCG CAAGCAAGAG GAGCAGCAGC GACAGCTCAG CAGCAGCGGC 
CAGGTCCCCA CCACCCAGCA GCTCCCAGGC AGCCGCCAGC GCTCCCAGAC CATCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_534690.2
CDS	301..2760
Translation	MAVSLDDDVPLILTLDEGGSAPLAPSNSLGQEELPNRNGGSYAVQDSQTSSLGLGGESPPSSPTGHNWEINYQEAAIYLQEGENNDKFFTHPKNAKALAAYLFAHNHLFYLMELSTALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYCLLISQRRPAGISYRQFEGLMRFYRPRMSARERYLTFKALNQSNTPLLSLKDFYDIYEVAALKWKAKRNREHWFDELPRTAFLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYVVFLTIYGVELFLKVAGLGPLEYLSSGWNLFDFSVTLFAFLGLLALAFNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLIFYYSLAIVGMEFFCGILYPNCCNTSTVSDAYRWLNHTVGNRTVVDEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKDELLAVLELYREVRGATSDITRLLTVLSQMEKHEQNTVVFLGRRSRTKSDLSLKMYQEEIQEWYEEHARKQEEQQRQLSSSGQVPTTQQLPGSRQRSQTIT

Target ORF information:

RefSeq Version	XM_534690.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_534690.5

ORF Insert Sequence:

ATGGCTGTGA GTTTAGATGA CGACGTTCCC CTCATCCTGA CCTTGGACGA GGGTGGCAGT 
GCCCCACTAG CTCCCTCCAA CAGCCTGGGC CAAGAGGAGC TGCCTAACAG AAATGGAGGC 
AGCTATGCTG TCCAGGACTC CCAGACCTCC AGCCTCGGCT TGGGGGGTGA GAGTCCCCCA 
TCAAGCCCCA CGGGACACAA CTGGGAGATC AATTATCAAG AGGCAGCAAT CTACCTCCAG 
GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC GCTGGCGGCC 
TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCACAGC TCTGCTCCTA 
TTGCTGCTGT CTCTGTGCGA GGCCCCTGCC GTCCCTGCGC TCCGGCTCGG CATTTACGTC 
CATGCGACCC TGGAGCTGTT TGCCCTGATG GTGGTTGTGT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCCTCCATAC CTTCATCCGG CATAAACGGA CCATGGTCAA GACCTCCGTG 
CTGGTGGTGC AGTTCATCGA GGCCATCGTG GTGTTGGTAC GGCAGACATC CCACGTGCGT 
GTGACCCGGG CGCTGCGCTG CATTTTCCTG GTGGACTGTC GGTACTGCGG GGGCGTCCGG 
CGCAACCTGC GGCAGATCTT CCAATCTCTG CCGCCCTTCA TGGACATCCT CCTGCTGCTG 
CTCTTTTTCA TGATCATTTT CGCCATCCTA GGCTTCTACT TGTTCTCCCC TAATCCTTCA 
GACCCTTACT TCAGCACGCT AGAGAACAGC ATTGTCAGTC TGTTTGTCCT TCTGACCACA 
GCCAATTTCC CAGATGTGAT GATGCCCTCC TACTCCCGGA ATCCCTGGTC CTGTGTCTTC 
TTCATCGTCT ACCTCTCCAT CGAGCTGTAC TTCATCATGA ACCTGCTTCT GGCTGTTGTG 
TTCGACACCT TCAATGATAT TGAGAAACGC AAGTTCAAGT CTTTGCTGCT GCATAAGCGA 
ACCGCCATCC AGCATGCCTA CTGCCTGCTC ATCAGCCAAC GGAGGCCCGC CGGCATCTCC 
TACAGGCAGT TTGAAGGCCT GATGCGCTTC TACAGGCCCC GGATGAGTGC CAGGGAGCGT 
TATCTTACTT TTAAGGCCCT GAATCAGAGC AACACACCTC TGCTCAGCCT GAAGGACTTT 
TATGATATTT ATGAAGTCGC TGCCTTGAAA TGGAAGGCAA AGAGAAATAG AGAACACTGG 
TTTGATGAGC TTCCCAGAAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAA 
TCCAAGGCCT TCCAGTACTT CATGTACTTG GTGGTGGCTG TCAATGGGGT CTGGATCCTC 
GTGGAGACCT TCATGCTGAA AGGCGGGAAC TTCTTCTCTA AGCACGTGCC CTGGAGTTAC 
GTCGTCTTTC TGACTATCTA CGGGGTGGAG CTATTCCTGA AGGTTGCTGG CCTTGGTCCC 
CTTGAATACC TGTCCTCCGG GTGGAATCTG TTCGACTTCT CTGTCACTCT GTTTGCCTTT 
CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTTT ATTTCATAGT GGTCCTGCGT 
CCCCTCCAGC TGCTCAGGTT GTTTAAGTTG AAAAAGCGCT ACCGTAATGT GCTGGACACC 
ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTCA CCCTGCTCAT CTTTTACTAC 
TCCTTGGCCA TCGTGGGCAT GGAATTCTTC TGTGGAATCC TCTACCCCAA CTGCTGCAAT 
ACGAGCACAG TATCTGATGC CTACCGCTGG CTCAATCACA CCGTGGGCAA CAGGACCGTT 
GTGGACGAAG GCTACTACTA TCTTAATAAC TTTGACAACA TCCTAAACAG CTTTGTGACC 
TTGTTCGAGC TCACAGTTGT CAACAACTGG TACATCATTA TGGAAGGTGT CACCTCTCAG 
ACCTCACACT GGAGCCGTCT GTACTTCATG ACTTTTTACA TCGTGACCAT GGTGGTGATG 
ACTATCATTG TCGCCTTCAT CCTTGAAGCC TTCGTCTTCC GAATGAACTA CAGCCGCAAG 
AACCAGGACT CGGAAGTTGA TGGTGGCATC ACCCTTGAGA AGGAAATCTC CAAAGATGAG 
CTGCTTGCTG TCCTGGAGCT CTACCGGGAG GTGCGAGGAG CCACCTCGGA TATCACCCGG 
CTGCTCACAG TCCTCTCTCA GATGGAGAAA CACGAGCAAA ACACCGTGGT GTTTCTGGGA 
CGGAGATCAA GGACCAAAAG TGACCTGAGC CTTAAGATGT ACCAAGAGGA GATTCAGGAG 
TGGTACGAGG AACACGCCCG CAAGCAAGAG GAGCAGCAGC GACAGCTCAG CAGCAGCGGC 
CAGGTCCCCA CCACCCAGCA GCTCCCAGGC AGCCGCCAGC GCTCCCAGAC CATCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_005636255.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005636312.1 Canis lupus familiaris two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe26279
Clone ID Related Accession (Same CDS sequence)	XM_005636255.2 , XM_005636255.3
Accession Version	XM_005636255.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	two pore calcium channel protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636255.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGGC CAGGGCCGCC AGCCATTGTC TTCCTGGAGG AGGTAGTGGA GGGCCAGCTC 
CCCAACAGCC CTGTGGTGAG GTCATCCAGT CTCCACCAGC TGGGCCCTTG CGATGGAGGC 
AGCTATGCTG TCCAGGACTC CCAGACCTCC AGCCTCGGCT TGGGGGGTGA GAGTCCCCCA 
TCAAGCCCCA CGGGACACAA CTGGGAGATC AATTATCAAG AGGCAGCAAT CTACCTCCAG 
GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC GCTGGCGGCC 
TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCACAGC TCTGCTCCTA 
TTGCTGCTGT CTCTGTGCGA GGCCCCTGCC GTCCCTGCGC TCCGGCTCGG CATTTACGTC 
CATGCGACCC TGGAGCTGTT TGCCCTGATG GTGGTTGTGT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCCTCCATAC CTTCATCCGG CATAAACGGA CCATGGTCAA GACCTCCGTG 
CTGGTGGTGC AGTTCATCGA GGCCATCGTG GTGTTGGTAC GGCAGACATC CCACGTGCGT 
GTGACCCGGG CGCTGCGCTG CATTTTCCTG GTGGACTGTC GGTACTGCGG GGGCGTCCGG 
CGCAACCTGC GGCAGATCTT CCAATCTCTG CCGCCCTTCA TGGACATCCT CCTGCTGCTG 
CTCTTTTTCA TGATCATTTT CGCCATCCTA GGCTTCTACT TGTTCTCCCC TAATCCTTCA 
GACCCTTACT TCAGCACGCT AGAGAACAGC ATTGTCAGTC TGTTTGTCCT TCTGACCACA 
GCCAATTTCC CAGATGTGAT GATGCCCTCC TACTCCCGGA ATCCCTGGTC CTGTGTCTTC 
TTCATCGTCT ACCTCTCCAT CGAGCTGTAC TTCATCATGA ACCTGCTTCT GGCTGTTGTG 
TTCGACACCT TCAATGATAT TGAGAAACGC AAGTTCAAGT CTTTGCTGCT GCATAAGCGA 
ACCGCCATCC AGCATGCCTA CTGCCTGCTC ATCAGCCAAC GGAGGCCCGC CGGCATCTCC 
TACAGGCAGT TTGAAGGCCT GATGCGCTTC TACAGGCCCC GGATGAGTGC CAGGGAGCGT 
TATCTTACTT TTAAGGCCCT GAATCAGAGC AACACACCTC TGCTCAGCCT GAAGGACTTT 
TATGATATTT ATGAAGTCGC TGCCTTGAAA TGGAAGGCAA AGAGAAATAG AGAACACTGG 
TTTGATGAGC TTCCCAGAAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAA 
TCCAAGGCCT TCCAGTACTT CATGTACTTG GTGGTGGCTG TCAATGGGGT CTGGATCCTC 
GTGGAGACCT TCATGCTGAA AGGCGGGAAC TTCTTCTCTA AGCACGTGCC CTGGAGTTAC 
GTCGTCTTTC TGACTATCTA CGGGGTGGAG CTATTCCTGA AGGTTGCTGG CCTTGGTCCC 
CTTGAATACC TGTCCTCCGG GTGGAATCTG TTCGACTTCT CTGTCACTCT GTTTGCCTTT 
CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTTT ATTTCATAGT GGTCCTGCGT 
CCCCTCCAGC TGCTCAGGTT GTTTAAGTTG AAAAAGCGCT ACCGTAATGT GCTGGACACC 
ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTCA CCCTGCTCAT CTTTTACTAC 
TCCTTGGCCA TCGTGGGCAT GGAATTCTTC TGTGGAATCC TCTACCCCAA CTGCTGCAAT 
ACGAGCACAG TATCTGATGC CTACCGCTGG CTCAATCACA CCGTGGGCAA CAGGACCGTT 
GTGGACGAAG GCTACTACTA TCTTAATAAC TTTGACAACA TCCTAAACAG CTTTGTGACC 
TTGTTCGAGC TCACAGTTGT CAACAACTGG TACATCATTA TGGAAGGTGT CACCTCTCAG 
ACCTCACACT GGAGCCGTCT GTACTTCATG ACTTTTTACA TCGTGACCAT GGTGGTGATG 
ACTATCATTG TCGCCTTCAT CCTTGAAGCC TTCGTCTTCC GAATGAACTA CAGCCGCAAG 
AACCAGGACT CGGAAGTTGA TGGTGGCATC ACCCTTGAGA AGGAAATCTC CAAAGATGAG 
CTGCTTGCTG TCCTGGAGCT CTACCGGGAG GTGCGAGGAG CCACCTCGGA TATCACCCGG 
CTGCTCACAG TCCTCTCTCA GATGGAGAAA CACGAGCAAA ACACCGTGGT GTTTCTGGGA 
CGGAGATCAA GGACCAAAAG TGACCTGAGC CTTAAGATGT ACCAAGAGGA GATTCAGGAG 
TGGTACGAGG AACACGCCCG CAAGCAAGAG GAGCAGCAGC GACAGCTCAG CAGCAGCGGC 
CAGGTCCCCA CCACCCAGCA GCTCCCAGGC AGCCGCCAGC GCTCCCAGAC CATCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005636312.1
CDS	36..2495
Translation	MQRPGPPAIVFLEEVVEGQLPNSPVVRSSSLHQLGPCDGGSYAVQDSQTSSLGLGGESPPSSPTGHNWEINYQEAAIYLQEGENNDKFFTHPKNAKALAAYLFAHNHLFYLMELSTALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYCLLISQRRPAGISYRQFEGLMRFYRPRMSARERYLTFKALNQSNTPLLSLKDFYDIYEVAALKWKAKRNREHWFDELPRTAFLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYVVFLTIYGVELFLKVAGLGPLEYLSSGWNLFDFSVTLFAFLGLLALAFNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLIFYYSLAIVGMEFFCGILYPNCCNTSTVSDAYRWLNHTVGNRTVVDEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKDELLAVLELYREVRGATSDITRLLTVLSQMEKHEQNTVVFLGRRSRTKSDLSLKMYQEEIQEWYEEHARKQEEQQRQLSSSGQVPTTQQLPGSRQRSQTIT

Target ORF information:

RefSeq Version	XM_005636255.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005636255.2

ORF Insert Sequence:

ATGCAGAGGC CAGGGCCGCC AGCCATTGTC TTCCTGGAGG AGGTAGTGGA GGGCCAGCTC 
CCCAACAGCC CTGTGGTGAG GTCATCCAGT CTCCACCAGC TGGGCCCTTG CGATGGAGGC 
AGCTATGCTG TCCAGGACTC CCAGACCTCC AGCCTCGGCT TGGGGGGTGA GAGTCCCCCA 
TCAAGCCCCA CGGGACACAA CTGGGAGATC AATTATCAAG AGGCAGCAAT CTACCTCCAG 
GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC GCTGGCGGCC 
TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCACAGC TCTGCTCCTA 
TTGCTGCTGT CTCTGTGCGA GGCCCCTGCC GTCCCTGCGC TCCGGCTCGG CATTTACGTC 
CATGCGACCC TGGAGCTGTT TGCCCTGATG GTGGTTGTGT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCCTCCATAC CTTCATCCGG CATAAACGGA CCATGGTCAA GACCTCCGTG 
CTGGTGGTGC AGTTCATCGA GGCCATCGTG GTGTTGGTAC GGCAGACATC CCACGTGCGT 
GTGACCCGGG CGCTGCGCTG CATTTTCCTG GTGGACTGTC GGTACTGCGG GGGCGTCCGG 
CGCAACCTGC GGCAGATCTT CCAATCTCTG CCGCCCTTCA TGGACATCCT CCTGCTGCTG 
CTCTTTTTCA TGATCATTTT CGCCATCCTA GGCTTCTACT TGTTCTCCCC TAATCCTTCA 
GACCCTTACT TCAGCACGCT AGAGAACAGC ATTGTCAGTC TGTTTGTCCT TCTGACCACA 
GCCAATTTCC CAGATGTGAT GATGCCCTCC TACTCCCGGA ATCCCTGGTC CTGTGTCTTC 
TTCATCGTCT ACCTCTCCAT CGAGCTGTAC TTCATCATGA ACCTGCTTCT GGCTGTTGTG 
TTCGACACCT TCAATGATAT TGAGAAACGC AAGTTCAAGT CTTTGCTGCT GCATAAGCGA 
ACCGCCATCC AGCATGCCTA CTGCCTGCTC ATCAGCCAAC GGAGGCCCGC CGGCATCTCC 
TACAGGCAGT TTGAAGGCCT GATGCGCTTC TACAGGCCCC GGATGAGTGC CAGGGAGCGT 
TATCTTACTT TTAAGGCCCT GAATCAGAGC AACACACCTC TGCTCAGCCT GAAGGACTTT 
TATGATATTT ATGAAGTCGC TGCCTTGAAA TGGAAGGCAA AGAGAAATAG AGAACACTGG 
TTTGATGAGC TTCCCAGAAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAA 
TCCAAGGCCT TCCAGTACTT CATGTACTTG GTGGTGGCTG TCAATGGGGT CTGGATCCTC 
GTGGAGACCT TCATGCTGAA AGGCGGGAAC TTCTTCTCTA AGCACGTGCC CTGGAGTTAC 
GTCGTCTTTC TGACTATCTA CGGGGTGGAG CTATTCCTGA AGGTTGCTGG CCTTGGTCCC 
CTTGAATACC TGTCCTCCGG GTGGAATCTG TTCGACTTCT CTGTCACTCT GTTTGCCTTT 
CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTTT ATTTCATAGT GGTCCTGCGT 
CCCCTCCAGC TGCTCAGGTT GTTTAAGTTG AAAAAGCGCT ACCGTAATGT GCTGGACACC 
ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTCA CCCTGCTCAT CTTTTACTAC 
TCCTTGGCCA TCGTGGGCAT GGAATTCTTC TGTGGAATCC TCTACCCCAA CTGCTGCAAT 
ACGAGCACAG TATCTGATGC CTACCGCTGG CTCAATCACA CCGTGGGCAA CAGGACCGTT 
GTGGACGAAG GCTACTACTA TCTTAATAAC TTTGACAACA TCCTAAACAG CTTTGTGACC 
TTGTTCGAGC TCACAGTTGT CAACAACTGG TACATCATTA TGGAAGGTGT CACCTCTCAG 
ACCTCACACT GGAGCCGTCT GTACTTCATG ACTTTTTACA TCGTGACCAT GGTGGTGATG 
ACTATCATTG TCGCCTTCAT CCTTGAAGCC TTCGTCTTCC GAATGAACTA CAGCCGCAAG 
AACCAGGACT CGGAAGTTGA TGGTGGCATC ACCCTTGAGA AGGAAATCTC CAAAGATGAG 
CTGCTTGCTG TCCTGGAGCT CTACCGGGAG GTGCGAGGAG CCACCTCGGA TATCACCCGG 
CTGCTCACAG TCCTCTCTCA GATGGAGAAA CACGAGCAAA ACACCGTGGT GTTTCTGGGA 
CGGAGATCAA GGACCAAAAG TGACCTGAGC CTTAAGATGT ACCAAGAGGA GATTCAGGAG 
TGGTACGAGG AACACGCCCG CAAGCAAGAG GAGCAGCAGC GACAGCTCAG CAGCAGCGGC 
CAGGTCCCCA CCACCCAGCA GCTCCCAGGC AGCCGCCAGC GCTCCCAGAC CATCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_005636255.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005636312.1 Canis lupus familiaris two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe26279
Clone ID Related Accession (Same CDS sequence)	XM_005636255.2 , XM_005636255.3
Accession Version	XM_005636255.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	two pore calcium channel protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636255.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGGC CAGGGCCGCC AGCCATTGTC TTCCTGGAGG AGGTAGTGGA GGGCCAGCTC 
CCCAACAGCC CTGTGGTGAG GTCATCCAGT CTCCACCAGC TGGGCCCTTG CGATGGAGGC 
AGCTATGCTG TCCAGGACTC CCAGACCTCC AGCCTCGGCT TGGGGGGTGA GAGTCCCCCA 
TCAAGCCCCA CGGGACACAA CTGGGAGATC AATTATCAAG AGGCAGCAAT CTACCTCCAG 
GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC GCTGGCGGCC 
TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCACAGC TCTGCTCCTA 
TTGCTGCTGT CTCTGTGCGA GGCCCCTGCC GTCCCTGCGC TCCGGCTCGG CATTTACGTC 
CATGCGACCC TGGAGCTGTT TGCCCTGATG GTGGTTGTGT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCCTCCATAC CTTCATCCGG CATAAACGGA CCATGGTCAA GACCTCCGTG 
CTGGTGGTGC AGTTCATCGA GGCCATCGTG GTGTTGGTAC GGCAGACATC CCACGTGCGT 
GTGACCCGGG CGCTGCGCTG CATTTTCCTG GTGGACTGTC GGTACTGCGG GGGCGTCCGG 
CGCAACCTGC GGCAGATCTT CCAATCTCTG CCGCCCTTCA TGGACATCCT CCTGCTGCTG 
CTCTTTTTCA TGATCATTTT CGCCATCCTA GGCTTCTACT TGTTCTCCCC TAATCCTTCA 
GACCCTTACT TCAGCACGCT AGAGAACAGC ATTGTCAGTC TGTTTGTCCT TCTGACCACA 
GCCAATTTCC CAGATGTGAT GATGCCCTCC TACTCCCGGA ATCCCTGGTC CTGTGTCTTC 
TTCATCGTCT ACCTCTCCAT CGAGCTGTAC TTCATCATGA ACCTGCTTCT GGCTGTTGTG 
TTCGACACCT TCAATGATAT TGAGAAACGC AAGTTCAAGT CTTTGCTGCT GCATAAGCGA 
ACCGCCATCC AGCATGCCTA CTGCCTGCTC ATCAGCCAAC GGAGGCCCGC CGGCATCTCC 
TACAGGCAGT TTGAAGGCCT GATGCGCTTC TACAGGCCCC GGATGAGTGC CAGGGAGCGT 
TATCTTACTT TTAAGGCCCT GAATCAGAGC AACACACCTC TGCTCAGCCT GAAGGACTTT 
TATGATATTT ATGAAGTCGC TGCCTTGAAA TGGAAGGCAA AGAGAAATAG AGAACACTGG 
TTTGATGAGC TTCCCAGAAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAA 
TCCAAGGCCT TCCAGTACTT CATGTACTTG GTGGTGGCTG TCAATGGGGT CTGGATCCTC 
GTGGAGACCT TCATGCTGAA AGGCGGGAAC TTCTTCTCTA AGCACGTGCC CTGGAGTTAC 
GTCGTCTTTC TGACTATCTA CGGGGTGGAG CTATTCCTGA AGGTTGCTGG CCTTGGTCCC 
CTTGAATACC TGTCCTCCGG GTGGAATCTG TTCGACTTCT CTGTCACTCT GTTTGCCTTT 
CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTTT ATTTCATAGT GGTCCTGCGT 
CCCCTCCAGC TGCTCAGGTT GTTTAAGTTG AAAAAGCGCT ACCGTAATGT GCTGGACACC 
ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTCA CCCTGCTCAT CTTTTACTAC 
TCCTTGGCCA TCGTGGGCAT GGAATTCTTC TGTGGAATCC TCTACCCCAA CTGCTGCAAT 
ACGAGCACAG TATCTGATGC CTACCGCTGG CTCAATCACA CCGTGGGCAA CAGGACCGTT 
GTGGACGAAG GCTACTACTA TCTTAATAAC TTTGACAACA TCCTAAACAG CTTTGTGACC 
TTGTTCGAGC TCACAGTTGT CAACAACTGG TACATCATTA TGGAAGGTGT CACCTCTCAG 
ACCTCACACT GGAGCCGTCT GTACTTCATG ACTTTTTACA TCGTGACCAT GGTGGTGATG 
ACTATCATTG TCGCCTTCAT CCTTGAAGCC TTCGTCTTCC GAATGAACTA CAGCCGCAAG 
AACCAGGACT CGGAAGTTGA TGGTGGCATC ACCCTTGAGA AGGAAATCTC CAAAGATGAG 
CTGCTTGCTG TCCTGGAGCT CTACCGGGAG GTGCGAGGAG CCACCTCGGA TATCACCCGG 
CTGCTCACAG TCCTCTCTCA GATGGAGAAA CACGAGCAAA ACACCGTGGT GTTTCTGGGA 
CGGAGATCAA GGACCAAAAG TGACCTGAGC CTTAAGATGT ACCAAGAGGA GATTCAGGAG 
TGGTACGAGG AACACGCCCG CAAGCAAGAG GAGCAGCAGC GACAGCTCAG CAGCAGCGGC 
CAGGTCCCCA CCACCCAGCA GCTCCCAGGC AGCCGCCAGC GCTCCCAGAC CATCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005636312.1
CDS	27..2486
Translation	MQRPGPPAIVFLEEVVEGQLPNSPVVRSSSLHQLGPCDGGSYAVQDSQTSSLGLGGESPPSSPTGHNWEINYQEAAIYLQEGENNDKFFTHPKNAKALAAYLFAHNHLFYLMELSTALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYCLLISQRRPAGISYRQFEGLMRFYRPRMSARERYLTFKALNQSNTPLLSLKDFYDIYEVAALKWKAKRNREHWFDELPRTAFLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYVVFLTIYGVELFLKVAGLGPLEYLSSGWNLFDFSVTLFAFLGLLALAFNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLIFYYSLAIVGMEFFCGILYPNCCNTSTVSDAYRWLNHTVGNRTVVDEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKDELLAVLELYREVRGATSDITRLLTVLSQMEKHEQNTVVFLGRRSRTKSDLSLKMYQEEIQEWYEEHARKQEEQQRQLSSSGQVPTTQQLPGSRQRSQTIT

Target ORF information:

RefSeq Version	XM_005636255.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005636255.3

ORF Insert Sequence:

ATGCAGAGGC CAGGGCCGCC AGCCATTGTC TTCCTGGAGG AGGTAGTGGA GGGCCAGCTC 
CCCAACAGCC CTGTGGTGAG GTCATCCAGT CTCCACCAGC TGGGCCCTTG CGATGGAGGC 
AGCTATGCTG TCCAGGACTC CCAGACCTCC AGCCTCGGCT TGGGGGGTGA GAGTCCCCCA 
TCAAGCCCCA CGGGACACAA CTGGGAGATC AATTATCAAG AGGCAGCAAT CTACCTCCAG 
GAAGGCGAGA ATAACGACAA GTTCTTCACC CACCCCAAGA ACGCCAAAGC GCTGGCGGCC 
TACCTCTTCG CACACAACCA CCTCTTCTAC CTGATGGAGC TGTCCACAGC TCTGCTCCTA 
TTGCTGCTGT CTCTGTGCGA GGCCCCTGCC GTCCCTGCGC TCCGGCTCGG CATTTACGTC 
CATGCGACCC TGGAGCTGTT TGCCCTGATG GTGGTTGTGT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCCTCCATAC CTTCATCCGG CATAAACGGA CCATGGTCAA GACCTCCGTG 
CTGGTGGTGC AGTTCATCGA GGCCATCGTG GTGTTGGTAC GGCAGACATC CCACGTGCGT 
GTGACCCGGG CGCTGCGCTG CATTTTCCTG GTGGACTGTC GGTACTGCGG GGGCGTCCGG 
CGCAACCTGC GGCAGATCTT CCAATCTCTG CCGCCCTTCA TGGACATCCT CCTGCTGCTG 
CTCTTTTTCA TGATCATTTT CGCCATCCTA GGCTTCTACT TGTTCTCCCC TAATCCTTCA 
GACCCTTACT TCAGCACGCT AGAGAACAGC ATTGTCAGTC TGTTTGTCCT TCTGACCACA 
GCCAATTTCC CAGATGTGAT GATGCCCTCC TACTCCCGGA ATCCCTGGTC CTGTGTCTTC 
TTCATCGTCT ACCTCTCCAT CGAGCTGTAC TTCATCATGA ACCTGCTTCT GGCTGTTGTG 
TTCGACACCT TCAATGATAT TGAGAAACGC AAGTTCAAGT CTTTGCTGCT GCATAAGCGA 
ACCGCCATCC AGCATGCCTA CTGCCTGCTC ATCAGCCAAC GGAGGCCCGC CGGCATCTCC 
TACAGGCAGT TTGAAGGCCT GATGCGCTTC TACAGGCCCC GGATGAGTGC CAGGGAGCGT 
TATCTTACTT TTAAGGCCCT GAATCAGAGC AACACACCTC TGCTCAGCCT GAAGGACTTT 
TATGATATTT ATGAAGTCGC TGCCTTGAAA TGGAAGGCAA AGAGAAATAG AGAACACTGG 
TTTGATGAGC TTCCCAGAAC AGCATTCCTC ATCTTCAAAG GAATTAATAT CCTTGTGAAA 
TCCAAGGCCT TCCAGTACTT CATGTACTTG GTGGTGGCTG TCAATGGGGT CTGGATCCTC 
GTGGAGACCT TCATGCTGAA AGGCGGGAAC TTCTTCTCTA AGCACGTGCC CTGGAGTTAC 
GTCGTCTTTC TGACTATCTA CGGGGTGGAG CTATTCCTGA AGGTTGCTGG CCTTGGTCCC 
CTTGAATACC TGTCCTCCGG GTGGAATCTG TTCGACTTCT CTGTCACTCT GTTTGCCTTT 
CTGGGACTGC TGGCTCTGGC CTTCAACATG GAGCCCTTTT ATTTCATAGT GGTCCTGCGT 
CCCCTCCAGC TGCTCAGGTT GTTTAAGTTG AAAAAGCGCT ACCGTAATGT GCTGGACACC 
ATGTTTGAGC TGCTGCCCCG GATGGCCAGC CTGGGCCTCA CCCTGCTCAT CTTTTACTAC 
TCCTTGGCCA TCGTGGGCAT GGAATTCTTC TGTGGAATCC TCTACCCCAA CTGCTGCAAT 
ACGAGCACAG TATCTGATGC CTACCGCTGG CTCAATCACA CCGTGGGCAA CAGGACCGTT 
GTGGACGAAG GCTACTACTA TCTTAATAAC TTTGACAACA TCCTAAACAG CTTTGTGACC 
TTGTTCGAGC TCACAGTTGT CAACAACTGG TACATCATTA TGGAAGGTGT CACCTCTCAG 
ACCTCACACT GGAGCCGTCT GTACTTCATG ACTTTTTACA TCGTGACCAT GGTGGTGATG 
ACTATCATTG TCGCCTTCAT CCTTGAAGCC TTCGTCTTCC GAATGAACTA CAGCCGCAAG 
AACCAGGACT CGGAAGTTGA TGGTGGCATC ACCCTTGAGA AGGAAATCTC CAAAGATGAG 
CTGCTTGCTG TCCTGGAGCT CTACCGGGAG GTGCGAGGAG CCACCTCGGA TATCACCCGG 
CTGCTCACAG TCCTCTCTCA GATGGAGAAA CACGAGCAAA ACACCGTGGT GTTTCTGGGA 
CGGAGATCAA GGACCAAAAG TGACCTGAGC CTTAAGATGT ACCAAGAGGA GATTCAGGAG 
TGGTACGAGG AACACGCCCG CAAGCAAGAG GAGCAGCAGC GACAGCTCAG CAGCAGCGGC 
CAGGTCCCCA CCACCCAGCA GCTCCCAGGC AGCCGCCAGC GCTCCCAGAC CATCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TPCN1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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